alk cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	alk
Entrez Gene ID	105357017
Full Name	ALK receptor tyrosine kinase
Synonyms	alk
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011490817.2	XP_011489119.2	ALK tyrosine kinase receptor
XM_023951462.1	XP_023807230.1	ALK tyrosine kinase receptor isoform X2
XM_023951461.1	XP_023807229.1	ALK tyrosine kinase receptor isoform X1

Pathways from BioSystems
Gene Ontology

The following alk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa75963	XM_011490817.2 Latest version!	Oryzias latipes ALK receptor tyrosine kinase (LOC105357017), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OJa113643	XM_023951461.1 Latest version!	Oryzias latipes ALK receptor tyrosine kinase (alk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa113644	XM_023951462.1 Latest version!	Oryzias latipes ALK receptor tyrosine kinase (alk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alk ( XM_011490817.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011489119.2 Oryzias latipes ALK receptor tyrosine kinase (LOC105357017), mRNA.

Price & Availability↑

CloneID	OJa75963
Clone ID Related Accession (Same CDS sequence)	XM_011490817.2
Accession Version	XM_011490817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	ALK tyrosine kinase receptor
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011490817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAACGCCG TGGTTTTGTT TTGGTTTTGC GCGGCGTTCG TGGCTGGGCT TCACTGCGCG 
TCGCCAAGGA CCACGGCGGG GGCTGAGCTC CCCGCGGCCG GCTTCAGCAA CAACATCAGC 
GGCGGCGGAG GTAACAGCGG GGAAACCGGA GGCAAAAGAG ACATGAACCA GACCATCATT 
TCCAGGAAAA AGAGAAAGGA TCTGTCGGTG GATTTTGCAG TCCCCTCTCT GCTTCGCTAT 
TATCTGGCGG AGTTTGTAAA GAGACCCCTG AATGGGGACT GTCAGACTTT TATCGGATGT 
TACACAGTGC GAGCAAACTT GGAAATGCAT TGCACACCTT TGCAGAAAAT CATCGCTACT 
TTTGTGGAGC CGAAATCTAC TGATTCAGCA CCAGGGAACT CGTCCTCTGA TGGACAGCTC 
CCTTTTGTGT ACAAGCGGGC GCTGTCCAAA GTTCTGAAAT TGGGCTTAAC GAAAGCAAGC 
AGGAAATCGA ACCAGGTTGT GGTGGAAATA GGAGAGGAGT TGATCAAAAC GGGATGTGGG 
GGGCTGACTG TGTTCGAGGA GGCCCCAGAG CTCATGCTGG AGATGGACCT CACGACCATC 
CTTGAGTGGT GGCTAGGAGC CGAGGGGGGG CGGCTCAGGG TTCGGCTCAT GCCCGAGAAA 
AAGGCGCAGG TGCCCGGAAT GGAGGTCCGG TACACAGAGG CGATCCGGGC TGCAGACCCC 
CGTCTATACC TGCAAATATC CTCGCAGGGT GAACCATCTG ATAGCAGAGC TGCTGCTGGC 
TTTCCTCGAG GTTATTGGAA CTTTTCCTGG GTGGCAGAAG ACAACCTAAG CTTCCGAGAT 
GGATCCCTGC CCAAATCAGT GGATCATCGA TGTCATGGCC ACAGCAGAAC TTGTGACCAC 
CAACCAGATG ACCCCCATGA GTTCACCTGG AGTATAGTGG CAGCGGGGGA CTTGCGGGAG 
GAAAGGAATG GAAAAAACGA GAAGATGACT GGAAGCCCTC AGGGTTGGGA GGAAGGTCAG 
TTTTTGTGGG TGAACAGTTC AGCCTTTGGA GGGCCCTGGG TGCTGAGTCC ATGGCTTTGG 
GGAGGCCAGG GTCTGTGCAG CCTCGACATG GCTGTGTTGC TTCATCCAAA GCAGAGCGGC 
CAATTCACCA TTTGGCTCAT AGAGCGAGAC AAGCCACCCG TGGCCCTTTT TTCCACTGAC 
ACACTGCCAC GCATCACAGG CTGGGCTGTG GTCCACCTCA CTCTGGGCAC ACATGGAGGA 
TCCCCTTTTC GGATTTCAGC CAGCTACGAC CAGCGGATCC CATTTGATGA TACTGCTACC 
ATTGAGCCAC ACTTTACTAC CAACTGCACC ATGGCATCCT CGCCTGGACC AAACATCACA 
CTGGAGGGTT TGTACCACTG TCACCAGGGA TTTGGAATCC CACTAGACAA ACTGTGCGAC 
TTCAGGACCG ACTGTCCTCT TGGAGATGAT GAGGGAGTCC TTTGTCGACA CTTTCTCAAT 
GGCAGCTATT GCTCTTTTGA AGAGGATGAG TGCAGCTGGC AGTCTGTGGC AGGTCGTGGT 
CCTTCCTGGA AAAGGTTCCA GTCACCCTCC AAGGCAACCA GACAGAGCTG CCCGTCATCA 
GGTGCTACAT ACACCGTGGA GGGAGGCCAA TCAAAAGGTC AACATGGCTC AGCAGTGCTA 
AAGAGTCCAC TTTTCCCTCC TCCTCTGAGA AATGCTCCAT GCACGGTGCA GTTCTGGCTG 
TGCTCTGGCG GTTTGCAGAA AGGCTCGGTG TCTTTGTGGA TCCTGGAGAA CAGCACAGGC 
CCTGATTCAT TGCGCAGACT GTGGCATTCA GCCAGCGAGC CTAAATCTGA CCGAGGCTGG 
AAGCTCATCA CAGTTGCCCT TTACGGGCTT GCAGACTGGT TCTGGCTGCA GTTCAGTGCA 
GATGAAGGGC CCGGACCAGG TTCAGCCATC GCTCTGGACA ACATCTCTTT CAGTATGAAC 
TGTTTCTTGA CATGTGAGTA CTACAACCAA AAGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011489119.2
CDS	806..2824
Translation	MNAVVLFWFCAAFVAGLHCASPRTTAGAELPAAGFSNNISGGGGNSGETGGKRDMNQTIISRKKRKDLSVDFAVPSLLRYYLAEFVKRPLNGDCQTFIGCYTVRANLEMHCTPLQKIIATFVEPKSTDSAPGNSSSDGQLPFVYKRALSKVLKLGLTKASRKSNQVVVEIGEELIKTGCGGLTVFEEAPELMLEMDLTTILEWWLGAEGGRLRVRLMPEKKAQVPGMEVRYTEAIRAADPRLYLQISSQGEPSDSRAAAGFPRGYWNFSWVAEDNLSFRDGSLPKSVDHRCHGHSRTCDHQPDDPHEFTWSIVAAGDLREERNGKNEKMTGSPQGWEEGQFLWVNSSAFGGPWVLSPWLWGGQGLCSLDMAVLLHPKQSGQFTIWLIERDKPPVALFSTDTLPRITGWAVVHLTLGTHGGSPFRISASYDQRIPFDDTATIEPHFTTNCTMASSPGPNITLEGLYHCHQGFGIPLDKLCDFRTDCPLGDDEGVLCRHFLNGSYCSFEEDECSWQSVAGRGPSWKRFQSPSKATRQSCPSSGATYTVEGGQSKGQHGSAVLKSPLFPPPLRNAPCTVQFWLCSGGLQKGSVSLWILENSTGPDSLRRLWHSASEPKSDRGWKLITVALYGLADWFWLQFSADEGPGPGSAIALDNISFSMNCFLTCEYYNQKY

Target ORF information:

RefSeq Version	XM_011490817.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ALK receptor tyrosine kinase (LOC105357017), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490817.2

ORF Insert Sequence:

ATGAACGCCG TGGTTTTGTT TTGGTTTTGC GCGGCGTTCG TGGCTGGGCT TCACTGCGCG 
TCGCCAAGGA CCACGGCGGG GGCTGAGCTC CCCGCGGCCG GCTTCAGCAA CAACATCAGC 
GGCGGCGGAG GTAACAGCGG GGAAACCGGA GGCAAAAGAG ACATGAACCA GACCATCATT 
TCCAGGAAAA AGAGAAAGGA TCTGTCGGTG GATTTTGCAG TCCCCTCTCT GCTTCGCTAT 
TATCTGGCGG AGTTTGTAAA GAGACCCCTG AATGGGGACT GTCAGACTTT TATCGGATGT 
TACACAGTGC GAGCAAACTT GGAAATGCAT TGCACACCTT TGCAGAAAAT CATCGCTACT 
TTTGTGGAGC CGAAATCTAC TGATTCAGCA CCAGGGAACT CGTCCTCTGA TGGACAGCTC 
CCTTTTGTGT ACAAGCGGGC GCTGTCCAAA GTTCTGAAAT TGGGCTTAAC GAAAGCAAGC 
AGGAAATCGA ACCAGGTTGT GGTGGAAATA GGAGAGGAGT TGATCAAAAC GGGATGTGGG 
GGGCTGACTG TGTTCGAGGA GGCCCCAGAG CTCATGCTGG AGATGGACCT CACGACCATC 
CTTGAGTGGT GGCTAGGAGC CGAGGGGGGG CGGCTCAGGG TTCGGCTCAT GCCCGAGAAA 
AAGGCGCAGG TGCCCGGAAT GGAGGTCCGG TACACAGAGG CGATCCGGGC TGCAGACCCC 
CGTCTATACC TGCAAATATC CTCGCAGGGT GAACCATCTG ATAGCAGAGC TGCTGCTGGC 
TTTCCTCGAG GTTATTGGAA CTTTTCCTGG GTGGCAGAAG ACAACCTAAG CTTCCGAGAT 
GGATCCCTGC CCAAATCAGT GGATCATCGA TGTCATGGCC ACAGCAGAAC TTGTGACCAC 
CAACCAGATG ACCCCCATGA GTTCACCTGG AGTATAGTGG CAGCGGGGGA CTTGCGGGAG 
GAAAGGAATG GAAAAAACGA GAAGATGACT GGAAGCCCTC AGGGTTGGGA GGAAGGTCAG 
TTTTTGTGGG TGAACAGTTC AGCCTTTGGA GGGCCCTGGG TGCTGAGTCC ATGGCTTTGG 
GGAGGCCAGG GTCTGTGCAG CCTCGACATG GCTGTGTTGC TTCATCCAAA GCAGAGCGGC 
CAATTCACCA TTTGGCTCAT AGAGCGAGAC AAGCCACCCG TGGCCCTTTT TTCCACTGAC 
ACACTGCCAC GCATCACAGG CTGGGCTGTG GTCCACCTCA CTCTGGGCAC ACATGGAGGA 
TCCCCTTTTC GGATTTCAGC CAGCTACGAC CAGCGGATCC CATTTGATGA TACTGCTACC 
ATTGAGCCAC ACTTTACTAC CAACTGCACC ATGGCATCCT CGCCTGGACC AAACATCACA 
CTGGAGGGTT TGTACCACTG TCACCAGGGA TTTGGAATCC CACTAGACAA ACTGTGCGAC 
TTCAGGACCG ACTGTCCTCT TGGAGATGAT GAGGGAGTCC TTTGTCGACA CTTTCTCAAT 
GGCAGCTATT GCTCTTTTGA AGAGGATGAG TGCAGCTGGC AGTCTGTGGC AGGTCGTGGT 
CCTTCCTGGA AAAGGTTCCA GTCACCCTCC AAGGCAACCA GACAGAGCTG CCCGTCATCA 
GGTGCTACAT ACACCGTGGA GGGAGGCCAA TCAAAAGGTC AACATGGCTC AGCAGTGCTA 
AAGAGTCCAC TTTTCCCTCC TCCTCTGAGA AATGCTCCAT GCACGGTGCA GTTCTGGCTG 
TGCTCTGGCG GTTTGCAGAA AGGCTCGGTG TCTTTGTGGA TCCTGGAGAA CAGCACAGGC 
CCTGATTCAT TGCGCAGACT GTGGCATTCA GCCAGCGAGC CTAAATCTGA CCGAGGCTGG 
AAGCTCATCA CAGTTGCCCT TTACGGGCTT GCAGACTGGT TCTGGCTGCA GTTCAGTGCA 
GATGAAGGGC CCGGACCAGG TTCAGCCATC GCTCTGGACA ACATCTCTTT CAGTATGAAC 
TGTTTCTTGA CATGTGAGTA CTACAACCAA AAGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alk ( XM_023951461.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023807229.1 Oryzias latipes ALK receptor tyrosine kinase (alk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa113643
Clone ID Related Accession (Same CDS sequence)	XM_023951461.1
Accession Version	XM_023951461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	ALK tyrosine kinase receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCCG TGGTTTTGTT TTGGTTTTGC GCGGCGTTCG TGGCTGGGCT TCACTGCGCG 
TCGCCAAGGA CCACGGCGGG GGCTGAGCTC CCCGCGGCCG GCTTCAGCAA CAACATCAGC 
GGCGGCGGAG GTAACAGCGG GGAAACCGGA GGCAAAAGAG ACATGAACCA GACCATCATT 
TCCAGGAAAA AGAGAAAGGA TCTGTCGGTG GATTTTGCAG TCCCCTCTCT GCTTCGCTAT 
TATCTGGCGG AGTTTGTAAA GAGACCCCTG AATGGGGACT GTCAGACTTT TATCGGATGT 
TACACAGTGC GAGCAAACTT GGAAATGCAT TGCACACCTT TGCAGAAAAT CATCGCTACT 
TTTGTGGAGC CGAAATCTAC TGATTCAGCA CCAGGGAACT CGTCCTCTGA TGGACAGCTC 
CCTTTTGTGT ACAAGCGGGC GCTGTCCAAA GTTCTGAAAT TGGGCTTAAC GAAAGCAAGC 
AGGAAATCGA ACCAGGTTGT GGTGGAAATA GGAGAGGAGT TGATCAAAAC GGGATGTGGG 
GGGCTGACTG TGTTCGAGGA GGCCCCAGAG CTCATGCTGG AGATGGACCT CACGACCATC 
CTTGAGTGGT GGCTAGGAGC CGAGGGGGGG CGGCTCAGGG TTCGGCTCAT GCCCGAGAAA 
AAGGCGCAGG TGCCCGGAAT GGAGGTCCGG TACACAGAGG CGATCCGGGC TGCAGACCCC 
CGTCTATACC TGCAAATATC CTCGCAGGGT GAACCATCTG ATAGCAGAGC TGCTGCTGGC 
TTTCCTCGAG GTTATTGGAA CTTTTCCTGG GTGGCAGAAG ACAACCTAAG CTTCCGAGAT 
GGATCCCTGC CCAAATCAGT GGATCATCGA TGTCATGGCC ACAGCAGAAC TTGTGACCAC 
CAACCAGATG ACCCCCATGA GTTCACCTGG AGTATAGTGG CAGCGGGGGA CTTGCGGGAG 
GAAAGGAATG GAAAAAACGA GAAGATGACT GGAAGCCCTC AGGGTTGGGA GGAAGGTAAG 
AATGAAGGTC AGTTTTTGTG GGTGAACAGT TCAGCCTTTG GAGGGCCCTG GGTGCTGAGT 
CCATGGCTTT GGGGAGGCCA GGGTCTGTGC AGCCTCGACA TGGCTGTGTT GCTTCATCCA 
AAGCAGAGCG GCCAATTCAC CATTTGGCTC ATAGAGCGAG ACAAGCCACC CGTGGCCCTT 
TTTTCCACTG ACACACTGCC ACGCATCACA GGCTGGGCTG TGGTCCACCT CACTCTGGGC 
ACACATGGAG GATCCCCTTT TCGGATTTCA GCCAGCTACG ACCAGCGGAT CCCATTTGAT 
GATACTGCTA CCATTGAGCC ACACTTTACT ACCAACTGCA CCATGGCATC CTCGCCTGGA 
CCAAACATCA CACTGGAGGG TTTGTACCAC TGTCACCAGG GATTTGGAAT CCCACTAGAC 
AAACTGTGCG ACTTCAGGAC CGACTGTCCT CTTGGAGATG ATGAGGGAGT CCTTTGTCGA 
CACTTTCTCA ATGGCAGCTA TTGCTCTTTT GAAGAGGATG AGTGCAGCTG GCAGTCTGTG 
GCAGGTCGTG GTCCTTCCTG GAAAAGGTTC CAGTCACCCT CCAAGGCAAC CAGACAGAGC 
TGCCCGTCAT CAGGTGCTAC ATACACCGTG GAGGGAGGCC AATCAAAAGG TCAACATGGC 
TCAGCAGTGC TAAAGAGTCC ACTTTTCCCT CCTCCTCTGA GAAATGCTCC ATGCACGGTG 
CAGTTCTGGC TGTGCTCTGG CGGTTTGCAG AAAGGCTCGG TGTCTTTGTG GATCCTGGAG 
AACAGCACAG GCCCTGATTC ATTGCGCAGA CTGTGGCATT CAGCCAGCGA GCCTAAATCT 
GACCGAGGCT GGAAGCTCAT CACAGTTGCC CTTTACGGGC TTGCAGACTG GTTCTGGCTG 
CAGTTCAGTG CAGATGAAGG GCCCGGACCA GGTTCAGCCA TCGCTCTGGA CAACATCTCT 
TTCAGTATGA ACTGTTTCTT GACATCCAAT GGCGAGTTTC CACCAGTGGC CCCCAGCTCA 
ACCAGGCTAC CCTTTAGTCC GGCCATCAAG ACAACGCCCC CATCGATTAG AAGCCCTCCA 
GTGAACTTCC CCACCCCAGA ATACTCGAAA AAATGGATTT TCAACACCTG TGGGGCCGTG 
GGCCATGAGG GCCCGACTCC CACCCAGTGT CTTAACTCCT ACAGGAGCAG CAATGTAAAC 
GTGACAGTCG GCACCCGAGG GCCCTACAAA GGCATTCAGA TGTGGGAGGT CCCAGAAACG 
GGCACCTACA GGATCACCGC GTACGGTGCA GCTGGCGGGC GGAGCGTGGT GGCCATGAGC 
AGGTCACATG GTGTCTACAT CACAGGAGAC TTCCTGCTGA GGAAAGGCGA GCTGCTCCTC 
ATCCTGGTGG GACAGAAAGG AGAAGATGCC TGCCCCAATG CCAACACCAT ACTAAACAAG 
ATCTGCCAGG AGCAGAGCAG CTCCATGGTG GACAAGAGTC AAGTGAAGGG AGGGGGAGGA 
GGAGGTGGTG GGGCTACATA CGTGTTCAAG GTGGACAGAG AAGTGTACAT CCCCCTCATT 
ATCGCTGCTG GCGGAGGAGG CAGAGGCTAC AACAGCCAGT CAGAGACCCA GCTGGAGCAG 
ATGGATTATG ACCCCAACCA ACTGGGGCGC AATGGGAAGT CCCACTCTGC AGGTGGTGGC 
GGAGGCTGGA ATGACAGTGC TCCTGTCAGC CAAGGAGGCA GACCACTGGT GCTGGGGGGC 
CAGGGAGGGC AGGCCTGTCA AAAGTTCTGG CAGACCCGAG GTGGCTTTGG AGGCGGTGGG 
GGTGGATGTC TGTCGGGCGG AGGTGGCGGA GGCTACAGAG GTGGAAACAC CTCCATAGAG 
GACAACCCCA CAGCTGATGG TGATGATGGC ACATCTTTTC TAGGCCCAGA TGGAGAACCC 
TTCCTCTTCC CGCTCAAAGC CATCGATGGT GACGGTGAAG TGATCATCAG CCCCGTTCAG 
AATTGCAGCC ACTGTGAGAG TAGAGAATGC CACGAGAGCA ACGATGGCAT CATCTGCTAC 
TGCGACGAAG ACCTCATCCT GGCTGCAGAC AGAGTGTCCT GCATCAACGC CTCAGGAGTG 
AACCCGCTGT CTCAACCCCC GTCCCTCTCC CACCTGGCTC TGGGTCTGTC TGTGGGCACC 
TCGGCCCTCA TCGCGGCCTT GCTGCTGGCT GTGTCCGGAG TCATGATAAT GTACAGGCGT 
AAACACACAG AGCTGCAGTC TATCCAGCTG GAGCTGCAGA GTCCAGACTG CAAACTCAGC 
AAGCTGCGAG CCTCCACCAT CATGACTGAC TACAACCCCA ACTACTGCTT TGCTGGCAAG 
ACAGCGTCCG TCAATGACCT GAAGGAAGTG CCGCGGAGAA ACATTTCCCT CACCAGGGGT 
CTGGGTCACG GGGCTTTCGG TGAAGTCTAT GAGGGACTGG CAGTTGGGAT ACCAGGAGAA 
CAAAGTCCAC TGCAGGTGGC AGTAAAGACC CTTCCTGAAG TTTGCTCTGA GCAAGACGAG 
CTGGACTTCC TCATGGAGGC TCTAATTATC AGCAAGTTCA GCCACCAGAA CATTGTACAT 
TGCATCGGAG TGTGTCTACA GGCAATGCCT AGGTTCATCC TGCTGGAGCT GATGGCTGGT 
GGAGACCTAA AGACTTTCCT GAGGGAGACC AGACCCCGAC TGGAGCAACC CTCCAGCCTG 
ACGATGGTGG ACCTTCTCAA TGTAGCCAGA GACATCGCTA AGGGCTGCCA GTATCTAGAA 
GAAAACCAGT TTATCCACAG GGACATCGCC GCTCGGAACT GCCTCCTCAC CTGCAAAGGA 
CCAGGCCGGG TGGCCAAAAT TGGGGATTTC GGGATGGCTC GGGACATTTA CAGGGCCAGC 
TATTATAGGA AGGGTGGGCG CGCCATGCTG CCGGTGAAAT GGATGCCACC TGAAGCCTTC 
ATGGAGGGCA TCTTCACCTC CAAAACAGAC ACATGGTCAT TTGGGGTGTT GCTCTGGGAG 
ATCTTCTCGC TGGGTTATAT GCCATACCCC AGTCGCAGTA ACCAGGAAGT GCTGGAGTTT 
GTAACCAATG GAGGAAGGAT GGACCCCCCG AAAAACTGCC CGGGGCCAGT GTATCGTATA 
ATGACTCAGA GCTGGCAGCA CCAGCCTGAA GACAGACCCA ACTTCTCAAC CATCCTGGAG 
AGGCTCGACT ACTGTCTTCA GGATCCCGAT GTTGTGAACA CGCCTCTGCC TGTTGAGTAC 
GGACCACTCC CCGAGGAGGA GGAGCGTGTC GCCATGTGCA CGGATGACCC TGCAGTGCCG 
GCTGTGCTGG TAAATTCCCA GGTGCCTGCT GGGGTGGCTC CACTTCCTCC ACCACCATCG 
TATCCATCTG AGGAGAGGAG GGAAGGGGAG CACCACGCAG TGATGAGCGC TGCGTCGGAG 
GTCAAAGCAC AGATGTCCTC TCTGCCCCAA CCCTGCCCCC ACCAGCACCA GCAGCCCCAC 
ACACAAACCT CTGTTGCCAT GACTACCACC AACACAGTCA CCTCTGTGGC CCCCTCCACA 
CTCACTGCCA AGGGACTTCA CGTTGCTACC CAGCCAAACC CAGAAGGGGG ACACATCAAC 
CTTGCCTTCA TGCAGGGCCA ACCCCAAGAA AAGGAGGGCC CCAACGGGAA GAACACCAGT 
CTCTGGAATC CCACCTACGG CTCCTGGTTC CTGCAGCAGC AGATGAAGCA GCAGCATCAG 
CGGCAGGCGG GGGTGGGAGG AGCCGGAGGA ACTAGTGGTA GGGTGCCCGG TGAGGGGCAG 
GAGCACGTAA GCCGGACGGT GTCTGAGGTG GCGGGGGTGC TAGGTCTGCA GCACCAGCAC 
AAGCAGTTTC AGCATCAGAT GCAGCTGCTG CACCATCAGC AGCAGCAACA GGCTCTTCAC 
AGGCCACTCT TACCTCCACC CCCTCCACCT GTTGCACAGT CACCTCTGCT TTTAGACTCA 
ACTGCCCTGG CTCCAGTCCC CCTCTATAGA CTGAGACGTT TCCCGTGTGG GAACATCGGA 
TACGGCTACC AGGAGCACAC TCTGCCGATG GAGGCAACCC ACGCTAACAG CAACCCCCCC 
TCATCACAGC AAGGCACACC CATGCCCCCT GCAGCGGCCC ACCAAAGGGG GTCCTCTGGT 
CCTCCTCCAA TGTCTTTGCC TCGGTTGGGA ATGTCAGAGG ACAGCCGCCC CCTCCTGGTT 
ACCATGGGGA CCGTGCAGGA CTCCAGGCTC CCCAGGATGG AGGGCCACAA TGCCACGGTG 
CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023807229.1
CDS	785..6070
Translation	MNAVVLFWFCAAFVAGLHCASPRTTAGAELPAAGFSNNISGGGGNSGETGGKRDMNQTIISRKKRKDLSVDFAVPSLLRYYLAEFVKRPLNGDCQTFIGCYTVRANLEMHCTPLQKIIATFVEPKSTDSAPGNSSSDGQLPFVYKRALSKVLKLGLTKASRKSNQVVVEIGEELIKTGCGGLTVFEEAPELMLEMDLTTILEWWLGAEGGRLRVRLMPEKKAQVPGMEVRYTEAIRAADPRLYLQISSQGEPSDSRAAAGFPRGYWNFSWVAEDNLSFRDGSLPKSVDHRCHGHSRTCDHQPDDPHEFTWSIVAAGDLREERNGKNEKMTGSPQGWEEGKNEGQFLWVNSSAFGGPWVLSPWLWGGQGLCSLDMAVLLHPKQSGQFTIWLIERDKPPVALFSTDTLPRITGWAVVHLTLGTHGGSPFRISASYDQRIPFDDTATIEPHFTTNCTMASSPGPNITLEGLYHCHQGFGIPLDKLCDFRTDCPLGDDEGVLCRHFLNGSYCSFEEDECSWQSVAGRGPSWKRFQSPSKATRQSCPSSGATYTVEGGQSKGQHGSAVLKSPLFPPPLRNAPCTVQFWLCSGGLQKGSVSLWILENSTGPDSLRRLWHSASEPKSDRGWKLITVALYGLADWFWLQFSADEGPGPGSAIALDNISFSMNCFLTSNGEFPPVAPSSTRLPFSPAIKTTPPSIRSPPVNFPTPEYSKKWIFNTCGAVGHEGPTPTQCLNSYRSSNVNVTVGTRGPYKGIQMWEVPETGTYRITAYGAAGGRSVVAMSRSHGVYITGDFLLRKGELLLILVGQKGEDACPNANTILNKICQEQSSSMVDKSQVKGGGGGGGGATYVFKVDREVYIPLIIAAGGGGRGYNSQSETQLEQMDYDPNQLGRNGKSHSAGGGGGWNDSAPVSQGGRPLVLGGQGGQACQKFWQTRGGFGGGGGGCLSGGGGGGYRGGNTSIEDNPTADGDDGTSFLGPDGEPFLFPLKAIDGDGEVIISPVQNCSHCESRECHESNDGIICYCDEDLILAADRVSCINASGVNPLSQPPSLSHLALGLSVGTSALIAALLLAVSGVMIMYRRKHTELQSIQLELQSPDCKLSKLRASTIMTDYNPNYCFAGKTASVNDLKEVPRRNISLTRGLGHGAFGEVYEGLAVGIPGEQSPLQVAVKTLPEVCSEQDELDFLMEALIISKFSHQNIVHCIGVCLQAMPRFILLELMAGGDLKTFLRETRPRLEQPSSLTMVDLLNVARDIAKGCQYLEENQFIHRDIAARNCLLTCKGPGRVAKIGDFGMARDIYRASYYRKGGRAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSRSNQEVLEFVTNGGRMDPPKNCPGPVYRIMTQSWQHQPEDRPNFSTILERLDYCLQDPDVVNTPLPVEYGPLPEEEERVAMCTDDPAVPAVLVNSQVPAGVAPLPPPPSYPSEERREGEHHAVMSAASEVKAQMSSLPQPCPHQHQQPHTQTSVAMTTTNTVTSVAPSTLTAKGLHVATQPNPEGGHINLAFMQGQPQEKEGPNGKNTSLWNPTYGSWFLQQQMKQQHQRQAGVGGAGGTSGRVPGEGQEHVSRTVSEVAGVLGLQHQHKQFQHQMQLLHHQQQQQALHRPLLPPPPPPVAQSPLLLDSTALAPVPLYRLRRFPCGNIGYGYQEHTLPMEATHANSNPPSSQQGTPMPPAAAHQRGSSGPPPMSLPRLGMSEDSRPLLVTMGTVQDSRLPRMEGHNATVL

Target ORF information:

RefSeq Version	XM_023951461.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ALK receptor tyrosine kinase (alk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023951461.1

ORF Insert Sequence:

ATGAACGCCG TGGTTTTGTT TTGGTTTTGC GCGGCGTTCG TGGCTGGGCT TCACTGCGCG 
TCGCCAAGGA CCACGGCGGG GGCTGAGCTC CCCGCGGCCG GCTTCAGCAA CAACATCAGC 
GGCGGCGGAG GTAACAGCGG GGAAACCGGA GGCAAAAGAG ACATGAACCA GACCATCATT 
TCCAGGAAAA AGAGAAAGGA TCTGTCGGTG GATTTTGCAG TCCCCTCTCT GCTTCGCTAT 
TATCTGGCGG AGTTTGTAAA GAGACCCCTG AATGGGGACT GTCAGACTTT TATCGGATGT 
TACACAGTGC GAGCAAACTT GGAAATGCAT TGCACACCTT TGCAGAAAAT CATCGCTACT 
TTTGTGGAGC CGAAATCTAC TGATTCAGCA CCAGGGAACT CGTCCTCTGA TGGACAGCTC 
CCTTTTGTGT ACAAGCGGGC GCTGTCCAAA GTTCTGAAAT TGGGCTTAAC GAAAGCAAGC 
AGGAAATCGA ACCAGGTTGT GGTGGAAATA GGAGAGGAGT TGATCAAAAC GGGATGTGGG 
GGGCTGACTG TGTTCGAGGA GGCCCCAGAG CTCATGCTGG AGATGGACCT CACGACCATC 
CTTGAGTGGT GGCTAGGAGC CGAGGGGGGG CGGCTCAGGG TTCGGCTCAT GCCCGAGAAA 
AAGGCGCAGG TGCCCGGAAT GGAGGTCCGG TACACAGAGG CGATCCGGGC TGCAGACCCC 
CGTCTATACC TGCAAATATC CTCGCAGGGT GAACCATCTG ATAGCAGAGC TGCTGCTGGC 
TTTCCTCGAG GTTATTGGAA CTTTTCCTGG GTGGCAGAAG ACAACCTAAG CTTCCGAGAT 
GGATCCCTGC CCAAATCAGT GGATCATCGA TGTCATGGCC ACAGCAGAAC TTGTGACCAC 
CAACCAGATG ACCCCCATGA GTTCACCTGG AGTATAGTGG CAGCGGGGGA CTTGCGGGAG 
GAAAGGAATG GAAAAAACGA GAAGATGACT GGAAGCCCTC AGGGTTGGGA GGAAGGTAAG 
AATGAAGGTC AGTTTTTGTG GGTGAACAGT TCAGCCTTTG GAGGGCCCTG GGTGCTGAGT 
CCATGGCTTT GGGGAGGCCA GGGTCTGTGC AGCCTCGACA TGGCTGTGTT GCTTCATCCA 
AAGCAGAGCG GCCAATTCAC CATTTGGCTC ATAGAGCGAG ACAAGCCACC CGTGGCCCTT 
TTTTCCACTG ACACACTGCC ACGCATCACA GGCTGGGCTG TGGTCCACCT CACTCTGGGC 
ACACATGGAG GATCCCCTTT TCGGATTTCA GCCAGCTACG ACCAGCGGAT CCCATTTGAT 
GATACTGCTA CCATTGAGCC ACACTTTACT ACCAACTGCA CCATGGCATC CTCGCCTGGA 
CCAAACATCA CACTGGAGGG TTTGTACCAC TGTCACCAGG GATTTGGAAT CCCACTAGAC 
AAACTGTGCG ACTTCAGGAC CGACTGTCCT CTTGGAGATG ATGAGGGAGT CCTTTGTCGA 
CACTTTCTCA ATGGCAGCTA TTGCTCTTTT GAAGAGGATG AGTGCAGCTG GCAGTCTGTG 
GCAGGTCGTG GTCCTTCCTG GAAAAGGTTC CAGTCACCCT CCAAGGCAAC CAGACAGAGC 
TGCCCGTCAT CAGGTGCTAC ATACACCGTG GAGGGAGGCC AATCAAAAGG TCAACATGGC 
TCAGCAGTGC TAAAGAGTCC ACTTTTCCCT CCTCCTCTGA GAAATGCTCC ATGCACGGTG 
CAGTTCTGGC TGTGCTCTGG CGGTTTGCAG AAAGGCTCGG TGTCTTTGTG GATCCTGGAG 
AACAGCACAG GCCCTGATTC ATTGCGCAGA CTGTGGCATT CAGCCAGCGA GCCTAAATCT 
GACCGAGGCT GGAAGCTCAT CACAGTTGCC CTTTACGGGC TTGCAGACTG GTTCTGGCTG 
CAGTTCAGTG CAGATGAAGG GCCCGGACCA GGTTCAGCCA TCGCTCTGGA CAACATCTCT 
TTCAGTATGA ACTGTTTCTT GACATCCAAT GGCGAGTTTC CACCAGTGGC CCCCAGCTCA 
ACCAGGCTAC CCTTTAGTCC GGCCATCAAG ACAACGCCCC CATCGATTAG AAGCCCTCCA 
GTGAACTTCC CCACCCCAGA ATACTCGAAA AAATGGATTT TCAACACCTG TGGGGCCGTG 
GGCCATGAGG GCCCGACTCC CACCCAGTGT CTTAACTCCT ACAGGAGCAG CAATGTAAAC 
GTGACAGTCG GCACCCGAGG GCCCTACAAA GGCATTCAGA TGTGGGAGGT CCCAGAAACG 
GGCACCTACA GGATCACCGC GTACGGTGCA GCTGGCGGGC GGAGCGTGGT GGCCATGAGC 
AGGTCACATG GTGTCTACAT CACAGGAGAC TTCCTGCTGA GGAAAGGCGA GCTGCTCCTC 
ATCCTGGTGG GACAGAAAGG AGAAGATGCC TGCCCCAATG CCAACACCAT ACTAAACAAG 
ATCTGCCAGG AGCAGAGCAG CTCCATGGTG GACAAGAGTC AAGTGAAGGG AGGGGGAGGA 
GGAGGTGGTG GGGCTACATA CGTGTTCAAG GTGGACAGAG AAGTGTACAT CCCCCTCATT 
ATCGCTGCTG GCGGAGGAGG CAGAGGCTAC AACAGCCAGT CAGAGACCCA GCTGGAGCAG 
ATGGATTATG ACCCCAACCA ACTGGGGCGC AATGGGAAGT CCCACTCTGC AGGTGGTGGC 
GGAGGCTGGA ATGACAGTGC TCCTGTCAGC CAAGGAGGCA GACCACTGGT GCTGGGGGGC 
CAGGGAGGGC AGGCCTGTCA AAAGTTCTGG CAGACCCGAG GTGGCTTTGG AGGCGGTGGG 
GGTGGATGTC TGTCGGGCGG AGGTGGCGGA GGCTACAGAG GTGGAAACAC CTCCATAGAG 
GACAACCCCA CAGCTGATGG TGATGATGGC ACATCTTTTC TAGGCCCAGA TGGAGAACCC 
TTCCTCTTCC CGCTCAAAGC CATCGATGGT GACGGTGAAG TGATCATCAG CCCCGTTCAG 
AATTGCAGCC ACTGTGAGAG TAGAGAATGC CACGAGAGCA ACGATGGCAT CATCTGCTAC 
TGCGACGAAG ACCTCATCCT GGCTGCAGAC AGAGTGTCCT GCATCAACGC CTCAGGAGTG 
AACCCGCTGT CTCAACCCCC GTCCCTCTCC CACCTGGCTC TGGGTCTGTC TGTGGGCACC 
TCGGCCCTCA TCGCGGCCTT GCTGCTGGCT GTGTCCGGAG TCATGATAAT GTACAGGCGT 
AAACACACAG AGCTGCAGTC TATCCAGCTG GAGCTGCAGA GTCCAGACTG CAAACTCAGC 
AAGCTGCGAG CCTCCACCAT CATGACTGAC TACAACCCCA ACTACTGCTT TGCTGGCAAG 
ACAGCGTCCG TCAATGACCT GAAGGAAGTG CCGCGGAGAA ACATTTCCCT CACCAGGGGT 
CTGGGTCACG GGGCTTTCGG TGAAGTCTAT GAGGGACTGG CAGTTGGGAT ACCAGGAGAA 
CAAAGTCCAC TGCAGGTGGC AGTAAAGACC CTTCCTGAAG TTTGCTCTGA GCAAGACGAG 
CTGGACTTCC TCATGGAGGC TCTAATTATC AGCAAGTTCA GCCACCAGAA CATTGTACAT 
TGCATCGGAG TGTGTCTACA GGCAATGCCT AGGTTCATCC TGCTGGAGCT GATGGCTGGT 
GGAGACCTAA AGACTTTCCT GAGGGAGACC AGACCCCGAC TGGAGCAACC CTCCAGCCTG 
ACGATGGTGG ACCTTCTCAA TGTAGCCAGA GACATCGCTA AGGGCTGCCA GTATCTAGAA 
GAAAACCAGT TTATCCACAG GGACATCGCC GCTCGGAACT GCCTCCTCAC CTGCAAAGGA 
CCAGGCCGGG TGGCCAAAAT TGGGGATTTC GGGATGGCTC GGGACATTTA CAGGGCCAGC 
TATTATAGGA AGGGTGGGCG CGCCATGCTG CCGGTGAAAT GGATGCCACC TGAAGCCTTC 
ATGGAGGGCA TCTTCACCTC CAAAACAGAC ACATGGTCAT TTGGGGTGTT GCTCTGGGAG 
ATCTTCTCGC TGGGTTATAT GCCATACCCC AGTCGCAGTA ACCAGGAAGT GCTGGAGTTT 
GTAACCAATG GAGGAAGGAT GGACCCCCCG AAAAACTGCC CGGGGCCAGT GTATCGTATA 
ATGACTCAGA GCTGGCAGCA CCAGCCTGAA GACAGACCCA ACTTCTCAAC CATCCTGGAG 
AGGCTCGACT ACTGTCTTCA GGATCCCGAT GTTGTGAACA CGCCTCTGCC TGTTGAGTAC 
GGACCACTCC CCGAGGAGGA GGAGCGTGTC GCCATGTGCA CGGATGACCC TGCAGTGCCG 
GCTGTGCTGG TAAATTCCCA GGTGCCTGCT GGGGTGGCTC CACTTCCTCC ACCACCATCG 
TATCCATCTG AGGAGAGGAG GGAAGGGGAG CACCACGCAG TGATGAGCGC TGCGTCGGAG 
GTCAAAGCAC AGATGTCCTC TCTGCCCCAA CCCTGCCCCC ACCAGCACCA GCAGCCCCAC 
ACACAAACCT CTGTTGCCAT GACTACCACC AACACAGTCA CCTCTGTGGC CCCCTCCACA 
CTCACTGCCA AGGGACTTCA CGTTGCTACC CAGCCAAACC CAGAAGGGGG ACACATCAAC 
CTTGCCTTCA TGCAGGGCCA ACCCCAAGAA AAGGAGGGCC CCAACGGGAA GAACACCAGT 
CTCTGGAATC CCACCTACGG CTCCTGGTTC CTGCAGCAGC AGATGAAGCA GCAGCATCAG 
CGGCAGGCGG GGGTGGGAGG AGCCGGAGGA ACTAGTGGTA GGGTGCCCGG TGAGGGGCAG 
GAGCACGTAA GCCGGACGGT GTCTGAGGTG GCGGGGGTGC TAGGTCTGCA GCACCAGCAC 
AAGCAGTTTC AGCATCAGAT GCAGCTGCTG CACCATCAGC AGCAGCAACA GGCTCTTCAC 
AGGCCACTCT TACCTCCACC CCCTCCACCT GTTGCACAGT CACCTCTGCT TTTAGACTCA 
ACTGCCCTGG CTCCAGTCCC CCTCTATAGA CTGAGACGTT TCCCGTGTGG GAACATCGGA 
TACGGCTACC AGGAGCACAC TCTGCCGATG GAGGCAACCC ACGCTAACAG CAACCCCCCC 
TCATCACAGC AAGGCACACC CATGCCCCCT GCAGCGGCCC ACCAAAGGGG GTCCTCTGGT 
CCTCCTCCAA TGTCTTTGCC TCGGTTGGGA ATGTCAGAGG ACAGCCGCCC CCTCCTGGTT 
ACCATGGGGA CCGTGCAGGA CTCCAGGCTC CCCAGGATGG AGGGCCACAA TGCCACGGTG 
CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alk ( XM_023951462.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023807230.1 Oryzias latipes ALK receptor tyrosine kinase (alk), transcript variant X2, mRNA.

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CloneID	OJa113644
Clone ID Related Accession (Same CDS sequence)	XM_023951462.1
Accession Version	XM_023951462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	ALK tyrosine kinase receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCCG TGGTTTTGTT TTGGTTTTGC GCGGCGTTCG TGGCTGGGCT TCACTGCGCG 
TCGCCAAGGA CCACGGCGGG GGCTGAGCTC CCCGCGGCCG GCTTCAGCAA CAACATCAGC 
GGCGGCGGAG GTAACAGCGG GGAAACCGGA GGCAAAAGAG ACATGAACCA GACCATCATT 
TCCAGGAAAA AGAGAAAGGA TCTGTCGGTG GATTTTGCAG TCCCCTCTCT GCTTCGCTAT 
TATCTGGCGG AGTTTGTAAA GAGACCCCTG AATGGGGACT GTCAGACTTT TATCGGATGT 
TACACAGTGC GAGCAAACTT GGAAATGCAT TGCACACCTT TGCAGAAAAT CATCGCTACT 
TTTGTGGAGC CGAAATCTAC TGATTCAGCA CCAGGGAACT CGTCCTCTGA TGGACAGCTC 
CCTTTTGTGT ACAAGCGGGC GCTGTCCAAA GTTCTGAAAT TGGGCTTAAC GAAAGCAAGC 
AGGAAATCGA ACCAGGTTGT GGTGGAAATA GGAGAGGAGT TGATCAAAAC GGGATGTGGG 
GGGCTGACTG TGTTCGAGGA GGCCCCAGAG CTCATGCTGG AGATGGACCT CACGACCATC 
CTTGAGTGGT GGCTAGGAGC CGAGGGGGGG CGGCTCAGGG TTCGGCTCAT GCCCGAGAAA 
AAGGCGCAGG TGCCCGGAAT GGAGGTCCGG TACACAGAGG CGATCCGGGC TGCAGACCCC 
CGTCTATACC TGCAAATATC CTCGCAGGGT GAACCATCTG ATAGCAGAGC TGCTGCTGGC 
TTTCCTCGAG GTTATTGGAA CTTTTCCTGG GTGGCAGAAG ACAACCTAAG CTTCCGAGAT 
GGATCCCTGC CCAAATCAGT GGATCATCGA TGTCATGGCC ACAGCAGAAC TTGTGACCAC 
CAACCAGATG ACCCCCATGA GTTCACCTGG AGTATAGTGG CAGCGGGGGA CTTGCGGGAG 
GAAAGGAATG GAAAAAACGA GAAGATGACT GGAAGCCCTC AGGGTTGGGA GGAAGGTCAG 
TTTTTGTGGG TGAACAGTTC AGCCTTTGGA GGGCCCTGGG TGCTGAGTCC ATGGCTTTGG 
GGAGGCCAGG GTCTGTGCAG CCTCGACATG GCTGTGTTGC TTCATCCAAA GCAGAGCGGC 
CAATTCACCA TTTGGCTCAT AGAGCGAGAC AAGCCACCCG TGGCCCTTTT TTCCACTGAC 
ACACTGCCAC GCATCACAGG CTGGGCTGTG GTCCACCTCA CTCTGGGCAC ACATGGAGGA 
TCCCCTTTTC GGATTTCAGC CAGCTACGAC CAGCGGATCC CATTTGATGA TACTGCTACC 
ATTGAGCCAC ACTTTACTAC CAACTGCACC ATGGCATCCT CGCCTGGACC AAACATCACA 
CTGGAGGGTT TGTACCACTG TCACCAGGGA TTTGGAATCC CACTAGACAA ACTGTGCGAC 
TTCAGGACCG ACTGTCCTCT TGGAGATGAT GAGGGAGTCC TTTGTCGACA CTTTCTCAAT 
GGCAGCTATT GCTCTTTTGA AGAGGATGAG TGCAGCTGGC AGTCTGTGGC AGGTCGTGGT 
CCTTCCTGGA AAAGGTTCCA GTCACCCTCC AAGGCAACCA GACAGAGCTG CCCGTCATCA 
GGTGCTACAT ACACCGTGGA GGGAGGCCAA TCAAAAGGTC AACATGGCTC AGCAGTGCTA 
AAGAGTCCAC TTTTCCCTCC TCCTCTGAGA AATGCTCCAT GCACGGTGCA GTTCTGGCTG 
TGCTCTGGCG GTTTGCAGAA AGGCTCGGTG TCTTTGTGGA TCCTGGAGAA CAGCACAGGC 
CCTGATTCAT TGCGCAGACT GTGGCATTCA GCCAGCGAGC CTAAATCTGA CCGAGGCTGG 
AAGCTCATCA CAGTTGCCCT TTACGGGCTT GCAGACTGGT TCTGGCTGCA GTTCAGTGCA 
GATGAAGGGC CCGGACCAGG TTCAGCCATC GCTCTGGACA ACATCTCTTT CAGTATGAAC 
TGTTTCTTGA CATCCAATGG CGAGTTTCCA CCAGTGGCCC CCAGCTCAAC CAGGCTACCC 
TTTAGTCCGG CCATCAAGAC AACGCCCCCA TCGATTAGAA GCCCTCCAGT GAACTTCCCC 
ACCCCAGAAT ACTCGAAAAA ATGGATTTTC AACACCTGTG GGGCCGTGGG CCATGAGGGC 
CCGACTCCCA CCCAGTGTCT TAACTCCTAC AGGAGCAGCA ATGTAAACGT GACAGTCGGC 
ACCCGAGGGC CCTACAAAGG CATTCAGATG TGGGAGGTCC CAGAAACGGG CACCTACAGG 
ATCACCGCGT ACGGTGCAGC TGGCGGGCGG AGCGTGGTGG CCATGAGCAG GTCACATGGT 
GTCTACATCA CAGGAGACTT CCTGCTGAGG AAAGGCGAGC TGCTCCTCAT CCTGGTGGGA 
CAGAAAGGAG AAGATGCCTG CCCCAATGCC AACACCATAC TAAACAAGAT CTGCCAGGAG 
CAGAGCAGCT CCATGGTGGA CAAGAGTCAA GTGAAGGGAG GGGGAGGAGG AGGTGGTGGG 
GCTACATACG TGTTCAAGGT GGACAGAGAA GTGTACATCC CCCTCATTAT CGCTGCTGGC 
GGAGGAGGCA GAGGCTACAA CAGCCAGTCA GAGACCCAGC TGGAGCAGAT GGATTATGAC 
CCCAACCAAC TGGGGCGCAA TGGGAAGTCC CACTCTGCAG GTGGTGGCGG AGGCTGGAAT 
GACAGTGCTC CTGTCAGCCA AGGAGGCAGA CCACTGGTGC TGGGGGGCCA GGGAGGGCAG 
GCCTGTCAAA AGTTCTGGCA GACCCGAGGT GGCTTTGGAG GCGGTGGGGG TGGATGTCTG 
TCGGGCGGAG GTGGCGGAGG CTACAGAGGT GGAAACACCT CCATAGAGGA CAACCCCACA 
GCTGATGGTG ATGATGGCAC ATCTTTTCTA GGCCCAGATG GAGAACCCTT CCTCTTCCCG 
CTCAAAGCCA TCGATGGTGA CGGTGAAGTG ATCATCAGCC CCGTTCAGAA TTGCAGCCAC 
TGTGAGAGTA GAGAATGCCA CGAGAGCAAC GATGGCATCA TCTGCTACTG CGACGAAGAC 
CTCATCCTGG CTGCAGACAG AGTGTCCTGC ATCAACGCCT CAGGAGTGAA CCCGCTGTCT 
CAACCCCCGT CCCTCTCCCA CCTGGCTCTG GGTCTGTCTG TGGGCACCTC GGCCCTCATC 
GCGGCCTTGC TGCTGGCTGT GTCCGGAGTC ATGATAATGT ACAGGCGTAA ACACACAGAG 
CTGCAGTCTA TCCAGCTGGA GCTGCAGAGT CCAGACTGCA AACTCAGCAA GCTGCGAGCC 
TCCACCATCA TGACTGACTA CAACCCCAAC TACTGCTTTG CTGGCAAGAC AGCGTCCGTC 
AATGACCTGA AGGAAGTGCC GCGGAGAAAC ATTTCCCTCA CCAGGGGTCT GGGTCACGGG 
GCTTTCGGTG AAGTCTATGA GGGACTGGCA GTTGGGATAC CAGGAGAACA AAGTCCACTG 
CAGGTGGCAG TAAAGACCCT TCCTGAAGTT TGCTCTGAGC AAGACGAGCT GGACTTCCTC 
ATGGAGGCTC TAATTATCAG CAAGTTCAGC CACCAGAACA TTGTACATTG CATCGGAGTG 
TGTCTACAGG CAATGCCTAG GTTCATCCTG CTGGAGCTGA TGGCTGGTGG AGACCTAAAG 
ACTTTCCTGA GGGAGACCAG ACCCCGACTG GAGCAACCCT CCAGCCTGAC GATGGTGGAC 
CTTCTCAATG TAGCCAGAGA CATCGCTAAG GGCTGCCAGT ATCTAGAAGA AAACCAGTTT 
ATCCACAGGG ACATCGCCGC TCGGAACTGC CTCCTCACCT GCAAAGGACC AGGCCGGGTG 
GCCAAAATTG GGGATTTCGG GATGGCTCGG GACATTTACA GGGCCAGCTA TTATAGGAAG 
GGTGGGCGCG CCATGCTGCC GGTGAAATGG ATGCCACCTG AAGCCTTCAT GGAGGGCATC 
TTCACCTCCA AAACAGACAC ATGGTCATTT GGGGTGTTGC TCTGGGAGAT CTTCTCGCTG 
GGTTATATGC CATACCCCAG TCGCAGTAAC CAGGAAGTGC TGGAGTTTGT AACCAATGGA 
GGAAGGATGG ACCCCCCGAA AAACTGCCCG GGGCCAGTGT ATCGTATAAT GACTCAGAGC 
TGGCAGCACC AGCCTGAAGA CAGACCCAAC TTCTCAACCA TCCTGGAGAG GCTCGACTAC 
TGTCTTCAGG ATCCCGATGT TGTGAACACG CCTCTGCCTG TTGAGTACGG ACCACTCCCC 
GAGGAGGAGG AGCGTGTCGC CATGTGCACG GATGACCCTG CAGTGCCGGC TGTGCTGGTA 
AATTCCCAGG TGCCTGCTGG GGTGGCTCCA CTTCCTCCAC CACCATCGTA TCCATCTGAG 
GAGAGGAGGG AAGGGGAGCA CCACGCAGTG ATGAGCGCTG CGTCGGAGGT CAAAGCACAG 
ATGTCCTCTC TGCCCCAACC CTGCCCCCAC CAGCACCAGC AGCCCCACAC ACAAACCTCT 
GTTGCCATGA CTACCACCAA CACAGTCACC TCTGTGGCCC CCTCCACACT CACTGCCAAG 
GGACTTCACG TTGCTACCCA GCCAAACCCA GAAGGGGGAC ACATCAACCT TGCCTTCATG 
CAGGGCCAAC CCCAAGAAAA GGAGGGCCCC AACGGGAAGA ACACCAGTCT CTGGAATCCC 
ACCTACGGCT CCTGGTTCCT GCAGCAGCAG ATGAAGCAGC AGCATCAGCG GCAGGCGGGG 
GTGGGAGGAG CCGGAGGAAC TAGTGGTAGG GTGCCCGGTG AGGGGCAGGA GCACGTAAGC 
CGGACGGTGT CTGAGGTGGC GGGGGTGCTA GGTCTGCAGC ACCAGCACAA GCAGTTTCAG 
CATCAGATGC AGCTGCTGCA CCATCAGCAG CAGCAACAGG CTCTTCACAG GCCACTCTTA 
CCTCCACCCC CTCCACCTGT TGCACAGTCA CCTCTGCTTT TAGACTCAAC TGCCCTGGCT 
CCAGTCCCCC TCTATAGACT GAGACGTTTC CCGTGTGGGA ACATCGGATA CGGCTACCAG 
GAGCACACTC TGCCGATGGA GGCAACCCAC GCTAACAGCA ACCCCCCCTC ATCACAGCAA 
GGCACACCCA TGCCCCCTGC AGCGGCCCAC CAAAGGGGGT CCTCTGGTCC TCCTCCAATG 
TCTTTGCCTC GGTTGGGAAT GTCAGAGGAC AGCCGCCCCC TCCTGGTTAC CATGGGGACC 
GTGCAGGACT CCAGGCTCCC CAGGATGGAG GGCCACAATG CCACGGTGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023807230.1
CDS	785..6058
Translation	MNAVVLFWFCAAFVAGLHCASPRTTAGAELPAAGFSNNISGGGGNSGETGGKRDMNQTIISRKKRKDLSVDFAVPSLLRYYLAEFVKRPLNGDCQTFIGCYTVRANLEMHCTPLQKIIATFVEPKSTDSAPGNSSSDGQLPFVYKRALSKVLKLGLTKASRKSNQVVVEIGEELIKTGCGGLTVFEEAPELMLEMDLTTILEWWLGAEGGRLRVRLMPEKKAQVPGMEVRYTEAIRAADPRLYLQISSQGEPSDSRAAAGFPRGYWNFSWVAEDNLSFRDGSLPKSVDHRCHGHSRTCDHQPDDPHEFTWSIVAAGDLREERNGKNEKMTGSPQGWEEGQFLWVNSSAFGGPWVLSPWLWGGQGLCSLDMAVLLHPKQSGQFTIWLIERDKPPVALFSTDTLPRITGWAVVHLTLGTHGGSPFRISASYDQRIPFDDTATIEPHFTTNCTMASSPGPNITLEGLYHCHQGFGIPLDKLCDFRTDCPLGDDEGVLCRHFLNGSYCSFEEDECSWQSVAGRGPSWKRFQSPSKATRQSCPSSGATYTVEGGQSKGQHGSAVLKSPLFPPPLRNAPCTVQFWLCSGGLQKGSVSLWILENSTGPDSLRRLWHSASEPKSDRGWKLITVALYGLADWFWLQFSADEGPGPGSAIALDNISFSMNCFLTSNGEFPPVAPSSTRLPFSPAIKTTPPSIRSPPVNFPTPEYSKKWIFNTCGAVGHEGPTPTQCLNSYRSSNVNVTVGTRGPYKGIQMWEVPETGTYRITAYGAAGGRSVVAMSRSHGVYITGDFLLRKGELLLILVGQKGEDACPNANTILNKICQEQSSSMVDKSQVKGGGGGGGGATYVFKVDREVYIPLIIAAGGGGRGYNSQSETQLEQMDYDPNQLGRNGKSHSAGGGGGWNDSAPVSQGGRPLVLGGQGGQACQKFWQTRGGFGGGGGGCLSGGGGGGYRGGNTSIEDNPTADGDDGTSFLGPDGEPFLFPLKAIDGDGEVIISPVQNCSHCESRECHESNDGIICYCDEDLILAADRVSCINASGVNPLSQPPSLSHLALGLSVGTSALIAALLLAVSGVMIMYRRKHTELQSIQLELQSPDCKLSKLRASTIMTDYNPNYCFAGKTASVNDLKEVPRRNISLTRGLGHGAFGEVYEGLAVGIPGEQSPLQVAVKTLPEVCSEQDELDFLMEALIISKFSHQNIVHCIGVCLQAMPRFILLELMAGGDLKTFLRETRPRLEQPSSLTMVDLLNVARDIAKGCQYLEENQFIHRDIAARNCLLTCKGPGRVAKIGDFGMARDIYRASYYRKGGRAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSRSNQEVLEFVTNGGRMDPPKNCPGPVYRIMTQSWQHQPEDRPNFSTILERLDYCLQDPDVVNTPLPVEYGPLPEEEERVAMCTDDPAVPAVLVNSQVPAGVAPLPPPPSYPSEERREGEHHAVMSAASEVKAQMSSLPQPCPHQHQQPHTQTSVAMTTTNTVTSVAPSTLTAKGLHVATQPNPEGGHINLAFMQGQPQEKEGPNGKNTSLWNPTYGSWFLQQQMKQQHQRQAGVGGAGGTSGRVPGEGQEHVSRTVSEVAGVLGLQHQHKQFQHQMQLLHHQQQQQALHRPLLPPPPPPVAQSPLLLDSTALAPVPLYRLRRFPCGNIGYGYQEHTLPMEATHANSNPPSSQQGTPMPPAAAHQRGSSGPPPMSLPRLGMSEDSRPLLVTMGTVQDSRLPRMEGHNATVL

Target ORF information:

RefSeq Version	XM_023951462.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ALK receptor tyrosine kinase (alk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023951462.1

ORF Insert Sequence:

ATGAACGCCG TGGTTTTGTT TTGGTTTTGC GCGGCGTTCG TGGCTGGGCT TCACTGCGCG 
TCGCCAAGGA CCACGGCGGG GGCTGAGCTC CCCGCGGCCG GCTTCAGCAA CAACATCAGC 
GGCGGCGGAG GTAACAGCGG GGAAACCGGA GGCAAAAGAG ACATGAACCA GACCATCATT 
TCCAGGAAAA AGAGAAAGGA TCTGTCGGTG GATTTTGCAG TCCCCTCTCT GCTTCGCTAT 
TATCTGGCGG AGTTTGTAAA GAGACCCCTG AATGGGGACT GTCAGACTTT TATCGGATGT 
TACACAGTGC GAGCAAACTT GGAAATGCAT TGCACACCTT TGCAGAAAAT CATCGCTACT 
TTTGTGGAGC CGAAATCTAC TGATTCAGCA CCAGGGAACT CGTCCTCTGA TGGACAGCTC 
CCTTTTGTGT ACAAGCGGGC GCTGTCCAAA GTTCTGAAAT TGGGCTTAAC GAAAGCAAGC 
AGGAAATCGA ACCAGGTTGT GGTGGAAATA GGAGAGGAGT TGATCAAAAC GGGATGTGGG 
GGGCTGACTG TGTTCGAGGA GGCCCCAGAG CTCATGCTGG AGATGGACCT CACGACCATC 
CTTGAGTGGT GGCTAGGAGC CGAGGGGGGG CGGCTCAGGG TTCGGCTCAT GCCCGAGAAA 
AAGGCGCAGG TGCCCGGAAT GGAGGTCCGG TACACAGAGG CGATCCGGGC TGCAGACCCC 
CGTCTATACC TGCAAATATC CTCGCAGGGT GAACCATCTG ATAGCAGAGC TGCTGCTGGC 
TTTCCTCGAG GTTATTGGAA CTTTTCCTGG GTGGCAGAAG ACAACCTAAG CTTCCGAGAT 
GGATCCCTGC CCAAATCAGT GGATCATCGA TGTCATGGCC ACAGCAGAAC TTGTGACCAC 
CAACCAGATG ACCCCCATGA GTTCACCTGG AGTATAGTGG CAGCGGGGGA CTTGCGGGAG 
GAAAGGAATG GAAAAAACGA GAAGATGACT GGAAGCCCTC AGGGTTGGGA GGAAGGTCAG 
TTTTTGTGGG TGAACAGTTC AGCCTTTGGA GGGCCCTGGG TGCTGAGTCC ATGGCTTTGG 
GGAGGCCAGG GTCTGTGCAG CCTCGACATG GCTGTGTTGC TTCATCCAAA GCAGAGCGGC 
CAATTCACCA TTTGGCTCAT AGAGCGAGAC AAGCCACCCG TGGCCCTTTT TTCCACTGAC 
ACACTGCCAC GCATCACAGG CTGGGCTGTG GTCCACCTCA CTCTGGGCAC ACATGGAGGA 
TCCCCTTTTC GGATTTCAGC CAGCTACGAC CAGCGGATCC CATTTGATGA TACTGCTACC 
ATTGAGCCAC ACTTTACTAC CAACTGCACC ATGGCATCCT CGCCTGGACC AAACATCACA 
CTGGAGGGTT TGTACCACTG TCACCAGGGA TTTGGAATCC CACTAGACAA ACTGTGCGAC 
TTCAGGACCG ACTGTCCTCT TGGAGATGAT GAGGGAGTCC TTTGTCGACA CTTTCTCAAT 
GGCAGCTATT GCTCTTTTGA AGAGGATGAG TGCAGCTGGC AGTCTGTGGC AGGTCGTGGT 
CCTTCCTGGA AAAGGTTCCA GTCACCCTCC AAGGCAACCA GACAGAGCTG CCCGTCATCA 
GGTGCTACAT ACACCGTGGA GGGAGGCCAA TCAAAAGGTC AACATGGCTC AGCAGTGCTA 
AAGAGTCCAC TTTTCCCTCC TCCTCTGAGA AATGCTCCAT GCACGGTGCA GTTCTGGCTG 
TGCTCTGGCG GTTTGCAGAA AGGCTCGGTG TCTTTGTGGA TCCTGGAGAA CAGCACAGGC 
CCTGATTCAT TGCGCAGACT GTGGCATTCA GCCAGCGAGC CTAAATCTGA CCGAGGCTGG 
AAGCTCATCA CAGTTGCCCT TTACGGGCTT GCAGACTGGT TCTGGCTGCA GTTCAGTGCA 
GATGAAGGGC CCGGACCAGG TTCAGCCATC GCTCTGGACA ACATCTCTTT CAGTATGAAC 
TGTTTCTTGA CATCCAATGG CGAGTTTCCA CCAGTGGCCC CCAGCTCAAC CAGGCTACCC 
TTTAGTCCGG CCATCAAGAC AACGCCCCCA TCGATTAGAA GCCCTCCAGT GAACTTCCCC 
ACCCCAGAAT ACTCGAAAAA ATGGATTTTC AACACCTGTG GGGCCGTGGG CCATGAGGGC 
CCGACTCCCA CCCAGTGTCT TAACTCCTAC AGGAGCAGCA ATGTAAACGT GACAGTCGGC 
ACCCGAGGGC CCTACAAAGG CATTCAGATG TGGGAGGTCC CAGAAACGGG CACCTACAGG 
ATCACCGCGT ACGGTGCAGC TGGCGGGCGG AGCGTGGTGG CCATGAGCAG GTCACATGGT 
GTCTACATCA CAGGAGACTT CCTGCTGAGG AAAGGCGAGC TGCTCCTCAT CCTGGTGGGA 
CAGAAAGGAG AAGATGCCTG CCCCAATGCC AACACCATAC TAAACAAGAT CTGCCAGGAG 
CAGAGCAGCT CCATGGTGGA CAAGAGTCAA GTGAAGGGAG GGGGAGGAGG AGGTGGTGGG 
GCTACATACG TGTTCAAGGT GGACAGAGAA GTGTACATCC CCCTCATTAT CGCTGCTGGC 
GGAGGAGGCA GAGGCTACAA CAGCCAGTCA GAGACCCAGC TGGAGCAGAT GGATTATGAC 
CCCAACCAAC TGGGGCGCAA TGGGAAGTCC CACTCTGCAG GTGGTGGCGG AGGCTGGAAT 
GACAGTGCTC CTGTCAGCCA AGGAGGCAGA CCACTGGTGC TGGGGGGCCA GGGAGGGCAG 
GCCTGTCAAA AGTTCTGGCA GACCCGAGGT GGCTTTGGAG GCGGTGGGGG TGGATGTCTG 
TCGGGCGGAG GTGGCGGAGG CTACAGAGGT GGAAACACCT CCATAGAGGA CAACCCCACA 
GCTGATGGTG ATGATGGCAC ATCTTTTCTA GGCCCAGATG GAGAACCCTT CCTCTTCCCG 
CTCAAAGCCA TCGATGGTGA CGGTGAAGTG ATCATCAGCC CCGTTCAGAA TTGCAGCCAC 
TGTGAGAGTA GAGAATGCCA CGAGAGCAAC GATGGCATCA TCTGCTACTG CGACGAAGAC 
CTCATCCTGG CTGCAGACAG AGTGTCCTGC ATCAACGCCT CAGGAGTGAA CCCGCTGTCT 
CAACCCCCGT CCCTCTCCCA CCTGGCTCTG GGTCTGTCTG TGGGCACCTC GGCCCTCATC 
GCGGCCTTGC TGCTGGCTGT GTCCGGAGTC ATGATAATGT ACAGGCGTAA ACACACAGAG 
CTGCAGTCTA TCCAGCTGGA GCTGCAGAGT CCAGACTGCA AACTCAGCAA GCTGCGAGCC 
TCCACCATCA TGACTGACTA CAACCCCAAC TACTGCTTTG CTGGCAAGAC AGCGTCCGTC 
AATGACCTGA AGGAAGTGCC GCGGAGAAAC ATTTCCCTCA CCAGGGGTCT GGGTCACGGG 
GCTTTCGGTG AAGTCTATGA GGGACTGGCA GTTGGGATAC CAGGAGAACA AAGTCCACTG 
CAGGTGGCAG TAAAGACCCT TCCTGAAGTT TGCTCTGAGC AAGACGAGCT GGACTTCCTC 
ATGGAGGCTC TAATTATCAG CAAGTTCAGC CACCAGAACA TTGTACATTG CATCGGAGTG 
TGTCTACAGG CAATGCCTAG GTTCATCCTG CTGGAGCTGA TGGCTGGTGG AGACCTAAAG 
ACTTTCCTGA GGGAGACCAG ACCCCGACTG GAGCAACCCT CCAGCCTGAC GATGGTGGAC 
CTTCTCAATG TAGCCAGAGA CATCGCTAAG GGCTGCCAGT ATCTAGAAGA AAACCAGTTT 
ATCCACAGGG ACATCGCCGC TCGGAACTGC CTCCTCACCT GCAAAGGACC AGGCCGGGTG 
GCCAAAATTG GGGATTTCGG GATGGCTCGG GACATTTACA GGGCCAGCTA TTATAGGAAG 
GGTGGGCGCG CCATGCTGCC GGTGAAATGG ATGCCACCTG AAGCCTTCAT GGAGGGCATC 
TTCACCTCCA AAACAGACAC ATGGTCATTT GGGGTGTTGC TCTGGGAGAT CTTCTCGCTG 
GGTTATATGC CATACCCCAG TCGCAGTAAC CAGGAAGTGC TGGAGTTTGT AACCAATGGA 
GGAAGGATGG ACCCCCCGAA AAACTGCCCG GGGCCAGTGT ATCGTATAAT GACTCAGAGC 
TGGCAGCACC AGCCTGAAGA CAGACCCAAC TTCTCAACCA TCCTGGAGAG GCTCGACTAC 
TGTCTTCAGG ATCCCGATGT TGTGAACACG CCTCTGCCTG TTGAGTACGG ACCACTCCCC 
GAGGAGGAGG AGCGTGTCGC CATGTGCACG GATGACCCTG CAGTGCCGGC TGTGCTGGTA 
AATTCCCAGG TGCCTGCTGG GGTGGCTCCA CTTCCTCCAC CACCATCGTA TCCATCTGAG 
GAGAGGAGGG AAGGGGAGCA CCACGCAGTG ATGAGCGCTG CGTCGGAGGT CAAAGCACAG 
ATGTCCTCTC TGCCCCAACC CTGCCCCCAC CAGCACCAGC AGCCCCACAC ACAAACCTCT 
GTTGCCATGA CTACCACCAA CACAGTCACC TCTGTGGCCC CCTCCACACT CACTGCCAAG 
GGACTTCACG TTGCTACCCA GCCAAACCCA GAAGGGGGAC ACATCAACCT TGCCTTCATG 
CAGGGCCAAC CCCAAGAAAA GGAGGGCCCC AACGGGAAGA ACACCAGTCT CTGGAATCCC 
ACCTACGGCT CCTGGTTCCT GCAGCAGCAG ATGAAGCAGC AGCATCAGCG GCAGGCGGGG 
GTGGGAGGAG CCGGAGGAAC TAGTGGTAGG GTGCCCGGTG AGGGGCAGGA GCACGTAAGC 
CGGACGGTGT CTGAGGTGGC GGGGGTGCTA GGTCTGCAGC ACCAGCACAA GCAGTTTCAG 
CATCAGATGC AGCTGCTGCA CCATCAGCAG CAGCAACAGG CTCTTCACAG GCCACTCTTA 
CCTCCACCCC CTCCACCTGT TGCACAGTCA CCTCTGCTTT TAGACTCAAC TGCCCTGGCT 
CCAGTCCCCC TCTATAGACT GAGACGTTTC CCGTGTGGGA ACATCGGATA CGGCTACCAG 
GAGCACACTC TGCCGATGGA GGCAACCCAC GCTAACAGCA ACCCCCCCTC ATCACAGCAA 
GGCACACCCA TGCCCCCTGC AGCGGCCCAC CAAAGGGGGT CCTCTGGTCC TCCTCCAATG 
TCTTTGCCTC GGTTGGGAAT GTCAGAGGAC AGCCGCCCCC TCCTGGTTAC CATGGGGACC 
GTGCAGGACT CCAGGCTCCC CAGGATGGAG GGCCACAATG CCACGGTGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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alk cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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