LOC101169068 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101169068
Entrez Gene ID	101169068
Full Name	insulin receptor substrate 2-B-like
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023965868.1	XP_023821636.1	insulin receptor substrate 2-B isoform X2
XM_020711741.1	XP_020567400.1	insulin receptor substrate 2-B-like
XM_023965867.1	XP_023821635.1	insulin receptor substrate 2-B isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC101169068 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101169068 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa110820	XM_023965867.1 Latest version!	Oryzias latipes insulin receptor substrate 2-B (LOC101169068), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OJa74014	XM_020711741.1 Latest version!	Oryzias latipes insulin receptor substrate 2-B-like (LOC101169068), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OJa74014	XM_023965868.1 Latest version!	Oryzias latipes insulin receptor substrate 2-B (LOC101169068), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101169068 ( XM_023965867.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023821635.1 Oryzias latipes insulin receptor substrate 2-B (LOC101169068), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa110820
Clone ID Related Accession (Same CDS sequence)	XM_023965867.1
Accession Version	XM_023965867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	insulin receptor substrate 2-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCTAA CGAACCACCC GGAAGGGGGG GGCGCCGTGA TGATGGTGGA GGCCCCGCAG 
GGGGAGCCGG TGAGGAGAGC CGCGGTCACC AACGGAGCTG GCTCGGTGCG GGAACCCCCG 
TCCCCGGTAA TCGGCACCGG CTCCCGTTTC CACCCCCACC ACCACAGCCT GCAGCAGCCC 
TCCCGGGAGC CGCAGTTTCA TCTCTTTGGG GAGAAGAGCA CCGAGTCTCC GGGCCGGAGA 
GCCTGCCCAT CGCTCGGCCA GCTCCCCGCC GCCGAGGAGC CCCCCGCGGG CGGCGGTGCC 
CATGTGTTGG CGGCCGTGCA CGTCGCGAGC GCCTCTGACG CGGTGGAAGA CATCCGGAAA 
TGCGGATACC TGAGGAAGCA GAAACACGGA CACAAGCGCT TCTTCGTGCT CCGAGCCGCC 
GGGCCGCTCG GACCCAGCCG CCTGGAGTAC TACGACAGCG AGAAGAAGTT CCGGAGCAGC 
CTCCGCCACG CTGCGGGGGC CCCCGGAGCC GCCGCGGCCG CGCCTTCCCC TCCGAAAAGA 
GTGATCTTCC TGTCCCAGTG CTTCACGGTC AACAAGCGGG CGGACTCCAA GAACAAATAC 
CTGATCGCGC TCTACACCAA AGACGAGTAC TTCGCCATCG TGGCCGAGAG CGAGCAGGAG 
CAGGAGGACT GGTACGTGGC CGTCAGCGCT CTGATGAGTG AAGGCAAGCG TGGCCATGTG 
GACTCTGATG ACCTGGATGA TGGGTACGGT ACCGTCAGTC CCGGTACCGT GTTCAAAGAG 
GTGTGGCAGG TCAATGTGAA ACCTAAAGGA CTAGGTCAGA CCAAAAACCT GACCGGCGTG 
TACCGTCTGT GCCTCTCTGC CAAAACCGTT CACCTGGTGA AGCTGAACTC CGACAACCCC 
TGTGTCAACC TGCAGCTGAT GAACATCCGG CGCTGTGGAC ATTCAGAGAG CTTCTTCTTC 
ATCGAGGTGG GCCGCTCCTC TTCTATCGGG CCCGGGGAGA TCTGGATGCA GGTGGATGAC 
TCTGTTGTGG CCCAGAGCAT GCACGAGACC ATCCTGGAGA CCATGAAAGC CCTCAAGGCT 
TTCGCAGAGT TCCGGCCCAG GAGCAAGAGT CAGTCTTCGA GCTCCAACCC CATGCCCTTC 
ATCACCACGC GCCGCCACCT GGGCAACCTG CCTCCAAGCC AGACTGGGCT GCAGCGCCGA 
TCCAGAACGG AGTCGGTGGT CGGGACCCCT CCGTCCAGTA AGAGCTCAGG GGCGAGCGGT 
TATCGCTTCC GGACCTCCAG TGAGGGAGAG GGAACCATGA ACCGGCCGTT CCGCTCTGCC 
ACGGGGAGCC TGGTGCATCT GAACTCAACG CGGGCCCTCC ATGGGCGTCA GGACGCTACT 
GGCGGCGTCT CTGGAAGTGG GGGGGCTACA GGCAGTTCTG GCACCAGCAG CGGGCGCTAC 
ACGAAGGCCA TATCCGGGAC ATCCTCTTCC TACCACGCTC GCTCATCCTC CCTGCCTGTC 
TCCCACTTCC CCTCCACCAG CAGCCCGGTC AGCGTGTCCT CCAGCAGCGG GCATGGCTCC 
GTGTCGGACA CGCTCACCCG CCCATCCAGC GCCTCCATAT GCGGCTCGCC GTCTGACGGG 
GGCTTCAACT CTTCTGACGA GTACGGATCC AGTCCGGGGG ACTTTCGGTA CTTTAGGGTG 
AGGAGTAACA CGCCCGACTC TCTGGGCAAC ACCCCCCCAA TCAGAGAGGA GAGCTGTCTT 
AATGATTACA TGGCCATGGG CTGGAACCGA GATGTTTTTG GTGGTGCGGG GGACGCTTCC 
CGTGAGGAGA GCACCTCAAC AGCCGATGAC GACCGCTTGT CCTCCTCATC TCTGAGGAGG 
AGGACGCACT CTTTCTCCAG ATCTGCAGGG GGGGCAACTG GAGGCTGTGG TGTGGCGGTT 
TATCAGAAGA TGACCCAGAC CAACCTCTCA TTGGATGAGG GCTCGGATGT GGCGCTGCCG 
TTTGGTGCCG GGCTGCTCCG GGGAGGGCCC TCCTCCTCCA CTTCCTCGCT CCGCTCTGAT 
TACAGCTCCT GTTCAGAACA CAGCCAGCAG AGCCGGCCCT CCACCCTCCC CAGGACTGAG 
GCTGCTGCTG AGCGCCCACC CCTCTGTGCC CAGGAGGATA GCGGCTACAT GCCCATGATG 
TGTGGCGTCG CTGCATCGCC GCGGGACACC CCCCCTGACT ACATGCCTAT GCAACCCAGC 
TCCTACCCCC AACACAACTC CCATTCCCCC CAGTTTCACA GCCGTTCACC CCACCGACCC 
ACACAGCCGC AGGCCTCCAC AGACGCTCAA GGCTACATGA TGATGCTCCC TGGGGGCGGG 
GGCTGCACGT CTTCAGTGCT GCAGGCCTCA CCCAGTCCTC CCAGTAGTTC CAGTTCATCT 
GCTGCTACTG GAAGCTGCAG GGGTGGGGGG GGCGAGAATG GAGAGTACAT GGACATGTCT 
TACAGCAGCG GGCACAGGCT CTCAAGTGCC ACAGGTGGCC CCCCTGAAGG AGCCCCCAAG 
TCTTACAGCC CCTACTTCTC CCTCCCACGC TCCTATAAGA CCCCCACCAG AGAGAGGGAT 
GGAGAGTATG TACCCATGAG CCCCCCTGCC AAGCCAGTGT CCATACAGGA TAGAAGGGCG 
GTGGGGGGTT CTAGCACCTC TACCCCCTCC CACCCCCCAC CGCCTTACGG CGCCCACCAC 
ACGACCGCGG TGCCGGCGGA GCGGCACGTC CTGAGGCCCA CACGTCTGCC TCTGGGCAGG 
AGGAGTTTCC ACGGCCCGCT CCCGGTGACT GAAGCCTCCA CCTCAGTGGC CCCCCCCAGC 
TACTCTGAGG GTCCTTCCAG TCCTGGGGAG TACATCAACA TTGAGTTTGG GGATCAGTTT 
CCTCACCAAC AGCAGCCTCC TGCCTATCCT CTCTCTGCCC CAGATGAAGA GACCCCCCTT 
GGACCCTGCT GTTCGCCCCC CCAACCCCAG GACTACATGA GTGTTGAGGT GGGGGGTCTG 
GGAAAGAACC AGTCGCCCAG GCCTAGCCTT GTTGCCCCCT GGAACCCCCC CAGCTATATC 
AGACCTTTAG CTAGTAACCC TGGGGCGCAT GATGGGGGGC ACTGGAAGTC AGTGGGAGAA 
GATTACACTG ACATGACCTT TAACCTCAGT GGGGGTGGGA AAGCTCAGAG CACTACAGCT 
ATGCTGCAGC ATCTCTGCGT GATGGAGGGG CATTACAAAC ACACCTTCTC CTCACCCGTT 
TCTCCCCCCT GCCTGAACCG GCCACCCACG CAGCAGTCGG AGCCCAAAGT GGTACGAGCT 
GACCCCCAAG GCAGAAGAAG ACACAGTTCA GAGACGTTCT CTTCCAGCTC CACCCCTGCA 
GGAGAAGGGC TCAGCTCCTC CCCCCCCAAC TCCTCACAGG CTGACCCCAC AGCCCACTGT 
GAGTCTTACC TGGTGGGGGG ACAAGCTTCC AGATGGGCCA ACTCAGCATC CTTTGACTGT 
GTGTGGATGT CTGTGGAGGG GGTCAGAGAC TCGCCAGCTC CCCATGACTC ACCAGGAGCC 
ACAGAGGCCC CGCCCCCTTT CTCTGCCGCA GAGGCCGGAT CCGGCAGAAT GTGCAGGAAC 
GCGTCTGCCG GCTTCCAGAA CGGACTCAAC TACATCGCCT TGGAGCTAAG GGAGGAAGGG 
GTGAACACGG AGGCCGTGGC GTCTGGAGCG ACCGGCACCA ACGGGGACAC AGCTGGGGAG 
GGAACGGTGC CTCCGCCTGA GAACGGAGCC TATGCCAGTA TAGACTTCAC CAAAACGGAC 
GGAGTCGCAG CTGCCAAGGG GGGACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023821635.1
CDS	517..4326
Translation	MDLTNHPEGGGAVMMVEAPQGEPVRRAAVTNGAGSVREPPSPVIGTGSRFHPHHHSLQQPSREPQFHLFGEKSTESPGRRACPSLGQLPAAEEPPAGGGAHVLAAVHVASASDAVEDIRKCGYLRKQKHGHKRFFVLRAAGPLGPSRLEYYDSEKKFRSSLRHAAGAPGAAAAAPSPPKRVIFLSQCFTVNKRADSKNKYLIALYTKDEYFAIVAESEQEQEDWYVAVSALMSEGKRGHVDSDDLDDGYGTVSPGTVFKEVWQVNVKPKGLGQTKNLTGVYRLCLSAKTVHLVKLNSDNPCVNLQLMNIRRCGHSESFFFIEVGRSSSIGPGEIWMQVDDSVVAQSMHETILETMKALKAFAEFRPRSKSQSSSSNPMPFITTRRHLGNLPPSQTGLQRRSRTESVVGTPPSSKSSGASGYRFRTSSEGEGTMNRPFRSATGSLVHLNSTRALHGRQDATGGVSGSGGATGSSGTSSGRYTKAISGTSSSYHARSSSLPVSHFPSTSSPVSVSSSSGHGSVSDTLTRPSSASICGSPSDGGFNSSDEYGSSPGDFRYFRVRSNTPDSLGNTPPIREESCLNDYMAMGWNRDVFGGAGDASREESTSTADDDRLSSSSLRRRTHSFSRSAGGATGGCGVAVYQKMTQTNLSLDEGSDVALPFGAGLLRGGPSSSTSSLRSDYSSCSEHSQQSRPSTLPRTEAAAERPPLCAQEDSGYMPMMCGVAASPRDTPPDYMPMQPSSYPQHNSHSPQFHSRSPHRPTQPQASTDAQGYMMMLPGGGGCTSSVLQASPSPPSSSSSSAATGSCRGGGGENGEYMDMSYSSGHRLSSATGGPPEGAPKSYSPYFSLPRSYKTPTRERDGEYVPMSPPAKPVSIQDRRAVGGSSTSTPSHPPPPYGAHHTTAVPAERHVLRPTRLPLGRRSFHGPLPVTEASTSVAPPSYSEGPSSPGEYINIEFGDQFPHQQQPPAYPLSAPDEETPLGPCCSPPQPQDYMSVEVGGLGKNQSPRPSLVAPWNPPSYIRPLASNPGAHDGGHWKSVGEDYTDMTFNLSGGGKAQSTTAMLQHLCVMEGHYKHTFSSPVSPPCLNRPPTQQSEPKVVRADPQGRRRHSSETFSSSSTPAGEGLSSSPPNSSQADPTAHCESYLVGGQASRWANSASFDCVWMSVEGVRDSPAPHDSPGATEAPPPFSAAEAGSGRMCRNASAGFQNGLNYIALELREEGVNTEAVASGATGTNGDTAGEGTVPPPENGAYASIDFTKTDGVAAAKGGL

Target ORF information:

RefSeq Version	XM_023965867.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes insulin receptor substrate 2-B (LOC101169068), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023965867.1

ORF Insert Sequence:

ATGGATCTAA CGAACCACCC GGAAGGGGGG GGCGCCGTGA TGATGGTGGA GGCCCCGCAG 
GGGGAGCCGG TGAGGAGAGC CGCGGTCACC AACGGAGCTG GCTCGGTGCG GGAACCCCCG 
TCCCCGGTAA TCGGCACCGG CTCCCGTTTC CACCCCCACC ACCACAGCCT GCAGCAGCCC 
TCCCGGGAGC CGCAGTTTCA TCTCTTTGGG GAGAAGAGCA CCGAGTCTCC GGGCCGGAGA 
GCCTGCCCAT CGCTCGGCCA GCTCCCCGCC GCCGAGGAGC CCCCCGCGGG CGGCGGTGCC 
CATGTGTTGG CGGCCGTGCA CGTCGCGAGC GCCTCTGACG CGGTGGAAGA CATCCGGAAA 
TGCGGATACC TGAGGAAGCA GAAACACGGA CACAAGCGCT TCTTCGTGCT CCGAGCCGCC 
GGGCCGCTCG GACCCAGCCG CCTGGAGTAC TACGACAGCG AGAAGAAGTT CCGGAGCAGC 
CTCCGCCACG CTGCGGGGGC CCCCGGAGCC GCCGCGGCCG CGCCTTCCCC TCCGAAAAGA 
GTGATCTTCC TGTCCCAGTG CTTCACGGTC AACAAGCGGG CGGACTCCAA GAACAAATAC 
CTGATCGCGC TCTACACCAA AGACGAGTAC TTCGCCATCG TGGCCGAGAG CGAGCAGGAG 
CAGGAGGACT GGTACGTGGC CGTCAGCGCT CTGATGAGTG AAGGCAAGCG TGGCCATGTG 
GACTCTGATG ACCTGGATGA TGGGTACGGT ACCGTCAGTC CCGGTACCGT GTTCAAAGAG 
GTGTGGCAGG TCAATGTGAA ACCTAAAGGA CTAGGTCAGA CCAAAAACCT GACCGGCGTG 
TACCGTCTGT GCCTCTCTGC CAAAACCGTT CACCTGGTGA AGCTGAACTC CGACAACCCC 
TGTGTCAACC TGCAGCTGAT GAACATCCGG CGCTGTGGAC ATTCAGAGAG CTTCTTCTTC 
ATCGAGGTGG GCCGCTCCTC TTCTATCGGG CCCGGGGAGA TCTGGATGCA GGTGGATGAC 
TCTGTTGTGG CCCAGAGCAT GCACGAGACC ATCCTGGAGA CCATGAAAGC CCTCAAGGCT 
TTCGCAGAGT TCCGGCCCAG GAGCAAGAGT CAGTCTTCGA GCTCCAACCC CATGCCCTTC 
ATCACCACGC GCCGCCACCT GGGCAACCTG CCTCCAAGCC AGACTGGGCT GCAGCGCCGA 
TCCAGAACGG AGTCGGTGGT CGGGACCCCT CCGTCCAGTA AGAGCTCAGG GGCGAGCGGT 
TATCGCTTCC GGACCTCCAG TGAGGGAGAG GGAACCATGA ACCGGCCGTT CCGCTCTGCC 
ACGGGGAGCC TGGTGCATCT GAACTCAACG CGGGCCCTCC ATGGGCGTCA GGACGCTACT 
GGCGGCGTCT CTGGAAGTGG GGGGGCTACA GGCAGTTCTG GCACCAGCAG CGGGCGCTAC 
ACGAAGGCCA TATCCGGGAC ATCCTCTTCC TACCACGCTC GCTCATCCTC CCTGCCTGTC 
TCCCACTTCC CCTCCACCAG CAGCCCGGTC AGCGTGTCCT CCAGCAGCGG GCATGGCTCC 
GTGTCGGACA CGCTCACCCG CCCATCCAGC GCCTCCATAT GCGGCTCGCC GTCTGACGGG 
GGCTTCAACT CTTCTGACGA GTACGGATCC AGTCCGGGGG ACTTTCGGTA CTTTAGGGTG 
AGGAGTAACA CGCCCGACTC TCTGGGCAAC ACCCCCCCAA TCAGAGAGGA GAGCTGTCTT 
AATGATTACA TGGCCATGGG CTGGAACCGA GATGTTTTTG GTGGTGCGGG GGACGCTTCC 
CGTGAGGAGA GCACCTCAAC AGCCGATGAC GACCGCTTGT CCTCCTCATC TCTGAGGAGG 
AGGACGCACT CTTTCTCCAG ATCTGCAGGG GGGGCAACTG GAGGCTGTGG TGTGGCGGTT 
TATCAGAAGA TGACCCAGAC CAACCTCTCA TTGGATGAGG GCTCGGATGT GGCGCTGCCG 
TTTGGTGCCG GGCTGCTCCG GGGAGGGCCC TCCTCCTCCA CTTCCTCGCT CCGCTCTGAT 
TACAGCTCCT GTTCAGAACA CAGCCAGCAG AGCCGGCCCT CCACCCTCCC CAGGACTGAG 
GCTGCTGCTG AGCGCCCACC CCTCTGTGCC CAGGAGGATA GCGGCTACAT GCCCATGATG 
TGTGGCGTCG CTGCATCGCC GCGGGACACC CCCCCTGACT ACATGCCTAT GCAACCCAGC 
TCCTACCCCC AACACAACTC CCATTCCCCC CAGTTTCACA GCCGTTCACC CCACCGACCC 
ACACAGCCGC AGGCCTCCAC AGACGCTCAA GGCTACATGA TGATGCTCCC TGGGGGCGGG 
GGCTGCACGT CTTCAGTGCT GCAGGCCTCA CCCAGTCCTC CCAGTAGTTC CAGTTCATCT 
GCTGCTACTG GAAGCTGCAG GGGTGGGGGG GGCGAGAATG GAGAGTACAT GGACATGTCT 
TACAGCAGCG GGCACAGGCT CTCAAGTGCC ACAGGTGGCC CCCCTGAAGG AGCCCCCAAG 
TCTTACAGCC CCTACTTCTC CCTCCCACGC TCCTATAAGA CCCCCACCAG AGAGAGGGAT 
GGAGAGTATG TACCCATGAG CCCCCCTGCC AAGCCAGTGT CCATACAGGA TAGAAGGGCG 
GTGGGGGGTT CTAGCACCTC TACCCCCTCC CACCCCCCAC CGCCTTACGG CGCCCACCAC 
ACGACCGCGG TGCCGGCGGA GCGGCACGTC CTGAGGCCCA CACGTCTGCC TCTGGGCAGG 
AGGAGTTTCC ACGGCCCGCT CCCGGTGACT GAAGCCTCCA CCTCAGTGGC CCCCCCCAGC 
TACTCTGAGG GTCCTTCCAG TCCTGGGGAG TACATCAACA TTGAGTTTGG GGATCAGTTT 
CCTCACCAAC AGCAGCCTCC TGCCTATCCT CTCTCTGCCC CAGATGAAGA GACCCCCCTT 
GGACCCTGCT GTTCGCCCCC CCAACCCCAG GACTACATGA GTGTTGAGGT GGGGGGTCTG 
GGAAAGAACC AGTCGCCCAG GCCTAGCCTT GTTGCCCCCT GGAACCCCCC CAGCTATATC 
AGACCTTTAG CTAGTAACCC TGGGGCGCAT GATGGGGGGC ACTGGAAGTC AGTGGGAGAA 
GATTACACTG ACATGACCTT TAACCTCAGT GGGGGTGGGA AAGCTCAGAG CACTACAGCT 
ATGCTGCAGC ATCTCTGCGT GATGGAGGGG CATTACAAAC ACACCTTCTC CTCACCCGTT 
TCTCCCCCCT GCCTGAACCG GCCACCCACG CAGCAGTCGG AGCCCAAAGT GGTACGAGCT 
GACCCCCAAG GCAGAAGAAG ACACAGTTCA GAGACGTTCT CTTCCAGCTC CACCCCTGCA 
GGAGAAGGGC TCAGCTCCTC CCCCCCCAAC TCCTCACAGG CTGACCCCAC AGCCCACTGT 
GAGTCTTACC TGGTGGGGGG ACAAGCTTCC AGATGGGCCA ACTCAGCATC CTTTGACTGT 
GTGTGGATGT CTGTGGAGGG GGTCAGAGAC TCGCCAGCTC CCCATGACTC ACCAGGAGCC 
ACAGAGGCCC CGCCCCCTTT CTCTGCCGCA GAGGCCGGAT CCGGCAGAAT GTGCAGGAAC 
GCGTCTGCCG GCTTCCAGAA CGGACTCAAC TACATCGCCT TGGAGCTAAG GGAGGAAGGG 
GTGAACACGG AGGCCGTGGC GTCTGGAGCG ACCGGCACCA ACGGGGACAC AGCTGGGGAG 
GGAACGGTGC CTCCGCCTGA GAACGGAGCC TATGCCAGTA TAGACTTCAC CAAAACGGAC 
GGAGTCGCAG CTGCCAAGGG GGGACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101169068 ( XM_023965868.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023821636.1 Oryzias latipes insulin receptor substrate 2-B (LOC101169068), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OJa74014
Clone ID Related Accession (Same CDS sequence)	XM_023965868.1 , XM_020711741.1
Accession Version	XM_023965868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	insulin receptor substrate 2-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCTAA CGAACCACCC GGAAGGGGGG GGCGCCGTGA TGATGGTGGA GGCCCCGCAG 
GGGGAGCCGG TGAGGAGAGC CGCGGTCACC AACGGAGCTG GCTCGGTGCG GGAACCCCCG 
TCCCCGGTAA TCGGCACCGG CTCCCGTTTC CACCCCCACC ACCACAGCCT GCAGCAGCCC 
TCCCGGGAGC CGCAGTTTCA TCTCTTTGGG GAGAAGAGCA CCGAGTCTCC GGGCCGGAGA 
GCCTGCCCAT CGCTCGGCCA GCTCCCCGCC GCCGAGGAGC CCCCCGCGGG CGGCGGTGCC 
CATGTGTTGG CGGCCGTGCA CGTCGCGAGC GCCTCTGACG CGGTGGAAGA CATCCGGAAA 
TGCGGATACC TGAGGAAGCA GAAACACGGA CACAAGCGCT TCTTCGTGCT CCGAGCCGCC 
GGGCCGCTCG GACCCAGCCG CCTGGAGTAC TACGACAGCG AGAAGAAGTT CCGGAGCAGC 
CTCCGCCACG CTGCGGGGGC CCCCGGAGCC GCCGCGGCCG CGCCTTCCCC TCCGAAAAGA 
GTGATCTTCC TGTCCCAGTG CTTCACGGTC AACAAGCGGG CGGACTCCAA GAACAAATAC 
CTGATCGCGC TCTACACCAA AGACGAGTAC TTCGCCATCG TGGCCGAGAG CGAGCAGGAG 
CAGGAGGACT GGTACGTGGC CGTCAGCGCT CTGATGAGTG AAGGCAAGCG TGGCCATGTG 
GACTCTGATG ACCTGGATGA TGGGTACGGT ACCGTCAGTC CCGGTACCGT GTTCAAAGAG 
GTGTGGCAGG TCAATGTGAA ACCTAAAGGA CTAGGTCAGA CCAAAAACCT GACCGGCGTG 
TACCGTCTGT GCCTCTCTGC CAAAACCGTT CACCTGGTGA AGCTGAACTC CGACAACCCC 
TGTGTCAACC TGCAGCTGAT GAACATCCGG CGCTGTGGAC ATTCAGAGAG CTTCTTCTTC 
ATCGAGGTGG GCCGCTCCTC TTCTATCGGG CCCGGGGAGA TCTGGATGCA GGTGGATGAC 
TCTGTTGTGG CCCAGAGCAT GCACGAGACC ATCCTGGAGA CCATGAAAGC CCTCAAGGCT 
TTCGCAGAGT TCCGGCCCAG GAGCAAGAGT CAGTCTTCGA GCTCCAACCC CATGCCCTTC 
ATCACCACGC GCCGCCACCT GGGCAACCTG CCTCCAAGCC AGACTGGGCT GCAGCGCCGA 
TCCAGAACGG AGTCGGTGGT CGGGACCCCT CCGTCCAGTA AGAGCTCAGG GGCGAGCGGT 
TATCGCTTCC GGACCTCCAG TGAGGGAGAG GGAACCATGA ACCGGCCGTT CCGCTCTGCC 
ACGGGGAGCC TGGTGCATCT GAACTCAACG CGGGCCCTCC ATGGGCGTCA GGACGCTACT 
GGCGGCGTCT CTGGAAGTGG GGGGGCTACA GGCAGTTCTG GCACCAGCAG CGGGCGCTAC 
ACGAAGGCCA TATCCGGGAC ATCCTCTTCC TACCACGCTC GCTCATCCTC CCTGCCTGTC 
TCCCACTTCC CCTCCACCAG CAGCCCGGTC AGCGTGTCCT CCAGCAGCGG GCATGGCTCC 
GTGTCGGACA CGCTCACCCG CCCATCCAGC GCCTCCATAT GCGGCTCGCC GTCTGACGGG 
GGCTTCAACT CTTCTGACGA GTACGGATCC AGTCCGGGGG ACTTTCGGTA CTTTAGGGTG 
AGGAGTAACA CGCCCGACTC TCTGGGCAAC ACCCCCCCAA TCAGAGAGGA GAGCTGTCTT 
AATGATTACA TGGCCATGGG CTGGAACCGA GATGTTTTTG GTGGTGCGGG GGACGCTTCC 
CGTGAGGAGA GCACCTCAAC AGCCGATGAC GACCGCTTGT CCTCCTCATC TCTGAGGAGG 
AGGACGCACT CTTTCTCCAG ATCTGCAGGG GGGGCAACTG GAGGCTGTGG TGTGGCGGTT 
TATCAGAAGA TGACCCAGAC CAACCTCTCA TTGGATGAGG GCTCGGATGT GGCGCTGCCG 
TTTGGTGCCG GGCTGCTCCG GGGAGGGCCC TCCTCCTCCA CTTCCTCGCT CCGCTCTGAT 
TACAGCTCCT GTTCAGAACA CAGCCAGCAG AGCCGGCCCT CCACCCTCCC CAGGACTGAG 
GCTGCTGCTG AGCGCCCACC CCTCTGTGCC CAGGAGGATA GCGGCTACAT GCCCATGATG 
TGTGGCGTCG CTGCATCGCC GCGGGACACC CCCCCTGACT ACATGCCTAT GCAACCCAGC 
TCCTACCCCC AACACAACTC CCATTCCCCC CAGTTTCACA GCCGTTCACC CCACCGACCC 
ACACAGCCGC AGGCCTCCAC AGACGCTCAA GGCTACATGA TGATGCTCCC TGGGGGCGGG 
GGCTGCACGT CTTCAGTGCT GCAGGCCTCA CCCAGTCCTC CCAGTAGTTC CAGTTCATCT 
GCTGCTACTG GAAGCTGCAG GGGTGGGGGG GGCGAGAATG GAGAGTACAT GGACATGTCT 
TACAGCAGCG GGCACAGGCT CTCAAGTGCC ACAGGTGGCC CCCCTGAAGG AGCCCCCAAG 
TCTTACAGCC CCTACTTCTC CCTCCCACGC TCCTATAAGA CCCCCACCAG AGAGAGGGAT 
GGAGAGTATG TACCCATGAG CCCCCCTGCC AAGCCAGTGT CCATACAGGA TAGAAGGGCG 
GTGGGGGGTT CTAGCACCTC TACCCCCTCC CACCCCCCAC CGCCTTACGG CGCCCACCAC 
ACGACCGCGG TGCCGGCGGA GCGGCACGTC CTGAGGCCCA CACGTCTGCC TCTGGGCAGG 
AGGAGTTTCC ACGGCCCGCT CCCGGTGACT GAAGCCTCCA CCTCAGTGGC CCCCCCCAGC 
TACTCTGAGG GTCCTTCCAG TCCTGGGGAG TACATCAACA TTGAGTTTGG GGATCAGTTT 
CCTCACCAAC AGCAGCCTCC TGCCTATCCT CTCTCTGCCC CAGATGAAGA GACCCCCCTT 
GGACCCTGCT GTTCGCCCCC CCAACCCCAG GACTACATGA GTGTTGAGGT GGGGGGTCTG 
GGAAAGAACC AGTCGCCCAG GCCTAGCCTT GTTGCCCCCT GGAACCCCCC CAGCTATATC 
AGACCTTTAG CTAGTAACCC TGGGGCGCAT GATGGGGGGC ACTGGAAGTC AGTGGGAGAA 
GATTACACTG ACATGACCTT TAACCTCAGT GGGGGTGGGA AAGCTCAGAG CACTACAGCT 
ATGCTGCAGC ATCTCTGCGT GATGGAGGGG CATTACAAAC ACACCTTCTC CTCACCCGTT 
TCTCCCCCCT GCCTGAACCG GCCACCCACG CAGCAGTCGG AGCCCAAAGT GGTACGAGCT 
GACCCCCAAG GCAGAAGAAG ACACAGTTCA GAGACGTTCT CTTCCAGCTC CACCCCTGCA 
GGAGAAGGGC TCAGCTCCTC CCCCCCCAAC TCCTCACAGG CTGACCCCAC AGCCCACTGT 
GAGTCTTACC TGGTGGGGGG ACAAGCTTCC AGATGGGCCA ACTCAGCATC CTTTGACTGT 
GTGTGGATGT CTGTGGAGGG GGTCAGAGAC TCGCCAGCTC CCCATGACTC ACCAGGAGCC 
ACAGAGGCCC CGCCCCCTTT CTCTGCCGCA GAGGCCGGAT CCGGCAGAAT GTGCAGGAAC 
GCGTCTGCCG GCTTCCAGAA CGGACTCAAC TACATCGCCT TGGAGCTAAG GGAGGAAGGG 
GTGAACACGG AGGCCGTGGC GTCTGGAGCG ACCGGCACCA ACGGGGACAC AGCTGGGGAG 
GGAACGGTGC CTCCGCCTGA GAACGGAGCC TATGCCAGTA TAGACTTCAC CAAAACGGAC 
GGAGTCGCAG CTGCCAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023821636.1
CDS	514..4317
Translation	MDLTNHPEGGGAVMMVEAPQGEPVRRAAVTNGAGSVREPPSPVIGTGSRFHPHHHSLQQPSREPQFHLFGEKSTESPGRRACPSLGQLPAAEEPPAGGGAHVLAAVHVASASDAVEDIRKCGYLRKQKHGHKRFFVLRAAGPLGPSRLEYYDSEKKFRSSLRHAAGAPGAAAAAPSPPKRVIFLSQCFTVNKRADSKNKYLIALYTKDEYFAIVAESEQEQEDWYVAVSALMSEGKRGHVDSDDLDDGYGTVSPGTVFKEVWQVNVKPKGLGQTKNLTGVYRLCLSAKTVHLVKLNSDNPCVNLQLMNIRRCGHSESFFFIEVGRSSSIGPGEIWMQVDDSVVAQSMHETILETMKALKAFAEFRPRSKSQSSSSNPMPFITTRRHLGNLPPSQTGLQRRSRTESVVGTPPSSKSSGASGYRFRTSSEGEGTMNRPFRSATGSLVHLNSTRALHGRQDATGGVSGSGGATGSSGTSSGRYTKAISGTSSSYHARSSSLPVSHFPSTSSPVSVSSSSGHGSVSDTLTRPSSASICGSPSDGGFNSSDEYGSSPGDFRYFRVRSNTPDSLGNTPPIREESCLNDYMAMGWNRDVFGGAGDASREESTSTADDDRLSSSSLRRRTHSFSRSAGGATGGCGVAVYQKMTQTNLSLDEGSDVALPFGAGLLRGGPSSSTSSLRSDYSSCSEHSQQSRPSTLPRTEAAAERPPLCAQEDSGYMPMMCGVAASPRDTPPDYMPMQPSSYPQHNSHSPQFHSRSPHRPTQPQASTDAQGYMMMLPGGGGCTSSVLQASPSPPSSSSSSAATGSCRGGGGENGEYMDMSYSSGHRLSSATGGPPEGAPKSYSPYFSLPRSYKTPTRERDGEYVPMSPPAKPVSIQDRRAVGGSSTSTPSHPPPPYGAHHTTAVPAERHVLRPTRLPLGRRSFHGPLPVTEASTSVAPPSYSEGPSSPGEYINIEFGDQFPHQQQPPAYPLSAPDEETPLGPCCSPPQPQDYMSVEVGGLGKNQSPRPSLVAPWNPPSYIRPLASNPGAHDGGHWKSVGEDYTDMTFNLSGGGKAQSTTAMLQHLCVMEGHYKHTFSSPVSPPCLNRPPTQQSEPKVVRADPQGRRRHSSETFSSSSTPAGEGLSSSPPNSSQADPTAHCESYLVGGQASRWANSASFDCVWMSVEGVRDSPAPHDSPGATEAPPPFSAAEAGSGRMCRNASAGFQNGLNYIALELREEGVNTEAVASGATGTNGDTAGEGTVPPPENGAYASIDFTKTDGVAAAKD

Target ORF information:

RefSeq Version	XM_023965868.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes insulin receptor substrate 2-B (LOC101169068), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023965868.1

ORF Insert Sequence:

ATGGATCTAA CGAACCACCC GGAAGGGGGG GGCGCCGTGA TGATGGTGGA GGCCCCGCAG 
GGGGAGCCGG TGAGGAGAGC CGCGGTCACC AACGGAGCTG GCTCGGTGCG GGAACCCCCG 
TCCCCGGTAA TCGGCACCGG CTCCCGTTTC CACCCCCACC ACCACAGCCT GCAGCAGCCC 
TCCCGGGAGC CGCAGTTTCA TCTCTTTGGG GAGAAGAGCA CCGAGTCTCC GGGCCGGAGA 
GCCTGCCCAT CGCTCGGCCA GCTCCCCGCC GCCGAGGAGC CCCCCGCGGG CGGCGGTGCC 
CATGTGTTGG CGGCCGTGCA CGTCGCGAGC GCCTCTGACG CGGTGGAAGA CATCCGGAAA 
TGCGGATACC TGAGGAAGCA GAAACACGGA CACAAGCGCT TCTTCGTGCT CCGAGCCGCC 
GGGCCGCTCG GACCCAGCCG CCTGGAGTAC TACGACAGCG AGAAGAAGTT CCGGAGCAGC 
CTCCGCCACG CTGCGGGGGC CCCCGGAGCC GCCGCGGCCG CGCCTTCCCC TCCGAAAAGA 
GTGATCTTCC TGTCCCAGTG CTTCACGGTC AACAAGCGGG CGGACTCCAA GAACAAATAC 
CTGATCGCGC TCTACACCAA AGACGAGTAC TTCGCCATCG TGGCCGAGAG CGAGCAGGAG 
CAGGAGGACT GGTACGTGGC CGTCAGCGCT CTGATGAGTG AAGGCAAGCG TGGCCATGTG 
GACTCTGATG ACCTGGATGA TGGGTACGGT ACCGTCAGTC CCGGTACCGT GTTCAAAGAG 
GTGTGGCAGG TCAATGTGAA ACCTAAAGGA CTAGGTCAGA CCAAAAACCT GACCGGCGTG 
TACCGTCTGT GCCTCTCTGC CAAAACCGTT CACCTGGTGA AGCTGAACTC CGACAACCCC 
TGTGTCAACC TGCAGCTGAT GAACATCCGG CGCTGTGGAC ATTCAGAGAG CTTCTTCTTC 
ATCGAGGTGG GCCGCTCCTC TTCTATCGGG CCCGGGGAGA TCTGGATGCA GGTGGATGAC 
TCTGTTGTGG CCCAGAGCAT GCACGAGACC ATCCTGGAGA CCATGAAAGC CCTCAAGGCT 
TTCGCAGAGT TCCGGCCCAG GAGCAAGAGT CAGTCTTCGA GCTCCAACCC CATGCCCTTC 
ATCACCACGC GCCGCCACCT GGGCAACCTG CCTCCAAGCC AGACTGGGCT GCAGCGCCGA 
TCCAGAACGG AGTCGGTGGT CGGGACCCCT CCGTCCAGTA AGAGCTCAGG GGCGAGCGGT 
TATCGCTTCC GGACCTCCAG TGAGGGAGAG GGAACCATGA ACCGGCCGTT CCGCTCTGCC 
ACGGGGAGCC TGGTGCATCT GAACTCAACG CGGGCCCTCC ATGGGCGTCA GGACGCTACT 
GGCGGCGTCT CTGGAAGTGG GGGGGCTACA GGCAGTTCTG GCACCAGCAG CGGGCGCTAC 
ACGAAGGCCA TATCCGGGAC ATCCTCTTCC TACCACGCTC GCTCATCCTC CCTGCCTGTC 
TCCCACTTCC CCTCCACCAG CAGCCCGGTC AGCGTGTCCT CCAGCAGCGG GCATGGCTCC 
GTGTCGGACA CGCTCACCCG CCCATCCAGC GCCTCCATAT GCGGCTCGCC GTCTGACGGG 
GGCTTCAACT CTTCTGACGA GTACGGATCC AGTCCGGGGG ACTTTCGGTA CTTTAGGGTG 
AGGAGTAACA CGCCCGACTC TCTGGGCAAC ACCCCCCCAA TCAGAGAGGA GAGCTGTCTT 
AATGATTACA TGGCCATGGG CTGGAACCGA GATGTTTTTG GTGGTGCGGG GGACGCTTCC 
CGTGAGGAGA GCACCTCAAC AGCCGATGAC GACCGCTTGT CCTCCTCATC TCTGAGGAGG 
AGGACGCACT CTTTCTCCAG ATCTGCAGGG GGGGCAACTG GAGGCTGTGG TGTGGCGGTT 
TATCAGAAGA TGACCCAGAC CAACCTCTCA TTGGATGAGG GCTCGGATGT GGCGCTGCCG 
TTTGGTGCCG GGCTGCTCCG GGGAGGGCCC TCCTCCTCCA CTTCCTCGCT CCGCTCTGAT 
TACAGCTCCT GTTCAGAACA CAGCCAGCAG AGCCGGCCCT CCACCCTCCC CAGGACTGAG 
GCTGCTGCTG AGCGCCCACC CCTCTGTGCC CAGGAGGATA GCGGCTACAT GCCCATGATG 
TGTGGCGTCG CTGCATCGCC GCGGGACACC CCCCCTGACT ACATGCCTAT GCAACCCAGC 
TCCTACCCCC AACACAACTC CCATTCCCCC CAGTTTCACA GCCGTTCACC CCACCGACCC 
ACACAGCCGC AGGCCTCCAC AGACGCTCAA GGCTACATGA TGATGCTCCC TGGGGGCGGG 
GGCTGCACGT CTTCAGTGCT GCAGGCCTCA CCCAGTCCTC CCAGTAGTTC CAGTTCATCT 
GCTGCTACTG GAAGCTGCAG GGGTGGGGGG GGCGAGAATG GAGAGTACAT GGACATGTCT 
TACAGCAGCG GGCACAGGCT CTCAAGTGCC ACAGGTGGCC CCCCTGAAGG AGCCCCCAAG 
TCTTACAGCC CCTACTTCTC CCTCCCACGC TCCTATAAGA CCCCCACCAG AGAGAGGGAT 
GGAGAGTATG TACCCATGAG CCCCCCTGCC AAGCCAGTGT CCATACAGGA TAGAAGGGCG 
GTGGGGGGTT CTAGCACCTC TACCCCCTCC CACCCCCCAC CGCCTTACGG CGCCCACCAC 
ACGACCGCGG TGCCGGCGGA GCGGCACGTC CTGAGGCCCA CACGTCTGCC TCTGGGCAGG 
AGGAGTTTCC ACGGCCCGCT CCCGGTGACT GAAGCCTCCA CCTCAGTGGC CCCCCCCAGC 
TACTCTGAGG GTCCTTCCAG TCCTGGGGAG TACATCAACA TTGAGTTTGG GGATCAGTTT 
CCTCACCAAC AGCAGCCTCC TGCCTATCCT CTCTCTGCCC CAGATGAAGA GACCCCCCTT 
GGACCCTGCT GTTCGCCCCC CCAACCCCAG GACTACATGA GTGTTGAGGT GGGGGGTCTG 
GGAAAGAACC AGTCGCCCAG GCCTAGCCTT GTTGCCCCCT GGAACCCCCC CAGCTATATC 
AGACCTTTAG CTAGTAACCC TGGGGCGCAT GATGGGGGGC ACTGGAAGTC AGTGGGAGAA 
GATTACACTG ACATGACCTT TAACCTCAGT GGGGGTGGGA AAGCTCAGAG CACTACAGCT 
ATGCTGCAGC ATCTCTGCGT GATGGAGGGG CATTACAAAC ACACCTTCTC CTCACCCGTT 
TCTCCCCCCT GCCTGAACCG GCCACCCACG CAGCAGTCGG AGCCCAAAGT GGTACGAGCT 
GACCCCCAAG GCAGAAGAAG ACACAGTTCA GAGACGTTCT CTTCCAGCTC CACCCCTGCA 
GGAGAAGGGC TCAGCTCCTC CCCCCCCAAC TCCTCACAGG CTGACCCCAC AGCCCACTGT 
GAGTCTTACC TGGTGGGGGG ACAAGCTTCC AGATGGGCCA ACTCAGCATC CTTTGACTGT 
GTGTGGATGT CTGTGGAGGG GGTCAGAGAC TCGCCAGCTC CCCATGACTC ACCAGGAGCC 
ACAGAGGCCC CGCCCCCTTT CTCTGCCGCA GAGGCCGGAT CCGGCAGAAT GTGCAGGAAC 
GCGTCTGCCG GCTTCCAGAA CGGACTCAAC TACATCGCCT TGGAGCTAAG GGAGGAAGGG 
GTGAACACGG AGGCCGTGGC GTCTGGAGCG ACCGGCACCA ACGGGGACAC AGCTGGGGAG 
GGAACGGTGC CTCCGCCTGA GAACGGAGCC TATGCCAGTA TAGACTTCAC CAAAACGGAC 
GGAGTCGCAG CTGCCAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101169068 ( XM_020711741.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020567400.1 Oryzias latipes insulin receptor substrate 2-B-like (LOC101169068), mRNA.

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CloneID	OJa74014
Clone ID Related Accession (Same CDS sequence)	XM_023965868.1 , XM_020711741.1
Accession Version	XM_020711741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	insulin receptor substrate 2-B-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.1) and transcript sequence (GFIO01028449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 407 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGATCTAA CGAACCACCC GGAAGGGGGG GGCGCCGTGA TGATGGTGGA GGCCCCGCAG 
GGGGAGCCGG TGAGGAGAGC CGCGGTCACC AACGGAGCTG GCTCGGTGCG GGAACCCCCG 
TCCCCGGTAA TCGGCACCGG CTCCCGTTTC CACCCCCACC ACCACAGCCT GCAGCAGCCC 
TCCCGGGAGC CGCAGTTTCA TCTCTTTGGG GAGAAGAGCA CCGAGTCTCC GGGCCGGAGA 
GCCTGCCCAT CGCTCGGCCA GCTCCCCGCC GCCGAGGAGC CCCCCGCGGG CGGCGGTGCC 
CATGTGTTGG CGGCCGTGCA CGTCGCGAGC GCCTCTGACG CGGTGGAAGA CATCCGGAAA 
TGCGGATACC TGAGGAAGCA GAAACACGGA CACAAGCGCT TCTTCGTGCT CCGAGCCGCC 
GGGCCGCTCG GACCCAGCCG CCTGGAGTAC TACGACAGCG AGAAGAAGTT CCGGAGCAGC 
CTCCGCCACG CTGCGGGGGC CCCCGGAGCC GCCGCGGCCG CGCCTTCCCC TCCGAAAAGA 
GTGATCTTCC TGTCCCAGTG CTTCACGGTC AACAAGCGGG CGGACTCCAA GAACAAATAC 
CTGATCGCGC TCTACACCAA AGACGAGTAC TTCGCCATCG TGGCCGAGAG CGAGCAGGAG 
CAGGAGGACT GGTACGTGGC CGTCAGCGCT CTGATGAGTG AAGGCAAGCG TGGCCATGTG 
GACTCTGATG ACCTGGATGA TGGGTACGGT ACCGTCAGTC CCGGTACCGT GTTCAAAGAG 
GTGTGGCAGG TCAATGTGAA ACCTAAAGGA CTAGGTCAGA CCAAAAACCT GACCGGCGTG 
TACCGTCTGT GCCTCTCTGC CAAAACCGTT CACCTGGTGA AGCTGAACTC CGACAACCCC 
TGTGTCAACC TGCAGCTGAT GAACATCCGG CGCTGTGGAC ATTCAGAGAG CTTCTTCTTC 
ATCGAGGTGG GCCGCTCCTC TTCTATCGGG CCCGGGGAGA TCTGGATGCA GGTGGATGAC 
TCTGTTGTGG CCCAGAGCAT GCACGAGACC ATCCTGGAGA CCATGAAAGC CCTCAAGGCT 
TTCGCAGAGT TCCGGCCCAG GAGCAAGAGT CAGTCTTCGA GCTCCAACCC CATGCCCTTC 
ATCACCACGC GCCGCCACCT GGGCAACCTG CCTCCAAGCC AGACTGGGCT GCAGCGCCGA 
TCCAGAACGG AGTCGGTGGT CGGGACCCCT CCGTCCAGTA AGAGCTCAGG GGCGAGCGGT 
TATCGCTTCC GGACCTCCAG TGAGGGAGAG GGAACCATGA ACCGGCCGTT CCGCTCTGCC 
ACGGGGAGCC TGGTGCATCT GAACTCAACG CGGGCCCTCC ATGGGCGTCA GGACGCTACT 
GGCGGCGTCT CTGGAAGTGG GGGGGCTACA GGCAGTTCTG GCACCAGCAG CGGGCGCTAC 
ACGAAGGCCA TATCCGGGAC ATCCTCTTCC TACCACGCTC GCTCATCCTC CCTGCCTGTC 
TCCCACTTCC CCTCCACCAG CAGCCCGGTC AGCGTGTCCT CCAGCAGCGG GCATGGCTCC 
GTGTCGGACA CGCTCACCCG CCCATCCAGC GCCTCCATAT GCGGCTCGCC GTCTGACGGG 
GGCTTCAACT CTTCTGACGA GTACGGATCC AGTCCGGGGG ACTTTCGGTA CTTTAGGGTG 
AGGAGTAACA CGCCCGACTC TCTGGGCAAC ACCCCCCCAA TCAGAGAGGA GAGCTGTCTT 
AATGATTACA TGGCCATGGG CTGGAACCGA GATGTTTTTG GTGGTGCGGG GGACGCTTCC 
CGTGAGGAGA GCACCTCAAC AGCCGATGAC GACCGCTTGT CCTCCTCATC TCTGAGGAGG 
AGGACGCACT CTTTCTCCAG ATCTGCAGGG GGGGCAACTG GAGGCTGTGG TGTGGCGGTT 
TATCAGAAGA TGACCCAGAC CAACCTCTCA TTGGATGAGG GCTCGGATGT GGCGCTGCCG 
TTTGGTGCCG GGCTGCTCCG GGGAGGGCCC TCCTCCTCCA CTTCCTCGCT CCGCTCTGAT 
TACAGCTCCT GTTCAGAACA CAGCCAGCAG AGCCGGCCCT CCACCCTCCC CAGGACTGAG 
GCTGCTGCTG AGCGCCCACC CCTCTGTGCC CAGGAGGATA GCGGCTACAT GCCCATGATG 
TGTGGCGTCG CTGCATCGCC GCGGGACACC CCCCCTGACT ACATGCCTAT GCAACCCAGC 
TCCTACCCCC AACACAACTC CCATTCCCCC CAGTTTCACA GCCGTTCACC CCACCGACCC 
ACACAGCCGC AGGCCTCCAC AGACGCTCAA GGCTACATGA TGATGCTCCC TGGGGGCGGG 
GGCTGCACGT CTTCAGTGCT GCAGGCCTCA CCCAGTCCTC CCAGTAGTTC CAGTTCATCT 
GCTGCTACTG GAAGCTGCAG GGGTGGGGGG GGCGAGAATG GAGAGTACAT GGACATGTCT 
TACAGCAGCG GGCACAGGCT CTCAAGTGCC ACAGGTGGCC CCCCTGAAGG AGCCCCCAAG 
TCTTACAGCC CCTACTTCTC CCTCCCACGC TCCTATAAGA CCCCCACCAG AGAGAGGGAT 
GGAGAGTATG TACCCATGAG CCCCCCTGCC AAGCCAGTGT CCATACAGGA TAGAAGGGCG 
GTGGGGGGTT CTAGCACCTC TACCCCCTCC CACCCCCCAC CGCCTTACGG CGCCCACCAC 
ACGACCGCGG TGCCGGCGGA GCGGCACGTC CTGAGGCCCA CACGTCTGCC TCTGGGCAGG 
AGGAGTTTCC ACGGCCCGCT CCCGGTGACT GAAGCCTCCA CCTCAGTGGC CCCCCCCAGC 
TACTCTGAGG GTCCTTCCAG TCCTGGGGAG TACATCAACA TTGAGTTTGG GGATCAGTTT 
CCTCACCAAC AGCAGCCTCC TGCCTATCCT CTCTCTGCCC CAGATGAAGA GACCCCCCTT 
GGACCCTGCT GTTCGCCCCC CCAACCCCAG GACTACATGA GTGTTGAGGT GGGGGGTCTG 
GGAAAGAACC AGTCGCCCAG GCCTAGCCTT GTTGCCCCCT GGAACCCCCC CAGCTATATC 
AGACCTTTAG CTAGTAACCC TGGGGCGCAT GATGGGGGGC ACTGGAAGTC AGTGGGAGAA 
GATTACACTG ACATGACCTT TAACCTCAGT GGGGGTGGGA AAGCTCAGAG CACTACAGCT 
ATGCTGCAGC ATCTCTGCGT GATGGAGGGG CATTACAAAC ACACCTTCTC CTCACCCGTT 
TCTCCCCCCT GCCTGAACCG GCCACCCACG CAGCAGTCGG AGCCCAAAGT GGTACGAGCT 
GACCCCCAAG GCAGAAGAAG ACACAGTTCA GAGACGTTCT CTTCCAGCTC CACCCCTGCA 
GGAGAAGGGC TCAGCTCCTC CCCCCCCAAC TCCTCACAGG CTGACCCCAC AGCCCACTGT 
GAGTCTTACC TGGTGGGGGG ACAAGCTTCC AGATGGGCCA ACTCAGCATC CTTTGACTGT 
GTGTGGATGT CTGTGGAGGG GGTCAGAGAC TCGCCAGCTC CCCATGACTC ACCAGGAGCC 
ACAGAGGCCC CGCCCCCTTT CTCTGCCGCA GAGGCCGGAT CCGGCAGAAT GTGCAGGAAC 
GCGTCTGCCG GCTTCCAGAA CGGACTCAAC TACATCGCCT TGGAGCTAAG GGAGGAAGGG 
GTGAACACGG AGGCCGTGGC GTCTGGAGCG ACCGGCACCA ACGGGGACAC AGCTGGGGAG 
GGAACGGTGC CTCCGCCTGA GAACGGAGCC TATGCCAGTA TAGACTTCAC CAAAACGGAC 
GGAGTCGCAG CTGCCAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020567400.1
CDS	528..4331
Translation	MDLTNHPEGGGAVMMVEAPQGEPVRRAAVTNGAGSVREPPSPVIGTGSRFHPHHHSLQQPSREPQFHLFGEKSTESPGRRACPSLGQLPAAEEPPAGGGAHVLAAVHVASASDAVEDIRKCGYLRKQKHGHKRFFVLRAAGPLGPSRLEYYDSEKKFRSSLRHAAGAPGAAAAAPSPPKRVIFLSQCFTVNKRADSKNKYLIALYTKDEYFAIVAESEQEQEDWYVAVSALMSEGKRGHVDSDDLDDGYGTVSPGTVFKEVWQVNVKPKGLGQTKNLTGVYRLCLSAKTVHLVKLNSDNPCVNLQLMNIRRCGHSESFFFIEVGRSSSIGPGEIWMQVDDSVVAQSMHETILETMKALKAFAEFRPRSKSQSSSSNPMPFITTRRHLGNLPPSQTGLQRRSRTESVVGTPPSSKSSGASGYRFRTSSEGEGTMNRPFRSATGSLVHLNSTRALHGRQDATGGVSGSGGATGSSGTSSGRYTKAISGTSSSYHARSSSLPVSHFPSTSSPVSVSSSSGHGSVSDTLTRPSSASICGSPSDGGFNSSDEYGSSPGDFRYFRVRSNTPDSLGNTPPIREESCLNDYMAMGWNRDVFGGAGDASREESTSTADDDRLSSSSLRRRTHSFSRSAGGATGGCGVAVYQKMTQTNLSLDEGSDVALPFGAGLLRGGPSSSTSSLRSDYSSCSEHSQQSRPSTLPRTEAAAERPPLCAQEDSGYMPMMCGVAASPRDTPPDYMPMQPSSYPQHNSHSPQFHSRSPHRPTQPQASTDAQGYMMMLPGGGGCTSSVLQASPSPPSSSSSSAATGSCRGGGGENGEYMDMSYSSGHRLSSATGGPPEGAPKSYSPYFSLPRSYKTPTRERDGEYVPMSPPAKPVSIQDRRAVGGSSTSTPSHPPPPYGAHHTTAVPAERHVLRPTRLPLGRRSFHGPLPVTEASTSVAPPSYSEGPSSPGEYINIEFGDQFPHQQQPPAYPLSAPDEETPLGPCCSPPQPQDYMSVEVGGLGKNQSPRPSLVAPWNPPSYIRPLASNPGAHDGGHWKSVGEDYTDMTFNLSGGGKAQSTTAMLQHLCVMEGHYKHTFSSPVSPPCLNRPPTQQSEPKVVRADPQGRRRHSSETFSSSSTPAGEGLSSSPPNSSQADPTAHCESYLVGGQASRWANSASFDCVWMSVEGVRDSPAPHDSPGATEAPPPFSAAEAGSGRMCRNASAGFQNGLNYIALELREEGVNTEAVASGATGTNGDTAGEGTVPPPENGAYASIDFTKTDGVAAAKD

Target ORF information:

RefSeq Version	XM_020711741.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes insulin receptor substrate 2-B-like (LOC101169068), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020711741.1

ORF Insert Sequence:

ATGGATCTAA CGAACCACCC GGAAGGGGGG GGCGCCGTGA TGATGGTGGA GGCCCCGCAG 
GGGGAGCCGG TGAGGAGAGC CGCGGTCACC AACGGAGCTG GCTCGGTGCG GGAACCCCCG 
TCCCCGGTAA TCGGCACCGG CTCCCGTTTC CACCCCCACC ACCACAGCCT GCAGCAGCCC 
TCCCGGGAGC CGCAGTTTCA TCTCTTTGGG GAGAAGAGCA CCGAGTCTCC GGGCCGGAGA 
GCCTGCCCAT CGCTCGGCCA GCTCCCCGCC GCCGAGGAGC CCCCCGCGGG CGGCGGTGCC 
CATGTGTTGG CGGCCGTGCA CGTCGCGAGC GCCTCTGACG CGGTGGAAGA CATCCGGAAA 
TGCGGATACC TGAGGAAGCA GAAACACGGA CACAAGCGCT TCTTCGTGCT CCGAGCCGCC 
GGGCCGCTCG GACCCAGCCG CCTGGAGTAC TACGACAGCG AGAAGAAGTT CCGGAGCAGC 
CTCCGCCACG CTGCGGGGGC CCCCGGAGCC GCCGCGGCCG CGCCTTCCCC TCCGAAAAGA 
GTGATCTTCC TGTCCCAGTG CTTCACGGTC AACAAGCGGG CGGACTCCAA GAACAAATAC 
CTGATCGCGC TCTACACCAA AGACGAGTAC TTCGCCATCG TGGCCGAGAG CGAGCAGGAG 
CAGGAGGACT GGTACGTGGC CGTCAGCGCT CTGATGAGTG AAGGCAAGCG TGGCCATGTG 
GACTCTGATG ACCTGGATGA TGGGTACGGT ACCGTCAGTC CCGGTACCGT GTTCAAAGAG 
GTGTGGCAGG TCAATGTGAA ACCTAAAGGA CTAGGTCAGA CCAAAAACCT GACCGGCGTG 
TACCGTCTGT GCCTCTCTGC CAAAACCGTT CACCTGGTGA AGCTGAACTC CGACAACCCC 
TGTGTCAACC TGCAGCTGAT GAACATCCGG CGCTGTGGAC ATTCAGAGAG CTTCTTCTTC 
ATCGAGGTGG GCCGCTCCTC TTCTATCGGG CCCGGGGAGA TCTGGATGCA GGTGGATGAC 
TCTGTTGTGG CCCAGAGCAT GCACGAGACC ATCCTGGAGA CCATGAAAGC CCTCAAGGCT 
TTCGCAGAGT TCCGGCCCAG GAGCAAGAGT CAGTCTTCGA GCTCCAACCC CATGCCCTTC 
ATCACCACGC GCCGCCACCT GGGCAACCTG CCTCCAAGCC AGACTGGGCT GCAGCGCCGA 
TCCAGAACGG AGTCGGTGGT CGGGACCCCT CCGTCCAGTA AGAGCTCAGG GGCGAGCGGT 
TATCGCTTCC GGACCTCCAG TGAGGGAGAG GGAACCATGA ACCGGCCGTT CCGCTCTGCC 
ACGGGGAGCC TGGTGCATCT GAACTCAACG CGGGCCCTCC ATGGGCGTCA GGACGCTACT 
GGCGGCGTCT CTGGAAGTGG GGGGGCTACA GGCAGTTCTG GCACCAGCAG CGGGCGCTAC 
ACGAAGGCCA TATCCGGGAC ATCCTCTTCC TACCACGCTC GCTCATCCTC CCTGCCTGTC 
TCCCACTTCC CCTCCACCAG CAGCCCGGTC AGCGTGTCCT CCAGCAGCGG GCATGGCTCC 
GTGTCGGACA CGCTCACCCG CCCATCCAGC GCCTCCATAT GCGGCTCGCC GTCTGACGGG 
GGCTTCAACT CTTCTGACGA GTACGGATCC AGTCCGGGGG ACTTTCGGTA CTTTAGGGTG 
AGGAGTAACA CGCCCGACTC TCTGGGCAAC ACCCCCCCAA TCAGAGAGGA GAGCTGTCTT 
AATGATTACA TGGCCATGGG CTGGAACCGA GATGTTTTTG GTGGTGCGGG GGACGCTTCC 
CGTGAGGAGA GCACCTCAAC AGCCGATGAC GACCGCTTGT CCTCCTCATC TCTGAGGAGG 
AGGACGCACT CTTTCTCCAG ATCTGCAGGG GGGGCAACTG GAGGCTGTGG TGTGGCGGTT 
TATCAGAAGA TGACCCAGAC CAACCTCTCA TTGGATGAGG GCTCGGATGT GGCGCTGCCG 
TTTGGTGCCG GGCTGCTCCG GGGAGGGCCC TCCTCCTCCA CTTCCTCGCT CCGCTCTGAT 
TACAGCTCCT GTTCAGAACA CAGCCAGCAG AGCCGGCCCT CCACCCTCCC CAGGACTGAG 
GCTGCTGCTG AGCGCCCACC CCTCTGTGCC CAGGAGGATA GCGGCTACAT GCCCATGATG 
TGTGGCGTCG CTGCATCGCC GCGGGACACC CCCCCTGACT ACATGCCTAT GCAACCCAGC 
TCCTACCCCC AACACAACTC CCATTCCCCC CAGTTTCACA GCCGTTCACC CCACCGACCC 
ACACAGCCGC AGGCCTCCAC AGACGCTCAA GGCTACATGA TGATGCTCCC TGGGGGCGGG 
GGCTGCACGT CTTCAGTGCT GCAGGCCTCA CCCAGTCCTC CCAGTAGTTC CAGTTCATCT 
GCTGCTACTG GAAGCTGCAG GGGTGGGGGG GGCGAGAATG GAGAGTACAT GGACATGTCT 
TACAGCAGCG GGCACAGGCT CTCAAGTGCC ACAGGTGGCC CCCCTGAAGG AGCCCCCAAG 
TCTTACAGCC CCTACTTCTC CCTCCCACGC TCCTATAAGA CCCCCACCAG AGAGAGGGAT 
GGAGAGTATG TACCCATGAG CCCCCCTGCC AAGCCAGTGT CCATACAGGA TAGAAGGGCG 
GTGGGGGGTT CTAGCACCTC TACCCCCTCC CACCCCCCAC CGCCTTACGG CGCCCACCAC 
ACGACCGCGG TGCCGGCGGA GCGGCACGTC CTGAGGCCCA CACGTCTGCC TCTGGGCAGG 
AGGAGTTTCC ACGGCCCGCT CCCGGTGACT GAAGCCTCCA CCTCAGTGGC CCCCCCCAGC 
TACTCTGAGG GTCCTTCCAG TCCTGGGGAG TACATCAACA TTGAGTTTGG GGATCAGTTT 
CCTCACCAAC AGCAGCCTCC TGCCTATCCT CTCTCTGCCC CAGATGAAGA GACCCCCCTT 
GGACCCTGCT GTTCGCCCCC CCAACCCCAG GACTACATGA GTGTTGAGGT GGGGGGTCTG 
GGAAAGAACC AGTCGCCCAG GCCTAGCCTT GTTGCCCCCT GGAACCCCCC CAGCTATATC 
AGACCTTTAG CTAGTAACCC TGGGGCGCAT GATGGGGGGC ACTGGAAGTC AGTGGGAGAA 
GATTACACTG ACATGACCTT TAACCTCAGT GGGGGTGGGA AAGCTCAGAG CACTACAGCT 
ATGCTGCAGC ATCTCTGCGT GATGGAGGGG CATTACAAAC ACACCTTCTC CTCACCCGTT 
TCTCCCCCCT GCCTGAACCG GCCACCCACG CAGCAGTCGG AGCCCAAAGT GGTACGAGCT 
GACCCCCAAG GCAGAAGAAG ACACAGTTCA GAGACGTTCT CTTCCAGCTC CACCCCTGCA 
GGAGAAGGGC TCAGCTCCTC CCCCCCCAAC TCCTCACAGG CTGACCCCAC AGCCCACTGT 
GAGTCTTACC TGGTGGGGGG ACAAGCTTCC AGATGGGCCA ACTCAGCATC CTTTGACTGT 
GTGTGGATGT CTGTGGAGGG GGTCAGAGAC TCGCCAGCTC CCCATGACTC ACCAGGAGCC 
ACAGAGGCCC CGCCCCCTTT CTCTGCCGCA GAGGCCGGAT CCGGCAGAAT GTGCAGGAAC 
GCGTCTGCCG GCTTCCAGAA CGGACTCAAC TACATCGCCT TGGAGCTAAG GGAGGAAGGG 
GTGAACACGG AGGCCGTGGC GTCTGGAGCG ACCGGCACCA ACGGGGACAC AGCTGGGGAG 
GGAACGGTGC CTCCGCCTGA GAACGGAGCC TATGCCAGTA TAGACTTCAC CAAAACGGAC 
GGAGTCGCAG CTGCCAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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LOC101169068 cDNA ORF clone, Oryzias latipes(Japanese medaka)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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