col12a1 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	col12a1
Entrez Gene ID	101158649
Full Name	collagen type XII alpha 1 chain
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011490504.3	XP_011488806.1	collagen alpha-1(XII) chain isoform X3
XM_011490502.3	XP_011488804.1	collagen alpha-1(XII) chain isoform X1
XM_011490503.3	XP_011488805.1	collagen alpha-1(XII) chain isoform X2
XM_011490504.2	XP_011488806.1	collagen alpha-1(XII) chain isoform X3
XM_011490502.2	XP_011488804.1	collagen alpha-1(XII) chain isoform X1
XM_011490503.2	XP_011488805.1	collagen alpha-1(XII) chain isoform X2

Pathways from BioSystems
Gene Ontology

The following col12a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col12a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa24261	XM_011490502.3 Latest version!	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OJa24262	XM_011490503.3 Latest version!	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OJa24263	XM_011490504.3 Latest version!	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OJa24263	XM_011490504.2	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OJa24261	XM_011490502.2	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OJa24262	XM_011490503.2	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col12a1 ( XM_011490502.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488804.1 Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X1, mRNA.

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CloneID	OJa24261
Clone ID Related Accession (Same CDS sequence)	XM_011490502.3 , XM_011490502.2
Accession Version	XM_011490502.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	collagen alpha-1(XII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011490502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TGTCCAAGTC AGATGGTAGA CCGATACCAA ATGCACCTCC CACCAATTTG 
GTTGTTTTCA ATGAGACCAC CACCACCCTG AATGCTAGGT GGACTCCACC TCTGGGTCGT 
GTCCAGAACT ACAGGATCAC TTATGTCCCC ACATCTGGAG GCCAGAGTCA GACTACCATG 
GTTGGAGCCA AGAAAAACAC AGTCCTGCTT CCAAAGCTGA CCCCTGACAC AGAGTACTCC 
ATCGGTGTTG TGGCGGTGTA CGCCAATGGA GTCAGCAGGG AGCTGAATGG CCTTGGAAAA 
ACCAAACCTT TGGGTGGTGT TAGAAACCTG CGTGTGACAA ACCCCACCAC GAGCACGTTG 
GATGTGAACT GGGAACCTGC TGAGGGTAAT GTGCGCCAGT ATAGGGTCTT CTATGTTCCT 
GCAGCTGGAG GAGAGCAGGA GATGATTCAG GTCTCCGGAA GCACCTACAA AGCAGTTCTG 
AAGAACCTGC AGTCGGACAC TGTCTACACT GTCACAGTGA CTCCTGTTTA TTCTACTGGA 
GAGGGGAAGC CCATGTCTGA AAACGGCAAA ACATTGGAGC GCAGTCCTGC TAGGAATATC 
CAGGTTTTCA ACCCCACAAC TAACACTTTG AATGTCCGCT GGGAAGCAGC CACCGGGCCG 
GTCCAGCAGT ACAGGGTGGT CTATGCTCCT TTGACTGGTG TGAGGCCAAC GGAGTCGATT 
TTGGTCCCCG GCACCACAAC CACAGCACTG CTGCAGCAGC TGGTCCCAGA CACAGCCTAC 
AATGTGGGAG TTGTTGCCTT GTACAGCGAT GGGGAAGGAA CCACTGTCAG CGATCAGGGA 
AAGACTTTGC CCCGGGGTGG TCCCAGAAAC ATGCGTGTGT ACGATCCCAC CACCAGTACG 
CTGTCTGTTA ACTGGGAACA CGCTGACGGT CCTGTTCTTC AGTACCGCAT CAGCTATGCC 
CCAACAACAG GAGACCCTAT CGAGGAATTT ACCACAGTTC AAGGGAACAG GAACAATGTG 
GTTCTTCAGA ATCTGGATCC AGACACTCCC TACAATATCA GAGTGACTGC TATGTATGCT 
GAAGGAGCGG GAGGAGAACT GGAGGGAGAT GGACGTACAG TTGGAATGCT CGGCCCCAGA 
AACCTGCGTG TATCTGATGA ATGGTACACT CGCTTCAGGG TGTCCTGGGA CCCCGCCCCC 
TCCGCAGTCA CTGGATACAA ACTCATCTAC CAGCCTGCGG GGTCTTATGA CTCATCTGAG 
GTGTTTGTTG GAGATGTGAC CACCTATCAG CTGCAAAACT TGAAGCCTGG AACTACCTAT 
GACTTGACGG TCCTAGCACA GTACAGAACG GGCATCAGTG GTCCTCTAAA TGGTCAAGGC 
ACCACATTGT ACCTCAATGT GACAGGCCTA ACCTCCTACA ATGTTGGCTA TGACTCCTTT 
TGCATCCAGT GGGTCCCTCA CCGAGCAGCC ACGTCGTACA GACTTAAAGT CAACCCTTTT 
GAGTCCTTCA AGGGTGGAGC TCAAGAAATC ACAGTTCAGG GCCCAGAGAG TAAATACTGC 
TTTGACAACC TGACCCCTGA CACTCTTTAC AACGCTACGG TTTACGCTCA GACCCTCAAC 
CTTGAAGGAC CTGGAGTCAG TATTAAGGAG AAAACATTGG TCAAACCCAC CGAGGTGCCA 
ACCGAGCCTC CCACCCCTCC CCCTCCATCA ATAGTGCCTG ATGCTCTTGA CATGTGTAAA 
GGTGCGAAGG CTGACTTGGT CTTCCTCATC GACGGCTCAT GGAGTATTGG AGATGACAGC 
TTTGCCAAAG TCCTCCAGTT TGTCACTAGT GTGACAGCAG CTTTTGATGT TGTTAGCCCA 
ACTGGAATGC AGGTTTCATT TGTGCAGTAT AGCGATGATG CTAAGACTGA GTTCAAACTG 
AACACCTACC ACAACAAGGG CATTGTCCTA TCTGCACTGA AGTCAATCCG CTACAGGGGA 
GGCAACACTA AGACTGGCAT TGCTCTGAAG CACGTCTATG AAAAGGTACT AACGTCCGAC 
AATGGCATGA GGAGGAATGT CCCGAAGGTG CTGGTTGTTG TCACAGATGG TCGTTCTCAA 
GATGAAGTCA AGAAGAGCGC AGAAAAACTT CAGCATTCTG GCTATAGTGT GTTTGTGGTA 
GGAGTGGCTG ATGTGAGCAT GAGAGAACTG GCAGTTATCG GCAGCAAGCC CACCGAAAGA 
CACGTGTTTG TCGTTGACGA TTACGACGCT TTTTCCAAAA TCCAGGACAA CTTGATCACA 
TTCATCTGTG AAACGGCTAC CTCCACTTGC CCTCTCATCT ACATCAATGG ATACACCACA 
CCTGGATTTA GGATGCTGGA AGCTTTCAAC ATCACAGACC GAACATTTGC TGGCATGAAT 
GGTGTCTCCA TGGAGCCAGG TTCTTTCAAC AGCTTCATTG CCTACAGGCT GCACAAAGAT 
GCCTTCCTAA ACCAGCCCAC AAAGGAAATC CACTCCGACG GCCTTCCTCC ATCCTACACT 
ATAATCCTGC TCCTCCGCCT GCTGCCTGAT ACACCCTCTG AACCTTTTGA TATCTGGCAG 
ATCTCTGACA AAAACAACAA CCCTGAAGTT GGAGTCACTA TGAATCCTTC CAGCAAAACA 
CTCACGTTCT ACAACAAGGA CACCCGGGGA GAGATCCAGA GAGCCGTGTT CAATGAGGAG 
CAAGTGAAAC GGGTTTTCCA CGGAAGCTTC CACAAGCTCC ACATTACCGT CTCCCCAGAG 
AAGGTCAAGC TCAACGTGGA CTGCCAAGAG GTGGCAGAGA AGCCAATCAA GGAGGCCAAC 
AACATCACGC TGGAGGGCTA TGAAGTGCTT GGCAAGATGG TCAAATCAGG AGGTGGAAAG 
AGGCAGTCCG CTACATTCCA GCTCCAGATG TTTGACATTG TCTGCAGCTT GAGTTGGATC 
AGCCGTGACA AATGCTGTGA CCTGCCTGCT ACGAGAATTG AGTCCAAATG TCCTGCTCTT 
CCCCACTCCT GCACATGCAC CCAAGACAGT GTTGGGCCCC AGGGGCCTCC TGGGCCTTCG 
GGCAGCCCAG GGTCTAAAGG ACCCAGAGGA GACAGGGGAG ACCCAGGCAA ACCTGGTGCA 
ATGGGTCCAC GAGGTGAGCC AGGACTCCAA GGTGCAATGG GCCCACCAGG CCCACAAGGC 
CCTAATGGAC TGTCTATCCC CGGAGAACCG GGTCGCCAAG GACCAAAGGG AGATCGTGGA 
GACTCAGGCC TGCCTGGGCT GCAAGGTCCT CCTGGGCCGC GTGGTCCTAT GGGTCCTGTC 
GGACAAAGTG GAGCACGGGG GCCACCAGGA AAGGAAGGAT CATCTGGACC CAGAGGCCCA 
CCAGGGCCCA TGGGAAGTCC AGGAAATCCA GGTGTACCTG GTATGTCTGG AAAACCAGGA 
AAGTCAGGAG AACCAGGAAG TCCAGGACCT GTTGGCCTTA AAGGAGAAAA GGGAGAGCGG 
GGAGACTTTG CATCCCAAAA TATGATGCGC TCCATTGCCA GACAAGTTTG TGAACAGCTC 
GTGAGCAGTC AGATGAGCAG AATTGACGGG ATGCTCAATC AAATTCCTTC TGGATATCGT 
AATGGCAATC CAGGCCCGGC TGGTCCTCCT GGTCCCCCTG GCAACCAAGG ACCTCGTGGA 
GAGCCCGGAC AACCTGGAAA ATCTGGTTTC CCTGGAAGCC CAGGATTACC TGGAAATCAA 
GGAGAAAGAG GGTTACCAGG AGAGAGGGGG GAGAGAGGAC CTCCAGGAGC AGATATCAGA 
GGACAAAGAG GTCCACCTGG ACCAGCAGGT CCACCAGGGG AGTCTAGAAC AGGTCCCCCA 
GGTTCTCCTG GCTCTGCTGG ATCCCGTGGC CCCCCAGGTC GTCAAGGTAA TCCAGGTGTG 
AGAGGACCAC CAGGACCCCC TGGATACTGT GACTCCTCTC AGTGTGTTGG TATTCCTTAC 
AATGGACAAG GATACACAGC TCCACGCTAC CAGCCTGAAC CTGATGTAGC GTCTATACCT 
GTGGAGCCAA CTGAAGAGTT TGAGACAGTA CAATCATCAG GTTATACAAG GAACCAACGG 
GGAAAGAGAT CGCTACCACT AGACAATTCA GGAGCATCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488804.1
CDS	230..9571
Translation	MKIRLSLATAAFLVGFLASIDAQVEPPSDLKFKILNENTVEMSWLKPSSRIEGFRIQVASDADEPSRDFTLDAYSTSTSITNLTPDVDYSVSISSYYGSEESISIFGQLTIQSSNTTRPRPTSDALKCSVSAIADLVFLVDGSWSVGRENFKHIRSFIGSLAGAFDIGEDKTRVAVVQYSTDTRTEFPLNRYTRRGDLLQAINSLPYKGGNTMTGDAIDYLIKNIFTEAAGSRKAFPKVAMIITDGKSQDPVEEHARRLRNIGVEIFVLGIKGADEDELREIASTPHAKHVYNVPNFDLIQEVQKRIITEVCSGVDDQLTSLVSGEEMVEAPSNLQVTEIASKSMRLTWDASLGDITGYKLTLIPLVPGMKRQELYMGATQTSINVRDLSPETEYDISLFALKGLTPSEPLTDLAKTQPVKVLTECSMDVDVQADVVLLVDGSYSIGLQNFAKVREFLEVLVKSFDIGPDKIQISLVQYSRDPHTEFYLNTHHDINAVVKAVRNFPYRGGSTNTGKAMTYVREKIFVPTRGARQNVPRVMVLITDGKSSDSFRVAATKLRNIDVEIFAVGVKDAVRSELEAIANEPADTHVYEVEDFDAFQRISKELTQSICLRIEQELLKIKKKNLLPPSNLQFSEITSRSFRATWVAPAASVMSYLVRYRKAEDITGDYISLAVPGQTTTTVLLYLTPITTYEVNVYAEYDKGDSFALTGQETTLEEQGTVENLRVTEETTNSFRVSWQAAPGSVIRYRLSYVPLSGMGEILEAQTLGPETTIVLQELFPITTYRVSVSPEYAAGVGDELQVDGTTKEVIGSPRDLRVFDETINTMKVAWQAAPGNVVQYHVTYKLAEGGDINMELVQGDITQALLKNLQPATEYELFVTAHYSTGQGDPLMGAGTTLEELGSPRDLETRDVTDTSFAVSWTAAPGNVRQYRIKWKSMFSEETGEKTVAGDTTTADLDGLTPETRYQVSVFARYGHGEGQPLVGEETTDISANGRVIVVSDETEKSMKVTWQPAPGNVLNYRVTYKPQSGGRQLAAKVPGGNTSTVLRRLTSLTTYDITVVPVYRFGEGKIRQGEGTTLTPYKGPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVSFHPAEDDIDLRELLVSPHDNTVVLEELRAGTKYTVSVFGMFDGGESMPLAGEENTTYSDDPDSSPVDVPKMSCKTKAQADIVLLVDGSWSIGRLNFKTIRSFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHQTRESLLEAVAKLPYKGGNTMTGLALYYILQNNFQESVGMRPGARKIGVLITDGKSQDEIIASSQSLRDAGIELYAIGVKNADENELRAIASDPDEIHMYNVADFSFLLDIVDTLSDNLCNSVTGSDNTPGSPTNLVTSEVTHQSFRATWTGPEGPVEKYRVEYMTVSGAPQEVLVDGTVTTVVLRGLDPLTKYLVNVYSVIGEESSEPLKGTETTLPLSAVREMSVYDEQMTTMRARWKAVQGATGYLLTYRATNATQPTVEQEMRVAAGTTDVQLVKLTPNTAYSLSLYALYGLSASEALLRQGVTLPMPPAGELNVRDVTHSTMVLYWDAAPGAVRKYIITYTPEDGETKELEVGGRITTKLLENLISQTEYSLTVTPVYDEGRGQAMLGSGITDVVPAPKNLRFSEITQTSFRATWEHGAPDVAMYRIAWRRKGETINEGEEILSEEETTFVLENLDPDTPYDVSVTAIYPDESESEDLLGTERTLSKSDGRPIPNAPPTNLVVFNETTTTLNARWTPPLGRVQNYRITYVPTSGGQSQTTMVGAKKNTVLLPKLTPDTEYSIGVVAVYANGVSRELNGLGKTKPLGGVRNLRVTNPTTSTLDVNWEPAEGNVRQYRVFYVPAAGGEQEMIQVSGSTYKAVLKNLQSDTVYTVTVTPVYSTGEGKPMSENGKTLERSPARNIQVFNPTTNTLNVRWEAATGPVQQYRVVYAPLTGVRPTESILVPGTTTTALLQQLVPDTAYNVGVVALYSDGEGTTVSDQGKTLPRGGPRNMRVYDPTTSTLSVNWEHADGPVLQYRISYAPTTGDPIEEFTTVQGNRNNVVLQNLDPDTPYNIRVTAMYAEGAGGELEGDGRTVGMLGPRNLRVSDEWYTRFRVSWDPAPSAVTGYKLIYQPAGSYDSSEVFVGDVTTYQLQNLKPGTTYDLTVLAQYRTGISGPLNGQGTTLYLNVTGLTSYNVGYDSFCIQWVPHRAATSYRLKVNPFESFKGGAQEITVQGPESKYCFDNLTPDTLYNATVYAQTLNLEGPGVSIKEKTLVKPTEVPTEPPTPPPPSIVPDALDMCKGAKADLVFLIDGSWSIGDDSFAKVLQFVTSVTAAFDVVSPTGMQVSFVQYSDDAKTEFKLNTYHNKGIVLSALKSIRYRGGNTKTGIALKHVYEKVLTSDNGMRRNVPKVLVVVTDGRSQDEVKKSAEKLQHSGYSVFVVGVADVSMRELAVIGSKPTERHVFVVDDYDAFSKIQDNLITFICETATSTCPLIYINGYTTPGFRMLEAFNITDRTFAGMNGVSMEPGSFNSFIAYRLHKDAFLNQPTKEIHSDGLPPSYTIILLLRLLPDTPSEPFDIWQISDKNNNPEVGVTMNPSSKTLTFYNKDTRGEIQRAVFNEEQVKRVFHGSFHKLHITVSPEKVKLNVDCQEVAEKPIKEANNITLEGYEVLGKMVKSGGGKRQSATFQLQMFDIVCSLSWISRDKCCDLPATRIESKCPALPHSCTCTQDSVGPQGPPGPSGSPGSKGPRGDRGDPGKPGAMGPRGEPGLQGAMGPPGPQGPNGLSIPGEPGRQGPKGDRGDSGLPGLQGPPGPRGPMGPVGQSGARGPPGKEGSSGPRGPPGPMGSPGNPGVPGMSGKPGKSGEPGSPGPVGLKGEKGERGDFASQNMMRSIARQVCEQLVSSQMSRIDGMLNQIPSGYRNGNPGPAGPPGPPGNQGPRGEPGQPGKSGFPGSPGLPGNQGERGLPGERGERGPPGADIRGQRGPPGPAGPPGESRTGPPGSPGSAGSRGPPGRQGNPGVRGPPGPPGYCDSSQCVGIPYNGQGYTAPRYQPEPDVASIPVEPTEEFETVQSSGYTRNQRGKRSLPLDNSGAS

Target ORF information:

RefSeq Version	XM_011490502.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490502.3

ORF Insert Sequence:

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TGTCCAAGTC AGATGGTAGA CCGATACCAA ATGCACCTCC CACCAATTTG 
GTTGTTTTCA ATGAGACCAC CACCACCCTG AATGCTAGGT GGACTCCACC TCTGGGTCGT 
GTCCAGAACT ACAGGATCAC TTATGTCCCC ACATCTGGAG GCCAGAGTCA GACTACCATG 
GTTGGAGCCA AGAAAAACAC AGTCCTGCTT CCAAAGCTGA CCCCTGACAC AGAGTACTCC 
ATCGGTGTTG TGGCGGTGTA CGCCAATGGA GTCAGCAGGG AGCTGAATGG CCTTGGAAAA 
ACCAAACCTT TGGGTGGTGT TAGAAACCTG CGTGTGACAA ACCCCACCAC GAGCACGTTG 
GATGTGAACT GGGAACCTGC TGAGGGTAAT GTGCGCCAGT ATAGGGTCTT CTATGTTCCT 
GCAGCTGGAG GAGAGCAGGA GATGATTCAG GTCTCCGGAA GCACCTACAA AGCAGTTCTG 
AAGAACCTGC AGTCGGACAC TGTCTACACT GTCACAGTGA CTCCTGTTTA TTCTACTGGA 
GAGGGGAAGC CCATGTCTGA AAACGGCAAA ACATTGGAGC GCAGTCCTGC TAGGAATATC 
CAGGTTTTCA ACCCCACAAC TAACACTTTG AATGTCCGCT GGGAAGCAGC CACCGGGCCG 
GTCCAGCAGT ACAGGGTGGT CTATGCTCCT TTGACTGGTG TGAGGCCAAC GGAGTCGATT 
TTGGTCCCCG GCACCACAAC CACAGCACTG CTGCAGCAGC TGGTCCCAGA CACAGCCTAC 
AATGTGGGAG TTGTTGCCTT GTACAGCGAT GGGGAAGGAA CCACTGTCAG CGATCAGGGA 
AAGACTTTGC CCCGGGGTGG TCCCAGAAAC ATGCGTGTGT ACGATCCCAC CACCAGTACG 
CTGTCTGTTA ACTGGGAACA CGCTGACGGT CCTGTTCTTC AGTACCGCAT CAGCTATGCC 
CCAACAACAG GAGACCCTAT CGAGGAATTT ACCACAGTTC AAGGGAACAG GAACAATGTG 
GTTCTTCAGA ATCTGGATCC AGACACTCCC TACAATATCA GAGTGACTGC TATGTATGCT 
GAAGGAGCGG GAGGAGAACT GGAGGGAGAT GGACGTACAG TTGGAATGCT CGGCCCCAGA 
AACCTGCGTG TATCTGATGA ATGGTACACT CGCTTCAGGG TGTCCTGGGA CCCCGCCCCC 
TCCGCAGTCA CTGGATACAA ACTCATCTAC CAGCCTGCGG GGTCTTATGA CTCATCTGAG 
GTGTTTGTTG GAGATGTGAC CACCTATCAG CTGCAAAACT TGAAGCCTGG AACTACCTAT 
GACTTGACGG TCCTAGCACA GTACAGAACG GGCATCAGTG GTCCTCTAAA TGGTCAAGGC 
ACCACATTGT ACCTCAATGT GACAGGCCTA ACCTCCTACA ATGTTGGCTA TGACTCCTTT 
TGCATCCAGT GGGTCCCTCA CCGAGCAGCC ACGTCGTACA GACTTAAAGT CAACCCTTTT 
GAGTCCTTCA AGGGTGGAGC TCAAGAAATC ACAGTTCAGG GCCCAGAGAG TAAATACTGC 
TTTGACAACC TGACCCCTGA CACTCTTTAC AACGCTACGG TTTACGCTCA GACCCTCAAC 
CTTGAAGGAC CTGGAGTCAG TATTAAGGAG AAAACATTGG TCAAACCCAC CGAGGTGCCA 
ACCGAGCCTC CCACCCCTCC CCCTCCATCA ATAGTGCCTG ATGCTCTTGA CATGTGTAAA 
GGTGCGAAGG CTGACTTGGT CTTCCTCATC GACGGCTCAT GGAGTATTGG AGATGACAGC 
TTTGCCAAAG TCCTCCAGTT TGTCACTAGT GTGACAGCAG CTTTTGATGT TGTTAGCCCA 
ACTGGAATGC AGGTTTCATT TGTGCAGTAT AGCGATGATG CTAAGACTGA GTTCAAACTG 
AACACCTACC ACAACAAGGG CATTGTCCTA TCTGCACTGA AGTCAATCCG CTACAGGGGA 
GGCAACACTA AGACTGGCAT TGCTCTGAAG CACGTCTATG AAAAGGTACT AACGTCCGAC 
AATGGCATGA GGAGGAATGT CCCGAAGGTG CTGGTTGTTG TCACAGATGG TCGTTCTCAA 
GATGAAGTCA AGAAGAGCGC AGAAAAACTT CAGCATTCTG GCTATAGTGT GTTTGTGGTA 
GGAGTGGCTG ATGTGAGCAT GAGAGAACTG GCAGTTATCG GCAGCAAGCC CACCGAAAGA 
CACGTGTTTG TCGTTGACGA TTACGACGCT TTTTCCAAAA TCCAGGACAA CTTGATCACA 
TTCATCTGTG AAACGGCTAC CTCCACTTGC CCTCTCATCT ACATCAATGG ATACACCACA 
CCTGGATTTA GGATGCTGGA AGCTTTCAAC ATCACAGACC GAACATTTGC TGGCATGAAT 
GGTGTCTCCA TGGAGCCAGG TTCTTTCAAC AGCTTCATTG CCTACAGGCT GCACAAAGAT 
GCCTTCCTAA ACCAGCCCAC AAAGGAAATC CACTCCGACG GCCTTCCTCC ATCCTACACT 
ATAATCCTGC TCCTCCGCCT GCTGCCTGAT ACACCCTCTG AACCTTTTGA TATCTGGCAG 
ATCTCTGACA AAAACAACAA CCCTGAAGTT GGAGTCACTA TGAATCCTTC CAGCAAAACA 
CTCACGTTCT ACAACAAGGA CACCCGGGGA GAGATCCAGA GAGCCGTGTT CAATGAGGAG 
CAAGTGAAAC GGGTTTTCCA CGGAAGCTTC CACAAGCTCC ACATTACCGT CTCCCCAGAG 
AAGGTCAAGC TCAACGTGGA CTGCCAAGAG GTGGCAGAGA AGCCAATCAA GGAGGCCAAC 
AACATCACGC TGGAGGGCTA TGAAGTGCTT GGCAAGATGG TCAAATCAGG AGGTGGAAAG 
AGGCAGTCCG CTACATTCCA GCTCCAGATG TTTGACATTG TCTGCAGCTT GAGTTGGATC 
AGCCGTGACA AATGCTGTGA CCTGCCTGCT ACGAGAATTG AGTCCAAATG TCCTGCTCTT 
CCCCACTCCT GCACATGCAC CCAAGACAGT GTTGGGCCCC AGGGGCCTCC TGGGCCTTCG 
GGCAGCCCAG GGTCTAAAGG ACCCAGAGGA GACAGGGGAG ACCCAGGCAA ACCTGGTGCA 
ATGGGTCCAC GAGGTGAGCC AGGACTCCAA GGTGCAATGG GCCCACCAGG CCCACAAGGC 
CCTAATGGAC TGTCTATCCC CGGAGAACCG GGTCGCCAAG GACCAAAGGG AGATCGTGGA 
GACTCAGGCC TGCCTGGGCT GCAAGGTCCT CCTGGGCCGC GTGGTCCTAT GGGTCCTGTC 
GGACAAAGTG GAGCACGGGG GCCACCAGGA AAGGAAGGAT CATCTGGACC CAGAGGCCCA 
CCAGGGCCCA TGGGAAGTCC AGGAAATCCA GGTGTACCTG GTATGTCTGG AAAACCAGGA 
AAGTCAGGAG AACCAGGAAG TCCAGGACCT GTTGGCCTTA AAGGAGAAAA GGGAGAGCGG 
GGAGACTTTG CATCCCAAAA TATGATGCGC TCCATTGCCA GACAAGTTTG TGAACAGCTC 
GTGAGCAGTC AGATGAGCAG AATTGACGGG ATGCTCAATC AAATTCCTTC TGGATATCGT 
AATGGCAATC CAGGCCCGGC TGGTCCTCCT GGTCCCCCTG GCAACCAAGG ACCTCGTGGA 
GAGCCCGGAC AACCTGGAAA ATCTGGTTTC CCTGGAAGCC CAGGATTACC TGGAAATCAA 
GGAGAAAGAG GGTTACCAGG AGAGAGGGGG GAGAGAGGAC CTCCAGGAGC AGATATCAGA 
GGACAAAGAG GTCCACCTGG ACCAGCAGGT CCACCAGGGG AGTCTAGAAC AGGTCCCCCA 
GGTTCTCCTG GCTCTGCTGG ATCCCGTGGC CCCCCAGGTC GTCAAGGTAA TCCAGGTGTG 
AGAGGACCAC CAGGACCCCC TGGATACTGT GACTCCTCTC AGTGTGTTGG TATTCCTTAC 
AATGGACAAG GATACACAGC TCCACGCTAC CAGCCTGAAC CTGATGTAGC GTCTATACCT 
GTGGAGCCAA CTGAAGAGTT TGAGACAGTA CAATCATCAG GTTATACAAG GAACCAACGG 
GGAAAGAGAT CGCTACCACT AGACAATTCA GGAGCATCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col12a1 ( XM_011490502.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488804.1 Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X1, mRNA.

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CloneID	OJa24261
Clone ID Related Accession (Same CDS sequence)	XM_011490502.3 , XM_011490502.2
Accession Version	XM_011490502.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	collagen alpha-1(XII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011490502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TGTCCAAGTC AGATGGTAGA CCGATACCAA ATGCACCTCC CACCAATTTG 
GTTGTTTTCA ATGAGACCAC CACCACCCTG AATGCTAGGT GGACTCCACC TCTGGGTCGT 
GTCCAGAACT ACAGGATCAC TTATGTCCCC ACATCTGGAG GCCAGAGTCA GACTACCATG 
GTTGGAGCCA AGAAAAACAC AGTCCTGCTT CCAAAGCTGA CCCCTGACAC AGAGTACTCC 
ATCGGTGTTG TGGCGGTGTA CGCCAATGGA GTCAGCAGGG AGCTGAATGG CCTTGGAAAA 
ACCAAACCTT TGGGTGGTGT TAGAAACCTG CGTGTGACAA ACCCCACCAC GAGCACGTTG 
GATGTGAACT GGGAACCTGC TGAGGGTAAT GTGCGCCAGT ATAGGGTCTT CTATGTTCCT 
GCAGCTGGAG GAGAGCAGGA GATGATTCAG GTCTCCGGAA GCACCTACAA AGCAGTTCTG 
AAGAACCTGC AGTCGGACAC TGTCTACACT GTCACAGTGA CTCCTGTTTA TTCTACTGGA 
GAGGGGAAGC CCATGTCTGA AAACGGCAAA ACATTGGAGC GCAGTCCTGC TAGGAATATC 
CAGGTTTTCA ACCCCACAAC TAACACTTTG AATGTCCGCT GGGAAGCAGC CACCGGGCCG 
GTCCAGCAGT ACAGGGTGGT CTATGCTCCT TTGACTGGTG TGAGGCCAAC GGAGTCGATT 
TTGGTCCCCG GCACCACAAC CACAGCACTG CTGCAGCAGC TGGTCCCAGA CACAGCCTAC 
AATGTGGGAG TTGTTGCCTT GTACAGCGAT GGGGAAGGAA CCACTGTCAG CGATCAGGGA 
AAGACTTTGC CCCGGGGTGG TCCCAGAAAC ATGCGTGTGT ACGATCCCAC CACCAGTACG 
CTGTCTGTTA ACTGGGAACA CGCTGACGGT CCTGTTCTTC AGTACCGCAT CAGCTATGCC 
CCAACAACAG GAGACCCTAT CGAGGAATTT ACCACAGTTC AAGGGAACAG GAACAATGTG 
GTTCTTCAGA ATCTGGATCC AGACACTCCC TACAATATCA GAGTGACTGC TATGTATGCT 
GAAGGAGCGG GAGGAGAACT GGAGGGAGAT GGACGTACAG TTGGAATGCT CGGCCCCAGA 
AACCTGCGTG TATCTGATGA ATGGTACACT CGCTTCAGGG TGTCCTGGGA CCCCGCCCCC 
TCCGCAGTCA CTGGATACAA ACTCATCTAC CAGCCTGCGG GGTCTTATGA CTCATCTGAG 
GTGTTTGTTG GAGATGTGAC CACCTATCAG CTGCAAAACT TGAAGCCTGG AACTACCTAT 
GACTTGACGG TCCTAGCACA GTACAGAACG GGCATCAGTG GTCCTCTAAA TGGTCAAGGC 
ACCACATTGT ACCTCAATGT GACAGGCCTA ACCTCCTACA ATGTTGGCTA TGACTCCTTT 
TGCATCCAGT GGGTCCCTCA CCGAGCAGCC ACGTCGTACA GACTTAAAGT CAACCCTTTT 
GAGTCCTTCA AGGGTGGAGC TCAAGAAATC ACAGTTCAGG GCCCAGAGAG TAAATACTGC 
TTTGACAACC TGACCCCTGA CACTCTTTAC AACGCTACGG TTTACGCTCA GACCCTCAAC 
CTTGAAGGAC CTGGAGTCAG TATTAAGGAG AAAACATTGG TCAAACCCAC CGAGGTGCCA 
ACCGAGCCTC CCACCCCTCC CCCTCCATCA ATAGTGCCTG ATGCTCTTGA CATGTGTAAA 
GGTGCGAAGG CTGACTTGGT CTTCCTCATC GACGGCTCAT GGAGTATTGG AGATGACAGC 
TTTGCCAAAG TCCTCCAGTT TGTCACTAGT GTGACAGCAG CTTTTGATGT TGTTAGCCCA 
ACTGGAATGC AGGTTTCATT TGTGCAGTAT AGCGATGATG CTAAGACTGA GTTCAAACTG 
AACACCTACC ACAACAAGGG CATTGTCCTA TCTGCACTGA AGTCAATCCG CTACAGGGGA 
GGCAACACTA AGACTGGCAT TGCTCTGAAG CACGTCTATG AAAAGGTACT AACGTCCGAC 
AATGGCATGA GGAGGAATGT CCCGAAGGTG CTGGTTGTTG TCACAGATGG TCGTTCTCAA 
GATGAAGTCA AGAAGAGCGC AGAAAAACTT CAGCATTCTG GCTATAGTGT GTTTGTGGTA 
GGAGTGGCTG ATGTGAGCAT GAGAGAACTG GCAGTTATCG GCAGCAAGCC CACCGAAAGA 
CACGTGTTTG TCGTTGACGA TTACGACGCT TTTTCCAAAA TCCAGGACAA CTTGATCACA 
TTCATCTGTG AAACGGCTAC CTCCACTTGC CCTCTCATCT ACATCAATGG ATACACCACA 
CCTGGATTTA GGATGCTGGA AGCTTTCAAC ATCACAGACC GAACATTTGC TGGCATGAAT 
GGTGTCTCCA TGGAGCCAGG TTCTTTCAAC AGCTTCATTG CCTACAGGCT GCACAAAGAT 
GCCTTCCTAA ACCAGCCCAC AAAGGAAATC CACTCCGACG GCCTTCCTCC ATCCTACACT 
ATAATCCTGC TCCTCCGCCT GCTGCCTGAT ACACCCTCTG AACCTTTTGA TATCTGGCAG 
ATCTCTGACA AAAACAACAA CCCTGAAGTT GGAGTCACTA TGAATCCTTC CAGCAAAACA 
CTCACGTTCT ACAACAAGGA CACCCGGGGA GAGATCCAGA GAGCCGTGTT CAATGAGGAG 
CAAGTGAAAC GGGTTTTCCA CGGAAGCTTC CACAAGCTCC ACATTACCGT CTCCCCAGAG 
AAGGTCAAGC TCAACGTGGA CTGCCAAGAG GTGGCAGAGA AGCCAATCAA GGAGGCCAAC 
AACATCACGC TGGAGGGCTA TGAAGTGCTT GGCAAGATGG TCAAATCAGG AGGTGGAAAG 
AGGCAGTCCG CTACATTCCA GCTCCAGATG TTTGACATTG TCTGCAGCTT GAGTTGGATC 
AGCCGTGACA AATGCTGTGA CCTGCCTGCT ACGAGAATTG AGTCCAAATG TCCTGCTCTT 
CCCCACTCCT GCACATGCAC CCAAGACAGT GTTGGGCCCC AGGGGCCTCC TGGGCCTTCG 
GGCAGCCCAG GGTCTAAAGG ACCCAGAGGA GACAGGGGAG ACCCAGGCAA ACCTGGTGCA 
ATGGGTCCAC GAGGTGAGCC AGGACTCCAA GGTGCAATGG GCCCACCAGG CCCACAAGGC 
CCTAATGGAC TGTCTATCCC CGGAGAACCG GGTCGCCAAG GACCAAAGGG AGATCGTGGA 
GACTCAGGCC TGCCTGGGCT GCAAGGTCCT CCTGGGCCGC GTGGTCCTAT GGGTCCTGTC 
GGACAAAGTG GAGCACGGGG GCCACCAGGA AAGGAAGGAT CATCTGGACC CAGAGGCCCA 
CCAGGGCCCA TGGGAAGTCC AGGAAATCCA GGTGTACCTG GTATGTCTGG AAAACCAGGA 
AAGTCAGGAG AACCAGGAAG TCCAGGACCT GTTGGCCTTA AAGGAGAAAA GGGAGAGCGG 
GGAGACTTTG CATCCCAAAA TATGATGCGC TCCATTGCCA GACAAGTTTG TGAACAGCTC 
GTGAGCAGTC AGATGAGCAG AATTGACGGG ATGCTCAATC AAATTCCTTC TGGATATCGT 
AATGGCAATC CAGGCCCGGC TGGTCCTCCT GGTCCCCCTG GCAACCAAGG ACCTCGTGGA 
GAGCCCGGAC AACCTGGAAA ATCTGGTTTC CCTGGAAGCC CAGGATTACC TGGAAATCAA 
GGAGAAAGAG GGTTACCAGG AGAGAGGGGG GAGAGAGGAC CTCCAGGAGC AGATATCAGA 
GGACAAAGAG GTCCACCTGG ACCAGCAGGT CCACCAGGGG AGTCTAGAAC AGGTCCCCCA 
GGTTCTCCTG GCTCTGCTGG ATCCCGTGGC CCCCCAGGTC GTCAAGGTAA TCCAGGTGTG 
AGAGGACCAC CAGGACCCCC TGGATACTGT GACTCCTCTC AGTGTGTTGG TATTCCTTAC 
AATGGACAAG GATACACAGC TCCACGCTAC CAGCCTGAAC CTGATGTAGC GTCTATACCT 
GTGGAGCCAA CTGAAGAGTT TGAGACAGTA CAATCATCAG GTTATACAAG GAACCAACGG 
GGAAAGAGAT CGCTACCACT AGACAATTCA GGAGCATCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488804.1
CDS	231..9572
Translation	MKIRLSLATAAFLVGFLASIDAQVEPPSDLKFKILNENTVEMSWLKPSSRIEGFRIQVASDADEPSRDFTLDAYSTSTSITNLTPDVDYSVSISSYYGSEESISIFGQLTIQSSNTTRPRPTSDALKCSVSAIADLVFLVDGSWSVGRENFKHIRSFIGSLAGAFDIGEDKTRVAVVQYSTDTRTEFPLNRYTRRGDLLQAINSLPYKGGNTMTGDAIDYLIKNIFTEAAGSRKAFPKVAMIITDGKSQDPVEEHARRLRNIGVEIFVLGIKGADEDELREIASTPHAKHVYNVPNFDLIQEVQKRIITEVCSGVDDQLTSLVSGEEMVEAPSNLQVTEIASKSMRLTWDASLGDITGYKLTLIPLVPGMKRQELYMGATQTSINVRDLSPETEYDISLFALKGLTPSEPLTDLAKTQPVKVLTECSMDVDVQADVVLLVDGSYSIGLQNFAKVREFLEVLVKSFDIGPDKIQISLVQYSRDPHTEFYLNTHHDINAVVKAVRNFPYRGGSTNTGKAMTYVREKIFVPTRGARQNVPRVMVLITDGKSSDSFRVAATKLRNIDVEIFAVGVKDAVRSELEAIANEPADTHVYEVEDFDAFQRISKELTQSICLRIEQELLKIKKKNLLPPSNLQFSEITSRSFRATWVAPAASVMSYLVRYRKAEDITGDYISLAVPGQTTTTVLLYLTPITTYEVNVYAEYDKGDSFALTGQETTLEEQGTVENLRVTEETTNSFRVSWQAAPGSVIRYRLSYVPLSGMGEILEAQTLGPETTIVLQELFPITTYRVSVSPEYAAGVGDELQVDGTTKEVIGSPRDLRVFDETINTMKVAWQAAPGNVVQYHVTYKLAEGGDINMELVQGDITQALLKNLQPATEYELFVTAHYSTGQGDPLMGAGTTLEELGSPRDLETRDVTDTSFAVSWTAAPGNVRQYRIKWKSMFSEETGEKTVAGDTTTADLDGLTPETRYQVSVFARYGHGEGQPLVGEETTDISANGRVIVVSDETEKSMKVTWQPAPGNVLNYRVTYKPQSGGRQLAAKVPGGNTSTVLRRLTSLTTYDITVVPVYRFGEGKIRQGEGTTLTPYKGPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVSFHPAEDDIDLRELLVSPHDNTVVLEELRAGTKYTVSVFGMFDGGESMPLAGEENTTYSDDPDSSPVDVPKMSCKTKAQADIVLLVDGSWSIGRLNFKTIRSFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHQTRESLLEAVAKLPYKGGNTMTGLALYYILQNNFQESVGMRPGARKIGVLITDGKSQDEIIASSQSLRDAGIELYAIGVKNADENELRAIASDPDEIHMYNVADFSFLLDIVDTLSDNLCNSVTGSDNTPGSPTNLVTSEVTHQSFRATWTGPEGPVEKYRVEYMTVSGAPQEVLVDGTVTTVVLRGLDPLTKYLVNVYSVIGEESSEPLKGTETTLPLSAVREMSVYDEQMTTMRARWKAVQGATGYLLTYRATNATQPTVEQEMRVAAGTTDVQLVKLTPNTAYSLSLYALYGLSASEALLRQGVTLPMPPAGELNVRDVTHSTMVLYWDAAPGAVRKYIITYTPEDGETKELEVGGRITTKLLENLISQTEYSLTVTPVYDEGRGQAMLGSGITDVVPAPKNLRFSEITQTSFRATWEHGAPDVAMYRIAWRRKGETINEGEEILSEEETTFVLENLDPDTPYDVSVTAIYPDESESEDLLGTERTLSKSDGRPIPNAPPTNLVVFNETTTTLNARWTPPLGRVQNYRITYVPTSGGQSQTTMVGAKKNTVLLPKLTPDTEYSIGVVAVYANGVSRELNGLGKTKPLGGVRNLRVTNPTTSTLDVNWEPAEGNVRQYRVFYVPAAGGEQEMIQVSGSTYKAVLKNLQSDTVYTVTVTPVYSTGEGKPMSENGKTLERSPARNIQVFNPTTNTLNVRWEAATGPVQQYRVVYAPLTGVRPTESILVPGTTTTALLQQLVPDTAYNVGVVALYSDGEGTTVSDQGKTLPRGGPRNMRVYDPTTSTLSVNWEHADGPVLQYRISYAPTTGDPIEEFTTVQGNRNNVVLQNLDPDTPYNIRVTAMYAEGAGGELEGDGRTVGMLGPRNLRVSDEWYTRFRVSWDPAPSAVTGYKLIYQPAGSYDSSEVFVGDVTTYQLQNLKPGTTYDLTVLAQYRTGISGPLNGQGTTLYLNVTGLTSYNVGYDSFCIQWVPHRAATSYRLKVNPFESFKGGAQEITVQGPESKYCFDNLTPDTLYNATVYAQTLNLEGPGVSIKEKTLVKPTEVPTEPPTPPPPSIVPDALDMCKGAKADLVFLIDGSWSIGDDSFAKVLQFVTSVTAAFDVVSPTGMQVSFVQYSDDAKTEFKLNTYHNKGIVLSALKSIRYRGGNTKTGIALKHVYEKVLTSDNGMRRNVPKVLVVVTDGRSQDEVKKSAEKLQHSGYSVFVVGVADVSMRELAVIGSKPTERHVFVVDDYDAFSKIQDNLITFICETATSTCPLIYINGYTTPGFRMLEAFNITDRTFAGMNGVSMEPGSFNSFIAYRLHKDAFLNQPTKEIHSDGLPPSYTIILLLRLLPDTPSEPFDIWQISDKNNNPEVGVTMNPSSKTLTFYNKDTRGEIQRAVFNEEQVKRVFHGSFHKLHITVSPEKVKLNVDCQEVAEKPIKEANNITLEGYEVLGKMVKSGGGKRQSATFQLQMFDIVCSLSWISRDKCCDLPATRIESKCPALPHSCTCTQDSVGPQGPPGPSGSPGSKGPRGDRGDPGKPGAMGPRGEPGLQGAMGPPGPQGPNGLSIPGEPGRQGPKGDRGDSGLPGLQGPPGPRGPMGPVGQSGARGPPGKEGSSGPRGPPGPMGSPGNPGVPGMSGKPGKSGEPGSPGPVGLKGEKGERGDFASQNMMRSIARQVCEQLVSSQMSRIDGMLNQIPSGYRNGNPGPAGPPGPPGNQGPRGEPGQPGKSGFPGSPGLPGNQGERGLPGERGERGPPGADIRGQRGPPGPAGPPGESRTGPPGSPGSAGSRGPPGRQGNPGVRGPPGPPGYCDSSQCVGIPYNGQGYTAPRYQPEPDVASIPVEPTEEFETVQSSGYTRNQRGKRSLPLDNSGAS

Target ORF information:

RefSeq Version	XM_011490502.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490502.2

ORF Insert Sequence:

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TGTCCAAGTC AGATGGTAGA CCGATACCAA ATGCACCTCC CACCAATTTG 
GTTGTTTTCA ATGAGACCAC CACCACCCTG AATGCTAGGT GGACTCCACC TCTGGGTCGT 
GTCCAGAACT ACAGGATCAC TTATGTCCCC ACATCTGGAG GCCAGAGTCA GACTACCATG 
GTTGGAGCCA AGAAAAACAC AGTCCTGCTT CCAAAGCTGA CCCCTGACAC AGAGTACTCC 
ATCGGTGTTG TGGCGGTGTA CGCCAATGGA GTCAGCAGGG AGCTGAATGG CCTTGGAAAA 
ACCAAACCTT TGGGTGGTGT TAGAAACCTG CGTGTGACAA ACCCCACCAC GAGCACGTTG 
GATGTGAACT GGGAACCTGC TGAGGGTAAT GTGCGCCAGT ATAGGGTCTT CTATGTTCCT 
GCAGCTGGAG GAGAGCAGGA GATGATTCAG GTCTCCGGAA GCACCTACAA AGCAGTTCTG 
AAGAACCTGC AGTCGGACAC TGTCTACACT GTCACAGTGA CTCCTGTTTA TTCTACTGGA 
GAGGGGAAGC CCATGTCTGA AAACGGCAAA ACATTGGAGC GCAGTCCTGC TAGGAATATC 
CAGGTTTTCA ACCCCACAAC TAACACTTTG AATGTCCGCT GGGAAGCAGC CACCGGGCCG 
GTCCAGCAGT ACAGGGTGGT CTATGCTCCT TTGACTGGTG TGAGGCCAAC GGAGTCGATT 
TTGGTCCCCG GCACCACAAC CACAGCACTG CTGCAGCAGC TGGTCCCAGA CACAGCCTAC 
AATGTGGGAG TTGTTGCCTT GTACAGCGAT GGGGAAGGAA CCACTGTCAG CGATCAGGGA 
AAGACTTTGC CCCGGGGTGG TCCCAGAAAC ATGCGTGTGT ACGATCCCAC CACCAGTACG 
CTGTCTGTTA ACTGGGAACA CGCTGACGGT CCTGTTCTTC AGTACCGCAT CAGCTATGCC 
CCAACAACAG GAGACCCTAT CGAGGAATTT ACCACAGTTC AAGGGAACAG GAACAATGTG 
GTTCTTCAGA ATCTGGATCC AGACACTCCC TACAATATCA GAGTGACTGC TATGTATGCT 
GAAGGAGCGG GAGGAGAACT GGAGGGAGAT GGACGTACAG TTGGAATGCT CGGCCCCAGA 
AACCTGCGTG TATCTGATGA ATGGTACACT CGCTTCAGGG TGTCCTGGGA CCCCGCCCCC 
TCCGCAGTCA CTGGATACAA ACTCATCTAC CAGCCTGCGG GGTCTTATGA CTCATCTGAG 
GTGTTTGTTG GAGATGTGAC CACCTATCAG CTGCAAAACT TGAAGCCTGG AACTACCTAT 
GACTTGACGG TCCTAGCACA GTACAGAACG GGCATCAGTG GTCCTCTAAA TGGTCAAGGC 
ACCACATTGT ACCTCAATGT GACAGGCCTA ACCTCCTACA ATGTTGGCTA TGACTCCTTT 
TGCATCCAGT GGGTCCCTCA CCGAGCAGCC ACGTCGTACA GACTTAAAGT CAACCCTTTT 
GAGTCCTTCA AGGGTGGAGC TCAAGAAATC ACAGTTCAGG GCCCAGAGAG TAAATACTGC 
TTTGACAACC TGACCCCTGA CACTCTTTAC AACGCTACGG TTTACGCTCA GACCCTCAAC 
CTTGAAGGAC CTGGAGTCAG TATTAAGGAG AAAACATTGG TCAAACCCAC CGAGGTGCCA 
ACCGAGCCTC CCACCCCTCC CCCTCCATCA ATAGTGCCTG ATGCTCTTGA CATGTGTAAA 
GGTGCGAAGG CTGACTTGGT CTTCCTCATC GACGGCTCAT GGAGTATTGG AGATGACAGC 
TTTGCCAAAG TCCTCCAGTT TGTCACTAGT GTGACAGCAG CTTTTGATGT TGTTAGCCCA 
ACTGGAATGC AGGTTTCATT TGTGCAGTAT AGCGATGATG CTAAGACTGA GTTCAAACTG 
AACACCTACC ACAACAAGGG CATTGTCCTA TCTGCACTGA AGTCAATCCG CTACAGGGGA 
GGCAACACTA AGACTGGCAT TGCTCTGAAG CACGTCTATG AAAAGGTACT AACGTCCGAC 
AATGGCATGA GGAGGAATGT CCCGAAGGTG CTGGTTGTTG TCACAGATGG TCGTTCTCAA 
GATGAAGTCA AGAAGAGCGC AGAAAAACTT CAGCATTCTG GCTATAGTGT GTTTGTGGTA 
GGAGTGGCTG ATGTGAGCAT GAGAGAACTG GCAGTTATCG GCAGCAAGCC CACCGAAAGA 
CACGTGTTTG TCGTTGACGA TTACGACGCT TTTTCCAAAA TCCAGGACAA CTTGATCACA 
TTCATCTGTG AAACGGCTAC CTCCACTTGC CCTCTCATCT ACATCAATGG ATACACCACA 
CCTGGATTTA GGATGCTGGA AGCTTTCAAC ATCACAGACC GAACATTTGC TGGCATGAAT 
GGTGTCTCCA TGGAGCCAGG TTCTTTCAAC AGCTTCATTG CCTACAGGCT GCACAAAGAT 
GCCTTCCTAA ACCAGCCCAC AAAGGAAATC CACTCCGACG GCCTTCCTCC ATCCTACACT 
ATAATCCTGC TCCTCCGCCT GCTGCCTGAT ACACCCTCTG AACCTTTTGA TATCTGGCAG 
ATCTCTGACA AAAACAACAA CCCTGAAGTT GGAGTCACTA TGAATCCTTC CAGCAAAACA 
CTCACGTTCT ACAACAAGGA CACCCGGGGA GAGATCCAGA GAGCCGTGTT CAATGAGGAG 
CAAGTGAAAC GGGTTTTCCA CGGAAGCTTC CACAAGCTCC ACATTACCGT CTCCCCAGAG 
AAGGTCAAGC TCAACGTGGA CTGCCAAGAG GTGGCAGAGA AGCCAATCAA GGAGGCCAAC 
AACATCACGC TGGAGGGCTA TGAAGTGCTT GGCAAGATGG TCAAATCAGG AGGTGGAAAG 
AGGCAGTCCG CTACATTCCA GCTCCAGATG TTTGACATTG TCTGCAGCTT GAGTTGGATC 
AGCCGTGACA AATGCTGTGA CCTGCCTGCT ACGAGAATTG AGTCCAAATG TCCTGCTCTT 
CCCCACTCCT GCACATGCAC CCAAGACAGT GTTGGGCCCC AGGGGCCTCC TGGGCCTTCG 
GGCAGCCCAG GGTCTAAAGG ACCCAGAGGA GACAGGGGAG ACCCAGGCAA ACCTGGTGCA 
ATGGGTCCAC GAGGTGAGCC AGGACTCCAA GGTGCAATGG GCCCACCAGG CCCACAAGGC 
CCTAATGGAC TGTCTATCCC CGGAGAACCG GGTCGCCAAG GACCAAAGGG AGATCGTGGA 
GACTCAGGCC TGCCTGGGCT GCAAGGTCCT CCTGGGCCGC GTGGTCCTAT GGGTCCTGTC 
GGACAAAGTG GAGCACGGGG GCCACCAGGA AAGGAAGGAT CATCTGGACC CAGAGGCCCA 
CCAGGGCCCA TGGGAAGTCC AGGAAATCCA GGTGTACCTG GTATGTCTGG AAAACCAGGA 
AAGTCAGGAG AACCAGGAAG TCCAGGACCT GTTGGCCTTA AAGGAGAAAA GGGAGAGCGG 
GGAGACTTTG CATCCCAAAA TATGATGCGC TCCATTGCCA GACAAGTTTG TGAACAGCTC 
GTGAGCAGTC AGATGAGCAG AATTGACGGG ATGCTCAATC AAATTCCTTC TGGATATCGT 
AATGGCAATC CAGGCCCGGC TGGTCCTCCT GGTCCCCCTG GCAACCAAGG ACCTCGTGGA 
GAGCCCGGAC AACCTGGAAA ATCTGGTTTC CCTGGAAGCC CAGGATTACC TGGAAATCAA 
GGAGAAAGAG GGTTACCAGG AGAGAGGGGG GAGAGAGGAC CTCCAGGAGC AGATATCAGA 
GGACAAAGAG GTCCACCTGG ACCAGCAGGT CCACCAGGGG AGTCTAGAAC AGGTCCCCCA 
GGTTCTCCTG GCTCTGCTGG ATCCCGTGGC CCCCCAGGTC GTCAAGGTAA TCCAGGTGTG 
AGAGGACCAC CAGGACCCCC TGGATACTGT GACTCCTCTC AGTGTGTTGG TATTCCTTAC 
AATGGACAAG GATACACAGC TCCACGCTAC CAGCCTGAAC CTGATGTAGC GTCTATACCT 
GTGGAGCCAA CTGAAGAGTT TGAGACAGTA CAATCATCAG GTTATACAAG GAACCAACGG 
GGAAAGAGAT CGCTACCACT AGACAATTCA GGAGCATCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col12a1 ( XM_011490503.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488805.1 Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X2, mRNA.

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CloneID	OJa24262
Clone ID Related Accession (Same CDS sequence)	XM_011490503.3 , XM_011490503.2
Accession Version	XM_011490503.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	collagen alpha-1(XII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011490503.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TACCAAATGC ACCTCCCACC AATTTGGTTG TTTTCAATGA GACCACCACC 
ACCCTGAATG CTAGGTGGAC TCCACCTCTG GGTCGTGTCC AGAACTACAG GATCACTTAT 
GTCCCCACAT CTGGAGGCCA GAGTCAGACT ACCATGGTTG GAGCCAAGAA AAACACAGTC 
CTGCTTCCAA AGCTGACCCC TGACACAGAG TACTCCATCG GTGTTGTGGC GGTGTACGCC 
AATGGAGTCA GCAGGGAGCT GAATGGCCTT GGAAAAACCA AACCTTTGGG TGGTGTTAGA 
AACCTGCGTG TGACAAACCC CACCACGAGC ACGTTGGATG TGAACTGGGA ACCTGCTGAG 
GGTAATGTGC GCCAGTATAG GGTCTTCTAT GTTCCTGCAG CTGGAGGAGA GCAGGAGATG 
ATTCAGGTCT CCGGAAGCAC CTACAAAGCA GTTCTGAAGA ACCTGCAGTC GGACACTGTC 
TACACTGTCA CAGTGACTCC TGTTTATTCT ACTGGAGAGG GGAAGCCCAT GTCTGAAAAC 
GGCAAAACAT TGGAGCGCAG TCCTGCTAGG AATATCCAGG TTTTCAACCC CACAACTAAC 
ACTTTGAATG TCCGCTGGGA AGCAGCCACC GGGCCGGTCC AGCAGTACAG GGTGGTCTAT 
GCTCCTTTGA CTGGTGTGAG GCCAACGGAG TCGATTTTGG TCCCCGGCAC CACAACCACA 
GCACTGCTGC AGCAGCTGGT CCCAGACACA GCCTACAATG TGGGAGTTGT TGCCTTGTAC 
AGCGATGGGG AAGGAACCAC TGTCAGCGAT CAGGGAAAGA CTTTGCCCCG GGGTGGTCCC 
AGAAACATGC GTGTGTACGA TCCCACCACC AGTACGCTGT CTGTTAACTG GGAACACGCT 
GACGGTCCTG TTCTTCAGTA CCGCATCAGC TATGCCCCAA CAACAGGAGA CCCTATCGAG 
GAATTTACCA CAGTTCAAGG GAACAGGAAC AATGTGGTTC TTCAGAATCT GGATCCAGAC 
ACTCCCTACA ATATCAGAGT GACTGCTATG TATGCTGAAG GAGCGGGAGG AGAACTGGAG 
GGAGATGGAC GTACAGTTGG AATGCTCGGC CCCAGAAACC TGCGTGTATC TGATGAATGG 
TACACTCGCT TCAGGGTGTC CTGGGACCCC GCCCCCTCCG CAGTCACTGG ATACAAACTC 
ATCTACCAGC CTGCGGGGTC TTATGACTCA TCTGAGGTGT TTGTTGGAGA TGTGACCACC 
TATCAGCTGC AAAACTTGAA GCCTGGAACT ACCTATGACT TGACGGTCCT AGCACAGTAC 
AGAACGGGCA TCAGTGGTCC TCTAAATGGT CAAGGCACCA CATTGTACCT CAATGTGACA 
GGCCTAACCT CCTACAATGT TGGCTATGAC TCCTTTTGCA TCCAGTGGGT CCCTCACCGA 
GCAGCCACGT CGTACAGACT TAAAGTCAAC CCTTTTGAGT CCTTCAAGGG TGGAGCTCAA 
GAAATCACAG TTCAGGGCCC AGAGAGTAAA TACTGCTTTG ACAACCTGAC CCCTGACACT 
CTTTACAACG CTACGGTTTA CGCTCAGACC CTCAACCTTG AAGGACCTGG AGTCAGTATT 
AAGGAGAAAA CATTGGTCAA ACCCACCGAG GTGCCAACCG AGCCTCCCAC CCCTCCCCCT 
CCATCAATAG TGCCTGATGC TCTTGACATG TGTAAAGGTG CGAAGGCTGA CTTGGTCTTC 
CTCATCGACG GCTCATGGAG TATTGGAGAT GACAGCTTTG CCAAAGTCCT CCAGTTTGTC 
ACTAGTGTGA CAGCAGCTTT TGATGTTGTT AGCCCAACTG GAATGCAGGT TTCATTTGTG 
CAGTATAGCG ATGATGCTAA GACTGAGTTC AAACTGAACA CCTACCACAA CAAGGGCATT 
GTCCTATCTG CACTGAAGTC AATCCGCTAC AGGGGAGGCA ACACTAAGAC TGGCATTGCT 
CTGAAGCACG TCTATGAAAA GGTACTAACG TCCGACAATG GCATGAGGAG GAATGTCCCG 
AAGGTGCTGG TTGTTGTCAC AGATGGTCGT TCTCAAGATG AAGTCAAGAA GAGCGCAGAA 
AAACTTCAGC ATTCTGGCTA TAGTGTGTTT GTGGTAGGAG TGGCTGATGT GAGCATGAGA 
GAACTGGCAG TTATCGGCAG CAAGCCCACC GAAAGACACG TGTTTGTCGT TGACGATTAC 
GACGCTTTTT CCAAAATCCA GGACAACTTG ATCACATTCA TCTGTGAAAC GGCTACCTCC 
ACTTGCCCTC TCATCTACAT CAATGGATAC ACCACACCTG GATTTAGGAT GCTGGAAGCT 
TTCAACATCA CAGACCGAAC ATTTGCTGGC ATGAATGGTG TCTCCATGGA GCCAGGTTCT 
TTCAACAGCT TCATTGCCTA CAGGCTGCAC AAAGATGCCT TCCTAAACCA GCCCACAAAG 
GAAATCCACT CCGACGGCCT TCCTCCATCC TACACTATAA TCCTGCTCCT CCGCCTGCTG 
CCTGATACAC CCTCTGAACC TTTTGATATC TGGCAGATCT CTGACAAAAA CAACAACCCT 
GAAGTTGGAG TCACTATGAA TCCTTCCAGC AAAACACTCA CGTTCTACAA CAAGGACACC 
CGGGGAGAGA TCCAGAGAGC CGTGTTCAAT GAGGAGCAAG TGAAACGGGT TTTCCACGGA 
AGCTTCCACA AGCTCCACAT TACCGTCTCC CCAGAGAAGG TCAAGCTCAA CGTGGACTGC 
CAAGAGGTGG CAGAGAAGCC AATCAAGGAG GCCAACAACA TCACGCTGGA GGGCTATGAA 
GTGCTTGGCA AGATGGTCAA ATCAGGAGGT GGAAAGAGGC AGTCCGCTAC ATTCCAGCTC 
CAGATGTTTG ACATTGTCTG CAGCTTGAGT TGGATCAGCC GTGACAAATG CTGTGACCTG 
CCTGCTACGA GAATTGAGTC CAAATGTCCT GCTCTTCCCC ACTCCTGCAC ATGCACCCAA 
GACAGTGTTG GGCCCCAGGG GCCTCCTGGG CCTTCGGGCA GCCCAGGGTC TAAAGGACCC 
AGAGGAGACA GGGGAGACCC AGGCAAACCT GGTGCAATGG GTCCACGAGG TGAGCCAGGA 
CTCCAAGGTG CAATGGGCCC ACCAGGCCCA CAAGGCCCTA ATGGACTGTC TATCCCCGGA 
GAACCGGGTC GCCAAGGACC AAAGGGAGAT CGTGGAGACT CAGGCCTGCC TGGGCTGCAA 
GGTCCTCCTG GGCCGCGTGG TCCTATGGGT CCTGTCGGAC AAAGTGGAGC ACGGGGGCCA 
CCAGGAAAGG AAGGATCATC TGGACCCAGA GGCCCACCAG GGCCCATGGG AAGTCCAGGA 
AATCCAGGTG TACCTGGTAT GTCTGGAAAA CCAGGAAAGT CAGGAGAACC AGGAAGTCCA 
GGACCTGTTG GCCTTAAAGG AGAAAAGGGA GAGCGGGGAG ACTTTGCATC CCAAAATATG 
ATGCGCTCCA TTGCCAGACA AGTTTGTGAA CAGCTCGTGA GCAGTCAGAT GAGCAGAATT 
GACGGGATGC TCAATCAAAT TCCTTCTGGA TATCGTAATG GCAATCCAGG CCCGGCTGGT 
CCTCCTGGTC CCCCTGGCAA CCAAGGACCT CGTGGAGAGC CCGGACAACC TGGAAAATCT 
GGTTTCCCTG GAAGCCCAGG ATTACCTGGA AATCAAGGAG AAAGAGGGTT ACCAGGAGAG 
AGGGGGGAGA GAGGACCTCC AGGAGCAGAT ATCAGAGGAC AAAGAGGTCC ACCTGGACCA 
GCAGGTCCAC CAGGGGAGTC TAGAACAGGT CCCCCAGGTT CTCCTGGCTC TGCTGGATCC 
CGTGGCCCCC CAGGTCGTCA AGGTAATCCA GGTGTGAGAG GACCACCAGG ACCCCCTGGA 
TACTGTGACT CCTCTCAGTG TGTTGGTATT CCTTACAATG GACAAGGATA CACAGCTCCA 
CGCTACCAGC CTGAACCTGA TGTAGCGTCT ATACCTGTGG AGCCAACTGA AGAGTTTGAG 
ACAGTACAAT CATCAGGTTA TACAAGGAAC CAACGGGGAA AGAGATCGCT ACCACTAGAC 
AATTCAGGAG CATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488805.1
CDS	230..9547
Translation	MKIRLSLATAAFLVGFLASIDAQVEPPSDLKFKILNENTVEMSWLKPSSRIEGFRIQVASDADEPSRDFTLDAYSTSTSITNLTPDVDYSVSISSYYGSEESISIFGQLTIQSSNTTRPRPTSDALKCSVSAIADLVFLVDGSWSVGRENFKHIRSFIGSLAGAFDIGEDKTRVAVVQYSTDTRTEFPLNRYTRRGDLLQAINSLPYKGGNTMTGDAIDYLIKNIFTEAAGSRKAFPKVAMIITDGKSQDPVEEHARRLRNIGVEIFVLGIKGADEDELREIASTPHAKHVYNVPNFDLIQEVQKRIITEVCSGVDDQLTSLVSGEEMVEAPSNLQVTEIASKSMRLTWDASLGDITGYKLTLIPLVPGMKRQELYMGATQTSINVRDLSPETEYDISLFALKGLTPSEPLTDLAKTQPVKVLTECSMDVDVQADVVLLVDGSYSIGLQNFAKVREFLEVLVKSFDIGPDKIQISLVQYSRDPHTEFYLNTHHDINAVVKAVRNFPYRGGSTNTGKAMTYVREKIFVPTRGARQNVPRVMVLITDGKSSDSFRVAATKLRNIDVEIFAVGVKDAVRSELEAIANEPADTHVYEVEDFDAFQRISKELTQSICLRIEQELLKIKKKNLLPPSNLQFSEITSRSFRATWVAPAASVMSYLVRYRKAEDITGDYISLAVPGQTTTTVLLYLTPITTYEVNVYAEYDKGDSFALTGQETTLEEQGTVENLRVTEETTNSFRVSWQAAPGSVIRYRLSYVPLSGMGEILEAQTLGPETTIVLQELFPITTYRVSVSPEYAAGVGDELQVDGTTKEVIGSPRDLRVFDETINTMKVAWQAAPGNVVQYHVTYKLAEGGDINMELVQGDITQALLKNLQPATEYELFVTAHYSTGQGDPLMGAGTTLEELGSPRDLETRDVTDTSFAVSWTAAPGNVRQYRIKWKSMFSEETGEKTVAGDTTTADLDGLTPETRYQVSVFARYGHGEGQPLVGEETTDISANGRVIVVSDETEKSMKVTWQPAPGNVLNYRVTYKPQSGGRQLAAKVPGGNTSTVLRRLTSLTTYDITVVPVYRFGEGKIRQGEGTTLTPYKGPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVSFHPAEDDIDLRELLVSPHDNTVVLEELRAGTKYTVSVFGMFDGGESMPLAGEENTTYSDDPDSSPVDVPKMSCKTKAQADIVLLVDGSWSIGRLNFKTIRSFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHQTRESLLEAVAKLPYKGGNTMTGLALYYILQNNFQESVGMRPGARKIGVLITDGKSQDEIIASSQSLRDAGIELYAIGVKNADENELRAIASDPDEIHMYNVADFSFLLDIVDTLSDNLCNSVTGSDNTPGSPTNLVTSEVTHQSFRATWTGPEGPVEKYRVEYMTVSGAPQEVLVDGTVTTVVLRGLDPLTKYLVNVYSVIGEESSEPLKGTETTLPLSAVREMSVYDEQMTTMRARWKAVQGATGYLLTYRATNATQPTVEQEMRVAAGTTDVQLVKLTPNTAYSLSLYALYGLSASEALLRQGVTLPMPPAGELNVRDVTHSTMVLYWDAAPGAVRKYIITYTPEDGETKELEVGGRITTKLLENLISQTEYSLTVTPVYDEGRGQAMLGSGITDVVPAPKNLRFSEITQTSFRATWEHGAPDVAMYRIAWRRKGETINEGEEILSEEETTFVLENLDPDTPYDVSVTAIYPDESESEDLLGTERTLPNAPPTNLVVFNETTTTLNARWTPPLGRVQNYRITYVPTSGGQSQTTMVGAKKNTVLLPKLTPDTEYSIGVVAVYANGVSRELNGLGKTKPLGGVRNLRVTNPTTSTLDVNWEPAEGNVRQYRVFYVPAAGGEQEMIQVSGSTYKAVLKNLQSDTVYTVTVTPVYSTGEGKPMSENGKTLERSPARNIQVFNPTTNTLNVRWEAATGPVQQYRVVYAPLTGVRPTESILVPGTTTTALLQQLVPDTAYNVGVVALYSDGEGTTVSDQGKTLPRGGPRNMRVYDPTTSTLSVNWEHADGPVLQYRISYAPTTGDPIEEFTTVQGNRNNVVLQNLDPDTPYNIRVTAMYAEGAGGELEGDGRTVGMLGPRNLRVSDEWYTRFRVSWDPAPSAVTGYKLIYQPAGSYDSSEVFVGDVTTYQLQNLKPGTTYDLTVLAQYRTGISGPLNGQGTTLYLNVTGLTSYNVGYDSFCIQWVPHRAATSYRLKVNPFESFKGGAQEITVQGPESKYCFDNLTPDTLYNATVYAQTLNLEGPGVSIKEKTLVKPTEVPTEPPTPPPPSIVPDALDMCKGAKADLVFLIDGSWSIGDDSFAKVLQFVTSVTAAFDVVSPTGMQVSFVQYSDDAKTEFKLNTYHNKGIVLSALKSIRYRGGNTKTGIALKHVYEKVLTSDNGMRRNVPKVLVVVTDGRSQDEVKKSAEKLQHSGYSVFVVGVADVSMRELAVIGSKPTERHVFVVDDYDAFSKIQDNLITFICETATSTCPLIYINGYTTPGFRMLEAFNITDRTFAGMNGVSMEPGSFNSFIAYRLHKDAFLNQPTKEIHSDGLPPSYTIILLLRLLPDTPSEPFDIWQISDKNNNPEVGVTMNPSSKTLTFYNKDTRGEIQRAVFNEEQVKRVFHGSFHKLHITVSPEKVKLNVDCQEVAEKPIKEANNITLEGYEVLGKMVKSGGGKRQSATFQLQMFDIVCSLSWISRDKCCDLPATRIESKCPALPHSCTCTQDSVGPQGPPGPSGSPGSKGPRGDRGDPGKPGAMGPRGEPGLQGAMGPPGPQGPNGLSIPGEPGRQGPKGDRGDSGLPGLQGPPGPRGPMGPVGQSGARGPPGKEGSSGPRGPPGPMGSPGNPGVPGMSGKPGKSGEPGSPGPVGLKGEKGERGDFASQNMMRSIARQVCEQLVSSQMSRIDGMLNQIPSGYRNGNPGPAGPPGPPGNQGPRGEPGQPGKSGFPGSPGLPGNQGERGLPGERGERGPPGADIRGQRGPPGPAGPPGESRTGPPGSPGSAGSRGPPGRQGNPGVRGPPGPPGYCDSSQCVGIPYNGQGYTAPRYQPEPDVASIPVEPTEEFETVQSSGYTRNQRGKRSLPLDNSGAS

Target ORF information:

RefSeq Version	XM_011490503.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490503.3

ORF Insert Sequence:

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TACCAAATGC ACCTCCCACC AATTTGGTTG TTTTCAATGA GACCACCACC 
ACCCTGAATG CTAGGTGGAC TCCACCTCTG GGTCGTGTCC AGAACTACAG GATCACTTAT 
GTCCCCACAT CTGGAGGCCA GAGTCAGACT ACCATGGTTG GAGCCAAGAA AAACACAGTC 
CTGCTTCCAA AGCTGACCCC TGACACAGAG TACTCCATCG GTGTTGTGGC GGTGTACGCC 
AATGGAGTCA GCAGGGAGCT GAATGGCCTT GGAAAAACCA AACCTTTGGG TGGTGTTAGA 
AACCTGCGTG TGACAAACCC CACCACGAGC ACGTTGGATG TGAACTGGGA ACCTGCTGAG 
GGTAATGTGC GCCAGTATAG GGTCTTCTAT GTTCCTGCAG CTGGAGGAGA GCAGGAGATG 
ATTCAGGTCT CCGGAAGCAC CTACAAAGCA GTTCTGAAGA ACCTGCAGTC GGACACTGTC 
TACACTGTCA CAGTGACTCC TGTTTATTCT ACTGGAGAGG GGAAGCCCAT GTCTGAAAAC 
GGCAAAACAT TGGAGCGCAG TCCTGCTAGG AATATCCAGG TTTTCAACCC CACAACTAAC 
ACTTTGAATG TCCGCTGGGA AGCAGCCACC GGGCCGGTCC AGCAGTACAG GGTGGTCTAT 
GCTCCTTTGA CTGGTGTGAG GCCAACGGAG TCGATTTTGG TCCCCGGCAC CACAACCACA 
GCACTGCTGC AGCAGCTGGT CCCAGACACA GCCTACAATG TGGGAGTTGT TGCCTTGTAC 
AGCGATGGGG AAGGAACCAC TGTCAGCGAT CAGGGAAAGA CTTTGCCCCG GGGTGGTCCC 
AGAAACATGC GTGTGTACGA TCCCACCACC AGTACGCTGT CTGTTAACTG GGAACACGCT 
GACGGTCCTG TTCTTCAGTA CCGCATCAGC TATGCCCCAA CAACAGGAGA CCCTATCGAG 
GAATTTACCA CAGTTCAAGG GAACAGGAAC AATGTGGTTC TTCAGAATCT GGATCCAGAC 
ACTCCCTACA ATATCAGAGT GACTGCTATG TATGCTGAAG GAGCGGGAGG AGAACTGGAG 
GGAGATGGAC GTACAGTTGG AATGCTCGGC CCCAGAAACC TGCGTGTATC TGATGAATGG 
TACACTCGCT TCAGGGTGTC CTGGGACCCC GCCCCCTCCG CAGTCACTGG ATACAAACTC 
ATCTACCAGC CTGCGGGGTC TTATGACTCA TCTGAGGTGT TTGTTGGAGA TGTGACCACC 
TATCAGCTGC AAAACTTGAA GCCTGGAACT ACCTATGACT TGACGGTCCT AGCACAGTAC 
AGAACGGGCA TCAGTGGTCC TCTAAATGGT CAAGGCACCA CATTGTACCT CAATGTGACA 
GGCCTAACCT CCTACAATGT TGGCTATGAC TCCTTTTGCA TCCAGTGGGT CCCTCACCGA 
GCAGCCACGT CGTACAGACT TAAAGTCAAC CCTTTTGAGT CCTTCAAGGG TGGAGCTCAA 
GAAATCACAG TTCAGGGCCC AGAGAGTAAA TACTGCTTTG ACAACCTGAC CCCTGACACT 
CTTTACAACG CTACGGTTTA CGCTCAGACC CTCAACCTTG AAGGACCTGG AGTCAGTATT 
AAGGAGAAAA CATTGGTCAA ACCCACCGAG GTGCCAACCG AGCCTCCCAC CCCTCCCCCT 
CCATCAATAG TGCCTGATGC TCTTGACATG TGTAAAGGTG CGAAGGCTGA CTTGGTCTTC 
CTCATCGACG GCTCATGGAG TATTGGAGAT GACAGCTTTG CCAAAGTCCT CCAGTTTGTC 
ACTAGTGTGA CAGCAGCTTT TGATGTTGTT AGCCCAACTG GAATGCAGGT TTCATTTGTG 
CAGTATAGCG ATGATGCTAA GACTGAGTTC AAACTGAACA CCTACCACAA CAAGGGCATT 
GTCCTATCTG CACTGAAGTC AATCCGCTAC AGGGGAGGCA ACACTAAGAC TGGCATTGCT 
CTGAAGCACG TCTATGAAAA GGTACTAACG TCCGACAATG GCATGAGGAG GAATGTCCCG 
AAGGTGCTGG TTGTTGTCAC AGATGGTCGT TCTCAAGATG AAGTCAAGAA GAGCGCAGAA 
AAACTTCAGC ATTCTGGCTA TAGTGTGTTT GTGGTAGGAG TGGCTGATGT GAGCATGAGA 
GAACTGGCAG TTATCGGCAG CAAGCCCACC GAAAGACACG TGTTTGTCGT TGACGATTAC 
GACGCTTTTT CCAAAATCCA GGACAACTTG ATCACATTCA TCTGTGAAAC GGCTACCTCC 
ACTTGCCCTC TCATCTACAT CAATGGATAC ACCACACCTG GATTTAGGAT GCTGGAAGCT 
TTCAACATCA CAGACCGAAC ATTTGCTGGC ATGAATGGTG TCTCCATGGA GCCAGGTTCT 
TTCAACAGCT TCATTGCCTA CAGGCTGCAC AAAGATGCCT TCCTAAACCA GCCCACAAAG 
GAAATCCACT CCGACGGCCT TCCTCCATCC TACACTATAA TCCTGCTCCT CCGCCTGCTG 
CCTGATACAC CCTCTGAACC TTTTGATATC TGGCAGATCT CTGACAAAAA CAACAACCCT 
GAAGTTGGAG TCACTATGAA TCCTTCCAGC AAAACACTCA CGTTCTACAA CAAGGACACC 
CGGGGAGAGA TCCAGAGAGC CGTGTTCAAT GAGGAGCAAG TGAAACGGGT TTTCCACGGA 
AGCTTCCACA AGCTCCACAT TACCGTCTCC CCAGAGAAGG TCAAGCTCAA CGTGGACTGC 
CAAGAGGTGG CAGAGAAGCC AATCAAGGAG GCCAACAACA TCACGCTGGA GGGCTATGAA 
GTGCTTGGCA AGATGGTCAA ATCAGGAGGT GGAAAGAGGC AGTCCGCTAC ATTCCAGCTC 
CAGATGTTTG ACATTGTCTG CAGCTTGAGT TGGATCAGCC GTGACAAATG CTGTGACCTG 
CCTGCTACGA GAATTGAGTC CAAATGTCCT GCTCTTCCCC ACTCCTGCAC ATGCACCCAA 
GACAGTGTTG GGCCCCAGGG GCCTCCTGGG CCTTCGGGCA GCCCAGGGTC TAAAGGACCC 
AGAGGAGACA GGGGAGACCC AGGCAAACCT GGTGCAATGG GTCCACGAGG TGAGCCAGGA 
CTCCAAGGTG CAATGGGCCC ACCAGGCCCA CAAGGCCCTA ATGGACTGTC TATCCCCGGA 
GAACCGGGTC GCCAAGGACC AAAGGGAGAT CGTGGAGACT CAGGCCTGCC TGGGCTGCAA 
GGTCCTCCTG GGCCGCGTGG TCCTATGGGT CCTGTCGGAC AAAGTGGAGC ACGGGGGCCA 
CCAGGAAAGG AAGGATCATC TGGACCCAGA GGCCCACCAG GGCCCATGGG AAGTCCAGGA 
AATCCAGGTG TACCTGGTAT GTCTGGAAAA CCAGGAAAGT CAGGAGAACC AGGAAGTCCA 
GGACCTGTTG GCCTTAAAGG AGAAAAGGGA GAGCGGGGAG ACTTTGCATC CCAAAATATG 
ATGCGCTCCA TTGCCAGACA AGTTTGTGAA CAGCTCGTGA GCAGTCAGAT GAGCAGAATT 
GACGGGATGC TCAATCAAAT TCCTTCTGGA TATCGTAATG GCAATCCAGG CCCGGCTGGT 
CCTCCTGGTC CCCCTGGCAA CCAAGGACCT CGTGGAGAGC CCGGACAACC TGGAAAATCT 
GGTTTCCCTG GAAGCCCAGG ATTACCTGGA AATCAAGGAG AAAGAGGGTT ACCAGGAGAG 
AGGGGGGAGA GAGGACCTCC AGGAGCAGAT ATCAGAGGAC AAAGAGGTCC ACCTGGACCA 
GCAGGTCCAC CAGGGGAGTC TAGAACAGGT CCCCCAGGTT CTCCTGGCTC TGCTGGATCC 
CGTGGCCCCC CAGGTCGTCA AGGTAATCCA GGTGTGAGAG GACCACCAGG ACCCCCTGGA 
TACTGTGACT CCTCTCAGTG TGTTGGTATT CCTTACAATG GACAAGGATA CACAGCTCCA 
CGCTACCAGC CTGAACCTGA TGTAGCGTCT ATACCTGTGG AGCCAACTGA AGAGTTTGAG 
ACAGTACAAT CATCAGGTTA TACAAGGAAC CAACGGGGAA AGAGATCGCT ACCACTAGAC 
AATTCAGGAG CATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col12a1 ( XM_011490503.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488805.1 Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X2, mRNA.

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CloneID	OJa24262
Clone ID Related Accession (Same CDS sequence)	XM_011490503.3 , XM_011490503.2
Accession Version	XM_011490503.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	collagen alpha-1(XII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011490503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TACCAAATGC ACCTCCCACC AATTTGGTTG TTTTCAATGA GACCACCACC 
ACCCTGAATG CTAGGTGGAC TCCACCTCTG GGTCGTGTCC AGAACTACAG GATCACTTAT 
GTCCCCACAT CTGGAGGCCA GAGTCAGACT ACCATGGTTG GAGCCAAGAA AAACACAGTC 
CTGCTTCCAA AGCTGACCCC TGACACAGAG TACTCCATCG GTGTTGTGGC GGTGTACGCC 
AATGGAGTCA GCAGGGAGCT GAATGGCCTT GGAAAAACCA AACCTTTGGG TGGTGTTAGA 
AACCTGCGTG TGACAAACCC CACCACGAGC ACGTTGGATG TGAACTGGGA ACCTGCTGAG 
GGTAATGTGC GCCAGTATAG GGTCTTCTAT GTTCCTGCAG CTGGAGGAGA GCAGGAGATG 
ATTCAGGTCT CCGGAAGCAC CTACAAAGCA GTTCTGAAGA ACCTGCAGTC GGACACTGTC 
TACACTGTCA CAGTGACTCC TGTTTATTCT ACTGGAGAGG GGAAGCCCAT GTCTGAAAAC 
GGCAAAACAT TGGAGCGCAG TCCTGCTAGG AATATCCAGG TTTTCAACCC CACAACTAAC 
ACTTTGAATG TCCGCTGGGA AGCAGCCACC GGGCCGGTCC AGCAGTACAG GGTGGTCTAT 
GCTCCTTTGA CTGGTGTGAG GCCAACGGAG TCGATTTTGG TCCCCGGCAC CACAACCACA 
GCACTGCTGC AGCAGCTGGT CCCAGACACA GCCTACAATG TGGGAGTTGT TGCCTTGTAC 
AGCGATGGGG AAGGAACCAC TGTCAGCGAT CAGGGAAAGA CTTTGCCCCG GGGTGGTCCC 
AGAAACATGC GTGTGTACGA TCCCACCACC AGTACGCTGT CTGTTAACTG GGAACACGCT 
GACGGTCCTG TTCTTCAGTA CCGCATCAGC TATGCCCCAA CAACAGGAGA CCCTATCGAG 
GAATTTACCA CAGTTCAAGG GAACAGGAAC AATGTGGTTC TTCAGAATCT GGATCCAGAC 
ACTCCCTACA ATATCAGAGT GACTGCTATG TATGCTGAAG GAGCGGGAGG AGAACTGGAG 
GGAGATGGAC GTACAGTTGG AATGCTCGGC CCCAGAAACC TGCGTGTATC TGATGAATGG 
TACACTCGCT TCAGGGTGTC CTGGGACCCC GCCCCCTCCG CAGTCACTGG ATACAAACTC 
ATCTACCAGC CTGCGGGGTC TTATGACTCA TCTGAGGTGT TTGTTGGAGA TGTGACCACC 
TATCAGCTGC AAAACTTGAA GCCTGGAACT ACCTATGACT TGACGGTCCT AGCACAGTAC 
AGAACGGGCA TCAGTGGTCC TCTAAATGGT CAAGGCACCA CATTGTACCT CAATGTGACA 
GGCCTAACCT CCTACAATGT TGGCTATGAC TCCTTTTGCA TCCAGTGGGT CCCTCACCGA 
GCAGCCACGT CGTACAGACT TAAAGTCAAC CCTTTTGAGT CCTTCAAGGG TGGAGCTCAA 
GAAATCACAG TTCAGGGCCC AGAGAGTAAA TACTGCTTTG ACAACCTGAC CCCTGACACT 
CTTTACAACG CTACGGTTTA CGCTCAGACC CTCAACCTTG AAGGACCTGG AGTCAGTATT 
AAGGAGAAAA CATTGGTCAA ACCCACCGAG GTGCCAACCG AGCCTCCCAC CCCTCCCCCT 
CCATCAATAG TGCCTGATGC TCTTGACATG TGTAAAGGTG CGAAGGCTGA CTTGGTCTTC 
CTCATCGACG GCTCATGGAG TATTGGAGAT GACAGCTTTG CCAAAGTCCT CCAGTTTGTC 
ACTAGTGTGA CAGCAGCTTT TGATGTTGTT AGCCCAACTG GAATGCAGGT TTCATTTGTG 
CAGTATAGCG ATGATGCTAA GACTGAGTTC AAACTGAACA CCTACCACAA CAAGGGCATT 
GTCCTATCTG CACTGAAGTC AATCCGCTAC AGGGGAGGCA ACACTAAGAC TGGCATTGCT 
CTGAAGCACG TCTATGAAAA GGTACTAACG TCCGACAATG GCATGAGGAG GAATGTCCCG 
AAGGTGCTGG TTGTTGTCAC AGATGGTCGT TCTCAAGATG AAGTCAAGAA GAGCGCAGAA 
AAACTTCAGC ATTCTGGCTA TAGTGTGTTT GTGGTAGGAG TGGCTGATGT GAGCATGAGA 
GAACTGGCAG TTATCGGCAG CAAGCCCACC GAAAGACACG TGTTTGTCGT TGACGATTAC 
GACGCTTTTT CCAAAATCCA GGACAACTTG ATCACATTCA TCTGTGAAAC GGCTACCTCC 
ACTTGCCCTC TCATCTACAT CAATGGATAC ACCACACCTG GATTTAGGAT GCTGGAAGCT 
TTCAACATCA CAGACCGAAC ATTTGCTGGC ATGAATGGTG TCTCCATGGA GCCAGGTTCT 
TTCAACAGCT TCATTGCCTA CAGGCTGCAC AAAGATGCCT TCCTAAACCA GCCCACAAAG 
GAAATCCACT CCGACGGCCT TCCTCCATCC TACACTATAA TCCTGCTCCT CCGCCTGCTG 
CCTGATACAC CCTCTGAACC TTTTGATATC TGGCAGATCT CTGACAAAAA CAACAACCCT 
GAAGTTGGAG TCACTATGAA TCCTTCCAGC AAAACACTCA CGTTCTACAA CAAGGACACC 
CGGGGAGAGA TCCAGAGAGC CGTGTTCAAT GAGGAGCAAG TGAAACGGGT TTTCCACGGA 
AGCTTCCACA AGCTCCACAT TACCGTCTCC CCAGAGAAGG TCAAGCTCAA CGTGGACTGC 
CAAGAGGTGG CAGAGAAGCC AATCAAGGAG GCCAACAACA TCACGCTGGA GGGCTATGAA 
GTGCTTGGCA AGATGGTCAA ATCAGGAGGT GGAAAGAGGC AGTCCGCTAC ATTCCAGCTC 
CAGATGTTTG ACATTGTCTG CAGCTTGAGT TGGATCAGCC GTGACAAATG CTGTGACCTG 
CCTGCTACGA GAATTGAGTC CAAATGTCCT GCTCTTCCCC ACTCCTGCAC ATGCACCCAA 
GACAGTGTTG GGCCCCAGGG GCCTCCTGGG CCTTCGGGCA GCCCAGGGTC TAAAGGACCC 
AGAGGAGACA GGGGAGACCC AGGCAAACCT GGTGCAATGG GTCCACGAGG TGAGCCAGGA 
CTCCAAGGTG CAATGGGCCC ACCAGGCCCA CAAGGCCCTA ATGGACTGTC TATCCCCGGA 
GAACCGGGTC GCCAAGGACC AAAGGGAGAT CGTGGAGACT CAGGCCTGCC TGGGCTGCAA 
GGTCCTCCTG GGCCGCGTGG TCCTATGGGT CCTGTCGGAC AAAGTGGAGC ACGGGGGCCA 
CCAGGAAAGG AAGGATCATC TGGACCCAGA GGCCCACCAG GGCCCATGGG AAGTCCAGGA 
AATCCAGGTG TACCTGGTAT GTCTGGAAAA CCAGGAAAGT CAGGAGAACC AGGAAGTCCA 
GGACCTGTTG GCCTTAAAGG AGAAAAGGGA GAGCGGGGAG ACTTTGCATC CCAAAATATG 
ATGCGCTCCA TTGCCAGACA AGTTTGTGAA CAGCTCGTGA GCAGTCAGAT GAGCAGAATT 
GACGGGATGC TCAATCAAAT TCCTTCTGGA TATCGTAATG GCAATCCAGG CCCGGCTGGT 
CCTCCTGGTC CCCCTGGCAA CCAAGGACCT CGTGGAGAGC CCGGACAACC TGGAAAATCT 
GGTTTCCCTG GAAGCCCAGG ATTACCTGGA AATCAAGGAG AAAGAGGGTT ACCAGGAGAG 
AGGGGGGAGA GAGGACCTCC AGGAGCAGAT ATCAGAGGAC AAAGAGGTCC ACCTGGACCA 
GCAGGTCCAC CAGGGGAGTC TAGAACAGGT CCCCCAGGTT CTCCTGGCTC TGCTGGATCC 
CGTGGCCCCC CAGGTCGTCA AGGTAATCCA GGTGTGAGAG GACCACCAGG ACCCCCTGGA 
TACTGTGACT CCTCTCAGTG TGTTGGTATT CCTTACAATG GACAAGGATA CACAGCTCCA 
CGCTACCAGC CTGAACCTGA TGTAGCGTCT ATACCTGTGG AGCCAACTGA AGAGTTTGAG 
ACAGTACAAT CATCAGGTTA TACAAGGAAC CAACGGGGAA AGAGATCGCT ACCACTAGAC 
AATTCAGGAG CATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488805.1
CDS	231..9548
Translation	MKIRLSLATAAFLVGFLASIDAQVEPPSDLKFKILNENTVEMSWLKPSSRIEGFRIQVASDADEPSRDFTLDAYSTSTSITNLTPDVDYSVSISSYYGSEESISIFGQLTIQSSNTTRPRPTSDALKCSVSAIADLVFLVDGSWSVGRENFKHIRSFIGSLAGAFDIGEDKTRVAVVQYSTDTRTEFPLNRYTRRGDLLQAINSLPYKGGNTMTGDAIDYLIKNIFTEAAGSRKAFPKVAMIITDGKSQDPVEEHARRLRNIGVEIFVLGIKGADEDELREIASTPHAKHVYNVPNFDLIQEVQKRIITEVCSGVDDQLTSLVSGEEMVEAPSNLQVTEIASKSMRLTWDASLGDITGYKLTLIPLVPGMKRQELYMGATQTSINVRDLSPETEYDISLFALKGLTPSEPLTDLAKTQPVKVLTECSMDVDVQADVVLLVDGSYSIGLQNFAKVREFLEVLVKSFDIGPDKIQISLVQYSRDPHTEFYLNTHHDINAVVKAVRNFPYRGGSTNTGKAMTYVREKIFVPTRGARQNVPRVMVLITDGKSSDSFRVAATKLRNIDVEIFAVGVKDAVRSELEAIANEPADTHVYEVEDFDAFQRISKELTQSICLRIEQELLKIKKKNLLPPSNLQFSEITSRSFRATWVAPAASVMSYLVRYRKAEDITGDYISLAVPGQTTTTVLLYLTPITTYEVNVYAEYDKGDSFALTGQETTLEEQGTVENLRVTEETTNSFRVSWQAAPGSVIRYRLSYVPLSGMGEILEAQTLGPETTIVLQELFPITTYRVSVSPEYAAGVGDELQVDGTTKEVIGSPRDLRVFDETINTMKVAWQAAPGNVVQYHVTYKLAEGGDINMELVQGDITQALLKNLQPATEYELFVTAHYSTGQGDPLMGAGTTLEELGSPRDLETRDVTDTSFAVSWTAAPGNVRQYRIKWKSMFSEETGEKTVAGDTTTADLDGLTPETRYQVSVFARYGHGEGQPLVGEETTDISANGRVIVVSDETEKSMKVTWQPAPGNVLNYRVTYKPQSGGRQLAAKVPGGNTSTVLRRLTSLTTYDITVVPVYRFGEGKIRQGEGTTLTPYKGPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVSFHPAEDDIDLRELLVSPHDNTVVLEELRAGTKYTVSVFGMFDGGESMPLAGEENTTYSDDPDSSPVDVPKMSCKTKAQADIVLLVDGSWSIGRLNFKTIRSFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHQTRESLLEAVAKLPYKGGNTMTGLALYYILQNNFQESVGMRPGARKIGVLITDGKSQDEIIASSQSLRDAGIELYAIGVKNADENELRAIASDPDEIHMYNVADFSFLLDIVDTLSDNLCNSVTGSDNTPGSPTNLVTSEVTHQSFRATWTGPEGPVEKYRVEYMTVSGAPQEVLVDGTVTTVVLRGLDPLTKYLVNVYSVIGEESSEPLKGTETTLPLSAVREMSVYDEQMTTMRARWKAVQGATGYLLTYRATNATQPTVEQEMRVAAGTTDVQLVKLTPNTAYSLSLYALYGLSASEALLRQGVTLPMPPAGELNVRDVTHSTMVLYWDAAPGAVRKYIITYTPEDGETKELEVGGRITTKLLENLISQTEYSLTVTPVYDEGRGQAMLGSGITDVVPAPKNLRFSEITQTSFRATWEHGAPDVAMYRIAWRRKGETINEGEEILSEEETTFVLENLDPDTPYDVSVTAIYPDESESEDLLGTERTLPNAPPTNLVVFNETTTTLNARWTPPLGRVQNYRITYVPTSGGQSQTTMVGAKKNTVLLPKLTPDTEYSIGVVAVYANGVSRELNGLGKTKPLGGVRNLRVTNPTTSTLDVNWEPAEGNVRQYRVFYVPAAGGEQEMIQVSGSTYKAVLKNLQSDTVYTVTVTPVYSTGEGKPMSENGKTLERSPARNIQVFNPTTNTLNVRWEAATGPVQQYRVVYAPLTGVRPTESILVPGTTTTALLQQLVPDTAYNVGVVALYSDGEGTTVSDQGKTLPRGGPRNMRVYDPTTSTLSVNWEHADGPVLQYRISYAPTTGDPIEEFTTVQGNRNNVVLQNLDPDTPYNIRVTAMYAEGAGGELEGDGRTVGMLGPRNLRVSDEWYTRFRVSWDPAPSAVTGYKLIYQPAGSYDSSEVFVGDVTTYQLQNLKPGTTYDLTVLAQYRTGISGPLNGQGTTLYLNVTGLTSYNVGYDSFCIQWVPHRAATSYRLKVNPFESFKGGAQEITVQGPESKYCFDNLTPDTLYNATVYAQTLNLEGPGVSIKEKTLVKPTEVPTEPPTPPPPSIVPDALDMCKGAKADLVFLIDGSWSIGDDSFAKVLQFVTSVTAAFDVVSPTGMQVSFVQYSDDAKTEFKLNTYHNKGIVLSALKSIRYRGGNTKTGIALKHVYEKVLTSDNGMRRNVPKVLVVVTDGRSQDEVKKSAEKLQHSGYSVFVVGVADVSMRELAVIGSKPTERHVFVVDDYDAFSKIQDNLITFICETATSTCPLIYINGYTTPGFRMLEAFNITDRTFAGMNGVSMEPGSFNSFIAYRLHKDAFLNQPTKEIHSDGLPPSYTIILLLRLLPDTPSEPFDIWQISDKNNNPEVGVTMNPSSKTLTFYNKDTRGEIQRAVFNEEQVKRVFHGSFHKLHITVSPEKVKLNVDCQEVAEKPIKEANNITLEGYEVLGKMVKSGGGKRQSATFQLQMFDIVCSLSWISRDKCCDLPATRIESKCPALPHSCTCTQDSVGPQGPPGPSGSPGSKGPRGDRGDPGKPGAMGPRGEPGLQGAMGPPGPQGPNGLSIPGEPGRQGPKGDRGDSGLPGLQGPPGPRGPMGPVGQSGARGPPGKEGSSGPRGPPGPMGSPGNPGVPGMSGKPGKSGEPGSPGPVGLKGEKGERGDFASQNMMRSIARQVCEQLVSSQMSRIDGMLNQIPSGYRNGNPGPAGPPGPPGNQGPRGEPGQPGKSGFPGSPGLPGNQGERGLPGERGERGPPGADIRGQRGPPGPAGPPGESRTGPPGSPGSAGSRGPPGRQGNPGVRGPPGPPGYCDSSQCVGIPYNGQGYTAPRYQPEPDVASIPVEPTEEFETVQSSGYTRNQRGKRSLPLDNSGAS

Target ORF information:

RefSeq Version	XM_011490503.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490503.2

ORF Insert Sequence:

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TACCAAATGC ACCTCCCACC AATTTGGTTG TTTTCAATGA GACCACCACC 
ACCCTGAATG CTAGGTGGAC TCCACCTCTG GGTCGTGTCC AGAACTACAG GATCACTTAT 
GTCCCCACAT CTGGAGGCCA GAGTCAGACT ACCATGGTTG GAGCCAAGAA AAACACAGTC 
CTGCTTCCAA AGCTGACCCC TGACACAGAG TACTCCATCG GTGTTGTGGC GGTGTACGCC 
AATGGAGTCA GCAGGGAGCT GAATGGCCTT GGAAAAACCA AACCTTTGGG TGGTGTTAGA 
AACCTGCGTG TGACAAACCC CACCACGAGC ACGTTGGATG TGAACTGGGA ACCTGCTGAG 
GGTAATGTGC GCCAGTATAG GGTCTTCTAT GTTCCTGCAG CTGGAGGAGA GCAGGAGATG 
ATTCAGGTCT CCGGAAGCAC CTACAAAGCA GTTCTGAAGA ACCTGCAGTC GGACACTGTC 
TACACTGTCA CAGTGACTCC TGTTTATTCT ACTGGAGAGG GGAAGCCCAT GTCTGAAAAC 
GGCAAAACAT TGGAGCGCAG TCCTGCTAGG AATATCCAGG TTTTCAACCC CACAACTAAC 
ACTTTGAATG TCCGCTGGGA AGCAGCCACC GGGCCGGTCC AGCAGTACAG GGTGGTCTAT 
GCTCCTTTGA CTGGTGTGAG GCCAACGGAG TCGATTTTGG TCCCCGGCAC CACAACCACA 
GCACTGCTGC AGCAGCTGGT CCCAGACACA GCCTACAATG TGGGAGTTGT TGCCTTGTAC 
AGCGATGGGG AAGGAACCAC TGTCAGCGAT CAGGGAAAGA CTTTGCCCCG GGGTGGTCCC 
AGAAACATGC GTGTGTACGA TCCCACCACC AGTACGCTGT CTGTTAACTG GGAACACGCT 
GACGGTCCTG TTCTTCAGTA CCGCATCAGC TATGCCCCAA CAACAGGAGA CCCTATCGAG 
GAATTTACCA CAGTTCAAGG GAACAGGAAC AATGTGGTTC TTCAGAATCT GGATCCAGAC 
ACTCCCTACA ATATCAGAGT GACTGCTATG TATGCTGAAG GAGCGGGAGG AGAACTGGAG 
GGAGATGGAC GTACAGTTGG AATGCTCGGC CCCAGAAACC TGCGTGTATC TGATGAATGG 
TACACTCGCT TCAGGGTGTC CTGGGACCCC GCCCCCTCCG CAGTCACTGG ATACAAACTC 
ATCTACCAGC CTGCGGGGTC TTATGACTCA TCTGAGGTGT TTGTTGGAGA TGTGACCACC 
TATCAGCTGC AAAACTTGAA GCCTGGAACT ACCTATGACT TGACGGTCCT AGCACAGTAC 
AGAACGGGCA TCAGTGGTCC TCTAAATGGT CAAGGCACCA CATTGTACCT CAATGTGACA 
GGCCTAACCT CCTACAATGT TGGCTATGAC TCCTTTTGCA TCCAGTGGGT CCCTCACCGA 
GCAGCCACGT CGTACAGACT TAAAGTCAAC CCTTTTGAGT CCTTCAAGGG TGGAGCTCAA 
GAAATCACAG TTCAGGGCCC AGAGAGTAAA TACTGCTTTG ACAACCTGAC CCCTGACACT 
CTTTACAACG CTACGGTTTA CGCTCAGACC CTCAACCTTG AAGGACCTGG AGTCAGTATT 
AAGGAGAAAA CATTGGTCAA ACCCACCGAG GTGCCAACCG AGCCTCCCAC CCCTCCCCCT 
CCATCAATAG TGCCTGATGC TCTTGACATG TGTAAAGGTG CGAAGGCTGA CTTGGTCTTC 
CTCATCGACG GCTCATGGAG TATTGGAGAT GACAGCTTTG CCAAAGTCCT CCAGTTTGTC 
ACTAGTGTGA CAGCAGCTTT TGATGTTGTT AGCCCAACTG GAATGCAGGT TTCATTTGTG 
CAGTATAGCG ATGATGCTAA GACTGAGTTC AAACTGAACA CCTACCACAA CAAGGGCATT 
GTCCTATCTG CACTGAAGTC AATCCGCTAC AGGGGAGGCA ACACTAAGAC TGGCATTGCT 
CTGAAGCACG TCTATGAAAA GGTACTAACG TCCGACAATG GCATGAGGAG GAATGTCCCG 
AAGGTGCTGG TTGTTGTCAC AGATGGTCGT TCTCAAGATG AAGTCAAGAA GAGCGCAGAA 
AAACTTCAGC ATTCTGGCTA TAGTGTGTTT GTGGTAGGAG TGGCTGATGT GAGCATGAGA 
GAACTGGCAG TTATCGGCAG CAAGCCCACC GAAAGACACG TGTTTGTCGT TGACGATTAC 
GACGCTTTTT CCAAAATCCA GGACAACTTG ATCACATTCA TCTGTGAAAC GGCTACCTCC 
ACTTGCCCTC TCATCTACAT CAATGGATAC ACCACACCTG GATTTAGGAT GCTGGAAGCT 
TTCAACATCA CAGACCGAAC ATTTGCTGGC ATGAATGGTG TCTCCATGGA GCCAGGTTCT 
TTCAACAGCT TCATTGCCTA CAGGCTGCAC AAAGATGCCT TCCTAAACCA GCCCACAAAG 
GAAATCCACT CCGACGGCCT TCCTCCATCC TACACTATAA TCCTGCTCCT CCGCCTGCTG 
CCTGATACAC CCTCTGAACC TTTTGATATC TGGCAGATCT CTGACAAAAA CAACAACCCT 
GAAGTTGGAG TCACTATGAA TCCTTCCAGC AAAACACTCA CGTTCTACAA CAAGGACACC 
CGGGGAGAGA TCCAGAGAGC CGTGTTCAAT GAGGAGCAAG TGAAACGGGT TTTCCACGGA 
AGCTTCCACA AGCTCCACAT TACCGTCTCC CCAGAGAAGG TCAAGCTCAA CGTGGACTGC 
CAAGAGGTGG CAGAGAAGCC AATCAAGGAG GCCAACAACA TCACGCTGGA GGGCTATGAA 
GTGCTTGGCA AGATGGTCAA ATCAGGAGGT GGAAAGAGGC AGTCCGCTAC ATTCCAGCTC 
CAGATGTTTG ACATTGTCTG CAGCTTGAGT TGGATCAGCC GTGACAAATG CTGTGACCTG 
CCTGCTACGA GAATTGAGTC CAAATGTCCT GCTCTTCCCC ACTCCTGCAC ATGCACCCAA 
GACAGTGTTG GGCCCCAGGG GCCTCCTGGG CCTTCGGGCA GCCCAGGGTC TAAAGGACCC 
AGAGGAGACA GGGGAGACCC AGGCAAACCT GGTGCAATGG GTCCACGAGG TGAGCCAGGA 
CTCCAAGGTG CAATGGGCCC ACCAGGCCCA CAAGGCCCTA ATGGACTGTC TATCCCCGGA 
GAACCGGGTC GCCAAGGACC AAAGGGAGAT CGTGGAGACT CAGGCCTGCC TGGGCTGCAA 
GGTCCTCCTG GGCCGCGTGG TCCTATGGGT CCTGTCGGAC AAAGTGGAGC ACGGGGGCCA 
CCAGGAAAGG AAGGATCATC TGGACCCAGA GGCCCACCAG GGCCCATGGG AAGTCCAGGA 
AATCCAGGTG TACCTGGTAT GTCTGGAAAA CCAGGAAAGT CAGGAGAACC AGGAAGTCCA 
GGACCTGTTG GCCTTAAAGG AGAAAAGGGA GAGCGGGGAG ACTTTGCATC CCAAAATATG 
ATGCGCTCCA TTGCCAGACA AGTTTGTGAA CAGCTCGTGA GCAGTCAGAT GAGCAGAATT 
GACGGGATGC TCAATCAAAT TCCTTCTGGA TATCGTAATG GCAATCCAGG CCCGGCTGGT 
CCTCCTGGTC CCCCTGGCAA CCAAGGACCT CGTGGAGAGC CCGGACAACC TGGAAAATCT 
GGTTTCCCTG GAAGCCCAGG ATTACCTGGA AATCAAGGAG AAAGAGGGTT ACCAGGAGAG 
AGGGGGGAGA GAGGACCTCC AGGAGCAGAT ATCAGAGGAC AAAGAGGTCC ACCTGGACCA 
GCAGGTCCAC CAGGGGAGTC TAGAACAGGT CCCCCAGGTT CTCCTGGCTC TGCTGGATCC 
CGTGGCCCCC CAGGTCGTCA AGGTAATCCA GGTGTGAGAG GACCACCAGG ACCCCCTGGA 
TACTGTGACT CCTCTCAGTG TGTTGGTATT CCTTACAATG GACAAGGATA CACAGCTCCA 
CGCTACCAGC CTGAACCTGA TGTAGCGTCT ATACCTGTGG AGCCAACTGA AGAGTTTGAG 
ACAGTACAAT CATCAGGTTA TACAAGGAAC CAACGGGGAA AGAGATCGCT ACCACTAGAC 
AATTCAGGAG CATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col12a1 ( XM_011490504.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488806.1 Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X3, mRNA.

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CloneID	OJa24263
Clone ID Related Accession (Same CDS sequence)	XM_011490504.3 , XM_011490504.2
Accession Version	XM_011490504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	collagen alpha-1(XII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011490504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TGTCCAAGTC AGATGGTAGA CCGATACCAA ATGCACCTCC CACCAATTTG 
GTTGTTTTCA ATGAGACCAC CACCACCCTG AATGCTAGGT GGACTCCACC TCTGGGTCGT 
GTCCAGAACT ACAGGATCAC TTATGTCCCC ACATCTGGAG GCCAGAGTCA GACTACCATG 
GTTGGAGCCA AGAAAAACAC AGTCCTGCTT CCAAAGCTGA CCCCTGACAC AGAGTACTCC 
ATCGGTGTTG TGGCGGTGTA CGCCAATGGA GTCAGCAGGG AGCTGAATGG CCTTGGAAAA 
ACCAAACCTT TGGGTGGTGT TAGAAACCTG CGTGTGACAA ACCCCACCAC GAGCACGTTG 
GATGTGAACT GGGAACCTGC TGAGGGTAAT GTGCGCCAGT ATAGGGTCTT CTATGTTCCT 
GCAGCTGGAG GAGAGCAGGA GATGATTCAG GTCTCCGGAA GCACCTACAA AGCAGTTCTG 
AAGAACCTGC AGTCGGACAC TGTCTACACT GTCACAGTGA CTCCTGTTTA TTCTACTGGA 
GAGGGGAAGC CCATGTCTGA AAACGGCAAA ACATTGGAGC GCAGTCCTGC TAGGAATATC 
CAGGTTTTCA ACCCCACAAC TAACACTTTG AATGTCCGCT GGGAAGCAGC CACCGGGCCG 
GTCCAGCAGT ACAGGGTGGT CTATGCTCCT TTGACTGGTG TGAGGCCAAC GGAGTCGATT 
TTGGTCCCCG GCACCACAAC CACAGCACTG CTGCAGCAGC TGGTCCCAGA CACAGCCTAC 
AATGTGGGAG TTGTTGCCTT GTACAGCGAT GGGGAAGGAA CCACTGTCAG CGATCAGGGA 
AAGACTTTGC CCCGGGGTGG TCCCAGAAAC ATGCGTGTGT ACGATCCCAC CACCAGTACG 
CTGTCTGTTA ACTGGGAACA CGCTGACGGT CCTGTTCTTC AGTACCGCAT CAGCTATGCC 
CCAACAACAG GAGACCCTAT CGAGGAATTT ACCACAGTTC AAGGGAACAG GAACAATGTG 
GTTCTTCAGA ATCTGGATCC AGACACTCCC TACAATATCA GAGTGACTGC TATGTATGCT 
GAAGGAGCGG GAGGAGAACT GGAGGGAGAT GGACGTACAG TTGGAATGCT CGGCCCCAGA 
AACCTGCGTG TATCTGATGA ATGGTACACT CGCTTCAGGG TGTCCTGGGA CCCCGCCCCC 
TCCGCAGTCA CTGGATACAA ACTCATCTAC CAGCCTGCGG GGTCTTATGA CTCATCTGAG 
GTGTTTGTTG GAGATGTGAC CACCTATCAG CTGCAAAACT TGAAGCCTGG AACTACCTAT 
GACTTGACGG TCCTAGCACA GTACAGAACG GGCATCAGTG GTCCTCTAAA TGGTCAAGGC 
ACCACATTGT ACCTCAATGT GACAGGCCTA ACCTCCTACA ATGTTGGCTA TGACTCCTTT 
TGCATCCAGT GGGTCCCTCA CCGAGCAGCC ACGTCGTACA GACTTAAAGT CAACCCTTTT 
GAGTCCTTCA AGGGTGGAGC TCAAGAAATC ACAGTTCAGG GCCCAGAGAG TAAATACTGC 
TTTGACAACC TGACCCCTGA CACTCTTTAC AACGCTACGG TTTACGCTCA GACCCTCAAC 
CTTGAAGGAC CTGGAGTCAG TATTAAGGAG AAAACATTGG TCAAACCCAC CGAGGTGCCA 
ACCGAGCCTC CCACCCCTCC CCCTCCATCA ATAGTGCCTG ATGCTCTTGA CATGTGTAAA 
GGTGCGAAGG CTGACTTGGT CTTCCTCATC GACGGCTCAT GGAGTATTGG AGATGACAGC 
TTTGCCAAAG TCCTCCAGTT TGTCACTAGT GTGACAGCAG CTTTTGATGT TGTTAGCCCA 
ACTGGAATGC AGGTTTCATT TGTGCAGTAT AGCGATGATG CTAAGACTGA GTTCAAACTG 
AACACCTACC ACAACAAGGG CATTGTCCTA TCTGCACTGA AGTCAATCCG CTACAGGGGA 
GGCAACACTA AGACTGGCAT TGCTCTGAAG CACGTCTATG AAAAGGTACT AACGTCCGAC 
AATGGCATGA GGAGGAATGT CCCGAAGGTG CTGGTTGTTG TCACAGATGG TCGTTCTCAA 
GATGAAGTCA AGAAGAGCGC AGAAAAACTT CAGCATTCTG GCTATAGTGT GTTTGTGGTA 
GGAGTGGCTG ATGTGAGCAT GAGAGAACTG GCAGTTATCG GCAGCAAGCC CACCGAAAGA 
CACGTGTTTG TCGTTGACGA TTACGACGCT TTTTCCAAAA TCCAGGACAA CTTGATCACA 
TTCATCTGTG AAACGGCTAC CTCCACTTGC CCTCTCATCT ACATCAATGG ATACACCACA 
CCTGGATTTA GGATGCTGGA AGCTTTCAAC ATCACAGACC GAACATTTGC TGGCATGAAT 
GGTGTCTCCA TGGAGCCAGG TTCTTTCAAC AGCTTCATTG CCTACAGGCT GCACAAAGAT 
GCCTTCCTAA ACCAGCCCAC AAAGGAAATC CACTCCGACG GCCTTCCTCC ATCCTACACT 
ATAATCCTGC TCCTCCGCCT GCTGCCTGAT ACACCCTCTG AACCTTTTGA TATCTGGCAG 
ATCTCTGACA AAAACAACAA CCCTGAAGTT GGAGTCACTA TGAATCCTTC CAGCAAAACA 
CTCACGTTCT ACAACAAGGA CACCCGGGGA GAGATCCAGA GAGCCGTGTT CAATGAGGAG 
CAAGTGAAAC GGGTTTTCCA CGGAAGCTTC CACAAGCTCC ACATTACCGT CTCCCCAGAG 
AAGGTCAAGC TCAACGTGGA CTGCCAAGAG GTGGCAGAGA AGCCAATCAA GGAGGCCAAC 
AACATCACGC TGGAGGGCTA TGAAGTGCTT GGCAAGATGG TCAAATCAGG AGGTGGAAAG 
AGGCAGTCCG CTACATTCCA GCTCCAGATG TTTGACATTG TCTGCAGCTT GAGTTGGATC 
AGCCGTGACA AATGCTGTGA CCTGCCTGCT ACGAGAATTG AGTCCAAATG TCCTGCTCTT 
CCCCACTCCT GCACATGCAC CCAAGACAGT GTTGGGCCCC AGGGGCCTCC TGGGCCTTCG 
GGCAGCCCAG GGTCTAAAGG ACCCAGAGGA GACAGGGGAG ACCCAGGCAA ACCTGGTGCA 
ATGGGTCCAC GAGGTGAGCC AGGACTCCAA GGTGCAATGG GCCCACCAGG CCCACAAGGC 
CCTAATGGAC TGTCTATCCC CGGAGAACCG GGTCGCCAAG GACCAAAGGG AGATCGTGGA 
GACTCAGGCC TGCCTGGGCT GCAAGGTCCT CCTGGGCCGC GTGGTCCTAT GGGTCCTGTC 
GGACAAAGTG GAGCACGGGG GCCACCAGGA AAGGAAGGAT CATCTGGACC CAGAGGCCCA 
CCAGGGCCCA TGGGAAGTCC AGGAAATCCA GGTGTACCTG GTATGTCTGG AAAACCAGGA 
AAGTCAGGAG AACCAGGAAG TCCAGGACCT GTTGGCCTTA AAGGAGAAAA GGGAGAGCGG 
GGAGACTTTG CATCCCAAAA TATGATGCGC TCCATTGCCA GACAAGTTTG TGAACAGCTC 
GTGAGCAGTC AGATGAGCAG AATTGACGGG ATGCTCAATC AAATTCCTTC TGGATATCGT 
AATGGCAATC CAGGCCCGGC TGGTCCTCCT GGTCCCCCTG GCAACCAAGG ACCTCGTGGA 
GAGCCCGGAC AACCTGGAAA ATCTGGTTTC CCTGGAAGCC CAGGATTACC TGGAAATCAA 
GGAGAAAGAG GGTTACCAGG AGAGAGGGGG GAGAGAGGAC CTCCAGGAGC AGATATCAGA 
GGACAAAGAG GTCCACCTGG ACCAGCAGGT CCACCAGGGG AGTCTAGAAC AGGTCCCCCA 
GGTTCTCCTG GCTCTGCTGG ATCCCGTGGC CCCCCAGGTC GTCAAGGTAA TCCAGGTGTG 
AGAGGACCAC CAGGACCCCC TGGATACTGT GACTCCTCTC AGTGTGTTGG TATTCCTTAC 
AATGGACAAG GATACACAGG TTTGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488806.1
CDS	230..9439
Translation	MKIRLSLATAAFLVGFLASIDAQVEPPSDLKFKILNENTVEMSWLKPSSRIEGFRIQVASDADEPSRDFTLDAYSTSTSITNLTPDVDYSVSISSYYGSEESISIFGQLTIQSSNTTRPRPTSDALKCSVSAIADLVFLVDGSWSVGRENFKHIRSFIGSLAGAFDIGEDKTRVAVVQYSTDTRTEFPLNRYTRRGDLLQAINSLPYKGGNTMTGDAIDYLIKNIFTEAAGSRKAFPKVAMIITDGKSQDPVEEHARRLRNIGVEIFVLGIKGADEDELREIASTPHAKHVYNVPNFDLIQEVQKRIITEVCSGVDDQLTSLVSGEEMVEAPSNLQVTEIASKSMRLTWDASLGDITGYKLTLIPLVPGMKRQELYMGATQTSINVRDLSPETEYDISLFALKGLTPSEPLTDLAKTQPVKVLTECSMDVDVQADVVLLVDGSYSIGLQNFAKVREFLEVLVKSFDIGPDKIQISLVQYSRDPHTEFYLNTHHDINAVVKAVRNFPYRGGSTNTGKAMTYVREKIFVPTRGARQNVPRVMVLITDGKSSDSFRVAATKLRNIDVEIFAVGVKDAVRSELEAIANEPADTHVYEVEDFDAFQRISKELTQSICLRIEQELLKIKKKNLLPPSNLQFSEITSRSFRATWVAPAASVMSYLVRYRKAEDITGDYISLAVPGQTTTTVLLYLTPITTYEVNVYAEYDKGDSFALTGQETTLEEQGTVENLRVTEETTNSFRVSWQAAPGSVIRYRLSYVPLSGMGEILEAQTLGPETTIVLQELFPITTYRVSVSPEYAAGVGDELQVDGTTKEVIGSPRDLRVFDETINTMKVAWQAAPGNVVQYHVTYKLAEGGDINMELVQGDITQALLKNLQPATEYELFVTAHYSTGQGDPLMGAGTTLEELGSPRDLETRDVTDTSFAVSWTAAPGNVRQYRIKWKSMFSEETGEKTVAGDTTTADLDGLTPETRYQVSVFARYGHGEGQPLVGEETTDISANGRVIVVSDETEKSMKVTWQPAPGNVLNYRVTYKPQSGGRQLAAKVPGGNTSTVLRRLTSLTTYDITVVPVYRFGEGKIRQGEGTTLTPYKGPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVSFHPAEDDIDLRELLVSPHDNTVVLEELRAGTKYTVSVFGMFDGGESMPLAGEENTTYSDDPDSSPVDVPKMSCKTKAQADIVLLVDGSWSIGRLNFKTIRSFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHQTRESLLEAVAKLPYKGGNTMTGLALYYILQNNFQESVGMRPGARKIGVLITDGKSQDEIIASSQSLRDAGIELYAIGVKNADENELRAIASDPDEIHMYNVADFSFLLDIVDTLSDNLCNSVTGSDNTPGSPTNLVTSEVTHQSFRATWTGPEGPVEKYRVEYMTVSGAPQEVLVDGTVTTVVLRGLDPLTKYLVNVYSVIGEESSEPLKGTETTLPLSAVREMSVYDEQMTTMRARWKAVQGATGYLLTYRATNATQPTVEQEMRVAAGTTDVQLVKLTPNTAYSLSLYALYGLSASEALLRQGVTLPMPPAGELNVRDVTHSTMVLYWDAAPGAVRKYIITYTPEDGETKELEVGGRITTKLLENLISQTEYSLTVTPVYDEGRGQAMLGSGITDVVPAPKNLRFSEITQTSFRATWEHGAPDVAMYRIAWRRKGETINEGEEILSEEETTFVLENLDPDTPYDVSVTAIYPDESESEDLLGTERTLSKSDGRPIPNAPPTNLVVFNETTTTLNARWTPPLGRVQNYRITYVPTSGGQSQTTMVGAKKNTVLLPKLTPDTEYSIGVVAVYANGVSRELNGLGKTKPLGGVRNLRVTNPTTSTLDVNWEPAEGNVRQYRVFYVPAAGGEQEMIQVSGSTYKAVLKNLQSDTVYTVTVTPVYSTGEGKPMSENGKTLERSPARNIQVFNPTTNTLNVRWEAATGPVQQYRVVYAPLTGVRPTESILVPGTTTTALLQQLVPDTAYNVGVVALYSDGEGTTVSDQGKTLPRGGPRNMRVYDPTTSTLSVNWEHADGPVLQYRISYAPTTGDPIEEFTTVQGNRNNVVLQNLDPDTPYNIRVTAMYAEGAGGELEGDGRTVGMLGPRNLRVSDEWYTRFRVSWDPAPSAVTGYKLIYQPAGSYDSSEVFVGDVTTYQLQNLKPGTTYDLTVLAQYRTGISGPLNGQGTTLYLNVTGLTSYNVGYDSFCIQWVPHRAATSYRLKVNPFESFKGGAQEITVQGPESKYCFDNLTPDTLYNATVYAQTLNLEGPGVSIKEKTLVKPTEVPTEPPTPPPPSIVPDALDMCKGAKADLVFLIDGSWSIGDDSFAKVLQFVTSVTAAFDVVSPTGMQVSFVQYSDDAKTEFKLNTYHNKGIVLSALKSIRYRGGNTKTGIALKHVYEKVLTSDNGMRRNVPKVLVVVTDGRSQDEVKKSAEKLQHSGYSVFVVGVADVSMRELAVIGSKPTERHVFVVDDYDAFSKIQDNLITFICETATSTCPLIYINGYTTPGFRMLEAFNITDRTFAGMNGVSMEPGSFNSFIAYRLHKDAFLNQPTKEIHSDGLPPSYTIILLLRLLPDTPSEPFDIWQISDKNNNPEVGVTMNPSSKTLTFYNKDTRGEIQRAVFNEEQVKRVFHGSFHKLHITVSPEKVKLNVDCQEVAEKPIKEANNITLEGYEVLGKMVKSGGGKRQSATFQLQMFDIVCSLSWISRDKCCDLPATRIESKCPALPHSCTCTQDSVGPQGPPGPSGSPGSKGPRGDRGDPGKPGAMGPRGEPGLQGAMGPPGPQGPNGLSIPGEPGRQGPKGDRGDSGLPGLQGPPGPRGPMGPVGQSGARGPPGKEGSSGPRGPPGPMGSPGNPGVPGMSGKPGKSGEPGSPGPVGLKGEKGERGDFASQNMMRSIARQVCEQLVSSQMSRIDGMLNQIPSGYRNGNPGPAGPPGPPGNQGPRGEPGQPGKSGFPGSPGLPGNQGERGLPGERGERGPPGADIRGQRGPPGPAGPPGESRTGPPGSPGSAGSRGPPGRQGNPGVRGPPGPPGYCDSSQCVGIPYNGQGYTGLA

Target ORF information:

RefSeq Version	XM_011490504.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490504.3

ORF Insert Sequence:

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TGTCCAAGTC AGATGGTAGA CCGATACCAA ATGCACCTCC CACCAATTTG 
GTTGTTTTCA ATGAGACCAC CACCACCCTG AATGCTAGGT GGACTCCACC TCTGGGTCGT 
GTCCAGAACT ACAGGATCAC TTATGTCCCC ACATCTGGAG GCCAGAGTCA GACTACCATG 
GTTGGAGCCA AGAAAAACAC AGTCCTGCTT CCAAAGCTGA CCCCTGACAC AGAGTACTCC 
ATCGGTGTTG TGGCGGTGTA CGCCAATGGA GTCAGCAGGG AGCTGAATGG CCTTGGAAAA 
ACCAAACCTT TGGGTGGTGT TAGAAACCTG CGTGTGACAA ACCCCACCAC GAGCACGTTG 
GATGTGAACT GGGAACCTGC TGAGGGTAAT GTGCGCCAGT ATAGGGTCTT CTATGTTCCT 
GCAGCTGGAG GAGAGCAGGA GATGATTCAG GTCTCCGGAA GCACCTACAA AGCAGTTCTG 
AAGAACCTGC AGTCGGACAC TGTCTACACT GTCACAGTGA CTCCTGTTTA TTCTACTGGA 
GAGGGGAAGC CCATGTCTGA AAACGGCAAA ACATTGGAGC GCAGTCCTGC TAGGAATATC 
CAGGTTTTCA ACCCCACAAC TAACACTTTG AATGTCCGCT GGGAAGCAGC CACCGGGCCG 
GTCCAGCAGT ACAGGGTGGT CTATGCTCCT TTGACTGGTG TGAGGCCAAC GGAGTCGATT 
TTGGTCCCCG GCACCACAAC CACAGCACTG CTGCAGCAGC TGGTCCCAGA CACAGCCTAC 
AATGTGGGAG TTGTTGCCTT GTACAGCGAT GGGGAAGGAA CCACTGTCAG CGATCAGGGA 
AAGACTTTGC CCCGGGGTGG TCCCAGAAAC ATGCGTGTGT ACGATCCCAC CACCAGTACG 
CTGTCTGTTA ACTGGGAACA CGCTGACGGT CCTGTTCTTC AGTACCGCAT CAGCTATGCC 
CCAACAACAG GAGACCCTAT CGAGGAATTT ACCACAGTTC AAGGGAACAG GAACAATGTG 
GTTCTTCAGA ATCTGGATCC AGACACTCCC TACAATATCA GAGTGACTGC TATGTATGCT 
GAAGGAGCGG GAGGAGAACT GGAGGGAGAT GGACGTACAG TTGGAATGCT CGGCCCCAGA 
AACCTGCGTG TATCTGATGA ATGGTACACT CGCTTCAGGG TGTCCTGGGA CCCCGCCCCC 
TCCGCAGTCA CTGGATACAA ACTCATCTAC CAGCCTGCGG GGTCTTATGA CTCATCTGAG 
GTGTTTGTTG GAGATGTGAC CACCTATCAG CTGCAAAACT TGAAGCCTGG AACTACCTAT 
GACTTGACGG TCCTAGCACA GTACAGAACG GGCATCAGTG GTCCTCTAAA TGGTCAAGGC 
ACCACATTGT ACCTCAATGT GACAGGCCTA ACCTCCTACA ATGTTGGCTA TGACTCCTTT 
TGCATCCAGT GGGTCCCTCA CCGAGCAGCC ACGTCGTACA GACTTAAAGT CAACCCTTTT 
GAGTCCTTCA AGGGTGGAGC TCAAGAAATC ACAGTTCAGG GCCCAGAGAG TAAATACTGC 
TTTGACAACC TGACCCCTGA CACTCTTTAC AACGCTACGG TTTACGCTCA GACCCTCAAC 
CTTGAAGGAC CTGGAGTCAG TATTAAGGAG AAAACATTGG TCAAACCCAC CGAGGTGCCA 
ACCGAGCCTC CCACCCCTCC CCCTCCATCA ATAGTGCCTG ATGCTCTTGA CATGTGTAAA 
GGTGCGAAGG CTGACTTGGT CTTCCTCATC GACGGCTCAT GGAGTATTGG AGATGACAGC 
TTTGCCAAAG TCCTCCAGTT TGTCACTAGT GTGACAGCAG CTTTTGATGT TGTTAGCCCA 
ACTGGAATGC AGGTTTCATT TGTGCAGTAT AGCGATGATG CTAAGACTGA GTTCAAACTG 
AACACCTACC ACAACAAGGG CATTGTCCTA TCTGCACTGA AGTCAATCCG CTACAGGGGA 
GGCAACACTA AGACTGGCAT TGCTCTGAAG CACGTCTATG AAAAGGTACT AACGTCCGAC 
AATGGCATGA GGAGGAATGT CCCGAAGGTG CTGGTTGTTG TCACAGATGG TCGTTCTCAA 
GATGAAGTCA AGAAGAGCGC AGAAAAACTT CAGCATTCTG GCTATAGTGT GTTTGTGGTA 
GGAGTGGCTG ATGTGAGCAT GAGAGAACTG GCAGTTATCG GCAGCAAGCC CACCGAAAGA 
CACGTGTTTG TCGTTGACGA TTACGACGCT TTTTCCAAAA TCCAGGACAA CTTGATCACA 
TTCATCTGTG AAACGGCTAC CTCCACTTGC CCTCTCATCT ACATCAATGG ATACACCACA 
CCTGGATTTA GGATGCTGGA AGCTTTCAAC ATCACAGACC GAACATTTGC TGGCATGAAT 
GGTGTCTCCA TGGAGCCAGG TTCTTTCAAC AGCTTCATTG CCTACAGGCT GCACAAAGAT 
GCCTTCCTAA ACCAGCCCAC AAAGGAAATC CACTCCGACG GCCTTCCTCC ATCCTACACT 
ATAATCCTGC TCCTCCGCCT GCTGCCTGAT ACACCCTCTG AACCTTTTGA TATCTGGCAG 
ATCTCTGACA AAAACAACAA CCCTGAAGTT GGAGTCACTA TGAATCCTTC CAGCAAAACA 
CTCACGTTCT ACAACAAGGA CACCCGGGGA GAGATCCAGA GAGCCGTGTT CAATGAGGAG 
CAAGTGAAAC GGGTTTTCCA CGGAAGCTTC CACAAGCTCC ACATTACCGT CTCCCCAGAG 
AAGGTCAAGC TCAACGTGGA CTGCCAAGAG GTGGCAGAGA AGCCAATCAA GGAGGCCAAC 
AACATCACGC TGGAGGGCTA TGAAGTGCTT GGCAAGATGG TCAAATCAGG AGGTGGAAAG 
AGGCAGTCCG CTACATTCCA GCTCCAGATG TTTGACATTG TCTGCAGCTT GAGTTGGATC 
AGCCGTGACA AATGCTGTGA CCTGCCTGCT ACGAGAATTG AGTCCAAATG TCCTGCTCTT 
CCCCACTCCT GCACATGCAC CCAAGACAGT GTTGGGCCCC AGGGGCCTCC TGGGCCTTCG 
GGCAGCCCAG GGTCTAAAGG ACCCAGAGGA GACAGGGGAG ACCCAGGCAA ACCTGGTGCA 
ATGGGTCCAC GAGGTGAGCC AGGACTCCAA GGTGCAATGG GCCCACCAGG CCCACAAGGC 
CCTAATGGAC TGTCTATCCC CGGAGAACCG GGTCGCCAAG GACCAAAGGG AGATCGTGGA 
GACTCAGGCC TGCCTGGGCT GCAAGGTCCT CCTGGGCCGC GTGGTCCTAT GGGTCCTGTC 
GGACAAAGTG GAGCACGGGG GCCACCAGGA AAGGAAGGAT CATCTGGACC CAGAGGCCCA 
CCAGGGCCCA TGGGAAGTCC AGGAAATCCA GGTGTACCTG GTATGTCTGG AAAACCAGGA 
AAGTCAGGAG AACCAGGAAG TCCAGGACCT GTTGGCCTTA AAGGAGAAAA GGGAGAGCGG 
GGAGACTTTG CATCCCAAAA TATGATGCGC TCCATTGCCA GACAAGTTTG TGAACAGCTC 
GTGAGCAGTC AGATGAGCAG AATTGACGGG ATGCTCAATC AAATTCCTTC TGGATATCGT 
AATGGCAATC CAGGCCCGGC TGGTCCTCCT GGTCCCCCTG GCAACCAAGG ACCTCGTGGA 
GAGCCCGGAC AACCTGGAAA ATCTGGTTTC CCTGGAAGCC CAGGATTACC TGGAAATCAA 
GGAGAAAGAG GGTTACCAGG AGAGAGGGGG GAGAGAGGAC CTCCAGGAGC AGATATCAGA 
GGACAAAGAG GTCCACCTGG ACCAGCAGGT CCACCAGGGG AGTCTAGAAC AGGTCCCCCA 
GGTTCTCCTG GCTCTGCTGG ATCCCGTGGC CCCCCAGGTC GTCAAGGTAA TCCAGGTGTG 
AGAGGACCAC CAGGACCCCC TGGATACTGT GACTCCTCTC AGTGTGTTGG TATTCCTTAC 
AATGGACAAG GATACACAGG TTTGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col12a1 ( XM_011490504.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488806.1 Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X3, mRNA.

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CloneID	OJa24263
Clone ID Related Accession (Same CDS sequence)	XM_011490504.3 , XM_011490504.2
Accession Version	XM_011490504.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	collagen alpha-1(XII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011490504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TGTCCAAGTC AGATGGTAGA CCGATACCAA ATGCACCTCC CACCAATTTG 
GTTGTTTTCA ATGAGACCAC CACCACCCTG AATGCTAGGT GGACTCCACC TCTGGGTCGT 
GTCCAGAACT ACAGGATCAC TTATGTCCCC ACATCTGGAG GCCAGAGTCA GACTACCATG 
GTTGGAGCCA AGAAAAACAC AGTCCTGCTT CCAAAGCTGA CCCCTGACAC AGAGTACTCC 
ATCGGTGTTG TGGCGGTGTA CGCCAATGGA GTCAGCAGGG AGCTGAATGG CCTTGGAAAA 
ACCAAACCTT TGGGTGGTGT TAGAAACCTG CGTGTGACAA ACCCCACCAC GAGCACGTTG 
GATGTGAACT GGGAACCTGC TGAGGGTAAT GTGCGCCAGT ATAGGGTCTT CTATGTTCCT 
GCAGCTGGAG GAGAGCAGGA GATGATTCAG GTCTCCGGAA GCACCTACAA AGCAGTTCTG 
AAGAACCTGC AGTCGGACAC TGTCTACACT GTCACAGTGA CTCCTGTTTA TTCTACTGGA 
GAGGGGAAGC CCATGTCTGA AAACGGCAAA ACATTGGAGC GCAGTCCTGC TAGGAATATC 
CAGGTTTTCA ACCCCACAAC TAACACTTTG AATGTCCGCT GGGAAGCAGC CACCGGGCCG 
GTCCAGCAGT ACAGGGTGGT CTATGCTCCT TTGACTGGTG TGAGGCCAAC GGAGTCGATT 
TTGGTCCCCG GCACCACAAC CACAGCACTG CTGCAGCAGC TGGTCCCAGA CACAGCCTAC 
AATGTGGGAG TTGTTGCCTT GTACAGCGAT GGGGAAGGAA CCACTGTCAG CGATCAGGGA 
AAGACTTTGC CCCGGGGTGG TCCCAGAAAC ATGCGTGTGT ACGATCCCAC CACCAGTACG 
CTGTCTGTTA ACTGGGAACA CGCTGACGGT CCTGTTCTTC AGTACCGCAT CAGCTATGCC 
CCAACAACAG GAGACCCTAT CGAGGAATTT ACCACAGTTC AAGGGAACAG GAACAATGTG 
GTTCTTCAGA ATCTGGATCC AGACACTCCC TACAATATCA GAGTGACTGC TATGTATGCT 
GAAGGAGCGG GAGGAGAACT GGAGGGAGAT GGACGTACAG TTGGAATGCT CGGCCCCAGA 
AACCTGCGTG TATCTGATGA ATGGTACACT CGCTTCAGGG TGTCCTGGGA CCCCGCCCCC 
TCCGCAGTCA CTGGATACAA ACTCATCTAC CAGCCTGCGG GGTCTTATGA CTCATCTGAG 
GTGTTTGTTG GAGATGTGAC CACCTATCAG CTGCAAAACT TGAAGCCTGG AACTACCTAT 
GACTTGACGG TCCTAGCACA GTACAGAACG GGCATCAGTG GTCCTCTAAA TGGTCAAGGC 
ACCACATTGT ACCTCAATGT GACAGGCCTA ACCTCCTACA ATGTTGGCTA TGACTCCTTT 
TGCATCCAGT GGGTCCCTCA CCGAGCAGCC ACGTCGTACA GACTTAAAGT CAACCCTTTT 
GAGTCCTTCA AGGGTGGAGC TCAAGAAATC ACAGTTCAGG GCCCAGAGAG TAAATACTGC 
TTTGACAACC TGACCCCTGA CACTCTTTAC AACGCTACGG TTTACGCTCA GACCCTCAAC 
CTTGAAGGAC CTGGAGTCAG TATTAAGGAG AAAACATTGG TCAAACCCAC CGAGGTGCCA 
ACCGAGCCTC CCACCCCTCC CCCTCCATCA ATAGTGCCTG ATGCTCTTGA CATGTGTAAA 
GGTGCGAAGG CTGACTTGGT CTTCCTCATC GACGGCTCAT GGAGTATTGG AGATGACAGC 
TTTGCCAAAG TCCTCCAGTT TGTCACTAGT GTGACAGCAG CTTTTGATGT TGTTAGCCCA 
ACTGGAATGC AGGTTTCATT TGTGCAGTAT AGCGATGATG CTAAGACTGA GTTCAAACTG 
AACACCTACC ACAACAAGGG CATTGTCCTA TCTGCACTGA AGTCAATCCG CTACAGGGGA 
GGCAACACTA AGACTGGCAT TGCTCTGAAG CACGTCTATG AAAAGGTACT AACGTCCGAC 
AATGGCATGA GGAGGAATGT CCCGAAGGTG CTGGTTGTTG TCACAGATGG TCGTTCTCAA 
GATGAAGTCA AGAAGAGCGC AGAAAAACTT CAGCATTCTG GCTATAGTGT GTTTGTGGTA 
GGAGTGGCTG ATGTGAGCAT GAGAGAACTG GCAGTTATCG GCAGCAAGCC CACCGAAAGA 
CACGTGTTTG TCGTTGACGA TTACGACGCT TTTTCCAAAA TCCAGGACAA CTTGATCACA 
TTCATCTGTG AAACGGCTAC CTCCACTTGC CCTCTCATCT ACATCAATGG ATACACCACA 
CCTGGATTTA GGATGCTGGA AGCTTTCAAC ATCACAGACC GAACATTTGC TGGCATGAAT 
GGTGTCTCCA TGGAGCCAGG TTCTTTCAAC AGCTTCATTG CCTACAGGCT GCACAAAGAT 
GCCTTCCTAA ACCAGCCCAC AAAGGAAATC CACTCCGACG GCCTTCCTCC ATCCTACACT 
ATAATCCTGC TCCTCCGCCT GCTGCCTGAT ACACCCTCTG AACCTTTTGA TATCTGGCAG 
ATCTCTGACA AAAACAACAA CCCTGAAGTT GGAGTCACTA TGAATCCTTC CAGCAAAACA 
CTCACGTTCT ACAACAAGGA CACCCGGGGA GAGATCCAGA GAGCCGTGTT CAATGAGGAG 
CAAGTGAAAC GGGTTTTCCA CGGAAGCTTC CACAAGCTCC ACATTACCGT CTCCCCAGAG 
AAGGTCAAGC TCAACGTGGA CTGCCAAGAG GTGGCAGAGA AGCCAATCAA GGAGGCCAAC 
AACATCACGC TGGAGGGCTA TGAAGTGCTT GGCAAGATGG TCAAATCAGG AGGTGGAAAG 
AGGCAGTCCG CTACATTCCA GCTCCAGATG TTTGACATTG TCTGCAGCTT GAGTTGGATC 
AGCCGTGACA AATGCTGTGA CCTGCCTGCT ACGAGAATTG AGTCCAAATG TCCTGCTCTT 
CCCCACTCCT GCACATGCAC CCAAGACAGT GTTGGGCCCC AGGGGCCTCC TGGGCCTTCG 
GGCAGCCCAG GGTCTAAAGG ACCCAGAGGA GACAGGGGAG ACCCAGGCAA ACCTGGTGCA 
ATGGGTCCAC GAGGTGAGCC AGGACTCCAA GGTGCAATGG GCCCACCAGG CCCACAAGGC 
CCTAATGGAC TGTCTATCCC CGGAGAACCG GGTCGCCAAG GACCAAAGGG AGATCGTGGA 
GACTCAGGCC TGCCTGGGCT GCAAGGTCCT CCTGGGCCGC GTGGTCCTAT GGGTCCTGTC 
GGACAAAGTG GAGCACGGGG GCCACCAGGA AAGGAAGGAT CATCTGGACC CAGAGGCCCA 
CCAGGGCCCA TGGGAAGTCC AGGAAATCCA GGTGTACCTG GTATGTCTGG AAAACCAGGA 
AAGTCAGGAG AACCAGGAAG TCCAGGACCT GTTGGCCTTA AAGGAGAAAA GGGAGAGCGG 
GGAGACTTTG CATCCCAAAA TATGATGCGC TCCATTGCCA GACAAGTTTG TGAACAGCTC 
GTGAGCAGTC AGATGAGCAG AATTGACGGG ATGCTCAATC AAATTCCTTC TGGATATCGT 
AATGGCAATC CAGGCCCGGC TGGTCCTCCT GGTCCCCCTG GCAACCAAGG ACCTCGTGGA 
GAGCCCGGAC AACCTGGAAA ATCTGGTTTC CCTGGAAGCC CAGGATTACC TGGAAATCAA 
GGAGAAAGAG GGTTACCAGG AGAGAGGGGG GAGAGAGGAC CTCCAGGAGC AGATATCAGA 
GGACAAAGAG GTCCACCTGG ACCAGCAGGT CCACCAGGGG AGTCTAGAAC AGGTCCCCCA 
GGTTCTCCTG GCTCTGCTGG ATCCCGTGGC CCCCCAGGTC GTCAAGGTAA TCCAGGTGTG 
AGAGGACCAC CAGGACCCCC TGGATACTGT GACTCCTCTC AGTGTGTTGG TATTCCTTAC 
AATGGACAAG GATACACAGG TTTGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488806.1
CDS	231..9440
Translation	MKIRLSLATAAFLVGFLASIDAQVEPPSDLKFKILNENTVEMSWLKPSSRIEGFRIQVASDADEPSRDFTLDAYSTSTSITNLTPDVDYSVSISSYYGSEESISIFGQLTIQSSNTTRPRPTSDALKCSVSAIADLVFLVDGSWSVGRENFKHIRSFIGSLAGAFDIGEDKTRVAVVQYSTDTRTEFPLNRYTRRGDLLQAINSLPYKGGNTMTGDAIDYLIKNIFTEAAGSRKAFPKVAMIITDGKSQDPVEEHARRLRNIGVEIFVLGIKGADEDELREIASTPHAKHVYNVPNFDLIQEVQKRIITEVCSGVDDQLTSLVSGEEMVEAPSNLQVTEIASKSMRLTWDASLGDITGYKLTLIPLVPGMKRQELYMGATQTSINVRDLSPETEYDISLFALKGLTPSEPLTDLAKTQPVKVLTECSMDVDVQADVVLLVDGSYSIGLQNFAKVREFLEVLVKSFDIGPDKIQISLVQYSRDPHTEFYLNTHHDINAVVKAVRNFPYRGGSTNTGKAMTYVREKIFVPTRGARQNVPRVMVLITDGKSSDSFRVAATKLRNIDVEIFAVGVKDAVRSELEAIANEPADTHVYEVEDFDAFQRISKELTQSICLRIEQELLKIKKKNLLPPSNLQFSEITSRSFRATWVAPAASVMSYLVRYRKAEDITGDYISLAVPGQTTTTVLLYLTPITTYEVNVYAEYDKGDSFALTGQETTLEEQGTVENLRVTEETTNSFRVSWQAAPGSVIRYRLSYVPLSGMGEILEAQTLGPETTIVLQELFPITTYRVSVSPEYAAGVGDELQVDGTTKEVIGSPRDLRVFDETINTMKVAWQAAPGNVVQYHVTYKLAEGGDINMELVQGDITQALLKNLQPATEYELFVTAHYSTGQGDPLMGAGTTLEELGSPRDLETRDVTDTSFAVSWTAAPGNVRQYRIKWKSMFSEETGEKTVAGDTTTADLDGLTPETRYQVSVFARYGHGEGQPLVGEETTDISANGRVIVVSDETEKSMKVTWQPAPGNVLNYRVTYKPQSGGRQLAAKVPGGNTSTVLRRLTSLTTYDITVVPVYRFGEGKIRQGEGTTLTPYKGPRNLQTSEPTKTSFRVTWDHAPGDVKGYKVSFHPAEDDIDLRELLVSPHDNTVVLEELRAGTKYTVSVFGMFDGGESMPLAGEENTTYSDDPDSSPVDVPKMSCKTKAQADIVLLVDGSWSIGRLNFKTIRSFIARMVGVFDIGPDRVQIGLAQYSGDPKTEWHLNAHQTRESLLEAVAKLPYKGGNTMTGLALYYILQNNFQESVGMRPGARKIGVLITDGKSQDEIIASSQSLRDAGIELYAIGVKNADENELRAIASDPDEIHMYNVADFSFLLDIVDTLSDNLCNSVTGSDNTPGSPTNLVTSEVTHQSFRATWTGPEGPVEKYRVEYMTVSGAPQEVLVDGTVTTVVLRGLDPLTKYLVNVYSVIGEESSEPLKGTETTLPLSAVREMSVYDEQMTTMRARWKAVQGATGYLLTYRATNATQPTVEQEMRVAAGTTDVQLVKLTPNTAYSLSLYALYGLSASEALLRQGVTLPMPPAGELNVRDVTHSTMVLYWDAAPGAVRKYIITYTPEDGETKELEVGGRITTKLLENLISQTEYSLTVTPVYDEGRGQAMLGSGITDVVPAPKNLRFSEITQTSFRATWEHGAPDVAMYRIAWRRKGETINEGEEILSEEETTFVLENLDPDTPYDVSVTAIYPDESESEDLLGTERTLSKSDGRPIPNAPPTNLVVFNETTTTLNARWTPPLGRVQNYRITYVPTSGGQSQTTMVGAKKNTVLLPKLTPDTEYSIGVVAVYANGVSRELNGLGKTKPLGGVRNLRVTNPTTSTLDVNWEPAEGNVRQYRVFYVPAAGGEQEMIQVSGSTYKAVLKNLQSDTVYTVTVTPVYSTGEGKPMSENGKTLERSPARNIQVFNPTTNTLNVRWEAATGPVQQYRVVYAPLTGVRPTESILVPGTTTTALLQQLVPDTAYNVGVVALYSDGEGTTVSDQGKTLPRGGPRNMRVYDPTTSTLSVNWEHADGPVLQYRISYAPTTGDPIEEFTTVQGNRNNVVLQNLDPDTPYNIRVTAMYAEGAGGELEGDGRTVGMLGPRNLRVSDEWYTRFRVSWDPAPSAVTGYKLIYQPAGSYDSSEVFVGDVTTYQLQNLKPGTTYDLTVLAQYRTGISGPLNGQGTTLYLNVTGLTSYNVGYDSFCIQWVPHRAATSYRLKVNPFESFKGGAQEITVQGPESKYCFDNLTPDTLYNATVYAQTLNLEGPGVSIKEKTLVKPTEVPTEPPTPPPPSIVPDALDMCKGAKADLVFLIDGSWSIGDDSFAKVLQFVTSVTAAFDVVSPTGMQVSFVQYSDDAKTEFKLNTYHNKGIVLSALKSIRYRGGNTKTGIALKHVYEKVLTSDNGMRRNVPKVLVVVTDGRSQDEVKKSAEKLQHSGYSVFVVGVADVSMRELAVIGSKPTERHVFVVDDYDAFSKIQDNLITFICETATSTCPLIYINGYTTPGFRMLEAFNITDRTFAGMNGVSMEPGSFNSFIAYRLHKDAFLNQPTKEIHSDGLPPSYTIILLLRLLPDTPSEPFDIWQISDKNNNPEVGVTMNPSSKTLTFYNKDTRGEIQRAVFNEEQVKRVFHGSFHKLHITVSPEKVKLNVDCQEVAEKPIKEANNITLEGYEVLGKMVKSGGGKRQSATFQLQMFDIVCSLSWISRDKCCDLPATRIESKCPALPHSCTCTQDSVGPQGPPGPSGSPGSKGPRGDRGDPGKPGAMGPRGEPGLQGAMGPPGPQGPNGLSIPGEPGRQGPKGDRGDSGLPGLQGPPGPRGPMGPVGQSGARGPPGKEGSSGPRGPPGPMGSPGNPGVPGMSGKPGKSGEPGSPGPVGLKGEKGERGDFASQNMMRSIARQVCEQLVSSQMSRIDGMLNQIPSGYRNGNPGPAGPPGPPGNQGPRGEPGQPGKSGFPGSPGLPGNQGERGLPGERGERGPPGADIRGQRGPPGPAGPPGESRTGPPGSPGSAGSRGPPGRQGNPGVRGPPGPPGYCDSSQCVGIPYNGQGYTGLA

Target ORF information:

RefSeq Version	XM_011490504.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes collagen type XII alpha 1 chain (col12a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490504.2

ORF Insert Sequence:

ATGAAGATAA GGCTGTCACT AGCCACGGCT GCCTTTCTGG TAGGCTTCTT AGCCTCCATA 
GATGCCCAAG TGGAGCCACC CTCAGACTTG AAATTTAAAA TTCTAAATGA AAACACAGTG 
GAAATGTCGT GGCTAAAGCC CTCATCAAGG ATAGAGGGCT TCAGAATACA AGTTGCGTCC 
GATGCAGATG AACCAAGCAG AGATTTCACC CTTGATGCAT ACTCAACCTC AACCTCCATC 
ACTAATCTGA CTCCTGATGT GGACTATTCT GTGTCAATAA GCTCCTATTA TGGTTCAGAG 
GAGAGCATCT CCATTTTTGG TCAACTGACT ATCCAGTCCA GTAACACCAC CAGACCAAGA 
CCAACATCAG ATGCACTCAA ATGTTCTGTC AGTGCAATAG CAGACTTGGT ATTTCTGGTG 
GACGGCTCAT GGAGTGTAGG CAGAGAGAAC TTCAAACACA TCCGGAGTTT CATTGGCTCG 
CTAGCAGGAG CCTTCGATAT CGGCGAGGAC AAGACTCGAG TGGCTGTGGT TCAGTACAGC 
ACAGATACCC GCACAGAATT CCCCCTGAAC CGTTACACAA GAAGAGGAGA CCTCCTTCAA 
GCCATCAACT CACTACCATA CAAAGGAGGA AACACCATGA CTGGTGATGC CATAGATTAC 
CTAATAAAAA ACATCTTCAC TGAGGCAGCT GGATCAAGGA AAGCCTTTCC AAAGGTGGCC 
ATGATCATAA CTGATGGAAA ATCCCAAGAC CCTGTAGAGG AGCATGCAAG AAGACTTAGG 
AACATCGGAG TGGAAATATT TGTCCTAGGT ATCAAAGGAG CAGATGAGGA TGAGCTCAGG 
GAAATTGCTT CCACGCCTCA TGCCAAACAT GTGTACAATG TGCCCAACTT TGATCTGATC 
CAAGAAGTGC AGAAAAGGAT CATCACAGAA GTCTGCTCTG GTGTTGATGA CCAACTCACC 
TCTTTAGTCA GTGGAGAAGA AATGGTGGAA GCCCCCTCTA ACCTCCAGGT CACAGAAATT 
GCATCAAAAT CAATGAGACT GACGTGGGAT GCATCCTTAG GGGACATCAC AGGCTACAAG 
CTTACCCTCA TTCCCCTGGT GCCTGGCATG AAACGGCAGG AACTCTACAT GGGAGCCACA 
CAGACATCTA TTAATGTCCG TGACCTTTCC CCAGAGACCG AGTATGATAT CAGCTTGTTT 
GCACTCAAAG GTCTAACTCC AAGCGAACCT CTCACAGATT TGGCAAAGAC CCAACCTGTC 
AAGGTGTTAA CAGAGTGTTC TATGGATGTG GATGTGCAGG CTGATGTGGT GCTGTTGGTT 
GATGGCTCAT ATAGCATTGG ATTACAAAAC TTTGCCAAAG TTCGTGAATT CCTGGAGGTC 
CTGGTCAAAT CCTTTGACAT TGGACCCGAC AAAATCCAGA TCAGCTTGGT CCAGTACAGT 
CGAGACCCAC ATACAGAGTT TTACCTGAAC ACCCACCATG ACATCAATGC TGTCGTTAAA 
GCTGTGCGCA ACTTCCCTTA TCGGGGCGGT TCCACAAACA CTGGCAAAGC CATGACCTAT 
GTCAGGGAAA AAATCTTTGT ACCAACTCGG GGAGCAAGGC AGAATGTGCC TCGTGTCATG 
GTCCTGATCA CAGACGGAAA GTCCTCTGAC TCCTTTAGGG TTGCTGCAAC CAAGCTAAGA 
AATATTGATG TGGAGATCTT TGCAGTTGGT GTGAAAGATG CAGTGAGATC AGAGCTGGAG 
GCCATTGCTA ATGAACCAGC AGACACTCAT GTCTATGAGG TGGAGGACTT TGATGCTTTT 
CAGAGGATTT CAAAAGAGCT AACTCAGTCC ATCTGTCTGA GGATTGAACA GGAACTGCTC 
AAGATCAAGA AGAAAAATTT ACTTCCACCC AGTAACTTGC AGTTCTCTGA GATTACATCG 
CGCAGCTTCC GTGCGACTTG GGTTGCACCT GCTGCTTCTG TCATGTCTTA TCTGGTACGA 
TACCGCAAAG CTGAAGACAT CACTGGAGAC TACATATCCC TGGCAGTACC TGGTCAAACC 
ACCACAACAG TTCTTCTTTA CCTCACTCCG ATCACCACTT ATGAAGTCAA CGTCTATGCT 
GAGTACGACA AGGGAGATAG TTTTGCGCTG ACTGGTCAAG AGACGACTCT GGAAGAACAA 
GGAACAGTGG AAAATCTCCG AGTCACAGAG GAGACAACTA ATAGTTTTAG GGTATCATGG 
CAAGCCGCCC CAGGTTCAGT CATTAGGTAT CGATTGTCGT ATGTTCCACT CAGTGGTATG 
GGAGAAATAC TCGAAGCTCA AACCCTTGGA CCAGAAACAA CCATAGTTCT CCAAGAGCTA 
TTCCCCATCA CCACCTACAG GGTGTCCGTC TCTCCTGAAT ATGCCGCTGG CGTTGGAGAT 
GAGTTACAAG TGGATGGTAC CACTAAGGAA GTTATTGGCT CACCACGAGA TCTTCGTGTC 
TTTGACGAGA CAATAAATAC TATGAAAGTT GCTTGGCAGG CTGCTCCAGG AAATGTAGTT 
CAGTACCACG TTACATACAA ACTCGCTGAA GGGGGTGATA TAAACATGGA ATTGGTCCAA 
GGAGATATCA CCCAAGCTTT GCTAAAGAAC CTCCAACCGG CCACTGAATA TGAGCTATTT 
GTCACAGCAC ACTACTCCAC TGGACAAGGC GACCCTCTGA TGGGTGCAGG AACCACTTTA 
GAAGAGCTTG GCTCACCTAG AGACTTGGAG ACCAGAGACG TCACTGACAC CAGTTTTGCC 
GTGTCTTGGA CTGCTGCTCC CGGTAATGTC CGTCAATACC GAATCAAGTG GAAGTCAATG 
TTCAGCGAGG AGACTGGAGA GAAAACTGTA GCAGGAGACA CCACTACGGC AGATTTGGAT 
GGTCTCACCC CAGAGACACG TTACCAAGTG TCTGTGTTCG CCCGCTATGG CCATGGAGAA 
GGCCAGCCAT TGGTTGGGGA AGAAACCACG GATATCTCAG CCAATGGTAG AGTAATTGTT 
GTCTCTGATG AGACGGAGAA GAGCATGAAA GTTACTTGGC AACCTGCACC TGGAAATGTA 
CTCAACTACC GCGTTACTTA CAAGCCGCAG TCAGGAGGGC GCCAGCTGGC CGCCAAAGTA 
CCTGGTGGCA ACACATCTAC AGTGTTGAGG CGACTTACTT CCCTGACCAC CTATGACATC 
ACAGTTGTAC CAGTGTACCG TTTCGGAGAA GGCAAAATAA GGCAAGGAGA AGGAACAACA 
TTGACACCCT ATAAAGGTCC AAGGAATCTC CAGACTTCAG AGCCCACCAA GACCAGTTTC 
AGAGTGACCT GGGACCATGC TCCAGGGGAC GTTAAGGGCT ATAAAGTTTC TTTCCATCCA 
GCTGAAGATG ACATTGATCT AAGAGAACTT TTAGTTAGTC CCCATGACAA CACAGTGGTG 
CTAGAGGAGC TCAGAGCTGG TACCAAATAC ACAGTGAGTG TGTTTGGCAT GTTTGACGGT 
GGGGAGAGTA TGCCTCTGGC TGGAGAGGAA AACACTACTT ACTCTGATGA TCCCGATTCC 
TCGCCTGTTG ATGTACCAAA AATGTCGTGC AAGACCAAAG CACAGGCAGA CATCGTGCTG 
CTGGTCGATG GCTCTTGGAG TATTGGACGT CTTAACTTCA AGACTATTAG GTCCTTCATT 
GCACGCATGG TGGGAGTCTT TGACATTGGT CCTGATCGGG TTCAGATTGG ACTTGCTCAG 
TATAGTGGAG ACCCAAAGAC AGAATGGCAC CTGAATGCTC ACCAAACCCG AGAGTCTCTT 
CTGGAAGCTG TAGCTAAGCT TCCCTACAAG GGAGGAAACA CCATGACAGG TCTGGCTTTG 
TACTATATCC TCCAGAACAA TTTCCAGGAG AGTGTCGGCA TGCGGCCCGG AGCTCGTAAG 
ATTGGTGTTT TGATCACTGA TGGCAAATCA CAGGATGAAA TCATCGCCAG CTCTCAGAGC 
TTGCGTGATG CGGGCATTGA GCTTTACGCC ATTGGTGTGA AGAACGCTGA TGAAAATGAG 
TTGAGGGCCA TTGCTAGTGA TCCCGATGAG ATTCACATGT ATAACGTGGC TGACTTCTCC 
TTCCTGCTGG ACATCGTGGA CACTCTGTCT GACAACCTCT GTAACAGCGT CACCGGATCA 
GATAATACTC CAGGTTCACC CACTAATCTG GTGACATCTG AGGTGACTCA CCAGTCTTTC 
CGAGCCACCT GGACCGGCCC TGAGGGCCCG GTGGAGAAGT ACCGCGTGGA GTACATGACT 
GTATCAGGAG CCCCTCAAGA GGTGCTTGTG GATGGGACTG TGACCACCGT GGTCCTCCGG 
GGACTCGATC CTCTCACAAA GTACCTGGTC AACGTTTACT CAGTGATTGG AGAGGAAAGC 
AGTGAGCCTC TGAAAGGAAC AGAGACCACA CTGCCTCTGA GTGCTGTGAG AGAGATGAGC 
GTTTATGACG AGCAGATGAC CACCATGAGG GCGAGATGGA AGGCGGTGCA GGGAGCCACG 
GGGTACCTGC TGACGTACCG TGCCACCAAT GCCACTCAGC CCACTGTTGA ACAGGAGATG 
AGAGTGGCAG CGGGTACAAC GGACGTCCAG CTGGTGAAGC TTACGCCCAA CACAGCTTAC 
TCGCTGTCCC TCTACGCCCT CTATGGACTA TCAGCCAGTG AAGCACTCCT CCGGCAGGGA 
GTCACCTTGC CAATGCCACC TGCAGGAGAG CTGAATGTTC GGGATGTCAC CCACAGCACC 
ATGGTGCTCT ACTGGGATGC TGCTCCCGGT GCAGTTCGCA AGTACATTAT CACCTACACT 
CCCGAGGACG GTGAAACTAA AGAACTGGAG GTTGGGGGAA GGATTACCAC CAAACTCCTG 
GAGAACCTCA TCTCACAGAC TGAGTACAGT TTAACTGTAA CTCCGGTTTA CGATGAAGGA 
CGTGGACAGG CGATGCTGGG AAGCGGAATC ACCGATGTGG TTCCAGCACC AAAGAACCTA 
CGATTCTCAG AAATTACACA GACCAGCTTC AGGGCCACCT GGGAGCACGG GGCCCCCGAT 
GTGGCTATGT ATCGAATTGC ATGGAGAAGG AAGGGAGAGA CCATCAATGA AGGCGAGGAA 
ATTCTGAGTG AGGAGGAGAC AACCTTTGTT CTGGAGAATC TGGATCCAGA TACGCCCTAT 
GACGTGTCTG TCACTGCAAT CTATCCAGAT GAATCAGAGA GTGAAGACCT GCTGGGCACT 
GAGAGGACAT TGTCCAAGTC AGATGGTAGA CCGATACCAA ATGCACCTCC CACCAATTTG 
GTTGTTTTCA ATGAGACCAC CACCACCCTG AATGCTAGGT GGACTCCACC TCTGGGTCGT 
GTCCAGAACT ACAGGATCAC TTATGTCCCC ACATCTGGAG GCCAGAGTCA GACTACCATG 
GTTGGAGCCA AGAAAAACAC AGTCCTGCTT CCAAAGCTGA CCCCTGACAC AGAGTACTCC 
ATCGGTGTTG TGGCGGTGTA CGCCAATGGA GTCAGCAGGG AGCTGAATGG CCTTGGAAAA 
ACCAAACCTT TGGGTGGTGT TAGAAACCTG CGTGTGACAA ACCCCACCAC GAGCACGTTG 
GATGTGAACT GGGAACCTGC TGAGGGTAAT GTGCGCCAGT ATAGGGTCTT CTATGTTCCT 
GCAGCTGGAG GAGAGCAGGA GATGATTCAG GTCTCCGGAA GCACCTACAA AGCAGTTCTG 
AAGAACCTGC AGTCGGACAC TGTCTACACT GTCACAGTGA CTCCTGTTTA TTCTACTGGA 
GAGGGGAAGC CCATGTCTGA AAACGGCAAA ACATTGGAGC GCAGTCCTGC TAGGAATATC 
CAGGTTTTCA ACCCCACAAC TAACACTTTG AATGTCCGCT GGGAAGCAGC CACCGGGCCG 
GTCCAGCAGT ACAGGGTGGT CTATGCTCCT TTGACTGGTG TGAGGCCAAC GGAGTCGATT 
TTGGTCCCCG GCACCACAAC CACAGCACTG CTGCAGCAGC TGGTCCCAGA CACAGCCTAC 
AATGTGGGAG TTGTTGCCTT GTACAGCGAT GGGGAAGGAA CCACTGTCAG CGATCAGGGA 
AAGACTTTGC CCCGGGGTGG TCCCAGAAAC ATGCGTGTGT ACGATCCCAC CACCAGTACG 
CTGTCTGTTA ACTGGGAACA CGCTGACGGT CCTGTTCTTC AGTACCGCAT CAGCTATGCC 
CCAACAACAG GAGACCCTAT CGAGGAATTT ACCACAGTTC AAGGGAACAG GAACAATGTG 
GTTCTTCAGA ATCTGGATCC AGACACTCCC TACAATATCA GAGTGACTGC TATGTATGCT 
GAAGGAGCGG GAGGAGAACT GGAGGGAGAT GGACGTACAG TTGGAATGCT CGGCCCCAGA 
AACCTGCGTG TATCTGATGA ATGGTACACT CGCTTCAGGG TGTCCTGGGA CCCCGCCCCC 
TCCGCAGTCA CTGGATACAA ACTCATCTAC CAGCCTGCGG GGTCTTATGA CTCATCTGAG 
GTGTTTGTTG GAGATGTGAC CACCTATCAG CTGCAAAACT TGAAGCCTGG AACTACCTAT 
GACTTGACGG TCCTAGCACA GTACAGAACG GGCATCAGTG GTCCTCTAAA TGGTCAAGGC 
ACCACATTGT ACCTCAATGT GACAGGCCTA ACCTCCTACA ATGTTGGCTA TGACTCCTTT 
TGCATCCAGT GGGTCCCTCA CCGAGCAGCC ACGTCGTACA GACTTAAAGT CAACCCTTTT 
GAGTCCTTCA AGGGTGGAGC TCAAGAAATC ACAGTTCAGG GCCCAGAGAG TAAATACTGC 
TTTGACAACC TGACCCCTGA CACTCTTTAC AACGCTACGG TTTACGCTCA GACCCTCAAC 
CTTGAAGGAC CTGGAGTCAG TATTAAGGAG AAAACATTGG TCAAACCCAC CGAGGTGCCA 
ACCGAGCCTC CCACCCCTCC CCCTCCATCA ATAGTGCCTG ATGCTCTTGA CATGTGTAAA 
GGTGCGAAGG CTGACTTGGT CTTCCTCATC GACGGCTCAT GGAGTATTGG AGATGACAGC 
TTTGCCAAAG TCCTCCAGTT TGTCACTAGT GTGACAGCAG CTTTTGATGT TGTTAGCCCA 
ACTGGAATGC AGGTTTCATT TGTGCAGTAT AGCGATGATG CTAAGACTGA GTTCAAACTG 
AACACCTACC ACAACAAGGG CATTGTCCTA TCTGCACTGA AGTCAATCCG CTACAGGGGA 
GGCAACACTA AGACTGGCAT TGCTCTGAAG CACGTCTATG AAAAGGTACT AACGTCCGAC 
AATGGCATGA GGAGGAATGT CCCGAAGGTG CTGGTTGTTG TCACAGATGG TCGTTCTCAA 
GATGAAGTCA AGAAGAGCGC AGAAAAACTT CAGCATTCTG GCTATAGTGT GTTTGTGGTA 
GGAGTGGCTG ATGTGAGCAT GAGAGAACTG GCAGTTATCG GCAGCAAGCC CACCGAAAGA 
CACGTGTTTG TCGTTGACGA TTACGACGCT TTTTCCAAAA TCCAGGACAA CTTGATCACA 
TTCATCTGTG AAACGGCTAC CTCCACTTGC CCTCTCATCT ACATCAATGG ATACACCACA 
CCTGGATTTA GGATGCTGGA AGCTTTCAAC ATCACAGACC GAACATTTGC TGGCATGAAT 
GGTGTCTCCA TGGAGCCAGG TTCTTTCAAC AGCTTCATTG CCTACAGGCT GCACAAAGAT 
GCCTTCCTAA ACCAGCCCAC AAAGGAAATC CACTCCGACG GCCTTCCTCC ATCCTACACT 
ATAATCCTGC TCCTCCGCCT GCTGCCTGAT ACACCCTCTG AACCTTTTGA TATCTGGCAG 
ATCTCTGACA AAAACAACAA CCCTGAAGTT GGAGTCACTA TGAATCCTTC CAGCAAAACA 
CTCACGTTCT ACAACAAGGA CACCCGGGGA GAGATCCAGA GAGCCGTGTT CAATGAGGAG 
CAAGTGAAAC GGGTTTTCCA CGGAAGCTTC CACAAGCTCC ACATTACCGT CTCCCCAGAG 
AAGGTCAAGC TCAACGTGGA CTGCCAAGAG GTGGCAGAGA AGCCAATCAA GGAGGCCAAC 
AACATCACGC TGGAGGGCTA TGAAGTGCTT GGCAAGATGG TCAAATCAGG AGGTGGAAAG 
AGGCAGTCCG CTACATTCCA GCTCCAGATG TTTGACATTG TCTGCAGCTT GAGTTGGATC 
AGCCGTGACA AATGCTGTGA CCTGCCTGCT ACGAGAATTG AGTCCAAATG TCCTGCTCTT 
CCCCACTCCT GCACATGCAC CCAAGACAGT GTTGGGCCCC AGGGGCCTCC TGGGCCTTCG 
GGCAGCCCAG GGTCTAAAGG ACCCAGAGGA GACAGGGGAG ACCCAGGCAA ACCTGGTGCA 
ATGGGTCCAC GAGGTGAGCC AGGACTCCAA GGTGCAATGG GCCCACCAGG CCCACAAGGC 
CCTAATGGAC TGTCTATCCC CGGAGAACCG GGTCGCCAAG GACCAAAGGG AGATCGTGGA 
GACTCAGGCC TGCCTGGGCT GCAAGGTCCT CCTGGGCCGC GTGGTCCTAT GGGTCCTGTC 
GGACAAAGTG GAGCACGGGG GCCACCAGGA AAGGAAGGAT CATCTGGACC CAGAGGCCCA 
CCAGGGCCCA TGGGAAGTCC AGGAAATCCA GGTGTACCTG GTATGTCTGG AAAACCAGGA 
AAGTCAGGAG AACCAGGAAG TCCAGGACCT GTTGGCCTTA AAGGAGAAAA GGGAGAGCGG 
GGAGACTTTG CATCCCAAAA TATGATGCGC TCCATTGCCA GACAAGTTTG TGAACAGCTC 
GTGAGCAGTC AGATGAGCAG AATTGACGGG ATGCTCAATC AAATTCCTTC TGGATATCGT 
AATGGCAATC CAGGCCCGGC TGGTCCTCCT GGTCCCCCTG GCAACCAAGG ACCTCGTGGA 
GAGCCCGGAC AACCTGGAAA ATCTGGTTTC CCTGGAAGCC CAGGATTACC TGGAAATCAA 
GGAGAAAGAG GGTTACCAGG AGAGAGGGGG GAGAGAGGAC CTCCAGGAGC AGATATCAGA 
GGACAAAGAG GTCCACCTGG ACCAGCAGGT CCACCAGGGG AGTCTAGAAC AGGTCCCCCA 
GGTTCTCCTG GCTCTGCTGG ATCCCGTGGC CCCCCAGGTC GTCAAGGTAA TCCAGGTGTG 
AGAGGACCAC CAGGACCCCC TGGATACTGT GACTCCTCTC AGTGTGTTGG TATTCCTTAC 
AATGGACAAG GATACACAGG TTTGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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col12a1 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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