LOC100161509 cDNA ORF clone, Acyrthosiphon pisum(pea aphid)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100161509
Entrez Gene ID	100161509
Full Name	transcription initiation factor TFIID subunit 2
Gene Type	protein-coding
Organism	Acyrthosiphon pisum(pea aphid)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003243754.4	XP_003243802.1	transcription initiation factor TFIID subunit 2
XM_003243754.3	XP_003243802.1	transcription initiation factor TFIID subunit 2

Pathways from BioSystems
Gene Ontology

The following LOC100161509 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100161509 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAd07905	XM_003243754.4 Latest version!	Acyrthosiphon pisum transcription initiation factor TFIID subunit 2 (LOC100161509), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OAd07905	XM_003243754.3	Acyrthosiphon pisum transcription initiation factor TFIID subunit 2 (LOC100161509), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100161509 ( XM_003243754.4 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_003243802.1 Acyrthosiphon pisum transcription initiation factor TFIID subunit 2 (LOC100161509), mRNA.

Price & Availability↑

CloneID	OAd07905
Clone ID Related Accession (Same CDS sequence)	XM_003243754.4 , XM_003243754.3
Accession Version	XM_003243754.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1560441600000
Organism	Acyrthosiphon pisum(pea aphid)
Product	transcription initiation factor TFIID subunit 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042496.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2019 this sequence version replaced XM_003243754.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acyrthosiphon pisum Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGG ATAAAACAGG AGACACCGCA AGGCCTTATA AACTAGCCCA TCAATTGGTT 
AGTTTGACAG GCATTAGTTT TCAACGAAAA AATATATTGG GAATGGTAGA GCTAACAATT 
ATACCATTGA AAAATAATCT TCAATTTATA AAACTAAATG CAAAACAATG TCGTATATAT 
CGCGTAACTG TTAATGAAAA CATTGAAGTT CCTTTTCAAT ATTTTGATCC ATTTGTTGAC 
ATTACTCAAA ATAAAGATGA AGAACCTACA TTAACTGCAT TTTCTGCAGC TCATTTAAAT 
GCTGCTCAGA TAACTGAACC CGATAACAAT AATGGGGAAT TAGTTATAAG TCTACCTGCA 
GAAATTACAA CATGTGTGAG TGATGGAACA AGTATTAAAA TAAGTGTAGA ATATTCTTTG 
GAACAGCCTA GTGGAGGTGT CCATTTTGTT ATTCCAGAAG GAGATGGATC TTTAGTTGAG 
AGACGAGCTC ATATGTTTAC TATGTGTTTT GAAAACTCAT CTCGACTATG GTTTCCGTGT 
GTCGATAGTT ATGCGGAACC ATGTACATGG GATTTAGAAT TTACAGTTGA TGAAAATATG 
ACGGCAGTAT CTTGTGGAGA TTTAATTGAA GTTGTTGCAG CTCCTGATTT AAGGCGTAAA 
ACATATTATT ATAGTTTATC AATGCCCACT TGTGCACCAA ATATAGCCTT AGCAGTGGGA 
CCTTTTGAAA TTTATGTGGA CCCTACGATG CATATTATAA CTCACTTCTG TTTGCCGGGG 
CTTTCTGAAC TTCTACCTAC AGCTGCAAAG TACACCCATG AAGCATTAGA ATTTTATGAA 
GATACGTTAT CTAACGGTTA TCCTTATAGT TGTTATAAAC AAGTATTTGT TGATGAAACT 
CCTACAGATT ATGCATCGTA TGCATCAATG GGTATTATGA GTGTTAATCT TCTACATCCA 
AAACAAATTA TTGATCAAGT ATATATTTCT AAAAAAATCT TGGCACAAGT TATGGCAGAG 
CAATTTTTCG GTTGTTTTAT TTCTACTCAA AATTGGTCTG ATTGGTGGCT AACGTGCGGT 
ATAGCATTAT ATATGTCTGG TCTTTTTGCA AAAAAATGTT TTGGCAATAA TTCTTATCGT 
GAATGGATTC ACAATGAATT AACAAGTTTG GTACGATATG AAGAAGAAAT AGGACCTATA 
GTGTTAGATC CAACCCAACA ACCTGCTCCA ACAAGTAGTG GTCTTGTCAG TCCAGGAGGA 
ACTTCTAATC CTGGATTTTC ATTATCTTTT AATAATGTGC AAAATACTAA TGAAACCTCA 
AATTTTTATT TCTCTACACG TAGCTTACAC ACAATATCAC CTCGTTATTT TCATTACATG 
ACTAAAAAGG CACATCTTGT ATTAAGAATG TTAGAAAATC GAATTGGCTA TTCATTATTG 
TTGCAGGTTT TGAACAAGCA TTTATCTTTA GCAAGCAATG CATCTCAAAA TCCAATGAAC 
TCAGGTTTAT GGGGCCACAT GTTAATTAGT ACAAATATAT TTAACAAGAC TATTTTTACT 
GTATCAGGCA TAGATATGTC TGTATTTATT GATCAATGGG TTAGAACTGG TGGTCACGCT 
AAATTTACTT GTACCTTTGT GTTCAATAGA AAAAGGAATA CATTGGAATT AGAAATTAAA 
CAAGATGCTG TTTGTCAACG AGGTATTCGT AAATATGTTG GCCCATTATT AGTTAGCATT 
CAAGAATTGG ATGGTCCATT TAAACATACT TTGCAAGTTG AAGGTAATGT AGCTCGAGCA 
GATATTACCT GTCATTCAAA AAGTAGGCGT AATAAGAAAA AGAAAATTCC TTTGTGTACG 
GGGGATGAAG TAGATATTGA TTTAAATGCT ATGGATAATG ACTCACCAGT ATTATGGATT 
CGATTAGATC CTGAGATGAC TATTTTACGC AGTTGTATTA TTGAACAACC TGAGTACCAG 
TGGAATTACC AATTGCGACA TGAAAAAGAT GTAACTGCCC AAATGGAAGC AGTGGTAGCT 
CTTGATAAAT TTCCTACAAT TGCTACACGT AATGCCTTAT GTGATATTAT TGAAAACGAA 
CAAGTGTATA TTCATGTACG GTGTAAAGCT GCTCACTGTT TAACTAAGAT TGCAAATGCA 
ATGGTTACTA ATTGGACTGG GCCTCCAGCA ATGTTGGCAA CATTTAAAAA AATGTTTGGT 
TCATTTTCAT GTCCACATAT AATAAAACAA AATAATTTCT CAAATCTACA ACATTACTAT 
CTACAGAAGA CAATTCCAAT TGCAATGGCT GGTTTGAGAA ATTCTCATGG AATTTGTCCC 
CCTGAAATCT TGAAATTCAT ATTAGATCTA TTCAAGTACA ATGATAATAG TAAAAATAGA 
TATTCTGATA ATTATTATAG AGCTTCACTG ATTGATGCTC TTGGTGCAAC AATTACACCA 
GTTATATCTT TAATGCAAAA AGCCTGTGAT ATTACTGCAG ATTCACTCTC CGGGGATTCA 
AAATTAATAC TTGAAGAGAT TACACAGCAC TTAAACTTGG AAAAACGTCT GCAGACCTAC 
AAATATCCTA TAACAATTAC CTGTTTAAAA GCTATCAGAA AATTACAAAA ATTTGGCCAT 
TTACCCAATA ACCCTAATAT TTTTCGTTCT TACTCATCAT ACGGAAATTT TATTGGAGTA 
AGACTTTGTG CATTAGAAGC TTTAGTTGAT TTCACTTGCT TAGATGGGCG TTGGGATGAT 
TTAAAGTATC TTATAGACAT AGCAGATAGT GATCCAGATC CAAAAGTTAG GAATGAACTT 
GTTCGCATGT TGTGTAAAAA TCCACCATTT ACACCTAAAA AACCAAATAA TCGATTAAAC 
AATGATTATC TATCTCATCA TTTATGGAAA CTTATCAATT CATTGTTATC TTATGATACT 
ACATTACGGT GTAATTTTGT TGACTTATAT TATACTTTAT TTGGACGTCA AAAAATTTTA 
CGTGCTGAAG TTGCGGCATT GTACAAGCCA ATCAAAGTTG AAGTAGAGGC AAAATCTTGT 
ACGCTTCCAA ATATTATTGT TGAACCAGAT AATAATTCTA ATAGTGAGCG TGGTATCAAA 
CGACTTATTG ATGAAGGAGA ATTAGATGTT CCAGAAATTG TTGATCAGCT AAAAGTTGAA 
AGTGATAATT TAGAAAATGA ATTTGGTGAT CATTTACATT TTGATAAGAA AATTAAAGCT 
GAGGTCAAAA AAGAAAGTGA AGAGTTAATG AATGAACTTC AAGAAGTCAA CTTAGTTTCA 
AATATCAGGC CTTCATTTGA ATTGAATGAA AACAAGTCTA AGACAGGTTA TTTCTCTGAA 
AATTCTGCTT CACTTCCTGG AATTATGATA TCCAATTCTA CTGGACCGGT TGGTTTTGAA 
CCCGGAATGT TTAATGTAAA ACCTGAAGAT CAGTCCAATA AACCTGTAGT TGTACAAAGT 
GAAAATAAAC ACCACCATCA TCACCATCAT CACCATCATC ATCATCATAG TAAAATAGAT 
GGAACCAGTA ATTCTAGTAA GAAAAAGAAG AAAGACAAAA AAAAACATAA GCACAAGCAT 
AAACACAAAC ATGAACACAA GCATAAGCAA GATAAATTGA AGGAAAAAAG TAAAGAAGAT 
AATACAATTA TTAAAGTAAA AGAAGAAACA TTGACATCTG GAAGTTCTAG TAGTGATGAT 
ACATCTAGAG ACTTGAGTGT ATTTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003243802.1
CDS	444..4193
Translation	MKKDKTGDTARPYKLAHQLVSLTGISFQRKNILGMVELTIIPLKNNLQFIKLNAKQCRIYRVTVNENIEVPFQYFDPFVDITQNKDEEPTLTAFSAAHLNAAQITEPDNNNGELVISLPAEITTCVSDGTSIKISVEYSLEQPSGGVHFVIPEGDGSLVERRAHMFTMCFENSSRLWFPCVDSYAEPCTWDLEFTVDENMTAVSCGDLIEVVAAPDLRRKTYYYSLSMPTCAPNIALAVGPFEIYVDPTMHIITHFCLPGLSELLPTAAKYTHEALEFYEDTLSNGYPYSCYKQVFVDETPTDYASYASMGIMSVNLLHPKQIIDQVYISKKILAQVMAEQFFGCFISTQNWSDWWLTCGIALYMSGLFAKKCFGNNSYREWIHNELTSLVRYEEEIGPIVLDPTQQPAPTSSGLVSPGGTSNPGFSLSFNNVQNTNETSNFYFSTRSLHTISPRYFHYMTKKAHLVLRMLENRIGYSLLLQVLNKHLSLASNASQNPMNSGLWGHMLISTNIFNKTIFTVSGIDMSVFIDQWVRTGGHAKFTCTFVFNRKRNTLELEIKQDAVCQRGIRKYVGPLLVSIQELDGPFKHTLQVEGNVARADITCHSKSRRNKKKKIPLCTGDEVDIDLNAMDNDSPVLWIRLDPEMTILRSCIIEQPEYQWNYQLRHEKDVTAQMEAVVALDKFPTIATRNALCDIIENEQVYIHVRCKAAHCLTKIANAMVTNWTGPPAMLATFKKMFGSFSCPHIIKQNNFSNLQHYYLQKTIPIAMAGLRNSHGICPPEILKFILDLFKYNDNSKNRYSDNYYRASLIDALGATITPVISLMQKACDITADSLSGDSKLILEEITQHLNLEKRLQTYKYPITITCLKAIRKLQKFGHLPNNPNIFRSYSSYGNFIGVRLCALEALVDFTCLDGRWDDLKYLIDIADSDPDPKVRNELVRMLCKNPPFTPKKPNNRLNNDYLSHHLWKLINSLLSYDTTLRCNFVDLYYTLFGRQKILRAEVAALYKPIKVEVEAKSCTLPNIIVEPDNNSNSERGIKRLIDEGELDVPEIVDQLKVESDNLENEFGDHLHFDKKIKAEVKKESEELMNELQEVNLVSNIRPSFELNENKSKTGYFSENSASLPGIMISNSTGPVGFEPGMFNVKPEDQSNKPVVVQSENKHHHHHHHHHHHHHSKIDGTSNSSKKKKKDKKKHKHKHKHKHEHKHKQDKLKEKSKEDNTIIKVKEETLTSGSSSSDDTSRDLSVFI

Target ORF information:

RefSeq Version	XM_003243754.4
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum transcription initiation factor TFIID subunit 2 (LOC100161509), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003243754.4

ORF Insert Sequence:

ATGAAGAAGG ATAAAACAGG AGACACCGCA AGGCCTTATA AACTAGCCCA TCAATTGGTT 
AGTTTGACAG GCATTAGTTT TCAACGAAAA AATATATTGG GAATGGTAGA GCTAACAATT 
ATACCATTGA AAAATAATCT TCAATTTATA AAACTAAATG CAAAACAATG TCGTATATAT 
CGCGTAACTG TTAATGAAAA CATTGAAGTT CCTTTTCAAT ATTTTGATCC ATTTGTTGAC 
ATTACTCAAA ATAAAGATGA AGAACCTACA TTAACTGCAT TTTCTGCAGC TCATTTAAAT 
GCTGCTCAGA TAACTGAACC CGATAACAAT AATGGGGAAT TAGTTATAAG TCTACCTGCA 
GAAATTACAA CATGTGTGAG TGATGGAACA AGTATTAAAA TAAGTGTAGA ATATTCTTTG 
GAACAGCCTA GTGGAGGTGT CCATTTTGTT ATTCCAGAAG GAGATGGATC TTTAGTTGAG 
AGACGAGCTC ATATGTTTAC TATGTGTTTT GAAAACTCAT CTCGACTATG GTTTCCGTGT 
GTCGATAGTT ATGCGGAACC ATGTACATGG GATTTAGAAT TTACAGTTGA TGAAAATATG 
ACGGCAGTAT CTTGTGGAGA TTTAATTGAA GTTGTTGCAG CTCCTGATTT AAGGCGTAAA 
ACATATTATT ATAGTTTATC AATGCCCACT TGTGCACCAA ATATAGCCTT AGCAGTGGGA 
CCTTTTGAAA TTTATGTGGA CCCTACGATG CATATTATAA CTCACTTCTG TTTGCCGGGG 
CTTTCTGAAC TTCTACCTAC AGCTGCAAAG TACACCCATG AAGCATTAGA ATTTTATGAA 
GATACGTTAT CTAACGGTTA TCCTTATAGT TGTTATAAAC AAGTATTTGT TGATGAAACT 
CCTACAGATT ATGCATCGTA TGCATCAATG GGTATTATGA GTGTTAATCT TCTACATCCA 
AAACAAATTA TTGATCAAGT ATATATTTCT AAAAAAATCT TGGCACAAGT TATGGCAGAG 
CAATTTTTCG GTTGTTTTAT TTCTACTCAA AATTGGTCTG ATTGGTGGCT AACGTGCGGT 
ATAGCATTAT ATATGTCTGG TCTTTTTGCA AAAAAATGTT TTGGCAATAA TTCTTATCGT 
GAATGGATTC ACAATGAATT AACAAGTTTG GTACGATATG AAGAAGAAAT AGGACCTATA 
GTGTTAGATC CAACCCAACA ACCTGCTCCA ACAAGTAGTG GTCTTGTCAG TCCAGGAGGA 
ACTTCTAATC CTGGATTTTC ATTATCTTTT AATAATGTGC AAAATACTAA TGAAACCTCA 
AATTTTTATT TCTCTACACG TAGCTTACAC ACAATATCAC CTCGTTATTT TCATTACATG 
ACTAAAAAGG CACATCTTGT ATTAAGAATG TTAGAAAATC GAATTGGCTA TTCATTATTG 
TTGCAGGTTT TGAACAAGCA TTTATCTTTA GCAAGCAATG CATCTCAAAA TCCAATGAAC 
TCAGGTTTAT GGGGCCACAT GTTAATTAGT ACAAATATAT TTAACAAGAC TATTTTTACT 
GTATCAGGCA TAGATATGTC TGTATTTATT GATCAATGGG TTAGAACTGG TGGTCACGCT 
AAATTTACTT GTACCTTTGT GTTCAATAGA AAAAGGAATA CATTGGAATT AGAAATTAAA 
CAAGATGCTG TTTGTCAACG AGGTATTCGT AAATATGTTG GCCCATTATT AGTTAGCATT 
CAAGAATTGG ATGGTCCATT TAAACATACT TTGCAAGTTG AAGGTAATGT AGCTCGAGCA 
GATATTACCT GTCATTCAAA AAGTAGGCGT AATAAGAAAA AGAAAATTCC TTTGTGTACG 
GGGGATGAAG TAGATATTGA TTTAAATGCT ATGGATAATG ACTCACCAGT ATTATGGATT 
CGATTAGATC CTGAGATGAC TATTTTACGC AGTTGTATTA TTGAACAACC TGAGTACCAG 
TGGAATTACC AATTGCGACA TGAAAAAGAT GTAACTGCCC AAATGGAAGC AGTGGTAGCT 
CTTGATAAAT TTCCTACAAT TGCTACACGT AATGCCTTAT GTGATATTAT TGAAAACGAA 
CAAGTGTATA TTCATGTACG GTGTAAAGCT GCTCACTGTT TAACTAAGAT TGCAAATGCA 
ATGGTTACTA ATTGGACTGG GCCTCCAGCA ATGTTGGCAA CATTTAAAAA AATGTTTGGT 
TCATTTTCAT GTCCACATAT AATAAAACAA AATAATTTCT CAAATCTACA ACATTACTAT 
CTACAGAAGA CAATTCCAAT TGCAATGGCT GGTTTGAGAA ATTCTCATGG AATTTGTCCC 
CCTGAAATCT TGAAATTCAT ATTAGATCTA TTCAAGTACA ATGATAATAG TAAAAATAGA 
TATTCTGATA ATTATTATAG AGCTTCACTG ATTGATGCTC TTGGTGCAAC AATTACACCA 
GTTATATCTT TAATGCAAAA AGCCTGTGAT ATTACTGCAG ATTCACTCTC CGGGGATTCA 
AAATTAATAC TTGAAGAGAT TACACAGCAC TTAAACTTGG AAAAACGTCT GCAGACCTAC 
AAATATCCTA TAACAATTAC CTGTTTAAAA GCTATCAGAA AATTACAAAA ATTTGGCCAT 
TTACCCAATA ACCCTAATAT TTTTCGTTCT TACTCATCAT ACGGAAATTT TATTGGAGTA 
AGACTTTGTG CATTAGAAGC TTTAGTTGAT TTCACTTGCT TAGATGGGCG TTGGGATGAT 
TTAAAGTATC TTATAGACAT AGCAGATAGT GATCCAGATC CAAAAGTTAG GAATGAACTT 
GTTCGCATGT TGTGTAAAAA TCCACCATTT ACACCTAAAA AACCAAATAA TCGATTAAAC 
AATGATTATC TATCTCATCA TTTATGGAAA CTTATCAATT CATTGTTATC TTATGATACT 
ACATTACGGT GTAATTTTGT TGACTTATAT TATACTTTAT TTGGACGTCA AAAAATTTTA 
CGTGCTGAAG TTGCGGCATT GTACAAGCCA ATCAAAGTTG AAGTAGAGGC AAAATCTTGT 
ACGCTTCCAA ATATTATTGT TGAACCAGAT AATAATTCTA ATAGTGAGCG TGGTATCAAA 
CGACTTATTG ATGAAGGAGA ATTAGATGTT CCAGAAATTG TTGATCAGCT AAAAGTTGAA 
AGTGATAATT TAGAAAATGA ATTTGGTGAT CATTTACATT TTGATAAGAA AATTAAAGCT 
GAGGTCAAAA AAGAAAGTGA AGAGTTAATG AATGAACTTC AAGAAGTCAA CTTAGTTTCA 
AATATCAGGC CTTCATTTGA ATTGAATGAA AACAAGTCTA AGACAGGTTA TTTCTCTGAA 
AATTCTGCTT CACTTCCTGG AATTATGATA TCCAATTCTA CTGGACCGGT TGGTTTTGAA 
CCCGGAATGT TTAATGTAAA ACCTGAAGAT CAGTCCAATA AACCTGTAGT TGTACAAAGT 
GAAAATAAAC ACCACCATCA TCACCATCAT CACCATCATC ATCATCATAG TAAAATAGAT 
GGAACCAGTA ATTCTAGTAA GAAAAAGAAG AAAGACAAAA AAAAACATAA GCACAAGCAT 
AAACACAAAC ATGAACACAA GCATAAGCAA GATAAATTGA AGGAAAAAAG TAAAGAAGAT 
AATACAATTA TTAAAGTAAA AGAAGAAACA TTGACATCTG GAAGTTCTAG TAGTGATGAT 
ACATCTAGAG ACTTGAGTGT ATTTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100161509 ( XM_003243754.3 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_003243802.1 Acyrthosiphon pisum transcription initiation factor TFIID subunit 2 (LOC100161509), mRNA.

Price & Availability↑

CloneID	OAd07905
Clone ID Related Accession (Same CDS sequence)	XM_003243754.4 , XM_003243754.3
Accession Version	XM_003243754.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463328000000
Organism	Acyrthosiphon pisum(pea aphid)
Product	transcription initiation factor TFIID subunit 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003383707.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2016 this sequence version replaced XM_003243754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acyrthosiphon pisum Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGG ATAAAACAGG AGACACCGCA AGGCCTTATA AACTAGCCCA TCAATTGGTT 
AGTTTGACAG GCATTAGTTT TCAACGAAAA AATATATTGG GAATGGTAGA GCTAACAATT 
ATACCATTGA AAAATAATCT TCAATTTATA AAACTAAATG CAAAACAATG TCGTATATAT 
CGCGTAACTG TTAATGAAAA CATTGAAGTT CCTTTTCAAT ATTTTGATCC ATTTGTTGAC 
ATTACTCAAA ATAAAGATGA AGAACCTACA TTAACTGCAT TTTCTGCAGC TCATTTAAAT 
GCTGCTCAGA TAACTGAACC CGATAACAAT AATGGGGAAT TAGTTATAAG TCTACCTGCA 
GAAATTACAA CATGTGTGAG TGATGGAACA AGTATTAAAA TAAGTGTAGA ATATTCTTTG 
GAACAGCCTA GTGGAGGTGT CCATTTTGTT ATTCCAGAAG GAGATGGATC TTTAGTTGAG 
AGACGAGCTC ATATGTTTAC TATGTGTTTT GAAAACTCAT CTCGACTATG GTTTCCGTGT 
GTCGATAGTT ATGCGGAACC ATGTACATGG GATTTAGAAT TTACAGTTGA TGAAAATATG 
ACGGCAGTAT CTTGTGGAGA TTTAATTGAA GTTGTTGCAG CTCCTGATTT AAGGCGTAAA 
ACATATTATT ATAGTTTATC AATGCCCACT TGTGCACCAA ATATAGCCTT AGCAGTGGGA 
CCTTTTGAAA TTTATGTGGA CCCTACGATG CATATTATAA CTCACTTCTG TTTGCCGGGG 
CTTTCTGAAC TTCTACCTAC AGCTGCAAAG TACACCCATG AAGCATTAGA ATTTTATGAA 
GATACGTTAT CTAACGGTTA TCCTTATAGT TGTTATAAAC AAGTATTTGT TGATGAAACT 
CCTACAGATT ATGCATCGTA TGCATCAATG GGTATTATGA GTGTTAATCT TCTACATCCA 
AAACAAATTA TTGATCAAGT ATATATTTCT AAAAAAATCT TGGCACAAGT TATGGCAGAG 
CAATTTTTCG GTTGTTTTAT TTCTACTCAA AATTGGTCTG ATTGGTGGCT AACGTGCGGT 
ATAGCATTAT ATATGTCTGG TCTTTTTGCA AAAAAATGTT TTGGCAATAA TTCTTATCGT 
GAATGGATTC ACAATGAATT AACAAGTTTG GTACGATATG AAGAAGAAAT AGGACCTATA 
GTGTTAGATC CAACCCAACA ACCTGCTCCA ACAAGTAGTG GTCTTGTCAG TCCAGGAGGA 
ACTTCTAATC CTGGATTTTC ATTATCTTTT AATAATGTGC AAAATACTAA TGAAACCTCA 
AATTTTTATT TCTCTACACG TAGCTTACAC ACAATATCAC CTCGTTATTT TCATTACATG 
ACTAAAAAGG CACATCTTGT ATTAAGAATG TTAGAAAATC GAATTGGCTA TTCATTATTG 
TTGCAGGTTT TGAACAAGCA TTTATCTTTA GCAAGCAATG CATCTCAAAA TCCAATGAAC 
TCAGGTTTAT GGGGCCACAT GTTAATTAGT ACAAATATAT TTAACAAGAC TATTTTTACT 
GTATCAGGCA TAGATATGTC TGTATTTATT GATCAATGGG TTAGAACTGG TGGTCACGCT 
AAATTTACTT GTACCTTTGT GTTCAATAGA AAAAGGAATA CATTGGAATT AGAAATTAAA 
CAAGATGCTG TTTGTCAACG AGGTATTCGT AAATATGTTG GCCCATTATT AGTTAGCATT 
CAAGAATTGG ATGGTCCATT TAAACATACT TTGCAAGTTG AAGGTAATGT AGCTCGAGCA 
GATATTACCT GTCATTCAAA AAGTAGGCGT AATAAGAAAA AGAAAATTCC TTTGTGTACG 
GGGGATGAAG TAGATATTGA TTTAAATGCT ATGGATAATG ACTCACCAGT ATTATGGATT 
CGATTAGATC CTGAGATGAC TATTTTACGC AGTTGTATTA TTGAACAACC TGAGTACCAG 
TGGAATTACC AATTGCGACA TGAAAAAGAT GTAACTGCCC AAATGGAAGC AGTGGTAGCT 
CTTGATAAAT TTCCTACAAT TGCTACACGT AATGCCTTAT GTGATATTAT TGAAAACGAA 
CAAGTGTATA TTCATGTACG GTGTAAAGCT GCTCACTGTT TAACTAAGAT TGCAAATGCA 
ATGGTTACTA ATTGGACTGG GCCTCCAGCA ATGTTGGCAA CATTTAAAAA AATGTTTGGT 
TCATTTTCAT GTCCACATAT AATAAAACAA AATAATTTCT CAAATCTACA ACATTACTAT 
CTACAGAAGA CAATTCCAAT TGCAATGGCT GGTTTGAGAA ATTCTCATGG AATTTGTCCC 
CCTGAAATCT TGAAATTCAT ATTAGATCTA TTCAAGTACA ATGATAATAG TAAAAATAGA 
TATTCTGATA ATTATTATAG AGCTTCACTG ATTGATGCTC TTGGTGCAAC AATTACACCA 
GTTATATCTT TAATGCAAAA AGCCTGTGAT ATTACTGCAG ATTCACTCTC CGGGGATTCA 
AAATTAATAC TTGAAGAGAT TACACAGCAC TTAAACTTGG AAAAACGTCT GCAGACCTAC 
AAATATCCTA TAACAATTAC CTGTTTAAAA GCTATCAGAA AATTACAAAA ATTTGGCCAT 
TTACCCAATA ACCCTAATAT TTTTCGTTCT TACTCATCAT ACGGAAATTT TATTGGAGTA 
AGACTTTGTG CATTAGAAGC TTTAGTTGAT TTCACTTGCT TAGATGGGCG TTGGGATGAT 
TTAAAGTATC TTATAGACAT AGCAGATAGT GATCCAGATC CAAAAGTTAG GAATGAACTT 
GTTCGCATGT TGTGTAAAAA TCCACCATTT ACACCTAAAA AACCAAATAA TCGATTAAAC 
AATGATTATC TATCTCATCA TTTATGGAAA CTTATCAATT CATTGTTATC TTATGATACT 
ACATTACGGT GTAATTTTGT TGACTTATAT TATACTTTAT TTGGACGTCA AAAAATTTTA 
CGTGCTGAAG TTGCGGCATT GTACAAGCCA ATCAAAGTTG AAGTAGAGGC AAAATCTTGT 
ACGCTTCCAA ATATTATTGT TGAACCAGAT AATAATTCTA ATAGTGAGCG TGGTATCAAA 
CGACTTATTG ATGAAGGAGA ATTAGATGTT CCAGAAATTG TTGATCAGCT AAAAGTTGAA 
AGTGATAATT TAGAAAATGA ATTTGGTGAT CATTTACATT TTGATAAGAA AATTAAAGCT 
GAGGTCAAAA AAGAAAGTGA AGAGTTAATG AATGAACTTC AAGAAGTCAA CTTAGTTTCA 
AATATCAGGC CTTCATTTGA ATTGAATGAA AACAAGTCTA AGACAGGTTA TTTCTCTGAA 
AATTCTGCTT CACTTCCTGG AATTATGATA TCCAATTCTA CTGGACCGGT TGGTTTTGAA 
CCCGGAATGT TTAATGTAAA ACCTGAAGAT CAGTCCAATA AACCTGTAGT TGTACAAAGT 
GAAAATAAAC ACCACCATCA TCACCATCAT CACCATCATC ATCATCATAG TAAAATAGAT 
GGAACCAGTA ATTCTAGTAA GAAAAAGAAG AAAGACAAAA AAAAACATAA GCACAAGCAT 
AAACACAAAC ATGAACACAA GCATAAGCAA GATAAATTGA AGGAAAAAAG TAAAGAAGAT 
AATACAATTA TTAAAGTAAA AGAAGAAACA TTGACATCTG GAAGTTCTAG TAGTGATGAT 
ACATCTAGAG ACTTGAGTGT ATTTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003243802.1
CDS	444..4193
Translation	MKKDKTGDTARPYKLAHQLVSLTGISFQRKNILGMVELTIIPLKNNLQFIKLNAKQCRIYRVTVNENIEVPFQYFDPFVDITQNKDEEPTLTAFSAAHLNAAQITEPDNNNGELVISLPAEITTCVSDGTSIKISVEYSLEQPSGGVHFVIPEGDGSLVERRAHMFTMCFENSSRLWFPCVDSYAEPCTWDLEFTVDENMTAVSCGDLIEVVAAPDLRRKTYYYSLSMPTCAPNIALAVGPFEIYVDPTMHIITHFCLPGLSELLPTAAKYTHEALEFYEDTLSNGYPYSCYKQVFVDETPTDYASYASMGIMSVNLLHPKQIIDQVYISKKILAQVMAEQFFGCFISTQNWSDWWLTCGIALYMSGLFAKKCFGNNSYREWIHNELTSLVRYEEEIGPIVLDPTQQPAPTSSGLVSPGGTSNPGFSLSFNNVQNTNETSNFYFSTRSLHTISPRYFHYMTKKAHLVLRMLENRIGYSLLLQVLNKHLSLASNASQNPMNSGLWGHMLISTNIFNKTIFTVSGIDMSVFIDQWVRTGGHAKFTCTFVFNRKRNTLELEIKQDAVCQRGIRKYVGPLLVSIQELDGPFKHTLQVEGNVARADITCHSKSRRNKKKKIPLCTGDEVDIDLNAMDNDSPVLWIRLDPEMTILRSCIIEQPEYQWNYQLRHEKDVTAQMEAVVALDKFPTIATRNALCDIIENEQVYIHVRCKAAHCLTKIANAMVTNWTGPPAMLATFKKMFGSFSCPHIIKQNNFSNLQHYYLQKTIPIAMAGLRNSHGICPPEILKFILDLFKYNDNSKNRYSDNYYRASLIDALGATITPVISLMQKACDITADSLSGDSKLILEEITQHLNLEKRLQTYKYPITITCLKAIRKLQKFGHLPNNPNIFRSYSSYGNFIGVRLCALEALVDFTCLDGRWDDLKYLIDIADSDPDPKVRNELVRMLCKNPPFTPKKPNNRLNNDYLSHHLWKLINSLLSYDTTLRCNFVDLYYTLFGRQKILRAEVAALYKPIKVEVEAKSCTLPNIIVEPDNNSNSERGIKRLIDEGELDVPEIVDQLKVESDNLENEFGDHLHFDKKIKAEVKKESEELMNELQEVNLVSNIRPSFELNENKSKTGYFSENSASLPGIMISNSTGPVGFEPGMFNVKPEDQSNKPVVVQSENKHHHHHHHHHHHHHSKIDGTSNSSKKKKKDKKKHKHKHKHKHEHKHKQDKLKEKSKEDNTIIKVKEETLTSGSSSSDDTSRDLSVFI

Target ORF information:

RefSeq Version	XM_003243754.3
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum transcription initiation factor TFIID subunit 2 (LOC100161509), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003243754.3

ORF Insert Sequence:

ATGAAGAAGG ATAAAACAGG AGACACCGCA AGGCCTTATA AACTAGCCCA TCAATTGGTT 
AGTTTGACAG GCATTAGTTT TCAACGAAAA AATATATTGG GAATGGTAGA GCTAACAATT 
ATACCATTGA AAAATAATCT TCAATTTATA AAACTAAATG CAAAACAATG TCGTATATAT 
CGCGTAACTG TTAATGAAAA CATTGAAGTT CCTTTTCAAT ATTTTGATCC ATTTGTTGAC 
ATTACTCAAA ATAAAGATGA AGAACCTACA TTAACTGCAT TTTCTGCAGC TCATTTAAAT 
GCTGCTCAGA TAACTGAACC CGATAACAAT AATGGGGAAT TAGTTATAAG TCTACCTGCA 
GAAATTACAA CATGTGTGAG TGATGGAACA AGTATTAAAA TAAGTGTAGA ATATTCTTTG 
GAACAGCCTA GTGGAGGTGT CCATTTTGTT ATTCCAGAAG GAGATGGATC TTTAGTTGAG 
AGACGAGCTC ATATGTTTAC TATGTGTTTT GAAAACTCAT CTCGACTATG GTTTCCGTGT 
GTCGATAGTT ATGCGGAACC ATGTACATGG GATTTAGAAT TTACAGTTGA TGAAAATATG 
ACGGCAGTAT CTTGTGGAGA TTTAATTGAA GTTGTTGCAG CTCCTGATTT AAGGCGTAAA 
ACATATTATT ATAGTTTATC AATGCCCACT TGTGCACCAA ATATAGCCTT AGCAGTGGGA 
CCTTTTGAAA TTTATGTGGA CCCTACGATG CATATTATAA CTCACTTCTG TTTGCCGGGG 
CTTTCTGAAC TTCTACCTAC AGCTGCAAAG TACACCCATG AAGCATTAGA ATTTTATGAA 
GATACGTTAT CTAACGGTTA TCCTTATAGT TGTTATAAAC AAGTATTTGT TGATGAAACT 
CCTACAGATT ATGCATCGTA TGCATCAATG GGTATTATGA GTGTTAATCT TCTACATCCA 
AAACAAATTA TTGATCAAGT ATATATTTCT AAAAAAATCT TGGCACAAGT TATGGCAGAG 
CAATTTTTCG GTTGTTTTAT TTCTACTCAA AATTGGTCTG ATTGGTGGCT AACGTGCGGT 
ATAGCATTAT ATATGTCTGG TCTTTTTGCA AAAAAATGTT TTGGCAATAA TTCTTATCGT 
GAATGGATTC ACAATGAATT AACAAGTTTG GTACGATATG AAGAAGAAAT AGGACCTATA 
GTGTTAGATC CAACCCAACA ACCTGCTCCA ACAAGTAGTG GTCTTGTCAG TCCAGGAGGA 
ACTTCTAATC CTGGATTTTC ATTATCTTTT AATAATGTGC AAAATACTAA TGAAACCTCA 
AATTTTTATT TCTCTACACG TAGCTTACAC ACAATATCAC CTCGTTATTT TCATTACATG 
ACTAAAAAGG CACATCTTGT ATTAAGAATG TTAGAAAATC GAATTGGCTA TTCATTATTG 
TTGCAGGTTT TGAACAAGCA TTTATCTTTA GCAAGCAATG CATCTCAAAA TCCAATGAAC 
TCAGGTTTAT GGGGCCACAT GTTAATTAGT ACAAATATAT TTAACAAGAC TATTTTTACT 
GTATCAGGCA TAGATATGTC TGTATTTATT GATCAATGGG TTAGAACTGG TGGTCACGCT 
AAATTTACTT GTACCTTTGT GTTCAATAGA AAAAGGAATA CATTGGAATT AGAAATTAAA 
CAAGATGCTG TTTGTCAACG AGGTATTCGT AAATATGTTG GCCCATTATT AGTTAGCATT 
CAAGAATTGG ATGGTCCATT TAAACATACT TTGCAAGTTG AAGGTAATGT AGCTCGAGCA 
GATATTACCT GTCATTCAAA AAGTAGGCGT AATAAGAAAA AGAAAATTCC TTTGTGTACG 
GGGGATGAAG TAGATATTGA TTTAAATGCT ATGGATAATG ACTCACCAGT ATTATGGATT 
CGATTAGATC CTGAGATGAC TATTTTACGC AGTTGTATTA TTGAACAACC TGAGTACCAG 
TGGAATTACC AATTGCGACA TGAAAAAGAT GTAACTGCCC AAATGGAAGC AGTGGTAGCT 
CTTGATAAAT TTCCTACAAT TGCTACACGT AATGCCTTAT GTGATATTAT TGAAAACGAA 
CAAGTGTATA TTCATGTACG GTGTAAAGCT GCTCACTGTT TAACTAAGAT TGCAAATGCA 
ATGGTTACTA ATTGGACTGG GCCTCCAGCA ATGTTGGCAA CATTTAAAAA AATGTTTGGT 
TCATTTTCAT GTCCACATAT AATAAAACAA AATAATTTCT CAAATCTACA ACATTACTAT 
CTACAGAAGA CAATTCCAAT TGCAATGGCT GGTTTGAGAA ATTCTCATGG AATTTGTCCC 
CCTGAAATCT TGAAATTCAT ATTAGATCTA TTCAAGTACA ATGATAATAG TAAAAATAGA 
TATTCTGATA ATTATTATAG AGCTTCACTG ATTGATGCTC TTGGTGCAAC AATTACACCA 
GTTATATCTT TAATGCAAAA AGCCTGTGAT ATTACTGCAG ATTCACTCTC CGGGGATTCA 
AAATTAATAC TTGAAGAGAT TACACAGCAC TTAAACTTGG AAAAACGTCT GCAGACCTAC 
AAATATCCTA TAACAATTAC CTGTTTAAAA GCTATCAGAA AATTACAAAA ATTTGGCCAT 
TTACCCAATA ACCCTAATAT TTTTCGTTCT TACTCATCAT ACGGAAATTT TATTGGAGTA 
AGACTTTGTG CATTAGAAGC TTTAGTTGAT TTCACTTGCT TAGATGGGCG TTGGGATGAT 
TTAAAGTATC TTATAGACAT AGCAGATAGT GATCCAGATC CAAAAGTTAG GAATGAACTT 
GTTCGCATGT TGTGTAAAAA TCCACCATTT ACACCTAAAA AACCAAATAA TCGATTAAAC 
AATGATTATC TATCTCATCA TTTATGGAAA CTTATCAATT CATTGTTATC TTATGATACT 
ACATTACGGT GTAATTTTGT TGACTTATAT TATACTTTAT TTGGACGTCA AAAAATTTTA 
CGTGCTGAAG TTGCGGCATT GTACAAGCCA ATCAAAGTTG AAGTAGAGGC AAAATCTTGT 
ACGCTTCCAA ATATTATTGT TGAACCAGAT AATAATTCTA ATAGTGAGCG TGGTATCAAA 
CGACTTATTG ATGAAGGAGA ATTAGATGTT CCAGAAATTG TTGATCAGCT AAAAGTTGAA 
AGTGATAATT TAGAAAATGA ATTTGGTGAT CATTTACATT TTGATAAGAA AATTAAAGCT 
GAGGTCAAAA AAGAAAGTGA AGAGTTAATG AATGAACTTC AAGAAGTCAA CTTAGTTTCA 
AATATCAGGC CTTCATTTGA ATTGAATGAA AACAAGTCTA AGACAGGTTA TTTCTCTGAA 
AATTCTGCTT CACTTCCTGG AATTATGATA TCCAATTCTA CTGGACCGGT TGGTTTTGAA 
CCCGGAATGT TTAATGTAAA ACCTGAAGAT CAGTCCAATA AACCTGTAGT TGTACAAAGT 
GAAAATAAAC ACCACCATCA TCACCATCAT CACCATCATC ATCATCATAG TAAAATAGAT 
GGAACCAGTA ATTCTAGTAA GAAAAAGAAG AAAGACAAAA AAAAACATAA GCACAAGCAT 
AAACACAAAC ATGAACACAA GCATAAGCAA GATAAATTGA AGGAAAAAAG TAAAGAAGAT 
AATACAATTA TTAAAGTAAA AGAAGAAACA TTGACATCTG GAAGTTCTAG TAGTGATGAT 
ACATCTAGAG ACTTGAGTGT ATTTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100161509 cDNA ORF clone, Acyrthosiphon pisum(pea aphid)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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