PLEKHH2 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLEKHH2
Entrez Gene ID	101114431
Full Name	pleckstrin homology, MyTH4 and FERM domain containing H2
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012172698.3	XP_012028088.2	pleckstrin homology domain-containing family H member 2
XM_012115010.2	XP_011970400.1	pleckstrin homology domain-containing family H member 2
XM_012172698.2	XP_012028088.1	pleckstrin homology domain-containing family H member 2
XM_012115009.2	XP_011970399.1	pleckstrin homology domain-containing family H member 2
XM_004005992.2	XP_004006041.1	pleckstrin homology domain-containing family H member 2
XM_012172689.2	XP_012028079.1	pleckstrin homology domain-containing family H member 2
XM_012115008.1	XP_011970398.1	pleckstrin homology domain-containing family H member 2
XM_004005992.3	XP_004006041.2	pleckstrin homology domain-containing family H member 2

Pathways from BioSystems
Gene Ontology

The following PLEKHH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv04151	XM_012115009.2 Latest version!	Ovis aries musimon pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OOv04151	XM_012115010.2 Latest version!	Ovis aries musimon pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OOv04151	XM_012115008.1 Latest version!	Ovis aries musimon pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSm03959	XM_004005992.2 Latest version!	Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSm03959	XM_012172689.2 Latest version!	Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSm03959	XM_012172698.2 Latest version!	Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSm77298	XM_004005992.3 Latest version!	Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSm77298	XM_012172698.3 Latest version!	Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLEKHH2 ( XM_012115010.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011970400.1 Ovis aries musimon pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOv04151
Clone ID Related Accession (Same CDS sequence)	XM_012115010.2 , XM_012115009.2 , XM_012115008.1
Accession Version	XM_012115010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	pleckstrin homology domain-containing family H member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942260.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012115010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAACTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GGCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTG TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCGC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCAGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011970400.1
CDS	191..4666
Translation	MAELSETEGPVDWKERCVALESQLMKFRVQASKIRELLAEKMQQLERQVIDAERQAEKAFQQVQVMEEKLKAANIQTSESETRLYKKCQDLETLIQEKEDIIQNLELQLEEQKQIRIQEAKIIEEKAAKIKEWVTVKLNELELENQNLRLINQNQTEEIKTIQSKLQEVQGKKSVVSTPKLSEGQRLSSLTFGCFLSRARSPPQVVKSEETSKISSKEPEFTEGRDIEEMEIPEKSVDNQIQENNRGQRTLHQGPCTSEQNRKTGTSFVMDGGTSQNSGAPGSDWSSDEEDGSKGRSKSRYTSTLSSHTSEDGVQSSRMGSETYLTASDDSSSIFEEETFGLKRPEHKKLYSWQQEAQWKAQSCPLGKGNSESRKKERDSSSDELNKKFQSQRLDYSSSSSEANTPSPILTPALSPKHPNPLLGKGAELGPSSTLPPPKLRIPNIFSLSIALAKRHLSQPQLSSDRMFGTNRNAISMIRPLRPQETDLDLVDGDNTEILETMDTNCDDGLFSYDCLESPCSEDQEACDSAKKAACGKPPTPPLHRFPSWESRIYAVAKSGIRMSEAFNMENVHKHSAAVLSYTTSGLYTSLIYKNMTTPVYTTLKGKATQISSSPFLDDSSGSEEEDSSRSSSRTSESDSRSRSGPGSPRALKRGVSLSSVASESDYAIPPDAYSADLEYSQPEQKLLKTCSSSSDNGKNEPLEKSGYLLKMSGKVKTWKRRWFVLKGGELLYYKSPSDVIRKPQGHIELSASCSILRGDNKQTVQLTTEKHTYYLTADSPNILEEWIKVLQNVLRVQAANPLFLQPEGKPTVKGLLTKVKHGYSKRVWCKLIGKTLYYFRSQEDKFPLGQIKLWEAKVEEVDRSCDSDEDYEASGRSLLSTHYTIVIHPKDQGPTYLLIGSKHEKDTWLYHLTVAAGSNNVNVGSEFEQLVCKLLNIEGEPSSQIWRHPTLCHSKEGIISPLTTLPSEALQTEAIKLFKTCQLFINAAVDSPAIDYHISLAQSALQICLTHPELQNEICCQLIKQTRWRQPQNQPGPLQGWQLLALCVGLFLPHHPFLWLLRLHLKRNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSLPFSIPVHFMNGIYQVVGFDASTTVEEFLNTLNQDTGMRKPAQSGFALFTDDPSGRDLEHCLQGNIKICDIISKWEQASKEQQPGKWEGTRTVRLTYKNRLYFSMQVRGETDREKLLLMYQTNDQIVNGLFPLNRDLALEMAALLAQVEIGDFERPFSTPAGHVTNQCKANQTLKQVIEKFYPKRYRDDSSEEQLRQLCQRLSTRWMALRGHSAADCVRIYLTVARKWPFFGAKLFLAKPITPSSLGSTFMWLAVHEDGLSILEYNSMRLIVSYVYKSLMTFGGYQDDFMVVINNAHSKEKPTEKLIFAMAKPKILEITLLIASYINNFYQQKAVHHLSAPALLSAQTQGFQARVMGSQPLLASSRPTKGPTLL

Target ORF information:

RefSeq Version	XM_012115010.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012115010.2

ORF Insert Sequence:

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAACTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GGCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTG TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCGC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCAGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHH2 ( XM_012115009.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011970399.1 Ovis aries musimon pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv04151
Clone ID Related Accession (Same CDS sequence)	XM_012115010.2 , XM_012115009.2 , XM_012115008.1
Accession Version	XM_012115009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	pleckstrin homology domain-containing family H member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942260.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012115009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAACTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GGCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTG TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCGC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCAGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011970399.1
CDS	172..4647
Translation	MAELSETEGPVDWKERCVALESQLMKFRVQASKIRELLAEKMQQLERQVIDAERQAEKAFQQVQVMEEKLKAANIQTSESETRLYKKCQDLETLIQEKEDIIQNLELQLEEQKQIRIQEAKIIEEKAAKIKEWVTVKLNELELENQNLRLINQNQTEEIKTIQSKLQEVQGKKSVVSTPKLSEGQRLSSLTFGCFLSRARSPPQVVKSEETSKISSKEPEFTEGRDIEEMEIPEKSVDNQIQENNRGQRTLHQGPCTSEQNRKTGTSFVMDGGTSQNSGAPGSDWSSDEEDGSKGRSKSRYTSTLSSHTSEDGVQSSRMGSETYLTASDDSSSIFEEETFGLKRPEHKKLYSWQQEAQWKAQSCPLGKGNSESRKKERDSSSDELNKKFQSQRLDYSSSSSEANTPSPILTPALSPKHPNPLLGKGAELGPSSTLPPPKLRIPNIFSLSIALAKRHLSQPQLSSDRMFGTNRNAISMIRPLRPQETDLDLVDGDNTEILETMDTNCDDGLFSYDCLESPCSEDQEACDSAKKAACGKPPTPPLHRFPSWESRIYAVAKSGIRMSEAFNMENVHKHSAAVLSYTTSGLYTSLIYKNMTTPVYTTLKGKATQISSSPFLDDSSGSEEEDSSRSSSRTSESDSRSRSGPGSPRALKRGVSLSSVASESDYAIPPDAYSADLEYSQPEQKLLKTCSSSSDNGKNEPLEKSGYLLKMSGKVKTWKRRWFVLKGGELLYYKSPSDVIRKPQGHIELSASCSILRGDNKQTVQLTTEKHTYYLTADSPNILEEWIKVLQNVLRVQAANPLFLQPEGKPTVKGLLTKVKHGYSKRVWCKLIGKTLYYFRSQEDKFPLGQIKLWEAKVEEVDRSCDSDEDYEASGRSLLSTHYTIVIHPKDQGPTYLLIGSKHEKDTWLYHLTVAAGSNNVNVGSEFEQLVCKLLNIEGEPSSQIWRHPTLCHSKEGIISPLTTLPSEALQTEAIKLFKTCQLFINAAVDSPAIDYHISLAQSALQICLTHPELQNEICCQLIKQTRWRQPQNQPGPLQGWQLLALCVGLFLPHHPFLWLLRLHLKRNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSLPFSIPVHFMNGIYQVVGFDASTTVEEFLNTLNQDTGMRKPAQSGFALFTDDPSGRDLEHCLQGNIKICDIISKWEQASKEQQPGKWEGTRTVRLTYKNRLYFSMQVRGETDREKLLLMYQTNDQIVNGLFPLNRDLALEMAALLAQVEIGDFERPFSTPAGHVTNQCKANQTLKQVIEKFYPKRYRDDSSEEQLRQLCQRLSTRWMALRGHSAADCVRIYLTVARKWPFFGAKLFLAKPITPSSLGSTFMWLAVHEDGLSILEYNSMRLIVSYVYKSLMTFGGYQDDFMVVINNAHSKEKPTEKLIFAMAKPKILEITLLIASYINNFYQQKAVHHLSAPALLSAQTQGFQARVMGSQPLLASSRPTKGPTLL

Target ORF information:

RefSeq Version	XM_012115009.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012115009.2

ORF Insert Sequence:

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAACTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GGCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTG TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCGC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCAGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHH2 ( XM_012115008.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011970398.1 Ovis aries musimon pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X1, mRNA.

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CloneID	OOv04151
Clone ID Related Accession (Same CDS sequence)	XM_012115010.2 , XM_012115009.2 , XM_012115008.1
Accession Version	XM_012115008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	pleckstrin homology domain-containing family H member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942260.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAACTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GGCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTG TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCGC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCAGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011970398.1
CDS	165..4640
Translation	MAELSETEGPVDWKERCVALESQLMKFRVQASKIRELLAEKMQQLERQVIDAERQAEKAFQQVQVMEEKLKAANIQTSESETRLYKKCQDLETLIQEKEDIIQNLELQLEEQKQIRIQEAKIIEEKAAKIKEWVTVKLNELELENQNLRLINQNQTEEIKTIQSKLQEVQGKKSVVSTPKLSEGQRLSSLTFGCFLSRARSPPQVVKSEETSKISSKEPEFTEGRDIEEMEIPEKSVDNQIQENNRGQRTLHQGPCTSEQNRKTGTSFVMDGGTSQNSGAPGSDWSSDEEDGSKGRSKSRYTSTLSSHTSEDGVQSSRMGSETYLTASDDSSSIFEEETFGLKRPEHKKLYSWQQEAQWKAQSCPLGKGNSESRKKERDSSSDELNKKFQSQRLDYSSSSSEANTPSPILTPALSPKHPNPLLGKGAELGPSSTLPPPKLRIPNIFSLSIALAKRHLSQPQLSSDRMFGTNRNAISMIRPLRPQETDLDLVDGDNTEILETMDTNCDDGLFSYDCLESPCSEDQEACDSAKKAACGKPPTPPLHRFPSWESRIYAVAKSGIRMSEAFNMENVHKHSAAVLSYTTSGLYTSLIYKNMTTPVYTTLKGKATQISSSPFLDDSSGSEEEDSSRSSSRTSESDSRSRSGPGSPRALKRGVSLSSVASESDYAIPPDAYSADLEYSQPEQKLLKTCSSSSDNGKNEPLEKSGYLLKMSGKVKTWKRRWFVLKGGELLYYKSPSDVIRKPQGHIELSASCSILRGDNKQTVQLTTEKHTYYLTADSPNILEEWIKVLQNVLRVQAANPLFLQPEGKPTVKGLLTKVKHGYSKRVWCKLIGKTLYYFRSQEDKFPLGQIKLWEAKVEEVDRSCDSDEDYEASGRSLLSTHYTIVIHPKDQGPTYLLIGSKHEKDTWLYHLTVAAGSNNVNVGSEFEQLVCKLLNIEGEPSSQIWRHPTLCHSKEGIISPLTTLPSEALQTEAIKLFKTCQLFINAAVDSPAIDYHISLAQSALQICLTHPELQNEICCQLIKQTRWRQPQNQPGPLQGWQLLALCVGLFLPHHPFLWLLRLHLKRNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSLPFSIPVHFMNGIYQVVGFDASTTVEEFLNTLNQDTGMRKPAQSGFALFTDDPSGRDLEHCLQGNIKICDIISKWEQASKEQQPGKWEGTRTVRLTYKNRLYFSMQVRGETDREKLLLMYQTNDQIVNGLFPLNRDLALEMAALLAQVEIGDFERPFSTPAGHVTNQCKANQTLKQVIEKFYPKRYRDDSSEEQLRQLCQRLSTRWMALRGHSAADCVRIYLTVARKWPFFGAKLFLAKPITPSSLGSTFMWLAVHEDGLSILEYNSMRLIVSYVYKSLMTFGGYQDDFMVVINNAHSKEKPTEKLIFAMAKPKILEITLLIASYINNFYQQKAVHHLSAPALLSAQTQGFQARVMGSQPLLASSRPTKGPTLL

Target ORF information:

RefSeq Version	XM_012115008.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012115008.1

ORF Insert Sequence:

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAACTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GGCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTG TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCGC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCAGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHH2 ( XM_012172698.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012028088.1 Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X3, mRNA.

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CloneID	OSm03959
Clone ID Related Accession (Same CDS sequence)	XM_012172698.2 , XM_004005992.2 , XM_012172689.2
Accession Version	XM_012172698.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	pleckstrin homology domain-containing family H member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012172698.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAGCTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GCCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTA TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCAC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCGGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012028088.1
CDS	199..4674
Translation	MAELSETEGPVDWKERCVALESQLMKFRVQASKIRELLAEKMQQLERQVIDAERQAEKAFQQVQVMEEKLKAANIQTSESETRLYKKCQDLETLIQEKEDIIQNLELQLEEQKQIRIQEAKIIEEKAAKIKEWVTVKLNELELENQNLRLINQNQTEEIKTIQSKLQEVQGKKSVVSTPKLSEGQRLSSLTFGCFLSRARSPPQVVKSEETSKISSKEPEFTEGRDIEEMEIPEKSVDNQIQENNRGQRTLHQGPCTSEQNRKTGTSFVMDGGTSQNSGAPGSDWSSDEEDGSKGRSKSRYTSTLSSHTSEDGVQSSRMGSETYLTASDDSSSIFEEETFGLKRPEHKKLYSWQQEAQWKAQSCPLGKGNSESRKKERDSSSDELNKKFQSQRLDYSSSSSEANTPSPILTPALSPKHPNPLLGKGTELGPSSTLPPPKLRIPNIFSLSIALAKRHLSQPQLSSDRMFGTNRNAISMIRPLRPQETDLDLVDGDNTEILETMDTNCDDGLFSYDCLESPCSEDQEACDSAKKAACGKPPTPPLHRFPSWESRIYAVAKSGIRMSEAFNMENVHKHSAAVLSYTTSGLYTSLIYKNMTTPVYTTLKGKATQISSSPFLDDSSGSEEEDSSRSSSRTSESDSRSRSGPGSPRALKRGVSLSSVASESDYAIPPDAYSADLEYSQPEQKLLKTCSSSSDNGKNEPLEKSGYLLKMSGKVKTWKRRWFVLKGGELLYYKSPSDVIRKPQGHIELSASCSILRGDNKQTVQLTTEKHTYYLTADSPNILEEWIKVLQNVLRVQAANPLFLQPEGKPTVKGLLTKVKHGYSKRVWCKLIGKTLYYFRSQEDKFPLGQIKLWEAKVEEVDRSCDSDEDYEASGRSLLSTHYTIVIHPKDQGPTYLLIGSKHEKDTWLYHLTVAAGSNNVNVGSEFEQLVCKLLNIEGEPSSQIWRHPTLCHSKEGIISPLTTLPSEALQTEAIKLFKTCQLFINAAVDSPAIDYHISLAQSALQICLTHPELQNEICCQLIKQTRWRQPQNQPGPLQGWQLLALCVGLFLPHHPFLWLLRLHLKRNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSLPFSIPVHFMNGIYQVVGFDASTTVEEFLNTLNQDTGMRKPAQSGFALFTDDPSGRDLEHCLQGNIKICDIISKWEQASKEQQPGKWEGTRTVRLTYKNRLYFSMQVRGETDREKLLLMYQTNDQIVNGLFPLNRDLALEMAALLAQVEIGDFERPFSTPAGHVTNQCKANQTLKQVIEKFYPKRYRDDSSEEQLRQLCQRLSTRWMALRGHSAADCVRIYLTVARKWPFFGAKLFLAKPITPSSLGSTFMWLAVHEDGLSILEYNSMRLIVSYVYKSLMTFGGYQDDFMVVINNAHSKEKPTEKLIFAMAKPKILEITLLIASYINNFYQQKAVHHLSAPALLSAQTQGFQARVMGSQPLLASSRPTKGPTLL

Target ORF information:

RefSeq Version	XM_012172698.2
Organism	Ovis aries(sheep)
Definition	Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012172698.2

ORF Insert Sequence:

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAGCTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GCCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTA TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCAC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCGGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHH2 ( XM_004005992.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004006041.1 Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X1, mRNA.

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CloneID	OSm03959
Clone ID Related Accession (Same CDS sequence)	XM_012172698.2 , XM_004005992.2 , XM_012172689.2
Accession Version	XM_004005992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	pleckstrin homology domain-containing family H member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 3, 2015 this sequence version replaced XM_004005992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAGCTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GCCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTA TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCAC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCGGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004006041.1
CDS	146..4621
Translation	MAELSETEGPVDWKERCVALESQLMKFRVQASKIRELLAEKMQQLERQVIDAERQAEKAFQQVQVMEEKLKAANIQTSESETRLYKKCQDLETLIQEKEDIIQNLELQLEEQKQIRIQEAKIIEEKAAKIKEWVTVKLNELELENQNLRLINQNQTEEIKTIQSKLQEVQGKKSVVSTPKLSEGQRLSSLTFGCFLSRARSPPQVVKSEETSKISSKEPEFTEGRDIEEMEIPEKSVDNQIQENNRGQRTLHQGPCTSEQNRKTGTSFVMDGGTSQNSGAPGSDWSSDEEDGSKGRSKSRYTSTLSSHTSEDGVQSSRMGSETYLTASDDSSSIFEEETFGLKRPEHKKLYSWQQEAQWKAQSCPLGKGNSESRKKERDSSSDELNKKFQSQRLDYSSSSSEANTPSPILTPALSPKHPNPLLGKGTELGPSSTLPPPKLRIPNIFSLSIALAKRHLSQPQLSSDRMFGTNRNAISMIRPLRPQETDLDLVDGDNTEILETMDTNCDDGLFSYDCLESPCSEDQEACDSAKKAACGKPPTPPLHRFPSWESRIYAVAKSGIRMSEAFNMENVHKHSAAVLSYTTSGLYTSLIYKNMTTPVYTTLKGKATQISSSPFLDDSSGSEEEDSSRSSSRTSESDSRSRSGPGSPRALKRGVSLSSVASESDYAIPPDAYSADLEYSQPEQKLLKTCSSSSDNGKNEPLEKSGYLLKMSGKVKTWKRRWFVLKGGELLYYKSPSDVIRKPQGHIELSASCSILRGDNKQTVQLTTEKHTYYLTADSPNILEEWIKVLQNVLRVQAANPLFLQPEGKPTVKGLLTKVKHGYSKRVWCKLIGKTLYYFRSQEDKFPLGQIKLWEAKVEEVDRSCDSDEDYEASGRSLLSTHYTIVIHPKDQGPTYLLIGSKHEKDTWLYHLTVAAGSNNVNVGSEFEQLVCKLLNIEGEPSSQIWRHPTLCHSKEGIISPLTTLPSEALQTEAIKLFKTCQLFINAAVDSPAIDYHISLAQSALQICLTHPELQNEICCQLIKQTRWRQPQNQPGPLQGWQLLALCVGLFLPHHPFLWLLRLHLKRNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSLPFSIPVHFMNGIYQVVGFDASTTVEEFLNTLNQDTGMRKPAQSGFALFTDDPSGRDLEHCLQGNIKICDIISKWEQASKEQQPGKWEGTRTVRLTYKNRLYFSMQVRGETDREKLLLMYQTNDQIVNGLFPLNRDLALEMAALLAQVEIGDFERPFSTPAGHVTNQCKANQTLKQVIEKFYPKRYRDDSSEEQLRQLCQRLSTRWMALRGHSAADCVRIYLTVARKWPFFGAKLFLAKPITPSSLGSTFMWLAVHEDGLSILEYNSMRLIVSYVYKSLMTFGGYQDDFMVVINNAHSKEKPTEKLIFAMAKPKILEITLLIASYINNFYQQKAVHHLSAPALLSAQTQGFQARVMGSQPLLASSRPTKGPTLL

Target ORF information:

RefSeq Version	XM_004005992.2
Organism	Ovis aries(sheep)
Definition	Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004005992.2

ORF Insert Sequence:

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAGCTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GCCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTA TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCAC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCGGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHH2 ( XM_012172689.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012028079.1 Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X2, mRNA.

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CloneID	OSm03959
Clone ID Related Accession (Same CDS sequence)	XM_012172698.2 , XM_004005992.2 , XM_012172689.2
Accession Version	XM_012172689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	pleckstrin homology domain-containing family H member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012172689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAGCTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GCCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTA TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCAC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCGGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012028079.1
CDS	181..4656
Translation	MAELSETEGPVDWKERCVALESQLMKFRVQASKIRELLAEKMQQLERQVIDAERQAEKAFQQVQVMEEKLKAANIQTSESETRLYKKCQDLETLIQEKEDIIQNLELQLEEQKQIRIQEAKIIEEKAAKIKEWVTVKLNELELENQNLRLINQNQTEEIKTIQSKLQEVQGKKSVVSTPKLSEGQRLSSLTFGCFLSRARSPPQVVKSEETSKISSKEPEFTEGRDIEEMEIPEKSVDNQIQENNRGQRTLHQGPCTSEQNRKTGTSFVMDGGTSQNSGAPGSDWSSDEEDGSKGRSKSRYTSTLSSHTSEDGVQSSRMGSETYLTASDDSSSIFEEETFGLKRPEHKKLYSWQQEAQWKAQSCPLGKGNSESRKKERDSSSDELNKKFQSQRLDYSSSSSEANTPSPILTPALSPKHPNPLLGKGTELGPSSTLPPPKLRIPNIFSLSIALAKRHLSQPQLSSDRMFGTNRNAISMIRPLRPQETDLDLVDGDNTEILETMDTNCDDGLFSYDCLESPCSEDQEACDSAKKAACGKPPTPPLHRFPSWESRIYAVAKSGIRMSEAFNMENVHKHSAAVLSYTTSGLYTSLIYKNMTTPVYTTLKGKATQISSSPFLDDSSGSEEEDSSRSSSRTSESDSRSRSGPGSPRALKRGVSLSSVASESDYAIPPDAYSADLEYSQPEQKLLKTCSSSSDNGKNEPLEKSGYLLKMSGKVKTWKRRWFVLKGGELLYYKSPSDVIRKPQGHIELSASCSILRGDNKQTVQLTTEKHTYYLTADSPNILEEWIKVLQNVLRVQAANPLFLQPEGKPTVKGLLTKVKHGYSKRVWCKLIGKTLYYFRSQEDKFPLGQIKLWEAKVEEVDRSCDSDEDYEASGRSLLSTHYTIVIHPKDQGPTYLLIGSKHEKDTWLYHLTVAAGSNNVNVGSEFEQLVCKLLNIEGEPSSQIWRHPTLCHSKEGIISPLTTLPSEALQTEAIKLFKTCQLFINAAVDSPAIDYHISLAQSALQICLTHPELQNEICCQLIKQTRWRQPQNQPGPLQGWQLLALCVGLFLPHHPFLWLLRLHLKRNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSLPFSIPVHFMNGIYQVVGFDASTTVEEFLNTLNQDTGMRKPAQSGFALFTDDPSGRDLEHCLQGNIKICDIISKWEQASKEQQPGKWEGTRTVRLTYKNRLYFSMQVRGETDREKLLLMYQTNDQIVNGLFPLNRDLALEMAALLAQVEIGDFERPFSTPAGHVTNQCKANQTLKQVIEKFYPKRYRDDSSEEQLRQLCQRLSTRWMALRGHSAADCVRIYLTVARKWPFFGAKLFLAKPITPSSLGSTFMWLAVHEDGLSILEYNSMRLIVSYVYKSLMTFGGYQDDFMVVINNAHSKEKPTEKLIFAMAKPKILEITLLIASYINNFYQQKAVHHLSAPALLSAQTQGFQARVMGSQPLLASSRPTKGPTLL

Target ORF information:

RefSeq Version	XM_012172689.2
Organism	Ovis aries(sheep)
Definition	Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012172689.2

ORF Insert Sequence:

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAGCTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GCCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTA TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCAC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTCGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GTCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCGGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA AATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC GAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGCGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAACCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHH2 ( XM_012172698.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012028088.2 Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSm77298
Clone ID Related Accession (Same CDS sequence)	XM_012172698.3 , XM_004005992.3
Accession Version	XM_012172698.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	pleckstrin homology domain-containing family H member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012172698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAGCTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GCCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTG TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCAC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTTGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GCCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCGGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA GATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC CAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGTGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAATCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012028088.2
CDS	181..4656
Translation	MAELSETEGPVDWKERCVALESQLMKFRVQASKIRELLAEKMQQLERQVIDAERQAEKAFQQVQVMEEKLKAANIQTSESETRLYKKCQDLETLIQEKEDIIQNLELQLEEQKQIRIQEAKIIEEKAAKIKEWVTVKLNELELENQNLRLINQNQTEEIKTIQSKLQEVQGKKSVVSTPKLSEGQRLSSLTFGCFLSRARSPPQVVKSEETSKISSKEPEFTEGRDIEEMEIPEKSVDNQIQENNRGQRTLHQGPCTSEQNRKTGTSFVMDGGTSQNSGAPGSDWSSDEEDGSKGRSKSRYTSTLSSHTSEDGVQSSRMGSETYLTASDDSSSIFEEETFGLKRPEHKKLYSWQQEAQWKAQSCPLGKGNSESRKKERDSSSDELNKKFQSQRLDYSSSSSEANTPSPILTPALSPKHPNPLLGKGTELGPSSTLPPPKLRIPNIFSLSIALAKRHLSQPQLSSDRMFGTNRNAISMIRPLRPQETDLDLVDGDNTEILETMDTNCDDGLFSYDCLESPCSEDQEACDLAKKAACGKPPTPPLHRFPSWESRIYAVAKSGIRMSEAFNMENVHKHSAAVLSYTTSGLYTSLIYKNMTTPVYTTLKGKATQISSSPFLDDSSGSEEEDSSRSSSRTSESDSRSRSGPGSPRALKRGVSLSSVASESDYAIPPDAYSADLEYSQPEQKLLKTCSSSSDNGKNEPLEKSGYLLKMSGKVKTWKRRWFVLKGGELLYYKSPSDVIRKPQGHIELSASCSILRGDNKQTVQLTTEKHTYYLTADSPNILEEWIKVLQNVLRVQAANPLFLQPEGKPTVKGLLTKVKHGYSKRVWCKLIGKTLYYFRSQEDKFPLGQIKLWEAKVEEVDRSCDSDEDYEASGRSLLSTHYTIVIHPKDQGPTYLLIGSKHEKDTWLYHLTVAAGSNNVNVGSEFEQLVCKLLNIEGEPSSQIWRHPTLCHSKEGIISPLTTLPSEALQTEAIKLFKTCQLFINAAVDSPAIDYHISLAQSALQICLTHPELQNEICCQLIKQTRWRQPQNQPGPLQGWQLLALCVGLFLPHHPFLWLLRLHLKRNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSLPFSIPVHFMNGIYQVVGFDASTTVEEFLNTLNQDTGMRKPAQSGFALFTDDPSGRDLEHCLQGNIKICDIISKWEQASKEQQPGKWEGTRTVRLTYKNRLYFSMQVRGETDREKLLLMYQTNDQIVNGLFPLNRDLALEMAALLAQVEIGDFERPFSTPAGHVTNQCKANQTLKQVIEKFYPKRYRDDSSEEQLRQLCQRLSTRWMALRGHSAADCVRIYLTVARKWPFFGAKLFLAKPITPSSLGSTFMWLAVHEDGLSILEYNSMRLIVSYVYKSLMTFGGYQDDFMVVINNVHSKEKPTEKLIFAMAKSKILEITLLIASYINNFYQQKAVHHLSAPALLSAQTQGFQARVMGSQPLLASSRPTKGPTLL

Target ORF information:

RefSeq Version	XM_012172698.3
Organism	Ovis aries(sheep)
Definition	Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012172698.3

ORF Insert Sequence:

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAGCTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GCCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTG TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCAC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTTGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GCCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCGGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA GATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC CAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGTGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAATCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHH2 ( XM_004005992.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004006041.2 Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X1, mRNA.

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CloneID	OSm77298
Clone ID Related Accession (Same CDS sequence)	XM_012172698.3 , XM_004005992.3
Accession Version	XM_004005992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	pleckstrin homology domain-containing family H member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_004005992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAGCTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GCCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTG TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCAC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTTGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GCCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCGGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA GATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC CAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGTGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAATCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004006041.2
CDS	103..4578
Translation	MAELSETEGPVDWKERCVALESQLMKFRVQASKIRELLAEKMQQLERQVIDAERQAEKAFQQVQVMEEKLKAANIQTSESETRLYKKCQDLETLIQEKEDIIQNLELQLEEQKQIRIQEAKIIEEKAAKIKEWVTVKLNELELENQNLRLINQNQTEEIKTIQSKLQEVQGKKSVVSTPKLSEGQRLSSLTFGCFLSRARSPPQVVKSEETSKISSKEPEFTEGRDIEEMEIPEKSVDNQIQENNRGQRTLHQGPCTSEQNRKTGTSFVMDGGTSQNSGAPGSDWSSDEEDGSKGRSKSRYTSTLSSHTSEDGVQSSRMGSETYLTASDDSSSIFEEETFGLKRPEHKKLYSWQQEAQWKAQSCPLGKGNSESRKKERDSSSDELNKKFQSQRLDYSSSSSEANTPSPILTPALSPKHPNPLLGKGTELGPSSTLPPPKLRIPNIFSLSIALAKRHLSQPQLSSDRMFGTNRNAISMIRPLRPQETDLDLVDGDNTEILETMDTNCDDGLFSYDCLESPCSEDQEACDLAKKAACGKPPTPPLHRFPSWESRIYAVAKSGIRMSEAFNMENVHKHSAAVLSYTTSGLYTSLIYKNMTTPVYTTLKGKATQISSSPFLDDSSGSEEEDSSRSSSRTSESDSRSRSGPGSPRALKRGVSLSSVASESDYAIPPDAYSADLEYSQPEQKLLKTCSSSSDNGKNEPLEKSGYLLKMSGKVKTWKRRWFVLKGGELLYYKSPSDVIRKPQGHIELSASCSILRGDNKQTVQLTTEKHTYYLTADSPNILEEWIKVLQNVLRVQAANPLFLQPEGKPTVKGLLTKVKHGYSKRVWCKLIGKTLYYFRSQEDKFPLGQIKLWEAKVEEVDRSCDSDEDYEASGRSLLSTHYTIVIHPKDQGPTYLLIGSKHEKDTWLYHLTVAAGSNNVNVGSEFEQLVCKLLNIEGEPSSQIWRHPTLCHSKEGIISPLTTLPSEALQTEAIKLFKTCQLFINAAVDSPAIDYHISLAQSALQICLTHPELQNEICCQLIKQTRWRQPQNQPGPLQGWQLLALCVGLFLPHHPFLWLLRLHLKRNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSLPFSIPVHFMNGIYQVVGFDASTTVEEFLNTLNQDTGMRKPAQSGFALFTDDPSGRDLEHCLQGNIKICDIISKWEQASKEQQPGKWEGTRTVRLTYKNRLYFSMQVRGETDREKLLLMYQTNDQIVNGLFPLNRDLALEMAALLAQVEIGDFERPFSTPAGHVTNQCKANQTLKQVIEKFYPKRYRDDSSEEQLRQLCQRLSTRWMALRGHSAADCVRIYLTVARKWPFFGAKLFLAKPITPSSLGSTFMWLAVHEDGLSILEYNSMRLIVSYVYKSLMTFGGYQDDFMVVINNVHSKEKPTEKLIFAMAKSKILEITLLIASYINNFYQQKAVHHLSAPALLSAQTQGFQARVMGSQPLLASSRPTKGPTLL

Target ORF information:

RefSeq Version	XM_004005992.3
Organism	Ovis aries(sheep)
Definition	Ovis aries pleckstrin homology, MyTH4 and FERM domain containing H2 (PLEKHH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004005992.3

ORF Insert Sequence:

ATGGCAGAGC TTTCTGAGAC AGAAGGGCCA GTAGATTGGA AAGAACGTTG TGTAGCCCTG 
GAGTCCCAGC TCATGAAATT TAGAGTTCAA GCAAGCAAGA TACGAGAGCT CTTAGCAGAG 
AAGATGCAAC AGCTTGAGAG ACAAGTTATT GATGCTGAGC GTCAAGCAGA AAAAGCTTTT 
CAACAGGTAC AAGTTATGGA AGAGAAGTTA AAAGCAGCTA ATATTCAAAC CAGTGAATCA 
GAGACAAGGT TGTATAAAAA GTGTCAAGAT CTGGAAACTC TAATACAGGA AAAAGAAGAC 
ATCATTCAAA ACTTGGAACT GCAACTTGAA GAGCAGAAAC AAATAAGGAT TCAAGAAGCT 
AAAATAATAG AAGAGAAAGC AGCTAAGATC AAAGAATGGG TAACAGTTAA GTTGAATGAG 
TTGGAATTGG AGAATCAGAA TCTTCGTTTG ATCAACCAAA ACCAAACTGA AGAGATAAAA 
ACAATACAGT CAAAGCTACA AGAAGTTCAA GGAAAGAAGT CAGTTGTTTC CACACCAAAG 
CTTTCAGAAG GCCAGCGTCT GAGCAGTCTG ACCTTTGGGT GCTTTTTGTC CCGAGCAAGG 
AGTCCTCCTC AAGTTGTAAA ATCTGAGGAA ACGAGCAAGA TATCATCTAA AGAACCTGAG 
TTCACAGAAG GAAGAGACAT AGAAGAGATG GAAATTCCAG AAAAATCTGT TGATAACCAA 
ATTCAAGAAA ACAACCGAGG TCAGAGAACA CTGCATCAAG GCCCTTGCAC GTCAGAGCAG 
AATCGTAAAA CAGGGACAAG CTTTGTGATG GATGGTGGCA CATCCCAGAA TTCTGGGGCT 
CCCGGGAGTG ACTGGAGTTC CGATGAGGAA GATGGGAGCA AAGGAAGATC CAAGTCCAGG 
TACACGTCTA CCCTTTCCAG CCACACATCA GAGGATGGGG TCCAAAGTAG CAGAATGGGC 
AGTGAGACAT ATCTCACAGC CTCTGACGAC AGCAGTTCGA TATTTGAAGA AGAGACCTTT 
GGCCTAAAGA GACCGGAACA CAAGAAGCTG TATTCTTGGC AACAGGAGGC ACAGTGGAAG 
GCTCAGAGTT GTCCTCTCGG AAAGGGAAAT TCCGAATCAA GGAAAAAGGA GCGTGATAGT 
TCCTCAGACG AACTCAATAA AAAATTTCAG TCTCAGAGGT TGGATTATTC GTCTTCCTCG 
AGTGAAGCTA ACACCCCAAG CCCTATTCTG ACCCCAGCTT TGAGTCCAAA GCATCCTAAC 
CCACTCCTTG GAAAAGGCAC AGAACTAGGA CCTTCCTCCA CTCTGCCACC CCCCAAATTA 
AGGATTCCTA ATATTTTCAG CTTAAGCATC GCACTAGCCA AAAGGCACCT AAGCCAGCCA 
CAGTTAAGTT CCGACAGGAT GTTTGGCACA AATAGAAACG CTATAAGCAT GATACGGCCA 
CTGAGACCTC AGGAAACAGA TCTTGATCTC GTTGATGGCG ACAATACAGA AATTTTAGAG 
ACTATGGACA CTAATTGTGA TGATGGATTA TTTTCCTACG ACTGCTTGGA ATCCCCATGT 
TCAGAGGACC AGGAAGCCTG CGACTTGGCA AAGAAAGCAG CCTGTGGCAA ACCTCCAACT 
CCACCTCTGC ACCGGTTTCC CTCTTGGGAA AGCAGAATTT ATGCTGTAGC CAAATCAGGC 
ATTCGAATGT CTGAGGCCTT CAATATGGAG AATGTTCATA AACATTCTGC TGCAGTCCTT 
TCCTATACCA CATCAGGACT TTATACATCT CTGATATACA AGAACATGAC AACCCCAGTG 
TATACAACTT TGAAGGGGAA GGCAACCCAG ATAAGCAGCA GCCCGTTCCT GGATGACTCC 
TCTGGGTCAG AAGAAGAAGA CAGTTCAAGA TCCAGCTCCC GGACTTCGGA GTCAGACTCT 
CGCAGTCGGA GTGGGCCAGG CAGCCCCAGA GCCTTGAAGC GAGGTGTGTC TCTCTCTTCT 
GTGGCTTCTG AAAGCGATTA TGCTATTCCC CCAGATGCTT ACTCCGCAGA CTTGGAGTAC 
TCACAGCCAG AGCAGAAGCT CCTGAAAACT TGCTCATCTT CCAGTGATAA TGGGAAAAAT 
GAACCACTGG AAAAATCTGG CTACTTATTA AAAATGAGTG GTAAAGTCAA GACTTGGAAG 
CGGAGATGGT TTGTTCTTAA AGGTGGTGAA TTACTTTACT ACAAATCTCC GAGCGATGTA 
ATCAGAAAAC CACAGGGCCA TATTGAACTT AGTGCATCCT GCAGCATTTT AAGAGGGGAT 
AACAAGCAAA CAGTTCAGTT GACCACTGAA AAACACACAT ACTATCTGAC TGCAGATTCC 
CCCAATATAT TGGAGGAATG GATTAAAGTG TTACAAAACG TTCTTCGAGT GCAAGCTGCC 
AACCCCCTTT TCCTGCAACC TGAGGGCAAA CCAACGGTGA AGGGACTGCT AACCAAGGTA 
AAACATGGTT ACTCCAAGAG AGTCTGGTGT AAACTTATAG GAAAAACATT ATATTATTTT 
CGTAGTCAAG AAGATAAGTT TCCTTTAGGT CAGATCAAAC TCTGGGAGGC TAAAGTTGAA 
GAGGTTGACA GATCCTGTGA TTCAGATGAA GATTATGAAG CCAGTGGGCG AAGTCTGTTA 
TCCACACATT ACACTATTGT GATCCACCCC AAAGACCAAG GTCCAACTTA CCTCCTAATT 
GGATCCAAGC ATGAAAAGGA CACTTGGCTT TATCACCTGA CTGTTGCAGC TGGAAGTAAC 
AATGTGAATG TTGGATCTGA ATTTGAACAG CTTGTTTGCA AGTTGCTAAA TATTGAAGGG 
GAACCTTCCT CTCAGATATG GAGACACCCT ACTTTGTGTC ACAGCAAAGA AGGAATCATT 
TCCCCTCTGA CAACACTACC TTCAGAAGCT CTACAGACAG AAGCAATTAA ATTATTTAAG 
ACCTGCCAAC TTTTTATAAA TGCGGCAGTC GACTCTCCCG CAATTGATTA CCACATATCT 
CTAGCCCAGA GTGCTTTGCA GATCTGTCTG ACACATCCTG AGCTACAGAA TGAAATTTGC 
TGTCAGCTTA TTAAACAGAC AAGATGGAGA CAGCCGCAGA ATCAACCAGG ACCACTGCAG 
GGCTGGCAAC TCTTGGCTCT CTGTGTTGGG CTCTTCCTTC CCCATCATCC TTTCCTGTGG 
CTCCTCAGGC TCCACCTAAA GAGAAATGCA GATTCCAGGA CAGAATTTGG AAAATATGCC 
ATTTACTGCC AGAGATGTGT AGAAAGAACA CAACAGAATG GAGATCGAGA AGCAAGACCC 
TCAAGAATGG AGATTCTTTC CACCCTTCTT CGAAACCCAT ATCATCATTC TTTGCCCTTC 
AGTATCCCTG TGCATTTCAT GAATGGAATA TATCAGGTAG TTGGATTTGA TGCATCCACC 
ACAGTGGAAG AGTTTTTGAA TACTTTGAAC CAAGATACAG GGATGAGGAA GCCAGCACAG 
TCTGGATTTG CATTGTTCAC AGATGATCCT TCCGGCCGGG ATTTAGAGCA TTGTCTTCAA 
GGAAACATCA AGATTTGTGA CATTATCTCT AAATGGGAAC AGGCTTCTAA AGAACAGCAG 
CCTGGGAAAT GGGAAGGTAC GAGAACGGTG CGTCTCACTT ATAAAAACAG ATTATATTTC 
TCAATGCAAG TTCGAGGAGA GACTGATAGA GAAAAGTTGC TGTTAATGTA TCAGACAAAT 
GATCAAATAG TAAATGGACT TTTTCCTCTG AACAGGGACC TGGCATTAGA AATGGCAGCT 
CTTTTAGCTC AGGTGGAGAT TGGAGATTTT GAAAGACCTT TTTCCACTCC AGCAGGTCAT 
GTTACTAATC AGTGCAAAGC CAATCAAACT CTAAAACAAG TCATAGAGAA ATTTTATCCT 
AAAAGGTATA GAGATGACTC TTCTGAAGAA CAGCTAAGGC AGCTTTGCCA GCGACTGTCA 
ACCAGGTGGA TGGCCCTCCG AGGACACAGT GCTGCTGATT GTGTGCGAAT CTATTTGACA 
GTAGCCAGGA AGTGGCCATT CTTTGGTGCC AAGCTATTTC TTGCAAAACC CATAACCCCA 
TCGTCCCTTG GAAGTACGTT CATGTGGCTG GCTGTACATG AGGATGGTTT AAGCATCTTA 
GAATACAACT CCATGAGGTT AATAGTCAGC TATGTATACA AGAGTCTAAT GACCTTTGGA 
GGTTATCAAG ATGATTTTAT GGTAGTCATT AACAATGTGC ATTCAAAGGA GAAACCAACA 
GAGAAATTGA TTTTTGCTAT GGCAAAATCC AAGATCCTTG AAATCACCCT TTTGATCGCC 
AGCTACATAA ACAACTTCTA CCAGCAAAAG GCAGTTCACC ACCTCTCTGC TCCAGCGCTG 
CTCTCAGCTC AGACCCAGGG TTTCCAAGCC AGGGTGATGG GAAGCCAGCC CCTTCTGGCA 
AGCAGCAGAC CAACCAAAGG CCCCACTTTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PLEKHH2 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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