usp9x cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp9x
Entrez Gene ID	101166345
Full Name	probable ubiquitin carboxyl-terminal hydrolase FAF-X
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023950530.1	XP_023806298.1	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X6
XM_023950531.1	XP_023806299.1	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X7
XM_011489289.2	XP_011487591.2	LOW QUALITY PROTEIN: probable ubiquitin carboxyl-terminal hydrolase FAF-X
XM_023950524.1	XP_023806292.1	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X1
XM_023950527.1	XP_023806295.1	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X3
XM_023950529.1	XP_023806297.1	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X5
XM_023950525.1	XP_023806293.1	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X2
XM_023950528.1	XP_023806296.1	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X4

Pathways from BioSystems
Gene Ontology

The following usp9x gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp9x cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa113026	XM_023950524.1 Latest version!	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa113027	XM_023950525.1 Latest version!	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa113028	XM_023950527.1 Latest version!	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa113029	XM_023950528.1 Latest version!	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa113030	XM_023950529.1 Latest version!	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa113031	XM_023950530.1 Latest version!	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa113032	XM_023950531.1 Latest version!	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa75229	XM_011489289.2 Latest version!	Oryzias latipes probable ubiquitin carboxyl-terminal hydrolase FAF-X (LOC101166345), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp9x ( XM_023950524.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806292.1 Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa113026
Clone ID Related Accession (Same CDS sequence)	XM_023950524.1
Accession Version	XM_023950524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGAAAG AAAAGGCCAA TTTACATTGA AACGAACTTA 
GAAGGCCAAA CCCAGAGAAG TCCACACGTG TTTTACCGGC ACGACCTGAT CAACCAGCTG 
CAGCATAACC ACGCTCTGGT CACCCTCGTG GCCGAGAACC TCTCTGCTTA CATGGAGACT 
ATGAGGCAGC TATCCAAAGA AGAACAAGCA GAGTTTGATC CTCAGACAGT CAGACCAGGA 
AGCCGCTACA GTCACGTCCA AGAAGTTCAG GAGCGACTCA ACTTCCTGAG GTTTCTTTTA 
AAAGACGGCC AGCTGTGGCT GTGTGCCCCT CAAGCCAAGC AGATCTGGAA GTGTCTGGCT 
GAGAATGCTG TGTTTCTCTG CGATCGCGAA GCTTGCTTCA AATGGTACTC CAAGCTGATG 
GGGGATGAAC CAGACCTGGA TCCGGACATC AATAAGGACT TCTTTGAGAA TAACGTTCTG 
CAGTTGGATC CGTCTCTGCT GACGGAAAAT GGCATGAAGT GCTTTGAGAG GTTCTTCAAG 
GCCGTCAACT GCAGGGAGGG GAAGCTGGTT GCAAAGCGCA GGGCTTACAT GATGGATGAC 
TTGGAGCTAA TAGGCCTGGA CTATCTGTGG AGGGTGGTAA TTCAAGGAAG CGATGACATT 
GCTAGCCGAG CCATAGACCT GCTGAAAGAG ATTTACACCA ACCTTGGTCC CAAACTACAA 
GTCAATCAGG TTGAGATTCA CGAGGATTTC ATCCAGTCCT GCTTCGACCG TCTGAAGGCG 
TCTTATGATA CCCTTTGTGT TTTGGACGGA GACAAAGACA GCATTAACTG TGCTAGGCAG 
GAGGCCATCC GCATGGTACG CGTTCTGACT GTGCTCAAGG AGTATATCAA CGAGTGTGAC 
AGCGACTACC ACGAGGAGAG AACCATACTG CCCATGTCCA GAGCTTTCAG GGGAAAGCAC 
ATAACTTTGA TTGTGCGTTT CCCAAACCAA GGTCGCCAAG TAGATGATTT AGATATCTGG 
TCCCACACCA ACGACACGAT TGGCTCGGTG AGGCGGGCGA TTCTAAACAG AATAAAGGCT 
AATGCTGCGC ATACAAAGGT CGAGTTGTTT ATTGGGGGTG AGGTGGTGGA TCCAGCTGAT 
GACAGGAAGT TGATCGGGCA GCTCAGTCTG AAGGACAAAA CGTTGATCAC GGCCAAGCTG 
ACCCAGGTGA GTGCCAACAT GCCCTCAAGT CCAGACAGCT CCTCTGACTC GTCTACTGGC 
TCTCCTGGTA ACCATGGAAA CCACTACAGC GACGGGCCCA ACCCTGAGGT GGAAAGCTGT 
CTGCCTGGCG TGATTATGTC GCTGCATCTG CGGTACATCT CCTTCCTGTG GCAGATCGCT 
GATCTAGGCT GCAGCCTCAA CATGCCTCTA CTTAGAGATG GAGCTCGTGT TCTCATGAAG 
CTTATGCCTC CTGATAACAC CACAGTAGAG AATCTGAGAG CTGTGTGTTT GGACCATGCC 
AAGCTGGGAG AGAACAGCCT GAGTCCCTCA CTGGACTCCT GCTTCTTTGG CCCCTCCCCC 
TCACAAGTGC TCTACCTCAT AGAGGTCGTG TATGCTTTGC TGATGCCAGC CAGTGCCACT 
CTCGGTGAAG ACGCCAGCGA CTTCCAATAC AACTTCCTGA AGAGCGGCGG CCTGCCCTTG 
GTGCTGAGCA TGCTCACCAG AAACAACTTT CTCCCATCGG CAGACATGGA AACTCGAAGG 
GGCGCCTATG TCAACGCACT AAAGATCGCA AAGCTCCTAC TGACCGCTGT GGGTTTTGGA 
CACGTGAAGG CGGTAGCTGA GGCCTGCCAG CCAAACGCCG ACGGAAATAT TCCCGTCTCT 
CCAATTAATC AGGCAACGCA CGACCAAGCG CTGGTTCTTC AGAGCGCCCT GCAAAGCATC 
CCTAACCCGG CCTCAGAGTG CATGCTGCGT AATGTAGCCA TTCGCCTGGC CCAACAGATC 
TCTGACGAGA ACTCTATTTG CCATCAGAAC TTTTTCCAGG CGTCGAAGTA CATCCCAGAC 
ATTTGTGTAA TCCGAGCAAT ACAGAAAATC GTGTGGGCAT CAGGCTGTGG TACAGTGCAG 
CTCGTCTTTA GCTCCAATGA GGAGATCAGC AGGATATATG AGAAGACAAA TGCTGCCAAG 
GAACCAGATG GAGAGGATGA GCAGGTGTGC TGCGAGGCCT TGGAGGTCAT GACCCTGTGC 
TTTGCCCTCA TGCCCACGGC TCTGGACACA CTAAGCAAAG AGAAGGCGTG GCAGACTTTT 
ATCATAGACC TGTTACTACA CTGCCACAGC AAGTCCGTTC GACAAATGGC TCAGGAGCAG 
TTTTTCCTCA TGGCCACAAG ATGCTGCATG GGCCACCGAC CCCTCCTCTT CTTCATCACC 
CTGTTATTCA CTGTGTTGGG GAGCACGGCC AAAGAGAGAG CCAAACATGC GGGGGACTAC 
TTCACCTTGC TCAGACACCT GCTAAACTAT GCTTACAACA GCAACATCAA CCTGCCAAAC 
GCCGAGGTGC TGCTTAACAA TGAGATTGAC TGGCTCAAGA GAATACGGGA TGAGGTGAAG 
AGAACAGGAG AGACTGGTGT GGAGGAGACG ATCCTGGAGG GCCACCTTGG AGTCACCAAA 
GAGCTCCTTG CTTTTCAAAC ACCGGAAAAG AAGTACTACA TTGGCAGCGA GAAGGGAGGA 
GCCAACCTTA TAAAGGAGCT GATCGATGAC TTCATATTCC CAGCATCAAA TGTTTACCTG 
CAGTACATGA AGACGGGAGA GTTCCCCACA GAGCAGGCCA TCCCAGTCTG CAGCACTCCC 
GCCTCCATCA ATGCTGGCTT TGAGCTCCTG GTGGCTCTGG CCGTTGGCTG CATGCGCAAT 
CTGAAACAAA TAGTTGACAC CCTGACTGAT ATGTACTACT CAGGCGGGGA GACGTTGACA 
GAGTGGGAGT ACTTGCCTCC AGTTGGGCCA CGACCCAATA AAGGCTTTGT AGGTCTAAAG 
AACGCCGGTG CCACATGTTA CATGAACTCT GTCATTCAGC AACTGTACAT GATCCCTCCC 
ATTCGCAACG GCATTCTGGC TATTGAGGGA ACAGGCACGG ATGTGGACGA TGACATGTCG 
GGGGATGAGA AGCAGGAGAA TGAGAGTAAC GTCGATCCTC GTGATGAGGT CTTCAGCTAC 
CATCACCAGT TTGATGACAA ACCGTCCAGT AAGTCAGAGG ACAGGAAGGA GTACAACATC 
GGCGTGCTTC GTCACTTGCA GGTCATCTTT GGTCATCTTG CTGCATCCAG GCTGCAGTAC 
TACGTCCCAA GAGGCTTCTG GAAACAGTTT AGATTGTGGG GTGAGCCAGT CAACCTGAGG 
GAACAGCACG ATGCTCTGGA GTTTTTCAAC TCTTTGGTGG ACAGTTTGGA TGAAGCTCTG 
AAGGCTCTGG GTCACCCCGC CATGCTCAGT AAGGTGCTGG GAGGCTCTTT CGCCGATCAG 
AAAATCTGTC AAGGGTGCCC TCACAGGTAT GAGTGTGAGG AGTCGTTTAC AACACTTAAC 
GTGGACATCA GAAACCACCA AAACCTGTTG GACTCCATGG AGCAGTATGT CAAAGGAGAT 
CTGCTGGAAG GAGCAAATGC CTATCACTGT GAGAAGTGTA ATAAAAAGGT GGACACCGTG 
AAGCGTCTGT TGATCAAGAA GCTCCCACCC GTCCTGGCCA TTCAGCTGAA GCGCTTTGAC 
TACGACTGGG AGCGAGAATG TGCCATCAAG TTTAACGACT ACTTTGAGTT CCCCAGGGAG 
CTGGATATGG AGCCTTACAC GGTAGCGGGC GTGGCCAAAC TGGAGGGCGA CGACGTGAAC 
CCGGAGAACC AGGTCATACA GCAGAACGAG CCGTCTGAAT CCACACCGCC GGGCAGCTCC 
AAATATCGTC TGGTGGGAGT GCTGGTCCAC TCAGGCCAGG CCAGTGGCGG ACACTACTAT 
TCCTACATAA TCCAGAGGAA CGGAGGGGAC GGGGAGAAGA ACCGCTGGTA TAAGTTCGAC 
GACGGCGACG TCACTGAATG CAAGATGGAC GACGAGGAGG AGATGAAGAA CCAGTGCTTC 
GGTGGGGAGT ACATGGGCGA GGTGTTTGAT CATATGATGA AAAGGATGTC GTATCGGAGG 
CAAAAGCGCT GGTGGAACGC TTACATCCTG TTTTACGAGC GAATGGACTC GTTGGACAAG 
GACAGCGATC TCGTCAAATA CATCTCGGAC CTGACGATCT CCACCAAGCC ACATCAGGTC 
AAGATGCCCA CGGTCATCGA GTGCAGCGTC CGCAAGCAGA ACGTCCAGTT CATGCACAAC 
CGAATGCAAT ACAGCCTAGA ATATTTCCAG TTCATAAAGA AACTTCTGAC CTGTAACAGT 
GTCTATTTAA ACCCTCCTCC AGGGCAAGAC CACCTGCTGC CAGAGGCAGA GGAGATCGCG 
ATGATCAGCA CTCAGCTGGC TGCTAGGTTC CTCTTCAGCA CAGGTTTCCA CACCAAGAAA 
GTAGTACGGG GTCCTGCCAG TGACTGGTAT GACGCCCTCT GCATCCTGCT GAGACACAGT 
AAGAATGTTC GTTACTGGTT TGCACACAAC GTCCTGTTTG CGTACCCTAA CCGCTTCTCC 
GAGTATTTGC TGGAGTGCCC CAGCGCTGAG GTCCGCGGAG CATTTGCCAA GCTCATCGTC 
TTCATCGCTC ACTTCTCCCT GCAAGATGGC CCCTGCCCCT CCCCCACCGC CTCACCTGGA 
CCGTCTGCTC AGGGCTGTGA TAATCTCAGT TTAAGTGACC ACTTGTTGAG AGCTGTTCTA 
AACCTGCTTA GGAGAGAGGT TTCTGAACAT GGCCGACATC TACAGCAGTA CTTCAACCTC 
TTTGTCATGT ATGCCAATCT GGGCCTGGCA GAGAAGTCTC AGCTGCTGAA GCTCAACGTT 
CCCGCCACTT TCATGCTGGT CGCTCTAGAC GAGGGTCCCG GTCCCCCCAT CAAATACCAG 
TATGCAGAAC TGGGCAAGCT CTACACTGTG GTTTCCCAGC TGGTCCGCTG CTGTGAGGTG 
TCCTCGCGCA TGCAATCCTC CATCAATGGT AACCCTCCTC TTCCTAACCC GTATGGGGAT 
CCCAACCTGA CGGCTCCGGT GATGTCTGTG CAGCAGCTGG TGGCAGAAAT CCTGTTTGTG 
AGGACGAGCT ACGTCAAGAA GATCATCGAG GACTGCAGCA ACTCTGAGGA GACCGTGAAG 
CTCCTTAGAT TCTGCTGCTG GGAGAATCCT CAGTTCTCCT CCACTGTGCT CAGTGAACTG 
CTTTGGCAGG TGGCATATTC TTACACGTAT GAGCTGAGGC CTTACCTGGA CTTGCTGCTA 
CAGATCCTTC TCATCGAAGA CTCCTGGCAG ACGCACAGGA TCCACAATGT CCTAAAGGGC 
ATCCCTGATG ACAGAGACGG GCTGTTTGAC ACCATCCAGC GCTCCAAAAA CCATTACCAG 
AAGAGGGCCT ACCAGTGCAT CAAGTGCATG GTGGCCCTCT TTAGCAACTG CTCCGTGGCT 
TACCAGATCC TGCAGAGTAA TGGTGACCTG AAAAGGAAGT GGACGTGGGC GGTGGAGTGG 
TTGGGGGATG AGTTGGAGAG AAGGCCGTAC ACAGGAAACC CGCAGTACAC CTACAACAAC 
TGGTCCCCCC CAGTTCAGAG CAACGAGACC TCCAACGGGT ATTTCCTGGA GCGTTCACAC 
AGTGCACGTA TGACACTTGC CAAAGCCTGT GAACTCTGTC CTGAAGAGCT CAAGTGTACA 
CAGGAAAGCC CAGGGAAGGA GCCGGACGAA CAGGAAGCAC CTGATGATCA AGACTCCTCC 
CCTCCTGAAG ACACCTCTCT GTACCCACAT TCTCCTGGAA GCACCCAGTT TCAGCAGAAT 
AACCACCCCC ATGGGCAGCC TTACACCGGG CCTGCTGCCC AGCACATGAA CAACCCCCAG 
CGCCCCGGCC CAGCCTCCGC TCCGACTCCC GGCCCGGCCC AGACCCCCGC CTCAGGCCCC 
GGCACCGCCC CTGGTTCCGG CCCGCGAGCA CAAGAGCACT GGGAGGGCAC AGAGGAAGTC 
GCCCCCGCCC CCGCCTCCAC CCCAGCACCT GTCCCCCCGA AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806292.1
CDS	658..8505
Translation	MTATTRGSPVGGNDSQGQGQAPDAQSQPPLPQNQTSSPNSSNENSPVSPPDEQSQVDRPPQLEEEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKCEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMATNPHCKFHIYNGTRPSETVPAGAQLPDDELYARPPDPRSPKGWLVDLINKFGNLNGFQILHDRFMSGQALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPVIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHNPEEEEWLTAERMAEWIQQNHILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTMQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDQALNAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLRKKRPIYIETNLEGQTQRSPHVFYRHDLINQLQHNHALVTLVAENLSAYMETMRQLSKEEQAEFDPQTVRPGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVFLCDREACFKWYSKLMGDEPDLDPDINKDFFENNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQGSDDIASRAIDLLKEIYTNLGPKLQVNQVEIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQEAIRMVRVLTVLKEYINECDSDYHEERTILPMSRAFRGKHITLIVRFPNQGRQVDDLDIWSHTNDTIGSVRRAILNRIKANAAHTKVELFIGGEVVDPADDRKLIGQLSLKDKTLITAKLTQVSANMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHLRYISFLWQIADLGCSLNMPLLRDGARVLMKLMPPDNTTVENLRAVCLDHAKLGENSLSPSLDSCFFGPSPSQVLYLIEVVYALLMPASATLGEDASDFQYNFLKSGGLPLVLSMLTRNNFLPSADMETRRGAYVNALKIAKLLLTAVGFGHVKAVAEACQPNADGNIPVSPINQATHDQALVLQSALQSIPNPASECMLRNVAIRLAQQISDENSICHQNFFQASKYIPDICVIRAIQKIVWASGCGTVQLVFSSNEEISRIYEKTNAAKEPDGEDEQVCCEALEVMTLCFALMPTALDTLSKEKAWQTFIIDLLLHCHSKSVRQMAQEQFFLMATRCCMGHRPLLFFITLLFTVLGSTAKERAKHAGDYFTLLRHLLNYAYNSNINLPNAEVLLNNEIDWLKRIRDEVKRTGETGVEETILEGHLGVTKELLAFQTPEKKYYIGSEKGGANLIKELIDDFIFPASNVYLQYMKTGEFPTEQAIPVCSTPASINAGFELLVALAVGCMRNLKQIVDTLTDMYYSGGETLTEWEYLPPVGPRPNKGFVGLKNAGATCYMNSVIQQLYMIPPIRNGILAIEGTGTDVDDDMSGDEKQENESNVDPRDEVFSYHHQFDDKPSSKSEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSMEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDDVNPENQVIQQNEPSESTPPGSSKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDEEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDSLDKDSDLVKYISDLTISTKPHQVKMPTVIECSVRKQNVQFMHNRMQYSLEYFQFIKKLLTCNSVYLNPPPGQDHLLPEAEEIAMISTQLAARFLFSTGFHTKKVVRGPASDWYDALCILLRHSKNVRYWFAHNVLFAYPNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPTASPGPSAQGCDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGLAEKSQLLKLNVPATFMLVALDEGPGPPIKYQYAELGKLYTVVSQLVRCCEVSSRMQSSINGNPPLPNPYGDPNLTAPVMSVQQLVAEILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNVLKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCSVAYQILQSNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEELKCTQESPGKEPDEQEAPDDQDSSPPEDTSLYPHSPGSTQFQQNNHPHGQPYTGPAAQHMNNPQRPGPASAPTPGPAQTPASGPGTAPGSGPRAQEHWEGTEEVAPAPASTPAPVPPKE

Target ORF information:

RefSeq Version	XM_023950524.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950524.1

ORF Insert Sequence:

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGAAAG AAAAGGCCAA TTTACATTGA AACGAACTTA 
GAAGGCCAAA CCCAGAGAAG TCCACACGTG TTTTACCGGC ACGACCTGAT CAACCAGCTG 
CAGCATAACC ACGCTCTGGT CACCCTCGTG GCCGAGAACC TCTCTGCTTA CATGGAGACT 
ATGAGGCAGC TATCCAAAGA AGAACAAGCA GAGTTTGATC CTCAGACAGT CAGACCAGGA 
AGCCGCTACA GTCACGTCCA AGAAGTTCAG GAGCGACTCA ACTTCCTGAG GTTTCTTTTA 
AAAGACGGCC AGCTGTGGCT GTGTGCCCCT CAAGCCAAGC AGATCTGGAA GTGTCTGGCT 
GAGAATGCTG TGTTTCTCTG CGATCGCGAA GCTTGCTTCA AATGGTACTC CAAGCTGATG 
GGGGATGAAC CAGACCTGGA TCCGGACATC AATAAGGACT TCTTTGAGAA TAACGTTCTG 
CAGTTGGATC CGTCTCTGCT GACGGAAAAT GGCATGAAGT GCTTTGAGAG GTTCTTCAAG 
GCCGTCAACT GCAGGGAGGG GAAGCTGGTT GCAAAGCGCA GGGCTTACAT GATGGATGAC 
TTGGAGCTAA TAGGCCTGGA CTATCTGTGG AGGGTGGTAA TTCAAGGAAG CGATGACATT 
GCTAGCCGAG CCATAGACCT GCTGAAAGAG ATTTACACCA ACCTTGGTCC CAAACTACAA 
GTCAATCAGG TTGAGATTCA CGAGGATTTC ATCCAGTCCT GCTTCGACCG TCTGAAGGCG 
TCTTATGATA CCCTTTGTGT TTTGGACGGA GACAAAGACA GCATTAACTG TGCTAGGCAG 
GAGGCCATCC GCATGGTACG CGTTCTGACT GTGCTCAAGG AGTATATCAA CGAGTGTGAC 
AGCGACTACC ACGAGGAGAG AACCATACTG CCCATGTCCA GAGCTTTCAG GGGAAAGCAC 
ATAACTTTGA TTGTGCGTTT CCCAAACCAA GGTCGCCAAG TAGATGATTT AGATATCTGG 
TCCCACACCA ACGACACGAT TGGCTCGGTG AGGCGGGCGA TTCTAAACAG AATAAAGGCT 
AATGCTGCGC ATACAAAGGT CGAGTTGTTT ATTGGGGGTG AGGTGGTGGA TCCAGCTGAT 
GACAGGAAGT TGATCGGGCA GCTCAGTCTG AAGGACAAAA CGTTGATCAC GGCCAAGCTG 
ACCCAGGTGA GTGCCAACAT GCCCTCAAGT CCAGACAGCT CCTCTGACTC GTCTACTGGC 
TCTCCTGGTA ACCATGGAAA CCACTACAGC GACGGGCCCA ACCCTGAGGT GGAAAGCTGT 
CTGCCTGGCG TGATTATGTC GCTGCATCTG CGGTACATCT CCTTCCTGTG GCAGATCGCT 
GATCTAGGCT GCAGCCTCAA CATGCCTCTA CTTAGAGATG GAGCTCGTGT TCTCATGAAG 
CTTATGCCTC CTGATAACAC CACAGTAGAG AATCTGAGAG CTGTGTGTTT GGACCATGCC 
AAGCTGGGAG AGAACAGCCT GAGTCCCTCA CTGGACTCCT GCTTCTTTGG CCCCTCCCCC 
TCACAAGTGC TCTACCTCAT AGAGGTCGTG TATGCTTTGC TGATGCCAGC CAGTGCCACT 
CTCGGTGAAG ACGCCAGCGA CTTCCAATAC AACTTCCTGA AGAGCGGCGG CCTGCCCTTG 
GTGCTGAGCA TGCTCACCAG AAACAACTTT CTCCCATCGG CAGACATGGA AACTCGAAGG 
GGCGCCTATG TCAACGCACT AAAGATCGCA AAGCTCCTAC TGACCGCTGT GGGTTTTGGA 
CACGTGAAGG CGGTAGCTGA GGCCTGCCAG CCAAACGCCG ACGGAAATAT TCCCGTCTCT 
CCAATTAATC AGGCAACGCA CGACCAAGCG CTGGTTCTTC AGAGCGCCCT GCAAAGCATC 
CCTAACCCGG CCTCAGAGTG CATGCTGCGT AATGTAGCCA TTCGCCTGGC CCAACAGATC 
TCTGACGAGA ACTCTATTTG CCATCAGAAC TTTTTCCAGG CGTCGAAGTA CATCCCAGAC 
ATTTGTGTAA TCCGAGCAAT ACAGAAAATC GTGTGGGCAT CAGGCTGTGG TACAGTGCAG 
CTCGTCTTTA GCTCCAATGA GGAGATCAGC AGGATATATG AGAAGACAAA TGCTGCCAAG 
GAACCAGATG GAGAGGATGA GCAGGTGTGC TGCGAGGCCT TGGAGGTCAT GACCCTGTGC 
TTTGCCCTCA TGCCCACGGC TCTGGACACA CTAAGCAAAG AGAAGGCGTG GCAGACTTTT 
ATCATAGACC TGTTACTACA CTGCCACAGC AAGTCCGTTC GACAAATGGC TCAGGAGCAG 
TTTTTCCTCA TGGCCACAAG ATGCTGCATG GGCCACCGAC CCCTCCTCTT CTTCATCACC 
CTGTTATTCA CTGTGTTGGG GAGCACGGCC AAAGAGAGAG CCAAACATGC GGGGGACTAC 
TTCACCTTGC TCAGACACCT GCTAAACTAT GCTTACAACA GCAACATCAA CCTGCCAAAC 
GCCGAGGTGC TGCTTAACAA TGAGATTGAC TGGCTCAAGA GAATACGGGA TGAGGTGAAG 
AGAACAGGAG AGACTGGTGT GGAGGAGACG ATCCTGGAGG GCCACCTTGG AGTCACCAAA 
GAGCTCCTTG CTTTTCAAAC ACCGGAAAAG AAGTACTACA TTGGCAGCGA GAAGGGAGGA 
GCCAACCTTA TAAAGGAGCT GATCGATGAC TTCATATTCC CAGCATCAAA TGTTTACCTG 
CAGTACATGA AGACGGGAGA GTTCCCCACA GAGCAGGCCA TCCCAGTCTG CAGCACTCCC 
GCCTCCATCA ATGCTGGCTT TGAGCTCCTG GTGGCTCTGG CCGTTGGCTG CATGCGCAAT 
CTGAAACAAA TAGTTGACAC CCTGACTGAT ATGTACTACT CAGGCGGGGA GACGTTGACA 
GAGTGGGAGT ACTTGCCTCC AGTTGGGCCA CGACCCAATA AAGGCTTTGT AGGTCTAAAG 
AACGCCGGTG CCACATGTTA CATGAACTCT GTCATTCAGC AACTGTACAT GATCCCTCCC 
ATTCGCAACG GCATTCTGGC TATTGAGGGA ACAGGCACGG ATGTGGACGA TGACATGTCG 
GGGGATGAGA AGCAGGAGAA TGAGAGTAAC GTCGATCCTC GTGATGAGGT CTTCAGCTAC 
CATCACCAGT TTGATGACAA ACCGTCCAGT AAGTCAGAGG ACAGGAAGGA GTACAACATC 
GGCGTGCTTC GTCACTTGCA GGTCATCTTT GGTCATCTTG CTGCATCCAG GCTGCAGTAC 
TACGTCCCAA GAGGCTTCTG GAAACAGTTT AGATTGTGGG GTGAGCCAGT CAACCTGAGG 
GAACAGCACG ATGCTCTGGA GTTTTTCAAC TCTTTGGTGG ACAGTTTGGA TGAAGCTCTG 
AAGGCTCTGG GTCACCCCGC CATGCTCAGT AAGGTGCTGG GAGGCTCTTT CGCCGATCAG 
AAAATCTGTC AAGGGTGCCC TCACAGGTAT GAGTGTGAGG AGTCGTTTAC AACACTTAAC 
GTGGACATCA GAAACCACCA AAACCTGTTG GACTCCATGG AGCAGTATGT CAAAGGAGAT 
CTGCTGGAAG GAGCAAATGC CTATCACTGT GAGAAGTGTA ATAAAAAGGT GGACACCGTG 
AAGCGTCTGT TGATCAAGAA GCTCCCACCC GTCCTGGCCA TTCAGCTGAA GCGCTTTGAC 
TACGACTGGG AGCGAGAATG TGCCATCAAG TTTAACGACT ACTTTGAGTT CCCCAGGGAG 
CTGGATATGG AGCCTTACAC GGTAGCGGGC GTGGCCAAAC TGGAGGGCGA CGACGTGAAC 
CCGGAGAACC AGGTCATACA GCAGAACGAG CCGTCTGAAT CCACACCGCC GGGCAGCTCC 
AAATATCGTC TGGTGGGAGT GCTGGTCCAC TCAGGCCAGG CCAGTGGCGG ACACTACTAT 
TCCTACATAA TCCAGAGGAA CGGAGGGGAC GGGGAGAAGA ACCGCTGGTA TAAGTTCGAC 
GACGGCGACG TCACTGAATG CAAGATGGAC GACGAGGAGG AGATGAAGAA CCAGTGCTTC 
GGTGGGGAGT ACATGGGCGA GGTGTTTGAT CATATGATGA AAAGGATGTC GTATCGGAGG 
CAAAAGCGCT GGTGGAACGC TTACATCCTG TTTTACGAGC GAATGGACTC GTTGGACAAG 
GACAGCGATC TCGTCAAATA CATCTCGGAC CTGACGATCT CCACCAAGCC ACATCAGGTC 
AAGATGCCCA CGGTCATCGA GTGCAGCGTC CGCAAGCAGA ACGTCCAGTT CATGCACAAC 
CGAATGCAAT ACAGCCTAGA ATATTTCCAG TTCATAAAGA AACTTCTGAC CTGTAACAGT 
GTCTATTTAA ACCCTCCTCC AGGGCAAGAC CACCTGCTGC CAGAGGCAGA GGAGATCGCG 
ATGATCAGCA CTCAGCTGGC TGCTAGGTTC CTCTTCAGCA CAGGTTTCCA CACCAAGAAA 
GTAGTACGGG GTCCTGCCAG TGACTGGTAT GACGCCCTCT GCATCCTGCT GAGACACAGT 
AAGAATGTTC GTTACTGGTT TGCACACAAC GTCCTGTTTG CGTACCCTAA CCGCTTCTCC 
GAGTATTTGC TGGAGTGCCC CAGCGCTGAG GTCCGCGGAG CATTTGCCAA GCTCATCGTC 
TTCATCGCTC ACTTCTCCCT GCAAGATGGC CCCTGCCCCT CCCCCACCGC CTCACCTGGA 
CCGTCTGCTC AGGGCTGTGA TAATCTCAGT TTAAGTGACC ACTTGTTGAG AGCTGTTCTA 
AACCTGCTTA GGAGAGAGGT TTCTGAACAT GGCCGACATC TACAGCAGTA CTTCAACCTC 
TTTGTCATGT ATGCCAATCT GGGCCTGGCA GAGAAGTCTC AGCTGCTGAA GCTCAACGTT 
CCCGCCACTT TCATGCTGGT CGCTCTAGAC GAGGGTCCCG GTCCCCCCAT CAAATACCAG 
TATGCAGAAC TGGGCAAGCT CTACACTGTG GTTTCCCAGC TGGTCCGCTG CTGTGAGGTG 
TCCTCGCGCA TGCAATCCTC CATCAATGGT AACCCTCCTC TTCCTAACCC GTATGGGGAT 
CCCAACCTGA CGGCTCCGGT GATGTCTGTG CAGCAGCTGG TGGCAGAAAT CCTGTTTGTG 
AGGACGAGCT ACGTCAAGAA GATCATCGAG GACTGCAGCA ACTCTGAGGA GACCGTGAAG 
CTCCTTAGAT TCTGCTGCTG GGAGAATCCT CAGTTCTCCT CCACTGTGCT CAGTGAACTG 
CTTTGGCAGG TGGCATATTC TTACACGTAT GAGCTGAGGC CTTACCTGGA CTTGCTGCTA 
CAGATCCTTC TCATCGAAGA CTCCTGGCAG ACGCACAGGA TCCACAATGT CCTAAAGGGC 
ATCCCTGATG ACAGAGACGG GCTGTTTGAC ACCATCCAGC GCTCCAAAAA CCATTACCAG 
AAGAGGGCCT ACCAGTGCAT CAAGTGCATG GTGGCCCTCT TTAGCAACTG CTCCGTGGCT 
TACCAGATCC TGCAGAGTAA TGGTGACCTG AAAAGGAAGT GGACGTGGGC GGTGGAGTGG 
TTGGGGGATG AGTTGGAGAG AAGGCCGTAC ACAGGAAACC CGCAGTACAC CTACAACAAC 
TGGTCCCCCC CAGTTCAGAG CAACGAGACC TCCAACGGGT ATTTCCTGGA GCGTTCACAC 
AGTGCACGTA TGACACTTGC CAAAGCCTGT GAACTCTGTC CTGAAGAGCT CAAGTGTACA 
CAGGAAAGCC CAGGGAAGGA GCCGGACGAA CAGGAAGCAC CTGATGATCA AGACTCCTCC 
CCTCCTGAAG ACACCTCTCT GTACCCACAT TCTCCTGGAA GCACCCAGTT TCAGCAGAAT 
AACCACCCCC ATGGGCAGCC TTACACCGGG CCTGCTGCCC AGCACATGAA CAACCCCCAG 
CGCCCCGGCC CAGCCTCCGC TCCGACTCCC GGCCCGGCCC AGACCCCCGC CTCAGGCCCC 
GGCACCGCCC CTGGTTCCGG CCCGCGAGCA CAAGAGCACT GGGAGGGCAC AGAGGAAGTC 
GCCCCCGCCC CCGCCTCCAC CCCAGCACCT GTCCCCCCGA AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp9x ( XM_023950525.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806293.1 Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X2, mRNA.

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CloneID	OJa113027
Clone ID Related Accession (Same CDS sequence)	XM_023950525.1
Accession Version	XM_023950525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGAAAG AAAAGGCCAA TTTACATTGA AACGAACTTA 
GAAGGCCAAA CCCAGAGAAG TCCACACGTG TTTTACCGGC ACGACCTGAT CAACCAGCTG 
CAGCATAACC ACGCTCTGGT CACCCTCGTG GCCGAGAACC TCTCTGCTTA CATGGAGACT 
ATGAGGCAGC TATCCAAAGA ACAAGCAGAG TTTGATCCTC AGACAGTCAG ACCAGGAAGC 
CGCTACAGTC ACGTCCAAGA AGTTCAGGAG CGACTCAACT TCCTGAGGTT TCTTTTAAAA 
GACGGCCAGC TGTGGCTGTG TGCCCCTCAA GCCAAGCAGA TCTGGAAGTG TCTGGCTGAG 
AATGCTGTGT TTCTCTGCGA TCGCGAAGCT TGCTTCAAAT GGTACTCCAA GCTGATGGGG 
GATGAACCAG ACCTGGATCC GGACATCAAT AAGGACTTCT TTGAGAATAA CGTTCTGCAG 
TTGGATCCGT CTCTGCTGAC GGAAAATGGC ATGAAGTGCT TTGAGAGGTT CTTCAAGGCC 
GTCAACTGCA GGGAGGGGAA GCTGGTTGCA AAGCGCAGGG CTTACATGAT GGATGACTTG 
GAGCTAATAG GCCTGGACTA TCTGTGGAGG GTGGTAATTC AAGGAAGCGA TGACATTGCT 
AGCCGAGCCA TAGACCTGCT GAAAGAGATT TACACCAACC TTGGTCCCAA ACTACAAGTC 
AATCAGGTTG AGATTCACGA GGATTTCATC CAGTCCTGCT TCGACCGTCT GAAGGCGTCT 
TATGATACCC TTTGTGTTTT GGACGGAGAC AAAGACAGCA TTAACTGTGC TAGGCAGGAG 
GCCATCCGCA TGGTACGCGT TCTGACTGTG CTCAAGGAGT ATATCAACGA GTGTGACAGC 
GACTACCACG AGGAGAGAAC CATACTGCCC ATGTCCAGAG CTTTCAGGGG AAAGCACATA 
ACTTTGATTG TGCGTTTCCC AAACCAAGGT CGCCAAGTAG ATGATTTAGA TATCTGGTCC 
CACACCAACG ACACGATTGG CTCGGTGAGG CGGGCGATTC TAAACAGAAT AAAGGCTAAT 
GCTGCGCATA CAAAGGTCGA GTTGTTTATT GGGGGTGAGG TGGTGGATCC AGCTGATGAC 
AGGAAGTTGA TCGGGCAGCT CAGTCTGAAG GACAAAACGT TGATCACGGC CAAGCTGACC 
CAGGTGAGTG CCAACATGCC CTCAAGTCCA GACAGCTCCT CTGACTCGTC TACTGGCTCT 
CCTGGTAACC ATGGAAACCA CTACAGCGAC GGGCCCAACC CTGAGGTGGA AAGCTGTCTG 
CCTGGCGTGA TTATGTCGCT GCATCTGCGG TACATCTCCT TCCTGTGGCA GATCGCTGAT 
CTAGGCTGCA GCCTCAACAT GCCTCTACTT AGAGATGGAG CTCGTGTTCT CATGAAGCTT 
ATGCCTCCTG ATAACACCAC AGTAGAGAAT CTGAGAGCTG TGTGTTTGGA CCATGCCAAG 
CTGGGAGAGA ACAGCCTGAG TCCCTCACTG GACTCCTGCT TCTTTGGCCC CTCCCCCTCA 
CAAGTGCTCT ACCTCATAGA GGTCGTGTAT GCTTTGCTGA TGCCAGCCAG TGCCACTCTC 
GGTGAAGACG CCAGCGACTT CCAATACAAC TTCCTGAAGA GCGGCGGCCT GCCCTTGGTG 
CTGAGCATGC TCACCAGAAA CAACTTTCTC CCATCGGCAG ACATGGAAAC TCGAAGGGGC 
GCCTATGTCA ACGCACTAAA GATCGCAAAG CTCCTACTGA CCGCTGTGGG TTTTGGACAC 
GTGAAGGCGG TAGCTGAGGC CTGCCAGCCA AACGCCGACG GAAATATTCC CGTCTCTCCA 
ATTAATCAGG CAACGCACGA CCAAGCGCTG GTTCTTCAGA GCGCCCTGCA AAGCATCCCT 
AACCCGGCCT CAGAGTGCAT GCTGCGTAAT GTAGCCATTC GCCTGGCCCA ACAGATCTCT 
GACGAGAACT CTATTTGCCA TCAGAACTTT TTCCAGGCGT CGAAGTACAT CCCAGACATT 
TGTGTAATCC GAGCAATACA GAAAATCGTG TGGGCATCAG GCTGTGGTAC AGTGCAGCTC 
GTCTTTAGCT CCAATGAGGA GATCAGCAGG ATATATGAGA AGACAAATGC TGCCAAGGAA 
CCAGATGGAG AGGATGAGCA GGTGTGCTGC GAGGCCTTGG AGGTCATGAC CCTGTGCTTT 
GCCCTCATGC CCACGGCTCT GGACACACTA AGCAAAGAGA AGGCGTGGCA GACTTTTATC 
ATAGACCTGT TACTACACTG CCACAGCAAG TCCGTTCGAC AAATGGCTCA GGAGCAGTTT 
TTCCTCATGG CCACAAGATG CTGCATGGGC CACCGACCCC TCCTCTTCTT CATCACCCTG 
TTATTCACTG TGTTGGGGAG CACGGCCAAA GAGAGAGCCA AACATGCGGG GGACTACTTC 
ACCTTGCTCA GACACCTGCT AAACTATGCT TACAACAGCA ACATCAACCT GCCAAACGCC 
GAGGTGCTGC TTAACAATGA GATTGACTGG CTCAAGAGAA TACGGGATGA GGTGAAGAGA 
ACAGGAGAGA CTGGTGTGGA GGAGACGATC CTGGAGGGCC ACCTTGGAGT CACCAAAGAG 
CTCCTTGCTT TTCAAACACC GGAAAAGAAG TACTACATTG GCAGCGAGAA GGGAGGAGCC 
AACCTTATAA AGGAGCTGAT CGATGACTTC ATATTCCCAG CATCAAATGT TTACCTGCAG 
TACATGAAGA CGGGAGAGTT CCCCACAGAG CAGGCCATCC CAGTCTGCAG CACTCCCGCC 
TCCATCAATG CTGGCTTTGA GCTCCTGGTG GCTCTGGCCG TTGGCTGCAT GCGCAATCTG 
AAACAAATAG TTGACACCCT GACTGATATG TACTACTCAG GCGGGGAGAC GTTGACAGAG 
TGGGAGTACT TGCCTCCAGT TGGGCCACGA CCCAATAAAG GCTTTGTAGG TCTAAAGAAC 
GCCGGTGCCA CATGTTACAT GAACTCTGTC ATTCAGCAAC TGTACATGAT CCCTCCCATT 
CGCAACGGCA TTCTGGCTAT TGAGGGAACA GGCACGGATG TGGACGATGA CATGTCGGGG 
GATGAGAAGC AGGAGAATGA GAGTAACGTC GATCCTCGTG ATGAGGTCTT CAGCTACCAT 
CACCAGTTTG ATGACAAACC GTCCAGTAAG TCAGAGGACA GGAAGGAGTA CAACATCGGC 
GTGCTTCGTC ACTTGCAGGT CATCTTTGGT CATCTTGCTG CATCCAGGCT GCAGTACTAC 
GTCCCAAGAG GCTTCTGGAA ACAGTTTAGA TTGTGGGGTG AGCCAGTCAA CCTGAGGGAA 
CAGCACGATG CTCTGGAGTT TTTCAACTCT TTGGTGGACA GTTTGGATGA AGCTCTGAAG 
GCTCTGGGTC ACCCCGCCAT GCTCAGTAAG GTGCTGGGAG GCTCTTTCGC CGATCAGAAA 
ATCTGTCAAG GGTGCCCTCA CAGGTATGAG TGTGAGGAGT CGTTTACAAC ACTTAACGTG 
GACATCAGAA ACCACCAAAA CCTGTTGGAC TCCATGGAGC AGTATGTCAA AGGAGATCTG 
CTGGAAGGAG CAAATGCCTA TCACTGTGAG AAGTGTAATA AAAAGGTGGA CACCGTGAAG 
CGTCTGTTGA TCAAGAAGCT CCCACCCGTC CTGGCCATTC AGCTGAAGCG CTTTGACTAC 
GACTGGGAGC GAGAATGTGC CATCAAGTTT AACGACTACT TTGAGTTCCC CAGGGAGCTG 
GATATGGAGC CTTACACGGT AGCGGGCGTG GCCAAACTGG AGGGCGACGA CGTGAACCCG 
GAGAACCAGG TCATACAGCA GAACGAGCCG TCTGAATCCA CACCGCCGGG CAGCTCCAAA 
TATCGTCTGG TGGGAGTGCT GGTCCACTCA GGCCAGGCCA GTGGCGGACA CTACTATTCC 
TACATAATCC AGAGGAACGG AGGGGACGGG GAGAAGAACC GCTGGTATAA GTTCGACGAC 
GGCGACGTCA CTGAATGCAA GATGGACGAC GAGGAGGAGA TGAAGAACCA GTGCTTCGGT 
GGGGAGTACA TGGGCGAGGT GTTTGATCAT ATGATGAAAA GGATGTCGTA TCGGAGGCAA 
AAGCGCTGGT GGAACGCTTA CATCCTGTTT TACGAGCGAA TGGACTCGTT GGACAAGGAC 
AGCGATCTCG TCAAATACAT CTCGGACCTG ACGATCTCCA CCAAGCCACA TCAGGTCAAG 
ATGCCCACGG TCATCGAGTG CAGCGTCCGC AAGCAGAACG TCCAGTTCAT GCACAACCGA 
ATGCAATACA GCCTAGAATA TTTCCAGTTC ATAAAGAAAC TTCTGACCTG TAACAGTGTC 
TATTTAAACC CTCCTCCAGG GCAAGACCAC CTGCTGCCAG AGGCAGAGGA GATCGCGATG 
ATCAGCACTC AGCTGGCTGC TAGGTTCCTC TTCAGCACAG GTTTCCACAC CAAGAAAGTA 
GTACGGGGTC CTGCCAGTGA CTGGTATGAC GCCCTCTGCA TCCTGCTGAG ACACAGTAAG 
AATGTTCGTT ACTGGTTTGC ACACAACGTC CTGTTTGCGT ACCCTAACCG CTTCTCCGAG 
TATTTGCTGG AGTGCCCCAG CGCTGAGGTC CGCGGAGCAT TTGCCAAGCT CATCGTCTTC 
ATCGCTCACT TCTCCCTGCA AGATGGCCCC TGCCCCTCCC CCACCGCCTC ACCTGGACCG 
TCTGCTCAGG GCTGTGATAA TCTCAGTTTA AGTGACCACT TGTTGAGAGC TGTTCTAAAC 
CTGCTTAGGA GAGAGGTTTC TGAACATGGC CGACATCTAC AGCAGTACTT CAACCTCTTT 
GTCATGTATG CCAATCTGGG CCTGGCAGAG AAGTCTCAGC TGCTGAAGCT CAACGTTCCC 
GCCACTTTCA TGCTGGTCGC TCTAGACGAG GGTCCCGGTC CCCCCATCAA ATACCAGTAT 
GCAGAACTGG GCAAGCTCTA CACTGTGGTT TCCCAGCTGG TCCGCTGCTG TGAGGTGTCC 
TCGCGCATGC AATCCTCCAT CAATGGTAAC CCTCCTCTTC CTAACCCGTA TGGGGATCCC 
AACCTGACGG CTCCGGTGAT GTCTGTGCAG CAGCTGGTGG CAGAAATCCT GTTTGTGAGG 
ACGAGCTACG TCAAGAAGAT CATCGAGGAC TGCAGCAACT CTGAGGAGAC CGTGAAGCTC 
CTTAGATTCT GCTGCTGGGA GAATCCTCAG TTCTCCTCCA CTGTGCTCAG TGAACTGCTT 
TGGCAGGTGG CATATTCTTA CACGTATGAG CTGAGGCCTT ACCTGGACTT GCTGCTACAG 
ATCCTTCTCA TCGAAGACTC CTGGCAGACG CACAGGATCC ACAATGTCCT AAAGGGCATC 
CCTGATGACA GAGACGGGCT GTTTGACACC ATCCAGCGCT CCAAAAACCA TTACCAGAAG 
AGGGCCTACC AGTGCATCAA GTGCATGGTG GCCCTCTTTA GCAACTGCTC CGTGGCTTAC 
CAGATCCTGC AGAGTAATGG TGACCTGAAA AGGAAGTGGA CGTGGGCGGT GGAGTGGTTG 
GGGGATGAGT TGGAGAGAAG GCCGTACACA GGAAACCCGC AGTACACCTA CAACAACTGG 
TCCCCCCCAG TTCAGAGCAA CGAGACCTCC AACGGGTATT TCCTGGAGCG TTCACACAGT 
GCACGTATGA CACTTGCCAA AGCCTGTGAA CTCTGTCCTG AAGAGCTCAA GTGTACACAG 
GAAAGCCCAG GGAAGGAGCC GGACGAACAG GAAGCACCTG ATGATCAAGA CTCCTCCCCT 
CCTGAAGACA CCTCTCTGTA CCCACATTCT CCTGGAAGCA CCCAGTTTCA GCAGAATAAC 
CACCCCCATG GGCAGCCTTA CACCGGGCCT GCTGCCCAGC ACATGAACAA CCCCCAGCGC 
CCCGGCCCAG CCTCCGCTCC GACTCCCGGC CCGGCCCAGA CCCCCGCCTC AGGCCCCGGC 
ACCGCCCCTG GTTCCGGCCC GCGAGCACAA GAGCACTGGG AGGGCACAGA GGAAGTCGCC 
CCCGCCCCCG CCTCCACCCC AGCACCTGTC CCCCCGAAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806293.1
CDS	658..8502
Translation	MTATTRGSPVGGNDSQGQGQAPDAQSQPPLPQNQTSSPNSSNENSPVSPPDEQSQVDRPPQLEEEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKCEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMATNPHCKFHIYNGTRPSETVPAGAQLPDDELYARPPDPRSPKGWLVDLINKFGNLNGFQILHDRFMSGQALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPVIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHNPEEEEWLTAERMAEWIQQNHILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTMQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDQALNAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLRKKRPIYIETNLEGQTQRSPHVFYRHDLINQLQHNHALVTLVAENLSAYMETMRQLSKEQAEFDPQTVRPGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVFLCDREACFKWYSKLMGDEPDLDPDINKDFFENNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQGSDDIASRAIDLLKEIYTNLGPKLQVNQVEIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQEAIRMVRVLTVLKEYINECDSDYHEERTILPMSRAFRGKHITLIVRFPNQGRQVDDLDIWSHTNDTIGSVRRAILNRIKANAAHTKVELFIGGEVVDPADDRKLIGQLSLKDKTLITAKLTQVSANMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHLRYISFLWQIADLGCSLNMPLLRDGARVLMKLMPPDNTTVENLRAVCLDHAKLGENSLSPSLDSCFFGPSPSQVLYLIEVVYALLMPASATLGEDASDFQYNFLKSGGLPLVLSMLTRNNFLPSADMETRRGAYVNALKIAKLLLTAVGFGHVKAVAEACQPNADGNIPVSPINQATHDQALVLQSALQSIPNPASECMLRNVAIRLAQQISDENSICHQNFFQASKYIPDICVIRAIQKIVWASGCGTVQLVFSSNEEISRIYEKTNAAKEPDGEDEQVCCEALEVMTLCFALMPTALDTLSKEKAWQTFIIDLLLHCHSKSVRQMAQEQFFLMATRCCMGHRPLLFFITLLFTVLGSTAKERAKHAGDYFTLLRHLLNYAYNSNINLPNAEVLLNNEIDWLKRIRDEVKRTGETGVEETILEGHLGVTKELLAFQTPEKKYYIGSEKGGANLIKELIDDFIFPASNVYLQYMKTGEFPTEQAIPVCSTPASINAGFELLVALAVGCMRNLKQIVDTLTDMYYSGGETLTEWEYLPPVGPRPNKGFVGLKNAGATCYMNSVIQQLYMIPPIRNGILAIEGTGTDVDDDMSGDEKQENESNVDPRDEVFSYHHQFDDKPSSKSEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSMEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDDVNPENQVIQQNEPSESTPPGSSKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDEEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDSLDKDSDLVKYISDLTISTKPHQVKMPTVIECSVRKQNVQFMHNRMQYSLEYFQFIKKLLTCNSVYLNPPPGQDHLLPEAEEIAMISTQLAARFLFSTGFHTKKVVRGPASDWYDALCILLRHSKNVRYWFAHNVLFAYPNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPTASPGPSAQGCDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGLAEKSQLLKLNVPATFMLVALDEGPGPPIKYQYAELGKLYTVVSQLVRCCEVSSRMQSSINGNPPLPNPYGDPNLTAPVMSVQQLVAEILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNVLKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCSVAYQILQSNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEELKCTQESPGKEPDEQEAPDDQDSSPPEDTSLYPHSPGSTQFQQNNHPHGQPYTGPAAQHMNNPQRPGPASAPTPGPAQTPASGPGTAPGSGPRAQEHWEGTEEVAPAPASTPAPVPPKE

Target ORF information:

RefSeq Version	XM_023950525.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950525.1

ORF Insert Sequence:

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGAAAG AAAAGGCCAA TTTACATTGA AACGAACTTA 
GAAGGCCAAA CCCAGAGAAG TCCACACGTG TTTTACCGGC ACGACCTGAT CAACCAGCTG 
CAGCATAACC ACGCTCTGGT CACCCTCGTG GCCGAGAACC TCTCTGCTTA CATGGAGACT 
ATGAGGCAGC TATCCAAAGA ACAAGCAGAG TTTGATCCTC AGACAGTCAG ACCAGGAAGC 
CGCTACAGTC ACGTCCAAGA AGTTCAGGAG CGACTCAACT TCCTGAGGTT TCTTTTAAAA 
GACGGCCAGC TGTGGCTGTG TGCCCCTCAA GCCAAGCAGA TCTGGAAGTG TCTGGCTGAG 
AATGCTGTGT TTCTCTGCGA TCGCGAAGCT TGCTTCAAAT GGTACTCCAA GCTGATGGGG 
GATGAACCAG ACCTGGATCC GGACATCAAT AAGGACTTCT TTGAGAATAA CGTTCTGCAG 
TTGGATCCGT CTCTGCTGAC GGAAAATGGC ATGAAGTGCT TTGAGAGGTT CTTCAAGGCC 
GTCAACTGCA GGGAGGGGAA GCTGGTTGCA AAGCGCAGGG CTTACATGAT GGATGACTTG 
GAGCTAATAG GCCTGGACTA TCTGTGGAGG GTGGTAATTC AAGGAAGCGA TGACATTGCT 
AGCCGAGCCA TAGACCTGCT GAAAGAGATT TACACCAACC TTGGTCCCAA ACTACAAGTC 
AATCAGGTTG AGATTCACGA GGATTTCATC CAGTCCTGCT TCGACCGTCT GAAGGCGTCT 
TATGATACCC TTTGTGTTTT GGACGGAGAC AAAGACAGCA TTAACTGTGC TAGGCAGGAG 
GCCATCCGCA TGGTACGCGT TCTGACTGTG CTCAAGGAGT ATATCAACGA GTGTGACAGC 
GACTACCACG AGGAGAGAAC CATACTGCCC ATGTCCAGAG CTTTCAGGGG AAAGCACATA 
ACTTTGATTG TGCGTTTCCC AAACCAAGGT CGCCAAGTAG ATGATTTAGA TATCTGGTCC 
CACACCAACG ACACGATTGG CTCGGTGAGG CGGGCGATTC TAAACAGAAT AAAGGCTAAT 
GCTGCGCATA CAAAGGTCGA GTTGTTTATT GGGGGTGAGG TGGTGGATCC AGCTGATGAC 
AGGAAGTTGA TCGGGCAGCT CAGTCTGAAG GACAAAACGT TGATCACGGC CAAGCTGACC 
CAGGTGAGTG CCAACATGCC CTCAAGTCCA GACAGCTCCT CTGACTCGTC TACTGGCTCT 
CCTGGTAACC ATGGAAACCA CTACAGCGAC GGGCCCAACC CTGAGGTGGA AAGCTGTCTG 
CCTGGCGTGA TTATGTCGCT GCATCTGCGG TACATCTCCT TCCTGTGGCA GATCGCTGAT 
CTAGGCTGCA GCCTCAACAT GCCTCTACTT AGAGATGGAG CTCGTGTTCT CATGAAGCTT 
ATGCCTCCTG ATAACACCAC AGTAGAGAAT CTGAGAGCTG TGTGTTTGGA CCATGCCAAG 
CTGGGAGAGA ACAGCCTGAG TCCCTCACTG GACTCCTGCT TCTTTGGCCC CTCCCCCTCA 
CAAGTGCTCT ACCTCATAGA GGTCGTGTAT GCTTTGCTGA TGCCAGCCAG TGCCACTCTC 
GGTGAAGACG CCAGCGACTT CCAATACAAC TTCCTGAAGA GCGGCGGCCT GCCCTTGGTG 
CTGAGCATGC TCACCAGAAA CAACTTTCTC CCATCGGCAG ACATGGAAAC TCGAAGGGGC 
GCCTATGTCA ACGCACTAAA GATCGCAAAG CTCCTACTGA CCGCTGTGGG TTTTGGACAC 
GTGAAGGCGG TAGCTGAGGC CTGCCAGCCA AACGCCGACG GAAATATTCC CGTCTCTCCA 
ATTAATCAGG CAACGCACGA CCAAGCGCTG GTTCTTCAGA GCGCCCTGCA AAGCATCCCT 
AACCCGGCCT CAGAGTGCAT GCTGCGTAAT GTAGCCATTC GCCTGGCCCA ACAGATCTCT 
GACGAGAACT CTATTTGCCA TCAGAACTTT TTCCAGGCGT CGAAGTACAT CCCAGACATT 
TGTGTAATCC GAGCAATACA GAAAATCGTG TGGGCATCAG GCTGTGGTAC AGTGCAGCTC 
GTCTTTAGCT CCAATGAGGA GATCAGCAGG ATATATGAGA AGACAAATGC TGCCAAGGAA 
CCAGATGGAG AGGATGAGCA GGTGTGCTGC GAGGCCTTGG AGGTCATGAC CCTGTGCTTT 
GCCCTCATGC CCACGGCTCT GGACACACTA AGCAAAGAGA AGGCGTGGCA GACTTTTATC 
ATAGACCTGT TACTACACTG CCACAGCAAG TCCGTTCGAC AAATGGCTCA GGAGCAGTTT 
TTCCTCATGG CCACAAGATG CTGCATGGGC CACCGACCCC TCCTCTTCTT CATCACCCTG 
TTATTCACTG TGTTGGGGAG CACGGCCAAA GAGAGAGCCA AACATGCGGG GGACTACTTC 
ACCTTGCTCA GACACCTGCT AAACTATGCT TACAACAGCA ACATCAACCT GCCAAACGCC 
GAGGTGCTGC TTAACAATGA GATTGACTGG CTCAAGAGAA TACGGGATGA GGTGAAGAGA 
ACAGGAGAGA CTGGTGTGGA GGAGACGATC CTGGAGGGCC ACCTTGGAGT CACCAAAGAG 
CTCCTTGCTT TTCAAACACC GGAAAAGAAG TACTACATTG GCAGCGAGAA GGGAGGAGCC 
AACCTTATAA AGGAGCTGAT CGATGACTTC ATATTCCCAG CATCAAATGT TTACCTGCAG 
TACATGAAGA CGGGAGAGTT CCCCACAGAG CAGGCCATCC CAGTCTGCAG CACTCCCGCC 
TCCATCAATG CTGGCTTTGA GCTCCTGGTG GCTCTGGCCG TTGGCTGCAT GCGCAATCTG 
AAACAAATAG TTGACACCCT GACTGATATG TACTACTCAG GCGGGGAGAC GTTGACAGAG 
TGGGAGTACT TGCCTCCAGT TGGGCCACGA CCCAATAAAG GCTTTGTAGG TCTAAAGAAC 
GCCGGTGCCA CATGTTACAT GAACTCTGTC ATTCAGCAAC TGTACATGAT CCCTCCCATT 
CGCAACGGCA TTCTGGCTAT TGAGGGAACA GGCACGGATG TGGACGATGA CATGTCGGGG 
GATGAGAAGC AGGAGAATGA GAGTAACGTC GATCCTCGTG ATGAGGTCTT CAGCTACCAT 
CACCAGTTTG ATGACAAACC GTCCAGTAAG TCAGAGGACA GGAAGGAGTA CAACATCGGC 
GTGCTTCGTC ACTTGCAGGT CATCTTTGGT CATCTTGCTG CATCCAGGCT GCAGTACTAC 
GTCCCAAGAG GCTTCTGGAA ACAGTTTAGA TTGTGGGGTG AGCCAGTCAA CCTGAGGGAA 
CAGCACGATG CTCTGGAGTT TTTCAACTCT TTGGTGGACA GTTTGGATGA AGCTCTGAAG 
GCTCTGGGTC ACCCCGCCAT GCTCAGTAAG GTGCTGGGAG GCTCTTTCGC CGATCAGAAA 
ATCTGTCAAG GGTGCCCTCA CAGGTATGAG TGTGAGGAGT CGTTTACAAC ACTTAACGTG 
GACATCAGAA ACCACCAAAA CCTGTTGGAC TCCATGGAGC AGTATGTCAA AGGAGATCTG 
CTGGAAGGAG CAAATGCCTA TCACTGTGAG AAGTGTAATA AAAAGGTGGA CACCGTGAAG 
CGTCTGTTGA TCAAGAAGCT CCCACCCGTC CTGGCCATTC AGCTGAAGCG CTTTGACTAC 
GACTGGGAGC GAGAATGTGC CATCAAGTTT AACGACTACT TTGAGTTCCC CAGGGAGCTG 
GATATGGAGC CTTACACGGT AGCGGGCGTG GCCAAACTGG AGGGCGACGA CGTGAACCCG 
GAGAACCAGG TCATACAGCA GAACGAGCCG TCTGAATCCA CACCGCCGGG CAGCTCCAAA 
TATCGTCTGG TGGGAGTGCT GGTCCACTCA GGCCAGGCCA GTGGCGGACA CTACTATTCC 
TACATAATCC AGAGGAACGG AGGGGACGGG GAGAAGAACC GCTGGTATAA GTTCGACGAC 
GGCGACGTCA CTGAATGCAA GATGGACGAC GAGGAGGAGA TGAAGAACCA GTGCTTCGGT 
GGGGAGTACA TGGGCGAGGT GTTTGATCAT ATGATGAAAA GGATGTCGTA TCGGAGGCAA 
AAGCGCTGGT GGAACGCTTA CATCCTGTTT TACGAGCGAA TGGACTCGTT GGACAAGGAC 
AGCGATCTCG TCAAATACAT CTCGGACCTG ACGATCTCCA CCAAGCCACA TCAGGTCAAG 
ATGCCCACGG TCATCGAGTG CAGCGTCCGC AAGCAGAACG TCCAGTTCAT GCACAACCGA 
ATGCAATACA GCCTAGAATA TTTCCAGTTC ATAAAGAAAC TTCTGACCTG TAACAGTGTC 
TATTTAAACC CTCCTCCAGG GCAAGACCAC CTGCTGCCAG AGGCAGAGGA GATCGCGATG 
ATCAGCACTC AGCTGGCTGC TAGGTTCCTC TTCAGCACAG GTTTCCACAC CAAGAAAGTA 
GTACGGGGTC CTGCCAGTGA CTGGTATGAC GCCCTCTGCA TCCTGCTGAG ACACAGTAAG 
AATGTTCGTT ACTGGTTTGC ACACAACGTC CTGTTTGCGT ACCCTAACCG CTTCTCCGAG 
TATTTGCTGG AGTGCCCCAG CGCTGAGGTC CGCGGAGCAT TTGCCAAGCT CATCGTCTTC 
ATCGCTCACT TCTCCCTGCA AGATGGCCCC TGCCCCTCCC CCACCGCCTC ACCTGGACCG 
TCTGCTCAGG GCTGTGATAA TCTCAGTTTA AGTGACCACT TGTTGAGAGC TGTTCTAAAC 
CTGCTTAGGA GAGAGGTTTC TGAACATGGC CGACATCTAC AGCAGTACTT CAACCTCTTT 
GTCATGTATG CCAATCTGGG CCTGGCAGAG AAGTCTCAGC TGCTGAAGCT CAACGTTCCC 
GCCACTTTCA TGCTGGTCGC TCTAGACGAG GGTCCCGGTC CCCCCATCAA ATACCAGTAT 
GCAGAACTGG GCAAGCTCTA CACTGTGGTT TCCCAGCTGG TCCGCTGCTG TGAGGTGTCC 
TCGCGCATGC AATCCTCCAT CAATGGTAAC CCTCCTCTTC CTAACCCGTA TGGGGATCCC 
AACCTGACGG CTCCGGTGAT GTCTGTGCAG CAGCTGGTGG CAGAAATCCT GTTTGTGAGG 
ACGAGCTACG TCAAGAAGAT CATCGAGGAC TGCAGCAACT CTGAGGAGAC CGTGAAGCTC 
CTTAGATTCT GCTGCTGGGA GAATCCTCAG TTCTCCTCCA CTGTGCTCAG TGAACTGCTT 
TGGCAGGTGG CATATTCTTA CACGTATGAG CTGAGGCCTT ACCTGGACTT GCTGCTACAG 
ATCCTTCTCA TCGAAGACTC CTGGCAGACG CACAGGATCC ACAATGTCCT AAAGGGCATC 
CCTGATGACA GAGACGGGCT GTTTGACACC ATCCAGCGCT CCAAAAACCA TTACCAGAAG 
AGGGCCTACC AGTGCATCAA GTGCATGGTG GCCCTCTTTA GCAACTGCTC CGTGGCTTAC 
CAGATCCTGC AGAGTAATGG TGACCTGAAA AGGAAGTGGA CGTGGGCGGT GGAGTGGTTG 
GGGGATGAGT TGGAGAGAAG GCCGTACACA GGAAACCCGC AGTACACCTA CAACAACTGG 
TCCCCCCCAG TTCAGAGCAA CGAGACCTCC AACGGGTATT TCCTGGAGCG TTCACACAGT 
GCACGTATGA CACTTGCCAA AGCCTGTGAA CTCTGTCCTG AAGAGCTCAA GTGTACACAG 
GAAAGCCCAG GGAAGGAGCC GGACGAACAG GAAGCACCTG ATGATCAAGA CTCCTCCCCT 
CCTGAAGACA CCTCTCTGTA CCCACATTCT CCTGGAAGCA CCCAGTTTCA GCAGAATAAC 
CACCCCCATG GGCAGCCTTA CACCGGGCCT GCTGCCCAGC ACATGAACAA CCCCCAGCGC 
CCCGGCCCAG CCTCCGCTCC GACTCCCGGC CCGGCCCAGA CCCCCGCCTC AGGCCCCGGC 
ACCGCCCCTG GTTCCGGCCC GCGAGCACAA GAGCACTGGG AGGGCACAGA GGAAGTCGCC 
CCCGCCCCCG CCTCCACCCC AGCACCTGTC CCCCCGAAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp9x ( XM_023950527.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806295.1 Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X3, mRNA.

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CloneID	OJa113028
Clone ID Related Accession (Same CDS sequence)	XM_023950527.1
Accession Version	XM_023950527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGAAAG AAAAGGCCAA TTTACATTGA AACGAACTTA 
GAAGGCCAAA CCCAGAGAAG TCCACACGTG TTTTACCGGC ACGACCTGAT CAACCAGCTG 
CAGCATAACC ACGCTCTGGT CACCCTCGTG GCCGAGAACC TCTCTGCTTA CATGGAGACT 
ATGAGGCAGC TATCCAAAGA AGAACAAGCA GAGTTTGATC CTCAGACAGT CAGACCAGGA 
AGCCGCTACA GTCACGTCCA AGAAGTTCAG GAGCGACTCA ACTTCCTGAG GTTTCTTTTA 
AAAGACGGCC AGCTGTGGCT GTGTGCCCCT CAAGCCAAGC AGATCTGGAA GTGTCTGGCT 
GAGAATGCTG TGTTTCTCTG CGATCGCGAA GCTTGCTTCA AATGGTACTC CAAGCTGATG 
GGGGATGAAC CAGACCTGGA TCCGGACATC AATAAGGACT TCTTTGAGAA TAACGTTCTG 
CAGTTGGATC CGTCTCTGCT GACGGAAAAT GGCATGAAGT GCTTTGAGAG GTTCTTCAAG 
GCCGTCAACT GCAGGGAGGG GAAGCTGGTT GCAAAGCGCA GGGCTTACAT GATGGATGAC 
TTGGAGCTAA TAGGCCTGGA CTATCTGTGG AGGGTGGTAA TTCAAGGAAG CGATGACATT 
GCTAGCCGAG CCATAGACCT GCTGAAAGAG ATTTACACCA ACCTTGGTCC CAAACTACAA 
GTCAATCAGG TTGAGATTCA CGAGGATTTC ATCCAGTCCT GCTTCGACCG TCTGAAGGCG 
TCTTATGATA CCCTTTGTGT TTTGGACGGA GACAAAGACA GCATTAACTG TGCTAGGCAG 
GAGGCCATCC GCATGGTACG CGTTCTGACT GTGCTCAAGG AGTATATCAA CGAGTGTGAC 
AGCGACTACC ACGAGGAGAG AACCATACTG CCCATGTCCA GAGCTTTCAG GGGAAAGCAC 
ATAACTTTGA TTGTGCGTTT CCCAAACCAA GGTCGCCAAG TAGATGATTT AGATATCTGG 
TCCCACACCA ACGACACGAT TGGCTCGGTG AGGCGGGCGA TTCTAAACAG AATAAAGGCT 
AATGCTGCGC ATACAAAGGT CGAGTTGTTT ATTGGGGGTG AGGTGGTGGA TCCAGCTGAT 
GACAGGAAGT TGATCGGGCA GCTCAGTCTG AAGGACAAAA CGTTGATCAC GGCCAAGCTG 
ACCCAGGTGA GTGCCAACAT GCCCTCAAGT CCAGACAGCT CCTCTGACTC GTCTACTGGC 
TCTCCTGGTA ACCATGGAAA CCACTACAGC GACGGGCCCA ACCCTGAGGT GGAAAGCTGT 
CTGCCTGGCG TGATTATGTC GCTGCATCTG CGGTACATCT CCTTCCTGTG GCAGATCGCT 
GATCTAGGCT GCAGCCTCAA CATGCCTCTA CTTAGAGATG GAGCTCGTGT TCTCATGAAG 
CTTATGCCTC CTGATAACAC CACAGTAGAG AATCTGAGAG CTGTGTGTTT GGACCATGCC 
AAGCTGGGAG AGAACAGCCT GAGTCCCTCA CTGGACTCCT GCTTCTTTGG CCCCTCCCCC 
TCACAAGTGC TCTACCTCAT AGAGGTCGTG TATGCTTTGC TGATGCCAGC CAGTGCCACT 
CTCGGTGAAG ACGCCAGCGA CTTCCAATAC AACTTCCTGA AGAGCGGCGG CCTGCCCTTG 
GTGCTGAGCA TGCTCACCAG AAACAACTTT CTCCCATCGG CAGACATGGA AACTCGAAGG 
GGCGCCTATG TCAACGCACT AAAGATCGCA AAGCTCCTAC TGACCGCTGT GGGTTTTGGA 
CACGTGAAGG CGGTAGCTGA GGCCTGCCAG CCAAACGCCG ACGGAAATAT TCCCGTCTCT 
CCAATTAATC AGGCAACGCA CGACCAAGCG CTGGTTCTTC AGAGCGCCCT GCAAAGCATC 
CCTAACCCGG CCTCAGAGTG CATGCTGCGT AATGTAGCCA TTCGCCTGGC CCAACAGATC 
TCTGACGAGA ACTTTTTCCA GGCGTCGAAG TACATCCCAG ACATTTGTGT AATCCGAGCA 
ATACAGAAAA TCGTGTGGGC ATCAGGCTGT GGTACAGTGC AGCTCGTCTT TAGCTCCAAT 
GAGGAGATCA GCAGGATATA TGAGAAGACA AATGCTGCCA AGGAACCAGA TGGAGAGGAT 
GAGCAGGTGT GCTGCGAGGC CTTGGAGGTC ATGACCCTGT GCTTTGCCCT CATGCCCACG 
GCTCTGGACA CACTAAGCAA AGAGAAGGCG TGGCAGACTT TTATCATAGA CCTGTTACTA 
CACTGCCACA GCAAGTCCGT TCGACAAATG GCTCAGGAGC AGTTTTTCCT CATGGCCACA 
AGATGCTGCA TGGGCCACCG ACCCCTCCTC TTCTTCATCA CCCTGTTATT CACTGTGTTG 
GGGAGCACGG CCAAAGAGAG AGCCAAACAT GCGGGGGACT ACTTCACCTT GCTCAGACAC 
CTGCTAAACT ATGCTTACAA CAGCAACATC AACCTGCCAA ACGCCGAGGT GCTGCTTAAC 
AATGAGATTG ACTGGCTCAA GAGAATACGG GATGAGGTGA AGAGAACAGG AGAGACTGGT 
GTGGAGGAGA CGATCCTGGA GGGCCACCTT GGAGTCACCA AAGAGCTCCT TGCTTTTCAA 
ACACCGGAAA AGAAGTACTA CATTGGCAGC GAGAAGGGAG GAGCCAACCT TATAAAGGAG 
CTGATCGATG ACTTCATATT CCCAGCATCA AATGTTTACC TGCAGTACAT GAAGACGGGA 
GAGTTCCCCA CAGAGCAGGC CATCCCAGTC TGCAGCACTC CCGCCTCCAT CAATGCTGGC 
TTTGAGCTCC TGGTGGCTCT GGCCGTTGGC TGCATGCGCA ATCTGAAACA AATAGTTGAC 
ACCCTGACTG ATATGTACTA CTCAGGCGGG GAGACGTTGA CAGAGTGGGA GTACTTGCCT 
CCAGTTGGGC CACGACCCAA TAAAGGCTTT GTAGGTCTAA AGAACGCCGG TGCCACATGT 
TACATGAACT CTGTCATTCA GCAACTGTAC ATGATCCCTC CCATTCGCAA CGGCATTCTG 
GCTATTGAGG GAACAGGCAC GGATGTGGAC GATGACATGT CGGGGGATGA GAAGCAGGAG 
AATGAGAGTA ACGTCGATCC TCGTGATGAG GTCTTCAGCT ACCATCACCA GTTTGATGAC 
AAACCGTCCA GTAAGTCAGA GGACAGGAAG GAGTACAACA TCGGCGTGCT TCGTCACTTG 
CAGGTCATCT TTGGTCATCT TGCTGCATCC AGGCTGCAGT ACTACGTCCC AAGAGGCTTC 
TGGAAACAGT TTAGATTGTG GGGTGAGCCA GTCAACCTGA GGGAACAGCA CGATGCTCTG 
GAGTTTTTCA ACTCTTTGGT GGACAGTTTG GATGAAGCTC TGAAGGCTCT GGGTCACCCC 
GCCATGCTCA GTAAGGTGCT GGGAGGCTCT TTCGCCGATC AGAAAATCTG TCAAGGGTGC 
CCTCACAGGT ATGAGTGTGA GGAGTCGTTT ACAACACTTA ACGTGGACAT CAGAAACCAC 
CAAAACCTGT TGGACTCCAT GGAGCAGTAT GTCAAAGGAG ATCTGCTGGA AGGAGCAAAT 
GCCTATCACT GTGAGAAGTG TAATAAAAAG GTGGACACCG TGAAGCGTCT GTTGATCAAG 
AAGCTCCCAC CCGTCCTGGC CATTCAGCTG AAGCGCTTTG ACTACGACTG GGAGCGAGAA 
TGTGCCATCA AGTTTAACGA CTACTTTGAG TTCCCCAGGG AGCTGGATAT GGAGCCTTAC 
ACGGTAGCGG GCGTGGCCAA ACTGGAGGGC GACGACGTGA ACCCGGAGAA CCAGGTCATA 
CAGCAGAACG AGCCGTCTGA ATCCACACCG CCGGGCAGCT CCAAATATCG TCTGGTGGGA 
GTGCTGGTCC ACTCAGGCCA GGCCAGTGGC GGACACTACT ATTCCTACAT AATCCAGAGG 
AACGGAGGGG ACGGGGAGAA GAACCGCTGG TATAAGTTCG ACGACGGCGA CGTCACTGAA 
TGCAAGATGG ACGACGAGGA GGAGATGAAG AACCAGTGCT TCGGTGGGGA GTACATGGGC 
GAGGTGTTTG ATCATATGAT GAAAAGGATG TCGTATCGGA GGCAAAAGCG CTGGTGGAAC 
GCTTACATCC TGTTTTACGA GCGAATGGAC TCGTTGGACA AGGACAGCGA TCTCGTCAAA 
TACATCTCGG ACCTGACGAT CTCCACCAAG CCACATCAGG TCAAGATGCC CACGGTCATC 
GAGTGCAGCG TCCGCAAGCA GAACGTCCAG TTCATGCACA ACCGAATGCA ATACAGCCTA 
GAATATTTCC AGTTCATAAA GAAACTTCTG ACCTGTAACA GTGTCTATTT AAACCCTCCT 
CCAGGGCAAG ACCACCTGCT GCCAGAGGCA GAGGAGATCG CGATGATCAG CACTCAGCTG 
GCTGCTAGGT TCCTCTTCAG CACAGGTTTC CACACCAAGA AAGTAGTACG GGGTCCTGCC 
AGTGACTGGT ATGACGCCCT CTGCATCCTG CTGAGACACA GTAAGAATGT TCGTTACTGG 
TTTGCACACA ACGTCCTGTT TGCGTACCCT AACCGCTTCT CCGAGTATTT GCTGGAGTGC 
CCCAGCGCTG AGGTCCGCGG AGCATTTGCC AAGCTCATCG TCTTCATCGC TCACTTCTCC 
CTGCAAGATG GCCCCTGCCC CTCCCCCACC GCCTCACCTG GACCGTCTGC TCAGGGCTGT 
GATAATCTCA GTTTAAGTGA CCACTTGTTG AGAGCTGTTC TAAACCTGCT TAGGAGAGAG 
GTTTCTGAAC ATGGCCGACA TCTACAGCAG TACTTCAACC TCTTTGTCAT GTATGCCAAT 
CTGGGCCTGG CAGAGAAGTC TCAGCTGCTG AAGCTCAACG TTCCCGCCAC TTTCATGCTG 
GTCGCTCTAG ACGAGGGTCC CGGTCCCCCC ATCAAATACC AGTATGCAGA ACTGGGCAAG 
CTCTACACTG TGGTTTCCCA GCTGGTCCGC TGCTGTGAGG TGTCCTCGCG CATGCAATCC 
TCCATCAATG GTAACCCTCC TCTTCCTAAC CCGTATGGGG ATCCCAACCT GACGGCTCCG 
GTGATGTCTG TGCAGCAGCT GGTGGCAGAA ATCCTGTTTG TGAGGACGAG CTACGTCAAG 
AAGATCATCG AGGACTGCAG CAACTCTGAG GAGACCGTGA AGCTCCTTAG ATTCTGCTGC 
TGGGAGAATC CTCAGTTCTC CTCCACTGTG CTCAGTGAAC TGCTTTGGCA GGTGGCATAT 
TCTTACACGT ATGAGCTGAG GCCTTACCTG GACTTGCTGC TACAGATCCT TCTCATCGAA 
GACTCCTGGC AGACGCACAG GATCCACAAT GTCCTAAAGG GCATCCCTGA TGACAGAGAC 
GGGCTGTTTG ACACCATCCA GCGCTCCAAA AACCATTACC AGAAGAGGGC CTACCAGTGC 
ATCAAGTGCA TGGTGGCCCT CTTTAGCAAC TGCTCCGTGG CTTACCAGAT CCTGCAGAGT 
AATGGTGACC TGAAAAGGAA GTGGACGTGG GCGGTGGAGT GGTTGGGGGA TGAGTTGGAG 
AGAAGGCCGT ACACAGGAAA CCCGCAGTAC ACCTACAACA ACTGGTCCCC CCCAGTTCAG 
AGCAACGAGA CCTCCAACGG GTATTTCCTG GAGCGTTCAC ACAGTGCACG TATGACACTT 
GCCAAAGCCT GTGAACTCTG TCCTGAAGAG CTCAAGTGTA CACAGGAAAG CCCAGGGAAG 
GAGCCGGACG AACAGGAAGC ACCTGATGAT CAAGACTCCT CCCCTCCTGA AGACACCTCT 
CTGTACCCAC ATTCTCCTGG AAGCACCCAG TTTCAGCAGA ATAACCACCC CCATGGGCAG 
CCTTACACCG GGCCTGCTGC CCAGCACATG AACAACCCCC AGCGCCCCGG CCCAGCCTCC 
GCTCCGACTC CCGGCCCGGC CCAGACCCCC GCCTCAGGCC CCGGCACCGC CCCTGGTTCC 
GGCCCGCGAG CACAAGAGCA CTGGGAGGGC ACAGAGGAAG TCGCCCCCGC CCCCGCCTCC 
ACCCCAGCAC CTGTCCCCCC GAAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806295.1
CDS	657..8486
Translation	MTATTRGSPVGGNDSQGQGQAPDAQSQPPLPQNQTSSPNSSNENSPVSPPDEQSQVDRPPQLEEEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKCEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMATNPHCKFHIYNGTRPSETVPAGAQLPDDELYARPPDPRSPKGWLVDLINKFGNLNGFQILHDRFMSGQALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPVIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHNPEEEEWLTAERMAEWIQQNHILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTMQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDQALNAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLRKKRPIYIETNLEGQTQRSPHVFYRHDLINQLQHNHALVTLVAENLSAYMETMRQLSKEEQAEFDPQTVRPGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVFLCDREACFKWYSKLMGDEPDLDPDINKDFFENNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQGSDDIASRAIDLLKEIYTNLGPKLQVNQVEIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQEAIRMVRVLTVLKEYINECDSDYHEERTILPMSRAFRGKHITLIVRFPNQGRQVDDLDIWSHTNDTIGSVRRAILNRIKANAAHTKVELFIGGEVVDPADDRKLIGQLSLKDKTLITAKLTQVSANMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHLRYISFLWQIADLGCSLNMPLLRDGARVLMKLMPPDNTTVENLRAVCLDHAKLGENSLSPSLDSCFFGPSPSQVLYLIEVVYALLMPASATLGEDASDFQYNFLKSGGLPLVLSMLTRNNFLPSADMETRRGAYVNALKIAKLLLTAVGFGHVKAVAEACQPNADGNIPVSPINQATHDQALVLQSALQSIPNPASECMLRNVAIRLAQQISDENFFQASKYIPDICVIRAIQKIVWASGCGTVQLVFSSNEEISRIYEKTNAAKEPDGEDEQVCCEALEVMTLCFALMPTALDTLSKEKAWQTFIIDLLLHCHSKSVRQMAQEQFFLMATRCCMGHRPLLFFITLLFTVLGSTAKERAKHAGDYFTLLRHLLNYAYNSNINLPNAEVLLNNEIDWLKRIRDEVKRTGETGVEETILEGHLGVTKELLAFQTPEKKYYIGSEKGGANLIKELIDDFIFPASNVYLQYMKTGEFPTEQAIPVCSTPASINAGFELLVALAVGCMRNLKQIVDTLTDMYYSGGETLTEWEYLPPVGPRPNKGFVGLKNAGATCYMNSVIQQLYMIPPIRNGILAIEGTGTDVDDDMSGDEKQENESNVDPRDEVFSYHHQFDDKPSSKSEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSMEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDDVNPENQVIQQNEPSESTPPGSSKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDEEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDSLDKDSDLVKYISDLTISTKPHQVKMPTVIECSVRKQNVQFMHNRMQYSLEYFQFIKKLLTCNSVYLNPPPGQDHLLPEAEEIAMISTQLAARFLFSTGFHTKKVVRGPASDWYDALCILLRHSKNVRYWFAHNVLFAYPNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPTASPGPSAQGCDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGLAEKSQLLKLNVPATFMLVALDEGPGPPIKYQYAELGKLYTVVSQLVRCCEVSSRMQSSINGNPPLPNPYGDPNLTAPVMSVQQLVAEILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNVLKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCSVAYQILQSNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEELKCTQESPGKEPDEQEAPDDQDSSPPEDTSLYPHSPGSTQFQQNNHPHGQPYTGPAAQHMNNPQRPGPASAPTPGPAQTPASGPGTAPGSGPRAQEHWEGTEEVAPAPASTPAPVPPKE

Target ORF information:

RefSeq Version	XM_023950527.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950527.1

ORF Insert Sequence:

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGAAAG AAAAGGCCAA TTTACATTGA AACGAACTTA 
GAAGGCCAAA CCCAGAGAAG TCCACACGTG TTTTACCGGC ACGACCTGAT CAACCAGCTG 
CAGCATAACC ACGCTCTGGT CACCCTCGTG GCCGAGAACC TCTCTGCTTA CATGGAGACT 
ATGAGGCAGC TATCCAAAGA AGAACAAGCA GAGTTTGATC CTCAGACAGT CAGACCAGGA 
AGCCGCTACA GTCACGTCCA AGAAGTTCAG GAGCGACTCA ACTTCCTGAG GTTTCTTTTA 
AAAGACGGCC AGCTGTGGCT GTGTGCCCCT CAAGCCAAGC AGATCTGGAA GTGTCTGGCT 
GAGAATGCTG TGTTTCTCTG CGATCGCGAA GCTTGCTTCA AATGGTACTC CAAGCTGATG 
GGGGATGAAC CAGACCTGGA TCCGGACATC AATAAGGACT TCTTTGAGAA TAACGTTCTG 
CAGTTGGATC CGTCTCTGCT GACGGAAAAT GGCATGAAGT GCTTTGAGAG GTTCTTCAAG 
GCCGTCAACT GCAGGGAGGG GAAGCTGGTT GCAAAGCGCA GGGCTTACAT GATGGATGAC 
TTGGAGCTAA TAGGCCTGGA CTATCTGTGG AGGGTGGTAA TTCAAGGAAG CGATGACATT 
GCTAGCCGAG CCATAGACCT GCTGAAAGAG ATTTACACCA ACCTTGGTCC CAAACTACAA 
GTCAATCAGG TTGAGATTCA CGAGGATTTC ATCCAGTCCT GCTTCGACCG TCTGAAGGCG 
TCTTATGATA CCCTTTGTGT TTTGGACGGA GACAAAGACA GCATTAACTG TGCTAGGCAG 
GAGGCCATCC GCATGGTACG CGTTCTGACT GTGCTCAAGG AGTATATCAA CGAGTGTGAC 
AGCGACTACC ACGAGGAGAG AACCATACTG CCCATGTCCA GAGCTTTCAG GGGAAAGCAC 
ATAACTTTGA TTGTGCGTTT CCCAAACCAA GGTCGCCAAG TAGATGATTT AGATATCTGG 
TCCCACACCA ACGACACGAT TGGCTCGGTG AGGCGGGCGA TTCTAAACAG AATAAAGGCT 
AATGCTGCGC ATACAAAGGT CGAGTTGTTT ATTGGGGGTG AGGTGGTGGA TCCAGCTGAT 
GACAGGAAGT TGATCGGGCA GCTCAGTCTG AAGGACAAAA CGTTGATCAC GGCCAAGCTG 
ACCCAGGTGA GTGCCAACAT GCCCTCAAGT CCAGACAGCT CCTCTGACTC GTCTACTGGC 
TCTCCTGGTA ACCATGGAAA CCACTACAGC GACGGGCCCA ACCCTGAGGT GGAAAGCTGT 
CTGCCTGGCG TGATTATGTC GCTGCATCTG CGGTACATCT CCTTCCTGTG GCAGATCGCT 
GATCTAGGCT GCAGCCTCAA CATGCCTCTA CTTAGAGATG GAGCTCGTGT TCTCATGAAG 
CTTATGCCTC CTGATAACAC CACAGTAGAG AATCTGAGAG CTGTGTGTTT GGACCATGCC 
AAGCTGGGAG AGAACAGCCT GAGTCCCTCA CTGGACTCCT GCTTCTTTGG CCCCTCCCCC 
TCACAAGTGC TCTACCTCAT AGAGGTCGTG TATGCTTTGC TGATGCCAGC CAGTGCCACT 
CTCGGTGAAG ACGCCAGCGA CTTCCAATAC AACTTCCTGA AGAGCGGCGG CCTGCCCTTG 
GTGCTGAGCA TGCTCACCAG AAACAACTTT CTCCCATCGG CAGACATGGA AACTCGAAGG 
GGCGCCTATG TCAACGCACT AAAGATCGCA AAGCTCCTAC TGACCGCTGT GGGTTTTGGA 
CACGTGAAGG CGGTAGCTGA GGCCTGCCAG CCAAACGCCG ACGGAAATAT TCCCGTCTCT 
CCAATTAATC AGGCAACGCA CGACCAAGCG CTGGTTCTTC AGAGCGCCCT GCAAAGCATC 
CCTAACCCGG CCTCAGAGTG CATGCTGCGT AATGTAGCCA TTCGCCTGGC CCAACAGATC 
TCTGACGAGA ACTTTTTCCA GGCGTCGAAG TACATCCCAG ACATTTGTGT AATCCGAGCA 
ATACAGAAAA TCGTGTGGGC ATCAGGCTGT GGTACAGTGC AGCTCGTCTT TAGCTCCAAT 
GAGGAGATCA GCAGGATATA TGAGAAGACA AATGCTGCCA AGGAACCAGA TGGAGAGGAT 
GAGCAGGTGT GCTGCGAGGC CTTGGAGGTC ATGACCCTGT GCTTTGCCCT CATGCCCACG 
GCTCTGGACA CACTAAGCAA AGAGAAGGCG TGGCAGACTT TTATCATAGA CCTGTTACTA 
CACTGCCACA GCAAGTCCGT TCGACAAATG GCTCAGGAGC AGTTTTTCCT CATGGCCACA 
AGATGCTGCA TGGGCCACCG ACCCCTCCTC TTCTTCATCA CCCTGTTATT CACTGTGTTG 
GGGAGCACGG CCAAAGAGAG AGCCAAACAT GCGGGGGACT ACTTCACCTT GCTCAGACAC 
CTGCTAAACT ATGCTTACAA CAGCAACATC AACCTGCCAA ACGCCGAGGT GCTGCTTAAC 
AATGAGATTG ACTGGCTCAA GAGAATACGG GATGAGGTGA AGAGAACAGG AGAGACTGGT 
GTGGAGGAGA CGATCCTGGA GGGCCACCTT GGAGTCACCA AAGAGCTCCT TGCTTTTCAA 
ACACCGGAAA AGAAGTACTA CATTGGCAGC GAGAAGGGAG GAGCCAACCT TATAAAGGAG 
CTGATCGATG ACTTCATATT CCCAGCATCA AATGTTTACC TGCAGTACAT GAAGACGGGA 
GAGTTCCCCA CAGAGCAGGC CATCCCAGTC TGCAGCACTC CCGCCTCCAT CAATGCTGGC 
TTTGAGCTCC TGGTGGCTCT GGCCGTTGGC TGCATGCGCA ATCTGAAACA AATAGTTGAC 
ACCCTGACTG ATATGTACTA CTCAGGCGGG GAGACGTTGA CAGAGTGGGA GTACTTGCCT 
CCAGTTGGGC CACGACCCAA TAAAGGCTTT GTAGGTCTAA AGAACGCCGG TGCCACATGT 
TACATGAACT CTGTCATTCA GCAACTGTAC ATGATCCCTC CCATTCGCAA CGGCATTCTG 
GCTATTGAGG GAACAGGCAC GGATGTGGAC GATGACATGT CGGGGGATGA GAAGCAGGAG 
AATGAGAGTA ACGTCGATCC TCGTGATGAG GTCTTCAGCT ACCATCACCA GTTTGATGAC 
AAACCGTCCA GTAAGTCAGA GGACAGGAAG GAGTACAACA TCGGCGTGCT TCGTCACTTG 
CAGGTCATCT TTGGTCATCT TGCTGCATCC AGGCTGCAGT ACTACGTCCC AAGAGGCTTC 
TGGAAACAGT TTAGATTGTG GGGTGAGCCA GTCAACCTGA GGGAACAGCA CGATGCTCTG 
GAGTTTTTCA ACTCTTTGGT GGACAGTTTG GATGAAGCTC TGAAGGCTCT GGGTCACCCC 
GCCATGCTCA GTAAGGTGCT GGGAGGCTCT TTCGCCGATC AGAAAATCTG TCAAGGGTGC 
CCTCACAGGT ATGAGTGTGA GGAGTCGTTT ACAACACTTA ACGTGGACAT CAGAAACCAC 
CAAAACCTGT TGGACTCCAT GGAGCAGTAT GTCAAAGGAG ATCTGCTGGA AGGAGCAAAT 
GCCTATCACT GTGAGAAGTG TAATAAAAAG GTGGACACCG TGAAGCGTCT GTTGATCAAG 
AAGCTCCCAC CCGTCCTGGC CATTCAGCTG AAGCGCTTTG ACTACGACTG GGAGCGAGAA 
TGTGCCATCA AGTTTAACGA CTACTTTGAG TTCCCCAGGG AGCTGGATAT GGAGCCTTAC 
ACGGTAGCGG GCGTGGCCAA ACTGGAGGGC GACGACGTGA ACCCGGAGAA CCAGGTCATA 
CAGCAGAACG AGCCGTCTGA ATCCACACCG CCGGGCAGCT CCAAATATCG TCTGGTGGGA 
GTGCTGGTCC ACTCAGGCCA GGCCAGTGGC GGACACTACT ATTCCTACAT AATCCAGAGG 
AACGGAGGGG ACGGGGAGAA GAACCGCTGG TATAAGTTCG ACGACGGCGA CGTCACTGAA 
TGCAAGATGG ACGACGAGGA GGAGATGAAG AACCAGTGCT TCGGTGGGGA GTACATGGGC 
GAGGTGTTTG ATCATATGAT GAAAAGGATG TCGTATCGGA GGCAAAAGCG CTGGTGGAAC 
GCTTACATCC TGTTTTACGA GCGAATGGAC TCGTTGGACA AGGACAGCGA TCTCGTCAAA 
TACATCTCGG ACCTGACGAT CTCCACCAAG CCACATCAGG TCAAGATGCC CACGGTCATC 
GAGTGCAGCG TCCGCAAGCA GAACGTCCAG TTCATGCACA ACCGAATGCA ATACAGCCTA 
GAATATTTCC AGTTCATAAA GAAACTTCTG ACCTGTAACA GTGTCTATTT AAACCCTCCT 
CCAGGGCAAG ACCACCTGCT GCCAGAGGCA GAGGAGATCG CGATGATCAG CACTCAGCTG 
GCTGCTAGGT TCCTCTTCAG CACAGGTTTC CACACCAAGA AAGTAGTACG GGGTCCTGCC 
AGTGACTGGT ATGACGCCCT CTGCATCCTG CTGAGACACA GTAAGAATGT TCGTTACTGG 
TTTGCACACA ACGTCCTGTT TGCGTACCCT AACCGCTTCT CCGAGTATTT GCTGGAGTGC 
CCCAGCGCTG AGGTCCGCGG AGCATTTGCC AAGCTCATCG TCTTCATCGC TCACTTCTCC 
CTGCAAGATG GCCCCTGCCC CTCCCCCACC GCCTCACCTG GACCGTCTGC TCAGGGCTGT 
GATAATCTCA GTTTAAGTGA CCACTTGTTG AGAGCTGTTC TAAACCTGCT TAGGAGAGAG 
GTTTCTGAAC ATGGCCGACA TCTACAGCAG TACTTCAACC TCTTTGTCAT GTATGCCAAT 
CTGGGCCTGG CAGAGAAGTC TCAGCTGCTG AAGCTCAACG TTCCCGCCAC TTTCATGCTG 
GTCGCTCTAG ACGAGGGTCC CGGTCCCCCC ATCAAATACC AGTATGCAGA ACTGGGCAAG 
CTCTACACTG TGGTTTCCCA GCTGGTCCGC TGCTGTGAGG TGTCCTCGCG CATGCAATCC 
TCCATCAATG GTAACCCTCC TCTTCCTAAC CCGTATGGGG ATCCCAACCT GACGGCTCCG 
GTGATGTCTG TGCAGCAGCT GGTGGCAGAA ATCCTGTTTG TGAGGACGAG CTACGTCAAG 
AAGATCATCG AGGACTGCAG CAACTCTGAG GAGACCGTGA AGCTCCTTAG ATTCTGCTGC 
TGGGAGAATC CTCAGTTCTC CTCCACTGTG CTCAGTGAAC TGCTTTGGCA GGTGGCATAT 
TCTTACACGT ATGAGCTGAG GCCTTACCTG GACTTGCTGC TACAGATCCT TCTCATCGAA 
GACTCCTGGC AGACGCACAG GATCCACAAT GTCCTAAAGG GCATCCCTGA TGACAGAGAC 
GGGCTGTTTG ACACCATCCA GCGCTCCAAA AACCATTACC AGAAGAGGGC CTACCAGTGC 
ATCAAGTGCA TGGTGGCCCT CTTTAGCAAC TGCTCCGTGG CTTACCAGAT CCTGCAGAGT 
AATGGTGACC TGAAAAGGAA GTGGACGTGG GCGGTGGAGT GGTTGGGGGA TGAGTTGGAG 
AGAAGGCCGT ACACAGGAAA CCCGCAGTAC ACCTACAACA ACTGGTCCCC CCCAGTTCAG 
AGCAACGAGA CCTCCAACGG GTATTTCCTG GAGCGTTCAC ACAGTGCACG TATGACACTT 
GCCAAAGCCT GTGAACTCTG TCCTGAAGAG CTCAAGTGTA CACAGGAAAG CCCAGGGAAG 
GAGCCGGACG AACAGGAAGC ACCTGATGAT CAAGACTCCT CCCCTCCTGA AGACACCTCT 
CTGTACCCAC ATTCTCCTGG AAGCACCCAG TTTCAGCAGA ATAACCACCC CCATGGGCAG 
CCTTACACCG GGCCTGCTGC CCAGCACATG AACAACCCCC AGCGCCCCGG CCCAGCCTCC 
GCTCCGACTC CCGGCCCGGC CCAGACCCCC GCCTCAGGCC CCGGCACCGC CCCTGGTTCC 
GGCCCGCGAG CACAAGAGCA CTGGGAGGGC ACAGAGGAAG TCGCCCCCGC CCCCGCCTCC 
ACCCCAGCAC CTGTCCCCCC GAAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp9x ( XM_023950528.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806296.1 Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X4, mRNA.

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CloneID	OJa113029
Clone ID Related Accession (Same CDS sequence)	XM_023950528.1
Accession Version	XM_023950528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGAAAG AAAAGGCCAA TTTACATTGA AACGAACTTA 
GAAGGCCAAA CCCAGAGAAG TCCACACGTG TTTTACCGGC ACGACCTGAT CAACCAGCTG 
CAGCATAACC ACGCTCTGGT CACCCTCGTG GCCGAGAACC TCTCTGCTTA CATGGAGACT 
ATGAGGCAGC TATCCAAAGA AGAACAAGCA GAGTTTGATC CTCAGACAGT CAGACCAGGA 
AGCCGCTACA GTCACGTCCA AGAAGTTCAG GAGCGACTCA ACTTCCTGAG GTTTCTTTTA 
AAAGACGGCC AGCTGTGGCT GTGTGCCCCT CAAGCCAAGC AGATCTGGAA GTGTCTGGCT 
GAGAATGCTG TGTTTCTCTG CGATCGCGAA GCTTGCTTCA AATGGTACTC CAAGCTGATG 
GGGGATGAAC CAGACCTGGA TCCGGACATC AATAAGGACT TCTTTGAGAA TAACGTTCTG 
CAGTTGGATC CGTCTCTGCT GACGGAAAAT GGCATGAAGT GCTTTGAGAG GTTCTTCAAG 
GCCGTCAACT GCAGGGAGGG GAAGCTGGTT GCAAAGCGCA GGGCTTACAT GATGGATGAC 
TTGGAGCTAA TAGGCCTGGA CTATCTGTGG AGGGTGGTAA TTCAAGGAAG CGATGACATT 
GCTAGCCGAG CCATAGACCT GCTGAAAGAG ATTTACACCA ACCTTGGTCC CAAACTACAA 
GTCAATCAGG TTGAGATTCA CGAGGATTTC ATCCAGTCCT GCTTCGACCG TCTGAAGGCG 
TCTTATGATA CCCTTTGTGT TTTGGACGGA GACAAAGACA GCATTAACTG TGCTAGGCAG 
GAGGCCATCC GCATGGTACG CGTTCTGACT GTGCTCAAGG AGTATATCAA CGAGTGTGAC 
AGCGACTACC ACGAGGAGAG AACCATACTG CCCATGTCCA GAGCTTTCAG GGGAAAGCAC 
ATAACTTTGA TTGTGCGTTT CCCAAACCAA GGTCGCCAAG TAGATGATTT AGATATCTGG 
TCCCACACCA ACGACACGAT TGGCTCGGTG AGGCGGGCGA TTCTAAACAG AATAAAGGCT 
AATGCTGCGC ATACAAAGGT CGAGTTGTTT ATTGGGGGTG AGGTGGTGGA TCCAGCTGAT 
GACAGGAAGT TGATCGGGCA GCTCAGTCTG AAGGACAAAA CGTTGATCAC GGCCAAGCTG 
ACCCAGGTGA GTGCCAACAT GCCCTCAAGT CCAGACAGCT CCTCTGACTC GTCTACTGGC 
TCTCCTGGTA ACCATGGAAA CCACTACAGC GACGGGCCCA ACCCTGAGGT GGAAAGCTGT 
CTGCCTGGCG TGATTATGTC GCTGCATCTG CGGTACATCT CCTTCCTGTG GCAGATCGCT 
GATCTAGGCT GCAGCCTCAA CATGCCTCTA CTTAGAGATG GAGCTCGTGT TCTCATGAAG 
CTTATGCCTC CTGATAACAC CACAGTAGAG AATCTGAGAG CTGTGTGTTT GGACCATGCC 
AAGCTGGGAG AGAACAGCCT GAGTCCCTCA CTGGACTCCT GCTTCTTTGG CCCCTCCCCC 
TCACAAGTGC TCTACCTCAT AGAGGTCGTG TATGCTTTGC TGATGCCAGC CAGTGCCACT 
CTCGGTGAAG ACGCCAGCGA CTTCCAATAC AACTTCCTGA AGAGCGGCGG CCTGCCCTTG 
GTGCTGAGCA TGCTCACCAG AAACAACTTT CTCCCATCGG CAGACATGGA AACTCGAAGG 
GGCGCCTATG TCAACGCACT AAAGATCGCA AAGCTCCTAC TGACCGCTGT GGGTTTTGGA 
CACGTGAAGG CGGTAGCTGA GGCCTGCCAG CCAAACGCCG ACGGAAATAT TCCCGTCTCT 
CCAATTAATC AGGCAACGCA CGACCAAGCG CTGGTTCTTC AGAGCGCCCT GCAAAGCATC 
CCTAACCCGG CCTCAGAGTG CATGCTGCGT AATGTAGCCA TTCGCCTGGC CCAACAGATC 
TCTGACGAGA ACTCTATTTG CCATCAGAAC TTTTTCCAGG CGTCGAAGTA CATCCCAGAC 
ATTTGTGTAA TCCGAGCAAT ACAGAAAATC GTGTGGGCAT CAGGCTGTGG TACAGTGCAG 
CTCGTCTTTA GCTCCAATGA GGAGATCAGC AGGATATATG AGAAGACAAA TGCTGCCAAG 
GAACCAGATG GAGAGGATGA GCAGGTGTGC TGCGAGGCCT TGGAGGTCAT GACCCTGTGC 
TTTGCCCTCA TGCCCACGGC TCTGGACACA CTAAGCAAAG AGAAGGCGTG GCAGACTTTT 
ATCATAGACC TGTTACTACA CTGCCACAGC AAGTCCGTTC GACAAATGGC TCAGGAGCAG 
TTTTTCCTCA TGGCCACAAG ATGCTGCATG GGCCACCGAC CCCTCCTCTT CTTCATCACC 
CTGTTATTCA CTGTGTTGGG GAGCACGGCC AAAGAGAGAG CCAAACATGC GGGGGACTAC 
TTCACCTTGC TCAGACACCT GCTAAACTAT GCTTACAACA GCAACATCAA CCTGCCAAAC 
GCCGAGGTGC TGCTTAACAA TGAGATTGAC TGGCTCAAGA GAATACGGGA TGAGGTGAAG 
AGAACAGGAG AGACTGGTGT GGAGGAGACG ATCCTGGAGG GCCACCTTGG AGTCACCAAA 
GAGCTCCTTG CTTTTCAAAC ACCGGAAAAG AAGTACTACA TTGGCAGCGA GAAGGGAGGA 
GCCAACCTTA TAAAGGAGCT GATCGATGAC TTCATATTCC CAGCATCAAA TGTTTACCTG 
CAGTACATGA AGACGGGAGA GTTCCCCACA GAGCAGGCCA TCCCAGTCTG CAGCACTCCC 
GCCTCCATCA ATGCTGGCTT TGAGCTCCTG GTGGCTCTGG CCGTTGGCTG CATGCGCAAT 
CTGAAACAAA TAGTTGACAC CCTGACTGAT ATGTACTACT CAGGCGGGGA GACGTTGACA 
GAGTGGGAGT ACTTGCCTCC AGTTGGGCCA CGACCCAATA AAGGCTTTGT AGGTCTAAAG 
AACGCCGGTG CCACATGTTA CATGAACTCT GTCATTCAGC AACTGTACAT GATCCCTCCC 
ATTCGCAACG GCATTCTGGC TATTGAGGGA ACAGGCACGG ATGTGGACGA TGACATGTCG 
GGGGATGAGA AGCAGGAGAA TGAGAGTAAC GTCGATCCTC GTGATGAGGT CTTCAGCTAC 
CATCACCAGT TTGATGACAA ACCGTCCAGT AAGTCAGAGG ACAGGAAGGA GTACAACATC 
GGCGTGCTTC GTCACTTGCA GGTCATCTTT GGTCATCTTG CTGCATCCAG GCTGCAGTAC 
TACGTCCCAA GAGGCTTCTG GAAACAGTTT AGATTGTGGG GTGAGCCAGT CAACCTGAGG 
GAACAGCACG ATGCTCTGGA GTTTTTCAAC TCTTTGGTGG ACAGTTTGGA TGAAGCTCTG 
AAGGCTCTGG GTCACCCCGC CATGCTCAGT AAGGTGCTGG GAGGCTCTTT CGCCGATCAG 
AAAATCTGTC AAGGGTGCCC TCACAGGTAT GAGTGTGAGG AGTCGTTTAC AACACTTAAC 
GTGGACATCA GAAACCACCA AAACCTGTTG GACTCCATGG AGCAGTATGT CAAAGGAGAT 
CTGCTGGAAG GAGCAAATGC CTATCACTGT GAGAAGTGTA ATAAAAAGGT GGACACCGTG 
AAGCGTCTGT TGATCAAGAA GCTCCCACCC GTCCTGGCCA TTCAGCTGAA GCGCTTTGAC 
TACGACTGGG AGCGAGAATG TGCCATCAAG TTTAACGACT ACTTTGAGTT CCCCAGGGAG 
CTGGATATGG AGCCTTACAC GGTAGCGGGC GTGGCCAAAC TGGAGGGCGA CGACGTGAAC 
CCGGAGAACC AGGTCATACA GCAGAACGAG CCGTCTGAAT CCACACCGCC GGGCAGCTCC 
AAATATCGTC TGGTGGGAGT GCTGGTCCAC TCAGGCCAGG CCAGTGGCGG ACACTACTAT 
TCCTACATAA TCCAGAGGAA CGGAGGGGAC GGGGAGAAGA ACCGCTGGTA TAAGTTCGAC 
GACGGCGACG TCACTGAATG CAAGATGGAC GACGAGGAGG AGATGAAGAA CCAGTGCTTC 
GGTGGGGAGT ACATGGGCGA GGTGTTTGAT CATATGATGA AAAGGATGTC GTATCGGAGG 
CAAAAGCGCT GGTGGAACGC TTACATCCTG TTTTACGAGC GAATGGACTC GTTGGACAAG 
GACAGCGATC TCGTCAAATA CATCTCGGAC CTGACGATCT CCACCAAGCC ACATCAGGTC 
AAGATGCCCA CGGTCATCGA GTGCAGCGTC CGCAAGCAGA ACGTCCAGTT CATGCACAAC 
CGAATGCAAT ACAGCCTAGA ATATTTCCAG TTCATAAAGA AACTTCTGAC CTGTAACAGT 
GTCTATTTAA ACCCTCCTCC AGGGCAAGAC CACCTGCTGC CAGAGGCAGA GGAGATCGCG 
ATGATCAGCA CTCAGCTGGC TGCTAGGTTC CTCTTCAGCA CAGGTTTCCA CACCAAGAAA 
GTAGTACGGG GTCCTGCCAG TGACTGGTAT GACGCCCTCT GCATCCTGCT GAGACACAGT 
AAGAATGTTC GTTACTGGTT TGCACACAAC GTCCTGTTTG CGTACCCTAA CCGCTTCTCC 
GAGTATTTGC TGGAGTGCCC CAGCGCTGAG GTCCGCGGAG CATTTGCCAA GCTCATCGTC 
TTCATCGCTC ACTTCTCCCT GCAAGATGGC CCCTGCCCCT CCCCCACCGC CTCACCTGGA 
CCGTCTGCTC AGGGCTGTGA TAATCTCAGT TTAAGTGACC ACTTGTTGAG AGCTGTTCTA 
AACCTGCTTA GGAGAGAGGT TTCTGAACAT GGCCGACATC TACAGCAGTA CTTCAACCTC 
TTTGTCATGT ATGCCAATCT GGGCCTGGCA GAGAAGTCTC AGCTGCTGAA GCTCAACGTT 
CCCGCCACTT TCATGCTGGT CGCTCTAGAC GAGGGTCCCG GTCCCCCCAT CAAATACCAG 
TATGCAGAAC TGGGCAAGCT CTACACTGTG GTTTCCCAGC TGGTCCGCTG CTGTGAGGTG 
TCCTCGCGCA TGCAATCCTC CATCAATGGT AACCCTCCTC TTCCTAACCC GTATGGGGAT 
CCCAACCTGA CGGCTCCGGT GATGTCTGTG CAGCAGCTGG TGGCAGAAAT CCTGTTTGTG 
AGGACGAGCT ACGTCAAGAA GATCATCGAG GACTGCAGCA ACTCTGAGGA GACCGTGAAG 
CTCCTTAGAT TCTGCTGCTG GGAGAATCCT CAGTTCTCCT CCACTGTGCT CAGTGAACTG 
CTTTGGCAGG TGGCATATTC TTACACGTAT GAGCTGAGGC CTTACCTGGA CTTGCTGCTA 
CAGATCCTTC TCATCGAAGA CTCCTGGCAG ACGCACAGGA TCCACAATGT CCTAAAGGGC 
ATCCCTGATG ACAGAGACGG GCTGTTTGAC ACCATCCAGC GCTCCAAAAA CCATTACCAG 
AAGAGGGCCT ACCAGTGCAT CAAGTGCATG GTGGCCCTCT TTAGCAACTG CTCCGTGGCT 
TACCAGATCC TGCAGAGTAA TGGTGACCTG AAAAGGAAGT GGACGTGGGC GGTGGAGTGG 
TTGGGGGATG AGTTGGAGAG AAGGCCGTAC ACAGGAAACC CGCAGTACAC CTACAACAAC 
TGGTCCCCCC CAGTTCAGAG CAACGAGACC TCCAACGGGT ATTTCCTGGA GCGTTCACAC 
AGTGCACGTA TGACACTTGC CAAAGCCTGT GAACTCTGTC CTGAAGAGGA GCCGGACGAA 
CAGGAAGCAC CTGATGATCA AGACTCCTCC CCTCCTGAAG ACACCTCTCT GTACCCACAT 
TCTCCTGGAA GCACCCAGTT TCAGCAGAAT AACCACCCCC ATGGGCAGCC TTACACCGGG 
CCTGCTGCCC AGCACATGAA CAACCCCCAG CGCCCCGGCC CAGCCTCCGC TCCGACTCCC 
GGCCCGGCCC AGACCCCCGC CTCAGGCCCC GGCACCGCCC CTGGTTCCGG CCCGCGAGCA 
CAAGAGCACT GGGAGGGCAC AGAGGAAGTC GCCCCCGCCC CCGCCTCCAC CCCAGCACCT 
GTCCCCCCGA AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806296.1
CDS	659..8476
Translation	MTATTRGSPVGGNDSQGQGQAPDAQSQPPLPQNQTSSPNSSNENSPVSPPDEQSQVDRPPQLEEEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKCEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMATNPHCKFHIYNGTRPSETVPAGAQLPDDELYARPPDPRSPKGWLVDLINKFGNLNGFQILHDRFMSGQALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPVIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHNPEEEEWLTAERMAEWIQQNHILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTMQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDQALNAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLRKKRPIYIETNLEGQTQRSPHVFYRHDLINQLQHNHALVTLVAENLSAYMETMRQLSKEEQAEFDPQTVRPGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVFLCDREACFKWYSKLMGDEPDLDPDINKDFFENNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQGSDDIASRAIDLLKEIYTNLGPKLQVNQVEIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQEAIRMVRVLTVLKEYINECDSDYHEERTILPMSRAFRGKHITLIVRFPNQGRQVDDLDIWSHTNDTIGSVRRAILNRIKANAAHTKVELFIGGEVVDPADDRKLIGQLSLKDKTLITAKLTQVSANMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHLRYISFLWQIADLGCSLNMPLLRDGARVLMKLMPPDNTTVENLRAVCLDHAKLGENSLSPSLDSCFFGPSPSQVLYLIEVVYALLMPASATLGEDASDFQYNFLKSGGLPLVLSMLTRNNFLPSADMETRRGAYVNALKIAKLLLTAVGFGHVKAVAEACQPNADGNIPVSPINQATHDQALVLQSALQSIPNPASECMLRNVAIRLAQQISDENSICHQNFFQASKYIPDICVIRAIQKIVWASGCGTVQLVFSSNEEISRIYEKTNAAKEPDGEDEQVCCEALEVMTLCFALMPTALDTLSKEKAWQTFIIDLLLHCHSKSVRQMAQEQFFLMATRCCMGHRPLLFFITLLFTVLGSTAKERAKHAGDYFTLLRHLLNYAYNSNINLPNAEVLLNNEIDWLKRIRDEVKRTGETGVEETILEGHLGVTKELLAFQTPEKKYYIGSEKGGANLIKELIDDFIFPASNVYLQYMKTGEFPTEQAIPVCSTPASINAGFELLVALAVGCMRNLKQIVDTLTDMYYSGGETLTEWEYLPPVGPRPNKGFVGLKNAGATCYMNSVIQQLYMIPPIRNGILAIEGTGTDVDDDMSGDEKQENESNVDPRDEVFSYHHQFDDKPSSKSEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSMEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDDVNPENQVIQQNEPSESTPPGSSKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDEEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDSLDKDSDLVKYISDLTISTKPHQVKMPTVIECSVRKQNVQFMHNRMQYSLEYFQFIKKLLTCNSVYLNPPPGQDHLLPEAEEIAMISTQLAARFLFSTGFHTKKVVRGPASDWYDALCILLRHSKNVRYWFAHNVLFAYPNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPTASPGPSAQGCDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGLAEKSQLLKLNVPATFMLVALDEGPGPPIKYQYAELGKLYTVVSQLVRCCEVSSRMQSSINGNPPLPNPYGDPNLTAPVMSVQQLVAEILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNVLKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCSVAYQILQSNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEEEPDEQEAPDDQDSSPPEDTSLYPHSPGSTQFQQNNHPHGQPYTGPAAQHMNNPQRPGPASAPTPGPAQTPASGPGTAPGSGPRAQEHWEGTEEVAPAPASTPAPVPPKE

Target ORF information:

RefSeq Version	XM_023950528.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950528.1

ORF Insert Sequence:

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGAAAG AAAAGGCCAA TTTACATTGA AACGAACTTA 
GAAGGCCAAA CCCAGAGAAG TCCACACGTG TTTTACCGGC ACGACCTGAT CAACCAGCTG 
CAGCATAACC ACGCTCTGGT CACCCTCGTG GCCGAGAACC TCTCTGCTTA CATGGAGACT 
ATGAGGCAGC TATCCAAAGA AGAACAAGCA GAGTTTGATC CTCAGACAGT CAGACCAGGA 
AGCCGCTACA GTCACGTCCA AGAAGTTCAG GAGCGACTCA ACTTCCTGAG GTTTCTTTTA 
AAAGACGGCC AGCTGTGGCT GTGTGCCCCT CAAGCCAAGC AGATCTGGAA GTGTCTGGCT 
GAGAATGCTG TGTTTCTCTG CGATCGCGAA GCTTGCTTCA AATGGTACTC CAAGCTGATG 
GGGGATGAAC CAGACCTGGA TCCGGACATC AATAAGGACT TCTTTGAGAA TAACGTTCTG 
CAGTTGGATC CGTCTCTGCT GACGGAAAAT GGCATGAAGT GCTTTGAGAG GTTCTTCAAG 
GCCGTCAACT GCAGGGAGGG GAAGCTGGTT GCAAAGCGCA GGGCTTACAT GATGGATGAC 
TTGGAGCTAA TAGGCCTGGA CTATCTGTGG AGGGTGGTAA TTCAAGGAAG CGATGACATT 
GCTAGCCGAG CCATAGACCT GCTGAAAGAG ATTTACACCA ACCTTGGTCC CAAACTACAA 
GTCAATCAGG TTGAGATTCA CGAGGATTTC ATCCAGTCCT GCTTCGACCG TCTGAAGGCG 
TCTTATGATA CCCTTTGTGT TTTGGACGGA GACAAAGACA GCATTAACTG TGCTAGGCAG 
GAGGCCATCC GCATGGTACG CGTTCTGACT GTGCTCAAGG AGTATATCAA CGAGTGTGAC 
AGCGACTACC ACGAGGAGAG AACCATACTG CCCATGTCCA GAGCTTTCAG GGGAAAGCAC 
ATAACTTTGA TTGTGCGTTT CCCAAACCAA GGTCGCCAAG TAGATGATTT AGATATCTGG 
TCCCACACCA ACGACACGAT TGGCTCGGTG AGGCGGGCGA TTCTAAACAG AATAAAGGCT 
AATGCTGCGC ATACAAAGGT CGAGTTGTTT ATTGGGGGTG AGGTGGTGGA TCCAGCTGAT 
GACAGGAAGT TGATCGGGCA GCTCAGTCTG AAGGACAAAA CGTTGATCAC GGCCAAGCTG 
ACCCAGGTGA GTGCCAACAT GCCCTCAAGT CCAGACAGCT CCTCTGACTC GTCTACTGGC 
TCTCCTGGTA ACCATGGAAA CCACTACAGC GACGGGCCCA ACCCTGAGGT GGAAAGCTGT 
CTGCCTGGCG TGATTATGTC GCTGCATCTG CGGTACATCT CCTTCCTGTG GCAGATCGCT 
GATCTAGGCT GCAGCCTCAA CATGCCTCTA CTTAGAGATG GAGCTCGTGT TCTCATGAAG 
CTTATGCCTC CTGATAACAC CACAGTAGAG AATCTGAGAG CTGTGTGTTT GGACCATGCC 
AAGCTGGGAG AGAACAGCCT GAGTCCCTCA CTGGACTCCT GCTTCTTTGG CCCCTCCCCC 
TCACAAGTGC TCTACCTCAT AGAGGTCGTG TATGCTTTGC TGATGCCAGC CAGTGCCACT 
CTCGGTGAAG ACGCCAGCGA CTTCCAATAC AACTTCCTGA AGAGCGGCGG CCTGCCCTTG 
GTGCTGAGCA TGCTCACCAG AAACAACTTT CTCCCATCGG CAGACATGGA AACTCGAAGG 
GGCGCCTATG TCAACGCACT AAAGATCGCA AAGCTCCTAC TGACCGCTGT GGGTTTTGGA 
CACGTGAAGG CGGTAGCTGA GGCCTGCCAG CCAAACGCCG ACGGAAATAT TCCCGTCTCT 
CCAATTAATC AGGCAACGCA CGACCAAGCG CTGGTTCTTC AGAGCGCCCT GCAAAGCATC 
CCTAACCCGG CCTCAGAGTG CATGCTGCGT AATGTAGCCA TTCGCCTGGC CCAACAGATC 
TCTGACGAGA ACTCTATTTG CCATCAGAAC TTTTTCCAGG CGTCGAAGTA CATCCCAGAC 
ATTTGTGTAA TCCGAGCAAT ACAGAAAATC GTGTGGGCAT CAGGCTGTGG TACAGTGCAG 
CTCGTCTTTA GCTCCAATGA GGAGATCAGC AGGATATATG AGAAGACAAA TGCTGCCAAG 
GAACCAGATG GAGAGGATGA GCAGGTGTGC TGCGAGGCCT TGGAGGTCAT GACCCTGTGC 
TTTGCCCTCA TGCCCACGGC TCTGGACACA CTAAGCAAAG AGAAGGCGTG GCAGACTTTT 
ATCATAGACC TGTTACTACA CTGCCACAGC AAGTCCGTTC GACAAATGGC TCAGGAGCAG 
TTTTTCCTCA TGGCCACAAG ATGCTGCATG GGCCACCGAC CCCTCCTCTT CTTCATCACC 
CTGTTATTCA CTGTGTTGGG GAGCACGGCC AAAGAGAGAG CCAAACATGC GGGGGACTAC 
TTCACCTTGC TCAGACACCT GCTAAACTAT GCTTACAACA GCAACATCAA CCTGCCAAAC 
GCCGAGGTGC TGCTTAACAA TGAGATTGAC TGGCTCAAGA GAATACGGGA TGAGGTGAAG 
AGAACAGGAG AGACTGGTGT GGAGGAGACG ATCCTGGAGG GCCACCTTGG AGTCACCAAA 
GAGCTCCTTG CTTTTCAAAC ACCGGAAAAG AAGTACTACA TTGGCAGCGA GAAGGGAGGA 
GCCAACCTTA TAAAGGAGCT GATCGATGAC TTCATATTCC CAGCATCAAA TGTTTACCTG 
CAGTACATGA AGACGGGAGA GTTCCCCACA GAGCAGGCCA TCCCAGTCTG CAGCACTCCC 
GCCTCCATCA ATGCTGGCTT TGAGCTCCTG GTGGCTCTGG CCGTTGGCTG CATGCGCAAT 
CTGAAACAAA TAGTTGACAC CCTGACTGAT ATGTACTACT CAGGCGGGGA GACGTTGACA 
GAGTGGGAGT ACTTGCCTCC AGTTGGGCCA CGACCCAATA AAGGCTTTGT AGGTCTAAAG 
AACGCCGGTG CCACATGTTA CATGAACTCT GTCATTCAGC AACTGTACAT GATCCCTCCC 
ATTCGCAACG GCATTCTGGC TATTGAGGGA ACAGGCACGG ATGTGGACGA TGACATGTCG 
GGGGATGAGA AGCAGGAGAA TGAGAGTAAC GTCGATCCTC GTGATGAGGT CTTCAGCTAC 
CATCACCAGT TTGATGACAA ACCGTCCAGT AAGTCAGAGG ACAGGAAGGA GTACAACATC 
GGCGTGCTTC GTCACTTGCA GGTCATCTTT GGTCATCTTG CTGCATCCAG GCTGCAGTAC 
TACGTCCCAA GAGGCTTCTG GAAACAGTTT AGATTGTGGG GTGAGCCAGT CAACCTGAGG 
GAACAGCACG ATGCTCTGGA GTTTTTCAAC TCTTTGGTGG ACAGTTTGGA TGAAGCTCTG 
AAGGCTCTGG GTCACCCCGC CATGCTCAGT AAGGTGCTGG GAGGCTCTTT CGCCGATCAG 
AAAATCTGTC AAGGGTGCCC TCACAGGTAT GAGTGTGAGG AGTCGTTTAC AACACTTAAC 
GTGGACATCA GAAACCACCA AAACCTGTTG GACTCCATGG AGCAGTATGT CAAAGGAGAT 
CTGCTGGAAG GAGCAAATGC CTATCACTGT GAGAAGTGTA ATAAAAAGGT GGACACCGTG 
AAGCGTCTGT TGATCAAGAA GCTCCCACCC GTCCTGGCCA TTCAGCTGAA GCGCTTTGAC 
TACGACTGGG AGCGAGAATG TGCCATCAAG TTTAACGACT ACTTTGAGTT CCCCAGGGAG 
CTGGATATGG AGCCTTACAC GGTAGCGGGC GTGGCCAAAC TGGAGGGCGA CGACGTGAAC 
CCGGAGAACC AGGTCATACA GCAGAACGAG CCGTCTGAAT CCACACCGCC GGGCAGCTCC 
AAATATCGTC TGGTGGGAGT GCTGGTCCAC TCAGGCCAGG CCAGTGGCGG ACACTACTAT 
TCCTACATAA TCCAGAGGAA CGGAGGGGAC GGGGAGAAGA ACCGCTGGTA TAAGTTCGAC 
GACGGCGACG TCACTGAATG CAAGATGGAC GACGAGGAGG AGATGAAGAA CCAGTGCTTC 
GGTGGGGAGT ACATGGGCGA GGTGTTTGAT CATATGATGA AAAGGATGTC GTATCGGAGG 
CAAAAGCGCT GGTGGAACGC TTACATCCTG TTTTACGAGC GAATGGACTC GTTGGACAAG 
GACAGCGATC TCGTCAAATA CATCTCGGAC CTGACGATCT CCACCAAGCC ACATCAGGTC 
AAGATGCCCA CGGTCATCGA GTGCAGCGTC CGCAAGCAGA ACGTCCAGTT CATGCACAAC 
CGAATGCAAT ACAGCCTAGA ATATTTCCAG TTCATAAAGA AACTTCTGAC CTGTAACAGT 
GTCTATTTAA ACCCTCCTCC AGGGCAAGAC CACCTGCTGC CAGAGGCAGA GGAGATCGCG 
ATGATCAGCA CTCAGCTGGC TGCTAGGTTC CTCTTCAGCA CAGGTTTCCA CACCAAGAAA 
GTAGTACGGG GTCCTGCCAG TGACTGGTAT GACGCCCTCT GCATCCTGCT GAGACACAGT 
AAGAATGTTC GTTACTGGTT TGCACACAAC GTCCTGTTTG CGTACCCTAA CCGCTTCTCC 
GAGTATTTGC TGGAGTGCCC CAGCGCTGAG GTCCGCGGAG CATTTGCCAA GCTCATCGTC 
TTCATCGCTC ACTTCTCCCT GCAAGATGGC CCCTGCCCCT CCCCCACCGC CTCACCTGGA 
CCGTCTGCTC AGGGCTGTGA TAATCTCAGT TTAAGTGACC ACTTGTTGAG AGCTGTTCTA 
AACCTGCTTA GGAGAGAGGT TTCTGAACAT GGCCGACATC TACAGCAGTA CTTCAACCTC 
TTTGTCATGT ATGCCAATCT GGGCCTGGCA GAGAAGTCTC AGCTGCTGAA GCTCAACGTT 
CCCGCCACTT TCATGCTGGT CGCTCTAGAC GAGGGTCCCG GTCCCCCCAT CAAATACCAG 
TATGCAGAAC TGGGCAAGCT CTACACTGTG GTTTCCCAGC TGGTCCGCTG CTGTGAGGTG 
TCCTCGCGCA TGCAATCCTC CATCAATGGT AACCCTCCTC TTCCTAACCC GTATGGGGAT 
CCCAACCTGA CGGCTCCGGT GATGTCTGTG CAGCAGCTGG TGGCAGAAAT CCTGTTTGTG 
AGGACGAGCT ACGTCAAGAA GATCATCGAG GACTGCAGCA ACTCTGAGGA GACCGTGAAG 
CTCCTTAGAT TCTGCTGCTG GGAGAATCCT CAGTTCTCCT CCACTGTGCT CAGTGAACTG 
CTTTGGCAGG TGGCATATTC TTACACGTAT GAGCTGAGGC CTTACCTGGA CTTGCTGCTA 
CAGATCCTTC TCATCGAAGA CTCCTGGCAG ACGCACAGGA TCCACAATGT CCTAAAGGGC 
ATCCCTGATG ACAGAGACGG GCTGTTTGAC ACCATCCAGC GCTCCAAAAA CCATTACCAG 
AAGAGGGCCT ACCAGTGCAT CAAGTGCATG GTGGCCCTCT TTAGCAACTG CTCCGTGGCT 
TACCAGATCC TGCAGAGTAA TGGTGACCTG AAAAGGAAGT GGACGTGGGC GGTGGAGTGG 
TTGGGGGATG AGTTGGAGAG AAGGCCGTAC ACAGGAAACC CGCAGTACAC CTACAACAAC 
TGGTCCCCCC CAGTTCAGAG CAACGAGACC TCCAACGGGT ATTTCCTGGA GCGTTCACAC 
AGTGCACGTA TGACACTTGC CAAAGCCTGT GAACTCTGTC CTGAAGAGGA GCCGGACGAA 
CAGGAAGCAC CTGATGATCA AGACTCCTCC CCTCCTGAAG ACACCTCTCT GTACCCACAT 
TCTCCTGGAA GCACCCAGTT TCAGCAGAAT AACCACCCCC ATGGGCAGCC TTACACCGGG 
CCTGCTGCCC AGCACATGAA CAACCCCCAG CGCCCCGGCC CAGCCTCCGC TCCGACTCCC 
GGCCCGGCCC AGACCCCCGC CTCAGGCCCC GGCACCGCCC CTGGTTCCGG CCCGCGAGCA 
CAAGAGCACT GGGAGGGCAC AGAGGAAGTC GCCCCCGCCC CCGCCTCCAC CCCAGCACCT 
GTCCCCCCGA AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp9x ( XM_023950529.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806297.1 Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X5, mRNA.

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CloneID	OJa113030
Clone ID Related Accession (Same CDS sequence)	XM_023950529.1
Accession Version	XM_023950529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGCCAA ACCCAGAGAA GTCCACACGT GTTTTACCGG 
CACGACCTGA TCAACCAGCT GCAGCATAAC CACGCTCTGG TCACCCTCGT GGCCGAGAAC 
CTCTCTGCTT ACATGGAGAC TATGAGGCAG CTATCCAAAG AAGAACAAGC AGAGTTTGAT 
CCTCAGACAG TCAGACCAGG AAGCCGCTAC AGTCACGTCC AAGAAGTTCA GGAGCGACTC 
AACTTCCTGA GGTTTCTTTT AAAAGACGGC CAGCTGTGGC TGTGTGCCCC TCAAGCCAAG 
CAGATCTGGA AGTGTCTGGC TGAGAATGCT GTGTTTCTCT GCGATCGCGA AGCTTGCTTC 
AAATGGTACT CCAAGCTGAT GGGGGATGAA CCAGACCTGG ATCCGGACAT CAATAAGGAC 
TTCTTTGAGA ATAACGTTCT GCAGTTGGAT CCGTCTCTGC TGACGGAAAA TGGCATGAAG 
TGCTTTGAGA GGTTCTTCAA GGCCGTCAAC TGCAGGGAGG GGAAGCTGGT TGCAAAGCGC 
AGGGCTTACA TGATGGATGA CTTGGAGCTA ATAGGCCTGG ACTATCTGTG GAGGGTGGTA 
ATTCAAGGAA GCGATGACAT TGCTAGCCGA GCCATAGACC TGCTGAAAGA GATTTACACC 
AACCTTGGTC CCAAACTACA AGTCAATCAG GTTGAGATTC ACGAGGATTT CATCCAGTCC 
TGCTTCGACC GTCTGAAGGC GTCTTATGAT ACCCTTTGTG TTTTGGACGG AGACAAAGAC 
AGCATTAACT GTGCTAGGCA GGAGGCCATC CGCATGGTAC GCGTTCTGAC TGTGCTCAAG 
GAGTATATCA ACGAGTGTGA CAGCGACTAC CACGAGGAGA GAACCATACT GCCCATGTCC 
AGAGCTTTCA GGGGAAAGCA CATAACTTTG ATTGTGCGTT TCCCAAACCA AGGTCGCCAA 
GTAGATGATT TAGATATCTG GTCCCACACC AACGACACGA TTGGCTCGGT GAGGCGGGCG 
ATTCTAAACA GAATAAAGGC TAATGCTGCG CATACAAAGG TCGAGTTGTT TATTGGGGGT 
GAGGTGGTGG ATCCAGCTGA TGACAGGAAG TTGATCGGGC AGCTCAGTCT GAAGGACAAA 
ACGTTGATCA CGGCCAAGCT GACCCAGGTG AGTGCCAACA TGCCCTCAAG TCCAGACAGC 
TCCTCTGACT CGTCTACTGG CTCTCCTGGT AACCATGGAA ACCACTACAG CGACGGGCCC 
AACCCTGAGG TGGAAAGCTG TCTGCCTGGC GTGATTATGT CGCTGCATCT GCGGTACATC 
TCCTTCCTGT GGCAGATCGC TGATCTAGGC TGCAGCCTCA ACATGCCTCT ACTTAGAGAT 
GGAGCTCGTG TTCTCATGAA GCTTATGCCT CCTGATAACA CCACAGTAGA GAATCTGAGA 
GCTGTGTGTT TGGACCATGC CAAGCTGGGA GAGAACAGCC TGAGTCCCTC ACTGGACTCC 
TGCTTCTTTG GCCCCTCCCC CTCACAAGTG CTCTACCTCA TAGAGGTCGT GTATGCTTTG 
CTGATGCCAG CCAGTGCCAC TCTCGGTGAA GACGCCAGCG ACTTCCAATA CAACTTCCTG 
AAGAGCGGCG GCCTGCCCTT GGTGCTGAGC ATGCTCACCA GAAACAACTT TCTCCCATCG 
GCAGACATGG AAACTCGAAG GGGCGCCTAT GTCAACGCAC TAAAGATCGC AAAGCTCCTA 
CTGACCGCTG TGGGTTTTGG ACACGTGAAG GCGGTAGCTG AGGCCTGCCA GCCAAACGCC 
GACGGAAATA TTCCCGTCTC TCCAATTAAT CAGGCAACGC ACGACCAAGC GCTGGTTCTT 
CAGAGCGCCC TGCAAAGCAT CCCTAACCCG GCCTCAGAGT GCATGCTGCG TAATGTAGCC 
ATTCGCCTGG CCCAACAGAT CTCTGACGAG AACTCTATTT GCCATCAGAA CTTTTTCCAG 
GCGTCGAAGT ACATCCCAGA CATTTGTGTA ATCCGAGCAA TACAGAAAAT CGTGTGGGCA 
TCAGGCTGTG GTACAGTGCA GCTCGTCTTT AGCTCCAATG AGGAGATCAG CAGGATATAT 
GAGAAGACAA ATGCTGCCAA GGAACCAGAT GGAGAGGATG AGCAGGTGTG CTGCGAGGCC 
TTGGAGGTCA TGACCCTGTG CTTTGCCCTC ATGCCCACGG CTCTGGACAC ACTAAGCAAA 
GAGAAGGCGT GGCAGACTTT TATCATAGAC CTGTTACTAC ACTGCCACAG CAAGTCCGTT 
CGACAAATGG CTCAGGAGCA GTTTTTCCTC ATGGCCACAA GATGCTGCAT GGGCCACCGA 
CCCCTCCTCT TCTTCATCAC CCTGTTATTC ACTGTGTTGG GGAGCACGGC CAAAGAGAGA 
GCCAAACATG CGGGGGACTA CTTCACCTTG CTCAGACACC TGCTAAACTA TGCTTACAAC 
AGCAACATCA ACCTGCCAAA CGCCGAGGTG CTGCTTAACA ATGAGATTGA CTGGCTCAAG 
AGAATACGGG ATGAGGTGAA GAGAACAGGA GAGACTGGTG TGGAGGAGAC GATCCTGGAG 
GGCCACCTTG GAGTCACCAA AGAGCTCCTT GCTTTTCAAA CACCGGAAAA GAAGTACTAC 
ATTGGCAGCG AGAAGGGAGG AGCCAACCTT ATAAAGGAGC TGATCGATGA CTTCATATTC 
CCAGCATCAA ATGTTTACCT GCAGTACATG AAGACGGGAG AGTTCCCCAC AGAGCAGGCC 
ATCCCAGTCT GCAGCACTCC CGCCTCCATC AATGCTGGCT TTGAGCTCCT GGTGGCTCTG 
GCCGTTGGCT GCATGCGCAA TCTGAAACAA ATAGTTGACA CCCTGACTGA TATGTACTAC 
TCAGGCGGGG AGACGTTGAC AGAGTGGGAG TACTTGCCTC CAGTTGGGCC ACGACCCAAT 
AAAGGCTTTG TAGGTCTAAA GAACGCCGGT GCCACATGTT ACATGAACTC TGTCATTCAG 
CAACTGTACA TGATCCCTCC CATTCGCAAC GGCATTCTGG CTATTGAGGG AACAGGCACG 
GATGTGGACG ATGACATGTC GGGGGATGAG AAGCAGGAGA ATGAGAGTAA CGTCGATCCT 
CGTGATGAGG TCTTCAGCTA CCATCACCAG TTTGATGACA AACCGTCCAG TAAGTCAGAG 
GACAGGAAGG AGTACAACAT CGGCGTGCTT CGTCACTTGC AGGTCATCTT TGGTCATCTT 
GCTGCATCCA GGCTGCAGTA CTACGTCCCA AGAGGCTTCT GGAAACAGTT TAGATTGTGG 
GGTGAGCCAG TCAACCTGAG GGAACAGCAC GATGCTCTGG AGTTTTTCAA CTCTTTGGTG 
GACAGTTTGG ATGAAGCTCT GAAGGCTCTG GGTCACCCCG CCATGCTCAG TAAGGTGCTG 
GGAGGCTCTT TCGCCGATCA GAAAATCTGT CAAGGGTGCC CTCACAGGTA TGAGTGTGAG 
GAGTCGTTTA CAACACTTAA CGTGGACATC AGAAACCACC AAAACCTGTT GGACTCCATG 
GAGCAGTATG TCAAAGGAGA TCTGCTGGAA GGAGCAAATG CCTATCACTG TGAGAAGTGT 
AATAAAAAGG TGGACACCGT GAAGCGTCTG TTGATCAAGA AGCTCCCACC CGTCCTGGCC 
ATTCAGCTGA AGCGCTTTGA CTACGACTGG GAGCGAGAAT GTGCCATCAA GTTTAACGAC 
TACTTTGAGT TCCCCAGGGA GCTGGATATG GAGCCTTACA CGGTAGCGGG CGTGGCCAAA 
CTGGAGGGCG ACGACGTGAA CCCGGAGAAC CAGGTCATAC AGCAGAACGA GCCGTCTGAA 
TCCACACCGC CGGGCAGCTC CAAATATCGT CTGGTGGGAG TGCTGGTCCA CTCAGGCCAG 
GCCAGTGGCG GACACTACTA TTCCTACATA ATCCAGAGGA ACGGAGGGGA CGGGGAGAAG 
AACCGCTGGT ATAAGTTCGA CGACGGCGAC GTCACTGAAT GCAAGATGGA CGACGAGGAG 
GAGATGAAGA ACCAGTGCTT CGGTGGGGAG TACATGGGCG AGGTGTTTGA TCATATGATG 
AAAAGGATGT CGTATCGGAG GCAAAAGCGC TGGTGGAACG CTTACATCCT GTTTTACGAG 
CGAATGGACT CGTTGGACAA GGACAGCGAT CTCGTCAAAT ACATCTCGGA CCTGACGATC 
TCCACCAAGC CACATCAGGT CAAGATGCCC ACGGTCATCG AGTGCAGCGT CCGCAAGCAG 
AACGTCCAGT TCATGCACAA CCGAATGCAA TACAGCCTAG AATATTTCCA GTTCATAAAG 
AAACTTCTGA CCTGTAACAG TGTCTATTTA AACCCTCCTC CAGGGCAAGA CCACCTGCTG 
CCAGAGGCAG AGGAGATCGC GATGATCAGC ACTCAGCTGG CTGCTAGGTT CCTCTTCAGC 
ACAGGTTTCC ACACCAAGAA AGTAGTACGG GGTCCTGCCA GTGACTGGTA TGACGCCCTC 
TGCATCCTGC TGAGACACAG TAAGAATGTT CGTTACTGGT TTGCACACAA CGTCCTGTTT 
GCGTACCCTA ACCGCTTCTC CGAGTATTTG CTGGAGTGCC CCAGCGCTGA GGTCCGCGGA 
GCATTTGCCA AGCTCATCGT CTTCATCGCT CACTTCTCCC TGCAAGATGG CCCCTGCCCC 
TCCCCCACCG CCTCACCTGG ACCGTCTGCT CAGGGCTGTG ATAATCTCAG TTTAAGTGAC 
CACTTGTTGA GAGCTGTTCT AAACCTGCTT AGGAGAGAGG TTTCTGAACA TGGCCGACAT 
CTACAGCAGT ACTTCAACCT CTTTGTCATG TATGCCAATC TGGGCCTGGC AGAGAAGTCT 
CAGCTGCTGA AGCTCAACGT TCCCGCCACT TTCATGCTGG TCGCTCTAGA CGAGGGTCCC 
GGTCCCCCCA TCAAATACCA GTATGCAGAA CTGGGCAAGC TCTACACTGT GGTTTCCCAG 
CTGGTCCGCT GCTGTGAGGT GTCCTCGCGC ATGCAATCCT CCATCAATGG TAACCCTCCT 
CTTCCTAACC CGTATGGGGA TCCCAACCTG ACGGCTCCGG TGATGTCTGT GCAGCAGCTG 
GTGGCAGAAA TCCTGTTTGT GAGGACGAGC TACGTCAAGA AGATCATCGA GGACTGCAGC 
AACTCTGAGG AGACCGTGAA GCTCCTTAGA TTCTGCTGCT GGGAGAATCC TCAGTTCTCC 
TCCACTGTGC TCAGTGAACT GCTTTGGCAG GTGGCATATT CTTACACGTA TGAGCTGAGG 
CCTTACCTGG ACTTGCTGCT ACAGATCCTT CTCATCGAAG ACTCCTGGCA GACGCACAGG 
ATCCACAATG TCCTAAAGGG CATCCCTGAT GACAGAGACG GGCTGTTTGA CACCATCCAG 
CGCTCCAAAA ACCATTACCA GAAGAGGGCC TACCAGTGCA TCAAGTGCAT GGTGGCCCTC 
TTTAGCAACT GCTCCGTGGC TTACCAGATC CTGCAGAGTA ATGGTGACCT GAAAAGGAAG 
TGGACGTGGG CGGTGGAGTG GTTGGGGGAT GAGTTGGAGA GAAGGCCGTA CACAGGAAAC 
CCGCAGTACA CCTACAACAA CTGGTCCCCC CCAGTTCAGA GCAACGAGAC CTCCAACGGG 
TATTTCCTGG AGCGTTCACA CAGTGCACGT ATGACACTTG CCAAAGCCTG TGAACTCTGT 
CCTGAAGAGC TCAAGTGTAC ACAGGAAAGC CCAGGGAAGG AGCCGGACGA ACAGGAAGCA 
CCTGATGATC AAGACTCCTC CCCTCCTGAA GACACCTCTC TGTACCCACA TTCTCCTGGA 
AGCACCCAGT TTCAGCAGAA TAACCACCCC CATGGGCAGC CTTACACCGG GCCTGCTGCC 
CAGCACATGA ACAACCCCCA GCGCCCCGGC CCAGCCTCCG CTCCGACTCC CGGCCCGGCC 
CAGACCCCCG CCTCAGGCCC CGGCACCGCC CCTGGTTCCG GCCCGCGAGC ACAAGAGCAC 
TGGGAGGGCA CAGAGGAAGT CGCCCCCGCC CCCGCCTCCA CCCCAGCACC TGTCCCCCCG 
AAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806297.1
CDS	657..8465
Translation	MTATTRGSPVGGNDSQGQGQAPDAQSQPPLPQNQTSSPNSSNENSPVSPPDEQSQVDRPPQLEEEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKCEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMATNPHCKFHIYNGTRPSETVPAGAQLPDDELYARPPDPRSPKGWLVDLINKFGNLNGFQILHDRFMSGQALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPVIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHNPEEEEWLTAERMAEWIQQNHILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTMQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDQALNAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLSAYMETMRQLSKEEQAEFDPQTVRPGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVFLCDREACFKWYSKLMGDEPDLDPDINKDFFENNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQGSDDIASRAIDLLKEIYTNLGPKLQVNQVEIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQEAIRMVRVLTVLKEYINECDSDYHEERTILPMSRAFRGKHITLIVRFPNQGRQVDDLDIWSHTNDTIGSVRRAILNRIKANAAHTKVELFIGGEVVDPADDRKLIGQLSLKDKTLITAKLTQVSANMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHLRYISFLWQIADLGCSLNMPLLRDGARVLMKLMPPDNTTVENLRAVCLDHAKLGENSLSPSLDSCFFGPSPSQVLYLIEVVYALLMPASATLGEDASDFQYNFLKSGGLPLVLSMLTRNNFLPSADMETRRGAYVNALKIAKLLLTAVGFGHVKAVAEACQPNADGNIPVSPINQATHDQALVLQSALQSIPNPASECMLRNVAIRLAQQISDENSICHQNFFQASKYIPDICVIRAIQKIVWASGCGTVQLVFSSNEEISRIYEKTNAAKEPDGEDEQVCCEALEVMTLCFALMPTALDTLSKEKAWQTFIIDLLLHCHSKSVRQMAQEQFFLMATRCCMGHRPLLFFITLLFTVLGSTAKERAKHAGDYFTLLRHLLNYAYNSNINLPNAEVLLNNEIDWLKRIRDEVKRTGETGVEETILEGHLGVTKELLAFQTPEKKYYIGSEKGGANLIKELIDDFIFPASNVYLQYMKTGEFPTEQAIPVCSTPASINAGFELLVALAVGCMRNLKQIVDTLTDMYYSGGETLTEWEYLPPVGPRPNKGFVGLKNAGATCYMNSVIQQLYMIPPIRNGILAIEGTGTDVDDDMSGDEKQENESNVDPRDEVFSYHHQFDDKPSSKSEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSMEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDDVNPENQVIQQNEPSESTPPGSSKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDEEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDSLDKDSDLVKYISDLTISTKPHQVKMPTVIECSVRKQNVQFMHNRMQYSLEYFQFIKKLLTCNSVYLNPPPGQDHLLPEAEEIAMISTQLAARFLFSTGFHTKKVVRGPASDWYDALCILLRHSKNVRYWFAHNVLFAYPNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPTASPGPSAQGCDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGLAEKSQLLKLNVPATFMLVALDEGPGPPIKYQYAELGKLYTVVSQLVRCCEVSSRMQSSINGNPPLPNPYGDPNLTAPVMSVQQLVAEILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNVLKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCSVAYQILQSNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEELKCTQESPGKEPDEQEAPDDQDSSPPEDTSLYPHSPGSTQFQQNNHPHGQPYTGPAAQHMNNPQRPGPASAPTPGPAQTPASGPGTAPGSGPRAQEHWEGTEEVAPAPASTPAPVPPKE

Target ORF information:

RefSeq Version	XM_023950529.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950529.1

ORF Insert Sequence:

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGCCAA ACCCAGAGAA GTCCACACGT GTTTTACCGG 
CACGACCTGA TCAACCAGCT GCAGCATAAC CACGCTCTGG TCACCCTCGT GGCCGAGAAC 
CTCTCTGCTT ACATGGAGAC TATGAGGCAG CTATCCAAAG AAGAACAAGC AGAGTTTGAT 
CCTCAGACAG TCAGACCAGG AAGCCGCTAC AGTCACGTCC AAGAAGTTCA GGAGCGACTC 
AACTTCCTGA GGTTTCTTTT AAAAGACGGC CAGCTGTGGC TGTGTGCCCC TCAAGCCAAG 
CAGATCTGGA AGTGTCTGGC TGAGAATGCT GTGTTTCTCT GCGATCGCGA AGCTTGCTTC 
AAATGGTACT CCAAGCTGAT GGGGGATGAA CCAGACCTGG ATCCGGACAT CAATAAGGAC 
TTCTTTGAGA ATAACGTTCT GCAGTTGGAT CCGTCTCTGC TGACGGAAAA TGGCATGAAG 
TGCTTTGAGA GGTTCTTCAA GGCCGTCAAC TGCAGGGAGG GGAAGCTGGT TGCAAAGCGC 
AGGGCTTACA TGATGGATGA CTTGGAGCTA ATAGGCCTGG ACTATCTGTG GAGGGTGGTA 
ATTCAAGGAA GCGATGACAT TGCTAGCCGA GCCATAGACC TGCTGAAAGA GATTTACACC 
AACCTTGGTC CCAAACTACA AGTCAATCAG GTTGAGATTC ACGAGGATTT CATCCAGTCC 
TGCTTCGACC GTCTGAAGGC GTCTTATGAT ACCCTTTGTG TTTTGGACGG AGACAAAGAC 
AGCATTAACT GTGCTAGGCA GGAGGCCATC CGCATGGTAC GCGTTCTGAC TGTGCTCAAG 
GAGTATATCA ACGAGTGTGA CAGCGACTAC CACGAGGAGA GAACCATACT GCCCATGTCC 
AGAGCTTTCA GGGGAAAGCA CATAACTTTG ATTGTGCGTT TCCCAAACCA AGGTCGCCAA 
GTAGATGATT TAGATATCTG GTCCCACACC AACGACACGA TTGGCTCGGT GAGGCGGGCG 
ATTCTAAACA GAATAAAGGC TAATGCTGCG CATACAAAGG TCGAGTTGTT TATTGGGGGT 
GAGGTGGTGG ATCCAGCTGA TGACAGGAAG TTGATCGGGC AGCTCAGTCT GAAGGACAAA 
ACGTTGATCA CGGCCAAGCT GACCCAGGTG AGTGCCAACA TGCCCTCAAG TCCAGACAGC 
TCCTCTGACT CGTCTACTGG CTCTCCTGGT AACCATGGAA ACCACTACAG CGACGGGCCC 
AACCCTGAGG TGGAAAGCTG TCTGCCTGGC GTGATTATGT CGCTGCATCT GCGGTACATC 
TCCTTCCTGT GGCAGATCGC TGATCTAGGC TGCAGCCTCA ACATGCCTCT ACTTAGAGAT 
GGAGCTCGTG TTCTCATGAA GCTTATGCCT CCTGATAACA CCACAGTAGA GAATCTGAGA 
GCTGTGTGTT TGGACCATGC CAAGCTGGGA GAGAACAGCC TGAGTCCCTC ACTGGACTCC 
TGCTTCTTTG GCCCCTCCCC CTCACAAGTG CTCTACCTCA TAGAGGTCGT GTATGCTTTG 
CTGATGCCAG CCAGTGCCAC TCTCGGTGAA GACGCCAGCG ACTTCCAATA CAACTTCCTG 
AAGAGCGGCG GCCTGCCCTT GGTGCTGAGC ATGCTCACCA GAAACAACTT TCTCCCATCG 
GCAGACATGG AAACTCGAAG GGGCGCCTAT GTCAACGCAC TAAAGATCGC AAAGCTCCTA 
CTGACCGCTG TGGGTTTTGG ACACGTGAAG GCGGTAGCTG AGGCCTGCCA GCCAAACGCC 
GACGGAAATA TTCCCGTCTC TCCAATTAAT CAGGCAACGC ACGACCAAGC GCTGGTTCTT 
CAGAGCGCCC TGCAAAGCAT CCCTAACCCG GCCTCAGAGT GCATGCTGCG TAATGTAGCC 
ATTCGCCTGG CCCAACAGAT CTCTGACGAG AACTCTATTT GCCATCAGAA CTTTTTCCAG 
GCGTCGAAGT ACATCCCAGA CATTTGTGTA ATCCGAGCAA TACAGAAAAT CGTGTGGGCA 
TCAGGCTGTG GTACAGTGCA GCTCGTCTTT AGCTCCAATG AGGAGATCAG CAGGATATAT 
GAGAAGACAA ATGCTGCCAA GGAACCAGAT GGAGAGGATG AGCAGGTGTG CTGCGAGGCC 
TTGGAGGTCA TGACCCTGTG CTTTGCCCTC ATGCCCACGG CTCTGGACAC ACTAAGCAAA 
GAGAAGGCGT GGCAGACTTT TATCATAGAC CTGTTACTAC ACTGCCACAG CAAGTCCGTT 
CGACAAATGG CTCAGGAGCA GTTTTTCCTC ATGGCCACAA GATGCTGCAT GGGCCACCGA 
CCCCTCCTCT TCTTCATCAC CCTGTTATTC ACTGTGTTGG GGAGCACGGC CAAAGAGAGA 
GCCAAACATG CGGGGGACTA CTTCACCTTG CTCAGACACC TGCTAAACTA TGCTTACAAC 
AGCAACATCA ACCTGCCAAA CGCCGAGGTG CTGCTTAACA ATGAGATTGA CTGGCTCAAG 
AGAATACGGG ATGAGGTGAA GAGAACAGGA GAGACTGGTG TGGAGGAGAC GATCCTGGAG 
GGCCACCTTG GAGTCACCAA AGAGCTCCTT GCTTTTCAAA CACCGGAAAA GAAGTACTAC 
ATTGGCAGCG AGAAGGGAGG AGCCAACCTT ATAAAGGAGC TGATCGATGA CTTCATATTC 
CCAGCATCAA ATGTTTACCT GCAGTACATG AAGACGGGAG AGTTCCCCAC AGAGCAGGCC 
ATCCCAGTCT GCAGCACTCC CGCCTCCATC AATGCTGGCT TTGAGCTCCT GGTGGCTCTG 
GCCGTTGGCT GCATGCGCAA TCTGAAACAA ATAGTTGACA CCCTGACTGA TATGTACTAC 
TCAGGCGGGG AGACGTTGAC AGAGTGGGAG TACTTGCCTC CAGTTGGGCC ACGACCCAAT 
AAAGGCTTTG TAGGTCTAAA GAACGCCGGT GCCACATGTT ACATGAACTC TGTCATTCAG 
CAACTGTACA TGATCCCTCC CATTCGCAAC GGCATTCTGG CTATTGAGGG AACAGGCACG 
GATGTGGACG ATGACATGTC GGGGGATGAG AAGCAGGAGA ATGAGAGTAA CGTCGATCCT 
CGTGATGAGG TCTTCAGCTA CCATCACCAG TTTGATGACA AACCGTCCAG TAAGTCAGAG 
GACAGGAAGG AGTACAACAT CGGCGTGCTT CGTCACTTGC AGGTCATCTT TGGTCATCTT 
GCTGCATCCA GGCTGCAGTA CTACGTCCCA AGAGGCTTCT GGAAACAGTT TAGATTGTGG 
GGTGAGCCAG TCAACCTGAG GGAACAGCAC GATGCTCTGG AGTTTTTCAA CTCTTTGGTG 
GACAGTTTGG ATGAAGCTCT GAAGGCTCTG GGTCACCCCG CCATGCTCAG TAAGGTGCTG 
GGAGGCTCTT TCGCCGATCA GAAAATCTGT CAAGGGTGCC CTCACAGGTA TGAGTGTGAG 
GAGTCGTTTA CAACACTTAA CGTGGACATC AGAAACCACC AAAACCTGTT GGACTCCATG 
GAGCAGTATG TCAAAGGAGA TCTGCTGGAA GGAGCAAATG CCTATCACTG TGAGAAGTGT 
AATAAAAAGG TGGACACCGT GAAGCGTCTG TTGATCAAGA AGCTCCCACC CGTCCTGGCC 
ATTCAGCTGA AGCGCTTTGA CTACGACTGG GAGCGAGAAT GTGCCATCAA GTTTAACGAC 
TACTTTGAGT TCCCCAGGGA GCTGGATATG GAGCCTTACA CGGTAGCGGG CGTGGCCAAA 
CTGGAGGGCG ACGACGTGAA CCCGGAGAAC CAGGTCATAC AGCAGAACGA GCCGTCTGAA 
TCCACACCGC CGGGCAGCTC CAAATATCGT CTGGTGGGAG TGCTGGTCCA CTCAGGCCAG 
GCCAGTGGCG GACACTACTA TTCCTACATA ATCCAGAGGA ACGGAGGGGA CGGGGAGAAG 
AACCGCTGGT ATAAGTTCGA CGACGGCGAC GTCACTGAAT GCAAGATGGA CGACGAGGAG 
GAGATGAAGA ACCAGTGCTT CGGTGGGGAG TACATGGGCG AGGTGTTTGA TCATATGATG 
AAAAGGATGT CGTATCGGAG GCAAAAGCGC TGGTGGAACG CTTACATCCT GTTTTACGAG 
CGAATGGACT CGTTGGACAA GGACAGCGAT CTCGTCAAAT ACATCTCGGA CCTGACGATC 
TCCACCAAGC CACATCAGGT CAAGATGCCC ACGGTCATCG AGTGCAGCGT CCGCAAGCAG 
AACGTCCAGT TCATGCACAA CCGAATGCAA TACAGCCTAG AATATTTCCA GTTCATAAAG 
AAACTTCTGA CCTGTAACAG TGTCTATTTA AACCCTCCTC CAGGGCAAGA CCACCTGCTG 
CCAGAGGCAG AGGAGATCGC GATGATCAGC ACTCAGCTGG CTGCTAGGTT CCTCTTCAGC 
ACAGGTTTCC ACACCAAGAA AGTAGTACGG GGTCCTGCCA GTGACTGGTA TGACGCCCTC 
TGCATCCTGC TGAGACACAG TAAGAATGTT CGTTACTGGT TTGCACACAA CGTCCTGTTT 
GCGTACCCTA ACCGCTTCTC CGAGTATTTG CTGGAGTGCC CCAGCGCTGA GGTCCGCGGA 
GCATTTGCCA AGCTCATCGT CTTCATCGCT CACTTCTCCC TGCAAGATGG CCCCTGCCCC 
TCCCCCACCG CCTCACCTGG ACCGTCTGCT CAGGGCTGTG ATAATCTCAG TTTAAGTGAC 
CACTTGTTGA GAGCTGTTCT AAACCTGCTT AGGAGAGAGG TTTCTGAACA TGGCCGACAT 
CTACAGCAGT ACTTCAACCT CTTTGTCATG TATGCCAATC TGGGCCTGGC AGAGAAGTCT 
CAGCTGCTGA AGCTCAACGT TCCCGCCACT TTCATGCTGG TCGCTCTAGA CGAGGGTCCC 
GGTCCCCCCA TCAAATACCA GTATGCAGAA CTGGGCAAGC TCTACACTGT GGTTTCCCAG 
CTGGTCCGCT GCTGTGAGGT GTCCTCGCGC ATGCAATCCT CCATCAATGG TAACCCTCCT 
CTTCCTAACC CGTATGGGGA TCCCAACCTG ACGGCTCCGG TGATGTCTGT GCAGCAGCTG 
GTGGCAGAAA TCCTGTTTGT GAGGACGAGC TACGTCAAGA AGATCATCGA GGACTGCAGC 
AACTCTGAGG AGACCGTGAA GCTCCTTAGA TTCTGCTGCT GGGAGAATCC TCAGTTCTCC 
TCCACTGTGC TCAGTGAACT GCTTTGGCAG GTGGCATATT CTTACACGTA TGAGCTGAGG 
CCTTACCTGG ACTTGCTGCT ACAGATCCTT CTCATCGAAG ACTCCTGGCA GACGCACAGG 
ATCCACAATG TCCTAAAGGG CATCCCTGAT GACAGAGACG GGCTGTTTGA CACCATCCAG 
CGCTCCAAAA ACCATTACCA GAAGAGGGCC TACCAGTGCA TCAAGTGCAT GGTGGCCCTC 
TTTAGCAACT GCTCCGTGGC TTACCAGATC CTGCAGAGTA ATGGTGACCT GAAAAGGAAG 
TGGACGTGGG CGGTGGAGTG GTTGGGGGAT GAGTTGGAGA GAAGGCCGTA CACAGGAAAC 
CCGCAGTACA CCTACAACAA CTGGTCCCCC CCAGTTCAGA GCAACGAGAC CTCCAACGGG 
TATTTCCTGG AGCGTTCACA CAGTGCACGT ATGACACTTG CCAAAGCCTG TGAACTCTGT 
CCTGAAGAGC TCAAGTGTAC ACAGGAAAGC CCAGGGAAGG AGCCGGACGA ACAGGAAGCA 
CCTGATGATC AAGACTCCTC CCCTCCTGAA GACACCTCTC TGTACCCACA TTCTCCTGGA 
AGCACCCAGT TTCAGCAGAA TAACCACCCC CATGGGCAGC CTTACACCGG GCCTGCTGCC 
CAGCACATGA ACAACCCCCA GCGCCCCGGC CCAGCCTCCG CTCCGACTCC CGGCCCGGCC 
CAGACCCCCG CCTCAGGCCC CGGCACCGCC CCTGGTTCCG GCCCGCGAGC ACAAGAGCAC 
TGGGAGGGCA CAGAGGAAGT CGCCCCCGCC CCCGCCTCCA CCCCAGCACC TGTCCCCCCG 
AAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp9x ( XM_023950530.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806298.1 Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X6, mRNA.

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CloneID	OJa113031
Clone ID Related Accession (Same CDS sequence)	XM_023950530.1
Accession Version	XM_023950530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGCCAA ACCCAGAGAA GTCCACACGT GTTTTACCGG 
CACGACCTGA TCAACCAGCT GCAGCATAAC CACGCTCTGG TCACCCTCGT GGCCGAGAAC 
CTCTCTGCTT ACATGGAGAC TATGAGGCAG CTATCCAAAG AACAAGCAGA GTTTGATCCT 
CAGACAGTCA GACCAGGAAG CCGCTACAGT CACGTCCAAG AAGTTCAGGA GCGACTCAAC 
TTCCTGAGGT TTCTTTTAAA AGACGGCCAG CTGTGGCTGT GTGCCCCTCA AGCCAAGCAG 
ATCTGGAAGT GTCTGGCTGA GAATGCTGTG TTTCTCTGCG ATCGCGAAGC TTGCTTCAAA 
TGGTACTCCA AGCTGATGGG GGATGAACCA GACCTGGATC CGGACATCAA TAAGGACTTC 
TTTGAGAATA ACGTTCTGCA GTTGGATCCG TCTCTGCTGA CGGAAAATGG CATGAAGTGC 
TTTGAGAGGT TCTTCAAGGC CGTCAACTGC AGGGAGGGGA AGCTGGTTGC AAAGCGCAGG 
GCTTACATGA TGGATGACTT GGAGCTAATA GGCCTGGACT ATCTGTGGAG GGTGGTAATT 
CAAGGAAGCG ATGACATTGC TAGCCGAGCC ATAGACCTGC TGAAAGAGAT TTACACCAAC 
CTTGGTCCCA AACTACAAGT CAATCAGGTT GAGATTCACG AGGATTTCAT CCAGTCCTGC 
TTCGACCGTC TGAAGGCGTC TTATGATACC CTTTGTGTTT TGGACGGAGA CAAAGACAGC 
ATTAACTGTG CTAGGCAGGA GGCCATCCGC ATGGTACGCG TTCTGACTGT GCTCAAGGAG 
TATATCAACG AGTGTGACAG CGACTACCAC GAGGAGAGAA CCATACTGCC CATGTCCAGA 
GCTTTCAGGG GAAAGCACAT AACTTTGATT GTGCGTTTCC CAAACCAAGG TCGCCAAGTA 
GATGATTTAG ATATCTGGTC CCACACCAAC GACACGATTG GCTCGGTGAG GCGGGCGATT 
CTAAACAGAA TAAAGGCTAA TGCTGCGCAT ACAAAGGTCG AGTTGTTTAT TGGGGGTGAG 
GTGGTGGATC CAGCTGATGA CAGGAAGTTG ATCGGGCAGC TCAGTCTGAA GGACAAAACG 
TTGATCACGG CCAAGCTGAC CCAGGTGAGT GCCAACATGC CCTCAAGTCC AGACAGCTCC 
TCTGACTCGT CTACTGGCTC TCCTGGTAAC CATGGAAACC ACTACAGCGA CGGGCCCAAC 
CCTGAGGTGG AAAGCTGTCT GCCTGGCGTG ATTATGTCGC TGCATCTGCG GTACATCTCC 
TTCCTGTGGC AGATCGCTGA TCTAGGCTGC AGCCTCAACA TGCCTCTACT TAGAGATGGA 
GCTCGTGTTC TCATGAAGCT TATGCCTCCT GATAACACCA CAGTAGAGAA TCTGAGAGCT 
GTGTGTTTGG ACCATGCCAA GCTGGGAGAG AACAGCCTGA GTCCCTCACT GGACTCCTGC 
TTCTTTGGCC CCTCCCCCTC ACAAGTGCTC TACCTCATAG AGGTCGTGTA TGCTTTGCTG 
ATGCCAGCCA GTGCCACTCT CGGTGAAGAC GCCAGCGACT TCCAATACAA CTTCCTGAAG 
AGCGGCGGCC TGCCCTTGGT GCTGAGCATG CTCACCAGAA ACAACTTTCT CCCATCGGCA 
GACATGGAAA CTCGAAGGGG CGCCTATGTC AACGCACTAA AGATCGCAAA GCTCCTACTG 
ACCGCTGTGG GTTTTGGACA CGTGAAGGCG GTAGCTGAGG CCTGCCAGCC AAACGCCGAC 
GGAAATATTC CCGTCTCTCC AATTAATCAG GCAACGCACG ACCAAGCGCT GGTTCTTCAG 
AGCGCCCTGC AAAGCATCCC TAACCCGGCC TCAGAGTGCA TGCTGCGTAA TGTAGCCATT 
CGCCTGGCCC AACAGATCTC TGACGAGAAC TCTATTTGCC ATCAGAACTT TTTCCAGGCG 
TCGAAGTACA TCCCAGACAT TTGTGTAATC CGAGCAATAC AGAAAATCGT GTGGGCATCA 
GGCTGTGGTA CAGTGCAGCT CGTCTTTAGC TCCAATGAGG AGATCAGCAG GATATATGAG 
AAGACAAATG CTGCCAAGGA ACCAGATGGA GAGGATGAGC AGGTGTGCTG CGAGGCCTTG 
GAGGTCATGA CCCTGTGCTT TGCCCTCATG CCCACGGCTC TGGACACACT AAGCAAAGAG 
AAGGCGTGGC AGACTTTTAT CATAGACCTG TTACTACACT GCCACAGCAA GTCCGTTCGA 
CAAATGGCTC AGGAGCAGTT TTTCCTCATG GCCACAAGAT GCTGCATGGG CCACCGACCC 
CTCCTCTTCT TCATCACCCT GTTATTCACT GTGTTGGGGA GCACGGCCAA AGAGAGAGCC 
AAACATGCGG GGGACTACTT CACCTTGCTC AGACACCTGC TAAACTATGC TTACAACAGC 
AACATCAACC TGCCAAACGC CGAGGTGCTG CTTAACAATG AGATTGACTG GCTCAAGAGA 
ATACGGGATG AGGTGAAGAG AACAGGAGAG ACTGGTGTGG AGGAGACGAT CCTGGAGGGC 
CACCTTGGAG TCACCAAAGA GCTCCTTGCT TTTCAAACAC CGGAAAAGAA GTACTACATT 
GGCAGCGAGA AGGGAGGAGC CAACCTTATA AAGGAGCTGA TCGATGACTT CATATTCCCA 
GCATCAAATG TTTACCTGCA GTACATGAAG ACGGGAGAGT TCCCCACAGA GCAGGCCATC 
CCAGTCTGCA GCACTCCCGC CTCCATCAAT GCTGGCTTTG AGCTCCTGGT GGCTCTGGCC 
GTTGGCTGCA TGCGCAATCT GAAACAAATA GTTGACACCC TGACTGATAT GTACTACTCA 
GGCGGGGAGA CGTTGACAGA GTGGGAGTAC TTGCCTCCAG TTGGGCCACG ACCCAATAAA 
GGCTTTGTAG GTCTAAAGAA CGCCGGTGCC ACATGTTACA TGAACTCTGT CATTCAGCAA 
CTGTACATGA TCCCTCCCAT TCGCAACGGC ATTCTGGCTA TTGAGGGAAC AGGCACGGAT 
GTGGACGATG ACATGTCGGG GGATGAGAAG CAGGAGAATG AGAGTAACGT CGATCCTCGT 
GATGAGGTCT TCAGCTACCA TCACCAGTTT GATGACAAAC CGTCCAGTAA GTCAGAGGAC 
AGGAAGGAGT ACAACATCGG CGTGCTTCGT CACTTGCAGG TCATCTTTGG TCATCTTGCT 
GCATCCAGGC TGCAGTACTA CGTCCCAAGA GGCTTCTGGA AACAGTTTAG ATTGTGGGGT 
GAGCCAGTCA ACCTGAGGGA ACAGCACGAT GCTCTGGAGT TTTTCAACTC TTTGGTGGAC 
AGTTTGGATG AAGCTCTGAA GGCTCTGGGT CACCCCGCCA TGCTCAGTAA GGTGCTGGGA 
GGCTCTTTCG CCGATCAGAA AATCTGTCAA GGGTGCCCTC ACAGGTATGA GTGTGAGGAG 
TCGTTTACAA CACTTAACGT GGACATCAGA AACCACCAAA ACCTGTTGGA CTCCATGGAG 
CAGTATGTCA AAGGAGATCT GCTGGAAGGA GCAAATGCCT ATCACTGTGA GAAGTGTAAT 
AAAAAGGTGG ACACCGTGAA GCGTCTGTTG ATCAAGAAGC TCCCACCCGT CCTGGCCATT 
CAGCTGAAGC GCTTTGACTA CGACTGGGAG CGAGAATGTG CCATCAAGTT TAACGACTAC 
TTTGAGTTCC CCAGGGAGCT GGATATGGAG CCTTACACGG TAGCGGGCGT GGCCAAACTG 
GAGGGCGACG ACGTGAACCC GGAGAACCAG GTCATACAGC AGAACGAGCC GTCTGAATCC 
ACACCGCCGG GCAGCTCCAA ATATCGTCTG GTGGGAGTGC TGGTCCACTC AGGCCAGGCC 
AGTGGCGGAC ACTACTATTC CTACATAATC CAGAGGAACG GAGGGGACGG GGAGAAGAAC 
CGCTGGTATA AGTTCGACGA CGGCGACGTC ACTGAATGCA AGATGGACGA CGAGGAGGAG 
ATGAAGAACC AGTGCTTCGG TGGGGAGTAC ATGGGCGAGG TGTTTGATCA TATGATGAAA 
AGGATGTCGT ATCGGAGGCA AAAGCGCTGG TGGAACGCTT ACATCCTGTT TTACGAGCGA 
ATGGACTCGT TGGACAAGGA CAGCGATCTC GTCAAATACA TCTCGGACCT GACGATCTCC 
ACCAAGCCAC ATCAGGTCAA GATGCCCACG GTCATCGAGT GCAGCGTCCG CAAGCAGAAC 
GTCCAGTTCA TGCACAACCG AATGCAATAC AGCCTAGAAT ATTTCCAGTT CATAAAGAAA 
CTTCTGACCT GTAACAGTGT CTATTTAAAC CCTCCTCCAG GGCAAGACCA CCTGCTGCCA 
GAGGCAGAGG AGATCGCGAT GATCAGCACT CAGCTGGCTG CTAGGTTCCT CTTCAGCACA 
GGTTTCCACA CCAAGAAAGT AGTACGGGGT CCTGCCAGTG ACTGGTATGA CGCCCTCTGC 
ATCCTGCTGA GACACAGTAA GAATGTTCGT TACTGGTTTG CACACAACGT CCTGTTTGCG 
TACCCTAACC GCTTCTCCGA GTATTTGCTG GAGTGCCCCA GCGCTGAGGT CCGCGGAGCA 
TTTGCCAAGC TCATCGTCTT CATCGCTCAC TTCTCCCTGC AAGATGGCCC CTGCCCCTCC 
CCCACCGCCT CACCTGGACC GTCTGCTCAG GGCTGTGATA ATCTCAGTTT AAGTGACCAC 
TTGTTGAGAG CTGTTCTAAA CCTGCTTAGG AGAGAGGTTT CTGAACATGG CCGACATCTA 
CAGCAGTACT TCAACCTCTT TGTCATGTAT GCCAATCTGG GCCTGGCAGA GAAGTCTCAG 
CTGCTGAAGC TCAACGTTCC CGCCACTTTC ATGCTGGTCG CTCTAGACGA GGGTCCCGGT 
CCCCCCATCA AATACCAGTA TGCAGAACTG GGCAAGCTCT ACACTGTGGT TTCCCAGCTG 
GTCCGCTGCT GTGAGGTGTC CTCGCGCATG CAATCCTCCA TCAATGGTAA CCCTCCTCTT 
CCTAACCCGT ATGGGGATCC CAACCTGACG GCTCCGGTGA TGTCTGTGCA GCAGCTGGTG 
GCAGAAATCC TGTTTGTGAG GACGAGCTAC GTCAAGAAGA TCATCGAGGA CTGCAGCAAC 
TCTGAGGAGA CCGTGAAGCT CCTTAGATTC TGCTGCTGGG AGAATCCTCA GTTCTCCTCC 
ACTGTGCTCA GTGAACTGCT TTGGCAGGTG GCATATTCTT ACACGTATGA GCTGAGGCCT 
TACCTGGACT TGCTGCTACA GATCCTTCTC ATCGAAGACT CCTGGCAGAC GCACAGGATC 
CACAATGTCC TAAAGGGCAT CCCTGATGAC AGAGACGGGC TGTTTGACAC CATCCAGCGC 
TCCAAAAACC ATTACCAGAA GAGGGCCTAC CAGTGCATCA AGTGCATGGT GGCCCTCTTT 
AGCAACTGCT CCGTGGCTTA CCAGATCCTG CAGAGTAATG GTGACCTGAA AAGGAAGTGG 
ACGTGGGCGG TGGAGTGGTT GGGGGATGAG TTGGAGAGAA GGCCGTACAC AGGAAACCCG 
CAGTACACCT ACAACAACTG GTCCCCCCCA GTTCAGAGCA ACGAGACCTC CAACGGGTAT 
TTCCTGGAGC GTTCACACAG TGCACGTATG ACACTTGCCA AAGCCTGTGA ACTCTGTCCT 
GAAGAGCTCA AGTGTACACA GGAAAGCCCA GGGAAGGAGC CGGACGAACA GGAAGCACCT 
GATGATCAAG ACTCCTCCCC TCCTGAAGAC ACCTCTCTGT ACCCACATTC TCCTGGAAGC 
ACCCAGTTTC AGCAGAATAA CCACCCCCAT GGGCAGCCTT ACACCGGGCC TGCTGCCCAG 
CACATGAACA ACCCCCAGCG CCCCGGCCCA GCCTCCGCTC CGACTCCCGG CCCGGCCCAG 
ACCCCCGCCT CAGGCCCCGG CACCGCCCCT GGTTCCGGCC CGCGAGCACA AGAGCACTGG 
GAGGGCACAG AGGAAGTCGC CCCCGCCCCC GCCTCCACCC CAGCACCTGT CCCCCCGAAG 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806298.1
CDS	657..8462
Translation	MTATTRGSPVGGNDSQGQGQAPDAQSQPPLPQNQTSSPNSSNENSPVSPPDEQSQVDRPPQLEEEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKCEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMATNPHCKFHIYNGTRPSETVPAGAQLPDDELYARPPDPRSPKGWLVDLINKFGNLNGFQILHDRFMSGQALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPVIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHNPEEEEWLTAERMAEWIQQNHILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTMQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDQALNAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLSAYMETMRQLSKEQAEFDPQTVRPGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVFLCDREACFKWYSKLMGDEPDLDPDINKDFFENNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQGSDDIASRAIDLLKEIYTNLGPKLQVNQVEIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQEAIRMVRVLTVLKEYINECDSDYHEERTILPMSRAFRGKHITLIVRFPNQGRQVDDLDIWSHTNDTIGSVRRAILNRIKANAAHTKVELFIGGEVVDPADDRKLIGQLSLKDKTLITAKLTQVSANMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHLRYISFLWQIADLGCSLNMPLLRDGARVLMKLMPPDNTTVENLRAVCLDHAKLGENSLSPSLDSCFFGPSPSQVLYLIEVVYALLMPASATLGEDASDFQYNFLKSGGLPLVLSMLTRNNFLPSADMETRRGAYVNALKIAKLLLTAVGFGHVKAVAEACQPNADGNIPVSPINQATHDQALVLQSALQSIPNPASECMLRNVAIRLAQQISDENSICHQNFFQASKYIPDICVIRAIQKIVWASGCGTVQLVFSSNEEISRIYEKTNAAKEPDGEDEQVCCEALEVMTLCFALMPTALDTLSKEKAWQTFIIDLLLHCHSKSVRQMAQEQFFLMATRCCMGHRPLLFFITLLFTVLGSTAKERAKHAGDYFTLLRHLLNYAYNSNINLPNAEVLLNNEIDWLKRIRDEVKRTGETGVEETILEGHLGVTKELLAFQTPEKKYYIGSEKGGANLIKELIDDFIFPASNVYLQYMKTGEFPTEQAIPVCSTPASINAGFELLVALAVGCMRNLKQIVDTLTDMYYSGGETLTEWEYLPPVGPRPNKGFVGLKNAGATCYMNSVIQQLYMIPPIRNGILAIEGTGTDVDDDMSGDEKQENESNVDPRDEVFSYHHQFDDKPSSKSEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSMEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDDVNPENQVIQQNEPSESTPPGSSKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDEEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDSLDKDSDLVKYISDLTISTKPHQVKMPTVIECSVRKQNVQFMHNRMQYSLEYFQFIKKLLTCNSVYLNPPPGQDHLLPEAEEIAMISTQLAARFLFSTGFHTKKVVRGPASDWYDALCILLRHSKNVRYWFAHNVLFAYPNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPTASPGPSAQGCDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGLAEKSQLLKLNVPATFMLVALDEGPGPPIKYQYAELGKLYTVVSQLVRCCEVSSRMQSSINGNPPLPNPYGDPNLTAPVMSVQQLVAEILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNVLKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCSVAYQILQSNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEELKCTQESPGKEPDEQEAPDDQDSSPPEDTSLYPHSPGSTQFQQNNHPHGQPYTGPAAQHMNNPQRPGPASAPTPGPAQTPASGPGTAPGSGPRAQEHWEGTEEVAPAPASTPAPVPPKE

Target ORF information:

RefSeq Version	XM_023950530.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950530.1

ORF Insert Sequence:

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGCCAA ACCCAGAGAA GTCCACACGT GTTTTACCGG 
CACGACCTGA TCAACCAGCT GCAGCATAAC CACGCTCTGG TCACCCTCGT GGCCGAGAAC 
CTCTCTGCTT ACATGGAGAC TATGAGGCAG CTATCCAAAG AACAAGCAGA GTTTGATCCT 
CAGACAGTCA GACCAGGAAG CCGCTACAGT CACGTCCAAG AAGTTCAGGA GCGACTCAAC 
TTCCTGAGGT TTCTTTTAAA AGACGGCCAG CTGTGGCTGT GTGCCCCTCA AGCCAAGCAG 
ATCTGGAAGT GTCTGGCTGA GAATGCTGTG TTTCTCTGCG ATCGCGAAGC TTGCTTCAAA 
TGGTACTCCA AGCTGATGGG GGATGAACCA GACCTGGATC CGGACATCAA TAAGGACTTC 
TTTGAGAATA ACGTTCTGCA GTTGGATCCG TCTCTGCTGA CGGAAAATGG CATGAAGTGC 
TTTGAGAGGT TCTTCAAGGC CGTCAACTGC AGGGAGGGGA AGCTGGTTGC AAAGCGCAGG 
GCTTACATGA TGGATGACTT GGAGCTAATA GGCCTGGACT ATCTGTGGAG GGTGGTAATT 
CAAGGAAGCG ATGACATTGC TAGCCGAGCC ATAGACCTGC TGAAAGAGAT TTACACCAAC 
CTTGGTCCCA AACTACAAGT CAATCAGGTT GAGATTCACG AGGATTTCAT CCAGTCCTGC 
TTCGACCGTC TGAAGGCGTC TTATGATACC CTTTGTGTTT TGGACGGAGA CAAAGACAGC 
ATTAACTGTG CTAGGCAGGA GGCCATCCGC ATGGTACGCG TTCTGACTGT GCTCAAGGAG 
TATATCAACG AGTGTGACAG CGACTACCAC GAGGAGAGAA CCATACTGCC CATGTCCAGA 
GCTTTCAGGG GAAAGCACAT AACTTTGATT GTGCGTTTCC CAAACCAAGG TCGCCAAGTA 
GATGATTTAG ATATCTGGTC CCACACCAAC GACACGATTG GCTCGGTGAG GCGGGCGATT 
CTAAACAGAA TAAAGGCTAA TGCTGCGCAT ACAAAGGTCG AGTTGTTTAT TGGGGGTGAG 
GTGGTGGATC CAGCTGATGA CAGGAAGTTG ATCGGGCAGC TCAGTCTGAA GGACAAAACG 
TTGATCACGG CCAAGCTGAC CCAGGTGAGT GCCAACATGC CCTCAAGTCC AGACAGCTCC 
TCTGACTCGT CTACTGGCTC TCCTGGTAAC CATGGAAACC ACTACAGCGA CGGGCCCAAC 
CCTGAGGTGG AAAGCTGTCT GCCTGGCGTG ATTATGTCGC TGCATCTGCG GTACATCTCC 
TTCCTGTGGC AGATCGCTGA TCTAGGCTGC AGCCTCAACA TGCCTCTACT TAGAGATGGA 
GCTCGTGTTC TCATGAAGCT TATGCCTCCT GATAACACCA CAGTAGAGAA TCTGAGAGCT 
GTGTGTTTGG ACCATGCCAA GCTGGGAGAG AACAGCCTGA GTCCCTCACT GGACTCCTGC 
TTCTTTGGCC CCTCCCCCTC ACAAGTGCTC TACCTCATAG AGGTCGTGTA TGCTTTGCTG 
ATGCCAGCCA GTGCCACTCT CGGTGAAGAC GCCAGCGACT TCCAATACAA CTTCCTGAAG 
AGCGGCGGCC TGCCCTTGGT GCTGAGCATG CTCACCAGAA ACAACTTTCT CCCATCGGCA 
GACATGGAAA CTCGAAGGGG CGCCTATGTC AACGCACTAA AGATCGCAAA GCTCCTACTG 
ACCGCTGTGG GTTTTGGACA CGTGAAGGCG GTAGCTGAGG CCTGCCAGCC AAACGCCGAC 
GGAAATATTC CCGTCTCTCC AATTAATCAG GCAACGCACG ACCAAGCGCT GGTTCTTCAG 
AGCGCCCTGC AAAGCATCCC TAACCCGGCC TCAGAGTGCA TGCTGCGTAA TGTAGCCATT 
CGCCTGGCCC AACAGATCTC TGACGAGAAC TCTATTTGCC ATCAGAACTT TTTCCAGGCG 
TCGAAGTACA TCCCAGACAT TTGTGTAATC CGAGCAATAC AGAAAATCGT GTGGGCATCA 
GGCTGTGGTA CAGTGCAGCT CGTCTTTAGC TCCAATGAGG AGATCAGCAG GATATATGAG 
AAGACAAATG CTGCCAAGGA ACCAGATGGA GAGGATGAGC AGGTGTGCTG CGAGGCCTTG 
GAGGTCATGA CCCTGTGCTT TGCCCTCATG CCCACGGCTC TGGACACACT AAGCAAAGAG 
AAGGCGTGGC AGACTTTTAT CATAGACCTG TTACTACACT GCCACAGCAA GTCCGTTCGA 
CAAATGGCTC AGGAGCAGTT TTTCCTCATG GCCACAAGAT GCTGCATGGG CCACCGACCC 
CTCCTCTTCT TCATCACCCT GTTATTCACT GTGTTGGGGA GCACGGCCAA AGAGAGAGCC 
AAACATGCGG GGGACTACTT CACCTTGCTC AGACACCTGC TAAACTATGC TTACAACAGC 
AACATCAACC TGCCAAACGC CGAGGTGCTG CTTAACAATG AGATTGACTG GCTCAAGAGA 
ATACGGGATG AGGTGAAGAG AACAGGAGAG ACTGGTGTGG AGGAGACGAT CCTGGAGGGC 
CACCTTGGAG TCACCAAAGA GCTCCTTGCT TTTCAAACAC CGGAAAAGAA GTACTACATT 
GGCAGCGAGA AGGGAGGAGC CAACCTTATA AAGGAGCTGA TCGATGACTT CATATTCCCA 
GCATCAAATG TTTACCTGCA GTACATGAAG ACGGGAGAGT TCCCCACAGA GCAGGCCATC 
CCAGTCTGCA GCACTCCCGC CTCCATCAAT GCTGGCTTTG AGCTCCTGGT GGCTCTGGCC 
GTTGGCTGCA TGCGCAATCT GAAACAAATA GTTGACACCC TGACTGATAT GTACTACTCA 
GGCGGGGAGA CGTTGACAGA GTGGGAGTAC TTGCCTCCAG TTGGGCCACG ACCCAATAAA 
GGCTTTGTAG GTCTAAAGAA CGCCGGTGCC ACATGTTACA TGAACTCTGT CATTCAGCAA 
CTGTACATGA TCCCTCCCAT TCGCAACGGC ATTCTGGCTA TTGAGGGAAC AGGCACGGAT 
GTGGACGATG ACATGTCGGG GGATGAGAAG CAGGAGAATG AGAGTAACGT CGATCCTCGT 
GATGAGGTCT TCAGCTACCA TCACCAGTTT GATGACAAAC CGTCCAGTAA GTCAGAGGAC 
AGGAAGGAGT ACAACATCGG CGTGCTTCGT CACTTGCAGG TCATCTTTGG TCATCTTGCT 
GCATCCAGGC TGCAGTACTA CGTCCCAAGA GGCTTCTGGA AACAGTTTAG ATTGTGGGGT 
GAGCCAGTCA ACCTGAGGGA ACAGCACGAT GCTCTGGAGT TTTTCAACTC TTTGGTGGAC 
AGTTTGGATG AAGCTCTGAA GGCTCTGGGT CACCCCGCCA TGCTCAGTAA GGTGCTGGGA 
GGCTCTTTCG CCGATCAGAA AATCTGTCAA GGGTGCCCTC ACAGGTATGA GTGTGAGGAG 
TCGTTTACAA CACTTAACGT GGACATCAGA AACCACCAAA ACCTGTTGGA CTCCATGGAG 
CAGTATGTCA AAGGAGATCT GCTGGAAGGA GCAAATGCCT ATCACTGTGA GAAGTGTAAT 
AAAAAGGTGG ACACCGTGAA GCGTCTGTTG ATCAAGAAGC TCCCACCCGT CCTGGCCATT 
CAGCTGAAGC GCTTTGACTA CGACTGGGAG CGAGAATGTG CCATCAAGTT TAACGACTAC 
TTTGAGTTCC CCAGGGAGCT GGATATGGAG CCTTACACGG TAGCGGGCGT GGCCAAACTG 
GAGGGCGACG ACGTGAACCC GGAGAACCAG GTCATACAGC AGAACGAGCC GTCTGAATCC 
ACACCGCCGG GCAGCTCCAA ATATCGTCTG GTGGGAGTGC TGGTCCACTC AGGCCAGGCC 
AGTGGCGGAC ACTACTATTC CTACATAATC CAGAGGAACG GAGGGGACGG GGAGAAGAAC 
CGCTGGTATA AGTTCGACGA CGGCGACGTC ACTGAATGCA AGATGGACGA CGAGGAGGAG 
ATGAAGAACC AGTGCTTCGG TGGGGAGTAC ATGGGCGAGG TGTTTGATCA TATGATGAAA 
AGGATGTCGT ATCGGAGGCA AAAGCGCTGG TGGAACGCTT ACATCCTGTT TTACGAGCGA 
ATGGACTCGT TGGACAAGGA CAGCGATCTC GTCAAATACA TCTCGGACCT GACGATCTCC 
ACCAAGCCAC ATCAGGTCAA GATGCCCACG GTCATCGAGT GCAGCGTCCG CAAGCAGAAC 
GTCCAGTTCA TGCACAACCG AATGCAATAC AGCCTAGAAT ATTTCCAGTT CATAAAGAAA 
CTTCTGACCT GTAACAGTGT CTATTTAAAC CCTCCTCCAG GGCAAGACCA CCTGCTGCCA 
GAGGCAGAGG AGATCGCGAT GATCAGCACT CAGCTGGCTG CTAGGTTCCT CTTCAGCACA 
GGTTTCCACA CCAAGAAAGT AGTACGGGGT CCTGCCAGTG ACTGGTATGA CGCCCTCTGC 
ATCCTGCTGA GACACAGTAA GAATGTTCGT TACTGGTTTG CACACAACGT CCTGTTTGCG 
TACCCTAACC GCTTCTCCGA GTATTTGCTG GAGTGCCCCA GCGCTGAGGT CCGCGGAGCA 
TTTGCCAAGC TCATCGTCTT CATCGCTCAC TTCTCCCTGC AAGATGGCCC CTGCCCCTCC 
CCCACCGCCT CACCTGGACC GTCTGCTCAG GGCTGTGATA ATCTCAGTTT AAGTGACCAC 
TTGTTGAGAG CTGTTCTAAA CCTGCTTAGG AGAGAGGTTT CTGAACATGG CCGACATCTA 
CAGCAGTACT TCAACCTCTT TGTCATGTAT GCCAATCTGG GCCTGGCAGA GAAGTCTCAG 
CTGCTGAAGC TCAACGTTCC CGCCACTTTC ATGCTGGTCG CTCTAGACGA GGGTCCCGGT 
CCCCCCATCA AATACCAGTA TGCAGAACTG GGCAAGCTCT ACACTGTGGT TTCCCAGCTG 
GTCCGCTGCT GTGAGGTGTC CTCGCGCATG CAATCCTCCA TCAATGGTAA CCCTCCTCTT 
CCTAACCCGT ATGGGGATCC CAACCTGACG GCTCCGGTGA TGTCTGTGCA GCAGCTGGTG 
GCAGAAATCC TGTTTGTGAG GACGAGCTAC GTCAAGAAGA TCATCGAGGA CTGCAGCAAC 
TCTGAGGAGA CCGTGAAGCT CCTTAGATTC TGCTGCTGGG AGAATCCTCA GTTCTCCTCC 
ACTGTGCTCA GTGAACTGCT TTGGCAGGTG GCATATTCTT ACACGTATGA GCTGAGGCCT 
TACCTGGACT TGCTGCTACA GATCCTTCTC ATCGAAGACT CCTGGCAGAC GCACAGGATC 
CACAATGTCC TAAAGGGCAT CCCTGATGAC AGAGACGGGC TGTTTGACAC CATCCAGCGC 
TCCAAAAACC ATTACCAGAA GAGGGCCTAC CAGTGCATCA AGTGCATGGT GGCCCTCTTT 
AGCAACTGCT CCGTGGCTTA CCAGATCCTG CAGAGTAATG GTGACCTGAA AAGGAAGTGG 
ACGTGGGCGG TGGAGTGGTT GGGGGATGAG TTGGAGAGAA GGCCGTACAC AGGAAACCCG 
CAGTACACCT ACAACAACTG GTCCCCCCCA GTTCAGAGCA ACGAGACCTC CAACGGGTAT 
TTCCTGGAGC GTTCACACAG TGCACGTATG ACACTTGCCA AAGCCTGTGA ACTCTGTCCT 
GAAGAGCTCA AGTGTACACA GGAAAGCCCA GGGAAGGAGC CGGACGAACA GGAAGCACCT 
GATGATCAAG ACTCCTCCCC TCCTGAAGAC ACCTCTCTGT ACCCACATTC TCCTGGAAGC 
ACCCAGTTTC AGCAGAATAA CCACCCCCAT GGGCAGCCTT ACACCGGGCC TGCTGCCCAG 
CACATGAACA ACCCCCAGCG CCCCGGCCCA GCCTCCGCTC CGACTCCCGG CCCGGCCCAG 
ACCCCCGCCT CAGGCCCCGG CACCGCCCCT GGTTCCGGCC CGCGAGCACA AGAGCACTGG 
GAGGGCACAG AGGAAGTCGC CCCCGCCCCC GCCTCCACCC CAGCACCTGT CCCCCCGAAG 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp9x ( XM_023950531.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023806299.1 Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X7, mRNA.

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CloneID	OJa113032
Clone ID Related Accession (Same CDS sequence)	XM_023950531.1
Accession Version	XM_023950531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGCCAA ACCCAGAGAA GTCCACACGT GTTTTACCGG 
CACGACCTGA TCAACCAGCT GCAGCATAAC CACGCTCTGG TCACCCTCGT GGCCGAGAAC 
CTCTCTGCTT ACATGGAGAC TATGAGGCAG CTATCCAAAG AACAAGCAGA GTTTGATCCT 
CAGACAGTCA GACCAGGAAG CCGCTACAGT CACGTCCAAG AAGTTCAGGA GCGACTCAAC 
TTCCTGAGGT TTCTTTTAAA AGACGGCCAG CTGTGGCTGT GTGCCCCTCA AGCCAAGCAG 
ATCTGGAAGT GTCTGGCTGA GAATGCTGTG TTTCTCTGCG ATCGCGAAGC TTGCTTCAAA 
TGGTACTCCA AGCTGATGGG GGATGAACCA GACCTGGATC CGGACATCAA TAAGGACTTC 
TTTGAGAATA ACGTTCTGCA GTTGGATCCG TCTCTGCTGA CGGAAAATGG CATGAAGTGC 
TTTGAGAGGT TCTTCAAGGC CGTCAACTGC AGGGAGGGGA AGCTGGTTGC AAAGCGCAGG 
GCTTACATGA TGGATGACTT GGAGCTAATA GGCCTGGACT ATCTGTGGAG GGTGGTAATT 
CAAGGAAGCG ATGACATTGC TAGCCGAGCC ATAGACCTGC TGAAAGAGAT TTACACCAAC 
CTTGGTCCCA AACTACAAGT CAATCAGGTT GAGATTCACG AGGATTTCAT CCAGTCCTGC 
TTCGACCGTC TGAAGGCGTC TTATGATACC CTTTGTGTTT TGGACGGAGA CAAAGACAGC 
ATTAACTGTG CTAGGCAGGA GGCCATCCGC ATGGTACGCG TTCTGACTGT GCTCAAGGAG 
TATATCAACG AGTGTGACAG CGACTACCAC GAGGAGAGAA CCATACTGCC CATGTCCAGA 
GCTTTCAGGG GAAAGCACAT AACTTTGATT GTGCGTTTCC CAAACCAAGG TCGCCAAGTA 
GATGATTTAG ATATCTGGTC CCACACCAAC GACACGATTG GCTCGGTGAG GCGGGCGATT 
CTAAACAGAA TAAAGGCTAA TGCTGCGCAT ACAAAGGTCG AGTTGTTTAT TGGGGGTGAG 
GTGGTGGATC CAGCTGATGA CAGGAAGTTG ATCGGGCAGC TCAGTCTGAA GGACAAAACG 
TTGATCACGG CCAAGCTGAC CCAGGTGAGT GCCAACATGC CCTCAAGTCC AGACAGCTCC 
TCTGACTCGT CTACTGGCTC TCCTGGTAAC CATGGAAACC ACTACAGCGA CGGGCCCAAC 
CCTGAGGTGG AAAGCTGTCT GCCTGGCGTG ATTATGTCGC TGCATCTGCG GTACATCTCC 
TTCCTGTGGC AGATCGCTGA TCTAGGCTGC AGCCTCAACA TGCCTCTACT TAGAGATGGA 
GCTCGTGTTC TCATGAAGCT TATGCCTCCT GATAACACCA CAGTAGAGAA TCTGAGAGCT 
GTGTGTTTGG ACCATGCCAA GCTGGGAGAG AACAGCCTGA GTCCCTCACT GGACTCCTGC 
TTCTTTGGCC CCTCCCCCTC ACAAGTGCTC TACCTCATAG AGGTCGTGTA TGCTTTGCTG 
ATGCCAGCCA GTGCCACTCT CGGTGAAGAC GCCAGCGACT TCCAATACAA CTTCCTGAAG 
AGCGGCGGCC TGCCCTTGGT GCTGAGCATG CTCACCAGAA ACAACTTTCT CCCATCGGCA 
GACATGGAAA CTCGAAGGGG CGCCTATGTC AACGCACTAA AGATCGCAAA GCTCCTACTG 
ACCGCTGTGG GTTTTGGACA CGTGAAGGCG GTAGCTGAGG CCTGCCAGCC AAACGCCGAC 
GGAAATATTC CCGTCTCTCC AATTAATCAG GCAACGCACG ACCAAGCGCT GGTTCTTCAG 
AGCGCCCTGC AAAGCATCCC TAACCCGGCC TCAGAGTGCA TGCTGCGTAA TGTAGCCATT 
CGCCTGGCCC AACAGATCTC TGACGAGAAC TCTATTTGCC ATCAGAACTT TTTCCAGGCG 
TCGAAGTACA TCCCAGACAT TTGTGTAATC CGAGCAATAC AGAAAATCGT GTGGGCATCA 
GGCTGTGGTA CAGTGCAGCT CGTCTTTAGC TCCAATGAGG AGATCAGCAG GATATATGAG 
AAGACAAATG CTGCCAAGGA ACCAGATGGA GAGGATGAGC AGGTGTGCTG CGAGGCCTTG 
GAGGTCATGA CCCTGTGCTT TGCCCTCATG CCCACGGCTC TGGACACACT AAGCAAAGAG 
AAGGCGTGGC AGACTTTTAT CATAGACCTG TTACTACACT GCCACAGCAA GTCCGTTCGA 
CAAATGGCTC AGGAGCAGTT TTTCCTCATG GCCACAAGAT GCTGCATGGG CCACCGACCC 
CTCCTCTTCT TCATCACCCT GTTATTCACT GTGTTGGGGA GCACGGCCAA AGAGAGAGCC 
AAACATGCGG GGGACTACTT CACCTTGCTC AGACACCTGC TAAACTATGC TTACAACAGC 
AACATCAACC TGCCAAACGC CGAGGTGCTG CTTAACAATG AGATTGACTG GCTCAAGAGA 
ATACGGGATG AGGTGAAGAG AACAGGAGAG ACTGGTGTGG AGGAGACGAT CCTGGAGGGC 
CACCTTGGAG TCACCAAAGA GCTCCTTGCT TTTCAAACAC CGGAAAAGAA GTACTACATT 
GGCAGCGAGA AGGGAGGAGC CAACCTTATA AAGGAGCTGA TCGATGACTT CATATTCCCA 
GCATCAAATG TTTACCTGCA GTACATGAAG ACGGGAGAGT TCCCCACAGA GCAGGCCATC 
CCAGTCTGCA GCACTCCCGC CTCCATCAAT GCTGGCTTTG AGCTCCTGGT GGCTCTGGCC 
GTTGGCTGCA TGCGCAATCT GAAACAAATA GTTGACACCC TGACTGATAT GTACTACTCA 
GGCGGGGAGA CGTTGACAGA GTGGGAGTAC TTGCCTCCAG TTGGGCCACG ACCCAATAAA 
GGCTTTGTAG GTCTAAAGAA CGCCGGTGCC ACATGTTACA TGAACTCTGT CATTCAGCAA 
CTGTACATGA TCCCTCCCAT TCGCAACGGC ATTCTGGCTA TTGAGGGAAC AGGCACGGAT 
GTGGACGATG ACATGTCGGG GGATGAGAAG CAGGAGAATG AGAGTAACGT CGATCCTCGT 
GATGAGGTCT TCAGCTACCA TCACCAGTTT GATGACAAAC CGTCCAGTAA GTCAGAGGAC 
AGGAAGGAGT ACAACATCGG CGTGCTTCGT CACTTGCAGG TCATCTTTGG TCATCTTGCT 
GCATCCAGGC TGCAGTACTA CGTCCCAAGA GGCTTCTGGA AACAGTTTAG ATTGTGGGGT 
GAGCCAGTCA ACCTGAGGGA ACAGCACGAT GCTCTGGAGT TTTTCAACTC TTTGGTGGAC 
AGTTTGGATG AAGCTCTGAA GGCTCTGGGT CACCCCGCCA TGCTCAGTAA GGTGCTGGGA 
GGCTCTTTCG CCGATCAGAA AATCTGTCAA GGGTGCCCTC ACAGGTATGA GTGTGAGGAG 
TCGTTTACAA CACTTAACGT GGACATCAGA AACCACCAAA ACCTGTTGGA CTCCATGGAG 
CAGTATGTCA AAGGAGATCT GCTGGAAGGA GCAAATGCCT ATCACTGTGA GAAGTGTAAT 
AAAAAGGTGG ACACCGTGAA GCGTCTGTTG ATCAAGAAGC TCCCACCCGT CCTGGCCATT 
CAGCTGAAGC GCTTTGACTA CGACTGGGAG CGAGAATGTG CCATCAAGTT TAACGACTAC 
TTTGAGTTCC CCAGGGAGCT GGATATGGAG CCTTACACGG TAGCGGGCGT GGCCAAACTG 
GAGGGCGACG ACGTGAACCC GGAGAACCAG GTCATACAGC AGAACGAGCC GTCTGAATCC 
ACACCGCCGG GCAGCTCCAA ATATCGTCTG GTGGGAGTGC TGGTCCACTC AGGCCAGGCC 
AGTGGCGGAC ACTACTATTC CTACATAATC CAGAGGAACG GAGGGGACGG GGAGAAGAAC 
CGCTGGTATA AGTTCGACGA CGGCGACGTC ACTGAATGCA AGATGGACGA CGAGGAGGAG 
ATGAAGAACC AGTGCTTCGG TGGGGAGTAC ATGGGCGAGG TGTTTGATCA TATGATGAAA 
AGGATGTCGT ATCGGAGGCA AAAGCGCTGG TGGAACGCTT ACATCCTGTT TTACGAGCGA 
ATGGACTCGT TGGACAAGGA CAGCGATCTC GTCAAATACA TCTCGGACCT GACGATCTCC 
ACCAAGCCAC ATCAGGTCAA GATGCCCACG GTCATCGAGT GCAGCGTCCG CAAGCAGAAC 
GTCCAGTTCA TGCACAACCG AATGCAATAC AGCCTAGAAT ATTTCCAGTT CATAAAGAAA 
CTTCTGACCT GTAACAGTGT CTATTTAAAC CCTCCTCCAG GGCAAGACCA CCTGCTGCCA 
GAGGCAGAGG AGATCGCGAT GATCAGCACT CAGCTGGCTG CTAGGTTCCT CTTCAGCACA 
GGTTTCCACA CCAAGAAAGT AGTACGGGGT CCTGCCAGTG ACTGGTATGA CGCCCTCTGC 
ATCCTGCTGA GACACAGTAA GAATGTTCGT TACTGGTTTG CACACAACGT CCTGTTTGCG 
TACCCTAACC GCTTCTCCGA GTATTTGCTG GAGTGCCCCA GCGCTGAGGT CCGCGGAGCA 
TTTGCCAAGC TCATCGTCTT CATCGCTCAC TTCTCCCTGC AAGATGGCCC CTGCCCCTCC 
CCCACCGCCT CACCTGGACC GTCTGCTCAG GGCTGTGATA ATCTCAGTTT AAGTGACCAC 
TTGTTGAGAG CTGTTCTAAA CCTGCTTAGG AGAGAGGTTT CTGAACATGG CCGACATCTA 
CAGCAGTACT TCAACCTCTT TGTCATGTAT GCCAATCTGG GCCTGGCAGA GAAGTCTCAG 
CTGCTGAAGC TCAACGTTCC CGCCACTTTC ATGCTGGTCG CTCTAGACGA GGGTCCCGGT 
CCCCCCATCA AATACCAGTA TGCAGAACTG GGCAAGCTCT ACACTGTGGT TTCCCAGCTG 
GTCCGCTGCT GTGAGGTGTC CTCGCGCATG CAATCCTCCA TCAATGGTAA CCCTCCTCTT 
CCTAACCCGT ATGGGGATCC CAACCTGACG GCTCCGGTGA TGTCTGTGCA GCAGCTGGTG 
GCAGAAATCC TGTTTGTGAG GACGAGCTAC GTCAAGAAGA TCATCGAGGA CTGCAGCAAC 
TCTGAGGAGA CCGTGAAGCT CCTTAGATTC TGCTGCTGGG AGAATCCTCA GTTCTCCTCC 
ACTGTGCTCA GTGAACTGCT TTGGCAGGTG GCATATTCTT ACACGTATGA GCTGAGGCCT 
TACCTGGACT TGCTGCTACA GATCCTTCTC ATCGAAGACT CCTGGCAGAC GCACAGGATC 
CACAATGTCC TAAAGGGCAT CCCTGATGAC AGAGACGGGC TGTTTGACAC CATCCAGCGC 
TCCAAAAACC ATTACCAGAA GAGGGCCTAC CAGTGCATCA AGTGCATGGT GGCCCTCTTT 
AGCAACTGCT CCGTGGCTTA CCAGATCCTG CAGAGTAATG GTGACCTGAA AAGGAAGTGG 
ACGTGGGCGG TGGAGTGGTT GGGGGATGAG TTGGAGAGAA GGCCGTACAC AGGAAACCCG 
CAGTACACCT ACAACAACTG GTCCCCCCCA GTTCAGAGCA ACGAGACCTC CAACGGGTAT 
TTCCTGGAGC GTTCACACAG TGCACGTATG ACACTTGCCA AAGCCTGTGA ACTCTGTCCT 
GAAGAGGAGC CGGACGAACA GGAAGCACCT GATGATCAAG ACTCCTCCCC TCCTGAAGAC 
ACCTCTCTGT ACCCACATTC TCCTGGAAGC ACCCAGTTTC AGCAGAATAA CCACCCCCAT 
GGGCAGCCTT ACACCGGGCC TGCTGCCCAG CACATGAACA ACCCCCAGCG CCCCGGCCCA 
GCCTCCGCTC CGACTCCCGG CCCGGCCCAG ACCCCCGCCT CAGGCCCCGG CACCGCCCCT 
GGTTCCGGCC CGCGAGCACA AGAGCACTGG GAGGGCACAG AGGAAGTCGC CCCCGCCCCC 
GCCTCCACCC CAGCACCTGT CCCCCCGAAG GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023806299.1
CDS	658..8433
Translation	MTATTRGSPVGGNDSQGQGQAPDAQSQPPLPQNQTSSPNSSNENSPVSPPDEQSQVDRPPQLEEEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKCEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMATNPHCKFHIYNGTRPSETVPAGAQLPDDELYARPPDPRSPKGWLVDLINKFGNLNGFQILHDRFMSGQALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPVIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHNPEEEEWLTAERMAEWIQQNHILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTMQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDQALNAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLSAYMETMRQLSKEQAEFDPQTVRPGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVFLCDREACFKWYSKLMGDEPDLDPDINKDFFENNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQGSDDIASRAIDLLKEIYTNLGPKLQVNQVEIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQEAIRMVRVLTVLKEYINECDSDYHEERTILPMSRAFRGKHITLIVRFPNQGRQVDDLDIWSHTNDTIGSVRRAILNRIKANAAHTKVELFIGGEVVDPADDRKLIGQLSLKDKTLITAKLTQVSANMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHLRYISFLWQIADLGCSLNMPLLRDGARVLMKLMPPDNTTVENLRAVCLDHAKLGENSLSPSLDSCFFGPSPSQVLYLIEVVYALLMPASATLGEDASDFQYNFLKSGGLPLVLSMLTRNNFLPSADMETRRGAYVNALKIAKLLLTAVGFGHVKAVAEACQPNADGNIPVSPINQATHDQALVLQSALQSIPNPASECMLRNVAIRLAQQISDENSICHQNFFQASKYIPDICVIRAIQKIVWASGCGTVQLVFSSNEEISRIYEKTNAAKEPDGEDEQVCCEALEVMTLCFALMPTALDTLSKEKAWQTFIIDLLLHCHSKSVRQMAQEQFFLMATRCCMGHRPLLFFITLLFTVLGSTAKERAKHAGDYFTLLRHLLNYAYNSNINLPNAEVLLNNEIDWLKRIRDEVKRTGETGVEETILEGHLGVTKELLAFQTPEKKYYIGSEKGGANLIKELIDDFIFPASNVYLQYMKTGEFPTEQAIPVCSTPASINAGFELLVALAVGCMRNLKQIVDTLTDMYYSGGETLTEWEYLPPVGPRPNKGFVGLKNAGATCYMNSVIQQLYMIPPIRNGILAIEGTGTDVDDDMSGDEKQENESNVDPRDEVFSYHHQFDDKPSSKSEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSMEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDDVNPENQVIQQNEPSESTPPGSSKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDEEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDSLDKDSDLVKYISDLTISTKPHQVKMPTVIECSVRKQNVQFMHNRMQYSLEYFQFIKKLLTCNSVYLNPPPGQDHLLPEAEEIAMISTQLAARFLFSTGFHTKKVVRGPASDWYDALCILLRHSKNVRYWFAHNVLFAYPNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPTASPGPSAQGCDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGLAEKSQLLKLNVPATFMLVALDEGPGPPIKYQYAELGKLYTVVSQLVRCCEVSSRMQSSINGNPPLPNPYGDPNLTAPVMSVQQLVAEILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNVLKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCSVAYQILQSNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEEEPDEQEAPDDQDSSPPEDTSLYPHSPGSTQFQQNNHPHGQPYTGPAAQHMNNPQRPGPASAPTPGPAQTPASGPGTAPGSGPRAQEHWEGTEEVAPAPASTPAPVPPKE

Target ORF information:

RefSeq Version	XM_023950531.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ubiquitin specific peptidase 9, X-linked (usp9x), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023950531.1

ORF Insert Sequence:

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGCCAA ACCCAGAGAA GTCCACACGT GTTTTACCGG 
CACGACCTGA TCAACCAGCT GCAGCATAAC CACGCTCTGG TCACCCTCGT GGCCGAGAAC 
CTCTCTGCTT ACATGGAGAC TATGAGGCAG CTATCCAAAG AACAAGCAGA GTTTGATCCT 
CAGACAGTCA GACCAGGAAG CCGCTACAGT CACGTCCAAG AAGTTCAGGA GCGACTCAAC 
TTCCTGAGGT TTCTTTTAAA AGACGGCCAG CTGTGGCTGT GTGCCCCTCA AGCCAAGCAG 
ATCTGGAAGT GTCTGGCTGA GAATGCTGTG TTTCTCTGCG ATCGCGAAGC TTGCTTCAAA 
TGGTACTCCA AGCTGATGGG GGATGAACCA GACCTGGATC CGGACATCAA TAAGGACTTC 
TTTGAGAATA ACGTTCTGCA GTTGGATCCG TCTCTGCTGA CGGAAAATGG CATGAAGTGC 
TTTGAGAGGT TCTTCAAGGC CGTCAACTGC AGGGAGGGGA AGCTGGTTGC AAAGCGCAGG 
GCTTACATGA TGGATGACTT GGAGCTAATA GGCCTGGACT ATCTGTGGAG GGTGGTAATT 
CAAGGAAGCG ATGACATTGC TAGCCGAGCC ATAGACCTGC TGAAAGAGAT TTACACCAAC 
CTTGGTCCCA AACTACAAGT CAATCAGGTT GAGATTCACG AGGATTTCAT CCAGTCCTGC 
TTCGACCGTC TGAAGGCGTC TTATGATACC CTTTGTGTTT TGGACGGAGA CAAAGACAGC 
ATTAACTGTG CTAGGCAGGA GGCCATCCGC ATGGTACGCG TTCTGACTGT GCTCAAGGAG 
TATATCAACG AGTGTGACAG CGACTACCAC GAGGAGAGAA CCATACTGCC CATGTCCAGA 
GCTTTCAGGG GAAAGCACAT AACTTTGATT GTGCGTTTCC CAAACCAAGG TCGCCAAGTA 
GATGATTTAG ATATCTGGTC CCACACCAAC GACACGATTG GCTCGGTGAG GCGGGCGATT 
CTAAACAGAA TAAAGGCTAA TGCTGCGCAT ACAAAGGTCG AGTTGTTTAT TGGGGGTGAG 
GTGGTGGATC CAGCTGATGA CAGGAAGTTG ATCGGGCAGC TCAGTCTGAA GGACAAAACG 
TTGATCACGG CCAAGCTGAC CCAGGTGAGT GCCAACATGC CCTCAAGTCC AGACAGCTCC 
TCTGACTCGT CTACTGGCTC TCCTGGTAAC CATGGAAACC ACTACAGCGA CGGGCCCAAC 
CCTGAGGTGG AAAGCTGTCT GCCTGGCGTG ATTATGTCGC TGCATCTGCG GTACATCTCC 
TTCCTGTGGC AGATCGCTGA TCTAGGCTGC AGCCTCAACA TGCCTCTACT TAGAGATGGA 
GCTCGTGTTC TCATGAAGCT TATGCCTCCT GATAACACCA CAGTAGAGAA TCTGAGAGCT 
GTGTGTTTGG ACCATGCCAA GCTGGGAGAG AACAGCCTGA GTCCCTCACT GGACTCCTGC 
TTCTTTGGCC CCTCCCCCTC ACAAGTGCTC TACCTCATAG AGGTCGTGTA TGCTTTGCTG 
ATGCCAGCCA GTGCCACTCT CGGTGAAGAC GCCAGCGACT TCCAATACAA CTTCCTGAAG 
AGCGGCGGCC TGCCCTTGGT GCTGAGCATG CTCACCAGAA ACAACTTTCT CCCATCGGCA 
GACATGGAAA CTCGAAGGGG CGCCTATGTC AACGCACTAA AGATCGCAAA GCTCCTACTG 
ACCGCTGTGG GTTTTGGACA CGTGAAGGCG GTAGCTGAGG CCTGCCAGCC AAACGCCGAC 
GGAAATATTC CCGTCTCTCC AATTAATCAG GCAACGCACG ACCAAGCGCT GGTTCTTCAG 
AGCGCCCTGC AAAGCATCCC TAACCCGGCC TCAGAGTGCA TGCTGCGTAA TGTAGCCATT 
CGCCTGGCCC AACAGATCTC TGACGAGAAC TCTATTTGCC ATCAGAACTT TTTCCAGGCG 
TCGAAGTACA TCCCAGACAT TTGTGTAATC CGAGCAATAC AGAAAATCGT GTGGGCATCA 
GGCTGTGGTA CAGTGCAGCT CGTCTTTAGC TCCAATGAGG AGATCAGCAG GATATATGAG 
AAGACAAATG CTGCCAAGGA ACCAGATGGA GAGGATGAGC AGGTGTGCTG CGAGGCCTTG 
GAGGTCATGA CCCTGTGCTT TGCCCTCATG CCCACGGCTC TGGACACACT AAGCAAAGAG 
AAGGCGTGGC AGACTTTTAT CATAGACCTG TTACTACACT GCCACAGCAA GTCCGTTCGA 
CAAATGGCTC AGGAGCAGTT TTTCCTCATG GCCACAAGAT GCTGCATGGG CCACCGACCC 
CTCCTCTTCT TCATCACCCT GTTATTCACT GTGTTGGGGA GCACGGCCAA AGAGAGAGCC 
AAACATGCGG GGGACTACTT CACCTTGCTC AGACACCTGC TAAACTATGC TTACAACAGC 
AACATCAACC TGCCAAACGC CGAGGTGCTG CTTAACAATG AGATTGACTG GCTCAAGAGA 
ATACGGGATG AGGTGAAGAG AACAGGAGAG ACTGGTGTGG AGGAGACGAT CCTGGAGGGC 
CACCTTGGAG TCACCAAAGA GCTCCTTGCT TTTCAAACAC CGGAAAAGAA GTACTACATT 
GGCAGCGAGA AGGGAGGAGC CAACCTTATA AAGGAGCTGA TCGATGACTT CATATTCCCA 
GCATCAAATG TTTACCTGCA GTACATGAAG ACGGGAGAGT TCCCCACAGA GCAGGCCATC 
CCAGTCTGCA GCACTCCCGC CTCCATCAAT GCTGGCTTTG AGCTCCTGGT GGCTCTGGCC 
GTTGGCTGCA TGCGCAATCT GAAACAAATA GTTGACACCC TGACTGATAT GTACTACTCA 
GGCGGGGAGA CGTTGACAGA GTGGGAGTAC TTGCCTCCAG TTGGGCCACG ACCCAATAAA 
GGCTTTGTAG GTCTAAAGAA CGCCGGTGCC ACATGTTACA TGAACTCTGT CATTCAGCAA 
CTGTACATGA TCCCTCCCAT TCGCAACGGC ATTCTGGCTA TTGAGGGAAC AGGCACGGAT 
GTGGACGATG ACATGTCGGG GGATGAGAAG CAGGAGAATG AGAGTAACGT CGATCCTCGT 
GATGAGGTCT TCAGCTACCA TCACCAGTTT GATGACAAAC CGTCCAGTAA GTCAGAGGAC 
AGGAAGGAGT ACAACATCGG CGTGCTTCGT CACTTGCAGG TCATCTTTGG TCATCTTGCT 
GCATCCAGGC TGCAGTACTA CGTCCCAAGA GGCTTCTGGA AACAGTTTAG ATTGTGGGGT 
GAGCCAGTCA ACCTGAGGGA ACAGCACGAT GCTCTGGAGT TTTTCAACTC TTTGGTGGAC 
AGTTTGGATG AAGCTCTGAA GGCTCTGGGT CACCCCGCCA TGCTCAGTAA GGTGCTGGGA 
GGCTCTTTCG CCGATCAGAA AATCTGTCAA GGGTGCCCTC ACAGGTATGA GTGTGAGGAG 
TCGTTTACAA CACTTAACGT GGACATCAGA AACCACCAAA ACCTGTTGGA CTCCATGGAG 
CAGTATGTCA AAGGAGATCT GCTGGAAGGA GCAAATGCCT ATCACTGTGA GAAGTGTAAT 
AAAAAGGTGG ACACCGTGAA GCGTCTGTTG ATCAAGAAGC TCCCACCCGT CCTGGCCATT 
CAGCTGAAGC GCTTTGACTA CGACTGGGAG CGAGAATGTG CCATCAAGTT TAACGACTAC 
TTTGAGTTCC CCAGGGAGCT GGATATGGAG CCTTACACGG TAGCGGGCGT GGCCAAACTG 
GAGGGCGACG ACGTGAACCC GGAGAACCAG GTCATACAGC AGAACGAGCC GTCTGAATCC 
ACACCGCCGG GCAGCTCCAA ATATCGTCTG GTGGGAGTGC TGGTCCACTC AGGCCAGGCC 
AGTGGCGGAC ACTACTATTC CTACATAATC CAGAGGAACG GAGGGGACGG GGAGAAGAAC 
CGCTGGTATA AGTTCGACGA CGGCGACGTC ACTGAATGCA AGATGGACGA CGAGGAGGAG 
ATGAAGAACC AGTGCTTCGG TGGGGAGTAC ATGGGCGAGG TGTTTGATCA TATGATGAAA 
AGGATGTCGT ATCGGAGGCA AAAGCGCTGG TGGAACGCTT ACATCCTGTT TTACGAGCGA 
ATGGACTCGT TGGACAAGGA CAGCGATCTC GTCAAATACA TCTCGGACCT GACGATCTCC 
ACCAAGCCAC ATCAGGTCAA GATGCCCACG GTCATCGAGT GCAGCGTCCG CAAGCAGAAC 
GTCCAGTTCA TGCACAACCG AATGCAATAC AGCCTAGAAT ATTTCCAGTT CATAAAGAAA 
CTTCTGACCT GTAACAGTGT CTATTTAAAC CCTCCTCCAG GGCAAGACCA CCTGCTGCCA 
GAGGCAGAGG AGATCGCGAT GATCAGCACT CAGCTGGCTG CTAGGTTCCT CTTCAGCACA 
GGTTTCCACA CCAAGAAAGT AGTACGGGGT CCTGCCAGTG ACTGGTATGA CGCCCTCTGC 
ATCCTGCTGA GACACAGTAA GAATGTTCGT TACTGGTTTG CACACAACGT CCTGTTTGCG 
TACCCTAACC GCTTCTCCGA GTATTTGCTG GAGTGCCCCA GCGCTGAGGT CCGCGGAGCA 
TTTGCCAAGC TCATCGTCTT CATCGCTCAC TTCTCCCTGC AAGATGGCCC CTGCCCCTCC 
CCCACCGCCT CACCTGGACC GTCTGCTCAG GGCTGTGATA ATCTCAGTTT AAGTGACCAC 
TTGTTGAGAG CTGTTCTAAA CCTGCTTAGG AGAGAGGTTT CTGAACATGG CCGACATCTA 
CAGCAGTACT TCAACCTCTT TGTCATGTAT GCCAATCTGG GCCTGGCAGA GAAGTCTCAG 
CTGCTGAAGC TCAACGTTCC CGCCACTTTC ATGCTGGTCG CTCTAGACGA GGGTCCCGGT 
CCCCCCATCA AATACCAGTA TGCAGAACTG GGCAAGCTCT ACACTGTGGT TTCCCAGCTG 
GTCCGCTGCT GTGAGGTGTC CTCGCGCATG CAATCCTCCA TCAATGGTAA CCCTCCTCTT 
CCTAACCCGT ATGGGGATCC CAACCTGACG GCTCCGGTGA TGTCTGTGCA GCAGCTGGTG 
GCAGAAATCC TGTTTGTGAG GACGAGCTAC GTCAAGAAGA TCATCGAGGA CTGCAGCAAC 
TCTGAGGAGA CCGTGAAGCT CCTTAGATTC TGCTGCTGGG AGAATCCTCA GTTCTCCTCC 
ACTGTGCTCA GTGAACTGCT TTGGCAGGTG GCATATTCTT ACACGTATGA GCTGAGGCCT 
TACCTGGACT TGCTGCTACA GATCCTTCTC ATCGAAGACT CCTGGCAGAC GCACAGGATC 
CACAATGTCC TAAAGGGCAT CCCTGATGAC AGAGACGGGC TGTTTGACAC CATCCAGCGC 
TCCAAAAACC ATTACCAGAA GAGGGCCTAC CAGTGCATCA AGTGCATGGT GGCCCTCTTT 
AGCAACTGCT CCGTGGCTTA CCAGATCCTG CAGAGTAATG GTGACCTGAA AAGGAAGTGG 
ACGTGGGCGG TGGAGTGGTT GGGGGATGAG TTGGAGAGAA GGCCGTACAC AGGAAACCCG 
CAGTACACCT ACAACAACTG GTCCCCCCCA GTTCAGAGCA ACGAGACCTC CAACGGGTAT 
TTCCTGGAGC GTTCACACAG TGCACGTATG ACACTTGCCA AAGCCTGTGA ACTCTGTCCT 
GAAGAGGAGC CGGACGAACA GGAAGCACCT GATGATCAAG ACTCCTCCCC TCCTGAAGAC 
ACCTCTCTGT ACCCACATTC TCCTGGAAGC ACCCAGTTTC AGCAGAATAA CCACCCCCAT 
GGGCAGCCTT ACACCGGGCC TGCTGCCCAG CACATGAACA ACCCCCAGCG CCCCGGCCCA 
GCCTCCGCTC CGACTCCCGG CCCGGCCCAG ACCCCCGCCT CAGGCCCCGG CACCGCCCCT 
GGTTCCGGCC CGCGAGCACA AGAGCACTGG GAGGGCACAG AGGAAGTCGC CCCCGCCCCC 
GCCTCCACCC CAGCACCTGT CCCCCCGAAG GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp9x ( XM_011489289.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011487591.2 Oryzias latipes probable ubiquitin carboxyl-terminal hydrolase FAF-X (LOC101166345), mRNA.

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CloneID	OJa75229
Clone ID Related Accession (Same CDS sequence)	XM_011489289.2
Accession Version	XM_011489289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	LOW QUALITY PROTEIN: probable ubiquitin carboxyl-terminal hydrolase FAF-X
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011489289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGCCAA ACCCAGAGAA GTCCACACGT GTTTTACCGG 
CACGACCTGA TCAACCAGCT GCAGCATAAC CACGCTCTGG TCACCCTCGT GGCCGAGAAC 
CTCTCTGCTT ACATGGAGAC TATGAGGCAG CTATCCAAAG AAGAACAAGC AGAGTTTGAT 
CCTCAGACAG TCAGACCAGG AAGCCGCTAC AGTCACGTCC AAGAAGTTCA GGAGCGACTC 
AACTTCCTGA GGTTTCTTTT AAAAGACGGC CAGCTGTGGC TGTGTGCCCC TCAAGCCAAG 
CAGATCTGGA AGTGTCTGGC TGAGAATGCT GTGTTTCTCT GCGATCGCGA AGCTTGCTTC 
AAATGGTACT CCAAGCTGAT GGGGGATGAA CCAGACCTGG ATCCGGACAT CAATAAGGAC 
TTCTTTGAGA ATAACGTTCT GCAGTTGGAT CCGTCTCTGC TGACGGAAAA TGGCATGAAG 
TGCTTTGAGA GGTTCTTCAA GGCCGTCAAC TGCAGGGAGG GGAAGCTGGT TGCAAAGCGC 
AGGGCTTACA TGATGGATGA CTTGGAGCTA ATAGGCCTGG ACTATCTGTG GAGGGTGGTA 
ATTCAAGGAA GCGATGACAT TGCTAGCCGA GCCATAGACC TGCTGAAAGA GATTTACACC 
AACCTTGGTC CCAAACTACA AGTCAATCAG GTTGAGATTC ACGAGGATTT CATCCAGTCC 
TGCTTCGACC GTCTGAAGGC GTCTTATGAT ACCCTTTGTN GTTTGGACGG AGACAAAGAC 
AGCATTAACT GTGCTAGGCA GGAGGCCATC CGCATGGTAC GCGTTCTGAC TGTGCTCAAG 
GAGTATATCA ACGAGTGTGA CAGCGACTAC CACGAGGAGA GAACCATACT GCCCATGTCC 
AGAGCTTTCA GGGGAAAGCA CATAACTTTG ATTGTGCGTT TCCCAAACCA AGGTCGCCAA 
GTAGATGATT TAGATATCTG GTCCCACACC AACGACACGA TTGGCTCGGT GAGGCGGGCG 
ATTCTAAACA GAATAAAGGC TAATGCTGCG CATACAAAGG TCGAGTTGTT TATTGGGGGT 
GAGGTGGTGG ATCCAGCTGA TGACAGGAAG TTGATCGGGC AGCTCAGTCT GAAGGACAAA 
ACGTTGATCA CGGCCAAGCT GACCCAGGTG AGTGCCAACA TGCCCTCAAG TCCAGACAGC 
TCCTCTGACT CGTCTACTGG CTCTCCTGGT AACCATGGAA ACCACTACAG CGACGGGCCC 
AACCCTGAGG TGGAAAGCTG TCTGCCTGGC GTGATTATGT CGCTGCATCT GCGGTACATC 
TCCTTCCTGT GGCAGATCGC TGATCTAGGC TGCAGCCTCA ACATGCCTCT ACTTAGAGAT 
GGAGCTCGTG TTCTCATGAA GCTTATGCCT CCTGATAACA CCACAGTAGA GAATCTGAGA 
GCTGTGTGTT TGGACCATGC CAAGCTGGGA GAGAACAGCC TGAGTCCCTC ACTGGACTCC 
TGCTTCTTTG GCCCCTCCCC CTCACAAGTG CTCTACCTCA TAGAGGTCGT GTATGCTTTG 
CTGATGCCAG CCAGTGCCAC TCTCGGTGAA GACGCCAGCG ACTTCCAATA CAACTTCCTG 
AAGAGCGGCG GCCTGCCCTT GGTGCTGAGC ATGCTCACCA GAAACAACTT TCTCCCATCG 
GCAGACATGG AAACTCGAAG GGGCGCCTAT GTCAACGCAC TAAAGATCGC AAAGCTCCTA 
CTGACCGCTG TGGGTTTTGG ACACGTGAAG GCGGTAGCTG AGGCCTGCCA GCCAAACGCC 
GACGGAAATA TTCCCGTCTC TCCAATTAAT CAGGCAACGC ACGACCAAGC GCTGGTTCTT 
CAGAGCGCCC TGCAAAGCAT CCCTAACCCG GCCTCAGAGT GCATGCTGCG TAATGTAGCC 
ATTCGCCTGG CCCAACAGAT CTCTGACGAG GTAACGCTTC TTGTNNNNNN NATTTGTGTA 
ATCCGAGCAA TACAGAAAAT CGTGTGGGCA TCAGGCTGTG GTACAGTGCA GCTCGTCTTT 
AGCTCCAATG AGGAGATCAG CAGGATATAT GAGAAGACAA ATGCTGCCAA GGAACCAGAT 
GGAGAGGATG AGCAGGTGTG CTGCGAGGCC TTGGAGGTCA TGACCCTGTG CTTTGCCCTC 
ATGCCCACGG CTCTGGACAC ACTAAGCAAA GAGAAGGCGT GGCAGACTTT TATCATAGAC 
CTGTTACTAC ACTGCCACAG CAAGTCCGTT CGACAAATGG CTCAGGAGCA GTTTTTCCTC 
ATGGCCACAA GATGCTGCAT GGGCCACCGA CCCCTCCTCT TCTTCATCAC CCTGTTATTC 
ACTGTGTTGG GGAGCACGGC CAAAGAGAGA GCCAAACATG CGGGGGACTA CTTCACCTTG 
CTCAGACACC TGCTAAACTA TGCTTACAAC AGCAACATCA ACCTGCCAAA CGCCGAGGTG 
CTGCTTAACA ATGAGATTGA CTGGCTCAAG AGAATACGGG ATGAGGTGAA GAGAACAGGA 
GAGACTGGTG TGGAGGAGAC GATCCTGGAG GGCCACCTTG GAGTCACCAA AGAGCTCCTT 
GCTTTTCAAA CACCGGAAAA GAAGTACTAC ATTGGCAGCG AGAAGGGAGG AGCCAACCTT 
ATAAAGGAGC TGATCGATGA CTTCATATTC CCAGCATCAA ATGTTTACCT GCAGTACATG 
AAGACGGGAG AGTTCCCCAC AGAGCAGGCC ATCCCAGTCT GCAGCACTCC CGCCTCCATC 
AATGCTGGCT TTGAGCTCCT GGTGGCTCTG GCCGTTGGCT GCATGCGCAA TCTGAAACAA 
ATAGTTGACA CCCTGACTGA TATGTACTAC TCAGGCGGGG AGACGTTGAC AGAGTGGGAG 
TACTTGCCTC CAGTTGGGCC ACGACCCAAT AAAGGCTTTG TAGGTCTAAA GAACGCCGGT 
GCCACATGTT ACATGAACTC TGTCATTCAG CAACTGTACA TGATCCCTCC CATTCGCAAC 
GGCATTCTGG CTATTGAGGG AACAGGCACG GATGTGGACG ATGACATGTC GGGGGATGAG 
AAGCAGGAGA ATGAGAGTAA CGTCGATCCT CGTGATGAGG TCTTCAGCTA CCATCACCAG 
TTTGATGACA AACCGTCCAG TAAGTCAGAG GACAGGAAGG AGTACAACAT CGGCGTGCTT 
CGTCACTTGC AGGTCATCTT TGGTCATCTT GCTGCATCCA GGCTGCAGTA CTACGTCCCA 
AGAGGCTTCT GGAAACAGTT TAGATTGTGG GGTGAGCCAG TCAACCTGAG GGAACAGCAC 
GATGCTCTGG AGTTTTTCAA CTCTTTGGTG GACAGTTTGG ATGAAGCTCT GAAGGCTCTG 
GGTCACCCCG CCATGCTCAG TAAGGTGCTG GGAGGCTCTT TCGCCGATCA GAAAATCTGT 
CAAGGGTGCC CTCACAGGTA TGAGTGTGAG GAGTCGTTTA CAACACTTAA CGTGGACATC 
AGAAACCACC AAAACCTGTT GGACTCCATG GAGCAGTATG TCAAAGGAGA TCTGCTGGAA 
GGAGCAAATG CCTATCACTG TGAGAAGTGT AATAAAAAGG TGGACACCGT GAAGCGTCTG 
TTGATCAAGA AGCTCCCACC CGTCCTGGCC ATTCAGCTGA AGCGCTTTGA CTACGACTGG 
GAGCGAGAAT GTGCCATCAA GTTTAACGAC TACTTTGAGT TCCCCAGGGA GCTGGATATG 
GAGCCTTACA CGGTAGCGGG CGTGGCCAAA CTGGAGGGCG ACGACGTGAA CCCGGAGAAC 
CAGGTCATAC AGCAGAACGA GCCGTCTGAA TCCACACCGC CGGGCAGCTC CAAATATCGT 
CTGGTGGGAG TGCTGGTCCA CTCAGGCCAG GCCAGTGGCG GACACTACTA TTCCTACATA 
ATCCAGAGGA ACGGAGGGGA CGGGGAGAAG AACCGCTGGT ATAAGTTCGA CGACGGCGAC 
GTCACTGAAT GCAAGATGGA CGACGAGGAG GAGATGAAGA ACCAGTGCTT CGGTGGGGAG 
TACATGGGCG AGGTGTTTGA TCATATGATG AAAAGGATGT CGTATCGGAG GCAAAAGCGC 
TGGTGGAACG CTTACATCCT GTTTTACGAG CGAATGGACT CGTTGGACAA GGACAGCGAT 
CTCGTCAAAT ACATCTCGGA CCTGACGATC TCCACCAAGC CACATCAGGT CAAGATGCCC 
ACGGTCATCG AGTGCAGCGT CCGCAAGCAG AACGTCCAGT TCATGCACAA CCGAATGCAA 
TACAGCCTAG AATATTTCCA GTTCATAAAG AAACTTCTGA CCTGTAACAG TGTCTATTTA 
AACCCTCCTC CAGGGCAAGA CCACCTGCTG CCAGAGGCAG AGGAGATCGC GATGATCAGC 
ACTCAGCTGG CTGCTAGGTT CCTCTTCAGC ACAGGTTTCC ACACCAAGAA AGTAGTACGG 
GGTCCTGCCA GTGACTGGTA TGACGCCCTC TGCATCCTGC TGAGACACAG TAAGAATGTT 
CGTTACTGGT TTGCACACAA CGTCCTGTTT GCGTACCCTA ACCGCTTCTC CGAGTATTTG 
CTGGAGTGCC CCAGCGCTGA GGTCCGCGGA GCATTTGCCA AGCTCATCGT CTTCATCGCT 
CACTTCTCCC TGCAAGATGG CCCCTGCCCC TCCCCCACCG CCTCACCTGG ACCGTCTGCT 
CAGGGCTGTG ATAATCTCAG TTTAAGTGAC CACTTGTTGA GAGCTGTTCT AAACCTGCTT 
AGGAGAGAGG TTTCTGAACA TGGCCGACAT CTACAGCAGT ACTTCAACCT CTTTGTCATG 
TATGCCAATC TGGGCCTGGC AGAGAAGTCT CAGCTGCTGA AGCTCAACGT TCCCGCCACT 
TTCATGCTGG TCGCTCTAGA CGAGGGTCCC GGTCCCCCCA TCAAATACCA GTATGCAGAA 
CTGGGCAAGC TCTACACTGT GGTTTCCCAG CTGGTCCGCT GCTGTGAGGT GTCCTCGCGC 
ATGCAATCCT CCATCAATGG TAACCCTCCT CTTCCTAACC CGTATGGGGA TCCCAACCTG 
ACGGCTCCGG TGATGTCTGT GCAGCAGCTG GTGGCAGAAA TCCTGTTTGT GAGGACGAGC 
TACGTCAAGA AGATCATCGA GGACTGCAGC AACTCTGAGG AGACCGTGAA GCTCCTTAGA 
TTCTGCTGCT GGGAGAATCC TCAGTTCTCC TCCACTGTGC TCAGTGAACT GCTTTGGCAG 
GTGGCATATT CTTACACGTA TGAGCTGAGG CCTTACCTGG ACTTGCTGCT ACAGATCCTT 
CTCATCGAAG ACTCCTGGCA GACGCACAGG ATCCACAATG TCCTAAAGGG CATCCCTGAT 
GACAGAGACG GGCTGTTTGA CACCATCCAG CGCTCCAAAA ACCATTACCA GAAGAGGGCC 
TACCAGTGCA TCAAGTGCAT GGTGGCCCTC TTTAGCAACT GCTCCGTGGC TTACCAGATC 
CTGCAGAGTA ATGGTGACCT GAAAAGGAAG TGGACGTGGG CGGTGGAGTG GTTGGGGGAT 
GAGTTGGAGA GAAGGCCGTA CACAGGAAAC CCGCAGTACA CCTACAACAA CTGGTCCCCC 
CCAGTTCAGA GCAACGAGAC CTCCAACGGG TATTTCCTGG AGCGTTCACA CAGTGCACGT 
ATGACACTTG CCAAAGCCTG TGAACTCTGT CCTGAAGAGG AGCCGGACGA ACAGGAAGCA 
CCTGATGATC AAGACTCCTC CCCTCCTGAA GACACCTCTC TGTACCCACA TTCTCCTGGA 
AGCACCCAGT TTCAGCAGGT ACTGCAGGAA AAACTGCATC ATAAAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011487591.2
CDS	676..8226
Translation	MTATTRGSPVGGNDSQGQGQAPDAQSQPPLPQNQTSSPNSSNENSPVSPPDEQSQVDRPPQLEEEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKCEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMATNPHCKFHIYNGTRPSETVPAGAQLPDDELYARPPDPRSPKGWLVDLINKFGNLNGFQILHDRFMSGQALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPVIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHNPEEEEWLTAERMAEWIQQNHILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTMQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDQALNAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLSAYMETMRQLSKEEQAEFDPQTVRPGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVFLCDREACFKWYSKLMGDEPDLDPDINKDFFENNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQGSDDIASRAIDLLKEIYTNLGPKLQVNQVEIHEDFIQSCFDRLKASYDTLCnullLDGDKDSINCARQEAIRMVRVLTVLKEYINECDSDYHEERTILPMSRAFRGKHITLIVRFPNQGRQVDDLDIWSHTNDTIGSVRRAILNRIKANAAHTKVELFIGGEVVDPADDRKLIGQLSLKDKTLITAKLTQVSANMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHLRYISFLWQIADLGCSLNMPLLRDGARVLMKLMPPDNTTVENLRAVCLDHAKLGENSLSPSLDSCFFGPSPSQVLYLIEVVYALLMPASATLGEDASDFQYNFLKSGGLPLVLSMLTRNNFLPSADMETRRGAYVNALKIAKLLLTAVGFGHVKAVAEACQPNADGNIPVSPINQATHDQALVLQSALQSIPNPASECMLRNVAIRLAQQISDEVTLLnullnullnullICVIRAIQKIVWASGCGTVQLVFSSNEEISRIYEKTNAAKEPDGEDEQVCCEALEVMTLCFALMPTALDTLSKEKAWQTFIIDLLLHCHSKSVRQMAQEQFFLMATRCCMGHRPLLFFITLLFTVLGSTAKERAKHAGDYFTLLRHLLNYAYNSNINLPNAEVLLNNEIDWLKRIRDEVKRTGETGVEETILEGHLGVTKELLAFQTPEKKYYIGSEKGGANLIKELIDDFIFPASNVYLQYMKTGEFPTEQAIPVCSTPASINAGFELLVALAVGCMRNLKQIVDTLTDMYYSGGETLTEWEYLPPVGPRPNKGFVGLKNAGATCYMNSVIQQLYMIPPIRNGILAIEGTGTDVDDDMSGDEKQENESNVDPRDEVFSYHHQFDDKPSSKSEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSMEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDDVNPENQVIQQNEPSESTPPGSSKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDEEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDSLDKDSDLVKYISDLTISTKPHQVKMPTVIECSVRKQNVQFMHNRMQYSLEYFQFIKKLLTCNSVYLNPPPGQDHLLPEAEEIAMISTQLAARFLFSTGFHTKKVVRGPASDWYDALCILLRHSKNVRYWFAHNVLFAYPNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPTASPGPSAQGCDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGLAEKSQLLKLNVPATFMLVALDEGPGPPIKYQYAELGKLYTVVSQLVRCCEVSSRMQSSINGNPPLPNPYGDPNLTAPVMSVQQLVAEILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNVLKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCSVAYQILQSNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEEEPDEQEAPDDQDSSPPEDTSLYPHSPGSTQFQQVLQEKLHHKI

Target ORF information:

RefSeq Version	XM_011489289.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes probable ubiquitin carboxyl-terminal hydrolase FAF-X (LOC101166345), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011489289.2

ORF Insert Sequence:

ATGACGGCCA CCACGCGTGG CTCTCCAGTG GGGGGCAATG ACAGCCAGGG CCAGGGCCAG 
GCACCTGATG CTCAAAGCCA GCCCCCACTG CCACAGAACC AGACATCCTC TCCTAACTCA 
TCGAATGAGA ACTCCCCAGT GAGCCCACCA GATGAGCAGA GTCAGGTTGA TAGGCCCCCT 
CAGCTGGAGG AGGAAGAGCC TGCTTTCCCT CACACTGACC TAGCCAAGCT CGATGACATG 
ATCAACAGGC CTCGTTGGGT TGTTCCAGTT TTGCCAAAAG GAGAATTAGA AGTTCTCTTG 
GAAGCTGCTA TAGACCTGAG TAAAAAAGGG CTGGACGTGA AGTGTGAGGC TTGTCAGAGG 
TTTTTCAGGG ATGGTCTGAC CATTTCCTTC ACAAAGATCC TGACAGACGA GGCAGTCAGT 
GGCTGGAAGT TTGAGATTCA TAGATGTATC ATTAATAACA CACACCGTTT GGTGGAGCTG 
TGTGTTGCAA AGCTCTCTCA GGACTGGTTC CCTTTATTGG AGCTGTTAGC CATGGCCACC 
AACCCCCACT GCAAGTTCCA CATTTACAAT GGCACACGGC CCTCGGAGAC CGTCCCTGCT 
GGCGCACAGC TGCCTGATGA TGAGCTCTAT GCCCGACCAC CAGACCCGCG ATCTCCTAAG 
GGTTGGTTGG TGGACTTAAT AAACAAATTT GGCAATTTAA ACGGGTTTCA AATATTGCAC 
GATCGCTTCA TGAGCGGCCA AGCACTGAAC GTCCAGATCA TCGCTGCACT TATCAAACCT 
TTTGGCCAGT GTTACGAGTT CCTCACATTG CACACAGTAA AGAAGTACTT CCTTCCAGTC 
ATCGAGATGG TTCCTCAGTT TCTAGAGAAT CTCACGGATG AGGAGCTTAA AAAAGAGGCC 
AAGAACGAAG CCAAAAATGA CGCACTGTCC ATGATAATCA AGTCTCTGAA GAATCTGGCC 
TCTCGTGTAC CAGGACAGGA GGAGACTGTG AAGAATTTAG AGATTTTTAG GTTAAAAATG 
ATTCTTAGGT TATTGCAAAT TTCTTCTTTT AATGGCAAAA TGAATGCACT AAATGAAGTT 
AACAAAGTGA TCTCCAGCGT GTCCTACTAC ACTCATCGTC ATAACCCCGA GGAAGAGGAA 
TGGCTGACTG CTGAGCGGAT GGCGGAATGG ATCCAGCAAA ACCACATACT GTCTATCGTG 
CTAAGAGACA GTTTGCACCA ACCCCAGTAT GTGGAAAAAC TGGAGAAGAT TCTTCGCTTT 
GTTATTAAGG AGAAAGCTCT TACCATGCAA GATTTGGACA ACATCTGGGC AGCACAGGCT 
GGAAAGCATG AGGCTATTGT AAAGAATGTC CATGACCTTC TGGCTAAGCT AGCATGGGAC 
TTCTCACCTG AGCAGCTTGA TCATCTTTTT GACTGTTTTA AGGCGAGCTG GACCAATGCC 
AGCAAGAAGC AGCGTGAAAA GCTGCTGGAA CTTATCCGGC GCTTAGCAGA GGACGACAAA 
GACGGGGTGA TGGCCCACAA GGTCCTCAAC CTGCTGTGGA ACCTGGCGCA CAGTGATGAC 
GTTCCTGTAG ATATTATGGA CCAGGCACTC AATGCTCACA TCAAAATATT AGACTACAGT 
TGCTCACAGG ACAGAGACAC GCAGAAGATT CAATGGATCG ATCGTTTTAT AGAGGAGCTG 
CGAACCAATG ACAAATGGGT GATCCCTGCC TTGAAACAAA TCAGAGAAAT CTGCAGCCTC 
TTTGGTGAAG CTCCTCAAAA TCTTAGCCAA ACCCAGAGAA GTCCACACGT GTTTTACCGG 
CACGACCTGA TCAACCAGCT GCAGCATAAC CACGCTCTGG TCACCCTCGT GGCCGAGAAC 
CTCTCTGCTT ACATGGAGAC TATGAGGCAG CTATCCAAAG AAGAACAAGC AGAGTTTGAT 
CCTCAGACAG TCAGACCAGG AAGCCGCTAC AGTCACGTCC AAGAAGTTCA GGAGCGACTC 
AACTTCCTGA GGTTTCTTTT AAAAGACGGC CAGCTGTGGC TGTGTGCCCC TCAAGCCAAG 
CAGATCTGGA AGTGTCTGGC TGAGAATGCT GTGTTTCTCT GCGATCGCGA AGCTTGCTTC 
AAATGGTACT CCAAGCTGAT GGGGGATGAA CCAGACCTGG ATCCGGACAT CAATAAGGAC 
TTCTTTGAGA ATAACGTTCT GCAGTTGGAT CCGTCTCTGC TGACGGAAAA TGGCATGAAG 
TGCTTTGAGA GGTTCTTCAA GGCCGTCAAC TGCAGGGAGG GGAAGCTGGT TGCAAAGCGC 
AGGGCTTACA TGATGGATGA CTTGGAGCTA ATAGGCCTGG ACTATCTGTG GAGGGTGGTA 
ATTCAAGGAA GCGATGACAT TGCTAGCCGA GCCATAGACC TGCTGAAAGA GATTTACACC 
AACCTTGGTC CCAAACTACA AGTCAATCAG GTTGAGATTC ACGAGGATTT CATCCAGTCC 
TGCTTCGACC GTCTGAAGGC GTCTTATGAT ACCCTTTGTN GTTTGGACGG AGACAAAGAC 
AGCATTAACT GTGCTAGGCA GGAGGCCATC CGCATGGTAC GCGTTCTGAC TGTGCTCAAG 
GAGTATATCA ACGAGTGTGA CAGCGACTAC CACGAGGAGA GAACCATACT GCCCATGTCC 
AGAGCTTTCA GGGGAAAGCA CATAACTTTG ATTGTGCGTT TCCCAAACCA AGGTCGCCAA 
GTAGATGATT TAGATATCTG GTCCCACACC AACGACACGA TTGGCTCGGT GAGGCGGGCG 
ATTCTAAACA GAATAAAGGC TAATGCTGCG CATACAAAGG TCGAGTTGTT TATTGGGGGT 
GAGGTGGTGG ATCCAGCTGA TGACAGGAAG TTGATCGGGC AGCTCAGTCT GAAGGACAAA 
ACGTTGATCA CGGCCAAGCT GACCCAGGTG AGTGCCAACA TGCCCTCAAG TCCAGACAGC 
TCCTCTGACT CGTCTACTGG CTCTCCTGGT AACCATGGAA ACCACTACAG CGACGGGCCC 
AACCCTGAGG TGGAAAGCTG TCTGCCTGGC GTGATTATGT CGCTGCATCT GCGGTACATC 
TCCTTCCTGT GGCAGATCGC TGATCTAGGC TGCAGCCTCA ACATGCCTCT ACTTAGAGAT 
GGAGCTCGTG TTCTCATGAA GCTTATGCCT CCTGATAACA CCACAGTAGA GAATCTGAGA 
GCTGTGTGTT TGGACCATGC CAAGCTGGGA GAGAACAGCC TGAGTCCCTC ACTGGACTCC 
TGCTTCTTTG GCCCCTCCCC CTCACAAGTG CTCTACCTCA TAGAGGTCGT GTATGCTTTG 
CTGATGCCAG CCAGTGCCAC TCTCGGTGAA GACGCCAGCG ACTTCCAATA CAACTTCCTG 
AAGAGCGGCG GCCTGCCCTT GGTGCTGAGC ATGCTCACCA GAAACAACTT TCTCCCATCG 
GCAGACATGG AAACTCGAAG GGGCGCCTAT GTCAACGCAC TAAAGATCGC AAAGCTCCTA 
CTGACCGCTG TGGGTTTTGG ACACGTGAAG GCGGTAGCTG AGGCCTGCCA GCCAAACGCC 
GACGGAAATA TTCCCGTCTC TCCAATTAAT CAGGCAACGC ACGACCAAGC GCTGGTTCTT 
CAGAGCGCCC TGCAAAGCAT CCCTAACCCG GCCTCAGAGT GCATGCTGCG TAATGTAGCC 
ATTCGCCTGG CCCAACAGAT CTCTGACGAG GTAACGCTTC TTGTNNNNNN NATTTGTGTA 
ATCCGAGCAA TACAGAAAAT CGTGTGGGCA TCAGGCTGTG GTACAGTGCA GCTCGTCTTT 
AGCTCCAATG AGGAGATCAG CAGGATATAT GAGAAGACAA ATGCTGCCAA GGAACCAGAT 
GGAGAGGATG AGCAGGTGTG CTGCGAGGCC TTGGAGGTCA TGACCCTGTG CTTTGCCCTC 
ATGCCCACGG CTCTGGACAC ACTAAGCAAA GAGAAGGCGT GGCAGACTTT TATCATAGAC 
CTGTTACTAC ACTGCCACAG CAAGTCCGTT CGACAAATGG CTCAGGAGCA GTTTTTCCTC 
ATGGCCACAA GATGCTGCAT GGGCCACCGA CCCCTCCTCT TCTTCATCAC CCTGTTATTC 
ACTGTGTTGG GGAGCACGGC CAAAGAGAGA GCCAAACATG CGGGGGACTA CTTCACCTTG 
CTCAGACACC TGCTAAACTA TGCTTACAAC AGCAACATCA ACCTGCCAAA CGCCGAGGTG 
CTGCTTAACA ATGAGATTGA CTGGCTCAAG AGAATACGGG ATGAGGTGAA GAGAACAGGA 
GAGACTGGTG TGGAGGAGAC GATCCTGGAG GGCCACCTTG GAGTCACCAA AGAGCTCCTT 
GCTTTTCAAA CACCGGAAAA GAAGTACTAC ATTGGCAGCG AGAAGGGAGG AGCCAACCTT 
ATAAAGGAGC TGATCGATGA CTTCATATTC CCAGCATCAA ATGTTTACCT GCAGTACATG 
AAGACGGGAG AGTTCCCCAC AGAGCAGGCC ATCCCAGTCT GCAGCACTCC CGCCTCCATC 
AATGCTGGCT TTGAGCTCCT GGTGGCTCTG GCCGTTGGCT GCATGCGCAA TCTGAAACAA 
ATAGTTGACA CCCTGACTGA TATGTACTAC TCAGGCGGGG AGACGTTGAC AGAGTGGGAG 
TACTTGCCTC CAGTTGGGCC ACGACCCAAT AAAGGCTTTG TAGGTCTAAA GAACGCCGGT 
GCCACATGTT ACATGAACTC TGTCATTCAG CAACTGTACA TGATCCCTCC CATTCGCAAC 
GGCATTCTGG CTATTGAGGG AACAGGCACG GATGTGGACG ATGACATGTC GGGGGATGAG 
AAGCAGGAGA ATGAGAGTAA CGTCGATCCT CGTGATGAGG TCTTCAGCTA CCATCACCAG 
TTTGATGACA AACCGTCCAG TAAGTCAGAG GACAGGAAGG AGTACAACAT CGGCGTGCTT 
CGTCACTTGC AGGTCATCTT TGGTCATCTT GCTGCATCCA GGCTGCAGTA CTACGTCCCA 
AGAGGCTTCT GGAAACAGTT TAGATTGTGG GGTGAGCCAG TCAACCTGAG GGAACAGCAC 
GATGCTCTGG AGTTTTTCAA CTCTTTGGTG GACAGTTTGG ATGAAGCTCT GAAGGCTCTG 
GGTCACCCCG CCATGCTCAG TAAGGTGCTG GGAGGCTCTT TCGCCGATCA GAAAATCTGT 
CAAGGGTGCC CTCACAGGTA TGAGTGTGAG GAGTCGTTTA CAACACTTAA CGTGGACATC 
AGAAACCACC AAAACCTGTT GGACTCCATG GAGCAGTATG TCAAAGGAGA TCTGCTGGAA 
GGAGCAAATG CCTATCACTG TGAGAAGTGT AATAAAAAGG TGGACACCGT GAAGCGTCTG 
TTGATCAAGA AGCTCCCACC CGTCCTGGCC ATTCAGCTGA AGCGCTTTGA CTACGACTGG 
GAGCGAGAAT GTGCCATCAA GTTTAACGAC TACTTTGAGT TCCCCAGGGA GCTGGATATG 
GAGCCTTACA CGGTAGCGGG CGTGGCCAAA CTGGAGGGCG ACGACGTGAA CCCGGAGAAC 
CAGGTCATAC AGCAGAACGA GCCGTCTGAA TCCACACCGC CGGGCAGCTC CAAATATCGT 
CTGGTGGGAG TGCTGGTCCA CTCAGGCCAG GCCAGTGGCG GACACTACTA TTCCTACATA 
ATCCAGAGGA ACGGAGGGGA CGGGGAGAAG AACCGCTGGT ATAAGTTCGA CGACGGCGAC 
GTCACTGAAT GCAAGATGGA CGACGAGGAG GAGATGAAGA ACCAGTGCTT CGGTGGGGAG 
TACATGGGCG AGGTGTTTGA TCATATGATG AAAAGGATGT CGTATCGGAG GCAAAAGCGC 
TGGTGGAACG CTTACATCCT GTTTTACGAG CGAATGGACT CGTTGGACAA GGACAGCGAT 
CTCGTCAAAT ACATCTCGGA CCTGACGATC TCCACCAAGC CACATCAGGT CAAGATGCCC 
ACGGTCATCG AGTGCAGCGT CCGCAAGCAG AACGTCCAGT TCATGCACAA CCGAATGCAA 
TACAGCCTAG AATATTTCCA GTTCATAAAG AAACTTCTGA CCTGTAACAG TGTCTATTTA 
AACCCTCCTC CAGGGCAAGA CCACCTGCTG CCAGAGGCAG AGGAGATCGC GATGATCAGC 
ACTCAGCTGG CTGCTAGGTT CCTCTTCAGC ACAGGTTTCC ACACCAAGAA AGTAGTACGG 
GGTCCTGCCA GTGACTGGTA TGACGCCCTC TGCATCCTGC TGAGACACAG TAAGAATGTT 
CGTTACTGGT TTGCACACAA CGTCCTGTTT GCGTACCCTA ACCGCTTCTC CGAGTATTTG 
CTGGAGTGCC CCAGCGCTGA GGTCCGCGGA GCATTTGCCA AGCTCATCGT CTTCATCGCT 
CACTTCTCCC TGCAAGATGG CCCCTGCCCC TCCCCCACCG CCTCACCTGG ACCGTCTGCT 
CAGGGCTGTG ATAATCTCAG TTTAAGTGAC CACTTGTTGA GAGCTGTTCT AAACCTGCTT 
AGGAGAGAGG TTTCTGAACA TGGCCGACAT CTACAGCAGT ACTTCAACCT CTTTGTCATG 
TATGCCAATC TGGGCCTGGC AGAGAAGTCT CAGCTGCTGA AGCTCAACGT TCCCGCCACT 
TTCATGCTGG TCGCTCTAGA CGAGGGTCCC GGTCCCCCCA TCAAATACCA GTATGCAGAA 
CTGGGCAAGC TCTACACTGT GGTTTCCCAG CTGGTCCGCT GCTGTGAGGT GTCCTCGCGC 
ATGCAATCCT CCATCAATGG TAACCCTCCT CTTCCTAACC CGTATGGGGA TCCCAACCTG 
ACGGCTCCGG TGATGTCTGT GCAGCAGCTG GTGGCAGAAA TCCTGTTTGT GAGGACGAGC 
TACGTCAAGA AGATCATCGA GGACTGCAGC AACTCTGAGG AGACCGTGAA GCTCCTTAGA 
TTCTGCTGCT GGGAGAATCC TCAGTTCTCC TCCACTGTGC TCAGTGAACT GCTTTGGCAG 
GTGGCATATT CTTACACGTA TGAGCTGAGG CCTTACCTGG ACTTGCTGCT ACAGATCCTT 
CTCATCGAAG ACTCCTGGCA GACGCACAGG ATCCACAATG TCCTAAAGGG CATCCCTGAT 
GACAGAGACG GGCTGTTTGA CACCATCCAG CGCTCCAAAA ACCATTACCA GAAGAGGGCC 
TACCAGTGCA TCAAGTGCAT GGTGGCCCTC TTTAGCAACT GCTCCGTGGC TTACCAGATC 
CTGCAGAGTA ATGGTGACCT GAAAAGGAAG TGGACGTGGG CGGTGGAGTG GTTGGGGGAT 
GAGTTGGAGA GAAGGCCGTA CACAGGAAAC CCGCAGTACA CCTACAACAA CTGGTCCCCC 
CCAGTTCAGA GCAACGAGAC CTCCAACGGG TATTTCCTGG AGCGTTCACA CAGTGCACGT 
ATGACACTTG CCAAAGCCTG TGAACTCTGT CCTGAAGAGG AGCCGGACGA ACAGGAAGCA 
CCTGATGATC AAGACTCCTC CCCTCCTGAA GACACCTCTC TGTACCCACA TTCTCCTGGA 
AGCACCCAGT TTCAGCAGGT ACTGCAGGAA AAACTGCATC ATAAAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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usp9x cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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