KNL1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	KNL1
Entrez Gene ID	102988354
Full Name	kinetochore scaffold 1
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007111675.1	XP_007111737.1	LOW QUALITY PROTEIN: protein CASC5
XM_007111675.2	XP_007111737.2	LOW QUALITY PROTEIN: kinetochore scaffold 1

Pathways from BioSystems
Gene Ontology

The following KNL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KNL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPh120411	XM_007111675.1 Latest version!	Physeter catodon cancer susceptibility candidate 5 (CASC5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPh286619	XM_007111675.2 Latest version!	Physeter catodon kinetochore scaffold 1 (KNL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KNL1 ( XM_007111675.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007111737.1 Physeter catodon cancer susceptibility candidate 5 (CASC5), mRNA.

Price & Availability↑

CloneID	OPh120411
Clone ID Related Accession (Same CDS sequence)	XM_007111675.1
Accession Version	XM_007111675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	LOW QUALITY PROTEIN: protein CASC5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006714149.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TGTCTTCAGA GGCTAATGAA GAAAATGACA ATGTAGAGAG ACCTGTTAGA 
AGACGACATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT TAGAGGTGGG 
AATGGGAATG AAACTGTTCA GGAATCCAAT GCTTTGAGAA ATAAGAAAAA CTCTCGTCGA 
GTCAGCTTTG CAGATACTAT AAAAGTATTC CAGACAGACC CTCATATGAA AATAGTGAAA 
AAGTCAGAAA TTTCAGAAAC AGAAGCAGGA GGAAATGTCC TATTTATGCA GAATAAGAAT 
CCAGAAGATA ACTACTGTGA GATAACTGGG ATGAATACAT TGCTTTGTGC TCCAATTCAG 
ACCCAGATGC CCCAGAGAGA GATTTCAATG ACAGAATACA ACCATGAAAG GAAACATGCA 
AATGACCAGA CAGTCATCTT TTCAGATGAA AACCAGATGG ACCTGACAGC AAGTCACACT 
GTGATGATTA CCAAAGGCCT TTTAGATTGT ACCAAAAGTG AAAAATCCAC CAAGATAGAT 
ACCACATCAT TTCTGGCTAA CTTAAAGCTC CACATTGAGG ATGGAAGAAT GAAGAAAGAA 
TTAAATTTTT CTGGGGATCA AAGCACTTCT TCAGAAAAGA AAATAAATTT CAATGACTTT 
GTAAAAAGAT TGAAAACAGG AAAATCGAAT GCTTCTCCTT TTACAGGACC TGATAAAGAA 
AATTCTGAGA TGCCTATTCA TTACATAGAA TCAAATAGGG CCTCTTCTGT GCATCAAATG 
CACGTATCTC TTAGTGTAGA TGAAAATAGC AGTAATATGA CTAGACTCTT TAGAGAACAA 
GACAATGGGA TGAATTTTAC CCAGTGTCAT ACAGCCAATA TTCAGACTTC GGTTCCCACA 
GCCAGTGAGC CCAGCTTAAG GGAATTTAAA GGTGATGATA TTACAATTTA TGGCAATGAC 
TTTATGGACC TGACAATTAA CCACACTATA CAGAAGTTAC CTTCAGCAGA TAATCTGTCT 
GAAATAGAAA ATCAAACTCA GAATGTCATG CTGGATGTTT CAACAGGTCA TGGAGCTAAA 
GCACTAGGAC AGAAGACAGT CTTTAAGGAT AAACCGAACG ATGCTTTTCA AGACCCTTCC 
TTAAACCCTG AAGGTGACAT ACATATTACC AGCAGTCATA TTACAGGGAC AGAAATTCAG 
ACAGTCACAC AGACTTCCAA CCAGGATGTC AGAACATTGG CCATGATCCC AGAATCTAAA 
CGTTCCAGTC CACCTATTCA AGATTATAAG ACATTTTTCT GTTCTAGTTG TAATGATGCC 
ATGGAACTGA CCAAGTGTTT CTCAAGTATG AGAGAGGAGA AGAATTTGCT AAAGCATGAC 
AGGAGTTATT CTAAAATGTA TCCCAATCCA GATGCCATCT CTCTTCTCAG AGAGAATACG 
ATTTATTCAG GAGAAGAAAG CATGGACATT ACCAAGAGTC ACACAGTTGC AATAGATAAT 
CAAATTTTTA AACAAGTTCA GACAAGTGTA CAGATAGCAG CTGCACCAGT ATCTGAAAAA 
GAAATGATGA TCCAAAATCA CATAATCATG TCAGAGGATG GGGAAATGAA TATAAATTAT 
AGCTCAGTTC CTCATGTATC TAAGGAAAGA TTACAGCAGA GCCTGGAAAA TCCTTTATTT 
ATTTCATTGA CTGACAAAAG GACTGAAATC TTCACGGATG AAAATATGGA TTTGACTAAA 
AGTCACATAG CTAATGTAGG AAGTCAGTTT CCTCTTGCAT CTTACAGTCT GGCATCTGAG 
TATACCAGTA AATCTCACTC TCACAGCATA AGCCCTTCAG ATGAATGGGA AGAAATGACC 
AAAGGTCATA TTGAACCATC CCGACAACCA AGCATAATAT CTAAAAACAT CCCAGCTGAC 
ACATGGGACA AAGGAAAAGA TCAAGTTTTG AATATTTCAC CCTATCTTGA TAAAGATTCT 
CCTCAGTCAC CTGATATTAA CCAGAACATA GCAACAAGCC ATAATATAGT ATACTCTGGT 
GGAGATCTTG ATAAACAAGT ACCACTGAGA AATAATAGAA ATACAGTTTC TTGTGAGCAA 
TCTTTGTTTC CTACTACAAA GCCATTGTTT TCATCAGAAG GACAGTCTGC CATGAGAAAT 
CATAATACTG CTGTTAACAG TCAAACAGTG AAATCTGTAT CAGACCAGAA TTCTAAACTG 
CCTGAGCCAC TGAGGAAAAG TTTAGGCAGT CACACGCCTG ACTGTTTTCA TGACAAGATA 
ATTATTTATT CAGAGAAGGA GCAAAATATG GACCTAACCA AGAGTCACAC TGTTGTCATT 
GGATTTGGTC CTTCTGAAGT ACTAGAACTA GGTAAGACTA ATTTAGAAAA TAATAACAAC 
CAGCTAACAA CAGTAAACAG ACAGAAAGCT GTAAAAATTG AAAAACGTAA TGAAAGTCCA 
ATAGAAGAAA ATGGAGTATT TATTTCTAAT GATATCATGG GTGTGTTGAA GGACAAAACT 
GGAATTCTGA ATGAAAAGCA AGATGTCAAA ATTTGTGAAA GGAAAAGTCT TGGCAGACTA 
AAAATTGATA AGACTGTTGT ATTTTCAGAG GGAGATGAGA ATGATATGGA TATCACCAAG 
AGTTACACAG TGGAAATAAA TCATAGACCT TTACTAGATG AACATGATTC CCATTTGGTG 
CCTTTGGCAG AAACTTCTAA AACTGTTTTG TATACATGTG AGCAGGATGA CATGGAGGTC 
ACCAGAAGTC ACACAACTGC TATAGAATGT AAAACTATCT CACCAGATAA AATAACTACT 
AGGCCTGTGG ACAAAACTGT AATGTTTATA GATAATCACG AAGAAGTAGA GATGACAAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA AAGGAGAAAA CTGTGCTTCC AGACAGCCCT 
AACTTTGAAC TATCCAAAAG AAAAAGCCTA GAAAAATCAA AAGTGACACC TAATCCTGCT 
AAGGAGGGTG TTTTCTTTCT GGACAATGGT GAAAGTGATC ATTCAGTAGC AAAAGTCAGC 
CAGCTAGCAT TACCAGGGGA ATGGTCTAAT AGTGATCCTT TAGAGAAAGC TGAAGCATTT 
ATAGCTGATG ATAACGTGGA AGTGTCTAAA TCAACCATTT GGAAAAATGA CACAGATGTA 
CAAAAGCCTG GATTTCTGAA TGAATCTCTA TCAGGCAAAA GTCAGAGAAG GAAGAGCCTT 
AGACTAAAAA ATGACAAGAC TATTGCATTT TCAAAGAATG ACAGAAATGG TATGGATGTC 
ATCCAGAGTT GTACAGTGGA AATAAATAAC AAAGGTGTCC TGGAAGATAG AGAGGACTCC 
CATTGGGTGC CTTTGGAAGG AACTTCTAAT ACTGTTTTAT ATACATGTGG GCAGGATGAT 
ATGGAGATTA CCAGAAGTCA TACAACTGCC TTAGAATATA AACCTCTCTC ACCAGATAAA 
AGAACCACTG GGCCTATGGA CAAAACTGTT ATGTTTGTAG ATAATCACAG TGATCTGGAA 
GTTACTAAGT CCCACACTGT TTTCATTGAT TGTCAAGCCA TGGAGAAAAT ACTTCAAGAG 
TACCCTAAAT TTGGAATAGC GAAAGGAAAA ACCTTGGGTG TTTCTTTTCC TAAGGATGGT 
AGCTCTGTTC AAGAAATCAC TAAAGAACAA GCACTGGCTG TAGAAAACAA AATTGTTCTT 
CACACTGAGC AAAAGCATCA TGTGATACCC TTTGTTCCTA CTAATACATT GTCTGGGGGT 
CAAGGTGAGA CAGAAATCAA ATTCCATAGC ACTGCTGTGG ATGAAGAGGT CATGAAAGTT 
GTAGTCCAGG CCTATGTATT GGAAAAAGCC AAAATTGAAA GCTGTCACTT AAATAGTACA 
GATAGAAGAA ATGTGGGTTT TACAGGCAGT TCTGCAGCTG CTATTTGTGG ATCCAGTGAT 
AATTATTCCT GTTTACCTAA TGTTTCCTTT TTTGATAATT TGGAGGAGAA TGGCATGCCC 
TTGTGTGATA AAGATGATGT AAAAGCCAGT CATTGCCCAG TGCAAAATGA TCTTCCTTAT 
GCAAACAATT TAGCTAGTGA ATATTATTTG AAATCTGAGG GACTGCCTCT TTCTGCTCCT 
TGTTCTTTGT TAGAGAAGGA AAAAGTTGTT CAAACCAGTA CCCAAGGACA GTTAGACTGT 
GTCCTAACAG TACTCAAAGA TCAAGATCTG ATTAAGGAGC CTCCAAATCT GTTAGCTAAT 
GAAACTTTAA TATATGGTGA AGATTTGGGG GAGATGACTA AACTTAACTC AAAGCAGGTA 
TCTTGTAAGC TTCCAAAGGA TCAAATGGAG GCCTTTGTTG ATAATGCAGA GCATAGTTTA 
AAGAAGACCT TTGTTGATGA TGTTTGTGTT GCTTCTCAGC ATCATCTGTC AACCCAGCAA 
CATCCATTAC CTCAACAAGG ACAGAGTATT GTCAGTAAAG ATGAAACAAT ATTGTCTAAA 
GTGGGAAATA AGAATTTAAA TATTGTTAGA GGAAATTCCT CTGAACCCAT ATGTGAAAAC 
GATTCCACAA TGCTCAATAA TGAGAAACAG TTTACTATGG CCTGTAAAAA AGAACTAAAG 
AAAAATATTC AAACAGCTAA ATGTAATACA GCTATAGATT TCCACAGTAA CTCAGACTTG 
ACTAAGCAAG TTATTCAAAC TCATATCAAT CCTAGAGAAG CATCAGATCC TGTAATTGCA 
TCTAATGCTG CAACTTTTAG TAGTATCAAA CCACATCTGA ATAATTTGAA TGAAAAAACT 
GAAGAATTTT TAGATTTCCA ACCAGTTCAT TTACCACCTT CTCCAGAACA ATTACTTGAA 
TTAGTAAATA AGGCACACAG TAATATGAGT ATAGTGCAAG CTACAGAAAT ACATAATGTT 
AACATAGTGT CCAGCAGTGT TAAAGATGAT AGGGATGAAG AAAATAAAAT TTCTCATAAT 
GGAGCTGAAA CTATCTCTGT ACCATTAATG ACGACTATAA AAGATAAAGT GAGGAGATGT 
TCTTTGGGAA TATTTTTGCC CAGATTGCCA AACAAGAGAA ATTGTAGTGT CACTGGTATT 
GATGACCTGA AGCCGATTCC AGGAGACACA ACTGATTTAA ATAACTTAGA AACTCAACCA 
GTCTCCAGTA AACATTCAGA CACTGGATTT GTGGCAGCTA AACTGAACTT AAGTCCATCT 
CAATATATAA ATGAGGAAAA TCTTCCTATA TATCCGGGTG AGATTAATTC CTCAGACTCT 
ATTAGCATAG ATACTGAAGA AAAGGGTTTG ACTGAGACAT ACCAAAAAGA GATTTCACCA 
TCTGAAAATA AACTGGAAGA AACCTGCAAT AGCCAAAAAA GAACCTGGGT ACAAGAAGAA 
GAAGATGATA TTCAGGATGA GAAAAAAATC AGAAAAAGTG AAATTAGGTT TAGTGATACC 
GCACAAGATC AGGAGATTTT TGATCACCGT GCTGAAGGAG CTATAGATAA AAATGCTAAC 
AGTGTGTTGA TAAAAAGCCT GAGCAGGACC CCATCTAGTT GCAGCAGTTC TCTGGATTCA 
GTCAAGGCTG ATGGGACCTC TCTGGACTTC AGCACACACC CCAGTAGTCA AATAGAATCA 
CAGTTCCTCA GAGATACCGT TTGTGAAGAG AGCTTGAAGG AGAAACTCAA AGATGGGAGA 
ATAACAATAA AGGAGTTCTT TATACTTCTT CAGGTCCACA TTCTAATACA AAAACCTCGA 
CAGAGCAATC TCCCAACCAA ATTTACTATA AACACATCAC CTACTCCAGA AGACCTGATG 
TTAAGTCAAT ATGTTTACCG ACCCAAGATA CAGATTTATA GAGAAGATTG TGAAGCTCTT 
CGCCAGAAGA TTGAAGAATT AAAGCTTTCT GCACTGAACC AAGATAAACT GTTCACTGAT 
GTAAATAGGA ACCTATGGGA AAAAGTGAGA CACTACTCTG ATGAGGAGCT GAAGGCCTTT 
GGAATTTACC TGAACAAAAT AAAATCGCGT TTTACCAAGA TGACTAAAGT CTTCACTCAC 
CAAGGAAAAG TGTCCCTGTA CAGCAAGCTG GTACGGTCGG CTCAGAACGA GAGGGAGAAG 
CTTCAGACAA GGATAAATGA GATGGACAAC ATATTTCAGA AGATTGATAA CTGTCTCACT 
GAGGTGGAAA TAGAAACTAA AAATTTGGAG GATGAACAGA AAGACAATCC TGTGGAAGAG 
TGGGATTCTG AAATGAAGGC TGCAGAGAAA GAATTGGAAC AGCTGAAAAC TGAAGAACAA 
AAGCTTCAAA GAAATCTCTT AGAGCTGGAG GTACAGAAAA AGCAGACCCT TGCTCAAATA 
GACTTTGTGC AAAAACAAAC AAATAGAACT GAAGAGCTAC TGGATCATTT GAGCTTGTCT 
GAATGGGATG TCATTGAGTG GAGTGATGAT CAAGCTATAT TCACCTTTCT TTATGACACA 
GTAGAACTCA CCATCACCTT TGGAGAGCCA GTAGTTGGTC TCCGTTTCCT GGACAAGGCT 
TGTAGGAAGA TTGTTGACCT GAATTTTCAA TCTCTGTTAG ATGAGGATAA AGCTCCTCCT 
TCCTCCCTTT TAGTTCATAA CCTTATTTTC CAGTACATTG AGCAACAGCA ATCCTGGAAG 
AAGAAATGTG CAACACAGCA TCAGGTACCC AAGATGCTTC AAGAAATCTC ACTGGTAGTG 
AGCCATTGCA AACTTCTTGG AGAGGAGATT GAGTTTTTAA AGCGATGGGG ACCAAATTAT 
AACCTAATGA ACATAGCTGT GAATAATACT GAATTGAGGC TTTTATTCTG CAGCTCAGCA 
GCATTTGCAA AGTTTGAGAT AACTTTGTCT CTCTCAGCCC ATTACCCATC TGTCCCATTA 
CCTTTCTCTA TTCAAAATCA CCTTGGAAAC ATTGGCCAAG ATGAAATCAC TGCCATTCTC 
TCTAAAGCGC CACTGGAAGA CAACTACCTA AAGAATGTAG TCAAGCAAAT TTACCAAGAT 
CTGCTTCAGA CTGACTGCCA TTTCTACCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007111737.1
CDS	139..7191
Translation	MDGMSSEANEENDNVERPVRRRHSSILKPPRSPLQDLRGGNGNETVQESNALRNKKNSRRVSFADTIKVFQTDPHMKIVKKSEISETEAGGNVLFMQNKNPEDNYCEITGMNTLLCAPIQTQMPQREISMTEYNHERKHANDQTVIFSDENQMDLTASHTVMITKGLLDCTKSEKSTKIDTTSFLANLKLHIEDGRMKKELNFSGDQSTSSEKKINFNDFVKRLKTGKSNASPFTGPDKENSEMPIHYIESNRASSVHQMHVSLSVDENSSNMTRLFREQDNGMNFTQCHTANIQTSVPTASEPSLREFKGDDITIYGNDFMDLTINHTIQKLPSADNLSEIENQTQNVMLDVSTGHGAKALGQKTVFKDKPNDAFQDPSLNPEGDIHITSSHITGTEIQTVTQTSNQDVRTLAMIPESKRSSPPIQDYKTFFCSSCNDAMELTKCFSSMREEKNLLKHDRSYSKMYPNPDAISLLRENTIYSGEESMDITKSHTVAIDNQIFKQVQTSVQIAAAPVSEKEMMIQNHIIMSEDGEMNINYSSVPHVSKERLQQSLENPLFISLTDKRTEIFTDENMDLTKSHIANVGSQFPLASYSLASEYTSKSHSHSISPSDEWEEMTKGHIEPSRQPSIISKNIPADTWDKGKDQVLNISPYLDKDSPQSPDINQNIATSHNIVYSGGDLDKQVPLRNNRNTVSCEQSLFPTTKPLFSSEGQSAMRNHNTAVNSQTVKSVSDQNSKLPEPLRKSLGSHTPDCFHDKIIIYSEKEQNMDLTKSHTVVIGFGPSEVLELGKTNLENNNNQLTTVNRQKAVKIEKRNESPIEENGVFISNDIMGVLKDKTGILNEKQDVKICERKSLGRLKIDKTVVFSEGDENDMDITKSYTVEINHRPLLDEHDSHLVPLAETSKTVLYTCEQDDMEVTRSHTTAIECKTISPDKITTRPVDKTVMFIDNHEEVEMTKSHTVFIDYQAKEKTVLPDSPNFELSKRKSLEKSKVTPNPAKEGVFFLDNGESDHSVAKVSQLALPGEWSNSDPLEKAEAFIADDNVEVSKSTIWKNDTDVQKPGFLNESLSGKSQRRKSLRLKNDKTIAFSKNDRNGMDVIQSCTVEINNKGVLEDREDSHWVPLEGTSNTVLYTCGQDDMEITRSHTTALEYKPLSPDKRTTGPMDKTVMFVDNHSDLEVTKSHTVFIDCQAMEKILQEYPKFGIAKGKTLGVSFPKDGSSVQEITKEQALAVENKIVLHTEQKHHVIPFVPTNTLSGGQGETEIKFHSTAVDEEVMKVVVQAYVLEKAKIESCHLNSTDRRNVGFTGSSAAAICGSSDNYSCLPNVSFFDNLEENGMPLCDKDDVKASHCPVQNDLPYANNLASEYYLKSEGLPLSAPCSLLEKEKVVQTSTQGQLDCVLTVLKDQDLIKEPPNLLANETLIYGEDLGEMTKLNSKQVSCKLPKDQMEAFVDNAEHSLKKTFVDDVCVASQHHLSTQQHPLPQQGQSIVSKDETILSKVGNKNLNIVRGNSSEPICENDSTMLNNEKQFTMACKKELKKNIQTAKCNTAIDFHSNSDLTKQVIQTHINPREASDPVIASNAATFSSIKPHLNNLNEKTEEFLDFQPVHLPPSPEQLLELVNKAHSNMSIVQATEIHNVNIVSSSVKDDRDEENKISHNGAETISVPLMTTIKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLKPIPGDTTDLNNLETQPVSSKHSDTGFVAAKLNLSPSQYINEENLPIYPGEINSSDSISIDTEEKGLTETYQKEISPSENKLEETCNSQKRTWVQEEEDDIQDEKKIRKSEIRFSDTAQDQEIFDHRAEGAIDKNANSVLIKSLSRTPSSCSSSLDSVKADGTSLDFSTHPSSQIESQFLRDTVCEESLKEKLKDGRITIKEFFILLQVHILIQKPRQSNLPTKFTINTSPTPEDLMLSQYVYRPKIQIYREDCEALRQKIEELKLSALNQDKLFTDVNRNLWEKVRHYSDEELKAFGIYLNKIKSRFTKMTKVFTHQGKVSLYSKLVRSAQNEREKLQTRINEMDNIFQKIDNCLTEVEIETKNLEDEQKDNPVEEWDSEMKAAEKELEQLKTEEQKLQRNLLELEVQKKQTLAQIDFVQKQTNRTEELLDHLSLSEWDVIEWSDDQAIFTFLYDTVELTITFGEPVVGLRFLDKACRKIVDLNFQSLLDEDKAPPSSLLVHNLIFQYIEQQQSWKKKCATQHQVPKMLQEISLVVSHCKLLGEEIEFLKRWGPNYNLMNIAVNNTELRLLFCSSAAFAKFEITLSLSAHYPSVPLPFSIQNHLGNIGQDEITAILSKAPLEDNYLKNVVKQIYQDLLQTDCHFYQ

Target ORF information:

RefSeq Version	XM_007111675.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon cancer susceptibility candidate 5 (CASC5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007111675.1

ORF Insert Sequence:

ATGGATGGGA TGTCTTCAGA GGCTAATGAA GAAAATGACA ATGTAGAGAG ACCTGTTAGA 
AGACGACATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT TAGAGGTGGG 
AATGGGAATG AAACTGTTCA GGAATCCAAT GCTTTGAGAA ATAAGAAAAA CTCTCGTCGA 
GTCAGCTTTG CAGATACTAT AAAAGTATTC CAGACAGACC CTCATATGAA AATAGTGAAA 
AAGTCAGAAA TTTCAGAAAC AGAAGCAGGA GGAAATGTCC TATTTATGCA GAATAAGAAT 
CCAGAAGATA ACTACTGTGA GATAACTGGG ATGAATACAT TGCTTTGTGC TCCAATTCAG 
ACCCAGATGC CCCAGAGAGA GATTTCAATG ACAGAATACA ACCATGAAAG GAAACATGCA 
AATGACCAGA CAGTCATCTT TTCAGATGAA AACCAGATGG ACCTGACAGC AAGTCACACT 
GTGATGATTA CCAAAGGCCT TTTAGATTGT ACCAAAAGTG AAAAATCCAC CAAGATAGAT 
ACCACATCAT TTCTGGCTAA CTTAAAGCTC CACATTGAGG ATGGAAGAAT GAAGAAAGAA 
TTAAATTTTT CTGGGGATCA AAGCACTTCT TCAGAAAAGA AAATAAATTT CAATGACTTT 
GTAAAAAGAT TGAAAACAGG AAAATCGAAT GCTTCTCCTT TTACAGGACC TGATAAAGAA 
AATTCTGAGA TGCCTATTCA TTACATAGAA TCAAATAGGG CCTCTTCTGT GCATCAAATG 
CACGTATCTC TTAGTGTAGA TGAAAATAGC AGTAATATGA CTAGACTCTT TAGAGAACAA 
GACAATGGGA TGAATTTTAC CCAGTGTCAT ACAGCCAATA TTCAGACTTC GGTTCCCACA 
GCCAGTGAGC CCAGCTTAAG GGAATTTAAA GGTGATGATA TTACAATTTA TGGCAATGAC 
TTTATGGACC TGACAATTAA CCACACTATA CAGAAGTTAC CTTCAGCAGA TAATCTGTCT 
GAAATAGAAA ATCAAACTCA GAATGTCATG CTGGATGTTT CAACAGGTCA TGGAGCTAAA 
GCACTAGGAC AGAAGACAGT CTTTAAGGAT AAACCGAACG ATGCTTTTCA AGACCCTTCC 
TTAAACCCTG AAGGTGACAT ACATATTACC AGCAGTCATA TTACAGGGAC AGAAATTCAG 
ACAGTCACAC AGACTTCCAA CCAGGATGTC AGAACATTGG CCATGATCCC AGAATCTAAA 
CGTTCCAGTC CACCTATTCA AGATTATAAG ACATTTTTCT GTTCTAGTTG TAATGATGCC 
ATGGAACTGA CCAAGTGTTT CTCAAGTATG AGAGAGGAGA AGAATTTGCT AAAGCATGAC 
AGGAGTTATT CTAAAATGTA TCCCAATCCA GATGCCATCT CTCTTCTCAG AGAGAATACG 
ATTTATTCAG GAGAAGAAAG CATGGACATT ACCAAGAGTC ACACAGTTGC AATAGATAAT 
CAAATTTTTA AACAAGTTCA GACAAGTGTA CAGATAGCAG CTGCACCAGT ATCTGAAAAA 
GAAATGATGA TCCAAAATCA CATAATCATG TCAGAGGATG GGGAAATGAA TATAAATTAT 
AGCTCAGTTC CTCATGTATC TAAGGAAAGA TTACAGCAGA GCCTGGAAAA TCCTTTATTT 
ATTTCATTGA CTGACAAAAG GACTGAAATC TTCACGGATG AAAATATGGA TTTGACTAAA 
AGTCACATAG CTAATGTAGG AAGTCAGTTT CCTCTTGCAT CTTACAGTCT GGCATCTGAG 
TATACCAGTA AATCTCACTC TCACAGCATA AGCCCTTCAG ATGAATGGGA AGAAATGACC 
AAAGGTCATA TTGAACCATC CCGACAACCA AGCATAATAT CTAAAAACAT CCCAGCTGAC 
ACATGGGACA AAGGAAAAGA TCAAGTTTTG AATATTTCAC CCTATCTTGA TAAAGATTCT 
CCTCAGTCAC CTGATATTAA CCAGAACATA GCAACAAGCC ATAATATAGT ATACTCTGGT 
GGAGATCTTG ATAAACAAGT ACCACTGAGA AATAATAGAA ATACAGTTTC TTGTGAGCAA 
TCTTTGTTTC CTACTACAAA GCCATTGTTT TCATCAGAAG GACAGTCTGC CATGAGAAAT 
CATAATACTG CTGTTAACAG TCAAACAGTG AAATCTGTAT CAGACCAGAA TTCTAAACTG 
CCTGAGCCAC TGAGGAAAAG TTTAGGCAGT CACACGCCTG ACTGTTTTCA TGACAAGATA 
ATTATTTATT CAGAGAAGGA GCAAAATATG GACCTAACCA AGAGTCACAC TGTTGTCATT 
GGATTTGGTC CTTCTGAAGT ACTAGAACTA GGTAAGACTA ATTTAGAAAA TAATAACAAC 
CAGCTAACAA CAGTAAACAG ACAGAAAGCT GTAAAAATTG AAAAACGTAA TGAAAGTCCA 
ATAGAAGAAA ATGGAGTATT TATTTCTAAT GATATCATGG GTGTGTTGAA GGACAAAACT 
GGAATTCTGA ATGAAAAGCA AGATGTCAAA ATTTGTGAAA GGAAAAGTCT TGGCAGACTA 
AAAATTGATA AGACTGTTGT ATTTTCAGAG GGAGATGAGA ATGATATGGA TATCACCAAG 
AGTTACACAG TGGAAATAAA TCATAGACCT TTACTAGATG AACATGATTC CCATTTGGTG 
CCTTTGGCAG AAACTTCTAA AACTGTTTTG TATACATGTG AGCAGGATGA CATGGAGGTC 
ACCAGAAGTC ACACAACTGC TATAGAATGT AAAACTATCT CACCAGATAA AATAACTACT 
AGGCCTGTGG ACAAAACTGT AATGTTTATA GATAATCACG AAGAAGTAGA GATGACAAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA AAGGAGAAAA CTGTGCTTCC AGACAGCCCT 
AACTTTGAAC TATCCAAAAG AAAAAGCCTA GAAAAATCAA AAGTGACACC TAATCCTGCT 
AAGGAGGGTG TTTTCTTTCT GGACAATGGT GAAAGTGATC ATTCAGTAGC AAAAGTCAGC 
CAGCTAGCAT TACCAGGGGA ATGGTCTAAT AGTGATCCTT TAGAGAAAGC TGAAGCATTT 
ATAGCTGATG ATAACGTGGA AGTGTCTAAA TCAACCATTT GGAAAAATGA CACAGATGTA 
CAAAAGCCTG GATTTCTGAA TGAATCTCTA TCAGGCAAAA GTCAGAGAAG GAAGAGCCTT 
AGACTAAAAA ATGACAAGAC TATTGCATTT TCAAAGAATG ACAGAAATGG TATGGATGTC 
ATCCAGAGTT GTACAGTGGA AATAAATAAC AAAGGTGTCC TGGAAGATAG AGAGGACTCC 
CATTGGGTGC CTTTGGAAGG AACTTCTAAT ACTGTTTTAT ATACATGTGG GCAGGATGAT 
ATGGAGATTA CCAGAAGTCA TACAACTGCC TTAGAATATA AACCTCTCTC ACCAGATAAA 
AGAACCACTG GGCCTATGGA CAAAACTGTT ATGTTTGTAG ATAATCACAG TGATCTGGAA 
GTTACTAAGT CCCACACTGT TTTCATTGAT TGTCAAGCCA TGGAGAAAAT ACTTCAAGAG 
TACCCTAAAT TTGGAATAGC GAAAGGAAAA ACCTTGGGTG TTTCTTTTCC TAAGGATGGT 
AGCTCTGTTC AAGAAATCAC TAAAGAACAA GCACTGGCTG TAGAAAACAA AATTGTTCTT 
CACACTGAGC AAAAGCATCA TGTGATACCC TTTGTTCCTA CTAATACATT GTCTGGGGGT 
CAAGGTGAGA CAGAAATCAA ATTCCATAGC ACTGCTGTGG ATGAAGAGGT CATGAAAGTT 
GTAGTCCAGG CCTATGTATT GGAAAAAGCC AAAATTGAAA GCTGTCACTT AAATAGTACA 
GATAGAAGAA ATGTGGGTTT TACAGGCAGT TCTGCAGCTG CTATTTGTGG ATCCAGTGAT 
AATTATTCCT GTTTACCTAA TGTTTCCTTT TTTGATAATT TGGAGGAGAA TGGCATGCCC 
TTGTGTGATA AAGATGATGT AAAAGCCAGT CATTGCCCAG TGCAAAATGA TCTTCCTTAT 
GCAAACAATT TAGCTAGTGA ATATTATTTG AAATCTGAGG GACTGCCTCT TTCTGCTCCT 
TGTTCTTTGT TAGAGAAGGA AAAAGTTGTT CAAACCAGTA CCCAAGGACA GTTAGACTGT 
GTCCTAACAG TACTCAAAGA TCAAGATCTG ATTAAGGAGC CTCCAAATCT GTTAGCTAAT 
GAAACTTTAA TATATGGTGA AGATTTGGGG GAGATGACTA AACTTAACTC AAAGCAGGTA 
TCTTGTAAGC TTCCAAAGGA TCAAATGGAG GCCTTTGTTG ATAATGCAGA GCATAGTTTA 
AAGAAGACCT TTGTTGATGA TGTTTGTGTT GCTTCTCAGC ATCATCTGTC AACCCAGCAA 
CATCCATTAC CTCAACAAGG ACAGAGTATT GTCAGTAAAG ATGAAACAAT ATTGTCTAAA 
GTGGGAAATA AGAATTTAAA TATTGTTAGA GGAAATTCCT CTGAACCCAT ATGTGAAAAC 
GATTCCACAA TGCTCAATAA TGAGAAACAG TTTACTATGG CCTGTAAAAA AGAACTAAAG 
AAAAATATTC AAACAGCTAA ATGTAATACA GCTATAGATT TCCACAGTAA CTCAGACTTG 
ACTAAGCAAG TTATTCAAAC TCATATCAAT CCTAGAGAAG CATCAGATCC TGTAATTGCA 
TCTAATGCTG CAACTTTTAG TAGTATCAAA CCACATCTGA ATAATTTGAA TGAAAAAACT 
GAAGAATTTT TAGATTTCCA ACCAGTTCAT TTACCACCTT CTCCAGAACA ATTACTTGAA 
TTAGTAAATA AGGCACACAG TAATATGAGT ATAGTGCAAG CTACAGAAAT ACATAATGTT 
AACATAGTGT CCAGCAGTGT TAAAGATGAT AGGGATGAAG AAAATAAAAT TTCTCATAAT 
GGAGCTGAAA CTATCTCTGT ACCATTAATG ACGACTATAA AAGATAAAGT GAGGAGATGT 
TCTTTGGGAA TATTTTTGCC CAGATTGCCA AACAAGAGAA ATTGTAGTGT CACTGGTATT 
GATGACCTGA AGCCGATTCC AGGAGACACA ACTGATTTAA ATAACTTAGA AACTCAACCA 
GTCTCCAGTA AACATTCAGA CACTGGATTT GTGGCAGCTA AACTGAACTT AAGTCCATCT 
CAATATATAA ATGAGGAAAA TCTTCCTATA TATCCGGGTG AGATTAATTC CTCAGACTCT 
ATTAGCATAG ATACTGAAGA AAAGGGTTTG ACTGAGACAT ACCAAAAAGA GATTTCACCA 
TCTGAAAATA AACTGGAAGA AACCTGCAAT AGCCAAAAAA GAACCTGGGT ACAAGAAGAA 
GAAGATGATA TTCAGGATGA GAAAAAAATC AGAAAAAGTG AAATTAGGTT TAGTGATACC 
GCACAAGATC AGGAGATTTT TGATCACCGT GCTGAAGGAG CTATAGATAA AAATGCTAAC 
AGTGTGTTGA TAAAAAGCCT GAGCAGGACC CCATCTAGTT GCAGCAGTTC TCTGGATTCA 
GTCAAGGCTG ATGGGACCTC TCTGGACTTC AGCACACACC CCAGTAGTCA AATAGAATCA 
CAGTTCCTCA GAGATACCGT TTGTGAAGAG AGCTTGAAGG AGAAACTCAA AGATGGGAGA 
ATAACAATAA AGGAGTTCTT TATACTTCTT CAGGTCCACA TTCTAATACA AAAACCTCGA 
CAGAGCAATC TCCCAACCAA ATTTACTATA AACACATCAC CTACTCCAGA AGACCTGATG 
TTAAGTCAAT ATGTTTACCG ACCCAAGATA CAGATTTATA GAGAAGATTG TGAAGCTCTT 
CGCCAGAAGA TTGAAGAATT AAAGCTTTCT GCACTGAACC AAGATAAACT GTTCACTGAT 
GTAAATAGGA ACCTATGGGA AAAAGTGAGA CACTACTCTG ATGAGGAGCT GAAGGCCTTT 
GGAATTTACC TGAACAAAAT AAAATCGCGT TTTACCAAGA TGACTAAAGT CTTCACTCAC 
CAAGGAAAAG TGTCCCTGTA CAGCAAGCTG GTACGGTCGG CTCAGAACGA GAGGGAGAAG 
CTTCAGACAA GGATAAATGA GATGGACAAC ATATTTCAGA AGATTGATAA CTGTCTCACT 
GAGGTGGAAA TAGAAACTAA AAATTTGGAG GATGAACAGA AAGACAATCC TGTGGAAGAG 
TGGGATTCTG AAATGAAGGC TGCAGAGAAA GAATTGGAAC AGCTGAAAAC TGAAGAACAA 
AAGCTTCAAA GAAATCTCTT AGAGCTGGAG GTACAGAAAA AGCAGACCCT TGCTCAAATA 
GACTTTGTGC AAAAACAAAC AAATAGAACT GAAGAGCTAC TGGATCATTT GAGCTTGTCT 
GAATGGGATG TCATTGAGTG GAGTGATGAT CAAGCTATAT TCACCTTTCT TTATGACACA 
GTAGAACTCA CCATCACCTT TGGAGAGCCA GTAGTTGGTC TCCGTTTCCT GGACAAGGCT 
TGTAGGAAGA TTGTTGACCT GAATTTTCAA TCTCTGTTAG ATGAGGATAA AGCTCCTCCT 
TCCTCCCTTT TAGTTCATAA CCTTATTTTC CAGTACATTG AGCAACAGCA ATCCTGGAAG 
AAGAAATGTG CAACACAGCA TCAGGTACCC AAGATGCTTC AAGAAATCTC ACTGGTAGTG 
AGCCATTGCA AACTTCTTGG AGAGGAGATT GAGTTTTTAA AGCGATGGGG ACCAAATTAT 
AACCTAATGA ACATAGCTGT GAATAATACT GAATTGAGGC TTTTATTCTG CAGCTCAGCA 
GCATTTGCAA AGTTTGAGAT AACTTTGTCT CTCTCAGCCC ATTACCCATC TGTCCCATTA 
CCTTTCTCTA TTCAAAATCA CCTTGGAAAC ATTGGCCAAG ATGAAATCAC TGCCATTCTC 
TCTAAAGCGC CACTGGAAGA CAACTACCTA AAGAATGTAG TCAAGCAAAT TTACCAAGAT 
CTGCTTCAGA CTGACTGCCA TTTCTACCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNL1 ( XM_007111675.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007111737.2 Physeter catodon kinetochore scaffold 1 (KNL1), mRNA.

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CloneID	OPh286619
Clone ID Related Accession (Same CDS sequence)	XM_007111675.2
Accession Version	XM_007111675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	LOW QUALITY PROTEIN: kinetochore scaffold 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041224.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 23, 2018 this sequence version replaced XM_007111675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGATGGGA TGTCTTCAGA GGCTAATGAA GAAAATGACA ATGTAGAGAG ACCTGTTAGA 
AGACGACATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT TAGAGGTGGG 
AATGGGAATG AAACTGTTCA GGAATCCAAT GCTTTGAGAA ATAAGAAAAA CTCTCGTCGA 
GTCAGCTTTG CAGATACTAT AAAAGTATTC CAGACAGACC CTCATATGAA AATAGTGAAA 
AAGTCAGAAA TTTCAGAAAC AGAAGCAGGA GGAAATGTCC TATTTATGCA GAATAAGAAT 
CCAGAAGATA ACTACTGTGA GATAACTGGG ATGAATACAT TGCTTTGTGC TCCAATTCAG 
ACCCAGATGC CCCAGAGAGA GATTTCAATG ACAGAATACA ACCATGAAAG TAAACATGCA 
AATGACCAGA CAGTCATCTT TTCAGATGAA AACCAGATGG ACCTGACAGC AAGTCACACT 
GTGATGATTA CCAAAGGCCT TTTAGATTGT ACCAAAAGTG AAAAATCCAC CAAGATAGAT 
ACCACATCAT TTCTGGCTAA CTTAAAGCTC CACATTGAGG ATGGAAGAAT GAAGAAAGAA 
TTAAATTTTT CTGGGGATCA AAGCACTTCT TCAGAAAAGA AAATAAATTT CAATGACTTT 
GTAAAAAGAT TGAAAACAGG AAAATCGAAT GCTTCTCCTT TTACAGGACC TGATAAAGAA 
AATTCTGAGA TGCCTATTCA TTACATAGAA TCAAATAGGG CCTCTTCTGT GCATCAAATG 
CACGTATCTC TTAGTGTAGA TGAAAATAGC AGTAATATGA CTAGACTCTT TAGAGAACAA 
GACAATGGGA TGAATTTTAC CCAGTGTCAT ACAGCCAATA TTCAGACTTC GGTTCCCACA 
GCCAGTGAGC CCAGCTTAAG GGAATTTAAA GGTGATGATA TTACAATTTA TGGCAATGAC 
TTTATGGACC TGACAATTAA CCACACTATA CAGAAGTTAC CTTCAGCAGA TAATCTGTCT 
GAAATAGAAA ATCAAACTCA GAATGTCATG CTGGATGTTT CAACAGGTCA TGGAGCTAAA 
GCACTAGGAC AGAAGACAGT CTTTAAGGAT AAACCGAACG ATGCTTTTCA AGACCCTTCC 
TTAAACCCTG AAGGTGACAT ACATATTACC AGCAGTCATA TTACAGGGAC AGAAATTCAG 
ACAGTCACAC AGACTTCCAA CCAGGATGTC AGAACATTGG CCATGATCCC AGAATCTAAA 
CGTTCCAGTC CACCTATTCA AGATTATAAG ACATTTTTCT GTTCTAGTTG TAATGATGCC 
ATGGAACTGA CCAAGTGTTT CTCAAGTATG AGAGAGGAGA AGAATTTGCT AAAGCATGAC 
AGGAGTTATT CTAAAATGTA TCCCAATCCA GATGCCATCT CTCTTCTCAG AGAGAATACG 
ATTTATTCAG GAGAAGAAAG CATGGACATT ACCAAGAGTC ACACAGTTGC AATAGATAAT 
CAAATTTTTA AACAAGTTCA GACAAGTGTA CAGATAGCAG CTGCACCAGT ATCTGAAAAA 
GAAATGATGA TCCAAAATCA CATAATCATG TCAGAGGATG GGGAAATGAA TATAAATTAT 
AGCTCAGTTC CTCATGTATC TAAGGAAAGA TTACAGCAGA GCCTGGAAAA TCCTCTATTT 
ATTTCATTGA CTGACAAAAG GACTGAAATC TTCACGGATG AAAATATGGA TTTGACTAAA 
AGTCACATAG CTAATGTAGG AAGTCAGTTT CCTCTTGCAT CTTACAGTCT GGCATCTGAG 
TATACCAGTA AATCTCACTC TCACAGCATA AGCCCTTCAG ATGAATGGGA AGAAATGACC 
AAAGGTCATA TTGAACCATC CCGACAACCA AGCATAATAT CTAAAAACAT CCCAGCTGAC 
ACATGGGACA AAGGAAAAGA TCAAGTTTTG AATATTTCAC CCTATCTTGA TAAAGATTCT 
CCTCAGTCAC CTGATATTAA CCAGAACATA GCAACAAGCC ATAATATAGT ATACTCTGGT 
GGAGATCTTG ATAAACAAGT ACCACTGAGA AATAATAGAA ATACAGTTTC TTGTGAGCAA 
TCTTTGTTTC CTACTACAAA GCCATTGTTT TCATCAGAAG GACAGTCTGC CATGAGAAAT 
CATAATACTG CTGTTAACAG TCAAACAGTG AAATCTGTAT CAGACCAGAA TTCTAAACTG 
CCTGAGCCAC TGAGGAAAAG TTTAGGCAGT CACACGCCTG ACTGTTTTCA TGACAAGATA 
ATTATTTATT CAGAGAAGGA GCAAAATATG GACCTAACCA AGAGTCACAC TGTTGTCATT 
GGATTTGGTC CTTCTGAAGT ACTAGAACTA GGTAAGACTA ATTTAGAAAA TAATAACAAC 
CAGCTAACAA CAGTAAACAG ACAGAAAGCT GTAAAAATTG AAAAACGTAA TGAAAGTCCA 
ATAGAAGAAA ATGGAGTATT TATTTCTAAT GATATCATGG GTGTGTTGAA GGACAAAACT 
GGAATTCTGA ATGAAAAGCA AGATGTCAAA ATTTGTGAAA GGAAAAGTCT TGGCAGACTA 
AAAATTGATA AGACTGTTGT ATTTTCAGAG GGAGATGAGA ATGATATGGA TATCACCAAG 
AGTTACACAG TGGAAATAAA TCATAGACCT TTACTAGATG AACATAATTC CCATTTGGTG 
CCTTTGGCAG AAACTTCTAA AACTGTTTTG TATACATGTG AGCAGGATGA CATGGAGGTC 
ACCAGAAGTC ACACAACTGC TATAGAATGT AAAACTATCT CACCAGATAA AATAACTACT 
AGGCCTGTGG ACAAAACTGT AATGTTTATA GATAATCACG AAGAAGTAGA GATGACAAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA AAGGAGAAAA CTGTGCTTCC AGACAGCCCT 
AACTTTGAAC TACCCAAAAG AAAAAGCCTA GAAAAATCAA AAGTGACACC TAATCCTGCT 
AAGGAGGGTG TTTTCTTTCT GGACAATGGT GAAAGTGATC ATTCAGTAGC AAAAGTCAGC 
CAGCTAGCAT TACCAGGGGA ATGGTCTAAT AGTGATCCTT TAGAGAAAGC TGAAGCATTT 
ATAGCTGATG ATAACGTGGA AGTGTCTAAA TCAACCATTT GGAAAAATGA CACAGATGTA 
CAAAAGCCTG GATTTCTGAA TGAATCTCTA TCAGGCAAAA GTCAGAGAAG GAAGAGCCTT 
AGACTAAAAA ATGACAAGAC TATTGCATTT TCAAAGAATG ACAGAAATGG TATGGATGTC 
ATCCAGAGTT GTACAGTGGA AATAAATAAC AAAGGTGTCC TGGAAGATAG AGAGGACTCC 
CATTGGGTGC CTTTGGAAGG AACTTCTAAT ACTGTTTTAT ATACATGTGG GCAGGATGAT 
ATGGAGATTA CCAGAAGTCA TACAACTGCC TTAGAATATA AACCTCTCTC ACCAGATAAA 
AGAACCACTG GGCCTATGGA CAAAACTGTT ATGTTTGTAG ATAATCACAG TGATCTGGAA 
GTTACTAAGT CCCACACTGT TTTCATTGAT TGTCAAGCCA TGGAGAAAAT ACTTCAAGAG 
TACCCTAAAT TTGGAATAGC GAAAGGAAAA ACCTTGGGTG TTTCTTTTCC TAAGGATGGT 
AGCTCTGTTC AAGAAATCAC TAAAGAACAA GCACTGGCTG TAGAAAACAA AATTGTTCTT 
CACACTGAGC AAAAGCATCA TGTGATACCC TTTGTTCCTA CTAATACATT GTCTGGGGGT 
CAAGGTGAGA CAGAAATCAA ATTCCATAGC ACTGCTGTGG ATGAAGAGGT CATGAAAGTC 
GTAGTCCAGG CCTATGTATT GGAAAAAGCC AAAATTGAAA GCTGTCACTT AAATAGTACA 
GATAGAAGAA ATGTGGGTTT TACAGGCAGT TCTGCAGCTG CTATTTGTGG ATCCAGTGAT 
AATTATTCCT GTTTACCTAA TGTTTCCTTT TTTGATAATT TGGAGGAGAA TGGCATGCCC 
TTGTGTGATA AAGATGATGT AAAAGCCAGT CATTGCCCAG TGCAAAATGA TCTTCCTTAT 
GCAAACAATT TAGCTAGTGA ATATTATTTG AAATCTGAGG GACTGCCTCT TTCTGCTCCT 
TGTTCTTTGT TAGAGAAGGA AAAAGTTGTT CAAACCAGTA CCCAAGGACA GTTAGACTGT 
GTCCTAACAG TACTCAAAGA TCAAGATCTG ATTAAGGAGC CTCCAAATCT GTTAGCTAAT 
GAAACTTTAA TATATGGTGA AGATTTGGGG GAGATGACTA AACTTAACTC AAAGCAGGTA 
TCTTGTAAGC TTCCAAAGGA TCAAATGGAG GCCTTTGTTG ATAATGCAGA GCATAGTTTA 
AAGAAGACCT TTGTTGATGA TGTTTGTGTT GCTTCTCAGC ATCATCTGTC AACCCAGCAA 
CATCCATTAC CTCAACAAGG ACAGAGTATT GTCAGTAAAG ATGAAACAAT ATTGTCTAAA 
GTGGGAAATA AGAATTTAAA TATTGTTAGA GGAAATTCCT CTGAACCCAT ATGTGAAAAC 
GATTCCACAA TGCTCAATAA TGAGAAACAG TTTACTATGG CCTGTAAAAA AGAACTAAAG 
AAAAATATTC AAACAGCTAA ATGTAATACA GCTATAGATT TCCACAGTAA CTCAGACTTG 
ACTAAGCAAG TTATTCAAAC TCATATCAAT CCTAGAGAAG CATCAGATCC TGTAATTGCA 
TCTAATGCTG CAACTTTTAG TAGTATCAAA CCACATCTGA ATAATTTGAA TGAAAAAACT 
GAAGAATTTT TAGATTTCCA ACCAGTTCAT TTACCACCTT CTCCAGAACA ATTACTTGAA 
TTAGTAAATA AGGCACACAG TAATATGAGT ATAGTGCAAG CTACAGAAAT ACATAATGTT 
AACATAGTGT CCAGCAGTGT TAAAGATGAT AGGGATGAAG AAAATAAAAT TTCTCATAAT 
GGAGCTGAAA CTATCTCTGT ACCATTAATG ACGACTATAA AAGATAAAGT GAGGAGATGT 
TCTTTGGGAA TATTTTTGCC CAGATTGCCA AACAAGAGAA ATTGTAGTGT CACTGGTATT 
GATGACCTGA AGCCGATTCC AGGAGACACA ACTGATTTAA ATAACTTAGA AACTCAACCA 
GTCTCCAGTA AACATTCAGA CACTGGATTT GTGGCAGCTA AACTGAACTT AAGTCCATCT 
CAATATATAA ATGAGGAAAA TCTTCCTATA TATCCGGGTG AGATTAATTC CTCAGACTCT 
ATTAGCATAG ATACTGAAGA AAAGGGTTTG ACTGAGACAT ACCAAAAAGA GATTTCACCA 
TCTGAAAATA AACTGGAAGA AACCTGCAAT AGCCAAAAAA GAACCTGGGT ACAAGAAGAA 
GAAGATGATA TTCAGGATGA GAAAAAAATC AGAAAAAGTG AAATTAGGTT TAGTGATACC 
GCACAAGATC AGGAGATTTT TGATCACCGT GCTGAAGGAG CTATAGATAA AAATGCTAAC 
AGTGTGTTGA TAAAAAGCCT GAGCAGGACC CCATCTAGTT GCAGCAGTTC TCTGGATTCA 
GTCAAGGCTG ATGGGACCTC TCTGGACTTC AGCACACACC CCAGTAGTCA AATAGAATCA 
CAGTTCCTCA GAGATACCGT TTGTGAAGAG AGCTTGAAGG AGAAACTCAA AGATGGGAGA 
ATAACAATAA AGGAGTTCTT TATACTTCTT CAGGTCCACA TTCTAATACA AAAACCTCGA 
CAGAGCAATC TCCCAACCAA ATTTACTATA AACACATCAC CTACTCCAGA AGACCTGATG 
TTAAGTCAAT ATGTTTACCG ACCCAAGATA CAGATTTATA GAGAAGATTG TGAAGCTCTT 
CGCCAGAAGA TTGAAGAATT AAAGCTTTCT GCACTGAACC AAGATAAACT GTTCACTGAT 
GTAAATAGGA ACCTATGGGA AAAAGTGAGA CACTACTCTG ATGAGGAGCT GAAGGCCTTT 
GGAATTTACC TGAACAAAAT AAAATCGCGT TTTACCAAGA TGACTAAAGT CTTCACTCAC 
CAAGGAAAAG TGTCCCTGTA CAGCAAGCTG GTACGGTCGG CTCAGAACGA GAGGGAGAAG 
CTTCAGACAA GGATAAATGA GATGGACAAC ATATTTCAGA AGATTGATAA CTGTCTCACT 
GAGGTGGAAA TAGAAACTAA AAATTTGGAG GATGAACAGA AAGACAATCC TGTGGAAGAG 
TGGGATTCTG AAATGAAGGC TGCAGAGAAA GAATTGGAAC AGCTGAAAAC TGAAGAACAA 
AAGCTTCAAA GAAATCTCTT AGAGCTGGAG GTACAGAAAA AGCAGACCCT TGCTCAAATA 
GACTTTGTGC AAAAACAAAC AAATAGAACT GAAGAGCTAC TGGATCATTT GAGCTTGTCT 
GAATGGGATG TCATTGAGTG GAGTGATGAT CAAGCTATAT TCACCTTTCT TTATGACACA 
GTAGAACTCA CCATCACCTT TGGAGAGCCA GTAGTTGGTC TCCGTTTCCT GGACAAGGCT 
TGTAGGAAGA TTGTTGACCT GAATTTTCAA TCTCTGTTAG ATGAGGATAA AGCTCCTCCT 
TCCTCCCTTT TAGTTCATAA CCTTATTTTC CAGTACATTG AGCAACAGCA ATCCTGGAAG 
AAGAAATGTG CAACACAGCA TCAGGTACCC AAGATGCTTC AAGAAATCTC ACTGGTAGTG 
AGCCATTGCA AACTTCTTGG AGAGGAGATT GAGTTTTTAA AGCGATGGGG ACCAAATTAT 
AACCTAATGA ACATAGCTGT GAATAATACT GAATTGAGGC TTTTATTCTG CAGCTCAGCA 
GCATTTGCAA AGTTTGAGAT AACTTTGTCT CTCTCAGCCC ATTACCCATC TGTCCCATTA 
CCTTTCTCTA TTCAAAATCA CCTTGGAAAC ATTGGCCAAG ATGAAATCAC TGCCATTCTC 
TCTAAAGCGC CACTGGAAGA CAACTACCTA AAGAATGTAG TCAAGCAAAT TTACCAAGAT 
CTGCTTCAGG ACGACTGCCA TTTCTACCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007111737.2
CDS	139..7191
Translation	MDGMSSEANEENDNVERPVRRRHSSILKPPRSPLQDLRGGNGNETVQESNALRNKKNSRRVSFADTIKVFQTDPHMKIVKKSEISETEAGGNVLFMQNKNPEDNYCEITGMNTLLCAPIQTQMPQREISMTEYNHESKHANDQTVIFSDENQMDLTASHTVMITKGLLDCTKSEKSTKIDTTSFLANLKLHIEDGRMKKELNFSGDQSTSSEKKINFNDFVKRLKTGKSNASPFTGPDKENSEMPIHYIESNRASSVHQMHVSLSVDENSSNMTRLFREQDNGMNFTQCHTANIQTSVPTASEPSLREFKGDDITIYGNDFMDLTINHTIQKLPSADNLSEIENQTQNVMLDVSTGHGAKALGQKTVFKDKPNDAFQDPSLNPEGDIHITSSHITGTEIQTVTQTSNQDVRTLAMIPESKRSSPPIQDYKTFFCSSCNDAMELTKCFSSMREEKNLLKHDRSYSKMYPNPDAISLLRENTIYSGEESMDITKSHTVAIDNQIFKQVQTSVQIAAAPVSEKEMMIQNHIIMSEDGEMNINYSSVPHVSKERLQQSLENPLFISLTDKRTEIFTDENMDLTKSHIANVGSQFPLASYSLASEYTSKSHSHSISPSDEWEEMTKGHIEPSRQPSIISKNIPADTWDKGKDQVLNISPYLDKDSPQSPDINQNIATSHNIVYSGGDLDKQVPLRNNRNTVSCEQSLFPTTKPLFSSEGQSAMRNHNTAVNSQTVKSVSDQNSKLPEPLRKSLGSHTPDCFHDKIIIYSEKEQNMDLTKSHTVVIGFGPSEVLELGKTNLENNNNQLTTVNRQKAVKIEKRNESPIEENGVFISNDIMGVLKDKTGILNEKQDVKICERKSLGRLKIDKTVVFSEGDENDMDITKSYTVEINHRPLLDEHNSHLVPLAETSKTVLYTCEQDDMEVTRSHTTAIECKTISPDKITTRPVDKTVMFIDNHEEVEMTKSHTVFIDYQAKEKTVLPDSPNFELPKRKSLEKSKVTPNPAKEGVFFLDNGESDHSVAKVSQLALPGEWSNSDPLEKAEAFIADDNVEVSKSTIWKNDTDVQKPGFLNESLSGKSQRRKSLRLKNDKTIAFSKNDRNGMDVIQSCTVEINNKGVLEDREDSHWVPLEGTSNTVLYTCGQDDMEITRSHTTALEYKPLSPDKRTTGPMDKTVMFVDNHSDLEVTKSHTVFIDCQAMEKILQEYPKFGIAKGKTLGVSFPKDGSSVQEITKEQALAVENKIVLHTEQKHHVIPFVPTNTLSGGQGETEIKFHSTAVDEEVMKVVVQAYVLEKAKIESCHLNSTDRRNVGFTGSSAAAICGSSDNYSCLPNVSFFDNLEENGMPLCDKDDVKASHCPVQNDLPYANNLASEYYLKSEGLPLSAPCSLLEKEKVVQTSTQGQLDCVLTVLKDQDLIKEPPNLLANETLIYGEDLGEMTKLNSKQVSCKLPKDQMEAFVDNAEHSLKKTFVDDVCVASQHHLSTQQHPLPQQGQSIVSKDETILSKVGNKNLNIVRGNSSEPICENDSTMLNNEKQFTMACKKELKKNIQTAKCNTAIDFHSNSDLTKQVIQTHINPREASDPVIASNAATFSSIKPHLNNLNEKTEEFLDFQPVHLPPSPEQLLELVNKAHSNMSIVQATEIHNVNIVSSSVKDDRDEENKISHNGAETISVPLMTTIKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLKPIPGDTTDLNNLETQPVSSKHSDTGFVAAKLNLSPSQYINEENLPIYPGEINSSDSISIDTEEKGLTETYQKEISPSENKLEETCNSQKRTWVQEEEDDIQDEKKIRKSEIRFSDTAQDQEIFDHRAEGAIDKNANSVLIKSLSRTPSSCSSSLDSVKADGTSLDFSTHPSSQIESQFLRDTVCEESLKEKLKDGRITIKEFFILLQVHILIQKPRQSNLPTKFTINTSPTPEDLMLSQYVYRPKIQIYREDCEALRQKIEELKLSALNQDKLFTDVNRNLWEKVRHYSDEELKAFGIYLNKIKSRFTKMTKVFTHQGKVSLYSKLVRSAQNEREKLQTRINEMDNIFQKIDNCLTEVEIETKNLEDEQKDNPVEEWDSEMKAAEKELEQLKTEEQKLQRNLLELEVQKKQTLAQIDFVQKQTNRTEELLDHLSLSEWDVIEWSDDQAIFTFLYDTVELTITFGEPVVGLRFLDKACRKIVDLNFQSLLDEDKAPPSSLLVHNLIFQYIEQQQSWKKKCATQHQVPKMLQEISLVVSHCKLLGEEIEFLKRWGPNYNLMNIAVNNTELRLLFCSSAAFAKFEITLSLSAHYPSVPLPFSIQNHLGNIGQDEITAILSKAPLEDNYLKNVVKQIYQDLLQDDCHFYQ

Target ORF information:

RefSeq Version	XM_007111675.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon kinetochore scaffold 1 (KNL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007111675.2

ORF Insert Sequence:

ATGGATGGGA TGTCTTCAGA GGCTAATGAA GAAAATGACA ATGTAGAGAG ACCTGTTAGA 
AGACGACATT CCTCAATTTT GAAACCCCCA AGGAGTCCTC TTCAGGACCT TAGAGGTGGG 
AATGGGAATG AAACTGTTCA GGAATCCAAT GCTTTGAGAA ATAAGAAAAA CTCTCGTCGA 
GTCAGCTTTG CAGATACTAT AAAAGTATTC CAGACAGACC CTCATATGAA AATAGTGAAA 
AAGTCAGAAA TTTCAGAAAC AGAAGCAGGA GGAAATGTCC TATTTATGCA GAATAAGAAT 
CCAGAAGATA ACTACTGTGA GATAACTGGG ATGAATACAT TGCTTTGTGC TCCAATTCAG 
ACCCAGATGC CCCAGAGAGA GATTTCAATG ACAGAATACA ACCATGAAAG TAAACATGCA 
AATGACCAGA CAGTCATCTT TTCAGATGAA AACCAGATGG ACCTGACAGC AAGTCACACT 
GTGATGATTA CCAAAGGCCT TTTAGATTGT ACCAAAAGTG AAAAATCCAC CAAGATAGAT 
ACCACATCAT TTCTGGCTAA CTTAAAGCTC CACATTGAGG ATGGAAGAAT GAAGAAAGAA 
TTAAATTTTT CTGGGGATCA AAGCACTTCT TCAGAAAAGA AAATAAATTT CAATGACTTT 
GTAAAAAGAT TGAAAACAGG AAAATCGAAT GCTTCTCCTT TTACAGGACC TGATAAAGAA 
AATTCTGAGA TGCCTATTCA TTACATAGAA TCAAATAGGG CCTCTTCTGT GCATCAAATG 
CACGTATCTC TTAGTGTAGA TGAAAATAGC AGTAATATGA CTAGACTCTT TAGAGAACAA 
GACAATGGGA TGAATTTTAC CCAGTGTCAT ACAGCCAATA TTCAGACTTC GGTTCCCACA 
GCCAGTGAGC CCAGCTTAAG GGAATTTAAA GGTGATGATA TTACAATTTA TGGCAATGAC 
TTTATGGACC TGACAATTAA CCACACTATA CAGAAGTTAC CTTCAGCAGA TAATCTGTCT 
GAAATAGAAA ATCAAACTCA GAATGTCATG CTGGATGTTT CAACAGGTCA TGGAGCTAAA 
GCACTAGGAC AGAAGACAGT CTTTAAGGAT AAACCGAACG ATGCTTTTCA AGACCCTTCC 
TTAAACCCTG AAGGTGACAT ACATATTACC AGCAGTCATA TTACAGGGAC AGAAATTCAG 
ACAGTCACAC AGACTTCCAA CCAGGATGTC AGAACATTGG CCATGATCCC AGAATCTAAA 
CGTTCCAGTC CACCTATTCA AGATTATAAG ACATTTTTCT GTTCTAGTTG TAATGATGCC 
ATGGAACTGA CCAAGTGTTT CTCAAGTATG AGAGAGGAGA AGAATTTGCT AAAGCATGAC 
AGGAGTTATT CTAAAATGTA TCCCAATCCA GATGCCATCT CTCTTCTCAG AGAGAATACG 
ATTTATTCAG GAGAAGAAAG CATGGACATT ACCAAGAGTC ACACAGTTGC AATAGATAAT 
CAAATTTTTA AACAAGTTCA GACAAGTGTA CAGATAGCAG CTGCACCAGT ATCTGAAAAA 
GAAATGATGA TCCAAAATCA CATAATCATG TCAGAGGATG GGGAAATGAA TATAAATTAT 
AGCTCAGTTC CTCATGTATC TAAGGAAAGA TTACAGCAGA GCCTGGAAAA TCCTCTATTT 
ATTTCATTGA CTGACAAAAG GACTGAAATC TTCACGGATG AAAATATGGA TTTGACTAAA 
AGTCACATAG CTAATGTAGG AAGTCAGTTT CCTCTTGCAT CTTACAGTCT GGCATCTGAG 
TATACCAGTA AATCTCACTC TCACAGCATA AGCCCTTCAG ATGAATGGGA AGAAATGACC 
AAAGGTCATA TTGAACCATC CCGACAACCA AGCATAATAT CTAAAAACAT CCCAGCTGAC 
ACATGGGACA AAGGAAAAGA TCAAGTTTTG AATATTTCAC CCTATCTTGA TAAAGATTCT 
CCTCAGTCAC CTGATATTAA CCAGAACATA GCAACAAGCC ATAATATAGT ATACTCTGGT 
GGAGATCTTG ATAAACAAGT ACCACTGAGA AATAATAGAA ATACAGTTTC TTGTGAGCAA 
TCTTTGTTTC CTACTACAAA GCCATTGTTT TCATCAGAAG GACAGTCTGC CATGAGAAAT 
CATAATACTG CTGTTAACAG TCAAACAGTG AAATCTGTAT CAGACCAGAA TTCTAAACTG 
CCTGAGCCAC TGAGGAAAAG TTTAGGCAGT CACACGCCTG ACTGTTTTCA TGACAAGATA 
ATTATTTATT CAGAGAAGGA GCAAAATATG GACCTAACCA AGAGTCACAC TGTTGTCATT 
GGATTTGGTC CTTCTGAAGT ACTAGAACTA GGTAAGACTA ATTTAGAAAA TAATAACAAC 
CAGCTAACAA CAGTAAACAG ACAGAAAGCT GTAAAAATTG AAAAACGTAA TGAAAGTCCA 
ATAGAAGAAA ATGGAGTATT TATTTCTAAT GATATCATGG GTGTGTTGAA GGACAAAACT 
GGAATTCTGA ATGAAAAGCA AGATGTCAAA ATTTGTGAAA GGAAAAGTCT TGGCAGACTA 
AAAATTGATA AGACTGTTGT ATTTTCAGAG GGAGATGAGA ATGATATGGA TATCACCAAG 
AGTTACACAG TGGAAATAAA TCATAGACCT TTACTAGATG AACATAATTC CCATTTGGTG 
CCTTTGGCAG AAACTTCTAA AACTGTTTTG TATACATGTG AGCAGGATGA CATGGAGGTC 
ACCAGAAGTC ACACAACTGC TATAGAATGT AAAACTATCT CACCAGATAA AATAACTACT 
AGGCCTGTGG ACAAAACTGT AATGTTTATA GATAATCACG AAGAAGTAGA GATGACAAAG 
TCCCATACTG TTTTCATTGA CTACCAAGCA AAGGAGAAAA CTGTGCTTCC AGACAGCCCT 
AACTTTGAAC TACCCAAAAG AAAAAGCCTA GAAAAATCAA AAGTGACACC TAATCCTGCT 
AAGGAGGGTG TTTTCTTTCT GGACAATGGT GAAAGTGATC ATTCAGTAGC AAAAGTCAGC 
CAGCTAGCAT TACCAGGGGA ATGGTCTAAT AGTGATCCTT TAGAGAAAGC TGAAGCATTT 
ATAGCTGATG ATAACGTGGA AGTGTCTAAA TCAACCATTT GGAAAAATGA CACAGATGTA 
CAAAAGCCTG GATTTCTGAA TGAATCTCTA TCAGGCAAAA GTCAGAGAAG GAAGAGCCTT 
AGACTAAAAA ATGACAAGAC TATTGCATTT TCAAAGAATG ACAGAAATGG TATGGATGTC 
ATCCAGAGTT GTACAGTGGA AATAAATAAC AAAGGTGTCC TGGAAGATAG AGAGGACTCC 
CATTGGGTGC CTTTGGAAGG AACTTCTAAT ACTGTTTTAT ATACATGTGG GCAGGATGAT 
ATGGAGATTA CCAGAAGTCA TACAACTGCC TTAGAATATA AACCTCTCTC ACCAGATAAA 
AGAACCACTG GGCCTATGGA CAAAACTGTT ATGTTTGTAG ATAATCACAG TGATCTGGAA 
GTTACTAAGT CCCACACTGT TTTCATTGAT TGTCAAGCCA TGGAGAAAAT ACTTCAAGAG 
TACCCTAAAT TTGGAATAGC GAAAGGAAAA ACCTTGGGTG TTTCTTTTCC TAAGGATGGT 
AGCTCTGTTC AAGAAATCAC TAAAGAACAA GCACTGGCTG TAGAAAACAA AATTGTTCTT 
CACACTGAGC AAAAGCATCA TGTGATACCC TTTGTTCCTA CTAATACATT GTCTGGGGGT 
CAAGGTGAGA CAGAAATCAA ATTCCATAGC ACTGCTGTGG ATGAAGAGGT CATGAAAGTC 
GTAGTCCAGG CCTATGTATT GGAAAAAGCC AAAATTGAAA GCTGTCACTT AAATAGTACA 
GATAGAAGAA ATGTGGGTTT TACAGGCAGT TCTGCAGCTG CTATTTGTGG ATCCAGTGAT 
AATTATTCCT GTTTACCTAA TGTTTCCTTT TTTGATAATT TGGAGGAGAA TGGCATGCCC 
TTGTGTGATA AAGATGATGT AAAAGCCAGT CATTGCCCAG TGCAAAATGA TCTTCCTTAT 
GCAAACAATT TAGCTAGTGA ATATTATTTG AAATCTGAGG GACTGCCTCT TTCTGCTCCT 
TGTTCTTTGT TAGAGAAGGA AAAAGTTGTT CAAACCAGTA CCCAAGGACA GTTAGACTGT 
GTCCTAACAG TACTCAAAGA TCAAGATCTG ATTAAGGAGC CTCCAAATCT GTTAGCTAAT 
GAAACTTTAA TATATGGTGA AGATTTGGGG GAGATGACTA AACTTAACTC AAAGCAGGTA 
TCTTGTAAGC TTCCAAAGGA TCAAATGGAG GCCTTTGTTG ATAATGCAGA GCATAGTTTA 
AAGAAGACCT TTGTTGATGA TGTTTGTGTT GCTTCTCAGC ATCATCTGTC AACCCAGCAA 
CATCCATTAC CTCAACAAGG ACAGAGTATT GTCAGTAAAG ATGAAACAAT ATTGTCTAAA 
GTGGGAAATA AGAATTTAAA TATTGTTAGA GGAAATTCCT CTGAACCCAT ATGTGAAAAC 
GATTCCACAA TGCTCAATAA TGAGAAACAG TTTACTATGG CCTGTAAAAA AGAACTAAAG 
AAAAATATTC AAACAGCTAA ATGTAATACA GCTATAGATT TCCACAGTAA CTCAGACTTG 
ACTAAGCAAG TTATTCAAAC TCATATCAAT CCTAGAGAAG CATCAGATCC TGTAATTGCA 
TCTAATGCTG CAACTTTTAG TAGTATCAAA CCACATCTGA ATAATTTGAA TGAAAAAACT 
GAAGAATTTT TAGATTTCCA ACCAGTTCAT TTACCACCTT CTCCAGAACA ATTACTTGAA 
TTAGTAAATA AGGCACACAG TAATATGAGT ATAGTGCAAG CTACAGAAAT ACATAATGTT 
AACATAGTGT CCAGCAGTGT TAAAGATGAT AGGGATGAAG AAAATAAAAT TTCTCATAAT 
GGAGCTGAAA CTATCTCTGT ACCATTAATG ACGACTATAA AAGATAAAGT GAGGAGATGT 
TCTTTGGGAA TATTTTTGCC CAGATTGCCA AACAAGAGAA ATTGTAGTGT CACTGGTATT 
GATGACCTGA AGCCGATTCC AGGAGACACA ACTGATTTAA ATAACTTAGA AACTCAACCA 
GTCTCCAGTA AACATTCAGA CACTGGATTT GTGGCAGCTA AACTGAACTT AAGTCCATCT 
CAATATATAA ATGAGGAAAA TCTTCCTATA TATCCGGGTG AGATTAATTC CTCAGACTCT 
ATTAGCATAG ATACTGAAGA AAAGGGTTTG ACTGAGACAT ACCAAAAAGA GATTTCACCA 
TCTGAAAATA AACTGGAAGA AACCTGCAAT AGCCAAAAAA GAACCTGGGT ACAAGAAGAA 
GAAGATGATA TTCAGGATGA GAAAAAAATC AGAAAAAGTG AAATTAGGTT TAGTGATACC 
GCACAAGATC AGGAGATTTT TGATCACCGT GCTGAAGGAG CTATAGATAA AAATGCTAAC 
AGTGTGTTGA TAAAAAGCCT GAGCAGGACC CCATCTAGTT GCAGCAGTTC TCTGGATTCA 
GTCAAGGCTG ATGGGACCTC TCTGGACTTC AGCACACACC CCAGTAGTCA AATAGAATCA 
CAGTTCCTCA GAGATACCGT TTGTGAAGAG AGCTTGAAGG AGAAACTCAA AGATGGGAGA 
ATAACAATAA AGGAGTTCTT TATACTTCTT CAGGTCCACA TTCTAATACA AAAACCTCGA 
CAGAGCAATC TCCCAACCAA ATTTACTATA AACACATCAC CTACTCCAGA AGACCTGATG 
TTAAGTCAAT ATGTTTACCG ACCCAAGATA CAGATTTATA GAGAAGATTG TGAAGCTCTT 
CGCCAGAAGA TTGAAGAATT AAAGCTTTCT GCACTGAACC AAGATAAACT GTTCACTGAT 
GTAAATAGGA ACCTATGGGA AAAAGTGAGA CACTACTCTG ATGAGGAGCT GAAGGCCTTT 
GGAATTTACC TGAACAAAAT AAAATCGCGT TTTACCAAGA TGACTAAAGT CTTCACTCAC 
CAAGGAAAAG TGTCCCTGTA CAGCAAGCTG GTACGGTCGG CTCAGAACGA GAGGGAGAAG 
CTTCAGACAA GGATAAATGA GATGGACAAC ATATTTCAGA AGATTGATAA CTGTCTCACT 
GAGGTGGAAA TAGAAACTAA AAATTTGGAG GATGAACAGA AAGACAATCC TGTGGAAGAG 
TGGGATTCTG AAATGAAGGC TGCAGAGAAA GAATTGGAAC AGCTGAAAAC TGAAGAACAA 
AAGCTTCAAA GAAATCTCTT AGAGCTGGAG GTACAGAAAA AGCAGACCCT TGCTCAAATA 
GACTTTGTGC AAAAACAAAC AAATAGAACT GAAGAGCTAC TGGATCATTT GAGCTTGTCT 
GAATGGGATG TCATTGAGTG GAGTGATGAT CAAGCTATAT TCACCTTTCT TTATGACACA 
GTAGAACTCA CCATCACCTT TGGAGAGCCA GTAGTTGGTC TCCGTTTCCT GGACAAGGCT 
TGTAGGAAGA TTGTTGACCT GAATTTTCAA TCTCTGTTAG ATGAGGATAA AGCTCCTCCT 
TCCTCCCTTT TAGTTCATAA CCTTATTTTC CAGTACATTG AGCAACAGCA ATCCTGGAAG 
AAGAAATGTG CAACACAGCA TCAGGTACCC AAGATGCTTC AAGAAATCTC ACTGGTAGTG 
AGCCATTGCA AACTTCTTGG AGAGGAGATT GAGTTTTTAA AGCGATGGGG ACCAAATTAT 
AACCTAATGA ACATAGCTGT GAATAATACT GAATTGAGGC TTTTATTCTG CAGCTCAGCA 
GCATTTGCAA AGTTTGAGAT AACTTTGTCT CTCTCAGCCC ATTACCCATC TGTCCCATTA 
CCTTTCTCTA TTCAAAATCA CCTTGGAAAC ATTGGCCAAG ATGAAATCAC TGCCATTCTC 
TCTAAAGCGC CACTGGAAGA CAACTACCTA AAGAATGTAG TCAAGCAAAT TTACCAAGAT 
CTGCTTCAGG ACGACTGCCA TTTCTACCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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KNL1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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