LOC110536077 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110536077
Entrez Gene ID	110536077
Full Name	smoothelin-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021621610.1	XP_021477285.1	smoothelin-like isoform X2
XM_021621616.1	XP_021477291.1	smoothelin-like isoform X7
XM_021621609.1	XP_021477284.1	smoothelin-like isoform X1
XM_021621611.1	XP_021477286.1	smoothelin-like isoform X3
XM_021621615.1	XP_021477290.1	smoothelin-like isoform X6
XM_021621613.1	XP_021477288.1	smoothelin-like isoform X4
XM_021621614.1	XP_021477289.1	smoothelin-like isoform X5

Pathways from BioSystems
Gene Ontology

The following LOC110536077 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110536077 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOf56964	XM_021621615.1 Latest version!	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOf56959	XM_021621609.1 Latest version!	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOf56960	XM_021621610.1 Latest version!	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOf56961	XM_021621611.1 Latest version!	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOf56962	XM_021621613.1 Latest version!	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOf56963	XM_021621614.1 Latest version!	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOf56965	XM_021621616.1 Latest version!	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110536077 ( XM_021621615.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021477290.1 Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OOf56964
Clone ID Related Accession (Same CDS sequence)	XM_021621615.1
Accession Version	XM_021621615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	smoothelin-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTGG TTCTGGACCC CCTGGTGAGG GATGAGGTCC CGGGCCCTCT GCTGACCCGC 
CCACCGACCC GGGACCCTGT GACATCATCA GAGCCGGACG TGGTCCTATC CCACCGCCAG 
CGTTCTGACT CGACATCCTC AGAACGCAGC CTGGCCTCCA GGCCCCGTCT GGACTCTGGA 
GCCTCAGAGA AGAGTCTGGG TCTGGGCTCA GCCTTTCGGA CCAGGGTGGA CTCAGGAGCA 
TCAGAAAAGA GCTTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCAG GACCAGGGTG GACTCTGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCAGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
CGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCTG GAGCATCAGA AAAGAGCCTG GGCTCAGCCT TCAGGACCAG GGTGGACTCA 
GGAGCATCAG ACAGAAGCCA GTGTTCCGCC CACAGAGGCA GACTGGACTC TGGGGCCTCA 
GAGTCCCACT TCAGACAGAG ACTGGACTCT GGCACATCAG ACTCCAGTAT GGGACCGCCA 
GCCCCCCTGG GCTCTGAGAC CTCCGACGGC GCAGGGCTCC TGTCACGGAA ACGGTTGGAC 
TCAGGGACGT CTGACCTGAG TGTGGGCGTG TTGTCTGTGG GCCGGAGCCG TGTAGAGCAG 
CCAGAGGTGA CCATGAGTAG CTCCTCCTCC AACACAGACT CTGAGGCTGA AAGCACCAAC 
CACCATCCAG ACCAGCCCCA TCAGGACAGC ACCACTACCA ACCATTATCA GCCTGATGGG 
GCCGTCCTAT TCAGGAAAGC CCCAGACTCC CACAGTGGCC TGCTCAATGG CAGTGCCAAG 
GAAAGAGGAG ACATCCAGGA ACAGAAGGTC AGATATACTT TACTCACTCC TCTCAGAAAA 
TCAAATGATG CAGTTCCTGA GAAGAAAGAG CCGGTTGTCT TGGCCCCGTT CGGCCGGGCT 
AACTCTGTGC GGGACTGTAT GCGTAAGTTC ACGGAGCCCA GTCCCAGTGT CCCGGTCCTG 
AAGAGGGGCT CTCAGTCTCT GAGGAACGCG ACAGCCCCCA GTGCCCGAGT CCTAGGCAGA 
GCCACCCAGC TGAGTGAGGG GCCAGCAGCA GGGACAGCCA AGCAGACAGC AGTGACCACA 
GTAGTATCCA TCACCACCCC TACAGACAGA CCCGGGAGAC CCCATGTAGG CTCTACATCC 
TACTCTTCAT CCGCCTCCTC CTCAAATAAC CATGTCCAAT GCCAGGGCAC AGTTCATCCA 
GGGGGCGTGG CCACCAAACC TCTGCAAAGC GCTGCTCAAT CCTTGAACGC TGTAGGCGGG 
GCAAAGCAGG CGGCTGAAAA GCTGCGGCAC GCCTCAGGCG GCTCAAAGGA AGACAAGACC 
CCAGGGAGAG CATCTGCACA GCGGGACAGC AAGGGAGCGG CAGACCCCGA CATGAAGACC 
TTCCTAACTA TCGAGATCAA AGATGGGCGC ACCACCACCA CCTCTTCATC CTCTAGAGGC 
AACCTGGCCC CACGCATCTC CACCAACCCC ATAGGTCAGA GAGCAGAGTT GACCCTGGGC 
CTCAGAGCGA CCCCCTTCAA GATGTCCTCC TCCAGCCTTA CCTACGGATC CTCTTTCAAG 
ATGGCCTCAG AGCCCGTGTT GGCAGTGGAG CCCTCAGTGC GGGTGGCCTG CTGTCAGCCC 
CCGGCTGCTG TACCCAACGG CTCCTATGCG CCACCTCGGG ACAAATTCGA AGAGTGCTCC 
AGAAAACTGA CCGCAGAACA GCTGGCCGCC ATCGACGATG AGGAGCTCCT GGATAAAATG 
CTGGATGAGT CCAAGGACTT TGAGGAGAGG AAGATGATCC GACAGGCCAT GAGAGATCTG 
CGTAAGAGGA AGAGAGAGGC CGAACTGGGA TGTACTCAAG AGGAAATAGA CCAGAGAGAA 
AAGGAGCGGG AGTTTCGTCT TGCGGAGCTT CGGCAGCAGA GAGCGGAGCA TACTCAGAAG 
GGTCGTACGG GAGGAGGAGC TGGAGAGGTG GTCATGAGGA AGGTGGAGAT GTCAGCTGAT 
GGCTCCACCC TCAGCCAAGT CACCAAGACA GACTGCTTTG CCCAGTCTGG TGATGGCAGC 
AGGACAGCTC ACAGCACCAT CATGGAGGCC AGTTATGTGA AGAAAACAGA CGTGGGGACA 
GTTCAGTCCA AGTCCTACAG CTGCTCCTCC TCTACCTCCA GCAGCAGCTC CACCAGGAAA 
GTGGGCAGTG TGTTTGACCG CGAGGACGAC TCGGTGTCCC GTGCTGCAGA GCGCCGGCAG 
GCAGAGAAGC GCAAGGAGCT GATGAGAGCC CAGACTCTGC CCAAGATGTC CGCCGCACAG 
TCCCGCAAAG CCATGATTGA GAAACTGGAC AAGGAGAGCG GAGGGCCAGG AAATAAAGCA 
GTTGCCAAGG TGAACACAGT CCAGCGCTCA GCTAGCTGCA TGGTGCCCAA TGCCAACTCC 
ATCAAACAGA TGCTCCTGGA CTGGTGCCGC GCCAAGACCC GCTCATACCA GAATGTGGAC 
ATCCAGAACT TCTCGTCCAG TTGGAGTGAT GGCATGGCGT TCTGTGCCCT GGTCCATAAC 
TTCTTCCCAG AGGCCTTTGA CTACTCCTCT CTCACCCCTG CCAACCGCAG ACACAACTTT 
GAGGTGGCCT TCAGCACTGC AGAGGAATGT GCCAACTGTA TGCCCCTGTT GGAGGTAGAG 
GACATGATGA TCATGGGGAG GAAGCCTGAC TCCAAGTGTG TGTTCACCTA TGTGCAGTCA 
CTGGTCAATC ACCTGCGCAG GCATGAGATG AAACTCGCCA AACTCCAGGG CACCAACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021477290.1
CDS	208..3150
Translation	MVLVLDPLVRDEVPGPLLTRPPTRDPVTSSEPDVVLSHRQRSDSTSSERSLASRPRLDSGASEKSLGLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASDRSQCSAHRGRLDSGASESHFRQRLDSGTSDSSMGPPAPLGSETSDGAGLLSRKRLDSGTSDLSVGVLSVGRSRVEQPEVTMSSSSSNTDSEAESTNHHPDQPHQDSTTTNHYQPDGAVLFRKAPDSHSGLLNGSAKERGDIQEQKVRYTLLTPLRKSNDAVPEKKEPVVLAPFGRANSVRDCMRKFTEPSPSVPVLKRGSQSLRNATAPSARVLGRATQLSEGPAAGTAKQTAVTTVVSITTPTDRPGRPHVGSTSYSSSASSSNNHVQCQGTVHPGGVATKPLQSAAQSLNAVGGAKQAAEKLRHASGGSKEDKTPGRASAQRDSKGAADPDMKTFLTIEIKDGRTTTTSSSSRGNLAPRISTNPIGQRAELTLGLRATPFKMSSSSLTYGSSFKMASEPVLAVEPSVRVACCQPPAAVPNGSYAPPRDKFEECSRKLTAEQLAAIDDEELLDKMLDESKDFEERKMIRQAMRDLRKRKREAELGCTQEEIDQREKEREFRLAELRQQRAEHTQKGRTGGGAGEVVMRKVEMSADGSTLSQVTKTDCFAQSGDGSRTAHSTIMEASYVKKTDVGTVQSKSYSCSSSTSSSSSTRKVGSVFDREDDSVSRAAERRQAEKRKELMRAQTLPKMSAAQSRKAMIEKLDKESGGPGNKAVAKVNTVQRSASCMVPNANSIKQMLLDWCRAKTRSYQNVDIQNFSSSWSDGMAFCALVHNFFPEAFDYSSLTPANRRHNFEVAFSTAEECANCMPLLEVEDMMIMGRKPDSKCVFTYVQSLVNHLRRHEMKLAKLQGTNL

Target ORF information:

RefSeq Version	XM_021621615.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621615.1

ORF Insert Sequence:

ATGGTTCTGG TTCTGGACCC CCTGGTGAGG GATGAGGTCC CGGGCCCTCT GCTGACCCGC 
CCACCGACCC GGGACCCTGT GACATCATCA GAGCCGGACG TGGTCCTATC CCACCGCCAG 
CGTTCTGACT CGACATCCTC AGAACGCAGC CTGGCCTCCA GGCCCCGTCT GGACTCTGGA 
GCCTCAGAGA AGAGTCTGGG TCTGGGCTCA GCCTTTCGGA CCAGGGTGGA CTCAGGAGCA 
TCAGAAAAGA GCTTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCAG GACCAGGGTG GACTCTGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCAGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
CGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCTG GAGCATCAGA AAAGAGCCTG GGCTCAGCCT TCAGGACCAG GGTGGACTCA 
GGAGCATCAG ACAGAAGCCA GTGTTCCGCC CACAGAGGCA GACTGGACTC TGGGGCCTCA 
GAGTCCCACT TCAGACAGAG ACTGGACTCT GGCACATCAG ACTCCAGTAT GGGACCGCCA 
GCCCCCCTGG GCTCTGAGAC CTCCGACGGC GCAGGGCTCC TGTCACGGAA ACGGTTGGAC 
TCAGGGACGT CTGACCTGAG TGTGGGCGTG TTGTCTGTGG GCCGGAGCCG TGTAGAGCAG 
CCAGAGGTGA CCATGAGTAG CTCCTCCTCC AACACAGACT CTGAGGCTGA AAGCACCAAC 
CACCATCCAG ACCAGCCCCA TCAGGACAGC ACCACTACCA ACCATTATCA GCCTGATGGG 
GCCGTCCTAT TCAGGAAAGC CCCAGACTCC CACAGTGGCC TGCTCAATGG CAGTGCCAAG 
GAAAGAGGAG ACATCCAGGA ACAGAAGGTC AGATATACTT TACTCACTCC TCTCAGAAAA 
TCAAATGATG CAGTTCCTGA GAAGAAAGAG CCGGTTGTCT TGGCCCCGTT CGGCCGGGCT 
AACTCTGTGC GGGACTGTAT GCGTAAGTTC ACGGAGCCCA GTCCCAGTGT CCCGGTCCTG 
AAGAGGGGCT CTCAGTCTCT GAGGAACGCG ACAGCCCCCA GTGCCCGAGT CCTAGGCAGA 
GCCACCCAGC TGAGTGAGGG GCCAGCAGCA GGGACAGCCA AGCAGACAGC AGTGACCACA 
GTAGTATCCA TCACCACCCC TACAGACAGA CCCGGGAGAC CCCATGTAGG CTCTACATCC 
TACTCTTCAT CCGCCTCCTC CTCAAATAAC CATGTCCAAT GCCAGGGCAC AGTTCATCCA 
GGGGGCGTGG CCACCAAACC TCTGCAAAGC GCTGCTCAAT CCTTGAACGC TGTAGGCGGG 
GCAAAGCAGG CGGCTGAAAA GCTGCGGCAC GCCTCAGGCG GCTCAAAGGA AGACAAGACC 
CCAGGGAGAG CATCTGCACA GCGGGACAGC AAGGGAGCGG CAGACCCCGA CATGAAGACC 
TTCCTAACTA TCGAGATCAA AGATGGGCGC ACCACCACCA CCTCTTCATC CTCTAGAGGC 
AACCTGGCCC CACGCATCTC CACCAACCCC ATAGGTCAGA GAGCAGAGTT GACCCTGGGC 
CTCAGAGCGA CCCCCTTCAA GATGTCCTCC TCCAGCCTTA CCTACGGATC CTCTTTCAAG 
ATGGCCTCAG AGCCCGTGTT GGCAGTGGAG CCCTCAGTGC GGGTGGCCTG CTGTCAGCCC 
CCGGCTGCTG TACCCAACGG CTCCTATGCG CCACCTCGGG ACAAATTCGA AGAGTGCTCC 
AGAAAACTGA CCGCAGAACA GCTGGCCGCC ATCGACGATG AGGAGCTCCT GGATAAAATG 
CTGGATGAGT CCAAGGACTT TGAGGAGAGG AAGATGATCC GACAGGCCAT GAGAGATCTG 
CGTAAGAGGA AGAGAGAGGC CGAACTGGGA TGTACTCAAG AGGAAATAGA CCAGAGAGAA 
AAGGAGCGGG AGTTTCGTCT TGCGGAGCTT CGGCAGCAGA GAGCGGAGCA TACTCAGAAG 
GGTCGTACGG GAGGAGGAGC TGGAGAGGTG GTCATGAGGA AGGTGGAGAT GTCAGCTGAT 
GGCTCCACCC TCAGCCAAGT CACCAAGACA GACTGCTTTG CCCAGTCTGG TGATGGCAGC 
AGGACAGCTC ACAGCACCAT CATGGAGGCC AGTTATGTGA AGAAAACAGA CGTGGGGACA 
GTTCAGTCCA AGTCCTACAG CTGCTCCTCC TCTACCTCCA GCAGCAGCTC CACCAGGAAA 
GTGGGCAGTG TGTTTGACCG CGAGGACGAC TCGGTGTCCC GTGCTGCAGA GCGCCGGCAG 
GCAGAGAAGC GCAAGGAGCT GATGAGAGCC CAGACTCTGC CCAAGATGTC CGCCGCACAG 
TCCCGCAAAG CCATGATTGA GAAACTGGAC AAGGAGAGCG GAGGGCCAGG AAATAAAGCA 
GTTGCCAAGG TGAACACAGT CCAGCGCTCA GCTAGCTGCA TGGTGCCCAA TGCCAACTCC 
ATCAAACAGA TGCTCCTGGA CTGGTGCCGC GCCAAGACCC GCTCATACCA GAATGTGGAC 
ATCCAGAACT TCTCGTCCAG TTGGAGTGAT GGCATGGCGT TCTGTGCCCT GGTCCATAAC 
TTCTTCCCAG AGGCCTTTGA CTACTCCTCT CTCACCCCTG CCAACCGCAG ACACAACTTT 
GAGGTGGCCT TCAGCACTGC AGAGGAATGT GCCAACTGTA TGCCCCTGTT GGAGGTAGAG 
GACATGATGA TCATGGGGAG GAAGCCTGAC TCCAAGTGTG TGTTCACCTA TGTGCAGTCA 
CTGGTCAATC ACCTGCGCAG GCATGAGATG AAACTCGCCA AACTCCAGGG CACCAACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110536077 ( XM_021621609.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021477284.1 Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf56959
Clone ID Related Accession (Same CDS sequence)	XM_021621609.1
Accession Version	XM_021621609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	smoothelin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGGTCAGAG AGCAGAGTTG 
ACCCTGGGCC TCAGAGCGAC CCCCTTCAAG ATGTCCTCCT CCAGCCTTAC CTACGGATCC 
TCTTTCAAGA TGGCCTCAGA GCCCGTGTTG GCAGTGGAGC CCTCAGTGCG GGTGGCCTGC 
TGTCAGCCCC CGGCTGCTGT ACCCAACGGC TCCTATGCGC CACCTCGGGA CAAATTCGAA 
GAGTGCTCCA GAAAACTGAC CGCAGAACAG CTGGCCGCCA TCGACGATGA GGAGCTCCTG 
GATAAAATGC TGGATGAGTC CAAGGACTTT GAGGAGAGGA AGATGATCCG ACAGGCCATG 
AGAGATCTGC GTAAGAGGAA GAGAGAGGCC GAACTGGGAT GTACTCAAGA GGAAATAGAC 
CAGAGAGAAA AGGAGCGGGA GTTTCGTCTT GCGGAGCTTC GGCAGCAGAG AGCGGAGCAT 
ACTCAGAAGG GTCGTACGGG AGGAGGAGCT GGAGAGGTGG TCATGAGGAA GGTGGAGATG 
TCAGCTGATG GCTCCACCCT CAGCCAAGTC ACCAAGACAG ACTGCTTTGC CCAGTCTGGT 
GATGGCAGCA GGACAGCTCA CAGCACCATC ATGGAGGCCA GTTATGTGAA GAAAACAGAC 
GTGGGGACAG TTCAGTCCAA GTCCTACAGC TGCTCCTCCT CTACCTCCAG CAGCAGCTCC 
ACCAGGAAAG TGGGCAGTGT GTTTGACCGC GAGGACGACT CGGTGTCCCG TGCTGCAGAG 
CGCCGGCAGG CAGAGAAGCG CAAGGAGCTG ATGAGAGCCC AGACTCTGCC CAAGATGTCC 
GCCGCACAGT CCCGCAAAGC CATGATTGAG AAACTGGACA AGGAGAGCGG AGGGCCAGGA 
AATAAAGCAG TTGCCAAGGT GAACACAGTC CAGCGCTCAG CTAGCTGCAT GGTGCCCAAT 
GCCAACTCCA TCAAACAGAT GCTCCTGGAC TGGTGCCGCG CCAAGACCCG CTCATACCAG 
AATGTGGACA TCCAGAACTT CTCGTCCAGT TGGAGTGATG GCATGGCGTT CTGTGCCCTG 
GTCCATAACT TCTTCCCAGA GGCCTTTGAC TACTCCTCTC TCACCCCTGC CAACCGCAGA 
CACAACTTTG AGGTGGCCTT CAGCACTGCA GAGGAATGTG CCAACTGTAT GCCCCTGTTG 
GAGGTAGAGG ACATGATGAT CATGGGGAGG AAGCCTGACT CCAAGTGTGT GTTCACCTAT 
GTGCAGTCAC TGGTCAATCA CCTGCGCAGG CATGAGATGA AACTCGCCAA ACTCCAGGGC 
ACCAACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021477284.1
CDS	714..3905
Translation	MEKDPVSQRAMCRHGEECLISSHSDLICRLMDSVSMENEPILTQDTEKERLSERKRIKSQIRELRSHQTQQGRELHRTDSNSGMVLVLDPLVRDEVPGPLLTRPPTRDPVTSSEPDVVLSHRQRSDSTSSERSLASRPRLDSGASEKSLGLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASDRSQCSAHRGRLDSGASESHFRQRLDSGTSDSSMGPPAPLGSETSDGAGLLSRKRLDSGTSDLSVGVLSVGRSRVEQPEVTMSSSSSNTDSEAESTNHHPDQPHQDSTTTNHYQPDGAVLFRKAPDSHSGLLNGSAKERGDIQEQKVRYTLLTPLRKSNDAVPEKKEPVVLAPFGRANSVRDCMRKFTEPSPSVPVLKRGSQSLRNATAPSARVLGRATQLSEGPAAGTAKQTAVTTVVSITTPTDRPGRPHVGSTSYSSSASSSNNHVQCQGTVHPGGVATKPLQSAAQSLNAVGGAKQAAEKLRHASGGSKEDKTPGRASAQRDSKGAADPDMKTFLTIEIKDGRTTTTSSSSRGNLAPRISTNPIGQRAELTLGLRATPFKMSSSSLTYGSSFKMASEPVLAVEPSVRVACCQPPAAVPNGSYAPPRDKFEECSRKLTAEQLAAIDDEELLDKMLDESKDFEERKMIRQAMRDLRKRKREAELGCTQEEIDQREKEREFRLAELRQQRAEHTQKGRTGGGAGEVVMRKVEMSADGSTLSQVTKTDCFAQSGDGSRTAHSTIMEASYVKKTDVGTVQSKSYSCSSSTSSSSSTRKVGSVFDREDDSVSRAAERRQAEKRKELMRAQTLPKMSAAQSRKAMIEKLDKESGGPGNKAVAKVNTVQRSASCMVPNANSIKQMLLDWCRAKTRSYQNVDIQNFSSSWSDGMAFCALVHNFFPEAFDYSSLTPANRRHNFEVAFSTAEECANCMPLLEVEDMMIMGRKPDSKCVFTYVQSLVNHLRRHEMKLAKLQGTNL

Target ORF information:

RefSeq Version	XM_021621609.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621609.1

ORF Insert Sequence:

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGGTCAGAG AGCAGAGTTG 
ACCCTGGGCC TCAGAGCGAC CCCCTTCAAG ATGTCCTCCT CCAGCCTTAC CTACGGATCC 
TCTTTCAAGA TGGCCTCAGA GCCCGTGTTG GCAGTGGAGC CCTCAGTGCG GGTGGCCTGC 
TGTCAGCCCC CGGCTGCTGT ACCCAACGGC TCCTATGCGC CACCTCGGGA CAAATTCGAA 
GAGTGCTCCA GAAAACTGAC CGCAGAACAG CTGGCCGCCA TCGACGATGA GGAGCTCCTG 
GATAAAATGC TGGATGAGTC CAAGGACTTT GAGGAGAGGA AGATGATCCG ACAGGCCATG 
AGAGATCTGC GTAAGAGGAA GAGAGAGGCC GAACTGGGAT GTACTCAAGA GGAAATAGAC 
CAGAGAGAAA AGGAGCGGGA GTTTCGTCTT GCGGAGCTTC GGCAGCAGAG AGCGGAGCAT 
ACTCAGAAGG GTCGTACGGG AGGAGGAGCT GGAGAGGTGG TCATGAGGAA GGTGGAGATG 
TCAGCTGATG GCTCCACCCT CAGCCAAGTC ACCAAGACAG ACTGCTTTGC CCAGTCTGGT 
GATGGCAGCA GGACAGCTCA CAGCACCATC ATGGAGGCCA GTTATGTGAA GAAAACAGAC 
GTGGGGACAG TTCAGTCCAA GTCCTACAGC TGCTCCTCCT CTACCTCCAG CAGCAGCTCC 
ACCAGGAAAG TGGGCAGTGT GTTTGACCGC GAGGACGACT CGGTGTCCCG TGCTGCAGAG 
CGCCGGCAGG CAGAGAAGCG CAAGGAGCTG ATGAGAGCCC AGACTCTGCC CAAGATGTCC 
GCCGCACAGT CCCGCAAAGC CATGATTGAG AAACTGGACA AGGAGAGCGG AGGGCCAGGA 
AATAAAGCAG TTGCCAAGGT GAACACAGTC CAGCGCTCAG CTAGCTGCAT GGTGCCCAAT 
GCCAACTCCA TCAAACAGAT GCTCCTGGAC TGGTGCCGCG CCAAGACCCG CTCATACCAG 
AATGTGGACA TCCAGAACTT CTCGTCCAGT TGGAGTGATG GCATGGCGTT CTGTGCCCTG 
GTCCATAACT TCTTCCCAGA GGCCTTTGAC TACTCCTCTC TCACCCCTGC CAACCGCAGA 
CACAACTTTG AGGTGGCCTT CAGCACTGCA GAGGAATGTG CCAACTGTAT GCCCCTGTTG 
GAGGTAGAGG ACATGATGAT CATGGGGAGG AAGCCTGACT CCAAGTGTGT GTTCACCTAT 
GTGCAGTCAC TGGTCAATCA CCTGCGCAGG CATGAGATGA AACTCGCCAA ACTCCAGGGC 
ACCAACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110536077 ( XM_021621610.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021477285.1 Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf56960
Clone ID Related Accession (Same CDS sequence)	XM_021621610.1
Accession Version	XM_021621610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	smoothelin-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGGTCAGAG AGCAGAGTTG 
ACCCTGGGCC TCAGAGCGAC CCCCTTCAAG ATGTCCTCCT CCAGCCTTAC CTACGGATCC 
TCTTTCAAGA TGGCCTCAGA GCCCGTGTTG GCAGTGGAGC CCTCAGTGCG GGTGGCCTGC 
TGTCAGCCCC CGGCTGCTGT ACCCAACGGC TCCTATGCGC CACCTCGGGA CAAATTCGAA 
GAGTGCTCCA GAAAACTGAC CGCAGAACAG CTGGCCGCCA TCGACGATGA GGAGCTCCTG 
GATAAAATGC TGGATGAGTC CAAGGACTTT GAGGAGAGGA AGATGATCCG ACAGGCCATG 
AGAGATCTGC GTAAGAGGAA GAGAGAGGCC GAACTGGGAT GTACTCAAGA GGAAATAGAC 
CAGAGAGAAA AGGAGCGGGA GTTTCGTCTT GCGGAGCTTC GGCAGCAGAG AGCGGAGCAT 
ACTCAGAAGG GTCGTACGGG AGGAGGAGCT GGAGAGGTGG TCATGAGGAA GGTGGAGATG 
TCAGCTGATG GCTCCACCCT CAGCCAAGTC ACCAAGACAG ACTGCTTTGC CCAGTCTGGT 
GATGGCAGCA GGACAGCTCA CAGCACCATC ATGGAGGCCA GTTATGTGAA GAAAACAGAC 
GTGGGGACAG TTCAGTCCAA GTCCTACAGC TGCTCCTCCT CTACCTCCAG CAGCAGCTCC 
ACCAGGAAAG TGGGCAGTGT GTTTGACCGC GAGGACGACT CGGTGTCCCG TGCTGCAGAG 
CGCCGGCAGG CAGAGAAGCG CAAGGAGCTG ATGAGAGCCC AGACTCTGCC CAAGATGTCC 
GCCGCACAGT CCCGCAAAGC CATGATTGAG AAACTGGACA AGGAGAGCGG AGGGCCAGGA 
AATAAAGCAG TTGCCAAGGT GAACACAGTC CAGCGCTCAG CTAGCTGCAT GGTGCCCAAT 
GCCAACTCCA TCAAACAGAT GCTCCTGGAC TGGTGCCGCG CCAAGACCCG CTCATACCAG 
AATGTGGACA TCCAGAACTT CTCGTCCAGT TGGAGTGATG GCATGGCGTT CTGTGCCCTG 
GTCCATAACT TCTTCCCAGA GGCCTTTGAC TACTCCTCTC TCACCCCTGC CAACCGCAGA 
CACAACTTTG AGGTGGCCTT CAGCACTGCA GAGAAGCTGG CTGATTGTCC CGAGCTGTTG 
GACGTGGAGG ACATGGTGCG GATGCGTGAG CCGGATTGGA AGTGTGTGTA CACGTACCTG 
CAGGAGTTCT ACAGGGGCCT GGTGACCAAG GGCCTGGTTA AAACCAAAAA CTCTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021477285.1
CDS	717..3896
Translation	MEKDPVSQRAMCRHGEECLISSHSDLICRLMDSVSMENEPILTQDTEKERLSERKRIKSQIRELRSHQTQQGRELHRTDSNSGMVLVLDPLVRDEVPGPLLTRPPTRDPVTSSEPDVVLSHRQRSDSTSSERSLASRPRLDSGASEKSLGLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASDRSQCSAHRGRLDSGASESHFRQRLDSGTSDSSMGPPAPLGSETSDGAGLLSRKRLDSGTSDLSVGVLSVGRSRVEQPEVTMSSSSSNTDSEAESTNHHPDQPHQDSTTTNHYQPDGAVLFRKAPDSHSGLLNGSAKERGDIQEQKVRYTLLTPLRKSNDAVPEKKEPVVLAPFGRANSVRDCMRKFTEPSPSVPVLKRGSQSLRNATAPSARVLGRATQLSEGPAAGTAKQTAVTTVVSITTPTDRPGRPHVGSTSYSSSASSSNNHVQCQGTVHPGGVATKPLQSAAQSLNAVGGAKQAAEKLRHASGGSKEDKTPGRASAQRDSKGAADPDMKTFLTIEIKDGRTTTTSSSSRGNLAPRISTNPIGQRAELTLGLRATPFKMSSSSLTYGSSFKMASEPVLAVEPSVRVACCQPPAAVPNGSYAPPRDKFEECSRKLTAEQLAAIDDEELLDKMLDESKDFEERKMIRQAMRDLRKRKREAELGCTQEEIDQREKEREFRLAELRQQRAEHTQKGRTGGGAGEVVMRKVEMSADGSTLSQVTKTDCFAQSGDGSRTAHSTIMEASYVKKTDVGTVQSKSYSCSSSTSSSSSTRKVGSVFDREDDSVSRAAERRQAEKRKELMRAQTLPKMSAAQSRKAMIEKLDKESGGPGNKAVAKVNTVQRSASCMVPNANSIKQMLLDWCRAKTRSYQNVDIQNFSSSWSDGMAFCALVHNFFPEAFDYSSLTPANRRHNFEVAFSTAEKLADCPELLDVEDMVRMREPDWKCVYTYLQEFYRGLVTKGLVKTKNSS

Target ORF information:

RefSeq Version	XM_021621610.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621610.1

ORF Insert Sequence:

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGGTCAGAG AGCAGAGTTG 
ACCCTGGGCC TCAGAGCGAC CCCCTTCAAG ATGTCCTCCT CCAGCCTTAC CTACGGATCC 
TCTTTCAAGA TGGCCTCAGA GCCCGTGTTG GCAGTGGAGC CCTCAGTGCG GGTGGCCTGC 
TGTCAGCCCC CGGCTGCTGT ACCCAACGGC TCCTATGCGC CACCTCGGGA CAAATTCGAA 
GAGTGCTCCA GAAAACTGAC CGCAGAACAG CTGGCCGCCA TCGACGATGA GGAGCTCCTG 
GATAAAATGC TGGATGAGTC CAAGGACTTT GAGGAGAGGA AGATGATCCG ACAGGCCATG 
AGAGATCTGC GTAAGAGGAA GAGAGAGGCC GAACTGGGAT GTACTCAAGA GGAAATAGAC 
CAGAGAGAAA AGGAGCGGGA GTTTCGTCTT GCGGAGCTTC GGCAGCAGAG AGCGGAGCAT 
ACTCAGAAGG GTCGTACGGG AGGAGGAGCT GGAGAGGTGG TCATGAGGAA GGTGGAGATG 
TCAGCTGATG GCTCCACCCT CAGCCAAGTC ACCAAGACAG ACTGCTTTGC CCAGTCTGGT 
GATGGCAGCA GGACAGCTCA CAGCACCATC ATGGAGGCCA GTTATGTGAA GAAAACAGAC 
GTGGGGACAG TTCAGTCCAA GTCCTACAGC TGCTCCTCCT CTACCTCCAG CAGCAGCTCC 
ACCAGGAAAG TGGGCAGTGT GTTTGACCGC GAGGACGACT CGGTGTCCCG TGCTGCAGAG 
CGCCGGCAGG CAGAGAAGCG CAAGGAGCTG ATGAGAGCCC AGACTCTGCC CAAGATGTCC 
GCCGCACAGT CCCGCAAAGC CATGATTGAG AAACTGGACA AGGAGAGCGG AGGGCCAGGA 
AATAAAGCAG TTGCCAAGGT GAACACAGTC CAGCGCTCAG CTAGCTGCAT GGTGCCCAAT 
GCCAACTCCA TCAAACAGAT GCTCCTGGAC TGGTGCCGCG CCAAGACCCG CTCATACCAG 
AATGTGGACA TCCAGAACTT CTCGTCCAGT TGGAGTGATG GCATGGCGTT CTGTGCCCTG 
GTCCATAACT TCTTCCCAGA GGCCTTTGAC TACTCCTCTC TCACCCCTGC CAACCGCAGA 
CACAACTTTG AGGTGGCCTT CAGCACTGCA GAGAAGCTGG CTGATTGTCC CGAGCTGTTG 
GACGTGGAGG ACATGGTGCG GATGCGTGAG CCGGATTGGA AGTGTGTGTA CACGTACCTG 
CAGGAGTTCT ACAGGGGCCT GGTGACCAAG GGCCTGGTTA AAACCAAAAA CTCTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110536077 ( XM_021621611.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021477286.1 Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOf56961
Clone ID Related Accession (Same CDS sequence)	XM_021621611.1
Accession Version	XM_021621611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	smoothelin-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGAGTTGAC CCTGGGCCTC 
AGAGCGACCC CCTTCAAGAT GTCCTCCTCC AGCCTTACCT ACGGATCCTC TTTCAAGATG 
GCCTCAGAGC CCGTGTTGGC AGTGGAGCCC TCAGTGCGGG TGGCCTGCTG TCAGCCCCCG 
GCTGCTGTAC CCAACGGCTC CTATGCGCCA CCTCGGGACA AATTCGAAGA GTGCTCCAGA 
AAACTGACCG CAGAACAGCT GGCCGCCATC GACGATGAGG AGCTCCTGGA TAAAATGCTG 
GATGAGTCCA AGGACTTTGA GGAGAGGAAG ATGATCCGAC AGGCCATGAG AGATCTGCGT 
AAGAGGAAGA GAGAGGCCGA ACTGGGATGT ACTCAAGAGG AAATAGACCA GAGAGAAAAG 
GAGCGGGAGT TTCGTCTTGC GGAGCTTCGG CAGCAGAGAG CGGAGCATAC TCAGAAGGGT 
CGTACGGGAG GAGGAGCTGG AGAGGTGGTC ATGAGGAAGG TGGAGATGTC AGCTGATGGC 
TCCACCCTCA GCCAAGTCAC CAAGACAGAC TGCTTTGCCC AGTCTGGTGA TGGCAGCAGG 
ACAGCTCACA GCACCATCAT GGAGGCCAGT TATGTGAAGA AAACAGACGT GGGGACAGTT 
CAGTCCAAGT CCTACAGCTG CTCCTCCTCT ACCTCCAGCA GCAGCTCCAC CAGGAAAGTG 
GGCAGTGTGT TTGACCGCGA GGACGACTCG GTGTCCCGTG CTGCAGAGCG CCGGCAGGCA 
GAGAAGCGCA AGGAGCTGAT GAGAGCCCAG ACTCTGCCCA AGATGTCCGC CGCACAGTCC 
CGCAAAGCCA TGATTGAGAA ACTGGACAAG GAGAGCGGAG GGCCAGGAAA TAAAGCAGTT 
GCCAAGGTGA ACACAGTCCA GCGCTCAGCT AGCTGCATGG TGCCCAATGC CAACTCCATC 
AAACAGATGC TCCTGGACTG GTGCCGCGCC AAGACCCGCT CATACCAGAA TGTGGACATC 
CAGAACTTCT CGTCCAGTTG GAGTGATGGC ATGGCGTTCT GTGCCCTGGT CCATAACTTC 
TTCCCAGAGG CCTTTGACTA CTCCTCTCTC ACCCCTGCCA ACCGCAGACA CAACTTTGAG 
GTGGCCTTCA GCACTGCAGA GGAATGTGCC AACTGTATGC CCCTGTTGGA GGTAGAGGAC 
ATGATGATCA TGGGGAGGAA GCCTGACTCC AAGTGTGTGT TCACCTATGT GCAGTCACTG 
GTCAATCACC TGCGCAGGCA TGAGATGAAA CTCGCCAAAC TCCAGGGCAC CAACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021477286.1
CDS	717..3896
Translation	MEKDPVSQRAMCRHGEECLISSHSDLICRLMDSVSMENEPILTQDTEKERLSERKRIKSQIRELRSHQTQQGRELHRTDSNSGMVLVLDPLVRDEVPGPLLTRPPTRDPVTSSEPDVVLSHRQRSDSTSSERSLASRPRLDSGASEKSLGLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASDRSQCSAHRGRLDSGASESHFRQRLDSGTSDSSMGPPAPLGSETSDGAGLLSRKRLDSGTSDLSVGVLSVGRSRVEQPEVTMSSSSSNTDSEAESTNHHPDQPHQDSTTTNHYQPDGAVLFRKAPDSHSGLLNGSAKERGDIQEQKVRYTLLTPLRKSNDAVPEKKEPVVLAPFGRANSVRDCMRKFTEPSPSVPVLKRGSQSLRNATAPSARVLGRATQLSEGPAAGTAKQTAVTTVVSITTPTDRPGRPHVGSTSYSSSASSSNNHVQCQGTVHPGGVATKPLQSAAQSLNAVGGAKQAAEKLRHASGGSKEDKTPGRASAQRDSKGAADPDMKTFLTIEIKDGRTTTTSSSSRGNLAPRISTNPIELTLGLRATPFKMSSSSLTYGSSFKMASEPVLAVEPSVRVACCQPPAAVPNGSYAPPRDKFEECSRKLTAEQLAAIDDEELLDKMLDESKDFEERKMIRQAMRDLRKRKREAELGCTQEEIDQREKEREFRLAELRQQRAEHTQKGRTGGGAGEVVMRKVEMSADGSTLSQVTKTDCFAQSGDGSRTAHSTIMEASYVKKTDVGTVQSKSYSCSSSTSSSSSTRKVGSVFDREDDSVSRAAERRQAEKRKELMRAQTLPKMSAAQSRKAMIEKLDKESGGPGNKAVAKVNTVQRSASCMVPNANSIKQMLLDWCRAKTRSYQNVDIQNFSSSWSDGMAFCALVHNFFPEAFDYSSLTPANRRHNFEVAFSTAEECANCMPLLEVEDMMIMGRKPDSKCVFTYVQSLVNHLRRHEMKLAKLQGTNL

Target ORF information:

RefSeq Version	XM_021621611.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621611.1

ORF Insert Sequence:

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGAGTTGAC CCTGGGCCTC 
AGAGCGACCC CCTTCAAGAT GTCCTCCTCC AGCCTTACCT ACGGATCCTC TTTCAAGATG 
GCCTCAGAGC CCGTGTTGGC AGTGGAGCCC TCAGTGCGGG TGGCCTGCTG TCAGCCCCCG 
GCTGCTGTAC CCAACGGCTC CTATGCGCCA CCTCGGGACA AATTCGAAGA GTGCTCCAGA 
AAACTGACCG CAGAACAGCT GGCCGCCATC GACGATGAGG AGCTCCTGGA TAAAATGCTG 
GATGAGTCCA AGGACTTTGA GGAGAGGAAG ATGATCCGAC AGGCCATGAG AGATCTGCGT 
AAGAGGAAGA GAGAGGCCGA ACTGGGATGT ACTCAAGAGG AAATAGACCA GAGAGAAAAG 
GAGCGGGAGT TTCGTCTTGC GGAGCTTCGG CAGCAGAGAG CGGAGCATAC TCAGAAGGGT 
CGTACGGGAG GAGGAGCTGG AGAGGTGGTC ATGAGGAAGG TGGAGATGTC AGCTGATGGC 
TCCACCCTCA GCCAAGTCAC CAAGACAGAC TGCTTTGCCC AGTCTGGTGA TGGCAGCAGG 
ACAGCTCACA GCACCATCAT GGAGGCCAGT TATGTGAAGA AAACAGACGT GGGGACAGTT 
CAGTCCAAGT CCTACAGCTG CTCCTCCTCT ACCTCCAGCA GCAGCTCCAC CAGGAAAGTG 
GGCAGTGTGT TTGACCGCGA GGACGACTCG GTGTCCCGTG CTGCAGAGCG CCGGCAGGCA 
GAGAAGCGCA AGGAGCTGAT GAGAGCCCAG ACTCTGCCCA AGATGTCCGC CGCACAGTCC 
CGCAAAGCCA TGATTGAGAA ACTGGACAAG GAGAGCGGAG GGCCAGGAAA TAAAGCAGTT 
GCCAAGGTGA ACACAGTCCA GCGCTCAGCT AGCTGCATGG TGCCCAATGC CAACTCCATC 
AAACAGATGC TCCTGGACTG GTGCCGCGCC AAGACCCGCT CATACCAGAA TGTGGACATC 
CAGAACTTCT CGTCCAGTTG GAGTGATGGC ATGGCGTTCT GTGCCCTGGT CCATAACTTC 
TTCCCAGAGG CCTTTGACTA CTCCTCTCTC ACCCCTGCCA ACCGCAGACA CAACTTTGAG 
GTGGCCTTCA GCACTGCAGA GGAATGTGCC AACTGTATGC CCCTGTTGGA GGTAGAGGAC 
ATGATGATCA TGGGGAGGAA GCCTGACTCC AAGTGTGTGT TCACCTATGT GCAGTCACTG 
GTCAATCACC TGCGCAGGCA TGAGATGAAA CTCGCCAAAC TCCAGGGCAC CAACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110536077 ( XM_021621613.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021477288.1 Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOf56962
Clone ID Related Accession (Same CDS sequence)	XM_021621613.1
Accession Version	XM_021621613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	smoothelin-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGGTCAGAG AGCAGAGTTG 
ACCCTGGGCC TCAGAGCGAC CCCCTTCAAG ATGTCCTCCT CCAGCCTTAC CTACGGATCC 
TCTTTCAAGA TGGCCTCAGA GCCCGTGTTG GCAGTGGAGC CCTCAGTGCG GGTGGCCTGC 
TGTCAGCCCC CGGCTGCTGT ACCCAACGGC TCCTATGCGC CACCTCGGGA CAAATTCGAA 
GAGTGCTCCA GAAAACTGAC CGCAGAACAG CTGGCCGCCA TCGACGATGA GGAGCTCCTG 
GATAAAATGC TGGATGAGTC CAAGGACTTT GAGGAGAGGA AGATGATCCG ACAGGCCATG 
AGAGATCTGC GTAAGAGGAA GAGAGACCAG AGAGAAAAGG AGCGGGAGTT TCGTCTTGCG 
GAGCTTCGGC AGCAGAGAGC GGAGCATACT CAGAAGGGTC GTACGGGAGG AGGAGCTGGA 
GAGGTGGTCA TGAGGAAGGT GGAGATGTCA GCTGATGGCT CCACCCTCAG CCAAGTCACC 
AAGACAGACT GCTTTGCCCA GTCTGGTGAT GGCAGCAGGA CAGCTCACAG CACCATCATG 
GAGGCCAGTT ATGTGAAGAA AACAGACGTG GGGACAGTTC AGTCCAAGTC CTACAGCTGC 
TCCTCCTCTA CCTCCAGCAG CAGCTCCACC AGGAAAGTGG GCAGTGTGTT TGACCGCGAG 
GACGACTCGG TGTCCCGTGC TGCAGAGCGC CGGCAGGCAG AGAAGCGCAA GGAGCTGATG 
AGAGCCCAGA CTCTGCCCAA GATGTCCGCC GCACAGTCCC GCAAAGCCAT GATTGAGAAA 
CTGGACAAGG AGAGCGGAGG GCCAGGAAAT AAAGCAGTTG CCAAGGTGAA CACAGTCCAG 
CGCTCAGCTA GCTGCATGGT GCCCAATGCC AACTCCATCA AACAGATGCT CCTGGACTGG 
TGCCGCGCCA AGACCCGCTC ATACCAGAAT GTGGACATCC AGAACTTCTC GTCCAGTTGG 
AGTGATGGCA TGGCGTTCTG TGCCCTGGTC CATAACTTCT TCCCAGAGGC CTTTGACTAC 
TCCTCTCTCA CCCCTGCCAA CCGCAGACAC AACTTTGAGG TGGCCTTCAG CACTGCAGAG 
GAATGTGCCA ACTGTATGCC CCTGTTGGAG GTAGAGGACA TGATGATCAT GGGGAGGAAG 
CCTGACTCCA AGTGTGTGTT CACCTATGTG CAGTCACTGG TCAATCACCT GCGCAGGCAT 
GAGATGAAAC TCGCCAAACT CCAGGGCACC AACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021477288.1
CDS	715..3873
Translation	MEKDPVSQRAMCRHGEECLISSHSDLICRLMDSVSMENEPILTQDTEKERLSERKRIKSQIRELRSHQTQQGRELHRTDSNSGMVLVLDPLVRDEVPGPLLTRPPTRDPVTSSEPDVVLSHRQRSDSTSSERSLASRPRLDSGASEKSLGLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASDRSQCSAHRGRLDSGASESHFRQRLDSGTSDSSMGPPAPLGSETSDGAGLLSRKRLDSGTSDLSVGVLSVGRSRVEQPEVTMSSSSSNTDSEAESTNHHPDQPHQDSTTTNHYQPDGAVLFRKAPDSHSGLLNGSAKERGDIQEQKVRYTLLTPLRKSNDAVPEKKEPVVLAPFGRANSVRDCMRKFTEPSPSVPVLKRGSQSLRNATAPSARVLGRATQLSEGPAAGTAKQTAVTTVVSITTPTDRPGRPHVGSTSYSSSASSSNNHVQCQGTVHPGGVATKPLQSAAQSLNAVGGAKQAAEKLRHASGGSKEDKTPGRASAQRDSKGAADPDMKTFLTIEIKDGRTTTTSSSSRGNLAPRISTNPIGQRAELTLGLRATPFKMSSSSLTYGSSFKMASEPVLAVEPSVRVACCQPPAAVPNGSYAPPRDKFEECSRKLTAEQLAAIDDEELLDKMLDESKDFEERKMIRQAMRDLRKRKRDQREKEREFRLAELRQQRAEHTQKGRTGGGAGEVVMRKVEMSADGSTLSQVTKTDCFAQSGDGSRTAHSTIMEASYVKKTDVGTVQSKSYSCSSSTSSSSSTRKVGSVFDREDDSVSRAAERRQAEKRKELMRAQTLPKMSAAQSRKAMIEKLDKESGGPGNKAVAKVNTVQRSASCMVPNANSIKQMLLDWCRAKTRSYQNVDIQNFSSSWSDGMAFCALVHNFFPEAFDYSSLTPANRRHNFEVAFSTAEECANCMPLLEVEDMMIMGRKPDSKCVFTYVQSLVNHLRRHEMKLAKLQGTNL

Target ORF information:

RefSeq Version	XM_021621613.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621613.1

ORF Insert Sequence:

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGGTCAGAG AGCAGAGTTG 
ACCCTGGGCC TCAGAGCGAC CCCCTTCAAG ATGTCCTCCT CCAGCCTTAC CTACGGATCC 
TCTTTCAAGA TGGCCTCAGA GCCCGTGTTG GCAGTGGAGC CCTCAGTGCG GGTGGCCTGC 
TGTCAGCCCC CGGCTGCTGT ACCCAACGGC TCCTATGCGC CACCTCGGGA CAAATTCGAA 
GAGTGCTCCA GAAAACTGAC CGCAGAACAG CTGGCCGCCA TCGACGATGA GGAGCTCCTG 
GATAAAATGC TGGATGAGTC CAAGGACTTT GAGGAGAGGA AGATGATCCG ACAGGCCATG 
AGAGATCTGC GTAAGAGGAA GAGAGACCAG AGAGAAAAGG AGCGGGAGTT TCGTCTTGCG 
GAGCTTCGGC AGCAGAGAGC GGAGCATACT CAGAAGGGTC GTACGGGAGG AGGAGCTGGA 
GAGGTGGTCA TGAGGAAGGT GGAGATGTCA GCTGATGGCT CCACCCTCAG CCAAGTCACC 
AAGACAGACT GCTTTGCCCA GTCTGGTGAT GGCAGCAGGA CAGCTCACAG CACCATCATG 
GAGGCCAGTT ATGTGAAGAA AACAGACGTG GGGACAGTTC AGTCCAAGTC CTACAGCTGC 
TCCTCCTCTA CCTCCAGCAG CAGCTCCACC AGGAAAGTGG GCAGTGTGTT TGACCGCGAG 
GACGACTCGG TGTCCCGTGC TGCAGAGCGC CGGCAGGCAG AGAAGCGCAA GGAGCTGATG 
AGAGCCCAGA CTCTGCCCAA GATGTCCGCC GCACAGTCCC GCAAAGCCAT GATTGAGAAA 
CTGGACAAGG AGAGCGGAGG GCCAGGAAAT AAAGCAGTTG CCAAGGTGAA CACAGTCCAG 
CGCTCAGCTA GCTGCATGGT GCCCAATGCC AACTCCATCA AACAGATGCT CCTGGACTGG 
TGCCGCGCCA AGACCCGCTC ATACCAGAAT GTGGACATCC AGAACTTCTC GTCCAGTTGG 
AGTGATGGCA TGGCGTTCTG TGCCCTGGTC CATAACTTCT TCCCAGAGGC CTTTGACTAC 
TCCTCTCTCA CCCCTGCCAA CCGCAGACAC AACTTTGAGG TGGCCTTCAG CACTGCAGAG 
GAATGTGCCA ACTGTATGCC CCTGTTGGAG GTAGAGGACA TGATGATCAT GGGGAGGAAG 
CCTGACTCCA AGTGTGTGTT CACCTATGTG CAGTCACTGG TCAATCACCT GCGCAGGCAT 
GAGATGAAAC TCGCCAAACT CCAGGGCACC AACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110536077 ( XM_021621614.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021477289.1 Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOf56963
Clone ID Related Accession (Same CDS sequence)	XM_021621614.1
Accession Version	XM_021621614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	smoothelin-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGGTCAGAG AGCAGAGTTG 
ACCCTGGGCC TCAGAGCGAC CCCCTTCAAG ATGTCCTCCT CCAGCCTTAC CTACGGATCC 
TCTTTCAAGA TGGCCTCAGA GCCCGTGTTG GCAGTGGAGC CCTCAGTGCG GGTGGCCTGC 
TGTCAGCCCC CGGCTGCTGT ACCCAACGGC TCCTATGCGC CACCTCGGGA CAAATTCGAA 
GAGTGCTCCA GAAAACTGAC CGCAGAACAG CTGGCCGCCA TCGACGATGA GGAGCTCCTG 
GATAAAATGC TGGATGAGTC CAAGGACTTT GAGGAGAGGA AGATGATCCG ACAGGCCATG 
AGAGATCTGC GTAAGAGGAA GAGAGAGGCC GAACTGGGAT GTACTCAAGA GGAAATAGAC 
CAGAGAGAAA AGGAGCGGGA GTTTCGTCTT GCGGAGCTTC GGCAGCAGAG AGCGGAGCAT 
ACTCAGAAGG GTCGTACGGG AGGAGGAGCT GGAGAGGTGG TCATGAGGAA GGTGGAGATG 
TCAGCTGATG GCTCCACCCT CAGCCAAGTC ACCAAGACAG ACTGCTTTGC CCAGTCTGGT 
GATGGCAGCA GGACAGCTCA CAGCACCATC ATGGAGGCCA GTTATGTGAA GAAAACAGAC 
GTGGGGACAG TTCAGTCCAA GTCCTACAGC TGCTCCTCCT CTACCTCCAG CAGCAGCTCC 
ACCAGGAAAG TGGGCAGTGT GTTTGACCGC GAGGACGACT CGGTGTCCCG TGCTGCAGAG 
CGCCGGCAGG CAGAGAAGCG CAAGGAGCTG ATGAGAGCCC AGACTCTGCC CAAGATGTCC 
GCCGCACAGT CCCGCAAAGC CATGATTGAG AAACTGGACA AGGAGAGCGG AGGGCCAGGA 
AATAAAGCAG TTGCCAAGGT GAACACAGTC CAGCGCTCAG CTAGCTGCAT GGTGCCCAAT 
GCCAACTCCA TCAAACAGAT GCTCCTGGAC TGGTGCCGCG CCAAGACCCG CTCATACCAG 
AATGTGGACA TCCAGAACTT CTCGTCCAGT TGGAGTGATG GCATGGCGTT CTGTGCCCTG 
GTCCATAACT TCTTCCCAGA GGCCTTTGAC TACTCCTCTC TCACCCCTGC CAACCGCAGA 
CACAACTTTG AGGTGGCCTT CAGCACTGCA GAAGATGCTC TCCTCAGTAA CCGCTGTGTG 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021477289.1
CDS	718..3783
Translation	MEKDPVSQRAMCRHGEECLISSHSDLICRLMDSVSMENEPILTQDTEKERLSERKRIKSQIRELRSHQTQQGRELHRTDSNSGMVLVLDPLVRDEVPGPLLTRPPTRDPVTSSEPDVVLSHRQRSDSTSSERSLASRPRLDSGASEKSLGLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASDRSQCSAHRGRLDSGASESHFRQRLDSGTSDSSMGPPAPLGSETSDGAGLLSRKRLDSGTSDLSVGVLSVGRSRVEQPEVTMSSSSSNTDSEAESTNHHPDQPHQDSTTTNHYQPDGAVLFRKAPDSHSGLLNGSAKERGDIQEQKVRYTLLTPLRKSNDAVPEKKEPVVLAPFGRANSVRDCMRKFTEPSPSVPVLKRGSQSLRNATAPSARVLGRATQLSEGPAAGTAKQTAVTTVVSITTPTDRPGRPHVGSTSYSSSASSSNNHVQCQGTVHPGGVATKPLQSAAQSLNAVGGAKQAAEKLRHASGGSKEDKTPGRASAQRDSKGAADPDMKTFLTIEIKDGRTTTTSSSSRGNLAPRISTNPIGQRAELTLGLRATPFKMSSSSLTYGSSFKMASEPVLAVEPSVRVACCQPPAAVPNGSYAPPRDKFEECSRKLTAEQLAAIDDEELLDKMLDESKDFEERKMIRQAMRDLRKRKREAELGCTQEEIDQREKEREFRLAELRQQRAEHTQKGRTGGGAGEVVMRKVEMSADGSTLSQVTKTDCFAQSGDGSRTAHSTIMEASYVKKTDVGTVQSKSYSCSSSTSSSSSTRKVGSVFDREDDSVSRAAERRQAEKRKELMRAQTLPKMSAAQSRKAMIEKLDKESGGPGNKAVAKVNTVQRSASCMVPNANSIKQMLLDWCRAKTRSYQNVDIQNFSSSWSDGMAFCALVHNFFPEAFDYSSLTPANRRHNFEVAFSTAEDALLSNRCVT

Target ORF information:

RefSeq Version	XM_021621614.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621614.1

ORF Insert Sequence:

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGGTCAGAG AGCAGAGTTG 
ACCCTGGGCC TCAGAGCGAC CCCCTTCAAG ATGTCCTCCT CCAGCCTTAC CTACGGATCC 
TCTTTCAAGA TGGCCTCAGA GCCCGTGTTG GCAGTGGAGC CCTCAGTGCG GGTGGCCTGC 
TGTCAGCCCC CGGCTGCTGT ACCCAACGGC TCCTATGCGC CACCTCGGGA CAAATTCGAA 
GAGTGCTCCA GAAAACTGAC CGCAGAACAG CTGGCCGCCA TCGACGATGA GGAGCTCCTG 
GATAAAATGC TGGATGAGTC CAAGGACTTT GAGGAGAGGA AGATGATCCG ACAGGCCATG 
AGAGATCTGC GTAAGAGGAA GAGAGAGGCC GAACTGGGAT GTACTCAAGA GGAAATAGAC 
CAGAGAGAAA AGGAGCGGGA GTTTCGTCTT GCGGAGCTTC GGCAGCAGAG AGCGGAGCAT 
ACTCAGAAGG GTCGTACGGG AGGAGGAGCT GGAGAGGTGG TCATGAGGAA GGTGGAGATG 
TCAGCTGATG GCTCCACCCT CAGCCAAGTC ACCAAGACAG ACTGCTTTGC CCAGTCTGGT 
GATGGCAGCA GGACAGCTCA CAGCACCATC ATGGAGGCCA GTTATGTGAA GAAAACAGAC 
GTGGGGACAG TTCAGTCCAA GTCCTACAGC TGCTCCTCCT CTACCTCCAG CAGCAGCTCC 
ACCAGGAAAG TGGGCAGTGT GTTTGACCGC GAGGACGACT CGGTGTCCCG TGCTGCAGAG 
CGCCGGCAGG CAGAGAAGCG CAAGGAGCTG ATGAGAGCCC AGACTCTGCC CAAGATGTCC 
GCCGCACAGT CCCGCAAAGC CATGATTGAG AAACTGGACA AGGAGAGCGG AGGGCCAGGA 
AATAAAGCAG TTGCCAAGGT GAACACAGTC CAGCGCTCAG CTAGCTGCAT GGTGCCCAAT 
GCCAACTCCA TCAAACAGAT GCTCCTGGAC TGGTGCCGCG CCAAGACCCG CTCATACCAG 
AATGTGGACA TCCAGAACTT CTCGTCCAGT TGGAGTGATG GCATGGCGTT CTGTGCCCTG 
GTCCATAACT TCTTCCCAGA GGCCTTTGAC TACTCCTCTC TCACCCCTGC CAACCGCAGA 
CACAACTTTG AGGTGGCCTT CAGCACTGCA GAAGATGCTC TCCTCAGTAA CCGCTGTGTG 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110536077 ( XM_021621616.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021477291.1 Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X7, mRNA.

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CloneID	OOf56965
Clone ID Related Accession (Same CDS sequence)	XM_021621616.1
Accession Version	XM_021621616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	smoothelin-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGGTCAGAG AGCAGAGTTG 
ACCCTGGGCC TCAGAGCGAC CCCCTTCAAG ATGTCCTCCT CCAGCCTTAC CTACGGATCC 
TCTTTCAAGA TGGCCTCAGA GCCCGTGTTG GCAGTGGAGC CCTCAGTGCG GGTGGCCTGC 
TGTCAGCCCC CGGCTGCTGT ACCCAACGGC TCCTATGCGC CACCTCGGGA CAAATTCGAA 
GAGTGCTCCA GAAAACTGAC CGCAGAACAG CTGGCCGCCA TCGACGATGA GGAGCTCCTG 
GATAAAATGC TGGATGAGTC CAAGGACTTT GAGGAGAGGA AGATGATCCG ACAGGCCATG 
AGAGATCTGC GTAAGAGGAA GAGAGACCAG AGAGAAAAGG AGCGGGAGTT TCGTCTTGCG 
GAGCTTCGGC AGCAGAGAGC GGAGCATACT CAGAAGGGTC GTACGGGAGG AGGAGCTGGA 
GAGGTGGTCA TGAGGAAGGT GGAGATGTCA GCTGATGGCT CCACCCTCAG CCAAGTCACC 
AAGACAGACT GCTTTGCCCA GTCTGGTGAT GGCAGCAGGA CAGCTCACAG CACCATCATG 
GAGGCCAGTT ATGTGAAGAA AACAGACGTG GGGACAGTTC AGTCCAAGTC CTACAGCTGC 
TCCTCCTCTA CCTCCAGCAG CAGCTCCACC AGGAAAGTGG GCAGTGTGTT TGACCGCGAG 
GACGACTCGG TGTCCCGTGC TGCAGAGCGC CGGCAGGCAG AGAAGCGCAA GGAGCTGATG 
AGAGCCCAGA CTCTGCCCAA GATGTCCGCC GCACAGTCCC GCAAAGCCAT GATTGAGAAA 
CTGGACAAGG AGAGCGGAGG GCCAGGAAAT AAAGCAGTTG CCAAGGTGAA CACAGTCCAG 
CGCTCAGCTA GCTGCATGGT GCCCAATGCC AACTCCATCA AACAGATGCT CCTGGACTGG 
TGCCGCGCCA AGACCCGCTC ATACCAGAAT GTGGACATCC AGAACTTCTC GTCCAGTTGG 
AGTGATGGCA TGGCGTTCTG TGCCCTGGTC CATAACTTCT TCCCAGAGGC CTTTGACTAC 
TCCTCTCTCA CCCCTGCCAA CCGCAGACAC AACTTTGAGG TGGCCTTCAG CACTGCAGAG 
AAGCTGGCTG ATTGTCCCGA GCTGTTGGAC GTGGAGGACA TGGTGCGGAT GCGTGAGCCG 
GATTGGAAGT GTGTGTACAC GTACCTGCAG GAGTTCTACA GGGGCCTGGT GACCAAGGGC 
CTGGTTAAAA CCAAAAACTC TTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021477291.1
CDS	718..3864
Translation	MEKDPVSQRAMCRHGEECLISSHSDLICRLMDSVSMENEPILTQDTEKERLSERKRIKSQIRELRSHQTQQGRELHRTDSNSGMVLVLDPLVRDEVPGPLLTRPPTRDPVTSSEPDVVLSHRQRSDSTSSERSLASRPRLDSGASEKSLGLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASEKSLGSAFRTRVDSGASDRSQCSAHRGRLDSGASESHFRQRLDSGTSDSSMGPPAPLGSETSDGAGLLSRKRLDSGTSDLSVGVLSVGRSRVEQPEVTMSSSSSNTDSEAESTNHHPDQPHQDSTTTNHYQPDGAVLFRKAPDSHSGLLNGSAKERGDIQEQKVRYTLLTPLRKSNDAVPEKKEPVVLAPFGRANSVRDCMRKFTEPSPSVPVLKRGSQSLRNATAPSARVLGRATQLSEGPAAGTAKQTAVTTVVSITTPTDRPGRPHVGSTSYSSSASSSNNHVQCQGTVHPGGVATKPLQSAAQSLNAVGGAKQAAEKLRHASGGSKEDKTPGRASAQRDSKGAADPDMKTFLTIEIKDGRTTTTSSSSRGNLAPRISTNPIGQRAELTLGLRATPFKMSSSSLTYGSSFKMASEPVLAVEPSVRVACCQPPAAVPNGSYAPPRDKFEECSRKLTAEQLAAIDDEELLDKMLDESKDFEERKMIRQAMRDLRKRKRDQREKEREFRLAELRQQRAEHTQKGRTGGGAGEVVMRKVEMSADGSTLSQVTKTDCFAQSGDGSRTAHSTIMEASYVKKTDVGTVQSKSYSCSSSTSSSSSTRKVGSVFDREDDSVSRAAERRQAEKRKELMRAQTLPKMSAAQSRKAMIEKLDKESGGPGNKAVAKVNTVQRSASCMVPNANSIKQMLLDWCRAKTRSYQNVDIQNFSSSWSDGMAFCALVHNFFPEAFDYSSLTPANRRHNFEVAFSTAEKLADCPELLDVEDMVRMREPDWKCVYTYLQEFYRGLVTKGLVKTKNSS

Target ORF information:

RefSeq Version	XM_021621616.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss smoothelin-like (LOC110536077), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621616.1

ORF Insert Sequence:

ATGGAAAAGG ACCCTGTCTC TCAGCGAGCC ATGTGCAGGC ATGGAGAGGA GTGCCTGATC 
TCATCACATT CTGATCTCAT CTGCAGACTG ATGGATTCAG TTTCCATGGA AAATGAGCCA 
ATTCTAACCC AGGACACAGA GAAGGAGAGG CTTAGCGAGC GAAAGAGAAT CAAGTCTCAG 
ATCCGGGAGC TACGGAGCCA CCAGACGCAG CAGGGCAGAG AGCTGCACAG GACAGACTCC 
AACTCAGGCA TGGTTCTGGT TCTGGACCCC CTGGTGAGGG ATGAGGTCCC GGGCCCTCTG 
CTGACCCGCC CACCGACCCG GGACCCTGTG ACATCATCAG AGCCGGACGT GGTCCTATCC 
CACCGCCAGC GTTCTGACTC GACATCCTCA GAACGCAGCC TGGCCTCCAG GCCCCGTCTG 
GACTCTGGAG CCTCAGAGAA GAGTCTGGGT CTGGGCTCAG CCTTTCGGAC CAGGGTGGAC 
TCAGGAGCAT CAGAAAAGAG CTTGGGCTCA GCCTTCAGGA CCAGGGTGGA CTCTGGAGCA 
TCAGAAAAGA GCCTGGGCTC AGCCTTCAGG ACCAGGGTGG ACTCTGGAGC ATCAGAAAAG 
AGCCTGGGCT CAGCCTTCCG GACCAGGGTG GACTCAGGAG CATCAGAAAA GAGCCTGGGC 
TCAGCCTTCC GGACCAGGGT GGACTCTGGA GCATCAGAAA AGAGCCTGGG CTCAGCCTTC 
AGGACCAGGG TGGACTCTGG AGCATCAGAA AAGAGCCTGG GCTCAGCCTT CAGGACCAGG 
GTGGACTCAG GAGCATCAGA CAGAAGCCAG TGTTCCGCCC ACAGAGGCAG ACTGGACTCT 
GGGGCCTCAG AGTCCCACTT CAGACAGAGA CTGGACTCTG GCACATCAGA CTCCAGTATG 
GGACCGCCAG CCCCCCTGGG CTCTGAGACC TCCGACGGCG CAGGGCTCCT GTCACGGAAA 
CGGTTGGACT CAGGGACGTC TGACCTGAGT GTGGGCGTGT TGTCTGTGGG CCGGAGCCGT 
GTAGAGCAGC CAGAGGTGAC CATGAGTAGC TCCTCCTCCA ACACAGACTC TGAGGCTGAA 
AGCACCAACC ACCATCCAGA CCAGCCCCAT CAGGACAGCA CCACTACCAA CCATTATCAG 
CCTGATGGGG CCGTCCTATT CAGGAAAGCC CCAGACTCCC ACAGTGGCCT GCTCAATGGC 
AGTGCCAAGG AAAGAGGAGA CATCCAGGAA CAGAAGGTCA GATATACTTT ACTCACTCCT 
CTCAGAAAAT CAAATGATGC AGTTCCTGAG AAGAAAGAGC CGGTTGTCTT GGCCCCGTTC 
GGCCGGGCTA ACTCTGTGCG GGACTGTATG CGTAAGTTCA CGGAGCCCAG TCCCAGTGTC 
CCGGTCCTGA AGAGGGGCTC TCAGTCTCTG AGGAACGCGA CAGCCCCCAG TGCCCGAGTC 
CTAGGCAGAG CCACCCAGCT GAGTGAGGGG CCAGCAGCAG GGACAGCCAA GCAGACAGCA 
GTGACCACAG TAGTATCCAT CACCACCCCT ACAGACAGAC CCGGGAGACC CCATGTAGGC 
TCTACATCCT ACTCTTCATC CGCCTCCTCC TCAAATAACC ATGTCCAATG CCAGGGCACA 
GTTCATCCAG GGGGCGTGGC CACCAAACCT CTGCAAAGCG CTGCTCAATC CTTGAACGCT 
GTAGGCGGGG CAAAGCAGGC GGCTGAAAAG CTGCGGCACG CCTCAGGCGG CTCAAAGGAA 
GACAAGACCC CAGGGAGAGC ATCTGCACAG CGGGACAGCA AGGGAGCGGC AGACCCCGAC 
ATGAAGACCT TCCTAACTAT CGAGATCAAA GATGGGCGCA CCACCACCAC CTCTTCATCC 
TCTAGAGGCA ACCTGGCCCC ACGCATCTCC ACCAACCCCA TAGGTCAGAG AGCAGAGTTG 
ACCCTGGGCC TCAGAGCGAC CCCCTTCAAG ATGTCCTCCT CCAGCCTTAC CTACGGATCC 
TCTTTCAAGA TGGCCTCAGA GCCCGTGTTG GCAGTGGAGC CCTCAGTGCG GGTGGCCTGC 
TGTCAGCCCC CGGCTGCTGT ACCCAACGGC TCCTATGCGC CACCTCGGGA CAAATTCGAA 
GAGTGCTCCA GAAAACTGAC CGCAGAACAG CTGGCCGCCA TCGACGATGA GGAGCTCCTG 
GATAAAATGC TGGATGAGTC CAAGGACTTT GAGGAGAGGA AGATGATCCG ACAGGCCATG 
AGAGATCTGC GTAAGAGGAA GAGAGACCAG AGAGAAAAGG AGCGGGAGTT TCGTCTTGCG 
GAGCTTCGGC AGCAGAGAGC GGAGCATACT CAGAAGGGTC GTACGGGAGG AGGAGCTGGA 
GAGGTGGTCA TGAGGAAGGT GGAGATGTCA GCTGATGGCT CCACCCTCAG CCAAGTCACC 
AAGACAGACT GCTTTGCCCA GTCTGGTGAT GGCAGCAGGA CAGCTCACAG CACCATCATG 
GAGGCCAGTT ATGTGAAGAA AACAGACGTG GGGACAGTTC AGTCCAAGTC CTACAGCTGC 
TCCTCCTCTA CCTCCAGCAG CAGCTCCACC AGGAAAGTGG GCAGTGTGTT TGACCGCGAG 
GACGACTCGG TGTCCCGTGC TGCAGAGCGC CGGCAGGCAG AGAAGCGCAA GGAGCTGATG 
AGAGCCCAGA CTCTGCCCAA GATGTCCGCC GCACAGTCCC GCAAAGCCAT GATTGAGAAA 
CTGGACAAGG AGAGCGGAGG GCCAGGAAAT AAAGCAGTTG CCAAGGTGAA CACAGTCCAG 
CGCTCAGCTA GCTGCATGGT GCCCAATGCC AACTCCATCA AACAGATGCT CCTGGACTGG 
TGCCGCGCCA AGACCCGCTC ATACCAGAAT GTGGACATCC AGAACTTCTC GTCCAGTTGG 
AGTGATGGCA TGGCGTTCTG TGCCCTGGTC CATAACTTCT TCCCAGAGGC CTTTGACTAC 
TCCTCTCTCA CCCCTGCCAA CCGCAGACAC AACTTTGAGG TGGCCTTCAG CACTGCAGAG 
AAGCTGGCTG ATTGTCCCGA GCTGTTGGAC GTGGAGGACA TGGTGCGGAT GCGTGAGCCG 
GATTGGAAGT GTGTGTACAC GTACCTGCAG GAGTTCTACA GGGGCCTGGT GACCAAGGGC 
CTGGTTAAAA CCAAAAACTC TTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC110536077 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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