ITSN2 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015285037.1	XP_015140523.1	intersectin-2 isoform X1
XM_015285040.1	XP_015140526.1	intersectin-2 isoform X4
XM_419989.6	XP_419989.4	intersectin-2 isoform X2
XM_015285035.1	XP_015140521.1	intersectin-2 isoform X1
XM_015285035.2	XP_015140521.1	intersectin-2 isoform X1
XM_015285036.1	XP_015140522.1	intersectin-2 isoform X1
XM_015285038.1	XP_015140524.1	intersectin-2 isoform X1
XM_015285039.1	XP_015140525.1	intersectin-2 isoform X3
XM_025149103.1	XP_025004871.1	intersectin-2 isoform X3
XM_015285037.2	XP_015140523.1	intersectin-2 isoform X1
XM_015285036.2	XP_015140522.1	intersectin-2 isoform X1
XM_419989.5	XP_419989.4	intersectin-2 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa148926	XM_025149103.1 Latest version!	Gallus gallus intersectin 2 (ITSN2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa34358	XM_015285040.1 Latest version!	Gallus gallus intersectin 2 (ITSN2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa06994	XM_419989.6 Latest version!	Gallus gallus intersectin 2 (ITSN2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa34356	XM_015285036.2 Latest version!	Gallus gallus intersectin 2 (ITSN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa34356	XM_015285037.2 Latest version!	Gallus gallus intersectin 2 (ITSN2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa34356	XM_015285035.2 Latest version!	Gallus gallus intersectin 2 (ITSN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa34356	XM_015285038.1 Latest version!	Gallus gallus intersectin 2 (ITSN2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa34357	XM_015285039.1 Latest version!	Gallus gallus intersectin 2 (ITSN2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OGa34356	XM_015285035.1	Gallus gallus intersectin 2 (ITSN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa34356	XM_015285037.1	Gallus gallus intersectin 2 (ITSN2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa34356	XM_015285036.1	Gallus gallus intersectin 2 (ITSN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa06994	XM_419989.5	Gallus gallus intersectin 2 (ITSN2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ITSN2 ( XM_025149103.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004871.1 Gallus gallus intersectin 2 (ITSN2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa148926
Clone ID Related Accession (Same CDS sequence)	XM_025149103.1
Accession Version	XM_025149103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004871.1
CDS	63..3866
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKCDSMDDSVLQCLLSLLSCLNNLFLLLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	XM_025149103.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149103.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_015285040.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140526.1 Gallus gallus intersectin 2 (ITSN2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa34358
Clone ID Related Accession (Same CDS sequence)	XM_015285040.1
Accession Version	XM_015285040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGATACA AGTGGACTGT ATTGTTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140526.1
CDS	294..4535
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKCDSMDDSVLQCLLSLLSCLNNLFLLLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPMERKRQGYIHELIQTEERYMDDLQLVLEVFQKPMADSGCLTEGEMGLIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGASLLQQKTDEEADFKDYLKIQVDCIVC

Target ORF information:

RefSeq Version	XM_015285040.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285040.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGATACA AGTGGACTGT ATTGTTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_419989.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_419989.4 Gallus gallus intersectin 2 (ITSN2), transcript variant X4, mRNA.

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CloneID	OGa06994
Clone ID Related Accession (Same CDS sequence)	XM_419989.6 , XM_419989.5
Accession Version	XM_419989.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_419989.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
GACCTGAGAG AAAGCTACAG CACGCAGCAA TTAGCCTTGG AGCAGCTCCA TAGGATCAAA 
CAGAGCAAAA TAAGAGAGGT AGAGAAACGA AGAGCTGAAC TTGCGCAGAA AAAGCGGCTG 
GAAGATGAGG CTACAAGGAA AGCAAAACGG GAGAAAGAGA ACCGATGGCA GGAAAATATC 
CGAAGGGAAG AGGAGGAGAA AAAGAAACGA TTAGAGGAAG AGAGAATGCA GGAAAAGGCT 
CAGGAAAAGC TCCATGAAGA GGAGGAGAAA AATAAGCAAG CCGGGATCTT GAGAGAAACA 
GAACAACGAC AGAGGCAGCT GGAGGAAGAG AAAAGAAAGC AAGAACAAAT GGCAAAAGAA 
GCTGAGGAGA GGCGTAAGCA GAATGGAGAA ATAAAGAAGA CAAAAGAAGT CACCACCTCT 
CATGAGACAG AGAAATGCAC TGCTAAATTA AACACAAGTG AAAAGTTTGG AGATCTGTTG 
AAACCAACAG AGGGTAAAAG TCTTAAGCCA CCCTGGACAA ATGAAGGCAA CACCATCAGT 
GCTTCAGACT CCAGAAGTTC CGCTGCCTTT GTAAATTACA GAGCTTTATA TCCATTTGAA 
GCAAGGAATC ATGATGAGAT GAGCTTTAAT ACTGGAGACA TAATTCAGGT TGATGAAAAA 
AATGTTGGAG AGCCTGGCTG GCTTTATGGG AGCTTCCAAG GACGTATTGG TTGGTTTCCA 
TGCAACTATG TGGAAAGAAT ACCAGAAGGA GAAAAAGCTT TATCTCCTAA GAAGGCCTTA 
CTTCCTCCCA CAGTATCTTT ATCTACTACC TCAGCTGCTT CAGAACCACT TTCACCAAGC 
AAATCTGTAG AAGAGTCGGA TTACCAGAAC GTACATTTCT CCAGCCTGAA CGTTAATGCA 
GCGTGGCAAC AGAAGTCAGC TTTTACCCGC ACTGTGTCCC CTGGATCTGT GTCACCCATT 
CATGGCCAGG GGCAGCCTGT CGAGAACTTG AAAGCACAAG CACTTTGCTC TTGGACTGCA 
AAAAAAGATA ACCACTTGAA CTTCTCAAAA AATGATATCA TTTCTGTCCT GGAGCAGCAG 
GAAAACTGGT GGTTTGGGGA GGTGCATGGA GGAAGGGGTT GGTTTCCCAA GTCGTACGTC 
AAGCTCTTAC CTGGAAGTGA GAAGAAAGAG GAACCAGAAG CTATTTATGC AGCAGTAAAG 
AAGAAGTCCA GTGCACAGCC CTACCCAGCA GGAGAGGAGT ATGTTGCACT GTATTCGTAT 
TCAAGCTCTG AGCCTGGCGA CTTGACTTTC ACCGAAGGTG AGGAAATCCT GGTGACCCAG 
AAAGAAGGGG AATGGTGGAC AGGGAGCATT GATGACAGAA CTGGGATCTT CCCCTCCAAT 
TATGTCAGAC CAAAGGATCA AGAAGGGTCT AGTAATGCTG GCAAAACTGG AACAATAAAT 
AAGAAACCTG AAATTGCTCA GGTGACTACT GCTTATGCTG CATCAGGAAG TGAACAGCTT 
AGTCTTGCTC CTGGGCAGCT CATACTTATC CTGAAGAAAA ATGCTAGTGG ATGGTGGCAA 
GGAGAGCTGC AGGCAAGAGG GAAAAAGAGA CAGAAAGGAT GGTTCCCTGC CAGCCACGTG 
AAGTTACTGG GTCCGAGCAG TGAGCGAGCC ACATCTGCTG CCCCCTCAGT GTGTCAAGTA 
ATAGCAATGT ATGATTACAT GGCAAACAAT GAAGACGAGC TCAGCTTCTC CAAAGGGCAG 
CTTATTAACG TGCTGAGCAA AGATGATGCT GACTGGTGGC AAGGAGAAAT CAATGGTGTG 
ACAGGTCTCT TCCCTTCCAA CTACGTGAAG ATGACCACAG ATTCTGATCC AAGTCAGCAG 
TGGTGTGCTG ATCTCCAAAG CCTCGATACC ATGCAGCCAA TGGAAAGGAA GAGGCAGGGT 
TACATCCACG AACTCATTCA GACGGAAGAA AGGTATATGG ATGATCTTCA GCTTGTCCTG 
GAGGTTTTCC AAAAGCCAAT GGCTGATTCG GGTTGTCTCA CAGAAGGGGA AATGGGGCTG 
ATCTTTGTTA ACTGGAAGGA ACTCATCATG TCAAATACCA AGTTACTGAA AGCCTTGCGA 
GTGCGTAAAA AAACAGGAGG AGAAAAAATG CCGGTGCAGA TGATTGGGGA CATCTTGGCA 
GCAGAGCTGT CACACATGCA GGCCTACATT CGGTTCTGCA GCTGCCAGCT TAACGGGGCT 
TCATTGCTGC AGCAGAAAAC TGATGAAGAA GCTGATTTCA AAGACTACTT AAAGAAACTC 
GCTCTGGATC CTCGCTGCAA AGGAATGCCC CTCTCCAGTT TCCTCCTGAA ACCCATGCAA 
AGAATAACAC GCTATCCTCT GCTTATAAAG AGTATTCTAG AGAATACTCC AGAAAATCAC 
CCAGATCACA GTAACCTCAA GCTTGCCTTG GAGCGTGCAG AAGAGCTGTG TTCTCAGGTT 
AATGAAGGGG TGCGTGAGAA AGAGAACTCG GACCGGCTGG AATGGATACA GTCCCACGTG 
CAGTGTGAAG GCCTTGCTGA GCAACCTGTA TTCAATTCCC TCACAAACTG CTTGGGACCG 
CGGAAGCTCC TGCACAGCGG CAAGCTCTAC AAGGCAAAGA GCAGCAAGGA GCTGTATGGC 
TTCCTGTTCA ACGACTTCCT GCTGCTTACC TACATGGTCA AACAGTTTGT CTCTTCCGGC 
TCTGATAAAC TGTTCAGCCC CAAGTCCAAC TCCCAGTTCA AAATGTACAA AATGCCTGTT 
TTCCTTAATG AAGTCCTAGT GAAATTGCCT ACAGACCCTT CAAGTGATGA GCCAGTTTTC 
CACATATCAC ACATCGACAG AGTTTACACG CTTAAAACTG ACAACATCAA CGAGCGCACT 
GCTTGGGTCC AGAAAATCAA AGCAGCATCT GAGCAGTACA TTGAAACTGA GAAGAAAAAA 
CGTGAAAAAG CATATCAAGC TCGCTCACAG AAGACATCGG GAATAGGACG TTTGATGGTG 
CACGTGATTG AAGCAACAGA GCTGAAGGCC TGTAAACCCA ATGGGAAGAG TAACCCGTAC 
TGTGAAATCA GCATGGGCTC TCAGAGCTAC ACGACGAGGA CTATGCAGGA CACCTTGAAC 
CCCAAGTGGA ACTTTAACTG CCAGTTCTTT ATTAAGGATC TGTATCAGGA TGTCCTCTGT 
ATCACCATGT TTGACAGGGA TCAATTCTCT CCTGACGATT TTTTGGGTCG CACTGAAATT 
CCGGTAGCAA AAATCCGAAC AGAACAGGAG AGCAAAGGCC CCACAACCAA GCACTTACTG 
CTGCATGAAG TGCCAACCGG TGAGGTCTGG GTCCGTTTTG ACCTGCAGCT CTTTGACCAG 
AAAACACTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_419989.4
CDS	63..5297
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPMERKRQGYIHELIQTEERYMDDLQLVLEVFQKPMADSGCLTEGEMGLIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGASLLQQKTDEEADFKDYLKKLALDPRCKGMPLSSFLLKPMQRITRYPLLIKSILENTPENHPDHSNLKLALERAEELCSQVNEGVREKENSDRLEWIQSHVQCEGLAEQPVFNSLTNCLGPRKLLHSGKLYKAKSSKELYGFLFNDFLLLTYMVKQFVSSGSDKLFSPKSNSQFKMYKMPVFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLKTDNINERTAWVQKIKAASEQYIETEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTMQDTLNPKWNFNCQFFIKDLYQDVLCITMFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPTTKHLLLHEVPTGEVWVRFDLQLFDQKTLL

Target ORF information:

RefSeq Version	XM_419989.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_419989.6

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
GACCTGAGAG AAAGCTACAG CACGCAGCAA TTAGCCTTGG AGCAGCTCCA TAGGATCAAA 
CAGAGCAAAA TAAGAGAGGT AGAGAAACGA AGAGCTGAAC TTGCGCAGAA AAAGCGGCTG 
GAAGATGAGG CTACAAGGAA AGCAAAACGG GAGAAAGAGA ACCGATGGCA GGAAAATATC 
CGAAGGGAAG AGGAGGAGAA AAAGAAACGA TTAGAGGAAG AGAGAATGCA GGAAAAGGCT 
CAGGAAAAGC TCCATGAAGA GGAGGAGAAA AATAAGCAAG CCGGGATCTT GAGAGAAACA 
GAACAACGAC AGAGGCAGCT GGAGGAAGAG AAAAGAAAGC AAGAACAAAT GGCAAAAGAA 
GCTGAGGAGA GGCGTAAGCA GAATGGAGAA ATAAAGAAGA CAAAAGAAGT CACCACCTCT 
CATGAGACAG AGAAATGCAC TGCTAAATTA AACACAAGTG AAAAGTTTGG AGATCTGTTG 
AAACCAACAG AGGGTAAAAG TCTTAAGCCA CCCTGGACAA ATGAAGGCAA CACCATCAGT 
GCTTCAGACT CCAGAAGTTC CGCTGCCTTT GTAAATTACA GAGCTTTATA TCCATTTGAA 
GCAAGGAATC ATGATGAGAT GAGCTTTAAT ACTGGAGACA TAATTCAGGT TGATGAAAAA 
AATGTTGGAG AGCCTGGCTG GCTTTATGGG AGCTTCCAAG GACGTATTGG TTGGTTTCCA 
TGCAACTATG TGGAAAGAAT ACCAGAAGGA GAAAAAGCTT TATCTCCTAA GAAGGCCTTA 
CTTCCTCCCA CAGTATCTTT ATCTACTACC TCAGCTGCTT CAGAACCACT TTCACCAAGC 
AAATCTGTAG AAGAGTCGGA TTACCAGAAC GTACATTTCT CCAGCCTGAA CGTTAATGCA 
GCGTGGCAAC AGAAGTCAGC TTTTACCCGC ACTGTGTCCC CTGGATCTGT GTCACCCATT 
CATGGCCAGG GGCAGCCTGT CGAGAACTTG AAAGCACAAG CACTTTGCTC TTGGACTGCA 
AAAAAAGATA ACCACTTGAA CTTCTCAAAA AATGATATCA TTTCTGTCCT GGAGCAGCAG 
GAAAACTGGT GGTTTGGGGA GGTGCATGGA GGAAGGGGTT GGTTTCCCAA GTCGTACGTC 
AAGCTCTTAC CTGGAAGTGA GAAGAAAGAG GAACCAGAAG CTATTTATGC AGCAGTAAAG 
AAGAAGTCCA GTGCACAGCC CTACCCAGCA GGAGAGGAGT ATGTTGCACT GTATTCGTAT 
TCAAGCTCTG AGCCTGGCGA CTTGACTTTC ACCGAAGGTG AGGAAATCCT GGTGACCCAG 
AAAGAAGGGG AATGGTGGAC AGGGAGCATT GATGACAGAA CTGGGATCTT CCCCTCCAAT 
TATGTCAGAC CAAAGGATCA AGAAGGGTCT AGTAATGCTG GCAAAACTGG AACAATAAAT 
AAGAAACCTG AAATTGCTCA GGTGACTACT GCTTATGCTG CATCAGGAAG TGAACAGCTT 
AGTCTTGCTC CTGGGCAGCT CATACTTATC CTGAAGAAAA ATGCTAGTGG ATGGTGGCAA 
GGAGAGCTGC AGGCAAGAGG GAAAAAGAGA CAGAAAGGAT GGTTCCCTGC CAGCCACGTG 
AAGTTACTGG GTCCGAGCAG TGAGCGAGCC ACATCTGCTG CCCCCTCAGT GTGTCAAGTA 
ATAGCAATGT ATGATTACAT GGCAAACAAT GAAGACGAGC TCAGCTTCTC CAAAGGGCAG 
CTTATTAACG TGCTGAGCAA AGATGATGCT GACTGGTGGC AAGGAGAAAT CAATGGTGTG 
ACAGGTCTCT TCCCTTCCAA CTACGTGAAG ATGACCACAG ATTCTGATCC AAGTCAGCAG 
TGGTGTGCTG ATCTCCAAAG CCTCGATACC ATGCAGCCAA TGGAAAGGAA GAGGCAGGGT 
TACATCCACG AACTCATTCA GACGGAAGAA AGGTATATGG ATGATCTTCA GCTTGTCCTG 
GAGGTTTTCC AAAAGCCAAT GGCTGATTCG GGTTGTCTCA CAGAAGGGGA AATGGGGCTG 
ATCTTTGTTA ACTGGAAGGA ACTCATCATG TCAAATACCA AGTTACTGAA AGCCTTGCGA 
GTGCGTAAAA AAACAGGAGG AGAAAAAATG CCGGTGCAGA TGATTGGGGA CATCTTGGCA 
GCAGAGCTGT CACACATGCA GGCCTACATT CGGTTCTGCA GCTGCCAGCT TAACGGGGCT 
TCATTGCTGC AGCAGAAAAC TGATGAAGAA GCTGATTTCA AAGACTACTT AAAGAAACTC 
GCTCTGGATC CTCGCTGCAA AGGAATGCCC CTCTCCAGTT TCCTCCTGAA ACCCATGCAA 
AGAATAACAC GCTATCCTCT GCTTATAAAG AGTATTCTAG AGAATACTCC AGAAAATCAC 
CCAGATCACA GTAACCTCAA GCTTGCCTTG GAGCGTGCAG AAGAGCTGTG TTCTCAGGTT 
AATGAAGGGG TGCGTGAGAA AGAGAACTCG GACCGGCTGG AATGGATACA GTCCCACGTG 
CAGTGTGAAG GCCTTGCTGA GCAACCTGTA TTCAATTCCC TCACAAACTG CTTGGGACCG 
CGGAAGCTCC TGCACAGCGG CAAGCTCTAC AAGGCAAAGA GCAGCAAGGA GCTGTATGGC 
TTCCTGTTCA ACGACTTCCT GCTGCTTACC TACATGGTCA AACAGTTTGT CTCTTCCGGC 
TCTGATAAAC TGTTCAGCCC CAAGTCCAAC TCCCAGTTCA AAATGTACAA AATGCCTGTT 
TTCCTTAATG AAGTCCTAGT GAAATTGCCT ACAGACCCTT CAAGTGATGA GCCAGTTTTC 
CACATATCAC ACATCGACAG AGTTTACACG CTTAAAACTG ACAACATCAA CGAGCGCACT 
GCTTGGGTCC AGAAAATCAA AGCAGCATCT GAGCAGTACA TTGAAACTGA GAAGAAAAAA 
CGTGAAAAAG CATATCAAGC TCGCTCACAG AAGACATCGG GAATAGGACG TTTGATGGTG 
CACGTGATTG AAGCAACAGA GCTGAAGGCC TGTAAACCCA ATGGGAAGAG TAACCCGTAC 
TGTGAAATCA GCATGGGCTC TCAGAGCTAC ACGACGAGGA CTATGCAGGA CACCTTGAAC 
CCCAAGTGGA ACTTTAACTG CCAGTTCTTT ATTAAGGATC TGTATCAGGA TGTCCTCTGT 
ATCACCATGT TTGACAGGGA TCAATTCTCT CCTGACGATT TTTTGGGTCG CACTGAAATT 
CCGGTAGCAA AAATCCGAAC AGAACAGGAG AGCAAAGGCC CCACAACCAA GCACTTACTG 
CTGCATGAAG TGCCAACCGG TGAGGTCTGG GTCCGTTTTG ACCTGCAGCT CTTTGACCAG 
AAAACACTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_419989.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_419989.4 Gallus gallus intersectin 2 (ITSN2), transcript variant X5, mRNA.

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CloneID	OGa06994
Clone ID Related Accession (Same CDS sequence)	XM_419989.6 , XM_419989.5
Accession Version	XM_419989.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455846.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_419989.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
GACCTGAGAG AAAGCTACAG CACGCAGCAA TTAGCCTTGG AGCAGCTCCA TAGGATCAAA 
CAGAGCAAAA TAAGAGAGGT AGAGAAACGA AGAGCTGAAC TTGCGCAGAA AAAGCGGCTG 
GAAGATGAGG CTACAAGGAA AGCAAAACGG GAGAAAGAGA ACCGATGGCA GGAAAATATC 
CGAAGGGAAG AGGAGGAGAA AAAGAAACGA TTAGAGGAAG AGAGAATGCA GGAAAAGGCT 
CAGGAAAAGC TCCATGAAGA GGAGGAGAAA AATAAGCAAG CCGGGATCTT GAGAGAAACA 
GAACAACGAC AGAGGCAGCT GGAGGAAGAG AAAAGAAAGC AAGAACAAAT GGCAAAAGAA 
GCTGAGGAGA GGCGTAAGCA GAATGGAGAA ATAAAGAAGA CAAAAGAAGT CACCACCTCT 
CATGAGACAG AGAAATGCAC TGCTAAATTA AACACAAGTG AAAAGTTTGG AGATCTGTTG 
AAACCAACAG AGGGTAAAAG TCTTAAGCCA CCCTGGACAA ATGAAGGCAA CACCATCAGT 
GCTTCAGACT CCAGAAGTTC CGCTGCCTTT GTAAATTACA GAGCTTTATA TCCATTTGAA 
GCAAGGAATC ATGATGAGAT GAGCTTTAAT ACTGGAGACA TAATTCAGGT TGATGAAAAA 
AATGTTGGAG AGCCTGGCTG GCTTTATGGG AGCTTCCAAG GACGTATTGG TTGGTTTCCA 
TGCAACTATG TGGAAAGAAT ACCAGAAGGA GAAAAAGCTT TATCTCCTAA GAAGGCCTTA 
CTTCCTCCCA CAGTATCTTT ATCTACTACC TCAGCTGCTT CAGAACCACT TTCACCAAGC 
AAATCTGTAG AAGAGTCGGA TTACCAGAAC GTACATTTCT CCAGCCTGAA CGTTAATGCA 
GCGTGGCAAC AGAAGTCAGC TTTTACCCGC ACTGTGTCCC CTGGATCTGT GTCACCCATT 
CATGGCCAGG GGCAGCCTGT CGAGAACTTG AAAGCACAAG CACTTTGCTC TTGGACTGCA 
AAAAAAGATA ACCACTTGAA CTTCTCAAAA AATGATATCA TTTCTGTCCT GGAGCAGCAG 
GAAAACTGGT GGTTTGGGGA GGTGCATGGA GGAAGGGGTT GGTTTCCCAA GTCGTACGTC 
AAGCTCTTAC CTGGAAGTGA GAAGAAAGAG GAACCAGAAG CTATTTATGC AGCAGTAAAG 
AAGAAGTCCA GTGCACAGCC CTACCCAGCA GGAGAGGAGT ATGTTGCACT GTATTCGTAT 
TCAAGCTCTG AGCCTGGCGA CTTGACTTTC ACCGAAGGTG AGGAAATCCT GGTGACCCAG 
AAAGAAGGGG AATGGTGGAC AGGGAGCATT GATGACAGAA CTGGGATCTT CCCCTCCAAT 
TATGTCAGAC CAAAGGATCA AGAAGGGTCT AGTAATGCTG GCAAAACTGG AACAATAAAT 
AAGAAACCTG AAATTGCTCA GGTGACTACT GCTTATGCTG CATCAGGAAG TGAACAGCTT 
AGTCTTGCTC CTGGGCAGCT CATACTTATC CTGAAGAAAA ATGCTAGTGG ATGGTGGCAA 
GGAGAGCTGC AGGCAAGAGG GAAAAAGAGA CAGAAAGGAT GGTTCCCTGC CAGCCACGTG 
AAGTTACTGG GTCCGAGCAG TGAGCGAGCC ACATCTGCTG CCCCCTCAGT GTGTCAAGTA 
ATAGCAATGT ATGATTACAT GGCAAACAAT GAAGACGAGC TCAGCTTCTC CAAAGGGCAG 
CTTATTAACG TGCTGAGCAA AGATGATGCT GACTGGTGGC AAGGAGAAAT CAATGGTGTG 
ACAGGTCTCT TCCCTTCCAA CTACGTGAAG ATGACCACAG ATTCTGATCC AAGTCAGCAG 
TGGTGTGCTG ATCTCCAAAG CCTCGATACC ATGCAGCCAA TGGAAAGGAA GAGGCAGGGT 
TACATCCACG AACTCATTCA GACGGAAGAA AGGTATATGG ATGATCTTCA GCTTGTCCTG 
GAGGTTTTCC AAAAGCCAAT GGCTGATTCG GGTTGTCTCA CAGAAGGGGA AATGGGGCTG 
ATCTTTGTTA ACTGGAAGGA ACTCATCATG TCAAATACCA AGTTACTGAA AGCCTTGCGA 
GTGCGTAAAA AAACAGGAGG AGAAAAAATG CCGGTGCAGA TGATTGGGGA CATCTTGGCA 
GCAGAGCTGT CACACATGCA GGCCTACATT CGGTTCTGCA GCTGCCAGCT TAACGGGGCT 
TCATTGCTGC AGCAGAAAAC TGATGAAGAA GCTGATTTCA AAGACTACTT AAAGAAACTC 
GCTCTGGATC CTCGCTGCAA AGGAATGCCC CTCTCCAGTT TCCTCCTGAA ACCCATGCAA 
AGAATAACAC GCTATCCTCT GCTTATAAAG AGTATTCTAG AGAATACTCC AGAAAATCAC 
CCAGATCACA GTAACCTCAA GCTTGCCTTG GAGCGTGCAG AAGAGCTGTG TTCTCAGGTT 
AATGAAGGGG TGCGTGAGAA AGAGAACTCG GACCGGCTGG AATGGATACA GTCCCACGTG 
CAGTGTGAAG GCCTTGCTGA GCAACCTGTA TTCAATTCCC TCACAAACTG CTTGGGACCG 
CGGAAGCTCC TGCACAGCGG CAAGCTCTAC AAGGCAAAGA GCAGCAAGGA GCTGTATGGC 
TTCCTGTTCA ACGACTTCCT GCTGCTTACC TACATGGTCA AACAGTTTGT CTCTTCCGGC 
TCTGATAAAC TGTTCAGCCC CAAGTCCAAC TCCCAGTTCA AAATGTACAA AATGCCTGTT 
TTCCTTAATG AAGTCCTAGT GAAATTGCCT ACAGACCCTT CAAGTGATGA GCCAGTTTTC 
CACATATCAC ACATCGACAG AGTTTACACG CTTAAAACTG ACAACATCAA CGAGCGCACT 
GCTTGGGTCC AGAAAATCAA AGCAGCATCT GAGCAGTACA TTGAAACTGA GAAGAAAAAA 
CGTGAAAAAG CATATCAAGC TCGCTCACAG AAGACATCGG GAATAGGACG TTTGATGGTG 
CACGTGATTG AAGCAACAGA GCTGAAGGCC TGTAAACCCA ATGGGAAGAG TAACCCGTAC 
TGTGAAATCA GCATGGGCTC TCAGAGCTAC ACGACGAGGA CTATGCAGGA CACCTTGAAC 
CCCAAGTGGA ACTTTAACTG CCAGTTCTTT ATTAAGGATC TGTATCAGGA TGTCCTCTGT 
ATCACCATGT TTGACAGGGA TCAATTCTCT CCTGACGATT TTTTGGGTCG CACTGAAATT 
CCGGTAGCAA AAATCCGAAC AGAACAGGAG AGCAAAGGCC CCACAACCAA GCACTTACTG 
CTGCATGAAG TGCCAACCGG TGAGGTCTGG GTCCGTTTTG ACCTGCAGCT CTTTGACCAG 
AAAACACTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_419989.4
CDS	294..5528
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPMERKRQGYIHELIQTEERYMDDLQLVLEVFQKPMADSGCLTEGEMGLIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGASLLQQKTDEEADFKDYLKKLALDPRCKGMPLSSFLLKPMQRITRYPLLIKSILENTPENHPDHSNLKLALERAEELCSQVNEGVREKENSDRLEWIQSHVQCEGLAEQPVFNSLTNCLGPRKLLHSGKLYKAKSSKELYGFLFNDFLLLTYMVKQFVSSGSDKLFSPKSNSQFKMYKMPVFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLKTDNINERTAWVQKIKAASEQYIETEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTMQDTLNPKWNFNCQFFIKDLYQDVLCITMFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPTTKHLLLHEVPTGEVWVRFDLQLFDQKTLL

Target ORF information:

RefSeq Version	XM_419989.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_419989.5

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
GACCTGAGAG AAAGCTACAG CACGCAGCAA TTAGCCTTGG AGCAGCTCCA TAGGATCAAA 
CAGAGCAAAA TAAGAGAGGT AGAGAAACGA AGAGCTGAAC TTGCGCAGAA AAAGCGGCTG 
GAAGATGAGG CTACAAGGAA AGCAAAACGG GAGAAAGAGA ACCGATGGCA GGAAAATATC 
CGAAGGGAAG AGGAGGAGAA AAAGAAACGA TTAGAGGAAG AGAGAATGCA GGAAAAGGCT 
CAGGAAAAGC TCCATGAAGA GGAGGAGAAA AATAAGCAAG CCGGGATCTT GAGAGAAACA 
GAACAACGAC AGAGGCAGCT GGAGGAAGAG AAAAGAAAGC AAGAACAAAT GGCAAAAGAA 
GCTGAGGAGA GGCGTAAGCA GAATGGAGAA ATAAAGAAGA CAAAAGAAGT CACCACCTCT 
CATGAGACAG AGAAATGCAC TGCTAAATTA AACACAAGTG AAAAGTTTGG AGATCTGTTG 
AAACCAACAG AGGGTAAAAG TCTTAAGCCA CCCTGGACAA ATGAAGGCAA CACCATCAGT 
GCTTCAGACT CCAGAAGTTC CGCTGCCTTT GTAAATTACA GAGCTTTATA TCCATTTGAA 
GCAAGGAATC ATGATGAGAT GAGCTTTAAT ACTGGAGACA TAATTCAGGT TGATGAAAAA 
AATGTTGGAG AGCCTGGCTG GCTTTATGGG AGCTTCCAAG GACGTATTGG TTGGTTTCCA 
TGCAACTATG TGGAAAGAAT ACCAGAAGGA GAAAAAGCTT TATCTCCTAA GAAGGCCTTA 
CTTCCTCCCA CAGTATCTTT ATCTACTACC TCAGCTGCTT CAGAACCACT TTCACCAAGC 
AAATCTGTAG AAGAGTCGGA TTACCAGAAC GTACATTTCT CCAGCCTGAA CGTTAATGCA 
GCGTGGCAAC AGAAGTCAGC TTTTACCCGC ACTGTGTCCC CTGGATCTGT GTCACCCATT 
CATGGCCAGG GGCAGCCTGT CGAGAACTTG AAAGCACAAG CACTTTGCTC TTGGACTGCA 
AAAAAAGATA ACCACTTGAA CTTCTCAAAA AATGATATCA TTTCTGTCCT GGAGCAGCAG 
GAAAACTGGT GGTTTGGGGA GGTGCATGGA GGAAGGGGTT GGTTTCCCAA GTCGTACGTC 
AAGCTCTTAC CTGGAAGTGA GAAGAAAGAG GAACCAGAAG CTATTTATGC AGCAGTAAAG 
AAGAAGTCCA GTGCACAGCC CTACCCAGCA GGAGAGGAGT ATGTTGCACT GTATTCGTAT 
TCAAGCTCTG AGCCTGGCGA CTTGACTTTC ACCGAAGGTG AGGAAATCCT GGTGACCCAG 
AAAGAAGGGG AATGGTGGAC AGGGAGCATT GATGACAGAA CTGGGATCTT CCCCTCCAAT 
TATGTCAGAC CAAAGGATCA AGAAGGGTCT AGTAATGCTG GCAAAACTGG AACAATAAAT 
AAGAAACCTG AAATTGCTCA GGTGACTACT GCTTATGCTG CATCAGGAAG TGAACAGCTT 
AGTCTTGCTC CTGGGCAGCT CATACTTATC CTGAAGAAAA ATGCTAGTGG ATGGTGGCAA 
GGAGAGCTGC AGGCAAGAGG GAAAAAGAGA CAGAAAGGAT GGTTCCCTGC CAGCCACGTG 
AAGTTACTGG GTCCGAGCAG TGAGCGAGCC ACATCTGCTG CCCCCTCAGT GTGTCAAGTA 
ATAGCAATGT ATGATTACAT GGCAAACAAT GAAGACGAGC TCAGCTTCTC CAAAGGGCAG 
CTTATTAACG TGCTGAGCAA AGATGATGCT GACTGGTGGC AAGGAGAAAT CAATGGTGTG 
ACAGGTCTCT TCCCTTCCAA CTACGTGAAG ATGACCACAG ATTCTGATCC AAGTCAGCAG 
TGGTGTGCTG ATCTCCAAAG CCTCGATACC ATGCAGCCAA TGGAAAGGAA GAGGCAGGGT 
TACATCCACG AACTCATTCA GACGGAAGAA AGGTATATGG ATGATCTTCA GCTTGTCCTG 
GAGGTTTTCC AAAAGCCAAT GGCTGATTCG GGTTGTCTCA CAGAAGGGGA AATGGGGCTG 
ATCTTTGTTA ACTGGAAGGA ACTCATCATG TCAAATACCA AGTTACTGAA AGCCTTGCGA 
GTGCGTAAAA AAACAGGAGG AGAAAAAATG CCGGTGCAGA TGATTGGGGA CATCTTGGCA 
GCAGAGCTGT CACACATGCA GGCCTACATT CGGTTCTGCA GCTGCCAGCT TAACGGGGCT 
TCATTGCTGC AGCAGAAAAC TGATGAAGAA GCTGATTTCA AAGACTACTT AAAGAAACTC 
GCTCTGGATC CTCGCTGCAA AGGAATGCCC CTCTCCAGTT TCCTCCTGAA ACCCATGCAA 
AGAATAACAC GCTATCCTCT GCTTATAAAG AGTATTCTAG AGAATACTCC AGAAAATCAC 
CCAGATCACA GTAACCTCAA GCTTGCCTTG GAGCGTGCAG AAGAGCTGTG TTCTCAGGTT 
AATGAAGGGG TGCGTGAGAA AGAGAACTCG GACCGGCTGG AATGGATACA GTCCCACGTG 
CAGTGTGAAG GCCTTGCTGA GCAACCTGTA TTCAATTCCC TCACAAACTG CTTGGGACCG 
CGGAAGCTCC TGCACAGCGG CAAGCTCTAC AAGGCAAAGA GCAGCAAGGA GCTGTATGGC 
TTCCTGTTCA ACGACTTCCT GCTGCTTACC TACATGGTCA AACAGTTTGT CTCTTCCGGC 
TCTGATAAAC TGTTCAGCCC CAAGTCCAAC TCCCAGTTCA AAATGTACAA AATGCCTGTT 
TTCCTTAATG AAGTCCTAGT GAAATTGCCT ACAGACCCTT CAAGTGATGA GCCAGTTTTC 
CACATATCAC ACATCGACAG AGTTTACACG CTTAAAACTG ACAACATCAA CGAGCGCACT 
GCTTGGGTCC AGAAAATCAA AGCAGCATCT GAGCAGTACA TTGAAACTGA GAAGAAAAAA 
CGTGAAAAAG CATATCAAGC TCGCTCACAG AAGACATCGG GAATAGGACG TTTGATGGTG 
CACGTGATTG AAGCAACAGA GCTGAAGGCC TGTAAACCCA ATGGGAAGAG TAACCCGTAC 
TGTGAAATCA GCATGGGCTC TCAGAGCTAC ACGACGAGGA CTATGCAGGA CACCTTGAAC 
CCCAAGTGGA ACTTTAACTG CCAGTTCTTT ATTAAGGATC TGTATCAGGA TGTCCTCTGT 
ATCACCATGT TTGACAGGGA TCAATTCTCT CCTGACGATT TTTTGGGTCG CACTGAAATT 
CCGGTAGCAA AAATCCGAAC AGAACAGGAG AGCAAAGGCC CCACAACCAA GCACTTACTG 
CTGCATGAAG TGCCAACCGG TGAGGTCTGG GTCCGTTTTG ACCTGCAGCT CTTTGACCAG 
AAAACACTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_015285037.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140523.1 Gallus gallus intersectin 2 (ITSN2), transcript variant X3, mRNA.

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CloneID	OGa34356
Clone ID Related Accession (Same CDS sequence)	XM_015285037.1 , XM_015285035.1 , XM_015285035.2 , XM_015285036.1 , XM_015285038.1 , XM_015285037.2 , XM_015285036.2
Accession Version	XM_015285037.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140523.1
CDS	59..5374
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKCDSMDDSVLQCLLSLLSCLNNLFLLLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPMERKRQGYIHELIQTEERYMDDLQLVLEVFQKPMADSGCLTEGEMGLIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGASLLQQKTDEEADFKDYLKKLALDPRCKGMPLSSFLLKPMQRITRYPLLIKSILENTPENHPDHSNLKLALERAEELCSQVNEGVREKENSDRLEWIQSHVQCEGLAEQPVFNSLTNCLGPRKLLHSGKLYKAKSSKELYGFLFNDFLLLTYMVKQFVSSGSDKLFSPKSNSQFKMYKMPVFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLKTDNINERTAWVQKIKAASEQYIETEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTMQDTLNPKWNFNCQFFIKDLYQDVLCITMFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPTTKHLLLHEVPTGEVWVRFDLQLFDQKTLL

Target ORF information:

RefSeq Version	XM_015285037.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285037.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_015285035.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140521.1 Gallus gallus intersectin 2 (ITSN2), transcript variant X1, mRNA.

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CloneID	OGa34356
Clone ID Related Accession (Same CDS sequence)	XM_015285037.1 , XM_015285035.1 , XM_015285035.2 , XM_015285036.1 , XM_015285038.1 , XM_015285037.2 , XM_015285036.2
Accession Version	XM_015285035.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140521.1
CDS	295..5610
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKCDSMDDSVLQCLLSLLSCLNNLFLLLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPMERKRQGYIHELIQTEERYMDDLQLVLEVFQKPMADSGCLTEGEMGLIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGASLLQQKTDEEADFKDYLKKLALDPRCKGMPLSSFLLKPMQRITRYPLLIKSILENTPENHPDHSNLKLALERAEELCSQVNEGVREKENSDRLEWIQSHVQCEGLAEQPVFNSLTNCLGPRKLLHSGKLYKAKSSKELYGFLFNDFLLLTYMVKQFVSSGSDKLFSPKSNSQFKMYKMPVFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLKTDNINERTAWVQKIKAASEQYIETEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTMQDTLNPKWNFNCQFFIKDLYQDVLCITMFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPTTKHLLLHEVPTGEVWVRFDLQLFDQKTLL

Target ORF information:

RefSeq Version	XM_015285035.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285035.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_015285035.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140521.1 Gallus gallus intersectin 2 (ITSN2), transcript variant X1, mRNA.

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CloneID	OGa34356
Clone ID Related Accession (Same CDS sequence)	XM_015285037.1 , XM_015285035.1 , XM_015285035.2 , XM_015285036.1 , XM_015285038.1 , XM_015285037.2 , XM_015285036.2
Accession Version	XM_015285035.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285035.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140521.1
CDS	63..5378
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKCDSMDDSVLQCLLSLLSCLNNLFLLLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPMERKRQGYIHELIQTEERYMDDLQLVLEVFQKPMADSGCLTEGEMGLIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGASLLQQKTDEEADFKDYLKKLALDPRCKGMPLSSFLLKPMQRITRYPLLIKSILENTPENHPDHSNLKLALERAEELCSQVNEGVREKENSDRLEWIQSHVQCEGLAEQPVFNSLTNCLGPRKLLHSGKLYKAKSSKELYGFLFNDFLLLTYMVKQFVSSGSDKLFSPKSNSQFKMYKMPVFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLKTDNINERTAWVQKIKAASEQYIETEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTMQDTLNPKWNFNCQFFIKDLYQDVLCITMFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPTTKHLLLHEVPTGEVWVRFDLQLFDQKTLL

Target ORF information:

RefSeq Version	XM_015285035.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285035.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_015285036.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140522.1 Gallus gallus intersectin 2 (ITSN2), transcript variant X2, mRNA.

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CloneID	OGa34356
Clone ID Related Accession (Same CDS sequence)	XM_015285037.1 , XM_015285035.1 , XM_015285035.2 , XM_015285036.1 , XM_015285038.1 , XM_015285037.2 , XM_015285036.2
Accession Version	XM_015285036.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140522.1
CDS	62..5377
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKCDSMDDSVLQCLLSLLSCLNNLFLLLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPMERKRQGYIHELIQTEERYMDDLQLVLEVFQKPMADSGCLTEGEMGLIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGASLLQQKTDEEADFKDYLKKLALDPRCKGMPLSSFLLKPMQRITRYPLLIKSILENTPENHPDHSNLKLALERAEELCSQVNEGVREKENSDRLEWIQSHVQCEGLAEQPVFNSLTNCLGPRKLLHSGKLYKAKSSKELYGFLFNDFLLLTYMVKQFVSSGSDKLFSPKSNSQFKMYKMPVFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLKTDNINERTAWVQKIKAASEQYIETEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTMQDTLNPKWNFNCQFFIKDLYQDVLCITMFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPTTKHLLLHEVPTGEVWVRFDLQLFDQKTLL

Target ORF information:

RefSeq Version	XM_015285036.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285036.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_015285038.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140524.1 Gallus gallus intersectin 2 (ITSN2), transcript variant X4, mRNA.

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CloneID	OGa34356
Clone ID Related Accession (Same CDS sequence)	XM_015285037.1 , XM_015285035.1 , XM_015285035.2 , XM_015285036.1 , XM_015285038.1 , XM_015285037.2 , XM_015285036.2
Accession Version	XM_015285038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140524.1
CDS	53..5368
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKCDSMDDSVLQCLLSLLSCLNNLFLLLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPMERKRQGYIHELIQTEERYMDDLQLVLEVFQKPMADSGCLTEGEMGLIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGASLLQQKTDEEADFKDYLKKLALDPRCKGMPLSSFLLKPMQRITRYPLLIKSILENTPENHPDHSNLKLALERAEELCSQVNEGVREKENSDRLEWIQSHVQCEGLAEQPVFNSLTNCLGPRKLLHSGKLYKAKSSKELYGFLFNDFLLLTYMVKQFVSSGSDKLFSPKSNSQFKMYKMPVFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLKTDNINERTAWVQKIKAASEQYIETEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTMQDTLNPKWNFNCQFFIKDLYQDVLCITMFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPTTKHLLLHEVPTGEVWVRFDLQLFDQKTLL

Target ORF information:

RefSeq Version	XM_015285038.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285038.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_015285037.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140523.1 Gallus gallus intersectin 2 (ITSN2), transcript variant X3, mRNA.

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CloneID	OGa34356
Clone ID Related Accession (Same CDS sequence)	XM_015285037.1 , XM_015285035.1 , XM_015285035.2 , XM_015285036.1 , XM_015285038.1 , XM_015285037.2 , XM_015285036.2
Accession Version	XM_015285037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140523.1
CDS	53..5368
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKCDSMDDSVLQCLLSLLSCLNNLFLLLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPMERKRQGYIHELIQTEERYMDDLQLVLEVFQKPMADSGCLTEGEMGLIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGASLLQQKTDEEADFKDYLKKLALDPRCKGMPLSSFLLKPMQRITRYPLLIKSILENTPENHPDHSNLKLALERAEELCSQVNEGVREKENSDRLEWIQSHVQCEGLAEQPVFNSLTNCLGPRKLLHSGKLYKAKSSKELYGFLFNDFLLLTYMVKQFVSSGSDKLFSPKSNSQFKMYKMPVFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLKTDNINERTAWVQKIKAASEQYIETEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTMQDTLNPKWNFNCQFFIKDLYQDVLCITMFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPTTKHLLLHEVPTGEVWVRFDLQLFDQKTLL

Target ORF information:

RefSeq Version	XM_015285037.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285037.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_015285036.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140522.1 Gallus gallus intersectin 2 (ITSN2), transcript variant X2, mRNA.

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CloneID	OGa34356
Clone ID Related Accession (Same CDS sequence)	XM_015285037.1 , XM_015285035.1 , XM_015285035.2 , XM_015285036.1 , XM_015285038.1 , XM_015285037.2 , XM_015285036.2
Accession Version	XM_015285036.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285036.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140522.1
CDS	57..5372
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKCDSMDDSVLQCLLSLLSCLNNLFLLLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPMERKRQGYIHELIQTEERYMDDLQLVLEVFQKPMADSGCLTEGEMGLIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGASLLQQKTDEEADFKDYLKKLALDPRCKGMPLSSFLLKPMQRITRYPLLIKSILENTPENHPDHSNLKLALERAEELCSQVNEGVREKENSDRLEWIQSHVQCEGLAEQPVFNSLTNCLGPRKLLHSGKLYKAKSSKELYGFLFNDFLLLTYMVKQFVSSGSDKLFSPKSNSQFKMYKMPVFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLKTDNINERTAWVQKIKAASEQYIETEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTMQDTLNPKWNFNCQFFIKDLYQDVLCITMFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPTTKHLLLHEVPTGEVWVRFDLQLFDQKTLL

Target ORF information:

RefSeq Version	XM_015285036.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285036.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGCAC TGCTTGGGTC CAGAAAATCA AAGCAGCATC TGAGCAGTAC 
ATTGAAACTG AGAAGAAAAA ACGTGAAAAA GCATATCAAG CTCGCTCACA GAAGACATCG 
GGAATAGGAC GTTTGATGGT GCACGTGATT GAAGCAACAG AGCTGAAGGC CTGTAAACCC 
AATGGGAAGA GTAACCCGTA CTGTGAAATC AGCATGGGCT CTCAGAGCTA CACGACGAGG 
ACTATGCAGG ACACCTTGAA CCCCAAGTGG AACTTTAACT GCCAGTTCTT TATTAAGGAT 
CTGTATCAGG ATGTCCTCTG TATCACCATG TTTGACAGGG ATCAATTCTC TCCTGACGAT 
TTTTTGGGTC GCACTGAAAT TCCGGTAGCA AAAATCCGAA CAGAACAGGA GAGCAAAGGC 
CCCACAACCA AGCACTTACT GCTGCATGAA GTGCCAACCG GTGAGGTCTG GGTCCGTTTT 
GACCTGCAGC TCTTTGACCA GAAAACACTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_015285039.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140525.1 Gallus gallus intersectin 2 (ITSN2), transcript variant X6, mRNA.

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CloneID	OGa34357
Clone ID Related Accession (Same CDS sequence)	XM_015285039.1
Accession Version	XM_015285039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	intersectin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGAGC AGAACTCTTG CAGTTGGATA TAGCATTGCA TCTACTGCTC 
TCAGATGTCG TAGCTGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140525.1
CDS	295..5175
Translation	MMAQFPTAVNGGPNIWAITAEERAKHDKQFDSLKPTGGYITGDQARTFFLQSGLPSSVLADIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQHLPMVLPPVMKQTPVLSPLMSARFGMGSMPNLSMPTSMSTITPLAPMSLTSMTSIPPLVISAPLVPSVSTSSLPNGTSSLLQPLAIPFSSTQPHTGSLGPVAGGFGGSNMQKAQSLIDLGSSSSNSSSSASLAGNSPKIAPSDWAVPQASRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWSLADIDGDGQLKADEFVLAMHLTDMAKAGQPLPLTLPLELVPPSFRGGKLTENINGTLPSYQPVQEEEPQKKQPVTFEDKRKANYERGNVELEKRRQVLLEQQQREAERKAQKEREEQERRERERQEQERKKQLELERQLERQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIIKLTSKKKSLNLELEALTDKHQQISGRLQDIRSKKQIRRTELEALDRKCDQGIMEVKQLQQQLQEYQNKLTYLVPEKQLLTERIKNVQLDSTQNAGIASVQKKSLEKEELCQRLKEQLDALEKETASKLAEMDEFNSQLKCDSMDDSVLQCLLSLLSCLNNLFLLLKDLRESYSTQQLALEQLHRIKQSKIREVEKRRAELAQKKRLEDEATRKAKREKENRWQENIRREEEEKKKRLEEERMQEKAQEKLHEEEEKNKQAGILRETEQRQRQLEEEKRKQEQMAKEAEERRKQNGEIKKTKEVTTSHETEKCTAKLNTSEKFGDLLKPTEGKSLKPPWTNEGNTISASDSRSSAAFVNYRALYPFEARNHDEMSFNTGDIIQVDEKNVGEPGWLYGSFQGRIGWFPCNYVERIPEGEKALSPKKALLPPTVSLSTTSAASEPLSPSKSVEESDYQNVHFSSLNVNAAWQQKSAFTRTVSPGSVSPIHGQGQPVENLKAQALCSWTAKKDNHLNFSKNDIISVLEQQENWWFGEVHGGRGWFPKSYVKLLPGSEKKEEPEAIYAAVKKKSSAQPYPAGEEYVALYSYSSSEPGDLTFTEGEEILVTQKEGEWWTGSIDDRTGIFPSNYVRPKDQEGSSNAGKTGTINKKPEIAQVTTAYAASGSEQLSLAPGQLILILKKNASGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATSAAPSVCQVIAMYDYMANNEDELSFSKGQLINVLSKDDADWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQPMERKRQGYIHELIQTEERYMDDLQLVLEVFQKPMADSGCLTEGEMGLIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGASLLQQKTDEEADFKDYLKKLALDPRCKGMPLSSFLLKPMQRITRYPLLIKSILENTPENHPDHSNLKLALERAEELCSQVNEGVREKENSDRLEWIQSHVQCEGLAEQPVFNSLTNCLGPRKLLHSGKLYKAKSSKELYGFLFNDFLLLTYMVKQFVSSGSDKLFSPKSNSQFKMYKMPVFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLKTDNINERAELLQLDIALHLLLSDVVAV

Target ORF information:

RefSeq Version	XM_015285039.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus intersectin 2 (ITSN2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285039.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC GGCCGTGAAT GGAGGGCCAA ACATATGGGC CATCACCGCG 
GAAGAGCGGG CCAAGCACGA CAAGCAGTTT GATAGCCTTA AGCCCACGGG AGGCTACATT 
ACAGGTGATC AAGCACGTAC CTTTTTCTTG CAATCAGGGC TACCATCTTC AGTCCTCGCT 
GACATATGGG CACTGTCAGA CTTGAACAAA GATGGAAAAA TGGATCAACA GGAGTTCTCT 
ATAGCCATGA AACTCATCAA GCTGAAATTA CAAGGACAGC ACTTGCCTAT GGTTCTCCCT 
CCTGTCATGA AACAAACTCC AGTACTTTCA CCTCTAATGT CAGCTCGCTT TGGAATGGGT 
AGTATGCCAA ATTTGTCCAT GCCTACATCC ATGTCTACGA TCACACCATT AGCTCCAATG 
TCTCTGACCT CAATGACATC CATTCCTCCT TTGGTTATTT CTGCTCCCCT GGTGCCTTCT 
GTCAGTACCT CATCACTGCC AAATGGAACA TCCAGTCTTC TGCAGCCCTT GGCTATACCT 
TTCTCTTCAA CGCAGCCTCA TACTGGTTCC TTAGGTCCCG TGGCAGGAGG GTTTGGTGGA 
TCTAACATGC AGAAGGCACA GTCACTAATC GATTTAGGAT CTAGCAGTTC AAATTCTTCC 
TCCAGTGCTT CGCTAGCTGG GAATTCACCA AAGATTGCAC CCTCAGACTG GGCAGTTCCT 
CAGGCTTCCA GATTAAAATA CAGACAGAAA TTTAACAGTC TTGATAAAAG TATGAGTGGA 
TATTTATCAG GATTTCAAGC AAGGAATGCC CTTCTGCAAT CAAACCTCTC ACAGACTCAG 
CTGGCTACTA TTTGGTCACT GGCTGATATT GATGGAGATG GACAGCTGAA AGCAGATGAG 
TTTGTGTTAG CCATGCACCT AACTGATATG GCCAAAGCTG GGCAACCTCT GCCACTAACT 
CTGCCTCTTG AGCTGGTACC ACCTTCCTTC AGGGGTGGAA AACTCACTGA AAATATTAAT 
GGAACTCTGC CATCTTACCA GCCTGTGCAA GAGGAGGAGC CACAGAAAAA ACAGCCTGTG 
ACGTTTGAGG ACAAAAGAAA AGCAAACTAT GAGAGAGGAA ATGTGGAGCT GGAAAAACGT 
CGTCAGGTCC TGTTGGAACA GCAACAGAGA GAGGCAGAAC GTAAGGCTCA GAAAGAAAGA 
GAAGAACAAG AACGAAGAGA GAGGGAACGT CAGGAGCAGG AACGGAAAAA ACAACTTGAA 
TTGGAAAGGC AGCTGGAGAG ACAACGAGAG CTGGAAAGAC AAAGGGAAGA AGAAAGGAGG 
AAAGAAATAG AAAGGCGGGA GGCAGCAAAA CAAGAACTTG AGCGCCAGCG GCGCTTGGAG 
TGGGAGAGAA TTCGTCGCCA AGAACTTCTT AATCAGCGTA ACAGAGAACA AGAAGAAATT 
ATCAAGTTGA CCTCTAAGAA GAAGAGCCTT AATCTTGAAT TAGAAGCTCT GACGGACAAA 
CATCAGCAAA TCTCAGGCAG ATTGCAAGAT ATTCGAAGCA AAAAGCAGAT TCGGAGGACT 
GAGCTGGAGG CTTTAGATAG AAAATGTGAC CAAGGAATTA TGGAAGTCAA GCAACTACAA 
CAGCAACTTC AGGAGTATCA GAATAAGCTA ACCTATTTAG TTCCTGAAAA GCAGTTGTTA 
ACTGAAAGAA TTAAAAACGT TCAACTCGAC AGCACTCAAA ATGCTGGAAT TGCTTCAGTG 
CAAAAGAAGT CTCTAGAAAA GGAAGAATTA TGCCAAAGAC TTAAGGAACA ATTAGATGCC 
CTTGAGAAAG AAACTGCTTC TAAACTTGCT GAAATGGATG AATTTAACAG TCAGCTTAAG 
TGTGACAGTA TGGATGATTC TGTTCTTCAG TGCCTTTTGT CTCTGCTAAG CTGTCTCAAC 
AACCTCTTCC TCTTACTTAA GGACCTGAGA GAAAGCTACA GCACGCAGCA ATTAGCCTTG 
GAGCAGCTCC ATAGGATCAA ACAGAGCAAA ATAAGAGAGG TAGAGAAACG AAGAGCTGAA 
CTTGCGCAGA AAAAGCGGCT GGAAGATGAG GCTACAAGGA AAGCAAAACG GGAGAAAGAG 
AACCGATGGC AGGAAAATAT CCGAAGGGAA GAGGAGGAGA AAAAGAAACG ATTAGAGGAA 
GAGAGAATGC AGGAAAAGGC TCAGGAAAAG CTCCATGAAG AGGAGGAGAA AAATAAGCAA 
GCCGGGATCT TGAGAGAAAC AGAACAACGA CAGAGGCAGC TGGAGGAAGA GAAAAGAAAG 
CAAGAACAAA TGGCAAAAGA AGCTGAGGAG AGGCGTAAGC AGAATGGAGA AATAAAGAAG 
ACAAAAGAAG TCACCACCTC TCATGAGACA GAGAAATGCA CTGCTAAATT AAACACAAGT 
GAAAAGTTTG GAGATCTGTT GAAACCAACA GAGGGTAAAA GTCTTAAGCC ACCCTGGACA 
AATGAAGGCA ACACCATCAG TGCTTCAGAC TCCAGAAGTT CCGCTGCCTT TGTAAATTAC 
AGAGCTTTAT ATCCATTTGA AGCAAGGAAT CATGATGAGA TGAGCTTTAA TACTGGAGAC 
ATAATTCAGG TTGATGAAAA AAATGTTGGA GAGCCTGGCT GGCTTTATGG GAGCTTCCAA 
GGACGTATTG GTTGGTTTCC ATGCAACTAT GTGGAAAGAA TACCAGAAGG AGAAAAAGCT 
TTATCTCCTA AGAAGGCCTT ACTTCCTCCC ACAGTATCTT TATCTACTAC CTCAGCTGCT 
TCAGAACCAC TTTCACCAAG CAAATCTGTA GAAGAGTCGG ATTACCAGAA CGTACATTTC 
TCCAGCCTGA ACGTTAATGC AGCGTGGCAA CAGAAGTCAG CTTTTACCCG CACTGTGTCC 
CCTGGATCTG TGTCACCCAT TCATGGCCAG GGGCAGCCTG TCGAGAACTT GAAAGCACAA 
GCACTTTGCT CTTGGACTGC AAAAAAAGAT AACCACTTGA ACTTCTCAAA AAATGATATC 
ATTTCTGTCC TGGAGCAGCA GGAAAACTGG TGGTTTGGGG AGGTGCATGG AGGAAGGGGT 
TGGTTTCCCA AGTCGTACGT CAAGCTCTTA CCTGGAAGTG AGAAGAAAGA GGAACCAGAA 
GCTATTTATG CAGCAGTAAA GAAGAAGTCC AGTGCACAGC CCTACCCAGC AGGAGAGGAG 
TATGTTGCAC TGTATTCGTA TTCAAGCTCT GAGCCTGGCG ACTTGACTTT CACCGAAGGT 
GAGGAAATCC TGGTGACCCA GAAAGAAGGG GAATGGTGGA CAGGGAGCAT TGATGACAGA 
ACTGGGATCT TCCCCTCCAA TTATGTCAGA CCAAAGGATC AAGAAGGGTC TAGTAATGCT 
GGCAAAACTG GAACAATAAA TAAGAAACCT GAAATTGCTC AGGTGACTAC TGCTTATGCT 
GCATCAGGAA GTGAACAGCT TAGTCTTGCT CCTGGGCAGC TCATACTTAT CCTGAAGAAA 
AATGCTAGTG GATGGTGGCA AGGAGAGCTG CAGGCAAGAG GGAAAAAGAG ACAGAAAGGA 
TGGTTCCCTG CCAGCCACGT GAAGTTACTG GGTCCGAGCA GTGAGCGAGC CACATCTGCT 
GCCCCCTCAG TGTGTCAAGT AATAGCAATG TATGATTACA TGGCAAACAA TGAAGACGAG 
CTCAGCTTCT CCAAAGGGCA GCTTATTAAC GTGCTGAGCA AAGATGATGC TGACTGGTGG 
CAAGGAGAAA TCAATGGTGT GACAGGTCTC TTCCCTTCCA ACTACGTGAA GATGACCACA 
GATTCTGATC CAAGTCAGCA GTGGTGTGCT GATCTCCAAA GCCTCGATAC CATGCAGCCA 
ATGGAAAGGA AGAGGCAGGG TTACATCCAC GAACTCATTC AGACGGAAGA AAGGTATATG 
GATGATCTTC AGCTTGTCCT GGAGGTTTTC CAAAAGCCAA TGGCTGATTC GGGTTGTCTC 
ACAGAAGGGG AAATGGGGCT GATCTTTGTT AACTGGAAGG AACTCATCAT GTCAAATACC 
AAGTTACTGA AAGCCTTGCG AGTGCGTAAA AAAACAGGAG GAGAAAAAAT GCCGGTGCAG 
ATGATTGGGG ACATCTTGGC AGCAGAGCTG TCACACATGC AGGCCTACAT TCGGTTCTGC 
AGCTGCCAGC TTAACGGGGC TTCATTGCTG CAGCAGAAAA CTGATGAAGA AGCTGATTTC 
AAAGACTACT TAAAGAAACT CGCTCTGGAT CCTCGCTGCA AAGGAATGCC CCTCTCCAGT 
TTCCTCCTGA AACCCATGCA AAGAATAACA CGCTATCCTC TGCTTATAAA GAGTATTCTA 
GAGAATACTC CAGAAAATCA CCCAGATCAC AGTAACCTCA AGCTTGCCTT GGAGCGTGCA 
GAAGAGCTGT GTTCTCAGGT TAATGAAGGG GTGCGTGAGA AAGAGAACTC GGACCGGCTG 
GAATGGATAC AGTCCCACGT GCAGTGTGAA GGCCTTGCTG AGCAACCTGT ATTCAATTCC 
CTCACAAACT GCTTGGGACC GCGGAAGCTC CTGCACAGCG GCAAGCTCTA CAAGGCAAAG 
AGCAGCAAGG AGCTGTATGG CTTCCTGTTC AACGACTTCC TGCTGCTTAC CTACATGGTC 
AAACAGTTTG TCTCTTCCGG CTCTGATAAA CTGTTCAGCC CCAAGTCCAA CTCCCAGTTC 
AAAATGTACA AAATGCCTGT TTTCCTTAAT GAAGTCCTAG TGAAATTGCC TACAGACCCT 
TCAAGTGATG AGCCAGTTTT CCACATATCA CACATCGACA GAGTTTACAC GCTTAAAACT 
GACAACATCA ACGAGCGAGC AGAACTCTTG CAGTTGGATA TAGCATTGCA TCTACTGCTC 
TCAGATGTCG TAGCTGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ITSN2
Entrez Gene ID	421979
Full Name	intersectin 2
General protein information	Preferred Names intersectin 2 Names intersectin-2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Macaca mulatta (Rhesus monkey)	LOC704858	XP_002808077.1
Rattus norvegicus (Norway rat)	Itsn2	XP_003750174.1
Mus musculus (house mouse)	Itsn2	NP_001185897.1
Gallus gallus (chicken)	ITSN2	XP_419989.4
Pan troglodytes (chimpanzee)	ITSN2	XP_001145237.3
Canis lupus familiaris (dog)	ITSN2	XP_849291.2
Danio rerio (zebrafish)	itsn2a	NP_001138272.1
Xenopus tropicalis (tropical clawed frog)	itsn2	XP_004918242.1
Homo sapiens (human)	ITSN2	NP_062541.3
Bos taurus (cattle)	ITSN2	XP_002691512.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ITSN2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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