HIF1A cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIF1A
Entrez Gene ID	102978574
Full Name	hypoxia inducible factor 1 alpha subunit
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007109894.1	XP_007109956.1	hypoxia-inducible factor 1-alpha isoform X2
XM_007109893.3	XP_007109955.2	hypoxia-inducible factor 1-alpha isoform X1
XM_007109893.1	XP_007109955.1	hypoxia-inducible factor 1-alpha isoform X1
XM_007109894.2	XP_007109956.2	hypoxia-inducible factor 1-alpha isoform X2
XM_007109894.3	XP_007109956.2	hypoxia-inducible factor 1-alpha isoform X2
XM_007109893.2	XP_007109955.2	hypoxia-inducible factor 1-alpha isoform X1

Pathways from BioSystems
Gene Ontology

The following HIF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh118896	XM_007109893.1 Latest version!	Physeter catodon hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OPh118897	XM_007109894.1 Latest version!	Physeter catodon hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OPh286346	XM_007109893.3 Latest version!	Physeter catodon hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OPh286347	XM_007109894.3 Latest version!	Physeter catodon hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OPh286347	XM_007109894.2	Physeter catodon hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OPh286346	XM_007109893.2	Physeter catodon hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIF1A ( XM_007109893.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007109955.1 Physeter catodon hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh118896
Clone ID Related Accession (Same CDS sequence)	XM_007109893.1
Accession Version	XM_007109893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	hypoxia-inducible factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006713821.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGACGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCATAATGTG AGCTCACATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACCGGACAC AGTGTATTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TATGATACCA ACAGTAACCA ATCTCAATGT GGTTATAAGA AACCACCTAT GACGTGCCTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAGTTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGCT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAACCACAGT GCATTGTATG TGTAAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGTCTCTT TGATAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAAACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAATGATG TAATGCTCCC CTCATCCAAT 
GAGAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AATCAGGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTCACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGCCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGACCAGTTG 
TCACCATTGG AAAGCAGTTC TACTACTCAA AGTGCGAGCA CAATTACAGT ATTCCAGCCG 
ACTCAAATGC AGGAACCTCC TATAACCACC AACACTACCA CAGCCACCAC TGATGAATTA 
AAAACAGTGA CAAAAGATGG TATGGAAGAC ATTAAAATAT TGATTGCATT TCCATCTCCT 
CCCCACGTAC CTAAAGAAAC TACTTGTGCC ACAACATCAC CATATAGTGA CACTGGAAGT 
CGGACAGCCT CACCAAACAG AGCAGGAAAA GGAGTCATAG AACAGACAGA AAAATCTCAT 
CCAAGAAGCC CTAATGTGTT ATCTGTCGCT TTGAGTCAAA GAACTACAGT TCCTGAGGAA 
GAACTAAGTC CAAAGATACT AGCTTTGCAG AATGCTCAGA GAAAGCGAAA AATAGAACAT 
GATGGTTCAC TTTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAACC AGATGATCGT 
GCAACTACTA CATCACTTTC TTGGAAACGC GTAAAAGGAT GCAAATCTAG TGAACAGAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007109955.1
CDS	301..2769
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSTTQSASTITVFQPTQMQEPPITTNTTTATTDELKTVTKDGMEDIKILIAFPSPPHVPKETTCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELSPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_007109893.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007109893.1

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGACGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCATAATGTG AGCTCACATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACCGGACAC AGTGTATTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TATGATACCA ACAGTAACCA ATCTCAATGT GGTTATAAGA AACCACCTAT GACGTGCCTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAGTTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGCT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAACCACAGT GCATTGTATG TGTAAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGTCTCTT TGATAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAAACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAATGATG TAATGCTCCC CTCATCCAAT 
GAGAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AATCAGGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTCACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGCCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGACCAGTTG 
TCACCATTGG AAAGCAGTTC TACTACTCAA AGTGCGAGCA CAATTACAGT ATTCCAGCCG 
ACTCAAATGC AGGAACCTCC TATAACCACC AACACTACCA CAGCCACCAC TGATGAATTA 
AAAACAGTGA CAAAAGATGG TATGGAAGAC ATTAAAATAT TGATTGCATT TCCATCTCCT 
CCCCACGTAC CTAAAGAAAC TACTTGTGCC ACAACATCAC CATATAGTGA CACTGGAAGT 
CGGACAGCCT CACCAAACAG AGCAGGAAAA GGAGTCATAG AACAGACAGA AAAATCTCAT 
CCAAGAAGCC CTAATGTGTT ATCTGTCGCT TTGAGTCAAA GAACTACAGT TCCTGAGGAA 
GAACTAAGTC CAAAGATACT AGCTTTGCAG AATGCTCAGA GAAAGCGAAA AATAGAACAT 
GATGGTTCAC TTTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAACC AGATGATCGT 
GCAACTACTA CATCACTTTC TTGGAAACGC GTAAAAGGAT GCAAATCTAG TGAACAGAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_007109894.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007109956.1 Physeter catodon hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPh118897
Clone ID Related Accession (Same CDS sequence)	XM_007109894.1
Accession Version	XM_007109894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	hypoxia-inducible factor 1-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006713821.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTA CCATCAGCTA TTTGCGTGTG AGGAAACTTC TGGATGCTGG TGATTTGGAT 
ATTGAAGATG AAATGAAGGC ACAGATGAAT TGCTTTTATT TGAAAGCCTT GGATGGTTTT 
GTTATGGTTC TCACAGATGA TGGTGACATG ATTTACATTT CTGATAATGT GAACAAATAC 
ATGGGATTAA CTCAGTTTGA ACTAACCGGA CACAGTGTAT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCTTGTGAA AAAGGGTAAA 
GAGCAAAATA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GTACCCTAAC TAGCCGGGGG 
AGAACTATGA ACATAAAGTC TGCAACATGG AAAGTACTTC ACTGCACAGG CCATATTCAT 
GTATATGATA CCAACAGTAA CCAATCTCAA TGTGGTTATA AGAAACCACC TATGACGTGC 
CTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAATA TTGAAATTCC TTTAGACAGC 
AAGACTTTTC TCAGTCGTCA CAGTCTGGAT ATGAAGTTTT CTTATTGTGA TGAAAGAATT 
ACAGAATTGA TGGGATATGA GCCAGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ACTCTGATCA TCTGACCAAA ACTCATCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GCTATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA CACTAAGAAC TCTCAACCAC AGTGCATTGT ATGTGTAAAT 
TATGTTGTGA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATGT 
GTCCTCAAAC CAGTTGAATC TTCAGACATG AAAATGACTC AGCTATTCAC CAAAGTTGAA 
TCAGAAGATA CAAGTAGTCT CTTTGATAAA CTTAAGAAGG AACCTGATGC TTTAACTTTG 
CTGGCCCCAG CTGCTGGAGA CACAATCATA TCTTTAGATT TTGGCAGCAA TGACACAGAA 
ACTGATGACC AACAACTTGA GGAAGTTCCA TTGTATAATG ATGTAATGCT CCCCTCATCC 
AATGAGAAAT TACAGAATAT AAATTTGGCA ATGTCTCCAT TACCTGCCTC TGAAACTCCA 
AAGCCACTTA GAAGTAGTGC TGACCCTGCA CTCAATCAGG AAGTTGCATT AAAGTTAGAA 
CCAAATCCAG AGTCACTGGA ACTTTCTTTC ACCATGCCCC AGATTCAAGA TCAGCCAGCT 
AGTCCTTCTG ATGGAAGCAC CAGACAAAGT TCACCTGAGC CTAACAGCCC CAGTGAATAT 
TGTTTTGATG TGGATAGTGA TATGGTCAAT GAATTCAAGT TGGAATTGGT AGAGAAACTT 
TTTGCTGAAG ACACAGAAGC AAAGAATCCA TTTTCCACTC AGGACACTGA TTTAGACTTG 
GAGATGTTAG CTCCTTATAT CCCAATGGAT GATGACTTCC AGTTACGTTC CTTCGACCAG 
TTGTCACCAT TGGAAAGCAG TTCTACTACT CAAAGTGCGA GCACAATTAC AGTATTCCAG 
CCGACTCAAA TGCAGGAACC TCCTATAACC ACCAACACTA CCACAGCCAC CACTGATGAA 
TTAAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TATTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA AACTACTTGT GCCACAACAT CACCATATAG TGACACTGGA 
AGTCGGACAG CCTCACCAAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC AGTTCCTGAG 
GAAGAACTAA GTCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATAGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CTACATCACT TTCTTGGAAA CGCGTAAAAG GATGCAAATC TAGTGAACAG 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007109956.1
CDS	556..2847
Translation	MRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSTTQSASTITVFQPTQMQEPPITTNTTTATTDELKTVTKDGMEDIKILIAFPSPPHVPKETTCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELSPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_007109894.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007109894.1

ORF Insert Sequence:

ATGAGGCTTA CCATCAGCTA TTTGCGTGTG AGGAAACTTC TGGATGCTGG TGATTTGGAT 
ATTGAAGATG AAATGAAGGC ACAGATGAAT TGCTTTTATT TGAAAGCCTT GGATGGTTTT 
GTTATGGTTC TCACAGATGA TGGTGACATG ATTTACATTT CTGATAATGT GAACAAATAC 
ATGGGATTAA CTCAGTTTGA ACTAACCGGA CACAGTGTAT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCTTGTGAA AAAGGGTAAA 
GAGCAAAATA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GTACCCTAAC TAGCCGGGGG 
AGAACTATGA ACATAAAGTC TGCAACATGG AAAGTACTTC ACTGCACAGG CCATATTCAT 
GTATATGATA CCAACAGTAA CCAATCTCAA TGTGGTTATA AGAAACCACC TATGACGTGC 
CTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAATA TTGAAATTCC TTTAGACAGC 
AAGACTTTTC TCAGTCGTCA CAGTCTGGAT ATGAAGTTTT CTTATTGTGA TGAAAGAATT 
ACAGAATTGA TGGGATATGA GCCAGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ACTCTGATCA TCTGACCAAA ACTCATCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GCTATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA CACTAAGAAC TCTCAACCAC AGTGCATTGT ATGTGTAAAT 
TATGTTGTGA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATGT 
GTCCTCAAAC CAGTTGAATC TTCAGACATG AAAATGACTC AGCTATTCAC CAAAGTTGAA 
TCAGAAGATA CAAGTAGTCT CTTTGATAAA CTTAAGAAGG AACCTGATGC TTTAACTTTG 
CTGGCCCCAG CTGCTGGAGA CACAATCATA TCTTTAGATT TTGGCAGCAA TGACACAGAA 
ACTGATGACC AACAACTTGA GGAAGTTCCA TTGTATAATG ATGTAATGCT CCCCTCATCC 
AATGAGAAAT TACAGAATAT AAATTTGGCA ATGTCTCCAT TACCTGCCTC TGAAACTCCA 
AAGCCACTTA GAAGTAGTGC TGACCCTGCA CTCAATCAGG AAGTTGCATT AAAGTTAGAA 
CCAAATCCAG AGTCACTGGA ACTTTCTTTC ACCATGCCCC AGATTCAAGA TCAGCCAGCT 
AGTCCTTCTG ATGGAAGCAC CAGACAAAGT TCACCTGAGC CTAACAGCCC CAGTGAATAT 
TGTTTTGATG TGGATAGTGA TATGGTCAAT GAATTCAAGT TGGAATTGGT AGAGAAACTT 
TTTGCTGAAG ACACAGAAGC AAAGAATCCA TTTTCCACTC AGGACACTGA TTTAGACTTG 
GAGATGTTAG CTCCTTATAT CCCAATGGAT GATGACTTCC AGTTACGTTC CTTCGACCAG 
TTGTCACCAT TGGAAAGCAG TTCTACTACT CAAAGTGCGA GCACAATTAC AGTATTCCAG 
CCGACTCAAA TGCAGGAACC TCCTATAACC ACCAACACTA CCACAGCCAC CACTGATGAA 
TTAAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TATTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA AACTACTTGT GCCACAACAT CACCATATAG TGACACTGGA 
AGTCGGACAG CCTCACCAAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC AGTTCCTGAG 
GAAGAACTAA GTCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATAGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CTACATCACT TTCTTGGAAA CGCGTAAAAG GATGCAAATC TAGTGAACAG 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_007109893.3 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007109955.2 Physeter catodon hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh286346
Clone ID Related Accession (Same CDS sequence)	XM_007109893.3 , XM_007109893.2
Accession Version	XM_007109893.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	hypoxia-inducible factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041224.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2019 this sequence version replaced XM_007109893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGACGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCATAATGTG AGCTCACATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACCGGACAC AGTGTATTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TATGATACCA ACAGTAACCA ATCTCAATGT GGTTATAAGA AACCACCTAT GACGTGCCTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAGTTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGCT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAACCACAGT GCATTGTATG TGTAAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGTCTCTT TGATAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAAACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAATGATG TAATGCTCCC CTCATCCAAT 
GAGAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AATCAGGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTCACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGCCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGACCAGTTG 
TCACCATTGG AAAGCAGTTC TACTACTCAA AGTGCGAGCA CAATTACAGT ATTCCAGCCG 
ACTCAAATGC AGGAACCTCC TATAACCACC AACGCTACCA CAGCCACCAC TGATGAATTA 
AAAACAGTGA CAAAAGATGG TATGGAAGAC ATTAAAATAT TGATTGCATT TCCATCTCCT 
CCCCACGTAC CTAAAGAAAC TACTTGTGCC ACAACATCAC CATATAGTGA CACTGGAAGT 
CGGACAGCCT CACCAAACAG AGCAGGAAAA GGAGTCATAG AACAGACAGA AAAATCTCAT 
CCAAGAAGCC CTAATGTGTT ATCTGTCGCT TTGAGTCAAA GAACTACAGT TCCTGAGGAA 
GAACTAAGTC CAAAGATACT AGCTTTGCAG AATGCTCAGA GAAAGCGAAA AATAGAACAT 
GATGGTTCAC TTTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAACC AGATGATCGT 
GCAACTACTA CATCACTTTC TTGGAAACGC GTAAAAGGAT GCAAATCTAG TGAACAGAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007109955.2
CDS	302..2770
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSTTQSASTITVFQPTQMQEPPITTNATTATTDELKTVTKDGMEDIKILIAFPSPPHVPKETTCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELSPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_007109893.3
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007109893.3

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGACGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCATAATGTG AGCTCACATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACCGGACAC AGTGTATTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TATGATACCA ACAGTAACCA ATCTCAATGT GGTTATAAGA AACCACCTAT GACGTGCCTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAGTTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGCT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAACCACAGT GCATTGTATG TGTAAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGTCTCTT TGATAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAAACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAATGATG TAATGCTCCC CTCATCCAAT 
GAGAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AATCAGGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTCACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGCCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGACCAGTTG 
TCACCATTGG AAAGCAGTTC TACTACTCAA AGTGCGAGCA CAATTACAGT ATTCCAGCCG 
ACTCAAATGC AGGAACCTCC TATAACCACC AACGCTACCA CAGCCACCAC TGATGAATTA 
AAAACAGTGA CAAAAGATGG TATGGAAGAC ATTAAAATAT TGATTGCATT TCCATCTCCT 
CCCCACGTAC CTAAAGAAAC TACTTGTGCC ACAACATCAC CATATAGTGA CACTGGAAGT 
CGGACAGCCT CACCAAACAG AGCAGGAAAA GGAGTCATAG AACAGACAGA AAAATCTCAT 
CCAAGAAGCC CTAATGTGTT ATCTGTCGCT TTGAGTCAAA GAACTACAGT TCCTGAGGAA 
GAACTAAGTC CAAAGATACT AGCTTTGCAG AATGCTCAGA GAAAGCGAAA AATAGAACAT 
GATGGTTCAC TTTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAACC AGATGATCGT 
GCAACTACTA CATCACTTTC TTGGAAACGC GTAAAAGGAT GCAAATCTAG TGAACAGAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_007109893.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007109955.2 Physeter catodon hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh286346
Clone ID Related Accession (Same CDS sequence)	XM_007109893.3 , XM_007109893.2
Accession Version	XM_007109893.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	hypoxia-inducible factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873550.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 23, 2018 this sequence version replaced XM_007109893.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGACGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCATAATGTG AGCTCACATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACCGGACAC AGTGTATTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TATGATACCA ACAGTAACCA ATCTCAATGT GGTTATAAGA AACCACCTAT GACGTGCCTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAGTTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGCT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAACCACAGT GCATTGTATG TGTAAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGTCTCTT TGATAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAAACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAATGATG TAATGCTCCC CTCATCCAAT 
GAGAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AATCAGGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTCACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGCCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGACCAGTTG 
TCACCATTGG AAAGCAGTTC TACTACTCAA AGTGCGAGCA CAATTACAGT ATTCCAGCCG 
ACTCAAATGC AGGAACCTCC TATAACCACC AACGCTACCA CAGCCACCAC TGATGAATTA 
AAAACAGTGA CAAAAGATGG TATGGAAGAC ATTAAAATAT TGATTGCATT TCCATCTCCT 
CCCCACGTAC CTAAAGAAAC TACTTGTGCC ACAACATCAC CATATAGTGA CACTGGAAGT 
CGGACAGCCT CACCAAACAG AGCAGGAAAA GGAGTCATAG AACAGACAGA AAAATCTCAT 
CCAAGAAGCC CTAATGTGTT ATCTGTCGCT TTGAGTCAAA GAACTACAGT TCCTGAGGAA 
GAACTAAGTC CAAAGATACT AGCTTTGCAG AATGCTCAGA GAAAGCGAAA AATAGAACAT 
GATGGTTCAC TTTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAACC AGATGATCGT 
GCAACTACTA CATCACTTTC TTGGAAACGC GTAAAAGGAT GCAAATCTAG TGAACAGAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007109955.2
CDS	302..2770
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSTTQSASTITVFQPTQMQEPPITTNATTATTDELKTVTKDGMEDIKILIAFPSPPHVPKETTCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELSPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_007109893.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007109893.2

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGACGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCATAATGTG AGCTCACATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACCGGACAC AGTGTATTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TATGATACCA ACAGTAACCA ATCTCAATGT GGTTATAAGA AACCACCTAT GACGTGCCTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAGTTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGCT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAACCACAGT GCATTGTATG TGTAAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGTCTCTT TGATAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAAACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAATGATG TAATGCTCCC CTCATCCAAT 
GAGAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AATCAGGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTCACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGCCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGACCAGTTG 
TCACCATTGG AAAGCAGTTC TACTACTCAA AGTGCGAGCA CAATTACAGT ATTCCAGCCG 
ACTCAAATGC AGGAACCTCC TATAACCACC AACGCTACCA CAGCCACCAC TGATGAATTA 
AAAACAGTGA CAAAAGATGG TATGGAAGAC ATTAAAATAT TGATTGCATT TCCATCTCCT 
CCCCACGTAC CTAAAGAAAC TACTTGTGCC ACAACATCAC CATATAGTGA CACTGGAAGT 
CGGACAGCCT CACCAAACAG AGCAGGAAAA GGAGTCATAG AACAGACAGA AAAATCTCAT 
CCAAGAAGCC CTAATGTGTT ATCTGTCGCT TTGAGTCAAA GAACTACAGT TCCTGAGGAA 
GAACTAAGTC CAAAGATACT AGCTTTGCAG AATGCTCAGA GAAAGCGAAA AATAGAACAT 
GATGGTTCAC TTTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAACC AGATGATCGT 
GCAACTACTA CATCACTTTC TTGGAAACGC GTAAAAGGAT GCAAATCTAG TGAACAGAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_007109894.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007109956.2 Physeter catodon hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPh286347
Clone ID Related Accession (Same CDS sequence)	XM_007109894.2 , XM_007109894.3
Accession Version	XM_007109894.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	hypoxia-inducible factor 1-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873550.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 23, 2018 this sequence version replaced XM_007109894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTA CCATCAGCTA TTTGCGTGTG AGGAAACTTC TGGATGCTGG TGATTTGGAT 
ATTGAAGATG AAATGAAGGC ACAGATGAAT TGCTTTTATT TGAAAGCCTT GGATGGTTTT 
GTTATGGTTC TCACAGATGA TGGTGACATG ATTTACATTT CTGATAATGT GAACAAATAC 
ATGGGATTAA CTCAGTTTGA ACTAACCGGA CACAGTGTAT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCTTGTGAA AAAGGGTAAA 
GAGCAAAATA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GTACCCTAAC TAGCCGGGGG 
AGAACTATGA ACATAAAGTC TGCAACATGG AAAGTACTTC ACTGCACAGG CCATATTCAT 
GTATATGATA CCAACAGTAA CCAATCTCAA TGTGGTTATA AGAAACCACC TATGACGTGC 
CTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAATA TTGAAATTCC TTTAGACAGC 
AAGACTTTTC TCAGTCGTCA CAGTCTGGAT ATGAAGTTTT CTTATTGTGA TGAAAGAATT 
ACAGAATTGA TGGGATATGA GCCAGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ACTCTGATCA TCTGACCAAA ACTCATCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GCTATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA CACTAAGAAC TCTCAACCAC AGTGCATTGT ATGTGTAAAT 
TATGTTGTGA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATGT 
GTCCTCAAAC CAGTTGAATC TTCAGACATG AAAATGACTC AGCTATTCAC CAAAGTTGAA 
TCAGAAGATA CAAGTAGTCT CTTTGATAAA CTTAAGAAGG AACCTGATGC TTTAACTTTG 
CTGGCCCCAG CTGCTGGAGA CACAATCATA TCTTTAGATT TTGGCAGCAA TGACACAGAA 
ACTGATGACC AACAACTTGA GGAAGTTCCA TTGTATAATG ATGTAATGCT CCCCTCATCC 
AATGAGAAAT TACAGAATAT AAATTTGGCA ATGTCTCCAT TACCTGCCTC TGAAACTCCA 
AAGCCACTTA GAAGTAGTGC TGACCCTGCA CTCAATCAGG AAGTTGCATT AAAGTTAGAA 
CCAAATCCAG AGTCACTGGA ACTTTCTTTC ACCATGCCCC AGATTCAAGA TCAGCCAGCT 
AGTCCTTCTG ATGGAAGCAC CAGACAAAGT TCACCTGAGC CTAACAGCCC CAGTGAATAT 
TGTTTTGATG TGGATAGTGA TATGGTCAAT GAATTCAAGT TGGAATTGGT AGAGAAACTT 
TTTGCTGAAG ACACAGAAGC AAAGAATCCA TTTTCCACTC AGGACACTGA TTTAGACTTG 
GAGATGTTAG CTCCTTATAT CCCAATGGAT GATGACTTCC AGTTACGTTC CTTCGACCAG 
TTGTCACCAT TGGAAAGCAG TTCTACTACT CAAAGTGCGA GCACAATTAC AGTATTCCAG 
CCGACTCAAA TGCAGGAACC TCCTATAACC ACCAACGCTA CCACAGCCAC CACTGATGAA 
TTAAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TATTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA AACTACTTGT GCCACAACAT CACCATATAG TGACACTGGA 
AGTCGGACAG CCTCACCAAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC AGTTCCTGAG 
GAAGAACTAA GTCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATAGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CTACATCACT TTCTTGGAAA CGCGTAAAAG GATGCAAATC TAGTGAACAG 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007109956.2
CDS	556..2847
Translation	MRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSTTQSASTITVFQPTQMQEPPITTNATTATTDELKTVTKDGMEDIKILIAFPSPPHVPKETTCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELSPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_007109894.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007109894.2

ORF Insert Sequence:

ATGAGGCTTA CCATCAGCTA TTTGCGTGTG AGGAAACTTC TGGATGCTGG TGATTTGGAT 
ATTGAAGATG AAATGAAGGC ACAGATGAAT TGCTTTTATT TGAAAGCCTT GGATGGTTTT 
GTTATGGTTC TCACAGATGA TGGTGACATG ATTTACATTT CTGATAATGT GAACAAATAC 
ATGGGATTAA CTCAGTTTGA ACTAACCGGA CACAGTGTAT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCTTGTGAA AAAGGGTAAA 
GAGCAAAATA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GTACCCTAAC TAGCCGGGGG 
AGAACTATGA ACATAAAGTC TGCAACATGG AAAGTACTTC ACTGCACAGG CCATATTCAT 
GTATATGATA CCAACAGTAA CCAATCTCAA TGTGGTTATA AGAAACCACC TATGACGTGC 
CTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAATA TTGAAATTCC TTTAGACAGC 
AAGACTTTTC TCAGTCGTCA CAGTCTGGAT ATGAAGTTTT CTTATTGTGA TGAAAGAATT 
ACAGAATTGA TGGGATATGA GCCAGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ACTCTGATCA TCTGACCAAA ACTCATCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GCTATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA CACTAAGAAC TCTCAACCAC AGTGCATTGT ATGTGTAAAT 
TATGTTGTGA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATGT 
GTCCTCAAAC CAGTTGAATC TTCAGACATG AAAATGACTC AGCTATTCAC CAAAGTTGAA 
TCAGAAGATA CAAGTAGTCT CTTTGATAAA CTTAAGAAGG AACCTGATGC TTTAACTTTG 
CTGGCCCCAG CTGCTGGAGA CACAATCATA TCTTTAGATT TTGGCAGCAA TGACACAGAA 
ACTGATGACC AACAACTTGA GGAAGTTCCA TTGTATAATG ATGTAATGCT CCCCTCATCC 
AATGAGAAAT TACAGAATAT AAATTTGGCA ATGTCTCCAT TACCTGCCTC TGAAACTCCA 
AAGCCACTTA GAAGTAGTGC TGACCCTGCA CTCAATCAGG AAGTTGCATT AAAGTTAGAA 
CCAAATCCAG AGTCACTGGA ACTTTCTTTC ACCATGCCCC AGATTCAAGA TCAGCCAGCT 
AGTCCTTCTG ATGGAAGCAC CAGACAAAGT TCACCTGAGC CTAACAGCCC CAGTGAATAT 
TGTTTTGATG TGGATAGTGA TATGGTCAAT GAATTCAAGT TGGAATTGGT AGAGAAACTT 
TTTGCTGAAG ACACAGAAGC AAAGAATCCA TTTTCCACTC AGGACACTGA TTTAGACTTG 
GAGATGTTAG CTCCTTATAT CCCAATGGAT GATGACTTCC AGTTACGTTC CTTCGACCAG 
TTGTCACCAT TGGAAAGCAG TTCTACTACT CAAAGTGCGA GCACAATTAC AGTATTCCAG 
CCGACTCAAA TGCAGGAACC TCCTATAACC ACCAACGCTA CCACAGCCAC CACTGATGAA 
TTAAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TATTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA AACTACTTGT GCCACAACAT CACCATATAG TGACACTGGA 
AGTCGGACAG CCTCACCAAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC AGTTCCTGAG 
GAAGAACTAA GTCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATAGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CTACATCACT TTCTTGGAAA CGCGTAAAAG GATGCAAATC TAGTGAACAG 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_007109894.3 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007109956.2 Physeter catodon hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X2, mRNA.

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CloneID	OPh286347
Clone ID Related Accession (Same CDS sequence)	XM_007109894.2 , XM_007109894.3
Accession Version	XM_007109894.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	hypoxia-inducible factor 1-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041224.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2019 this sequence version replaced XM_007109894.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTA CCATCAGCTA TTTGCGTGTG AGGAAACTTC TGGATGCTGG TGATTTGGAT 
ATTGAAGATG AAATGAAGGC ACAGATGAAT TGCTTTTATT TGAAAGCCTT GGATGGTTTT 
GTTATGGTTC TCACAGATGA TGGTGACATG ATTTACATTT CTGATAATGT GAACAAATAC 
ATGGGATTAA CTCAGTTTGA ACTAACCGGA CACAGTGTAT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCTTGTGAA AAAGGGTAAA 
GAGCAAAATA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GTACCCTAAC TAGCCGGGGG 
AGAACTATGA ACATAAAGTC TGCAACATGG AAAGTACTTC ACTGCACAGG CCATATTCAT 
GTATATGATA CCAACAGTAA CCAATCTCAA TGTGGTTATA AGAAACCACC TATGACGTGC 
CTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAATA TTGAAATTCC TTTAGACAGC 
AAGACTTTTC TCAGTCGTCA CAGTCTGGAT ATGAAGTTTT CTTATTGTGA TGAAAGAATT 
ACAGAATTGA TGGGATATGA GCCAGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ACTCTGATCA TCTGACCAAA ACTCATCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GCTATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA CACTAAGAAC TCTCAACCAC AGTGCATTGT ATGTGTAAAT 
TATGTTGTGA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATGT 
GTCCTCAAAC CAGTTGAATC TTCAGACATG AAAATGACTC AGCTATTCAC CAAAGTTGAA 
TCAGAAGATA CAAGTAGTCT CTTTGATAAA CTTAAGAAGG AACCTGATGC TTTAACTTTG 
CTGGCCCCAG CTGCTGGAGA CACAATCATA TCTTTAGATT TTGGCAGCAA TGACACAGAA 
ACTGATGACC AACAACTTGA GGAAGTTCCA TTGTATAATG ATGTAATGCT CCCCTCATCC 
AATGAGAAAT TACAGAATAT AAATTTGGCA ATGTCTCCAT TACCTGCCTC TGAAACTCCA 
AAGCCACTTA GAAGTAGTGC TGACCCTGCA CTCAATCAGG AAGTTGCATT AAAGTTAGAA 
CCAAATCCAG AGTCACTGGA ACTTTCTTTC ACCATGCCCC AGATTCAAGA TCAGCCAGCT 
AGTCCTTCTG ATGGAAGCAC CAGACAAAGT TCACCTGAGC CTAACAGCCC CAGTGAATAT 
TGTTTTGATG TGGATAGTGA TATGGTCAAT GAATTCAAGT TGGAATTGGT AGAGAAACTT 
TTTGCTGAAG ACACAGAAGC AAAGAATCCA TTTTCCACTC AGGACACTGA TTTAGACTTG 
GAGATGTTAG CTCCTTATAT CCCAATGGAT GATGACTTCC AGTTACGTTC CTTCGACCAG 
TTGTCACCAT TGGAAAGCAG TTCTACTACT CAAAGTGCGA GCACAATTAC AGTATTCCAG 
CCGACTCAAA TGCAGGAACC TCCTATAACC ACCAACGCTA CCACAGCCAC CACTGATGAA 
TTAAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TATTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA AACTACTTGT GCCACAACAT CACCATATAG TGACACTGGA 
AGTCGGACAG CCTCACCAAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC AGTTCCTGAG 
GAAGAACTAA GTCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATAGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CTACATCACT TTCTTGGAAA CGCGTAAAAG GATGCAAATC TAGTGAACAG 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007109956.2
CDS	557..2848
Translation	MRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSTTQSASTITVFQPTQMQEPPITTNATTATTDELKTVTKDGMEDIKILIAFPSPPHVPKETTCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELSPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_007109894.3
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007109894.3

ORF Insert Sequence:

ATGAGGCTTA CCATCAGCTA TTTGCGTGTG AGGAAACTTC TGGATGCTGG TGATTTGGAT 
ATTGAAGATG AAATGAAGGC ACAGATGAAT TGCTTTTATT TGAAAGCCTT GGATGGTTTT 
GTTATGGTTC TCACAGATGA TGGTGACATG ATTTACATTT CTGATAATGT GAACAAATAC 
ATGGGATTAA CTCAGTTTGA ACTAACCGGA CACAGTGTAT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCTTGTGAA AAAGGGTAAA 
GAGCAAAATA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GTACCCTAAC TAGCCGGGGG 
AGAACTATGA ACATAAAGTC TGCAACATGG AAAGTACTTC ACTGCACAGG CCATATTCAT 
GTATATGATA CCAACAGTAA CCAATCTCAA TGTGGTTATA AGAAACCACC TATGACGTGC 
CTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAATA TTGAAATTCC TTTAGACAGC 
AAGACTTTTC TCAGTCGTCA CAGTCTGGAT ATGAAGTTTT CTTATTGTGA TGAAAGAATT 
ACAGAATTGA TGGGATATGA GCCAGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ACTCTGATCA TCTGACCAAA ACTCATCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GCTATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA CACTAAGAAC TCTCAACCAC AGTGCATTGT ATGTGTAAAT 
TATGTTGTGA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATGT 
GTCCTCAAAC CAGTTGAATC TTCAGACATG AAAATGACTC AGCTATTCAC CAAAGTTGAA 
TCAGAAGATA CAAGTAGTCT CTTTGATAAA CTTAAGAAGG AACCTGATGC TTTAACTTTG 
CTGGCCCCAG CTGCTGGAGA CACAATCATA TCTTTAGATT TTGGCAGCAA TGACACAGAA 
ACTGATGACC AACAACTTGA GGAAGTTCCA TTGTATAATG ATGTAATGCT CCCCTCATCC 
AATGAGAAAT TACAGAATAT AAATTTGGCA ATGTCTCCAT TACCTGCCTC TGAAACTCCA 
AAGCCACTTA GAAGTAGTGC TGACCCTGCA CTCAATCAGG AAGTTGCATT AAAGTTAGAA 
CCAAATCCAG AGTCACTGGA ACTTTCTTTC ACCATGCCCC AGATTCAAGA TCAGCCAGCT 
AGTCCTTCTG ATGGAAGCAC CAGACAAAGT TCACCTGAGC CTAACAGCCC CAGTGAATAT 
TGTTTTGATG TGGATAGTGA TATGGTCAAT GAATTCAAGT TGGAATTGGT AGAGAAACTT 
TTTGCTGAAG ACACAGAAGC AAAGAATCCA TTTTCCACTC AGGACACTGA TTTAGACTTG 
GAGATGTTAG CTCCTTATAT CCCAATGGAT GATGACTTCC AGTTACGTTC CTTCGACCAG 
TTGTCACCAT TGGAAAGCAG TTCTACTACT CAAAGTGCGA GCACAATTAC AGTATTCCAG 
CCGACTCAAA TGCAGGAACC TCCTATAACC ACCAACGCTA CCACAGCCAC CACTGATGAA 
TTAAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TATTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA AACTACTTGT GCCACAACAT CACCATATAG TGACACTGGA 
AGTCGGACAG CCTCACCAAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC AGTTCCTGAG 
GAAGAACTAA GTCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATAGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CTACATCACT TTCTTGGAAA CGCGTAAAAG GATGCAAATC TAGTGAACAG 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HIF1A cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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