TSC1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSC1
Entrez Gene ID	102991442
Full Name	TSC complex subunit 1
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007101478.1	XP_007101540.1	hamartin isoform X3
XM_007101477.1	XP_007101539.1	hamartin
XM_028497223.1	XP_028353024.1	hamartin isoform X1
XM_007101478.2	XP_007101540.1	hamartin
XM_024117344.1	XP_023973112.1	hamartin
XM_007101476.2	XP_007101538.1	hamartin isoform X2
XM_007101476.1	XP_007101538.1	hamartin

Pathways from BioSystems
Gene Ontology

The following TSC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh111767	XM_007101476.2 Latest version!	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPh111767	XM_007101477.1 Latest version!	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPh111767	XM_024117344.1 Latest version!	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPh111767	XM_007101478.2 Latest version!	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPh320223	XM_028497223.1 Latest version!	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OPh111767	XM_007101478.1	Physeter catodon tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPh111767	XM_007101476.1	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSC1 ( XM_007101478.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007101540.1 Physeter catodon tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

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CloneID	OPh111767
Clone ID Related Accession (Same CDS sequence)	XM_007101478.1 , XM_007101477.1 , XM_007101478.2 , XM_024117344.1 , XM_007101476.2 , XM_007101476.1
Accession Version	XM_007101478.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	hamartin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006712863.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007101540.1
CDS	299..3796
Translation	MAQQANIGELLSMLDSPMLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSTLSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTASSYVDTQNSYGSATSTPHSTSRLMLLNTPGQLPQTLSSLSTRLLTEPPQATLWSPSVVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGRKGTPLGTPATSPPPAPPCHSDDYVHISLPQAMATAPKKEERADSARPCLHRQHHLPNDRGLEELPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLAPRGGFDSPFYRDSLPGCPRKTHSAVSSAQGTGANPEPLNSALDRLGPDTPKQAFTPIDLPCGGAEESPAGDRERQASLESSILTPSPFKIPPPRGVGFGGGQPPPYDHLFEVALPKTARHFVSKKTEGLLKKAKGNTEEDCTPSASPMEVLDRLIQQGADAHSKELNRLSLPSKSVDWTHFGGSAPSDETRSLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKTAYRKELEKNRSHVLQQNQRLDTAQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKTEAAEAAEERLDCCRDGCLDSVTGHNNEEASGPNGEAKPPRPGGARGGGGSRGGGGSSGSGSELSTPEKPPNPRAAPLGSRWETAVGEPATGIPVTVGSLPSSKSFLGMKARELFRNKSESQCDEDSLTSSSLSETLKTELGKDSGVEAKPPLNLDGPQPPPPKPDSVGQLRIMDYNEVQHEHS

Target ORF information:

RefSeq Version	XM_007101478.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007101478.1

ORF Insert Sequence:

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_007101477.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007101539.1 Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPh111767
Clone ID Related Accession (Same CDS sequence)	XM_007101478.1 , XM_007101477.1 , XM_007101478.2 , XM_024117344.1 , XM_007101476.2 , XM_007101476.1
Accession Version	XM_007101477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	hamartin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873644.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007101539.1
CDS	508..4005
Translation	MAQQANIGELLSMLDSPMLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSTLSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTASSYVDTQNSYGSATSTPHSTSRLMLLNTPGQLPQTLSSLSTRLLTEPPQATLWSPSVVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGRKGTPLGTPATSPPPAPPCHSDDYVHISLPQAMATAPKKEERADSARPCLHRQHHLPNDRGLEELPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLAPRGGFDSPFYRDSLPGCPRKTHSAVSSAQGTGANPEPLNSALDRLGPDTPKQAFTPIDLPCGGAEESPAGDRERQASLESSILTPSPFKIPPPRGVGFGGGQPPPYDHLFEVALPKTARHFVSKKTEGLLKKAKGNTEEDCTPSASPMEVLDRLIQQGADAHSKELNRLSLPSKSVDWTHFGGSAPSDETRSLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKTAYRKELEKNRSHVLQQNQRLDTAQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKTEAAEAAEERLDCCRDGCLDSVTGHNNEEASGPNGEAKPPRPGGARGGGGSRGGGGSSGSGSELSTPEKPPNPRAAPLGSRWETAVGEPATGIPVTVGSLPSSKSFLGMKARELFRNKSESQCDEDSLTSSSLSETLKTELGKDSGVEAKPPLNLDGPQPPPPKPDSVGQLRIMDYNEVQHEHS

Target ORF information:

RefSeq Version	XM_007101477.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007101477.1

ORF Insert Sequence:

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_007101478.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007101540.1 Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X4, mRNA.

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CloneID	OPh111767
Clone ID Related Accession (Same CDS sequence)	XM_007101478.1 , XM_007101477.1 , XM_007101478.2 , XM_024117344.1 , XM_007101476.2 , XM_007101476.1
Accession Version	XM_007101478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	hamartin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873644.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 23, 2018 this sequence version replaced XM_007101478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007101540.1
CDS	335..3832
Translation	MAQQANIGELLSMLDSPMLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSTLSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTASSYVDTQNSYGSATSTPHSTSRLMLLNTPGQLPQTLSSLSTRLLTEPPQATLWSPSVVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGRKGTPLGTPATSPPPAPPCHSDDYVHISLPQAMATAPKKEERADSARPCLHRQHHLPNDRGLEELPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLAPRGGFDSPFYRDSLPGCPRKTHSAVSSAQGTGANPEPLNSALDRLGPDTPKQAFTPIDLPCGGAEESPAGDRERQASLESSILTPSPFKIPPPRGVGFGGGQPPPYDHLFEVALPKTARHFVSKKTEGLLKKAKGNTEEDCTPSASPMEVLDRLIQQGADAHSKELNRLSLPSKSVDWTHFGGSAPSDETRSLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKTAYRKELEKNRSHVLQQNQRLDTAQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKTEAAEAAEERLDCCRDGCLDSVTGHNNEEASGPNGEAKPPRPGGARGGGGSRGGGGSSGSGSELSTPEKPPNPRAAPLGSRWETAVGEPATGIPVTVGSLPSSKSFLGMKARELFRNKSESQCDEDSLTSSSLSETLKTELGKDSGVEAKPPLNLDGPQPPPPKPDSVGQLRIMDYNEVQHEHS

Target ORF information:

RefSeq Version	XM_007101478.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007101478.2

ORF Insert Sequence:

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_024117344.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023973112.1 Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X3, mRNA.

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CloneID	OPh111767
Clone ID Related Accession (Same CDS sequence)	XM_007101478.1 , XM_007101477.1 , XM_007101478.2 , XM_024117344.1 , XM_007101476.2 , XM_007101476.1
Accession Version	XM_024117344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	hamartin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873644.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023973112.1
CDS	343..3840
Translation	MAQQANIGELLSMLDSPMLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSTLSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTASSYVDTQNSYGSATSTPHSTSRLMLLNTPGQLPQTLSSLSTRLLTEPPQATLWSPSVVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGRKGTPLGTPATSPPPAPPCHSDDYVHISLPQAMATAPKKEERADSARPCLHRQHHLPNDRGLEELPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLAPRGGFDSPFYRDSLPGCPRKTHSAVSSAQGTGANPEPLNSALDRLGPDTPKQAFTPIDLPCGGAEESPAGDRERQASLESSILTPSPFKIPPPRGVGFGGGQPPPYDHLFEVALPKTARHFVSKKTEGLLKKAKGNTEEDCTPSASPMEVLDRLIQQGADAHSKELNRLSLPSKSVDWTHFGGSAPSDETRSLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKTAYRKELEKNRSHVLQQNQRLDTAQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKTEAAEAAEERLDCCRDGCLDSVTGHNNEEASGPNGEAKPPRPGGARGGGGSRGGGGSSGSGSELSTPEKPPNPRAAPLGSRWETAVGEPATGIPVTVGSLPSSKSFLGMKARELFRNKSESQCDEDSLTSSSLSETLKTELGKDSGVEAKPPLNLDGPQPPPPKPDSVGQLRIMDYNEVQHEHS

Target ORF information:

RefSeq Version	XM_024117344.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024117344.1

ORF Insert Sequence:

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_007101476.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007101538.1 Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.

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CloneID	OPh111767
Clone ID Related Accession (Same CDS sequence)	XM_007101478.1 , XM_007101477.1 , XM_007101478.2 , XM_024117344.1 , XM_007101476.2 , XM_007101476.1
Accession Version	XM_007101476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	hamartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041226.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2019 this sequence version replaced XM_007101476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007101538.1
CDS	348..3845
Translation	MAQQANIGELLSMLDSPMLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSTLSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTASSYVDTQNSYGSATSTPHSTSRLMLLNTPGQLPQTLSSLSTRLLTEPPQATLWSPSVVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGRKGTPLGTPATSPPPAPPCHSDDYVHISLPQAMATAPKKEERADSARPCLHRQHHLPNDRGLEELPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLAPRGGFDSPFYRDSLPGCPRKTHSAVSSAQGTGANPEPLNSALDRLGPDTPKQAFTPIDLPCGGAEESPAGDRERQASLESSILTPSPFKIPPPRGVGFGGGQPPPYDHLFEVALPKTARHFVSKKTEGLLKKAKGNTEEDCTPSASPMEVLDRLIQQGADAHSKELNRLSLPSKSVDWTHFGGSAPSDETRSLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKTAYRKELEKNRSHVLQQNQRLDTAQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKTEAAEAAEERLDCCRDGCLDSVTGHNNEEASGPNGEAKPPRPGGARGGGGSRGGGGSSGSGSELSTPEKPPNPRAAPLGSRWETAVGEPATGIPVTVGSLPSSKSFLGMKARELFRNKSESQCDEDSLTSSSLSETLKTELGKDSGVEAKPPLNLDGPQPPPPKPDSVGQLRIMDYNEVQHEHS

Target ORF information:

RefSeq Version	XM_007101476.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007101476.2

ORF Insert Sequence:

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_007101476.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007101538.1 Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.

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CloneID	OPh111767
Clone ID Related Accession (Same CDS sequence)	XM_007101478.1 , XM_007101477.1 , XM_007101478.2 , XM_024117344.1 , XM_007101476.2 , XM_007101476.1
Accession Version	XM_007101476.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	hamartin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873644.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007101538.1
CDS	348..3845
Translation	MAQQANIGELLSMLDSPMLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSTLSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTASSYVDTQNSYGSATSTPHSTSRLMLLNTPGQLPQTLSSLSTRLLTEPPQATLWSPSVVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGRKGTPLGTPATSPPPAPPCHSDDYVHISLPQAMATAPKKEERADSARPCLHRQHHLPNDRGLEELPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLAPRGGFDSPFYRDSLPGCPRKTHSAVSSAQGTGANPEPLNSALDRLGPDTPKQAFTPIDLPCGGAEESPAGDRERQASLESSILTPSPFKIPPPRGVGFGGGQPPPYDHLFEVALPKTARHFVSKKTEGLLKKAKGNTEEDCTPSASPMEVLDRLIQQGADAHSKELNRLSLPSKSVDWTHFGGSAPSDETRSLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKTAYRKELEKNRSHVLQQNQRLDTAQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKTEAAEAAEERLDCCRDGCLDSVTGHNNEEASGPNGEAKPPRPGGARGGGGSRGGGGSSGSGSELSTPEKPPNPRAAPLGSRWETAVGEPATGIPVTVGSLPSSKSFLGMKARELFRNKSESQCDEDSLTSSSLSETLKTELGKDSGVEAKPPLNLDGPQPPPPKPDSVGQLRIMDYNEVQHEHS

Target ORF information:

RefSeq Version	XM_007101476.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007101476.1

ORF Insert Sequence:

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGGTG 
CAGCACGAGC ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_028497223.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_028353024.1 Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh320223
Clone ID Related Accession (Same CDS sequence)	XM_028497223.1
Accession Version	XM_028497223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041226.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGCGT 
GCCGAGGAGG GAGTGCAGCT CGCATCCACC ACCTTCGCTA CGCCATCAGG TGTTTGGGGG 
AAGGCAGGAA ACTACCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028353024.1
CDS	348..3908
Translation	MAQQANIGELLSMLDSPMLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAASRLSTLSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTASSYVDTQNSYGSATSTPHSTSRLMLLNTPGQLPQTLSSLSTRLLTEPPQATLWSPSVVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGRKGTPLGTPATSPPPAPPCHSDDYVHISLPQAMATAPKKEERADSARPCLHRQHHLPNDRGLEELPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISKELSEITTADAEPLAPRGGFDSPFYRDSLPGCPRKTHSAVSSAQGTGANPEPLNSALDRLGPDTPKQAFTPIDLPCGGAEESPAGDRERQASLESSILTPSPFKIPPPRGVGFGGGQPPPYDHLFEVALPKTARHFVSKKTEGLLKKAKGNTEEDCTPSASPMEVLDRLIQQGADAHSKELNRLSLPSKSVDWTHFGGSAPSDETRSLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQEQRDTVVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKTAYRKELEKNRSHVLQQNQRLDTAQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKTEAAEAAEERLDCCRDGCLDSVTGHNNEEASGPNGEAKPPRPGGARGGGGSRGGGGSSGSGSELSTPEKPPNPRAAPLGSRWETAVGEPATGIPVTVGSLPSSKSFLGMKARELFRNKSESQCDEDSLTSSSLSETLKTELGKDSGVEAKPPLNLDGPQPPPPKPDSVGQLRIMDYNERAEEGVQLASTTFATPSGVWGKAGNYL

Target ORF information:

RefSeq Version	XM_028497223.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028497223.1

ORF Insert Sequence:

ATGGCCCAAC AAGCAAATAT TGGGGAGCTC CTCTCCATGT TGGATTCCCC CATGCTGAGT 
GTGCGTGATG ATGTAACAGC TGTCTTTAAA GAGAACCTCA GTTCTGACCG TGGTCCGATG 
CTTGTGAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCTGT ATTGCACATC 
CTGACTACCT TGCAAGAGCC ACATGATAAG CACCTCTTGG ACAAAATGAA TGAATACGTG 
GGCAAAGCTG CCTCTCGGTT ATCCACCCTC TCGTTACTGG GCCACGTTGT GAGGCTGCAG 
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC 
AAGATGGACA CCGATGTCAT TGTCCTCACA ACAGGGGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAGTCGGG GAAACAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCAT GGTGCCTGAA GAAACCAGGC CACGTAACGG AAATCTATCT CGTCCACCTC 
CACGCCAGCG TTTACGCTCT CTTCCATCGC CTGTATGGGA TGTACCCTTG CAACTTTGTC 
TCCTTCCTAC GTTCTCACTA CAGTATGAAG GAGAACCTGG AGACTTTTGA AGAGGTGGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAC CCAGAATTAG TGACTGGATC CAAGGACCAT 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTCAT AGAATGTGCC 
AAAATCTCTC TGGACCCTAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA 
ATCTCAGCCC GTTTTCCTCA TCGTTCAGCC GATGTCACCG CCAGCTCTTA TGTTGACACA 
CAGAATAGCT ATGGGAGTGC TACTTCTACC CCTCACTCCA CTTCTCGGCT GATGTTGTTA 
AACACGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCTGT CGACACGGCT GTTAACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT GTGGTTTGTG GGATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC CGATTTGTCA CACCCTTACA GTAAAGTCTT TGGCACAGCG 
GGTAGAAAAG GAACTCCTCT GGGAACCCCT GCAACCTCTC CTCCTCCAGC CCCACCCTGC 
CATTCGGATG ACTACGTGCA CATTTCACTC CCGCAGGCCA TGGCCACAGC CCCCAAGAAG 
GAAGAGAGAG CAGATTCTGC AAGGCCCTGT CTACATAGAC AACACCATCT TCCGAATGAC 
AGAGGATTAG AAGAGCTACC TGGGAGCAAA GGGTCTGTCA CTCTCAGTGA TCTTCCAGGG 
TTTTTAGGTG ACCTGGCTTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCGGCA 
ATATCTAAAG AACTCTCTGA GATCACAACA GCCGACGCCG AGCCCCTAGC TCCTCGCGGA 
GGCTTTGACT CTCCCTTCTA CCGGGACAGT CTCCCGGGCT GCCCGCGGAA GACCCACTCA 
GCCGTCTCCA GTGCCCAGGG CACCGGTGCG AACCCTGAGC CTCTGAACTC CGCCCTGGAC 
AGGCTGGGGC CTGACACCCC AAAGCAAGCC TTTACCCCCA TAGACCTGCC CTGCGGAGGC 
GCCGAGGAGA GCCCTGCCGG GGACAGAGAG CGCCAGGCCT CTCTGGAGAG CAGCATCCTC 
ACTCCCAGCC CTTTTAAAAT TCCACCTCCG AGGGGAGTGG GGTTTGGCGG TGGGCAGCCT 
CCCCCGTACG ATCATCTTTT TGAGGTGGCA CTGCCAAAGA CTGCCCGTCA TTTTGTCAGC 
AAGAAGACGG AGGGACTGCT AAAGAAAGCA AAAGGGAACA CAGAGGAGGA CTGCACGCCC 
TCGGCCTCCC CCATGGAAGT GCTGGACAGA CTGATACAGC AAGGGGCAGA CGCGCACAGC 
AAGGAGCTCA ACAGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTCGGAGGC 
TCCGCTCCCT CAGATGAGAC CCGCTCCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CACGCGCTTC GGAACAGGCG GCTCCTGCGC 
AAGGTGATCA GGGCAGCTGC TCTGGAGGAG CACAACGCTG CCATGAAAGA CCAGTTGAAG 
TTACAAGAGA AAGACATCCA GATATGGAAG GTTAGTCTGC AGAAAGAACA AGCCAGATAC 
AGTCAGCTTC AGGAGCAGCG AGACACGGTG GTCACCCAGC TCCACAGCCA GATCAGACAG 
CTTCAGCACG ACCGGGAGGA GTTCTACAAC CAGAGCCAGG AGTTACAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC GGAGCTGCGC ATAGAACTGA AGAAGGCCAA CAGCAAGGTG 
TGCCACACCG AGCTGCTGCT CAGCCAAGTG TCTCAGAAGC TCTCAAATAG TGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAAGT CAACGAGCTG 
TATTTGGAAC AACTGCAGAA CAAGCATTCA GACACCACAA AGGAAGTAGA AATGATGAAA 
ACTGCATATC GGAAAGAACT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GAATCAGAGG 
CTGGATACTG CTCAAAAACG GATTTTGGAA CTGGAATCTC ATCTAGCCAA GAAAGACCAC 
CTTCTTTTGG AACAGAAAAA ATATTTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG 
CAGGCCGCAG AGAGCAGGTA TGAAGCTCAG AAGAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGCCTCC TGAAAAAACT TGAAGAAGAG 
AAAACAGAAG CAGCCGAAGC AGCGGAAGAA AGGCTCGACT GTTGTCGTGA CGGCTGCTTG 
GATTCTGTGA CAGGGCACAA CAATGAAGAG GCATCTGGCC CTAACGGGGA GGCCAAGCCC 
CCCAGGCCCG GCGGTGCCCG GGGCGGCGGC GGAAGTAGAG GAGGCGGAGG CAGCAGCGGC 
AGCGGCAGTG AGCTGTCTAC CCCGGAGAAA CCCCCAAACC CGAGGGCGGC CCCATTGGGC 
AGTCGGTGGG AGACTGCCGT GGGAGAGCCC GCTACGGGCA TCCCTGTGAC TGTCGGCTCC 
CTTCCCAGTT CAAAAAGCTT CCTGGGCATG AAGGCTCGAG AGCTGTTCCG TAATAAGAGT 
GAGAGCCAGT GTGATGAGGA CAGCCTGACC AGCAGTAGCC TTTCTGAGAC CCTAAAGACA 
GAGCTGGGCA AAGACTCGGG TGTGGAAGCC AAGCCTCCCC TGAACCTAGA CGGCCCGCAG 
CCACCTCCCC CGAAGCCAGA CAGCGTGGGA CAGCTCCGTA TCATGGACTA CAACGAGCGT 
GCCGAGGAGG GAGTGCAGCT CGCATCCACC ACCTTCGCTA CGCCATCAGG TGTTTGGGGG 
AAGGCAGGAA ACTACCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TSC1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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