TOP2A cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP2A
Entrez Gene ID	102980935
Full Name	DNA topoisomerase II alpha
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007112498.3	XP_007112560.2	DNA topoisomerase 2-alpha
XM_007112498.1	XP_007112560.1	DNA topoisomerase 2-alpha
XM_007112498.2	XP_007112560.2	DNA topoisomerase 2-alpha

Pathways from BioSystems
Gene Ontology

The following TOP2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh121096	XM_007112498.1 Latest version!	Physeter catodon topoisomerase (DNA) II alpha 170kDa (TOP2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPh298989	XM_007112498.3 Latest version!	Physeter catodon DNA topoisomerase II alpha (TOP2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OPh298989	XM_007112498.2	Physeter catodon DNA topoisomerase II alpha (TOP2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP2A ( XM_007112498.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007112560.1 Physeter catodon topoisomerase (DNA) II alpha 170kDa (TOP2A), mRNA.

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CloneID	OPh121096
Clone ID Related Accession (Same CDS sequence)	XM_007112498.1
Accession Version	XM_007112498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	DNA topoisomerase 2-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006714299.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CGCCACTGCA GCCTGTAAAT GACAATATGC AAGTCAACAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGAATCT ATCAAAAGAA GACACAACTG 
GAACATATTC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TATTGGCATT AACTGTAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGAGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAGAATA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAGA 
GCTGGTGAGA TGGAACTCAG GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCGT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTTTCT CAATGGAAAT 
AAGCTTCCAA TAAAAGGATT CCGTAGCTAT GTAGATATGT ATCTGAAGGA TAAGGTAGAT 
GAAACTGGCA ATCCACTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTGTGT 
TTAACAATGA GTGAAAAAGG CTTTCAACAA ATCAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAC TGAGTGAAAA ATTCATCAAA 
GCTGCAACTG GCTGTGGTAT TGTAGAAAGC ATACTAAATT GGGTGAAATT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCCACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TACGGGGTTT TCCCCCTAAG AGGAAAAATG CTTAATGTTC GAGAAGCTTC TCATAAGCAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTGCGTTACG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAGT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACGCCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG CACCAGCACA 
TCAAAAGAAG CTAAAGAGTA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCAGGTCCTG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGGC AACGAAAGTT ACTTGGCCTT 
CCTGAGGATT ACTTATATGG GCAAACTACC ACGTATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAGAGGT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG TAGCAATAAT 
CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TGCATGGTGG CAAGGATTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCACCA 
AAAGATGATC ACACGTTAAA GTTTTTATAT GATGACAACC AACGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGCTGGTA AATGGCGCTG AAGGAATTGG TACTGGCTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAATATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATATAAAGA ACAGGTTCTA 
GAACCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTGA TGACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAG AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATACGACA CAGTGTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAGTGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATCGAAAA CAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAAAGA 
GGCTATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG GAAACTGAAA AGAGAGACTC TGTAACAGAT 
TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAT TTGGCTGCTT TTATTGAAGA GCTGGAGGCT 
GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAG GGCAGAAGTT TTGCCTTCTC CATGTGGAAA AAGAGTCATC 
CCCCAAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA AAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGGATGG AAATAGAAGG CTTAAAACAG 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAGACA AACTACATTG 
CCATTTAAAC CAGTCAAGAA AGCCAAAAAA AGAAACCCCT GGTCTGATTC AGAATCAGAT 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCCCGAG AAAAAGAGCC ACGGAGAGCA 
GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAAATTTCTC AGATTTTGAT 
GAAAACAGTC AAGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAG GACCAAAACT 
TCCCCAAAAC ATACTAACAA AGAACTGAAG CCACAGAAAA GTGCCACATC AGAAACGGAC 
CTTAATGATG ATGATGCCAA GGATAATGTA CCACTTCCTC CAAGCTCTCC TGCTGCTGAT 
TTCCCAGCTT TAACTAAAAC CATAAACCCT GTTCCTAAAA AGAATGTGAC AGTGAAGAAG 
ACAGCAGCAA AAAGTCAGTC TTCCACCTCT ACTACAGGGG CCAAAAAAGG GGCTGCACCA 
AAAAGAACCA AAAAGGATCC AGACTTGGAT TCTGATGTCT CTAAAAAGCC TAATCCCCCC 
AAAACCAAGA GTCGCCGCAA AAGGAAGCTG TCCACTTCTG ATGATTCTGA CTCTAATTTT 
GAGAAAATGA TTTCTAAAAC AGTCACAAGC AAGAAACCCA AGGAGGACAG CGATGACTTC 
CATCTGGACT TAGACTCAGC TGTGGCTTCT CGGGCAAAAT CTGGACGGAC AAAGAAACCT 
ATAAAGTACC TCGAAGAATC AGATGAAGAT GATCTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007112560.1
CDS	134..4735
Translation	MEVSPLQPVNDNMQVNKTKKNEDAKKRLSIERIYQKKTQLEHILLRPDTYIGSVESVTQQMWVYDEDIGINCREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELRPFSGEDYTCITFHPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPIKGFRSYVDMYLKDKVDETGNPLKIIHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAATGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKMLNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEVAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWSCKIPNFDVREVVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLMTDYREYHTDTTVKFVVKMTEEKLAEAERVGLHRVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKRDSVTDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFIEELEAVEAKEKQDEQIGLPGKGGKAKGKKTQRAEVLPSPCGKRVIPQVTIEMKAEAEKKIKKKIKNENTEGSPQEDGMEIEGLKQRLEKKQKREPGTKTKRQTTLPFKPVKKAKKRNPWSDSESDMSSNESNFDVPPREKEPRRAATKTKFTMDLDSDENFSDFDENSQDEDFVPSDASPPRTKTSPKHTNKELKPQKSATSETDLNDDDAKDNVPLPPSSPAADFPALTKTINPVPKKNVTVKKTAAKSQSSTSTTGAKKGAAPKRTKKDPDLDSDVSKKPNPPKTKSRRKRKLSTSDDSDSNFEKMISKTVTSKKPKEDSDDFHLDLDSAVASRAKSGRTKKPIKYLEESDEDDLF

Target ORF information:

RefSeq Version	XM_007112498.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon topoisomerase (DNA) II alpha 170kDa (TOP2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007112498.1

ORF Insert Sequence:

ATGGAGGTGT CGCCACTGCA GCCTGTAAAT GACAATATGC AAGTCAACAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGAATCT ATCAAAAGAA GACACAACTG 
GAACATATTC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TATTGGCATT AACTGTAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGAGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAGAATA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAGA 
GCTGGTGAGA TGGAACTCAG GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCGT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTTTCT CAATGGAAAT 
AAGCTTCCAA TAAAAGGATT CCGTAGCTAT GTAGATATGT ATCTGAAGGA TAAGGTAGAT 
GAAACTGGCA ATCCACTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTGTGT 
TTAACAATGA GTGAAAAAGG CTTTCAACAA ATCAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAC TGAGTGAAAA ATTCATCAAA 
GCTGCAACTG GCTGTGGTAT TGTAGAAAGC ATACTAAATT GGGTGAAATT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCCACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TACGGGGTTT TCCCCCTAAG AGGAAAAATG CTTAATGTTC GAGAAGCTTC TCATAAGCAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTGCGTTACG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAGT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACGCCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG CACCAGCACA 
TCAAAAGAAG CTAAAGAGTA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCAGGTCCTG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGGC AACGAAAGTT ACTTGGCCTT 
CCTGAGGATT ACTTATATGG GCAAACTACC ACGTATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAGAGGT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG TAGCAATAAT 
CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TGCATGGTGG CAAGGATTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCACCA 
AAAGATGATC ACACGTTAAA GTTTTTATAT GATGACAACC AACGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGCTGGTA AATGGCGCTG AAGGAATTGG TACTGGCTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAATATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATATAAAGA ACAGGTTCTA 
GAACCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTGA TGACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAG AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATACGACA CAGTGTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAGTGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATCGAAAA CAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAAAGA 
GGCTATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG GAAACTGAAA AGAGAGACTC TGTAACAGAT 
TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAT TTGGCTGCTT TTATTGAAGA GCTGGAGGCT 
GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAG GGCAGAAGTT TTGCCTTCTC CATGTGGAAA AAGAGTCATC 
CCCCAAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA AAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGGATGG AAATAGAAGG CTTAAAACAG 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAGACA AACTACATTG 
CCATTTAAAC CAGTCAAGAA AGCCAAAAAA AGAAACCCCT GGTCTGATTC AGAATCAGAT 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCCCGAG AAAAAGAGCC ACGGAGAGCA 
GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAAATTTCTC AGATTTTGAT 
GAAAACAGTC AAGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAG GACCAAAACT 
TCCCCAAAAC ATACTAACAA AGAACTGAAG CCACAGAAAA GTGCCACATC AGAAACGGAC 
CTTAATGATG ATGATGCCAA GGATAATGTA CCACTTCCTC CAAGCTCTCC TGCTGCTGAT 
TTCCCAGCTT TAACTAAAAC CATAAACCCT GTTCCTAAAA AGAATGTGAC AGTGAAGAAG 
ACAGCAGCAA AAAGTCAGTC TTCCACCTCT ACTACAGGGG CCAAAAAAGG GGCTGCACCA 
AAAAGAACCA AAAAGGATCC AGACTTGGAT TCTGATGTCT CTAAAAAGCC TAATCCCCCC 
AAAACCAAGA GTCGCCGCAA AAGGAAGCTG TCCACTTCTG ATGATTCTGA CTCTAATTTT 
GAGAAAATGA TTTCTAAAAC AGTCACAAGC AAGAAACCCA AGGAGGACAG CGATGACTTC 
CATCTGGACT TAGACTCAGC TGTGGCTTCT CGGGCAAAAT CTGGACGGAC AAAGAAACCT 
ATAAAGTACC TCGAAGAATC AGATGAAGAT GATCTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_007112498.3 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007112560.2 Physeter catodon DNA topoisomerase II alpha (TOP2A), mRNA.

Price & Availability↑

CloneID	OPh298989
Clone ID Related Accession (Same CDS sequence)	XM_007112498.3 , XM_007112498.2
Accession Version	XM_007112498.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	DNA topoisomerase 2-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041227.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2019 this sequence version replaced XM_007112498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CGCCACTGCA GCCTGTAAAT GACAATATGC AAGTCAACAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGAATCT ATCAAAAGAA GACACAACTG 
GAACATATTC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TATTGGCATT AACTGTAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGAGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAGAATA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAGA 
GCTGGTGAGA TGGAACTCAG GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCGT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTTTCT CAATGGAAAT 
AAGCTTCCAA TAAAAGGATT CCGTAGCTAT GTAGATATGT ATCTGAAGGA TAAGGTAGAT 
GAAACTGGCA ATCCACTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTGTGT 
TTAACAATGA GTGAAAAAGG CTTTCAACAA ATCAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAC TGAGTGAAAA ATTCATCAAA 
GCTGCAACTG GCTGTGGTAT TGTAGAAAGC ATACTAAATT GGGTGAAATT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCCACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TACGGGGTTT TCCCCCTAAG AGGAAAAATG CTTAATGTTC GAGAAGCTTC TCATAAGCAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTGCGTTACG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAGT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACGCCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG CACCAGCACA 
TCAAAAGAAG CTAAAGAGTA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCAGGTCCTG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGGC AACGAAAGTT ACTTGGCCTT 
CCTGAGGATT ACTTATATGG GCAAACTACC ACGTATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAGAGGT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG TAGCAATAAT 
CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TGCATGGTGG CAAGGATTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCACCA 
AAAGATGATC ACACGTTAAA GTTTTTATAT GATGACAACC AACGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGCTGGTA AATGGCGCTG AAGGAATTGG TACTGGCTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAATATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATATAAAGA ACAGGTTCTA 
GAACCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTGA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAG AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATACGACA CAGTGTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAGTGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATCGAAAA CAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAAAGA 
GGCTATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG GAAACTGAAA AGAGAGACTC TGTAACAGAT 
TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAT TTGGCTGCTT TTATTGAAGA GCTGGAGGCT 
GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAG GGCAGAAGTT TTGCCTTCTC CATGTGGAAA AAGAGTCATC 
CCCCAAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA AAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGGATGG AAATAGAAGG CTTAAAACAG 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAGACA AACTACATTG 
CCATTTAAAC CAGTCAAGAA AGCCAAAAAA AGAAACCCCT GGTCTGATTC AGAATCAGAT 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCCCGAG AAAAAGAGCC ACGGAGAGCA 
GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAAATTTCTC AGATTTTGAT 
GAAAACAGTC AAGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAG GACCAAAACT 
TCCCCAAAAC ATACTAACAA AGAACTGAAG CCACAGAAAA GTGCCACATC AGAAACGGAC 
CTTAATGATG ATGATGCCAA GGATAATGTA CCACTTCCTC CAAGCTCTCC TGCTGCTGAT 
TTCCCAGCTT TAACTAAAAC CATAAACCCT GTTCCTAAAA AGAATGTGAC AGTGAAGAAG 
ACAGCAGCAA AAAGTCAGTC TTCCACCTCT ACTACAGGGG CCAAAAAAGG GGCTGCACCA 
AAAAGAACCA AAAAGGATCC AGACTTGGAT TCTGATGTCT CTAAAAAGCC TAATCCCCCC 
AAAACCAAGA GTCGCCGCAA AAGGAAGCTG TCCACTTCTG ATGATTCTGA CTCTAATTTT 
GAGAAAATGA TTTCTAAAAC AGTCACAAGC AAGAAACCCA AGGAGGACAG CGATGACTTC 
CATCTGGACT TAGACTCAGC TGTGGCTTCT CGGGCAAAAT CTGGACGGAC AAAGAAACCT 
ATAAAGTACC TCGAAGAATC AGATGAAGAT GATCTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007112560.2
CDS	136..4737
Translation	MEVSPLQPVNDNMQVNKTKKNEDAKKRLSIERIYQKKTQLEHILLRPDTYIGSVESVTQQMWVYDEDIGINCREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELRPFSGEDYTCITFHPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPIKGFRSYVDMYLKDKVDETGNPLKIIHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAATGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKMLNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEVAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWSCKIPNFDVREVVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHRVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKRDSVTDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFIEELEAVEAKEKQDEQIGLPGKGGKAKGKKTQRAEVLPSPCGKRVIPQVTIEMKAEAEKKIKKKIKNENTEGSPQEDGMEIEGLKQRLEKKQKREPGTKTKRQTTLPFKPVKKAKKRNPWSDSESDMSSNESNFDVPPREKEPRRAATKTKFTMDLDSDENFSDFDENSQDEDFVPSDASPPRTKTSPKHTNKELKPQKSATSETDLNDDDAKDNVPLPPSSPAADFPALTKTINPVPKKNVTVKKTAAKSQSSTSTTGAKKGAAPKRTKKDPDLDSDVSKKPNPPKTKSRRKRKLSTSDDSDSNFEKMISKTVTSKKPKEDSDDFHLDLDSAVASRAKSGRTKKPIKYLEESDEDDLF

Target ORF information:

RefSeq Version	XM_007112498.3
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon DNA topoisomerase II alpha (TOP2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007112498.3

ORF Insert Sequence:

ATGGAGGTGT CGCCACTGCA GCCTGTAAAT GACAATATGC AAGTCAACAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGAATCT ATCAAAAGAA GACACAACTG 
GAACATATTC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TATTGGCATT AACTGTAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGAGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAGAATA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAGA 
GCTGGTGAGA TGGAACTCAG GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCGT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTTTCT CAATGGAAAT 
AAGCTTCCAA TAAAAGGATT CCGTAGCTAT GTAGATATGT ATCTGAAGGA TAAGGTAGAT 
GAAACTGGCA ATCCACTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTGTGT 
TTAACAATGA GTGAAAAAGG CTTTCAACAA ATCAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAC TGAGTGAAAA ATTCATCAAA 
GCTGCAACTG GCTGTGGTAT TGTAGAAAGC ATACTAAATT GGGTGAAATT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCCACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TACGGGGTTT TCCCCCTAAG AGGAAAAATG CTTAATGTTC GAGAAGCTTC TCATAAGCAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTGCGTTACG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAGT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACGCCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG CACCAGCACA 
TCAAAAGAAG CTAAAGAGTA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCAGGTCCTG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGGC AACGAAAGTT ACTTGGCCTT 
CCTGAGGATT ACTTATATGG GCAAACTACC ACGTATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAGAGGT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG TAGCAATAAT 
CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TGCATGGTGG CAAGGATTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCACCA 
AAAGATGATC ACACGTTAAA GTTTTTATAT GATGACAACC AACGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGCTGGTA AATGGCGCTG AAGGAATTGG TACTGGCTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAATATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATATAAAGA ACAGGTTCTA 
GAACCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTGA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAG AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATACGACA CAGTGTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAGTGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATCGAAAA CAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAAAGA 
GGCTATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG GAAACTGAAA AGAGAGACTC TGTAACAGAT 
TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAT TTGGCTGCTT TTATTGAAGA GCTGGAGGCT 
GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAG GGCAGAAGTT TTGCCTTCTC CATGTGGAAA AAGAGTCATC 
CCCCAAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA AAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGGATGG AAATAGAAGG CTTAAAACAG 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAGACA AACTACATTG 
CCATTTAAAC CAGTCAAGAA AGCCAAAAAA AGAAACCCCT GGTCTGATTC AGAATCAGAT 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCCCGAG AAAAAGAGCC ACGGAGAGCA 
GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAAATTTCTC AGATTTTGAT 
GAAAACAGTC AAGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAG GACCAAAACT 
TCCCCAAAAC ATACTAACAA AGAACTGAAG CCACAGAAAA GTGCCACATC AGAAACGGAC 
CTTAATGATG ATGATGCCAA GGATAATGTA CCACTTCCTC CAAGCTCTCC TGCTGCTGAT 
TTCCCAGCTT TAACTAAAAC CATAAACCCT GTTCCTAAAA AGAATGTGAC AGTGAAGAAG 
ACAGCAGCAA AAAGTCAGTC TTCCACCTCT ACTACAGGGG CCAAAAAAGG GGCTGCACCA 
AAAAGAACCA AAAAGGATCC AGACTTGGAT TCTGATGTCT CTAAAAAGCC TAATCCCCCC 
AAAACCAAGA GTCGCCGCAA AAGGAAGCTG TCCACTTCTG ATGATTCTGA CTCTAATTTT 
GAGAAAATGA TTTCTAAAAC AGTCACAAGC AAGAAACCCA AGGAGGACAG CGATGACTTC 
CATCTGGACT TAGACTCAGC TGTGGCTTCT CGGGCAAAAT CTGGACGGAC AAAGAAACCT 
ATAAAGTACC TCGAAGAATC AGATGAAGAT GATCTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_007112498.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007112560.2 Physeter catodon DNA topoisomerase II alpha (TOP2A), mRNA.

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CloneID	OPh298989
Clone ID Related Accession (Same CDS sequence)	XM_007112498.3 , XM_007112498.2
Accession Version	XM_007112498.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	DNA topoisomerase 2-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873583.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 23, 2018 this sequence version replaced XM_007112498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CGCCACTGCA GCCTGTAAAT GACAATATGC AAGTCAACAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGAATCT ATCAAAAGAA GACACAACTG 
GAACATATTC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TATTGGCATT AACTGTAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGAGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAGAATA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAGA 
GCTGGTGAGA TGGAACTCAG GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCGT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTTTCT CAATGGAAAT 
AAGCTTCCAA TAAAAGGATT CCGTAGCTAT GTAGATATGT ATCTGAAGGA TAAGGTAGAT 
GAAACTGGCA ATCCACTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTGTGT 
TTAACAATGA GTGAAAAAGG CTTTCAACAA ATCAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAC TGAGTGAAAA ATTCATCAAA 
GCTGCAACTG GCTGTGGTAT TGTAGAAAGC ATACTAAATT GGGTGAAATT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCCACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TACGGGGTTT TCCCCCTAAG AGGAAAAATG CTTAATGTTC GAGAAGCTTC TCATAAGCAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTGCGTTACG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAGT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACGCCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG CACCAGCACA 
TCAAAAGAAG CTAAAGAGTA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCAGGTCCTG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGGC AACGAAAGTT ACTTGGCCTT 
CCTGAGGATT ACTTATATGG GCAAACTACC ACGTATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAGAGGT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG TAGCAATAAT 
CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TGCATGGTGG CAAGGATTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCACCA 
AAAGATGATC ACACGTTAAA GTTTTTATAT GATGACAACC AACGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGCTGGTA AATGGCGCTG AAGGAATTGG TACTGGCTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAATATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATATAAAGA ACAGGTTCTA 
GAACCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTGA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAG AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATACGACA CAGTGTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAGTGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATCGAAAA CAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAAAGA 
GGCTATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG GAAACTGAAA AGAGAGACTC TGTAACAGAT 
TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAT TTGGCTGCTT TTATTGAAGA GCTGGAGGCT 
GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAG GGCAGAAGTT TTGCCTTCTC CATGTGGAAA AAGAGTCATC 
CCCCAAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA AAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGGATGG AAATAGAAGG CTTAAAACAG 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAGACA AACTACATTG 
CCATTTAAAC CAGTCAAGAA AGCCAAAAAA AGAAACCCCT GGTCTGATTC AGAATCAGAT 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCCCGAG AAAAAGAGCC ACGGAGAGCA 
GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAAATTTCTC AGATTTTGAT 
GAAAACAGTC AAGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAG GACCAAAACT 
TCCCCAAAAC ATACTAACAA AGAACTGAAG CCACAGAAAA GTGCCACATC AGAAACGGAC 
CTTAATGATG ATGATGCCAA GGATAATGTA CCACTTCCTC CAAGCTCTCC TGCTGCTGAT 
TTCCCAGCTT TAACTAAAAC CATAAACCCT GTTCCTAAAA AGAATGTGAC AGTGAAGAAG 
ACAGCAGCAA AAAGTCAGTC TTCCACCTCT ACTACAGGGG CCAAAAAAGG GGCTGCACCA 
AAAAGAACCA AAAAGGATCC AGACTTGGAT TCTGATGTCT CTAAAAAGCC TAATCCCCCC 
AAAACCAAGA GTCGCCGCAA AAGGAAGCTG TCCACTTCTG ATGATTCTGA CTCTAATTTT 
GAGAAAATGA TTTCTAAAAC AGTCACAAGC AAGAAACCCA AGGAGGACAG CGATGACTTC 
CATCTGGACT TAGACTCAGC TGTGGCTTCT CGGGCAAAAT CTGGACGGAC AAAGAAACCT 
ATAAAGTACC TCGAAGAATC AGATGAAGAT GATCTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007112560.2
CDS	135..4736
Translation	MEVSPLQPVNDNMQVNKTKKNEDAKKRLSIERIYQKKTQLEHILLRPDTYIGSVESVTQQMWVYDEDIGINCREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELRPFSGEDYTCITFHPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPIKGFRSYVDMYLKDKVDETGNPLKIIHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAATGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKMLNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEVAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWSCKIPNFDVREVVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHRVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKRDSVTDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFIEELEAVEAKEKQDEQIGLPGKGGKAKGKKTQRAEVLPSPCGKRVIPQVTIEMKAEAEKKIKKKIKNENTEGSPQEDGMEIEGLKQRLEKKQKREPGTKTKRQTTLPFKPVKKAKKRNPWSDSESDMSSNESNFDVPPREKEPRRAATKTKFTMDLDSDENFSDFDENSQDEDFVPSDASPPRTKTSPKHTNKELKPQKSATSETDLNDDDAKDNVPLPPSSPAADFPALTKTINPVPKKNVTVKKTAAKSQSSTSTTGAKKGAAPKRTKKDPDLDSDVSKKPNPPKTKSRRKRKLSTSDDSDSNFEKMISKTVTSKKPKEDSDDFHLDLDSAVASRAKSGRTKKPIKYLEESDEDDLF

Target ORF information:

RefSeq Version	XM_007112498.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon DNA topoisomerase II alpha (TOP2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007112498.2

ORF Insert Sequence:

ATGGAGGTGT CGCCACTGCA GCCTGTAAAT GACAATATGC AAGTCAACAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGAATCT ATCAAAAGAA GACACAACTG 
GAACATATTC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TATTGGCATT AACTGTAGGG AAGTCACTTT TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGAGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAGAATA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAATTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAGA 
GCTGGTGAGA TGGAACTCAG GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCGT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTTTCT CAATGGAAAT 
AAGCTTCCAA TAAAAGGATT CCGTAGCTAT GTAGATATGT ATCTGAAGGA TAAGGTAGAT 
GAAACTGGCA ATCCACTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTGTGT 
TTAACAATGA GTGAAAAAGG CTTTCAACAA ATCAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAC TGAGTGAAAA ATTCATCAAA 
GCTGCAACTG GCTGTGGTAT TGTAGAAAGC ATACTAAATT GGGTGAAATT TAAGGCCCAA 
GTCCAGTTAA ACAAGAAGTG TTCAGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCCACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TACGGGGTTT TCCCCCTAAG AGGAAAAATG CTTAATGTTC GAGAAGCTTC TCATAAGCAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTGCGTTACG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAGT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACGCCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG CACCAGCACA 
TCAAAAGAAG CTAAAGAGTA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCAGGTCCTG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGGC AACGAAAGTT ACTTGGCCTT 
CCTGAGGATT ACTTATATGG GCAAACTACC ACGTATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAGAGGT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG TAGCAATAAT 
CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACCAGGC TGCATGGTGG CAAGGATTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCACCA 
AAAGATGATC ACACGTTAAA GTTTTTATAT GATGACAACC AACGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGCTGGTA AATGGCGCTG AAGGAATTGG TACTGGCTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAATATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAA 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACA TGGACCCAGA CATATAAAGA ACAGGTTCTA 
GAACCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTGA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAG AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG 
GTGCTTTTTG ACCACGTAGG CTGTTTAAAG AAATACGACA CAGTGTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAGTGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATCGAAAA CAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAAAGA 
GGCTATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG GAAACTGAAA AGAGAGACTC TGTAACAGAT 
TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAT TTGGCTGCTT TTATTGAAGA GCTGGAGGCT 
GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAG GGCAGAAGTT TTGCCTTCTC CATGTGGAAA AAGAGTCATC 
CCCCAAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA AAAAATTAAG 
AATGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGGATGG AAATAGAAGG CTTAAAACAG 
AGATTAGAAA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAGACA AACTACATTG 
CCATTTAAAC CAGTCAAGAA AGCCAAAAAA AGAAACCCCT GGTCTGATTC AGAATCAGAT 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCCCGAG AAAAAGAGCC ACGGAGAGCA 
GCAACAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAAATTTCTC AGATTTTGAT 
GAAAACAGTC AAGATGAAGA TTTTGTCCCA TCAGATGCTA GTCCACCTAG GACCAAAACT 
TCCCCAAAAC ATACTAACAA AGAACTGAAG CCACAGAAAA GTGCCACATC AGAAACGGAC 
CTTAATGATG ATGATGCCAA GGATAATGTA CCACTTCCTC CAAGCTCTCC TGCTGCTGAT 
TTCCCAGCTT TAACTAAAAC CATAAACCCT GTTCCTAAAA AGAATGTGAC AGTGAAGAAG 
ACAGCAGCAA AAAGTCAGTC TTCCACCTCT ACTACAGGGG CCAAAAAAGG GGCTGCACCA 
AAAAGAACCA AAAAGGATCC AGACTTGGAT TCTGATGTCT CTAAAAAGCC TAATCCCCCC 
AAAACCAAGA GTCGCCGCAA AAGGAAGCTG TCCACTTCTG ATGATTCTGA CTCTAATTTT 
GAGAAAATGA TTTCTAAAAC AGTCACAAGC AAGAAACCCA AGGAGGACAG CGATGACTTC 
CATCTGGACT TAGACTCAGC TGTGGCTTCT CGGGCAAAAT CTGGACGGAC AAAGAAACCT 
ATAAAGTACC TCGAAGAATC AGATGAAGAT GATCTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TOP2A cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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