LOC101166347 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101166347
Entrez Gene ID	101166347
Full Name	trichohyalin-like
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023950728.1	XP_023806496.1	trichohyalin isoform X1
XM_023950729.1	XP_023806497.1	trichohyalin isoform X1
XM_023950730.1	XP_023806498.1	trichohyalin isoform X2
XM_023950731.1	XP_023806499.1	trichohyalin isoform X3
XM_020713302.1	XP_020568961.1	trichohyalin-like

Pathways from BioSystems
Gene Ontology

The following LOC101166347 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101166347 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa113158	XM_023950729.1 Latest version!	Oryzias latipes trichohyalin (LOC101166347), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OJa113158	XM_023950728.1 Latest version!	Oryzias latipes trichohyalin (LOC101166347), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OJa113159	XM_023950730.1 Latest version!	Oryzias latipes trichohyalin (LOC101166347), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OJa113160	XM_023950731.1 Latest version!	Oryzias latipes trichohyalin (LOC101166347), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OJa75542	XM_020713302.1 Latest version!	Oryzias latipes trichohyalin-like (LOC101166347), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101166347 ( XM_023950728.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806496.1 Oryzias latipes trichohyalin (LOC101166347), transcript variant X1, mRNA.

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CloneID	OJa113158
Clone ID Related Accession (Same CDS sequence)	XM_023950728.1 , XM_023950729.1
Accession Version	XM_023950728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	trichohyalin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG GGTTGCTGAT TGGCTGGCAG CAGCAGAAGG AGGAGCTCGA GCGGGAGGTA 
TGCCGTCTGC AGGAGGAGCT GGCAGAGAGT CGAGCGGAGC GGGAGGAGTT GGAGTCCAGA 
ACCCGAGCTC TGCAGGACAG GGTGAGGCTC CGTCAGACCA TACATCCGTC ACTTGGCCTG 
TCACTTGAGG AGGAGGAGGA GCAGAGGAGG TGGAAGAAGC AGCTTAGGGA GGGCAGGGAG 
AGAGAGGCCA GGCAGGCACT GCTGATACAT CGGCTGCAGA ACAAGGTTCT GGAGTACAGA 
GATCAGTGCC AGCATTTGGA CCTGCAGTTG CAAGATGAAC GGAGGAAGAC GCTCCACATG 
GAGGGGGTCC GGGATGAACA TAGCAACTCT CTGGAAAGCG CCCTCATCAG GCTGGAGGAG 
GAGCAGCACA GGTCCGTAAG TCTGGCTGAC ACCAACTCTC TTCTGCGGGA GCAGCTCAGC 
CAATCTGAGA AGTTCAACCA GGAGCTTAAG GAGGAACTCC ACAGACTGAC AGCTGATTGG 
ATCAGATCCA CGGAGAAGGC GGAGCAAAGA GAAACTGATT GGCAGAGAGA GAAAGAGGAT 
CAGCGGGGTC ACGTGGGTCA GCAGCAGAAC AACTGGCTGT CAGTTTGGAG ATCCGTGGTG 
GCTCTGAGGA GACAATGTCA CAGCGTGAAA ACAGCTGCCG ACAGAGACCT GTGGGAGCTC 
AGGGCCGAGT TTTCCCGTCT CTCCTCAACA CTCCTCTCCA GCTGTGACTC TGTCTGCTCC 
ACCCAGAGCC TGGCGGCTCT TCACAAGCCT TTGTCTTCAA CTTTGCTGCC TCTTCCTTCC 
CCACCATCCA GTTCTCCTCC TCTATCCTCA ACACTGATCC TCCCTCCTCC TCCTCCAGAC 
TCCTGCTCCA CTCAGGGATC CTTCTCCATG GGGGAGCTAG AACTCAAAGA GGAGGAGGGA 
GAAGGTTCTG AGCCTAAGCT CTATGAAAAG ACTGAAGCTT TGCAGCAGAG GACTGTGGAG 
CTTGCTCGCT CACTGCAGGA TGAGAGAGAG GAGAAGGAGA GGGAGGTGGA AAGACATCAA 
GAAACTGCAA GGAGACTCCA GTCTGTCAGT CAGGCTGTGA TCAGACTGGC CAGAGTCGTC 
AGCAGCAGAA GTCGGCCAGC GAGCACCTCC CCAGAAGACG TCCTGCGGGC GGATTTGTCC 
TCCATGCTGG CTGTGCTCTC ACAGGCGGAA AGCGTCCTGC TGAGGAGGAG CGAGGAGCTG 
CAGGGGGCGG AGCTAAACCT GCGACGTCTC GGTGAGGTGA GAACCAGCTT GCAGCTGCAG 
GTGAAGCAGC TTCAGGACGA CCACCAGCAG CTTCTGGCAC GCTCACAACA CACACAGCAG 
GAGCTCACGC ACACACAGAG CGTCCTCAGC AGCGAGAAAA AGGTCTCCAG CTCCCTCCGC 
TTGCAGCTGG AGGAGGTGCA AAGAAGAGAG GAGGAGGTGA AGAGAGAGAA CCACAGACTG 
AGGAGAGAGA GAGACAGAGA GGAAGAGAAG AATCTGCAAC TGGAAACAGA CTCACATCGA 
CGGGTGGAGG CGGAGCTCCT AGAAAGTGTC CAGCTGACGG AGAGAGAACA TCTGCACCAG 
ATGGAGCTCC TCAGTCTGAA GGGGGCGCTG GAGAGAGAAC AGCTGGACAG GCAGAAAGCA 
GAGGAAGAGG CAGCAGGCTC TAGGGATGCC CTACAGCAGT GCAGGGGAAG CATACTCCGC 
CTCTCATCCT CAGAAAGCCT GCTGAGGCGG GATCTGGAGG AGAGTCGAGA TGCTTTGAAG 
AAGGTTTCTG CTCTTAACTC TGCTCTGGCC TCAGACAAGA GAGCACTGAA CCAGCAGCTG 
CTGCAGCTGG AAGGCAAACT GTCAGAGAGC CAATCGCAGC TGCAAGCTGT GAGTTCAGAG 
TTCACCTGTC TGCAGAGAGA CGTCAGACCT CTCAGACTGC AGAGGGATCA GGATGCCGAC 
GTCATCCGTC AGCTGAAAGA GCAGCAGGTG GAGGCTGAGA AAAACCTGGA GAGAAAGGAG 
AGAGAGCTTC AGCTATTGGA GGAGGAGAGA ACAAGTGGTG AACAACAGCT GGAGGAGCTT 
CGCTCTCATC ACGTCTCGCT GTGCCAGGAG CTGAAGGAGT TGCAGGAGGA GTTGCAACAG 
GCGAAACAGG AGGTTAGGAT TCTGAATTGG CAGCAGGAGG AGCAGCAAAG GGAGAGCAGG 
AGCCGGCAGG AGGAGCTCCA GAATGAGCAT AGACAGCGGG CGCAGCTGGA GGAGGAGCTG 
CATGAGCTCA GGCCTCTGTC AGTGTCCCTG CAGCTGCAGC TCAGCCAGCA GCAGCAGCTC 
CTCTCCCAGG TCGAGGTGGA CCGCTGTCAG CTGAGCACTC GCCTCCATGC GCTGCAGGAG 
GCCAAGCTCA CCTTACAGGG TGAAATCAAA TGTCTGAGAG GGGAGCTGCA GAGAGAGACA 
GCCAGCAGAG AGAGAAGTGA GGGCGAGAAG GAGGTGCTGC AGGAAGACCT GCAGAGACTG 
ACGGAAGAGG TGCAGGAGCT GAGGAGAAGA AAGGCAGAAA AGGAAGAGGA GAAAAAGATG 
TGCCAAAGAG AACGAGAGAC ACTGAGGGAG GAATTAAGGC TGAGAGACGG GGAGCTGGAA 
GCCCTGAAGA CACGCACTGA AAGGATGGAA GAGGAGCTGG AAGAAAAGGG ACAACAGGTG 
GAGAGACTGA AGGAGGAGGT GACACAACGA GCTACCCAAG TCAGCTTCCT AAATGAGAGA 
ATAGTTGGCT TGGAAGAGGA AAAGAAGATT CTGCTGGAGG AGGCAAAGAG TACTGAGGTC 
AGACTGCTGG AGGAGGAGGC GAAAAGGAAA GACGAGGAGG TGCAAAGAGA TGGGGAGACT 
GAAGCTCTCT GTGACAGAAT AGAGAAATTA GAAAGAGAGA AGGAAGAAAT AGATAAGGAG 
GTGCACCAAA TGAAGAGAAA AGCACAGGAG CAAGAGGAGA GATGGAGGTC AAATGATGAG 
GAGAGCCAGA ATGAGATAAA CAGACTAAGG GGAGAAAACT CTTCCCTCCA GGAGCACAGA 
AAGTCAGAGA AGCGACAAAT AGAGAAACTG CAAAATGAAA AAATGGAGGT GCTGGAGAGG 
CTACAAGAGA AAGTTGGAGA GGTGGAACTG ATGAAGGTAA AACTAAGTGT GGCTCAGGAG 
GAGAGTGAGG AGCTGAGGGA GGAGGTGCAG AGGAGGGAGC GCAGCCTGGA GAAACAGATA 
TGTGCAGTAC GAGAAAGGGA GGAGGAAATG GAGAACCTGA GGGGGCGGCT GAGGTTGGTG 
AAGGAGCGGG AGGCGGAAGC AGAGAGAGAG CTCCAGGAAA CTTCTGACAA ACTGAAAAAA 
AAAGAGGCGA GGGAGGAACA CCTGGAGGTG CTGCTTAGAG AAAACCGGGA ACTTTTGAAG 
CAGGAGGAAA AAGGACGAGA AGAAAGAGAA GACAGGATCT GCTCCTTGAT CAAACAATTG 
CAGGAGGCCC AGGCTGTTAA CCTGGGAGCA AAAAGAAGAA GCACAGAAAG GCAGAAGGCC 
CATGACAGAC TGCAGGAGGA GGTGAAGGAC TGGCAGGAGC AAGCAGAACA AAGTGTGAAG 
GAGGTGGCAA AACTCAAAGA AATCCTGAAG GGGAGAAATG AGGAGGTGAA CCAGCTGAAA 
GCAATGCTTG AAGAAAAAGA AGAGGAGTGG AAAGAGCAGG AGGCTTTGAG TAAAACAGAG 
AAAGAAAGGA GGAGAAGGCT GGAAAACAAG CTGGTCGAGG TGGAGGAAGA GAACACCAGG 
TTAGAGAAGG AGGTGAGACT GATGCAGGCA GTTGGAGATA AAGATAGGGA GGCTCTGGCA 
AAGGCTGGAG AGGAGTTAAA AACATTGAAG GACAACCTGG TAAAAAGTGA AAACAAAGAG 
AGGGAGCTGG GAGAAACTGT GAGAAGAATG GAAGAGGAGA AGATGATGCT GAGTAGCAAT 
TTGAGAGAGA TGGAGGACGA GATCAAAGCA GAGAAGGAGC AGTTGTGGAG AGTAAGGAAA 
GAGAGGAGCA GTCTGGAGGA CAAACTAAAA GAAAAAGAGA GAGAAATAGA TGAGTGCAGG 
GATTTGCTTA GAAGTCAGGA GGAAAAACTT GAGGAGGTGA AAAAGATGGA TAGAAAAACA 
TTGGAGAGTG AAAGAAATCG GTATCTACTG TTGGAGGAAA AAGGGAAGAC CAGGGTTAAA 
AAGCTTGAGG AGGAGAAGGA GGACCTTTTA CTGCAGTTAA TGAAAAGGGA AAAAGAAGTG 
ACAGAACTCC AAGAGGAGGT GCAGAGGCAG AGGATCACTG CAAGCAAGGA GCTCAATGAA 
AAGAAAGAGG AGGCATTTAA TGTACAAAGG GAATGTGAGG AGGCGCAGGA AAAGCTAAAA 
TCATTGAAAA GGGAAATGAT TTATCTCCAA GAGGAGGTGA AACAAAAGCA AGAAGAGAAT 
GAGGAGGTGC AGGAAAAACT GCAATCATTG AAGAGGGAAA TGATTTATCT CCAAGAGGAG 
GTGAAACAAA AGCAAGAAGA GAATGAGGAG GTGCAGGAAA AACTGCAATC ATTGAAGAGG 
GAAATGATTT ATCTCCAGGA GGAGGTGAAA CAAAAGCAAG AAGACAATGA GGAGGTGCAG 
GAAAAACTCC AATCATTAAA AAGCGAAATG ATTTATCTCC AAGAGGAGGT GAAACAAAAG 
CAAAAAGAGA ATGAGGAGAC ACAGGAGAAA ATGACCGTGT TAAAGGAGGT GCACCAGAAA 
CATTTAAAGA AAGAGGAAGA GTGGAGAACT AACGATATGA TGAGCAGAAA TGACACCCAG 
GAGCTACAGA GGCAGAAAGA TGAGGCTCAA GAGGAGCTGC AGAGGAGCAG AAAGGAGATC 
TACATACTTA GAGAGGAGCT AGAAAAGGAA CAAAGAGAAA AAAAGGACCT TCAAGATGAG 
ATGAAAAAGA GGAGTGAAGA GGCTGGATTG CTAAGACAGA AAATAGAGGA GGAGTTCAGC 
AGCACCAAGA AGAGGGAGGA GACAGAAAAA CAAACAAGAG AGAAGACTGA GATAGAGTTG 
GCCGCTCTAA AGGAGGAGCT GCAAAGGGAA CACAGGGAGA AATTGGAGAC AGTGGAGGAG 
CTGAAGAAGA GGAGAGAGGA TGTCACTGTG CTTGAAGGAG AGGTGAAGGA GGAGTGCATG 
AAGAAGGAGG AGGTGCAGGA AGATCTAAGA AGAGTGCAGC ACAGCTTGGA GCAGATGTCT 
CACAGTCTGA GCTCCCTTCA GAGTCAGGTG TCAGAGCTGA GTATCAGCAA GGAGCGAGTA 
GAGGTGGAGA AGCAGCAGAT GAGAGAAGGC CTGCAGGTGG CGCTCAAGGA GATGGACTTG 
CTGAAAGTTC TCCTCCAGGA AAGCCATGCA GAAGGAGAAC TGCTGAGGAG CACACTGGAG 
GAGAAGAAGA AGGAGATGGG GAGGGTTCGA CAGGAGAACC TGCAGATGGA AGAGAGGCAA 
GAGGAGCTGG AGGAGCTGAG AGCCAGGGTC AAAGCCCTGG AGAGAAGAAG GACGGAGATG 
GCAGAGGAGC TAGATAGGGC AGATGAGAGG AGGAGGGAGG CTGAGGAGTG GTGGAGGAGC 
AAAGTGAAGG AAGTAGAACG GCAGAAGGGG ACGATGCAAA GCACTCTTTA CACAGAAATA 
CAGACGCAAG GGGAGCTACT GAAGGAGTCG AGGTCCAAGC TTCAGGACAG TTTGAGGGAG 
CTGGAGGAGG CGAGAATGGA GCTTAGCCGG AACAGAGCCT TGTTGGAGGA GCAGAAAACT 
TTCACGATTG CTGACAGAGA GGATTCGGGG GAGCAGAGGA GCAGAGGCAG CCAAGAGGAA 
AGAGGGGAGG TGAAGCAGGA GGAGATCAGG AGGCAGCTCC AGCAGAGGGA GGCTCAGGTG 
GACAGTTTGA CTCTCAGAGT CCAGGAGCTG CAGGAAGACA GAAATCAGCT CCGATTGGCT 
CTGGAACAAA CTGAGGCGCA GCTGAAAAAC GTCGCCACCC AGGTGGAGCA GAGCACGGGG 
GCGGAGCCTA ACCCAGGAGA GACTGTAGGG GGCAGACTGG AGGTCCTGCA GAGACTGGTG 
GCTGAACTGG AGCTCCAACG GACACGACTG CAGAAGAAGA ACTTGAATCT AGAAAAGCAA 
AAAGACAGAC TGAAGAAAGA CAGAGACTTT CTGAGAGACA CCCTGAGACA GGTGGAGCTG 
GAGCGTGGGA GGCTCAGCCA GCAGCTGACA GACAGCAGAG GATCTCAGGT GGAACAGGTG 
TGCGCTAATG CCACAGAAGA AGATCGTCTG CGCAGCAAAG TGAAGGAGCT GGAGGAGCAG 
GTTAGTCAGC TTCGTCTTTC TTTAGCTATG GACCAGCAGC AGAAGGCGGA GTTTATCCAG 
CAGTCATCCA GAAACAGCCA ATGGCTGCTC TCACTAAGAC GGGACCTCAA TGATTCGCTG 
TCTGCTGTGA CCCGTCTACC AATCCCGTCT GTCCTGGAGT CTGAGACACA GAGGTTGGAC 
CGCAGCCTGA GGGAGGAGGA GCTTAGGATT TCCTTGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806496.1
CDS	297..6818
Translation	MDSGLLIGWQQQKEELEREVCRLQEELAESRAEREELESRTRALQDRVRLRQTIHPSLGLSLEEEEEQRRWKKQLREGREREARQALLIHRLQNKVLEYRDQCQHLDLQLQDERRKTLHMEGVRDEHSNSLESALIRLEEEQHRSVSLADTNSLLREQLSQSEKFNQELKEELHRLTADWIRSTEKAEQRETDWQREKEDQRGHVGQQQNNWLSVWRSVVALRRQCHSVKTAADRDLWELRAEFSRLSSTLLSSCDSVCSTQSLAALHKPLSSTLLPLPSPPSSSPPLSSTLILPPPPPDSCSTQGSFSMGELELKEEEGEGSEPKLYEKTEALQQRTVELARSLQDEREEKEREVERHQETARRLQSVSQAVIRLARVVSSRSRPASTSPEDVLRADLSSMLAVLSQAESVLLRRSEELQGAELNLRRLGEVRTSLQLQVKQLQDDHQQLLARSQHTQQELTHTQSVLSSEKKVSSSLRLQLEEVQRREEEVKRENHRLRRERDREEEKNLQLETDSHRRVEAELLESVQLTEREHLHQMELLSLKGALEREQLDRQKAEEEAAGSRDALQQCRGSILRLSSSESLLRRDLEESRDALKKVSALNSALASDKRALNQQLLQLEGKLSESQSQLQAVSSEFTCLQRDVRPLRLQRDQDADVIRQLKEQQVEAEKNLERKERELQLLEEERTSGEQQLEELRSHHVSLCQELKELQEELQQAKQEVRILNWQQEEQQRESRSRQEELQNEHRQRAQLEEELHELRPLSVSLQLQLSQQQQLLSQVEVDRCQLSTRLHALQEAKLTLQGEIKCLRGELQRETASRERSEGEKEVLQEDLQRLTEEVQELRRRKAEKEEEKKMCQRERETLREELRLRDGELEALKTRTERMEEELEEKGQQVERLKEEVTQRATQVSFLNERIVGLEEEKKILLEEAKSTEVRLLEEEAKRKDEEVQRDGETEALCDRIEKLEREKEEIDKEVHQMKRKAQEQEERWRSNDEESQNEINRLRGENSSLQEHRKSEKRQIEKLQNEKMEVLERLQEKVGEVELMKVKLSVAQEESEELREEVQRRERSLEKQICAVREREEEMENLRGRLRLVKEREAEAERELQETSDKLKKKEAREEHLEVLLRENRELLKQEEKGREEREDRICSLIKQLQEAQAVNLGAKRRSTERQKAHDRLQEEVKDWQEQAEQSVKEVAKLKEILKGRNEEVNQLKAMLEEKEEEWKEQEALSKTEKERRRRLENKLVEVEEENTRLEKEVRLMQAVGDKDREALAKAGEELKTLKDNLVKSENKERELGETVRRMEEEKMMLSSNLREMEDEIKAEKEQLWRVRKERSSLEDKLKEKEREIDECRDLLRSQEEKLEEVKKMDRKTLESERNRYLLLEEKGKTRVKKLEEEKEDLLLQLMKREKEVTELQEEVQRQRITASKELNEKKEEAFNVQRECEEAQEKLKSLKREMIYLQEEVKQKQEENEEVQEKLQSLKREMIYLQEEVKQKQEENEEVQEKLQSLKREMIYLQEEVKQKQEDNEEVQEKLQSLKSEMIYLQEEVKQKQKENEETQEKMTVLKEVHQKHLKKEEEWRTNDMMSRNDTQELQRQKDEAQEELQRSRKEIYILREELEKEQREKKDLQDEMKKRSEEAGLLRQKIEEEFSSTKKREETEKQTREKTEIELAALKEELQREHREKLETVEELKKRREDVTVLEGEVKEECMKKEEVQEDLRRVQHSLEQMSHSLSSLQSQVSELSISKERVEVEKQQMREGLQVALKEMDLLKVLLQESHAEGELLRSTLEEKKKEMGRVRQENLQMEERQEELEELRARVKALERRRTEMAEELDRADERRREAEEWWRSKVKEVERQKGTMQSTLYTEIQTQGELLKESRSKLQDSLRELEEARMELSRNRALLEEQKTFTIADREDSGEQRSRGSQEERGEVKQEEIRRQLQQREAQVDSLTLRVQELQEDRNQLRLALEQTEAQLKNVATQVEQSTGAEPNPGETVGGRLEVLQRLVAELELQRTRLQKKNLNLEKQKDRLKKDRDFLRDTLRQVELERGRLSQQLTDSRGSQVEQVCANATEEDRLRSKVKELEEQVSQLRLSLAMDQQQKAEFIQQSSRNSQWLLSLRRDLNDSLSAVTRLPIPSVLESETQRLDRSLREEELRISLS

Target ORF information:

RefSeq Version	XM_023950728.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes trichohyalin (LOC101166347), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950728.1

ORF Insert Sequence:

ATGGATTCTG GGTTGCTGAT TGGCTGGCAG CAGCAGAAGG AGGAGCTCGA GCGGGAGGTA 
TGCCGTCTGC AGGAGGAGCT GGCAGAGAGT CGAGCGGAGC GGGAGGAGTT GGAGTCCAGA 
ACCCGAGCTC TGCAGGACAG GGTGAGGCTC CGTCAGACCA TACATCCGTC ACTTGGCCTG 
TCACTTGAGG AGGAGGAGGA GCAGAGGAGG TGGAAGAAGC AGCTTAGGGA GGGCAGGGAG 
AGAGAGGCCA GGCAGGCACT GCTGATACAT CGGCTGCAGA ACAAGGTTCT GGAGTACAGA 
GATCAGTGCC AGCATTTGGA CCTGCAGTTG CAAGATGAAC GGAGGAAGAC GCTCCACATG 
GAGGGGGTCC GGGATGAACA TAGCAACTCT CTGGAAAGCG CCCTCATCAG GCTGGAGGAG 
GAGCAGCACA GGTCCGTAAG TCTGGCTGAC ACCAACTCTC TTCTGCGGGA GCAGCTCAGC 
CAATCTGAGA AGTTCAACCA GGAGCTTAAG GAGGAACTCC ACAGACTGAC AGCTGATTGG 
ATCAGATCCA CGGAGAAGGC GGAGCAAAGA GAAACTGATT GGCAGAGAGA GAAAGAGGAT 
CAGCGGGGTC ACGTGGGTCA GCAGCAGAAC AACTGGCTGT CAGTTTGGAG ATCCGTGGTG 
GCTCTGAGGA GACAATGTCA CAGCGTGAAA ACAGCTGCCG ACAGAGACCT GTGGGAGCTC 
AGGGCCGAGT TTTCCCGTCT CTCCTCAACA CTCCTCTCCA GCTGTGACTC TGTCTGCTCC 
ACCCAGAGCC TGGCGGCTCT TCACAAGCCT TTGTCTTCAA CTTTGCTGCC TCTTCCTTCC 
CCACCATCCA GTTCTCCTCC TCTATCCTCA ACACTGATCC TCCCTCCTCC TCCTCCAGAC 
TCCTGCTCCA CTCAGGGATC CTTCTCCATG GGGGAGCTAG AACTCAAAGA GGAGGAGGGA 
GAAGGTTCTG AGCCTAAGCT CTATGAAAAG ACTGAAGCTT TGCAGCAGAG GACTGTGGAG 
CTTGCTCGCT CACTGCAGGA TGAGAGAGAG GAGAAGGAGA GGGAGGTGGA AAGACATCAA 
GAAACTGCAA GGAGACTCCA GTCTGTCAGT CAGGCTGTGA TCAGACTGGC CAGAGTCGTC 
AGCAGCAGAA GTCGGCCAGC GAGCACCTCC CCAGAAGACG TCCTGCGGGC GGATTTGTCC 
TCCATGCTGG CTGTGCTCTC ACAGGCGGAA AGCGTCCTGC TGAGGAGGAG CGAGGAGCTG 
CAGGGGGCGG AGCTAAACCT GCGACGTCTC GGTGAGGTGA GAACCAGCTT GCAGCTGCAG 
GTGAAGCAGC TTCAGGACGA CCACCAGCAG CTTCTGGCAC GCTCACAACA CACACAGCAG 
GAGCTCACGC ACACACAGAG CGTCCTCAGC AGCGAGAAAA AGGTCTCCAG CTCCCTCCGC 
TTGCAGCTGG AGGAGGTGCA AAGAAGAGAG GAGGAGGTGA AGAGAGAGAA CCACAGACTG 
AGGAGAGAGA GAGACAGAGA GGAAGAGAAG AATCTGCAAC TGGAAACAGA CTCACATCGA 
CGGGTGGAGG CGGAGCTCCT AGAAAGTGTC CAGCTGACGG AGAGAGAACA TCTGCACCAG 
ATGGAGCTCC TCAGTCTGAA GGGGGCGCTG GAGAGAGAAC AGCTGGACAG GCAGAAAGCA 
GAGGAAGAGG CAGCAGGCTC TAGGGATGCC CTACAGCAGT GCAGGGGAAG CATACTCCGC 
CTCTCATCCT CAGAAAGCCT GCTGAGGCGG GATCTGGAGG AGAGTCGAGA TGCTTTGAAG 
AAGGTTTCTG CTCTTAACTC TGCTCTGGCC TCAGACAAGA GAGCACTGAA CCAGCAGCTG 
CTGCAGCTGG AAGGCAAACT GTCAGAGAGC CAATCGCAGC TGCAAGCTGT GAGTTCAGAG 
TTCACCTGTC TGCAGAGAGA CGTCAGACCT CTCAGACTGC AGAGGGATCA GGATGCCGAC 
GTCATCCGTC AGCTGAAAGA GCAGCAGGTG GAGGCTGAGA AAAACCTGGA GAGAAAGGAG 
AGAGAGCTTC AGCTATTGGA GGAGGAGAGA ACAAGTGGTG AACAACAGCT GGAGGAGCTT 
CGCTCTCATC ACGTCTCGCT GTGCCAGGAG CTGAAGGAGT TGCAGGAGGA GTTGCAACAG 
GCGAAACAGG AGGTTAGGAT TCTGAATTGG CAGCAGGAGG AGCAGCAAAG GGAGAGCAGG 
AGCCGGCAGG AGGAGCTCCA GAATGAGCAT AGACAGCGGG CGCAGCTGGA GGAGGAGCTG 
CATGAGCTCA GGCCTCTGTC AGTGTCCCTG CAGCTGCAGC TCAGCCAGCA GCAGCAGCTC 
CTCTCCCAGG TCGAGGTGGA CCGCTGTCAG CTGAGCACTC GCCTCCATGC GCTGCAGGAG 
GCCAAGCTCA CCTTACAGGG TGAAATCAAA TGTCTGAGAG GGGAGCTGCA GAGAGAGACA 
GCCAGCAGAG AGAGAAGTGA GGGCGAGAAG GAGGTGCTGC AGGAAGACCT GCAGAGACTG 
ACGGAAGAGG TGCAGGAGCT GAGGAGAAGA AAGGCAGAAA AGGAAGAGGA GAAAAAGATG 
TGCCAAAGAG AACGAGAGAC ACTGAGGGAG GAATTAAGGC TGAGAGACGG GGAGCTGGAA 
GCCCTGAAGA CACGCACTGA AAGGATGGAA GAGGAGCTGG AAGAAAAGGG ACAACAGGTG 
GAGAGACTGA AGGAGGAGGT GACACAACGA GCTACCCAAG TCAGCTTCCT AAATGAGAGA 
ATAGTTGGCT TGGAAGAGGA AAAGAAGATT CTGCTGGAGG AGGCAAAGAG TACTGAGGTC 
AGACTGCTGG AGGAGGAGGC GAAAAGGAAA GACGAGGAGG TGCAAAGAGA TGGGGAGACT 
GAAGCTCTCT GTGACAGAAT AGAGAAATTA GAAAGAGAGA AGGAAGAAAT AGATAAGGAG 
GTGCACCAAA TGAAGAGAAA AGCACAGGAG CAAGAGGAGA GATGGAGGTC AAATGATGAG 
GAGAGCCAGA ATGAGATAAA CAGACTAAGG GGAGAAAACT CTTCCCTCCA GGAGCACAGA 
AAGTCAGAGA AGCGACAAAT AGAGAAACTG CAAAATGAAA AAATGGAGGT GCTGGAGAGG 
CTACAAGAGA AAGTTGGAGA GGTGGAACTG ATGAAGGTAA AACTAAGTGT GGCTCAGGAG 
GAGAGTGAGG AGCTGAGGGA GGAGGTGCAG AGGAGGGAGC GCAGCCTGGA GAAACAGATA 
TGTGCAGTAC GAGAAAGGGA GGAGGAAATG GAGAACCTGA GGGGGCGGCT GAGGTTGGTG 
AAGGAGCGGG AGGCGGAAGC AGAGAGAGAG CTCCAGGAAA CTTCTGACAA ACTGAAAAAA 
AAAGAGGCGA GGGAGGAACA CCTGGAGGTG CTGCTTAGAG AAAACCGGGA ACTTTTGAAG 
CAGGAGGAAA AAGGACGAGA AGAAAGAGAA GACAGGATCT GCTCCTTGAT CAAACAATTG 
CAGGAGGCCC AGGCTGTTAA CCTGGGAGCA AAAAGAAGAA GCACAGAAAG GCAGAAGGCC 
CATGACAGAC TGCAGGAGGA GGTGAAGGAC TGGCAGGAGC AAGCAGAACA AAGTGTGAAG 
GAGGTGGCAA AACTCAAAGA AATCCTGAAG GGGAGAAATG AGGAGGTGAA CCAGCTGAAA 
GCAATGCTTG AAGAAAAAGA AGAGGAGTGG AAAGAGCAGG AGGCTTTGAG TAAAACAGAG 
AAAGAAAGGA GGAGAAGGCT GGAAAACAAG CTGGTCGAGG TGGAGGAAGA GAACACCAGG 
TTAGAGAAGG AGGTGAGACT GATGCAGGCA GTTGGAGATA AAGATAGGGA GGCTCTGGCA 
AAGGCTGGAG AGGAGTTAAA AACATTGAAG GACAACCTGG TAAAAAGTGA AAACAAAGAG 
AGGGAGCTGG GAGAAACTGT GAGAAGAATG GAAGAGGAGA AGATGATGCT GAGTAGCAAT 
TTGAGAGAGA TGGAGGACGA GATCAAAGCA GAGAAGGAGC AGTTGTGGAG AGTAAGGAAA 
GAGAGGAGCA GTCTGGAGGA CAAACTAAAA GAAAAAGAGA GAGAAATAGA TGAGTGCAGG 
GATTTGCTTA GAAGTCAGGA GGAAAAACTT GAGGAGGTGA AAAAGATGGA TAGAAAAACA 
TTGGAGAGTG AAAGAAATCG GTATCTACTG TTGGAGGAAA AAGGGAAGAC CAGGGTTAAA 
AAGCTTGAGG AGGAGAAGGA GGACCTTTTA CTGCAGTTAA TGAAAAGGGA AAAAGAAGTG 
ACAGAACTCC AAGAGGAGGT GCAGAGGCAG AGGATCACTG CAAGCAAGGA GCTCAATGAA 
AAGAAAGAGG AGGCATTTAA TGTACAAAGG GAATGTGAGG AGGCGCAGGA AAAGCTAAAA 
TCATTGAAAA GGGAAATGAT TTATCTCCAA GAGGAGGTGA AACAAAAGCA AGAAGAGAAT 
GAGGAGGTGC AGGAAAAACT GCAATCATTG AAGAGGGAAA TGATTTATCT CCAAGAGGAG 
GTGAAACAAA AGCAAGAAGA GAATGAGGAG GTGCAGGAAA AACTGCAATC ATTGAAGAGG 
GAAATGATTT ATCTCCAGGA GGAGGTGAAA CAAAAGCAAG AAGACAATGA GGAGGTGCAG 
GAAAAACTCC AATCATTAAA AAGCGAAATG ATTTATCTCC AAGAGGAGGT GAAACAAAAG 
CAAAAAGAGA ATGAGGAGAC ACAGGAGAAA ATGACCGTGT TAAAGGAGGT GCACCAGAAA 
CATTTAAAGA AAGAGGAAGA GTGGAGAACT AACGATATGA TGAGCAGAAA TGACACCCAG 
GAGCTACAGA GGCAGAAAGA TGAGGCTCAA GAGGAGCTGC AGAGGAGCAG AAAGGAGATC 
TACATACTTA GAGAGGAGCT AGAAAAGGAA CAAAGAGAAA AAAAGGACCT TCAAGATGAG 
ATGAAAAAGA GGAGTGAAGA GGCTGGATTG CTAAGACAGA AAATAGAGGA GGAGTTCAGC 
AGCACCAAGA AGAGGGAGGA GACAGAAAAA CAAACAAGAG AGAAGACTGA GATAGAGTTG 
GCCGCTCTAA AGGAGGAGCT GCAAAGGGAA CACAGGGAGA AATTGGAGAC AGTGGAGGAG 
CTGAAGAAGA GGAGAGAGGA TGTCACTGTG CTTGAAGGAG AGGTGAAGGA GGAGTGCATG 
AAGAAGGAGG AGGTGCAGGA AGATCTAAGA AGAGTGCAGC ACAGCTTGGA GCAGATGTCT 
CACAGTCTGA GCTCCCTTCA GAGTCAGGTG TCAGAGCTGA GTATCAGCAA GGAGCGAGTA 
GAGGTGGAGA AGCAGCAGAT GAGAGAAGGC CTGCAGGTGG CGCTCAAGGA GATGGACTTG 
CTGAAAGTTC TCCTCCAGGA AAGCCATGCA GAAGGAGAAC TGCTGAGGAG CACACTGGAG 
GAGAAGAAGA AGGAGATGGG GAGGGTTCGA CAGGAGAACC TGCAGATGGA AGAGAGGCAA 
GAGGAGCTGG AGGAGCTGAG AGCCAGGGTC AAAGCCCTGG AGAGAAGAAG GACGGAGATG 
GCAGAGGAGC TAGATAGGGC AGATGAGAGG AGGAGGGAGG CTGAGGAGTG GTGGAGGAGC 
AAAGTGAAGG AAGTAGAACG GCAGAAGGGG ACGATGCAAA GCACTCTTTA CACAGAAATA 
CAGACGCAAG GGGAGCTACT GAAGGAGTCG AGGTCCAAGC TTCAGGACAG TTTGAGGGAG 
CTGGAGGAGG CGAGAATGGA GCTTAGCCGG AACAGAGCCT TGTTGGAGGA GCAGAAAACT 
TTCACGATTG CTGACAGAGA GGATTCGGGG GAGCAGAGGA GCAGAGGCAG CCAAGAGGAA 
AGAGGGGAGG TGAAGCAGGA GGAGATCAGG AGGCAGCTCC AGCAGAGGGA GGCTCAGGTG 
GACAGTTTGA CTCTCAGAGT CCAGGAGCTG CAGGAAGACA GAAATCAGCT CCGATTGGCT 
CTGGAACAAA CTGAGGCGCA GCTGAAAAAC GTCGCCACCC AGGTGGAGCA GAGCACGGGG 
GCGGAGCCTA ACCCAGGAGA GACTGTAGGG GGCAGACTGG AGGTCCTGCA GAGACTGGTG 
GCTGAACTGG AGCTCCAACG GACACGACTG CAGAAGAAGA ACTTGAATCT AGAAAAGCAA 
AAAGACAGAC TGAAGAAAGA CAGAGACTTT CTGAGAGACA CCCTGAGACA GGTGGAGCTG 
GAGCGTGGGA GGCTCAGCCA GCAGCTGACA GACAGCAGAG GATCTCAGGT GGAACAGGTG 
TGCGCTAATG CCACAGAAGA AGATCGTCTG CGCAGCAAAG TGAAGGAGCT GGAGGAGCAG 
GTTAGTCAGC TTCGTCTTTC TTTAGCTATG GACCAGCAGC AGAAGGCGGA GTTTATCCAG 
CAGTCATCCA GAAACAGCCA ATGGCTGCTC TCACTAAGAC GGGACCTCAA TGATTCGCTG 
TCTGCTGTGA CCCGTCTACC AATCCCGTCT GTCCTGGAGT CTGAGACACA GAGGTTGGAC 
CGCAGCCTGA GGGAGGAGGA GCTTAGGATT TCCTTGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101166347 ( XM_023950729.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806497.1 Oryzias latipes trichohyalin (LOC101166347), transcript variant X2, mRNA.

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CloneID	OJa113158
Clone ID Related Accession (Same CDS sequence)	XM_023950728.1 , XM_023950729.1
Accession Version	XM_023950729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	trichohyalin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG GGTTGCTGAT TGGCTGGCAG CAGCAGAAGG AGGAGCTCGA GCGGGAGGTA 
TGCCGTCTGC AGGAGGAGCT GGCAGAGAGT CGAGCGGAGC GGGAGGAGTT GGAGTCCAGA 
ACCCGAGCTC TGCAGGACAG GGTGAGGCTC CGTCAGACCA TACATCCGTC ACTTGGCCTG 
TCACTTGAGG AGGAGGAGGA GCAGAGGAGG TGGAAGAAGC AGCTTAGGGA GGGCAGGGAG 
AGAGAGGCCA GGCAGGCACT GCTGATACAT CGGCTGCAGA ACAAGGTTCT GGAGTACAGA 
GATCAGTGCC AGCATTTGGA CCTGCAGTTG CAAGATGAAC GGAGGAAGAC GCTCCACATG 
GAGGGGGTCC GGGATGAACA TAGCAACTCT CTGGAAAGCG CCCTCATCAG GCTGGAGGAG 
GAGCAGCACA GGTCCGTAAG TCTGGCTGAC ACCAACTCTC TTCTGCGGGA GCAGCTCAGC 
CAATCTGAGA AGTTCAACCA GGAGCTTAAG GAGGAACTCC ACAGACTGAC AGCTGATTGG 
ATCAGATCCA CGGAGAAGGC GGAGCAAAGA GAAACTGATT GGCAGAGAGA GAAAGAGGAT 
CAGCGGGGTC ACGTGGGTCA GCAGCAGAAC AACTGGCTGT CAGTTTGGAG ATCCGTGGTG 
GCTCTGAGGA GACAATGTCA CAGCGTGAAA ACAGCTGCCG ACAGAGACCT GTGGGAGCTC 
AGGGCCGAGT TTTCCCGTCT CTCCTCAACA CTCCTCTCCA GCTGTGACTC TGTCTGCTCC 
ACCCAGAGCC TGGCGGCTCT TCACAAGCCT TTGTCTTCAA CTTTGCTGCC TCTTCCTTCC 
CCACCATCCA GTTCTCCTCC TCTATCCTCA ACACTGATCC TCCCTCCTCC TCCTCCAGAC 
TCCTGCTCCA CTCAGGGATC CTTCTCCATG GGGGAGCTAG AACTCAAAGA GGAGGAGGGA 
GAAGGTTCTG AGCCTAAGCT CTATGAAAAG ACTGAAGCTT TGCAGCAGAG GACTGTGGAG 
CTTGCTCGCT CACTGCAGGA TGAGAGAGAG GAGAAGGAGA GGGAGGTGGA AAGACATCAA 
GAAACTGCAA GGAGACTCCA GTCTGTCAGT CAGGCTGTGA TCAGACTGGC CAGAGTCGTC 
AGCAGCAGAA GTCGGCCAGC GAGCACCTCC CCAGAAGACG TCCTGCGGGC GGATTTGTCC 
TCCATGCTGG CTGTGCTCTC ACAGGCGGAA AGCGTCCTGC TGAGGAGGAG CGAGGAGCTG 
CAGGGGGCGG AGCTAAACCT GCGACGTCTC GGTGAGGTGA GAACCAGCTT GCAGCTGCAG 
GTGAAGCAGC TTCAGGACGA CCACCAGCAG CTTCTGGCAC GCTCACAACA CACACAGCAG 
GAGCTCACGC ACACACAGAG CGTCCTCAGC AGCGAGAAAA AGGTCTCCAG CTCCCTCCGC 
TTGCAGCTGG AGGAGGTGCA AAGAAGAGAG GAGGAGGTGA AGAGAGAGAA CCACAGACTG 
AGGAGAGAGA GAGACAGAGA GGAAGAGAAG AATCTGCAAC TGGAAACAGA CTCACATCGA 
CGGGTGGAGG CGGAGCTCCT AGAAAGTGTC CAGCTGACGG AGAGAGAACA TCTGCACCAG 
ATGGAGCTCC TCAGTCTGAA GGGGGCGCTG GAGAGAGAAC AGCTGGACAG GCAGAAAGCA 
GAGGAAGAGG CAGCAGGCTC TAGGGATGCC CTACAGCAGT GCAGGGGAAG CATACTCCGC 
CTCTCATCCT CAGAAAGCCT GCTGAGGCGG GATCTGGAGG AGAGTCGAGA TGCTTTGAAG 
AAGGTTTCTG CTCTTAACTC TGCTCTGGCC TCAGACAAGA GAGCACTGAA CCAGCAGCTG 
CTGCAGCTGG AAGGCAAACT GTCAGAGAGC CAATCGCAGC TGCAAGCTGT GAGTTCAGAG 
TTCACCTGTC TGCAGAGAGA CGTCAGACCT CTCAGACTGC AGAGGGATCA GGATGCCGAC 
GTCATCCGTC AGCTGAAAGA GCAGCAGGTG GAGGCTGAGA AAAACCTGGA GAGAAAGGAG 
AGAGAGCTTC AGCTATTGGA GGAGGAGAGA ACAAGTGGTG AACAACAGCT GGAGGAGCTT 
CGCTCTCATC ACGTCTCGCT GTGCCAGGAG CTGAAGGAGT TGCAGGAGGA GTTGCAACAG 
GCGAAACAGG AGGTTAGGAT TCTGAATTGG CAGCAGGAGG AGCAGCAAAG GGAGAGCAGG 
AGCCGGCAGG AGGAGCTCCA GAATGAGCAT AGACAGCGGG CGCAGCTGGA GGAGGAGCTG 
CATGAGCTCA GGCCTCTGTC AGTGTCCCTG CAGCTGCAGC TCAGCCAGCA GCAGCAGCTC 
CTCTCCCAGG TCGAGGTGGA CCGCTGTCAG CTGAGCACTC GCCTCCATGC GCTGCAGGAG 
GCCAAGCTCA CCTTACAGGG TGAAATCAAA TGTCTGAGAG GGGAGCTGCA GAGAGAGACA 
GCCAGCAGAG AGAGAAGTGA GGGCGAGAAG GAGGTGCTGC AGGAAGACCT GCAGAGACTG 
ACGGAAGAGG TGCAGGAGCT GAGGAGAAGA AAGGCAGAAA AGGAAGAGGA GAAAAAGATG 
TGCCAAAGAG AACGAGAGAC ACTGAGGGAG GAATTAAGGC TGAGAGACGG GGAGCTGGAA 
GCCCTGAAGA CACGCACTGA AAGGATGGAA GAGGAGCTGG AAGAAAAGGG ACAACAGGTG 
GAGAGACTGA AGGAGGAGGT GACACAACGA GCTACCCAAG TCAGCTTCCT AAATGAGAGA 
ATAGTTGGCT TGGAAGAGGA AAAGAAGATT CTGCTGGAGG AGGCAAAGAG TACTGAGGTC 
AGACTGCTGG AGGAGGAGGC GAAAAGGAAA GACGAGGAGG TGCAAAGAGA TGGGGAGACT 
GAAGCTCTCT GTGACAGAAT AGAGAAATTA GAAAGAGAGA AGGAAGAAAT AGATAAGGAG 
GTGCACCAAA TGAAGAGAAA AGCACAGGAG CAAGAGGAGA GATGGAGGTC AAATGATGAG 
GAGAGCCAGA ATGAGATAAA CAGACTAAGG GGAGAAAACT CTTCCCTCCA GGAGCACAGA 
AAGTCAGAGA AGCGACAAAT AGAGAAACTG CAAAATGAAA AAATGGAGGT GCTGGAGAGG 
CTACAAGAGA AAGTTGGAGA GGTGGAACTG ATGAAGGTAA AACTAAGTGT GGCTCAGGAG 
GAGAGTGAGG AGCTGAGGGA GGAGGTGCAG AGGAGGGAGC GCAGCCTGGA GAAACAGATA 
TGTGCAGTAC GAGAAAGGGA GGAGGAAATG GAGAACCTGA GGGGGCGGCT GAGGTTGGTG 
AAGGAGCGGG AGGCGGAAGC AGAGAGAGAG CTCCAGGAAA CTTCTGACAA ACTGAAAAAA 
AAAGAGGCGA GGGAGGAACA CCTGGAGGTG CTGCTTAGAG AAAACCGGGA ACTTTTGAAG 
CAGGAGGAAA AAGGACGAGA AGAAAGAGAA GACAGGATCT GCTCCTTGAT CAAACAATTG 
CAGGAGGCCC AGGCTGTTAA CCTGGGAGCA AAAAGAAGAA GCACAGAAAG GCAGAAGGCC 
CATGACAGAC TGCAGGAGGA GGTGAAGGAC TGGCAGGAGC AAGCAGAACA AAGTGTGAAG 
GAGGTGGCAA AACTCAAAGA AATCCTGAAG GGGAGAAATG AGGAGGTGAA CCAGCTGAAA 
GCAATGCTTG AAGAAAAAGA AGAGGAGTGG AAAGAGCAGG AGGCTTTGAG TAAAACAGAG 
AAAGAAAGGA GGAGAAGGCT GGAAAACAAG CTGGTCGAGG TGGAGGAAGA GAACACCAGG 
TTAGAGAAGG AGGTGAGACT GATGCAGGCA GTTGGAGATA AAGATAGGGA GGCTCTGGCA 
AAGGCTGGAG AGGAGTTAAA AACATTGAAG GACAACCTGG TAAAAAGTGA AAACAAAGAG 
AGGGAGCTGG GAGAAACTGT GAGAAGAATG GAAGAGGAGA AGATGATGCT GAGTAGCAAT 
TTGAGAGAGA TGGAGGACGA GATCAAAGCA GAGAAGGAGC AGTTGTGGAG AGTAAGGAAA 
GAGAGGAGCA GTCTGGAGGA CAAACTAAAA GAAAAAGAGA GAGAAATAGA TGAGTGCAGG 
GATTTGCTTA GAAGTCAGGA GGAAAAACTT GAGGAGGTGA AAAAGATGGA TAGAAAAACA 
TTGGAGAGTG AAAGAAATCG GTATCTACTG TTGGAGGAAA AAGGGAAGAC CAGGGTTAAA 
AAGCTTGAGG AGGAGAAGGA GGACCTTTTA CTGCAGTTAA TGAAAAGGGA AAAAGAAGTG 
ACAGAACTCC AAGAGGAGGT GCAGAGGCAG AGGATCACTG CAAGCAAGGA GCTCAATGAA 
AAGAAAGAGG AGGCATTTAA TGTACAAAGG GAATGTGAGG AGGCGCAGGA AAAGCTAAAA 
TCATTGAAAA GGGAAATGAT TTATCTCCAA GAGGAGGTGA AACAAAAGCA AGAAGAGAAT 
GAGGAGGTGC AGGAAAAACT GCAATCATTG AAGAGGGAAA TGATTTATCT CCAAGAGGAG 
GTGAAACAAA AGCAAGAAGA GAATGAGGAG GTGCAGGAAA AACTGCAATC ATTGAAGAGG 
GAAATGATTT ATCTCCAGGA GGAGGTGAAA CAAAAGCAAG AAGACAATGA GGAGGTGCAG 
GAAAAACTCC AATCATTAAA AAGCGAAATG ATTTATCTCC AAGAGGAGGT GAAACAAAAG 
CAAAAAGAGA ATGAGGAGAC ACAGGAGAAA ATGACCGTGT TAAAGGAGGT GCACCAGAAA 
CATTTAAAGA AAGAGGAAGA GTGGAGAACT AACGATATGA TGAGCAGAAA TGACACCCAG 
GAGCTACAGA GGCAGAAAGA TGAGGCTCAA GAGGAGCTGC AGAGGAGCAG AAAGGAGATC 
TACATACTTA GAGAGGAGCT AGAAAAGGAA CAAAGAGAAA AAAAGGACCT TCAAGATGAG 
ATGAAAAAGA GGAGTGAAGA GGCTGGATTG CTAAGACAGA AAATAGAGGA GGAGTTCAGC 
AGCACCAAGA AGAGGGAGGA GACAGAAAAA CAAACAAGAG AGAAGACTGA GATAGAGTTG 
GCCGCTCTAA AGGAGGAGCT GCAAAGGGAA CACAGGGAGA AATTGGAGAC AGTGGAGGAG 
CTGAAGAAGA GGAGAGAGGA TGTCACTGTG CTTGAAGGAG AGGTGAAGGA GGAGTGCATG 
AAGAAGGAGG AGGTGCAGGA AGATCTAAGA AGAGTGCAGC ACAGCTTGGA GCAGATGTCT 
CACAGTCTGA GCTCCCTTCA GAGTCAGGTG TCAGAGCTGA GTATCAGCAA GGAGCGAGTA 
GAGGTGGAGA AGCAGCAGAT GAGAGAAGGC CTGCAGGTGG CGCTCAAGGA GATGGACTTG 
CTGAAAGTTC TCCTCCAGGA AAGCCATGCA GAAGGAGAAC TGCTGAGGAG CACACTGGAG 
GAGAAGAAGA AGGAGATGGG GAGGGTTCGA CAGGAGAACC TGCAGATGGA AGAGAGGCAA 
GAGGAGCTGG AGGAGCTGAG AGCCAGGGTC AAAGCCCTGG AGAGAAGAAG GACGGAGATG 
GCAGAGGAGC TAGATAGGGC AGATGAGAGG AGGAGGGAGG CTGAGGAGTG GTGGAGGAGC 
AAAGTGAAGG AAGTAGAACG GCAGAAGGGG ACGATGCAAA GCACTCTTTA CACAGAAATA 
CAGACGCAAG GGGAGCTACT GAAGGAGTCG AGGTCCAAGC TTCAGGACAG TTTGAGGGAG 
CTGGAGGAGG CGAGAATGGA GCTTAGCCGG AACAGAGCCT TGTTGGAGGA GCAGAAAACT 
TTCACGATTG CTGACAGAGA GGATTCGGGG GAGCAGAGGA GCAGAGGCAG CCAAGAGGAA 
AGAGGGGAGG TGAAGCAGGA GGAGATCAGG AGGCAGCTCC AGCAGAGGGA GGCTCAGGTG 
GACAGTTTGA CTCTCAGAGT CCAGGAGCTG CAGGAAGACA GAAATCAGCT CCGATTGGCT 
CTGGAACAAA CTGAGGCGCA GCTGAAAAAC GTCGCCACCC AGGTGGAGCA GAGCACGGGG 
GCGGAGCCTA ACCCAGGAGA GACTGTAGGG GGCAGACTGG AGGTCCTGCA GAGACTGGTG 
GCTGAACTGG AGCTCCAACG GACACGACTG CAGAAGAAGA ACTTGAATCT AGAAAAGCAA 
AAAGACAGAC TGAAGAAAGA CAGAGACTTT CTGAGAGACA CCCTGAGACA GGTGGAGCTG 
GAGCGTGGGA GGCTCAGCCA GCAGCTGACA GACAGCAGAG GATCTCAGGT GGAACAGGTG 
TGCGCTAATG CCACAGAAGA AGATCGTCTG CGCAGCAAAG TGAAGGAGCT GGAGGAGCAG 
GTTAGTCAGC TTCGTCTTTC TTTAGCTATG GACCAGCAGC AGAAGGCGGA GTTTATCCAG 
CAGTCATCCA GAAACAGCCA ATGGCTGCTC TCACTAAGAC GGGACCTCAA TGATTCGCTG 
TCTGCTGTGA CCCGTCTACC AATCCCGTCT GTCCTGGAGT CTGAGACACA GAGGTTGGAC 
CGCAGCCTGA GGGAGGAGGA GCTTAGGATT TCCTTGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806497.1
CDS	293..6814
Translation	MDSGLLIGWQQQKEELEREVCRLQEELAESRAEREELESRTRALQDRVRLRQTIHPSLGLSLEEEEEQRRWKKQLREGREREARQALLIHRLQNKVLEYRDQCQHLDLQLQDERRKTLHMEGVRDEHSNSLESALIRLEEEQHRSVSLADTNSLLREQLSQSEKFNQELKEELHRLTADWIRSTEKAEQRETDWQREKEDQRGHVGQQQNNWLSVWRSVVALRRQCHSVKTAADRDLWELRAEFSRLSSTLLSSCDSVCSTQSLAALHKPLSSTLLPLPSPPSSSPPLSSTLILPPPPPDSCSTQGSFSMGELELKEEEGEGSEPKLYEKTEALQQRTVELARSLQDEREEKEREVERHQETARRLQSVSQAVIRLARVVSSRSRPASTSPEDVLRADLSSMLAVLSQAESVLLRRSEELQGAELNLRRLGEVRTSLQLQVKQLQDDHQQLLARSQHTQQELTHTQSVLSSEKKVSSSLRLQLEEVQRREEEVKRENHRLRRERDREEEKNLQLETDSHRRVEAELLESVQLTEREHLHQMELLSLKGALEREQLDRQKAEEEAAGSRDALQQCRGSILRLSSSESLLRRDLEESRDALKKVSALNSALASDKRALNQQLLQLEGKLSESQSQLQAVSSEFTCLQRDVRPLRLQRDQDADVIRQLKEQQVEAEKNLERKERELQLLEEERTSGEQQLEELRSHHVSLCQELKELQEELQQAKQEVRILNWQQEEQQRESRSRQEELQNEHRQRAQLEEELHELRPLSVSLQLQLSQQQQLLSQVEVDRCQLSTRLHALQEAKLTLQGEIKCLRGELQRETASRERSEGEKEVLQEDLQRLTEEVQELRRRKAEKEEEKKMCQRERETLREELRLRDGELEALKTRTERMEEELEEKGQQVERLKEEVTQRATQVSFLNERIVGLEEEKKILLEEAKSTEVRLLEEEAKRKDEEVQRDGETEALCDRIEKLEREKEEIDKEVHQMKRKAQEQEERWRSNDEESQNEINRLRGENSSLQEHRKSEKRQIEKLQNEKMEVLERLQEKVGEVELMKVKLSVAQEESEELREEVQRRERSLEKQICAVREREEEMENLRGRLRLVKEREAEAERELQETSDKLKKKEAREEHLEVLLRENRELLKQEEKGREEREDRICSLIKQLQEAQAVNLGAKRRSTERQKAHDRLQEEVKDWQEQAEQSVKEVAKLKEILKGRNEEVNQLKAMLEEKEEEWKEQEALSKTEKERRRRLENKLVEVEEENTRLEKEVRLMQAVGDKDREALAKAGEELKTLKDNLVKSENKERELGETVRRMEEEKMMLSSNLREMEDEIKAEKEQLWRVRKERSSLEDKLKEKEREIDECRDLLRSQEEKLEEVKKMDRKTLESERNRYLLLEEKGKTRVKKLEEEKEDLLLQLMKREKEVTELQEEVQRQRITASKELNEKKEEAFNVQRECEEAQEKLKSLKREMIYLQEEVKQKQEENEEVQEKLQSLKREMIYLQEEVKQKQEENEEVQEKLQSLKREMIYLQEEVKQKQEDNEEVQEKLQSLKSEMIYLQEEVKQKQKENEETQEKMTVLKEVHQKHLKKEEEWRTNDMMSRNDTQELQRQKDEAQEELQRSRKEIYILREELEKEQREKKDLQDEMKKRSEEAGLLRQKIEEEFSSTKKREETEKQTREKTEIELAALKEELQREHREKLETVEELKKRREDVTVLEGEVKEECMKKEEVQEDLRRVQHSLEQMSHSLSSLQSQVSELSISKERVEVEKQQMREGLQVALKEMDLLKVLLQESHAEGELLRSTLEEKKKEMGRVRQENLQMEERQEELEELRARVKALERRRTEMAEELDRADERRREAEEWWRSKVKEVERQKGTMQSTLYTEIQTQGELLKESRSKLQDSLRELEEARMELSRNRALLEEQKTFTIADREDSGEQRSRGSQEERGEVKQEEIRRQLQQREAQVDSLTLRVQELQEDRNQLRLALEQTEAQLKNVATQVEQSTGAEPNPGETVGGRLEVLQRLVAELELQRTRLQKKNLNLEKQKDRLKKDRDFLRDTLRQVELERGRLSQQLTDSRGSQVEQVCANATEEDRLRSKVKELEEQVSQLRLSLAMDQQQKAEFIQQSSRNSQWLLSLRRDLNDSLSAVTRLPIPSVLESETQRLDRSLREEELRISLS

Target ORF information:

RefSeq Version	XM_023950729.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes trichohyalin (LOC101166347), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950729.1

ORF Insert Sequence:

ATGGATTCTG GGTTGCTGAT TGGCTGGCAG CAGCAGAAGG AGGAGCTCGA GCGGGAGGTA 
TGCCGTCTGC AGGAGGAGCT GGCAGAGAGT CGAGCGGAGC GGGAGGAGTT GGAGTCCAGA 
ACCCGAGCTC TGCAGGACAG GGTGAGGCTC CGTCAGACCA TACATCCGTC ACTTGGCCTG 
TCACTTGAGG AGGAGGAGGA GCAGAGGAGG TGGAAGAAGC AGCTTAGGGA GGGCAGGGAG 
AGAGAGGCCA GGCAGGCACT GCTGATACAT CGGCTGCAGA ACAAGGTTCT GGAGTACAGA 
GATCAGTGCC AGCATTTGGA CCTGCAGTTG CAAGATGAAC GGAGGAAGAC GCTCCACATG 
GAGGGGGTCC GGGATGAACA TAGCAACTCT CTGGAAAGCG CCCTCATCAG GCTGGAGGAG 
GAGCAGCACA GGTCCGTAAG TCTGGCTGAC ACCAACTCTC TTCTGCGGGA GCAGCTCAGC 
CAATCTGAGA AGTTCAACCA GGAGCTTAAG GAGGAACTCC ACAGACTGAC AGCTGATTGG 
ATCAGATCCA CGGAGAAGGC GGAGCAAAGA GAAACTGATT GGCAGAGAGA GAAAGAGGAT 
CAGCGGGGTC ACGTGGGTCA GCAGCAGAAC AACTGGCTGT CAGTTTGGAG ATCCGTGGTG 
GCTCTGAGGA GACAATGTCA CAGCGTGAAA ACAGCTGCCG ACAGAGACCT GTGGGAGCTC 
AGGGCCGAGT TTTCCCGTCT CTCCTCAACA CTCCTCTCCA GCTGTGACTC TGTCTGCTCC 
ACCCAGAGCC TGGCGGCTCT TCACAAGCCT TTGTCTTCAA CTTTGCTGCC TCTTCCTTCC 
CCACCATCCA GTTCTCCTCC TCTATCCTCA ACACTGATCC TCCCTCCTCC TCCTCCAGAC 
TCCTGCTCCA CTCAGGGATC CTTCTCCATG GGGGAGCTAG AACTCAAAGA GGAGGAGGGA 
GAAGGTTCTG AGCCTAAGCT CTATGAAAAG ACTGAAGCTT TGCAGCAGAG GACTGTGGAG 
CTTGCTCGCT CACTGCAGGA TGAGAGAGAG GAGAAGGAGA GGGAGGTGGA AAGACATCAA 
GAAACTGCAA GGAGACTCCA GTCTGTCAGT CAGGCTGTGA TCAGACTGGC CAGAGTCGTC 
AGCAGCAGAA GTCGGCCAGC GAGCACCTCC CCAGAAGACG TCCTGCGGGC GGATTTGTCC 
TCCATGCTGG CTGTGCTCTC ACAGGCGGAA AGCGTCCTGC TGAGGAGGAG CGAGGAGCTG 
CAGGGGGCGG AGCTAAACCT GCGACGTCTC GGTGAGGTGA GAACCAGCTT GCAGCTGCAG 
GTGAAGCAGC TTCAGGACGA CCACCAGCAG CTTCTGGCAC GCTCACAACA CACACAGCAG 
GAGCTCACGC ACACACAGAG CGTCCTCAGC AGCGAGAAAA AGGTCTCCAG CTCCCTCCGC 
TTGCAGCTGG AGGAGGTGCA AAGAAGAGAG GAGGAGGTGA AGAGAGAGAA CCACAGACTG 
AGGAGAGAGA GAGACAGAGA GGAAGAGAAG AATCTGCAAC TGGAAACAGA CTCACATCGA 
CGGGTGGAGG CGGAGCTCCT AGAAAGTGTC CAGCTGACGG AGAGAGAACA TCTGCACCAG 
ATGGAGCTCC TCAGTCTGAA GGGGGCGCTG GAGAGAGAAC AGCTGGACAG GCAGAAAGCA 
GAGGAAGAGG CAGCAGGCTC TAGGGATGCC CTACAGCAGT GCAGGGGAAG CATACTCCGC 
CTCTCATCCT CAGAAAGCCT GCTGAGGCGG GATCTGGAGG AGAGTCGAGA TGCTTTGAAG 
AAGGTTTCTG CTCTTAACTC TGCTCTGGCC TCAGACAAGA GAGCACTGAA CCAGCAGCTG 
CTGCAGCTGG AAGGCAAACT GTCAGAGAGC CAATCGCAGC TGCAAGCTGT GAGTTCAGAG 
TTCACCTGTC TGCAGAGAGA CGTCAGACCT CTCAGACTGC AGAGGGATCA GGATGCCGAC 
GTCATCCGTC AGCTGAAAGA GCAGCAGGTG GAGGCTGAGA AAAACCTGGA GAGAAAGGAG 
AGAGAGCTTC AGCTATTGGA GGAGGAGAGA ACAAGTGGTG AACAACAGCT GGAGGAGCTT 
CGCTCTCATC ACGTCTCGCT GTGCCAGGAG CTGAAGGAGT TGCAGGAGGA GTTGCAACAG 
GCGAAACAGG AGGTTAGGAT TCTGAATTGG CAGCAGGAGG AGCAGCAAAG GGAGAGCAGG 
AGCCGGCAGG AGGAGCTCCA GAATGAGCAT AGACAGCGGG CGCAGCTGGA GGAGGAGCTG 
CATGAGCTCA GGCCTCTGTC AGTGTCCCTG CAGCTGCAGC TCAGCCAGCA GCAGCAGCTC 
CTCTCCCAGG TCGAGGTGGA CCGCTGTCAG CTGAGCACTC GCCTCCATGC GCTGCAGGAG 
GCCAAGCTCA CCTTACAGGG TGAAATCAAA TGTCTGAGAG GGGAGCTGCA GAGAGAGACA 
GCCAGCAGAG AGAGAAGTGA GGGCGAGAAG GAGGTGCTGC AGGAAGACCT GCAGAGACTG 
ACGGAAGAGG TGCAGGAGCT GAGGAGAAGA AAGGCAGAAA AGGAAGAGGA GAAAAAGATG 
TGCCAAAGAG AACGAGAGAC ACTGAGGGAG GAATTAAGGC TGAGAGACGG GGAGCTGGAA 
GCCCTGAAGA CACGCACTGA AAGGATGGAA GAGGAGCTGG AAGAAAAGGG ACAACAGGTG 
GAGAGACTGA AGGAGGAGGT GACACAACGA GCTACCCAAG TCAGCTTCCT AAATGAGAGA 
ATAGTTGGCT TGGAAGAGGA AAAGAAGATT CTGCTGGAGG AGGCAAAGAG TACTGAGGTC 
AGACTGCTGG AGGAGGAGGC GAAAAGGAAA GACGAGGAGG TGCAAAGAGA TGGGGAGACT 
GAAGCTCTCT GTGACAGAAT AGAGAAATTA GAAAGAGAGA AGGAAGAAAT AGATAAGGAG 
GTGCACCAAA TGAAGAGAAA AGCACAGGAG CAAGAGGAGA GATGGAGGTC AAATGATGAG 
GAGAGCCAGA ATGAGATAAA CAGACTAAGG GGAGAAAACT CTTCCCTCCA GGAGCACAGA 
AAGTCAGAGA AGCGACAAAT AGAGAAACTG CAAAATGAAA AAATGGAGGT GCTGGAGAGG 
CTACAAGAGA AAGTTGGAGA GGTGGAACTG ATGAAGGTAA AACTAAGTGT GGCTCAGGAG 
GAGAGTGAGG AGCTGAGGGA GGAGGTGCAG AGGAGGGAGC GCAGCCTGGA GAAACAGATA 
TGTGCAGTAC GAGAAAGGGA GGAGGAAATG GAGAACCTGA GGGGGCGGCT GAGGTTGGTG 
AAGGAGCGGG AGGCGGAAGC AGAGAGAGAG CTCCAGGAAA CTTCTGACAA ACTGAAAAAA 
AAAGAGGCGA GGGAGGAACA CCTGGAGGTG CTGCTTAGAG AAAACCGGGA ACTTTTGAAG 
CAGGAGGAAA AAGGACGAGA AGAAAGAGAA GACAGGATCT GCTCCTTGAT CAAACAATTG 
CAGGAGGCCC AGGCTGTTAA CCTGGGAGCA AAAAGAAGAA GCACAGAAAG GCAGAAGGCC 
CATGACAGAC TGCAGGAGGA GGTGAAGGAC TGGCAGGAGC AAGCAGAACA AAGTGTGAAG 
GAGGTGGCAA AACTCAAAGA AATCCTGAAG GGGAGAAATG AGGAGGTGAA CCAGCTGAAA 
GCAATGCTTG AAGAAAAAGA AGAGGAGTGG AAAGAGCAGG AGGCTTTGAG TAAAACAGAG 
AAAGAAAGGA GGAGAAGGCT GGAAAACAAG CTGGTCGAGG TGGAGGAAGA GAACACCAGG 
TTAGAGAAGG AGGTGAGACT GATGCAGGCA GTTGGAGATA AAGATAGGGA GGCTCTGGCA 
AAGGCTGGAG AGGAGTTAAA AACATTGAAG GACAACCTGG TAAAAAGTGA AAACAAAGAG 
AGGGAGCTGG GAGAAACTGT GAGAAGAATG GAAGAGGAGA AGATGATGCT GAGTAGCAAT 
TTGAGAGAGA TGGAGGACGA GATCAAAGCA GAGAAGGAGC AGTTGTGGAG AGTAAGGAAA 
GAGAGGAGCA GTCTGGAGGA CAAACTAAAA GAAAAAGAGA GAGAAATAGA TGAGTGCAGG 
GATTTGCTTA GAAGTCAGGA GGAAAAACTT GAGGAGGTGA AAAAGATGGA TAGAAAAACA 
TTGGAGAGTG AAAGAAATCG GTATCTACTG TTGGAGGAAA AAGGGAAGAC CAGGGTTAAA 
AAGCTTGAGG AGGAGAAGGA GGACCTTTTA CTGCAGTTAA TGAAAAGGGA AAAAGAAGTG 
ACAGAACTCC AAGAGGAGGT GCAGAGGCAG AGGATCACTG CAAGCAAGGA GCTCAATGAA 
AAGAAAGAGG AGGCATTTAA TGTACAAAGG GAATGTGAGG AGGCGCAGGA AAAGCTAAAA 
TCATTGAAAA GGGAAATGAT TTATCTCCAA GAGGAGGTGA AACAAAAGCA AGAAGAGAAT 
GAGGAGGTGC AGGAAAAACT GCAATCATTG AAGAGGGAAA TGATTTATCT CCAAGAGGAG 
GTGAAACAAA AGCAAGAAGA GAATGAGGAG GTGCAGGAAA AACTGCAATC ATTGAAGAGG 
GAAATGATTT ATCTCCAGGA GGAGGTGAAA CAAAAGCAAG AAGACAATGA GGAGGTGCAG 
GAAAAACTCC AATCATTAAA AAGCGAAATG ATTTATCTCC AAGAGGAGGT GAAACAAAAG 
CAAAAAGAGA ATGAGGAGAC ACAGGAGAAA ATGACCGTGT TAAAGGAGGT GCACCAGAAA 
CATTTAAAGA AAGAGGAAGA GTGGAGAACT AACGATATGA TGAGCAGAAA TGACACCCAG 
GAGCTACAGA GGCAGAAAGA TGAGGCTCAA GAGGAGCTGC AGAGGAGCAG AAAGGAGATC 
TACATACTTA GAGAGGAGCT AGAAAAGGAA CAAAGAGAAA AAAAGGACCT TCAAGATGAG 
ATGAAAAAGA GGAGTGAAGA GGCTGGATTG CTAAGACAGA AAATAGAGGA GGAGTTCAGC 
AGCACCAAGA AGAGGGAGGA GACAGAAAAA CAAACAAGAG AGAAGACTGA GATAGAGTTG 
GCCGCTCTAA AGGAGGAGCT GCAAAGGGAA CACAGGGAGA AATTGGAGAC AGTGGAGGAG 
CTGAAGAAGA GGAGAGAGGA TGTCACTGTG CTTGAAGGAG AGGTGAAGGA GGAGTGCATG 
AAGAAGGAGG AGGTGCAGGA AGATCTAAGA AGAGTGCAGC ACAGCTTGGA GCAGATGTCT 
CACAGTCTGA GCTCCCTTCA GAGTCAGGTG TCAGAGCTGA GTATCAGCAA GGAGCGAGTA 
GAGGTGGAGA AGCAGCAGAT GAGAGAAGGC CTGCAGGTGG CGCTCAAGGA GATGGACTTG 
CTGAAAGTTC TCCTCCAGGA AAGCCATGCA GAAGGAGAAC TGCTGAGGAG CACACTGGAG 
GAGAAGAAGA AGGAGATGGG GAGGGTTCGA CAGGAGAACC TGCAGATGGA AGAGAGGCAA 
GAGGAGCTGG AGGAGCTGAG AGCCAGGGTC AAAGCCCTGG AGAGAAGAAG GACGGAGATG 
GCAGAGGAGC TAGATAGGGC AGATGAGAGG AGGAGGGAGG CTGAGGAGTG GTGGAGGAGC 
AAAGTGAAGG AAGTAGAACG GCAGAAGGGG ACGATGCAAA GCACTCTTTA CACAGAAATA 
CAGACGCAAG GGGAGCTACT GAAGGAGTCG AGGTCCAAGC TTCAGGACAG TTTGAGGGAG 
CTGGAGGAGG CGAGAATGGA GCTTAGCCGG AACAGAGCCT TGTTGGAGGA GCAGAAAACT 
TTCACGATTG CTGACAGAGA GGATTCGGGG GAGCAGAGGA GCAGAGGCAG CCAAGAGGAA 
AGAGGGGAGG TGAAGCAGGA GGAGATCAGG AGGCAGCTCC AGCAGAGGGA GGCTCAGGTG 
GACAGTTTGA CTCTCAGAGT CCAGGAGCTG CAGGAAGACA GAAATCAGCT CCGATTGGCT 
CTGGAACAAA CTGAGGCGCA GCTGAAAAAC GTCGCCACCC AGGTGGAGCA GAGCACGGGG 
GCGGAGCCTA ACCCAGGAGA GACTGTAGGG GGCAGACTGG AGGTCCTGCA GAGACTGGTG 
GCTGAACTGG AGCTCCAACG GACACGACTG CAGAAGAAGA ACTTGAATCT AGAAAAGCAA 
AAAGACAGAC TGAAGAAAGA CAGAGACTTT CTGAGAGACA CCCTGAGACA GGTGGAGCTG 
GAGCGTGGGA GGCTCAGCCA GCAGCTGACA GACAGCAGAG GATCTCAGGT GGAACAGGTG 
TGCGCTAATG CCACAGAAGA AGATCGTCTG CGCAGCAAAG TGAAGGAGCT GGAGGAGCAG 
GTTAGTCAGC TTCGTCTTTC TTTAGCTATG GACCAGCAGC AGAAGGCGGA GTTTATCCAG 
CAGTCATCCA GAAACAGCCA ATGGCTGCTC TCACTAAGAC GGGACCTCAA TGATTCGCTG 
TCTGCTGTGA CCCGTCTACC AATCCCGTCT GTCCTGGAGT CTGAGACACA GAGGTTGGAC 
CGCAGCCTGA GGGAGGAGGA GCTTAGGATT TCCTTGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101166347 ( XM_023950730.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806498.1 Oryzias latipes trichohyalin (LOC101166347), transcript variant X3, mRNA.

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CloneID	OJa113159
Clone ID Related Accession (Same CDS sequence)	XM_023950730.1
Accession Version	XM_023950730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	trichohyalin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG GGTTGCTGAT TGGCTGGCAG CAGCAGAAGG AGGAGCTCGA GCGGGAGGTA 
TGCCGTCTGC AGGAGGAGCT GGCAGAGAGT CGAGCGGAGC GGGAGGAGTT GGAGTCCAGA 
ACCCGAGCTC TGCAGGACAG GCTCCGTCAG ACCATACATC CGTCACTTGG CCTGTCACTT 
GAGGAGGAGG AGGAGCAGAG GAGGTGGAAG AAGCAGCTTA GGGAGGGCAG GGAGAGAGAG 
GCCAGGCAGG CACTGCTGAT ACATCGGCTG CAGAACAAGG TTCTGGAGTA CAGAGATCAG 
TGCCAGCATT TGGACCTGCA GTTGCAAGAT GAACGGAGGA AGACGCTCCA CATGGAGGGG 
GTCCGGGATG AACATAGCAA CTCTCTGGAA AGCGCCCTCA TCAGGCTGGA GGAGGAGCAG 
CACAGGTCCG TAAGTCTGGC TGACACCAAC TCTCTTCTGC GGGAGCAGCT CAGCCAATCT 
GAGAAGTTCA ACCAGGAGCT TAAGGAGGAA CTCCACAGAC TGACAGCTGA TTGGATCAGA 
TCCACGGAGA AGGCGGAGCA AAGAGAAACT GATTGGCAGA GAGAGAAAGA GGATCAGCGG 
GGTCACGTGG GTCAGCAGCA GAACAACTGG CTGTCAGTTT GGAGATCCGT GGTGGCTCTG 
AGGAGACAAT GTCACAGCGT GAAAACAGCT GCCGACAGAG ACCTGTGGGA GCTCAGGGCC 
GAGTTTTCCC GTCTCTCCTC AACACTCCTC TCCAGCTGTG ACTCTGTCTG CTCCACCCAG 
AGCCTGGCGG CTCTTCACAA GCCTTTGTCT TCAACTTTGC TGCCTCTTCC TTCCCCACCA 
TCCAGTTCTC CTCCTCTATC CTCAACACTG ATCCTCCCTC CTCCTCCTCC AGACTCCTGC 
TCCACTCAGG GATCCTTCTC CATGGGGGAG CTAGAACTCA AAGAGGAGGA GGGAGAAGGT 
TCTGAGCCTA AGCTCTATGA AAAGACTGAA GCTTTGCAGC AGAGGACTGT GGAGCTTGCT 
CGCTCACTGC AGGATGAGAG AGAGGAGAAG GAGAGGGAGG TGGAAAGACA TCAAGAAACT 
GCAAGGAGAC TCCAGTCTGT CAGTCAGGCT GTGATCAGAC TGGCCAGAGT CGTCAGCAGC 
AGAAGTCGGC CAGCGAGCAC CTCCCCAGAA GACGTCCTGC GGGCGGATTT GTCCTCCATG 
CTGGCTGTGC TCTCACAGGC GGAAAGCGTC CTGCTGAGGA GGAGCGAGGA GCTGCAGGGG 
GCGGAGCTAA ACCTGCGACG TCTCGGTGAG GTGAGAACCA GCTTGCAGCT GCAGGTGAAG 
CAGCTTCAGG ACGACCACCA GCAGCTTCTG GCACGCTCAC AACACACACA GCAGGAGCTC 
ACGCACACAC AGAGCGTCCT CAGCAGCGAG AAAAAGGTCT CCAGCTCCCT CCGCTTGCAG 
CTGGAGGAGG TGCAAAGAAG AGAGGAGGAG GTGAAGAGAG AGAACCACAG ACTGAGGAGA 
GAGAGAGACA GAGAGGAAGA GAAGAATCTG CAACTGGAAA CAGACTCACA TCGACGGGTG 
GAGGCGGAGC TCCTAGAAAG TGTCCAGCTG ACGGAGAGAG AACATCTGCA CCAGATGGAG 
CTCCTCAGTC TGAAGGGGGC GCTGGAGAGA GAACAGCTGG ACAGGCAGAA AGCAGAGGAA 
GAGGCAGCAG GCTCTAGGGA TGCCCTACAG CAGTGCAGGG GAAGCATACT CCGCCTCTCA 
TCCTCAGAAA GCCTGCTGAG GCGGGATCTG GAGGAGAGTC GAGATGCTTT GAAGAAGGTT 
TCTGCTCTTA ACTCTGCTCT GGCCTCAGAC AAGAGAGCAC TGAACCAGCA GCTGCTGCAG 
CTGGAAGGCA AACTGTCAGA GAGCCAATCG CAGCTGCAAG CTGTGAGTTC AGAGTTCACC 
TGTCTGCAGA GAGACGTCAG ACCTCTCAGA CTGCAGAGGG ATCAGGATGC CGACGTCATC 
CGTCAGCTGA AAGAGCAGCA GGTGGAGGCT GAGAAAAACC TGGAGAGAAA GGAGAGAGAG 
CTTCAGCTAT TGGAGGAGGA GAGAACAAGT GGTGAACAAC AGCTGGAGGA GCTTCGCTCT 
CATCACGTCT CGCTGTGCCA GGAGCTGAAG GAGTTGCAGG AGGAGTTGCA ACAGGCGAAA 
CAGGAGGTTA GGATTCTGAA TTGGCAGCAG GAGGAGCAGC AAAGGGAGAG CAGGAGCCGG 
CAGGAGGAGC TCCAGAATGA GCATAGACAG CGGGCGCAGC TGGAGGAGGA GCTGCATGAG 
CTCAGGCCTC TGTCAGTGTC CCTGCAGCTG CAGCTCAGCC AGCAGCAGCA GCTCCTCTCC 
CAGGTCGAGG TGGACCGCTG TCAGCTGAGC ACTCGCCTCC ATGCGCTGCA GGAGGCCAAG 
CTCACCTTAC AGGGTGAAAT CAAATGTCTG AGAGGGGAGC TGCAGAGAGA GACAGCCAGC 
AGAGAGAGAA GTGAGGGCGA GAAGGAGGTG CTGCAGGAAG ACCTGCAGAG ACTGACGGAA 
GAGGTGCAGG AGCTGAGGAG AAGAAAGGCA GAAAAGGAAG AGGAGAAAAA GATGTGCCAA 
AGAGAACGAG AGACACTGAG GGAGGAATTA AGGCTGAGAG ACGGGGAGCT GGAAGCCCTG 
AAGACACGCA CTGAAAGGAT GGAAGAGGAG CTGGAAGAAA AGGGACAACA GGTGGAGAGA 
CTGAAGGAGG AGGTGACACA ACGAGCTACC CAAGTCAGCT TCCTAAATGA GAGAATAGTT 
GGCTTGGAAG AGGAAAAGAA GATTCTGCTG GAGGAGGCAA AGAGTACTGA GGTCAGACTG 
CTGGAGGAGG AGGCGAAAAG GAAAGACGAG GAGGTGCAAA GAGATGGGGA GACTGAAGCT 
CTCTGTGACA GAATAGAGAA ATTAGAAAGA GAGAAGGAAG AAATAGATAA GGAGGTGCAC 
CAAATGAAGA GAAAAGCACA GGAGCAAGAG GAGAGATGGA GGTCAAATGA TGAGGAGAGC 
CAGAATGAGA TAAACAGACT AAGGGGAGAA AACTCTTCCC TCCAGGAGCA CAGAAAGTCA 
GAGAAGCGAC AAATAGAGAA ACTGCAAAAT GAAAAAATGG AGGTGCTGGA GAGGCTACAA 
GAGAAAGTTG GAGAGGTGGA ACTGATGAAG GTAAAACTAA GTGTGGCTCA GGAGGAGAGT 
GAGGAGCTGA GGGAGGAGGT GCAGAGGAGG GAGCGCAGCC TGGAGAAACA GATATGTGCA 
GTACGAGAAA GGGAGGAGGA AATGGAGAAC CTGAGGGGGC GGCTGAGGTT GGTGAAGGAG 
CGGGAGGCGG AAGCAGAGAG AGAGCTCCAG GAAACTTCTG ACAAACTGAA AAAAAAAGAG 
GCGAGGGAGG AACACCTGGA GGTGCTGCTT AGAGAAAACC GGGAACTTTT GAAGCAGGAG 
GAAAAAGGAC GAGAAGAAAG AGAAGACAGG ATCTGCTCCT TGATCAAACA ATTGCAGGAG 
GCCCAGGCTG TTAACCTGGG AGCAAAAAGA AGAAGCACAG AAAGGCAGAA GGCCCATGAC 
AGACTGCAGG AGGAGGTGAA GGACTGGCAG GAGCAAGCAG AACAAAGTGT GAAGGAGGTG 
GCAAAACTCA AAGAAATCCT GAAGGGGAGA AATGAGGAGG TGAACCAGCT GAAAGCAATG 
CTTGAAGAAA AAGAAGAGGA GTGGAAAGAG CAGGAGGCTT TGAGTAAAAC AGAGAAAGAA 
AGGAGGAGAA GGCTGGAAAA CAAGCTGGTC GAGGTGGAGG AAGAGAACAC CAGGTTAGAG 
AAGGAGGTGA GACTGATGCA GGCAGTTGGA GATAAAGATA GGGAGGCTCT GGCAAAGGCT 
GGAGAGGAGT TAAAAACATT GAAGGACAAC CTGGTAAAAA GTGAAAACAA AGAGAGGGAG 
CTGGGAGAAA CTGTGAGAAG AATGGAAGAG GAGAAGATGA TGCTGAGTAG CAATTTGAGA 
GAGATGGAGG ACGAGATCAA AGCAGAGAAG GAGCAGTTGT GGAGAGTAAG GAAAGAGAGG 
AGCAGTCTGG AGGACAAACT AAAAGAAAAA GAGAGAGAAA TAGATGAGTG CAGGGATTTG 
CTTAGAAGTC AGGAGGAAAA ACTTGAGGAG GTGAAAAAGA TGGATAGAAA AACATTGGAG 
AGTGAAAGAA ATCGGTATCT ACTGTTGGAG GAAAAAGGGA AGACCAGGGT TAAAAAGCTT 
GAGGAGGAGA AGGAGGACCT TTTACTGCAG TTAATGAAAA GGGAAAAAGA AGTGACAGAA 
CTCCAAGAGG AGGTGCAGAG GCAGAGGATC ACTGCAAGCA AGGAGCTCAA TGAAAAGAAA 
GAGGAGGCAT TTAATGTACA AAGGGAATGT GAGGAGGCGC AGGAAAAGCT AAAATCATTG 
AAAAGGGAAA TGATTTATCT CCAAGAGGAG GTGAAACAAA AGCAAGAAGA GAATGAGGAG 
GTGCAGGAAA AACTGCAATC ATTGAAGAGG GAAATGATTT ATCTCCAAGA GGAGGTGAAA 
CAAAAGCAAG AAGAGAATGA GGAGGTGCAG GAAAAACTGC AATCATTGAA GAGGGAAATG 
ATTTATCTCC AGGAGGAGGT GAAACAAAAG CAAGAAGACA ATGAGGAGGT GCAGGAAAAA 
CTCCAATCAT TAAAAAGCGA AATGATTTAT CTCCAAGAGG AGGTGAAACA AAAGCAAAAA 
GAGAATGAGG AGACACAGGA GAAAATGACC GTGTTAAAGG AGGTGCACCA GAAACATTTA 
AAGAAAGAGG AAGAGTGGAG AACTAACGAT ATGATGAGCA GAAATGACAC CCAGGAGCTA 
CAGAGGCAGA AAGATGAGGC TCAAGAGGAG CTGCAGAGGA GCAGAAAGGA GATCTACATA 
CTTAGAGAGG AGCTAGAAAA GGAACAAAGA GAAAAAAAGG ACCTTCAAGA TGAGATGAAA 
AAGAGGAGTG AAGAGGCTGG ATTGCTAAGA CAGAAAATAG AGGAGGAGTT CAGCAGCACC 
AAGAAGAGGG AGGAGACAGA AAAACAAACA AGAGAGAAGA CTGAGATAGA GTTGGCCGCT 
CTAAAGGAGG AGCTGCAAAG GGAACACAGG GAGAAATTGG AGACAGTGGA GGAGCTGAAG 
AAGAGGAGAG AGGATGTCAC TGTGCTTGAA GGAGAGGTGA AGGAGGAGTG CATGAAGAAG 
GAGGAGGTGC AGGAAGATCT AAGAAGAGTG CAGCACAGCT TGGAGCAGAT GTCTCACAGT 
CTGAGCTCCC TTCAGAGTCA GGTGTCAGAG CTGAGTATCA GCAAGGAGCG AGTAGAGGTG 
GAGAAGCAGC AGATGAGAGA AGGCCTGCAG GTGGCGCTCA AGGAGATGGA CTTGCTGAAA 
GTTCTCCTCC AGGAAAGCCA TGCAGAAGGA GAACTGCTGA GGAGCACACT GGAGGAGAAG 
AAGAAGGAGA TGGGGAGGGT TCGACAGGAG AACCTGCAGA TGGAAGAGAG GCAAGAGGAG 
CTGGAGGAGC TGAGAGCCAG GGTCAAAGCC CTGGAGAGAA GAAGGACGGA GATGGCAGAG 
GAGCTAGATA GGGCAGATGA GAGGAGGAGG GAGGCTGAGG AGTGGTGGAG GAGCAAAGTG 
AAGGAAGTAG AACGGCAGAA GGGGACGATG CAAAGCACTC TTTACACAGA AATACAGACG 
CAAGGGGAGC TACTGAAGGA GTCGAGGTCC AAGCTTCAGG ACAGTTTGAG GGAGCTGGAG 
GAGGCGAGAA TGGAGCTTAG CCGGAACAGA GCCTTGTTGG AGGAGCAGAA AACTTTCACG 
ATTGCTGACA GAGAGGATTC GGGGGAGCAG AGGAGCAGAG GCAGCCAAGA GGAAAGAGGG 
GAGGTGAAGC AGGAGGAGAT CAGGAGGCAG CTCCAGCAGA GGGAGGCTCA GGTGGACAGT 
TTGACTCTCA GAGTCCAGGA GCTGCAGGAA GACAGAAATC AGCTCCGATT GGCTCTGGAA 
CAAACTGAGG CGCAGCTGAA AAACGTCGCC ACCCAGGTGG AGCAGAGCAC GGGGGCGGAG 
CCTAACCCAG GAGAGACTGT AGGGGGCAGA CTGGAGGTCC TGCAGAGACT GGTGGCTGAA 
CTGGAGCTCC AACGGACACG ACTGCAGAAG AAGAACTTGA ATCTAGAAAA GCAAAAAGAC 
AGACTGAAGA AAGACAGAGA CTTTCTGAGA GACACCCTGA GACAGGTGGA GCTGGAGCGT 
GGGAGGCTCA GCCAGCAGCT GACAGACAGC AGAGGATCTC AGGTGGAACA GGTGTGCGCT 
AATGCCACAG AAGAAGATCG TCTGCGCAGC AAAGTGAAGG AGCTGGAGGA GCAGGTTAGT 
CAGCTTCGTC TTTCTTTAGC TATGGACCAG CAGCAGAAGG CGGAGTTTAT CCAGCAGTCA 
TCCAGAAACA GCCAATGGCT GCTCTCACTA AGACGGGACC TCAATGATTC GCTGTCTGCT 
GTGACCCGTC TACCAATCCC GTCTGTCCTG GAGTCTGAGA CACAGAGGTT GGACCGCAGC 
CTGAGGGAGG AGGAGCTTAG GATTTCCTTG AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806498.1
CDS	321..6836
Translation	MDSGLLIGWQQQKEELEREVCRLQEELAESRAEREELESRTRALQDRLRQTIHPSLGLSLEEEEEQRRWKKQLREGREREARQALLIHRLQNKVLEYRDQCQHLDLQLQDERRKTLHMEGVRDEHSNSLESALIRLEEEQHRSVSLADTNSLLREQLSQSEKFNQELKEELHRLTADWIRSTEKAEQRETDWQREKEDQRGHVGQQQNNWLSVWRSVVALRRQCHSVKTAADRDLWELRAEFSRLSSTLLSSCDSVCSTQSLAALHKPLSSTLLPLPSPPSSSPPLSSTLILPPPPPDSCSTQGSFSMGELELKEEEGEGSEPKLYEKTEALQQRTVELARSLQDEREEKEREVERHQETARRLQSVSQAVIRLARVVSSRSRPASTSPEDVLRADLSSMLAVLSQAESVLLRRSEELQGAELNLRRLGEVRTSLQLQVKQLQDDHQQLLARSQHTQQELTHTQSVLSSEKKVSSSLRLQLEEVQRREEEVKRENHRLRRERDREEEKNLQLETDSHRRVEAELLESVQLTEREHLHQMELLSLKGALEREQLDRQKAEEEAAGSRDALQQCRGSILRLSSSESLLRRDLEESRDALKKVSALNSALASDKRALNQQLLQLEGKLSESQSQLQAVSSEFTCLQRDVRPLRLQRDQDADVIRQLKEQQVEAEKNLERKERELQLLEEERTSGEQQLEELRSHHVSLCQELKELQEELQQAKQEVRILNWQQEEQQRESRSRQEELQNEHRQRAQLEEELHELRPLSVSLQLQLSQQQQLLSQVEVDRCQLSTRLHALQEAKLTLQGEIKCLRGELQRETASRERSEGEKEVLQEDLQRLTEEVQELRRRKAEKEEEKKMCQRERETLREELRLRDGELEALKTRTERMEEELEEKGQQVERLKEEVTQRATQVSFLNERIVGLEEEKKILLEEAKSTEVRLLEEEAKRKDEEVQRDGETEALCDRIEKLEREKEEIDKEVHQMKRKAQEQEERWRSNDEESQNEINRLRGENSSLQEHRKSEKRQIEKLQNEKMEVLERLQEKVGEVELMKVKLSVAQEESEELREEVQRRERSLEKQICAVREREEEMENLRGRLRLVKEREAEAERELQETSDKLKKKEAREEHLEVLLRENRELLKQEEKGREEREDRICSLIKQLQEAQAVNLGAKRRSTERQKAHDRLQEEVKDWQEQAEQSVKEVAKLKEILKGRNEEVNQLKAMLEEKEEEWKEQEALSKTEKERRRRLENKLVEVEEENTRLEKEVRLMQAVGDKDREALAKAGEELKTLKDNLVKSENKERELGETVRRMEEEKMMLSSNLREMEDEIKAEKEQLWRVRKERSSLEDKLKEKEREIDECRDLLRSQEEKLEEVKKMDRKTLESERNRYLLLEEKGKTRVKKLEEEKEDLLLQLMKREKEVTELQEEVQRQRITASKELNEKKEEAFNVQRECEEAQEKLKSLKREMIYLQEEVKQKQEENEEVQEKLQSLKREMIYLQEEVKQKQEENEEVQEKLQSLKREMIYLQEEVKQKQEDNEEVQEKLQSLKSEMIYLQEEVKQKQKENEETQEKMTVLKEVHQKHLKKEEEWRTNDMMSRNDTQELQRQKDEAQEELQRSRKEIYILREELEKEQREKKDLQDEMKKRSEEAGLLRQKIEEEFSSTKKREETEKQTREKTEIELAALKEELQREHREKLETVEELKKRREDVTVLEGEVKEECMKKEEVQEDLRRVQHSLEQMSHSLSSLQSQVSELSISKERVEVEKQQMREGLQVALKEMDLLKVLLQESHAEGELLRSTLEEKKKEMGRVRQENLQMEERQEELEELRARVKALERRRTEMAEELDRADERRREAEEWWRSKVKEVERQKGTMQSTLYTEIQTQGELLKESRSKLQDSLRELEEARMELSRNRALLEEQKTFTIADREDSGEQRSRGSQEERGEVKQEEIRRQLQQREAQVDSLTLRVQELQEDRNQLRLALEQTEAQLKNVATQVEQSTGAEPNPGETVGGRLEVLQRLVAELELQRTRLQKKNLNLEKQKDRLKKDRDFLRDTLRQVELERGRLSQQLTDSRGSQVEQVCANATEEDRLRSKVKELEEQVSQLRLSLAMDQQQKAEFIQQSSRNSQWLLSLRRDLNDSLSAVTRLPIPSVLESETQRLDRSLREEELRISLS

Target ORF information:

RefSeq Version	XM_023950730.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes trichohyalin (LOC101166347), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950730.1

ORF Insert Sequence:

ATGGATTCTG GGTTGCTGAT TGGCTGGCAG CAGCAGAAGG AGGAGCTCGA GCGGGAGGTA 
TGCCGTCTGC AGGAGGAGCT GGCAGAGAGT CGAGCGGAGC GGGAGGAGTT GGAGTCCAGA 
ACCCGAGCTC TGCAGGACAG GCTCCGTCAG ACCATACATC CGTCACTTGG CCTGTCACTT 
GAGGAGGAGG AGGAGCAGAG GAGGTGGAAG AAGCAGCTTA GGGAGGGCAG GGAGAGAGAG 
GCCAGGCAGG CACTGCTGAT ACATCGGCTG CAGAACAAGG TTCTGGAGTA CAGAGATCAG 
TGCCAGCATT TGGACCTGCA GTTGCAAGAT GAACGGAGGA AGACGCTCCA CATGGAGGGG 
GTCCGGGATG AACATAGCAA CTCTCTGGAA AGCGCCCTCA TCAGGCTGGA GGAGGAGCAG 
CACAGGTCCG TAAGTCTGGC TGACACCAAC TCTCTTCTGC GGGAGCAGCT CAGCCAATCT 
GAGAAGTTCA ACCAGGAGCT TAAGGAGGAA CTCCACAGAC TGACAGCTGA TTGGATCAGA 
TCCACGGAGA AGGCGGAGCA AAGAGAAACT GATTGGCAGA GAGAGAAAGA GGATCAGCGG 
GGTCACGTGG GTCAGCAGCA GAACAACTGG CTGTCAGTTT GGAGATCCGT GGTGGCTCTG 
AGGAGACAAT GTCACAGCGT GAAAACAGCT GCCGACAGAG ACCTGTGGGA GCTCAGGGCC 
GAGTTTTCCC GTCTCTCCTC AACACTCCTC TCCAGCTGTG ACTCTGTCTG CTCCACCCAG 
AGCCTGGCGG CTCTTCACAA GCCTTTGTCT TCAACTTTGC TGCCTCTTCC TTCCCCACCA 
TCCAGTTCTC CTCCTCTATC CTCAACACTG ATCCTCCCTC CTCCTCCTCC AGACTCCTGC 
TCCACTCAGG GATCCTTCTC CATGGGGGAG CTAGAACTCA AAGAGGAGGA GGGAGAAGGT 
TCTGAGCCTA AGCTCTATGA AAAGACTGAA GCTTTGCAGC AGAGGACTGT GGAGCTTGCT 
CGCTCACTGC AGGATGAGAG AGAGGAGAAG GAGAGGGAGG TGGAAAGACA TCAAGAAACT 
GCAAGGAGAC TCCAGTCTGT CAGTCAGGCT GTGATCAGAC TGGCCAGAGT CGTCAGCAGC 
AGAAGTCGGC CAGCGAGCAC CTCCCCAGAA GACGTCCTGC GGGCGGATTT GTCCTCCATG 
CTGGCTGTGC TCTCACAGGC GGAAAGCGTC CTGCTGAGGA GGAGCGAGGA GCTGCAGGGG 
GCGGAGCTAA ACCTGCGACG TCTCGGTGAG GTGAGAACCA GCTTGCAGCT GCAGGTGAAG 
CAGCTTCAGG ACGACCACCA GCAGCTTCTG GCACGCTCAC AACACACACA GCAGGAGCTC 
ACGCACACAC AGAGCGTCCT CAGCAGCGAG AAAAAGGTCT CCAGCTCCCT CCGCTTGCAG 
CTGGAGGAGG TGCAAAGAAG AGAGGAGGAG GTGAAGAGAG AGAACCACAG ACTGAGGAGA 
GAGAGAGACA GAGAGGAAGA GAAGAATCTG CAACTGGAAA CAGACTCACA TCGACGGGTG 
GAGGCGGAGC TCCTAGAAAG TGTCCAGCTG ACGGAGAGAG AACATCTGCA CCAGATGGAG 
CTCCTCAGTC TGAAGGGGGC GCTGGAGAGA GAACAGCTGG ACAGGCAGAA AGCAGAGGAA 
GAGGCAGCAG GCTCTAGGGA TGCCCTACAG CAGTGCAGGG GAAGCATACT CCGCCTCTCA 
TCCTCAGAAA GCCTGCTGAG GCGGGATCTG GAGGAGAGTC GAGATGCTTT GAAGAAGGTT 
TCTGCTCTTA ACTCTGCTCT GGCCTCAGAC AAGAGAGCAC TGAACCAGCA GCTGCTGCAG 
CTGGAAGGCA AACTGTCAGA GAGCCAATCG CAGCTGCAAG CTGTGAGTTC AGAGTTCACC 
TGTCTGCAGA GAGACGTCAG ACCTCTCAGA CTGCAGAGGG ATCAGGATGC CGACGTCATC 
CGTCAGCTGA AAGAGCAGCA GGTGGAGGCT GAGAAAAACC TGGAGAGAAA GGAGAGAGAG 
CTTCAGCTAT TGGAGGAGGA GAGAACAAGT GGTGAACAAC AGCTGGAGGA GCTTCGCTCT 
CATCACGTCT CGCTGTGCCA GGAGCTGAAG GAGTTGCAGG AGGAGTTGCA ACAGGCGAAA 
CAGGAGGTTA GGATTCTGAA TTGGCAGCAG GAGGAGCAGC AAAGGGAGAG CAGGAGCCGG 
CAGGAGGAGC TCCAGAATGA GCATAGACAG CGGGCGCAGC TGGAGGAGGA GCTGCATGAG 
CTCAGGCCTC TGTCAGTGTC CCTGCAGCTG CAGCTCAGCC AGCAGCAGCA GCTCCTCTCC 
CAGGTCGAGG TGGACCGCTG TCAGCTGAGC ACTCGCCTCC ATGCGCTGCA GGAGGCCAAG 
CTCACCTTAC AGGGTGAAAT CAAATGTCTG AGAGGGGAGC TGCAGAGAGA GACAGCCAGC 
AGAGAGAGAA GTGAGGGCGA GAAGGAGGTG CTGCAGGAAG ACCTGCAGAG ACTGACGGAA 
GAGGTGCAGG AGCTGAGGAG AAGAAAGGCA GAAAAGGAAG AGGAGAAAAA GATGTGCCAA 
AGAGAACGAG AGACACTGAG GGAGGAATTA AGGCTGAGAG ACGGGGAGCT GGAAGCCCTG 
AAGACACGCA CTGAAAGGAT GGAAGAGGAG CTGGAAGAAA AGGGACAACA GGTGGAGAGA 
CTGAAGGAGG AGGTGACACA ACGAGCTACC CAAGTCAGCT TCCTAAATGA GAGAATAGTT 
GGCTTGGAAG AGGAAAAGAA GATTCTGCTG GAGGAGGCAA AGAGTACTGA GGTCAGACTG 
CTGGAGGAGG AGGCGAAAAG GAAAGACGAG GAGGTGCAAA GAGATGGGGA GACTGAAGCT 
CTCTGTGACA GAATAGAGAA ATTAGAAAGA GAGAAGGAAG AAATAGATAA GGAGGTGCAC 
CAAATGAAGA GAAAAGCACA GGAGCAAGAG GAGAGATGGA GGTCAAATGA TGAGGAGAGC 
CAGAATGAGA TAAACAGACT AAGGGGAGAA AACTCTTCCC TCCAGGAGCA CAGAAAGTCA 
GAGAAGCGAC AAATAGAGAA ACTGCAAAAT GAAAAAATGG AGGTGCTGGA GAGGCTACAA 
GAGAAAGTTG GAGAGGTGGA ACTGATGAAG GTAAAACTAA GTGTGGCTCA GGAGGAGAGT 
GAGGAGCTGA GGGAGGAGGT GCAGAGGAGG GAGCGCAGCC TGGAGAAACA GATATGTGCA 
GTACGAGAAA GGGAGGAGGA AATGGAGAAC CTGAGGGGGC GGCTGAGGTT GGTGAAGGAG 
CGGGAGGCGG AAGCAGAGAG AGAGCTCCAG GAAACTTCTG ACAAACTGAA AAAAAAAGAG 
GCGAGGGAGG AACACCTGGA GGTGCTGCTT AGAGAAAACC GGGAACTTTT GAAGCAGGAG 
GAAAAAGGAC GAGAAGAAAG AGAAGACAGG ATCTGCTCCT TGATCAAACA ATTGCAGGAG 
GCCCAGGCTG TTAACCTGGG AGCAAAAAGA AGAAGCACAG AAAGGCAGAA GGCCCATGAC 
AGACTGCAGG AGGAGGTGAA GGACTGGCAG GAGCAAGCAG AACAAAGTGT GAAGGAGGTG 
GCAAAACTCA AAGAAATCCT GAAGGGGAGA AATGAGGAGG TGAACCAGCT GAAAGCAATG 
CTTGAAGAAA AAGAAGAGGA GTGGAAAGAG CAGGAGGCTT TGAGTAAAAC AGAGAAAGAA 
AGGAGGAGAA GGCTGGAAAA CAAGCTGGTC GAGGTGGAGG AAGAGAACAC CAGGTTAGAG 
AAGGAGGTGA GACTGATGCA GGCAGTTGGA GATAAAGATA GGGAGGCTCT GGCAAAGGCT 
GGAGAGGAGT TAAAAACATT GAAGGACAAC CTGGTAAAAA GTGAAAACAA AGAGAGGGAG 
CTGGGAGAAA CTGTGAGAAG AATGGAAGAG GAGAAGATGA TGCTGAGTAG CAATTTGAGA 
GAGATGGAGG ACGAGATCAA AGCAGAGAAG GAGCAGTTGT GGAGAGTAAG GAAAGAGAGG 
AGCAGTCTGG AGGACAAACT AAAAGAAAAA GAGAGAGAAA TAGATGAGTG CAGGGATTTG 
CTTAGAAGTC AGGAGGAAAA ACTTGAGGAG GTGAAAAAGA TGGATAGAAA AACATTGGAG 
AGTGAAAGAA ATCGGTATCT ACTGTTGGAG GAAAAAGGGA AGACCAGGGT TAAAAAGCTT 
GAGGAGGAGA AGGAGGACCT TTTACTGCAG TTAATGAAAA GGGAAAAAGA AGTGACAGAA 
CTCCAAGAGG AGGTGCAGAG GCAGAGGATC ACTGCAAGCA AGGAGCTCAA TGAAAAGAAA 
GAGGAGGCAT TTAATGTACA AAGGGAATGT GAGGAGGCGC AGGAAAAGCT AAAATCATTG 
AAAAGGGAAA TGATTTATCT CCAAGAGGAG GTGAAACAAA AGCAAGAAGA GAATGAGGAG 
GTGCAGGAAA AACTGCAATC ATTGAAGAGG GAAATGATTT ATCTCCAAGA GGAGGTGAAA 
CAAAAGCAAG AAGAGAATGA GGAGGTGCAG GAAAAACTGC AATCATTGAA GAGGGAAATG 
ATTTATCTCC AGGAGGAGGT GAAACAAAAG CAAGAAGACA ATGAGGAGGT GCAGGAAAAA 
CTCCAATCAT TAAAAAGCGA AATGATTTAT CTCCAAGAGG AGGTGAAACA AAAGCAAAAA 
GAGAATGAGG AGACACAGGA GAAAATGACC GTGTTAAAGG AGGTGCACCA GAAACATTTA 
AAGAAAGAGG AAGAGTGGAG AACTAACGAT ATGATGAGCA GAAATGACAC CCAGGAGCTA 
CAGAGGCAGA AAGATGAGGC TCAAGAGGAG CTGCAGAGGA GCAGAAAGGA GATCTACATA 
CTTAGAGAGG AGCTAGAAAA GGAACAAAGA GAAAAAAAGG ACCTTCAAGA TGAGATGAAA 
AAGAGGAGTG AAGAGGCTGG ATTGCTAAGA CAGAAAATAG AGGAGGAGTT CAGCAGCACC 
AAGAAGAGGG AGGAGACAGA AAAACAAACA AGAGAGAAGA CTGAGATAGA GTTGGCCGCT 
CTAAAGGAGG AGCTGCAAAG GGAACACAGG GAGAAATTGG AGACAGTGGA GGAGCTGAAG 
AAGAGGAGAG AGGATGTCAC TGTGCTTGAA GGAGAGGTGA AGGAGGAGTG CATGAAGAAG 
GAGGAGGTGC AGGAAGATCT AAGAAGAGTG CAGCACAGCT TGGAGCAGAT GTCTCACAGT 
CTGAGCTCCC TTCAGAGTCA GGTGTCAGAG CTGAGTATCA GCAAGGAGCG AGTAGAGGTG 
GAGAAGCAGC AGATGAGAGA AGGCCTGCAG GTGGCGCTCA AGGAGATGGA CTTGCTGAAA 
GTTCTCCTCC AGGAAAGCCA TGCAGAAGGA GAACTGCTGA GGAGCACACT GGAGGAGAAG 
AAGAAGGAGA TGGGGAGGGT TCGACAGGAG AACCTGCAGA TGGAAGAGAG GCAAGAGGAG 
CTGGAGGAGC TGAGAGCCAG GGTCAAAGCC CTGGAGAGAA GAAGGACGGA GATGGCAGAG 
GAGCTAGATA GGGCAGATGA GAGGAGGAGG GAGGCTGAGG AGTGGTGGAG GAGCAAAGTG 
AAGGAAGTAG AACGGCAGAA GGGGACGATG CAAAGCACTC TTTACACAGA AATACAGACG 
CAAGGGGAGC TACTGAAGGA GTCGAGGTCC AAGCTTCAGG ACAGTTTGAG GGAGCTGGAG 
GAGGCGAGAA TGGAGCTTAG CCGGAACAGA GCCTTGTTGG AGGAGCAGAA AACTTTCACG 
ATTGCTGACA GAGAGGATTC GGGGGAGCAG AGGAGCAGAG GCAGCCAAGA GGAAAGAGGG 
GAGGTGAAGC AGGAGGAGAT CAGGAGGCAG CTCCAGCAGA GGGAGGCTCA GGTGGACAGT 
TTGACTCTCA GAGTCCAGGA GCTGCAGGAA GACAGAAATC AGCTCCGATT GGCTCTGGAA 
CAAACTGAGG CGCAGCTGAA AAACGTCGCC ACCCAGGTGG AGCAGAGCAC GGGGGCGGAG 
CCTAACCCAG GAGAGACTGT AGGGGGCAGA CTGGAGGTCC TGCAGAGACT GGTGGCTGAA 
CTGGAGCTCC AACGGACACG ACTGCAGAAG AAGAACTTGA ATCTAGAAAA GCAAAAAGAC 
AGACTGAAGA AAGACAGAGA CTTTCTGAGA GACACCCTGA GACAGGTGGA GCTGGAGCGT 
GGGAGGCTCA GCCAGCAGCT GACAGACAGC AGAGGATCTC AGGTGGAACA GGTGTGCGCT 
AATGCCACAG AAGAAGATCG TCTGCGCAGC AAAGTGAAGG AGCTGGAGGA GCAGGTTAGT 
CAGCTTCGTC TTTCTTTAGC TATGGACCAG CAGCAGAAGG CGGAGTTTAT CCAGCAGTCA 
TCCAGAAACA GCCAATGGCT GCTCTCACTA AGACGGGACC TCAATGATTC GCTGTCTGCT 
GTGACCCGTC TACCAATCCC GTCTGTCCTG GAGTCTGAGA CACAGAGGTT GGACCGCAGC 
CTGAGGGAGG AGGAGCTTAG GATTTCCTTG AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101166347 ( XM_023950731.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806499.1 Oryzias latipes trichohyalin (LOC101166347), transcript variant X4, mRNA.

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CloneID	OJa113160
Clone ID Related Accession (Same CDS sequence)	XM_023950731.1
Accession Version	XM_023950731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	trichohyalin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG GGTTGCTGAT TGGCTGGCAG CAGCAGAAGG AGGAGCTCGA GCGGGAGGTA 
TGCCGTCTGC AGGAGGAGCT GGCAGAGAGT CGAGCGGAGC GGGAGGAGTT GGAGTCCAGA 
ACCCGAGCTC TGCAGGACAG GGTGAGGCTC CGTCAGACCA TACATCCGTC ACTTGGCCTG 
TCACTTGAGG AGGAGGAGGA GCAGAGGAGG TGGAAGAAGC AGCTTAGGGA GGGCAGGGAG 
AGAGAGGCCA GGCAGGCACT GCTGATACAT CGGCTGCAGA ACAAGGTTCT GGAGTACAGA 
GATCAGTGCC AGCATTTGGA CCTGCAGTTG CAAGATGAAC GGAGGAAGAC GCTCCACATG 
GAGGGGGTCC GGGATGAACA TAGCAACTCT CTGGAAAGCG CCCTCATCAG GCTGGAGGAG 
GAGCAGCACA GGTCCGTAAG TCTGGCTGAC ACCAACTCTC TTCTGCGGGA GCAGCTCAGC 
CAATCTGAGA AGTTCAACCA GGAGCTTAAG GAGGAACTCC ACAGACTGAC AGCTGATTGG 
ATCAGATCCA CGGAGAAGGC GGAGCAAAGA GAAACTGATT GGCAGAGAGA GAAAGAGGAT 
CAGCGGGGTC ACGTGGGTCA GCAGCAGAAC AACTGGCTGT CAGTTTGGAG ATCCGTGGTG 
GCTCTGAGGA GACAATGTCA CAGCGTGAAA ACAGCTGCCG ACAGAGACCT GTGGGAGCTC 
AGGGCCGAGT TTTCCCGTCT CTCCTCAACA CTCCTCTCCA GCTGTGACTC TGTCTGCTCC 
ACCCAGAGCC TGGCGGCTCT TCACAAGCCT TTGTCTTCAA CTTTGCTGCC TCTTCCTTCC 
CCACCATCCA GTTCTCCTCC TCTATCCTCA ACACTGATCC TCCCTCCTCC TCCTCCAGAC 
TCCTGCTCCA CTCAGGGATC CTTCTCCATG GGGGAGCTAG AACTCAAAGA GGAGGAGGGA 
GAAGGTTCTG AGCCTAAGCT CTATGAAAAG ACTGAAGCTT TGCAGCAGAG GACTGTGGAG 
CTTGCTCGCT CACTGCAGGA TGAGAGAGAG GAGAAGGAGA GGGAGGTGGA AAGACATCAA 
GAAACTGCAA GGAGACTCCA GTCTGTCAGT CAGGCTGTGA TCAGACTGGC CAGAGTCGTC 
AGCAGCAGAA GTCGGCCAGC GAGCACCTCC CCAGAAGACG TCCTGCGGGC GGATTTGTCC 
TCCATGCTGG CTGTGCTCTC ACAGGCGGAA AGCGTCCTGC TGAGGAGGAG CGAGGAGCTG 
CAGGGGGCGG AGCTAAACCT GCGACGTCTC GGTGAGGTGA GAACCAGCTT GCAGCTGCAG 
GTGAAGCAGC TTCAGGACGA CCACCAGCAG CTTCTGGCAC GCTCACAACA CACACAGCAG 
GAGCTCACGC ACACACAGAG CGTCCTCAGC AGCGAGAAAA AGGTCTCCAG CTCCCTCCGC 
TTGCAGCTGG AGGAGGTGCA AAGAAGAGAG GAGGAGGTGA AGAGAGAGAA CCACAGACTG 
AGGAGAGAGA GAGACAGAGA GGAAGAGAAG AATCTGCAAC TGGAAACAGA CTCACATCGA 
CGGGTGGAGG CGGAGCTCCT AGAAAGTGTC CAGCTGACGG AGAGAGAACA TCTGCACCAG 
ATGGAGCTCC TCAGTCTGAA GGGGGCGCTG GAGAGAGAAC AGCTGGACAG GCAGAAAGCA 
GAGGAAGAGG CAGCAGGCTC TAGGGATGCC CTACAGCAGT GCAGGGGAAG CATACTCCGC 
CTCTCATCCT CAGAAAGCCT GCTGAGGCGG GATCTGGAGG AGAGTCGAGA TGCTTTGAAG 
AAGGTTTCTG CTCTTAACTC TGCTCTGGCC TCAGACAAGA GAGCACTGAA CCAGCAGCTG 
CTGCAGCTGG AAGGCAAACT GTCAGAGAGC CAATCGCAGC TGCAAGCTGT GAGTTCAGAG 
TTCACCTGTC TGCAGAGAGA CGTCAGACCT CTCAGACTGC AGAGGGATCA GGATGCCGAC 
GTCATCCGTC AGCTGAAAGA GCAGCAGGTG GAGGCTGAGA AAAACCTGGA GAGAAAGGAG 
AGAGAGCTTC AGCTATTGGA GGAGGAGAGA ACAAGTGGTG AACAACAGCT GGAGGAGCTT 
CGCTCTCATC ACGTCTCGCT GTGCCAGGAG CTGAAGGAGT TGCAGGAGGA GTTGCAACAG 
GCGAAACAGG AGGTTAGGAT TCTGAATTGG CAGCAGGAGG AGCAGCAAAG GGAGAGCAGG 
AGCCGGCAGG AGGAGCTCCA GAATGAGCAT AGACAGCGGG CGCAGCTGGA GGAGGAGCTG 
CATGAGCTCA GGCCTCTGTC AGTGTCCCTG CAGCTGCAGC TCAGCCAGCA GCAGCAGCTC 
CTCTCCCAGG TCGAGGTGGA CCGCTGTCAG CTGAGCACTC GCCTCCATGC GCTGCAGGAG 
GCCAAGCTCA CCTTACAGGG TGAAATCAAA TGTCTGAGAG GGGAGCTGCA GAGAGAGACA 
GCCAGCAGAG AGAGAAGTGA GGGCGAGAAG GAGGTGCTGC AGGAAGACCT GCAGAGACTG 
ACGGAAGAGG TGCAGGAGCT GAGGAGAAGA AAGGCAGAAA AGGAAGAGGA GAAAAAGATG 
TGCCAAAGAG AACGAGAGAC ACTGAGGGAG GAATTAAGGC TGAGAGACGG GGAGCTGGAA 
GCCCTGAAGA CACGCACTGA AAGGATGGAA GAGGAGCTGG AAGAAAAGGG ACAACAGGTG 
GAGAGACTGA AGGAGGAGGT GACACAACGA GCTACCCAAG TCAGCTTCCT AAATGAGAGA 
ATAGTTGGCT TGGAAGAGGA AAAGAAGATT CTGCTGGAGG AGGCAAAGAG TACTGAGGTC 
AGACTGCTGG AGGAGGAGGC GAAAAGGAAA GACGAGGAGG TGCAAAGAGA TGGGGAGACT 
GAAGCTCTCT GTGACAGAAT AGAGAAATTA GAAAGAGAGA AGGAAGAAAT AGATAAGGAG 
GTGCACCAAA TGAAGAGAAA AGCACAGGAG CAAGAGGAGA GATGGAGGTC AAATGATGAG 
GAGAGCCAGA ATGAGATAAA CAGACTAAGG GGAGAAAACT CTTCCCTCCA GGAGCACAGA 
AAGTCAGAGA AGCGACAAAT AGAGAAACTG CAAAATGAAA AAATGGAGGT GCTGGAGAGG 
CTACAAGAGA AAGTTGGAGA GGTGGAACTG ATGAAGGTAA AACTAAGTGT GGCTCAGGAG 
GAGAGTGAGG AGCTGAGGGA GGAGGTGCAG AGGAGGGAGC GCAGCCTGGA GAAACAGATA 
TGTGCAGTAC GAGAAAGGGA GGAGGAAATG GAGAACCTGA GGGGGCGGCT GAGGTTGGTG 
AAGGAGCGGG AGGCGGAAGC AGAGAGAGAG CTCCAGGAAA CTTCTGACAA ACTGAAAAAA 
AAAGAGGCGA GGGAGGAACA CCTGGAGGTG CTGCTTAGAG AAAACCGGGA ACTTTTGAAG 
CAGGAGGAAA AAGGACGAGA AGAAAGAGAA GACAGGATCT GCTCCTTGAT CAAACAATTG 
CAGGAGGCCC AGGCTGTTAA CCTGGGAGCA AAAAGAAGAA GCACAGAAAG GCAGAAGGCC 
CATGACAGAC TGCAGGAGGA GGTGAAGGAC TGGCAGGAGC AAGCAGAACA AAGTGTGAAG 
GAGGTGGCAA AACTCAAAGA AATCCTGAAG GGGAGAAATG AGGAGGTGAA CCAGCTGAAA 
GCAATGCTTG AAGAAAAAGA AGAGGAGTGG AAAGAGCAGG AGGCTTTGAG TAAAACAGAG 
AAAGAAAGGA GGAGAAGGCT GGAAAACAAG CTGGTCGAGG TGGAGGAAGA GAACACCAGG 
TTAGAGAAGG AGGTGAGACT GATGCAGGCA GTTGGAGATA AAGATAGGGA GGCTCTGGCA 
AAGGCTGGAG AGGAGTTAAA AACATTGAAG GACAACCTGG TAAAAAGTGA AAACAAAGAG 
AGGGAGCTGG GAGAAACTGT GAGAAGAATG GAAGAGGAGA AGATGATGCT GAGTAGCAAT 
TTGAGAGAGA TGGAGGACGA GATCAAAGCA GAGAAGGAGC AGTTGTGGAG AGTAAGGAAA 
GAGAGGAGCA GTCTGGAGGA CAAACTAAAA GAAAAAGAGA GAGAAATAGA TGAGTGCAGG 
GATTTGCTTA GAAGTCAGGA GGAAAAACTT GAGGAGGTGA AAAAGATGGA TAGAAAAACA 
TTGGAGAGTG AAAGAAATCG GTATCTACTG TTGGAGGAAA AAGGGAAGAC CAGGGTTAAA 
AAGCTTGAGG AGGAGAAGGA GGACCTTTTA CTGCAGTTAA TGAAAAGGGA AAAAGAAGTG 
ACAGAACTCC AAGAGGAGGT GCAGAGGCAG AGGATCACTG CAAGCAAGGA GCTCAATGAA 
AAGAAAGAGG AGGCATTTAA TGTACAAAGG GAATGTGAGG AGGCGCAGGA AAAGCTAAAA 
TCATTGAAAA GGGAAATGAT TTATCTCCAA GAGGAGGTGA AACAAAAGCA AGAAGAGAAT 
GAGGAGGTGC AGGAAAAACT GCAATCATTG AAGAGGGAAA TGATTTATCT CCAAGAGGAG 
GTGAAACAAA AGCAAGAAGA GAATGAGGAG GTGCAGGAAA AACTGCAATC ATTGAAGAGG 
GAAATGATTT ATCTCCAGGA GGAGGTGAAA CAAAAGCAAG AAGACAATGA GGAGGTGCAG 
GAAAAACTCC AATCATTAAA AAGCGAAATG ATTTATCTCC AAGAGGAGGT GAAACAAAAG 
CAAAAAGAGA ATGAGGAGAC ACAGGAGAAA ATGACCGTGT TAAAGGAGGT GCACCAGAAA 
CATTTAAAGA AAGAGGAAGA GTGGAGAACT AACGATATGA TGAGCAGAAA TGACACCCAG 
GAGCTACAGA GGCAGAAAGA TGAGGCTCAA GAGGAGCTGC AGAGGAGCAG AAAGGAGATC 
TACATACTTA GAGAGGAGCT AGAAAAGGAA CAAAGAGAAA AAAAGGACCT TCAAGATGAG 
ATGAAAAAGA GGAGTGAAGA GGCTGGATTG CTAAGACAGA AAATAGAGGA GGAGTTCAGC 
AGCACCAAGA AGAGGGAGGA GACAGAAAAA CAAACAAGAG AGAAGACTGA GATAGAGTTG 
GCCGCTCTAA AGGAGGAGCT GCAAAGGGAA CACAGGGAGA AATTGGAGAC AGTGGAGGAG 
CTGAAGAAGA GGAGAGAGGA TGTCACTGTG CTTGAAGGAG AGGTGAAGGA GGAGTGCATG 
AAGAAGGAGG AGGTGCAGGA AGATCTAAGA AGAGTGCAGC ACAGCTTGGA GCAGATGTCT 
CACAGTCTGA GCTCCCTTCA GAGTCAGGTG TCAGAGCTGA GTATCAGCAA GGAGCGAGTA 
GAGGTGGAGA AGCAGCAGAT GAGAGAAGGC CTGCAGGTGG CGCTCAAGGA GATGGACTTG 
CTGAAAGTTC TCCTCCAGGA AAGCCATGCA GAAGGAGAAC TGCTGAGGAG CACACTGGAG 
GAGAAGAAGA AGGAGATGGG GAGGGTTCGA CAGGAGAACC TGCAGATGGA AGAGAGGCAA 
GAGGAGCTGG AGGAGCTGAG AGCCAGGGTC AAAGCCCTGG AGAGAAGAAG GACGGAGATG 
GCAGAGGAGC TAGATAGGGC AGATGAGAGG AGGAGGGAGG CTGAGGAGTG GTGGAGGAGC 
AAAGTGAAGG AAGTAGAACG GCAGAAGGGG ACGATGCAAA GCACTCTTTA CACAGAAATA 
CAGACGCAAG GGGAGCTACT GAAGGAGTCG AGGTCCAAGC TTCAGGACAG TTTGAGGGAG 
CTGGAGGAGG CGAGAATGGA GCTTAGCCGG AACAGAGCCT TGTTGGAGGA GCAGAAAACT 
TTCACGATTG CTGACAGAGA GGATTCGGGG GAGCAGAGGA GCAGAGGCAG CCAAGAGGAA 
AGAGGGGAGG TGAAGCAGGA GGAGATCAGG AGGCAGCTCC AGCAGAGGGA GGCTCAGGTG 
GACAGTTTGA CTCTCAGAGT CCAGGAGCTG CAGGAAGACA GAAATCAGCT CCGATTGGCT 
CTGGAACAAA CTGAGGCGCA GCTGAAAAAC GTCGCCACCC AGACTGTAGG GGGCAGACTG 
GAGGTCCTGC AGAGACTGGT GGCTGAACTG GAGCTCCAAC GGACACGACT GCAGAAGAAG 
AACTTGAATC TAGAAAAGCA AAAAGACAGA CTGAAGAAAG ACAGAGACTT TCTGAGAGAC 
ACCCTGAGAC AGGTGGAGCT GGAGCGTGGG AGGCTCAGCC AGCAGCTGAC AGACAGCAGA 
GGATCTCAGG TGGAACAGGT GTGCGCTAAT GCCACAGAAG AAGATCGTCT GCGCAGCAAA 
GTGAAGGAGC TGGAGGAGCA GGTTAGTCAG CTTCGTCTTT CTTTAGCTAT GGACCAGCAG 
CAGAAGGCGG AGTTTATCCA GCAGTCATCC AGAAACAGCC AATGGCTGCT CTCACTAAGA 
CGGGACCTCA ATGATTCGCT GTCTGCTGTG ACCCGTCTAC CAATCCCGTC TGTCCTGGAG 
TCTGAGACAC AGAGGTTGGA CCGCAGCCTG AGGGAGGAGG AGCTTAGGAT TTCCTTGAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806499.1
CDS	297..6779
Translation	MDSGLLIGWQQQKEELEREVCRLQEELAESRAEREELESRTRALQDRVRLRQTIHPSLGLSLEEEEEQRRWKKQLREGREREARQALLIHRLQNKVLEYRDQCQHLDLQLQDERRKTLHMEGVRDEHSNSLESALIRLEEEQHRSVSLADTNSLLREQLSQSEKFNQELKEELHRLTADWIRSTEKAEQRETDWQREKEDQRGHVGQQQNNWLSVWRSVVALRRQCHSVKTAADRDLWELRAEFSRLSSTLLSSCDSVCSTQSLAALHKPLSSTLLPLPSPPSSSPPLSSTLILPPPPPDSCSTQGSFSMGELELKEEEGEGSEPKLYEKTEALQQRTVELARSLQDEREEKEREVERHQETARRLQSVSQAVIRLARVVSSRSRPASTSPEDVLRADLSSMLAVLSQAESVLLRRSEELQGAELNLRRLGEVRTSLQLQVKQLQDDHQQLLARSQHTQQELTHTQSVLSSEKKVSSSLRLQLEEVQRREEEVKRENHRLRRERDREEEKNLQLETDSHRRVEAELLESVQLTEREHLHQMELLSLKGALEREQLDRQKAEEEAAGSRDALQQCRGSILRLSSSESLLRRDLEESRDALKKVSALNSALASDKRALNQQLLQLEGKLSESQSQLQAVSSEFTCLQRDVRPLRLQRDQDADVIRQLKEQQVEAEKNLERKERELQLLEEERTSGEQQLEELRSHHVSLCQELKELQEELQQAKQEVRILNWQQEEQQRESRSRQEELQNEHRQRAQLEEELHELRPLSVSLQLQLSQQQQLLSQVEVDRCQLSTRLHALQEAKLTLQGEIKCLRGELQRETASRERSEGEKEVLQEDLQRLTEEVQELRRRKAEKEEEKKMCQRERETLREELRLRDGELEALKTRTERMEEELEEKGQQVERLKEEVTQRATQVSFLNERIVGLEEEKKILLEEAKSTEVRLLEEEAKRKDEEVQRDGETEALCDRIEKLEREKEEIDKEVHQMKRKAQEQEERWRSNDEESQNEINRLRGENSSLQEHRKSEKRQIEKLQNEKMEVLERLQEKVGEVELMKVKLSVAQEESEELREEVQRRERSLEKQICAVREREEEMENLRGRLRLVKEREAEAERELQETSDKLKKKEAREEHLEVLLRENRELLKQEEKGREEREDRICSLIKQLQEAQAVNLGAKRRSTERQKAHDRLQEEVKDWQEQAEQSVKEVAKLKEILKGRNEEVNQLKAMLEEKEEEWKEQEALSKTEKERRRRLENKLVEVEEENTRLEKEVRLMQAVGDKDREALAKAGEELKTLKDNLVKSENKERELGETVRRMEEEKMMLSSNLREMEDEIKAEKEQLWRVRKERSSLEDKLKEKEREIDECRDLLRSQEEKLEEVKKMDRKTLESERNRYLLLEEKGKTRVKKLEEEKEDLLLQLMKREKEVTELQEEVQRQRITASKELNEKKEEAFNVQRECEEAQEKLKSLKREMIYLQEEVKQKQEENEEVQEKLQSLKREMIYLQEEVKQKQEENEEVQEKLQSLKREMIYLQEEVKQKQEDNEEVQEKLQSLKSEMIYLQEEVKQKQKENEETQEKMTVLKEVHQKHLKKEEEWRTNDMMSRNDTQELQRQKDEAQEELQRSRKEIYILREELEKEQREKKDLQDEMKKRSEEAGLLRQKIEEEFSSTKKREETEKQTREKTEIELAALKEELQREHREKLETVEELKKRREDVTVLEGEVKEECMKKEEVQEDLRRVQHSLEQMSHSLSSLQSQVSELSISKERVEVEKQQMREGLQVALKEMDLLKVLLQESHAEGELLRSTLEEKKKEMGRVRQENLQMEERQEELEELRARVKALERRRTEMAEELDRADERRREAEEWWRSKVKEVERQKGTMQSTLYTEIQTQGELLKESRSKLQDSLRELEEARMELSRNRALLEEQKTFTIADREDSGEQRSRGSQEERGEVKQEEIRRQLQQREAQVDSLTLRVQELQEDRNQLRLALEQTEAQLKNVATQTVGGRLEVLQRLVAELELQRTRLQKKNLNLEKQKDRLKKDRDFLRDTLRQVELERGRLSQQLTDSRGSQVEQVCANATEEDRLRSKVKELEEQVSQLRLSLAMDQQQKAEFIQQSSRNSQWLLSLRRDLNDSLSAVTRLPIPSVLESETQRLDRSLREEELRISLS

Target ORF information:

RefSeq Version	XM_023950731.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes trichohyalin (LOC101166347), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950731.1

ORF Insert Sequence:

ATGGATTCTG GGTTGCTGAT TGGCTGGCAG CAGCAGAAGG AGGAGCTCGA GCGGGAGGTA 
TGCCGTCTGC AGGAGGAGCT GGCAGAGAGT CGAGCGGAGC GGGAGGAGTT GGAGTCCAGA 
ACCCGAGCTC TGCAGGACAG GGTGAGGCTC CGTCAGACCA TACATCCGTC ACTTGGCCTG 
TCACTTGAGG AGGAGGAGGA GCAGAGGAGG TGGAAGAAGC AGCTTAGGGA GGGCAGGGAG 
AGAGAGGCCA GGCAGGCACT GCTGATACAT CGGCTGCAGA ACAAGGTTCT GGAGTACAGA 
GATCAGTGCC AGCATTTGGA CCTGCAGTTG CAAGATGAAC GGAGGAAGAC GCTCCACATG 
GAGGGGGTCC GGGATGAACA TAGCAACTCT CTGGAAAGCG CCCTCATCAG GCTGGAGGAG 
GAGCAGCACA GGTCCGTAAG TCTGGCTGAC ACCAACTCTC TTCTGCGGGA GCAGCTCAGC 
CAATCTGAGA AGTTCAACCA GGAGCTTAAG GAGGAACTCC ACAGACTGAC AGCTGATTGG 
ATCAGATCCA CGGAGAAGGC GGAGCAAAGA GAAACTGATT GGCAGAGAGA GAAAGAGGAT 
CAGCGGGGTC ACGTGGGTCA GCAGCAGAAC AACTGGCTGT CAGTTTGGAG ATCCGTGGTG 
GCTCTGAGGA GACAATGTCA CAGCGTGAAA ACAGCTGCCG ACAGAGACCT GTGGGAGCTC 
AGGGCCGAGT TTTCCCGTCT CTCCTCAACA CTCCTCTCCA GCTGTGACTC TGTCTGCTCC 
ACCCAGAGCC TGGCGGCTCT TCACAAGCCT TTGTCTTCAA CTTTGCTGCC TCTTCCTTCC 
CCACCATCCA GTTCTCCTCC TCTATCCTCA ACACTGATCC TCCCTCCTCC TCCTCCAGAC 
TCCTGCTCCA CTCAGGGATC CTTCTCCATG GGGGAGCTAG AACTCAAAGA GGAGGAGGGA 
GAAGGTTCTG AGCCTAAGCT CTATGAAAAG ACTGAAGCTT TGCAGCAGAG GACTGTGGAG 
CTTGCTCGCT CACTGCAGGA TGAGAGAGAG GAGAAGGAGA GGGAGGTGGA AAGACATCAA 
GAAACTGCAA GGAGACTCCA GTCTGTCAGT CAGGCTGTGA TCAGACTGGC CAGAGTCGTC 
AGCAGCAGAA GTCGGCCAGC GAGCACCTCC CCAGAAGACG TCCTGCGGGC GGATTTGTCC 
TCCATGCTGG CTGTGCTCTC ACAGGCGGAA AGCGTCCTGC TGAGGAGGAG CGAGGAGCTG 
CAGGGGGCGG AGCTAAACCT GCGACGTCTC GGTGAGGTGA GAACCAGCTT GCAGCTGCAG 
GTGAAGCAGC TTCAGGACGA CCACCAGCAG CTTCTGGCAC GCTCACAACA CACACAGCAG 
GAGCTCACGC ACACACAGAG CGTCCTCAGC AGCGAGAAAA AGGTCTCCAG CTCCCTCCGC 
TTGCAGCTGG AGGAGGTGCA AAGAAGAGAG GAGGAGGTGA AGAGAGAGAA CCACAGACTG 
AGGAGAGAGA GAGACAGAGA GGAAGAGAAG AATCTGCAAC TGGAAACAGA CTCACATCGA 
CGGGTGGAGG CGGAGCTCCT AGAAAGTGTC CAGCTGACGG AGAGAGAACA TCTGCACCAG 
ATGGAGCTCC TCAGTCTGAA GGGGGCGCTG GAGAGAGAAC AGCTGGACAG GCAGAAAGCA 
GAGGAAGAGG CAGCAGGCTC TAGGGATGCC CTACAGCAGT GCAGGGGAAG CATACTCCGC 
CTCTCATCCT CAGAAAGCCT GCTGAGGCGG GATCTGGAGG AGAGTCGAGA TGCTTTGAAG 
AAGGTTTCTG CTCTTAACTC TGCTCTGGCC TCAGACAAGA GAGCACTGAA CCAGCAGCTG 
CTGCAGCTGG AAGGCAAACT GTCAGAGAGC CAATCGCAGC TGCAAGCTGT GAGTTCAGAG 
TTCACCTGTC TGCAGAGAGA CGTCAGACCT CTCAGACTGC AGAGGGATCA GGATGCCGAC 
GTCATCCGTC AGCTGAAAGA GCAGCAGGTG GAGGCTGAGA AAAACCTGGA GAGAAAGGAG 
AGAGAGCTTC AGCTATTGGA GGAGGAGAGA ACAAGTGGTG AACAACAGCT GGAGGAGCTT 
CGCTCTCATC ACGTCTCGCT GTGCCAGGAG CTGAAGGAGT TGCAGGAGGA GTTGCAACAG 
GCGAAACAGG AGGTTAGGAT TCTGAATTGG CAGCAGGAGG AGCAGCAAAG GGAGAGCAGG 
AGCCGGCAGG AGGAGCTCCA GAATGAGCAT AGACAGCGGG CGCAGCTGGA GGAGGAGCTG 
CATGAGCTCA GGCCTCTGTC AGTGTCCCTG CAGCTGCAGC TCAGCCAGCA GCAGCAGCTC 
CTCTCCCAGG TCGAGGTGGA CCGCTGTCAG CTGAGCACTC GCCTCCATGC GCTGCAGGAG 
GCCAAGCTCA CCTTACAGGG TGAAATCAAA TGTCTGAGAG GGGAGCTGCA GAGAGAGACA 
GCCAGCAGAG AGAGAAGTGA GGGCGAGAAG GAGGTGCTGC AGGAAGACCT GCAGAGACTG 
ACGGAAGAGG TGCAGGAGCT GAGGAGAAGA AAGGCAGAAA AGGAAGAGGA GAAAAAGATG 
TGCCAAAGAG AACGAGAGAC ACTGAGGGAG GAATTAAGGC TGAGAGACGG GGAGCTGGAA 
GCCCTGAAGA CACGCACTGA AAGGATGGAA GAGGAGCTGG AAGAAAAGGG ACAACAGGTG 
GAGAGACTGA AGGAGGAGGT GACACAACGA GCTACCCAAG TCAGCTTCCT AAATGAGAGA 
ATAGTTGGCT TGGAAGAGGA AAAGAAGATT CTGCTGGAGG AGGCAAAGAG TACTGAGGTC 
AGACTGCTGG AGGAGGAGGC GAAAAGGAAA GACGAGGAGG TGCAAAGAGA TGGGGAGACT 
GAAGCTCTCT GTGACAGAAT AGAGAAATTA GAAAGAGAGA AGGAAGAAAT AGATAAGGAG 
GTGCACCAAA TGAAGAGAAA AGCACAGGAG CAAGAGGAGA GATGGAGGTC AAATGATGAG 
GAGAGCCAGA ATGAGATAAA CAGACTAAGG GGAGAAAACT CTTCCCTCCA GGAGCACAGA 
AAGTCAGAGA AGCGACAAAT AGAGAAACTG CAAAATGAAA AAATGGAGGT GCTGGAGAGG 
CTACAAGAGA AAGTTGGAGA GGTGGAACTG ATGAAGGTAA AACTAAGTGT GGCTCAGGAG 
GAGAGTGAGG AGCTGAGGGA GGAGGTGCAG AGGAGGGAGC GCAGCCTGGA GAAACAGATA 
TGTGCAGTAC GAGAAAGGGA GGAGGAAATG GAGAACCTGA GGGGGCGGCT GAGGTTGGTG 
AAGGAGCGGG AGGCGGAAGC AGAGAGAGAG CTCCAGGAAA CTTCTGACAA ACTGAAAAAA 
AAAGAGGCGA GGGAGGAACA CCTGGAGGTG CTGCTTAGAG AAAACCGGGA ACTTTTGAAG 
CAGGAGGAAA AAGGACGAGA AGAAAGAGAA GACAGGATCT GCTCCTTGAT CAAACAATTG 
CAGGAGGCCC AGGCTGTTAA CCTGGGAGCA AAAAGAAGAA GCACAGAAAG GCAGAAGGCC 
CATGACAGAC TGCAGGAGGA GGTGAAGGAC TGGCAGGAGC AAGCAGAACA AAGTGTGAAG 
GAGGTGGCAA AACTCAAAGA AATCCTGAAG GGGAGAAATG AGGAGGTGAA CCAGCTGAAA 
GCAATGCTTG AAGAAAAAGA AGAGGAGTGG AAAGAGCAGG AGGCTTTGAG TAAAACAGAG 
AAAGAAAGGA GGAGAAGGCT GGAAAACAAG CTGGTCGAGG TGGAGGAAGA GAACACCAGG 
TTAGAGAAGG AGGTGAGACT GATGCAGGCA GTTGGAGATA AAGATAGGGA GGCTCTGGCA 
AAGGCTGGAG AGGAGTTAAA AACATTGAAG GACAACCTGG TAAAAAGTGA AAACAAAGAG 
AGGGAGCTGG GAGAAACTGT GAGAAGAATG GAAGAGGAGA AGATGATGCT GAGTAGCAAT 
TTGAGAGAGA TGGAGGACGA GATCAAAGCA GAGAAGGAGC AGTTGTGGAG AGTAAGGAAA 
GAGAGGAGCA GTCTGGAGGA CAAACTAAAA GAAAAAGAGA GAGAAATAGA TGAGTGCAGG 
GATTTGCTTA GAAGTCAGGA GGAAAAACTT GAGGAGGTGA AAAAGATGGA TAGAAAAACA 
TTGGAGAGTG AAAGAAATCG GTATCTACTG TTGGAGGAAA AAGGGAAGAC CAGGGTTAAA 
AAGCTTGAGG AGGAGAAGGA GGACCTTTTA CTGCAGTTAA TGAAAAGGGA AAAAGAAGTG 
ACAGAACTCC AAGAGGAGGT GCAGAGGCAG AGGATCACTG CAAGCAAGGA GCTCAATGAA 
AAGAAAGAGG AGGCATTTAA TGTACAAAGG GAATGTGAGG AGGCGCAGGA AAAGCTAAAA 
TCATTGAAAA GGGAAATGAT TTATCTCCAA GAGGAGGTGA AACAAAAGCA AGAAGAGAAT 
GAGGAGGTGC AGGAAAAACT GCAATCATTG AAGAGGGAAA TGATTTATCT CCAAGAGGAG 
GTGAAACAAA AGCAAGAAGA GAATGAGGAG GTGCAGGAAA AACTGCAATC ATTGAAGAGG 
GAAATGATTT ATCTCCAGGA GGAGGTGAAA CAAAAGCAAG AAGACAATGA GGAGGTGCAG 
GAAAAACTCC AATCATTAAA AAGCGAAATG ATTTATCTCC AAGAGGAGGT GAAACAAAAG 
CAAAAAGAGA ATGAGGAGAC ACAGGAGAAA ATGACCGTGT TAAAGGAGGT GCACCAGAAA 
CATTTAAAGA AAGAGGAAGA GTGGAGAACT AACGATATGA TGAGCAGAAA TGACACCCAG 
GAGCTACAGA GGCAGAAAGA TGAGGCTCAA GAGGAGCTGC AGAGGAGCAG AAAGGAGATC 
TACATACTTA GAGAGGAGCT AGAAAAGGAA CAAAGAGAAA AAAAGGACCT TCAAGATGAG 
ATGAAAAAGA GGAGTGAAGA GGCTGGATTG CTAAGACAGA AAATAGAGGA GGAGTTCAGC 
AGCACCAAGA AGAGGGAGGA GACAGAAAAA CAAACAAGAG AGAAGACTGA GATAGAGTTG 
GCCGCTCTAA AGGAGGAGCT GCAAAGGGAA CACAGGGAGA AATTGGAGAC AGTGGAGGAG 
CTGAAGAAGA GGAGAGAGGA TGTCACTGTG CTTGAAGGAG AGGTGAAGGA GGAGTGCATG 
AAGAAGGAGG AGGTGCAGGA AGATCTAAGA AGAGTGCAGC ACAGCTTGGA GCAGATGTCT 
CACAGTCTGA GCTCCCTTCA GAGTCAGGTG TCAGAGCTGA GTATCAGCAA GGAGCGAGTA 
GAGGTGGAGA AGCAGCAGAT GAGAGAAGGC CTGCAGGTGG CGCTCAAGGA GATGGACTTG 
CTGAAAGTTC TCCTCCAGGA AAGCCATGCA GAAGGAGAAC TGCTGAGGAG CACACTGGAG 
GAGAAGAAGA AGGAGATGGG GAGGGTTCGA CAGGAGAACC TGCAGATGGA AGAGAGGCAA 
GAGGAGCTGG AGGAGCTGAG AGCCAGGGTC AAAGCCCTGG AGAGAAGAAG GACGGAGATG 
GCAGAGGAGC TAGATAGGGC AGATGAGAGG AGGAGGGAGG CTGAGGAGTG GTGGAGGAGC 
AAAGTGAAGG AAGTAGAACG GCAGAAGGGG ACGATGCAAA GCACTCTTTA CACAGAAATA 
CAGACGCAAG GGGAGCTACT GAAGGAGTCG AGGTCCAAGC TTCAGGACAG TTTGAGGGAG 
CTGGAGGAGG CGAGAATGGA GCTTAGCCGG AACAGAGCCT TGTTGGAGGA GCAGAAAACT 
TTCACGATTG CTGACAGAGA GGATTCGGGG GAGCAGAGGA GCAGAGGCAG CCAAGAGGAA 
AGAGGGGAGG TGAAGCAGGA GGAGATCAGG AGGCAGCTCC AGCAGAGGGA GGCTCAGGTG 
GACAGTTTGA CTCTCAGAGT CCAGGAGCTG CAGGAAGACA GAAATCAGCT CCGATTGGCT 
CTGGAACAAA CTGAGGCGCA GCTGAAAAAC GTCGCCACCC AGACTGTAGG GGGCAGACTG 
GAGGTCCTGC AGAGACTGGT GGCTGAACTG GAGCTCCAAC GGACACGACT GCAGAAGAAG 
AACTTGAATC TAGAAAAGCA AAAAGACAGA CTGAAGAAAG ACAGAGACTT TCTGAGAGAC 
ACCCTGAGAC AGGTGGAGCT GGAGCGTGGG AGGCTCAGCC AGCAGCTGAC AGACAGCAGA 
GGATCTCAGG TGGAACAGGT GTGCGCTAAT GCCACAGAAG AAGATCGTCT GCGCAGCAAA 
GTGAAGGAGC TGGAGGAGCA GGTTAGTCAG CTTCGTCTTT CTTTAGCTAT GGACCAGCAG 
CAGAAGGCGG AGTTTATCCA GCAGTCATCC AGAAACAGCC AATGGCTGCT CTCACTAAGA 
CGGGACCTCA ATGATTCGCT GTCTGCTGTG ACCCGTCTAC CAATCCCGTC TGTCCTGGAG 
TCTGAGACAC AGAGGTTGGA CCGCAGCCTG AGGGAGGAGG AGCTTAGGAT TTCCTTGAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101166347 ( XM_020713302.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020568961.1 Oryzias latipes trichohyalin-like (LOC101166347), mRNA.

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CloneID	OJa75542
Clone ID Related Accession (Same CDS sequence)	XM_020713302.1
Accession Version	XM_020713302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	trichohyalin-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 31% of CDS bases ##RefSeq-Attributes-END##

ATGGATTCTG GGTTGCTGAT TGGCTGGCAG CAGCAGAAGG AGGAGCTCGA GCGGGAGGTA 
TGCCGTCTGC AGGAGGAGCT GGCAGAGAGT CGAGCGGAGC GGGAGGAGTT GGAGTCCAGA 
ACCCGAGCTC TGCAGGACAG GGTGAGGCTC CGTCAGACCA TACATCCGTC ACTTGGCCTG 
TCACTTGAGG AGGAGGAGGA GCAGAGGAGG TGGAAGAAGC AGCTTAGGGA GGGCAGGGAG 
AGAGAGGCCA GGCAGGCACT GCTGATACAT CGGCTGCAGA ACAAGGTTCT GGAGTACAGA 
GATCAGTGCC AGCATTTGGA CCTGCAGTTG CAAGATGAAC GGAGGAAGAC GCTCCACATG 
GAGGGGGTCC GGGATGAACA TAGCAACTCT CTGGAAAGCG CCCTCATCAG GCTGGAGGAG 
GAGCAGCACA GGTCCGTAAG TCTGGCTGAC ACCAACTCTC TTCTGCGGGA GCAGCTCAGC 
CAATCTGAGA AGTTCAACCA GGAGCTTAAG GAGGAACTCC ACAGACTGAC AGCTGATTGG 
ATCAGATCCA CGGAGAAGGC GGAGCAAAGA GAAACTGATT GGCAGAGAGA GAAAGAGGAT 
CAGCGGGGTC ACGTGGGTCA GCAGCAGAAC AACTGGCTGT CAGTTTGGAG ATCCGTGGTG 
GCTCTGAGGA GACAATGTCA CAGCGTGAAA ACAGCTGCCG ACAGAGACCT GTGGGAGCTC 
AGGGCCGAGT TTTCCCGTCT CTCCTCAACA CTCCTCTCCA GCTGTGACTC TGTCTGCTCC 
ACCCAGAGCC TGGCGGCTCT TCACAAGCCT TTGTCTTCAA CTTTGCTGCC TCTTCCTTCC 
CCACCATCCA GTTCTCCTCC TCTATCCTCA ACACTGATCC TCCCTCCTCC TCCTCCAGAC 
TCCTGCTCCA CTCAGGGATC CTTCTCCATG GGGGAGCTAG AACTCAAAGA GGAGGAGGGA 
GAAGGTTCTG AGCCTAAGCT CTATGAAAAG ACTGAAGCTT TGCAGCAGAG GACTGTGGAG 
CTTGCTCGCT CACTGCAGGA TGAGAGAGAG GAGAAGGAGA GGGAGGTGGA AAGACATCAA 
GAAACTGCAA GGAGACTCCA GTCTGTCAGT CAGGCTGTGA TCAGACTGGC CAGAGTCGTC 
AGCAGCAGAA GTCGGCCAGC GAGCACCTCC CCAGAAGACG TCCTGCGGGC GGATTTGTCC 
TCCATGCTGG CTGTGCTCTC ACAGGCGGAA AGCGTCCTGC TGAGGAGGAG CGAGGAGCTG 
CAGGGGGCGG AGCTAAACCT GCGACGTCTC GGTGAGGTGA GAACCAGCTT GCAGCTGCAG 
GTGAAGCAGC TTCAGGACGA CCACCAGCAG CTTCTGGCAC GCTCACAACA CACACAGCAG 
GAGCTCACGC ACACACAGAG CGTCCTCAGC AGCGAGAAAA AGGTCTCCAC CCTAGGGGCG 
CTGGAGAGAG AACAGCTGGA CAGGCAGAAA GCAGAGGAAG AGGCAGCAGG CTCTAGGGAT 
GCCCTACAGC AGTGCAGGGG AAGCATACTC CGCCTCTCAT CCTCAGAAAG CCTGCTGAGG 
CGGGATCTGG AGGAGAGTCG AGATGCTTTG AAGAAGGTTT CTGCTCTTAA CTCTGCTCTG 
GCCTCAGACA AGAGAGCACT GAACCAGCAG CTGCTGCAGC TGGAAGGCAA ACTGTCAGAG 
AGCCAATCGC AGCTGCAAGC TGTGAGTTCA GAGTTCACCT GTCTGCAGAG AGACGTCAGA 
CCTCTCAGAC TGCAGAGGGA TCAGGATGCC GACGTCATCC GTCAGCTGAA AGAGCAGCAG 
GTGGAGGCTG AGAAAAACCT GGAGAGAAAG GAGAGAGAGC TTCAGCTATT GGAGGAGGAG 
AGAACAAGTG GTGAACAACA GCTGGAGGAG CTTCGCTCTC ATCACGTCTC GCTGTGCCAG 
GAGCTGAAGG AGTTGCAGGA GGAGTTGCAA CAGGCGAAAC AGGAGGCCTC TGTCAGTGTC 
CCTGCAGCTG CAGCTCAGCC AGCAGCAGCA GCTCCTCTCC CAGGTCGAGG TGGACCGCTG 
TCAGCTGAGC ACTCGCCTCC ATGCGCTGCA GGAGGCCAAG CTCACCTTAC AGGGTCTGTC 
TGTGAAATCA AATGTCTGAG AGGGGAGCTG CAGAGAGAGA CAGCCAGCAG AGAGAGAAGT 
GAGGGCGAGA AGGAGGTGCT GCAGGAAGAC CTGCAGAGAC TGACGGAAGA GGTGCAGGAG 
CTGAGGAGAA GAAAGGCAGA AAAGGAAGAG GAGAAAAAGA TGTGCCAAAG AGAACGAGAG 
ACACTGAGGG AGGAATTAAG GCTGAGAGAC GGGGAGCTGG AAGCCCTGAA GACACGCACT 
GAAAGGATGG AAGAGGAGCT GGAAGAAAAG GGACAACAGG TGGAGAGACT GAAGGAGGAG 
GTGACACAAC GAGCTACCCA AGTCAGCTTC CTAAATGAGA GAATAGTTGG CTTGGAAGAG 
GAAAAGAAGA TTCTGCTGGA GGAGGCAAAG AGTACTGAGG TCAGACTGCT GGAGGAGGAG 
GCGAAAAGGA AAGACGAGGA GGTGCAAAGA GATGGGGAGA CTGAAGCTCT CTGTGACAGA 
ATAGAGAAAT TAGAAAGAGA GAAGGAAGAA ATAGATAAGG AGGTGCACCA AATGAAGAGA 
AAAGCACAGG AGCAAGAGGA GAGATGGAGG TCAAATGATG AGGAGAGCCA GAATGAGATA 
AACAGACTAA GGGGAGAAAA CTCTTCCCTC CAGGAGCACA GAAAGTCAGA GAAGCGACAA 
ATAGAGAAAC TGCAAAATGA AAAAATGGAG GTGCTGGAGA GGCTACAAGA GAAAGTTGGA 
GAGGTGGAAC TGATGAAGGT AAAACTAAGT GTGGCTCAGG AGGAGAGTGA GGAGCTGAGG 
GAGGAGGTGC AGAGGAGGGA GCGCAGCCTG GAGAAACAGA TATGTGCAGT ACGAGAAAGG 
GAGGAGGAAA TGGAGAACCT GAGGGGGCGG CTGAGGTTGG TGAAGGAGCG GGAGGCGGAA 
GCAGAGAGAG AGCTCCAGGA AACTTCTGAC AAACTGAAAA AAAAAGAGGC GAGGGAGGAA 
CACCTGGAGG TGCTGCTTAG AGAAAACCGG GAACTTTTGA AGCAGGAGGA AAAAGGACGA 
GAAGAAAGAG AAGACAGGAT CTGCTCCTTG ATCAAACAAT TGCAGGAGGC CCAGGCTGTT 
AACCTGGGAG CAAAAAGAAG AAGCACAGAA AGGCAGAAGG CCCATGACAG ACTGCAGGAG 
GAGGTGAAGG ACTGGCAGGA GCAAGCAGAA CAAAGTGTGA AGGAGGTGGC AAAACTCAAA 
GAAATCCTGA AGGGGAGAAA TGAGGAGGTG AACCAGCTGA AAGCAATGCT TGAAGAAAAA 
GAAGAGGAGT GGAAAGAGCA GGAGGCTTTG AGTAAAACAG AGAAAGAAAG GAGGAGAAGG 
CTGGAAAACA AGCTGGTCGA GGTGGAGGAA GAGAACACCA GGTTAGAGAA GGAGGTGAGA 
CTGATGCAGG CAGTTGGAGA TAAAGATAGG GAGGCTCTGG CAAAGGCTGG AGAGGAGTTA 
AAAACATTGA AGGACAACCT GGTAAAAAGT GAAAACAAAG AGAGGGAGCT GGGAGAAACT 
GTGAGAAGAA TGGAAGAGGA GAAGATGATG CTGAGTAGCA ATTTGAGAGA GATGGAGGAC 
GAGATCAAAG CAGAGAAGGA GCAGTTGTGG AGAGTAAGGA AAGAGAGGAG CAGTCTGGAG 
GACAAACTAA AAGAAAAAGA GAGAGAAATA GATGAGTGCA GGGATTTGCT TAGAAGTCAG 
GAGGAAAAAC TTGAGGAGGT GAAAAAGATG GATAGAAAAA CATTGGAGAG TGAAAGAAAT 
CGGTATCTAC TGTTGGAGGA AAAAGGGAAG ACCAGGGTTA AAAAGCTTGA GGAGGAGAAG 
GAGGACCTTT TACTGCAGTT AATGAAAAGG GAAAAAGAAG TGACAGAACT CCAAGAGGAG 
GTGCAGAGGC AGAGGATCAC TGCAAGCAAG GAGCTCAATG AAAAGAAAGA GGAGGCATTT 
AATGTACAAA GGGAATGTGA GGAGGCGCAG GAAAAGCTAA AATCATTGAA AAGGGAAATG 
ATTTATCTCC AAGAGGAGGT GAAACAAAAG CAAGAAGAGA ATGAGGAGGT GCAGGAAAAA 
CTGCAATCAT TGAAGAGGGA AATGATTTAT CTCCAAGAGG AGGTGAAACA AAAGCAAGAA 
GAGAATGAGG AGGTGCAGGA AAAACTGCAA TCATTGAAGA GGGAAATGAT TTATCTCCAG 
GAGGAGGTGA AACAAAAGCA AGAAGACAAT GAGGAGGAGG TGCACCAGAA ACATTTAAAG 
AAAGAGGAAG AGTGGAGAAC TAACGATATG ATGAGCAGAA ATGACACCCA GGAGCTACAG 
AGGCAGAAAG ATGAGGCTCA AGAGGAGCTG CAGAGGAGCA GAAAGGAGAT CTACATACTT 
AGAGAGGAGC TAGAAAAGGA ACAAAGAGAA AAAAAGGACC TTCAAGATGA GATGAAAAAG 
AGGAGTGAAG AGGCTGGATT GCTAAGACAG AAAATAGAGG AGGAGTTCAG CAGCACCAAG 
AAGAGGGAGG AGACAGAAAA ACAAACAAGA GAGAAGACTG AGATAGAGTT GGCCGCTCTA 
AAGGAGGAGC TGCAAAGGGA ACACAGGGAG AAATTGGAGA CAGTGGAGGA GCTGAAGAAG 
AGGAGAGAGG ATGTCACTGT GCTTGAAGGA GAGGTGAAGG AGGAGTGCAT GAAGAAGGAG 
GAGGTGCAGG AAGATCTAAG AAGAGTGCAG CACAGCTTGG AGCAGATGTC TCACAGTCTG 
AGCTCCCTTC AGAGTCAGGT GTCAGAGCTG AGTATCAGCA AGGAGCGAGT AGAGGTGGAG 
AAGCAGCAGA TGAGAGAAGG CCTGCAGGTG GCGCTCAAGG AGATGGACTT GCTGAAAGTT 
CTCCTCCAGG AAAGCCATGC AGAAGGAGAA CTGCTGAGGA GCACACTGGA GGAGAAGAAG 
AAGGAGATGG GGAGGGTTCG ACAGGAGAAC CTGCAGATGG AAGAGAGGCA AGAGGAGCTG 
GAGGAGCTGA GAGCCAGGGT CAAAGCCCTG GAGAGAAGAA GGACGGAGAT GGCAGAGGAG 
CTAGATAGGG CAGATGAGAG GAGGAGGGAG GCTGAGGAGT GGTGGAGGAG CAAAGTGAAG 
GAAGTAGAAC GGCAGAAGGG GACGATGCAA AGCACTCTTT ACACAGAAAT ACAGACGCAA 
GGGGAGCTAC TGAAGGAGTC GAGGTCCAAG CTTCAGGACA GTTTGAGGGA GCTGGAGGAG 
GCGAGAATGG AGCTTAGCCG GAACAGAGCC TTGTTGGAGG AGCAGAAAAC TTTCACGATT 
GCTGACAGAG AGGATTCGGG GGAGCAGAGG AGCAGAGGCA GCCAAGAGGA AAGAGGGGAG 
GTGAAGCAGG AGGAGATCAG GAGGCAGCTC CAGCAGAGGG AGGCTCAGGT GGACAGTTTG 
ACTCTCAGAG TCCAGGAGCT GCAGGAAGAC AGAAATCAGC TCCGATTGGC TCTGGAACAA 
ACTGAGGCGC AGCTGAAAAA CGTCGCCACC CAGGTGGAGC AGAGCACGGG GGCGGAGCCT 
AACCCAGGAG AGACTGTAGG GGGCAGACTG GAGGTCCTGC AGAGACTGGT GGCTGAACTG 
GAGCTCCAAC GGACACGACT GCAGAAGAAG AACTTGAATC TAGAAAAGCA AAAAGACAGA 
CTGAAGAAAG ACAGAGACTT TCTGAGAGAC ACCCTGAGAC AGGTGGAGCT GGAGCGTGGG 
AGGCTCAGCC AGCAGCTGAC AGACAGCAGA GGATCTCAGG TGGAACAGGT GTGCGCTAAT 
GCCACAGAAG AAGATCGTCT GCGCAGCAAA GTGAAGGAGC TGGAGGAGCA GGTTAGTCAG 
CTTCGTCTTT CTTTAGCTAT GGACCAGCAG CAGAAGGCGG AGTTTATCCA GCAGTCATCC 
AGAAACAGCC AATGGCTGCT CTCACTAAGA CGGGACCTCA ATGATTCGCT GTCTGCTGTG 
ACCCGTCTAC CAATCCCGTC TGTCCTGGAG TCTGAGACAC AGAGGTTGGA CCGCAGCCTG 
AGGGAGGAGG AGCTTAGGAT TTCCTTGAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020568961.1
CDS	136..6228
Translation	MDSGLLIGWQQQKEELEREVCRLQEELAESRAEREELESRTRALQDRVRLRQTIHPSLGLSLEEEEEQRRWKKQLREGREREARQALLIHRLQNKVLEYRDQCQHLDLQLQDERRKTLHMEGVRDEHSNSLESALIRLEEEQHRSVSLADTNSLLREQLSQSEKFNQELKEELHRLTADWIRSTEKAEQRETDWQREKEDQRGHVGQQQNNWLSVWRSVVALRRQCHSVKTAADRDLWELRAEFSRLSSTLLSSCDSVCSTQSLAALHKPLSSTLLPLPSPPSSSPPLSSTLILPPPPPDSCSTQGSFSMGELELKEEEGEGSEPKLYEKTEALQQRTVELARSLQDEREEKEREVERHQETARRLQSVSQAVIRLARVVSSRSRPASTSPEDVLRADLSSMLAVLSQAESVLLRRSEELQGAELNLRRLGEVRTSLQLQVKQLQDDHQQLLARSQHTQQELTHTQSVLSSEKKVSTLGALEREQLDRQKAEEEAAGSRDALQQCRGSILRLSSSESLLRRDLEESRDALKKVSALNSALASDKRALNQQLLQLEGKLSESQSQLQAVSSEFTCLQRDVRPLRLQRDQDADVIRQLKEQQVEAEKNLERKERELQLLEEERTSGEQQLEELRSHHVSLCQELKELQEELQQAKQEASVSVPAAAAQPAAAAPLPGRGGPLSAEHSPPCAAGGQAHLTGSVCEIKCLRGELQRETASRERSEGEKEVLQEDLQRLTEEVQELRRRKAEKEEEKKMCQRERETLREELRLRDGELEALKTRTERMEEELEEKGQQVERLKEEVTQRATQVSFLNERIVGLEEEKKILLEEAKSTEVRLLEEEAKRKDEEVQRDGETEALCDRIEKLEREKEEIDKEVHQMKRKAQEQEERWRSNDEESQNEINRLRGENSSLQEHRKSEKRQIEKLQNEKMEVLERLQEKVGEVELMKVKLSVAQEESEELREEVQRRERSLEKQICAVREREEEMENLRGRLRLVKEREAEAERELQETSDKLKKKEAREEHLEVLLRENRELLKQEEKGREEREDRICSLIKQLQEAQAVNLGAKRRSTERQKAHDRLQEEVKDWQEQAEQSVKEVAKLKEILKGRNEEVNQLKAMLEEKEEEWKEQEALSKTEKERRRRLENKLVEVEEENTRLEKEVRLMQAVGDKDREALAKAGEELKTLKDNLVKSENKERELGETVRRMEEEKMMLSSNLREMEDEIKAEKEQLWRVRKERSSLEDKLKEKEREIDECRDLLRSQEEKLEEVKKMDRKTLESERNRYLLLEEKGKTRVKKLEEEKEDLLLQLMKREKEVTELQEEVQRQRITASKELNEKKEEAFNVQRECEEAQEKLKSLKREMIYLQEEVKQKQEENEEVQEKLQSLKREMIYLQEEVKQKQEENEEVQEKLQSLKREMIYLQEEVKQKQEDNEEEVHQKHLKKEEEWRTNDMMSRNDTQELQRQKDEAQEELQRSRKEIYILREELEKEQREKKDLQDEMKKRSEEAGLLRQKIEEEFSSTKKREETEKQTREKTEIELAALKEELQREHREKLETVEELKKRREDVTVLEGEVKEECMKKEEVQEDLRRVQHSLEQMSHSLSSLQSQVSELSISKERVEVEKQQMREGLQVALKEMDLLKVLLQESHAEGELLRSTLEEKKKEMGRVRQENLQMEERQEELEELRARVKALERRRTEMAEELDRADERRREAEEWWRSKVKEVERQKGTMQSTLYTEIQTQGELLKESRSKLQDSLRELEEARMELSRNRALLEEQKTFTIADREDSGEQRSRGSQEERGEVKQEEIRRQLQQREAQVDSLTLRVQELQEDRNQLRLALEQTEAQLKNVATQVEQSTGAEPNPGETVGGRLEVLQRLVAELELQRTRLQKKNLNLEKQKDRLKKDRDFLRDTLRQVELERGRLSQQLTDSRGSQVEQVCANATEEDRLRSKVKELEEQVSQLRLSLAMDQQQKAEFIQQSSRNSQWLLSLRRDLNDSLSAVTRLPIPSVLESETQRLDRSLREEELRISLS

Target ORF information:

RefSeq Version	XM_020713302.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes trichohyalin-like (LOC101166347), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020713302.1

ORF Insert Sequence:

ATGGATTCTG GGTTGCTGAT TGGCTGGCAG CAGCAGAAGG AGGAGCTCGA GCGGGAGGTA 
TGCCGTCTGC AGGAGGAGCT GGCAGAGAGT CGAGCGGAGC GGGAGGAGTT GGAGTCCAGA 
ACCCGAGCTC TGCAGGACAG GGTGAGGCTC CGTCAGACCA TACATCCGTC ACTTGGCCTG 
TCACTTGAGG AGGAGGAGGA GCAGAGGAGG TGGAAGAAGC AGCTTAGGGA GGGCAGGGAG 
AGAGAGGCCA GGCAGGCACT GCTGATACAT CGGCTGCAGA ACAAGGTTCT GGAGTACAGA 
GATCAGTGCC AGCATTTGGA CCTGCAGTTG CAAGATGAAC GGAGGAAGAC GCTCCACATG 
GAGGGGGTCC GGGATGAACA TAGCAACTCT CTGGAAAGCG CCCTCATCAG GCTGGAGGAG 
GAGCAGCACA GGTCCGTAAG TCTGGCTGAC ACCAACTCTC TTCTGCGGGA GCAGCTCAGC 
CAATCTGAGA AGTTCAACCA GGAGCTTAAG GAGGAACTCC ACAGACTGAC AGCTGATTGG 
ATCAGATCCA CGGAGAAGGC GGAGCAAAGA GAAACTGATT GGCAGAGAGA GAAAGAGGAT 
CAGCGGGGTC ACGTGGGTCA GCAGCAGAAC AACTGGCTGT CAGTTTGGAG ATCCGTGGTG 
GCTCTGAGGA GACAATGTCA CAGCGTGAAA ACAGCTGCCG ACAGAGACCT GTGGGAGCTC 
AGGGCCGAGT TTTCCCGTCT CTCCTCAACA CTCCTCTCCA GCTGTGACTC TGTCTGCTCC 
ACCCAGAGCC TGGCGGCTCT TCACAAGCCT TTGTCTTCAA CTTTGCTGCC TCTTCCTTCC 
CCACCATCCA GTTCTCCTCC TCTATCCTCA ACACTGATCC TCCCTCCTCC TCCTCCAGAC 
TCCTGCTCCA CTCAGGGATC CTTCTCCATG GGGGAGCTAG AACTCAAAGA GGAGGAGGGA 
GAAGGTTCTG AGCCTAAGCT CTATGAAAAG ACTGAAGCTT TGCAGCAGAG GACTGTGGAG 
CTTGCTCGCT CACTGCAGGA TGAGAGAGAG GAGAAGGAGA GGGAGGTGGA AAGACATCAA 
GAAACTGCAA GGAGACTCCA GTCTGTCAGT CAGGCTGTGA TCAGACTGGC CAGAGTCGTC 
AGCAGCAGAA GTCGGCCAGC GAGCACCTCC CCAGAAGACG TCCTGCGGGC GGATTTGTCC 
TCCATGCTGG CTGTGCTCTC ACAGGCGGAA AGCGTCCTGC TGAGGAGGAG CGAGGAGCTG 
CAGGGGGCGG AGCTAAACCT GCGACGTCTC GGTGAGGTGA GAACCAGCTT GCAGCTGCAG 
GTGAAGCAGC TTCAGGACGA CCACCAGCAG CTTCTGGCAC GCTCACAACA CACACAGCAG 
GAGCTCACGC ACACACAGAG CGTCCTCAGC AGCGAGAAAA AGGTCTCCAC CCTAGGGGCG 
CTGGAGAGAG AACAGCTGGA CAGGCAGAAA GCAGAGGAAG AGGCAGCAGG CTCTAGGGAT 
GCCCTACAGC AGTGCAGGGG AAGCATACTC CGCCTCTCAT CCTCAGAAAG CCTGCTGAGG 
CGGGATCTGG AGGAGAGTCG AGATGCTTTG AAGAAGGTTT CTGCTCTTAA CTCTGCTCTG 
GCCTCAGACA AGAGAGCACT GAACCAGCAG CTGCTGCAGC TGGAAGGCAA ACTGTCAGAG 
AGCCAATCGC AGCTGCAAGC TGTGAGTTCA GAGTTCACCT GTCTGCAGAG AGACGTCAGA 
CCTCTCAGAC TGCAGAGGGA TCAGGATGCC GACGTCATCC GTCAGCTGAA AGAGCAGCAG 
GTGGAGGCTG AGAAAAACCT GGAGAGAAAG GAGAGAGAGC TTCAGCTATT GGAGGAGGAG 
AGAACAAGTG GTGAACAACA GCTGGAGGAG CTTCGCTCTC ATCACGTCTC GCTGTGCCAG 
GAGCTGAAGG AGTTGCAGGA GGAGTTGCAA CAGGCGAAAC AGGAGGCCTC TGTCAGTGTC 
CCTGCAGCTG CAGCTCAGCC AGCAGCAGCA GCTCCTCTCC CAGGTCGAGG TGGACCGCTG 
TCAGCTGAGC ACTCGCCTCC ATGCGCTGCA GGAGGCCAAG CTCACCTTAC AGGGTCTGTC 
TGTGAAATCA AATGTCTGAG AGGGGAGCTG CAGAGAGAGA CAGCCAGCAG AGAGAGAAGT 
GAGGGCGAGA AGGAGGTGCT GCAGGAAGAC CTGCAGAGAC TGACGGAAGA GGTGCAGGAG 
CTGAGGAGAA GAAAGGCAGA AAAGGAAGAG GAGAAAAAGA TGTGCCAAAG AGAACGAGAG 
ACACTGAGGG AGGAATTAAG GCTGAGAGAC GGGGAGCTGG AAGCCCTGAA GACACGCACT 
GAAAGGATGG AAGAGGAGCT GGAAGAAAAG GGACAACAGG TGGAGAGACT GAAGGAGGAG 
GTGACACAAC GAGCTACCCA AGTCAGCTTC CTAAATGAGA GAATAGTTGG CTTGGAAGAG 
GAAAAGAAGA TTCTGCTGGA GGAGGCAAAG AGTACTGAGG TCAGACTGCT GGAGGAGGAG 
GCGAAAAGGA AAGACGAGGA GGTGCAAAGA GATGGGGAGA CTGAAGCTCT CTGTGACAGA 
ATAGAGAAAT TAGAAAGAGA GAAGGAAGAA ATAGATAAGG AGGTGCACCA AATGAAGAGA 
AAAGCACAGG AGCAAGAGGA GAGATGGAGG TCAAATGATG AGGAGAGCCA GAATGAGATA 
AACAGACTAA GGGGAGAAAA CTCTTCCCTC CAGGAGCACA GAAAGTCAGA GAAGCGACAA 
ATAGAGAAAC TGCAAAATGA AAAAATGGAG GTGCTGGAGA GGCTACAAGA GAAAGTTGGA 
GAGGTGGAAC TGATGAAGGT AAAACTAAGT GTGGCTCAGG AGGAGAGTGA GGAGCTGAGG 
GAGGAGGTGC AGAGGAGGGA GCGCAGCCTG GAGAAACAGA TATGTGCAGT ACGAGAAAGG 
GAGGAGGAAA TGGAGAACCT GAGGGGGCGG CTGAGGTTGG TGAAGGAGCG GGAGGCGGAA 
GCAGAGAGAG AGCTCCAGGA AACTTCTGAC AAACTGAAAA AAAAAGAGGC GAGGGAGGAA 
CACCTGGAGG TGCTGCTTAG AGAAAACCGG GAACTTTTGA AGCAGGAGGA AAAAGGACGA 
GAAGAAAGAG AAGACAGGAT CTGCTCCTTG ATCAAACAAT TGCAGGAGGC CCAGGCTGTT 
AACCTGGGAG CAAAAAGAAG AAGCACAGAA AGGCAGAAGG CCCATGACAG ACTGCAGGAG 
GAGGTGAAGG ACTGGCAGGA GCAAGCAGAA CAAAGTGTGA AGGAGGTGGC AAAACTCAAA 
GAAATCCTGA AGGGGAGAAA TGAGGAGGTG AACCAGCTGA AAGCAATGCT TGAAGAAAAA 
GAAGAGGAGT GGAAAGAGCA GGAGGCTTTG AGTAAAACAG AGAAAGAAAG GAGGAGAAGG 
CTGGAAAACA AGCTGGTCGA GGTGGAGGAA GAGAACACCA GGTTAGAGAA GGAGGTGAGA 
CTGATGCAGG CAGTTGGAGA TAAAGATAGG GAGGCTCTGG CAAAGGCTGG AGAGGAGTTA 
AAAACATTGA AGGACAACCT GGTAAAAAGT GAAAACAAAG AGAGGGAGCT GGGAGAAACT 
GTGAGAAGAA TGGAAGAGGA GAAGATGATG CTGAGTAGCA ATTTGAGAGA GATGGAGGAC 
GAGATCAAAG CAGAGAAGGA GCAGTTGTGG AGAGTAAGGA AAGAGAGGAG CAGTCTGGAG 
GACAAACTAA AAGAAAAAGA GAGAGAAATA GATGAGTGCA GGGATTTGCT TAGAAGTCAG 
GAGGAAAAAC TTGAGGAGGT GAAAAAGATG GATAGAAAAA CATTGGAGAG TGAAAGAAAT 
CGGTATCTAC TGTTGGAGGA AAAAGGGAAG ACCAGGGTTA AAAAGCTTGA GGAGGAGAAG 
GAGGACCTTT TACTGCAGTT AATGAAAAGG GAAAAAGAAG TGACAGAACT CCAAGAGGAG 
GTGCAGAGGC AGAGGATCAC TGCAAGCAAG GAGCTCAATG AAAAGAAAGA GGAGGCATTT 
AATGTACAAA GGGAATGTGA GGAGGCGCAG GAAAAGCTAA AATCATTGAA AAGGGAAATG 
ATTTATCTCC AAGAGGAGGT GAAACAAAAG CAAGAAGAGA ATGAGGAGGT GCAGGAAAAA 
CTGCAATCAT TGAAGAGGGA AATGATTTAT CTCCAAGAGG AGGTGAAACA AAAGCAAGAA 
GAGAATGAGG AGGTGCAGGA AAAACTGCAA TCATTGAAGA GGGAAATGAT TTATCTCCAG 
GAGGAGGTGA AACAAAAGCA AGAAGACAAT GAGGAGGAGG TGCACCAGAA ACATTTAAAG 
AAAGAGGAAG AGTGGAGAAC TAACGATATG ATGAGCAGAA ATGACACCCA GGAGCTACAG 
AGGCAGAAAG ATGAGGCTCA AGAGGAGCTG CAGAGGAGCA GAAAGGAGAT CTACATACTT 
AGAGAGGAGC TAGAAAAGGA ACAAAGAGAA AAAAAGGACC TTCAAGATGA GATGAAAAAG 
AGGAGTGAAG AGGCTGGATT GCTAAGACAG AAAATAGAGG AGGAGTTCAG CAGCACCAAG 
AAGAGGGAGG AGACAGAAAA ACAAACAAGA GAGAAGACTG AGATAGAGTT GGCCGCTCTA 
AAGGAGGAGC TGCAAAGGGA ACACAGGGAG AAATTGGAGA CAGTGGAGGA GCTGAAGAAG 
AGGAGAGAGG ATGTCACTGT GCTTGAAGGA GAGGTGAAGG AGGAGTGCAT GAAGAAGGAG 
GAGGTGCAGG AAGATCTAAG AAGAGTGCAG CACAGCTTGG AGCAGATGTC TCACAGTCTG 
AGCTCCCTTC AGAGTCAGGT GTCAGAGCTG AGTATCAGCA AGGAGCGAGT AGAGGTGGAG 
AAGCAGCAGA TGAGAGAAGG CCTGCAGGTG GCGCTCAAGG AGATGGACTT GCTGAAAGTT 
CTCCTCCAGG AAAGCCATGC AGAAGGAGAA CTGCTGAGGA GCACACTGGA GGAGAAGAAG 
AAGGAGATGG GGAGGGTTCG ACAGGAGAAC CTGCAGATGG AAGAGAGGCA AGAGGAGCTG 
GAGGAGCTGA GAGCCAGGGT CAAAGCCCTG GAGAGAAGAA GGACGGAGAT GGCAGAGGAG 
CTAGATAGGG CAGATGAGAG GAGGAGGGAG GCTGAGGAGT GGTGGAGGAG CAAAGTGAAG 
GAAGTAGAAC GGCAGAAGGG GACGATGCAA AGCACTCTTT ACACAGAAAT ACAGACGCAA 
GGGGAGCTAC TGAAGGAGTC GAGGTCCAAG CTTCAGGACA GTTTGAGGGA GCTGGAGGAG 
GCGAGAATGG AGCTTAGCCG GAACAGAGCC TTGTTGGAGG AGCAGAAAAC TTTCACGATT 
GCTGACAGAG AGGATTCGGG GGAGCAGAGG AGCAGAGGCA GCCAAGAGGA AAGAGGGGAG 
GTGAAGCAGG AGGAGATCAG GAGGCAGCTC CAGCAGAGGG AGGCTCAGGT GGACAGTTTG 
ACTCTCAGAG TCCAGGAGCT GCAGGAAGAC AGAAATCAGC TCCGATTGGC TCTGGAACAA 
ACTGAGGCGC AGCTGAAAAA CGTCGCCACC CAGGTGGAGC AGAGCACGGG GGCGGAGCCT 
AACCCAGGAG AGACTGTAGG GGGCAGACTG GAGGTCCTGC AGAGACTGGT GGCTGAACTG 
GAGCTCCAAC GGACACGACT GCAGAAGAAG AACTTGAATC TAGAAAAGCA AAAAGACAGA 
CTGAAGAAAG ACAGAGACTT TCTGAGAGAC ACCCTGAGAC AGGTGGAGCT GGAGCGTGGG 
AGGCTCAGCC AGCAGCTGAC AGACAGCAGA GGATCTCAGG TGGAACAGGT GTGCGCTAAT 
GCCACAGAAG AAGATCGTCT GCGCAGCAAA GTGAAGGAGC TGGAGGAGCA GGTTAGTCAG 
CTTCGTCTTT CTTTAGCTAT GGACCAGCAG CAGAAGGCGG AGTTTATCCA GCAGTCATCC 
AGAAACAGCC AATGGCTGCT CTCACTAAGA CGGGACCTCA ATGATTCGCT GTCTGCTGTG 
ACCCGTCTAC CAATCCCGTC TGTCCTGGAG TCTGAGACAC AGAGGTTGGA CCGCAGCCTG 
AGGGAGGAGG AGCTTAGGAT TTCCTTGAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101166347 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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