pkd2 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	pkd2
Entrez Gene ID	100192346
Full Name	polycystin 2, transient receptor potential cation channel
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001136518.1	NP_001129990.1	polycystin-2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Pkd1l1 complexes with Pkd2 on motile cilia and functions to establish the left-right axis.
Kamura K, Kobayashi D, Uehara Y, Koshida S, Iijima N, Kudo A, Yokoyama T, Takeda H
Development (Cambridge, England)138(6)1121-9(2011 Mar)

The medaka draft genome and insights into vertebrate genome evolution.
Kasahara M, Naruse K, Sasaki S, Nakatani Y, Qu W, Ahsan B, Yamada T, Nagayasu Y, Doi K, Kasai Y, Jindo T, Kobayashi D, Shimada A, Toyoda A, Kuroki Y, Fujiyama A, Sasaki T, Shimizu A, Asakawa S, Shimizu N, Hashimoto S, Yang J, Lee Y, Matsushima K, Sugano S, Sakaizumi M, Narita T, Ohishi K, Haga S, Ohta F, Nomoto H, Nogata K, Morishita T, Endo T, Shin-I T, Takeda H, Morishita S, Kohara Y
Nature447(7145)714-9(2007 Jun)

Fish mesonephric model of polycystic kidney disease in medaka (Oryzias latipes) pc mutant.
Mochizuki E, Fukuta K, Tada T, Harada T, Watanabe N, Matsuo S, Hashimoto H, Ozato K, Wakamatsu Y
Kidney international68(1)23-34(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pkd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pkd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJa00042	NM_001136518.1 Latest version!	Oryzias latipes polycystin 2, transient receptor potential cation channel (pkd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pkd2 ( NM_001136518.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> NP_001129990.1 Oryzias latipes polycystin 2, transient receptor potential cation channel (pkd2), mRNA.

Price & Availability↑

CloneID	OJa00042
Clone ID Related Accession (Same CDS sequence)	NM_001136518.1
Accession Version	NM_001136518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550505600000
Organism	Oryzias latipes(Japanese medaka)
Product	polycystin-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB365080.1. ##Evidence-Data-START## Transcript exon combination :: AB365080.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00029877, SAMD00029878 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGCGA CCCGAGTCCG ACCACAGAGC CAGGCTGGCA CTGACAAGCC GCCCCGCACG 
CTCGACTCCA GCGAGGGGAT AGAGATGGAG AACATCCAGC ATCAAGATCC GGGCCTCGGG 
GGAGTCGCAG GCTCGCCGTC CCCGCCGTCC AGACAGGCGT GGAGCCGGGA TAACCCGGTC 
TTTGAACCAG AGGAGGAAAT CGTGCAAGCA GACTGGCCTC AAGCAAGTCC CGGCAGGAGG 
TCTGCGTTCA CGGCCTCCGG CGGCAGCAGC TGCAGCAGCG GCCATGGCGG CTACACCCGC 
GCGGGCAGTA GCACTCAAAT CCCCCGAGGA GGACTGTATC CCACCCAGAC CCTGAATGGC 
CAGCAGCAAG ACAGACACGA GCATCCCGGC TGTATGAAGC GGATATTTCA AAATATCAGA 
ATTCTGTGGG GGACAGAGTT GATGGAGGAC GGTGACAGCA ACCGTGAAAG GTACCTCTGG 
AACGTACTGA GAGAGCTGCT CACATATATC GCGTTTCTCA TCACCATTTG TATCTTGACC 
TATGGGATGA TAAGTACCGA CATGTATTAT TACACCAAAG CCATGTCTCA GCTTTTCCTC 
GACGCGCCAC TGTCTTCAGA AAACCCCAAG ACTTTCAGGA GCCTCTCCAC CATGGAGGAT 
TTCTGGAAGT ATACTCAGGG GCCCTTCCTC GGGGGCATGT ATTGGGAGGT GTGGTACAAC 
AACCAGAGCC TGCCAGGGAA CCAGAGTTTC ATCTACTATG AGAACATCCT CCTCGGGGTG 
CCGCGACTCC GCCAGGTCAG AGTCCACAAC GAGACCTGCT CCATCCACGA AGATCTCAGG 
GATGAAGTTC AAGGTTGCTA CGGTGTATAC ACCCCCAGCA ATGGGGACAA CTCTCCCTTT 
GGCCCTCAAA ACGGAACAGC TTGGGTGCAC ACTACAGAAA GCAAGATGAA CGCCAGCACT 
CATTGGGGTC AGGTGTCCAA ATATGGAGGT GGAGGCTACT ACCAAGACCT GTCCCGCACC 
AGAGAAGAGT CCGGCCTCCA ACTGCAGTTT CTCAAAGACC ACCTGTGGCT GGATAGAGGC 
ACCAGAGCCA TCTTTCTTGA CTTTTCAGTC TACAACGGAA ACGTCAACCT TGTCTGTATA 
GCCAGGTTAT TGGTGGAGTT CCCAGCTACT GGTGGAGTGT TGACCTCTTG GCAGTTCCAA 
ACAGTGCGCC TGATAAAGTA CGTGTCCAGC TGGGACTACT TTGTTGGCGT GTGTGAGGTG 
GCGTTCTGTC TGTTCGTCCT CTACTACGTG GTGGAGGAAG TGCTGGAAAT CCACATTCAC 
CGACTGCATT ATTTCAAGAA CCTGTGGAAT TGTCTGGATG TTCTCATTGT GACGTTAAGT 
GTTGTTGCCA TTATCATGAA CATAACCAGA GCAGCCATGG GTGGAGACCT CCTGAAAGGC 
TTGGAGAACT ACACCTCTCA CACCAGCTTT GATTCTCTGG CTAACCTGCA GGTCCAGTTC 
AACAACATGG CTGCAGTGAT AGTCTTTTTC TGCTGGGTAA AGCTTTTTAA GTTCATCAAC 
TTCAACAAGA CCATGAGTCA GCTGTCCACC ACCATGTCCC GCTGTGCCAA GGACCTCGTG 
GGTTTTGCCA TCATGTTCTT CATCATTTTC CTGGCTTATG CTCAGCTTGC CTACCTGGTG 
TTTGGAACTC AGGTCAATGA TTTCAGCACT TTCCAAGACA GCGTGTTTAC CCAGTTTCGT 
GTCATTTTGG GAGACTTTGA CTTTTTCGAG ATCAAAGAAG CGAACCCAGT TCTCGGACCG 
ATTTACTTTA TAACTTTCGT CCTCTTCATT TTCTTTATTC TCATGAACAT GTTCCTGGCC 
ATCATTAATG ATACGTACTC TGAGGTGAAG GCGGACATGG CCCAGCACAG GTCTGAGATG 
GAAATGACCG ACTTCATCAA GAAGGGCTGT ACCAAAGCTT TGGTCAAGTT GAGACTGAAG 
AAGACGACAG TGGACGACAT ATCTGACAGT TTGCGTCAAG CCGGGGGAAA GCTGAACTAC 
GATGAACTCC TTCAGGATCT CAAAGAAAAG GGCCACACGG AAGCAGAGAT TCAGGCCATC 
TTTGCAAAGT ACGACCAGGA CGGGGATCTG GAGTTGACGG AGCACGACCA CCAACAAATG 
AGAGATGACC TGGAGAAAGA GAGAGAGGAC TTGGATCTGG AACGCAACTC ACTGACCAGA 
CCCAGCAGTG GGAGGAGCTT CCCTCGTACA CAAGATGACT CAGAGGAAGA CGATGATGAG 
GACTCTGGTC ACAGCTCTCG TCGCCGTGGC AGCAGTTCGG GAGGCGTCTC CTATGAGGAG 
TTTCAAGTGC TTGTGAGGCG CGTGGACAGA ATGGAGAATT CGATCGGCAG CATCGTGTCC 
AAGATCGACG CTGTGATTGT GAAGCTGGAG GGCATGGAGA GAGCCAAGCT GAAAAGACGA 
GACGTGCTGG TCCGGCTGCT GGATGGCGTC ATGGAGGACG AGCGTTTGGG CCGGGACGCA 
GACACCCACA GAGAGCAGAT GAAGAGGCTG GTGAAGGAGG AGCTGGAGCG CTGGGAGACG 
GACGACGTGG CCTCGCAGGT CAGTCACCCT CAGCCCGCCA CCCCCATCGG CCCTCGGCCC 
CGACCCCCCT CCTCCCTGTC CACAGACGGC CTCGACACGA GCGCTAACGG AGGAACCCAC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129990.1
CDS	444..3149
Translation	MSATRVRPQSQAGTDKPPRTLDSSEGIEMENIQHQDPGLGGVAGSPSPPSRQAWSRDNPVFEPEEEIVQADWPQASPGRRSAFTASGGSSCSSGHGGYTRAGSSTQIPRGGLYPTQTLNGQQQDRHEHPGCMKRIFQNIRILWGTELMEDGDSNRERYLWNVLRELLTYIAFLITICILTYGMISTDMYYYTKAMSQLFLDAPLSSENPKTFRSLSTMEDFWKYTQGPFLGGMYWEVWYNNQSLPGNQSFIYYENILLGVPRLRQVRVHNETCSIHEDLRDEVQGCYGVYTPSNGDNSPFGPQNGTAWVHTTESKMNASTHWGQVSKYGGGGYYQDLSRTREESGLQLQFLKDHLWLDRGTRAIFLDFSVYNGNVNLVCIARLLVEFPATGGVLTSWQFQTVRLIKYVSSWDYFVGVCEVAFCLFVLYYVVEEVLEIHIHRLHYFKNLWNCLDVLIVTLSVVAIIMNITRAAMGGDLLKGLENYTSHTSFDSLANLQVQFNNMAAVIVFFCWVKLFKFINFNKTMSQLSTTMSRCAKDLVGFAIMFFIIFLAYAQLAYLVFGTQVNDFSTFQDSVFTQFRVILGDFDFFEIKEANPVLGPIYFITFVLFIFFILMNMFLAIINDTYSEVKADMAQHRSEMEMTDFIKKGCTKALVKLRLKKTTVDDISDSLRQAGGKLNYDELLQDLKEKGHTEAEIQAIFAKYDQDGDLELTEHDHQQMRDDLEKEREDLDLERNSLTRPSSGRSFPRTQDDSEEDDDEDSGHSSRRRGSSSGGVSYEEFQVLVRRVDRMENSIGSIVSKIDAVIVKLEGMERAKLKRRDVLVRLLDGVMEDERLGRDADTHREQMKRLVKEELERWETDDVASQVSHPQPATPIGPRPRPPSSLSTDGLDTSANGGTHV

Target ORF information:

RefSeq Version	NM_001136518.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes polycystin 2, transient receptor potential cation channel (pkd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136518.1

ORF Insert Sequence:

ATGAGTGCGA CCCGAGTCCG ACCACAGAGC CAGGCTGGCA CTGACAAGCC GCCCCGCACG 
CTCGACTCCA GCGAGGGGAT AGAGATGGAG AACATCCAGC ATCAAGATCC GGGCCTCGGG 
GGAGTCGCAG GCTCGCCGTC CCCGCCGTCC AGACAGGCGT GGAGCCGGGA TAACCCGGTC 
TTTGAACCAG AGGAGGAAAT CGTGCAAGCA GACTGGCCTC AAGCAAGTCC CGGCAGGAGG 
TCTGCGTTCA CGGCCTCCGG CGGCAGCAGC TGCAGCAGCG GCCATGGCGG CTACACCCGC 
GCGGGCAGTA GCACTCAAAT CCCCCGAGGA GGACTGTATC CCACCCAGAC CCTGAATGGC 
CAGCAGCAAG ACAGACACGA GCATCCCGGC TGTATGAAGC GGATATTTCA AAATATCAGA 
ATTCTGTGGG GGACAGAGTT GATGGAGGAC GGTGACAGCA ACCGTGAAAG GTACCTCTGG 
AACGTACTGA GAGAGCTGCT CACATATATC GCGTTTCTCA TCACCATTTG TATCTTGACC 
TATGGGATGA TAAGTACCGA CATGTATTAT TACACCAAAG CCATGTCTCA GCTTTTCCTC 
GACGCGCCAC TGTCTTCAGA AAACCCCAAG ACTTTCAGGA GCCTCTCCAC CATGGAGGAT 
TTCTGGAAGT ATACTCAGGG GCCCTTCCTC GGGGGCATGT ATTGGGAGGT GTGGTACAAC 
AACCAGAGCC TGCCAGGGAA CCAGAGTTTC ATCTACTATG AGAACATCCT CCTCGGGGTG 
CCGCGACTCC GCCAGGTCAG AGTCCACAAC GAGACCTGCT CCATCCACGA AGATCTCAGG 
GATGAAGTTC AAGGTTGCTA CGGTGTATAC ACCCCCAGCA ATGGGGACAA CTCTCCCTTT 
GGCCCTCAAA ACGGAACAGC TTGGGTGCAC ACTACAGAAA GCAAGATGAA CGCCAGCACT 
CATTGGGGTC AGGTGTCCAA ATATGGAGGT GGAGGCTACT ACCAAGACCT GTCCCGCACC 
AGAGAAGAGT CCGGCCTCCA ACTGCAGTTT CTCAAAGACC ACCTGTGGCT GGATAGAGGC 
ACCAGAGCCA TCTTTCTTGA CTTTTCAGTC TACAACGGAA ACGTCAACCT TGTCTGTATA 
GCCAGGTTAT TGGTGGAGTT CCCAGCTACT GGTGGAGTGT TGACCTCTTG GCAGTTCCAA 
ACAGTGCGCC TGATAAAGTA CGTGTCCAGC TGGGACTACT TTGTTGGCGT GTGTGAGGTG 
GCGTTCTGTC TGTTCGTCCT CTACTACGTG GTGGAGGAAG TGCTGGAAAT CCACATTCAC 
CGACTGCATT ATTTCAAGAA CCTGTGGAAT TGTCTGGATG TTCTCATTGT GACGTTAAGT 
GTTGTTGCCA TTATCATGAA CATAACCAGA GCAGCCATGG GTGGAGACCT CCTGAAAGGC 
TTGGAGAACT ACACCTCTCA CACCAGCTTT GATTCTCTGG CTAACCTGCA GGTCCAGTTC 
AACAACATGG CTGCAGTGAT AGTCTTTTTC TGCTGGGTAA AGCTTTTTAA GTTCATCAAC 
TTCAACAAGA CCATGAGTCA GCTGTCCACC ACCATGTCCC GCTGTGCCAA GGACCTCGTG 
GGTTTTGCCA TCATGTTCTT CATCATTTTC CTGGCTTATG CTCAGCTTGC CTACCTGGTG 
TTTGGAACTC AGGTCAATGA TTTCAGCACT TTCCAAGACA GCGTGTTTAC CCAGTTTCGT 
GTCATTTTGG GAGACTTTGA CTTTTTCGAG ATCAAAGAAG CGAACCCAGT TCTCGGACCG 
ATTTACTTTA TAACTTTCGT CCTCTTCATT TTCTTTATTC TCATGAACAT GTTCCTGGCC 
ATCATTAATG ATACGTACTC TGAGGTGAAG GCGGACATGG CCCAGCACAG GTCTGAGATG 
GAAATGACCG ACTTCATCAA GAAGGGCTGT ACCAAAGCTT TGGTCAAGTT GAGACTGAAG 
AAGACGACAG TGGACGACAT ATCTGACAGT TTGCGTCAAG CCGGGGGAAA GCTGAACTAC 
GATGAACTCC TTCAGGATCT CAAAGAAAAG GGCCACACGG AAGCAGAGAT TCAGGCCATC 
TTTGCAAAGT ACGACCAGGA CGGGGATCTG GAGTTGACGG AGCACGACCA CCAACAAATG 
AGAGATGACC TGGAGAAAGA GAGAGAGGAC TTGGATCTGG AACGCAACTC ACTGACCAGA 
CCCAGCAGTG GGAGGAGCTT CCCTCGTACA CAAGATGACT CAGAGGAAGA CGATGATGAG 
GACTCTGGTC ACAGCTCTCG TCGCCGTGGC AGCAGTTCGG GAGGCGTCTC CTATGAGGAG 
TTTCAAGTGC TTGTGAGGCG CGTGGACAGA ATGGAGAATT CGATCGGCAG CATCGTGTCC 
AAGATCGACG CTGTGATTGT GAAGCTGGAG GGCATGGAGA GAGCCAAGCT GAAAAGACGA 
GACGTGCTGG TCCGGCTGCT GGATGGCGTC ATGGAGGACG AGCGTTTGGG CCGGGACGCA 
GACACCCACA GAGAGCAGAT GAAGAGGCTG GTGAAGGAGG AGCTGGAGCG CTGGGAGACG 
GACGACGTGG CCTCGCAGGT CAGTCACCCT CAGCCCGCCA CCCCCATCGG CCCTCGGCCC 
CGACCCCCCT CCTCCCTGTC CACAGACGGC CTCGACACGA GCGCTAACGG AGGAACCCAC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Pkd1l1 complexes with Pkd2 on motile cilia and functions to establish the left-right axis.
Development (Cambridge, England)138(6)1121-9(2011 Mar)
Kamura K,Kobayashi D,Uehara Y,Koshida S,Iijima N,Kudo A,Yokoyama T,Takeda H

The medaka draft genome and insights into vertebrate genome evolution.
Nature447(7145)714-9(2007 Jun)
Kasahara M,Naruse K,Sasaki S,Nakatani Y,Qu W,Ahsan B,Yamada T,Nagayasu Y,Doi K,Kasai Y,Jindo T,Kobayashi D,Shimada A,Toyoda A,Kuroki Y,Fujiyama A,Sasaki T,Shimizu A,Asakawa S,Shimizu N,Hashimoto S,Yang J,Lee Y,Matsushima K,Sugano S,Sakaizumi M,Narita T,Ohishi K,Haga S,Ohta F,Nomoto H,Nogata K,Morishita T,Endo T,Shin-I T,Takeda H,Morishita S,Kohara Y

Fish mesonephric model of polycystic kidney disease in medaka (Oryzias latipes) pc mutant.
Kidney international68(1)23-34(2005 Jul)
Mochizuki E,Fukuta K,Tada T,Harada T,Watanabe N,Matsuo S,Hashimoto H,Ozato K,Wakamatsu Y

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

pkd2 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM