ATG2B gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_024981791.1	XP_024837559.1	autophagy-related protein 2 homolog B isoform X5
XM_005222199.2	XP_005222256.1	autophagy-related protein 2 homolog B isoform X1
XM_005222200.3	XP_005222257.1	autophagy-related protein 2 homolog B isoform X2
XM_005222200.4	XP_005222257.1	autophagy-related protein 2 homolog B isoform X3
XM_024981789.1	XP_024837557.1	autophagy-related protein 2 homolog B isoform X1
NM_001206753.1	NP_001193682.1	autophagy-related protein 2 homolog B
XM_005222199.3	XP_005222256.1	autophagy-related protein 2 homolog B isoform X2
XM_024981790.1	XP_024837558.1	autophagy-related protein 2 homolog B isoform X4
XM_024981792.1	XP_024837560.1	autophagy-related protein 2 homolog B isoform X6
XM_010817249.2	XP_010815551.1	autophagy-related protein 2 homolog B isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36840	NM_001206753.1 Latest version!	Bos taurus autophagy related 2B (ATG2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa228330	XM_024981789.1 Latest version!	Bos taurus autophagy related 2B (ATG2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa228331	XM_024981790.1 Latest version!	Bos taurus autophagy related 2B (ATG2B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa228332	XM_024981791.1 Latest version!	Bos taurus autophagy related 2B (ATG2B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa228333	XM_024981792.1 Latest version!	Bos taurus autophagy related 2B (ATG2B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa31115	XM_005222199.3 Latest version!	Bos taurus autophagy related 2B (ATG2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa31116	XM_005222200.4 Latest version!	Bos taurus autophagy related 2B (ATG2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa50164	XM_010817249.2 Latest version!	Bos taurus autophagy related 2B (ATG2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OBa31116	XM_005222200.3	Bos taurus autophagy related 2B (ATG2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa31115	XM_005222199.2	Bos taurus autophagy related 2B (ATG2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

ATG2B ( NM_001206753.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193682.1 Bos taurus autophagy related 2B (ATG2B), mRNA.

Price & Availability↑

CloneID	OBa36840
Clone ID Related Accession (Same CDS sequence)	NM_001206753.1
Accession Version	NM_001206753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527436800000
Organism	Bos taurus(cattle)
Product	autophagy-related protein 2 homolog B
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000021.1. On or before May 6, 2011 this sequence version replaced XM_001787556.1, XM_002696832.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTCAATG ATTTGCTGCT CTGGGAACCA ACAGCTCCTT CGCCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCGTTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAACG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAGCAGC GGCTCGTTAC TTTGTGTGAC GAAGTATGAA 
GGCTTCGACG ACAAGCACTA CGTGTGCCTT CACTGCGGCA GTTTCAGCCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATATAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCACT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCTCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTCGCCTTGG ATAAATCATC TTCTACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCGC ACTTTGAATT GCACTGTTCG 
AGTGACGTCG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATATATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTGAA GGTAGATTCT GTTGGTCGGT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA GATATTACGA GATTTGATGA GTGATGCTAT GGAGGAGATT 
GACATGCAAG AAGCAGCTTC ATCTGTAAAA CCACAGTCTA ATGGTGTTTC TGATGAAAAA 
TCTCAAGTTC AGGAACCATG TTGTTCAGAC CTCTTCCTGT TTCCTGATGA GAGTGGAAAC 
GTGTCCCAGG AGCCTGGCCC CACTTACGCC TCATTCACCC ACCACTTGAT TAGTGATGCA 
GTGACAGGCG TGCCCACGGA AAATGACGAC TTTTGCATTC TTTTTGCACC AAGAGCAACT 
GTTCAGGAGA AGGAAGAAGA ACCAGTTGTA AAGATAATGG TTGATGATCC AATTGTGATA 
AAAGAGAATT ATTTTAGTCT GCCTGTTAAG AAGACAGATA CGAGCAAAGC CCCGTTACAC 
TTTCCCATTC CTGTCGTTCG GTACCTGGTT AAAGAAGTCT CTCTTGTTTG GCATCTTTAT 
GGAGGAAAAG ATTTTGGAAC AGCCCCTCCT ACTTCTCCAG CTAAAAGTTA CATTAGTCCC 
CATAGCTCAC CTTCTCACAC ACCTACAAGA CATGGACATA ATATGGTTTG TGGGGGAAAA 
GGAAGAAACC ATGACTTTTT AATGGAAATA CAGTTAAGCA AGGTGAAGTT TCAGCACGAG 
GTGTACCCGC CCTGCAAACC AGAATGCGAG TCGAGCCTTG TGGAGCACCC GGTGTCCCGG 
CAGGTGTTCA TCGTGCAGGA TCTTGAAATT CGAGATCGTC TGGCAACGTC ACAAATGAAT 
AAATTTTTGT ACCTGTATTG CAGTAAAGAA ATGCCTCGGA AAGCTCATTC CAACATGTTG 
ACAATTAAAG CACTACACGT GCGTCCAGAG TCTGGCAGGT CCCCACAGGA GTGCTGCCTC 
AGAGTATCAC TAATGCCACT TCGCCTCAAT ATTGACCAGG ATGCCTTGTT CTTCTTGAAG 
GATTTCTTCA CAAGTCTTTC TACAGAAGTA GAGCTGCTCA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CAGGGGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTAAG CGCCTCAAAA 
GAGCCAAATC TAGTTCTTTC TTTCCCTGGG CCAAAGCAGC CTTCCCCAAA TGGCAGTGCC 
AATTCAGTGC AAGCGGTTAA TGGGGAAGAA GAGAAGGACT TCTCTCCTGA AGAAGCGTTG 
TTTAGTGATC AGCCTGTGTT TTTTAGAGAA TTTAGATTCA CATCAGAAGT TCCTATTCGA 
CTTGATTATC ATGGGAAACA TGTATCAATG GATCAGGGTA CATTAGCTGG GATTTTGATT 
GGTCTCGCCC AGTTAAACTG CTCTGAACTA AAGCTGAAGA GGCTTTTCTA TCGGCATGGT 
TTGCTAGGTG TGGACAAATT ATTCTCATAC GCAATCACCG AGTGGCTTAC TGACATTAAG 
AAGAACCAGC TGCCAGGAAT CCTGGGAGGT GTTGGGCCTA TGCATTCACT AGTACAGCTA 
GTGCAAGGCC TCAAGGACTT GGTCTGGTTA CCAATTGAGC AGTACCGAAA GGACGGCCGC 
ATTGTCAGAG GGTTTCAGAG AGGGGCTGCC TCCTTTGGCA CCTCGACAGC AATGGCTGCT 
CTCGAGCTCA CGAACAGAAT GGTTCAAACC ATACAGGCAG CAGCGGAGAC TGCGTATGAC 
ATGGTGTCTC CAGGCACCCT TTCTATCGAG CCCAAGAAGG TCAGGAGGTT CCCCCATCAC 
CGGTTAGCCC ACCAGCCAGT GGACCTGAGG GAAGGGGTGG CCAAGGCCTA CAGTGTCGTG 
AAGGAGGGAA TCGCAGACAC GGCTCAGACC ATTTACGAAA CTGCTGCTCG GGAACACGAG 
AGCCGGGGGG TGACGGGCGC CGTGGGGGAA GTCCTGCGCC AGCTCCCCCC GGCCGTGGTG 
AAACCTCTGA TCGTCGCCAC AGAAGCGACA TCGAATGTCT TGGGTGGCAT GAGAAACCAG 
ATCAGACCAG ATGTTCGGCA GGACGAGTCG CAGAAGTGGC GCCATGGGGA CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193682.1
CDS	1..6237
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASASEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTVPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSISVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQAGQARLSSIPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWLEFSSGSLLCVTKYEGFDDKHYVCLHCGSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDPIVIKENYFSLPVKKTDTSKAPLHFPIPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMTPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	NM_001206753.1
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy related 2B (ATG2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206753.1

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTCAATG ATTTGCTGCT CTGGGAACCA ACAGCTCCTT CGCCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCGTTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAACG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAGCAGC GGCTCGTTAC TTTGTGTGAC GAAGTATGAA 
GGCTTCGACG ACAAGCACTA CGTGTGCCTT CACTGCGGCA GTTTCAGCCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATATAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCACT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCTCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTCGCCTTGG ATAAATCATC TTCTACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCGC ACTTTGAATT GCACTGTTCG 
AGTGACGTCG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATATATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTGAA GGTAGATTCT GTTGGTCGGT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA GATATTACGA GATTTGATGA GTGATGCTAT GGAGGAGATT 
GACATGCAAG AAGCAGCTTC ATCTGTAAAA CCACAGTCTA ATGGTGTTTC TGATGAAAAA 
TCTCAAGTTC AGGAACCATG TTGTTCAGAC CTCTTCCTGT TTCCTGATGA GAGTGGAAAC 
GTGTCCCAGG AGCCTGGCCC CACTTACGCC TCATTCACCC ACCACTTGAT TAGTGATGCA 
GTGACAGGCG TGCCCACGGA AAATGACGAC TTTTGCATTC TTTTTGCACC AAGAGCAACT 
GTTCAGGAGA AGGAAGAAGA ACCAGTTGTA AAGATAATGG TTGATGATCC AATTGTGATA 
AAAGAGAATT ATTTTAGTCT GCCTGTTAAG AAGACAGATA CGAGCAAAGC CCCGTTACAC 
TTTCCCATTC CTGTCGTTCG GTACCTGGTT AAAGAAGTCT CTCTTGTTTG GCATCTTTAT 
GGAGGAAAAG ATTTTGGAAC AGCCCCTCCT ACTTCTCCAG CTAAAAGTTA CATTAGTCCC 
CATAGCTCAC CTTCTCACAC ACCTACAAGA CATGGACATA ATATGGTTTG TGGGGGAAAA 
GGAAGAAACC ATGACTTTTT AATGGAAATA CAGTTAAGCA AGGTGAAGTT TCAGCACGAG 
GTGTACCCGC CCTGCAAACC AGAATGCGAG TCGAGCCTTG TGGAGCACCC GGTGTCCCGG 
CAGGTGTTCA TCGTGCAGGA TCTTGAAATT CGAGATCGTC TGGCAACGTC ACAAATGAAT 
AAATTTTTGT ACCTGTATTG CAGTAAAGAA ATGCCTCGGA AAGCTCATTC CAACATGTTG 
ACAATTAAAG CACTACACGT GCGTCCAGAG TCTGGCAGGT CCCCACAGGA GTGCTGCCTC 
AGAGTATCAC TAATGCCACT TCGCCTCAAT ATTGACCAGG ATGCCTTGTT CTTCTTGAAG 
GATTTCTTCA CAAGTCTTTC TACAGAAGTA GAGCTGCTCA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CAGGGGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTAAG CGCCTCAAAA 
GAGCCAAATC TAGTTCTTTC TTTCCCTGGG CCAAAGCAGC CTTCCCCAAA TGGCAGTGCC 
AATTCAGTGC AAGCGGTTAA TGGGGAAGAA GAGAAGGACT TCTCTCCTGA AGAAGCGTTG 
TTTAGTGATC AGCCTGTGTT TTTTAGAGAA TTTAGATTCA CATCAGAAGT TCCTATTCGA 
CTTGATTATC ATGGGAAACA TGTATCAATG GATCAGGGTA CATTAGCTGG GATTTTGATT 
GGTCTCGCCC AGTTAAACTG CTCTGAACTA AAGCTGAAGA GGCTTTTCTA TCGGCATGGT 
TTGCTAGGTG TGGACAAATT ATTCTCATAC GCAATCACCG AGTGGCTTAC TGACATTAAG 
AAGAACCAGC TGCCAGGAAT CCTGGGAGGT GTTGGGCCTA TGCATTCACT AGTACAGCTA 
GTGCAAGGCC TCAAGGACTT GGTCTGGTTA CCAATTGAGC AGTACCGAAA GGACGGCCGC 
ATTGTCAGAG GGTTTCAGAG AGGGGCTGCC TCCTTTGGCA CCTCGACAGC AATGGCTGCT 
CTCGAGCTCA CGAACAGAAT GGTTCAAACC ATACAGGCAG CAGCGGAGAC TGCGTATGAC 
ATGGTGTCTC CAGGCACCCT TTCTATCGAG CCCAAGAAGG TCAGGAGGTT CCCCCATCAC 
CGGTTAGCCC ACCAGCCAGT GGACCTGAGG GAAGGGGTGG CCAAGGCCTA CAGTGTCGTG 
AAGGAGGGAA TCGCAGACAC GGCTCAGACC ATTTACGAAA CTGCTGCTCG GGAACACGAG 
AGCCGGGGGG TGACGGGCGC CGTGGGGGAA GTCCTGCGCC AGCTCCCCCC GGCCGTGGTG 
AAACCTCTGA TCGTCGCCAC AGAAGCGACA TCGAATGTCT TGGGTGGCAT GAGAAACCAG 
ATCAGACCAG ATGTTCGGCA GGACGAGTCG CAGAAGTGGC GCCATGGGGA CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_024981789.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837557.1 Bos taurus autophagy related 2B (ATG2B), transcript variant X1, mRNA.

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CloneID	OBa228330
Clone ID Related Accession (Same CDS sequence)	XM_024981789.1
Accession Version	XM_024981789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
GCCTCAGAAG CTCAGGAGTT GGCCCAGGAA GTTCCTAGAC CAGTGGCTTC TCGCAGGAAG 
GGGCCAGGGT TTGCGTCATC GCTTGTCACC TTGCTGGCAT TCACACTAGG CTTTACGATG 
AGCCTGTGCT TTTGGCAGTC CTTCATGATC TTCAATGATT TGCTGCTCTG GGAACCAACA 
GCTCCTTCGC CAGTAGAGAC ATTTGAAAAT ATTTCGTATG GTATTGGGCT TTCAGTAGCC 
AGTCAGCTGA TTAATACCTT CAGCAAGGAT AGTTTTAGTC CGTTTAAATC TACAGTTCAC 
TATGATGAGG AAAGTGGATC TGAGGAGGAG ACTTTGCAGT ATTTTTCTAC TGTTGATCCC 
AATTATCGTT CTCGTAGGAA AAAAAAACTA GACTCCCAGA ACAAGAACTC ACAGAGTTTT 
CTCTCAGTTC TTCTGAGTAT TAATCATGGG TTAATTGCAG TGTTTACAGA TGTAAAGCAA 
GATAACGGAG ATCTGTTGGA AAACAAGCAT GGTGAATTCT GGTTAGAGTT CAGCAGCGGC 
TCGTTACTTT GTGTGACGAA GTATGAAGGC TTCGACGACA AGCACTACGT GTGCCTTCAC 
TGCGGCAGTT TCAGCCTCTA CCACAAAGGC ATAGTGGATG GAGCAGTTCC TTCTACAGAA 
ACACGACTGC CTAGTTCAAC CCGCCCACAT TGGTTAGAAC CTACTATTTA TTCCTCTGAA 
GAAGATGGCC TCAGTCGAGC TTCTTCAGAT GGTGTTGGAG GAGACACTTT GAATATGCTG 
TCTGTTGCAG TTAAAATAGT GTCTGATAAA TCAGAGTCCA ATACAAAGGA GTTTCTCATT 
GCTGTAGGGC TAAAAGGAGC TACTCTCCAG CATAGAATGC TTCCTTCTGG GCTTAGCTGG 
CATGAGCAGA TTTTATACTT CTTGAATATT GCTGATGAAC CTGTTCTGGG ATATAATCCT 
CCAACTTCAT TTACAACCTT TCATGTTCAT CTCTGGAGCT GTGCACTTGA TTATAGGCCT 
CTTTATTTGC CAATCCGATC TCTTCTTACT GTTGAAACAT TCAGCGTTTC AAGTAGTGTC 
GCCTTGGATA AATCATCTTC TACTCTCAGA ATAATTTTGG ATGAAGCTGC TTTACACCTG 
TCTGACAAGT GTAATACTGT TACTATAAAT TTGAATAGAG ATTATGTTCG TGTGATGGAT 
ATGGGACTTT TGGAATTAAC CATAACTGCA GTAAAGTCTG ATTCCGATGG AGAGCAAACT 
GAACCGCACT TTGAATTGCA CTGTTCGAGT GACGTCGTCC ATGTTAGAAC GTGCTCAGAC 
TCTTGTGCTG CTCTAATGAA TCTAATTCAA TATATTGCAA GCTATGGGGA CTTACATGCC 
ACTAACAAGG CAGACGTGAA GCCAGGAGCT CCTCAAAGAA AGGTGAAGAA TTCTCAGCCC 
CCTCAGGTAG ATTCTGTTGG TCGGTCATCC TCACGTGGTC CAGTACTTCC TGAAGCAGAT 
CAACAGATAT TACGAGATTT GATGAGTGAT GCTATGGAGG AGATTGACAT GCAAGAAGCA 
GCTTCATCTG TAAAACCACA GTCTAATGGT GTTTCTGATG AAAAATCTCA AGTTCAGGAA 
CCATGTTGTT CAGACCTCTT CCTGTTTCCT GATGAGAGTG GAAACGTGTC CCAGGAGCCT 
GGCCCCACTT ACGCCTCATT CACCCACCAC TTGATTAGTG ATGCAGTGAC AGGCGTGCCC 
ACGGAAAATG ACGACTTTTG CATTCTTTTT GCACCAAGAG CAACTGTTCA GGAGAAGGAA 
GAAGAACCAG TTGTAAAGAT AATGGTTGAT GATCCAATTG TGATAAAAGA GAATTATTTT 
AGTCTGCCTG TTAAGAAGAC AGATACGAGC AAAGCCCCGT TACACTTTCC CATTCCTGTC 
GTTCGGTACC TGGTTAAAGA AGTCTCTCTT GTTTGGCATC TTTATGGAGG AAAAGATTTT 
GGAACAGCCC CTCCTACTTC TCCAGCTAAA AGTTACATTA GTCCCCATAG CTCACCTTCT 
CACACACCTA CAAGACATGG ACATAATATG GTTTGTGGGG GAAAAGGAAG AAACCATGAC 
TTTTTAATGG AAATACAGTT AAGCAAGGTG AAGTTTCAGC ACGAGGTGTA CCCGCCCTGC 
AAACCAGAAT GCGAGTCGAG CCTTGTGGAG CACCCGGTGT CCCGGCAGGT GTTCATCGTG 
CAGGATCTTG AAATTCGAGA TCGTCTGGCA ACGTCACAAA TGAATAAATT TTTGTACCTG 
TATTGCAGTA AAGAAATGCC TCGGAAAGCT CATTCCAACA TGTTGACAAT TAAAGCACTA 
CACGTGCGTC CAGAGTCTGG CAGGTCCCCA CAGGAGTGCT GCCTCAGAGT ATCACTAATG 
CCACTTCGCC TCAATATTGA CCAGGATGCC TTGTTCTTCT TGAAGGATTT CTTCACAAGT 
CTTTCTACAG AAGTAGAGCT GCTCATGACT CCAGATCCAG AAGTTAAAAA GTCTCCAGGG 
GCTGATGTCA CCTGCAGTTT GCCAAGGCAT TTAAGCGCCT CAAAAGAGCC AAATCTAGTT 
CTTTCTTTCC CTGGGCCAAA GCAGCCTTCC CCAAATGGCA GTGCCAATTC AGTGCAAGCG 
GTTAATGGGG AAGAAGAGAA GGACTTCTCT CCTGAAGAAG CGTTGTTTAG TGATCAGCCT 
GTGTTTTTTA GAGAATTTAG ATTCACATCA GAAGTTCCTA TTCGACTTGA TTATCATGGG 
AAACATGTAT CAATGGATCA GGGTACATTA GCTGGGATTT TGATTGGTCT CGCCCAGTTA 
AACTGCTCTG AACTAAAGCT GAAGAGGCTT TTCTATCGGC ATGGTTTGCT AGGTGTGGAC 
AAATTATTCT CATACGCAAT CACCGAGTGG CTTACTGACA TTAAGAAGAA CCAGCTGCCA 
GGAATCCTGG GAGGTGTTGG GCCTATGCAT TCACTAGTAC AGCTAGTGCA AGGCCTCAAG 
GACTTGGTCT GGTTACCAAT TGAGCAGTAC CGAAAGGACG GCCGCATTGT CAGAGGGTTT 
CAGAGAGGGG CTGCCTCCTT TGGCACCTCG ACAGCAATGG CTGCTCTCGA GCTCACGAAC 
AGAATGGTTC AAACCATACA GGCAGCAGCG GAGACTGCGT ATGACATGGT GTCTCCAGGC 
ACCCTTTCTA TCGAGCCCAA GAAGGTCAGG AGGTTCCCCC ATCACCGGTT AGCCCACCAG 
CCAGTGGACC TGAGGGAAGG GGTGGCCAAG GCCTACAGTG TCGTGAAGGA GGGAATCGCA 
GACACGGCTC AGACCATTTA CGAAACTGCT GCTCGGGAAC ACGAGAGCCG GGGGGTGACG 
GGCGCCGTGG GGGAAGTCCT GCGCCAGCTC CCCCCGGCCG TGGTGAAACC TCTGATCGTC 
GCCACAGAAG CGACATCGAA TGTCTTGGGT GGCATGAGAA ACCAGATCAG ACCAGATGTT 
CGGCAGGACG AGTCGCAGAA GTGGCGCCAT GGGGACGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837557.1
CDS	16..6417
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASASEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTVPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSISVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQAGQARLSSIPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRASEAQELAQEVPRPVASRRKGPGFASSLVTLLAFTLGFTMSLCFWQSFMIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWLEFSSGSLLCVTKYEGFDDKHYVCLHCGSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKNSQPPQVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDPIVIKENYFSLPVKKTDTSKAPLHFPIPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMTPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_024981789.1
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy related 2B (ATG2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981789.1

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
GCCTCAGAAG CTCAGGAGTT GGCCCAGGAA GTTCCTAGAC CAGTGGCTTC TCGCAGGAAG 
GGGCCAGGGT TTGCGTCATC GCTTGTCACC TTGCTGGCAT TCACACTAGG CTTTACGATG 
AGCCTGTGCT TTTGGCAGTC CTTCATGATC TTCAATGATT TGCTGCTCTG GGAACCAACA 
GCTCCTTCGC CAGTAGAGAC ATTTGAAAAT ATTTCGTATG GTATTGGGCT TTCAGTAGCC 
AGTCAGCTGA TTAATACCTT CAGCAAGGAT AGTTTTAGTC CGTTTAAATC TACAGTTCAC 
TATGATGAGG AAAGTGGATC TGAGGAGGAG ACTTTGCAGT ATTTTTCTAC TGTTGATCCC 
AATTATCGTT CTCGTAGGAA AAAAAAACTA GACTCCCAGA ACAAGAACTC ACAGAGTTTT 
CTCTCAGTTC TTCTGAGTAT TAATCATGGG TTAATTGCAG TGTTTACAGA TGTAAAGCAA 
GATAACGGAG ATCTGTTGGA AAACAAGCAT GGTGAATTCT GGTTAGAGTT CAGCAGCGGC 
TCGTTACTTT GTGTGACGAA GTATGAAGGC TTCGACGACA AGCACTACGT GTGCCTTCAC 
TGCGGCAGTT TCAGCCTCTA CCACAAAGGC ATAGTGGATG GAGCAGTTCC TTCTACAGAA 
ACACGACTGC CTAGTTCAAC CCGCCCACAT TGGTTAGAAC CTACTATTTA TTCCTCTGAA 
GAAGATGGCC TCAGTCGAGC TTCTTCAGAT GGTGTTGGAG GAGACACTTT GAATATGCTG 
TCTGTTGCAG TTAAAATAGT GTCTGATAAA TCAGAGTCCA ATACAAAGGA GTTTCTCATT 
GCTGTAGGGC TAAAAGGAGC TACTCTCCAG CATAGAATGC TTCCTTCTGG GCTTAGCTGG 
CATGAGCAGA TTTTATACTT CTTGAATATT GCTGATGAAC CTGTTCTGGG ATATAATCCT 
CCAACTTCAT TTACAACCTT TCATGTTCAT CTCTGGAGCT GTGCACTTGA TTATAGGCCT 
CTTTATTTGC CAATCCGATC TCTTCTTACT GTTGAAACAT TCAGCGTTTC AAGTAGTGTC 
GCCTTGGATA AATCATCTTC TACTCTCAGA ATAATTTTGG ATGAAGCTGC TTTACACCTG 
TCTGACAAGT GTAATACTGT TACTATAAAT TTGAATAGAG ATTATGTTCG TGTGATGGAT 
ATGGGACTTT TGGAATTAAC CATAACTGCA GTAAAGTCTG ATTCCGATGG AGAGCAAACT 
GAACCGCACT TTGAATTGCA CTGTTCGAGT GACGTCGTCC ATGTTAGAAC GTGCTCAGAC 
TCTTGTGCTG CTCTAATGAA TCTAATTCAA TATATTGCAA GCTATGGGGA CTTACATGCC 
ACTAACAAGG CAGACGTGAA GCCAGGAGCT CCTCAAAGAA AGGTGAAGAA TTCTCAGCCC 
CCTCAGGTAG ATTCTGTTGG TCGGTCATCC TCACGTGGTC CAGTACTTCC TGAAGCAGAT 
CAACAGATAT TACGAGATTT GATGAGTGAT GCTATGGAGG AGATTGACAT GCAAGAAGCA 
GCTTCATCTG TAAAACCACA GTCTAATGGT GTTTCTGATG AAAAATCTCA AGTTCAGGAA 
CCATGTTGTT CAGACCTCTT CCTGTTTCCT GATGAGAGTG GAAACGTGTC CCAGGAGCCT 
GGCCCCACTT ACGCCTCATT CACCCACCAC TTGATTAGTG ATGCAGTGAC AGGCGTGCCC 
ACGGAAAATG ACGACTTTTG CATTCTTTTT GCACCAAGAG CAACTGTTCA GGAGAAGGAA 
GAAGAACCAG TTGTAAAGAT AATGGTTGAT GATCCAATTG TGATAAAAGA GAATTATTTT 
AGTCTGCCTG TTAAGAAGAC AGATACGAGC AAAGCCCCGT TACACTTTCC CATTCCTGTC 
GTTCGGTACC TGGTTAAAGA AGTCTCTCTT GTTTGGCATC TTTATGGAGG AAAAGATTTT 
GGAACAGCCC CTCCTACTTC TCCAGCTAAA AGTTACATTA GTCCCCATAG CTCACCTTCT 
CACACACCTA CAAGACATGG ACATAATATG GTTTGTGGGG GAAAAGGAAG AAACCATGAC 
TTTTTAATGG AAATACAGTT AAGCAAGGTG AAGTTTCAGC ACGAGGTGTA CCCGCCCTGC 
AAACCAGAAT GCGAGTCGAG CCTTGTGGAG CACCCGGTGT CCCGGCAGGT GTTCATCGTG 
CAGGATCTTG AAATTCGAGA TCGTCTGGCA ACGTCACAAA TGAATAAATT TTTGTACCTG 
TATTGCAGTA AAGAAATGCC TCGGAAAGCT CATTCCAACA TGTTGACAAT TAAAGCACTA 
CACGTGCGTC CAGAGTCTGG CAGGTCCCCA CAGGAGTGCT GCCTCAGAGT ATCACTAATG 
CCACTTCGCC TCAATATTGA CCAGGATGCC TTGTTCTTCT TGAAGGATTT CTTCACAAGT 
CTTTCTACAG AAGTAGAGCT GCTCATGACT CCAGATCCAG AAGTTAAAAA GTCTCCAGGG 
GCTGATGTCA CCTGCAGTTT GCCAAGGCAT TTAAGCGCCT CAAAAGAGCC AAATCTAGTT 
CTTTCTTTCC CTGGGCCAAA GCAGCCTTCC CCAAATGGCA GTGCCAATTC AGTGCAAGCG 
GTTAATGGGG AAGAAGAGAA GGACTTCTCT CCTGAAGAAG CGTTGTTTAG TGATCAGCCT 
GTGTTTTTTA GAGAATTTAG ATTCACATCA GAAGTTCCTA TTCGACTTGA TTATCATGGG 
AAACATGTAT CAATGGATCA GGGTACATTA GCTGGGATTT TGATTGGTCT CGCCCAGTTA 
AACTGCTCTG AACTAAAGCT GAAGAGGCTT TTCTATCGGC ATGGTTTGCT AGGTGTGGAC 
AAATTATTCT CATACGCAAT CACCGAGTGG CTTACTGACA TTAAGAAGAA CCAGCTGCCA 
GGAATCCTGG GAGGTGTTGG GCCTATGCAT TCACTAGTAC AGCTAGTGCA AGGCCTCAAG 
GACTTGGTCT GGTTACCAAT TGAGCAGTAC CGAAAGGACG GCCGCATTGT CAGAGGGTTT 
CAGAGAGGGG CTGCCTCCTT TGGCACCTCG ACAGCAATGG CTGCTCTCGA GCTCACGAAC 
AGAATGGTTC AAACCATACA GGCAGCAGCG GAGACTGCGT ATGACATGGT GTCTCCAGGC 
ACCCTTTCTA TCGAGCCCAA GAAGGTCAGG AGGTTCCCCC ATCACCGGTT AGCCCACCAG 
CCAGTGGACC TGAGGGAAGG GGTGGCCAAG GCCTACAGTG TCGTGAAGGA GGGAATCGCA 
GACACGGCTC AGACCATTTA CGAAACTGCT GCTCGGGAAC ACGAGAGCCG GGGGGTGACG 
GGCGCCGTGG GGGAAGTCCT GCGCCAGCTC CCCCCGGCCG TGGTGAAACC TCTGATCGTC 
GCCACAGAAG CGACATCGAA TGTCTTGGGT GGCATGAGAA ACCAGATCAG ACCAGATGTT 
CGGCAGGACG AGTCGCAGAA GTGGCGCCAT GGGGACGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_024981790.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837558.1 Bos taurus autophagy related 2B (ATG2B), transcript variant X4, mRNA.

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CloneID	OBa228331
Clone ID Related Accession (Same CDS sequence)	XM_024981790.1
Accession Version	XM_024981790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	autophagy-related protein 2 homolog B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTCAATG ATTTGCTGCT CTGGGAACCA ACAGCTCCTT CGCCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCGTTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAACG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAGCAGC GGCTCGTTAC TTTGTGTGAC GAAGTATGAA 
GGCTTCGACG ACAAGCACTA CGTGTGCCTT CACTGCGGCA GTTTCAGCCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATATAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCACT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCTCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTCGCCTTGG ATAAATCATC TTCTACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCGC ACTTTGAATT GCACTGTTCG 
AGTGACGTCG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATATATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTGAA GAATTCTCAG CCCCCTCAGG TAGATTCTGT TGGTCGGTCA 
TCCTCACGTG GTCCAGTACT TCCTGAAGCA GATCAACAGA TATTACGAGA TTTGATGAGT 
GATGCTATGG AGGAGATTGA CATGCAAGAA GCAGCTTCAT CTGTAAAACC ACAGTCTAAT 
GGTGTTTCTG ATGAAAAATC TCAAGTTCAG GAACCATGTT GTTCAGACCT CTTCCTGTTT 
CCTGATGAGA GTGGAAACGT GTCCCAGGAG CCTGGCCCCA CTTACGCCTC ATTCACCCAC 
CACTTGATTA GTGATGCAGT GACAGGCGTG CCCACGGAAA ATGACGACTT TTGCATTCTT 
TTTGCACCAA GAGCAACTGT TCAGGAGAAG GAAGAAGAAC CAGTTGTAAA GATAATGGTT 
GATGATCCAA TTGTGATAAA AGAGAATTAT TTTAGTCTGC CTGTTAAGAA GACAGATACG 
AGCAAAGCCC CGTTACACTT TCCCATTCCT GTCGTTCGGT ACCTGGTTAA AGAAGTCTCT 
CTTGTTTGGC ATCTTTATGG AGGAAAAGAT TTTGGAACAG CCCCTCCTAC TTCTCCAGCT 
AAAAGTTACA TTAGTCCCCA TAGCTCACCT TCTCACACAC CTACAAGACA TGGACATAAT 
ATGGTTTGTG GGGGAAAAGG AAGAAACCAT GACTTTTTAA TGGAAATACA GTTAAGCAAG 
GTGAAGTTTC AGCACGAGGT GTACCCGCCC TGCAAACCAG AATGCGAGTC GAGCCTTGTG 
GAGCACCCGG TGTCCCGGCA GGTGTTCATC GTGCAGGATC TTGAAATTCG AGATCGTCTG 
GCAACGTCAC AAATGAATAA ATTTTTGTAC CTGTATTGCA GTAAAGAAAT GCCTCGGAAA 
GCTCATTCCA ACATGTTGAC AATTAAAGCA CTACACGTGC GTCCAGAGTC TGGCAGGTCC 
CCACAGGAGT GCTGCCTCAG AGTATCACTA ATGCCACTTC GCCTCAATAT TGACCAGGAT 
GCCTTGTTCT TCTTGAAGGA TTTCTTCACA AGTCTTTCTA CAGAAGTAGA GCTGCTCATG 
ACTCCAGATC CAGAAGTTAA AAAGTCTCCA GGGGCTGATG TCACCTGCAG TTTGCCAAGG 
CATTTAAGCG CCTCAAAAGA GCCAAATCTA GTTCTTTCTT TCCCTGGGCC AAAGCAGCCT 
TCCCCAAATG GCAGTGCCAA TTCAGTGCAA GCGGTTAATG GGGAAGAAGA GAAGGACTTC 
TCTCCTGAAG AAGCGTTGTT TAGTGATCAG CCTGTGTTTT TTAGAGAATT TAGATTCACA 
TCAGAAGTTC CTATTCGACT TGATTATCAT GGGAAACATG TATCAATGGA TCAGGGTACA 
TTAGCTGGGA TTTTGATTGG TCTCGCCCAG TTAAACTGCT CTGAACTAAA GCTGAAGAGG 
CTTTTCTATC GGCATGGTTT GCTAGGTGTG GACAAATTAT TCTCATACGC AATCACCGAG 
TGGCTTACTG ACATTAAGAA GAACCAGCTG CCAGGAATCC TGGGAGGTGT TGGGCCTATG 
CATTCACTAG TACAGCTAGT GCAAGGCCTC AAGGACTTGG TCTGGTTACC AATTGAGCAG 
TACCGAAAGG ACGGCCGCAT TGTCAGAGGG TTTCAGAGAG GGGCTGCCTC CTTTGGCACC 
TCGACAGCAA TGGCTGCTCT CGAGCTCACG AACAGAATGG TTCAAACCAT ACAGGCAGCA 
GCGGAGACTG CGTATGACAT GGTGTCTCCA GGCACCCTTT CTATCGAGCC CAAGAAGGTC 
AGGAGGTTCC CCCATCACCG GTTAGCCCAC CAGCCAGTGG ACCTGAGGGA AGGGGTGGCC 
AAGGCCTACA GTGTCGTGAA GGAGGGAATC GCAGACACGG CTCAGACCAT TTACGAAACT 
GCTGCTCGGG AACACGAGAG CCGGGGGGTG ACGGGCGCCG TGGGGGAAGT CCTGCGCCAG 
CTCCCCCCGG CCGTGGTGAA ACCTCTGATC GTCGCCACAG AAGCGACATC GAATGTCTTG 
GGTGGCATGA GAAACCAGAT CAGACCAGAT GTTCGGCAGG ACGAGTCGCA GAAGTGGCGC 
CATGGGGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837558.1
CDS	16..6270
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASASEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTVPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSISVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQAGQARLSSIPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWLEFSSGSLLCVTKYEGFDDKHYVCLHCGSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKNSQPPQVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDPIVIKENYFSLPVKKTDTSKAPLHFPIPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMTPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_024981790.1
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy related 2B (ATG2B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981790.1

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTCAATG ATTTGCTGCT CTGGGAACCA ACAGCTCCTT CGCCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCGTTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAACG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAGCAGC GGCTCGTTAC TTTGTGTGAC GAAGTATGAA 
GGCTTCGACG ACAAGCACTA CGTGTGCCTT CACTGCGGCA GTTTCAGCCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATATAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCACT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCTCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTCGCCTTGG ATAAATCATC TTCTACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCGC ACTTTGAATT GCACTGTTCG 
AGTGACGTCG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATATATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTGAA GAATTCTCAG CCCCCTCAGG TAGATTCTGT TGGTCGGTCA 
TCCTCACGTG GTCCAGTACT TCCTGAAGCA GATCAACAGA TATTACGAGA TTTGATGAGT 
GATGCTATGG AGGAGATTGA CATGCAAGAA GCAGCTTCAT CTGTAAAACC ACAGTCTAAT 
GGTGTTTCTG ATGAAAAATC TCAAGTTCAG GAACCATGTT GTTCAGACCT CTTCCTGTTT 
CCTGATGAGA GTGGAAACGT GTCCCAGGAG CCTGGCCCCA CTTACGCCTC ATTCACCCAC 
CACTTGATTA GTGATGCAGT GACAGGCGTG CCCACGGAAA ATGACGACTT TTGCATTCTT 
TTTGCACCAA GAGCAACTGT TCAGGAGAAG GAAGAAGAAC CAGTTGTAAA GATAATGGTT 
GATGATCCAA TTGTGATAAA AGAGAATTAT TTTAGTCTGC CTGTTAAGAA GACAGATACG 
AGCAAAGCCC CGTTACACTT TCCCATTCCT GTCGTTCGGT ACCTGGTTAA AGAAGTCTCT 
CTTGTTTGGC ATCTTTATGG AGGAAAAGAT TTTGGAACAG CCCCTCCTAC TTCTCCAGCT 
AAAAGTTACA TTAGTCCCCA TAGCTCACCT TCTCACACAC CTACAAGACA TGGACATAAT 
ATGGTTTGTG GGGGAAAAGG AAGAAACCAT GACTTTTTAA TGGAAATACA GTTAAGCAAG 
GTGAAGTTTC AGCACGAGGT GTACCCGCCC TGCAAACCAG AATGCGAGTC GAGCCTTGTG 
GAGCACCCGG TGTCCCGGCA GGTGTTCATC GTGCAGGATC TTGAAATTCG AGATCGTCTG 
GCAACGTCAC AAATGAATAA ATTTTTGTAC CTGTATTGCA GTAAAGAAAT GCCTCGGAAA 
GCTCATTCCA ACATGTTGAC AATTAAAGCA CTACACGTGC GTCCAGAGTC TGGCAGGTCC 
CCACAGGAGT GCTGCCTCAG AGTATCACTA ATGCCACTTC GCCTCAATAT TGACCAGGAT 
GCCTTGTTCT TCTTGAAGGA TTTCTTCACA AGTCTTTCTA CAGAAGTAGA GCTGCTCATG 
ACTCCAGATC CAGAAGTTAA AAAGTCTCCA GGGGCTGATG TCACCTGCAG TTTGCCAAGG 
CATTTAAGCG CCTCAAAAGA GCCAAATCTA GTTCTTTCTT TCCCTGGGCC AAAGCAGCCT 
TCCCCAAATG GCAGTGCCAA TTCAGTGCAA GCGGTTAATG GGGAAGAAGA GAAGGACTTC 
TCTCCTGAAG AAGCGTTGTT TAGTGATCAG CCTGTGTTTT TTAGAGAATT TAGATTCACA 
TCAGAAGTTC CTATTCGACT TGATTATCAT GGGAAACATG TATCAATGGA TCAGGGTACA 
TTAGCTGGGA TTTTGATTGG TCTCGCCCAG TTAAACTGCT CTGAACTAAA GCTGAAGAGG 
CTTTTCTATC GGCATGGTTT GCTAGGTGTG GACAAATTAT TCTCATACGC AATCACCGAG 
TGGCTTACTG ACATTAAGAA GAACCAGCTG CCAGGAATCC TGGGAGGTGT TGGGCCTATG 
CATTCACTAG TACAGCTAGT GCAAGGCCTC AAGGACTTGG TCTGGTTACC AATTGAGCAG 
TACCGAAAGG ACGGCCGCAT TGTCAGAGGG TTTCAGAGAG GGGCTGCCTC CTTTGGCACC 
TCGACAGCAA TGGCTGCTCT CGAGCTCACG AACAGAATGG TTCAAACCAT ACAGGCAGCA 
GCGGAGACTG CGTATGACAT GGTGTCTCCA GGCACCCTTT CTATCGAGCC CAAGAAGGTC 
AGGAGGTTCC CCCATCACCG GTTAGCCCAC CAGCCAGTGG ACCTGAGGGA AGGGGTGGCC 
AAGGCCTACA GTGTCGTGAA GGAGGGAATC GCAGACACGG CTCAGACCAT TTACGAAACT 
GCTGCTCGGG AACACGAGAG CCGGGGGGTG ACGGGCGCCG TGGGGGAAGT CCTGCGCCAG 
CTCCCCCCGG CCGTGGTGAA ACCTCTGATC GTCGCCACAG AAGCGACATC GAATGTCTTG 
GGTGGCATGA GAAACCAGAT CAGACCAGAT GTTCGGCAGG ACGAGTCGCA GAAGTGGCGC 
CATGGGGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_024981791.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837559.1 Bos taurus autophagy related 2B (ATG2B), transcript variant X5, mRNA.

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CloneID	OBa228332
Clone ID Related Accession (Same CDS sequence)	XM_024981791.1
Accession Version	XM_024981791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	autophagy-related protein 2 homolog B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTCAATG ATTTGCTGCT CTGGGAACCA ACAGCTCCTT CGCCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCGTTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAACG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAGCAGC GGCTCGTTAC TTTGTGTGAC GAAGTATGAA 
GGCTTCGACG ACAAGCACTA CGTGTGCCTT CACTGCGGCA GTTTCAGCCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATATAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCACT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCTCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTCGCCTTGG ATAAATCATC TTCTACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCGC ACTTTGAATT GCACTGTTCG 
AGTGACGTCG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATATATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTAGA TTCTGTTGGT CGGTCATCCT CACGTGGTCC AGTACTTCCT 
GAAGCAGATC AACAGATATT ACGAGATTTG ATGAGTGATG CTATGGAGGA GATTGACATG 
CAAGAAGCAG CTTCATCTGT AAAACCACAG TCTAATGGTG TTTCTGATGA AAAATCTCAA 
GTTCAGGAAC CATGTTGTTC AGACCTCTTC CTGTTTCCTG ATGAGAGTGG AAACGTGTCC 
CAGGAGCCTG GCCCCACTTA CGCCTCATTC ACCCACCACT TGATTAGTGA TGCAGTGACA 
GGCGTGCCCA CGGAAAATGA CGACTTTTGC ATTCTTTTTG CACCAAGAGC AACTGTTCAG 
GAGAAGGAAG AAGAACCAGT TGTAAAGATA ATGGTTGATG ATCCAATTGT GATAAAAGAG 
AATTATTTTA GTCTGCCTGT TAAGAAGACA GATACGAGCA AAGCCCCGTT ACACTTTCCC 
ATTCCTGTCG TTCGGTACCT GGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAAGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATTAG TCCCCATAGC 
TCACCTTCTC ACACACCTAC AAGACATGGA CATAATATGG TTTGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA CGAGGTGTAC 
CCGCCCTGCA AACCAGAATG CGAGTCGAGC CTTGTGGAGC ACCCGGTGTC CCGGCAGGTG 
TTCATCGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CGTCACAAAT GAATAAATTT 
TTGTACCTGT ATTGCAGTAA AGAAATGCCT CGGAAAGCTC ATTCCAACAT GTTGACAATT 
AAAGCACTAC ACGTGCGTCC AGAGTCTGGC AGGTCCCCAC AGGAGTGCTG CCTCAGAGTA 
TCACTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCTT GAAGGATTTC 
TTCACAAGTC TTTCTACAGA AGTAGAGCTG CTCATGACTC CAGATCCAGA AGTTAAAAAG 
TCTCCAGGGG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TAAGCGCCTC AAAAGAGCCA 
AATCTAGTTC TTTCTTTCCC TGGGCCAAAG CAGCCTTCCC CAAATGGCAG TGCCAATTCA 
GTGCAAGCGG TTAATGGGGA AGAAGAGAAG GACTTCTCTC CTGAAGAAGC GTTGTTTAGT 
GATCAGCCTG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTTCCTAT TCGACTTGAT 
TATCATGGGA AACATGTATC AATGGATCAG GGTACATTAG CTGGGATTTT GATTGGTCTC 
GCCCAGTTAA ACTGCTCTGA ACTAAAGCTG AAGAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTGTGGACA AATTATTCTC ATACGCAATC ACCGAGTGGC TTACTGACAT TAAGAAGAAC 
CAGCTGCCAG GAATCCTGGG AGGTGTTGGG CCTATGCATT CACTAGTACA GCTAGTGCAA 
GGCCTCAAGG ACTTGGTCTG GTTACCAATT GAGCAGTACC GAAAGGACGG CCGCATTGTC 
AGAGGGTTTC AGAGAGGGGC TGCCTCCTTT GGCACCTCGA CAGCAATGGC TGCTCTCGAG 
CTCACGAACA GAATGGTTCA AACCATACAG GCAGCAGCGG AGACTGCGTA TGACATGGTG 
TCTCCAGGCA CCCTTTCTAT CGAGCCCAAG AAGGTCAGGA GGTTCCCCCA TCACCGGTTA 
GCCCACCAGC CAGTGGACCT GAGGGAAGGG GTGGCCAAGG CCTACAGTGT CGTGAAGGAG 
GGAATCGCAG ACACGGCTCA GACCATTTAC GAAACTGCTG CTCGGGAACA CGAGAGCCGG 
GGGGTGACGG GCGCCGTGGG GGAAGTCCTG CGCCAGCTCC CCCCGGCCGT GGTGAAACCT 
CTGATCGTCG CCACAGAAGC GACATCGAAT GTCTTGGGTG GCATGAGAAA CCAGATCAGA 
CCAGATGTTC GGCAGGACGA GTCGCAGAAG TGGCGCCATG GGGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837559.1
CDS	16..6246
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASASEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTVPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSISVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQAGQARLSSIPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWLEFSSGSLLCVTKYEGFDDKHYVCLHCGSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDPIVIKENYFSLPVKKTDTSKAPLHFPIPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMTPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_024981791.1
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy related 2B (ATG2B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981791.1

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTCAATG ATTTGCTGCT CTGGGAACCA ACAGCTCCTT CGCCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCGTTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAACG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAGCAGC GGCTCGTTAC TTTGTGTGAC GAAGTATGAA 
GGCTTCGACG ACAAGCACTA CGTGTGCCTT CACTGCGGCA GTTTCAGCCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATATAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCACT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCTCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTCGCCTTGG ATAAATCATC TTCTACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCGC ACTTTGAATT GCACTGTTCG 
AGTGACGTCG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATATATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTAGA TTCTGTTGGT CGGTCATCCT CACGTGGTCC AGTACTTCCT 
GAAGCAGATC AACAGATATT ACGAGATTTG ATGAGTGATG CTATGGAGGA GATTGACATG 
CAAGAAGCAG CTTCATCTGT AAAACCACAG TCTAATGGTG TTTCTGATGA AAAATCTCAA 
GTTCAGGAAC CATGTTGTTC AGACCTCTTC CTGTTTCCTG ATGAGAGTGG AAACGTGTCC 
CAGGAGCCTG GCCCCACTTA CGCCTCATTC ACCCACCACT TGATTAGTGA TGCAGTGACA 
GGCGTGCCCA CGGAAAATGA CGACTTTTGC ATTCTTTTTG CACCAAGAGC AACTGTTCAG 
GAGAAGGAAG AAGAACCAGT TGTAAAGATA ATGGTTGATG ATCCAATTGT GATAAAAGAG 
AATTATTTTA GTCTGCCTGT TAAGAAGACA GATACGAGCA AAGCCCCGTT ACACTTTCCC 
ATTCCTGTCG TTCGGTACCT GGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAAGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATTAG TCCCCATAGC 
TCACCTTCTC ACACACCTAC AAGACATGGA CATAATATGG TTTGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA CGAGGTGTAC 
CCGCCCTGCA AACCAGAATG CGAGTCGAGC CTTGTGGAGC ACCCGGTGTC CCGGCAGGTG 
TTCATCGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CGTCACAAAT GAATAAATTT 
TTGTACCTGT ATTGCAGTAA AGAAATGCCT CGGAAAGCTC ATTCCAACAT GTTGACAATT 
AAAGCACTAC ACGTGCGTCC AGAGTCTGGC AGGTCCCCAC AGGAGTGCTG CCTCAGAGTA 
TCACTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCTT GAAGGATTTC 
TTCACAAGTC TTTCTACAGA AGTAGAGCTG CTCATGACTC CAGATCCAGA AGTTAAAAAG 
TCTCCAGGGG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TAAGCGCCTC AAAAGAGCCA 
AATCTAGTTC TTTCTTTCCC TGGGCCAAAG CAGCCTTCCC CAAATGGCAG TGCCAATTCA 
GTGCAAGCGG TTAATGGGGA AGAAGAGAAG GACTTCTCTC CTGAAGAAGC GTTGTTTAGT 
GATCAGCCTG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTTCCTAT TCGACTTGAT 
TATCATGGGA AACATGTATC AATGGATCAG GGTACATTAG CTGGGATTTT GATTGGTCTC 
GCCCAGTTAA ACTGCTCTGA ACTAAAGCTG AAGAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTGTGGACA AATTATTCTC ATACGCAATC ACCGAGTGGC TTACTGACAT TAAGAAGAAC 
CAGCTGCCAG GAATCCTGGG AGGTGTTGGG CCTATGCATT CACTAGTACA GCTAGTGCAA 
GGCCTCAAGG ACTTGGTCTG GTTACCAATT GAGCAGTACC GAAAGGACGG CCGCATTGTC 
AGAGGGTTTC AGAGAGGGGC TGCCTCCTTT GGCACCTCGA CAGCAATGGC TGCTCTCGAG 
CTCACGAACA GAATGGTTCA AACCATACAG GCAGCAGCGG AGACTGCGTA TGACATGGTG 
TCTCCAGGCA CCCTTTCTAT CGAGCCCAAG AAGGTCAGGA GGTTCCCCCA TCACCGGTTA 
GCCCACCAGC CAGTGGACCT GAGGGAAGGG GTGGCCAAGG CCTACAGTGT CGTGAAGGAG 
GGAATCGCAG ACACGGCTCA GACCATTTAC GAAACTGCTG CTCGGGAACA CGAGAGCCGG 
GGGGTGACGG GCGCCGTGGG GGAAGTCCTG CGCCAGCTCC CCCCGGCCGT GGTGAAACCT 
CTGATCGTCG CCACAGAAGC GACATCGAAT GTCTTGGGTG GCATGAGAAA CCAGATCAGA 
CCAGATGTTC GGCAGGACGA GTCGCAGAAG TGGCGCCATG GGGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_024981792.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837560.1 Bos taurus autophagy related 2B (ATG2B), transcript variant X6, mRNA.

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CloneID	OBa228333
Clone ID Related Accession (Same CDS sequence)	XM_024981792.1
Accession Version	XM_024981792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	autophagy-related protein 2 homolog B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTG CTTCTGAGCC CATGTATTGG TCAAGCTTCA TGACCAGCAG TATGCAATTG 
GCAAAAGAAT GTCTTAGCCA GAAACTAACA GATGAACAAG GAGAAGGATC TCAGCCTTTT 
GAAGGACTTG AAAAGTTTGC TGAAACCATC GAAACAGTAC TAAGAAGAGT AAAGGTCACT 
TTTATAGACA CTGTCTTGAG AATTGAACAC GTGCCAGAAA ATTCCAAGAC TGGAACTGCA 
CTTGAAATTC GAATAGAAAG AACCATGTAC TGTGATGAAA CTGCTGACGA GTCCTCAGGA 
ATTAACGTAC ATCAACCCAC AGCTTTTGCT CACAAGTTAC TTCAGCTCTC TGGAGTGTCT 
CTCTTCTGGG ATGAGTTTTC TGCATCTGCA AAATCCTCCC CAGTGTGTTC AACTGTGCCA 
GTGGAAACAG AGCCAAAGCT GTCACCTAGC TGGAATCCAA AAATTGTTTA TGAACCTCAC 
CCACAACTAA CAAGGAACTT GCCAGAAGTA GCACCTTCTG ACCCAGTGCA GATAGGAGGG 
CTCAATGGTA GGCTGGAGTT GAGTCTCACA TTGAAACAGA ATGAAGTACT TCCTGGAGCT 
AAGCTGGATA TTGATGGGCA GATAGACTCT ATACATCTGT TCCTGTCACC CAGGCAGGTG 
CACTTACTTT TGGACATGTT AGCAGCTATT GCTGGGCCAG AAAATTCAAG CAAAATTGGA 
TTAGCTAATA AAGATAGAAA AAACCGACCC ATGCAGCAAG AAGATGAATA TCGAATTCAA 
ATGGAATTGA ACCGGTATTA TTTGAGGAAA GACTCCCTCT CTGCCGGTGT GTCCTCAGAG 
CAAAGCTTTT ATGAGACAGA AACGGCTCGT ACACCTTCTA GCCGGGAAGA AGAAGTCTTC 
TTTTCCATGG CTGATATGGA CATGTCGCAC AGTCTCTCTT CTCTTCCACC CCTTGGGGAA 
CCCCCAAATA TGGACCTTGA GTTATCATTA ACTAGTACAT ATACAAACAC CCCAGCAGGA 
TCTCCATTAA GTACTACTGT GCTTCAGCCA ACATGGGGAG ACTTCCTTGA TCATCATAAG 
GAACAGCCAG TCAGAGGATC AGCGTTACCC TCCAACCTGG TTCACCCAGC ATCTTTACAA 
AAGACTGCTC TCCCCTCTAG GTCTGTTTCA GTGGATGAAT CCAGGCCTGA ACTTATTTTT 
AGAGTAGCTG TGGGAACGTT TTCAATCTCT GTGCTTCACA TTGATCCTTT ATCTCCACCT 
GAATCATCAC TGAATGTTAA TCCATTGACA CCAATGGCGA TAGCTTTCTT TACTTGTATA 
GAAAAGATTG ATCCAGCACA ATTTTCAACA GAAGATTTTA AGTCTTTCCG AGCAGTATTT 
GCAGAAGCTT GCTCACATGA TCACCTTAGA TTTATAGGCA CTGGCATCAA AGTGTCCTAT 
GAACAAAGAC AAAGATCGGC GTCCCGATAT TTCAGTACTG ATATGTCCAT TGGGCAAATG 
GAACTTTTGG AATGCCTGTT TCCAACTGAT TCTCATTCTG TTCCTCCTCA CTATACAGAG 
CTTTTGATGT TCCATTCCAA AGAACGAACT GATTCTCACC TCCCTGTGTG TCTTCAGCTT 
CATTATAAGC ATTCTGAGAA TAAAGGACCT CAGGCTGGTC AAGCAAGACT TAGTTCCATT 
CCTCAGAAGG CAGAATTACA GGTTAAGTTA AATCCAGTGT TTTGTGAGCT GGATATCAGT 
ATTGTGGACA GGTTAAATTC CTTGCTTCAA CCACAGAAAC TCACTACAGT GGAGATGATG 
GCATCGCATA TGTATACTTC ATATAATAAG CATATTAGTT TGCACAAAGC TTTCACTGAA 
GTATTTCTAG ATGATTCACA TAATCCTGCA AACTGCCGGG TATCAGTACA AGTTGCCACG 
CCAGCATTAA ACCTTTCTGT TCGCTTCCCA ATACCTGATC TTCGGTCAGA TCAAGAGAGA 
GGACCATGGT TTAAGAAGTC ACTTCAGAAG GAGATTCTTC ATTTAGCATT CACAGATCTA 
GAGTTTAAAA CTGAATTCCT AGGAGGATCA ACCCCTGAAC AAGTTAAATT GGAACTTACA 
TTTAGAGAAC TAGTCGGGTC ATTCCAGGAA GACAAAGGAG AACCATCTAT TAAGTTTTTC 
CATGTGGCTA GTGGAGGAGA TGGAGATACA ACGTCATCAG ATGACTTTGA CTGGCCGCGA 
ATTGTACTAA AAGTGAACCC ACCAGCCATG CATTCCATTT TGGAGAGAAT AGCAGCTGAG 
GAGGAAGAGG AAAGTGACGG CCACTATCAG GAGGAAGAGG AGGGAGGCGC TCATTCCCTG 
AAGGATGTCT GTGATCTGAG AAGGCCAGCT CCGTCTCCCT TCTCTTCCCG TAGAGTAATG 
TTTGAAAATG AACAGATGGT GATGCCAGGA GACCCTGTAG AAATGACAGA GTTTCAGAAT 
AAAGCAATCA GCAATTCTCA CTATGTGCTG GAACTTACAT TACCCAATAT TCATATAACA 
CTACCTAATA AAAGCTTTTA TGAGAAGCTT TATAATAGGG CCTCAGAAGC TCAGGAGTTG 
GCCCAGGAAG TTCCTAGACC AGTGGCTTCT CGCAGGAAGG GGCCAGGGTT TGCGTCATCG 
CTTGTCACCT TGCTGGCATT CACACTAGGC TTTACGATGA GCCTGTGCTT TTGGCAGTCC 
TTCATGATCT TCAATGATTT GCTGCTCTGG GAACCAACAG CTCCTTCGCC AGTAGAGACA 
TTTGAAAATA TTTCGTATGG TATTGGGCTT TCAGTAGCCA GTCAGCTGAT TAATACCTTC 
AGCAAGGATA GTTTTAGTCC GTTTAAATCT ACAGTTCACT ATGATGAGGA AAGTGGATCT 
GAGGAGGAGA CTTTGCAGTA TTTTTCTACT GTTGATCCCA ATTATCGTTC TCGTAGGAAA 
AAAAAACTAG ACTCCCAGAA CAAGAACTCA CAGAGTTTTC TCTCAGTTCT TCTGAGTATT 
AATCATGGGT TAATTGCAGT GTTTACAGAT GTAAAGCAAG ATAACGGAGA TCTGTTGGAA 
AACAAGCATG GTGAATTCTG GTTAGAGTTC AGCAGCGGCT CGTTACTTTG TGTGACGAAG 
TATGAAGGCT TCGACGACAA GCACTACGTG TGCCTTCACT GCGGCAGTTT CAGCCTCTAC 
CACAAAGGCA TAGTGGATGG AGCAGTTCCT TCTACAGAAA CACGACTGCC TAGTTCAACC 
CGCCCACATT GGTTAGAACC TACTATTTAT TCCTCTGAAG AAGATGGCCT CAGTCGAGCT 
TCTTCAGATG GTGTTGGAGG AGACACTTTG AATATGCTGT CTGTTGCAGT TAAAATAGTG 
TCTGATAAAT CAGAGTCCAA TACAAAGGAG TTTCTCATTG CTGTAGGGCT AAAAGGAGCT 
ACTCTCCAGC ATAGAATGCT TCCTTCTGGG CTTAGCTGGC ATGAGCAGAT TTTATACTTC 
TTGAATATTG CTGATGAACC TGTTCTGGGA TATAATCCTC CAACTTCATT TACAACCTTT 
CATGTTCATC TCTGGAGCTG TGCACTTGAT TATAGGCCTC TTTATTTGCC AATCCGATCT 
CTTCTTACTG TTGAAACATT CAGCGTTTCA AGTAGTGTCG CCTTGGATAA ATCATCTTCT 
ACTCTCAGAA TAATTTTGGA TGAAGCTGCT TTACACCTGT CTGACAAGTG TAATACTGTT 
ACTATAAATT TGAATAGAGA TTATGTTCGT GTGATGGATA TGGGACTTTT GGAATTAACC 
ATAACTGCAG TAAAGTCTGA TTCCGATGGA GAGCAAACTG AACCGCACTT TGAATTGCAC 
TGTTCGAGTG ACGTCGTCCA TGTTAGAACG TGCTCAGACT CTTGTGCTGC TCTAATGAAT 
CTAATTCAAT ATATTGCAAG CTATGGGGAC TTACATGCCA CTAACAAGGC AGACGTGAAG 
CCAGGAGCTC CTCAAAGAAA GGTGAAGAAT TCTCAGCCCC CTCAGGTAGA TTCTGTTGGT 
CGGTCATCCT CACGTGGTCC AGTACTTCCT GAAGCAGATC AACAGATATT ACGAGATTTG 
ATGAGTGATG CTATGGAGGA GATTGACATG CAAGAAGCAG CTTCATCTGT AAAACCACAG 
TCTAATGGTG TTTCTGATGA AAAATCTCAA GTTCAGGAAC CATGTTGTTC AGACCTCTTC 
CTGTTTCCTG ATGAGAGTGG AAACGTGTCC CAGGAGCCTG GCCCCACTTA CGCCTCATTC 
ACCCACCACT TGATTAGTGA TGCAGTGACA GGCGTGCCCA CGGAAAATGA CGACTTTTGC 
ATTCTTTTTG CACCAAGAGC AACTGTTCAG GAGAAGGAAG AAGAACCAGT TGTAAAGATA 
ATGGTTGATG ATCCAATTGT GATAAAAGAG AATTATTTTA GTCTGCCTGT TAAGAAGACA 
GATACGAGCA AAGCCCCGTT ACACTTTCCC ATTCCTGTCG TTCGGTACCT GGTTAAAGAA 
GTCTCTCTTG TTTGGCATCT TTATGGAGGA AAAGATTTTG GAACAGCCCC TCCTACTTCT 
CCAGCTAAAA GTTACATTAG TCCCCATAGC TCACCTTCTC ACACACCTAC AAGACATGGA 
CATAATATGG TTTGTGGGGG AAAAGGAAGA AACCATGACT TTTTAATGGA AATACAGTTA 
AGCAAGGTGA AGTTTCAGCA CGAGGTGTAC CCGCCCTGCA AACCAGAATG CGAGTCGAGC 
CTTGTGGAGC ACCCGGTGTC CCGGCAGGTG TTCATCGTGC AGGATCTTGA AATTCGAGAT 
CGTCTGGCAA CGTCACAAAT GAATAAATTT TTGTACCTGT ATTGCAGTAA AGAAATGCCT 
CGGAAAGCTC ATTCCAACAT GTTGACAATT AAAGCACTAC ACGTGCGTCC AGAGTCTGGC 
AGGTCCCCAC AGGAGTGCTG CCTCAGAGTA TCACTAATGC CACTTCGCCT CAATATTGAC 
CAGGATGCCT TGTTCTTCTT GAAGGATTTC TTCACAAGTC TTTCTACAGA AGTAGAGCTG 
CTCATGACTC CAGATCCAGA AGTTAAAAAG TCTCCAGGGG CTGATGTCAC CTGCAGTTTG 
CCAAGGCATT TAAGCGCCTC AAAAGAGCCA AATCTAGTTC TTTCTTTCCC TGGGCCAAAG 
CAGCCTTCCC CAAATGGCAG TGCCAATTCA GTGCAAGCGG TTAATGGGGA AGAAGAGAAG 
GACTTCTCTC CTGAAGAAGC GTTGTTTAGT GATCAGCCTG TGTTTTTTAG AGAATTTAGA 
TTCACATCAG AAGTTCCTAT TCGACTTGAT TATCATGGGA AACATGTATC AATGGATCAG 
GGTACATTAG CTGGGATTTT GATTGGTCTC GCCCAGTTAA ACTGCTCTGA ACTAAAGCTG 
AAGAGGCTTT TCTATCGGCA TGGTTTGCTA GGTGTGGACA AATTATTCTC ATACGCAATC 
ACCGAGTGGC TTACTGACAT TAAGAAGAAC CAGCTGCCAG GAATCCTGGG AGGTGTTGGG 
CCTATGCATT CACTAGTACA GCTAGTGCAA GGCCTCAAGG ACTTGGTCTG GTTACCAATT 
GAGCAGTACC GAAAGGACGG CCGCATTGTC AGAGGGTTTC AGAGAGGGGC TGCCTCCTTT 
GGCACCTCGA CAGCAATGGC TGCTCTCGAG CTCACGAACA GAATGGTTCA AACCATACAG 
GCAGCAGCGG AGACTGCGTA TGACATGGTG TCTCCAGGCA CCCTTTCTAT CGAGCCCAAG 
AAGGTCAGGA GGTTCCCCCA TCACCGGTTA GCCCACCAGC CAGTGGACCT GAGGGAAGGG 
GTGGCCAAGG CCTACAGTGT CGTGAAGGAG GGAATCGCAG ACACGGCTCA GACCATTTAC 
GAAACTGCTG CTCGGGAACA CGAGAGCCGG GGGGTGACGG GCGCCGTGGG GGAAGTCCTG 
CGCCAGCTCC CCCCGGCCGT GGTGAAACCT CTGATCGTCG CCACAGAAGC GACATCGAAT 
GTCTTGGGTG GCATGAGAAA CCAGATCAGA CCAGATGTTC GGCAGGACGA GTCGCAGAAG 
TGGCGCCATG GGGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837560.1
CDS	129..6209
Translation	MASASEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTVPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSISVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQAGQARLSSIPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRASEAQELAQEVPRPVASRRKGPGFASSLVTLLAFTLGFTMSLCFWQSFMIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWLEFSSGSLLCVTKYEGFDDKHYVCLHCGSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKNSQPPQVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDPIVIKENYFSLPVKKTDTSKAPLHFPIPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMTPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_024981792.1
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy related 2B (ATG2B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981792.1

ORF Insert Sequence:

ATGGCATCTG CTTCTGAGCC CATGTATTGG TCAAGCTTCA TGACCAGCAG TATGCAATTG 
GCAAAAGAAT GTCTTAGCCA GAAACTAACA GATGAACAAG GAGAAGGATC TCAGCCTTTT 
GAAGGACTTG AAAAGTTTGC TGAAACCATC GAAACAGTAC TAAGAAGAGT AAAGGTCACT 
TTTATAGACA CTGTCTTGAG AATTGAACAC GTGCCAGAAA ATTCCAAGAC TGGAACTGCA 
CTTGAAATTC GAATAGAAAG AACCATGTAC TGTGATGAAA CTGCTGACGA GTCCTCAGGA 
ATTAACGTAC ATCAACCCAC AGCTTTTGCT CACAAGTTAC TTCAGCTCTC TGGAGTGTCT 
CTCTTCTGGG ATGAGTTTTC TGCATCTGCA AAATCCTCCC CAGTGTGTTC AACTGTGCCA 
GTGGAAACAG AGCCAAAGCT GTCACCTAGC TGGAATCCAA AAATTGTTTA TGAACCTCAC 
CCACAACTAA CAAGGAACTT GCCAGAAGTA GCACCTTCTG ACCCAGTGCA GATAGGAGGG 
CTCAATGGTA GGCTGGAGTT GAGTCTCACA TTGAAACAGA ATGAAGTACT TCCTGGAGCT 
AAGCTGGATA TTGATGGGCA GATAGACTCT ATACATCTGT TCCTGTCACC CAGGCAGGTG 
CACTTACTTT TGGACATGTT AGCAGCTATT GCTGGGCCAG AAAATTCAAG CAAAATTGGA 
TTAGCTAATA AAGATAGAAA AAACCGACCC ATGCAGCAAG AAGATGAATA TCGAATTCAA 
ATGGAATTGA ACCGGTATTA TTTGAGGAAA GACTCCCTCT CTGCCGGTGT GTCCTCAGAG 
CAAAGCTTTT ATGAGACAGA AACGGCTCGT ACACCTTCTA GCCGGGAAGA AGAAGTCTTC 
TTTTCCATGG CTGATATGGA CATGTCGCAC AGTCTCTCTT CTCTTCCACC CCTTGGGGAA 
CCCCCAAATA TGGACCTTGA GTTATCATTA ACTAGTACAT ATACAAACAC CCCAGCAGGA 
TCTCCATTAA GTACTACTGT GCTTCAGCCA ACATGGGGAG ACTTCCTTGA TCATCATAAG 
GAACAGCCAG TCAGAGGATC AGCGTTACCC TCCAACCTGG TTCACCCAGC ATCTTTACAA 
AAGACTGCTC TCCCCTCTAG GTCTGTTTCA GTGGATGAAT CCAGGCCTGA ACTTATTTTT 
AGAGTAGCTG TGGGAACGTT TTCAATCTCT GTGCTTCACA TTGATCCTTT ATCTCCACCT 
GAATCATCAC TGAATGTTAA TCCATTGACA CCAATGGCGA TAGCTTTCTT TACTTGTATA 
GAAAAGATTG ATCCAGCACA ATTTTCAACA GAAGATTTTA AGTCTTTCCG AGCAGTATTT 
GCAGAAGCTT GCTCACATGA TCACCTTAGA TTTATAGGCA CTGGCATCAA AGTGTCCTAT 
GAACAAAGAC AAAGATCGGC GTCCCGATAT TTCAGTACTG ATATGTCCAT TGGGCAAATG 
GAACTTTTGG AATGCCTGTT TCCAACTGAT TCTCATTCTG TTCCTCCTCA CTATACAGAG 
CTTTTGATGT TCCATTCCAA AGAACGAACT GATTCTCACC TCCCTGTGTG TCTTCAGCTT 
CATTATAAGC ATTCTGAGAA TAAAGGACCT CAGGCTGGTC AAGCAAGACT TAGTTCCATT 
CCTCAGAAGG CAGAATTACA GGTTAAGTTA AATCCAGTGT TTTGTGAGCT GGATATCAGT 
ATTGTGGACA GGTTAAATTC CTTGCTTCAA CCACAGAAAC TCACTACAGT GGAGATGATG 
GCATCGCATA TGTATACTTC ATATAATAAG CATATTAGTT TGCACAAAGC TTTCACTGAA 
GTATTTCTAG ATGATTCACA TAATCCTGCA AACTGCCGGG TATCAGTACA AGTTGCCACG 
CCAGCATTAA ACCTTTCTGT TCGCTTCCCA ATACCTGATC TTCGGTCAGA TCAAGAGAGA 
GGACCATGGT TTAAGAAGTC ACTTCAGAAG GAGATTCTTC ATTTAGCATT CACAGATCTA 
GAGTTTAAAA CTGAATTCCT AGGAGGATCA ACCCCTGAAC AAGTTAAATT GGAACTTACA 
TTTAGAGAAC TAGTCGGGTC ATTCCAGGAA GACAAAGGAG AACCATCTAT TAAGTTTTTC 
CATGTGGCTA GTGGAGGAGA TGGAGATACA ACGTCATCAG ATGACTTTGA CTGGCCGCGA 
ATTGTACTAA AAGTGAACCC ACCAGCCATG CATTCCATTT TGGAGAGAAT AGCAGCTGAG 
GAGGAAGAGG AAAGTGACGG CCACTATCAG GAGGAAGAGG AGGGAGGCGC TCATTCCCTG 
AAGGATGTCT GTGATCTGAG AAGGCCAGCT CCGTCTCCCT TCTCTTCCCG TAGAGTAATG 
TTTGAAAATG AACAGATGGT GATGCCAGGA GACCCTGTAG AAATGACAGA GTTTCAGAAT 
AAAGCAATCA GCAATTCTCA CTATGTGCTG GAACTTACAT TACCCAATAT TCATATAACA 
CTACCTAATA AAAGCTTTTA TGAGAAGCTT TATAATAGGG CCTCAGAAGC TCAGGAGTTG 
GCCCAGGAAG TTCCTAGACC AGTGGCTTCT CGCAGGAAGG GGCCAGGGTT TGCGTCATCG 
CTTGTCACCT TGCTGGCATT CACACTAGGC TTTACGATGA GCCTGTGCTT TTGGCAGTCC 
TTCATGATCT TCAATGATTT GCTGCTCTGG GAACCAACAG CTCCTTCGCC AGTAGAGACA 
TTTGAAAATA TTTCGTATGG TATTGGGCTT TCAGTAGCCA GTCAGCTGAT TAATACCTTC 
AGCAAGGATA GTTTTAGTCC GTTTAAATCT ACAGTTCACT ATGATGAGGA AAGTGGATCT 
GAGGAGGAGA CTTTGCAGTA TTTTTCTACT GTTGATCCCA ATTATCGTTC TCGTAGGAAA 
AAAAAACTAG ACTCCCAGAA CAAGAACTCA CAGAGTTTTC TCTCAGTTCT TCTGAGTATT 
AATCATGGGT TAATTGCAGT GTTTACAGAT GTAAAGCAAG ATAACGGAGA TCTGTTGGAA 
AACAAGCATG GTGAATTCTG GTTAGAGTTC AGCAGCGGCT CGTTACTTTG TGTGACGAAG 
TATGAAGGCT TCGACGACAA GCACTACGTG TGCCTTCACT GCGGCAGTTT CAGCCTCTAC 
CACAAAGGCA TAGTGGATGG AGCAGTTCCT TCTACAGAAA CACGACTGCC TAGTTCAACC 
CGCCCACATT GGTTAGAACC TACTATTTAT TCCTCTGAAG AAGATGGCCT CAGTCGAGCT 
TCTTCAGATG GTGTTGGAGG AGACACTTTG AATATGCTGT CTGTTGCAGT TAAAATAGTG 
TCTGATAAAT CAGAGTCCAA TACAAAGGAG TTTCTCATTG CTGTAGGGCT AAAAGGAGCT 
ACTCTCCAGC ATAGAATGCT TCCTTCTGGG CTTAGCTGGC ATGAGCAGAT TTTATACTTC 
TTGAATATTG CTGATGAACC TGTTCTGGGA TATAATCCTC CAACTTCATT TACAACCTTT 
CATGTTCATC TCTGGAGCTG TGCACTTGAT TATAGGCCTC TTTATTTGCC AATCCGATCT 
CTTCTTACTG TTGAAACATT CAGCGTTTCA AGTAGTGTCG CCTTGGATAA ATCATCTTCT 
ACTCTCAGAA TAATTTTGGA TGAAGCTGCT TTACACCTGT CTGACAAGTG TAATACTGTT 
ACTATAAATT TGAATAGAGA TTATGTTCGT GTGATGGATA TGGGACTTTT GGAATTAACC 
ATAACTGCAG TAAAGTCTGA TTCCGATGGA GAGCAAACTG AACCGCACTT TGAATTGCAC 
TGTTCGAGTG ACGTCGTCCA TGTTAGAACG TGCTCAGACT CTTGTGCTGC TCTAATGAAT 
CTAATTCAAT ATATTGCAAG CTATGGGGAC TTACATGCCA CTAACAAGGC AGACGTGAAG 
CCAGGAGCTC CTCAAAGAAA GGTGAAGAAT TCTCAGCCCC CTCAGGTAGA TTCTGTTGGT 
CGGTCATCCT CACGTGGTCC AGTACTTCCT GAAGCAGATC AACAGATATT ACGAGATTTG 
ATGAGTGATG CTATGGAGGA GATTGACATG CAAGAAGCAG CTTCATCTGT AAAACCACAG 
TCTAATGGTG TTTCTGATGA AAAATCTCAA GTTCAGGAAC CATGTTGTTC AGACCTCTTC 
CTGTTTCCTG ATGAGAGTGG AAACGTGTCC CAGGAGCCTG GCCCCACTTA CGCCTCATTC 
ACCCACCACT TGATTAGTGA TGCAGTGACA GGCGTGCCCA CGGAAAATGA CGACTTTTGC 
ATTCTTTTTG CACCAAGAGC AACTGTTCAG GAGAAGGAAG AAGAACCAGT TGTAAAGATA 
ATGGTTGATG ATCCAATTGT GATAAAAGAG AATTATTTTA GTCTGCCTGT TAAGAAGACA 
GATACGAGCA AAGCCCCGTT ACACTTTCCC ATTCCTGTCG TTCGGTACCT GGTTAAAGAA 
GTCTCTCTTG TTTGGCATCT TTATGGAGGA AAAGATTTTG GAACAGCCCC TCCTACTTCT 
CCAGCTAAAA GTTACATTAG TCCCCATAGC TCACCTTCTC ACACACCTAC AAGACATGGA 
CATAATATGG TTTGTGGGGG AAAAGGAAGA AACCATGACT TTTTAATGGA AATACAGTTA 
AGCAAGGTGA AGTTTCAGCA CGAGGTGTAC CCGCCCTGCA AACCAGAATG CGAGTCGAGC 
CTTGTGGAGC ACCCGGTGTC CCGGCAGGTG TTCATCGTGC AGGATCTTGA AATTCGAGAT 
CGTCTGGCAA CGTCACAAAT GAATAAATTT TTGTACCTGT ATTGCAGTAA AGAAATGCCT 
CGGAAAGCTC ATTCCAACAT GTTGACAATT AAAGCACTAC ACGTGCGTCC AGAGTCTGGC 
AGGTCCCCAC AGGAGTGCTG CCTCAGAGTA TCACTAATGC CACTTCGCCT CAATATTGAC 
CAGGATGCCT TGTTCTTCTT GAAGGATTTC TTCACAAGTC TTTCTACAGA AGTAGAGCTG 
CTCATGACTC CAGATCCAGA AGTTAAAAAG TCTCCAGGGG CTGATGTCAC CTGCAGTTTG 
CCAAGGCATT TAAGCGCCTC AAAAGAGCCA AATCTAGTTC TTTCTTTCCC TGGGCCAAAG 
CAGCCTTCCC CAAATGGCAG TGCCAATTCA GTGCAAGCGG TTAATGGGGA AGAAGAGAAG 
GACTTCTCTC CTGAAGAAGC GTTGTTTAGT GATCAGCCTG TGTTTTTTAG AGAATTTAGA 
TTCACATCAG AAGTTCCTAT TCGACTTGAT TATCATGGGA AACATGTATC AATGGATCAG 
GGTACATTAG CTGGGATTTT GATTGGTCTC GCCCAGTTAA ACTGCTCTGA ACTAAAGCTG 
AAGAGGCTTT TCTATCGGCA TGGTTTGCTA GGTGTGGACA AATTATTCTC ATACGCAATC 
ACCGAGTGGC TTACTGACAT TAAGAAGAAC CAGCTGCCAG GAATCCTGGG AGGTGTTGGG 
CCTATGCATT CACTAGTACA GCTAGTGCAA GGCCTCAAGG ACTTGGTCTG GTTACCAATT 
GAGCAGTACC GAAAGGACGG CCGCATTGTC AGAGGGTTTC AGAGAGGGGC TGCCTCCTTT 
GGCACCTCGA CAGCAATGGC TGCTCTCGAG CTCACGAACA GAATGGTTCA AACCATACAG 
GCAGCAGCGG AGACTGCGTA TGACATGGTG TCTCCAGGCA CCCTTTCTAT CGAGCCCAAG 
AAGGTCAGGA GGTTCCCCCA TCACCGGTTA GCCCACCAGC CAGTGGACCT GAGGGAAGGG 
GTGGCCAAGG CCTACAGTGT CGTGAAGGAG GGAATCGCAG ACACGGCTCA GACCATTTAC 
GAAACTGCTG CTCGGGAACA CGAGAGCCGG GGGGTGACGG GCGCCGTGGG GGAAGTCCTG 
CGCCAGCTCC CCCCGGCCGT GGTGAAACCT CTGATCGTCG CCACAGAAGC GACATCGAAT 
GTCTTGGGTG GCATGAGAAA CCAGATCAGA CCAGATGTTC GGCAGGACGA GTCGCAGAAG 
TGGCGCCATG GGGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_005222199.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222256.1 Bos taurus autophagy related 2B (ATG2B), transcript variant X1, mRNA.

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CloneID	OBa31115
Clone ID Related Accession (Same CDS sequence)	XM_005222199.2 , XM_005222199.3
Accession Version	XM_005222199.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005222199.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
GCCTCAGAAG CTCAGGAGTT GGCCCAGGAA GTTCCTAGAC CAGTGGCTTC TCGCAGGAAG 
GGGCCAGGGT TTGCGTCATC GCTTGTCACC TTGCTGGCAT TCACACTAGG CTTTACGATG 
AGCCTGTGCT TTTGGCAGTC CTTCATGATC TTCAATGATT TGCTGCTCTG GGAACCAACA 
GCTCCTTCGC CAGTAGAGAC ATTTGAAAAT ATTTCGTATG GTATTGGGCT TTCAGTAGCC 
AGTCAGCTGA TTAATACCTT CAGCAAGGAT AGTTTTAGTC CGTTTAAATC TACAGTTCAC 
TATGATGAGG AAAGTGGATC TGAGGAGGAG ACTTTGCAGT ATTTTTCTAC TGTTGATCCC 
AATTATCGTT CTCGTAGGAA AAAAAAACTA GACTCCCAGA ACAAGAACTC ACAGAGTTTT 
CTCTCAGTTC TTCTGAGTAT TAATCATGGG TTAATTGCAG TGTTTACAGA TGTAAAGCAA 
GATAACGGAG ATCTGTTGGA AAACAAGCAT GGTGAATTCT GGTTAGAGTT CAGCAGCGGC 
TCGTTACTTT GTGTGACGAA GTATGAAGGC TTCGACGACA AGCACTACGT GTGCCTTCAC 
TGCGGCAGTT TCAGCCTCTA CCACAAAGGC ATAGTGGATG GAGCAGTTCC TTCTACAGAA 
ACACGACTGC CTAGTTCAAC CCGCCCACAT TGGTTAGAAC CTACTATTTA TTCCTCTGAA 
GAAGATGGCC TCAGTCGAGC TTCTTCAGAT GGTGTTGGAG GAGACACTTT GAATATGCTG 
TCTGTTGCAG TTAAAATAGT GTCTGATAAA TCAGAGTCCA ATACAAAGGA GTTTCTCATT 
GCTGTAGGGC TAAAAGGAGC TACTCTCCAG CATAGAATGC TTCCTTCTGG GCTTAGCTGG 
CATGAGCAGA TTTTATACTT CTTGAATATT GCTGATGAAC CTGTTCTGGG ATATAATCCT 
CCAACTTCAT TTACAACCTT TCATGTTCAT CTCTGGAGCT GTGCACTTGA TTATAGGCCT 
CTTTATTTGC CAATCCGATC TCTTCTTACT GTTGAAACAT TCAGCGTTTC AAGTAGTGTC 
GCCTTGGATA AATCATCTTC TACTCTCAGA ATAATTTTGG ATGAAGCTGC TTTACACCTG 
TCTGACAAGT GTAATACTGT TACTATAAAT TTGAATAGAG ATTATGTTCG TGTGATGGAT 
ATGGGACTTT TGGAATTAAC CATAACTGCA GTAAAGTCTG ATTCCGATGG AGAGCAAACT 
GAACCGCACT TTGAATTGCA CTGTTCGAGT GACGTCGTCC ATGTTAGAAC GTGCTCAGAC 
TCTTGTGCTG CTCTAATGAA TCTAATTCAA TATATTGCAA GCTATGGGGA CTTACATGCC 
ACTAACAAGG CAGACGTGAA GCCAGGAGCT CCTCAAAGAA AGGTGAAGGT AGATTCTGTT 
GGTCGGTCAT CCTCACGTGG TCCAGTACTT CCTGAAGCAG ATCAACAGAT ATTACGAGAT 
TTGATGAGTG ATGCTATGGA GGAGATTGAC ATGCAAGAAG CAGCTTCATC TGTAAAACCA 
CAGTCTAATG GTGTTTCTGA TGAAAAATCT CAAGTTCAGG AACCATGTTG TTCAGACCTC 
TTCCTGTTTC CTGATGAGAG TGGAAACGTG TCCCAGGAGC CTGGCCCCAC TTACGCCTCA 
TTCACCCACC ACTTGATTAG TGATGCAGTG ACAGGCGTGC CCACGGAAAA TGACGACTTT 
TGCATTCTTT TTGCACCAAG AGCAACTGTT CAGGAGAAGG AAGAAGAACC AGTTGTAAAG 
ATAATGGTTG ATGATCCAAT TGTGATAAAA GAGAATTATT TTAGTCTGCC TGTTAAGAAG 
ACAGATACGA GCAAAGCCCC GTTACACTTT CCCATTCCTG TCGTTCGGTA CCTGGTTAAA 
GAAGTCTCTC TTGTTTGGCA TCTTTATGGA GGAAAAGATT TTGGAACAGC CCCTCCTACT 
TCTCCAGCTA AAAGTTACAT TAGTCCCCAT AGCTCACCTT CTCACACACC TACAAGACAT 
GGACATAATA TGGTTTGTGG GGGAAAAGGA AGAAACCATG ACTTTTTAAT GGAAATACAG 
TTAAGCAAGG TGAAGTTTCA GCACGAGGTG TACCCGCCCT GCAAACCAGA ATGCGAGTCG 
AGCCTTGTGG AGCACCCGGT GTCCCGGCAG GTGTTCATCG TGCAGGATCT TGAAATTCGA 
GATCGTCTGG CAACGTCACA AATGAATAAA TTTTTGTACC TGTATTGCAG TAAAGAAATG 
CCTCGGAAAG CTCATTCCAA CATGTTGACA ATTAAAGCAC TACACGTGCG TCCAGAGTCT 
GGCAGGTCCC CACAGGAGTG CTGCCTCAGA GTATCACTAA TGCCACTTCG CCTCAATATT 
GACCAGGATG CCTTGTTCTT CTTGAAGGAT TTCTTCACAA GTCTTTCTAC AGAAGTAGAG 
CTGCTCATGA CTCCAGATCC AGAAGTTAAA AAGTCTCCAG GGGCTGATGT CACCTGCAGT 
TTGCCAAGGC ATTTAAGCGC CTCAAAAGAG CCAAATCTAG TTCTTTCTTT CCCTGGGCCA 
AAGCAGCCTT CCCCAAATGG CAGTGCCAAT TCAGTGCAAG CGGTTAATGG GGAAGAAGAG 
AAGGACTTCT CTCCTGAAGA AGCGTTGTTT AGTGATCAGC CTGTGTTTTT TAGAGAATTT 
AGATTCACAT CAGAAGTTCC TATTCGACTT GATTATCATG GGAAACATGT ATCAATGGAT 
CAGGGTACAT TAGCTGGGAT TTTGATTGGT CTCGCCCAGT TAAACTGCTC TGAACTAAAG 
CTGAAGAGGC TTTTCTATCG GCATGGTTTG CTAGGTGTGG ACAAATTATT CTCATACGCA 
ATCACCGAGT GGCTTACTGA CATTAAGAAG AACCAGCTGC CAGGAATCCT GGGAGGTGTT 
GGGCCTATGC ATTCACTAGT ACAGCTAGTG CAAGGCCTCA AGGACTTGGT CTGGTTACCA 
ATTGAGCAGT ACCGAAAGGA CGGCCGCATT GTCAGAGGGT TTCAGAGAGG GGCTGCCTCC 
TTTGGCACCT CGACAGCAAT GGCTGCTCTC GAGCTCACGA ACAGAATGGT TCAAACCATA 
CAGGCAGCAG CGGAGACTGC GTATGACATG GTGTCTCCAG GCACCCTTTC TATCGAGCCC 
AAGAAGGTCA GGAGGTTCCC CCATCACCGG TTAGCCCACC AGCCAGTGGA CCTGAGGGAA 
GGGGTGGCCA AGGCCTACAG TGTCGTGAAG GAGGGAATCG CAGACACGGC TCAGACCATT 
TACGAAACTG CTGCTCGGGA ACACGAGAGC CGGGGGGTGA CGGGCGCCGT GGGGGAAGTC 
CTGCGCCAGC TCCCCCCGGC CGTGGTGAAA CCTCTGATCG TCGCCACAGA AGCGACATCG 
AATGTCTTGG GTGGCATGAG AAACCAGATC AGACCAGATG TTCGGCAGGA CGAGTCGCAG 
AAGTGGCGCC ATGGGGACGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222256.1
CDS	2..6385
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASASEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTVPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSISVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQAGQARLSSIPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRASEAQELAQEVPRPVASRRKGPGFASSLVTLLAFTLGFTMSLCFWQSFMIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWLEFSSGSLLCVTKYEGFDDKHYVCLHCGSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDPIVIKENYFSLPVKKTDTSKAPLHFPIPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMTPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_005222199.2
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy related 2B (ATG2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222199.2

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
GCCTCAGAAG CTCAGGAGTT GGCCCAGGAA GTTCCTAGAC CAGTGGCTTC TCGCAGGAAG 
GGGCCAGGGT TTGCGTCATC GCTTGTCACC TTGCTGGCAT TCACACTAGG CTTTACGATG 
AGCCTGTGCT TTTGGCAGTC CTTCATGATC TTCAATGATT TGCTGCTCTG GGAACCAACA 
GCTCCTTCGC CAGTAGAGAC ATTTGAAAAT ATTTCGTATG GTATTGGGCT TTCAGTAGCC 
AGTCAGCTGA TTAATACCTT CAGCAAGGAT AGTTTTAGTC CGTTTAAATC TACAGTTCAC 
TATGATGAGG AAAGTGGATC TGAGGAGGAG ACTTTGCAGT ATTTTTCTAC TGTTGATCCC 
AATTATCGTT CTCGTAGGAA AAAAAAACTA GACTCCCAGA ACAAGAACTC ACAGAGTTTT 
CTCTCAGTTC TTCTGAGTAT TAATCATGGG TTAATTGCAG TGTTTACAGA TGTAAAGCAA 
GATAACGGAG ATCTGTTGGA AAACAAGCAT GGTGAATTCT GGTTAGAGTT CAGCAGCGGC 
TCGTTACTTT GTGTGACGAA GTATGAAGGC TTCGACGACA AGCACTACGT GTGCCTTCAC 
TGCGGCAGTT TCAGCCTCTA CCACAAAGGC ATAGTGGATG GAGCAGTTCC TTCTACAGAA 
ACACGACTGC CTAGTTCAAC CCGCCCACAT TGGTTAGAAC CTACTATTTA TTCCTCTGAA 
GAAGATGGCC TCAGTCGAGC TTCTTCAGAT GGTGTTGGAG GAGACACTTT GAATATGCTG 
TCTGTTGCAG TTAAAATAGT GTCTGATAAA TCAGAGTCCA ATACAAAGGA GTTTCTCATT 
GCTGTAGGGC TAAAAGGAGC TACTCTCCAG CATAGAATGC TTCCTTCTGG GCTTAGCTGG 
CATGAGCAGA TTTTATACTT CTTGAATATT GCTGATGAAC CTGTTCTGGG ATATAATCCT 
CCAACTTCAT TTACAACCTT TCATGTTCAT CTCTGGAGCT GTGCACTTGA TTATAGGCCT 
CTTTATTTGC CAATCCGATC TCTTCTTACT GTTGAAACAT TCAGCGTTTC AAGTAGTGTC 
GCCTTGGATA AATCATCTTC TACTCTCAGA ATAATTTTGG ATGAAGCTGC TTTACACCTG 
TCTGACAAGT GTAATACTGT TACTATAAAT TTGAATAGAG ATTATGTTCG TGTGATGGAT 
ATGGGACTTT TGGAATTAAC CATAACTGCA GTAAAGTCTG ATTCCGATGG AGAGCAAACT 
GAACCGCACT TTGAATTGCA CTGTTCGAGT GACGTCGTCC ATGTTAGAAC GTGCTCAGAC 
TCTTGTGCTG CTCTAATGAA TCTAATTCAA TATATTGCAA GCTATGGGGA CTTACATGCC 
ACTAACAAGG CAGACGTGAA GCCAGGAGCT CCTCAAAGAA AGGTGAAGGT AGATTCTGTT 
GGTCGGTCAT CCTCACGTGG TCCAGTACTT CCTGAAGCAG ATCAACAGAT ATTACGAGAT 
TTGATGAGTG ATGCTATGGA GGAGATTGAC ATGCAAGAAG CAGCTTCATC TGTAAAACCA 
CAGTCTAATG GTGTTTCTGA TGAAAAATCT CAAGTTCAGG AACCATGTTG TTCAGACCTC 
TTCCTGTTTC CTGATGAGAG TGGAAACGTG TCCCAGGAGC CTGGCCCCAC TTACGCCTCA 
TTCACCCACC ACTTGATTAG TGATGCAGTG ACAGGCGTGC CCACGGAAAA TGACGACTTT 
TGCATTCTTT TTGCACCAAG AGCAACTGTT CAGGAGAAGG AAGAAGAACC AGTTGTAAAG 
ATAATGGTTG ATGATCCAAT TGTGATAAAA GAGAATTATT TTAGTCTGCC TGTTAAGAAG 
ACAGATACGA GCAAAGCCCC GTTACACTTT CCCATTCCTG TCGTTCGGTA CCTGGTTAAA 
GAAGTCTCTC TTGTTTGGCA TCTTTATGGA GGAAAAGATT TTGGAACAGC CCCTCCTACT 
TCTCCAGCTA AAAGTTACAT TAGTCCCCAT AGCTCACCTT CTCACACACC TACAAGACAT 
GGACATAATA TGGTTTGTGG GGGAAAAGGA AGAAACCATG ACTTTTTAAT GGAAATACAG 
TTAAGCAAGG TGAAGTTTCA GCACGAGGTG TACCCGCCCT GCAAACCAGA ATGCGAGTCG 
AGCCTTGTGG AGCACCCGGT GTCCCGGCAG GTGTTCATCG TGCAGGATCT TGAAATTCGA 
GATCGTCTGG CAACGTCACA AATGAATAAA TTTTTGTACC TGTATTGCAG TAAAGAAATG 
CCTCGGAAAG CTCATTCCAA CATGTTGACA ATTAAAGCAC TACACGTGCG TCCAGAGTCT 
GGCAGGTCCC CACAGGAGTG CTGCCTCAGA GTATCACTAA TGCCACTTCG CCTCAATATT 
GACCAGGATG CCTTGTTCTT CTTGAAGGAT TTCTTCACAA GTCTTTCTAC AGAAGTAGAG 
CTGCTCATGA CTCCAGATCC AGAAGTTAAA AAGTCTCCAG GGGCTGATGT CACCTGCAGT 
TTGCCAAGGC ATTTAAGCGC CTCAAAAGAG CCAAATCTAG TTCTTTCTTT CCCTGGGCCA 
AAGCAGCCTT CCCCAAATGG CAGTGCCAAT TCAGTGCAAG CGGTTAATGG GGAAGAAGAG 
AAGGACTTCT CTCCTGAAGA AGCGTTGTTT AGTGATCAGC CTGTGTTTTT TAGAGAATTT 
AGATTCACAT CAGAAGTTCC TATTCGACTT GATTATCATG GGAAACATGT ATCAATGGAT 
CAGGGTACAT TAGCTGGGAT TTTGATTGGT CTCGCCCAGT TAAACTGCTC TGAACTAAAG 
CTGAAGAGGC TTTTCTATCG GCATGGTTTG CTAGGTGTGG ACAAATTATT CTCATACGCA 
ATCACCGAGT GGCTTACTGA CATTAAGAAG AACCAGCTGC CAGGAATCCT GGGAGGTGTT 
GGGCCTATGC ATTCACTAGT ACAGCTAGTG CAAGGCCTCA AGGACTTGGT CTGGTTACCA 
ATTGAGCAGT ACCGAAAGGA CGGCCGCATT GTCAGAGGGT TTCAGAGAGG GGCTGCCTCC 
TTTGGCACCT CGACAGCAAT GGCTGCTCTC GAGCTCACGA ACAGAATGGT TCAAACCATA 
CAGGCAGCAG CGGAGACTGC GTATGACATG GTGTCTCCAG GCACCCTTTC TATCGAGCCC 
AAGAAGGTCA GGAGGTTCCC CCATCACCGG TTAGCCCACC AGCCAGTGGA CCTGAGGGAA 
GGGGTGGCCA AGGCCTACAG TGTCGTGAAG GAGGGAATCG CAGACACGGC TCAGACCATT 
TACGAAACTG CTGCTCGGGA ACACGAGAGC CGGGGGGTGA CGGGCGCCGT GGGGGAAGTC 
CTGCGCCAGC TCCCCCCGGC CGTGGTGAAA CCTCTGATCG TCGCCACAGA AGCGACATCG 
AATGTCTTGG GTGGCATGAG AAACCAGATC AGACCAGATG TTCGGCAGGA CGAGTCGCAG 
AAGTGGCGCC ATGGGGACGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_005222199.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222256.1 Bos taurus autophagy related 2B (ATG2B), transcript variant X2, mRNA.

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CloneID	OBa31115
Clone ID Related Accession (Same CDS sequence)	XM_005222199.2 , XM_005222199.3
Accession Version	XM_005222199.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222199.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
GCCTCAGAAG CTCAGGAGTT GGCCCAGGAA GTTCCTAGAC CAGTGGCTTC TCGCAGGAAG 
GGGCCAGGGT TTGCGTCATC GCTTGTCACC TTGCTGGCAT TCACACTAGG CTTTACGATG 
AGCCTGTGCT TTTGGCAGTC CTTCATGATC TTCAATGATT TGCTGCTCTG GGAACCAACA 
GCTCCTTCGC CAGTAGAGAC ATTTGAAAAT ATTTCGTATG GTATTGGGCT TTCAGTAGCC 
AGTCAGCTGA TTAATACCTT CAGCAAGGAT AGTTTTAGTC CGTTTAAATC TACAGTTCAC 
TATGATGAGG AAAGTGGATC TGAGGAGGAG ACTTTGCAGT ATTTTTCTAC TGTTGATCCC 
AATTATCGTT CTCGTAGGAA AAAAAAACTA GACTCCCAGA ACAAGAACTC ACAGAGTTTT 
CTCTCAGTTC TTCTGAGTAT TAATCATGGG TTAATTGCAG TGTTTACAGA TGTAAAGCAA 
GATAACGGAG ATCTGTTGGA AAACAAGCAT GGTGAATTCT GGTTAGAGTT CAGCAGCGGC 
TCGTTACTTT GTGTGACGAA GTATGAAGGC TTCGACGACA AGCACTACGT GTGCCTTCAC 
TGCGGCAGTT TCAGCCTCTA CCACAAAGGC ATAGTGGATG GAGCAGTTCC TTCTACAGAA 
ACACGACTGC CTAGTTCAAC CCGCCCACAT TGGTTAGAAC CTACTATTTA TTCCTCTGAA 
GAAGATGGCC TCAGTCGAGC TTCTTCAGAT GGTGTTGGAG GAGACACTTT GAATATGCTG 
TCTGTTGCAG TTAAAATAGT GTCTGATAAA TCAGAGTCCA ATACAAAGGA GTTTCTCATT 
GCTGTAGGGC TAAAAGGAGC TACTCTCCAG CATAGAATGC TTCCTTCTGG GCTTAGCTGG 
CATGAGCAGA TTTTATACTT CTTGAATATT GCTGATGAAC CTGTTCTGGG ATATAATCCT 
CCAACTTCAT TTACAACCTT TCATGTTCAT CTCTGGAGCT GTGCACTTGA TTATAGGCCT 
CTTTATTTGC CAATCCGATC TCTTCTTACT GTTGAAACAT TCAGCGTTTC AAGTAGTGTC 
GCCTTGGATA AATCATCTTC TACTCTCAGA ATAATTTTGG ATGAAGCTGC TTTACACCTG 
TCTGACAAGT GTAATACTGT TACTATAAAT TTGAATAGAG ATTATGTTCG TGTGATGGAT 
ATGGGACTTT TGGAATTAAC CATAACTGCA GTAAAGTCTG ATTCCGATGG AGAGCAAACT 
GAACCGCACT TTGAATTGCA CTGTTCGAGT GACGTCGTCC ATGTTAGAAC GTGCTCAGAC 
TCTTGTGCTG CTCTAATGAA TCTAATTCAA TATATTGCAA GCTATGGGGA CTTACATGCC 
ACTAACAAGG CAGACGTGAA GCCAGGAGCT CCTCAAAGAA AGGTGAAGGT AGATTCTGTT 
GGTCGGTCAT CCTCACGTGG TCCAGTACTT CCTGAAGCAG ATCAACAGAT ATTACGAGAT 
TTGATGAGTG ATGCTATGGA GGAGATTGAC ATGCAAGAAG CAGCTTCATC TGTAAAACCA 
CAGTCTAATG GTGTTTCTGA TGAAAAATCT CAAGTTCAGG AACCATGTTG TTCAGACCTC 
TTCCTGTTTC CTGATGAGAG TGGAAACGTG TCCCAGGAGC CTGGCCCCAC TTACGCCTCA 
TTCACCCACC ACTTGATTAG TGATGCAGTG ACAGGCGTGC CCACGGAAAA TGACGACTTT 
TGCATTCTTT TTGCACCAAG AGCAACTGTT CAGGAGAAGG AAGAAGAACC AGTTGTAAAG 
ATAATGGTTG ATGATCCAAT TGTGATAAAA GAGAATTATT TTAGTCTGCC TGTTAAGAAG 
ACAGATACGA GCAAAGCCCC GTTACACTTT CCCATTCCTG TCGTTCGGTA CCTGGTTAAA 
GAAGTCTCTC TTGTTTGGCA TCTTTATGGA GGAAAAGATT TTGGAACAGC CCCTCCTACT 
TCTCCAGCTA AAAGTTACAT TAGTCCCCAT AGCTCACCTT CTCACACACC TACAAGACAT 
GGACATAATA TGGTTTGTGG GGGAAAAGGA AGAAACCATG ACTTTTTAAT GGAAATACAG 
TTAAGCAAGG TGAAGTTTCA GCACGAGGTG TACCCGCCCT GCAAACCAGA ATGCGAGTCG 
AGCCTTGTGG AGCACCCGGT GTCCCGGCAG GTGTTCATCG TGCAGGATCT TGAAATTCGA 
GATCGTCTGG CAACGTCACA AATGAATAAA TTTTTGTACC TGTATTGCAG TAAAGAAATG 
CCTCGGAAAG CTCATTCCAA CATGTTGACA ATTAAAGCAC TACACGTGCG TCCAGAGTCT 
GGCAGGTCCC CACAGGAGTG CTGCCTCAGA GTATCACTAA TGCCACTTCG CCTCAATATT 
GACCAGGATG CCTTGTTCTT CTTGAAGGAT TTCTTCACAA GTCTTTCTAC AGAAGTAGAG 
CTGCTCATGA CTCCAGATCC AGAAGTTAAA AAGTCTCCAG GGGCTGATGT CACCTGCAGT 
TTGCCAAGGC ATTTAAGCGC CTCAAAAGAG CCAAATCTAG TTCTTTCTTT CCCTGGGCCA 
AAGCAGCCTT CCCCAAATGG CAGTGCCAAT TCAGTGCAAG CGGTTAATGG GGAAGAAGAG 
AAGGACTTCT CTCCTGAAGA AGCGTTGTTT AGTGATCAGC CTGTGTTTTT TAGAGAATTT 
AGATTCACAT CAGAAGTTCC TATTCGACTT GATTATCATG GGAAACATGT ATCAATGGAT 
CAGGGTACAT TAGCTGGGAT TTTGATTGGT CTCGCCCAGT TAAACTGCTC TGAACTAAAG 
CTGAAGAGGC TTTTCTATCG GCATGGTTTG CTAGGTGTGG ACAAATTATT CTCATACGCA 
ATCACCGAGT GGCTTACTGA CATTAAGAAG AACCAGCTGC CAGGAATCCT GGGAGGTGTT 
GGGCCTATGC ATTCACTAGT ACAGCTAGTG CAAGGCCTCA AGGACTTGGT CTGGTTACCA 
ATTGAGCAGT ACCGAAAGGA CGGCCGCATT GTCAGAGGGT TTCAGAGAGG GGCTGCCTCC 
TTTGGCACCT CGACAGCAAT GGCTGCTCTC GAGCTCACGA ACAGAATGGT TCAAACCATA 
CAGGCAGCAG CGGAGACTGC GTATGACATG GTGTCTCCAG GCACCCTTTC TATCGAGCCC 
AAGAAGGTCA GGAGGTTCCC CCATCACCGG TTAGCCCACC AGCCAGTGGA CCTGAGGGAA 
GGGGTGGCCA AGGCCTACAG TGTCGTGAAG GAGGGAATCG CAGACACGGC TCAGACCATT 
TACGAAACTG CTGCTCGGGA ACACGAGAGC CGGGGGGTGA CGGGCGCCGT GGGGGAAGTC 
CTGCGCCAGC TCCCCCCGGC CGTGGTGAAA CCTCTGATCG TCGCCACAGA AGCGACATCG 
AATGTCTTGG GTGGCATGAG AAACCAGATC AGACCAGATG TTCGGCAGGA CGAGTCGCAG 
AAGTGGCGCC ATGGGGACGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222256.1
CDS	16..6399
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASASEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTVPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSISVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQAGQARLSSIPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRASEAQELAQEVPRPVASRRKGPGFASSLVTLLAFTLGFTMSLCFWQSFMIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWLEFSSGSLLCVTKYEGFDDKHYVCLHCGSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDPIVIKENYFSLPVKKTDTSKAPLHFPIPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMTPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_005222199.3
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy related 2B (ATG2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222199.3

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
GCCTCAGAAG CTCAGGAGTT GGCCCAGGAA GTTCCTAGAC CAGTGGCTTC TCGCAGGAAG 
GGGCCAGGGT TTGCGTCATC GCTTGTCACC TTGCTGGCAT TCACACTAGG CTTTACGATG 
AGCCTGTGCT TTTGGCAGTC CTTCATGATC TTCAATGATT TGCTGCTCTG GGAACCAACA 
GCTCCTTCGC CAGTAGAGAC ATTTGAAAAT ATTTCGTATG GTATTGGGCT TTCAGTAGCC 
AGTCAGCTGA TTAATACCTT CAGCAAGGAT AGTTTTAGTC CGTTTAAATC TACAGTTCAC 
TATGATGAGG AAAGTGGATC TGAGGAGGAG ACTTTGCAGT ATTTTTCTAC TGTTGATCCC 
AATTATCGTT CTCGTAGGAA AAAAAAACTA GACTCCCAGA ACAAGAACTC ACAGAGTTTT 
CTCTCAGTTC TTCTGAGTAT TAATCATGGG TTAATTGCAG TGTTTACAGA TGTAAAGCAA 
GATAACGGAG ATCTGTTGGA AAACAAGCAT GGTGAATTCT GGTTAGAGTT CAGCAGCGGC 
TCGTTACTTT GTGTGACGAA GTATGAAGGC TTCGACGACA AGCACTACGT GTGCCTTCAC 
TGCGGCAGTT TCAGCCTCTA CCACAAAGGC ATAGTGGATG GAGCAGTTCC TTCTACAGAA 
ACACGACTGC CTAGTTCAAC CCGCCCACAT TGGTTAGAAC CTACTATTTA TTCCTCTGAA 
GAAGATGGCC TCAGTCGAGC TTCTTCAGAT GGTGTTGGAG GAGACACTTT GAATATGCTG 
TCTGTTGCAG TTAAAATAGT GTCTGATAAA TCAGAGTCCA ATACAAAGGA GTTTCTCATT 
GCTGTAGGGC TAAAAGGAGC TACTCTCCAG CATAGAATGC TTCCTTCTGG GCTTAGCTGG 
CATGAGCAGA TTTTATACTT CTTGAATATT GCTGATGAAC CTGTTCTGGG ATATAATCCT 
CCAACTTCAT TTACAACCTT TCATGTTCAT CTCTGGAGCT GTGCACTTGA TTATAGGCCT 
CTTTATTTGC CAATCCGATC TCTTCTTACT GTTGAAACAT TCAGCGTTTC AAGTAGTGTC 
GCCTTGGATA AATCATCTTC TACTCTCAGA ATAATTTTGG ATGAAGCTGC TTTACACCTG 
TCTGACAAGT GTAATACTGT TACTATAAAT TTGAATAGAG ATTATGTTCG TGTGATGGAT 
ATGGGACTTT TGGAATTAAC CATAACTGCA GTAAAGTCTG ATTCCGATGG AGAGCAAACT 
GAACCGCACT TTGAATTGCA CTGTTCGAGT GACGTCGTCC ATGTTAGAAC GTGCTCAGAC 
TCTTGTGCTG CTCTAATGAA TCTAATTCAA TATATTGCAA GCTATGGGGA CTTACATGCC 
ACTAACAAGG CAGACGTGAA GCCAGGAGCT CCTCAAAGAA AGGTGAAGGT AGATTCTGTT 
GGTCGGTCAT CCTCACGTGG TCCAGTACTT CCTGAAGCAG ATCAACAGAT ATTACGAGAT 
TTGATGAGTG ATGCTATGGA GGAGATTGAC ATGCAAGAAG CAGCTTCATC TGTAAAACCA 
CAGTCTAATG GTGTTTCTGA TGAAAAATCT CAAGTTCAGG AACCATGTTG TTCAGACCTC 
TTCCTGTTTC CTGATGAGAG TGGAAACGTG TCCCAGGAGC CTGGCCCCAC TTACGCCTCA 
TTCACCCACC ACTTGATTAG TGATGCAGTG ACAGGCGTGC CCACGGAAAA TGACGACTTT 
TGCATTCTTT TTGCACCAAG AGCAACTGTT CAGGAGAAGG AAGAAGAACC AGTTGTAAAG 
ATAATGGTTG ATGATCCAAT TGTGATAAAA GAGAATTATT TTAGTCTGCC TGTTAAGAAG 
ACAGATACGA GCAAAGCCCC GTTACACTTT CCCATTCCTG TCGTTCGGTA CCTGGTTAAA 
GAAGTCTCTC TTGTTTGGCA TCTTTATGGA GGAAAAGATT TTGGAACAGC CCCTCCTACT 
TCTCCAGCTA AAAGTTACAT TAGTCCCCAT AGCTCACCTT CTCACACACC TACAAGACAT 
GGACATAATA TGGTTTGTGG GGGAAAAGGA AGAAACCATG ACTTTTTAAT GGAAATACAG 
TTAAGCAAGG TGAAGTTTCA GCACGAGGTG TACCCGCCCT GCAAACCAGA ATGCGAGTCG 
AGCCTTGTGG AGCACCCGGT GTCCCGGCAG GTGTTCATCG TGCAGGATCT TGAAATTCGA 
GATCGTCTGG CAACGTCACA AATGAATAAA TTTTTGTACC TGTATTGCAG TAAAGAAATG 
CCTCGGAAAG CTCATTCCAA CATGTTGACA ATTAAAGCAC TACACGTGCG TCCAGAGTCT 
GGCAGGTCCC CACAGGAGTG CTGCCTCAGA GTATCACTAA TGCCACTTCG CCTCAATATT 
GACCAGGATG CCTTGTTCTT CTTGAAGGAT TTCTTCACAA GTCTTTCTAC AGAAGTAGAG 
CTGCTCATGA CTCCAGATCC AGAAGTTAAA AAGTCTCCAG GGGCTGATGT CACCTGCAGT 
TTGCCAAGGC ATTTAAGCGC CTCAAAAGAG CCAAATCTAG TTCTTTCTTT CCCTGGGCCA 
AAGCAGCCTT CCCCAAATGG CAGTGCCAAT TCAGTGCAAG CGGTTAATGG GGAAGAAGAG 
AAGGACTTCT CTCCTGAAGA AGCGTTGTTT AGTGATCAGC CTGTGTTTTT TAGAGAATTT 
AGATTCACAT CAGAAGTTCC TATTCGACTT GATTATCATG GGAAACATGT ATCAATGGAT 
CAGGGTACAT TAGCTGGGAT TTTGATTGGT CTCGCCCAGT TAAACTGCTC TGAACTAAAG 
CTGAAGAGGC TTTTCTATCG GCATGGTTTG CTAGGTGTGG ACAAATTATT CTCATACGCA 
ATCACCGAGT GGCTTACTGA CATTAAGAAG AACCAGCTGC CAGGAATCCT GGGAGGTGTT 
GGGCCTATGC ATTCACTAGT ACAGCTAGTG CAAGGCCTCA AGGACTTGGT CTGGTTACCA 
ATTGAGCAGT ACCGAAAGGA CGGCCGCATT GTCAGAGGGT TTCAGAGAGG GGCTGCCTCC 
TTTGGCACCT CGACAGCAAT GGCTGCTCTC GAGCTCACGA ACAGAATGGT TCAAACCATA 
CAGGCAGCAG CGGAGACTGC GTATGACATG GTGTCTCCAG GCACCCTTTC TATCGAGCCC 
AAGAAGGTCA GGAGGTTCCC CCATCACCGG TTAGCCCACC AGCCAGTGGA CCTGAGGGAA 
GGGGTGGCCA AGGCCTACAG TGTCGTGAAG GAGGGAATCG CAGACACGGC TCAGACCATT 
TACGAAACTG CTGCTCGGGA ACACGAGAGC CGGGGGGTGA CGGGCGCCGT GGGGGAAGTC 
CTGCGCCAGC TCCCCCCGGC CGTGGTGAAA CCTCTGATCG TCGCCACAGA AGCGACATCG 
AATGTCTTGG GTGGCATGAG AAACCAGATC AGACCAGATG TTCGGCAGGA CGAGTCGCAG 
AAGTGGCGCC ATGGGGACGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_005222200.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222257.1 Bos taurus autophagy related 2B (ATG2B), transcript variant X2, mRNA.

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CloneID	OBa31116
Clone ID Related Accession (Same CDS sequence)	XM_005222200.3 , XM_005222200.4
Accession Version	XM_005222200.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222200.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
GCCTCAGAAG CTCAGGAGTT GGCCCAGGAA GTTCCTAGAC CAGTGGCTTC TCGCAGGAAG 
GGGCCAGGGT TTGCGTCATC GCTTGTCACC TTGCTGGCAT TCACACTAGG CTTTACGATG 
AGCCTGTGCT TTTGGCAGTC CTTCATGATC TTCAATGATT TGCTGCTCTG GGAACCAACA 
GCTCCTTCGC CAGTAGAGAC ATTTGAAAAT ATTTCGTATG GTATTGGGCT TTCAGTAGCC 
AGTCAGCTGA TTAATACCTT CAGCAAGGAT AGTTTTAGTC CGTTTAAATC TACAGTTCAC 
TATGATGAGG AAAGTGGATC TGAGGAGGAG ACTTTGCAGT ATTTTTCTAC TGTTGATCCC 
AATTATCGTT CTCGTAGGAA AAAAAAACTA GACTCCCAGA ACAAGAACTC ACAGAGTTTT 
CTCTCAGTTC TTCTGAGTAT TAATCATGGG TTAATTGCAG TGTTTACAGA TGTAAAGCAA 
GATAACGGAG ATCTGTTGGA AAACAAGCAT GGTGAATTCT GGTTAGAGTT CAGCAGCGGC 
TCGTTACTTT GTGTGACGAA GTATGAAGGC TTCGACGACA AGCACTACGT GTGCCTTCAC 
TGCGGCAGTT TCAGCCTCTA CCACAAAGGC ATAGTGGATG GAGCAGTTCC TTCTACAGAA 
ACACGACTGC CTAGTTCAAC CCGCCCACAT TGGTTAGAAC CTACTATTTA TTCCTCTGAA 
GAAGATGGCC TCAGTCGAGC TTCTTCAGAT GGTGTTGGAG GAGACACTTT GAATATGCTG 
TCTGTTGCAG TTAAAATAGT GTCTGATAAA TCAGAGTCCA ATACAAAGGA GTTTCTCATT 
GCTGTAGGGC TAAAAGGAGC TACTCTCCAG CATAGAATGC TTCCTTCTGG GCTTAGCTGG 
CATGAGCAGA TTTTATACTT CTTGAATATT GCTGATGAAC CTGTTCTGGG ATATAATCCT 
CCAACTTCAT TTACAACCTT TCATGTTCAT CTCTGGAGCT GTGCACTTGA TTATAGGCCT 
CTTTATTTGC CAATCCGATC TCTTCTTACT GTTGAAACAT TCAGCGTTTC AAGTAGTGTC 
GCCTTGGATA AATCATCTTC TACTCTCAGA ATAATTTTGG ATGAAGCTGC TTTACACCTG 
TCTGACAAGT GTAATACTGT TACTATAAAT TTGAATAGAG ATTATGTTCG TGTGATGGAT 
ATGGGACTTT TGGAATTAAC CATAACTGCA GTAAAGTCTG ATTCCGATGG AGAGCAAACT 
GAACCGCACT TTGAATTGCA CTGTTCGAGT GACGTCGTCC ATGTTAGAAC GTGCTCAGAC 
TCTTGTGCTG CTCTAATGAA TCTAATTCAA TATATTGCAA GCTATGGGGA CTTACATGCC 
ACTAACAAGG CAGACGTGAA GCCAGGAGCT CCTCAAAGAA AGGTAGATTC TGTTGGTCGG 
TCATCCTCAC GTGGTCCAGT ACTTCCTGAA GCAGATCAAC AGATATTACG AGATTTGATG 
AGTGATGCTA TGGAGGAGAT TGACATGCAA GAAGCAGCTT CATCTGTAAA ACCACAGTCT 
AATGGTGTTT CTGATGAAAA ATCTCAAGTT CAGGAACCAT GTTGTTCAGA CCTCTTCCTG 
TTTCCTGATG AGAGTGGAAA CGTGTCCCAG GAGCCTGGCC CCACTTACGC CTCATTCACC 
CACCACTTGA TTAGTGATGC AGTGACAGGC GTGCCCACGG AAAATGACGA CTTTTGCATT 
CTTTTTGCAC CAAGAGCAAC TGTTCAGGAG AAGGAAGAAG AACCAGTTGT AAAGATAATG 
GTTGATGATC CAATTGTGAT AAAAGAGAAT TATTTTAGTC TGCCTGTTAA GAAGACAGAT 
ACGAGCAAAG CCCCGTTACA CTTTCCCATT CCTGTCGTTC GGTACCTGGT TAAAGAAGTC 
TCTCTTGTTT GGCATCTTTA TGGAGGAAAA GATTTTGGAA CAGCCCCTCC TACTTCTCCA 
GCTAAAAGTT ACATTAGTCC CCATAGCTCA CCTTCTCACA CACCTACAAG ACATGGACAT 
AATATGGTTT GTGGGGGAAA AGGAAGAAAC CATGACTTTT TAATGGAAAT ACAGTTAAGC 
AAGGTGAAGT TTCAGCACGA GGTGTACCCG CCCTGCAAAC CAGAATGCGA GTCGAGCCTT 
GTGGAGCACC CGGTGTCCCG GCAGGTGTTC ATCGTGCAGG ATCTTGAAAT TCGAGATCGT 
CTGGCAACGT CACAAATGAA TAAATTTTTG TACCTGTATT GCAGTAAAGA AATGCCTCGG 
AAAGCTCATT CCAACATGTT GACAATTAAA GCACTACACG TGCGTCCAGA GTCTGGCAGG 
TCCCCACAGG AGTGCTGCCT CAGAGTATCA CTAATGCCAC TTCGCCTCAA TATTGACCAG 
GATGCCTTGT TCTTCTTGAA GGATTTCTTC ACAAGTCTTT CTACAGAAGT AGAGCTGCTC 
ATGACTCCAG ATCCAGAAGT TAAAAAGTCT CCAGGGGCTG ATGTCACCTG CAGTTTGCCA 
AGGCATTTAA GCGCCTCAAA AGAGCCAAAT CTAGTTCTTT CTTTCCCTGG GCCAAAGCAG 
CCTTCCCCAA ATGGCAGTGC CAATTCAGTG CAAGCGGTTA ATGGGGAAGA AGAGAAGGAC 
TTCTCTCCTG AAGAAGCGTT GTTTAGTGAT CAGCCTGTGT TTTTTAGAGA ATTTAGATTC 
ACATCAGAAG TTCCTATTCG ACTTGATTAT CATGGGAAAC ATGTATCAAT GGATCAGGGT 
ACATTAGCTG GGATTTTGAT TGGTCTCGCC CAGTTAAACT GCTCTGAACT AAAGCTGAAG 
AGGCTTTTCT ATCGGCATGG TTTGCTAGGT GTGGACAAAT TATTCTCATA CGCAATCACC 
GAGTGGCTTA CTGACATTAA GAAGAACCAG CTGCCAGGAA TCCTGGGAGG TGTTGGGCCT 
ATGCATTCAC TAGTACAGCT AGTGCAAGGC CTCAAGGACT TGGTCTGGTT ACCAATTGAG 
CAGTACCGAA AGGACGGCCG CATTGTCAGA GGGTTTCAGA GAGGGGCTGC CTCCTTTGGC 
ACCTCGACAG CAATGGCTGC TCTCGAGCTC ACGAACAGAA TGGTTCAAAC CATACAGGCA 
GCAGCGGAGA CTGCGTATGA CATGGTGTCT CCAGGCACCC TTTCTATCGA GCCCAAGAAG 
GTCAGGAGGT TCCCCCATCA CCGGTTAGCC CACCAGCCAG TGGACCTGAG GGAAGGGGTG 
GCCAAGGCCT ACAGTGTCGT GAAGGAGGGA ATCGCAGACA CGGCTCAGAC CATTTACGAA 
ACTGCTGCTC GGGAACACGA GAGCCGGGGG GTGACGGGCG CCGTGGGGGA AGTCCTGCGC 
CAGCTCCCCC CGGCCGTGGT GAAACCTCTG ATCGTCGCCA CAGAAGCGAC ATCGAATGTC 
TTGGGTGGCA TGAGAAACCA GATCAGACCA GATGTTCGGC AGGACGAGTC GCAGAAGTGG 
CGCCATGGGG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222257.1
CDS	2..6379
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASASEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTVPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSISVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQAGQARLSSIPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRASEAQELAQEVPRPVASRRKGPGFASSLVTLLAFTLGFTMSLCFWQSFMIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWLEFSSGSLLCVTKYEGFDDKHYVCLHCGSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDPIVIKENYFSLPVKKTDTSKAPLHFPIPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMTPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_005222200.3
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy related 2B (ATG2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222200.3

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
GCCTCAGAAG CTCAGGAGTT GGCCCAGGAA GTTCCTAGAC CAGTGGCTTC TCGCAGGAAG 
GGGCCAGGGT TTGCGTCATC GCTTGTCACC TTGCTGGCAT TCACACTAGG CTTTACGATG 
AGCCTGTGCT TTTGGCAGTC CTTCATGATC TTCAATGATT TGCTGCTCTG GGAACCAACA 
GCTCCTTCGC CAGTAGAGAC ATTTGAAAAT ATTTCGTATG GTATTGGGCT TTCAGTAGCC 
AGTCAGCTGA TTAATACCTT CAGCAAGGAT AGTTTTAGTC CGTTTAAATC TACAGTTCAC 
TATGATGAGG AAAGTGGATC TGAGGAGGAG ACTTTGCAGT ATTTTTCTAC TGTTGATCCC 
AATTATCGTT CTCGTAGGAA AAAAAAACTA GACTCCCAGA ACAAGAACTC ACAGAGTTTT 
CTCTCAGTTC TTCTGAGTAT TAATCATGGG TTAATTGCAG TGTTTACAGA TGTAAAGCAA 
GATAACGGAG ATCTGTTGGA AAACAAGCAT GGTGAATTCT GGTTAGAGTT CAGCAGCGGC 
TCGTTACTTT GTGTGACGAA GTATGAAGGC TTCGACGACA AGCACTACGT GTGCCTTCAC 
TGCGGCAGTT TCAGCCTCTA CCACAAAGGC ATAGTGGATG GAGCAGTTCC TTCTACAGAA 
ACACGACTGC CTAGTTCAAC CCGCCCACAT TGGTTAGAAC CTACTATTTA TTCCTCTGAA 
GAAGATGGCC TCAGTCGAGC TTCTTCAGAT GGTGTTGGAG GAGACACTTT GAATATGCTG 
TCTGTTGCAG TTAAAATAGT GTCTGATAAA TCAGAGTCCA ATACAAAGGA GTTTCTCATT 
GCTGTAGGGC TAAAAGGAGC TACTCTCCAG CATAGAATGC TTCCTTCTGG GCTTAGCTGG 
CATGAGCAGA TTTTATACTT CTTGAATATT GCTGATGAAC CTGTTCTGGG ATATAATCCT 
CCAACTTCAT TTACAACCTT TCATGTTCAT CTCTGGAGCT GTGCACTTGA TTATAGGCCT 
CTTTATTTGC CAATCCGATC TCTTCTTACT GTTGAAACAT TCAGCGTTTC AAGTAGTGTC 
GCCTTGGATA AATCATCTTC TACTCTCAGA ATAATTTTGG ATGAAGCTGC TTTACACCTG 
TCTGACAAGT GTAATACTGT TACTATAAAT TTGAATAGAG ATTATGTTCG TGTGATGGAT 
ATGGGACTTT TGGAATTAAC CATAACTGCA GTAAAGTCTG ATTCCGATGG AGAGCAAACT 
GAACCGCACT TTGAATTGCA CTGTTCGAGT GACGTCGTCC ATGTTAGAAC GTGCTCAGAC 
TCTTGTGCTG CTCTAATGAA TCTAATTCAA TATATTGCAA GCTATGGGGA CTTACATGCC 
ACTAACAAGG CAGACGTGAA GCCAGGAGCT CCTCAAAGAA AGGTAGATTC TGTTGGTCGG 
TCATCCTCAC GTGGTCCAGT ACTTCCTGAA GCAGATCAAC AGATATTACG AGATTTGATG 
AGTGATGCTA TGGAGGAGAT TGACATGCAA GAAGCAGCTT CATCTGTAAA ACCACAGTCT 
AATGGTGTTT CTGATGAAAA ATCTCAAGTT CAGGAACCAT GTTGTTCAGA CCTCTTCCTG 
TTTCCTGATG AGAGTGGAAA CGTGTCCCAG GAGCCTGGCC CCACTTACGC CTCATTCACC 
CACCACTTGA TTAGTGATGC AGTGACAGGC GTGCCCACGG AAAATGACGA CTTTTGCATT 
CTTTTTGCAC CAAGAGCAAC TGTTCAGGAG AAGGAAGAAG AACCAGTTGT AAAGATAATG 
GTTGATGATC CAATTGTGAT AAAAGAGAAT TATTTTAGTC TGCCTGTTAA GAAGACAGAT 
ACGAGCAAAG CCCCGTTACA CTTTCCCATT CCTGTCGTTC GGTACCTGGT TAAAGAAGTC 
TCTCTTGTTT GGCATCTTTA TGGAGGAAAA GATTTTGGAA CAGCCCCTCC TACTTCTCCA 
GCTAAAAGTT ACATTAGTCC CCATAGCTCA CCTTCTCACA CACCTACAAG ACATGGACAT 
AATATGGTTT GTGGGGGAAA AGGAAGAAAC CATGACTTTT TAATGGAAAT ACAGTTAAGC 
AAGGTGAAGT TTCAGCACGA GGTGTACCCG CCCTGCAAAC CAGAATGCGA GTCGAGCCTT 
GTGGAGCACC CGGTGTCCCG GCAGGTGTTC ATCGTGCAGG ATCTTGAAAT TCGAGATCGT 
CTGGCAACGT CACAAATGAA TAAATTTTTG TACCTGTATT GCAGTAAAGA AATGCCTCGG 
AAAGCTCATT CCAACATGTT GACAATTAAA GCACTACACG TGCGTCCAGA GTCTGGCAGG 
TCCCCACAGG AGTGCTGCCT CAGAGTATCA CTAATGCCAC TTCGCCTCAA TATTGACCAG 
GATGCCTTGT TCTTCTTGAA GGATTTCTTC ACAAGTCTTT CTACAGAAGT AGAGCTGCTC 
ATGACTCCAG ATCCAGAAGT TAAAAAGTCT CCAGGGGCTG ATGTCACCTG CAGTTTGCCA 
AGGCATTTAA GCGCCTCAAA AGAGCCAAAT CTAGTTCTTT CTTTCCCTGG GCCAAAGCAG 
CCTTCCCCAA ATGGCAGTGC CAATTCAGTG CAAGCGGTTA ATGGGGAAGA AGAGAAGGAC 
TTCTCTCCTG AAGAAGCGTT GTTTAGTGAT CAGCCTGTGT TTTTTAGAGA ATTTAGATTC 
ACATCAGAAG TTCCTATTCG ACTTGATTAT CATGGGAAAC ATGTATCAAT GGATCAGGGT 
ACATTAGCTG GGATTTTGAT TGGTCTCGCC CAGTTAAACT GCTCTGAACT AAAGCTGAAG 
AGGCTTTTCT ATCGGCATGG TTTGCTAGGT GTGGACAAAT TATTCTCATA CGCAATCACC 
GAGTGGCTTA CTGACATTAA GAAGAACCAG CTGCCAGGAA TCCTGGGAGG TGTTGGGCCT 
ATGCATTCAC TAGTACAGCT AGTGCAAGGC CTCAAGGACT TGGTCTGGTT ACCAATTGAG 
CAGTACCGAA AGGACGGCCG CATTGTCAGA GGGTTTCAGA GAGGGGCTGC CTCCTTTGGC 
ACCTCGACAG CAATGGCTGC TCTCGAGCTC ACGAACAGAA TGGTTCAAAC CATACAGGCA 
GCAGCGGAGA CTGCGTATGA CATGGTGTCT CCAGGCACCC TTTCTATCGA GCCCAAGAAG 
GTCAGGAGGT TCCCCCATCA CCGGTTAGCC CACCAGCCAG TGGACCTGAG GGAAGGGGTG 
GCCAAGGCCT ACAGTGTCGT GAAGGAGGGA ATCGCAGACA CGGCTCAGAC CATTTACGAA 
ACTGCTGCTC GGGAACACGA GAGCCGGGGG GTGACGGGCG CCGTGGGGGA AGTCCTGCGC 
CAGCTCCCCC CGGCCGTGGT GAAACCTCTG ATCGTCGCCA CAGAAGCGAC ATCGAATGTC 
TTGGGTGGCA TGAGAAACCA GATCAGACCA GATGTTCGGC AGGACGAGTC GCAGAAGTGG 
CGCCATGGGG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_005222200.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222257.1 Bos taurus autophagy related 2B (ATG2B), transcript variant X3, mRNA.

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CloneID	OBa31116
Clone ID Related Accession (Same CDS sequence)	XM_005222200.3 , XM_005222200.4
Accession Version	XM_005222200.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	autophagy-related protein 2 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222200.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
GCCTCAGAAG CTCAGGAGTT GGCCCAGGAA GTTCCTAGAC CAGTGGCTTC TCGCAGGAAG 
GGGCCAGGGT TTGCGTCATC GCTTGTCACC TTGCTGGCAT TCACACTAGG CTTTACGATG 
AGCCTGTGCT TTTGGCAGTC CTTCATGATC TTCAATGATT TGCTGCTCTG GGAACCAACA 
GCTCCTTCGC CAGTAGAGAC ATTTGAAAAT ATTTCGTATG GTATTGGGCT TTCAGTAGCC 
AGTCAGCTGA TTAATACCTT CAGCAAGGAT AGTTTTAGTC CGTTTAAATC TACAGTTCAC 
TATGATGAGG AAAGTGGATC TGAGGAGGAG ACTTTGCAGT ATTTTTCTAC TGTTGATCCC 
AATTATCGTT CTCGTAGGAA AAAAAAACTA GACTCCCAGA ACAAGAACTC ACAGAGTTTT 
CTCTCAGTTC TTCTGAGTAT TAATCATGGG TTAATTGCAG TGTTTACAGA TGTAAAGCAA 
GATAACGGAG ATCTGTTGGA AAACAAGCAT GGTGAATTCT GGTTAGAGTT CAGCAGCGGC 
TCGTTACTTT GTGTGACGAA GTATGAAGGC TTCGACGACA AGCACTACGT GTGCCTTCAC 
TGCGGCAGTT TCAGCCTCTA CCACAAAGGC ATAGTGGATG GAGCAGTTCC TTCTACAGAA 
ACACGACTGC CTAGTTCAAC CCGCCCACAT TGGTTAGAAC CTACTATTTA TTCCTCTGAA 
GAAGATGGCC TCAGTCGAGC TTCTTCAGAT GGTGTTGGAG GAGACACTTT GAATATGCTG 
TCTGTTGCAG TTAAAATAGT GTCTGATAAA TCAGAGTCCA ATACAAAGGA GTTTCTCATT 
GCTGTAGGGC TAAAAGGAGC TACTCTCCAG CATAGAATGC TTCCTTCTGG GCTTAGCTGG 
CATGAGCAGA TTTTATACTT CTTGAATATT GCTGATGAAC CTGTTCTGGG ATATAATCCT 
CCAACTTCAT TTACAACCTT TCATGTTCAT CTCTGGAGCT GTGCACTTGA TTATAGGCCT 
CTTTATTTGC CAATCCGATC TCTTCTTACT GTTGAAACAT TCAGCGTTTC AAGTAGTGTC 
GCCTTGGATA AATCATCTTC TACTCTCAGA ATAATTTTGG ATGAAGCTGC TTTACACCTG 
TCTGACAAGT GTAATACTGT TACTATAAAT TTGAATAGAG ATTATGTTCG TGTGATGGAT 
ATGGGACTTT TGGAATTAAC CATAACTGCA GTAAAGTCTG ATTCCGATGG AGAGCAAACT 
GAACCGCACT TTGAATTGCA CTGTTCGAGT GACGTCGTCC ATGTTAGAAC GTGCTCAGAC 
TCTTGTGCTG CTCTAATGAA TCTAATTCAA TATATTGCAA GCTATGGGGA CTTACATGCC 
ACTAACAAGG CAGACGTGAA GCCAGGAGCT CCTCAAAGAA AGGTAGATTC TGTTGGTCGG 
TCATCCTCAC GTGGTCCAGT ACTTCCTGAA GCAGATCAAC AGATATTACG AGATTTGATG 
AGTGATGCTA TGGAGGAGAT TGACATGCAA GAAGCAGCTT CATCTGTAAA ACCACAGTCT 
AATGGTGTTT CTGATGAAAA ATCTCAAGTT CAGGAACCAT GTTGTTCAGA CCTCTTCCTG 
TTTCCTGATG AGAGTGGAAA CGTGTCCCAG GAGCCTGGCC CCACTTACGC CTCATTCACC 
CACCACTTGA TTAGTGATGC AGTGACAGGC GTGCCCACGG AAAATGACGA CTTTTGCATT 
CTTTTTGCAC CAAGAGCAAC TGTTCAGGAG AAGGAAGAAG AACCAGTTGT AAAGATAATG 
GTTGATGATC CAATTGTGAT AAAAGAGAAT TATTTTAGTC TGCCTGTTAA GAAGACAGAT 
ACGAGCAAAG CCCCGTTACA CTTTCCCATT CCTGTCGTTC GGTACCTGGT TAAAGAAGTC 
TCTCTTGTTT GGCATCTTTA TGGAGGAAAA GATTTTGGAA CAGCCCCTCC TACTTCTCCA 
GCTAAAAGTT ACATTAGTCC CCATAGCTCA CCTTCTCACA CACCTACAAG ACATGGACAT 
AATATGGTTT GTGGGGGAAA AGGAAGAAAC CATGACTTTT TAATGGAAAT ACAGTTAAGC 
AAGGTGAAGT TTCAGCACGA GGTGTACCCG CCCTGCAAAC CAGAATGCGA GTCGAGCCTT 
GTGGAGCACC CGGTGTCCCG GCAGGTGTTC ATCGTGCAGG ATCTTGAAAT TCGAGATCGT 
CTGGCAACGT CACAAATGAA TAAATTTTTG TACCTGTATT GCAGTAAAGA AATGCCTCGG 
AAAGCTCATT CCAACATGTT GACAATTAAA GCACTACACG TGCGTCCAGA GTCTGGCAGG 
TCCCCACAGG AGTGCTGCCT CAGAGTATCA CTAATGCCAC TTCGCCTCAA TATTGACCAG 
GATGCCTTGT TCTTCTTGAA GGATTTCTTC ACAAGTCTTT CTACAGAAGT AGAGCTGCTC 
ATGACTCCAG ATCCAGAAGT TAAAAAGTCT CCAGGGGCTG ATGTCACCTG CAGTTTGCCA 
AGGCATTTAA GCGCCTCAAA AGAGCCAAAT CTAGTTCTTT CTTTCCCTGG GCCAAAGCAG 
CCTTCCCCAA ATGGCAGTGC CAATTCAGTG CAAGCGGTTA ATGGGGAAGA AGAGAAGGAC 
TTCTCTCCTG AAGAAGCGTT GTTTAGTGAT CAGCCTGTGT TTTTTAGAGA ATTTAGATTC 
ACATCAGAAG TTCCTATTCG ACTTGATTAT CATGGGAAAC ATGTATCAAT GGATCAGGGT 
ACATTAGCTG GGATTTTGAT TGGTCTCGCC CAGTTAAACT GCTCTGAACT AAAGCTGAAG 
AGGCTTTTCT ATCGGCATGG TTTGCTAGGT GTGGACAAAT TATTCTCATA CGCAATCACC 
GAGTGGCTTA CTGACATTAA GAAGAACCAG CTGCCAGGAA TCCTGGGAGG TGTTGGGCCT 
ATGCATTCAC TAGTACAGCT AGTGCAAGGC CTCAAGGACT TGGTCTGGTT ACCAATTGAG 
CAGTACCGAA AGGACGGCCG CATTGTCAGA GGGTTTCAGA GAGGGGCTGC CTCCTTTGGC 
ACCTCGACAG CAATGGCTGC TCTCGAGCTC ACGAACAGAA TGGTTCAAAC CATACAGGCA 
GCAGCGGAGA CTGCGTATGA CATGGTGTCT CCAGGCACCC TTTCTATCGA GCCCAAGAAG 
GTCAGGAGGT TCCCCCATCA CCGGTTAGCC CACCAGCCAG TGGACCTGAG GGAAGGGGTG 
GCCAAGGCCT ACAGTGTCGT GAAGGAGGGA ATCGCAGACA CGGCTCAGAC CATTTACGAA 
ACTGCTGCTC GGGAACACGA GAGCCGGGGG GTGACGGGCG CCGTGGGGGA AGTCCTGCGC 
CAGCTCCCCC CGGCCGTGGT GAAACCTCTG ATCGTCGCCA CAGAAGCGAC ATCGAATGTC 
TTGGGTGGCA TGAGAAACCA GATCAGACCA GATGTTCGGC AGGACGAGTC GCAGAAGTGG 
CGCCATGGGG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222257.1
CDS	16..6393
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASASEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTVPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSISVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQAGQARLSSIPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRASEAQELAQEVPRPVASRRKGPGFASSLVTLLAFTLGFTMSLCFWQSFMIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWLEFSSGSLLCVTKYEGFDDKHYVCLHCGSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDPIVIKENYFSLPVKKTDTSKAPLHFPIPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMTPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_005222200.4
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy related 2B (ATG2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222200.4

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACA GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGGGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCATCT GCTTCTGAGC CCATGTATTG GTCAAGCTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAGGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA CGTGCCAGAA 
AATTCCAAGA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAACGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGTGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAACCTCA CCCACAACTA ACAAGGAACT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGCTGGAT ATTGATGGGC AGATAGACTC TATACATCTG 
TTCCTGTCAC CCAGGCAGGT GCACTTACTT TTGGACATGT TAGCAGCTAT TGCTGGGCCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGGTATT ATTTGAGGAA AGACTCCCTC 
TCTGCCGGTG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTCTT CTTTTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA GGAACAGCCA GTCAGAGGAT CAGCGTTACC CTCCAACCTG 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTCCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCGG CGTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCCTGT TTCCAACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGCTGGT 
CAAGCAAGAC TTAGTTCCAT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTCACTACAG TGGAGATGAT GGCATCGCAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGATTCAC ATAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC GCCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAACTTAC ATTTAGAGAA CTAGTCGGGT CATTCCAGGA AGACAAAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTA AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGACG GCCACTATCA GGAGGAAGAG 
GAGGGAGGCG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC TCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTACCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
GCCTCAGAAG CTCAGGAGTT GGCCCAGGAA GTTCCTAGAC CAGTGGCTTC TCGCAGGAAG 
GGGCCAGGGT TTGCGTCATC GCTTGTCACC TTGCTGGCAT TCACACTAGG CTTTACGATG 
AGCCTGTGCT TTTGGCAGTC CTTCATGATC TTCAATGATT TGCTGCTCTG GGAACCAACA 
GCTCCTTCGC CAGTAGAGAC ATTTGAAAAT ATTTCGTATG GTATTGGGCT TTCAGTAGCC 
AGTCAGCTGA TTAATACCTT CAGCAAGGAT AGTTTTAGTC CGTTTAAATC TACAGTTCAC 
TATGATGAGG AAAGTGGATC TGAGGAGGAG ACTTTGCAGT ATTTTTCTAC TGTTGATCCC 
AATTATCGTT CTCGTAGGAA AAAAAAACTA GACTCCCAGA ACAAGAACTC ACAGAGTTTT 
CTCTCAGTTC TTCTGAGTAT TAATCATGGG TTAATTGCAG TGTTTACAGA TGTAAAGCAA 
GATAACGGAG ATCTGTTGGA AAACAAGCAT GGTGAATTCT GGTTAGAGTT CAGCAGCGGC 
TCGTTACTTT GTGTGACGAA GTATGAAGGC TTCGACGACA AGCACTACGT GTGCCTTCAC 
TGCGGCAGTT TCAGCCTCTA CCACAAAGGC ATAGTGGATG GAGCAGTTCC TTCTACAGAA 
ACACGACTGC CTAGTTCAAC CCGCCCACAT TGGTTAGAAC CTACTATTTA TTCCTCTGAA 
GAAGATGGCC TCAGTCGAGC TTCTTCAGAT GGTGTTGGAG GAGACACTTT GAATATGCTG 
TCTGTTGCAG TTAAAATAGT GTCTGATAAA TCAGAGTCCA ATACAAAGGA GTTTCTCATT 
GCTGTAGGGC TAAAAGGAGC TACTCTCCAG CATAGAATGC TTCCTTCTGG GCTTAGCTGG 
CATGAGCAGA TTTTATACTT CTTGAATATT GCTGATGAAC CTGTTCTGGG ATATAATCCT 
CCAACTTCAT TTACAACCTT TCATGTTCAT CTCTGGAGCT GTGCACTTGA TTATAGGCCT 
CTTTATTTGC CAATCCGATC TCTTCTTACT GTTGAAACAT TCAGCGTTTC AAGTAGTGTC 
GCCTTGGATA AATCATCTTC TACTCTCAGA ATAATTTTGG ATGAAGCTGC TTTACACCTG 
TCTGACAAGT GTAATACTGT TACTATAAAT TTGAATAGAG ATTATGTTCG TGTGATGGAT 
ATGGGACTTT TGGAATTAAC CATAACTGCA GTAAAGTCTG ATTCCGATGG AGAGCAAACT 
GAACCGCACT TTGAATTGCA CTGTTCGAGT GACGTCGTCC ATGTTAGAAC GTGCTCAGAC 
TCTTGTGCTG CTCTAATGAA TCTAATTCAA TATATTGCAA GCTATGGGGA CTTACATGCC 
ACTAACAAGG CAGACGTGAA GCCAGGAGCT CCTCAAAGAA AGGTAGATTC TGTTGGTCGG 
TCATCCTCAC GTGGTCCAGT ACTTCCTGAA GCAGATCAAC AGATATTACG AGATTTGATG 
AGTGATGCTA TGGAGGAGAT TGACATGCAA GAAGCAGCTT CATCTGTAAA ACCACAGTCT 
AATGGTGTTT CTGATGAAAA ATCTCAAGTT CAGGAACCAT GTTGTTCAGA CCTCTTCCTG 
TTTCCTGATG AGAGTGGAAA CGTGTCCCAG GAGCCTGGCC CCACTTACGC CTCATTCACC 
CACCACTTGA TTAGTGATGC AGTGACAGGC GTGCCCACGG AAAATGACGA CTTTTGCATT 
CTTTTTGCAC CAAGAGCAAC TGTTCAGGAG AAGGAAGAAG AACCAGTTGT AAAGATAATG 
GTTGATGATC CAATTGTGAT AAAAGAGAAT TATTTTAGTC TGCCTGTTAA GAAGACAGAT 
ACGAGCAAAG CCCCGTTACA CTTTCCCATT CCTGTCGTTC GGTACCTGGT TAAAGAAGTC 
TCTCTTGTTT GGCATCTTTA TGGAGGAAAA GATTTTGGAA CAGCCCCTCC TACTTCTCCA 
GCTAAAAGTT ACATTAGTCC CCATAGCTCA CCTTCTCACA CACCTACAAG ACATGGACAT 
AATATGGTTT GTGGGGGAAA AGGAAGAAAC CATGACTTTT TAATGGAAAT ACAGTTAAGC 
AAGGTGAAGT TTCAGCACGA GGTGTACCCG CCCTGCAAAC CAGAATGCGA GTCGAGCCTT 
GTGGAGCACC CGGTGTCCCG GCAGGTGTTC ATCGTGCAGG ATCTTGAAAT TCGAGATCGT 
CTGGCAACGT CACAAATGAA TAAATTTTTG TACCTGTATT GCAGTAAAGA AATGCCTCGG 
AAAGCTCATT CCAACATGTT GACAATTAAA GCACTACACG TGCGTCCAGA GTCTGGCAGG 
TCCCCACAGG AGTGCTGCCT CAGAGTATCA CTAATGCCAC TTCGCCTCAA TATTGACCAG 
GATGCCTTGT TCTTCTTGAA GGATTTCTTC ACAAGTCTTT CTACAGAAGT AGAGCTGCTC 
ATGACTCCAG ATCCAGAAGT TAAAAAGTCT CCAGGGGCTG ATGTCACCTG CAGTTTGCCA 
AGGCATTTAA GCGCCTCAAA AGAGCCAAAT CTAGTTCTTT CTTTCCCTGG GCCAAAGCAG 
CCTTCCCCAA ATGGCAGTGC CAATTCAGTG CAAGCGGTTA ATGGGGAAGA AGAGAAGGAC 
TTCTCTCCTG AAGAAGCGTT GTTTAGTGAT CAGCCTGTGT TTTTTAGAGA ATTTAGATTC 
ACATCAGAAG TTCCTATTCG ACTTGATTAT CATGGGAAAC ATGTATCAAT GGATCAGGGT 
ACATTAGCTG GGATTTTGAT TGGTCTCGCC CAGTTAAACT GCTCTGAACT AAAGCTGAAG 
AGGCTTTTCT ATCGGCATGG TTTGCTAGGT GTGGACAAAT TATTCTCATA CGCAATCACC 
GAGTGGCTTA CTGACATTAA GAAGAACCAG CTGCCAGGAA TCCTGGGAGG TGTTGGGCCT 
ATGCATTCAC TAGTACAGCT AGTGCAAGGC CTCAAGGACT TGGTCTGGTT ACCAATTGAG 
CAGTACCGAA AGGACGGCCG CATTGTCAGA GGGTTTCAGA GAGGGGCTGC CTCCTTTGGC 
ACCTCGACAG CAATGGCTGC TCTCGAGCTC ACGAACAGAA TGGTTCAAAC CATACAGGCA 
GCAGCGGAGA CTGCGTATGA CATGGTGTCT CCAGGCACCC TTTCTATCGA GCCCAAGAAG 
GTCAGGAGGT TCCCCCATCA CCGGTTAGCC CACCAGCCAG TGGACCTGAG GGAAGGGGTG 
GCCAAGGCCT ACAGTGTCGT GAAGGAGGGA ATCGCAGACA CGGCTCAGAC CATTTACGAA 
ACTGCTGCTC GGGAACACGA GAGCCGGGGG GTGACGGGCG CCGTGGGGGA AGTCCTGCGC 
CAGCTCCCCC CGGCCGTGGT GAAACCTCTG ATCGTCGCCA CAGAAGCGAC ATCGAATGTC 
TTGGGTGGCA TGAGAAACCA GATCAGACCA GATGTTCGGC AGGACGAGTC GCAGAAGTGG 
CGCCATGGGG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_010817249.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815551.1 Bos taurus autophagy related 2B (ATG2B), transcript variant X3, mRNA.

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CloneID	OBa50164
Clone ID Related Accession (Same CDS sequence)	XM_010817249.2
Accession Version	XM_010817249.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	autophagy-related protein 2 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTG CTTCTGAGCC CATGTATTGG TCAAGCTTCA TGACCAGCAG TATGCAATTG 
GCAAAAGAAT GTCTTAGCCA GAAACTAACA GATGAACAAG GAGAAGGATC TCAGCCTTTT 
GAAGGACTTG AAAAGTTTGC TGAAACCATC GAAACAGTAC TAAGAAGAGT AAAGGTCACT 
TTTATAGACA CTGTCTTGAG AATTGAACAC GTGCCAGAAA ATTCCAAGAC TGGAACTGCA 
CTTGAAATTC GAATAGAAAG AACCATGTAC TGTGATGAAA CTGCTGACGA GTCCTCAGGA 
ATTAACGTAC ATCAACCCAC AGCTTTTGCT CACAAGTTAC TTCAGCTCTC TGGAGTGTCT 
CTCTTCTGGG ATGAGTTTTC TGCATCTGCA AAATCCTCCC CAGTGTGTTC AACTGTGCCA 
GTGGAAACAG AGCCAAAGCT GTCACCTAGC TGGAATCCAA AAATTGTTTA TGAACCTCAC 
CCACAACTAA CAAGGAACTT GCCAGAAGTA GCACCTTCTG ACCCAGTGCA GATAGGAGGG 
CTCAATGGTA GGCTGGAGTT GAGTCTCACA TTGAAACAGA ATGAAGTACT TCCTGGAGCT 
AAGCTGGATA TTGATGGGCA GATAGACTCT ATACATCTGT TCCTGTCACC CAGGCAGGTG 
CACTTACTTT TGGACATGTT AGCAGCTATT GCTGGGCCAG AAAATTCAAG CAAAATTGGA 
TTAGCTAATA AAGATAGAAA AAACCGACCC ATGCAGCAAG AAGATGAATA TCGAATTCAA 
ATGGAATTGA ACCGGTATTA TTTGAGGAAA GACTCCCTCT CTGCCGGTGT GTCCTCAGAG 
CAAAGCTTTT ATGAGACAGA AACGGCTCGT ACACCTTCTA GCCGGGAAGA AGAAGTCTTC 
TTTTCCATGG CTGATATGGA CATGTCGCAC AGTCTCTCTT CTCTTCCACC CCTTGGGGAA 
CCCCCAAATA TGGACCTTGA GTTATCATTA ACTAGTACAT ATACAAACAC CCCAGCAGGA 
TCTCCATTAA GTACTACTGT GCTTCAGCCA ACATGGGGAG ACTTCCTTGA TCATCATAAG 
GAACAGCCAG TCAGAGGATC AGCGTTACCC TCCAACCTGG TTCACCCAGC ATCTTTACAA 
AAGACTGCTC TCCCCTCTAG GTCTGTTTCA GTGGATGAAT CCAGGCCTGA ACTTATTTTT 
AGAGTAGCTG TGGGAACGTT TTCAATCTCT GTGCTTCACA TTGATCCTTT ATCTCCACCT 
GAATCATCAC TGAATGTTAA TCCATTGACA CCAATGGCGA TAGCTTTCTT TACTTGTATA 
GAAAAGATTG ATCCAGCACA ATTTTCAACA GAAGATTTTA AGTCTTTCCG AGCAGTATTT 
GCAGAAGCTT GCTCACATGA TCACCTTAGA TTTATAGGCA CTGGCATCAA AGTGTCCTAT 
GAACAAAGAC AAAGATCGGC GTCCCGATAT TTCAGTACTG ATATGTCCAT TGGGCAAATG 
GAACTTTTGG AATGCCTGTT TCCAACTGAT TCTCATTCTG TTCCTCCTCA CTATACAGAG 
CTTTTGATGT TCCATTCCAA AGAACGAACT GATTCTCACC TCCCTGTGTG TCTTCAGCTT 
CATTATAAGC ATTCTGAGAA TAAAGGACCT CAGGCTGGTC AAGCAAGACT TAGTTCCATT 
CCTCAGAAGG CAGAATTACA GGTTAAGTTA AATCCAGTGT TTTGTGAGCT GGATATCAGT 
ATTGTGGACA GGTTAAATTC CTTGCTTCAA CCACAGAAAC TCACTACAGT GGAGATGATG 
GCATCGCATA TGTATACTTC ATATAATAAG CATATTAGTT TGCACAAAGC TTTCACTGAA 
GTATTTCTAG ATGATTCACA TAATCCTGCA AACTGCCGGG TATCAGTACA AGTTGCCACG 
CCAGCATTAA ACCTTTCTGT TCGCTTCCCA ATACCTGATC TTCGGTCAGA TCAAGAGAGA 
GGACCATGGT TTAAGAAGTC ACTTCAGAAG GAGATTCTTC ATTTAGCATT CACAGATCTA 
GAGTTTAAAA CTGAATTCCT AGGAGGATCA ACCCCTGAAC AAGTTAAATT GGAACTTACA 
TTTAGAGAAC TAGTCGGGTC ATTCCAGGAA GACAAAGGAG AACCATCTAT TAAGTTTTTC 
CATGTGGCTA GTGGAGGAGA TGGAGATACA ACGTCATCAG ATGACTTTGA CTGGCCGCGA 
ATTGTACTAA AAGTGAACCC ACCAGCCATG CATTCCATTT TGGAGAGAAT AGCAGCTGAG 
GAGGAAGAGG AAAGTGACGG CCACTATCAG GAGGAAGAGG AGGGAGGCGC TCATTCCCTG 
AAGGATGTCT GTGATCTGAG AAGGCCAGCT CCGTCTCCCT TCTCTTCCCG TAGAGTAATG 
TTTGAAAATG AACAGATGGT GATGCCAGGA GACCCTGTAG AAATGACAGA GTTTCAGAAT 
AAAGCAATCA GCAATTCTCA CTATGTGCTG GAACTTACAT TACCCAATAT TCATATAACA 
CTACCTAATA AAAGCTTTTA TGAGAAGCTT TATAATAGGG CCTCAGAAGC TCAGGAGTTG 
GCCCAGGAAG TTCCTAGACC AGTGGCTTCT CGCAGGAAGG GGCCAGGGTT TGCGTCATCG 
CTTGTCACCT TGCTGGCATT CACACTAGGC TTTACGATGA GCCTGTGCTT TTGGCAGTCC 
TTCATGATCT TCAATGATTT GCTGCTCTGG GAACCAACAG CTCCTTCGCC AGTAGAGACA 
TTTGAAAATA TTTCGTATGG TATTGGGCTT TCAGTAGCCA GTCAGCTGAT TAATACCTTC 
AGCAAGGATA GTTTTAGTCC GTTTAAATCT ACAGTTCACT ATGATGAGGA AAGTGGATCT 
GAGGAGGAGA CTTTGCAGTA TTTTTCTACT GTTGATCCCA ATTATCGTTC TCGTAGGAAA 
AAAAAACTAG ACTCCCAGAA CAAGAACTCA CAGAGTTTTC TCTCAGTTCT TCTGAGTATT 
AATCATGGGT TAATTGCAGT GTTTACAGAT GTAAAGCAAG ATAACGGAGA TCTGTTGGAA 
AACAAGCATG GTGAATTCTG GTTAGAGTTC AGCAGCGGCT CGTTACTTTG TGTGACGAAG 
TATGAAGGCT TCGACGACAA GCACTACGTG TGCCTTCACT GCGGCAGTTT CAGCCTCTAC 
CACAAAGGCA TAGTGGATGG AGCAGTTCCT TCTACAGAAA CACGACTGCC TAGTTCAACC 
CGCCCACATT GGTTAGAACC TACTATTTAT TCCTCTGAAG AAGATGGCCT CAGTCGAGCT 
TCTTCAGATG GTGTTGGAGG AGACACTTTG AATATGCTGT CTGTTGCAGT TAAAATAGTG 
TCTGATAAAT CAGAGTCCAA TACAAAGGAG TTTCTCATTG CTGTAGGGCT AAAAGGAGCT 
ACTCTCCAGC ATAGAATGCT TCCTTCTGGG CTTAGCTGGC ATGAGCAGAT TTTATACTTC 
TTGAATATTG CTGATGAACC TGTTCTGGGA TATAATCCTC CAACTTCATT TACAACCTTT 
CATGTTCATC TCTGGAGCTG TGCACTTGAT TATAGGCCTC TTTATTTGCC AATCCGATCT 
CTTCTTACTG TTGAAACATT CAGCGTTTCA AGTAGTGTCG CCTTGGATAA ATCATCTTCT 
ACTCTCAGAA TAATTTTGGA TGAAGCTGCT TTACACCTGT CTGACAAGTG TAATACTGTT 
ACTATAAATT TGAATAGAGA TTATGTTCGT GTGATGGATA TGGGACTTTT GGAATTAACC 
ATAACTGCAG TAAAGTCTGA TTCCGATGGA GAGCAAACTG AACCGCACTT TGAATTGCAC 
TGTTCGAGTG ACGTCGTCCA TGTTAGAACG TGCTCAGACT CTTGTGCTGC TCTAATGAAT 
CTAATTCAAT ATATTGCAAG CTATGGGGAC TTACATGCCA CTAACAAGGC AGACGTGAAG 
CCAGGAGCTC CTCAAAGAAA GGTGAAGGTA GATTCTGTTG GTCGGTCATC CTCACGTGGT 
CCAGTACTTC CTGAAGCAGA TCAACAGATA TTACGAGATT TGATGAGTGA TGCTATGGAG 
GAGATTGACA TGCAAGAAGC AGCTTCATCT GTAAAACCAC AGTCTAATGG TGTTTCTGAT 
GAAAAATCTC AAGTTCAGGA ACCATGTTGT TCAGACCTCT TCCTGTTTCC TGATGAGAGT 
GGAAACGTGT CCCAGGAGCC TGGCCCCACT TACGCCTCAT TCACCCACCA CTTGATTAGT 
GATGCAGTGA CAGGCGTGCC CACGGAAAAT GACGACTTTT GCATTCTTTT TGCACCAAGA 
GCAACTGTTC AGGAGAAGGA AGAAGAACCA GTTGTAAAGA TAATGGTTGA TGATCCAATT 
GTGATAAAAG AGAATTATTT TAGTCTGCCT GTTAAGAAGA CAGATACGAG CAAAGCCCCG 
TTACACTTTC CCATTCCTGT CGTTCGGTAC CTGGTTAAAG AAGTCTCTCT TGTTTGGCAT 
CTTTATGGAG GAAAAGATTT TGGAACAGCC CCTCCTACTT CTCCAGCTAA AAGTTACATT 
AGTCCCCATA GCTCACCTTC TCACACACCT ACAAGACATG GACATAATAT GGTTTGTGGG 
GGAAAAGGAA GAAACCATGA CTTTTTAATG GAAATACAGT TAAGCAAGGT GAAGTTTCAG 
CACGAGGTGT ACCCGCCCTG CAAACCAGAA TGCGAGTCGA GCCTTGTGGA GCACCCGGTG 
TCCCGGCAGG TGTTCATCGT GCAGGATCTT GAAATTCGAG ATCGTCTGGC AACGTCACAA 
ATGAATAAAT TTTTGTACCT GTATTGCAGT AAAGAAATGC CTCGGAAAGC TCATTCCAAC 
ATGTTGACAA TTAAAGCACT ACACGTGCGT CCAGAGTCTG GCAGGTCCCC ACAGGAGTGC 
TGCCTCAGAG TATCACTAAT GCCACTTCGC CTCAATATTG ACCAGGATGC CTTGTTCTTC 
TTGAAGGATT TCTTCACAAG TCTTTCTACA GAAGTAGAGC TGCTCATGAC TCCAGATCCA 
GAAGTTAAAA AGTCTCCAGG GGCTGATGTC ACCTGCAGTT TGCCAAGGCA TTTAAGCGCC 
TCAAAAGAGC CAAATCTAGT TCTTTCTTTC CCTGGGCCAA AGCAGCCTTC CCCAAATGGC 
AGTGCCAATT CAGTGCAAGC GGTTAATGGG GAAGAAGAGA AGGACTTCTC TCCTGAAGAA 
GCGTTGTTTA GTGATCAGCC TGTGTTTTTT AGAGAATTTA GATTCACATC AGAAGTTCCT 
ATTCGACTTG ATTATCATGG GAAACATGTA TCAATGGATC AGGGTACATT AGCTGGGATT 
TTGATTGGTC TCGCCCAGTT AAACTGCTCT GAACTAAAGC TGAAGAGGCT TTTCTATCGG 
CATGGTTTGC TAGGTGTGGA CAAATTATTC TCATACGCAA TCACCGAGTG GCTTACTGAC 
ATTAAGAAGA ACCAGCTGCC AGGAATCCTG GGAGGTGTTG GGCCTATGCA TTCACTAGTA 
CAGCTAGTGC AAGGCCTCAA GGACTTGGTC TGGTTACCAA TTGAGCAGTA CCGAAAGGAC 
GGCCGCATTG TCAGAGGGTT TCAGAGAGGG GCTGCCTCCT TTGGCACCTC GACAGCAATG 
GCTGCTCTCG AGCTCACGAA CAGAATGGTT CAAACCATAC AGGCAGCAGC GGAGACTGCG 
TATGACATGG TGTCTCCAGG CACCCTTTCT ATCGAGCCCA AGAAGGTCAG GAGGTTCCCC 
CATCACCGGT TAGCCCACCA GCCAGTGGAC CTGAGGGAAG GGGTGGCCAA GGCCTACAGT 
GTCGTGAAGG AGGGAATCGC AGACACGGCT CAGACCATTT ACGAAACTGC TGCTCGGGAA 
CACGAGAGCC GGGGGGTGAC GGGCGCCGTG GGGGAAGTCC TGCGCCAGCT CCCCCCGGCC 
GTGGTGAAAC CTCTGATCGT CGCCACAGAA GCGACATCGA ATGTCTTGGG TGGCATGAGA 
AACCAGATCA GACCAGATGT TCGGCAGGAC GAGTCGCAGA AGTGGCGCCA TGGGGACGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815551.1
CDS	97..6159
Translation	MASASEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTVPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSISVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQAGQARLSSIPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRASEAQELAQEVPRPVASRRKGPGFASSLVTLLAFTLGFTMSLCFWQSFMIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWLEFSSGSLLCVTKYEGFDDKHYVCLHCGSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDPIVIKENYFSLPVKKTDTSKAPLHFPIPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMTPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_010817249.2
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy related 2B (ATG2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817249.2

ORF Insert Sequence:

ATGGCATCTG CTTCTGAGCC CATGTATTGG TCAAGCTTCA TGACCAGCAG TATGCAATTG 
GCAAAAGAAT GTCTTAGCCA GAAACTAACA GATGAACAAG GAGAAGGATC TCAGCCTTTT 
GAAGGACTTG AAAAGTTTGC TGAAACCATC GAAACAGTAC TAAGAAGAGT AAAGGTCACT 
TTTATAGACA CTGTCTTGAG AATTGAACAC GTGCCAGAAA ATTCCAAGAC TGGAACTGCA 
CTTGAAATTC GAATAGAAAG AACCATGTAC TGTGATGAAA CTGCTGACGA GTCCTCAGGA 
ATTAACGTAC ATCAACCCAC AGCTTTTGCT CACAAGTTAC TTCAGCTCTC TGGAGTGTCT 
CTCTTCTGGG ATGAGTTTTC TGCATCTGCA AAATCCTCCC CAGTGTGTTC AACTGTGCCA 
GTGGAAACAG AGCCAAAGCT GTCACCTAGC TGGAATCCAA AAATTGTTTA TGAACCTCAC 
CCACAACTAA CAAGGAACTT GCCAGAAGTA GCACCTTCTG ACCCAGTGCA GATAGGAGGG 
CTCAATGGTA GGCTGGAGTT GAGTCTCACA TTGAAACAGA ATGAAGTACT TCCTGGAGCT 
AAGCTGGATA TTGATGGGCA GATAGACTCT ATACATCTGT TCCTGTCACC CAGGCAGGTG 
CACTTACTTT TGGACATGTT AGCAGCTATT GCTGGGCCAG AAAATTCAAG CAAAATTGGA 
TTAGCTAATA AAGATAGAAA AAACCGACCC ATGCAGCAAG AAGATGAATA TCGAATTCAA 
ATGGAATTGA ACCGGTATTA TTTGAGGAAA GACTCCCTCT CTGCCGGTGT GTCCTCAGAG 
CAAAGCTTTT ATGAGACAGA AACGGCTCGT ACACCTTCTA GCCGGGAAGA AGAAGTCTTC 
TTTTCCATGG CTGATATGGA CATGTCGCAC AGTCTCTCTT CTCTTCCACC CCTTGGGGAA 
CCCCCAAATA TGGACCTTGA GTTATCATTA ACTAGTACAT ATACAAACAC CCCAGCAGGA 
TCTCCATTAA GTACTACTGT GCTTCAGCCA ACATGGGGAG ACTTCCTTGA TCATCATAAG 
GAACAGCCAG TCAGAGGATC AGCGTTACCC TCCAACCTGG TTCACCCAGC ATCTTTACAA 
AAGACTGCTC TCCCCTCTAG GTCTGTTTCA GTGGATGAAT CCAGGCCTGA ACTTATTTTT 
AGAGTAGCTG TGGGAACGTT TTCAATCTCT GTGCTTCACA TTGATCCTTT ATCTCCACCT 
GAATCATCAC TGAATGTTAA TCCATTGACA CCAATGGCGA TAGCTTTCTT TACTTGTATA 
GAAAAGATTG ATCCAGCACA ATTTTCAACA GAAGATTTTA AGTCTTTCCG AGCAGTATTT 
GCAGAAGCTT GCTCACATGA TCACCTTAGA TTTATAGGCA CTGGCATCAA AGTGTCCTAT 
GAACAAAGAC AAAGATCGGC GTCCCGATAT TTCAGTACTG ATATGTCCAT TGGGCAAATG 
GAACTTTTGG AATGCCTGTT TCCAACTGAT TCTCATTCTG TTCCTCCTCA CTATACAGAG 
CTTTTGATGT TCCATTCCAA AGAACGAACT GATTCTCACC TCCCTGTGTG TCTTCAGCTT 
CATTATAAGC ATTCTGAGAA TAAAGGACCT CAGGCTGGTC AAGCAAGACT TAGTTCCATT 
CCTCAGAAGG CAGAATTACA GGTTAAGTTA AATCCAGTGT TTTGTGAGCT GGATATCAGT 
ATTGTGGACA GGTTAAATTC CTTGCTTCAA CCACAGAAAC TCACTACAGT GGAGATGATG 
GCATCGCATA TGTATACTTC ATATAATAAG CATATTAGTT TGCACAAAGC TTTCACTGAA 
GTATTTCTAG ATGATTCACA TAATCCTGCA AACTGCCGGG TATCAGTACA AGTTGCCACG 
CCAGCATTAA ACCTTTCTGT TCGCTTCCCA ATACCTGATC TTCGGTCAGA TCAAGAGAGA 
GGACCATGGT TTAAGAAGTC ACTTCAGAAG GAGATTCTTC ATTTAGCATT CACAGATCTA 
GAGTTTAAAA CTGAATTCCT AGGAGGATCA ACCCCTGAAC AAGTTAAATT GGAACTTACA 
TTTAGAGAAC TAGTCGGGTC ATTCCAGGAA GACAAAGGAG AACCATCTAT TAAGTTTTTC 
CATGTGGCTA GTGGAGGAGA TGGAGATACA ACGTCATCAG ATGACTTTGA CTGGCCGCGA 
ATTGTACTAA AAGTGAACCC ACCAGCCATG CATTCCATTT TGGAGAGAAT AGCAGCTGAG 
GAGGAAGAGG AAAGTGACGG CCACTATCAG GAGGAAGAGG AGGGAGGCGC TCATTCCCTG 
AAGGATGTCT GTGATCTGAG AAGGCCAGCT CCGTCTCCCT TCTCTTCCCG TAGAGTAATG 
TTTGAAAATG AACAGATGGT GATGCCAGGA GACCCTGTAG AAATGACAGA GTTTCAGAAT 
AAAGCAATCA GCAATTCTCA CTATGTGCTG GAACTTACAT TACCCAATAT TCATATAACA 
CTACCTAATA AAAGCTTTTA TGAGAAGCTT TATAATAGGG CCTCAGAAGC TCAGGAGTTG 
GCCCAGGAAG TTCCTAGACC AGTGGCTTCT CGCAGGAAGG GGCCAGGGTT TGCGTCATCG 
CTTGTCACCT TGCTGGCATT CACACTAGGC TTTACGATGA GCCTGTGCTT TTGGCAGTCC 
TTCATGATCT TCAATGATTT GCTGCTCTGG GAACCAACAG CTCCTTCGCC AGTAGAGACA 
TTTGAAAATA TTTCGTATGG TATTGGGCTT TCAGTAGCCA GTCAGCTGAT TAATACCTTC 
AGCAAGGATA GTTTTAGTCC GTTTAAATCT ACAGTTCACT ATGATGAGGA AAGTGGATCT 
GAGGAGGAGA CTTTGCAGTA TTTTTCTACT GTTGATCCCA ATTATCGTTC TCGTAGGAAA 
AAAAAACTAG ACTCCCAGAA CAAGAACTCA CAGAGTTTTC TCTCAGTTCT TCTGAGTATT 
AATCATGGGT TAATTGCAGT GTTTACAGAT GTAAAGCAAG ATAACGGAGA TCTGTTGGAA 
AACAAGCATG GTGAATTCTG GTTAGAGTTC AGCAGCGGCT CGTTACTTTG TGTGACGAAG 
TATGAAGGCT TCGACGACAA GCACTACGTG TGCCTTCACT GCGGCAGTTT CAGCCTCTAC 
CACAAAGGCA TAGTGGATGG AGCAGTTCCT TCTACAGAAA CACGACTGCC TAGTTCAACC 
CGCCCACATT GGTTAGAACC TACTATTTAT TCCTCTGAAG AAGATGGCCT CAGTCGAGCT 
TCTTCAGATG GTGTTGGAGG AGACACTTTG AATATGCTGT CTGTTGCAGT TAAAATAGTG 
TCTGATAAAT CAGAGTCCAA TACAAAGGAG TTTCTCATTG CTGTAGGGCT AAAAGGAGCT 
ACTCTCCAGC ATAGAATGCT TCCTTCTGGG CTTAGCTGGC ATGAGCAGAT TTTATACTTC 
TTGAATATTG CTGATGAACC TGTTCTGGGA TATAATCCTC CAACTTCATT TACAACCTTT 
CATGTTCATC TCTGGAGCTG TGCACTTGAT TATAGGCCTC TTTATTTGCC AATCCGATCT 
CTTCTTACTG TTGAAACATT CAGCGTTTCA AGTAGTGTCG CCTTGGATAA ATCATCTTCT 
ACTCTCAGAA TAATTTTGGA TGAAGCTGCT TTACACCTGT CTGACAAGTG TAATACTGTT 
ACTATAAATT TGAATAGAGA TTATGTTCGT GTGATGGATA TGGGACTTTT GGAATTAACC 
ATAACTGCAG TAAAGTCTGA TTCCGATGGA GAGCAAACTG AACCGCACTT TGAATTGCAC 
TGTTCGAGTG ACGTCGTCCA TGTTAGAACG TGCTCAGACT CTTGTGCTGC TCTAATGAAT 
CTAATTCAAT ATATTGCAAG CTATGGGGAC TTACATGCCA CTAACAAGGC AGACGTGAAG 
CCAGGAGCTC CTCAAAGAAA GGTGAAGGTA GATTCTGTTG GTCGGTCATC CTCACGTGGT 
CCAGTACTTC CTGAAGCAGA TCAACAGATA TTACGAGATT TGATGAGTGA TGCTATGGAG 
GAGATTGACA TGCAAGAAGC AGCTTCATCT GTAAAACCAC AGTCTAATGG TGTTTCTGAT 
GAAAAATCTC AAGTTCAGGA ACCATGTTGT TCAGACCTCT TCCTGTTTCC TGATGAGAGT 
GGAAACGTGT CCCAGGAGCC TGGCCCCACT TACGCCTCAT TCACCCACCA CTTGATTAGT 
GATGCAGTGA CAGGCGTGCC CACGGAAAAT GACGACTTTT GCATTCTTTT TGCACCAAGA 
GCAACTGTTC AGGAGAAGGA AGAAGAACCA GTTGTAAAGA TAATGGTTGA TGATCCAATT 
GTGATAAAAG AGAATTATTT TAGTCTGCCT GTTAAGAAGA CAGATACGAG CAAAGCCCCG 
TTACACTTTC CCATTCCTGT CGTTCGGTAC CTGGTTAAAG AAGTCTCTCT TGTTTGGCAT 
CTTTATGGAG GAAAAGATTT TGGAACAGCC CCTCCTACTT CTCCAGCTAA AAGTTACATT 
AGTCCCCATA GCTCACCTTC TCACACACCT ACAAGACATG GACATAATAT GGTTTGTGGG 
GGAAAAGGAA GAAACCATGA CTTTTTAATG GAAATACAGT TAAGCAAGGT GAAGTTTCAG 
CACGAGGTGT ACCCGCCCTG CAAACCAGAA TGCGAGTCGA GCCTTGTGGA GCACCCGGTG 
TCCCGGCAGG TGTTCATCGT GCAGGATCTT GAAATTCGAG ATCGTCTGGC AACGTCACAA 
ATGAATAAAT TTTTGTACCT GTATTGCAGT AAAGAAATGC CTCGGAAAGC TCATTCCAAC 
ATGTTGACAA TTAAAGCACT ACACGTGCGT CCAGAGTCTG GCAGGTCCCC ACAGGAGTGC 
TGCCTCAGAG TATCACTAAT GCCACTTCGC CTCAATATTG ACCAGGATGC CTTGTTCTTC 
TTGAAGGATT TCTTCACAAG TCTTTCTACA GAAGTAGAGC TGCTCATGAC TCCAGATCCA 
GAAGTTAAAA AGTCTCCAGG GGCTGATGTC ACCTGCAGTT TGCCAAGGCA TTTAAGCGCC 
TCAAAAGAGC CAAATCTAGT TCTTTCTTTC CCTGGGCCAA AGCAGCCTTC CCCAAATGGC 
AGTGCCAATT CAGTGCAAGC GGTTAATGGG GAAGAAGAGA AGGACTTCTC TCCTGAAGAA 
GCGTTGTTTA GTGATCAGCC TGTGTTTTTT AGAGAATTTA GATTCACATC AGAAGTTCCT 
ATTCGACTTG ATTATCATGG GAAACATGTA TCAATGGATC AGGGTACATT AGCTGGGATT 
TTGATTGGTC TCGCCCAGTT AAACTGCTCT GAACTAAAGC TGAAGAGGCT TTTCTATCGG 
CATGGTTTGC TAGGTGTGGA CAAATTATTC TCATACGCAA TCACCGAGTG GCTTACTGAC 
ATTAAGAAGA ACCAGCTGCC AGGAATCCTG GGAGGTGTTG GGCCTATGCA TTCACTAGTA 
CAGCTAGTGC AAGGCCTCAA GGACTTGGTC TGGTTACCAA TTGAGCAGTA CCGAAAGGAC 
GGCCGCATTG TCAGAGGGTT TCAGAGAGGG GCTGCCTCCT TTGGCACCTC GACAGCAATG 
GCTGCTCTCG AGCTCACGAA CAGAATGGTT CAAACCATAC AGGCAGCAGC GGAGACTGCG 
TATGACATGG TGTCTCCAGG CACCCTTTCT ATCGAGCCCA AGAAGGTCAG GAGGTTCCCC 
CATCACCGGT TAGCCCACCA GCCAGTGGAC CTGAGGGAAG GGGTGGCCAA GGCCTACAGT 
GTCGTGAAGG AGGGAATCGC AGACACGGCT CAGACCATTT ACGAAACTGC TGCTCGGGAA 
CACGAGAGCC GGGGGGTGAC GGGCGCCGTG GGGGAAGTCC TGCGCCAGCT CCCCCCGGCC 
GTGGTGAAAC CTCTGATCGT CGCCACAGAA GCGACATCGA ATGTCTTGGG TGGCATGAGA 
AACCAGATCA GACCAGATGT TCGGCAGGAC GAGTCGCAGA AGTGGCGCCA TGGGGACGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ATG2B
Entrez Gene ID	519961
Full Name	autophagy related 2B
General protein information	Preferred Names autophagy related 2B Names autophagy-related protein 2 homolog B ATG2 autophagy related 2 homolog B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Mus musculus (house mouse)	Atg2b	NP_083930.5
Canis lupus familiaris (dog)	ATG2B	XP_537549.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004092	XP_312964.5
Gallus gallus (chicken)	ATG2B	XP_421350.3
Drosophila melanogaster (fruit fly)	Atg2	NP_001261341.1
Xenopus tropicalis (tropical clawed frog)	atg2b	NP_001072626.1
Homo sapiens (human)	ATG2B	NP_060506.5
Bos taurus (cattle)	ATG2B	NP_001193682.1
Pan troglodytes (chimpanzee)	ATG2B	XP_510152.3
Macaca mulatta (Rhesus monkey)	ATG2B	XP_001101467.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ATG2B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

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