LOC100077465 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100077465
Entrez Gene ID	100077465
Full Name	probable ATP-dependent RNA helicase DDX60
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029076821.1	XP_028932654.1	probable ATP-dependent RNA helicase DDX60
XM_007673034.1	XP_007671224.1	probable ATP-dependent RNA helicase DDX60

Pathways from BioSystems
Gene Ontology

The following LOC100077465 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100077465 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOc02486	XM_007673034.1 Latest version!	Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077465), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc114297	XM_029076821.1 Latest version!	Ornithorhynchus anatinus DExD/H-box helicase 60 (DDX60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100077465 ( XM_007673034.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007671224.1 Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077465), mRNA.

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CloneID	OOc02486
Clone ID Related Accession (Same CDS sequence)	XM_007673034.1
Accession Version	XM_007673034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1460563200000
Organism	Ornithorhynchus anatinus(platypus)
Product	probable ATP-dependent RNA helicase DDX60
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009105.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGG ACGCTCATGC TGTCCGGGGA GGTGGTGACC AGGAAGTAAA ATTCAGAGTG 
GGGTACGAAG CTAAGAGTTT AGCATGTAAA GAGTCAGAGG AAAATGTCAC CCAAAGTCAC 
CCTGAGGATA TCAAGAAGGC TGTGGAGCCT GTGACCGATC TCCAGAAGGA GCTGCCAGAG 
AAAGGAATGA AGAAAAAAAA AGCGAAAGGG ACCCAGGCAA TAGCTGATCA TGCAGCGTCC 
ACAAAACAAA TTGGAAAAGA GAGGAGCAAG AAGACCAAAA TGAAAACAAA AGTGAAAGGA 
ACTGCAGAAG AAGAAGGTGG AAAAGGAGGA GGAGGAAGAA AGCAAAAAAC AGGAGGAGAA 
GGAGGAGGAG GAGCAGGATC AAAAGCCTGT TCTGAATTTC TGAATGAATT ATCAGAGATC 
ATTTTAAATG AAATACCAAA AGCTGAATAT TCCAGTTTGC TCGCTGACTT TGTAGAATCT 
GAATATTTTC TGTTCGATGG AGATTCTCTA CTCCTCTCCT GCATATGTGA AAAATCACTG 
GTGCTAGGAC AGAACCTTCA CTTCTTCTAT TTAGTCGAAC GCTATTTGAC GGACTTAATA 
AACAAAGGAG CCCAATTTGT CATTATTTTC TTCCAGGATA CTGAAAAAGT ATATTTCAAG 
TTTCCTCAGC TGCTTCTTCT AAGAACAACT TTAATTCTGC ACCTGAAGCA CAATACCGCC 
ATTGACGTCC GGACGGAGTT TTCCAGCTGT TTATCCCCGG AGTGGAAATA TTTCTTGGAA 
GAAAAATACC CGTATTTTTT AATAATTGCA GATAACGGCC TAAATGACCT ACAGACAAAC 
CTCTTAAACA TCTCTATCAT TCAGGCCTGG TCAATGAAAG TCAATGTTGT TCTTATCTCA 
GATCAAGCAT CGGACACAAT CCGAGTTTAT GGACACATTC TTCAGAGTAT GCACAAACAC 
CACATTTTTT TCGCAAAGAA CAGAGCAAAG ATGAAAGAAG TTTATCAGTC ACTGATGAAT 
GAAATGGAAG ATTGCAGTGA TGCTGAATTA GTCCATCTCT TTGGAGGACT GAGAAGGAAG 
ACTATAGAGC AAGAGGCATG GGAAATAGCA GAACGGTTCC AGTGTCTTTG GCCTGAAGGA 
ATCGACGTTC GACGTAACCT CTGTATCACT TCATGTTCGT TGGCTTTGAG AATGTACCAC 
AGCTACCGCT TAGGGAAGAC AGAGATGGAG GAGTCCGAAG AGTTAACCTT GGAGGAAGTA 
GCCGACCTGT GCAGGATGCA CTGCCTCAGT GTGGCTTGTC TCCTTCATCT GCCTCTCGTG 
CAAAGGGCCC AGATGCGTGT CGTCACTTCT AGATGGACTG AGCCAATAGA CTCCTTTTTG 
AACACGAAAC ACTGGTGTGA ATTTTTCGCT TTAAAAACGA TGACATTTTT GAACTTTTCC 
ACTTTAAGAA CGGATGGAAA GTACCTTCCC GACTTAAGTG ATCATCTTCT TTTGAAGAGT 
ATTGCCTATT ACTTTGAAGT CGAAAATAGC CAAGGCCTGC AATTAGAGCT CGGAAAAGCA 
ATTCAGAAGG ATTATGAGTG TCTGTGGGAT TCGATATCAG TGTTAGTTGA CCAGATTGAT 
GTTGAAAGAA CATTTCCTCT TCGAACAACG ACAAAATGTT TTCTTCAAAA GTCAAAAACT 
CCAATAAAAG AACCATCTGA TGAAAAGATG CCCAACCTGG GTTTTATTCC AGTGACATCT 
TCCGTGCTAG ACGCATTCGC TGGAGACATT CTGAAAGGTT TTTCTTTCTT AAGCAGCAAA 
GATCCAGTCG TCACTTCACT CGTGAAGCTA AAGGAATTTG ATGAACTCAA GCACTGGCAT 
TCAGGCAAAC GACTTAGTGA TGATTATGAC AGAACAAGAT GTAACCTGGA AGAAAACAGC 
AACGATCCTC ATTTTCTCAG GAGGAAGGTA AAGTTTCGGG CCTATCAACA GTTTTATGGA 
CAAACCTTGG AAGCTGTCAC TCCAAAAGTC ATTGTAACTC AACCTGCAAA GCCCACGAAA 
GGTTACGATC AAACCAAGAA CAGCAAAAGA TCCAATGAAT CAAAACAAAG CGTCATTGTC 
AGAGAAAATC AGAAAAGGTT GGATGCTCTA AAGGAGCAAA AGGAAAAGGA CCAGTGGATT 
GCCCAATCTG TAGGCATCAT AAGGGCCATT GAAGATAATT TTAGTTTGGG AGTAAGTAAA 
CTGGATGAGT TCCTGAAGAA GTGTAAGAGT GATTCGGTGA AATTTAATGC AGAAAGAGTC 
GGGCTGACTT ACTGTCTGAA AGCGTGGAAA AGACACTGCA AAAAAGAAGG TAAAATATCA 
AAAGATCTAA GTATAGCTGC CCAAGTGATG AGAAGACTTC AGTCCCTAGT GTACAAATAT 
GATGAATTTT TGAAGAAAAA AGATTGTGAA CTTATAGTCA AATGCCTTAA ATATTTAGGA 
TTTGATAATT TGTTGGATTT TAAGGATTTT TCTCAGGAGG TGGAAGATGA CAGGACTGTA 
AAGAAAAAAG CCAAGTACTC TGTTGATATG GGTTCAGCAC GATTCCAGCT CCTGCACATG 
GGCCATTTCT TGATGCGAAA TGAGAGAACA GATCCAGACC CCAGGGTCCA GGATTTCATT 
CCAGACACAT GGCAGCGAGA GCTTCTCGAC GTGGTGGATA ACAATGAGTC TGCAGTGATC 
GTTGCCCCAA CGTCTTCGGG GAAAACATAC GCCTCCTACT ACTGCATGGA GAGAGTGCTG 
AAAGAGAGCG ATGAAGGGGT GGCCGTATAT GTGGCGCCTA CGAAGGCCCT TGTGAACCAA 
GTGTCAGCAA CTGTTCAGAA TCGATTCACT AAAAAACTGC CCGTAGGTCA AGCTCTGTGT 
GGAACTTTCA CCAGGGATTA TCGCCAGGAT GTGCTGAACT GCCAGGTACT CGTGACAGTG 
CCAGCCTGTT TGGAAATCCT TCTGCTCTCT CCCCATCGTC AAGCATGGGT GAAAAGAATC 
AGATACGTTA TATTTGATGA GGTCCATTGT CTTGGTGGGG AAATTGGAGC AGAAGTTTGG 
GAGCACCTCC TTGTTATGAT CCGATGCCCT TTTTTAGCTC TCTCTGCTAC TATCAGTAAC 
CCGGGACACC TCACTAGGTG GCTCCAATCT GTCAAAACAT ATTGGCAAAA CGCAGATCGG 
ATAATAGATG GAAGTCTCCA TTCTGACCTG GCTTCTAGAT GTGGCTCCAA AGTTCAAAAA 
AAATTATCTG AAGCAAAGAG ATCATACAAA GTTAGATTGG TTCTGTACGG CAAGAGATAC 
AATCATTTGG AAAAATACGT CTGCTCCTTT AAAGGTGATG ACATCGTCAC GGATCACTAT 
CATCCATGTG CTGCCCTCAC CACAGATCAT ATTAAAGTCC ATGGATTTCC TTCAGATCTT 
ACTCTTTCTC CACAAGAAAG CCTCCAGCTT TATGACATGA TGGTTAGCGT TTGGGAGAAA 
TGGCCTCATG CCCAGCAATT AGATCCGGAA GAATTTCAAC ATTTTAAAGA TAAAGTAGCA 
ATCTCAAGGG CAGATGCCCA GAAATATGAA GCAAGTCTAA AGAAGGAACT CAAAAAATGG 
ATTGAACATG GTCATACAGA GAAGGTCAGA GAGGTACTGA ATAATTTGAA ACCTAACTGT 
GTTCCTTGTT CGCAAGAAAT GGCAGTTAAC TTCCCACTTC TTGTTGAAAA ACTAAGGAAA 
ATGGACAAAT TGCCTGCTCT GTTCTTCATT TTTAAAATAA CAGATGTGGA ACTGAGGGCT 
AATGAAGTAT ACAAGTCTCT GGAGGTGAAG CAGGAAGAAC AGCAGTCTCC CAAGGCTGAG 
AAAGAGGTCT CTAACCTAGA CCAAAGACTG AAAAAAGTGA CAAAATCCAT TGAGAAACAG 
CCCAAAATAC CTCCAAAACA CATCTCGAAA GCGGATCAAC TCCTGGTACA AGAAGCCGAG 
TATCAGCAGT TGCTCCAGAA GCTGGAGAAG CTGAAAAAGA TCCCCGAAGA CTGTACTTAC 
GCCAACCTAA AGGCTATCAA AAATCAGACT TTGTATAAAG TGTTTCACCA AGTGAAATTC 
ACATATAAAA GTGAAGAACT GCAAACTTTG GCACTGAGGG GCATTGGTTA CCATCACGGT 
GCTCTGAATT TCAGAGGAAG ACAGCTGGTT GAGATACTCT TCAGGGAGGG GTTGATCAAG 
GTAGTGACAG CTACGGGGAC GCTTGCATTA GGAATCAATA TGCCGTGCAA ATCTGTCGTT 
TTCGCTCAAA ATTCAATCTT CTTGGATGCT TTGAACTACA GACAGATGTC AGGGCGTGCA 
GGAAGGCGAG GCCAAGACCT GAAAGGAAGT GTGTTTTTCT ATAATATTCC ACTGCCCAAA 
ATAGAAAAAC TCATTAAATC CAACGTCCCT AAGCTAAGAG GCCACTTCCC GCTGGGCATA 
TCCCTGGTTC TTCGCCTCAT GCTGTTGGCT TCCAAAGGAG ATGATCGAAA GGATGCAGAA 
GCAAAGGTTT TATCAGTGCT GAAGCATTCC CTGCTGACTT TCAGCCGACC CAGAGCCATG 
GAGATGTTGA AAATATTCTT CTTGTTTTCT TTGCAGTTTC TCGTTAAAGA GGGCTATTTA 
GACCGAGAAT GTAATCCCAT GGGTTTTGCT GGACTTGTAT CACATTTGCA TTACCATGAA 
CCGGCTAATT TTCTCCTGGT CAGTTTTCTG GTGAAAGGCT TATTACACAA ACTCTGCAAA 
CCAATCAAGA AAGGCTCTTC AGTGTTTTCT GAAGATGTGA TGGAAAAGCT AATGCTGATA 
CTGGCAAACT TGTTTGGAAG GAAATACATT CTAGCCAAGT ATTTTGAAGC TAAAAGAAAA 
TTTCAGCAGT CAAAGGTGTT TCTTGAAGAT CTCCCCAAGG ATTTTGAAGC AGCCGTATTT 
GAGTACAACG TAAAAATAAA AAACGACTTT TGCACTTTTA TACGGATTGT TTCCAAACTG 
GCTGACATGA AGCAGGAGTA CCAACTCCCC TTGTCAAAAA TAGATTTCAC GGATGAAATT 
TACGCGGATT CTGAACTTTT GTCTCACTTG ATGAGCTGCA AAGAGGGGAG GACAGCCATT 
TCACCCTTCG TGTGCCTATC CGGGTACACG GATCGTGATT TACTTCACTC GGATTCTCTC 
AGCAGTGTGG TACTGCAATC AGTTGGTGTT AATGACTCCC TTGTTCCTCT CCTGGACTTA 
AAGAAATCTG ACAGTAGAGG AAGAAGAATG CCACTTAACG CATATGCACT TGACTTTTAC 
AAGCACGGAT CGATAACGGC ATTGTCAGAA GACAATGGGT TGCATAGAGG AGAAGTTTTC 
CTTCTCCTGA GAGATTTTTT GCTCACCATA CAATCTATCA GCATCTCTCT GAGTGAACTG 
TGTGAAAAAG AAGACGATGA CGTGGTCCTC GCCTTTAAAC AGCTGAGTAG TAGCTTCAAA 
GCAAAACTGG ATAAAGTCAG TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007671224.1
CDS	40..5526
Translation	MQEDAHAVRGGGDQEVKFRVGYEAKSLACKESEENVTQSHPEDIKKAVEPVTDLQKELPEKGMKKKKAKGTQAIADHAASTKQIGKERSKKTKMKTKVKGTAEEEGGKGGGGRKQKTGGEGGGGAGSKACSEFLNELSEIILNEIPKAEYSSLLADFVESEYFLFDGDSLLLSCICEKSLVLGQNLHFFYLVERYLTDLINKGAQFVIIFFQDTEKVYFKFPQLLLLRTTLILHLKHNTAIDVRTEFSSCLSPEWKYFLEEKYPYFLIIADNGLNDLQTNLLNISIIQAWSMKVNVVLISDQASDTIRVYGHILQSMHKHHIFFAKNRAKMKEVYQSLMNEMEDCSDAELVHLFGGLRRKTIEQEAWEIAERFQCLWPEGIDVRRNLCITSCSLALRMYHSYRLGKTEMEESEELTLEEVADLCRMHCLSVACLLHLPLVQRAQMRVVTSRWTEPIDSFLNTKHWCEFFALKTMTFLNFSTLRTDGKYLPDLSDHLLLKSIAYYFEVENSQGLQLELGKAIQKDYECLWDSISVLVDQIDVERTFPLRTTTKCFLQKSKTPIKEPSDEKMPNLGFIPVTSSVLDAFAGDILKGFSFLSSKDPVVTSLVKLKEFDELKHWHSGKRLSDDYDRTRCNLEENSNDPHFLRRKVKFRAYQQFYGQTLEAVTPKVIVTQPAKPTKGYDQTKNSKRSNESKQSVIVRENQKRLDALKEQKEKDQWIAQSVGIIRAIEDNFSLGVSKLDEFLKKCKSDSVKFNAERVGLTYCLKAWKRHCKKEGKISKDLSIAAQVMRRLQSLVYKYDEFLKKKDCELIVKCLKYLGFDNLLDFKDFSQEVEDDRTVKKKAKYSVDMGSARFQLLHMGHFLMRNERTDPDPRVQDFIPDTWQRELLDVVDNNESAVIVAPTSSGKTYASYYCMERVLKESDEGVAVYVAPTKALVNQVSATVQNRFTKKLPVGQALCGTFTRDYRQDVLNCQVLVTVPACLEILLLSPHRQAWVKRIRYVIFDEVHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPGHLTRWLQSVKTYWQNADRIIDGSLHSDLASRCGSKVQKKLSEAKRSYKVRLVLYGKRYNHLEKYVCSFKGDDIVTDHYHPCAALTTDHIKVHGFPSDLTLSPQESLQLYDMMVSVWEKWPHAQQLDPEEFQHFKDKVAISRADAQKYEASLKKELKKWIEHGHTEKVREVLNNLKPNCVPCSQEMAVNFPLLVEKLRKMDKLPALFFIFKITDVELRANEVYKSLEVKQEEQQSPKAEKEVSNLDQRLKKVTKSIEKQPKIPPKHISKADQLLVQEAEYQQLLQKLEKLKKIPEDCTYANLKAIKNQTLYKVFHQVKFTYKSEELQTLALRGIGYHHGALNFRGRQLVEILFREGLIKVVTATGTLALGINMPCKSVVFAQNSIFLDALNYRQMSGRAGRRGQDLKGSVFFYNIPLPKIEKLIKSNVPKLRGHFPLGISLVLRLMLLASKGDDRKDAEAKVLSVLKHSLLTFSRPRAMEMLKIFFLFSLQFLVKEGYLDRECNPMGFAGLVSHLHYHEPANFLLVSFLVKGLLHKLCKPIKKGSSVFSEDVMEKLMLILANLFGRKYILAKYFEAKRKFQQSKVFLEDLPKDFEAAVFEYNVKIKNDFCTFIRIVSKLADMKQEYQLPLSKIDFTDEIYADSELLSHLMSCKEGRTAISPFVCLSGYTDRDLLHSDSLSSVVLQSVGVNDSLVPLLDLKKSDSRGRRMPLNAYALDFYKHGSITALSEDNGLHRGEVFLLLRDFLLTIQSISISLSELCEKEDDDVVLAFKQLSSSFKAKLDKVSD

Target ORF information:

RefSeq Version	XM_007673034.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077465), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007673034.1

ORF Insert Sequence:

ATGCAAGAGG ACGCTCATGC TGTCCGGGGA GGTGGTGACC AGGAAGTAAA ATTCAGAGTG 
GGGTACGAAG CTAAGAGTTT AGCATGTAAA GAGTCAGAGG AAAATGTCAC CCAAAGTCAC 
CCTGAGGATA TCAAGAAGGC TGTGGAGCCT GTGACCGATC TCCAGAAGGA GCTGCCAGAG 
AAAGGAATGA AGAAAAAAAA AGCGAAAGGG ACCCAGGCAA TAGCTGATCA TGCAGCGTCC 
ACAAAACAAA TTGGAAAAGA GAGGAGCAAG AAGACCAAAA TGAAAACAAA AGTGAAAGGA 
ACTGCAGAAG AAGAAGGTGG AAAAGGAGGA GGAGGAAGAA AGCAAAAAAC AGGAGGAGAA 
GGAGGAGGAG GAGCAGGATC AAAAGCCTGT TCTGAATTTC TGAATGAATT ATCAGAGATC 
ATTTTAAATG AAATACCAAA AGCTGAATAT TCCAGTTTGC TCGCTGACTT TGTAGAATCT 
GAATATTTTC TGTTCGATGG AGATTCTCTA CTCCTCTCCT GCATATGTGA AAAATCACTG 
GTGCTAGGAC AGAACCTTCA CTTCTTCTAT TTAGTCGAAC GCTATTTGAC GGACTTAATA 
AACAAAGGAG CCCAATTTGT CATTATTTTC TTCCAGGATA CTGAAAAAGT ATATTTCAAG 
TTTCCTCAGC TGCTTCTTCT AAGAACAACT TTAATTCTGC ACCTGAAGCA CAATACCGCC 
ATTGACGTCC GGACGGAGTT TTCCAGCTGT TTATCCCCGG AGTGGAAATA TTTCTTGGAA 
GAAAAATACC CGTATTTTTT AATAATTGCA GATAACGGCC TAAATGACCT ACAGACAAAC 
CTCTTAAACA TCTCTATCAT TCAGGCCTGG TCAATGAAAG TCAATGTTGT TCTTATCTCA 
GATCAAGCAT CGGACACAAT CCGAGTTTAT GGACACATTC TTCAGAGTAT GCACAAACAC 
CACATTTTTT TCGCAAAGAA CAGAGCAAAG ATGAAAGAAG TTTATCAGTC ACTGATGAAT 
GAAATGGAAG ATTGCAGTGA TGCTGAATTA GTCCATCTCT TTGGAGGACT GAGAAGGAAG 
ACTATAGAGC AAGAGGCATG GGAAATAGCA GAACGGTTCC AGTGTCTTTG GCCTGAAGGA 
ATCGACGTTC GACGTAACCT CTGTATCACT TCATGTTCGT TGGCTTTGAG AATGTACCAC 
AGCTACCGCT TAGGGAAGAC AGAGATGGAG GAGTCCGAAG AGTTAACCTT GGAGGAAGTA 
GCCGACCTGT GCAGGATGCA CTGCCTCAGT GTGGCTTGTC TCCTTCATCT GCCTCTCGTG 
CAAAGGGCCC AGATGCGTGT CGTCACTTCT AGATGGACTG AGCCAATAGA CTCCTTTTTG 
AACACGAAAC ACTGGTGTGA ATTTTTCGCT TTAAAAACGA TGACATTTTT GAACTTTTCC 
ACTTTAAGAA CGGATGGAAA GTACCTTCCC GACTTAAGTG ATCATCTTCT TTTGAAGAGT 
ATTGCCTATT ACTTTGAAGT CGAAAATAGC CAAGGCCTGC AATTAGAGCT CGGAAAAGCA 
ATTCAGAAGG ATTATGAGTG TCTGTGGGAT TCGATATCAG TGTTAGTTGA CCAGATTGAT 
GTTGAAAGAA CATTTCCTCT TCGAACAACG ACAAAATGTT TTCTTCAAAA GTCAAAAACT 
CCAATAAAAG AACCATCTGA TGAAAAGATG CCCAACCTGG GTTTTATTCC AGTGACATCT 
TCCGTGCTAG ACGCATTCGC TGGAGACATT CTGAAAGGTT TTTCTTTCTT AAGCAGCAAA 
GATCCAGTCG TCACTTCACT CGTGAAGCTA AAGGAATTTG ATGAACTCAA GCACTGGCAT 
TCAGGCAAAC GACTTAGTGA TGATTATGAC AGAACAAGAT GTAACCTGGA AGAAAACAGC 
AACGATCCTC ATTTTCTCAG GAGGAAGGTA AAGTTTCGGG CCTATCAACA GTTTTATGGA 
CAAACCTTGG AAGCTGTCAC TCCAAAAGTC ATTGTAACTC AACCTGCAAA GCCCACGAAA 
GGTTACGATC AAACCAAGAA CAGCAAAAGA TCCAATGAAT CAAAACAAAG CGTCATTGTC 
AGAGAAAATC AGAAAAGGTT GGATGCTCTA AAGGAGCAAA AGGAAAAGGA CCAGTGGATT 
GCCCAATCTG TAGGCATCAT AAGGGCCATT GAAGATAATT TTAGTTTGGG AGTAAGTAAA 
CTGGATGAGT TCCTGAAGAA GTGTAAGAGT GATTCGGTGA AATTTAATGC AGAAAGAGTC 
GGGCTGACTT ACTGTCTGAA AGCGTGGAAA AGACACTGCA AAAAAGAAGG TAAAATATCA 
AAAGATCTAA GTATAGCTGC CCAAGTGATG AGAAGACTTC AGTCCCTAGT GTACAAATAT 
GATGAATTTT TGAAGAAAAA AGATTGTGAA CTTATAGTCA AATGCCTTAA ATATTTAGGA 
TTTGATAATT TGTTGGATTT TAAGGATTTT TCTCAGGAGG TGGAAGATGA CAGGACTGTA 
AAGAAAAAAG CCAAGTACTC TGTTGATATG GGTTCAGCAC GATTCCAGCT CCTGCACATG 
GGCCATTTCT TGATGCGAAA TGAGAGAACA GATCCAGACC CCAGGGTCCA GGATTTCATT 
CCAGACACAT GGCAGCGAGA GCTTCTCGAC GTGGTGGATA ACAATGAGTC TGCAGTGATC 
GTTGCCCCAA CGTCTTCGGG GAAAACATAC GCCTCCTACT ACTGCATGGA GAGAGTGCTG 
AAAGAGAGCG ATGAAGGGGT GGCCGTATAT GTGGCGCCTA CGAAGGCCCT TGTGAACCAA 
GTGTCAGCAA CTGTTCAGAA TCGATTCACT AAAAAACTGC CCGTAGGTCA AGCTCTGTGT 
GGAACTTTCA CCAGGGATTA TCGCCAGGAT GTGCTGAACT GCCAGGTACT CGTGACAGTG 
CCAGCCTGTT TGGAAATCCT TCTGCTCTCT CCCCATCGTC AAGCATGGGT GAAAAGAATC 
AGATACGTTA TATTTGATGA GGTCCATTGT CTTGGTGGGG AAATTGGAGC AGAAGTTTGG 
GAGCACCTCC TTGTTATGAT CCGATGCCCT TTTTTAGCTC TCTCTGCTAC TATCAGTAAC 
CCGGGACACC TCACTAGGTG GCTCCAATCT GTCAAAACAT ATTGGCAAAA CGCAGATCGG 
ATAATAGATG GAAGTCTCCA TTCTGACCTG GCTTCTAGAT GTGGCTCCAA AGTTCAAAAA 
AAATTATCTG AAGCAAAGAG ATCATACAAA GTTAGATTGG TTCTGTACGG CAAGAGATAC 
AATCATTTGG AAAAATACGT CTGCTCCTTT AAAGGTGATG ACATCGTCAC GGATCACTAT 
CATCCATGTG CTGCCCTCAC CACAGATCAT ATTAAAGTCC ATGGATTTCC TTCAGATCTT 
ACTCTTTCTC CACAAGAAAG CCTCCAGCTT TATGACATGA TGGTTAGCGT TTGGGAGAAA 
TGGCCTCATG CCCAGCAATT AGATCCGGAA GAATTTCAAC ATTTTAAAGA TAAAGTAGCA 
ATCTCAAGGG CAGATGCCCA GAAATATGAA GCAAGTCTAA AGAAGGAACT CAAAAAATGG 
ATTGAACATG GTCATACAGA GAAGGTCAGA GAGGTACTGA ATAATTTGAA ACCTAACTGT 
GTTCCTTGTT CGCAAGAAAT GGCAGTTAAC TTCCCACTTC TTGTTGAAAA ACTAAGGAAA 
ATGGACAAAT TGCCTGCTCT GTTCTTCATT TTTAAAATAA CAGATGTGGA ACTGAGGGCT 
AATGAAGTAT ACAAGTCTCT GGAGGTGAAG CAGGAAGAAC AGCAGTCTCC CAAGGCTGAG 
AAAGAGGTCT CTAACCTAGA CCAAAGACTG AAAAAAGTGA CAAAATCCAT TGAGAAACAG 
CCCAAAATAC CTCCAAAACA CATCTCGAAA GCGGATCAAC TCCTGGTACA AGAAGCCGAG 
TATCAGCAGT TGCTCCAGAA GCTGGAGAAG CTGAAAAAGA TCCCCGAAGA CTGTACTTAC 
GCCAACCTAA AGGCTATCAA AAATCAGACT TTGTATAAAG TGTTTCACCA AGTGAAATTC 
ACATATAAAA GTGAAGAACT GCAAACTTTG GCACTGAGGG GCATTGGTTA CCATCACGGT 
GCTCTGAATT TCAGAGGAAG ACAGCTGGTT GAGATACTCT TCAGGGAGGG GTTGATCAAG 
GTAGTGACAG CTACGGGGAC GCTTGCATTA GGAATCAATA TGCCGTGCAA ATCTGTCGTT 
TTCGCTCAAA ATTCAATCTT CTTGGATGCT TTGAACTACA GACAGATGTC AGGGCGTGCA 
GGAAGGCGAG GCCAAGACCT GAAAGGAAGT GTGTTTTTCT ATAATATTCC ACTGCCCAAA 
ATAGAAAAAC TCATTAAATC CAACGTCCCT AAGCTAAGAG GCCACTTCCC GCTGGGCATA 
TCCCTGGTTC TTCGCCTCAT GCTGTTGGCT TCCAAAGGAG ATGATCGAAA GGATGCAGAA 
GCAAAGGTTT TATCAGTGCT GAAGCATTCC CTGCTGACTT TCAGCCGACC CAGAGCCATG 
GAGATGTTGA AAATATTCTT CTTGTTTTCT TTGCAGTTTC TCGTTAAAGA GGGCTATTTA 
GACCGAGAAT GTAATCCCAT GGGTTTTGCT GGACTTGTAT CACATTTGCA TTACCATGAA 
CCGGCTAATT TTCTCCTGGT CAGTTTTCTG GTGAAAGGCT TATTACACAA ACTCTGCAAA 
CCAATCAAGA AAGGCTCTTC AGTGTTTTCT GAAGATGTGA TGGAAAAGCT AATGCTGATA 
CTGGCAAACT TGTTTGGAAG GAAATACATT CTAGCCAAGT ATTTTGAAGC TAAAAGAAAA 
TTTCAGCAGT CAAAGGTGTT TCTTGAAGAT CTCCCCAAGG ATTTTGAAGC AGCCGTATTT 
GAGTACAACG TAAAAATAAA AAACGACTTT TGCACTTTTA TACGGATTGT TTCCAAACTG 
GCTGACATGA AGCAGGAGTA CCAACTCCCC TTGTCAAAAA TAGATTTCAC GGATGAAATT 
TACGCGGATT CTGAACTTTT GTCTCACTTG ATGAGCTGCA AAGAGGGGAG GACAGCCATT 
TCACCCTTCG TGTGCCTATC CGGGTACACG GATCGTGATT TACTTCACTC GGATTCTCTC 
AGCAGTGTGG TACTGCAATC AGTTGGTGTT AATGACTCCC TTGTTCCTCT CCTGGACTTA 
AAGAAATCTG ACAGTAGAGG AAGAAGAATG CCACTTAACG CATATGCACT TGACTTTTAC 
AAGCACGGAT CGATAACGGC ATTGTCAGAA GACAATGGGT TGCATAGAGG AGAAGTTTTC 
CTTCTCCTGA GAGATTTTTT GCTCACCATA CAATCTATCA GCATCTCTCT GAGTGAACTG 
TGTGAAAAAG AAGACGATGA CGTGGTCCTC GCCTTTAAAC AGCTGAGTAG TAGCTTCAAA 
GCAAAACTGG ATAAAGTCAG TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100077465 ( XM_029076821.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028932654.1 Ornithorhynchus anatinus DExD/H-box helicase 60 (DDX60), mRNA.

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CloneID	OOc114297
Clone ID Related Accession (Same CDS sequence)	XM_029076821.1
Accession Version	XM_029076821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	probable ATP-dependent RNA helicase DDX60
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041739.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGG ACGCTCATGC TGTCCGGGGA GGTGGTGACC AGGAAGTAAA ATTCAGAGTG 
GGGTACGAAG CTAAGAGTTT AGCATGTAAA GAGTCAGAGG AAAATGTCAC CCAAAGTCAC 
CCTGAGGATA TCAAGAAGGC TGTGGAGCCT GTGACCGATC TCCAGAAGGA GCTGCCAGAG 
AAAGGAATGA AGAAAAAAAA AGCGAAAGGG ACCCAGGCAA TAGCTGATCA TGCAGCGTCC 
ACAAAACAAA TTGGAAAAGA GAGGAGCAAG AAGACCAAAA TGAAAACAAA AGTGAAAGGA 
ACTGCAGAAG AAGAAGGTGG AAAAGGAGGA GGAGGAAGAA AGCAAAAAAC AGGAGGAGAA 
GGAGGAGGAG GAGCAGGATC AAAAGCCTGT TCTGAATTTC TGAATGAATT ATCAGAGATC 
ATTTTAAATG AAATACCAAA AGCTGAATAT TCCAGTTTGC TCGCTGACTT TGTAGAATCT 
GAATATTTTC TGTTCGATGG AGATTCTCTA CTCCTCTCCT GCATATGTGA AAAATCACTG 
GTGCTAGGAC AGAACCTTCA CTTCTTCTAT TTAGTCGAAC GCTATTTGAC GGACTTAATA 
AACAAAGGAG CCCAATTTGT CATTATTTTC TTCCAGGATA CTGAAAAAGT ATATTTCAAG 
TTTCCTCAGC TGCTTCTTCT AAGAACAACT TTAATTCTGC ACCTGAAGCA CAATACCGCC 
ATTGACGTCC GGACGGAGTT TTCCAGCTGT TTATCCCCGG AGTGGAAATA TTTCTTGGAA 
GAAAAATACC CGTATTTTTT AATAATTGCA GATAACGGCC TAAATGACCT ACAGACAAAC 
CTCTTAAACA TCTCTATCAT TCAGGCCTGG TCAATGAAAG TCAATGTTGT TCTTATCTCA 
GATCAAGCAT CGGACACAAT CCGAGTTTAT GGACACATTC TTCAGAGTAT GCACAAACAC 
CACATTTTTT TCGCAAAGAA CAGAGCAAAG ATGAAAGAAG TTTATCAGTC ACTGATGAAT 
GAAATGGAAG ATTGCAGTGA TGCTGAATTA GTCCATCTCT TTGGAGGACT GAGAAGGAAG 
ACTATAGAGC AAGAGGCATG GGAAATAGCA GAACGGTTCC AGTGTCTTTG GCCTGAAGGA 
ATCGACGTTC GACGTAACCT CTGTATCACT TCATGTTCGT TGGCTTTGAG AATGTACCAC 
AGCTACCGCT TAGGGAAGAC AGAGATGGAG GAGTCCGAAG AGTTAACCTT GGAGGAAGTA 
GCCGACCTGT GCAGGATGCA CTGCCTCAGT GTGGCTTGTC TCCTTCATCT GCCTCTCGTG 
CAAAGGGCCC AGATGCGTGT CGTCACTTCT AGATGGACTG AGCCAATAGA CTCCTTTTTG 
AACACGAAAC ACTGGTGTGA ATTTTTCGCT TTAAAAACGA TGACATTTTT GAACTTTTCC 
ACTTTAAGAA CGGATGGAAA GTACCTTCCC GACTTAAGTG ATCATCTTCT TTTGAAGAGT 
ATTGCCTATT ACTTTGAAGT CGAAAATAGC CAAGGCCTGC AATTAGAGCT CGGAAAAGCA 
ATTCAGAAGG ATTATGAGTG TCTGTGGGAT TCGATATCAG TGTTAGTTGA CCAGATTGAT 
GTTGAAAGAA CATTTCCTCT TCGAACAACG ACAAAATGTT TTCTTCAAAA GTCAAAAACT 
CCAATAAAAG AACCATCTGA TGAAAAGATG CCCAACCTGG GTTTTATTCC AGTGACATCT 
TCCGTGCTAG ACGCATTCGC TGGAGACATT CTGAAAGGTC TTTCTTTCTT AAGCAGCAAA 
GATCCAGTCG TCACTTCACT CGTGAAGCTA AAGGAATTTG ATGAACTCAA GCACTGGCAT 
TCAGGCAAAC GACTTAGTGA TGATTATGAC AGAACAAGAT GTAACCTGGA AGAAAACAGC 
AACGATCCTC ATTTTCTCAG GAGGAAGGTA AAGTTTCGGG CCTATCAACA GTTTTATGGA 
CAAACCTTGG AAGCTGTCAC TCCAAAAGTC ATTGTAACTC AACCTGCAAA GCCCACGAAA 
GGTTACGATC AAACCAAGAA CAGCAAAAGA TCCAATGAAT CAAAACAAAG CGTCATTGTC 
AGAGAAAATC AGAAAAGGTT GGATGCTCTA AAGGAGCAAA AGGAAAAGGA CCAGTGGATT 
GCCCAATCTG TAGGCATCAT AAGGGCCATT GAAGATAATT TTAGTTTGGG AGTAAGTAAA 
CTGGATGAGT TCCTGAAGAA GTGTAAGAGT GATTCGGTGA AATTTAATGC AGAAAGAGTC 
GGGCTGACTT ACTGTCTGAA AGCGTGGAAA AGACACTGCA AAAAAGAAGG TAAAATATCA 
AAAGATCTAA GTATAGCTGC CCAAGTGATG AGAAGACTTC AGTCCCTAGT GTACAAATAT 
GATGAATTTT TGAAGAAAAA AGATTGTGAA CTTATAGTCA AATGCCTTAA ATATTTAGGA 
TTTGATAATT TGTTGGATTT TAAGGATTTT TCTCAGGAGG TGGAAGATGA CAGGACTGTA 
AAGAAAAAAG CCAAGTACTC TGTTGATATG GGTTCAGCAC GATTCCAGCT CCTGCACATG 
GGCCATTTCT TGATGCGAAA TGAGAGAACA GATCCAGACC CCAGGGTCCA GGATTTCATT 
CCAGACACAT GGCAGCGAGA GCTTCTCGAC GTGGTGGATA ACAATGAGTC TGCAGTGATC 
GTTGCCCCAA CGTCTTCGGG GAAAACATAC GCCTCCTACT ACTGCATGGA GAGAGTGCTG 
AAAGAGAGCG ATGAAGGGGT GGCCGTATAT GTGGCGCCTA CGAAGGCCCT TGTGAACCAA 
GTGTCAGCAA CTGTTCAGAA TCGATTCACT AAAAAACTGC CCGTAGGTCA AGCTCTGTGT 
GGAACTTTCA CCAGGGATTA TCGCCAGGAT GTGCTGAACT GCCAGGTACT CGTGACAGTG 
CCAGCCTGTT TGGAAATCCT TCTGCTCTCT CCCCATCGTC AAGCATGGGT GAAAAGAATC 
AGATACGTTA TATTTGATGA GGTCCATTGT CTTGGTGGGG AAATTGGAGC AGAAGTTTGG 
GAGCACCTCC TTGTTATGAT CCGATGCCCT TTTTTAGCTC TCTCTGCTAC TATCAGTAAC 
CCGGGACACC TCACTAGGTG GCTCCAATCT GTCAAAACAT ATTGGCAAAA CGCAGATCGG 
ATAATAGATG GAAGTCTCCA TTCTGACCTG GCTTCTAGAT GTGGCTCCAA AGTTCAAAAA 
AAATTATCTG AAGCAAAGAG ATCATACAAA GTTAGATTGG TTCTGTACGG CGAGAGATAC 
AATCATTTGG AAAAATACGT CTGCTCCTTT AAAGGTGATG ACATCGTCAC GGATCACTAT 
CATCCATGTG CTGCCCTCAC CACAGATCAT ATTAAAGTCC ATGGATTTCC TTCAGATCTT 
ACTCTTTCTC CACAAGAAAG CCTCCAGCTT TATGACATGA TGGTTAGCGT TTGGGAGAAA 
TGGCCTCATG CCCAGCAATT AGATCCGGAA GAATTTCAAC ATTTTAAAGA TAAAGTAGCA 
ATCTCAAGGG CAGATGCCCA GAAATATGAA GCAAGTCTAA AGAAGGAACT CAAAAAATGG 
ATTGAACATG GTCATACAGA GAAGGTCAGA GAGGTACTGA ATAATTTGAA ACCTAACTGT 
GTTCCTTGTT CGCAAGAAAT GGCAGTTAAC TTCCCACTTC TTGTTGAAAA ACTAAGGAAA 
ATGGACAAAT TGCCTGCTCT GTTCTTCATT TTTAAAATAA CAGATGTGGA ACTGAGGGCT 
AATGAAGTAT ACAAGTCTCT GGAGGTGAAG CAGGAAGAAC AGCAGTCTCC CAAGGCTGAG 
AAAGAGGTCT CTAACCTAGA CCAAAGACTG AAAAAAGTGA CAAAATCCAT TGAGAAACAG 
CCCAAAATAC CTCCAAAACA CATCTCGAAA GCGGATCAAC TCCTGGTACA AGAAGCCGAG 
TATCAGCAGT TGCTCCAGAA GCTGGAGAAG CTGAAAAAGA TCCCCGAAGA CTGTACTTAC 
GCCAACCTAA AGGCTATCAA AAATCAGACT TTGTATAAAG TGTTTCACCA AGTGAAATTC 
ACATATAAAA GTGAAGAACT GCAAACTTTG GCACTGAGGG GCATTGGTTA CCATCACGGT 
GCTCTGAATT TCAGAGGAAG ACAGCTGGTT GAGATACTCT TCAGGGAGGG GTTGATCAAG 
GTAGTGACAG CTACGGGGAC GCTTGCATTA GGAATCAATA TGCCGTGCAA ATCTGTCGTT 
TTCGCTCAAA ATTCAATCTT CTTGGATGCT TTGAACTACA GACAGATGTC AGGGCGTGCA 
GGAAGGCGAG GCCAAGACCT GAAAGGAAGT GTGTTTTTCT ATAATATTCC ACTGCCCAAA 
ATAGAAAAAC TCATTAAATC CAACGTCCCT AAGCTAAGAG GCCACTTCCC GCTGGGCATA 
TCCCTGGTTC TTCGCCTCAT GCTGTTGGCT TCCAAAGGAG ATGATCGAAA GGATGCAGAA 
GCAAAGGTTT TATCAGTGCT GAAGCATTCC CTGCTGACTT TCAGCCGACC CAGAGCCATG 
GAGATGTTGA AAATATTCTT CTTGTTTTCT TTGCAGTTTC TCGTTAAAGA GGGCTATTTA 
GACCGAGAAT GTAATCCCAT GGGTTTTGCT GGACTTGTAT CACATTTGCA TTACCATGAA 
CCGGCTAATT TTCTCCTGGT CAGTTTTCTG GTGAAAGGCT TATTACACAA ACTCTGCAAA 
CCAATCAAGA AAGGCTCTTC AGTGTTTTCT GAAGATGTGA TGGAAAAGCT AATGCTGATA 
CTGGCAAACT TGTTTGGAAG GAAATACATT CTAGCCAAGT ATTTTGAAGC TAAAAGAAAA 
TTTCAGCAGT CAAAGGTGTT TCTTGAAGAT CTCCCCAAGG ATTTTGAAGC AGCCGTATTT 
GAGTACAACG TAAAAATAAA AAACGACTTT TGCACTTTTA TACGGATTGT TTCCAAACTG 
GCTGACATGA AGCAGGAGTA CCAACTCCCC TTGTCAAAAA TAGATTTCAC GGATGAAATT 
TACGCGGATT CTGAACTTTT GTCTCACTTG ATGAGCTGCA AAGAGGGGAG GACAGCCATT 
TCACCCTTCG TGTGCCTATC CGGGTACACG GATCGTGATT TACTTCACTC GGATTCTCTC 
AGCAGTGTGG TACTGCAATC AGTTGGTGTT AATGACTCCC TTGTTCCTCT CCTGGACTTA 
AAGAAATCTG ACAGTAGAGG AAGAAGAATG CCACTTAACG CATATGCACT TGACTTTTAC 
AAGCACGGAT CGATAACGGC ATTGTCAGAA GACAATGGGT TGCATAGAGG AGAAGTTTTC 
CTTCTCCTGA GAGATTTTTT GCTCACCATA CAATCTATCA GCATCTCTCT GAGTGAACTG 
TGTGAAAAAG AAGACGATGA CGTGGTCCTC GCCTTTAAAC AGCTGAGTAG TAGCTTCAAA 
GCAAAACTGG ATAAAGTCAG TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028932654.1
CDS	123..5609
Translation	MQEDAHAVRGGGDQEVKFRVGYEAKSLACKESEENVTQSHPEDIKKAVEPVTDLQKELPEKGMKKKKAKGTQAIADHAASTKQIGKERSKKTKMKTKVKGTAEEEGGKGGGGRKQKTGGEGGGGAGSKACSEFLNELSEIILNEIPKAEYSSLLADFVESEYFLFDGDSLLLSCICEKSLVLGQNLHFFYLVERYLTDLINKGAQFVIIFFQDTEKVYFKFPQLLLLRTTLILHLKHNTAIDVRTEFSSCLSPEWKYFLEEKYPYFLIIADNGLNDLQTNLLNISIIQAWSMKVNVVLISDQASDTIRVYGHILQSMHKHHIFFAKNRAKMKEVYQSLMNEMEDCSDAELVHLFGGLRRKTIEQEAWEIAERFQCLWPEGIDVRRNLCITSCSLALRMYHSYRLGKTEMEESEELTLEEVADLCRMHCLSVACLLHLPLVQRAQMRVVTSRWTEPIDSFLNTKHWCEFFALKTMTFLNFSTLRTDGKYLPDLSDHLLLKSIAYYFEVENSQGLQLELGKAIQKDYECLWDSISVLVDQIDVERTFPLRTTTKCFLQKSKTPIKEPSDEKMPNLGFIPVTSSVLDAFAGDILKGLSFLSSKDPVVTSLVKLKEFDELKHWHSGKRLSDDYDRTRCNLEENSNDPHFLRRKVKFRAYQQFYGQTLEAVTPKVIVTQPAKPTKGYDQTKNSKRSNESKQSVIVRENQKRLDALKEQKEKDQWIAQSVGIIRAIEDNFSLGVSKLDEFLKKCKSDSVKFNAERVGLTYCLKAWKRHCKKEGKISKDLSIAAQVMRRLQSLVYKYDEFLKKKDCELIVKCLKYLGFDNLLDFKDFSQEVEDDRTVKKKAKYSVDMGSARFQLLHMGHFLMRNERTDPDPRVQDFIPDTWQRELLDVVDNNESAVIVAPTSSGKTYASYYCMERVLKESDEGVAVYVAPTKALVNQVSATVQNRFTKKLPVGQALCGTFTRDYRQDVLNCQVLVTVPACLEILLLSPHRQAWVKRIRYVIFDEVHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPGHLTRWLQSVKTYWQNADRIIDGSLHSDLASRCGSKVQKKLSEAKRSYKVRLVLYGERYNHLEKYVCSFKGDDIVTDHYHPCAALTTDHIKVHGFPSDLTLSPQESLQLYDMMVSVWEKWPHAQQLDPEEFQHFKDKVAISRADAQKYEASLKKELKKWIEHGHTEKVREVLNNLKPNCVPCSQEMAVNFPLLVEKLRKMDKLPALFFIFKITDVELRANEVYKSLEVKQEEQQSPKAEKEVSNLDQRLKKVTKSIEKQPKIPPKHISKADQLLVQEAEYQQLLQKLEKLKKIPEDCTYANLKAIKNQTLYKVFHQVKFTYKSEELQTLALRGIGYHHGALNFRGRQLVEILFREGLIKVVTATGTLALGINMPCKSVVFAQNSIFLDALNYRQMSGRAGRRGQDLKGSVFFYNIPLPKIEKLIKSNVPKLRGHFPLGISLVLRLMLLASKGDDRKDAEAKVLSVLKHSLLTFSRPRAMEMLKIFFLFSLQFLVKEGYLDRECNPMGFAGLVSHLHYHEPANFLLVSFLVKGLLHKLCKPIKKGSSVFSEDVMEKLMLILANLFGRKYILAKYFEAKRKFQQSKVFLEDLPKDFEAAVFEYNVKIKNDFCTFIRIVSKLADMKQEYQLPLSKIDFTDEIYADSELLSHLMSCKEGRTAISPFVCLSGYTDRDLLHSDSLSSVVLQSVGVNDSLVPLLDLKKSDSRGRRMPLNAYALDFYKHGSITALSEDNGLHRGEVFLLLRDFLLTIQSISISLSELCEKEDDDVVLAFKQLSSSFKAKLDKVSD

Target ORF information:

RefSeq Version	XM_029076821.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus DExD/H-box helicase 60 (DDX60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029076821.1

ORF Insert Sequence:

ATGCAAGAGG ACGCTCATGC TGTCCGGGGA GGTGGTGACC AGGAAGTAAA ATTCAGAGTG 
GGGTACGAAG CTAAGAGTTT AGCATGTAAA GAGTCAGAGG AAAATGTCAC CCAAAGTCAC 
CCTGAGGATA TCAAGAAGGC TGTGGAGCCT GTGACCGATC TCCAGAAGGA GCTGCCAGAG 
AAAGGAATGA AGAAAAAAAA AGCGAAAGGG ACCCAGGCAA TAGCTGATCA TGCAGCGTCC 
ACAAAACAAA TTGGAAAAGA GAGGAGCAAG AAGACCAAAA TGAAAACAAA AGTGAAAGGA 
ACTGCAGAAG AAGAAGGTGG AAAAGGAGGA GGAGGAAGAA AGCAAAAAAC AGGAGGAGAA 
GGAGGAGGAG GAGCAGGATC AAAAGCCTGT TCTGAATTTC TGAATGAATT ATCAGAGATC 
ATTTTAAATG AAATACCAAA AGCTGAATAT TCCAGTTTGC TCGCTGACTT TGTAGAATCT 
GAATATTTTC TGTTCGATGG AGATTCTCTA CTCCTCTCCT GCATATGTGA AAAATCACTG 
GTGCTAGGAC AGAACCTTCA CTTCTTCTAT TTAGTCGAAC GCTATTTGAC GGACTTAATA 
AACAAAGGAG CCCAATTTGT CATTATTTTC TTCCAGGATA CTGAAAAAGT ATATTTCAAG 
TTTCCTCAGC TGCTTCTTCT AAGAACAACT TTAATTCTGC ACCTGAAGCA CAATACCGCC 
ATTGACGTCC GGACGGAGTT TTCCAGCTGT TTATCCCCGG AGTGGAAATA TTTCTTGGAA 
GAAAAATACC CGTATTTTTT AATAATTGCA GATAACGGCC TAAATGACCT ACAGACAAAC 
CTCTTAAACA TCTCTATCAT TCAGGCCTGG TCAATGAAAG TCAATGTTGT TCTTATCTCA 
GATCAAGCAT CGGACACAAT CCGAGTTTAT GGACACATTC TTCAGAGTAT GCACAAACAC 
CACATTTTTT TCGCAAAGAA CAGAGCAAAG ATGAAAGAAG TTTATCAGTC ACTGATGAAT 
GAAATGGAAG ATTGCAGTGA TGCTGAATTA GTCCATCTCT TTGGAGGACT GAGAAGGAAG 
ACTATAGAGC AAGAGGCATG GGAAATAGCA GAACGGTTCC AGTGTCTTTG GCCTGAAGGA 
ATCGACGTTC GACGTAACCT CTGTATCACT TCATGTTCGT TGGCTTTGAG AATGTACCAC 
AGCTACCGCT TAGGGAAGAC AGAGATGGAG GAGTCCGAAG AGTTAACCTT GGAGGAAGTA 
GCCGACCTGT GCAGGATGCA CTGCCTCAGT GTGGCTTGTC TCCTTCATCT GCCTCTCGTG 
CAAAGGGCCC AGATGCGTGT CGTCACTTCT AGATGGACTG AGCCAATAGA CTCCTTTTTG 
AACACGAAAC ACTGGTGTGA ATTTTTCGCT TTAAAAACGA TGACATTTTT GAACTTTTCC 
ACTTTAAGAA CGGATGGAAA GTACCTTCCC GACTTAAGTG ATCATCTTCT TTTGAAGAGT 
ATTGCCTATT ACTTTGAAGT CGAAAATAGC CAAGGCCTGC AATTAGAGCT CGGAAAAGCA 
ATTCAGAAGG ATTATGAGTG TCTGTGGGAT TCGATATCAG TGTTAGTTGA CCAGATTGAT 
GTTGAAAGAA CATTTCCTCT TCGAACAACG ACAAAATGTT TTCTTCAAAA GTCAAAAACT 
CCAATAAAAG AACCATCTGA TGAAAAGATG CCCAACCTGG GTTTTATTCC AGTGACATCT 
TCCGTGCTAG ACGCATTCGC TGGAGACATT CTGAAAGGTC TTTCTTTCTT AAGCAGCAAA 
GATCCAGTCG TCACTTCACT CGTGAAGCTA AAGGAATTTG ATGAACTCAA GCACTGGCAT 
TCAGGCAAAC GACTTAGTGA TGATTATGAC AGAACAAGAT GTAACCTGGA AGAAAACAGC 
AACGATCCTC ATTTTCTCAG GAGGAAGGTA AAGTTTCGGG CCTATCAACA GTTTTATGGA 
CAAACCTTGG AAGCTGTCAC TCCAAAAGTC ATTGTAACTC AACCTGCAAA GCCCACGAAA 
GGTTACGATC AAACCAAGAA CAGCAAAAGA TCCAATGAAT CAAAACAAAG CGTCATTGTC 
AGAGAAAATC AGAAAAGGTT GGATGCTCTA AAGGAGCAAA AGGAAAAGGA CCAGTGGATT 
GCCCAATCTG TAGGCATCAT AAGGGCCATT GAAGATAATT TTAGTTTGGG AGTAAGTAAA 
CTGGATGAGT TCCTGAAGAA GTGTAAGAGT GATTCGGTGA AATTTAATGC AGAAAGAGTC 
GGGCTGACTT ACTGTCTGAA AGCGTGGAAA AGACACTGCA AAAAAGAAGG TAAAATATCA 
AAAGATCTAA GTATAGCTGC CCAAGTGATG AGAAGACTTC AGTCCCTAGT GTACAAATAT 
GATGAATTTT TGAAGAAAAA AGATTGTGAA CTTATAGTCA AATGCCTTAA ATATTTAGGA 
TTTGATAATT TGTTGGATTT TAAGGATTTT TCTCAGGAGG TGGAAGATGA CAGGACTGTA 
AAGAAAAAAG CCAAGTACTC TGTTGATATG GGTTCAGCAC GATTCCAGCT CCTGCACATG 
GGCCATTTCT TGATGCGAAA TGAGAGAACA GATCCAGACC CCAGGGTCCA GGATTTCATT 
CCAGACACAT GGCAGCGAGA GCTTCTCGAC GTGGTGGATA ACAATGAGTC TGCAGTGATC 
GTTGCCCCAA CGTCTTCGGG GAAAACATAC GCCTCCTACT ACTGCATGGA GAGAGTGCTG 
AAAGAGAGCG ATGAAGGGGT GGCCGTATAT GTGGCGCCTA CGAAGGCCCT TGTGAACCAA 
GTGTCAGCAA CTGTTCAGAA TCGATTCACT AAAAAACTGC CCGTAGGTCA AGCTCTGTGT 
GGAACTTTCA CCAGGGATTA TCGCCAGGAT GTGCTGAACT GCCAGGTACT CGTGACAGTG 
CCAGCCTGTT TGGAAATCCT TCTGCTCTCT CCCCATCGTC AAGCATGGGT GAAAAGAATC 
AGATACGTTA TATTTGATGA GGTCCATTGT CTTGGTGGGG AAATTGGAGC AGAAGTTTGG 
GAGCACCTCC TTGTTATGAT CCGATGCCCT TTTTTAGCTC TCTCTGCTAC TATCAGTAAC 
CCGGGACACC TCACTAGGTG GCTCCAATCT GTCAAAACAT ATTGGCAAAA CGCAGATCGG 
ATAATAGATG GAAGTCTCCA TTCTGACCTG GCTTCTAGAT GTGGCTCCAA AGTTCAAAAA 
AAATTATCTG AAGCAAAGAG ATCATACAAA GTTAGATTGG TTCTGTACGG CGAGAGATAC 
AATCATTTGG AAAAATACGT CTGCTCCTTT AAAGGTGATG ACATCGTCAC GGATCACTAT 
CATCCATGTG CTGCCCTCAC CACAGATCAT ATTAAAGTCC ATGGATTTCC TTCAGATCTT 
ACTCTTTCTC CACAAGAAAG CCTCCAGCTT TATGACATGA TGGTTAGCGT TTGGGAGAAA 
TGGCCTCATG CCCAGCAATT AGATCCGGAA GAATTTCAAC ATTTTAAAGA TAAAGTAGCA 
ATCTCAAGGG CAGATGCCCA GAAATATGAA GCAAGTCTAA AGAAGGAACT CAAAAAATGG 
ATTGAACATG GTCATACAGA GAAGGTCAGA GAGGTACTGA ATAATTTGAA ACCTAACTGT 
GTTCCTTGTT CGCAAGAAAT GGCAGTTAAC TTCCCACTTC TTGTTGAAAA ACTAAGGAAA 
ATGGACAAAT TGCCTGCTCT GTTCTTCATT TTTAAAATAA CAGATGTGGA ACTGAGGGCT 
AATGAAGTAT ACAAGTCTCT GGAGGTGAAG CAGGAAGAAC AGCAGTCTCC CAAGGCTGAG 
AAAGAGGTCT CTAACCTAGA CCAAAGACTG AAAAAAGTGA CAAAATCCAT TGAGAAACAG 
CCCAAAATAC CTCCAAAACA CATCTCGAAA GCGGATCAAC TCCTGGTACA AGAAGCCGAG 
TATCAGCAGT TGCTCCAGAA GCTGGAGAAG CTGAAAAAGA TCCCCGAAGA CTGTACTTAC 
GCCAACCTAA AGGCTATCAA AAATCAGACT TTGTATAAAG TGTTTCACCA AGTGAAATTC 
ACATATAAAA GTGAAGAACT GCAAACTTTG GCACTGAGGG GCATTGGTTA CCATCACGGT 
GCTCTGAATT TCAGAGGAAG ACAGCTGGTT GAGATACTCT TCAGGGAGGG GTTGATCAAG 
GTAGTGACAG CTACGGGGAC GCTTGCATTA GGAATCAATA TGCCGTGCAA ATCTGTCGTT 
TTCGCTCAAA ATTCAATCTT CTTGGATGCT TTGAACTACA GACAGATGTC AGGGCGTGCA 
GGAAGGCGAG GCCAAGACCT GAAAGGAAGT GTGTTTTTCT ATAATATTCC ACTGCCCAAA 
ATAGAAAAAC TCATTAAATC CAACGTCCCT AAGCTAAGAG GCCACTTCCC GCTGGGCATA 
TCCCTGGTTC TTCGCCTCAT GCTGTTGGCT TCCAAAGGAG ATGATCGAAA GGATGCAGAA 
GCAAAGGTTT TATCAGTGCT GAAGCATTCC CTGCTGACTT TCAGCCGACC CAGAGCCATG 
GAGATGTTGA AAATATTCTT CTTGTTTTCT TTGCAGTTTC TCGTTAAAGA GGGCTATTTA 
GACCGAGAAT GTAATCCCAT GGGTTTTGCT GGACTTGTAT CACATTTGCA TTACCATGAA 
CCGGCTAATT TTCTCCTGGT CAGTTTTCTG GTGAAAGGCT TATTACACAA ACTCTGCAAA 
CCAATCAAGA AAGGCTCTTC AGTGTTTTCT GAAGATGTGA TGGAAAAGCT AATGCTGATA 
CTGGCAAACT TGTTTGGAAG GAAATACATT CTAGCCAAGT ATTTTGAAGC TAAAAGAAAA 
TTTCAGCAGT CAAAGGTGTT TCTTGAAGAT CTCCCCAAGG ATTTTGAAGC AGCCGTATTT 
GAGTACAACG TAAAAATAAA AAACGACTTT TGCACTTTTA TACGGATTGT TTCCAAACTG 
GCTGACATGA AGCAGGAGTA CCAACTCCCC TTGTCAAAAA TAGATTTCAC GGATGAAATT 
TACGCGGATT CTGAACTTTT GTCTCACTTG ATGAGCTGCA AAGAGGGGAG GACAGCCATT 
TCACCCTTCG TGTGCCTATC CGGGTACACG GATCGTGATT TACTTCACTC GGATTCTCTC 
AGCAGTGTGG TACTGCAATC AGTTGGTGTT AATGACTCCC TTGTTCCTCT CCTGGACTTA 
AAGAAATCTG ACAGTAGAGG AAGAAGAATG CCACTTAACG CATATGCACT TGACTTTTAC 
AAGCACGGAT CGATAACGGC ATTGTCAGAA GACAATGGGT TGCATAGAGG AGAAGTTTTC 
CTTCTCCTGA GAGATTTTTT GCTCACCATA CAATCTATCA GCATCTCTCT GAGTGAACTG 
TGTGAAAAAG AAGACGATGA CGTGGTCCTC GCCTTTAAAC AGCTGAGTAG TAGCTTCAAA 
GCAAAACTGG ATAAAGTCAG TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100077465 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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