LOC101162908 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101162908
Entrez Gene ID	101162908
Full Name	nck-associated protein 5-like
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020708327.1	XP_020563986.1	nck-associated protein 5-like isoform X3
XM_020708323.1	XP_020563982.1	nck-associated protein 5-like isoform X1
XM_020708323.2	XP_020563982.1	nck-associated protein 5 isoform X1
XM_020708326.1	XP_020563985.1	nck-associated protein 5-like isoform X2
XM_020708329.1	XP_020563988.1	nck-associated protein 5-like isoform X4
XM_020708326.2	XP_020563985.1	nck-associated protein 5 isoform X2
XM_020708327.2	XP_020563986.1	nck-associated protein 5 isoform X3
XM_020708329.2	XP_020563988.1	nck-associated protein 5 isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC101162908 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101162908 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa27419	XM_020708326.2 Latest version!	Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa74623	XM_020708323.2 Latest version!	Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa74624	XM_020708327.2 Latest version!	Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa74625	XM_020708329.2 Latest version!	Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OJa74623	XM_020708323.1	Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa27419	XM_020708326.1	Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa74624	XM_020708327.1	Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa74625	XM_020708329.1	Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101162908 ( XM_020708326.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020563985.1 Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X2, mRNA.

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CloneID	OJa27419
Clone ID Related Accession (Same CDS sequence)	XM_020708326.1 , XM_020708326.2
Accession Version	XM_020708326.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	nck-associated protein 5-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGC ACCAGGAGAG GAGGGAACGT TTAAAAAGAT TGTCTCTGGA AAGCCGCCTG 
CCGGAGTACA TGGACGCCAA CAAATGCATC GATGAGCTTC TGAAACAGCT TGAGGAGGAG 
CGCCGGAATG TCCGAAGGGA AAAGCTGGCT GTGGCTCGCC TGCAGAGGGA AGTCGCACGA 
AGCAAGAGTG AAGGAACAAT GCGAGAAAAG TTGATCCACG AGCTAGAGGA AGAACGACGC 
CTGCGACTGG AGAGTGAGAA GCGTCTCCGG GAAGTGACGG AGGAGTCAGA GCTGGGAAGG 
GCGCAGATGA TTTCACTGCA GCAGCAGTTC TCCAGGATGG AAGAGACGGT GCGGTCGCTG 
CTGCAGAACC AGGGCGTCCT GGAGCAGACT GCTGTGGACA CAGTGGACAT CATGAAGGCC 
TACAAGGATA AACTCTCCGA GGAAGTGCAG AAACTGCAGG ATGGCCCAGA GGCGAAGCAG 
GCCGAGCCAG CGAGTACCGA GCTCGACGGC AGCCAAGCGG ACGAAGACAG AGACAAAACC 
AAGCTCCTTC TGGAGCGCCT GAAGACCCTC GAGGAGGAGA ACTCGGCGTT GGCCTCGGAG 
AACGAGAGCC AGAGGGAACA GTACGAGCGT TGCCTGGACG AGGTCGCTAA CCAAGTTGTA 
CAGGCACTCC TCACACAGAA GGATCTGAGA GAGGAATGTC TTAAGCTGCG GACAAGAGTT 
TTTGATCTGG AGCAGCAGAA TCGAGCACTG AGTGTTTTGT TCCAGCAGAA GATAAAGCCG 
GCATCAGACC TCCTCCTTCA GAAACTCCAC TCGCGGATCA TGGATCTGTC TGCGGCAGAT 
TTGCTTCTGG AGCCGGAGAG GAGCAAGGCC TTCCTCCTTT CCAGGAATAC AGACTCCCCT 
TCCAACGAGG TTCATTCGAA TGGAAAGCCA GGTGTCCCTG TGGCCAAATG TCTGAGCCAG 
ATGAGTCTGA TGGCGCCCGC TCCCATCTAT TCTCGCAGCA GCAGCGAGCT CTCACTGTCG 
AGCGCGTGCA GCGAGTTCTC CAGCGGCTCC TACACCTGGA ATGATGGGCG ATCCTGCGGG 
AAAATGTCAT CCCTGACGTG GGAGAAGAGA CTCAGTTTGG GCTCGTCAGC TCCAAGTAAC 
ATCTGCGCGC CGCTGGAGGA ACAGCCCCCC ACACGGCGTA AGGAGAGCCA CATACTGGAG 
GGCCTGAGAA AACTCCAGCG GAGGAAACAC AGGAGCTCCT CCTGCTCCTC TAGAGTGTCC 
AAGTCAGGCT ACAAAGATTG CATGAACTCC AACGAGGGCA TCTACTCTTT GGGTATCAAG 
AGCAGCAGCA AAGGGGTGTC TAAACCTGTA CATGTCAGCA GAGCTTCTGC TGCTCTTGGA 
AGGCGGTTTT CCTACGATTC CGATGATGCT GACGATGAAC TGGCACGCTC TAGTTGCAGA 
GATAATATTC CCACTAAGGA GAGCTGGATT CACTGCAAGA AGCTCTCCCA CAGCATCTCT 
GATAGTTTGT GCAGCTTGGA GGGGACACAG GAGGCTGTGG GTGATGGTAC TTCTTGTCCC 
ACAGCTTCAA AACCCTATGA CTCAAGGGAG CGTCCGGAGA AGCTCATGAG TTTCATCAAC 
AGTTTTCTTC CTTTAGAAGG GCGTAAATCA GCCTTTAGCA GGCCAACCGT GCACCACAGA 
AATCCTACTC AAGTAGAAGA TCCGAGTTGC AGATCCAACG CAGATGATCC AGAGGAAACC 
ATTCCTGATT CTGTGGGGGT CAAGACGCCA TGCAGTCAGC CGCAAGAACT AGCAGAGAGA 
GATATCAAGA GGCTCTCAAG GGATACCATC AAGATTTTCA AGCAACAGTT CATGCAAAAA 
GATCAGGGGC GCACTCAGTC TGCGGATGGG AGACCGAAGC CTTTCAGCCT ATTGAAGGAA 
CTTAAAGCAA CCAAGTGCAC CCAGTCTGAG GAGAGCATAC TGGCGATATT TGATGCTGAA 
GGAGAACCTA TTGATCTGTG CGCTCAGAGA CTAGTATCAG GAGCAGAAGG TCGCAAAGCC 
GCTAGCTGTT GCTCAGAGTT CCAAAAGAGG ACAACAAGGC AAAATTCAGC AAACACGACA 
AACTATAGCG TTCTTGAATC TCCAGAAAAA CCTTCAGAAC ATCAGACTAG ATCAGGCAAA 
GGCAGTGATA GCATGGAAGG AAGTGCAGAA AGGCTACAGT TAGCTCCTCA GAGGAAGTTG 
ATAAAGCCAC CAAGCAGTCG AACTAATAAA GGCTTTTCCA TTCCTCCCAT GAGTGACGTC 
AACAGCCCCA AATCTGGTGG CTCTAAGATC CCCGGTGTCA ATAAGCCCTT AGCTTCTCCG 
TTAAGAGTAT CCAAGGGCAC AACCACTGAG CCTAATAACA GTGGAAACTC AGGACCCTCT 
GCTCAGGAGA AATCTTCGTC TTCTCTTACG GTTAAGATGT CTAGATTCAT CAAGACGCAG 
GGAACTTCCA CTCAGAGTCC GAAAGCTGTG AACTGCAAGG TGTCTGGTCA GGCCGACTTA 
AGCAAGACCT CTTCTTCCAC TTCCCCCCAC CTTCCAAAAA GACGCCTCGA CTTCTCTGAC 
AACAGCGAAC AGCCGACCAG AGACAAACAC TGTGAAACCA GCAAGAACAA ACTCAGGTCT 
CCATCTCCTC CCCCTCCCCC AGGACGCACC ACCTCTTTAC TGGTCAGACC AAATTACGAA 
GCGTCGCCTC AAGCACACAA ATCAGGGGCT CAACCATCCA CGCCAACCAC TGTGAGGGGC 
CCTCCCCCAA GTTACCACAC CTCAGTTGCA CCAAATATGC AAGGCACGCT CCCCATCAAG 
GATAAAGACT GTTTAGACGT AGATGCAGGC TATGGGACTG CACTTGCACC TCAGAAGCTG 
GTGGAGAAAA CCACCCAGCA CTTTCAGAAG ACCACTGCCG TAACTCAGAC TCCAACTAAA 
GGCACTTCCA AGCGAATAAA AGACTACCTC CCACCTGCAA GCTCAGGATG TGCCAGAGTA 
CCTGAAATTG CACCTAAAAA CTCGAAGAAT GTCCCTCCTC CCTACAGTGC TCTAAGAGTG 
CCCTCCCTTC AAAACTCCTT TGCAAACAAA AAGGTACCTG CTCAAGAAAA CACCCATCAG 
TCAGTGCAAA AGACTTCCAT TAGTTTACCA ATATCGCTTC AGGATTCTGC TCAGTGTCAA 
ACAGAGCCAC AAAAAGCTGT CATGAGCCCT CCGGGCTCAG CTGTTACGTC ACCCAATTTG 
TCAGAAAAAG CCTCAAAGAC TCGAATCCCA ATGGGATTTA AAGCCTTTCT AAAATCCCCT 
CCCAGCCATA AAAGTAGTCC TACAATAGCA GGCAAGCAAG AGAAAGATCA TATCAACTCT 
GTCTCCAAGG AAACTGTGAC TTCAAATGCT TCTACTCAGT GTGACACCTC GCAGGCAGCG 
TACAGTACGG ATTCAACACC TACCGTGTCT GTTCCCGAAG GGAAAGGTGA ATTTCTGTGT 
CAGCCACAGA AAGAGGACTT ACAACCCCCC TTGTCGTCAG CAGAAGGAAA TGTGAGTGAC 
AAGGCAAAAA GGAGTAGTCA ACTCTTTGGC AGATCCATAT CTATTAATAC CAAACCACAC 
CTAAAGCCAG CTTTAGGGAT GAACGGGGCC AAAGCTCGAA GCCAGAGCTT CAGCACCAAC 
TACATAGAGA AACCCAACAT CAATGCCATG GAGGGGCCGG GAAGGATCAG GACTCAAATC 
ATCACTAACT CAGGTGAAAG GGGGAATTCG CTGTCAAGGC AGAGTTCTTC AGAAACAACT 
GGGGTTGGAT TGGCTGAAAG TCCAATCCAT GCCCCTAGAA CCCGGCTAAG CCATCATGGT 
GGCATGATGG GACCAAATAG TCAGAGCGGC CTCCCTGAGA AGTCCATTCA GTCGTGTGTT 
ATAGGTGAAG GAAAAGCTTG TGGAGTGAAA GGAGATGTGG TCACCTCTCC TTCTCAAAGA 
GAAGCTCGCA GCTTGCCCAA AAGTGACAGA ACTGCCTTTA TTAATGTCCA AAAGCCGGCA 
AAAGTGGACT CCCATCCTCA AATCCAGTCT TCCTCAGCTT TTGTCTTTAG TCCTGAAAAT 
CCTACCAGAG AAGCAGAAAC CATTACCACC ACTCCTGCTG AGCCAGACAA AACCCAGTTG 
GGTTCCCCAG GCAATTTCCC AGACACAGAG GAGAAAAAAA TCAGCCCAAC AACGTGCACT 
ATTGAAGAGA AAGTGATGAT GGGGATAGAA GAAAACCTGC AGAAAAGTCA AGAGCAGGAG 
AAGGCTGCAG CTAATGAGGC CAAACAGAAG ACGGGCCCGT CTTTGGCCAA CTGGTTTGGC 
CTCCGCAAGA GCAAACTACC AGCTTTTAGC GGCAAAAAAG CTGATTCCCC GAAGGCAAGA 
GACGAGAAGA AAGAGCTGAA GATTGGGTCT GTGATCAGCG GCAAGCAGAT GAAGTCTGAT 
AAGAAGAAGG AAAGGAAGAA GGATGGTCAG GAGGTCCAGA ATTTGTCAGA GATGAACAAC 
AAGCTGAGCT CCATCATGGA CCACTGTAAC AATCAGATGG GTCAGATCGC CAGTCAGATC 
CAGAGCACCA CTGTGTTAAT CGGCAAGGAC CAGTTTGTAA AAGAGCTTCT TGGGAGAGCT 
ACTGTGAAGG GCAACGCTTC GCCACCTGGC ATCTCTACAC CGAAGAAACA CTGTGAAACA 
AAGGGCGACA TGCAGATCTG TCCAGATAAT ACCCTCATAC TGACGCAAAA GATCAGCCTC 
AAGGCCAAAA ACGACGAGGG GAACATTCCG GACACGTCTT GTCAAGACCA CATGATAGGC 
TCCAGCTGTC AGATGAGAAC CTTGGACAGC GGCATTGGAA CCTTCCCCCT GCCCGATTCG 
GTCACGCGGG CCAGCAGTCG CCACATCCCC AAATCTGAGT CCAGTCCAGA TGGGGTGATG 
GCCGGCTCAA CTGAGCGCAG TCGGGACACT CCCTCCTCCC ACCCTGACCC CTCACAGTCA 
GATTTCAAAG TGCCTTCCCC TTCAAAAGCC CATGTGCACA CCCCCACTAC CAGCATGGGT 
CACTCCCTGT CTGATCCCAC TGTGACATGC AGCAGGAATG CCCTGGAGAC CCAGAGTCGC 
CTGCCCAAGC TGGCATCCAC AGACGCAATC AAGACCAAAA GGTTGAGTCT TTGTGCCCAG 
CGCAGCGCCG TCTCCGCTTC TGCCGAGGGA GACGGGAAAG AAATGGAGAG GAAGATGAAA 
ACAAGCGAGC ATGAATCACC TGGTGAGACA ACATTGCGGG TTTGCGTGTA TCCAGGCAGC 
GACAGTGACA CGGAGACTGA ATCCGAGGGA ACTACAAGCA CTTTGGCGTC GCCGCTAAGA 
ACCCCCATGG ACAGACCTCA GAAGAACGAC TCTGTCGAGC AGAATGAGGA GACCTTGAAA 
AAACAGAATG CAGAGAAATC CCTGTCCATT ATGGACTACT ATCAGCACGA TGTGTTCTTG 
CACCTGGAGA CAGACGCCAG GAGGATTTCC CAGTACACCT TGCTGCACAA AGAGTCGTCC 
CTCGACGGAG AAGCAGGAGA CAGGCTCAGC AAAGAGATTC CTGTCAGTCC CAACCAGCCG 
GCCTCTTTGG ACGTCTCCTT GGAATCGTTG AACAAAATGA GCCACAGCAG CTCCAGCTGC 
CTGTGCGCGG ACGCCAGCCA CCGAGCCGGC TGCCCCGTAG AGACGGGGAG GACCGCAGAG 
AGCTGCTGCA AGGTCAGCCA GTCCTCCTGC TCCTCCAGCT TCATCAGCAG GCCCGGGGTG 
GACCCCGCGG GCTCCCTTAG CGACTCCCTC TACGACAGTT TCTCCTCCTG CACCAGTCAG 
GGCTCCAACG ATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020563985.1
CDS	442..6279
Translation	MDRHQERRERLKRLSLESRLPEYMDANKCIDELLKQLEEERRNVRREKLAVARLQREVARSKSEGTMREKLIHELEEERRLRLESEKRLREVTEESELGRAQMISLQQQFSRMEETVRSLLQNQGVLEQTAVDTVDIMKAYKDKLSEEVQKLQDGPEAKQAEPASTELDGSQADEDRDKTKLLLERLKTLEEENSALASENESQREQYERCLDEVANQVVQALLTQKDLREECLKLRTRVFDLEQQNRALSVLFQQKIKPASDLLLQKLHSRIMDLSAADLLLEPERSKAFLLSRNTDSPSNEVHSNGKPGVPVAKCLSQMSLMAPAPIYSRSSSELSLSSACSEFSSGSYTWNDGRSCGKMSSLTWEKRLSLGSSAPSNICAPLEEQPPTRRKESHILEGLRKLQRRKHRSSSCSSRVSKSGYKDCMNSNEGIYSLGIKSSSKGVSKPVHVSRASAALGRRFSYDSDDADDELARSSCRDNIPTKESWIHCKKLSHSISDSLCSLEGTQEAVGDGTSCPTASKPYDSRERPEKLMSFINSFLPLEGRKSAFSRPTVHHRNPTQVEDPSCRSNADDPEETIPDSVGVKTPCSQPQELAERDIKRLSRDTIKIFKQQFMQKDQGRTQSADGRPKPFSLLKELKATKCTQSEESILAIFDAEGEPIDLCAQRLVSGAEGRKAASCCSEFQKRTTRQNSANTTNYSVLESPEKPSEHQTRSGKGSDSMEGSAERLQLAPQRKLIKPPSSRTNKGFSIPPMSDVNSPKSGGSKIPGVNKPLASPLRVSKGTTTEPNNSGNSGPSAQEKSSSSLTVKMSRFIKTQGTSTQSPKAVNCKVSGQADLSKTSSSTSPHLPKRRLDFSDNSEQPTRDKHCETSKNKLRSPSPPPPPGRTTSLLVRPNYEASPQAHKSGAQPSTPTTVRGPPPSYHTSVAPNMQGTLPIKDKDCLDVDAGYGTALAPQKLVEKTTQHFQKTTAVTQTPTKGTSKRIKDYLPPASSGCARVPEIAPKNSKNVPPPYSALRVPSLQNSFANKKVPAQENTHQSVQKTSISLPISLQDSAQCQTEPQKAVMSPPGSAVTSPNLSEKASKTRIPMGFKAFLKSPPSHKSSPTIAGKQEKDHINSVSKETVTSNASTQCDTSQAAYSTDSTPTVSVPEGKGEFLCQPQKEDLQPPLSSAEGNVSDKAKRSSQLFGRSISINTKPHLKPALGMNGAKARSQSFSTNYIEKPNINAMEGPGRIRTQIITNSGERGNSLSRQSSSETTGVGLAESPIHAPRTRLSHHGGMMGPNSQSGLPEKSIQSCVIGEGKACGVKGDVVTSPSQREARSLPKSDRTAFINVQKPAKVDSHPQIQSSSAFVFSPENPTREAETITTTPAEPDKTQLGSPGNFPDTEEKKISPTTCTIEEKVMMGIEENLQKSQEQEKAAANEAKQKTGPSLANWFGLRKSKLPAFSGKKADSPKARDEKKELKIGSVISGKQMKSDKKKERKKDGQEVQNLSEMNNKLSSIMDHCNNQMGQIASQIQSTTVLIGKDQFVKELLGRATVKGNASPPGISTPKKHCETKGDMQICPDNTLILTQKISLKAKNDEGNIPDTSCQDHMIGSSCQMRTLDSGIGTFPLPDSVTRASSRHIPKSESSPDGVMAGSTERSRDTPSSHPDPSQSDFKVPSPSKAHVHTPTTSMGHSLSDPTVTCSRNALETQSRLPKLASTDAIKTKRLSLCAQRSAVSASAEGDGKEMERKMKTSEHESPGETTLRVCVYPGSDSDTETESEGTTSTLASPLRTPMDRPQKNDSVEQNEETLKKQNAEKSLSIMDYYQHDVFLHLETDARRISQYTLLHKESSLDGEAGDRLSKEIPVSPNQPASLDVSLESLNKMSHSSSSCLCADASHRAGCPVETGRTAESCCKVSQSSCSSSFISRPGVDPAGSLSDSLYDSFSSCTSQGSNDV

Target ORF information:

RefSeq Version	XM_020708326.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020708326.1

ORF Insert Sequence:

ATGGACAGGC ACCAGGAGAG GAGGGAACGT TTAAAAAGAT TGTCTCTGGA AAGCCGCCTG 
CCGGAGTACA TGGACGCCAA CAAATGCATC GATGAGCTTC TGAAACAGCT TGAGGAGGAG 
CGCCGGAATG TCCGAAGGGA AAAGCTGGCT GTGGCTCGCC TGCAGAGGGA AGTCGCACGA 
AGCAAGAGTG AAGGAACAAT GCGAGAAAAG TTGATCCACG AGCTAGAGGA AGAACGACGC 
CTGCGACTGG AGAGTGAGAA GCGTCTCCGG GAAGTGACGG AGGAGTCAGA GCTGGGAAGG 
GCGCAGATGA TTTCACTGCA GCAGCAGTTC TCCAGGATGG AAGAGACGGT GCGGTCGCTG 
CTGCAGAACC AGGGCGTCCT GGAGCAGACT GCTGTGGACA CAGTGGACAT CATGAAGGCC 
TACAAGGATA AACTCTCCGA GGAAGTGCAG AAACTGCAGG ATGGCCCAGA GGCGAAGCAG 
GCCGAGCCAG CGAGTACCGA GCTCGACGGC AGCCAAGCGG ACGAAGACAG AGACAAAACC 
AAGCTCCTTC TGGAGCGCCT GAAGACCCTC GAGGAGGAGA ACTCGGCGTT GGCCTCGGAG 
AACGAGAGCC AGAGGGAACA GTACGAGCGT TGCCTGGACG AGGTCGCTAA CCAAGTTGTA 
CAGGCACTCC TCACACAGAA GGATCTGAGA GAGGAATGTC TTAAGCTGCG GACAAGAGTT 
TTTGATCTGG AGCAGCAGAA TCGAGCACTG AGTGTTTTGT TCCAGCAGAA GATAAAGCCG 
GCATCAGACC TCCTCCTTCA GAAACTCCAC TCGCGGATCA TGGATCTGTC TGCGGCAGAT 
TTGCTTCTGG AGCCGGAGAG GAGCAAGGCC TTCCTCCTTT CCAGGAATAC AGACTCCCCT 
TCCAACGAGG TTCATTCGAA TGGAAAGCCA GGTGTCCCTG TGGCCAAATG TCTGAGCCAG 
ATGAGTCTGA TGGCGCCCGC TCCCATCTAT TCTCGCAGCA GCAGCGAGCT CTCACTGTCG 
AGCGCGTGCA GCGAGTTCTC CAGCGGCTCC TACACCTGGA ATGATGGGCG ATCCTGCGGG 
AAAATGTCAT CCCTGACGTG GGAGAAGAGA CTCAGTTTGG GCTCGTCAGC TCCAAGTAAC 
ATCTGCGCGC CGCTGGAGGA ACAGCCCCCC ACACGGCGTA AGGAGAGCCA CATACTGGAG 
GGCCTGAGAA AACTCCAGCG GAGGAAACAC AGGAGCTCCT CCTGCTCCTC TAGAGTGTCC 
AAGTCAGGCT ACAAAGATTG CATGAACTCC AACGAGGGCA TCTACTCTTT GGGTATCAAG 
AGCAGCAGCA AAGGGGTGTC TAAACCTGTA CATGTCAGCA GAGCTTCTGC TGCTCTTGGA 
AGGCGGTTTT CCTACGATTC CGATGATGCT GACGATGAAC TGGCACGCTC TAGTTGCAGA 
GATAATATTC CCACTAAGGA GAGCTGGATT CACTGCAAGA AGCTCTCCCA CAGCATCTCT 
GATAGTTTGT GCAGCTTGGA GGGGACACAG GAGGCTGTGG GTGATGGTAC TTCTTGTCCC 
ACAGCTTCAA AACCCTATGA CTCAAGGGAG CGTCCGGAGA AGCTCATGAG TTTCATCAAC 
AGTTTTCTTC CTTTAGAAGG GCGTAAATCA GCCTTTAGCA GGCCAACCGT GCACCACAGA 
AATCCTACTC AAGTAGAAGA TCCGAGTTGC AGATCCAACG CAGATGATCC AGAGGAAACC 
ATTCCTGATT CTGTGGGGGT CAAGACGCCA TGCAGTCAGC CGCAAGAACT AGCAGAGAGA 
GATATCAAGA GGCTCTCAAG GGATACCATC AAGATTTTCA AGCAACAGTT CATGCAAAAA 
GATCAGGGGC GCACTCAGTC TGCGGATGGG AGACCGAAGC CTTTCAGCCT ATTGAAGGAA 
CTTAAAGCAA CCAAGTGCAC CCAGTCTGAG GAGAGCATAC TGGCGATATT TGATGCTGAA 
GGAGAACCTA TTGATCTGTG CGCTCAGAGA CTAGTATCAG GAGCAGAAGG TCGCAAAGCC 
GCTAGCTGTT GCTCAGAGTT CCAAAAGAGG ACAACAAGGC AAAATTCAGC AAACACGACA 
AACTATAGCG TTCTTGAATC TCCAGAAAAA CCTTCAGAAC ATCAGACTAG ATCAGGCAAA 
GGCAGTGATA GCATGGAAGG AAGTGCAGAA AGGCTACAGT TAGCTCCTCA GAGGAAGTTG 
ATAAAGCCAC CAAGCAGTCG AACTAATAAA GGCTTTTCCA TTCCTCCCAT GAGTGACGTC 
AACAGCCCCA AATCTGGTGG CTCTAAGATC CCCGGTGTCA ATAAGCCCTT AGCTTCTCCG 
TTAAGAGTAT CCAAGGGCAC AACCACTGAG CCTAATAACA GTGGAAACTC AGGACCCTCT 
GCTCAGGAGA AATCTTCGTC TTCTCTTACG GTTAAGATGT CTAGATTCAT CAAGACGCAG 
GGAACTTCCA CTCAGAGTCC GAAAGCTGTG AACTGCAAGG TGTCTGGTCA GGCCGACTTA 
AGCAAGACCT CTTCTTCCAC TTCCCCCCAC CTTCCAAAAA GACGCCTCGA CTTCTCTGAC 
AACAGCGAAC AGCCGACCAG AGACAAACAC TGTGAAACCA GCAAGAACAA ACTCAGGTCT 
CCATCTCCTC CCCCTCCCCC AGGACGCACC ACCTCTTTAC TGGTCAGACC AAATTACGAA 
GCGTCGCCTC AAGCACACAA ATCAGGGGCT CAACCATCCA CGCCAACCAC TGTGAGGGGC 
CCTCCCCCAA GTTACCACAC CTCAGTTGCA CCAAATATGC AAGGCACGCT CCCCATCAAG 
GATAAAGACT GTTTAGACGT AGATGCAGGC TATGGGACTG CACTTGCACC TCAGAAGCTG 
GTGGAGAAAA CCACCCAGCA CTTTCAGAAG ACCACTGCCG TAACTCAGAC TCCAACTAAA 
GGCACTTCCA AGCGAATAAA AGACTACCTC CCACCTGCAA GCTCAGGATG TGCCAGAGTA 
CCTGAAATTG CACCTAAAAA CTCGAAGAAT GTCCCTCCTC CCTACAGTGC TCTAAGAGTG 
CCCTCCCTTC AAAACTCCTT TGCAAACAAA AAGGTACCTG CTCAAGAAAA CACCCATCAG 
TCAGTGCAAA AGACTTCCAT TAGTTTACCA ATATCGCTTC AGGATTCTGC TCAGTGTCAA 
ACAGAGCCAC AAAAAGCTGT CATGAGCCCT CCGGGCTCAG CTGTTACGTC ACCCAATTTG 
TCAGAAAAAG CCTCAAAGAC TCGAATCCCA ATGGGATTTA AAGCCTTTCT AAAATCCCCT 
CCCAGCCATA AAAGTAGTCC TACAATAGCA GGCAAGCAAG AGAAAGATCA TATCAACTCT 
GTCTCCAAGG AAACTGTGAC TTCAAATGCT TCTACTCAGT GTGACACCTC GCAGGCAGCG 
TACAGTACGG ATTCAACACC TACCGTGTCT GTTCCCGAAG GGAAAGGTGA ATTTCTGTGT 
CAGCCACAGA AAGAGGACTT ACAACCCCCC TTGTCGTCAG CAGAAGGAAA TGTGAGTGAC 
AAGGCAAAAA GGAGTAGTCA ACTCTTTGGC AGATCCATAT CTATTAATAC CAAACCACAC 
CTAAAGCCAG CTTTAGGGAT GAACGGGGCC AAAGCTCGAA GCCAGAGCTT CAGCACCAAC 
TACATAGAGA AACCCAACAT CAATGCCATG GAGGGGCCGG GAAGGATCAG GACTCAAATC 
ATCACTAACT CAGGTGAAAG GGGGAATTCG CTGTCAAGGC AGAGTTCTTC AGAAACAACT 
GGGGTTGGAT TGGCTGAAAG TCCAATCCAT GCCCCTAGAA CCCGGCTAAG CCATCATGGT 
GGCATGATGG GACCAAATAG TCAGAGCGGC CTCCCTGAGA AGTCCATTCA GTCGTGTGTT 
ATAGGTGAAG GAAAAGCTTG TGGAGTGAAA GGAGATGTGG TCACCTCTCC TTCTCAAAGA 
GAAGCTCGCA GCTTGCCCAA AAGTGACAGA ACTGCCTTTA TTAATGTCCA AAAGCCGGCA 
AAAGTGGACT CCCATCCTCA AATCCAGTCT TCCTCAGCTT TTGTCTTTAG TCCTGAAAAT 
CCTACCAGAG AAGCAGAAAC CATTACCACC ACTCCTGCTG AGCCAGACAA AACCCAGTTG 
GGTTCCCCAG GCAATTTCCC AGACACAGAG GAGAAAAAAA TCAGCCCAAC AACGTGCACT 
ATTGAAGAGA AAGTGATGAT GGGGATAGAA GAAAACCTGC AGAAAAGTCA AGAGCAGGAG 
AAGGCTGCAG CTAATGAGGC CAAACAGAAG ACGGGCCCGT CTTTGGCCAA CTGGTTTGGC 
CTCCGCAAGA GCAAACTACC AGCTTTTAGC GGCAAAAAAG CTGATTCCCC GAAGGCAAGA 
GACGAGAAGA AAGAGCTGAA GATTGGGTCT GTGATCAGCG GCAAGCAGAT GAAGTCTGAT 
AAGAAGAAGG AAAGGAAGAA GGATGGTCAG GAGGTCCAGA ATTTGTCAGA GATGAACAAC 
AAGCTGAGCT CCATCATGGA CCACTGTAAC AATCAGATGG GTCAGATCGC CAGTCAGATC 
CAGAGCACCA CTGTGTTAAT CGGCAAGGAC CAGTTTGTAA AAGAGCTTCT TGGGAGAGCT 
ACTGTGAAGG GCAACGCTTC GCCACCTGGC ATCTCTACAC CGAAGAAACA CTGTGAAACA 
AAGGGCGACA TGCAGATCTG TCCAGATAAT ACCCTCATAC TGACGCAAAA GATCAGCCTC 
AAGGCCAAAA ACGACGAGGG GAACATTCCG GACACGTCTT GTCAAGACCA CATGATAGGC 
TCCAGCTGTC AGATGAGAAC CTTGGACAGC GGCATTGGAA CCTTCCCCCT GCCCGATTCG 
GTCACGCGGG CCAGCAGTCG CCACATCCCC AAATCTGAGT CCAGTCCAGA TGGGGTGATG 
GCCGGCTCAA CTGAGCGCAG TCGGGACACT CCCTCCTCCC ACCCTGACCC CTCACAGTCA 
GATTTCAAAG TGCCTTCCCC TTCAAAAGCC CATGTGCACA CCCCCACTAC CAGCATGGGT 
CACTCCCTGT CTGATCCCAC TGTGACATGC AGCAGGAATG CCCTGGAGAC CCAGAGTCGC 
CTGCCCAAGC TGGCATCCAC AGACGCAATC AAGACCAAAA GGTTGAGTCT TTGTGCCCAG 
CGCAGCGCCG TCTCCGCTTC TGCCGAGGGA GACGGGAAAG AAATGGAGAG GAAGATGAAA 
ACAAGCGAGC ATGAATCACC TGGTGAGACA ACATTGCGGG TTTGCGTGTA TCCAGGCAGC 
GACAGTGACA CGGAGACTGA ATCCGAGGGA ACTACAAGCA CTTTGGCGTC GCCGCTAAGA 
ACCCCCATGG ACAGACCTCA GAAGAACGAC TCTGTCGAGC AGAATGAGGA GACCTTGAAA 
AAACAGAATG CAGAGAAATC CCTGTCCATT ATGGACTACT ATCAGCACGA TGTGTTCTTG 
CACCTGGAGA CAGACGCCAG GAGGATTTCC CAGTACACCT TGCTGCACAA AGAGTCGTCC 
CTCGACGGAG AAGCAGGAGA CAGGCTCAGC AAAGAGATTC CTGTCAGTCC CAACCAGCCG 
GCCTCTTTGG ACGTCTCCTT GGAATCGTTG AACAAAATGA GCCACAGCAG CTCCAGCTGC 
CTGTGCGCGG ACGCCAGCCA CCGAGCCGGC TGCCCCGTAG AGACGGGGAG GACCGCAGAG 
AGCTGCTGCA AGGTCAGCCA GTCCTCCTGC TCCTCCAGCT TCATCAGCAG GCCCGGGGTG 
GACCCCGCGG GCTCCCTTAG CGACTCCCTC TACGACAGTT TCTCCTCCTG CACCAGTCAG 
GGCTCCAACG ATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101162908 ( XM_020708326.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020563985.1 Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X2, mRNA.

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CloneID	OJa27419
Clone ID Related Accession (Same CDS sequence)	XM_020708326.1 , XM_020708326.2
Accession Version	XM_020708326.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	nck-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020708326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGC ACCAGGAGAG GAGGGAACGT TTAAAAAGAT TGTCTCTGGA AAGCCGCCTG 
CCGGAGTACA TGGACGCCAA CAAATGCATC GATGAGCTTC TGAAACAGCT TGAGGAGGAG 
CGCCGGAATG TCCGAAGGGA AAAGCTGGCT GTGGCTCGCC TGCAGAGGGA AGTCGCACGA 
AGCAAGAGTG AAGGAACAAT GCGAGAAAAG TTGATCCACG AGCTAGAGGA AGAACGACGC 
CTGCGACTGG AGAGTGAGAA GCGTCTCCGG GAAGTGACGG AGGAGTCAGA GCTGGGAAGG 
GCGCAGATGA TTTCACTGCA GCAGCAGTTC TCCAGGATGG AAGAGACGGT GCGGTCGCTG 
CTGCAGAACC AGGGCGTCCT GGAGCAGACT GCTGTGGACA CAGTGGACAT CATGAAGGCC 
TACAAGGATA AACTCTCCGA GGAAGTGCAG AAACTGCAGG ATGGCCCAGA GGCGAAGCAG 
GCCGAGCCAG CGAGTACCGA GCTCGACGGC AGCCAAGCGG ACGAAGACAG AGACAAAACC 
AAGCTCCTTC TGGAGCGCCT GAAGACCCTC GAGGAGGAGA ACTCGGCGTT GGCCTCGGAG 
AACGAGAGCC AGAGGGAACA GTACGAGCGT TGCCTGGACG AGGTCGCTAA CCAAGTTGTA 
CAGGCACTCC TCACACAGAA GGATCTGAGA GAGGAATGTC TTAAGCTGCG GACAAGAGTT 
TTTGATCTGG AGCAGCAGAA TCGAGCACTG AGTGTTTTGT TCCAGCAGAA GATAAAGCCG 
GCATCAGACC TCCTCCTTCA GAAACTCCAC TCGCGGATCA TGGATCTGTC TGCGGCAGAT 
TTGCTTCTGG AGCCGGAGAG GAGCAAGGCC TTCCTCCTTT CCAGGAATAC AGACTCCCCT 
TCCAACGAGG TTCATTCGAA TGGAAAGCCA GGTGTCCCTG TGGCCAAATG TCTGAGCCAG 
ATGAGTCTGA TGGCGCCCGC TCCCATCTAT TCTCGCAGCA GCAGCGAGCT CTCACTGTCG 
AGCGCGTGCA GCGAGTTCTC CAGCGGCTCC TACACCTGGA ATGATGGGCG ATCCTGCGGG 
AAAATGTCAT CCCTGACGTG GGAGAAGAGA CTCAGTTTGG GCTCGTCAGC TCCAAGTAAC 
ATCTGCGCGC CGCTGGAGGA ACAGCCCCCC ACACGGCGTA AGGAGAGCCA CATACTGGAG 
GGCCTGAGAA AACTCCAGCG GAGGAAACAC AGGAGCTCCT CCTGCTCCTC TAGAGTGTCC 
AAGTCAGGCT ACAAAGATTG CATGAACTCC AACGAGGGCA TCTACTCTTT GGGTATCAAG 
AGCAGCAGCA AAGGGGTGTC TAAACCTGTA CATGTCAGCA GAGCTTCTGC TGCTCTTGGA 
AGGCGGTTTT CCTACGATTC CGATGATGCT GACGATGAAC TGGCACGCTC TAGTTGCAGA 
GATAATATTC CCACTAAGGA GAGCTGGATT CACTGCAAGA AGCTCTCCCA CAGCATCTCT 
GATAGTTTGT GCAGCTTGGA GGGGACACAG GAGGCTGTGG GTGATGGTAC TTCTTGTCCC 
ACAGCTTCAA AACCCTATGA CTCAAGGGAG CGTCCGGAGA AGCTCATGAG TTTCATCAAC 
AGTTTTCTTC CTTTAGAAGG GCGTAAATCA GCCTTTAGCA GGCCAACCGT GCACCACAGA 
AATCCTACTC AAGTAGAAGA TCCGAGTTGC AGATCCAACG CAGATGATCC AGAGGAAACC 
ATTCCTGATT CTGTGGGGGT CAAGACGCCA TGCAGTCAGC CGCAAGAACT AGCAGAGAGA 
GATATCAAGA GGCTCTCAAG GGATACCATC AAGATTTTCA AGCAACAGTT CATGCAAAAA 
GATCAGGGGC GCACTCAGTC TGCGGATGGG AGACCGAAGC CTTTCAGCCT ATTGAAGGAA 
CTTAAAGCAA CCAAGTGCAC CCAGTCTGAG GAGAGCATAC TGGCGATATT TGATGCTGAA 
GGAGAACCTA TTGATCTGTG CGCTCAGAGA CTAGTATCAG GAGCAGAAGG TCGCAAAGCC 
GCTAGCTGTT GCTCAGAGTT CCAAAAGAGG ACAACAAGGC AAAATTCAGC AAACACGACA 
AACTATAGCG TTCTTGAATC TCCAGAAAAA CCTTCAGAAC ATCAGACTAG ATCAGGCAAA 
GGCAGTGATA GCATGGAAGG AAGTGCAGAA AGGCTACAGT TAGCTCCTCA GAGGAAGTTG 
ATAAAGCCAC CAAGCAGTCG AACTAATAAA GGCTTTTCCA TTCCTCCCAT GAGTGACGTC 
AACAGCCCCA AATCTGGTGG CTCTAAGATC CCCGGTGTCA ATAAGCCCTT AGCTTCTCCG 
TTAAGAGTAT CCAAGGGCAC AACCACTGAG CCTAATAACA GTGGAAACTC AGGACCCTCT 
GCTCAGGAGA AATCTTCGTC TTCTCTTACG GTTAAGATGT CTAGATTCAT CAAGACGCAG 
GGAACTTCCA CTCAGAGTCC GAAAGCTGTG AACTGCAAGG TGTCTGGTCA GGCCGACTTA 
AGCAAGACCT CTTCTTCCAC TTCCCCCCAC CTTCCAAAAA GACGCCTCGA CTTCTCTGAC 
AACAGCGAAC AGCCGACCAG AGACAAACAC TGTGAAACCA GCAAGAACAA ACTCAGGTCT 
CCATCTCCTC CCCCTCCCCC AGGACGCACC ACCTCTTTAC TGGTCAGACC AAATTACGAA 
GCGTCGCCTC AAGCACACAA ATCAGGGGCT CAACCATCCA CGCCAACCAC TGTGAGGGGC 
CCTCCCCCAA GTTACCACAC CTCAGTTGCA CCAAATATGC AAGGCACGCT CCCCATCAAG 
GATAAAGACT GTTTAGACGT AGATGCAGGC TATGGGACTG CACTTGCACC TCAGAAGCTG 
GTGGAGAAAA CCACCCAGCA CTTTCAGAAG ACCACTGCCG TAACTCAGAC TCCAACTAAA 
GGCACTTCCA AGCGAATAAA AGACTACCTC CCACCTGCAA GCTCAGGATG TGCCAGAGTA 
CCTGAAATTG CACCTAAAAA CTCGAAGAAT GTCCCTCCTC CCTACAGTGC TCTAAGAGTG 
CCCTCCCTTC AAAACTCCTT TGCAAACAAA AAGGTACCTG CTCAAGAAAA CACCCATCAG 
TCAGTGCAAA AGACTTCCAT TAGTTTACCA ATATCGCTTC AGGATTCTGC TCAGTGTCAA 
ACAGAGCCAC AAAAAGCTGT CATGAGCCCT CCGGGCTCAG CTGTTACGTC ACCCAATTTG 
TCAGAAAAAG CCTCAAAGAC TCGAATCCCA ATGGGATTTA AAGCCTTTCT AAAATCCCCT 
CCCAGCCATA AAAGTAGTCC TACAATAGCA GGCAAGCAAG AGAAAGATCA TATCAACTCT 
GTCTCCAAGG AAACTGTGAC TTCAAATGCT TCTACTCAGT GTGACACCTC GCAGGCAGCG 
TACAGTACGG ATTCAACACC TACCGTGTCT GTTCCCGAAG GGAAAGGTGA ATTTCTGTGT 
CAGCCACAGA AAGAGGACTT ACAACCCCCC TTGTCGTCAG CAGAAGGAAA TGTGAGTGAC 
AAGGCAAAAA GGAGTAGTCA ACTCTTTGGC AGATCCATAT CTATTAATAC CAAACCACAC 
CTAAAGCCAG CTTTAGGGAT GAACGGGGCC AAAGCTCGAA GCCAGAGCTT CAGCACCAAC 
TACATAGAGA AACCCAACAT CAATGCCATG GAGGGGCCGG GAAGGATCAG GACTCAAATC 
ATCACTAACT CAGGTGAAAG GGGGAATTCG CTGTCAAGGC AGAGTTCTTC AGAAACAACT 
GGGGTTGGAT TGGCTGAAAG TCCAATCCAT GCCCCTAGAA CCCGGCTAAG CCATCATGGT 
GGCATGATGG GACCAAATAG TCAGAGCGGC CTCCCTGAGA AGTCCATTCA GTCGTGTGTT 
ATAGGTGAAG GAAAAGCTTG TGGAGTGAAA GGAGATGTGG TCACCTCTCC TTCTCAAAGA 
GAAGCTCGCA GCTTGCCCAA AAGTGACAGA ACTGCCTTTA TTAATGTCCA AAAGCCGGCA 
AAAGTGGACT CCCATCCTCA AATCCAGTCT TCCTCAGCTT TTGTCTTTAG TCCTGAAAAT 
CCTACCAGAG AAGCAGAAAC CATTACCACC ACTCCTGCTG AGCCAGACAA AACCCAGTTG 
GGTTCCCCAG GCAATTTCCC AGACACAGAG GAGAAAAAAA TCAGCCCAAC AACGTGCACT 
ATTGAAGAGA AAGTGATGAT GGGGATAGAA GAAAACCTGC AGAAAAGTCA AGAGCAGGAG 
AAGGCTGCAG CTAATGAGGC CAAACAGAAG ACGGGCCCGT CTTTGGCCAA CTGGTTTGGC 
CTCCGCAAGA GCAAACTACC AGCTTTTAGC GGCAAAAAAG CTGATTCCCC GAAGGCAAGA 
GACGAGAAGA AAGAGCTGAA GATTGGGTCT GTGATCAGCG GCAAGCAGAT GAAGTCTGAT 
AAGAAGAAGG AAAGGAAGAA GGATGGTCAG GAGGTCCAGA ATTTGTCAGA GATGAACAAC 
AAGCTGAGCT CCATCATGGA CCACTGTAAC AATCAGATGG GTCAGATCGC CAGTCAGATC 
CAGAGCACCA CTGTGTTAAT CGGCAAGGAC CAGTTTGTAA AAGAGCTTCT TGGGAGAGCT 
ACTGTGAAGG GCAACGCTTC GCCACCTGGC ATCTCTACAC CGAAGAAACA CTGTGAAACA 
AAGGGCGACA TGCAGATCTG TCCAGATAAT ACCCTCATAC TGACGCAAAA GATCAGCCTC 
AAGGCCAAAA ACGACGAGGG GAACATTCCG GACACGTCTT GTCAAGACCA CATGATAGGC 
TCCAGCTGTC AGATGAGAAC CTTGGACAGC GGCATTGGAA CCTTCCCCCT GCCCGATTCG 
GTCACGCGGG CCAGCAGTCG CCACATCCCC AAATCTGAGT CCAGTCCAGA TGGGGTGATG 
GCCGGCTCAA CTGAGCGCAG TCGGGACACT CCCTCCTCCC ACCCTGACCC CTCACAGTCA 
GATTTCAAAG TGCCTTCCCC TTCAAAAGCC CATGTGCACA CCCCCACTAC CAGCATGGGT 
CACTCCCTGT CTGATCCCAC TGTGACATGC AGCAGGAATG CCCTGGAGAC CCAGAGTCGC 
CTGCCCAAGC TGGCATCCAC AGACGCAATC AAGACCAAAA GGTTGAGTCT TTGTGCCCAG 
CGCAGCGCCG TCTCCGCTTC TGCCGAGGGA GACGGGAAAG AAATGGAGAG GAAGATGAAA 
ACAAGCGAGC ATGAATCACC TGGTGAGACA ACATTGCGGG TTTGCGTGTA TCCAGGCAGC 
GACAGTGACA CGGAGACTGA ATCCGAGGGA ACTACAAGCA CTTTGGCGTC GCCGCTAAGA 
ACCCCCATGG ACAGACCTCA GAAGAACGAC TCTGTCGAGC AGAATGAGGA GACCTTGAAA 
AAACAGAATG CAGAGAAATC CCTGTCCATT ATGGACTACT ATCAGCACGA TGTGTTCTTG 
CACCTGGAGA CAGACGCCAG GAGGATTTCC CAGTACACCT TGCTGCACAA AGAGTCGTCC 
CTCGACGGAG AAGCAGGAGA CAGGCTCAGC AAAGAGATTC CTGTCAGTCC CAACCAGCCG 
GCCTCTTTGG ACGTCTCCTT GGAATCGTTG AACAAAATGA GCCACAGCAG CTCCAGCTGC 
CTGTGCGCGG ACGCCAGCCA CCGAGCCGGC TGCCCCGTAG AGACGGGGAG GACCGCAGAG 
AGCTGCTGCA AGGTCAGCCA GTCCTCCTGC TCCTCCAGCT TCATCAGCAG GCCCGGGGTG 
GACCCCGCGG GCTCCCTTAG CGACTCCCTC TACGACAGTT TCTCCTCCTG CACCAGTCAG 
GGCTCCAACG ATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020563985.1
CDS	442..6279
Translation	MDRHQERRERLKRLSLESRLPEYMDANKCIDELLKQLEEERRNVRREKLAVARLQREVARSKSEGTMREKLIHELEEERRLRLESEKRLREVTEESELGRAQMISLQQQFSRMEETVRSLLQNQGVLEQTAVDTVDIMKAYKDKLSEEVQKLQDGPEAKQAEPASTELDGSQADEDRDKTKLLLERLKTLEEENSALASENESQREQYERCLDEVANQVVQALLTQKDLREECLKLRTRVFDLEQQNRALSVLFQQKIKPASDLLLQKLHSRIMDLSAADLLLEPERSKAFLLSRNTDSPSNEVHSNGKPGVPVAKCLSQMSLMAPAPIYSRSSSELSLSSACSEFSSGSYTWNDGRSCGKMSSLTWEKRLSLGSSAPSNICAPLEEQPPTRRKESHILEGLRKLQRRKHRSSSCSSRVSKSGYKDCMNSNEGIYSLGIKSSSKGVSKPVHVSRASAALGRRFSYDSDDADDELARSSCRDNIPTKESWIHCKKLSHSISDSLCSLEGTQEAVGDGTSCPTASKPYDSRERPEKLMSFINSFLPLEGRKSAFSRPTVHHRNPTQVEDPSCRSNADDPEETIPDSVGVKTPCSQPQELAERDIKRLSRDTIKIFKQQFMQKDQGRTQSADGRPKPFSLLKELKATKCTQSEESILAIFDAEGEPIDLCAQRLVSGAEGRKAASCCSEFQKRTTRQNSANTTNYSVLESPEKPSEHQTRSGKGSDSMEGSAERLQLAPQRKLIKPPSSRTNKGFSIPPMSDVNSPKSGGSKIPGVNKPLASPLRVSKGTTTEPNNSGNSGPSAQEKSSSSLTVKMSRFIKTQGTSTQSPKAVNCKVSGQADLSKTSSSTSPHLPKRRLDFSDNSEQPTRDKHCETSKNKLRSPSPPPPPGRTTSLLVRPNYEASPQAHKSGAQPSTPTTVRGPPPSYHTSVAPNMQGTLPIKDKDCLDVDAGYGTALAPQKLVEKTTQHFQKTTAVTQTPTKGTSKRIKDYLPPASSGCARVPEIAPKNSKNVPPPYSALRVPSLQNSFANKKVPAQENTHQSVQKTSISLPISLQDSAQCQTEPQKAVMSPPGSAVTSPNLSEKASKTRIPMGFKAFLKSPPSHKSSPTIAGKQEKDHINSVSKETVTSNASTQCDTSQAAYSTDSTPTVSVPEGKGEFLCQPQKEDLQPPLSSAEGNVSDKAKRSSQLFGRSISINTKPHLKPALGMNGAKARSQSFSTNYIEKPNINAMEGPGRIRTQIITNSGERGNSLSRQSSSETTGVGLAESPIHAPRTRLSHHGGMMGPNSQSGLPEKSIQSCVIGEGKACGVKGDVVTSPSQREARSLPKSDRTAFINVQKPAKVDSHPQIQSSSAFVFSPENPTREAETITTTPAEPDKTQLGSPGNFPDTEEKKISPTTCTIEEKVMMGIEENLQKSQEQEKAAANEAKQKTGPSLANWFGLRKSKLPAFSGKKADSPKARDEKKELKIGSVISGKQMKSDKKKERKKDGQEVQNLSEMNNKLSSIMDHCNNQMGQIASQIQSTTVLIGKDQFVKELLGRATVKGNASPPGISTPKKHCETKGDMQICPDNTLILTQKISLKAKNDEGNIPDTSCQDHMIGSSCQMRTLDSGIGTFPLPDSVTRASSRHIPKSESSPDGVMAGSTERSRDTPSSHPDPSQSDFKVPSPSKAHVHTPTTSMGHSLSDPTVTCSRNALETQSRLPKLASTDAIKTKRLSLCAQRSAVSASAEGDGKEMERKMKTSEHESPGETTLRVCVYPGSDSDTETESEGTTSTLASPLRTPMDRPQKNDSVEQNEETLKKQNAEKSLSIMDYYQHDVFLHLETDARRISQYTLLHKESSLDGEAGDRLSKEIPVSPNQPASLDVSLESLNKMSHSSSSCLCADASHRAGCPVETGRTAESCCKVSQSSCSSSFISRPGVDPAGSLSDSLYDSFSSCTSQGSNDV

Target ORF information:

RefSeq Version	XM_020708326.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020708326.2

ORF Insert Sequence:

ATGGACAGGC ACCAGGAGAG GAGGGAACGT TTAAAAAGAT TGTCTCTGGA AAGCCGCCTG 
CCGGAGTACA TGGACGCCAA CAAATGCATC GATGAGCTTC TGAAACAGCT TGAGGAGGAG 
CGCCGGAATG TCCGAAGGGA AAAGCTGGCT GTGGCTCGCC TGCAGAGGGA AGTCGCACGA 
AGCAAGAGTG AAGGAACAAT GCGAGAAAAG TTGATCCACG AGCTAGAGGA AGAACGACGC 
CTGCGACTGG AGAGTGAGAA GCGTCTCCGG GAAGTGACGG AGGAGTCAGA GCTGGGAAGG 
GCGCAGATGA TTTCACTGCA GCAGCAGTTC TCCAGGATGG AAGAGACGGT GCGGTCGCTG 
CTGCAGAACC AGGGCGTCCT GGAGCAGACT GCTGTGGACA CAGTGGACAT CATGAAGGCC 
TACAAGGATA AACTCTCCGA GGAAGTGCAG AAACTGCAGG ATGGCCCAGA GGCGAAGCAG 
GCCGAGCCAG CGAGTACCGA GCTCGACGGC AGCCAAGCGG ACGAAGACAG AGACAAAACC 
AAGCTCCTTC TGGAGCGCCT GAAGACCCTC GAGGAGGAGA ACTCGGCGTT GGCCTCGGAG 
AACGAGAGCC AGAGGGAACA GTACGAGCGT TGCCTGGACG AGGTCGCTAA CCAAGTTGTA 
CAGGCACTCC TCACACAGAA GGATCTGAGA GAGGAATGTC TTAAGCTGCG GACAAGAGTT 
TTTGATCTGG AGCAGCAGAA TCGAGCACTG AGTGTTTTGT TCCAGCAGAA GATAAAGCCG 
GCATCAGACC TCCTCCTTCA GAAACTCCAC TCGCGGATCA TGGATCTGTC TGCGGCAGAT 
TTGCTTCTGG AGCCGGAGAG GAGCAAGGCC TTCCTCCTTT CCAGGAATAC AGACTCCCCT 
TCCAACGAGG TTCATTCGAA TGGAAAGCCA GGTGTCCCTG TGGCCAAATG TCTGAGCCAG 
ATGAGTCTGA TGGCGCCCGC TCCCATCTAT TCTCGCAGCA GCAGCGAGCT CTCACTGTCG 
AGCGCGTGCA GCGAGTTCTC CAGCGGCTCC TACACCTGGA ATGATGGGCG ATCCTGCGGG 
AAAATGTCAT CCCTGACGTG GGAGAAGAGA CTCAGTTTGG GCTCGTCAGC TCCAAGTAAC 
ATCTGCGCGC CGCTGGAGGA ACAGCCCCCC ACACGGCGTA AGGAGAGCCA CATACTGGAG 
GGCCTGAGAA AACTCCAGCG GAGGAAACAC AGGAGCTCCT CCTGCTCCTC TAGAGTGTCC 
AAGTCAGGCT ACAAAGATTG CATGAACTCC AACGAGGGCA TCTACTCTTT GGGTATCAAG 
AGCAGCAGCA AAGGGGTGTC TAAACCTGTA CATGTCAGCA GAGCTTCTGC TGCTCTTGGA 
AGGCGGTTTT CCTACGATTC CGATGATGCT GACGATGAAC TGGCACGCTC TAGTTGCAGA 
GATAATATTC CCACTAAGGA GAGCTGGATT CACTGCAAGA AGCTCTCCCA CAGCATCTCT 
GATAGTTTGT GCAGCTTGGA GGGGACACAG GAGGCTGTGG GTGATGGTAC TTCTTGTCCC 
ACAGCTTCAA AACCCTATGA CTCAAGGGAG CGTCCGGAGA AGCTCATGAG TTTCATCAAC 
AGTTTTCTTC CTTTAGAAGG GCGTAAATCA GCCTTTAGCA GGCCAACCGT GCACCACAGA 
AATCCTACTC AAGTAGAAGA TCCGAGTTGC AGATCCAACG CAGATGATCC AGAGGAAACC 
ATTCCTGATT CTGTGGGGGT CAAGACGCCA TGCAGTCAGC CGCAAGAACT AGCAGAGAGA 
GATATCAAGA GGCTCTCAAG GGATACCATC AAGATTTTCA AGCAACAGTT CATGCAAAAA 
GATCAGGGGC GCACTCAGTC TGCGGATGGG AGACCGAAGC CTTTCAGCCT ATTGAAGGAA 
CTTAAAGCAA CCAAGTGCAC CCAGTCTGAG GAGAGCATAC TGGCGATATT TGATGCTGAA 
GGAGAACCTA TTGATCTGTG CGCTCAGAGA CTAGTATCAG GAGCAGAAGG TCGCAAAGCC 
GCTAGCTGTT GCTCAGAGTT CCAAAAGAGG ACAACAAGGC AAAATTCAGC AAACACGACA 
AACTATAGCG TTCTTGAATC TCCAGAAAAA CCTTCAGAAC ATCAGACTAG ATCAGGCAAA 
GGCAGTGATA GCATGGAAGG AAGTGCAGAA AGGCTACAGT TAGCTCCTCA GAGGAAGTTG 
ATAAAGCCAC CAAGCAGTCG AACTAATAAA GGCTTTTCCA TTCCTCCCAT GAGTGACGTC 
AACAGCCCCA AATCTGGTGG CTCTAAGATC CCCGGTGTCA ATAAGCCCTT AGCTTCTCCG 
TTAAGAGTAT CCAAGGGCAC AACCACTGAG CCTAATAACA GTGGAAACTC AGGACCCTCT 
GCTCAGGAGA AATCTTCGTC TTCTCTTACG GTTAAGATGT CTAGATTCAT CAAGACGCAG 
GGAACTTCCA CTCAGAGTCC GAAAGCTGTG AACTGCAAGG TGTCTGGTCA GGCCGACTTA 
AGCAAGACCT CTTCTTCCAC TTCCCCCCAC CTTCCAAAAA GACGCCTCGA CTTCTCTGAC 
AACAGCGAAC AGCCGACCAG AGACAAACAC TGTGAAACCA GCAAGAACAA ACTCAGGTCT 
CCATCTCCTC CCCCTCCCCC AGGACGCACC ACCTCTTTAC TGGTCAGACC AAATTACGAA 
GCGTCGCCTC AAGCACACAA ATCAGGGGCT CAACCATCCA CGCCAACCAC TGTGAGGGGC 
CCTCCCCCAA GTTACCACAC CTCAGTTGCA CCAAATATGC AAGGCACGCT CCCCATCAAG 
GATAAAGACT GTTTAGACGT AGATGCAGGC TATGGGACTG CACTTGCACC TCAGAAGCTG 
GTGGAGAAAA CCACCCAGCA CTTTCAGAAG ACCACTGCCG TAACTCAGAC TCCAACTAAA 
GGCACTTCCA AGCGAATAAA AGACTACCTC CCACCTGCAA GCTCAGGATG TGCCAGAGTA 
CCTGAAATTG CACCTAAAAA CTCGAAGAAT GTCCCTCCTC CCTACAGTGC TCTAAGAGTG 
CCCTCCCTTC AAAACTCCTT TGCAAACAAA AAGGTACCTG CTCAAGAAAA CACCCATCAG 
TCAGTGCAAA AGACTTCCAT TAGTTTACCA ATATCGCTTC AGGATTCTGC TCAGTGTCAA 
ACAGAGCCAC AAAAAGCTGT CATGAGCCCT CCGGGCTCAG CTGTTACGTC ACCCAATTTG 
TCAGAAAAAG CCTCAAAGAC TCGAATCCCA ATGGGATTTA AAGCCTTTCT AAAATCCCCT 
CCCAGCCATA AAAGTAGTCC TACAATAGCA GGCAAGCAAG AGAAAGATCA TATCAACTCT 
GTCTCCAAGG AAACTGTGAC TTCAAATGCT TCTACTCAGT GTGACACCTC GCAGGCAGCG 
TACAGTACGG ATTCAACACC TACCGTGTCT GTTCCCGAAG GGAAAGGTGA ATTTCTGTGT 
CAGCCACAGA AAGAGGACTT ACAACCCCCC TTGTCGTCAG CAGAAGGAAA TGTGAGTGAC 
AAGGCAAAAA GGAGTAGTCA ACTCTTTGGC AGATCCATAT CTATTAATAC CAAACCACAC 
CTAAAGCCAG CTTTAGGGAT GAACGGGGCC AAAGCTCGAA GCCAGAGCTT CAGCACCAAC 
TACATAGAGA AACCCAACAT CAATGCCATG GAGGGGCCGG GAAGGATCAG GACTCAAATC 
ATCACTAACT CAGGTGAAAG GGGGAATTCG CTGTCAAGGC AGAGTTCTTC AGAAACAACT 
GGGGTTGGAT TGGCTGAAAG TCCAATCCAT GCCCCTAGAA CCCGGCTAAG CCATCATGGT 
GGCATGATGG GACCAAATAG TCAGAGCGGC CTCCCTGAGA AGTCCATTCA GTCGTGTGTT 
ATAGGTGAAG GAAAAGCTTG TGGAGTGAAA GGAGATGTGG TCACCTCTCC TTCTCAAAGA 
GAAGCTCGCA GCTTGCCCAA AAGTGACAGA ACTGCCTTTA TTAATGTCCA AAAGCCGGCA 
AAAGTGGACT CCCATCCTCA AATCCAGTCT TCCTCAGCTT TTGTCTTTAG TCCTGAAAAT 
CCTACCAGAG AAGCAGAAAC CATTACCACC ACTCCTGCTG AGCCAGACAA AACCCAGTTG 
GGTTCCCCAG GCAATTTCCC AGACACAGAG GAGAAAAAAA TCAGCCCAAC AACGTGCACT 
ATTGAAGAGA AAGTGATGAT GGGGATAGAA GAAAACCTGC AGAAAAGTCA AGAGCAGGAG 
AAGGCTGCAG CTAATGAGGC CAAACAGAAG ACGGGCCCGT CTTTGGCCAA CTGGTTTGGC 
CTCCGCAAGA GCAAACTACC AGCTTTTAGC GGCAAAAAAG CTGATTCCCC GAAGGCAAGA 
GACGAGAAGA AAGAGCTGAA GATTGGGTCT GTGATCAGCG GCAAGCAGAT GAAGTCTGAT 
AAGAAGAAGG AAAGGAAGAA GGATGGTCAG GAGGTCCAGA ATTTGTCAGA GATGAACAAC 
AAGCTGAGCT CCATCATGGA CCACTGTAAC AATCAGATGG GTCAGATCGC CAGTCAGATC 
CAGAGCACCA CTGTGTTAAT CGGCAAGGAC CAGTTTGTAA AAGAGCTTCT TGGGAGAGCT 
ACTGTGAAGG GCAACGCTTC GCCACCTGGC ATCTCTACAC CGAAGAAACA CTGTGAAACA 
AAGGGCGACA TGCAGATCTG TCCAGATAAT ACCCTCATAC TGACGCAAAA GATCAGCCTC 
AAGGCCAAAA ACGACGAGGG GAACATTCCG GACACGTCTT GTCAAGACCA CATGATAGGC 
TCCAGCTGTC AGATGAGAAC CTTGGACAGC GGCATTGGAA CCTTCCCCCT GCCCGATTCG 
GTCACGCGGG CCAGCAGTCG CCACATCCCC AAATCTGAGT CCAGTCCAGA TGGGGTGATG 
GCCGGCTCAA CTGAGCGCAG TCGGGACACT CCCTCCTCCC ACCCTGACCC CTCACAGTCA 
GATTTCAAAG TGCCTTCCCC TTCAAAAGCC CATGTGCACA CCCCCACTAC CAGCATGGGT 
CACTCCCTGT CTGATCCCAC TGTGACATGC AGCAGGAATG CCCTGGAGAC CCAGAGTCGC 
CTGCCCAAGC TGGCATCCAC AGACGCAATC AAGACCAAAA GGTTGAGTCT TTGTGCCCAG 
CGCAGCGCCG TCTCCGCTTC TGCCGAGGGA GACGGGAAAG AAATGGAGAG GAAGATGAAA 
ACAAGCGAGC ATGAATCACC TGGTGAGACA ACATTGCGGG TTTGCGTGTA TCCAGGCAGC 
GACAGTGACA CGGAGACTGA ATCCGAGGGA ACTACAAGCA CTTTGGCGTC GCCGCTAAGA 
ACCCCCATGG ACAGACCTCA GAAGAACGAC TCTGTCGAGC AGAATGAGGA GACCTTGAAA 
AAACAGAATG CAGAGAAATC CCTGTCCATT ATGGACTACT ATCAGCACGA TGTGTTCTTG 
CACCTGGAGA CAGACGCCAG GAGGATTTCC CAGTACACCT TGCTGCACAA AGAGTCGTCC 
CTCGACGGAG AAGCAGGAGA CAGGCTCAGC AAAGAGATTC CTGTCAGTCC CAACCAGCCG 
GCCTCTTTGG ACGTCTCCTT GGAATCGTTG AACAAAATGA GCCACAGCAG CTCCAGCTGC 
CTGTGCGCGG ACGCCAGCCA CCGAGCCGGC TGCCCCGTAG AGACGGGGAG GACCGCAGAG 
AGCTGCTGCA AGGTCAGCCA GTCCTCCTGC TCCTCCAGCT TCATCAGCAG GCCCGGGGTG 
GACCCCGCGG GCTCCCTTAG CGACTCCCTC TACGACAGTT TCTCCTCCTG CACCAGTCAG 
GGCTCCAACG ATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101162908 ( XM_020708323.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020563982.1 Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X1, mRNA.

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CloneID	OJa74623
Clone ID Related Accession (Same CDS sequence)	XM_020708323.1 , XM_020708323.2
Accession Version	XM_020708323.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	nck-associated protein 5-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGT TTACAGATCA CAGTGTCGCG TCGGTCTGCA GGAGGATCCG AAACATTCCC 
CTGCTGGAGC TGCTGGCGGA GTACATGGAC GCCAACAAAT GCATCGATGA GCTTCTGAAA 
CAGCTTGAGG AGGAGCGCCG GAATGTCCGA AGGGAAAAGC TGGCTGTGGC TCGCCTGCAG 
AGGGAAGTCG CACGAAGCAA GAGTGAAGGA ACAATGCGAG AAAAGTTGAT CCACGAGCTA 
GAGGAAGAAC GACGCCTGCG ACTGGAGAGT GAGAAGCGTC TCCGGGAAGT GACGGAGGAG 
TCAGAGCTGG GAAGGGCGCA GATGATTTCA CTGCAGCAGC AGTTCTCCAG GATGGAAGAG 
ACGGTGCGGT CGCTGCTGCA GAACCAGGGC GTCCTGGAGC AGACTGCTGT GGACACAGTG 
GACATCATGA AGGCCTACAA GGATAAACTC TCCGAGGAAG TGCAGAAACT GCAGGATGGC 
CCAGAGGCGA AGCAGGCCGA GCCAGCGAGT ACCGAGCTCG ACGGCAGCCA AGCGGACGAA 
GACAGAGACA AAACCAAGCT CCTTCTGGAG CGCCTGAAGA CCCTCGAGGA GGAGAACTCG 
GCGTTGGCCT CGGAGAACGA GAGCCAGAGG GAACAGTACG AGCGTTGCCT GGACGAGGTC 
GCTAACCAAG TTGTACAGGC ACTCCTCACA CAGAAGGATC TGAGAGAGGA ATGTCTTAAG 
CTGCGGACAA GAGTTTTTGA TCTGGAGCAG CAGAATCGAG CACTGAGTGT TTTGTTCCAG 
CAGAAGATAA AGCCGGCATC AGACCTCCTC CTTCAGAAAC TCCACTCGCG GATCATGGAT 
CTGTCTGCGG CAGATTTGCT TCTGGAGCCG GAGAGGAGCA AGGCCTTCCT CCTTTCCAGG 
AATACAGACT CCCCTTCCAA CGAGGTTCAT TCGAATGGAA AGCCAGGTGT CCCTGTGGCC 
AAATGTCTGA GCCAGATGAG TCTGATGGCG CCCGCTCCCA TCTATTCTCG CAGCAGCAGC 
GAGCTCTCAC TGTCGAGCGC GTGCAGCGAG TTCTCCAGCG GCTCCTACAC CTGGAATGAT 
GGGCGATCCT GCGGGAAAAT GTCATCCCTG ACGTGGGAGA AGAGACTCAG TTTGGGCTCG 
TCAGCTCCAA GTAACATCTG CGCGCCGCTG GAGGAACAGC CCCCCACACG GCGTAAGGAG 
AGCCACATAC TGGAGGGCCT GAGAAAACTC CAGCGGAGGA AACACAGGAG CTCCTCCTGC 
TCCTCTAGAG TGTCCAAGTC AGGCTACAAA GATTGCATGA ACTCCAACGA GGGCATCTAC 
TCTTTGGGTA TCAAGAGCAG CAGCAAAGGG GTGTCTAAAC CTGTACATGT CAGCAGAGCT 
TCTGCTGCTC TTGGAAGGCG GTTTTCCTAC GATTCCGATG ATGCTGACGA TGAACTGGCA 
CGCTCTAGTT GCAGAGATAA TATTCCCACT AAGGAGAGCT GGATTCACTG CAAGAAGCTC 
TCCCACAGCA TCTCTGATAG TTTGTGCAGC TTGGAGGGGA CACAGGAGGC TGTGGGTGAT 
GGTACTTCTT GTCCCACAGC TTCAAAACCC TATGACTCAA GGGAGCGTCC GGAGAAGCTC 
ATGAGTTTCA TCAACAGTTT TCTTCCTTTA GAAGGGCGTA AATCAGCCTT TAGCAGGCCA 
ACCGTGCACC ACAGAAATCC TACTCAAGTA GAAGATCCGA GTTGCAGATC CAACGCAGAT 
GATCCAGAGG AAACCATTCC TGATTCTGTG GGGGTCAAGA CGCCATGCAG TCAGCCGCAA 
GAACTAGCAG AGAGAGATAT CAAGAGGCTC TCAAGGGATA CCATCAAGAT TTTCAAGCAA 
CAGTTCATGC AAAAAGATCA GGGGCGCACT CAGTCTGCGG ATGGGAGACC GAAGCCTTTC 
AGCCTATTGA AGGAACTTAA AGCAACCAAG TGCACCCAGT CTGAGGAGAG CATACTGGCG 
ATATTTGATG CTGAAGGAGA ACCTATTGAT CTGTGCGCTC AGAGACTAGT ATCAGGAGCA 
GAAGGTCGCA AAGCCGCTAG CTGTTGCTCA GAGTTCCAAA AGAGGACAAC AAGGCAAAAT 
TCAGCAAACA CGACAAACTA TAGCGTTCTT GAATCTCCAG AAAAACCTTC AGAACATCAG 
ACTAGATCAG GCAAAGGCAG TGATAGCATG GAAGGAAGTG CAGAAAGGCT ACAGTTAGCT 
CCTCAGAGGA AGTTGATAAA GCCACCAAGC AGTCGAACTA ATAAAGGCTT TTCCATTCCT 
CCCATGAGTG ACGTCAACAG CCCCAAATCT GGTGGCTCTA AGATCCCCGG TGTCAATAAG 
CCCTTAGCTT CTCCGTTAAG AGTATCCAAG GGCACAACCA CTGAGCCTAA TAACAGTGGA 
AACTCAGGAC CCTCTGCTCA GGAGAAATCT TCGTCTTCTC TTACGGTTAA GATGTCTAGA 
TTCATCAAGA CGCAGGGAAC TTCCACTCAG AGTCCGAAAG CTGTGAACTG CAAGGTGTCT 
GGTCAGGCCG ACTTAAGCAA GACCTCTTCT TCCACTTCCC CCCACCTTCC AAAAAGACGC 
CTCGACTTCT CTGACAACAG CGAACAGCCG ACCAGAGACA AACACTGTGA AACCAGCAAG 
AACAAACTCA GGTCTCCATC TCCTCCCCCT CCCCCAGGAC GCACCACCTC TTTACTGGTC 
AGACCAAATT ACGAAGCGTC GCCTCAAGCA CACAAATCAG GGGCTCAACC ATCCACGCCA 
ACCACTGTGA GGGGCCCTCC CCCAAGTTAC CACACCTCAG TTGCACCAAA TATGCAAGGC 
ACGCTCCCCA TCAAGGATAA AGACTGTTTA GACGTAGATG CAGGCTATGG GACTGCACTT 
GCACCTCAGA AGCTGGTGGA GAAAACCACC CAGCACTTTC AGAAGACCAC TGCCGTAACT 
CAGACTCCAA CTAAAGGCAC TTCCAAGCGA ATAAAAGACT ACCTCCCACC TGCAAGCTCA 
GGATGTGCCA GAGTACCTGA AATTGCACCT AAAAACTCGA AGAATGTCCC TCCTCCCTAC 
AGTGCTCTAA GAGTGCCCTC CCTTCAAAAC TCCTTTGCAA ACAAAAAGGT ACCTGCTCAA 
GAAAACACCC ATCAGTCAGT GCAAAAGACT TCCATTAGTT TACCAATATC GCTTCAGGAT 
TCTGCTCAGT GTCAAACAGA GCCACAAAAA GCTGTCATGA GCCCTCCGGG CTCAGCTGTT 
ACGTCACCCA ATTTGTCAGA AAAAGCCTCA AAGACTCGAA TCCCAATGGG ATTTAAAGCC 
TTTCTAAAAT CCCCTCCCAG CCATAAAAGT AGTCCTACAA TAGCAGGCAA GCAAGAGAAA 
GATCATATCA ACTCTGTCTC CAAGGAAACT GTGACTTCAA ATGCTTCTAC TCAGTGTGAC 
ACCTCGCAGG CAGCGTACAG TACGGATTCA ACACCTACCG TGTCTGTTCC CGAAGGGAAA 
GGTGAATTTC TGTGTCAGCC ACAGAAAGAG GACTTACAAC CCCCCTTGTC GTCAGCAGAA 
GGAAATGTGA GTGACAAGGC AAAAAGGAGT AGTCAACTCT TTGGCAGATC CATATCTATT 
AATACCAAAC CACACCTAAA GCCAGCTTTA GGGATGAACG GGGCCAAAGC TCGAAGCCAG 
AGCTTCAGCA CCAACTACAT AGAGAAACCC AACATCAATG CCATGGAGGG GCCGGGAAGG 
ATCAGGACTC AAATCATCAC TAACTCAGGT GAAAGGGGGA ATTCGCTGTC AAGGCAGAGT 
TCTTCAGAAA CAACTGGGGT TGGATTGGCT GAAAGTCCAA TCCATGCCCC TAGAACCCGG 
CTAAGCCATC ATGGTGGCAT GATGGGACCA AATAGTCAGA GCGGCCTCCC TGAGAAGTCC 
ATTCAGTCGT GTGTTATAGG TGAAGGAAAA GCTTGTGGAG TGAAAGGAGA TGTGGTCACC 
TCTCCTTCTC AAAGAGAAGC TCGCAGCTTG CCCAAAAGTG ACAGAACTGC CTTTATTAAT 
GTCCAAAAGC CGGCAAAAGT GGACTCCCAT CCTCAAATCC AGTCTTCCTC AGCTTTTGTC 
TTTAGTCCTG AAAATCCTAC CAGAGAAGCA GAAACCATTA CCACCACTCC TGCTGAGCCA 
GACAAAACCC AGTTGGGTTC CCCAGGCAAT TTCCCAGACA CAGAGGAGAA AAAAATCAGC 
CCAACAACGT GCACTATTGA AGAGAAAGTG ATGATGGGGA TAGAAGAAAA CCTGCAGAAA 
AGTCAAGAGC AGGAGAAGGC TGCAGCTAAT GAGGCCAAAC AGAAGACGGG CCCGTCTTTG 
GCCAACTGGT TTGGCCTCCG CAAGAGCAAA CTACCAGCTT TTAGCGGCAA AAAAGCTGAT 
TCCCCGAAGG CAAGAGACGA GAAGAAAGAG CTGAAGATTG GGTCTGTGAT CAGCGGCAAG 
CAGATGAAGT CTGATAAGAA GAAGGAAAGG AAGAAGGATG GTCAGGAGGT CCAGAATTTG 
TCAGAGATGA ACAACAAGCT GAGCTCCATC ATGGACCACT GTAACAATCA GATGGGTCAG 
ATCGCCAGTC AGATCCAGAG CACCACTGTG TTAATCGGCA AGGACCAGTT TGTAAAAGAG 
CTTCTTGGGA GAGCTACTGT GAAGGGCAAC GCTTCGCCAC CTGGCATCTC TACACCGAAG 
AAACACTGTG AAACAAAGGG CGACATGCAG ATCTGTCCAG ATAATACCCT CATACTGACG 
CAAAAGATCA GCCTCAAGGC CAAAAACGAC GAGGGGAACA TTCCGGACAC GTCTTGTCAA 
GACCACATGA TAGGCTCCAG CTGTCAGATG AGAACCTTGG ACAGCGGCAT TGGAACCTTC 
CCCCTGCCCG ATTCGGTCAC GCGGGCCAGC AGTCGCCACA TCCCCAAATC TGAGTCCAGT 
CCAGATGGGG TGATGGCCGG CTCAACTGAG CGCAGTCGGG ACACTCCCTC CTCCCACCCT 
GACCCCTCAC AGTCAGATTT CAAAGTGCCT TCCCCTTCAA AAGCCCATGT GCACACCCCC 
ACTACCAGCA TGGGTCACTC CCTGTCTGAT CCCACTGTGA CATGCAGCAG GAATGCCCTG 
GAGACCCAGA GTCGCCTGCC CAAGCTGGCA TCCACAGACG CAATCAAGAC CAAAAGGTTG 
AGTCTTTGTG CCCAGCGCAG CGCCGTCTCC GCTTCTGCCG AGGGAGACGG GAAAGAAATG 
GAGAGGAAGA TGAAAACAAG CGAGCATGAA TCACCTGGTG AGACAACATT GCGGGTTTGC 
GTGTATCCAG GCAGCGACAG TGACACGGAG ACTGAATCCG AGGGAACTAC AAGCACTTTG 
GCGTCGCCGC TAAGAACCCC CATGGACAGA CCTCAGAAGA ACGACTCTGT CGAGCAGAAT 
GAGGAGACCT TGAAAAAACA GAATGCAGAG AAATCCCTGT CCATTATGGA CTACTATCAG 
CACGATGTGT TCTTGCACCT GGAGACAGAC GCCAGGAGGA TTTCCCAGTA CACCTTGCTG 
CACAAAGAGT CGTCCCTCGA CGGAGAAGCA GGAGACAGGC TCAGCAAAGA GATTCCTGTC 
AGTCCCAACC AGCCGGCCTC TTTGGACGTC TCCTTGGAAT CGTTGAACAA AATGAGCCAC 
AGCAGCTCCA GCTGCCTGTG CGCGGACGCC AGCCACCGAG CCGGCTGCCC CGTAGAGACG 
GGGAGGACCG CAGAGAGCTG CTGCAAGGTC AGCCAGTCCT CCTGCTCCTC CAGCTTCATC 
AGCAGGCCCG GGGTGGACCC CGCGGGCTCC CTTAGCGACT CCCTCTACGA CAGTTTCTCC 
TCCTGCACCA GTCAGGGCTC CAACGATGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020563982.1
CDS	117..5969
Translation	MSQFTDHSVASVCRRIRNIPLLELLAEYMDANKCIDELLKQLEEERRNVRREKLAVARLQREVARSKSEGTMREKLIHELEEERRLRLESEKRLREVTEESELGRAQMISLQQQFSRMEETVRSLLQNQGVLEQTAVDTVDIMKAYKDKLSEEVQKLQDGPEAKQAEPASTELDGSQADEDRDKTKLLLERLKTLEEENSALASENESQREQYERCLDEVANQVVQALLTQKDLREECLKLRTRVFDLEQQNRALSVLFQQKIKPASDLLLQKLHSRIMDLSAADLLLEPERSKAFLLSRNTDSPSNEVHSNGKPGVPVAKCLSQMSLMAPAPIYSRSSSELSLSSACSEFSSGSYTWNDGRSCGKMSSLTWEKRLSLGSSAPSNICAPLEEQPPTRRKESHILEGLRKLQRRKHRSSSCSSRVSKSGYKDCMNSNEGIYSLGIKSSSKGVSKPVHVSRASAALGRRFSYDSDDADDELARSSCRDNIPTKESWIHCKKLSHSISDSLCSLEGTQEAVGDGTSCPTASKPYDSRERPEKLMSFINSFLPLEGRKSAFSRPTVHHRNPTQVEDPSCRSNADDPEETIPDSVGVKTPCSQPQELAERDIKRLSRDTIKIFKQQFMQKDQGRTQSADGRPKPFSLLKELKATKCTQSEESILAIFDAEGEPIDLCAQRLVSGAEGRKAASCCSEFQKRTTRQNSANTTNYSVLESPEKPSEHQTRSGKGSDSMEGSAERLQLAPQRKLIKPPSSRTNKGFSIPPMSDVNSPKSGGSKIPGVNKPLASPLRVSKGTTTEPNNSGNSGPSAQEKSSSSLTVKMSRFIKTQGTSTQSPKAVNCKVSGQADLSKTSSSTSPHLPKRRLDFSDNSEQPTRDKHCETSKNKLRSPSPPPPPGRTTSLLVRPNYEASPQAHKSGAQPSTPTTVRGPPPSYHTSVAPNMQGTLPIKDKDCLDVDAGYGTALAPQKLVEKTTQHFQKTTAVTQTPTKGTSKRIKDYLPPASSGCARVPEIAPKNSKNVPPPYSALRVPSLQNSFANKKVPAQENTHQSVQKTSISLPISLQDSAQCQTEPQKAVMSPPGSAVTSPNLSEKASKTRIPMGFKAFLKSPPSHKSSPTIAGKQEKDHINSVSKETVTSNASTQCDTSQAAYSTDSTPTVSVPEGKGEFLCQPQKEDLQPPLSSAEGNVSDKAKRSSQLFGRSISINTKPHLKPALGMNGAKARSQSFSTNYIEKPNINAMEGPGRIRTQIITNSGERGNSLSRQSSSETTGVGLAESPIHAPRTRLSHHGGMMGPNSQSGLPEKSIQSCVIGEGKACGVKGDVVTSPSQREARSLPKSDRTAFINVQKPAKVDSHPQIQSSSAFVFSPENPTREAETITTTPAEPDKTQLGSPGNFPDTEEKKISPTTCTIEEKVMMGIEENLQKSQEQEKAAANEAKQKTGPSLANWFGLRKSKLPAFSGKKADSPKARDEKKELKIGSVISGKQMKSDKKKERKKDGQEVQNLSEMNNKLSSIMDHCNNQMGQIASQIQSTTVLIGKDQFVKELLGRATVKGNASPPGISTPKKHCETKGDMQICPDNTLILTQKISLKAKNDEGNIPDTSCQDHMIGSSCQMRTLDSGIGTFPLPDSVTRASSRHIPKSESSPDGVMAGSTERSRDTPSSHPDPSQSDFKVPSPSKAHVHTPTTSMGHSLSDPTVTCSRNALETQSRLPKLASTDAIKTKRLSLCAQRSAVSASAEGDGKEMERKMKTSEHESPGETTLRVCVYPGSDSDTETESEGTTSTLASPLRTPMDRPQKNDSVEQNEETLKKQNAEKSLSIMDYYQHDVFLHLETDARRISQYTLLHKESSLDGEAGDRLSKEIPVSPNQPASLDVSLESLNKMSHSSSSCLCADASHRAGCPVETGRTAESCCKVSQSSCSSSFISRPGVDPAGSLSDSLYDSFSSCTSQGSNDV

Target ORF information:

RefSeq Version	XM_020708323.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020708323.1

ORF Insert Sequence:

ATGAGCCAGT TTACAGATCA CAGTGTCGCG TCGGTCTGCA GGAGGATCCG AAACATTCCC 
CTGCTGGAGC TGCTGGCGGA GTACATGGAC GCCAACAAAT GCATCGATGA GCTTCTGAAA 
CAGCTTGAGG AGGAGCGCCG GAATGTCCGA AGGGAAAAGC TGGCTGTGGC TCGCCTGCAG 
AGGGAAGTCG CACGAAGCAA GAGTGAAGGA ACAATGCGAG AAAAGTTGAT CCACGAGCTA 
GAGGAAGAAC GACGCCTGCG ACTGGAGAGT GAGAAGCGTC TCCGGGAAGT GACGGAGGAG 
TCAGAGCTGG GAAGGGCGCA GATGATTTCA CTGCAGCAGC AGTTCTCCAG GATGGAAGAG 
ACGGTGCGGT CGCTGCTGCA GAACCAGGGC GTCCTGGAGC AGACTGCTGT GGACACAGTG 
GACATCATGA AGGCCTACAA GGATAAACTC TCCGAGGAAG TGCAGAAACT GCAGGATGGC 
CCAGAGGCGA AGCAGGCCGA GCCAGCGAGT ACCGAGCTCG ACGGCAGCCA AGCGGACGAA 
GACAGAGACA AAACCAAGCT CCTTCTGGAG CGCCTGAAGA CCCTCGAGGA GGAGAACTCG 
GCGTTGGCCT CGGAGAACGA GAGCCAGAGG GAACAGTACG AGCGTTGCCT GGACGAGGTC 
GCTAACCAAG TTGTACAGGC ACTCCTCACA CAGAAGGATC TGAGAGAGGA ATGTCTTAAG 
CTGCGGACAA GAGTTTTTGA TCTGGAGCAG CAGAATCGAG CACTGAGTGT TTTGTTCCAG 
CAGAAGATAA AGCCGGCATC AGACCTCCTC CTTCAGAAAC TCCACTCGCG GATCATGGAT 
CTGTCTGCGG CAGATTTGCT TCTGGAGCCG GAGAGGAGCA AGGCCTTCCT CCTTTCCAGG 
AATACAGACT CCCCTTCCAA CGAGGTTCAT TCGAATGGAA AGCCAGGTGT CCCTGTGGCC 
AAATGTCTGA GCCAGATGAG TCTGATGGCG CCCGCTCCCA TCTATTCTCG CAGCAGCAGC 
GAGCTCTCAC TGTCGAGCGC GTGCAGCGAG TTCTCCAGCG GCTCCTACAC CTGGAATGAT 
GGGCGATCCT GCGGGAAAAT GTCATCCCTG ACGTGGGAGA AGAGACTCAG TTTGGGCTCG 
TCAGCTCCAA GTAACATCTG CGCGCCGCTG GAGGAACAGC CCCCCACACG GCGTAAGGAG 
AGCCACATAC TGGAGGGCCT GAGAAAACTC CAGCGGAGGA AACACAGGAG CTCCTCCTGC 
TCCTCTAGAG TGTCCAAGTC AGGCTACAAA GATTGCATGA ACTCCAACGA GGGCATCTAC 
TCTTTGGGTA TCAAGAGCAG CAGCAAAGGG GTGTCTAAAC CTGTACATGT CAGCAGAGCT 
TCTGCTGCTC TTGGAAGGCG GTTTTCCTAC GATTCCGATG ATGCTGACGA TGAACTGGCA 
CGCTCTAGTT GCAGAGATAA TATTCCCACT AAGGAGAGCT GGATTCACTG CAAGAAGCTC 
TCCCACAGCA TCTCTGATAG TTTGTGCAGC TTGGAGGGGA CACAGGAGGC TGTGGGTGAT 
GGTACTTCTT GTCCCACAGC TTCAAAACCC TATGACTCAA GGGAGCGTCC GGAGAAGCTC 
ATGAGTTTCA TCAACAGTTT TCTTCCTTTA GAAGGGCGTA AATCAGCCTT TAGCAGGCCA 
ACCGTGCACC ACAGAAATCC TACTCAAGTA GAAGATCCGA GTTGCAGATC CAACGCAGAT 
GATCCAGAGG AAACCATTCC TGATTCTGTG GGGGTCAAGA CGCCATGCAG TCAGCCGCAA 
GAACTAGCAG AGAGAGATAT CAAGAGGCTC TCAAGGGATA CCATCAAGAT TTTCAAGCAA 
CAGTTCATGC AAAAAGATCA GGGGCGCACT CAGTCTGCGG ATGGGAGACC GAAGCCTTTC 
AGCCTATTGA AGGAACTTAA AGCAACCAAG TGCACCCAGT CTGAGGAGAG CATACTGGCG 
ATATTTGATG CTGAAGGAGA ACCTATTGAT CTGTGCGCTC AGAGACTAGT ATCAGGAGCA 
GAAGGTCGCA AAGCCGCTAG CTGTTGCTCA GAGTTCCAAA AGAGGACAAC AAGGCAAAAT 
TCAGCAAACA CGACAAACTA TAGCGTTCTT GAATCTCCAG AAAAACCTTC AGAACATCAG 
ACTAGATCAG GCAAAGGCAG TGATAGCATG GAAGGAAGTG CAGAAAGGCT ACAGTTAGCT 
CCTCAGAGGA AGTTGATAAA GCCACCAAGC AGTCGAACTA ATAAAGGCTT TTCCATTCCT 
CCCATGAGTG ACGTCAACAG CCCCAAATCT GGTGGCTCTA AGATCCCCGG TGTCAATAAG 
CCCTTAGCTT CTCCGTTAAG AGTATCCAAG GGCACAACCA CTGAGCCTAA TAACAGTGGA 
AACTCAGGAC CCTCTGCTCA GGAGAAATCT TCGTCTTCTC TTACGGTTAA GATGTCTAGA 
TTCATCAAGA CGCAGGGAAC TTCCACTCAG AGTCCGAAAG CTGTGAACTG CAAGGTGTCT 
GGTCAGGCCG ACTTAAGCAA GACCTCTTCT TCCACTTCCC CCCACCTTCC AAAAAGACGC 
CTCGACTTCT CTGACAACAG CGAACAGCCG ACCAGAGACA AACACTGTGA AACCAGCAAG 
AACAAACTCA GGTCTCCATC TCCTCCCCCT CCCCCAGGAC GCACCACCTC TTTACTGGTC 
AGACCAAATT ACGAAGCGTC GCCTCAAGCA CACAAATCAG GGGCTCAACC ATCCACGCCA 
ACCACTGTGA GGGGCCCTCC CCCAAGTTAC CACACCTCAG TTGCACCAAA TATGCAAGGC 
ACGCTCCCCA TCAAGGATAA AGACTGTTTA GACGTAGATG CAGGCTATGG GACTGCACTT 
GCACCTCAGA AGCTGGTGGA GAAAACCACC CAGCACTTTC AGAAGACCAC TGCCGTAACT 
CAGACTCCAA CTAAAGGCAC TTCCAAGCGA ATAAAAGACT ACCTCCCACC TGCAAGCTCA 
GGATGTGCCA GAGTACCTGA AATTGCACCT AAAAACTCGA AGAATGTCCC TCCTCCCTAC 
AGTGCTCTAA GAGTGCCCTC CCTTCAAAAC TCCTTTGCAA ACAAAAAGGT ACCTGCTCAA 
GAAAACACCC ATCAGTCAGT GCAAAAGACT TCCATTAGTT TACCAATATC GCTTCAGGAT 
TCTGCTCAGT GTCAAACAGA GCCACAAAAA GCTGTCATGA GCCCTCCGGG CTCAGCTGTT 
ACGTCACCCA ATTTGTCAGA AAAAGCCTCA AAGACTCGAA TCCCAATGGG ATTTAAAGCC 
TTTCTAAAAT CCCCTCCCAG CCATAAAAGT AGTCCTACAA TAGCAGGCAA GCAAGAGAAA 
GATCATATCA ACTCTGTCTC CAAGGAAACT GTGACTTCAA ATGCTTCTAC TCAGTGTGAC 
ACCTCGCAGG CAGCGTACAG TACGGATTCA ACACCTACCG TGTCTGTTCC CGAAGGGAAA 
GGTGAATTTC TGTGTCAGCC ACAGAAAGAG GACTTACAAC CCCCCTTGTC GTCAGCAGAA 
GGAAATGTGA GTGACAAGGC AAAAAGGAGT AGTCAACTCT TTGGCAGATC CATATCTATT 
AATACCAAAC CACACCTAAA GCCAGCTTTA GGGATGAACG GGGCCAAAGC TCGAAGCCAG 
AGCTTCAGCA CCAACTACAT AGAGAAACCC AACATCAATG CCATGGAGGG GCCGGGAAGG 
ATCAGGACTC AAATCATCAC TAACTCAGGT GAAAGGGGGA ATTCGCTGTC AAGGCAGAGT 
TCTTCAGAAA CAACTGGGGT TGGATTGGCT GAAAGTCCAA TCCATGCCCC TAGAACCCGG 
CTAAGCCATC ATGGTGGCAT GATGGGACCA AATAGTCAGA GCGGCCTCCC TGAGAAGTCC 
ATTCAGTCGT GTGTTATAGG TGAAGGAAAA GCTTGTGGAG TGAAAGGAGA TGTGGTCACC 
TCTCCTTCTC AAAGAGAAGC TCGCAGCTTG CCCAAAAGTG ACAGAACTGC CTTTATTAAT 
GTCCAAAAGC CGGCAAAAGT GGACTCCCAT CCTCAAATCC AGTCTTCCTC AGCTTTTGTC 
TTTAGTCCTG AAAATCCTAC CAGAGAAGCA GAAACCATTA CCACCACTCC TGCTGAGCCA 
GACAAAACCC AGTTGGGTTC CCCAGGCAAT TTCCCAGACA CAGAGGAGAA AAAAATCAGC 
CCAACAACGT GCACTATTGA AGAGAAAGTG ATGATGGGGA TAGAAGAAAA CCTGCAGAAA 
AGTCAAGAGC AGGAGAAGGC TGCAGCTAAT GAGGCCAAAC AGAAGACGGG CCCGTCTTTG 
GCCAACTGGT TTGGCCTCCG CAAGAGCAAA CTACCAGCTT TTAGCGGCAA AAAAGCTGAT 
TCCCCGAAGG CAAGAGACGA GAAGAAAGAG CTGAAGATTG GGTCTGTGAT CAGCGGCAAG 
CAGATGAAGT CTGATAAGAA GAAGGAAAGG AAGAAGGATG GTCAGGAGGT CCAGAATTTG 
TCAGAGATGA ACAACAAGCT GAGCTCCATC ATGGACCACT GTAACAATCA GATGGGTCAG 
ATCGCCAGTC AGATCCAGAG CACCACTGTG TTAATCGGCA AGGACCAGTT TGTAAAAGAG 
CTTCTTGGGA GAGCTACTGT GAAGGGCAAC GCTTCGCCAC CTGGCATCTC TACACCGAAG 
AAACACTGTG AAACAAAGGG CGACATGCAG ATCTGTCCAG ATAATACCCT CATACTGACG 
CAAAAGATCA GCCTCAAGGC CAAAAACGAC GAGGGGAACA TTCCGGACAC GTCTTGTCAA 
GACCACATGA TAGGCTCCAG CTGTCAGATG AGAACCTTGG ACAGCGGCAT TGGAACCTTC 
CCCCTGCCCG ATTCGGTCAC GCGGGCCAGC AGTCGCCACA TCCCCAAATC TGAGTCCAGT 
CCAGATGGGG TGATGGCCGG CTCAACTGAG CGCAGTCGGG ACACTCCCTC CTCCCACCCT 
GACCCCTCAC AGTCAGATTT CAAAGTGCCT TCCCCTTCAA AAGCCCATGT GCACACCCCC 
ACTACCAGCA TGGGTCACTC CCTGTCTGAT CCCACTGTGA CATGCAGCAG GAATGCCCTG 
GAGACCCAGA GTCGCCTGCC CAAGCTGGCA TCCACAGACG CAATCAAGAC CAAAAGGTTG 
AGTCTTTGTG CCCAGCGCAG CGCCGTCTCC GCTTCTGCCG AGGGAGACGG GAAAGAAATG 
GAGAGGAAGA TGAAAACAAG CGAGCATGAA TCACCTGGTG AGACAACATT GCGGGTTTGC 
GTGTATCCAG GCAGCGACAG TGACACGGAG ACTGAATCCG AGGGAACTAC AAGCACTTTG 
GCGTCGCCGC TAAGAACCCC CATGGACAGA CCTCAGAAGA ACGACTCTGT CGAGCAGAAT 
GAGGAGACCT TGAAAAAACA GAATGCAGAG AAATCCCTGT CCATTATGGA CTACTATCAG 
CACGATGTGT TCTTGCACCT GGAGACAGAC GCCAGGAGGA TTTCCCAGTA CACCTTGCTG 
CACAAAGAGT CGTCCCTCGA CGGAGAAGCA GGAGACAGGC TCAGCAAAGA GATTCCTGTC 
AGTCCCAACC AGCCGGCCTC TTTGGACGTC TCCTTGGAAT CGTTGAACAA AATGAGCCAC 
AGCAGCTCCA GCTGCCTGTG CGCGGACGCC AGCCACCGAG CCGGCTGCCC CGTAGAGACG 
GGGAGGACCG CAGAGAGCTG CTGCAAGGTC AGCCAGTCCT CCTGCTCCTC CAGCTTCATC 
AGCAGGCCCG GGGTGGACCC CGCGGGCTCC CTTAGCGACT CCCTCTACGA CAGTTTCTCC 
TCCTGCACCA GTCAGGGCTC CAACGATGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101162908 ( XM_020708323.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020563982.1 Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X1, mRNA.

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CloneID	OJa74623
Clone ID Related Accession (Same CDS sequence)	XM_020708323.1 , XM_020708323.2
Accession Version	XM_020708323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020708323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGT TTACAGATCA CAGTGTCGCG TCGGTCTGCA GGAGGATCCG AAACATTCCC 
CTGCTGGAGC TGCTGGCGGA GTACATGGAC GCCAACAAAT GCATCGATGA GCTTCTGAAA 
CAGCTTGAGG AGGAGCGCCG GAATGTCCGA AGGGAAAAGC TGGCTGTGGC TCGCCTGCAG 
AGGGAAGTCG CACGAAGCAA GAGTGAAGGA ACAATGCGAG AAAAGTTGAT CCACGAGCTA 
GAGGAAGAAC GACGCCTGCG ACTGGAGAGT GAGAAGCGTC TCCGGGAAGT GACGGAGGAG 
TCAGAGCTGG GAAGGGCGCA GATGATTTCA CTGCAGCAGC AGTTCTCCAG GATGGAAGAG 
ACGGTGCGGT CGCTGCTGCA GAACCAGGGC GTCCTGGAGC AGACTGCTGT GGACACAGTG 
GACATCATGA AGGCCTACAA GGATAAACTC TCCGAGGAAG TGCAGAAACT GCAGGATGGC 
CCAGAGGCGA AGCAGGCCGA GCCAGCGAGT ACCGAGCTCG ACGGCAGCCA AGCGGACGAA 
GACAGAGACA AAACCAAGCT CCTTCTGGAG CGCCTGAAGA CCCTCGAGGA GGAGAACTCG 
GCGTTGGCCT CGGAGAACGA GAGCCAGAGG GAACAGTACG AGCGTTGCCT GGACGAGGTC 
GCTAACCAAG TTGTACAGGC ACTCCTCACA CAGAAGGATC TGAGAGAGGA ATGTCTTAAG 
CTGCGGACAA GAGTTTTTGA TCTGGAGCAG CAGAATCGAG CACTGAGTGT TTTGTTCCAG 
CAGAAGATAA AGCCGGCATC AGACCTCCTC CTTCAGAAAC TCCACTCGCG GATCATGGAT 
CTGTCTGCGG CAGATTTGCT TCTGGAGCCG GAGAGGAGCA AGGCCTTCCT CCTTTCCAGG 
AATACAGACT CCCCTTCCAA CGAGGTTCAT TCGAATGGAA AGCCAGGTGT CCCTGTGGCC 
AAATGTCTGA GCCAGATGAG TCTGATGGCG CCCGCTCCCA TCTATTCTCG CAGCAGCAGC 
GAGCTCTCAC TGTCGAGCGC GTGCAGCGAG TTCTCCAGCG GCTCCTACAC CTGGAATGAT 
GGGCGATCCT GCGGGAAAAT GTCATCCCTG ACGTGGGAGA AGAGACTCAG TTTGGGCTCG 
TCAGCTCCAA GTAACATCTG CGCGCCGCTG GAGGAACAGC CCCCCACACG GCGTAAGGAG 
AGCCACATAC TGGAGGGCCT GAGAAAACTC CAGCGGAGGA AACACAGGAG CTCCTCCTGC 
TCCTCTAGAG TGTCCAAGTC AGGCTACAAA GATTGCATGA ACTCCAACGA GGGCATCTAC 
TCTTTGGGTA TCAAGAGCAG CAGCAAAGGG GTGTCTAAAC CTGTACATGT CAGCAGAGCT 
TCTGCTGCTC TTGGAAGGCG GTTTTCCTAC GATTCCGATG ATGCTGACGA TGAACTGGCA 
CGCTCTAGTT GCAGAGATAA TATTCCCACT AAGGAGAGCT GGATTCACTG CAAGAAGCTC 
TCCCACAGCA TCTCTGATAG TTTGTGCAGC TTGGAGGGGA CACAGGAGGC TGTGGGTGAT 
GGTACTTCTT GTCCCACAGC TTCAAAACCC TATGACTCAA GGGAGCGTCC GGAGAAGCTC 
ATGAGTTTCA TCAACAGTTT TCTTCCTTTA GAAGGGCGTA AATCAGCCTT TAGCAGGCCA 
ACCGTGCACC ACAGAAATCC TACTCAAGTA GAAGATCCGA GTTGCAGATC CAACGCAGAT 
GATCCAGAGG AAACCATTCC TGATTCTGTG GGGGTCAAGA CGCCATGCAG TCAGCCGCAA 
GAACTAGCAG AGAGAGATAT CAAGAGGCTC TCAAGGGATA CCATCAAGAT TTTCAAGCAA 
CAGTTCATGC AAAAAGATCA GGGGCGCACT CAGTCTGCGG ATGGGAGACC GAAGCCTTTC 
AGCCTATTGA AGGAACTTAA AGCAACCAAG TGCACCCAGT CTGAGGAGAG CATACTGGCG 
ATATTTGATG CTGAAGGAGA ACCTATTGAT CTGTGCGCTC AGAGACTAGT ATCAGGAGCA 
GAAGGTCGCA AAGCCGCTAG CTGTTGCTCA GAGTTCCAAA AGAGGACAAC AAGGCAAAAT 
TCAGCAAACA CGACAAACTA TAGCGTTCTT GAATCTCCAG AAAAACCTTC AGAACATCAG 
ACTAGATCAG GCAAAGGCAG TGATAGCATG GAAGGAAGTG CAGAAAGGCT ACAGTTAGCT 
CCTCAGAGGA AGTTGATAAA GCCACCAAGC AGTCGAACTA ATAAAGGCTT TTCCATTCCT 
CCCATGAGTG ACGTCAACAG CCCCAAATCT GGTGGCTCTA AGATCCCCGG TGTCAATAAG 
CCCTTAGCTT CTCCGTTAAG AGTATCCAAG GGCACAACCA CTGAGCCTAA TAACAGTGGA 
AACTCAGGAC CCTCTGCTCA GGAGAAATCT TCGTCTTCTC TTACGGTTAA GATGTCTAGA 
TTCATCAAGA CGCAGGGAAC TTCCACTCAG AGTCCGAAAG CTGTGAACTG CAAGGTGTCT 
GGTCAGGCCG ACTTAAGCAA GACCTCTTCT TCCACTTCCC CCCACCTTCC AAAAAGACGC 
CTCGACTTCT CTGACAACAG CGAACAGCCG ACCAGAGACA AACACTGTGA AACCAGCAAG 
AACAAACTCA GGTCTCCATC TCCTCCCCCT CCCCCAGGAC GCACCACCTC TTTACTGGTC 
AGACCAAATT ACGAAGCGTC GCCTCAAGCA CACAAATCAG GGGCTCAACC ATCCACGCCA 
ACCACTGTGA GGGGCCCTCC CCCAAGTTAC CACACCTCAG TTGCACCAAA TATGCAAGGC 
ACGCTCCCCA TCAAGGATAA AGACTGTTTA GACGTAGATG CAGGCTATGG GACTGCACTT 
GCACCTCAGA AGCTGGTGGA GAAAACCACC CAGCACTTTC AGAAGACCAC TGCCGTAACT 
CAGACTCCAA CTAAAGGCAC TTCCAAGCGA ATAAAAGACT ACCTCCCACC TGCAAGCTCA 
GGATGTGCCA GAGTACCTGA AATTGCACCT AAAAACTCGA AGAATGTCCC TCCTCCCTAC 
AGTGCTCTAA GAGTGCCCTC CCTTCAAAAC TCCTTTGCAA ACAAAAAGGT ACCTGCTCAA 
GAAAACACCC ATCAGTCAGT GCAAAAGACT TCCATTAGTT TACCAATATC GCTTCAGGAT 
TCTGCTCAGT GTCAAACAGA GCCACAAAAA GCTGTCATGA GCCCTCCGGG CTCAGCTGTT 
ACGTCACCCA ATTTGTCAGA AAAAGCCTCA AAGACTCGAA TCCCAATGGG ATTTAAAGCC 
TTTCTAAAAT CCCCTCCCAG CCATAAAAGT AGTCCTACAA TAGCAGGCAA GCAAGAGAAA 
GATCATATCA ACTCTGTCTC CAAGGAAACT GTGACTTCAA ATGCTTCTAC TCAGTGTGAC 
ACCTCGCAGG CAGCGTACAG TACGGATTCA ACACCTACCG TGTCTGTTCC CGAAGGGAAA 
GGTGAATTTC TGTGTCAGCC ACAGAAAGAG GACTTACAAC CCCCCTTGTC GTCAGCAGAA 
GGAAATGTGA GTGACAAGGC AAAAAGGAGT AGTCAACTCT TTGGCAGATC CATATCTATT 
AATACCAAAC CACACCTAAA GCCAGCTTTA GGGATGAACG GGGCCAAAGC TCGAAGCCAG 
AGCTTCAGCA CCAACTACAT AGAGAAACCC AACATCAATG CCATGGAGGG GCCGGGAAGG 
ATCAGGACTC AAATCATCAC TAACTCAGGT GAAAGGGGGA ATTCGCTGTC AAGGCAGAGT 
TCTTCAGAAA CAACTGGGGT TGGATTGGCT GAAAGTCCAA TCCATGCCCC TAGAACCCGG 
CTAAGCCATC ATGGTGGCAT GATGGGACCA AATAGTCAGA GCGGCCTCCC TGAGAAGTCC 
ATTCAGTCGT GTGTTATAGG TGAAGGAAAA GCTTGTGGAG TGAAAGGAGA TGTGGTCACC 
TCTCCTTCTC AAAGAGAAGC TCGCAGCTTG CCCAAAAGTG ACAGAACTGC CTTTATTAAT 
GTCCAAAAGC CGGCAAAAGT GGACTCCCAT CCTCAAATCC AGTCTTCCTC AGCTTTTGTC 
TTTAGTCCTG AAAATCCTAC CAGAGAAGCA GAAACCATTA CCACCACTCC TGCTGAGCCA 
GACAAAACCC AGTTGGGTTC CCCAGGCAAT TTCCCAGACA CAGAGGAGAA AAAAATCAGC 
CCAACAACGT GCACTATTGA AGAGAAAGTG ATGATGGGGA TAGAAGAAAA CCTGCAGAAA 
AGTCAAGAGC AGGAGAAGGC TGCAGCTAAT GAGGCCAAAC AGAAGACGGG CCCGTCTTTG 
GCCAACTGGT TTGGCCTCCG CAAGAGCAAA CTACCAGCTT TTAGCGGCAA AAAAGCTGAT 
TCCCCGAAGG CAAGAGACGA GAAGAAAGAG CTGAAGATTG GGTCTGTGAT CAGCGGCAAG 
CAGATGAAGT CTGATAAGAA GAAGGAAAGG AAGAAGGATG GTCAGGAGGT CCAGAATTTG 
TCAGAGATGA ACAACAAGCT GAGCTCCATC ATGGACCACT GTAACAATCA GATGGGTCAG 
ATCGCCAGTC AGATCCAGAG CACCACTGTG TTAATCGGCA AGGACCAGTT TGTAAAAGAG 
CTTCTTGGGA GAGCTACTGT GAAGGGCAAC GCTTCGCCAC CTGGCATCTC TACACCGAAG 
AAACACTGTG AAACAAAGGG CGACATGCAG ATCTGTCCAG ATAATACCCT CATACTGACG 
CAAAAGATCA GCCTCAAGGC CAAAAACGAC GAGGGGAACA TTCCGGACAC GTCTTGTCAA 
GACCACATGA TAGGCTCCAG CTGTCAGATG AGAACCTTGG ACAGCGGCAT TGGAACCTTC 
CCCCTGCCCG ATTCGGTCAC GCGGGCCAGC AGTCGCCACA TCCCCAAATC TGAGTCCAGT 
CCAGATGGGG TGATGGCCGG CTCAACTGAG CGCAGTCGGG ACACTCCCTC CTCCCACCCT 
GACCCCTCAC AGTCAGATTT CAAAGTGCCT TCCCCTTCAA AAGCCCATGT GCACACCCCC 
ACTACCAGCA TGGGTCACTC CCTGTCTGAT CCCACTGTGA CATGCAGCAG GAATGCCCTG 
GAGACCCAGA GTCGCCTGCC CAAGCTGGCA TCCACAGACG CAATCAAGAC CAAAAGGTTG 
AGTCTTTGTG CCCAGCGCAG CGCCGTCTCC GCTTCTGCCG AGGGAGACGG GAAAGAAATG 
GAGAGGAAGA TGAAAACAAG CGAGCATGAA TCACCTGGTG AGACAACATT GCGGGTTTGC 
GTGTATCCAG GCAGCGACAG TGACACGGAG ACTGAATCCG AGGGAACTAC AAGCACTTTG 
GCGTCGCCGC TAAGAACCCC CATGGACAGA CCTCAGAAGA ACGACTCTGT CGAGCAGAAT 
GAGGAGACCT TGAAAAAACA GAATGCAGAG AAATCCCTGT CCATTATGGA CTACTATCAG 
CACGATGTGT TCTTGCACCT GGAGACAGAC GCCAGGAGGA TTTCCCAGTA CACCTTGCTG 
CACAAAGAGT CGTCCCTCGA CGGAGAAGCA GGAGACAGGC TCAGCAAAGA GATTCCTGTC 
AGTCCCAACC AGCCGGCCTC TTTGGACGTC TCCTTGGAAT CGTTGAACAA AATGAGCCAC 
AGCAGCTCCA GCTGCCTGTG CGCGGACGCC AGCCACCGAG CCGGCTGCCC CGTAGAGACG 
GGGAGGACCG CAGAGAGCTG CTGCAAGGTC AGCCAGTCCT CCTGCTCCTC CAGCTTCATC 
AGCAGGCCCG GGGTGGACCC CGCGGGCTCC CTTAGCGACT CCCTCTACGA CAGTTTCTCC 
TCCTGCACCA GTCAGGGCTC CAACGATGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020563982.1
CDS	130..5982
Translation	MSQFTDHSVASVCRRIRNIPLLELLAEYMDANKCIDELLKQLEEERRNVRREKLAVARLQREVARSKSEGTMREKLIHELEEERRLRLESEKRLREVTEESELGRAQMISLQQQFSRMEETVRSLLQNQGVLEQTAVDTVDIMKAYKDKLSEEVQKLQDGPEAKQAEPASTELDGSQADEDRDKTKLLLERLKTLEEENSALASENESQREQYERCLDEVANQVVQALLTQKDLREECLKLRTRVFDLEQQNRALSVLFQQKIKPASDLLLQKLHSRIMDLSAADLLLEPERSKAFLLSRNTDSPSNEVHSNGKPGVPVAKCLSQMSLMAPAPIYSRSSSELSLSSACSEFSSGSYTWNDGRSCGKMSSLTWEKRLSLGSSAPSNICAPLEEQPPTRRKESHILEGLRKLQRRKHRSSSCSSRVSKSGYKDCMNSNEGIYSLGIKSSSKGVSKPVHVSRASAALGRRFSYDSDDADDELARSSCRDNIPTKESWIHCKKLSHSISDSLCSLEGTQEAVGDGTSCPTASKPYDSRERPEKLMSFINSFLPLEGRKSAFSRPTVHHRNPTQVEDPSCRSNADDPEETIPDSVGVKTPCSQPQELAERDIKRLSRDTIKIFKQQFMQKDQGRTQSADGRPKPFSLLKELKATKCTQSEESILAIFDAEGEPIDLCAQRLVSGAEGRKAASCCSEFQKRTTRQNSANTTNYSVLESPEKPSEHQTRSGKGSDSMEGSAERLQLAPQRKLIKPPSSRTNKGFSIPPMSDVNSPKSGGSKIPGVNKPLASPLRVSKGTTTEPNNSGNSGPSAQEKSSSSLTVKMSRFIKTQGTSTQSPKAVNCKVSGQADLSKTSSSTSPHLPKRRLDFSDNSEQPTRDKHCETSKNKLRSPSPPPPPGRTTSLLVRPNYEASPQAHKSGAQPSTPTTVRGPPPSYHTSVAPNMQGTLPIKDKDCLDVDAGYGTALAPQKLVEKTTQHFQKTTAVTQTPTKGTSKRIKDYLPPASSGCARVPEIAPKNSKNVPPPYSALRVPSLQNSFANKKVPAQENTHQSVQKTSISLPISLQDSAQCQTEPQKAVMSPPGSAVTSPNLSEKASKTRIPMGFKAFLKSPPSHKSSPTIAGKQEKDHINSVSKETVTSNASTQCDTSQAAYSTDSTPTVSVPEGKGEFLCQPQKEDLQPPLSSAEGNVSDKAKRSSQLFGRSISINTKPHLKPALGMNGAKARSQSFSTNYIEKPNINAMEGPGRIRTQIITNSGERGNSLSRQSSSETTGVGLAESPIHAPRTRLSHHGGMMGPNSQSGLPEKSIQSCVIGEGKACGVKGDVVTSPSQREARSLPKSDRTAFINVQKPAKVDSHPQIQSSSAFVFSPENPTREAETITTTPAEPDKTQLGSPGNFPDTEEKKISPTTCTIEEKVMMGIEENLQKSQEQEKAAANEAKQKTGPSLANWFGLRKSKLPAFSGKKADSPKARDEKKELKIGSVISGKQMKSDKKKERKKDGQEVQNLSEMNNKLSSIMDHCNNQMGQIASQIQSTTVLIGKDQFVKELLGRATVKGNASPPGISTPKKHCETKGDMQICPDNTLILTQKISLKAKNDEGNIPDTSCQDHMIGSSCQMRTLDSGIGTFPLPDSVTRASSRHIPKSESSPDGVMAGSTERSRDTPSSHPDPSQSDFKVPSPSKAHVHTPTTSMGHSLSDPTVTCSRNALETQSRLPKLASTDAIKTKRLSLCAQRSAVSASAEGDGKEMERKMKTSEHESPGETTLRVCVYPGSDSDTETESEGTTSTLASPLRTPMDRPQKNDSVEQNEETLKKQNAEKSLSIMDYYQHDVFLHLETDARRISQYTLLHKESSLDGEAGDRLSKEIPVSPNQPASLDVSLESLNKMSHSSSSCLCADASHRAGCPVETGRTAESCCKVSQSSCSSSFISRPGVDPAGSLSDSLYDSFSSCTSQGSNDV

Target ORF information:

RefSeq Version	XM_020708323.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020708323.2

ORF Insert Sequence:

ATGAGCCAGT TTACAGATCA CAGTGTCGCG TCGGTCTGCA GGAGGATCCG AAACATTCCC 
CTGCTGGAGC TGCTGGCGGA GTACATGGAC GCCAACAAAT GCATCGATGA GCTTCTGAAA 
CAGCTTGAGG AGGAGCGCCG GAATGTCCGA AGGGAAAAGC TGGCTGTGGC TCGCCTGCAG 
AGGGAAGTCG CACGAAGCAA GAGTGAAGGA ACAATGCGAG AAAAGTTGAT CCACGAGCTA 
GAGGAAGAAC GACGCCTGCG ACTGGAGAGT GAGAAGCGTC TCCGGGAAGT GACGGAGGAG 
TCAGAGCTGG GAAGGGCGCA GATGATTTCA CTGCAGCAGC AGTTCTCCAG GATGGAAGAG 
ACGGTGCGGT CGCTGCTGCA GAACCAGGGC GTCCTGGAGC AGACTGCTGT GGACACAGTG 
GACATCATGA AGGCCTACAA GGATAAACTC TCCGAGGAAG TGCAGAAACT GCAGGATGGC 
CCAGAGGCGA AGCAGGCCGA GCCAGCGAGT ACCGAGCTCG ACGGCAGCCA AGCGGACGAA 
GACAGAGACA AAACCAAGCT CCTTCTGGAG CGCCTGAAGA CCCTCGAGGA GGAGAACTCG 
GCGTTGGCCT CGGAGAACGA GAGCCAGAGG GAACAGTACG AGCGTTGCCT GGACGAGGTC 
GCTAACCAAG TTGTACAGGC ACTCCTCACA CAGAAGGATC TGAGAGAGGA ATGTCTTAAG 
CTGCGGACAA GAGTTTTTGA TCTGGAGCAG CAGAATCGAG CACTGAGTGT TTTGTTCCAG 
CAGAAGATAA AGCCGGCATC AGACCTCCTC CTTCAGAAAC TCCACTCGCG GATCATGGAT 
CTGTCTGCGG CAGATTTGCT TCTGGAGCCG GAGAGGAGCA AGGCCTTCCT CCTTTCCAGG 
AATACAGACT CCCCTTCCAA CGAGGTTCAT TCGAATGGAA AGCCAGGTGT CCCTGTGGCC 
AAATGTCTGA GCCAGATGAG TCTGATGGCG CCCGCTCCCA TCTATTCTCG CAGCAGCAGC 
GAGCTCTCAC TGTCGAGCGC GTGCAGCGAG TTCTCCAGCG GCTCCTACAC CTGGAATGAT 
GGGCGATCCT GCGGGAAAAT GTCATCCCTG ACGTGGGAGA AGAGACTCAG TTTGGGCTCG 
TCAGCTCCAA GTAACATCTG CGCGCCGCTG GAGGAACAGC CCCCCACACG GCGTAAGGAG 
AGCCACATAC TGGAGGGCCT GAGAAAACTC CAGCGGAGGA AACACAGGAG CTCCTCCTGC 
TCCTCTAGAG TGTCCAAGTC AGGCTACAAA GATTGCATGA ACTCCAACGA GGGCATCTAC 
TCTTTGGGTA TCAAGAGCAG CAGCAAAGGG GTGTCTAAAC CTGTACATGT CAGCAGAGCT 
TCTGCTGCTC TTGGAAGGCG GTTTTCCTAC GATTCCGATG ATGCTGACGA TGAACTGGCA 
CGCTCTAGTT GCAGAGATAA TATTCCCACT AAGGAGAGCT GGATTCACTG CAAGAAGCTC 
TCCCACAGCA TCTCTGATAG TTTGTGCAGC TTGGAGGGGA CACAGGAGGC TGTGGGTGAT 
GGTACTTCTT GTCCCACAGC TTCAAAACCC TATGACTCAA GGGAGCGTCC GGAGAAGCTC 
ATGAGTTTCA TCAACAGTTT TCTTCCTTTA GAAGGGCGTA AATCAGCCTT TAGCAGGCCA 
ACCGTGCACC ACAGAAATCC TACTCAAGTA GAAGATCCGA GTTGCAGATC CAACGCAGAT 
GATCCAGAGG AAACCATTCC TGATTCTGTG GGGGTCAAGA CGCCATGCAG TCAGCCGCAA 
GAACTAGCAG AGAGAGATAT CAAGAGGCTC TCAAGGGATA CCATCAAGAT TTTCAAGCAA 
CAGTTCATGC AAAAAGATCA GGGGCGCACT CAGTCTGCGG ATGGGAGACC GAAGCCTTTC 
AGCCTATTGA AGGAACTTAA AGCAACCAAG TGCACCCAGT CTGAGGAGAG CATACTGGCG 
ATATTTGATG CTGAAGGAGA ACCTATTGAT CTGTGCGCTC AGAGACTAGT ATCAGGAGCA 
GAAGGTCGCA AAGCCGCTAG CTGTTGCTCA GAGTTCCAAA AGAGGACAAC AAGGCAAAAT 
TCAGCAAACA CGACAAACTA TAGCGTTCTT GAATCTCCAG AAAAACCTTC AGAACATCAG 
ACTAGATCAG GCAAAGGCAG TGATAGCATG GAAGGAAGTG CAGAAAGGCT ACAGTTAGCT 
CCTCAGAGGA AGTTGATAAA GCCACCAAGC AGTCGAACTA ATAAAGGCTT TTCCATTCCT 
CCCATGAGTG ACGTCAACAG CCCCAAATCT GGTGGCTCTA AGATCCCCGG TGTCAATAAG 
CCCTTAGCTT CTCCGTTAAG AGTATCCAAG GGCACAACCA CTGAGCCTAA TAACAGTGGA 
AACTCAGGAC CCTCTGCTCA GGAGAAATCT TCGTCTTCTC TTACGGTTAA GATGTCTAGA 
TTCATCAAGA CGCAGGGAAC TTCCACTCAG AGTCCGAAAG CTGTGAACTG CAAGGTGTCT 
GGTCAGGCCG ACTTAAGCAA GACCTCTTCT TCCACTTCCC CCCACCTTCC AAAAAGACGC 
CTCGACTTCT CTGACAACAG CGAACAGCCG ACCAGAGACA AACACTGTGA AACCAGCAAG 
AACAAACTCA GGTCTCCATC TCCTCCCCCT CCCCCAGGAC GCACCACCTC TTTACTGGTC 
AGACCAAATT ACGAAGCGTC GCCTCAAGCA CACAAATCAG GGGCTCAACC ATCCACGCCA 
ACCACTGTGA GGGGCCCTCC CCCAAGTTAC CACACCTCAG TTGCACCAAA TATGCAAGGC 
ACGCTCCCCA TCAAGGATAA AGACTGTTTA GACGTAGATG CAGGCTATGG GACTGCACTT 
GCACCTCAGA AGCTGGTGGA GAAAACCACC CAGCACTTTC AGAAGACCAC TGCCGTAACT 
CAGACTCCAA CTAAAGGCAC TTCCAAGCGA ATAAAAGACT ACCTCCCACC TGCAAGCTCA 
GGATGTGCCA GAGTACCTGA AATTGCACCT AAAAACTCGA AGAATGTCCC TCCTCCCTAC 
AGTGCTCTAA GAGTGCCCTC CCTTCAAAAC TCCTTTGCAA ACAAAAAGGT ACCTGCTCAA 
GAAAACACCC ATCAGTCAGT GCAAAAGACT TCCATTAGTT TACCAATATC GCTTCAGGAT 
TCTGCTCAGT GTCAAACAGA GCCACAAAAA GCTGTCATGA GCCCTCCGGG CTCAGCTGTT 
ACGTCACCCA ATTTGTCAGA AAAAGCCTCA AAGACTCGAA TCCCAATGGG ATTTAAAGCC 
TTTCTAAAAT CCCCTCCCAG CCATAAAAGT AGTCCTACAA TAGCAGGCAA GCAAGAGAAA 
GATCATATCA ACTCTGTCTC CAAGGAAACT GTGACTTCAA ATGCTTCTAC TCAGTGTGAC 
ACCTCGCAGG CAGCGTACAG TACGGATTCA ACACCTACCG TGTCTGTTCC CGAAGGGAAA 
GGTGAATTTC TGTGTCAGCC ACAGAAAGAG GACTTACAAC CCCCCTTGTC GTCAGCAGAA 
GGAAATGTGA GTGACAAGGC AAAAAGGAGT AGTCAACTCT TTGGCAGATC CATATCTATT 
AATACCAAAC CACACCTAAA GCCAGCTTTA GGGATGAACG GGGCCAAAGC TCGAAGCCAG 
AGCTTCAGCA CCAACTACAT AGAGAAACCC AACATCAATG CCATGGAGGG GCCGGGAAGG 
ATCAGGACTC AAATCATCAC TAACTCAGGT GAAAGGGGGA ATTCGCTGTC AAGGCAGAGT 
TCTTCAGAAA CAACTGGGGT TGGATTGGCT GAAAGTCCAA TCCATGCCCC TAGAACCCGG 
CTAAGCCATC ATGGTGGCAT GATGGGACCA AATAGTCAGA GCGGCCTCCC TGAGAAGTCC 
ATTCAGTCGT GTGTTATAGG TGAAGGAAAA GCTTGTGGAG TGAAAGGAGA TGTGGTCACC 
TCTCCTTCTC AAAGAGAAGC TCGCAGCTTG CCCAAAAGTG ACAGAACTGC CTTTATTAAT 
GTCCAAAAGC CGGCAAAAGT GGACTCCCAT CCTCAAATCC AGTCTTCCTC AGCTTTTGTC 
TTTAGTCCTG AAAATCCTAC CAGAGAAGCA GAAACCATTA CCACCACTCC TGCTGAGCCA 
GACAAAACCC AGTTGGGTTC CCCAGGCAAT TTCCCAGACA CAGAGGAGAA AAAAATCAGC 
CCAACAACGT GCACTATTGA AGAGAAAGTG ATGATGGGGA TAGAAGAAAA CCTGCAGAAA 
AGTCAAGAGC AGGAGAAGGC TGCAGCTAAT GAGGCCAAAC AGAAGACGGG CCCGTCTTTG 
GCCAACTGGT TTGGCCTCCG CAAGAGCAAA CTACCAGCTT TTAGCGGCAA AAAAGCTGAT 
TCCCCGAAGG CAAGAGACGA GAAGAAAGAG CTGAAGATTG GGTCTGTGAT CAGCGGCAAG 
CAGATGAAGT CTGATAAGAA GAAGGAAAGG AAGAAGGATG GTCAGGAGGT CCAGAATTTG 
TCAGAGATGA ACAACAAGCT GAGCTCCATC ATGGACCACT GTAACAATCA GATGGGTCAG 
ATCGCCAGTC AGATCCAGAG CACCACTGTG TTAATCGGCA AGGACCAGTT TGTAAAAGAG 
CTTCTTGGGA GAGCTACTGT GAAGGGCAAC GCTTCGCCAC CTGGCATCTC TACACCGAAG 
AAACACTGTG AAACAAAGGG CGACATGCAG ATCTGTCCAG ATAATACCCT CATACTGACG 
CAAAAGATCA GCCTCAAGGC CAAAAACGAC GAGGGGAACA TTCCGGACAC GTCTTGTCAA 
GACCACATGA TAGGCTCCAG CTGTCAGATG AGAACCTTGG ACAGCGGCAT TGGAACCTTC 
CCCCTGCCCG ATTCGGTCAC GCGGGCCAGC AGTCGCCACA TCCCCAAATC TGAGTCCAGT 
CCAGATGGGG TGATGGCCGG CTCAACTGAG CGCAGTCGGG ACACTCCCTC CTCCCACCCT 
GACCCCTCAC AGTCAGATTT CAAAGTGCCT TCCCCTTCAA AAGCCCATGT GCACACCCCC 
ACTACCAGCA TGGGTCACTC CCTGTCTGAT CCCACTGTGA CATGCAGCAG GAATGCCCTG 
GAGACCCAGA GTCGCCTGCC CAAGCTGGCA TCCACAGACG CAATCAAGAC CAAAAGGTTG 
AGTCTTTGTG CCCAGCGCAG CGCCGTCTCC GCTTCTGCCG AGGGAGACGG GAAAGAAATG 
GAGAGGAAGA TGAAAACAAG CGAGCATGAA TCACCTGGTG AGACAACATT GCGGGTTTGC 
GTGTATCCAG GCAGCGACAG TGACACGGAG ACTGAATCCG AGGGAACTAC AAGCACTTTG 
GCGTCGCCGC TAAGAACCCC CATGGACAGA CCTCAGAAGA ACGACTCTGT CGAGCAGAAT 
GAGGAGACCT TGAAAAAACA GAATGCAGAG AAATCCCTGT CCATTATGGA CTACTATCAG 
CACGATGTGT TCTTGCACCT GGAGACAGAC GCCAGGAGGA TTTCCCAGTA CACCTTGCTG 
CACAAAGAGT CGTCCCTCGA CGGAGAAGCA GGAGACAGGC TCAGCAAAGA GATTCCTGTC 
AGTCCCAACC AGCCGGCCTC TTTGGACGTC TCCTTGGAAT CGTTGAACAA AATGAGCCAC 
AGCAGCTCCA GCTGCCTGTG CGCGGACGCC AGCCACCGAG CCGGCTGCCC CGTAGAGACG 
GGGAGGACCG CAGAGAGCTG CTGCAAGGTC AGCCAGTCCT CCTGCTCCTC CAGCTTCATC 
AGCAGGCCCG GGGTGGACCC CGCGGGCTCC CTTAGCGACT CCCTCTACGA CAGTTTCTCC 
TCCTGCACCA GTCAGGGCTC CAACGATGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101162908 ( XM_020708327.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020563986.1 Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OJa74624
Clone ID Related Accession (Same CDS sequence)	XM_020708327.1 , XM_020708327.2
Accession Version	XM_020708327.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	nck-associated protein 5-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG ATGCTGCATC TCCATCAGGG GAAAAGCTGG CTGTGGCTCG CCTGCAGAGG 
GAAGTCGCAC GAAGCAAGAG TGAAGGAACA ATGCGAGAAA AGTTGATCCA CGAGCTAGAG 
GAAGAACGAC GCCTGCGACT GGAGAGTGAG AAGCGTCTCC GGGAAGTGAC GGAGGAGTCA 
GAGCTGGGAA GGGCGCAGAT GATTTCACTG CAGCAGCAGT TCTCCAGGAT GGAAGAGACG 
GTGCGGTCGC TGCTGCAGAA CCAGGGCGTC CTGGAGCAGA CTGCTGTGGA CACAGTGGAC 
ATCATGAAGG CCTACAAGGA TAAACTCTCC GAGGAAGTGC AGAAACTGCA GGATGGCCCA 
GAGGCGAAGC AGGCCGAGCC AGCGAGTACC GAGCTCGACG GCAGCCAAGC GGACGAAGAC 
AGAGACAAAA CCAAGCTCCT TCTGGAGCGC CTGAAGACCC TCGAGGAGGA GAACTCGGCG 
TTGGCCTCGG AGAACGAGAG CCAGAGGGAA CAGTACGAGC GTTGCCTGGA CGAGGTCGCT 
AACCAAGTTG TACAGGCACT CCTCACACAG AAGGATCTGA GAGAGGAATG TCTTAAGCTG 
CGGACAAGAG TTTTTGATCT GGAGCAGCAG AATCGAGCAC TGAGTGTTTT GTTCCAGCAG 
AAGATAAAGC CGGCATCAGA CCTCCTCCTT CAGAAACTCC ACTCGCGGAT CATGGATCTG 
TCTGCGGCAG ATTTGCTTCT GGAGCCGGAG AGGAGCAAGG CCTTCCTCCT TTCCAGGAAT 
ACAGACTCCC CTTCCAACGA GGTTCATTCG AATGGAAAGC CAGGTGTCCC TGTGGCCAAA 
TGTCTGAGCC AGATGAGTCT GATGGCGCCC GCTCCCATCT ATTCTCGCAG CAGCAGCGAG 
CTCTCACTGT CGAGCGCGTG CAGCGAGTTC TCCAGCGGCT CCTACACCTG GAATGATGGG 
CGATCCTGCG GGAAAATGTC ATCCCTGACG TGGGAGAAGA GACTCAGTTT GGGCTCGTCA 
GCTCCAAGTA ACATCTGCGC GCCGCTGGAG GAACAGCCCC CCACACGGCG TAAGGAGAGC 
CACATACTGG AGGGCCTGAG AAAACTCCAG CGGAGGAAAC ACAGGAGCTC CTCCTGCTCC 
TCTAGAGTGT CCAAGTCAGG CTACAAAGAT TGCATGAACT CCAACGAGGG CATCTACTCT 
TTGGGTATCA AGAGCAGCAG CAAAGGGGTG TCTAAACCTG TACATGTCAG CAGAGCTTCT 
GCTGCTCTTG GAAGGCGGTT TTCCTACGAT TCCGATGATG CTGACGATGA ACTGGCACGC 
TCTAGTTGCA GAGATAATAT TCCCACTAAG GAGAGCTGGA TTCACTGCAA GAAGCTCTCC 
CACAGCATCT CTGATAGTTT GTGCAGCTTG GAGGGGACAC AGGAGGCTGT GGGTGATGGT 
ACTTCTTGTC CCACAGCTTC AAAACCCTAT GACTCAAGGG AGCGTCCGGA GAAGCTCATG 
AGTTTCATCA ACAGTTTTCT TCCTTTAGAA GGGCGTAAAT CAGCCTTTAG CAGGCCAACC 
GTGCACCACA GAAATCCTAC TCAAGTAGAA GATCCGAGTT GCAGATCCAA CGCAGATGAT 
CCAGAGGAAA CCATTCCTGA TTCTGTGGGG GTCAAGACGC CATGCAGTCA GCCGCAAGAA 
CTAGCAGAGA GAGATATCAA GAGGCTCTCA AGGGATACCA TCAAGATTTT CAAGCAACAG 
TTCATGCAAA AAGATCAGGG GCGCACTCAG TCTGCGGATG GGAGACCGAA GCCTTTCAGC 
CTATTGAAGG AACTTAAAGC AACCAAGTGC ACCCAGTCTG AGGAGAGCAT ACTGGCGATA 
TTTGATGCTG AAGGAGAACC TATTGATCTG TGCGCTCAGA GACTAGTATC AGGAGCAGAA 
GGTCGCAAAG CCGCTAGCTG TTGCTCAGAG TTCCAAAAGA GGACAACAAG GCAAAATTCA 
GCAAACACGA CAAACTATAG CGTTCTTGAA TCTCCAGAAA AACCTTCAGA ACATCAGACT 
AGATCAGGCA AAGGCAGTGA TAGCATGGAA GGAAGTGCAG AAAGGCTACA GTTAGCTCCT 
CAGAGGAAGT TGATAAAGCC ACCAAGCAGT CGAACTAATA AAGGCTTTTC CATTCCTCCC 
ATGAGTGACG TCAACAGCCC CAAATCTGGT GGCTCTAAGA TCCCCGGTGT CAATAAGCCC 
TTAGCTTCTC CGTTAAGAGT ATCCAAGGGC ACAACCACTG AGCCTAATAA CAGTGGAAAC 
TCAGGACCCT CTGCTCAGGA GAAATCTTCG TCTTCTCTTA CGGTTAAGAT GTCTAGATTC 
ATCAAGACGC AGGGAACTTC CACTCAGAGT CCGAAAGCTG TGAACTGCAA GGTGTCTGGT 
CAGGCCGACT TAAGCAAGAC CTCTTCTTCC ACTTCCCCCC ACCTTCCAAA AAGACGCCTC 
GACTTCTCTG ACAACAGCGA ACAGCCGACC AGAGACAAAC ACTGTGAAAC CAGCAAGAAC 
AAACTCAGGT CTCCATCTCC TCCCCCTCCC CCAGGACGCA CCACCTCTTT ACTGGTCAGA 
CCAAATTACG AAGCGTCGCC TCAAGCACAC AAATCAGGGG CTCAACCATC CACGCCAACC 
ACTGTGAGGG GCCCTCCCCC AAGTTACCAC ACCTCAGTTG CACCAAATAT GCAAGGCACG 
CTCCCCATCA AGGATAAAGA CTGTTTAGAC GTAGATGCAG GCTATGGGAC TGCACTTGCA 
CCTCAGAAGC TGGTGGAGAA AACCACCCAG CACTTTCAGA AGACCACTGC CGTAACTCAG 
ACTCCAACTA AAGGCACTTC CAAGCGAATA AAAGACTACC TCCCACCTGC AAGCTCAGGA 
TGTGCCAGAG TACCTGAAAT TGCACCTAAA AACTCGAAGA ATGTCCCTCC TCCCTACAGT 
GCTCTAAGAG TGCCCTCCCT TCAAAACTCC TTTGCAAACA AAAAGGTACC TGCTCAAGAA 
AACACCCATC AGTCAGTGCA AAAGACTTCC ATTAGTTTAC CAATATCGCT TCAGGATTCT 
GCTCAGTGTC AAACAGAGCC ACAAAAAGCT GTCATGAGCC CTCCGGGCTC AGCTGTTACG 
TCACCCAATT TGTCAGAAAA AGCCTCAAAG ACTCGAATCC CAATGGGATT TAAAGCCTTT 
CTAAAATCCC CTCCCAGCCA TAAAAGTAGT CCTACAATAG CAGGCAAGCA AGAGAAAGAT 
CATATCAACT CTGTCTCCAA GGAAACTGTG ACTTCAAATG CTTCTACTCA GTGTGACACC 
TCGCAGGCAG CGTACAGTAC GGATTCAACA CCTACCGTGT CTGTTCCCGA AGGGAAAGGT 
GAATTTCTGT GTCAGCCACA GAAAGAGGAC TTACAACCCC CCTTGTCGTC AGCAGAAGGA 
AATGTGAGTG ACAAGGCAAA AAGGAGTAGT CAACTCTTTG GCAGATCCAT ATCTATTAAT 
ACCAAACCAC ACCTAAAGCC AGCTTTAGGG ATGAACGGGG CCAAAGCTCG AAGCCAGAGC 
TTCAGCACCA ACTACATAGA GAAACCCAAC ATCAATGCCA TGGAGGGGCC GGGAAGGATC 
AGGACTCAAA TCATCACTAA CTCAGGTGAA AGGGGGAATT CGCTGTCAAG GCAGAGTTCT 
TCAGAAACAA CTGGGGTTGG ATTGGCTGAA AGTCCAATCC ATGCCCCTAG AACCCGGCTA 
AGCCATCATG GTGGCATGAT GGGACCAAAT AGTCAGAGCG GCCTCCCTGA GAAGTCCATT 
CAGTCGTGTG TTATAGGTGA AGGAAAAGCT TGTGGAGTGA AAGGAGATGT GGTCACCTCT 
CCTTCTCAAA GAGAAGCTCG CAGCTTGCCC AAAAGTGACA GAACTGCCTT TATTAATGTC 
CAAAAGCCGG CAAAAGTGGA CTCCCATCCT CAAATCCAGT CTTCCTCAGC TTTTGTCTTT 
AGTCCTGAAA ATCCTACCAG AGAAGCAGAA ACCATTACCA CCACTCCTGC TGAGCCAGAC 
AAAACCCAGT TGGGTTCCCC AGGCAATTTC CCAGACACAG AGGAGAAAAA AATCAGCCCA 
ACAACGTGCA CTATTGAAGA GAAAGTGATG ATGGGGATAG AAGAAAACCT GCAGAAAAGT 
CAAGAGCAGG AGAAGGCTGC AGCTAATGAG GCCAAACAGA AGACGGGCCC GTCTTTGGCC 
AACTGGTTTG GCCTCCGCAA GAGCAAACTA CCAGCTTTTA GCGGCAAAAA AGCTGATTCC 
CCGAAGGCAA GAGACGAGAA GAAAGAGCTG AAGATTGGGT CTGTGATCAG CGGCAAGCAG 
ATGAAGTCTG ATAAGAAGAA GGAAAGGAAG AAGGATGGTC AGGAGGTCCA GAATTTGTCA 
GAGATGAACA ACAAGCTGAG CTCCATCATG GACCACTGTA ACAATCAGAT GGGTCAGATC 
GCCAGTCAGA TCCAGAGCAC CACTGTGTTA ATCGGCAAGG ACCAGTTTGT AAAAGAGCTT 
CTTGGGAGAG CTACTGTGAA GGGCAACGCT TCGCCACCTG GCATCTCTAC ACCGAAGAAA 
CACTGTGAAA CAAAGGGCGA CATGCAGATC TGTCCAGATA ATACCCTCAT ACTGACGCAA 
AAGATCAGCC TCAAGGCCAA AAACGACGAG GGGAACATTC CGGACACGTC TTGTCAAGAC 
CACATGATAG GCTCCAGCTG TCAGATGAGA ACCTTGGACA GCGGCATTGG AACCTTCCCC 
CTGCCCGATT CGGTCACGCG GGCCAGCAGT CGCCACATCC CCAAATCTGA GTCCAGTCCA 
GATGGGGTGA TGGCCGGCTC AACTGAGCGC AGTCGGGACA CTCCCTCCTC CCACCCTGAC 
CCCTCACAGT CAGATTTCAA AGTGCCTTCC CCTTCAAAAG CCCATGTGCA CACCCCCACT 
ACCAGCATGG GTCACTCCCT GTCTGATCCC ACTGTGACAT GCAGCAGGAA TGCCCTGGAG 
ACCCAGAGTC GCCTGCCCAA GCTGGCATCC ACAGACGCAA TCAAGACCAA AAGGTTGAGT 
CTTTGTGCCC AGCGCAGCGC CGTCTCCGCT TCTGCCGAGG GAGACGGGAA AGAAATGGAG 
AGGAAGATGA AAACAAGCGA GCATGAATCA CCTGGTGAGA CAACATTGCG GGTTTGCGTG 
TATCCAGGCA GCGACAGTGA CACGGAGACT GAATCCGAGG GAACTACAAG CACTTTGGCG 
TCGCCGCTAA GAACCCCCAT GGACAGACCT CAGAAGAACG ACTCTGTCGA GCAGAATGAG 
GAGACCTTGA AAAAACAGAA TGCAGAGAAA TCCCTGTCCA TTATGGACTA CTATCAGCAC 
GATGTGTTCT TGCACCTGGA GACAGACGCC AGGAGGATTT CCCAGTACAC CTTGCTGCAC 
AAAGAGTCGT CCCTCGACGG AGAAGCAGGA GACAGGCTCA GCAAAGAGAT TCCTGTCAGT 
CCCAACCAGC CGGCCTCTTT GGACGTCTCC TTGGAATCGT TGAACAAAAT GAGCCACAGC 
AGCTCCAGCT GCCTGTGCGC GGACGCCAGC CACCGAGCCG GCTGCCCCGT AGAGACGGGG 
AGGACCGCAG AGAGCTGCTG CAAGGTCAGC CAGTCCTCCT GCTCCTCCAG CTTCATCAGC 
AGGCCCGGGG TGGACCCCGC GGGCTCCCTT AGCGACTCCC TCTACGACAG TTTCTCCTCC 
TGCACCAGTC AGGGCTCCAA CGATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020563986.1
CDS	1725..7454
Translation	MNQDAASPSGEKLAVARLQREVARSKSEGTMREKLIHELEEERRLRLESEKRLREVTEESELGRAQMISLQQQFSRMEETVRSLLQNQGVLEQTAVDTVDIMKAYKDKLSEEVQKLQDGPEAKQAEPASTELDGSQADEDRDKTKLLLERLKTLEEENSALASENESQREQYERCLDEVANQVVQALLTQKDLREECLKLRTRVFDLEQQNRALSVLFQQKIKPASDLLLQKLHSRIMDLSAADLLLEPERSKAFLLSRNTDSPSNEVHSNGKPGVPVAKCLSQMSLMAPAPIYSRSSSELSLSSACSEFSSGSYTWNDGRSCGKMSSLTWEKRLSLGSSAPSNICAPLEEQPPTRRKESHILEGLRKLQRRKHRSSSCSSRVSKSGYKDCMNSNEGIYSLGIKSSSKGVSKPVHVSRASAALGRRFSYDSDDADDELARSSCRDNIPTKESWIHCKKLSHSISDSLCSLEGTQEAVGDGTSCPTASKPYDSRERPEKLMSFINSFLPLEGRKSAFSRPTVHHRNPTQVEDPSCRSNADDPEETIPDSVGVKTPCSQPQELAERDIKRLSRDTIKIFKQQFMQKDQGRTQSADGRPKPFSLLKELKATKCTQSEESILAIFDAEGEPIDLCAQRLVSGAEGRKAASCCSEFQKRTTRQNSANTTNYSVLESPEKPSEHQTRSGKGSDSMEGSAERLQLAPQRKLIKPPSSRTNKGFSIPPMSDVNSPKSGGSKIPGVNKPLASPLRVSKGTTTEPNNSGNSGPSAQEKSSSSLTVKMSRFIKTQGTSTQSPKAVNCKVSGQADLSKTSSSTSPHLPKRRLDFSDNSEQPTRDKHCETSKNKLRSPSPPPPPGRTTSLLVRPNYEASPQAHKSGAQPSTPTTVRGPPPSYHTSVAPNMQGTLPIKDKDCLDVDAGYGTALAPQKLVEKTTQHFQKTTAVTQTPTKGTSKRIKDYLPPASSGCARVPEIAPKNSKNVPPPYSALRVPSLQNSFANKKVPAQENTHQSVQKTSISLPISLQDSAQCQTEPQKAVMSPPGSAVTSPNLSEKASKTRIPMGFKAFLKSPPSHKSSPTIAGKQEKDHINSVSKETVTSNASTQCDTSQAAYSTDSTPTVSVPEGKGEFLCQPQKEDLQPPLSSAEGNVSDKAKRSSQLFGRSISINTKPHLKPALGMNGAKARSQSFSTNYIEKPNINAMEGPGRIRTQIITNSGERGNSLSRQSSSETTGVGLAESPIHAPRTRLSHHGGMMGPNSQSGLPEKSIQSCVIGEGKACGVKGDVVTSPSQREARSLPKSDRTAFINVQKPAKVDSHPQIQSSSAFVFSPENPTREAETITTTPAEPDKTQLGSPGNFPDTEEKKISPTTCTIEEKVMMGIEENLQKSQEQEKAAANEAKQKTGPSLANWFGLRKSKLPAFSGKKADSPKARDEKKELKIGSVISGKQMKSDKKKERKKDGQEVQNLSEMNNKLSSIMDHCNNQMGQIASQIQSTTVLIGKDQFVKELLGRATVKGNASPPGISTPKKHCETKGDMQICPDNTLILTQKISLKAKNDEGNIPDTSCQDHMIGSSCQMRTLDSGIGTFPLPDSVTRASSRHIPKSESSPDGVMAGSTERSRDTPSSHPDPSQSDFKVPSPSKAHVHTPTTSMGHSLSDPTVTCSRNALETQSRLPKLASTDAIKTKRLSLCAQRSAVSASAEGDGKEMERKMKTSEHESPGETTLRVCVYPGSDSDTETESEGTTSTLASPLRTPMDRPQKNDSVEQNEETLKKQNAEKSLSIMDYYQHDVFLHLETDARRISQYTLLHKESSLDGEAGDRLSKEIPVSPNQPASLDVSLESLNKMSHSSSSCLCADASHRAGCPVETGRTAESCCKVSQSSCSSSFISRPGVDPAGSLSDSLYDSFSSCTSQGSNDV

Target ORF information:

RefSeq Version	XM_020708327.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020708327.1

ORF Insert Sequence:

ATGAACCAGG ATGCTGCATC TCCATCAGGG GAAAAGCTGG CTGTGGCTCG CCTGCAGAGG 
GAAGTCGCAC GAAGCAAGAG TGAAGGAACA ATGCGAGAAA AGTTGATCCA CGAGCTAGAG 
GAAGAACGAC GCCTGCGACT GGAGAGTGAG AAGCGTCTCC GGGAAGTGAC GGAGGAGTCA 
GAGCTGGGAA GGGCGCAGAT GATTTCACTG CAGCAGCAGT TCTCCAGGAT GGAAGAGACG 
GTGCGGTCGC TGCTGCAGAA CCAGGGCGTC CTGGAGCAGA CTGCTGTGGA CACAGTGGAC 
ATCATGAAGG CCTACAAGGA TAAACTCTCC GAGGAAGTGC AGAAACTGCA GGATGGCCCA 
GAGGCGAAGC AGGCCGAGCC AGCGAGTACC GAGCTCGACG GCAGCCAAGC GGACGAAGAC 
AGAGACAAAA CCAAGCTCCT TCTGGAGCGC CTGAAGACCC TCGAGGAGGA GAACTCGGCG 
TTGGCCTCGG AGAACGAGAG CCAGAGGGAA CAGTACGAGC GTTGCCTGGA CGAGGTCGCT 
AACCAAGTTG TACAGGCACT CCTCACACAG AAGGATCTGA GAGAGGAATG TCTTAAGCTG 
CGGACAAGAG TTTTTGATCT GGAGCAGCAG AATCGAGCAC TGAGTGTTTT GTTCCAGCAG 
AAGATAAAGC CGGCATCAGA CCTCCTCCTT CAGAAACTCC ACTCGCGGAT CATGGATCTG 
TCTGCGGCAG ATTTGCTTCT GGAGCCGGAG AGGAGCAAGG CCTTCCTCCT TTCCAGGAAT 
ACAGACTCCC CTTCCAACGA GGTTCATTCG AATGGAAAGC CAGGTGTCCC TGTGGCCAAA 
TGTCTGAGCC AGATGAGTCT GATGGCGCCC GCTCCCATCT ATTCTCGCAG CAGCAGCGAG 
CTCTCACTGT CGAGCGCGTG CAGCGAGTTC TCCAGCGGCT CCTACACCTG GAATGATGGG 
CGATCCTGCG GGAAAATGTC ATCCCTGACG TGGGAGAAGA GACTCAGTTT GGGCTCGTCA 
GCTCCAAGTA ACATCTGCGC GCCGCTGGAG GAACAGCCCC CCACACGGCG TAAGGAGAGC 
CACATACTGG AGGGCCTGAG AAAACTCCAG CGGAGGAAAC ACAGGAGCTC CTCCTGCTCC 
TCTAGAGTGT CCAAGTCAGG CTACAAAGAT TGCATGAACT CCAACGAGGG CATCTACTCT 
TTGGGTATCA AGAGCAGCAG CAAAGGGGTG TCTAAACCTG TACATGTCAG CAGAGCTTCT 
GCTGCTCTTG GAAGGCGGTT TTCCTACGAT TCCGATGATG CTGACGATGA ACTGGCACGC 
TCTAGTTGCA GAGATAATAT TCCCACTAAG GAGAGCTGGA TTCACTGCAA GAAGCTCTCC 
CACAGCATCT CTGATAGTTT GTGCAGCTTG GAGGGGACAC AGGAGGCTGT GGGTGATGGT 
ACTTCTTGTC CCACAGCTTC AAAACCCTAT GACTCAAGGG AGCGTCCGGA GAAGCTCATG 
AGTTTCATCA ACAGTTTTCT TCCTTTAGAA GGGCGTAAAT CAGCCTTTAG CAGGCCAACC 
GTGCACCACA GAAATCCTAC TCAAGTAGAA GATCCGAGTT GCAGATCCAA CGCAGATGAT 
CCAGAGGAAA CCATTCCTGA TTCTGTGGGG GTCAAGACGC CATGCAGTCA GCCGCAAGAA 
CTAGCAGAGA GAGATATCAA GAGGCTCTCA AGGGATACCA TCAAGATTTT CAAGCAACAG 
TTCATGCAAA AAGATCAGGG GCGCACTCAG TCTGCGGATG GGAGACCGAA GCCTTTCAGC 
CTATTGAAGG AACTTAAAGC AACCAAGTGC ACCCAGTCTG AGGAGAGCAT ACTGGCGATA 
TTTGATGCTG AAGGAGAACC TATTGATCTG TGCGCTCAGA GACTAGTATC AGGAGCAGAA 
GGTCGCAAAG CCGCTAGCTG TTGCTCAGAG TTCCAAAAGA GGACAACAAG GCAAAATTCA 
GCAAACACGA CAAACTATAG CGTTCTTGAA TCTCCAGAAA AACCTTCAGA ACATCAGACT 
AGATCAGGCA AAGGCAGTGA TAGCATGGAA GGAAGTGCAG AAAGGCTACA GTTAGCTCCT 
CAGAGGAAGT TGATAAAGCC ACCAAGCAGT CGAACTAATA AAGGCTTTTC CATTCCTCCC 
ATGAGTGACG TCAACAGCCC CAAATCTGGT GGCTCTAAGA TCCCCGGTGT CAATAAGCCC 
TTAGCTTCTC CGTTAAGAGT ATCCAAGGGC ACAACCACTG AGCCTAATAA CAGTGGAAAC 
TCAGGACCCT CTGCTCAGGA GAAATCTTCG TCTTCTCTTA CGGTTAAGAT GTCTAGATTC 
ATCAAGACGC AGGGAACTTC CACTCAGAGT CCGAAAGCTG TGAACTGCAA GGTGTCTGGT 
CAGGCCGACT TAAGCAAGAC CTCTTCTTCC ACTTCCCCCC ACCTTCCAAA AAGACGCCTC 
GACTTCTCTG ACAACAGCGA ACAGCCGACC AGAGACAAAC ACTGTGAAAC CAGCAAGAAC 
AAACTCAGGT CTCCATCTCC TCCCCCTCCC CCAGGACGCA CCACCTCTTT ACTGGTCAGA 
CCAAATTACG AAGCGTCGCC TCAAGCACAC AAATCAGGGG CTCAACCATC CACGCCAACC 
ACTGTGAGGG GCCCTCCCCC AAGTTACCAC ACCTCAGTTG CACCAAATAT GCAAGGCACG 
CTCCCCATCA AGGATAAAGA CTGTTTAGAC GTAGATGCAG GCTATGGGAC TGCACTTGCA 
CCTCAGAAGC TGGTGGAGAA AACCACCCAG CACTTTCAGA AGACCACTGC CGTAACTCAG 
ACTCCAACTA AAGGCACTTC CAAGCGAATA AAAGACTACC TCCCACCTGC AAGCTCAGGA 
TGTGCCAGAG TACCTGAAAT TGCACCTAAA AACTCGAAGA ATGTCCCTCC TCCCTACAGT 
GCTCTAAGAG TGCCCTCCCT TCAAAACTCC TTTGCAAACA AAAAGGTACC TGCTCAAGAA 
AACACCCATC AGTCAGTGCA AAAGACTTCC ATTAGTTTAC CAATATCGCT TCAGGATTCT 
GCTCAGTGTC AAACAGAGCC ACAAAAAGCT GTCATGAGCC CTCCGGGCTC AGCTGTTACG 
TCACCCAATT TGTCAGAAAA AGCCTCAAAG ACTCGAATCC CAATGGGATT TAAAGCCTTT 
CTAAAATCCC CTCCCAGCCA TAAAAGTAGT CCTACAATAG CAGGCAAGCA AGAGAAAGAT 
CATATCAACT CTGTCTCCAA GGAAACTGTG ACTTCAAATG CTTCTACTCA GTGTGACACC 
TCGCAGGCAG CGTACAGTAC GGATTCAACA CCTACCGTGT CTGTTCCCGA AGGGAAAGGT 
GAATTTCTGT GTCAGCCACA GAAAGAGGAC TTACAACCCC CCTTGTCGTC AGCAGAAGGA 
AATGTGAGTG ACAAGGCAAA AAGGAGTAGT CAACTCTTTG GCAGATCCAT ATCTATTAAT 
ACCAAACCAC ACCTAAAGCC AGCTTTAGGG ATGAACGGGG CCAAAGCTCG AAGCCAGAGC 
TTCAGCACCA ACTACATAGA GAAACCCAAC ATCAATGCCA TGGAGGGGCC GGGAAGGATC 
AGGACTCAAA TCATCACTAA CTCAGGTGAA AGGGGGAATT CGCTGTCAAG GCAGAGTTCT 
TCAGAAACAA CTGGGGTTGG ATTGGCTGAA AGTCCAATCC ATGCCCCTAG AACCCGGCTA 
AGCCATCATG GTGGCATGAT GGGACCAAAT AGTCAGAGCG GCCTCCCTGA GAAGTCCATT 
CAGTCGTGTG TTATAGGTGA AGGAAAAGCT TGTGGAGTGA AAGGAGATGT GGTCACCTCT 
CCTTCTCAAA GAGAAGCTCG CAGCTTGCCC AAAAGTGACA GAACTGCCTT TATTAATGTC 
CAAAAGCCGG CAAAAGTGGA CTCCCATCCT CAAATCCAGT CTTCCTCAGC TTTTGTCTTT 
AGTCCTGAAA ATCCTACCAG AGAAGCAGAA ACCATTACCA CCACTCCTGC TGAGCCAGAC 
AAAACCCAGT TGGGTTCCCC AGGCAATTTC CCAGACACAG AGGAGAAAAA AATCAGCCCA 
ACAACGTGCA CTATTGAAGA GAAAGTGATG ATGGGGATAG AAGAAAACCT GCAGAAAAGT 
CAAGAGCAGG AGAAGGCTGC AGCTAATGAG GCCAAACAGA AGACGGGCCC GTCTTTGGCC 
AACTGGTTTG GCCTCCGCAA GAGCAAACTA CCAGCTTTTA GCGGCAAAAA AGCTGATTCC 
CCGAAGGCAA GAGACGAGAA GAAAGAGCTG AAGATTGGGT CTGTGATCAG CGGCAAGCAG 
ATGAAGTCTG ATAAGAAGAA GGAAAGGAAG AAGGATGGTC AGGAGGTCCA GAATTTGTCA 
GAGATGAACA ACAAGCTGAG CTCCATCATG GACCACTGTA ACAATCAGAT GGGTCAGATC 
GCCAGTCAGA TCCAGAGCAC CACTGTGTTA ATCGGCAAGG ACCAGTTTGT AAAAGAGCTT 
CTTGGGAGAG CTACTGTGAA GGGCAACGCT TCGCCACCTG GCATCTCTAC ACCGAAGAAA 
CACTGTGAAA CAAAGGGCGA CATGCAGATC TGTCCAGATA ATACCCTCAT ACTGACGCAA 
AAGATCAGCC TCAAGGCCAA AAACGACGAG GGGAACATTC CGGACACGTC TTGTCAAGAC 
CACATGATAG GCTCCAGCTG TCAGATGAGA ACCTTGGACA GCGGCATTGG AACCTTCCCC 
CTGCCCGATT CGGTCACGCG GGCCAGCAGT CGCCACATCC CCAAATCTGA GTCCAGTCCA 
GATGGGGTGA TGGCCGGCTC AACTGAGCGC AGTCGGGACA CTCCCTCCTC CCACCCTGAC 
CCCTCACAGT CAGATTTCAA AGTGCCTTCC CCTTCAAAAG CCCATGTGCA CACCCCCACT 
ACCAGCATGG GTCACTCCCT GTCTGATCCC ACTGTGACAT GCAGCAGGAA TGCCCTGGAG 
ACCCAGAGTC GCCTGCCCAA GCTGGCATCC ACAGACGCAA TCAAGACCAA AAGGTTGAGT 
CTTTGTGCCC AGCGCAGCGC CGTCTCCGCT TCTGCCGAGG GAGACGGGAA AGAAATGGAG 
AGGAAGATGA AAACAAGCGA GCATGAATCA CCTGGTGAGA CAACATTGCG GGTTTGCGTG 
TATCCAGGCA GCGACAGTGA CACGGAGACT GAATCCGAGG GAACTACAAG CACTTTGGCG 
TCGCCGCTAA GAACCCCCAT GGACAGACCT CAGAAGAACG ACTCTGTCGA GCAGAATGAG 
GAGACCTTGA AAAAACAGAA TGCAGAGAAA TCCCTGTCCA TTATGGACTA CTATCAGCAC 
GATGTGTTCT TGCACCTGGA GACAGACGCC AGGAGGATTT CCCAGTACAC CTTGCTGCAC 
AAAGAGTCGT CCCTCGACGG AGAAGCAGGA GACAGGCTCA GCAAAGAGAT TCCTGTCAGT 
CCCAACCAGC CGGCCTCTTT GGACGTCTCC TTGGAATCGT TGAACAAAAT GAGCCACAGC 
AGCTCCAGCT GCCTGTGCGC GGACGCCAGC CACCGAGCCG GCTGCCCCGT AGAGACGGGG 
AGGACCGCAG AGAGCTGCTG CAAGGTCAGC CAGTCCTCCT GCTCCTCCAG CTTCATCAGC 
AGGCCCGGGG TGGACCCCGC GGGCTCCCTT AGCGACTCCC TCTACGACAG TTTCTCCTCC 
TGCACCAGTC AGGGCTCCAA CGATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101162908 ( XM_020708327.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020563986.1 Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OJa74624
Clone ID Related Accession (Same CDS sequence)	XM_020708327.1 , XM_020708327.2
Accession Version	XM_020708327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	nck-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020708327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG ATGCTGCATC TCCATCAGGG GAAAAGCTGG CTGTGGCTCG CCTGCAGAGG 
GAAGTCGCAC GAAGCAAGAG TGAAGGAACA ATGCGAGAAA AGTTGATCCA CGAGCTAGAG 
GAAGAACGAC GCCTGCGACT GGAGAGTGAG AAGCGTCTCC GGGAAGTGAC GGAGGAGTCA 
GAGCTGGGAA GGGCGCAGAT GATTTCACTG CAGCAGCAGT TCTCCAGGAT GGAAGAGACG 
GTGCGGTCGC TGCTGCAGAA CCAGGGCGTC CTGGAGCAGA CTGCTGTGGA CACAGTGGAC 
ATCATGAAGG CCTACAAGGA TAAACTCTCC GAGGAAGTGC AGAAACTGCA GGATGGCCCA 
GAGGCGAAGC AGGCCGAGCC AGCGAGTACC GAGCTCGACG GCAGCCAAGC GGACGAAGAC 
AGAGACAAAA CCAAGCTCCT TCTGGAGCGC CTGAAGACCC TCGAGGAGGA GAACTCGGCG 
TTGGCCTCGG AGAACGAGAG CCAGAGGGAA CAGTACGAGC GTTGCCTGGA CGAGGTCGCT 
AACCAAGTTG TACAGGCACT CCTCACACAG AAGGATCTGA GAGAGGAATG TCTTAAGCTG 
CGGACAAGAG TTTTTGATCT GGAGCAGCAG AATCGAGCAC TGAGTGTTTT GTTCCAGCAG 
AAGATAAAGC CGGCATCAGA CCTCCTCCTT CAGAAACTCC ACTCGCGGAT CATGGATCTG 
TCTGCGGCAG ATTTGCTTCT GGAGCCGGAG AGGAGCAAGG CCTTCCTCCT TTCCAGGAAT 
ACAGACTCCC CTTCCAACGA GGTTCATTCG AATGGAAAGC CAGGTGTCCC TGTGGCCAAA 
TGTCTGAGCC AGATGAGTCT GATGGCGCCC GCTCCCATCT ATTCTCGCAG CAGCAGCGAG 
CTCTCACTGT CGAGCGCGTG CAGCGAGTTC TCCAGCGGCT CCTACACCTG GAATGATGGG 
CGATCCTGCG GGAAAATGTC ATCCCTGACG TGGGAGAAGA GACTCAGTTT GGGCTCGTCA 
GCTCCAAGTA ACATCTGCGC GCCGCTGGAG GAACAGCCCC CCACACGGCG TAAGGAGAGC 
CACATACTGG AGGGCCTGAG AAAACTCCAG CGGAGGAAAC ACAGGAGCTC CTCCTGCTCC 
TCTAGAGTGT CCAAGTCAGG CTACAAAGAT TGCATGAACT CCAACGAGGG CATCTACTCT 
TTGGGTATCA AGAGCAGCAG CAAAGGGGTG TCTAAACCTG TACATGTCAG CAGAGCTTCT 
GCTGCTCTTG GAAGGCGGTT TTCCTACGAT TCCGATGATG CTGACGATGA ACTGGCACGC 
TCTAGTTGCA GAGATAATAT TCCCACTAAG GAGAGCTGGA TTCACTGCAA GAAGCTCTCC 
CACAGCATCT CTGATAGTTT GTGCAGCTTG GAGGGGACAC AGGAGGCTGT GGGTGATGGT 
ACTTCTTGTC CCACAGCTTC AAAACCCTAT GACTCAAGGG AGCGTCCGGA GAAGCTCATG 
AGTTTCATCA ACAGTTTTCT TCCTTTAGAA GGGCGTAAAT CAGCCTTTAG CAGGCCAACC 
GTGCACCACA GAAATCCTAC TCAAGTAGAA GATCCGAGTT GCAGATCCAA CGCAGATGAT 
CCAGAGGAAA CCATTCCTGA TTCTGTGGGG GTCAAGACGC CATGCAGTCA GCCGCAAGAA 
CTAGCAGAGA GAGATATCAA GAGGCTCTCA AGGGATACCA TCAAGATTTT CAAGCAACAG 
TTCATGCAAA AAGATCAGGG GCGCACTCAG TCTGCGGATG GGAGACCGAA GCCTTTCAGC 
CTATTGAAGG AACTTAAAGC AACCAAGTGC ACCCAGTCTG AGGAGAGCAT ACTGGCGATA 
TTTGATGCTG AAGGAGAACC TATTGATCTG TGCGCTCAGA GACTAGTATC AGGAGCAGAA 
GGTCGCAAAG CCGCTAGCTG TTGCTCAGAG TTCCAAAAGA GGACAACAAG GCAAAATTCA 
GCAAACACGA CAAACTATAG CGTTCTTGAA TCTCCAGAAA AACCTTCAGA ACATCAGACT 
AGATCAGGCA AAGGCAGTGA TAGCATGGAA GGAAGTGCAG AAAGGCTACA GTTAGCTCCT 
CAGAGGAAGT TGATAAAGCC ACCAAGCAGT CGAACTAATA AAGGCTTTTC CATTCCTCCC 
ATGAGTGACG TCAACAGCCC CAAATCTGGT GGCTCTAAGA TCCCCGGTGT CAATAAGCCC 
TTAGCTTCTC CGTTAAGAGT ATCCAAGGGC ACAACCACTG AGCCTAATAA CAGTGGAAAC 
TCAGGACCCT CTGCTCAGGA GAAATCTTCG TCTTCTCTTA CGGTTAAGAT GTCTAGATTC 
ATCAAGACGC AGGGAACTTC CACTCAGAGT CCGAAAGCTG TGAACTGCAA GGTGTCTGGT 
CAGGCCGACT TAAGCAAGAC CTCTTCTTCC ACTTCCCCCC ACCTTCCAAA AAGACGCCTC 
GACTTCTCTG ACAACAGCGA ACAGCCGACC AGAGACAAAC ACTGTGAAAC CAGCAAGAAC 
AAACTCAGGT CTCCATCTCC TCCCCCTCCC CCAGGACGCA CCACCTCTTT ACTGGTCAGA 
CCAAATTACG AAGCGTCGCC TCAAGCACAC AAATCAGGGG CTCAACCATC CACGCCAACC 
ACTGTGAGGG GCCCTCCCCC AAGTTACCAC ACCTCAGTTG CACCAAATAT GCAAGGCACG 
CTCCCCATCA AGGATAAAGA CTGTTTAGAC GTAGATGCAG GCTATGGGAC TGCACTTGCA 
CCTCAGAAGC TGGTGGAGAA AACCACCCAG CACTTTCAGA AGACCACTGC CGTAACTCAG 
ACTCCAACTA AAGGCACTTC CAAGCGAATA AAAGACTACC TCCCACCTGC AAGCTCAGGA 
TGTGCCAGAG TACCTGAAAT TGCACCTAAA AACTCGAAGA ATGTCCCTCC TCCCTACAGT 
GCTCTAAGAG TGCCCTCCCT TCAAAACTCC TTTGCAAACA AAAAGGTACC TGCTCAAGAA 
AACACCCATC AGTCAGTGCA AAAGACTTCC ATTAGTTTAC CAATATCGCT TCAGGATTCT 
GCTCAGTGTC AAACAGAGCC ACAAAAAGCT GTCATGAGCC CTCCGGGCTC AGCTGTTACG 
TCACCCAATT TGTCAGAAAA AGCCTCAAAG ACTCGAATCC CAATGGGATT TAAAGCCTTT 
CTAAAATCCC CTCCCAGCCA TAAAAGTAGT CCTACAATAG CAGGCAAGCA AGAGAAAGAT 
CATATCAACT CTGTCTCCAA GGAAACTGTG ACTTCAAATG CTTCTACTCA GTGTGACACC 
TCGCAGGCAG CGTACAGTAC GGATTCAACA CCTACCGTGT CTGTTCCCGA AGGGAAAGGT 
GAATTTCTGT GTCAGCCACA GAAAGAGGAC TTACAACCCC CCTTGTCGTC AGCAGAAGGA 
AATGTGAGTG ACAAGGCAAA AAGGAGTAGT CAACTCTTTG GCAGATCCAT ATCTATTAAT 
ACCAAACCAC ACCTAAAGCC AGCTTTAGGG ATGAACGGGG CCAAAGCTCG AAGCCAGAGC 
TTCAGCACCA ACTACATAGA GAAACCCAAC ATCAATGCCA TGGAGGGGCC GGGAAGGATC 
AGGACTCAAA TCATCACTAA CTCAGGTGAA AGGGGGAATT CGCTGTCAAG GCAGAGTTCT 
TCAGAAACAA CTGGGGTTGG ATTGGCTGAA AGTCCAATCC ATGCCCCTAG AACCCGGCTA 
AGCCATCATG GTGGCATGAT GGGACCAAAT AGTCAGAGCG GCCTCCCTGA GAAGTCCATT 
CAGTCGTGTG TTATAGGTGA AGGAAAAGCT TGTGGAGTGA AAGGAGATGT GGTCACCTCT 
CCTTCTCAAA GAGAAGCTCG CAGCTTGCCC AAAAGTGACA GAACTGCCTT TATTAATGTC 
CAAAAGCCGG CAAAAGTGGA CTCCCATCCT CAAATCCAGT CTTCCTCAGC TTTTGTCTTT 
AGTCCTGAAA ATCCTACCAG AGAAGCAGAA ACCATTACCA CCACTCCTGC TGAGCCAGAC 
AAAACCCAGT TGGGTTCCCC AGGCAATTTC CCAGACACAG AGGAGAAAAA AATCAGCCCA 
ACAACGTGCA CTATTGAAGA GAAAGTGATG ATGGGGATAG AAGAAAACCT GCAGAAAAGT 
CAAGAGCAGG AGAAGGCTGC AGCTAATGAG GCCAAACAGA AGACGGGCCC GTCTTTGGCC 
AACTGGTTTG GCCTCCGCAA GAGCAAACTA CCAGCTTTTA GCGGCAAAAA AGCTGATTCC 
CCGAAGGCAA GAGACGAGAA GAAAGAGCTG AAGATTGGGT CTGTGATCAG CGGCAAGCAG 
ATGAAGTCTG ATAAGAAGAA GGAAAGGAAG AAGGATGGTC AGGAGGTCCA GAATTTGTCA 
GAGATGAACA ACAAGCTGAG CTCCATCATG GACCACTGTA ACAATCAGAT GGGTCAGATC 
GCCAGTCAGA TCCAGAGCAC CACTGTGTTA ATCGGCAAGG ACCAGTTTGT AAAAGAGCTT 
CTTGGGAGAG CTACTGTGAA GGGCAACGCT TCGCCACCTG GCATCTCTAC ACCGAAGAAA 
CACTGTGAAA CAAAGGGCGA CATGCAGATC TGTCCAGATA ATACCCTCAT ACTGACGCAA 
AAGATCAGCC TCAAGGCCAA AAACGACGAG GGGAACATTC CGGACACGTC TTGTCAAGAC 
CACATGATAG GCTCCAGCTG TCAGATGAGA ACCTTGGACA GCGGCATTGG AACCTTCCCC 
CTGCCCGATT CGGTCACGCG GGCCAGCAGT CGCCACATCC CCAAATCTGA GTCCAGTCCA 
GATGGGGTGA TGGCCGGCTC AACTGAGCGC AGTCGGGACA CTCCCTCCTC CCACCCTGAC 
CCCTCACAGT CAGATTTCAA AGTGCCTTCC CCTTCAAAAG CCCATGTGCA CACCCCCACT 
ACCAGCATGG GTCACTCCCT GTCTGATCCC ACTGTGACAT GCAGCAGGAA TGCCCTGGAG 
ACCCAGAGTC GCCTGCCCAA GCTGGCATCC ACAGACGCAA TCAAGACCAA AAGGTTGAGT 
CTTTGTGCCC AGCGCAGCGC CGTCTCCGCT TCTGCCGAGG GAGACGGGAA AGAAATGGAG 
AGGAAGATGA AAACAAGCGA GCATGAATCA CCTGGTGAGA CAACATTGCG GGTTTGCGTG 
TATCCAGGCA GCGACAGTGA CACGGAGACT GAATCCGAGG GAACTACAAG CACTTTGGCG 
TCGCCGCTAA GAACCCCCAT GGACAGACCT CAGAAGAACG ACTCTGTCGA GCAGAATGAG 
GAGACCTTGA AAAAACAGAA TGCAGAGAAA TCCCTGTCCA TTATGGACTA CTATCAGCAC 
GATGTGTTCT TGCACCTGGA GACAGACGCC AGGAGGATTT CCCAGTACAC CTTGCTGCAC 
AAAGAGTCGT CCCTCGACGG AGAAGCAGGA GACAGGCTCA GCAAAGAGAT TCCTGTCAGT 
CCCAACCAGC CGGCCTCTTT GGACGTCTCC TTGGAATCGT TGAACAAAAT GAGCCACAGC 
AGCTCCAGCT GCCTGTGCGC GGACGCCAGC CACCGAGCCG GCTGCCCCGT AGAGACGGGG 
AGGACCGCAG AGAGCTGCTG CAAGGTCAGC CAGTCCTCCT GCTCCTCCAG CTTCATCAGC 
AGGCCCGGGG TGGACCCCGC GGGCTCCCTT AGCGACTCCC TCTACGACAG TTTCTCCTCC 
TGCACCAGTC AGGGCTCCAA CGATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020563986.1
CDS	1733..7462
Translation	MNQDAASPSGEKLAVARLQREVARSKSEGTMREKLIHELEEERRLRLESEKRLREVTEESELGRAQMISLQQQFSRMEETVRSLLQNQGVLEQTAVDTVDIMKAYKDKLSEEVQKLQDGPEAKQAEPASTELDGSQADEDRDKTKLLLERLKTLEEENSALASENESQREQYERCLDEVANQVVQALLTQKDLREECLKLRTRVFDLEQQNRALSVLFQQKIKPASDLLLQKLHSRIMDLSAADLLLEPERSKAFLLSRNTDSPSNEVHSNGKPGVPVAKCLSQMSLMAPAPIYSRSSSELSLSSACSEFSSGSYTWNDGRSCGKMSSLTWEKRLSLGSSAPSNICAPLEEQPPTRRKESHILEGLRKLQRRKHRSSSCSSRVSKSGYKDCMNSNEGIYSLGIKSSSKGVSKPVHVSRASAALGRRFSYDSDDADDELARSSCRDNIPTKESWIHCKKLSHSISDSLCSLEGTQEAVGDGTSCPTASKPYDSRERPEKLMSFINSFLPLEGRKSAFSRPTVHHRNPTQVEDPSCRSNADDPEETIPDSVGVKTPCSQPQELAERDIKRLSRDTIKIFKQQFMQKDQGRTQSADGRPKPFSLLKELKATKCTQSEESILAIFDAEGEPIDLCAQRLVSGAEGRKAASCCSEFQKRTTRQNSANTTNYSVLESPEKPSEHQTRSGKGSDSMEGSAERLQLAPQRKLIKPPSSRTNKGFSIPPMSDVNSPKSGGSKIPGVNKPLASPLRVSKGTTTEPNNSGNSGPSAQEKSSSSLTVKMSRFIKTQGTSTQSPKAVNCKVSGQADLSKTSSSTSPHLPKRRLDFSDNSEQPTRDKHCETSKNKLRSPSPPPPPGRTTSLLVRPNYEASPQAHKSGAQPSTPTTVRGPPPSYHTSVAPNMQGTLPIKDKDCLDVDAGYGTALAPQKLVEKTTQHFQKTTAVTQTPTKGTSKRIKDYLPPASSGCARVPEIAPKNSKNVPPPYSALRVPSLQNSFANKKVPAQENTHQSVQKTSISLPISLQDSAQCQTEPQKAVMSPPGSAVTSPNLSEKASKTRIPMGFKAFLKSPPSHKSSPTIAGKQEKDHINSVSKETVTSNASTQCDTSQAAYSTDSTPTVSVPEGKGEFLCQPQKEDLQPPLSSAEGNVSDKAKRSSQLFGRSISINTKPHLKPALGMNGAKARSQSFSTNYIEKPNINAMEGPGRIRTQIITNSGERGNSLSRQSSSETTGVGLAESPIHAPRTRLSHHGGMMGPNSQSGLPEKSIQSCVIGEGKACGVKGDVVTSPSQREARSLPKSDRTAFINVQKPAKVDSHPQIQSSSAFVFSPENPTREAETITTTPAEPDKTQLGSPGNFPDTEEKKISPTTCTIEEKVMMGIEENLQKSQEQEKAAANEAKQKTGPSLANWFGLRKSKLPAFSGKKADSPKARDEKKELKIGSVISGKQMKSDKKKERKKDGQEVQNLSEMNNKLSSIMDHCNNQMGQIASQIQSTTVLIGKDQFVKELLGRATVKGNASPPGISTPKKHCETKGDMQICPDNTLILTQKISLKAKNDEGNIPDTSCQDHMIGSSCQMRTLDSGIGTFPLPDSVTRASSRHIPKSESSPDGVMAGSTERSRDTPSSHPDPSQSDFKVPSPSKAHVHTPTTSMGHSLSDPTVTCSRNALETQSRLPKLASTDAIKTKRLSLCAQRSAVSASAEGDGKEMERKMKTSEHESPGETTLRVCVYPGSDSDTETESEGTTSTLASPLRTPMDRPQKNDSVEQNEETLKKQNAEKSLSIMDYYQHDVFLHLETDARRISQYTLLHKESSLDGEAGDRLSKEIPVSPNQPASLDVSLESLNKMSHSSSSCLCADASHRAGCPVETGRTAESCCKVSQSSCSSSFISRPGVDPAGSLSDSLYDSFSSCTSQGSNDV

Target ORF information:

RefSeq Version	XM_020708327.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020708327.2

ORF Insert Sequence:

ATGAACCAGG ATGCTGCATC TCCATCAGGG GAAAAGCTGG CTGTGGCTCG CCTGCAGAGG 
GAAGTCGCAC GAAGCAAGAG TGAAGGAACA ATGCGAGAAA AGTTGATCCA CGAGCTAGAG 
GAAGAACGAC GCCTGCGACT GGAGAGTGAG AAGCGTCTCC GGGAAGTGAC GGAGGAGTCA 
GAGCTGGGAA GGGCGCAGAT GATTTCACTG CAGCAGCAGT TCTCCAGGAT GGAAGAGACG 
GTGCGGTCGC TGCTGCAGAA CCAGGGCGTC CTGGAGCAGA CTGCTGTGGA CACAGTGGAC 
ATCATGAAGG CCTACAAGGA TAAACTCTCC GAGGAAGTGC AGAAACTGCA GGATGGCCCA 
GAGGCGAAGC AGGCCGAGCC AGCGAGTACC GAGCTCGACG GCAGCCAAGC GGACGAAGAC 
AGAGACAAAA CCAAGCTCCT TCTGGAGCGC CTGAAGACCC TCGAGGAGGA GAACTCGGCG 
TTGGCCTCGG AGAACGAGAG CCAGAGGGAA CAGTACGAGC GTTGCCTGGA CGAGGTCGCT 
AACCAAGTTG TACAGGCACT CCTCACACAG AAGGATCTGA GAGAGGAATG TCTTAAGCTG 
CGGACAAGAG TTTTTGATCT GGAGCAGCAG AATCGAGCAC TGAGTGTTTT GTTCCAGCAG 
AAGATAAAGC CGGCATCAGA CCTCCTCCTT CAGAAACTCC ACTCGCGGAT CATGGATCTG 
TCTGCGGCAG ATTTGCTTCT GGAGCCGGAG AGGAGCAAGG CCTTCCTCCT TTCCAGGAAT 
ACAGACTCCC CTTCCAACGA GGTTCATTCG AATGGAAAGC CAGGTGTCCC TGTGGCCAAA 
TGTCTGAGCC AGATGAGTCT GATGGCGCCC GCTCCCATCT ATTCTCGCAG CAGCAGCGAG 
CTCTCACTGT CGAGCGCGTG CAGCGAGTTC TCCAGCGGCT CCTACACCTG GAATGATGGG 
CGATCCTGCG GGAAAATGTC ATCCCTGACG TGGGAGAAGA GACTCAGTTT GGGCTCGTCA 
GCTCCAAGTA ACATCTGCGC GCCGCTGGAG GAACAGCCCC CCACACGGCG TAAGGAGAGC 
CACATACTGG AGGGCCTGAG AAAACTCCAG CGGAGGAAAC ACAGGAGCTC CTCCTGCTCC 
TCTAGAGTGT CCAAGTCAGG CTACAAAGAT TGCATGAACT CCAACGAGGG CATCTACTCT 
TTGGGTATCA AGAGCAGCAG CAAAGGGGTG TCTAAACCTG TACATGTCAG CAGAGCTTCT 
GCTGCTCTTG GAAGGCGGTT TTCCTACGAT TCCGATGATG CTGACGATGA ACTGGCACGC 
TCTAGTTGCA GAGATAATAT TCCCACTAAG GAGAGCTGGA TTCACTGCAA GAAGCTCTCC 
CACAGCATCT CTGATAGTTT GTGCAGCTTG GAGGGGACAC AGGAGGCTGT GGGTGATGGT 
ACTTCTTGTC CCACAGCTTC AAAACCCTAT GACTCAAGGG AGCGTCCGGA GAAGCTCATG 
AGTTTCATCA ACAGTTTTCT TCCTTTAGAA GGGCGTAAAT CAGCCTTTAG CAGGCCAACC 
GTGCACCACA GAAATCCTAC TCAAGTAGAA GATCCGAGTT GCAGATCCAA CGCAGATGAT 
CCAGAGGAAA CCATTCCTGA TTCTGTGGGG GTCAAGACGC CATGCAGTCA GCCGCAAGAA 
CTAGCAGAGA GAGATATCAA GAGGCTCTCA AGGGATACCA TCAAGATTTT CAAGCAACAG 
TTCATGCAAA AAGATCAGGG GCGCACTCAG TCTGCGGATG GGAGACCGAA GCCTTTCAGC 
CTATTGAAGG AACTTAAAGC AACCAAGTGC ACCCAGTCTG AGGAGAGCAT ACTGGCGATA 
TTTGATGCTG AAGGAGAACC TATTGATCTG TGCGCTCAGA GACTAGTATC AGGAGCAGAA 
GGTCGCAAAG CCGCTAGCTG TTGCTCAGAG TTCCAAAAGA GGACAACAAG GCAAAATTCA 
GCAAACACGA CAAACTATAG CGTTCTTGAA TCTCCAGAAA AACCTTCAGA ACATCAGACT 
AGATCAGGCA AAGGCAGTGA TAGCATGGAA GGAAGTGCAG AAAGGCTACA GTTAGCTCCT 
CAGAGGAAGT TGATAAAGCC ACCAAGCAGT CGAACTAATA AAGGCTTTTC CATTCCTCCC 
ATGAGTGACG TCAACAGCCC CAAATCTGGT GGCTCTAAGA TCCCCGGTGT CAATAAGCCC 
TTAGCTTCTC CGTTAAGAGT ATCCAAGGGC ACAACCACTG AGCCTAATAA CAGTGGAAAC 
TCAGGACCCT CTGCTCAGGA GAAATCTTCG TCTTCTCTTA CGGTTAAGAT GTCTAGATTC 
ATCAAGACGC AGGGAACTTC CACTCAGAGT CCGAAAGCTG TGAACTGCAA GGTGTCTGGT 
CAGGCCGACT TAAGCAAGAC CTCTTCTTCC ACTTCCCCCC ACCTTCCAAA AAGACGCCTC 
GACTTCTCTG ACAACAGCGA ACAGCCGACC AGAGACAAAC ACTGTGAAAC CAGCAAGAAC 
AAACTCAGGT CTCCATCTCC TCCCCCTCCC CCAGGACGCA CCACCTCTTT ACTGGTCAGA 
CCAAATTACG AAGCGTCGCC TCAAGCACAC AAATCAGGGG CTCAACCATC CACGCCAACC 
ACTGTGAGGG GCCCTCCCCC AAGTTACCAC ACCTCAGTTG CACCAAATAT GCAAGGCACG 
CTCCCCATCA AGGATAAAGA CTGTTTAGAC GTAGATGCAG GCTATGGGAC TGCACTTGCA 
CCTCAGAAGC TGGTGGAGAA AACCACCCAG CACTTTCAGA AGACCACTGC CGTAACTCAG 
ACTCCAACTA AAGGCACTTC CAAGCGAATA AAAGACTACC TCCCACCTGC AAGCTCAGGA 
TGTGCCAGAG TACCTGAAAT TGCACCTAAA AACTCGAAGA ATGTCCCTCC TCCCTACAGT 
GCTCTAAGAG TGCCCTCCCT TCAAAACTCC TTTGCAAACA AAAAGGTACC TGCTCAAGAA 
AACACCCATC AGTCAGTGCA AAAGACTTCC ATTAGTTTAC CAATATCGCT TCAGGATTCT 
GCTCAGTGTC AAACAGAGCC ACAAAAAGCT GTCATGAGCC CTCCGGGCTC AGCTGTTACG 
TCACCCAATT TGTCAGAAAA AGCCTCAAAG ACTCGAATCC CAATGGGATT TAAAGCCTTT 
CTAAAATCCC CTCCCAGCCA TAAAAGTAGT CCTACAATAG CAGGCAAGCA AGAGAAAGAT 
CATATCAACT CTGTCTCCAA GGAAACTGTG ACTTCAAATG CTTCTACTCA GTGTGACACC 
TCGCAGGCAG CGTACAGTAC GGATTCAACA CCTACCGTGT CTGTTCCCGA AGGGAAAGGT 
GAATTTCTGT GTCAGCCACA GAAAGAGGAC TTACAACCCC CCTTGTCGTC AGCAGAAGGA 
AATGTGAGTG ACAAGGCAAA AAGGAGTAGT CAACTCTTTG GCAGATCCAT ATCTATTAAT 
ACCAAACCAC ACCTAAAGCC AGCTTTAGGG ATGAACGGGG CCAAAGCTCG AAGCCAGAGC 
TTCAGCACCA ACTACATAGA GAAACCCAAC ATCAATGCCA TGGAGGGGCC GGGAAGGATC 
AGGACTCAAA TCATCACTAA CTCAGGTGAA AGGGGGAATT CGCTGTCAAG GCAGAGTTCT 
TCAGAAACAA CTGGGGTTGG ATTGGCTGAA AGTCCAATCC ATGCCCCTAG AACCCGGCTA 
AGCCATCATG GTGGCATGAT GGGACCAAAT AGTCAGAGCG GCCTCCCTGA GAAGTCCATT 
CAGTCGTGTG TTATAGGTGA AGGAAAAGCT TGTGGAGTGA AAGGAGATGT GGTCACCTCT 
CCTTCTCAAA GAGAAGCTCG CAGCTTGCCC AAAAGTGACA GAACTGCCTT TATTAATGTC 
CAAAAGCCGG CAAAAGTGGA CTCCCATCCT CAAATCCAGT CTTCCTCAGC TTTTGTCTTT 
AGTCCTGAAA ATCCTACCAG AGAAGCAGAA ACCATTACCA CCACTCCTGC TGAGCCAGAC 
AAAACCCAGT TGGGTTCCCC AGGCAATTTC CCAGACACAG AGGAGAAAAA AATCAGCCCA 
ACAACGTGCA CTATTGAAGA GAAAGTGATG ATGGGGATAG AAGAAAACCT GCAGAAAAGT 
CAAGAGCAGG AGAAGGCTGC AGCTAATGAG GCCAAACAGA AGACGGGCCC GTCTTTGGCC 
AACTGGTTTG GCCTCCGCAA GAGCAAACTA CCAGCTTTTA GCGGCAAAAA AGCTGATTCC 
CCGAAGGCAA GAGACGAGAA GAAAGAGCTG AAGATTGGGT CTGTGATCAG CGGCAAGCAG 
ATGAAGTCTG ATAAGAAGAA GGAAAGGAAG AAGGATGGTC AGGAGGTCCA GAATTTGTCA 
GAGATGAACA ACAAGCTGAG CTCCATCATG GACCACTGTA ACAATCAGAT GGGTCAGATC 
GCCAGTCAGA TCCAGAGCAC CACTGTGTTA ATCGGCAAGG ACCAGTTTGT AAAAGAGCTT 
CTTGGGAGAG CTACTGTGAA GGGCAACGCT TCGCCACCTG GCATCTCTAC ACCGAAGAAA 
CACTGTGAAA CAAAGGGCGA CATGCAGATC TGTCCAGATA ATACCCTCAT ACTGACGCAA 
AAGATCAGCC TCAAGGCCAA AAACGACGAG GGGAACATTC CGGACACGTC TTGTCAAGAC 
CACATGATAG GCTCCAGCTG TCAGATGAGA ACCTTGGACA GCGGCATTGG AACCTTCCCC 
CTGCCCGATT CGGTCACGCG GGCCAGCAGT CGCCACATCC CCAAATCTGA GTCCAGTCCA 
GATGGGGTGA TGGCCGGCTC AACTGAGCGC AGTCGGGACA CTCCCTCCTC CCACCCTGAC 
CCCTCACAGT CAGATTTCAA AGTGCCTTCC CCTTCAAAAG CCCATGTGCA CACCCCCACT 
ACCAGCATGG GTCACTCCCT GTCTGATCCC ACTGTGACAT GCAGCAGGAA TGCCCTGGAG 
ACCCAGAGTC GCCTGCCCAA GCTGGCATCC ACAGACGCAA TCAAGACCAA AAGGTTGAGT 
CTTTGTGCCC AGCGCAGCGC CGTCTCCGCT TCTGCCGAGG GAGACGGGAA AGAAATGGAG 
AGGAAGATGA AAACAAGCGA GCATGAATCA CCTGGTGAGA CAACATTGCG GGTTTGCGTG 
TATCCAGGCA GCGACAGTGA CACGGAGACT GAATCCGAGG GAACTACAAG CACTTTGGCG 
TCGCCGCTAA GAACCCCCAT GGACAGACCT CAGAAGAACG ACTCTGTCGA GCAGAATGAG 
GAGACCTTGA AAAAACAGAA TGCAGAGAAA TCCCTGTCCA TTATGGACTA CTATCAGCAC 
GATGTGTTCT TGCACCTGGA GACAGACGCC AGGAGGATTT CCCAGTACAC CTTGCTGCAC 
AAAGAGTCGT CCCTCGACGG AGAAGCAGGA GACAGGCTCA GCAAAGAGAT TCCTGTCAGT 
CCCAACCAGC CGGCCTCTTT GGACGTCTCC TTGGAATCGT TGAACAAAAT GAGCCACAGC 
AGCTCCAGCT GCCTGTGCGC GGACGCCAGC CACCGAGCCG GCTGCCCCGT AGAGACGGGG 
AGGACCGCAG AGAGCTGCTG CAAGGTCAGC CAGTCCTCCT GCTCCTCCAG CTTCATCAGC 
AGGCCCGGGG TGGACCCCGC GGGCTCCCTT AGCGACTCCC TCTACGACAG TTTCTCCTCC 
TGCACCAGTC AGGGCTCCAA CGATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101162908 ( XM_020708329.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020563988.1 Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X4, mRNA.

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CloneID	OJa74625
Clone ID Related Accession (Same CDS sequence)	XM_020708329.1 , XM_020708329.2
Accession Version	XM_020708329.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	nck-associated protein 5-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGT TTACAGATCA CAGTGTCGCG TCGGTCTGCA GGAGGATCCG AAACATTCCC 
CTGCTGGAGC TGCTGGCGGA GTACATGGAC GCCAACAAAT GCATCGATGA GCTTCTGAAA 
CAGCTTGAGG AGGAGCGCCG GAATGTCCGA AGGGAAAAGC TGGCTGTGGC TCGCCTGCAG 
AGGGAAGTCG CACGAAGCAA GAGTGAAGGA ACAATGCGAG AAAAGTTGAT CCACGAGCTA 
GAGGAAGAAC GACGCCTGCG ACTGGAGAGT GAGAAGCGTC TCCGGGAAGT GACGGAGGAG 
TCAGAGCTGG GAAGGGCGCA GATGATTTCA CTGCAGCAGC AGTTCTCCAG GATGGAAGAG 
ACGGTGCGGT CGCTGCTGCA GAACCAGGGC GTCCTGGAGC AGACTGCTGT GGACACAGTG 
GACATCATGA AGGCCTACAA GGATAAACTC TCCGAGGAAG TGCAGAAACT GCAGGATGGC 
CCAGAGGCGA AGCAGGCCGA GCCAGCGAGT ACCGAGCTCG ACGGCAGCCA AGCGGACGAA 
GACAGAGACA AAACCAAGCT CCTTCTGGAG CGCCTGAAGA CCCTCGAGGA GGAGAACTCG 
GCGTTGGCCT CGGAGAACGA GAGCCAGAGG GAACAGTACG AGCGTTGCCT GGACGAGGTC 
GCTAACCAAG TTGTACAGGC ACTCCTCACA CAGAAGGATC TGAGAGAGGA ATGTCTTAAG 
CTGCGGACAA GAGTTTTTGA TCTGGAGCAG CAGAATCGAG CACTGAGTGT TTTGTTCCAG 
CAGAAGATAA AGCCGGCATC AGACCTCCTC CTTCAGAAAC TCCACTCGCG GATCATGGAT 
CTGTCTGCGG CAGATTTGCT TCTGGAGCCG GAGAGGAGCA AGGCCTTCCT CCTTTCCAGG 
AATACAGACT CCCCTTCCAA CGAGGTTCAT TCGAATGGAA AGCCAGGTGT CCCTGTGGCC 
AAATGTCTGA GCCAGATGAG TCTGATGGCG CCCGCTCCCA TCTATTCTCG CAGCAGCAGC 
GAGCTCTCAC TGTCGAGCGC GTGCAGCGAG TTCTCCAGCG GCTCCTACAC CTGGAATGAT 
GGGCGATCCT GCGGGAAAAT GTCATCCCTG ACGTGGGAGA AGAGACTCAG TTTGGGCTCG 
TCAGCTCCAA GTAACATCTG CGCGCCGCTG GAGGAACAGC CCCCCACACG GCGTAAGGAG 
AGCCACATAC TGGAGGGCCT GAGAAAACTC CAGCGGAGGA AACACAGGAG CTCCTCCTGC 
TCCTCTAGAG TGTCCAAGTC AGGCTACAAA GATTGCATGA ACTCCAACGA GGGCATCTAC 
TCTTTGGGTA TCAAGAGCAG CAGCAAAGGG GTGTCTAAAC CTGTACATGT CAGCAGAGCT 
TCTGCTGCTC TTGGAAGGCG GTTTTCCTAC GATTCCGATG ATGCTGACGA TGAACTGGCA 
CGCTCTAGTT GCAGAGATAA TATTCCCACT AAGGAGAGCT GGATTCACTG CAAGAAGCTC 
TCCCACAGCA TCTCTGATAG TTTGTGCAGC TTGGAGGGGA CACAGGAGGC TGTGGGTGAT 
GGTACTTCTT GTCCCACAGC TTCAAAACCC TATGACTCAA GGGAGCGTCC GGAGAAGCTC 
ATGAGTTTCA TCAACAGTTT TCTTCCTTTA GAAGGGCGTA AATCAGCCTT TAGCAGGCCA 
ACCGTGCACC ACAGAAATCC TACTCAAGTA GAAGATCCGA GTTGCAGATC CAACGCAGAT 
GATCCAGAGG AAACCATTCC TGATTCTGTG GGGGTCAAGA CGCCATGCAG TCAGCCGCAA 
GAACTAGCAG AGAGAGATAT CAAGAGGCTC TCAAGGGATA CCATCAAGAT TTTCAAGCAA 
CAGTTCATGC AAAAAGATCA GGGGCGCACT CAGTCTGCGG ATGGGAGACC GAAGCCTTTC 
AGCCTATTGA AGGAACTTAA AGCAACCAAG TGCACCCAGT CTGAGGAGAG CATACTGGCG 
ATATTTGATG CTGAAGGAGA ACCTATTGAT CTGTGCGCTC AGAGACTAGT ATCAGGAGCA 
GAAGGTCGCA AAGCCGCTAG CTGTTGCTCA GAGTTCCAAA AGAGGACAAC AAGGCAAAAT 
TCAGCAAACA CGACAAACTA TAGCGTTCTT GAATCTCCAG AAAAACCTTC AGAACATCAG 
ACTAGATCAG GCAAAGGCAG TGATAGCATG GAAGGAAGTG CAGAAAGGCT ACAGTTAGCT 
CCTCAGAGGA AGTTGATAAA GCCACCAAGC AGTCGAACTA ATAAAGGCTT TTCCATTCCT 
CCCATGAGTG ACGTCAACAG CCCCAAATCT GGTGGCTCTA AGATCCCCGG TGTCAATAAG 
CCCTTAGCTT CTCCGTTAAG AGTATCCAAG GGCACAACCA CTGAGCCTAA TAACAGTGGA 
AACTCAGGAC CCTCTGCTCA GGAGAAATCT TCGTCTTCTC TTACGGTTAA GATGTCTAGA 
TTCATCAAGA CGCAGGGAAC TTCCACTCAG AGTCCGAAAG CTGTGAACTG CAAGGTGTCT 
GGTCAGGCCG ACTTAAGCAA GACCTCTTCT TCCACTTCCC CCCACCTTCC AAAAAGACGC 
CTCGACTTCT CTGACAACAG CGAACAGCCG ACCAGAGACA AACACTGTGA AACCAGCAAG 
AACAAACTCA GGTCTCCATC TCCTCCCCCT CCCCCAGGAC GCACCACCTC TTTACTGGTC 
AGACCAAATT ACGAAGCGTC GCCTCAAGCA CACAAATCAG GGGCTCAACC ATCCACGCCA 
ACCACTGTGA GGGGCCCTCC CCCAAGTTAC CACACCTCAG TTGCACCAAA TATGCAAGGC 
ACGCTCCCCA TCAAGGATAA AGACTGTTTA GACGTAGATG CAGGCTATGG GACTGCACTT 
GCACCTCAGA AGCTGGTGGA GAAAACCACC CAGCACTTTC AGAAGACCAC TGCCGTAACT 
CAGACTCCAA CTAAAGGCAC TTCCAAGCGA ATAAAAGACT ACCTCCCACC TGCAAGCTCA 
GGATGTGCCA GAGTACCTGA AATTGCACCT AAAAACTCGA AGAATGTCCC TCCTCCCTAC 
AGTGCTCTAA GAGTGCCCTC CCTTCAAAAC TCCTTTGCAA ACAAAAAGGT ACCTGCTCAA 
GAAAACACCC ATCAGTCAGT GCAAAAGACT TCCATTAGTT TACCAATATC GCTTCAGGAT 
TCTGCTCAGT GTCAAACAGA GCCACAAAAA GCTGTCATGA GCCCTCCGGG CTCAGCTGTT 
ACGTCACCCA ATTTGTCAGA AAAAGCCTCA AAGACTCGAA TCCCAATGGG ATTTAAAGCC 
TTTCTAAAAT CCCCTCCCAG CCATAAAAGT AGTCCTACAA TAGCAGGCAA GCAAGAGAAA 
GATCATATCA ACTCTGTCTC CAAGGAAACT GTGACTTCAA ATGCTTCTAC TCAGTGTGAC 
ACCTCGCAGG CAGCGTACAG TACGGATTCA ACACCTACCG TGTCTGTTCC CGAAGGGAAA 
GGTGAATTTC TGTGTCAGCC ACAGAAAGAG GACTTACAAC CCCCCTTGTC GTCAGCAGAA 
GGAAATGTGA GTGACAAGGC AAAAAGGAGT AGTCAACTCT TTGGCAGATC CATATCTATT 
AATACCAAAC CACACCTAAA GCCAGCTTTA GGGATGAACG GGGCCAAAGC TCGAAGCCAG 
AGCTTCAGCA CCAACTACAT AGAGAAACCC AACATCAATG CCATGGAGGG GCCGGGAAGG 
ATCAGGACTC AAATCATCAC TAACTCAGGT GAAAGGGGGA ATTCGCTGTC AAGGCAGAGT 
TCTTCAGAAA CAACTGGGGT TGGATTGGCT GAAAGTCCAA TCCATGCCCC TAGAACCCGG 
CTAAGCCATC ATGGTGGCAT GATGGGACCA AATAGTCAGA GCGGCCTCCC TGAGAAGTCC 
ATTCAGTCGT GTGTTATAGG TGAAGGAAAA GCTTGTGGAG TGAAAGGAGA TGTGGTCACC 
TCTCCTTCTC AAAGAGAAGC TCGCAGCTTG CCCAAAAGTG ACAGAACTGC CTTTATTAAT 
GTCCAAAAGC CGGCAAAAGT GGACTCCCAT CCTCAAATCC AGTCTTCCTC AGCTTTTGTC 
TTTAGTCCTG AAAATCCTAC CAGAGAAGCA GAAACCATTA CCACCACTCC TGCTGAGCCA 
GACAAAACCC AGTTGGGTTC CCCAGGCAAT TTCCCAGACA CAGAGGAGAA AAAAATCAGC 
CCAACAACGT GCACTATTGA AGAGAAAGTG ATGATGGGGA TAGAAGAAAA CCTGCAGAAA 
AGTCAAGAGC AGGAGAAGGC TGCAGCTAAT GAGGCCAAAC AGAAGACGGG CCCGTCTTTG 
GCCAACTGGT TTGGCCTCCG CAAGAGCAAA CTACCAGCTT TTAGCGGCAA AAAAGCTGAT 
TCCCCGAAGG CAAGAGACGA GAAGAAAGAG CTGAAGATTG GGTCTGTGAT CAGCGGCAAG 
CAGATGAAGT CTGATAAGAA GAAGGAAAGG AAGAAGGATG GTCAGGAGGT CCAGAATTTG 
TCAGAGATGA ACAACAAGCT GAGCTCCATC ATGGACCACT GTAACAATCA GATGGGTCAG 
ATCGCCAGTC AGATCCAGAG CACCACTGTG TTAATCGGCA AGGACCAGTT TGTAAAAGAG 
CTTCTTGGGA GAGCTACTGT GAAGGGCAAC GCTTCGCCAC CTGGCATCTC TACACCGAAG 
AAACACTGTG AAACAAAGGG CGACATGCAG ATCTGTCCAG ATAATACCCT CATACTGACG 
CAAAAGATCA GCCTCAAGGC CAAAAACGAC GAGGGGAACA TTCCGGACAC GTCTTGTCAA 
GACCACATGA TAGATTCCCT TTTCACTCAC AATCCCTTCC GCTGCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020563988.1
CDS	126..4976
Translation	MSQFTDHSVASVCRRIRNIPLLELLAEYMDANKCIDELLKQLEEERRNVRREKLAVARLQREVARSKSEGTMREKLIHELEEERRLRLESEKRLREVTEESELGRAQMISLQQQFSRMEETVRSLLQNQGVLEQTAVDTVDIMKAYKDKLSEEVQKLQDGPEAKQAEPASTELDGSQADEDRDKTKLLLERLKTLEEENSALASENESQREQYERCLDEVANQVVQALLTQKDLREECLKLRTRVFDLEQQNRALSVLFQQKIKPASDLLLQKLHSRIMDLSAADLLLEPERSKAFLLSRNTDSPSNEVHSNGKPGVPVAKCLSQMSLMAPAPIYSRSSSELSLSSACSEFSSGSYTWNDGRSCGKMSSLTWEKRLSLGSSAPSNICAPLEEQPPTRRKESHILEGLRKLQRRKHRSSSCSSRVSKSGYKDCMNSNEGIYSLGIKSSSKGVSKPVHVSRASAALGRRFSYDSDDADDELARSSCRDNIPTKESWIHCKKLSHSISDSLCSLEGTQEAVGDGTSCPTASKPYDSRERPEKLMSFINSFLPLEGRKSAFSRPTVHHRNPTQVEDPSCRSNADDPEETIPDSVGVKTPCSQPQELAERDIKRLSRDTIKIFKQQFMQKDQGRTQSADGRPKPFSLLKELKATKCTQSEESILAIFDAEGEPIDLCAQRLVSGAEGRKAASCCSEFQKRTTRQNSANTTNYSVLESPEKPSEHQTRSGKGSDSMEGSAERLQLAPQRKLIKPPSSRTNKGFSIPPMSDVNSPKSGGSKIPGVNKPLASPLRVSKGTTTEPNNSGNSGPSAQEKSSSSLTVKMSRFIKTQGTSTQSPKAVNCKVSGQADLSKTSSSTSPHLPKRRLDFSDNSEQPTRDKHCETSKNKLRSPSPPPPPGRTTSLLVRPNYEASPQAHKSGAQPSTPTTVRGPPPSYHTSVAPNMQGTLPIKDKDCLDVDAGYGTALAPQKLVEKTTQHFQKTTAVTQTPTKGTSKRIKDYLPPASSGCARVPEIAPKNSKNVPPPYSALRVPSLQNSFANKKVPAQENTHQSVQKTSISLPISLQDSAQCQTEPQKAVMSPPGSAVTSPNLSEKASKTRIPMGFKAFLKSPPSHKSSPTIAGKQEKDHINSVSKETVTSNASTQCDTSQAAYSTDSTPTVSVPEGKGEFLCQPQKEDLQPPLSSAEGNVSDKAKRSSQLFGRSISINTKPHLKPALGMNGAKARSQSFSTNYIEKPNINAMEGPGRIRTQIITNSGERGNSLSRQSSSETTGVGLAESPIHAPRTRLSHHGGMMGPNSQSGLPEKSIQSCVIGEGKACGVKGDVVTSPSQREARSLPKSDRTAFINVQKPAKVDSHPQIQSSSAFVFSPENPTREAETITTTPAEPDKTQLGSPGNFPDTEEKKISPTTCTIEEKVMMGIEENLQKSQEQEKAAANEAKQKTGPSLANWFGLRKSKLPAFSGKKADSPKARDEKKELKIGSVISGKQMKSDKKKERKKDGQEVQNLSEMNNKLSSIMDHCNNQMGQIASQIQSTTVLIGKDQFVKELLGRATVKGNASPPGISTPKKHCETKGDMQICPDNTLILTQKISLKAKNDEGNIPDTSCQDHMIDSLFTHNPFRCM

Target ORF information:

RefSeq Version	XM_020708329.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nck-associated protein 5-like (LOC101162908), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020708329.1

ORF Insert Sequence:

ATGAGCCAGT TTACAGATCA CAGTGTCGCG TCGGTCTGCA GGAGGATCCG AAACATTCCC 
CTGCTGGAGC TGCTGGCGGA GTACATGGAC GCCAACAAAT GCATCGATGA GCTTCTGAAA 
CAGCTTGAGG AGGAGCGCCG GAATGTCCGA AGGGAAAAGC TGGCTGTGGC TCGCCTGCAG 
AGGGAAGTCG CACGAAGCAA GAGTGAAGGA ACAATGCGAG AAAAGTTGAT CCACGAGCTA 
GAGGAAGAAC GACGCCTGCG ACTGGAGAGT GAGAAGCGTC TCCGGGAAGT GACGGAGGAG 
TCAGAGCTGG GAAGGGCGCA GATGATTTCA CTGCAGCAGC AGTTCTCCAG GATGGAAGAG 
ACGGTGCGGT CGCTGCTGCA GAACCAGGGC GTCCTGGAGC AGACTGCTGT GGACACAGTG 
GACATCATGA AGGCCTACAA GGATAAACTC TCCGAGGAAG TGCAGAAACT GCAGGATGGC 
CCAGAGGCGA AGCAGGCCGA GCCAGCGAGT ACCGAGCTCG ACGGCAGCCA AGCGGACGAA 
GACAGAGACA AAACCAAGCT CCTTCTGGAG CGCCTGAAGA CCCTCGAGGA GGAGAACTCG 
GCGTTGGCCT CGGAGAACGA GAGCCAGAGG GAACAGTACG AGCGTTGCCT GGACGAGGTC 
GCTAACCAAG TTGTACAGGC ACTCCTCACA CAGAAGGATC TGAGAGAGGA ATGTCTTAAG 
CTGCGGACAA GAGTTTTTGA TCTGGAGCAG CAGAATCGAG CACTGAGTGT TTTGTTCCAG 
CAGAAGATAA AGCCGGCATC AGACCTCCTC CTTCAGAAAC TCCACTCGCG GATCATGGAT 
CTGTCTGCGG CAGATTTGCT TCTGGAGCCG GAGAGGAGCA AGGCCTTCCT CCTTTCCAGG 
AATACAGACT CCCCTTCCAA CGAGGTTCAT TCGAATGGAA AGCCAGGTGT CCCTGTGGCC 
AAATGTCTGA GCCAGATGAG TCTGATGGCG CCCGCTCCCA TCTATTCTCG CAGCAGCAGC 
GAGCTCTCAC TGTCGAGCGC GTGCAGCGAG TTCTCCAGCG GCTCCTACAC CTGGAATGAT 
GGGCGATCCT GCGGGAAAAT GTCATCCCTG ACGTGGGAGA AGAGACTCAG TTTGGGCTCG 
TCAGCTCCAA GTAACATCTG CGCGCCGCTG GAGGAACAGC CCCCCACACG GCGTAAGGAG 
AGCCACATAC TGGAGGGCCT GAGAAAACTC CAGCGGAGGA AACACAGGAG CTCCTCCTGC 
TCCTCTAGAG TGTCCAAGTC AGGCTACAAA GATTGCATGA ACTCCAACGA GGGCATCTAC 
TCTTTGGGTA TCAAGAGCAG CAGCAAAGGG GTGTCTAAAC CTGTACATGT CAGCAGAGCT 
TCTGCTGCTC TTGGAAGGCG GTTTTCCTAC GATTCCGATG ATGCTGACGA TGAACTGGCA 
CGCTCTAGTT GCAGAGATAA TATTCCCACT AAGGAGAGCT GGATTCACTG CAAGAAGCTC 
TCCCACAGCA TCTCTGATAG TTTGTGCAGC TTGGAGGGGA CACAGGAGGC TGTGGGTGAT 
GGTACTTCTT GTCCCACAGC TTCAAAACCC TATGACTCAA GGGAGCGTCC GGAGAAGCTC 
ATGAGTTTCA TCAACAGTTT TCTTCCTTTA GAAGGGCGTA AATCAGCCTT TAGCAGGCCA 
ACCGTGCACC ACAGAAATCC TACTCAAGTA GAAGATCCGA GTTGCAGATC CAACGCAGAT 
GATCCAGAGG AAACCATTCC TGATTCTGTG GGGGTCAAGA CGCCATGCAG TCAGCCGCAA 
GAACTAGCAG AGAGAGATAT CAAGAGGCTC TCAAGGGATA CCATCAAGAT TTTCAAGCAA 
CAGTTCATGC AAAAAGATCA GGGGCGCACT CAGTCTGCGG ATGGGAGACC GAAGCCTTTC 
AGCCTATTGA AGGAACTTAA AGCAACCAAG TGCACCCAGT CTGAGGAGAG CATACTGGCG 
ATATTTGATG CTGAAGGAGA ACCTATTGAT CTGTGCGCTC AGAGACTAGT ATCAGGAGCA 
GAAGGTCGCA AAGCCGCTAG CTGTTGCTCA GAGTTCCAAA AGAGGACAAC AAGGCAAAAT 
TCAGCAAACA CGACAAACTA TAGCGTTCTT GAATCTCCAG AAAAACCTTC AGAACATCAG 
ACTAGATCAG GCAAAGGCAG TGATAGCATG GAAGGAAGTG CAGAAAGGCT ACAGTTAGCT 
CCTCAGAGGA AGTTGATAAA GCCACCAAGC AGTCGAACTA ATAAAGGCTT TTCCATTCCT 
CCCATGAGTG ACGTCAACAG CCCCAAATCT GGTGGCTCTA AGATCCCCGG TGTCAATAAG 
CCCTTAGCTT CTCCGTTAAG AGTATCCAAG GGCACAACCA CTGAGCCTAA TAACAGTGGA 
AACTCAGGAC CCTCTGCTCA GGAGAAATCT TCGTCTTCTC TTACGGTTAA GATGTCTAGA 
TTCATCAAGA CGCAGGGAAC TTCCACTCAG AGTCCGAAAG CTGTGAACTG CAAGGTGTCT 
GGTCAGGCCG ACTTAAGCAA GACCTCTTCT TCCACTTCCC CCCACCTTCC AAAAAGACGC 
CTCGACTTCT CTGACAACAG CGAACAGCCG ACCAGAGACA AACACTGTGA AACCAGCAAG 
AACAAACTCA GGTCTCCATC TCCTCCCCCT CCCCCAGGAC GCACCACCTC TTTACTGGTC 
AGACCAAATT ACGAAGCGTC GCCTCAAGCA CACAAATCAG GGGCTCAACC ATCCACGCCA 
ACCACTGTGA GGGGCCCTCC CCCAAGTTAC CACACCTCAG TTGCACCAAA TATGCAAGGC 
ACGCTCCCCA TCAAGGATAA AGACTGTTTA GACGTAGATG CAGGCTATGG GACTGCACTT 
GCACCTCAGA AGCTGGTGGA GAAAACCACC CAGCACTTTC AGAAGACCAC TGCCGTAACT 
CAGACTCCAA CTAAAGGCAC TTCCAAGCGA ATAAAAGACT ACCTCCCACC TGCAAGCTCA 
GGATGTGCCA GAGTACCTGA AATTGCACCT AAAAACTCGA AGAATGTCCC TCCTCCCTAC 
AGTGCTCTAA GAGTGCCCTC CCTTCAAAAC TCCTTTGCAA ACAAAAAGGT ACCTGCTCAA 
GAAAACACCC ATCAGTCAGT GCAAAAGACT TCCATTAGTT TACCAATATC GCTTCAGGAT 
TCTGCTCAGT GTCAAACAGA GCCACAAAAA GCTGTCATGA GCCCTCCGGG CTCAGCTGTT 
ACGTCACCCA ATTTGTCAGA AAAAGCCTCA AAGACTCGAA TCCCAATGGG ATTTAAAGCC 
TTTCTAAAAT CCCCTCCCAG CCATAAAAGT AGTCCTACAA TAGCAGGCAA GCAAGAGAAA 
GATCATATCA ACTCTGTCTC CAAGGAAACT GTGACTTCAA ATGCTTCTAC TCAGTGTGAC 
ACCTCGCAGG CAGCGTACAG TACGGATTCA ACACCTACCG TGTCTGTTCC CGAAGGGAAA 
GGTGAATTTC TGTGTCAGCC ACAGAAAGAG GACTTACAAC CCCCCTTGTC GTCAGCAGAA 
GGAAATGTGA GTGACAAGGC AAAAAGGAGT AGTCAACTCT TTGGCAGATC CATATCTATT 
AATACCAAAC CACACCTAAA GCCAGCTTTA GGGATGAACG GGGCCAAAGC TCGAAGCCAG 
AGCTTCAGCA CCAACTACAT AGAGAAACCC AACATCAATG CCATGGAGGG GCCGGGAAGG 
ATCAGGACTC AAATCATCAC TAACTCAGGT GAAAGGGGGA ATTCGCTGTC AAGGCAGAGT 
TCTTCAGAAA CAACTGGGGT TGGATTGGCT GAAAGTCCAA TCCATGCCCC TAGAACCCGG 
CTAAGCCATC ATGGTGGCAT GATGGGACCA AATAGTCAGA GCGGCCTCCC TGAGAAGTCC 
ATTCAGTCGT GTGTTATAGG TGAAGGAAAA GCTTGTGGAG TGAAAGGAGA TGTGGTCACC 
TCTCCTTCTC AAAGAGAAGC TCGCAGCTTG CCCAAAAGTG ACAGAACTGC CTTTATTAAT 
GTCCAAAAGC CGGCAAAAGT GGACTCCCAT CCTCAAATCC AGTCTTCCTC AGCTTTTGTC 
TTTAGTCCTG AAAATCCTAC CAGAGAAGCA GAAACCATTA CCACCACTCC TGCTGAGCCA 
GACAAAACCC AGTTGGGTTC CCCAGGCAAT TTCCCAGACA CAGAGGAGAA AAAAATCAGC 
CCAACAACGT GCACTATTGA AGAGAAAGTG ATGATGGGGA TAGAAGAAAA CCTGCAGAAA 
AGTCAAGAGC AGGAGAAGGC TGCAGCTAAT GAGGCCAAAC AGAAGACGGG CCCGTCTTTG 
GCCAACTGGT TTGGCCTCCG CAAGAGCAAA CTACCAGCTT TTAGCGGCAA AAAAGCTGAT 
TCCCCGAAGG CAAGAGACGA GAAGAAAGAG CTGAAGATTG GGTCTGTGAT CAGCGGCAAG 
CAGATGAAGT CTGATAAGAA GAAGGAAAGG AAGAAGGATG GTCAGGAGGT CCAGAATTTG 
TCAGAGATGA ACAACAAGCT GAGCTCCATC ATGGACCACT GTAACAATCA GATGGGTCAG 
ATCGCCAGTC AGATCCAGAG CACCACTGTG TTAATCGGCA AGGACCAGTT TGTAAAAGAG 
CTTCTTGGGA GAGCTACTGT GAAGGGCAAC GCTTCGCCAC CTGGCATCTC TACACCGAAG 
AAACACTGTG AAACAAAGGG CGACATGCAG ATCTGTCCAG ATAATACCCT CATACTGACG 
CAAAAGATCA GCCTCAAGGC CAAAAACGAC GAGGGGAACA TTCCGGACAC GTCTTGTCAA 
GACCACATGA TAGATTCCCT TTTCACTCAC AATCCCTTCC GCTGCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101162908 ( XM_020708329.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020563988.1 Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X4, mRNA.

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CloneID	OJa74625
Clone ID Related Accession (Same CDS sequence)	XM_020708329.1 , XM_020708329.2
Accession Version	XM_020708329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	nck-associated protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020708329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGT TTACAGATCA CAGTGTCGCG TCGGTCTGCA GGAGGATCCG AAACATTCCC 
CTGCTGGAGC TGCTGGCGGA GTACATGGAC GCCAACAAAT GCATCGATGA GCTTCTGAAA 
CAGCTTGAGG AGGAGCGCCG GAATGTCCGA AGGGAAAAGC TGGCTGTGGC TCGCCTGCAG 
AGGGAAGTCG CACGAAGCAA GAGTGAAGGA ACAATGCGAG AAAAGTTGAT CCACGAGCTA 
GAGGAAGAAC GACGCCTGCG ACTGGAGAGT GAGAAGCGTC TCCGGGAAGT GACGGAGGAG 
TCAGAGCTGG GAAGGGCGCA GATGATTTCA CTGCAGCAGC AGTTCTCCAG GATGGAAGAG 
ACGGTGCGGT CGCTGCTGCA GAACCAGGGC GTCCTGGAGC AGACTGCTGT GGACACAGTG 
GACATCATGA AGGCCTACAA GGATAAACTC TCCGAGGAAG TGCAGAAACT GCAGGATGGC 
CCAGAGGCGA AGCAGGCCGA GCCAGCGAGT ACCGAGCTCG ACGGCAGCCA AGCGGACGAA 
GACAGAGACA AAACCAAGCT CCTTCTGGAG CGCCTGAAGA CCCTCGAGGA GGAGAACTCG 
GCGTTGGCCT CGGAGAACGA GAGCCAGAGG GAACAGTACG AGCGTTGCCT GGACGAGGTC 
GCTAACCAAG TTGTACAGGC ACTCCTCACA CAGAAGGATC TGAGAGAGGA ATGTCTTAAG 
CTGCGGACAA GAGTTTTTGA TCTGGAGCAG CAGAATCGAG CACTGAGTGT TTTGTTCCAG 
CAGAAGATAA AGCCGGCATC AGACCTCCTC CTTCAGAAAC TCCACTCGCG GATCATGGAT 
CTGTCTGCGG CAGATTTGCT TCTGGAGCCG GAGAGGAGCA AGGCCTTCCT CCTTTCCAGG 
AATACAGACT CCCCTTCCAA CGAGGTTCAT TCGAATGGAA AGCCAGGTGT CCCTGTGGCC 
AAATGTCTGA GCCAGATGAG TCTGATGGCG CCCGCTCCCA TCTATTCTCG CAGCAGCAGC 
GAGCTCTCAC TGTCGAGCGC GTGCAGCGAG TTCTCCAGCG GCTCCTACAC CTGGAATGAT 
GGGCGATCCT GCGGGAAAAT GTCATCCCTG ACGTGGGAGA AGAGACTCAG TTTGGGCTCG 
TCAGCTCCAA GTAACATCTG CGCGCCGCTG GAGGAACAGC CCCCCACACG GCGTAAGGAG 
AGCCACATAC TGGAGGGCCT GAGAAAACTC CAGCGGAGGA AACACAGGAG CTCCTCCTGC 
TCCTCTAGAG TGTCCAAGTC AGGCTACAAA GATTGCATGA ACTCCAACGA GGGCATCTAC 
TCTTTGGGTA TCAAGAGCAG CAGCAAAGGG GTGTCTAAAC CTGTACATGT CAGCAGAGCT 
TCTGCTGCTC TTGGAAGGCG GTTTTCCTAC GATTCCGATG ATGCTGACGA TGAACTGGCA 
CGCTCTAGTT GCAGAGATAA TATTCCCACT AAGGAGAGCT GGATTCACTG CAAGAAGCTC 
TCCCACAGCA TCTCTGATAG TTTGTGCAGC TTGGAGGGGA CACAGGAGGC TGTGGGTGAT 
GGTACTTCTT GTCCCACAGC TTCAAAACCC TATGACTCAA GGGAGCGTCC GGAGAAGCTC 
ATGAGTTTCA TCAACAGTTT TCTTCCTTTA GAAGGGCGTA AATCAGCCTT TAGCAGGCCA 
ACCGTGCACC ACAGAAATCC TACTCAAGTA GAAGATCCGA GTTGCAGATC CAACGCAGAT 
GATCCAGAGG AAACCATTCC TGATTCTGTG GGGGTCAAGA CGCCATGCAG TCAGCCGCAA 
GAACTAGCAG AGAGAGATAT CAAGAGGCTC TCAAGGGATA CCATCAAGAT TTTCAAGCAA 
CAGTTCATGC AAAAAGATCA GGGGCGCACT CAGTCTGCGG ATGGGAGACC GAAGCCTTTC 
AGCCTATTGA AGGAACTTAA AGCAACCAAG TGCACCCAGT CTGAGGAGAG CATACTGGCG 
ATATTTGATG CTGAAGGAGA ACCTATTGAT CTGTGCGCTC AGAGACTAGT ATCAGGAGCA 
GAAGGTCGCA AAGCCGCTAG CTGTTGCTCA GAGTTCCAAA AGAGGACAAC AAGGCAAAAT 
TCAGCAAACA CGACAAACTA TAGCGTTCTT GAATCTCCAG AAAAACCTTC AGAACATCAG 
ACTAGATCAG GCAAAGGCAG TGATAGCATG GAAGGAAGTG CAGAAAGGCT ACAGTTAGCT 
CCTCAGAGGA AGTTGATAAA GCCACCAAGC AGTCGAACTA ATAAAGGCTT TTCCATTCCT 
CCCATGAGTG ACGTCAACAG CCCCAAATCT GGTGGCTCTA AGATCCCCGG TGTCAATAAG 
CCCTTAGCTT CTCCGTTAAG AGTATCCAAG GGCACAACCA CTGAGCCTAA TAACAGTGGA 
AACTCAGGAC CCTCTGCTCA GGAGAAATCT TCGTCTTCTC TTACGGTTAA GATGTCTAGA 
TTCATCAAGA CGCAGGGAAC TTCCACTCAG AGTCCGAAAG CTGTGAACTG CAAGGTGTCT 
GGTCAGGCCG ACTTAAGCAA GACCTCTTCT TCCACTTCCC CCCACCTTCC AAAAAGACGC 
CTCGACTTCT CTGACAACAG CGAACAGCCG ACCAGAGACA AACACTGTGA AACCAGCAAG 
AACAAACTCA GGTCTCCATC TCCTCCCCCT CCCCCAGGAC GCACCACCTC TTTACTGGTC 
AGACCAAATT ACGAAGCGTC GCCTCAAGCA CACAAATCAG GGGCTCAACC ATCCACGCCA 
ACCACTGTGA GGGGCCCTCC CCCAAGTTAC CACACCTCAG TTGCACCAAA TATGCAAGGC 
ACGCTCCCCA TCAAGGATAA AGACTGTTTA GACGTAGATG CAGGCTATGG GACTGCACTT 
GCACCTCAGA AGCTGGTGGA GAAAACCACC CAGCACTTTC AGAAGACCAC TGCCGTAACT 
CAGACTCCAA CTAAAGGCAC TTCCAAGCGA ATAAAAGACT ACCTCCCACC TGCAAGCTCA 
GGATGTGCCA GAGTACCTGA AATTGCACCT AAAAACTCGA AGAATGTCCC TCCTCCCTAC 
AGTGCTCTAA GAGTGCCCTC CCTTCAAAAC TCCTTTGCAA ACAAAAAGGT ACCTGCTCAA 
GAAAACACCC ATCAGTCAGT GCAAAAGACT TCCATTAGTT TACCAATATC GCTTCAGGAT 
TCTGCTCAGT GTCAAACAGA GCCACAAAAA GCTGTCATGA GCCCTCCGGG CTCAGCTGTT 
ACGTCACCCA ATTTGTCAGA AAAAGCCTCA AAGACTCGAA TCCCAATGGG ATTTAAAGCC 
TTTCTAAAAT CCCCTCCCAG CCATAAAAGT AGTCCTACAA TAGCAGGCAA GCAAGAGAAA 
GATCATATCA ACTCTGTCTC CAAGGAAACT GTGACTTCAA ATGCTTCTAC TCAGTGTGAC 
ACCTCGCAGG CAGCGTACAG TACGGATTCA ACACCTACCG TGTCTGTTCC CGAAGGGAAA 
GGTGAATTTC TGTGTCAGCC ACAGAAAGAG GACTTACAAC CCCCCTTGTC GTCAGCAGAA 
GGAAATGTGA GTGACAAGGC AAAAAGGAGT AGTCAACTCT TTGGCAGATC CATATCTATT 
AATACCAAAC CACACCTAAA GCCAGCTTTA GGGATGAACG GGGCCAAAGC TCGAAGCCAG 
AGCTTCAGCA CCAACTACAT AGAGAAACCC AACATCAATG CCATGGAGGG GCCGGGAAGG 
ATCAGGACTC AAATCATCAC TAACTCAGGT GAAAGGGGGA ATTCGCTGTC AAGGCAGAGT 
TCTTCAGAAA CAACTGGGGT TGGATTGGCT GAAAGTCCAA TCCATGCCCC TAGAACCCGG 
CTAAGCCATC ATGGTGGCAT GATGGGACCA AATAGTCAGA GCGGCCTCCC TGAGAAGTCC 
ATTCAGTCGT GTGTTATAGG TGAAGGAAAA GCTTGTGGAG TGAAAGGAGA TGTGGTCACC 
TCTCCTTCTC AAAGAGAAGC TCGCAGCTTG CCCAAAAGTG ACAGAACTGC CTTTATTAAT 
GTCCAAAAGC CGGCAAAAGT GGACTCCCAT CCTCAAATCC AGTCTTCCTC AGCTTTTGTC 
TTTAGTCCTG AAAATCCTAC CAGAGAAGCA GAAACCATTA CCACCACTCC TGCTGAGCCA 
GACAAAACCC AGTTGGGTTC CCCAGGCAAT TTCCCAGACA CAGAGGAGAA AAAAATCAGC 
CCAACAACGT GCACTATTGA AGAGAAAGTG ATGATGGGGA TAGAAGAAAA CCTGCAGAAA 
AGTCAAGAGC AGGAGAAGGC TGCAGCTAAT GAGGCCAAAC AGAAGACGGG CCCGTCTTTG 
GCCAACTGGT TTGGCCTCCG CAAGAGCAAA CTACCAGCTT TTAGCGGCAA AAAAGCTGAT 
TCCCCGAAGG CAAGAGACGA GAAGAAAGAG CTGAAGATTG GGTCTGTGAT CAGCGGCAAG 
CAGATGAAGT CTGATAAGAA GAAGGAAAGG AAGAAGGATG GTCAGGAGGT CCAGAATTTG 
TCAGAGATGA ACAACAAGCT GAGCTCCATC ATGGACCACT GTAACAATCA GATGGGTCAG 
ATCGCCAGTC AGATCCAGAG CACCACTGTG TTAATCGGCA AGGACCAGTT TGTAAAAGAG 
CTTCTTGGGA GAGCTACTGT GAAGGGCAAC GCTTCGCCAC CTGGCATCTC TACACCGAAG 
AAACACTGTG AAACAAAGGG CGACATGCAG ATCTGTCCAG ATAATACCCT CATACTGACG 
CAAAAGATCA GCCTCAAGGC CAAAAACGAC GAGGGGAACA TTCCGGACAC GTCTTGTCAA 
GACCACATGA TAGATTCCCT TTTCACTCAC AATCCCTTCC GCTGCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020563988.1
CDS	160..5010
Translation	MSQFTDHSVASVCRRIRNIPLLELLAEYMDANKCIDELLKQLEEERRNVRREKLAVARLQREVARSKSEGTMREKLIHELEEERRLRLESEKRLREVTEESELGRAQMISLQQQFSRMEETVRSLLQNQGVLEQTAVDTVDIMKAYKDKLSEEVQKLQDGPEAKQAEPASTELDGSQADEDRDKTKLLLERLKTLEEENSALASENESQREQYERCLDEVANQVVQALLTQKDLREECLKLRTRVFDLEQQNRALSVLFQQKIKPASDLLLQKLHSRIMDLSAADLLLEPERSKAFLLSRNTDSPSNEVHSNGKPGVPVAKCLSQMSLMAPAPIYSRSSSELSLSSACSEFSSGSYTWNDGRSCGKMSSLTWEKRLSLGSSAPSNICAPLEEQPPTRRKESHILEGLRKLQRRKHRSSSCSSRVSKSGYKDCMNSNEGIYSLGIKSSSKGVSKPVHVSRASAALGRRFSYDSDDADDELARSSCRDNIPTKESWIHCKKLSHSISDSLCSLEGTQEAVGDGTSCPTASKPYDSRERPEKLMSFINSFLPLEGRKSAFSRPTVHHRNPTQVEDPSCRSNADDPEETIPDSVGVKTPCSQPQELAERDIKRLSRDTIKIFKQQFMQKDQGRTQSADGRPKPFSLLKELKATKCTQSEESILAIFDAEGEPIDLCAQRLVSGAEGRKAASCCSEFQKRTTRQNSANTTNYSVLESPEKPSEHQTRSGKGSDSMEGSAERLQLAPQRKLIKPPSSRTNKGFSIPPMSDVNSPKSGGSKIPGVNKPLASPLRVSKGTTTEPNNSGNSGPSAQEKSSSSLTVKMSRFIKTQGTSTQSPKAVNCKVSGQADLSKTSSSTSPHLPKRRLDFSDNSEQPTRDKHCETSKNKLRSPSPPPPPGRTTSLLVRPNYEASPQAHKSGAQPSTPTTVRGPPPSYHTSVAPNMQGTLPIKDKDCLDVDAGYGTALAPQKLVEKTTQHFQKTTAVTQTPTKGTSKRIKDYLPPASSGCARVPEIAPKNSKNVPPPYSALRVPSLQNSFANKKVPAQENTHQSVQKTSISLPISLQDSAQCQTEPQKAVMSPPGSAVTSPNLSEKASKTRIPMGFKAFLKSPPSHKSSPTIAGKQEKDHINSVSKETVTSNASTQCDTSQAAYSTDSTPTVSVPEGKGEFLCQPQKEDLQPPLSSAEGNVSDKAKRSSQLFGRSISINTKPHLKPALGMNGAKARSQSFSTNYIEKPNINAMEGPGRIRTQIITNSGERGNSLSRQSSSETTGVGLAESPIHAPRTRLSHHGGMMGPNSQSGLPEKSIQSCVIGEGKACGVKGDVVTSPSQREARSLPKSDRTAFINVQKPAKVDSHPQIQSSSAFVFSPENPTREAETITTTPAEPDKTQLGSPGNFPDTEEKKISPTTCTIEEKVMMGIEENLQKSQEQEKAAANEAKQKTGPSLANWFGLRKSKLPAFSGKKADSPKARDEKKELKIGSVISGKQMKSDKKKERKKDGQEVQNLSEMNNKLSSIMDHCNNQMGQIASQIQSTTVLIGKDQFVKELLGRATVKGNASPPGISTPKKHCETKGDMQICPDNTLILTQKISLKAKNDEGNIPDTSCQDHMIDSLFTHNPFRCM

Target ORF information:

RefSeq Version	XM_020708329.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nck-associated protein 5 (LOC101162908), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020708329.2

ORF Insert Sequence:

ATGAGCCAGT TTACAGATCA CAGTGTCGCG TCGGTCTGCA GGAGGATCCG AAACATTCCC 
CTGCTGGAGC TGCTGGCGGA GTACATGGAC GCCAACAAAT GCATCGATGA GCTTCTGAAA 
CAGCTTGAGG AGGAGCGCCG GAATGTCCGA AGGGAAAAGC TGGCTGTGGC TCGCCTGCAG 
AGGGAAGTCG CACGAAGCAA GAGTGAAGGA ACAATGCGAG AAAAGTTGAT CCACGAGCTA 
GAGGAAGAAC GACGCCTGCG ACTGGAGAGT GAGAAGCGTC TCCGGGAAGT GACGGAGGAG 
TCAGAGCTGG GAAGGGCGCA GATGATTTCA CTGCAGCAGC AGTTCTCCAG GATGGAAGAG 
ACGGTGCGGT CGCTGCTGCA GAACCAGGGC GTCCTGGAGC AGACTGCTGT GGACACAGTG 
GACATCATGA AGGCCTACAA GGATAAACTC TCCGAGGAAG TGCAGAAACT GCAGGATGGC 
CCAGAGGCGA AGCAGGCCGA GCCAGCGAGT ACCGAGCTCG ACGGCAGCCA AGCGGACGAA 
GACAGAGACA AAACCAAGCT CCTTCTGGAG CGCCTGAAGA CCCTCGAGGA GGAGAACTCG 
GCGTTGGCCT CGGAGAACGA GAGCCAGAGG GAACAGTACG AGCGTTGCCT GGACGAGGTC 
GCTAACCAAG TTGTACAGGC ACTCCTCACA CAGAAGGATC TGAGAGAGGA ATGTCTTAAG 
CTGCGGACAA GAGTTTTTGA TCTGGAGCAG CAGAATCGAG CACTGAGTGT TTTGTTCCAG 
CAGAAGATAA AGCCGGCATC AGACCTCCTC CTTCAGAAAC TCCACTCGCG GATCATGGAT 
CTGTCTGCGG CAGATTTGCT TCTGGAGCCG GAGAGGAGCA AGGCCTTCCT CCTTTCCAGG 
AATACAGACT CCCCTTCCAA CGAGGTTCAT TCGAATGGAA AGCCAGGTGT CCCTGTGGCC 
AAATGTCTGA GCCAGATGAG TCTGATGGCG CCCGCTCCCA TCTATTCTCG CAGCAGCAGC 
GAGCTCTCAC TGTCGAGCGC GTGCAGCGAG TTCTCCAGCG GCTCCTACAC CTGGAATGAT 
GGGCGATCCT GCGGGAAAAT GTCATCCCTG ACGTGGGAGA AGAGACTCAG TTTGGGCTCG 
TCAGCTCCAA GTAACATCTG CGCGCCGCTG GAGGAACAGC CCCCCACACG GCGTAAGGAG 
AGCCACATAC TGGAGGGCCT GAGAAAACTC CAGCGGAGGA AACACAGGAG CTCCTCCTGC 
TCCTCTAGAG TGTCCAAGTC AGGCTACAAA GATTGCATGA ACTCCAACGA GGGCATCTAC 
TCTTTGGGTA TCAAGAGCAG CAGCAAAGGG GTGTCTAAAC CTGTACATGT CAGCAGAGCT 
TCTGCTGCTC TTGGAAGGCG GTTTTCCTAC GATTCCGATG ATGCTGACGA TGAACTGGCA 
CGCTCTAGTT GCAGAGATAA TATTCCCACT AAGGAGAGCT GGATTCACTG CAAGAAGCTC 
TCCCACAGCA TCTCTGATAG TTTGTGCAGC TTGGAGGGGA CACAGGAGGC TGTGGGTGAT 
GGTACTTCTT GTCCCACAGC TTCAAAACCC TATGACTCAA GGGAGCGTCC GGAGAAGCTC 
ATGAGTTTCA TCAACAGTTT TCTTCCTTTA GAAGGGCGTA AATCAGCCTT TAGCAGGCCA 
ACCGTGCACC ACAGAAATCC TACTCAAGTA GAAGATCCGA GTTGCAGATC CAACGCAGAT 
GATCCAGAGG AAACCATTCC TGATTCTGTG GGGGTCAAGA CGCCATGCAG TCAGCCGCAA 
GAACTAGCAG AGAGAGATAT CAAGAGGCTC TCAAGGGATA CCATCAAGAT TTTCAAGCAA 
CAGTTCATGC AAAAAGATCA GGGGCGCACT CAGTCTGCGG ATGGGAGACC GAAGCCTTTC 
AGCCTATTGA AGGAACTTAA AGCAACCAAG TGCACCCAGT CTGAGGAGAG CATACTGGCG 
ATATTTGATG CTGAAGGAGA ACCTATTGAT CTGTGCGCTC AGAGACTAGT ATCAGGAGCA 
GAAGGTCGCA AAGCCGCTAG CTGTTGCTCA GAGTTCCAAA AGAGGACAAC AAGGCAAAAT 
TCAGCAAACA CGACAAACTA TAGCGTTCTT GAATCTCCAG AAAAACCTTC AGAACATCAG 
ACTAGATCAG GCAAAGGCAG TGATAGCATG GAAGGAAGTG CAGAAAGGCT ACAGTTAGCT 
CCTCAGAGGA AGTTGATAAA GCCACCAAGC AGTCGAACTA ATAAAGGCTT TTCCATTCCT 
CCCATGAGTG ACGTCAACAG CCCCAAATCT GGTGGCTCTA AGATCCCCGG TGTCAATAAG 
CCCTTAGCTT CTCCGTTAAG AGTATCCAAG GGCACAACCA CTGAGCCTAA TAACAGTGGA 
AACTCAGGAC CCTCTGCTCA GGAGAAATCT TCGTCTTCTC TTACGGTTAA GATGTCTAGA 
TTCATCAAGA CGCAGGGAAC TTCCACTCAG AGTCCGAAAG CTGTGAACTG CAAGGTGTCT 
GGTCAGGCCG ACTTAAGCAA GACCTCTTCT TCCACTTCCC CCCACCTTCC AAAAAGACGC 
CTCGACTTCT CTGACAACAG CGAACAGCCG ACCAGAGACA AACACTGTGA AACCAGCAAG 
AACAAACTCA GGTCTCCATC TCCTCCCCCT CCCCCAGGAC GCACCACCTC TTTACTGGTC 
AGACCAAATT ACGAAGCGTC GCCTCAAGCA CACAAATCAG GGGCTCAACC ATCCACGCCA 
ACCACTGTGA GGGGCCCTCC CCCAAGTTAC CACACCTCAG TTGCACCAAA TATGCAAGGC 
ACGCTCCCCA TCAAGGATAA AGACTGTTTA GACGTAGATG CAGGCTATGG GACTGCACTT 
GCACCTCAGA AGCTGGTGGA GAAAACCACC CAGCACTTTC AGAAGACCAC TGCCGTAACT 
CAGACTCCAA CTAAAGGCAC TTCCAAGCGA ATAAAAGACT ACCTCCCACC TGCAAGCTCA 
GGATGTGCCA GAGTACCTGA AATTGCACCT AAAAACTCGA AGAATGTCCC TCCTCCCTAC 
AGTGCTCTAA GAGTGCCCTC CCTTCAAAAC TCCTTTGCAA ACAAAAAGGT ACCTGCTCAA 
GAAAACACCC ATCAGTCAGT GCAAAAGACT TCCATTAGTT TACCAATATC GCTTCAGGAT 
TCTGCTCAGT GTCAAACAGA GCCACAAAAA GCTGTCATGA GCCCTCCGGG CTCAGCTGTT 
ACGTCACCCA ATTTGTCAGA AAAAGCCTCA AAGACTCGAA TCCCAATGGG ATTTAAAGCC 
TTTCTAAAAT CCCCTCCCAG CCATAAAAGT AGTCCTACAA TAGCAGGCAA GCAAGAGAAA 
GATCATATCA ACTCTGTCTC CAAGGAAACT GTGACTTCAA ATGCTTCTAC TCAGTGTGAC 
ACCTCGCAGG CAGCGTACAG TACGGATTCA ACACCTACCG TGTCTGTTCC CGAAGGGAAA 
GGTGAATTTC TGTGTCAGCC ACAGAAAGAG GACTTACAAC CCCCCTTGTC GTCAGCAGAA 
GGAAATGTGA GTGACAAGGC AAAAAGGAGT AGTCAACTCT TTGGCAGATC CATATCTATT 
AATACCAAAC CACACCTAAA GCCAGCTTTA GGGATGAACG GGGCCAAAGC TCGAAGCCAG 
AGCTTCAGCA CCAACTACAT AGAGAAACCC AACATCAATG CCATGGAGGG GCCGGGAAGG 
ATCAGGACTC AAATCATCAC TAACTCAGGT GAAAGGGGGA ATTCGCTGTC AAGGCAGAGT 
TCTTCAGAAA CAACTGGGGT TGGATTGGCT GAAAGTCCAA TCCATGCCCC TAGAACCCGG 
CTAAGCCATC ATGGTGGCAT GATGGGACCA AATAGTCAGA GCGGCCTCCC TGAGAAGTCC 
ATTCAGTCGT GTGTTATAGG TGAAGGAAAA GCTTGTGGAG TGAAAGGAGA TGTGGTCACC 
TCTCCTTCTC AAAGAGAAGC TCGCAGCTTG CCCAAAAGTG ACAGAACTGC CTTTATTAAT 
GTCCAAAAGC CGGCAAAAGT GGACTCCCAT CCTCAAATCC AGTCTTCCTC AGCTTTTGTC 
TTTAGTCCTG AAAATCCTAC CAGAGAAGCA GAAACCATTA CCACCACTCC TGCTGAGCCA 
GACAAAACCC AGTTGGGTTC CCCAGGCAAT TTCCCAGACA CAGAGGAGAA AAAAATCAGC 
CCAACAACGT GCACTATTGA AGAGAAAGTG ATGATGGGGA TAGAAGAAAA CCTGCAGAAA 
AGTCAAGAGC AGGAGAAGGC TGCAGCTAAT GAGGCCAAAC AGAAGACGGG CCCGTCTTTG 
GCCAACTGGT TTGGCCTCCG CAAGAGCAAA CTACCAGCTT TTAGCGGCAA AAAAGCTGAT 
TCCCCGAAGG CAAGAGACGA GAAGAAAGAG CTGAAGATTG GGTCTGTGAT CAGCGGCAAG 
CAGATGAAGT CTGATAAGAA GAAGGAAAGG AAGAAGGATG GTCAGGAGGT CCAGAATTTG 
TCAGAGATGA ACAACAAGCT GAGCTCCATC ATGGACCACT GTAACAATCA GATGGGTCAG 
ATCGCCAGTC AGATCCAGAG CACCACTGTG TTAATCGGCA AGGACCAGTT TGTAAAAGAG 
CTTCTTGGGA GAGCTACTGT GAAGGGCAAC GCTTCGCCAC CTGGCATCTC TACACCGAAG 
AAACACTGTG AAACAAAGGG CGACATGCAG ATCTGTCCAG ATAATACCCT CATACTGACG 
CAAAAGATCA GCCTCAAGGC CAAAAACGAC GAGGGGAACA TTCCGGACAC GTCTTGTCAA 
GACCACATGA TAGATTCCCT TTTCACTCAC AATCCCTTCC GCTGCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101162908 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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