vps13a cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	vps13a
Entrez Gene ID	110535782
Full Name	vacuolar protein sorting 13 homolog A
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021621035.1	XP_021476710.1	vacuolar protein sorting-associated protein 13A isoform X1
XM_021621038.1	XP_021476713.1	vacuolar protein sorting-associated protein 13A isoform X4
XM_021621036.1	XP_021476711.1	vacuolar protein sorting-associated protein 13A isoform X2
XM_021621041.1	XP_021476716.1	vacuolar protein sorting-associated protein 13A isoform X7
XM_021621037.1	XP_021476712.1	vacuolar protein sorting-associated protein 13A isoform X3
XM_021621040.1	XP_021476715.1	vacuolar protein sorting-associated protein 13A isoform X6
XM_021621039.1	XP_021476714.1	vacuolar protein sorting-associated protein 13A isoform X5

Pathways from BioSystems
Gene Ontology

The following vps13a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps13a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOf56503	XM_021621035.1 Latest version!	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OOf56504	XM_021621036.1 Latest version!	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OOf56505	XM_021621037.1 Latest version!	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OOf56506	XM_021621038.1 Latest version!	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OOf56507	XM_021621039.1 Latest version!	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OOf56508	XM_021621040.1 Latest version!	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OOf56509	XM_021621041.1 Latest version!	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vps13a ( XM_021621035.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021476710.1 Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf56503
Clone ID Related Accession (Same CDS sequence)	XM_021621035.1
Accession Version	XM_021621035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	vacuolar protein sorting-associated protein 13A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGT 
ACTGGTACTA ACCCCACCAG CGGCAACACG GTGGTAACTG CTGCAGTGGT GGAGACCCAG 
AAGAGCACCA AGCTGAAGAG CACACTCAAA CTGGACTTCA AGTTTGACTC CATGACCCTG 
GTCCTGTACA GCCCCAATGG GACTGAGGTG CAGGTGTTGG ACTCGAGGGA TGAGCAGCTG 
AAGCTGGCTG AGTTTACCCT GGGGACTATC TCTACATCTG TCCACATGTT CTCAGACGGA 
TCTATGAAGG CCTCCGTCAA ACTCACAGAC TGCCTGCTAG ACGACAAAAG ATGCAGGGTC 
AAGAAGATCA CTGCCAGGAT GATGGCGATG CGTCCGGGCG CTGAGAGGAA CGTGATGGTG 
GAGGTGAACT ACAAGCAGGG CCGCGACGGC ACCTCTTTGG ACACGGTGGT GCAGGACGTG 
TACCTCTGTG CCAGCATGGA GTTCCTGCTC ACTGTGGCCG ACATCTTCCT CAAAGCCAGC 
CAGCAGGGCT TCTCCTCCGC CACCCCCAAG AACAGCAGTA GCACTAAACC CACTGCAGCC 
GCAGCCTCCA AGGAATCTGC TCCAGTGGGG GTGGTGGCAA AGTCAGAGAT TAATGTTCTG 
GTGAAGAACC CCGAGATTGT GTTTGTGGCC GACCTGACAC GTGCCGACGC CCCGGCCCTG 
GTCATGACCA CGGAGTGTGA GCTGGTGATG AAGAGCGACC TGGACGGTCA GCTGATGACT 
GCGGTGGTCA AGGACCTGAA GGTGGTGGCC TGCCCCTTCC TGAAGGAGAA GAGAAACAAG 
GAGACCACTG TGCTGCAGCC CTGCCAAGTG TTCTTCAAGA GCACACAGTC CTCCACCTCC 
CCGCAGGCCA TGGAGGTCTG CATCAACGCA CTCACACTCA AGGTGTCGCC CATCATTATC 
AACACAGTGA TTACCATTCA GTCGGTGCTG AGCCCGGCCG GTGCCGAGAC ACCCCAGTCT 
GAGCTAGAGA GCCCCGTGCC CCCAGACCTG TGGGAGCGCC GGTCCTGGAG GGACCTCAAG 
CTGTGGTTCC TGGAGGAGGA GGGTAAAGAC GAGGATGCCC AGTCCCTGGT CCCGCTGATT 
CCCCAGGGCG AGTCCCTGCA GTTGGCCATC AACTCCATCT GCCTGACACT GGAGGCAGGG 
GTGGGCCACC GCACCGTCCC CATGCTCCTG GCCAAGTCCT CCTTCCACGG TGACGTCAAG 
AACTGGAGCA CCCTCATCAA CCTGCACAGC CAGCTCAACC TGGAGGTTCA CTACTTCAAC 
GAGGTGATGG GGGTGTGGGA GCCACTGCTA GAGCCCCTAG AAGACGAGAC CCGAGATGGA 
TTCAGACCCT GGACCCTGGG ATTAAAGATG AAGAAGAAGC CAGTGAAGTA CACAGGTATT 
TCAGAAGAAG TGGACTACAG CATCCCTGAC TACAAGACTG TCATCAGCAT CAGCAGCAAG 
GACCAACTTA ATATCACCCT CTCCAAGTGT GGACTGGGCA TGCTCAGTAA CCTGGGCACG 
GCCTTTGCGG AGGCGGCCAA GCAGGCATCG GATAGTTTCC AGAAGGACGA GGCTCCGTTC 
GTCGTGAAGA ACCGTCTAGG TCTGACTGTG TCGGTGGTGC ACAGTGAGCT GTTCAGCCCC 
CTGGGTCGCG AGGCCACGGC GCGCCGGGTA GAGCTGCAGG ACGGAGAGAG CCTCAACATG 
GACTACCGTC GCACCACCAC CCACTCAGAC CAGTTCTCTG CCATGACTTC ATTGGTGGCC 
AAGGACTACT ACATACAGCC CACTCCGGTG GGCCACACTT CAACAGGCAT GATCCCGTTG 
ATCAAAGTGG GGCGTGGCAT GTACAGTGTC ATGCACAAAG ACTCTGGGGT CACCCGTTTC 
CTGGTCTGTC AGATCTACTC TGTGGAGGGA AGCAAGTACA TCAAGATCCG CTCTCCCTTC 
CAGATCATAA ACCACTTCTC CATCCCATTC AGAGTGATGG AGGGCTCTAC CAGTTTGGGG 
ATATCACTGC CTACAGAGGA GTTCTGTGTG CCTCTGGACT CCTACAGGTC AGAGCTAAGA 
CTGCAGCCGG TAACCGAGGG AGACGATCAG TACGACTGCT CCGAGGGTTT CAGCTACGAG 
TTGGTGAGCC AGCAGCCCGG TGCACGTGTG CAGCAGACCT GTCATCGCCA TGGTGACAGT 
GCTGGCGTCC TCATGGTCAA CATCGTCCCT CTGAAAGACA CGGTGACATC ACAACACACA 
GGAATGGCTG GGGAGCACTT TGACGAGCCC TACATCCTGC ACCTGTGGCC CTCCGTCCTG 
CTTCGGAACC TTCTGCCTTA CCCCATCACC TATCGCCTAC AGGACAGTGA AGAGCCCACC 
CCCATCACCC TGATGGACGG CCACTCGGCC CAGCTGCACA CCGCCATCAT GGACCAGTCC 
AGCCTGGAAC TCCGCCTCCT CGACTACCTG GGTCAGGATT GGCAGGCCCA CTACGGTCTC 
TGTGGCGACC TGGATGAGAT CACCTTCATT GTGTTCAGCT CTCTGCGCGA TGAAGAGGAG 
GTGGTCAGGT TAGGAGAGAG GGGAGAAGGG AAGAGGGCAG AGCTCGATAT TGCGGTGCAT 
GTGAAGTACG ACGCGGGCCA GACTGAGGTG GCTATCCATT CTCCCTACTG GATGGTGAAC 
AAGACGGGCC GCCTGCTGCA ATACAAGGCC GATGACATCC ACCGCAAACA CGCCATGGAC 
TACGATATGC CTCTGCTCTT CTCCTTCAAG CCACACAACT TTCTTCAGAA CAACAAGGTG 
CGGCTGATGA TCTCGGACAG CGACCTGTCG GATGATTTCT CCCTGGATAC CGTGGGTAGT 
TATGGTGAAG TGAAGTGCAA AGGGAGGCTG AAAGATTACG TGATCGGAGT GAAGATCGAC 
GCCAGCAGTT TCAGCCTGAC CCGTATCGTA AGCTTTGTGC CATACTACCT GTTAGTGAAC 
AGGACCAAAC ATGTGATCTG GGTGGGAGAG GAGGGCCAAG ACGACTGGAC CGAGGCCAGA 
CCACAACAGG CAGCCATCCC GTTTTGGCCT GAGAGGGACT CCAAGAAGCT CAAAGTCAAA 
GTAGAAGGCA GTCGGTCAAC TCCTCAAACC ATGGACTTCA CCAAACCGGA AAACTGCCTG 
CTGCTGCATC TGGACAACAC TCTGGGCGGG GTCATTATGG ATGTGAACCT ATCGGACCAC 
TCGATTATCA TCCGTTTCTC TGACTACCAC GACGGGGCCG CCCCCTTCCT CATCATCAAC 
CACACCCAGG AGCAGACGGT GGAGTTCTAC CAGAGATTTC GGAGAGATTC ATCAGAATGG 
GTGGCAGACG AGCTGCTCGG CCTCTCCTTC TCAGTAGAGG AGACCCAGCC CAGTAGTCAG 
AAGGAGAAGG ACAAGCTAAG CCCCGGGAAG GCAATGTACT ACACCTGGGC TGAGCCCACT 
GGATCGCGCT CTCTGGCCTG GCAGTGTGGC AGCTACAGCG GCGAGCTGAG GAGCGAAGAG 
GACCTGCGGT TGGACGTGAA CGAGGAGGGT AAGCTGTACC TCATCTCGTT CTACGAGGGC 
CTCCAGCGGG TGGCGCTGTT CACAGAGGAC AGACGCATAT ACAAGCTGCT GTGTGAGAGT 
GAGAAGGCCG AGCTGGCCGA ACAAGAGATC ATCCTCTCCC TGCAAGACGT GGGCGTCTCC 
CTGGTCAACA ACAGCAGCCG CCAGGAGGTG TCCTTCATCG GTATTACCAG TTCAGACGTG 
GTGTGGGAGT CCAAGCCAAA GAAGAAGTCT CGCTGGAAAC CCCTGAGTGG AAAAGATGCC 
GAGCTGCTGG AGACCCACTT CAAAGAGTAC ACAGAGAGTG GACCAGTGGA CAACGCCATC 
TTAGACCTGG AGAACAACTT CCAGGTGTCT TTGACCCATA ATGGGCAAGA CATGAGGATG 
ATGCAGCCAT ATGACGCTCC AATCAGGAGG AGCTTCCTGC CAGGGGTGAA GGTGGAGTAC 
AGCGTATCGG CCCGCCAGAG TGCCTACCGT GTCCAGATCA ACCGCATACA GATCCAGAAT 
CAGCTACCTG GAGCAATCTT CCCATATGTC TTCTACCCTA TAAAACTACC TAAGTCTGTC 
ACCATGGACT CAGAGCCCAA ACCTCTGACC GACATCAGCA TTGTTACCAG GGCAGCAGGG 
CACTCTGATA TCTCACGCAT CAAGTATTTC AAGGTGCTAA TCCAGGAGAT GGATCTGAAG 
TTGGACTTGG GCTTCCTTTA TGCCATCTTG GATCTGTTCA CTCCGGAGAA CGCCAGCGTG 
ATGACGTCAG AGCAGCAGGT GGAGTTGTTT GAGAAGGACA TAGAGTATCT GAAGACGGAG 
CTCAACGACG TCTCGGCCAC TGACACATCC CCTATCAGTC TCTATGAGTA CTTCCACATC 
TCTCCTATCA AGCTGCACCT CAGTTTCTCT CTGAGTTCTG GAGGAGAGGA TGGTAACAAG 
GAGAAGAGGG AGACCGAGAT CATCCCTGTC CAGTCCCTCA ATCTGCTGCT GAAAAGCATC 
GGAGCAACCC TCACTGACGT GCAGGATGTC GTCTTCAAAT TGGCCTTCTT CGAGCTGACC 
TTCCAGTTTC GCACTCGCCA GCAACTACAA TGGGAGGTCA TCAGACACTA CTCGAAACAG 
GCGATAAAGC AGATGTATGT GCTGGTGTTG GGCTTGGACG TGCTGGGAAA CCCCTTTGGA 
CTGATCCGAG GTCTGTCGGA GGGAGTGGAG GCTTTCTTCT ACGAGCCCTA TCAGGGAGCT 
ATCCAGGGGC CAGAGGAGTT TGTGGAGGGT ATGGCGCTGG GGGTGAAAGC GCTGGTTGGA 
GGAGCAGTAG GTGGCATTGC GGGCGCTGCC TCCAGGATCA CAGGCGCCAT GGCGAAGGGT 
GTGGCTGCCA TGACAATGGA CGAGGAGTAC CAGCAGAAGA GACGGGAGGC TATGAACAAG 
CAGCCTAGCG GCCTGAGAGA GGGCCTGACC CGCGGAGGCA AAGGACTGGT GTCTGGCTTT 
GTCAGTGGGA TCACAGGGAT TGTCACCAAG CCCATTAAAG GTGCTCAGAA GGAGGGCGCG 
GCGGGCTTCT TTAAGGGTGT GGGCAAGGGG CTAGTGGGTG CTGTGGCCAG ACCCACTGGA 
GGCATCATCG ACATGGCCAG CAGCACCTTC CAGGGCATCA AGAGGGCAGC TGAGACATCC 
CAGGATGTGG AGAGTCTGCG TCCGCCTCGC TTCATCCATG AGGACGGGGT CATCCGACCA 
TACAAGGAAA GAGAGGGCAT CGGCAGCCAG ATGCTACAGA AAATTGAAAA CGGAAGGTTC 
GCCAAGTACA GATACTTTGC GCACGCCAAA GTGAACGAGT CCGATTTCCT GATGATCACC 
AAAAGGGGAA TCTTTTTCGT GACCAAAGGT ACATTTGGGC AGCTGACCTG CGAGTGGCAG 
TATCTGTTCG ACGAGTTCAC CAAGGAGCCC ATGATCGTGG AGGGGAGGCG GCTTCGGATC 
GAGGCCAAGG AGAGAGTGAA GTCTGTGTTT CATGCCAAAG AGTTTGGGAA GATAATCAAC 
TTCAGAAGTC CAGAAATAGC CAAGTGGGTT CTTACCAAGC TTGAAGACGC CAGGGAGAGT 
CTACACAAAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021476710.1
CDS	274..10068
Translation	MVFESVVVDVLNRFLGDYVVNLDSSQLKLGIWGGDAVLRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDGVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEETKQKAAEQENPALEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPNCPFSFGVSLQNLSLQTADENWMPCLLDEKAKLFFKLVQLDNLFAYWNVNSMLYSNHMPDEALRCLQDSIPGGSTPMNHDFIFRPITADAKLRMNPRSDVDFSSPKVDLVVNLREVAVELNRPQYISILELLGSVDMMSRNLPYRKYRPQVHVHTNAYLWWQYVITGIMEVDVKPRLHMWSWQHIRCHRQTVKQYRELYKAKITSKKPTEKQLRELEEPERTLDIFNITLARQQAEMEASKAGLRIFRPGVKVEEEESQGWLGWMWSGWSGDCGAEAKEVKTGAFDELMTSAEKAKLYTAIGYSEIAVNHNLPKNFEDMKINFHMERMSVSMKDNKDQNEILKLTVEDLKSMLTQRPGAQAIKLSAQLSLFEVTGLASNRPAPTLLSSRKAATVTGTPLLDILFETNPLDESADQRLRVESQPLEIIYDAVTVNSMSAFFSPPDDLQLDDLTNATLMKLEQFRDRTSTGLMYVIETQKVLDLNVNLMASYVIVPQTGFHDSTQNLMLLDLGHFKVSSLSREGLPQLSVGRSTIEDIMSRAYDSFDVQLSSLQFLYSKPDGDWKKARKLRQSALHILEPVDVKVVFSRAMVVTDSRMPKFKISGELPLLSLRISDDKVRGVLELIDSIPLPESRPAPRSTNAHSTSKALKQSYTPQLTPRKPTMAELRNSLIWESGSEEDLFYDAPSSPIGDRPFFPDNMPSPLQRSDSVKRRGLVKKDPKKNMTEFLMTFEISKLSVQLCRLSGGEEVTVLHLDIEGLGTELKLRTFDMTSYTFLREICLECPEYMDSEEKKVHLITTLDNTEDDLLTLEYTKADKNGPEFKSEYKNTEQSVKVNFSSLDIHLHTEALLNAMNFLISLLPPTKEVAPSQEELPTIPEEDEGEGEEGEKKEGSAVAKKTSSKKSKFEDVVNLHIRADLNCLKVFIRGQSHRIAEIRIEGLVSEVLMRKKAVEVLANLKNIVILDCDKEALYKQCVSIADEEVFAFRMVNHTEATEGDAYLDMGKVDTSVTLTVGCIQVIFLNKFVSSILAFINNFQVAKEALAEVTVQAAEKAATGVMELAERSTRISLDVDFNAPVVFLPQSSSSANVIVADLGMVTVKNRFAMVPSQMHVKIPPVMDTMTVGLSDLKMYRTTFEEGRFQGEIQLLKPVNLDLSIQRNLSASWYHSIPDIEITAHLKPMNLILGQDDMTVVLRTLSGNLSETSDSASPPPPPRTVDRSDTASTKGSQASGGTGTNPTSGNTVVTAAVVETQKSTKLKSTLKLDFKFDSMTLVLYSPNGTEVQVLDSRDEQLKLAEFTLGTISTSVHMFSDGSMKASVKLTDCLLDDKRCRVKKITARMMAMRPGAERNVMVEVNYKQGRDGTSLDTVVQDVYLCASMEFLLTVADIFLKASQQGFSSATPKNSSSTKPTAAAASKESAPVGVVAKSEINVLVKNPEIVFVADLTRADAPALVMTTECELVMKSDLDGQLMTAVVKDLKVVACPFLKEKRNKETTVLQPCQVFFKSTQSSTSPQAMEVCINALTLKVSPIIINTVITIQSVLSPAGAETPQSELESPVPPDLWERRSWRDLKLWFLEEEGKDEDAQSLVPLIPQGESLQLAINSICLTLEAGVGHRTVPMLLAKSSFHGDVKNWSTLINLHSQLNLEVHYFNEVMGVWEPLLEPLEDETRDGFRPWTLGLKMKKKPVKYTGISEEVDYSIPDYKTVISISSKDQLNITLSKCGLGMLSNLGTAFAEAAKQASDSFQKDEAPFVVKNRLGLTVSVVHSELFSPLGREATARRVELQDGESLNMDYRRTTTHSDQFSAMTSLVAKDYYIQPTPVGHTSTGMIPLIKVGRGMYSVMHKDSGVTRFLVCQIYSVEGSKYIKIRSPFQIINHFSIPFRVMEGSTSLGISLPTEEFCVPLDSYRSELRLQPVTEGDDQYDCSEGFSYELVSQQPGARVQQTCHRHGDSAGVLMVNIVPLKDTVTSQHTGMAGEHFDEPYILHLWPSVLLRNLLPYPITYRLQDSEEPTPITLMDGHSAQLHTAIMDQSSLELRLLDYLGQDWQAHYGLCGDLDEITFIVFSSLRDEEEVVRLGERGEGKRAELDIAVHVKYDAGQTEVAIHSPYWMVNKTGRLLQYKADDIHRKHAMDYDMPLLFSFKPHNFLQNNKVRLMISDSDLSDDFSLDTVGSYGEVKCKGRLKDYVIGVKIDASSFSLTRIVSFVPYYLLVNRTKHVIWVGEEGQDDWTEARPQQAAIPFWPERDSKKLKVKVEGSRSTPQTMDFTKPENCLLLHLDNTLGGVIMDVNLSDHSIIIRFSDYHDGAAPFLIINHTQEQTVEFYQRFRRDSSEWVADELLGLSFSVEETQPSSQKEKDKLSPGKAMYYTWAEPTGSRSLAWQCGSYSGELRSEEDLRLDVNEEGKLYLISFYEGLQRVALFTEDRRIYKLLCESEKAELAEQEIILSLQDVGVSLVNNSSRQEVSFIGITSSDVVWESKPKKKSRWKPLSGKDAELLETHFKEYTESGPVDNAILDLENNFQVSLTHNGQDMRMMQPYDAPIRRSFLPGVKVEYSVSARQSAYRVQINRIQIQNQLPGAIFPYVFYPIKLPKSVTMDSEPKPLTDISIVTRAAGHSDISRIKYFKVLIQEMDLKLDLGFLYAILDLFTPENASVMTSEQQVELFEKDIEYLKTELNDVSATDTSPISLYEYFHISPIKLHLSFSLSSGGEDGNKEKRETEIIPVQSLNLLLKSIGATLTDVQDVVFKLAFFELTFQFRTRQQLQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGIAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRAAETSQDVESLRPPRFIHEDGVIRPYKEREGIGSQMLQKIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKGTFGQLTCEWQYLFDEFTKEPMIVEGRRLRIEAKERVKSVFHAKEFGKIINFRSPEIAKWVLTKLEDARESLHKY

Target ORF information:

RefSeq Version	XM_021621035.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621035.1

ORF Insert Sequence:

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGT 
ACTGGTACTA ACCCCACCAG CGGCAACACG GTGGTAACTG CTGCAGTGGT GGAGACCCAG 
AAGAGCACCA AGCTGAAGAG CACACTCAAA CTGGACTTCA AGTTTGACTC CATGACCCTG 
GTCCTGTACA GCCCCAATGG GACTGAGGTG CAGGTGTTGG ACTCGAGGGA TGAGCAGCTG 
AAGCTGGCTG AGTTTACCCT GGGGACTATC TCTACATCTG TCCACATGTT CTCAGACGGA 
TCTATGAAGG CCTCCGTCAA ACTCACAGAC TGCCTGCTAG ACGACAAAAG ATGCAGGGTC 
AAGAAGATCA CTGCCAGGAT GATGGCGATG CGTCCGGGCG CTGAGAGGAA CGTGATGGTG 
GAGGTGAACT ACAAGCAGGG CCGCGACGGC ACCTCTTTGG ACACGGTGGT GCAGGACGTG 
TACCTCTGTG CCAGCATGGA GTTCCTGCTC ACTGTGGCCG ACATCTTCCT CAAAGCCAGC 
CAGCAGGGCT TCTCCTCCGC CACCCCCAAG AACAGCAGTA GCACTAAACC CACTGCAGCC 
GCAGCCTCCA AGGAATCTGC TCCAGTGGGG GTGGTGGCAA AGTCAGAGAT TAATGTTCTG 
GTGAAGAACC CCGAGATTGT GTTTGTGGCC GACCTGACAC GTGCCGACGC CCCGGCCCTG 
GTCATGACCA CGGAGTGTGA GCTGGTGATG AAGAGCGACC TGGACGGTCA GCTGATGACT 
GCGGTGGTCA AGGACCTGAA GGTGGTGGCC TGCCCCTTCC TGAAGGAGAA GAGAAACAAG 
GAGACCACTG TGCTGCAGCC CTGCCAAGTG TTCTTCAAGA GCACACAGTC CTCCACCTCC 
CCGCAGGCCA TGGAGGTCTG CATCAACGCA CTCACACTCA AGGTGTCGCC CATCATTATC 
AACACAGTGA TTACCATTCA GTCGGTGCTG AGCCCGGCCG GTGCCGAGAC ACCCCAGTCT 
GAGCTAGAGA GCCCCGTGCC CCCAGACCTG TGGGAGCGCC GGTCCTGGAG GGACCTCAAG 
CTGTGGTTCC TGGAGGAGGA GGGTAAAGAC GAGGATGCCC AGTCCCTGGT CCCGCTGATT 
CCCCAGGGCG AGTCCCTGCA GTTGGCCATC AACTCCATCT GCCTGACACT GGAGGCAGGG 
GTGGGCCACC GCACCGTCCC CATGCTCCTG GCCAAGTCCT CCTTCCACGG TGACGTCAAG 
AACTGGAGCA CCCTCATCAA CCTGCACAGC CAGCTCAACC TGGAGGTTCA CTACTTCAAC 
GAGGTGATGG GGGTGTGGGA GCCACTGCTA GAGCCCCTAG AAGACGAGAC CCGAGATGGA 
TTCAGACCCT GGACCCTGGG ATTAAAGATG AAGAAGAAGC CAGTGAAGTA CACAGGTATT 
TCAGAAGAAG TGGACTACAG CATCCCTGAC TACAAGACTG TCATCAGCAT CAGCAGCAAG 
GACCAACTTA ATATCACCCT CTCCAAGTGT GGACTGGGCA TGCTCAGTAA CCTGGGCACG 
GCCTTTGCGG AGGCGGCCAA GCAGGCATCG GATAGTTTCC AGAAGGACGA GGCTCCGTTC 
GTCGTGAAGA ACCGTCTAGG TCTGACTGTG TCGGTGGTGC ACAGTGAGCT GTTCAGCCCC 
CTGGGTCGCG AGGCCACGGC GCGCCGGGTA GAGCTGCAGG ACGGAGAGAG CCTCAACATG 
GACTACCGTC GCACCACCAC CCACTCAGAC CAGTTCTCTG CCATGACTTC ATTGGTGGCC 
AAGGACTACT ACATACAGCC CACTCCGGTG GGCCACACTT CAACAGGCAT GATCCCGTTG 
ATCAAAGTGG GGCGTGGCAT GTACAGTGTC ATGCACAAAG ACTCTGGGGT CACCCGTTTC 
CTGGTCTGTC AGATCTACTC TGTGGAGGGA AGCAAGTACA TCAAGATCCG CTCTCCCTTC 
CAGATCATAA ACCACTTCTC CATCCCATTC AGAGTGATGG AGGGCTCTAC CAGTTTGGGG 
ATATCACTGC CTACAGAGGA GTTCTGTGTG CCTCTGGACT CCTACAGGTC AGAGCTAAGA 
CTGCAGCCGG TAACCGAGGG AGACGATCAG TACGACTGCT CCGAGGGTTT CAGCTACGAG 
TTGGTGAGCC AGCAGCCCGG TGCACGTGTG CAGCAGACCT GTCATCGCCA TGGTGACAGT 
GCTGGCGTCC TCATGGTCAA CATCGTCCCT CTGAAAGACA CGGTGACATC ACAACACACA 
GGAATGGCTG GGGAGCACTT TGACGAGCCC TACATCCTGC ACCTGTGGCC CTCCGTCCTG 
CTTCGGAACC TTCTGCCTTA CCCCATCACC TATCGCCTAC AGGACAGTGA AGAGCCCACC 
CCCATCACCC TGATGGACGG CCACTCGGCC CAGCTGCACA CCGCCATCAT GGACCAGTCC 
AGCCTGGAAC TCCGCCTCCT CGACTACCTG GGTCAGGATT GGCAGGCCCA CTACGGTCTC 
TGTGGCGACC TGGATGAGAT CACCTTCATT GTGTTCAGCT CTCTGCGCGA TGAAGAGGAG 
GTGGTCAGGT TAGGAGAGAG GGGAGAAGGG AAGAGGGCAG AGCTCGATAT TGCGGTGCAT 
GTGAAGTACG ACGCGGGCCA GACTGAGGTG GCTATCCATT CTCCCTACTG GATGGTGAAC 
AAGACGGGCC GCCTGCTGCA ATACAAGGCC GATGACATCC ACCGCAAACA CGCCATGGAC 
TACGATATGC CTCTGCTCTT CTCCTTCAAG CCACACAACT TTCTTCAGAA CAACAAGGTG 
CGGCTGATGA TCTCGGACAG CGACCTGTCG GATGATTTCT CCCTGGATAC CGTGGGTAGT 
TATGGTGAAG TGAAGTGCAA AGGGAGGCTG AAAGATTACG TGATCGGAGT GAAGATCGAC 
GCCAGCAGTT TCAGCCTGAC CCGTATCGTA AGCTTTGTGC CATACTACCT GTTAGTGAAC 
AGGACCAAAC ATGTGATCTG GGTGGGAGAG GAGGGCCAAG ACGACTGGAC CGAGGCCAGA 
CCACAACAGG CAGCCATCCC GTTTTGGCCT GAGAGGGACT CCAAGAAGCT CAAAGTCAAA 
GTAGAAGGCA GTCGGTCAAC TCCTCAAACC ATGGACTTCA CCAAACCGGA AAACTGCCTG 
CTGCTGCATC TGGACAACAC TCTGGGCGGG GTCATTATGG ATGTGAACCT ATCGGACCAC 
TCGATTATCA TCCGTTTCTC TGACTACCAC GACGGGGCCG CCCCCTTCCT CATCATCAAC 
CACACCCAGG AGCAGACGGT GGAGTTCTAC CAGAGATTTC GGAGAGATTC ATCAGAATGG 
GTGGCAGACG AGCTGCTCGG CCTCTCCTTC TCAGTAGAGG AGACCCAGCC CAGTAGTCAG 
AAGGAGAAGG ACAAGCTAAG CCCCGGGAAG GCAATGTACT ACACCTGGGC TGAGCCCACT 
GGATCGCGCT CTCTGGCCTG GCAGTGTGGC AGCTACAGCG GCGAGCTGAG GAGCGAAGAG 
GACCTGCGGT TGGACGTGAA CGAGGAGGGT AAGCTGTACC TCATCTCGTT CTACGAGGGC 
CTCCAGCGGG TGGCGCTGTT CACAGAGGAC AGACGCATAT ACAAGCTGCT GTGTGAGAGT 
GAGAAGGCCG AGCTGGCCGA ACAAGAGATC ATCCTCTCCC TGCAAGACGT GGGCGTCTCC 
CTGGTCAACA ACAGCAGCCG CCAGGAGGTG TCCTTCATCG GTATTACCAG TTCAGACGTG 
GTGTGGGAGT CCAAGCCAAA GAAGAAGTCT CGCTGGAAAC CCCTGAGTGG AAAAGATGCC 
GAGCTGCTGG AGACCCACTT CAAAGAGTAC ACAGAGAGTG GACCAGTGGA CAACGCCATC 
TTAGACCTGG AGAACAACTT CCAGGTGTCT TTGACCCATA ATGGGCAAGA CATGAGGATG 
ATGCAGCCAT ATGACGCTCC AATCAGGAGG AGCTTCCTGC CAGGGGTGAA GGTGGAGTAC 
AGCGTATCGG CCCGCCAGAG TGCCTACCGT GTCCAGATCA ACCGCATACA GATCCAGAAT 
CAGCTACCTG GAGCAATCTT CCCATATGTC TTCTACCCTA TAAAACTACC TAAGTCTGTC 
ACCATGGACT CAGAGCCCAA ACCTCTGACC GACATCAGCA TTGTTACCAG GGCAGCAGGG 
CACTCTGATA TCTCACGCAT CAAGTATTTC AAGGTGCTAA TCCAGGAGAT GGATCTGAAG 
TTGGACTTGG GCTTCCTTTA TGCCATCTTG GATCTGTTCA CTCCGGAGAA CGCCAGCGTG 
ATGACGTCAG AGCAGCAGGT GGAGTTGTTT GAGAAGGACA TAGAGTATCT GAAGACGGAG 
CTCAACGACG TCTCGGCCAC TGACACATCC CCTATCAGTC TCTATGAGTA CTTCCACATC 
TCTCCTATCA AGCTGCACCT CAGTTTCTCT CTGAGTTCTG GAGGAGAGGA TGGTAACAAG 
GAGAAGAGGG AGACCGAGAT CATCCCTGTC CAGTCCCTCA ATCTGCTGCT GAAAAGCATC 
GGAGCAACCC TCACTGACGT GCAGGATGTC GTCTTCAAAT TGGCCTTCTT CGAGCTGACC 
TTCCAGTTTC GCACTCGCCA GCAACTACAA TGGGAGGTCA TCAGACACTA CTCGAAACAG 
GCGATAAAGC AGATGTATGT GCTGGTGTTG GGCTTGGACG TGCTGGGAAA CCCCTTTGGA 
CTGATCCGAG GTCTGTCGGA GGGAGTGGAG GCTTTCTTCT ACGAGCCCTA TCAGGGAGCT 
ATCCAGGGGC CAGAGGAGTT TGTGGAGGGT ATGGCGCTGG GGGTGAAAGC GCTGGTTGGA 
GGAGCAGTAG GTGGCATTGC GGGCGCTGCC TCCAGGATCA CAGGCGCCAT GGCGAAGGGT 
GTGGCTGCCA TGACAATGGA CGAGGAGTAC CAGCAGAAGA GACGGGAGGC TATGAACAAG 
CAGCCTAGCG GCCTGAGAGA GGGCCTGACC CGCGGAGGCA AAGGACTGGT GTCTGGCTTT 
GTCAGTGGGA TCACAGGGAT TGTCACCAAG CCCATTAAAG GTGCTCAGAA GGAGGGCGCG 
GCGGGCTTCT TTAAGGGTGT GGGCAAGGGG CTAGTGGGTG CTGTGGCCAG ACCCACTGGA 
GGCATCATCG ACATGGCCAG CAGCACCTTC CAGGGCATCA AGAGGGCAGC TGAGACATCC 
CAGGATGTGG AGAGTCTGCG TCCGCCTCGC TTCATCCATG AGGACGGGGT CATCCGACCA 
TACAAGGAAA GAGAGGGCAT CGGCAGCCAG ATGCTACAGA AAATTGAAAA CGGAAGGTTC 
GCCAAGTACA GATACTTTGC GCACGCCAAA GTGAACGAGT CCGATTTCCT GATGATCACC 
AAAAGGGGAA TCTTTTTCGT GACCAAAGGT ACATTTGGGC AGCTGACCTG CGAGTGGCAG 
TATCTGTTCG ACGAGTTCAC CAAGGAGCCC ATGATCGTGG AGGGGAGGCG GCTTCGGATC 
GAGGCCAAGG AGAGAGTGAA GTCTGTGTTT CATGCCAAAG AGTTTGGGAA GATAATCAAC 
TTCAGAAGTC CAGAAATAGC CAAGTGGGTT CTTACCAAGC TTGAAGACGC CAGGGAGAGT 
CTACACAAAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps13a ( XM_021621036.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021476711.1 Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X2, mRNA.

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CloneID	OOf56504
Clone ID Related Accession (Same CDS sequence)	XM_021621036.1
Accession Version	XM_021621036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	vacuolar protein sorting-associated protein 13A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGT 
ACTGGTACTA ACCCCACCAG CGGCAACACG GTGGTAACTG CTGCAGTGGT GGAGACCCAG 
AAGAGCACCA AGCTGAAGAG CACACTCAAA CTGGACTTCA AGTTTGACTC CATGACCCTG 
GTCCTGTACA GCCCCAATGG GACTGAGGTG TTGGACTCGA GGGATGAGCA GCTGAAGCTG 
GCTGAGTTTA CCCTGGGGAC TATCTCTACA TCTGTCCACA TGTTCTCAGA CGGATCTATG 
AAGGCCTCCG TCAAACTCAC AGACTGCCTG CTAGACGACA AAAGATGCAG GGTCAAGAAG 
ATCACTGCCA GGATGATGGC GATGCGTCCG GGCGCTGAGA GGAACGTGAT GGTGGAGGTG 
AACTACAAGC AGGGCCGCGA CGGCACCTCT TTGGACACGG TGGTGCAGGA CGTGTACCTC 
TGTGCCAGCA TGGAGTTCCT GCTCACTGTG GCCGACATCT TCCTCAAAGC CAGCCAGCAG 
GGCTTCTCCT CCGCCACCCC CAAGAACAGC AGTAGCACTA AACCCACTGC AGCCGCAGCC 
TCCAAGGAAT CTGCTCCAGT GGGGGTGGTG GCAAAGTCAG AGATTAATGT TCTGGTGAAG 
AACCCCGAGA TTGTGTTTGT GGCCGACCTG ACACGTGCCG ACGCCCCGGC CCTGGTCATG 
ACCACGGAGT GTGAGCTGGT GATGAAGAGC GACCTGGACG GTCAGCTGAT GACTGCGGTG 
GTCAAGGACC TGAAGGTGGT GGCCTGCCCC TTCCTGAAGG AGAAGAGAAA CAAGGAGACC 
ACTGTGCTGC AGCCCTGCCA AGTGTTCTTC AAGAGCACAC AGTCCTCCAC CTCCCCGCAG 
GCCATGGAGG TCTGCATCAA CGCACTCACA CTCAAGGTGT CGCCCATCAT TATCAACACA 
GTGATTACCA TTCAGTCGGT GCTGAGCCCG GCCGGTGCCG AGACACCCCA GTCTGAGCTA 
GAGAGCCCCG TGCCCCCAGA CCTGTGGGAG CGCCGGTCCT GGAGGGACCT CAAGCTGTGG 
TTCCTGGAGG AGGAGGGTAA AGACGAGGAT GCCCAGTCCC TGGTCCCGCT GATTCCCCAG 
GGCGAGTCCC TGCAGTTGGC CATCAACTCC ATCTGCCTGA CACTGGAGGC AGGGGTGGGC 
CACCGCACCG TCCCCATGCT CCTGGCCAAG TCCTCCTTCC ACGGTGACGT CAAGAACTGG 
AGCACCCTCA TCAACCTGCA CAGCCAGCTC AACCTGGAGG TTCACTACTT CAACGAGGTG 
ATGGGGGTGT GGGAGCCACT GCTAGAGCCC CTAGAAGACG AGACCCGAGA TGGATTCAGA 
CCCTGGACCC TGGGATTAAA GATGAAGAAG AAGCCAGTGA AGTACACAGG TATTTCAGAA 
GAAGTGGACT ACAGCATCCC TGACTACAAG ACTGTCATCA GCATCAGCAG CAAGGACCAA 
CTTAATATCA CCCTCTCCAA GTGTGGACTG GGCATGCTCA GTAACCTGGG CACGGCCTTT 
GCGGAGGCGG CCAAGCAGGC ATCGGATAGT TTCCAGAAGG ACGAGGCTCC GTTCGTCGTG 
AAGAACCGTC TAGGTCTGAC TGTGTCGGTG GTGCACAGTG AGCTGTTCAG CCCCCTGGGT 
CGCGAGGCCA CGGCGCGCCG GGTAGAGCTG CAGGACGGAG AGAGCCTCAA CATGGACTAC 
CGTCGCACCA CCACCCACTC AGACCAGTTC TCTGCCATGA CTTCATTGGT GGCCAAGGAC 
TACTACATAC AGCCCACTCC GGTGGGCCAC ACTTCAACAG GCATGATCCC GTTGATCAAA 
GTGGGGCGTG GCATGTACAG TGTCATGCAC AAAGACTCTG GGGTCACCCG TTTCCTGGTC 
TGTCAGATCT ACTCTGTGGA GGGAAGCAAG TACATCAAGA TCCGCTCTCC CTTCCAGATC 
ATAAACCACT TCTCCATCCC ATTCAGAGTG ATGGAGGGCT CTACCAGTTT GGGGATATCA 
CTGCCTACAG AGGAGTTCTG TGTGCCTCTG GACTCCTACA GGTCAGAGCT AAGACTGCAG 
CCGGTAACCG AGGGAGACGA TCAGTACGAC TGCTCCGAGG GTTTCAGCTA CGAGTTGGTG 
AGCCAGCAGC CCGGTGCACG TGTGCAGCAG ACCTGTCATC GCCATGGTGA CAGTGCTGGC 
GTCCTCATGG TCAACATCGT CCCTCTGAAA GACACGGTGA CATCACAACA CACAGGAATG 
GCTGGGGAGC ACTTTGACGA GCCCTACATC CTGCACCTGT GGCCCTCCGT CCTGCTTCGG 
AACCTTCTGC CTTACCCCAT CACCTATCGC CTACAGGACA GTGAAGAGCC CACCCCCATC 
ACCCTGATGG ACGGCCACTC GGCCCAGCTG CACACCGCCA TCATGGACCA GTCCAGCCTG 
GAACTCCGCC TCCTCGACTA CCTGGGTCAG GATTGGCAGG CCCACTACGG TCTCTGTGGC 
GACCTGGATG AGATCACCTT CATTGTGTTC AGCTCTCTGC GCGATGAAGA GGAGGTGGTC 
AGGTTAGGAG AGAGGGGAGA AGGGAAGAGG GCAGAGCTCG ATATTGCGGT GCATGTGAAG 
TACGACGCGG GCCAGACTGA GGTGGCTATC CATTCTCCCT ACTGGATGGT GAACAAGACG 
GGCCGCCTGC TGCAATACAA GGCCGATGAC ATCCACCGCA AACACGCCAT GGACTACGAT 
ATGCCTCTGC TCTTCTCCTT CAAGCCACAC AACTTTCTTC AGAACAACAA GGTGCGGCTG 
ATGATCTCGG ACAGCGACCT GTCGGATGAT TTCTCCCTGG ATACCGTGGG TAGTTATGGT 
GAAGTGAAGT GCAAAGGGAG GCTGAAAGAT TACGTGATCG GAGTGAAGAT CGACGCCAGC 
AGTTTCAGCC TGACCCGTAT CGTAAGCTTT GTGCCATACT ACCTGTTAGT GAACAGGACC 
AAACATGTGA TCTGGGTGGG AGAGGAGGGC CAAGACGACT GGACCGAGGC CAGACCACAA 
CAGGCAGCCA TCCCGTTTTG GCCTGAGAGG GACTCCAAGA AGCTCAAAGT CAAAGTAGAA 
GGCAGTCGGT CAACTCCTCA AACCATGGAC TTCACCAAAC CGGAAAACTG CCTGCTGCTG 
CATCTGGACA ACACTCTGGG CGGGGTCATT ATGGATGTGA ACCTATCGGA CCACTCGATT 
ATCATCCGTT TCTCTGACTA CCACGACGGG GCCGCCCCCT TCCTCATCAT CAACCACACC 
CAGGAGCAGA CGGTGGAGTT CTACCAGAGA TTTCGGAGAG ATTCATCAGA ATGGGTGGCA 
GACGAGCTGC TCGGCCTCTC CTTCTCAGTA GAGGAGACCC AGCCCAGTAG TCAGAAGGAG 
AAGGACAAGC TAAGCCCCGG GAAGGCAATG TACTACACCT GGGCTGAGCC CACTGGATCG 
CGCTCTCTGG CCTGGCAGTG TGGCAGCTAC AGCGGCGAGC TGAGGAGCGA AGAGGACCTG 
CGGTTGGACG TGAACGAGGA GGGTAAGCTG TACCTCATCT CGTTCTACGA GGGCCTCCAG 
CGGGTGGCGC TGTTCACAGA GGACAGACGC ATATACAAGC TGCTGTGTGA GAGTGAGAAG 
GCCGAGCTGG CCGAACAAGA GATCATCCTC TCCCTGCAAG ACGTGGGCGT CTCCCTGGTC 
AACAACAGCA GCCGCCAGGA GGTGTCCTTC ATCGGTATTA CCAGTTCAGA CGTGGTGTGG 
GAGTCCAAGC CAAAGAAGAA GTCTCGCTGG AAACCCCTGA GTGGAAAAGA TGCCGAGCTG 
CTGGAGACCC ACTTCAAAGA GTACACAGAG AGTGGACCAG TGGACAACGC CATCTTAGAC 
CTGGAGAACA ACTTCCAGGT GTCTTTGACC CATAATGGGC AAGACATGAG GATGATGCAG 
CCATATGACG CTCCAATCAG GAGGAGCTTC CTGCCAGGGG TGAAGGTGGA GTACAGCGTA 
TCGGCCCGCC AGAGTGCCTA CCGTGTCCAG ATCAACCGCA TACAGATCCA GAATCAGCTA 
CCTGGAGCAA TCTTCCCATA TGTCTTCTAC CCTATAAAAC TACCTAAGTC TGTCACCATG 
GACTCAGAGC CCAAACCTCT GACCGACATC AGCATTGTTA CCAGGGCAGC AGGGCACTCT 
GATATCTCAC GCATCAAGTA TTTCAAGGTG CTAATCCAGG AGATGGATCT GAAGTTGGAC 
TTGGGCTTCC TTTATGCCAT CTTGGATCTG TTCACTCCGG AGAACGCCAG CGTGATGACG 
TCAGAGCAGC AGGTGGAGTT GTTTGAGAAG GACATAGAGT ATCTGAAGAC GGAGCTCAAC 
GACGTCTCGG CCACTGACAC ATCCCCTATC AGTCTCTATG AGTACTTCCA CATCTCTCCT 
ATCAAGCTGC ACCTCAGTTT CTCTCTGAGT TCTGGAGGAG AGGATGGTAA CAAGGAGAAG 
AGGGAGACCG AGATCATCCC TGTCCAGTCC CTCAATCTGC TGCTGAAAAG CATCGGAGCA 
ACCCTCACTG ACGTGCAGGA TGTCGTCTTC AAATTGGCCT TCTTCGAGCT GACCTTCCAG 
TTTCGCACTC GCCAGCAACT ACAATGGGAG GTCATCAGAC ACTACTCGAA ACAGGCGATA 
AAGCAGATGT ATGTGCTGGT GTTGGGCTTG GACGTGCTGG GAAACCCCTT TGGACTGATC 
CGAGGTCTGT CGGAGGGAGT GGAGGCTTTC TTCTACGAGC CCTATCAGGG AGCTATCCAG 
GGGCCAGAGG AGTTTGTGGA GGGTATGGCG CTGGGGGTGA AAGCGCTGGT TGGAGGAGCA 
GTAGGTGGCA TTGCGGGCGC TGCCTCCAGG ATCACAGGCG CCATGGCGAA GGGTGTGGCT 
GCCATGACAA TGGACGAGGA GTACCAGCAG AAGAGACGGG AGGCTATGAA CAAGCAGCCT 
AGCGGCCTGA GAGAGGGCCT GACCCGCGGA GGCAAAGGAC TGGTGTCTGG CTTTGTCAGT 
GGGATCACAG GGATTGTCAC CAAGCCCATT AAAGGTGCTC AGAAGGAGGG CGCGGCGGGC 
TTCTTTAAGG GTGTGGGCAA GGGGCTAGTG GGTGCTGTGG CCAGACCCAC TGGAGGCATC 
ATCGACATGG CCAGCAGCAC CTTCCAGGGC ATCAAGAGGG CAGCTGAGAC ATCCCAGGAT 
GTGGAGAGTC TGCGTCCGCC TCGCTTCATC CATGAGGACG GGGTCATCCG ACCATACAAG 
GAAAGAGAGG GCATCGGCAG CCAGATGCTA CAGAAAATTG AAAACGGAAG GTTCGCCAAG 
TACAGATACT TTGCGCACGC CAAAGTGAAC GAGTCCGATT TCCTGATGAT CACCAAAAGG 
GGAATCTTTT TCGTGACCAA AGGTACATTT GGGCAGCTGA CCTGCGAGTG GCAGTATCTG 
TTCGACGAGT TCACCAAGGA GCCCATGATC GTGGAGGGGA GGCGGCTTCG GATCGAGGCC 
AAGGAGAGAG TGAAGTCTGT GTTTCATGCC AAAGAGTTTG GGAAGATAAT CAACTTCAGA 
AGTCCAGAAA TAGCCAAGTG GGTTCTTACC AAGCTTGAAG ACGCCAGGGA GAGTCTACAC 
AAATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021476711.1
CDS	274..10062
Translation	MVFESVVVDVLNRFLGDYVVNLDSSQLKLGIWGGDAVLRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDGVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEETKQKAAEQENPALEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPNCPFSFGVSLQNLSLQTADENWMPCLLDEKAKLFFKLVQLDNLFAYWNVNSMLYSNHMPDEALRCLQDSIPGGSTPMNHDFIFRPITADAKLRMNPRSDVDFSSPKVDLVVNLREVAVELNRPQYISILELLGSVDMMSRNLPYRKYRPQVHVHTNAYLWWQYVITGIMEVDVKPRLHMWSWQHIRCHRQTVKQYRELYKAKITSKKPTEKQLRELEEPERTLDIFNITLARQQAEMEASKAGLRIFRPGVKVEEEESQGWLGWMWSGWSGDCGAEAKEVKTGAFDELMTSAEKAKLYTAIGYSEIAVNHNLPKNFEDMKINFHMERMSVSMKDNKDQNEILKLTVEDLKSMLTQRPGAQAIKLSAQLSLFEVTGLASNRPAPTLLSSRKAATVTGTPLLDILFETNPLDESADQRLRVESQPLEIIYDAVTVNSMSAFFSPPDDLQLDDLTNATLMKLEQFRDRTSTGLMYVIETQKVLDLNVNLMASYVIVPQTGFHDSTQNLMLLDLGHFKVSSLSREGLPQLSVGRSTIEDIMSRAYDSFDVQLSSLQFLYSKPDGDWKKARKLRQSALHILEPVDVKVVFSRAMVVTDSRMPKFKISGELPLLSLRISDDKVRGVLELIDSIPLPESRPAPRSTNAHSTSKALKQSYTPQLTPRKPTMAELRNSLIWESGSEEDLFYDAPSSPIGDRPFFPDNMPSPLQRSDSVKRRGLVKKDPKKNMTEFLMTFEISKLSVQLCRLSGGEEVTVLHLDIEGLGTELKLRTFDMTSYTFLREICLECPEYMDSEEKKVHLITTLDNTEDDLLTLEYTKADKNGPEFKSEYKNTEQSVKVNFSSLDIHLHTEALLNAMNFLISLLPPTKEVAPSQEELPTIPEEDEGEGEEGEKKEGSAVAKKTSSKKSKFEDVVNLHIRADLNCLKVFIRGQSHRIAEIRIEGLVSEVLMRKKAVEVLANLKNIVILDCDKEALYKQCVSIADEEVFAFRMVNHTEATEGDAYLDMGKVDTSVTLTVGCIQVIFLNKFVSSILAFINNFQVAKEALAEVTVQAAEKAATGVMELAERSTRISLDVDFNAPVVFLPQSSSSANVIVADLGMVTVKNRFAMVPSQMHVKIPPVMDTMTVGLSDLKMYRTTFEEGRFQGEIQLLKPVNLDLSIQRNLSASWYHSIPDIEITAHLKPMNLILGQDDMTVVLRTLSGNLSETSDSASPPPPPRTVDRSDTASTKGSQASGGTGTNPTSGNTVVTAAVVETQKSTKLKSTLKLDFKFDSMTLVLYSPNGTEVLDSRDEQLKLAEFTLGTISTSVHMFSDGSMKASVKLTDCLLDDKRCRVKKITARMMAMRPGAERNVMVEVNYKQGRDGTSLDTVVQDVYLCASMEFLLTVADIFLKASQQGFSSATPKNSSSTKPTAAAASKESAPVGVVAKSEINVLVKNPEIVFVADLTRADAPALVMTTECELVMKSDLDGQLMTAVVKDLKVVACPFLKEKRNKETTVLQPCQVFFKSTQSSTSPQAMEVCINALTLKVSPIIINTVITIQSVLSPAGAETPQSELESPVPPDLWERRSWRDLKLWFLEEEGKDEDAQSLVPLIPQGESLQLAINSICLTLEAGVGHRTVPMLLAKSSFHGDVKNWSTLINLHSQLNLEVHYFNEVMGVWEPLLEPLEDETRDGFRPWTLGLKMKKKPVKYTGISEEVDYSIPDYKTVISISSKDQLNITLSKCGLGMLSNLGTAFAEAAKQASDSFQKDEAPFVVKNRLGLTVSVVHSELFSPLGREATARRVELQDGESLNMDYRRTTTHSDQFSAMTSLVAKDYYIQPTPVGHTSTGMIPLIKVGRGMYSVMHKDSGVTRFLVCQIYSVEGSKYIKIRSPFQIINHFSIPFRVMEGSTSLGISLPTEEFCVPLDSYRSELRLQPVTEGDDQYDCSEGFSYELVSQQPGARVQQTCHRHGDSAGVLMVNIVPLKDTVTSQHTGMAGEHFDEPYILHLWPSVLLRNLLPYPITYRLQDSEEPTPITLMDGHSAQLHTAIMDQSSLELRLLDYLGQDWQAHYGLCGDLDEITFIVFSSLRDEEEVVRLGERGEGKRAELDIAVHVKYDAGQTEVAIHSPYWMVNKTGRLLQYKADDIHRKHAMDYDMPLLFSFKPHNFLQNNKVRLMISDSDLSDDFSLDTVGSYGEVKCKGRLKDYVIGVKIDASSFSLTRIVSFVPYYLLVNRTKHVIWVGEEGQDDWTEARPQQAAIPFWPERDSKKLKVKVEGSRSTPQTMDFTKPENCLLLHLDNTLGGVIMDVNLSDHSIIIRFSDYHDGAAPFLIINHTQEQTVEFYQRFRRDSSEWVADELLGLSFSVEETQPSSQKEKDKLSPGKAMYYTWAEPTGSRSLAWQCGSYSGELRSEEDLRLDVNEEGKLYLISFYEGLQRVALFTEDRRIYKLLCESEKAELAEQEIILSLQDVGVSLVNNSSRQEVSFIGITSSDVVWESKPKKKSRWKPLSGKDAELLETHFKEYTESGPVDNAILDLENNFQVSLTHNGQDMRMMQPYDAPIRRSFLPGVKVEYSVSARQSAYRVQINRIQIQNQLPGAIFPYVFYPIKLPKSVTMDSEPKPLTDISIVTRAAGHSDISRIKYFKVLIQEMDLKLDLGFLYAILDLFTPENASVMTSEQQVELFEKDIEYLKTELNDVSATDTSPISLYEYFHISPIKLHLSFSLSSGGEDGNKEKRETEIIPVQSLNLLLKSIGATLTDVQDVVFKLAFFELTFQFRTRQQLQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGIAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRAAETSQDVESLRPPRFIHEDGVIRPYKEREGIGSQMLQKIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKGTFGQLTCEWQYLFDEFTKEPMIVEGRRLRIEAKERVKSVFHAKEFGKIINFRSPEIAKWVLTKLEDARESLHKY

Target ORF information:

RefSeq Version	XM_021621036.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621036.1

ORF Insert Sequence:

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGT 
ACTGGTACTA ACCCCACCAG CGGCAACACG GTGGTAACTG CTGCAGTGGT GGAGACCCAG 
AAGAGCACCA AGCTGAAGAG CACACTCAAA CTGGACTTCA AGTTTGACTC CATGACCCTG 
GTCCTGTACA GCCCCAATGG GACTGAGGTG TTGGACTCGA GGGATGAGCA GCTGAAGCTG 
GCTGAGTTTA CCCTGGGGAC TATCTCTACA TCTGTCCACA TGTTCTCAGA CGGATCTATG 
AAGGCCTCCG TCAAACTCAC AGACTGCCTG CTAGACGACA AAAGATGCAG GGTCAAGAAG 
ATCACTGCCA GGATGATGGC GATGCGTCCG GGCGCTGAGA GGAACGTGAT GGTGGAGGTG 
AACTACAAGC AGGGCCGCGA CGGCACCTCT TTGGACACGG TGGTGCAGGA CGTGTACCTC 
TGTGCCAGCA TGGAGTTCCT GCTCACTGTG GCCGACATCT TCCTCAAAGC CAGCCAGCAG 
GGCTTCTCCT CCGCCACCCC CAAGAACAGC AGTAGCACTA AACCCACTGC AGCCGCAGCC 
TCCAAGGAAT CTGCTCCAGT GGGGGTGGTG GCAAAGTCAG AGATTAATGT TCTGGTGAAG 
AACCCCGAGA TTGTGTTTGT GGCCGACCTG ACACGTGCCG ACGCCCCGGC CCTGGTCATG 
ACCACGGAGT GTGAGCTGGT GATGAAGAGC GACCTGGACG GTCAGCTGAT GACTGCGGTG 
GTCAAGGACC TGAAGGTGGT GGCCTGCCCC TTCCTGAAGG AGAAGAGAAA CAAGGAGACC 
ACTGTGCTGC AGCCCTGCCA AGTGTTCTTC AAGAGCACAC AGTCCTCCAC CTCCCCGCAG 
GCCATGGAGG TCTGCATCAA CGCACTCACA CTCAAGGTGT CGCCCATCAT TATCAACACA 
GTGATTACCA TTCAGTCGGT GCTGAGCCCG GCCGGTGCCG AGACACCCCA GTCTGAGCTA 
GAGAGCCCCG TGCCCCCAGA CCTGTGGGAG CGCCGGTCCT GGAGGGACCT CAAGCTGTGG 
TTCCTGGAGG AGGAGGGTAA AGACGAGGAT GCCCAGTCCC TGGTCCCGCT GATTCCCCAG 
GGCGAGTCCC TGCAGTTGGC CATCAACTCC ATCTGCCTGA CACTGGAGGC AGGGGTGGGC 
CACCGCACCG TCCCCATGCT CCTGGCCAAG TCCTCCTTCC ACGGTGACGT CAAGAACTGG 
AGCACCCTCA TCAACCTGCA CAGCCAGCTC AACCTGGAGG TTCACTACTT CAACGAGGTG 
ATGGGGGTGT GGGAGCCACT GCTAGAGCCC CTAGAAGACG AGACCCGAGA TGGATTCAGA 
CCCTGGACCC TGGGATTAAA GATGAAGAAG AAGCCAGTGA AGTACACAGG TATTTCAGAA 
GAAGTGGACT ACAGCATCCC TGACTACAAG ACTGTCATCA GCATCAGCAG CAAGGACCAA 
CTTAATATCA CCCTCTCCAA GTGTGGACTG GGCATGCTCA GTAACCTGGG CACGGCCTTT 
GCGGAGGCGG CCAAGCAGGC ATCGGATAGT TTCCAGAAGG ACGAGGCTCC GTTCGTCGTG 
AAGAACCGTC TAGGTCTGAC TGTGTCGGTG GTGCACAGTG AGCTGTTCAG CCCCCTGGGT 
CGCGAGGCCA CGGCGCGCCG GGTAGAGCTG CAGGACGGAG AGAGCCTCAA CATGGACTAC 
CGTCGCACCA CCACCCACTC AGACCAGTTC TCTGCCATGA CTTCATTGGT GGCCAAGGAC 
TACTACATAC AGCCCACTCC GGTGGGCCAC ACTTCAACAG GCATGATCCC GTTGATCAAA 
GTGGGGCGTG GCATGTACAG TGTCATGCAC AAAGACTCTG GGGTCACCCG TTTCCTGGTC 
TGTCAGATCT ACTCTGTGGA GGGAAGCAAG TACATCAAGA TCCGCTCTCC CTTCCAGATC 
ATAAACCACT TCTCCATCCC ATTCAGAGTG ATGGAGGGCT CTACCAGTTT GGGGATATCA 
CTGCCTACAG AGGAGTTCTG TGTGCCTCTG GACTCCTACA GGTCAGAGCT AAGACTGCAG 
CCGGTAACCG AGGGAGACGA TCAGTACGAC TGCTCCGAGG GTTTCAGCTA CGAGTTGGTG 
AGCCAGCAGC CCGGTGCACG TGTGCAGCAG ACCTGTCATC GCCATGGTGA CAGTGCTGGC 
GTCCTCATGG TCAACATCGT CCCTCTGAAA GACACGGTGA CATCACAACA CACAGGAATG 
GCTGGGGAGC ACTTTGACGA GCCCTACATC CTGCACCTGT GGCCCTCCGT CCTGCTTCGG 
AACCTTCTGC CTTACCCCAT CACCTATCGC CTACAGGACA GTGAAGAGCC CACCCCCATC 
ACCCTGATGG ACGGCCACTC GGCCCAGCTG CACACCGCCA TCATGGACCA GTCCAGCCTG 
GAACTCCGCC TCCTCGACTA CCTGGGTCAG GATTGGCAGG CCCACTACGG TCTCTGTGGC 
GACCTGGATG AGATCACCTT CATTGTGTTC AGCTCTCTGC GCGATGAAGA GGAGGTGGTC 
AGGTTAGGAG AGAGGGGAGA AGGGAAGAGG GCAGAGCTCG ATATTGCGGT GCATGTGAAG 
TACGACGCGG GCCAGACTGA GGTGGCTATC CATTCTCCCT ACTGGATGGT GAACAAGACG 
GGCCGCCTGC TGCAATACAA GGCCGATGAC ATCCACCGCA AACACGCCAT GGACTACGAT 
ATGCCTCTGC TCTTCTCCTT CAAGCCACAC AACTTTCTTC AGAACAACAA GGTGCGGCTG 
ATGATCTCGG ACAGCGACCT GTCGGATGAT TTCTCCCTGG ATACCGTGGG TAGTTATGGT 
GAAGTGAAGT GCAAAGGGAG GCTGAAAGAT TACGTGATCG GAGTGAAGAT CGACGCCAGC 
AGTTTCAGCC TGACCCGTAT CGTAAGCTTT GTGCCATACT ACCTGTTAGT GAACAGGACC 
AAACATGTGA TCTGGGTGGG AGAGGAGGGC CAAGACGACT GGACCGAGGC CAGACCACAA 
CAGGCAGCCA TCCCGTTTTG GCCTGAGAGG GACTCCAAGA AGCTCAAAGT CAAAGTAGAA 
GGCAGTCGGT CAACTCCTCA AACCATGGAC TTCACCAAAC CGGAAAACTG CCTGCTGCTG 
CATCTGGACA ACACTCTGGG CGGGGTCATT ATGGATGTGA ACCTATCGGA CCACTCGATT 
ATCATCCGTT TCTCTGACTA CCACGACGGG GCCGCCCCCT TCCTCATCAT CAACCACACC 
CAGGAGCAGA CGGTGGAGTT CTACCAGAGA TTTCGGAGAG ATTCATCAGA ATGGGTGGCA 
GACGAGCTGC TCGGCCTCTC CTTCTCAGTA GAGGAGACCC AGCCCAGTAG TCAGAAGGAG 
AAGGACAAGC TAAGCCCCGG GAAGGCAATG TACTACACCT GGGCTGAGCC CACTGGATCG 
CGCTCTCTGG CCTGGCAGTG TGGCAGCTAC AGCGGCGAGC TGAGGAGCGA AGAGGACCTG 
CGGTTGGACG TGAACGAGGA GGGTAAGCTG TACCTCATCT CGTTCTACGA GGGCCTCCAG 
CGGGTGGCGC TGTTCACAGA GGACAGACGC ATATACAAGC TGCTGTGTGA GAGTGAGAAG 
GCCGAGCTGG CCGAACAAGA GATCATCCTC TCCCTGCAAG ACGTGGGCGT CTCCCTGGTC 
AACAACAGCA GCCGCCAGGA GGTGTCCTTC ATCGGTATTA CCAGTTCAGA CGTGGTGTGG 
GAGTCCAAGC CAAAGAAGAA GTCTCGCTGG AAACCCCTGA GTGGAAAAGA TGCCGAGCTG 
CTGGAGACCC ACTTCAAAGA GTACACAGAG AGTGGACCAG TGGACAACGC CATCTTAGAC 
CTGGAGAACA ACTTCCAGGT GTCTTTGACC CATAATGGGC AAGACATGAG GATGATGCAG 
CCATATGACG CTCCAATCAG GAGGAGCTTC CTGCCAGGGG TGAAGGTGGA GTACAGCGTA 
TCGGCCCGCC AGAGTGCCTA CCGTGTCCAG ATCAACCGCA TACAGATCCA GAATCAGCTA 
CCTGGAGCAA TCTTCCCATA TGTCTTCTAC CCTATAAAAC TACCTAAGTC TGTCACCATG 
GACTCAGAGC CCAAACCTCT GACCGACATC AGCATTGTTA CCAGGGCAGC AGGGCACTCT 
GATATCTCAC GCATCAAGTA TTTCAAGGTG CTAATCCAGG AGATGGATCT GAAGTTGGAC 
TTGGGCTTCC TTTATGCCAT CTTGGATCTG TTCACTCCGG AGAACGCCAG CGTGATGACG 
TCAGAGCAGC AGGTGGAGTT GTTTGAGAAG GACATAGAGT ATCTGAAGAC GGAGCTCAAC 
GACGTCTCGG CCACTGACAC ATCCCCTATC AGTCTCTATG AGTACTTCCA CATCTCTCCT 
ATCAAGCTGC ACCTCAGTTT CTCTCTGAGT TCTGGAGGAG AGGATGGTAA CAAGGAGAAG 
AGGGAGACCG AGATCATCCC TGTCCAGTCC CTCAATCTGC TGCTGAAAAG CATCGGAGCA 
ACCCTCACTG ACGTGCAGGA TGTCGTCTTC AAATTGGCCT TCTTCGAGCT GACCTTCCAG 
TTTCGCACTC GCCAGCAACT ACAATGGGAG GTCATCAGAC ACTACTCGAA ACAGGCGATA 
AAGCAGATGT ATGTGCTGGT GTTGGGCTTG GACGTGCTGG GAAACCCCTT TGGACTGATC 
CGAGGTCTGT CGGAGGGAGT GGAGGCTTTC TTCTACGAGC CCTATCAGGG AGCTATCCAG 
GGGCCAGAGG AGTTTGTGGA GGGTATGGCG CTGGGGGTGA AAGCGCTGGT TGGAGGAGCA 
GTAGGTGGCA TTGCGGGCGC TGCCTCCAGG ATCACAGGCG CCATGGCGAA GGGTGTGGCT 
GCCATGACAA TGGACGAGGA GTACCAGCAG AAGAGACGGG AGGCTATGAA CAAGCAGCCT 
AGCGGCCTGA GAGAGGGCCT GACCCGCGGA GGCAAAGGAC TGGTGTCTGG CTTTGTCAGT 
GGGATCACAG GGATTGTCAC CAAGCCCATT AAAGGTGCTC AGAAGGAGGG CGCGGCGGGC 
TTCTTTAAGG GTGTGGGCAA GGGGCTAGTG GGTGCTGTGG CCAGACCCAC TGGAGGCATC 
ATCGACATGG CCAGCAGCAC CTTCCAGGGC ATCAAGAGGG CAGCTGAGAC ATCCCAGGAT 
GTGGAGAGTC TGCGTCCGCC TCGCTTCATC CATGAGGACG GGGTCATCCG ACCATACAAG 
GAAAGAGAGG GCATCGGCAG CCAGATGCTA CAGAAAATTG AAAACGGAAG GTTCGCCAAG 
TACAGATACT TTGCGCACGC CAAAGTGAAC GAGTCCGATT TCCTGATGAT CACCAAAAGG 
GGAATCTTTT TCGTGACCAA AGGTACATTT GGGCAGCTGA CCTGCGAGTG GCAGTATCTG 
TTCGACGAGT TCACCAAGGA GCCCATGATC GTGGAGGGGA GGCGGCTTCG GATCGAGGCC 
AAGGAGAGAG TGAAGTCTGT GTTTCATGCC AAAGAGTTTG GGAAGATAAT CAACTTCAGA 
AGTCCAGAAA TAGCCAAGTG GGTTCTTACC AAGCTTGAAG ACGCCAGGGA GAGTCTACAC 
AAATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps13a ( XM_021621037.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021476712.1 Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X3, mRNA.

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CloneID	OOf56505
Clone ID Related Accession (Same CDS sequence)	XM_021621037.1
Accession Version	XM_021621037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	vacuolar protein sorting-associated protein 13A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGC 
AACACGGTGG TAACTGCTGC AGTGGTGGAG ACCCAGAAGA GCACCAAGCT GAAGAGCACA 
CTCAAACTGG ACTTCAAGTT TGACTCCATG ACCCTGGTCC TGTACAGCCC CAATGGGACT 
GAGGTGCAGG TGTTGGACTC GAGGGATGAG CAGCTGAAGC TGGCTGAGTT TACCCTGGGG 
ACTATCTCTA CATCTGTCCA CATGTTCTCA GACGGATCTA TGAAGGCCTC CGTCAAACTC 
ACAGACTGCC TGCTAGACGA CAAAAGATGC AGGGTCAAGA AGATCACTGC CAGGATGATG 
GCGATGCGTC CGGGCGCTGA GAGGAACGTG ATGGTGGAGG TGAACTACAA GCAGGGCCGC 
GACGGCACCT CTTTGGACAC GGTGGTGCAG GACGTGTACC TCTGTGCCAG CATGGAGTTC 
CTGCTCACTG TGGCCGACAT CTTCCTCAAA GCCAGCCAGC AGGGCTTCTC CTCCGCCACC 
CCCAAGAACA GCAGTAGCAC TAAACCCACT GCAGCCGCAG CCTCCAAGGA ATCTGCTCCA 
GTGGGGGTGG TGGCAAAGTC AGAGATTAAT GTTCTGGTGA AGAACCCCGA GATTGTGTTT 
GTGGCCGACC TGACACGTGC CGACGCCCCG GCCCTGGTCA TGACCACGGA GTGTGAGCTG 
GTGATGAAGA GCGACCTGGA CGGTCAGCTG ATGACTGCGG TGGTCAAGGA CCTGAAGGTG 
GTGGCCTGCC CCTTCCTGAA GGAGAAGAGA AACAAGGAGA CCACTGTGCT GCAGCCCTGC 
CAAGTGTTCT TCAAGAGCAC ACAGTCCTCC ACCTCCCCGC AGGCCATGGA GGTCTGCATC 
AACGCACTCA CACTCAAGGT GTCGCCCATC ATTATCAACA CAGTGATTAC CATTCAGTCG 
GTGCTGAGCC CGGCCGGTGC CGAGACACCC CAGTCTGAGC TAGAGAGCCC CGTGCCCCCA 
GACCTGTGGG AGCGCCGGTC CTGGAGGGAC CTCAAGCTGT GGTTCCTGGA GGAGGAGGGT 
AAAGACGAGG ATGCCCAGTC CCTGGTCCCG CTGATTCCCC AGGGCGAGTC CCTGCAGTTG 
GCCATCAACT CCATCTGCCT GACACTGGAG GCAGGGGTGG GCCACCGCAC CGTCCCCATG 
CTCCTGGCCA AGTCCTCCTT CCACGGTGAC GTCAAGAACT GGAGCACCCT CATCAACCTG 
CACAGCCAGC TCAACCTGGA GGTTCACTAC TTCAACGAGG TGATGGGGGT GTGGGAGCCA 
CTGCTAGAGC CCCTAGAAGA CGAGACCCGA GATGGATTCA GACCCTGGAC CCTGGGATTA 
AAGATGAAGA AGAAGCCAGT GAAGTACACA GGTATTTCAG AAGAAGTGGA CTACAGCATC 
CCTGACTACA AGACTGTCAT CAGCATCAGC AGCAAGGACC AACTTAATAT CACCCTCTCC 
AAGTGTGGAC TGGGCATGCT CAGTAACCTG GGCACGGCCT TTGCGGAGGC GGCCAAGCAG 
GCATCGGATA GTTTCCAGAA GGACGAGGCT CCGTTCGTCG TGAAGAACCG TCTAGGTCTG 
ACTGTGTCGG TGGTGCACAG TGAGCTGTTC AGCCCCCTGG GTCGCGAGGC CACGGCGCGC 
CGGGTAGAGC TGCAGGACGG AGAGAGCCTC AACATGGACT ACCGTCGCAC CACCACCCAC 
TCAGACCAGT TCTCTGCCAT GACTTCATTG GTGGCCAAGG ACTACTACAT ACAGCCCACT 
CCGGTGGGCC ACACTTCAAC AGGCATGATC CCGTTGATCA AAGTGGGGCG TGGCATGTAC 
AGTGTCATGC ACAAAGACTC TGGGGTCACC CGTTTCCTGG TCTGTCAGAT CTACTCTGTG 
GAGGGAAGCA AGTACATCAA GATCCGCTCT CCCTTCCAGA TCATAAACCA CTTCTCCATC 
CCATTCAGAG TGATGGAGGG CTCTACCAGT TTGGGGATAT CACTGCCTAC AGAGGAGTTC 
TGTGTGCCTC TGGACTCCTA CAGGTCAGAG CTAAGACTGC AGCCGGTAAC CGAGGGAGAC 
GATCAGTACG ACTGCTCCGA GGGTTTCAGC TACGAGTTGG TGAGCCAGCA GCCCGGTGCA 
CGTGTGCAGC AGACCTGTCA TCGCCATGGT GACAGTGCTG GCGTCCTCAT GGTCAACATC 
GTCCCTCTGA AAGACACGGT GACATCACAA CACACAGGAA TGGCTGGGGA GCACTTTGAC 
GAGCCCTACA TCCTGCACCT GTGGCCCTCC GTCCTGCTTC GGAACCTTCT GCCTTACCCC 
ATCACCTATC GCCTACAGGA CAGTGAAGAG CCCACCCCCA TCACCCTGAT GGACGGCCAC 
TCGGCCCAGC TGCACACCGC CATCATGGAC CAGTCCAGCC TGGAACTCCG CCTCCTCGAC 
TACCTGGGTC AGGATTGGCA GGCCCACTAC GGTCTCTGTG GCGACCTGGA TGAGATCACC 
TTCATTGTGT TCAGCTCTCT GCGCGATGAA GAGGAGGTGG TCAGGTTAGG AGAGAGGGGA 
GAAGGGAAGA GGGCAGAGCT CGATATTGCG GTGCATGTGA AGTACGACGC GGGCCAGACT 
GAGGTGGCTA TCCATTCTCC CTACTGGATG GTGAACAAGA CGGGCCGCCT GCTGCAATAC 
AAGGCCGATG ACATCCACCG CAAACACGCC ATGGACTACG ATATGCCTCT GCTCTTCTCC 
TTCAAGCCAC ACAACTTTCT TCAGAACAAC AAGGTGCGGC TGATGATCTC GGACAGCGAC 
CTGTCGGATG ATTTCTCCCT GGATACCGTG GGTAGTTATG GTGAAGTGAA GTGCAAAGGG 
AGGCTGAAAG ATTACGTGAT CGGAGTGAAG ATCGACGCCA GCAGTTTCAG CCTGACCCGT 
ATCGTAAGCT TTGTGCCATA CTACCTGTTA GTGAACAGGA CCAAACATGT GATCTGGGTG 
GGAGAGGAGG GCCAAGACGA CTGGACCGAG GCCAGACCAC AACAGGCAGC CATCCCGTTT 
TGGCCTGAGA GGGACTCCAA GAAGCTCAAA GTCAAAGTAG AAGGCAGTCG GTCAACTCCT 
CAAACCATGG ACTTCACCAA ACCGGAAAAC TGCCTGCTGC TGCATCTGGA CAACACTCTG 
GGCGGGGTCA TTATGGATGT GAACCTATCG GACCACTCGA TTATCATCCG TTTCTCTGAC 
TACCACGACG GGGCCGCCCC CTTCCTCATC ATCAACCACA CCCAGGAGCA GACGGTGGAG 
TTCTACCAGA GATTTCGGAG AGATTCATCA GAATGGGTGG CAGACGAGCT GCTCGGCCTC 
TCCTTCTCAG TAGAGGAGAC CCAGCCCAGT AGTCAGAAGG AGAAGGACAA GCTAAGCCCC 
GGGAAGGCAA TGTACTACAC CTGGGCTGAG CCCACTGGAT CGCGCTCTCT GGCCTGGCAG 
TGTGGCAGCT ACAGCGGCGA GCTGAGGAGC GAAGAGGACC TGCGGTTGGA CGTGAACGAG 
GAGGGTAAGC TGTACCTCAT CTCGTTCTAC GAGGGCCTCC AGCGGGTGGC GCTGTTCACA 
GAGGACAGAC GCATATACAA GCTGCTGTGT GAGAGTGAGA AGGCCGAGCT GGCCGAACAA 
GAGATCATCC TCTCCCTGCA AGACGTGGGC GTCTCCCTGG TCAACAACAG CAGCCGCCAG 
GAGGTGTCCT TCATCGGTAT TACCAGTTCA GACGTGGTGT GGGAGTCCAA GCCAAAGAAG 
AAGTCTCGCT GGAAACCCCT GAGTGGAAAA GATGCCGAGC TGCTGGAGAC CCACTTCAAA 
GAGTACACAG AGAGTGGACC AGTGGACAAC GCCATCTTAG ACCTGGAGAA CAACTTCCAG 
GTGTCTTTGA CCCATAATGG GCAAGACATG AGGATGATGC AGCCATATGA CGCTCCAATC 
AGGAGGAGCT TCCTGCCAGG GGTGAAGGTG GAGTACAGCG TATCGGCCCG CCAGAGTGCC 
TACCGTGTCC AGATCAACCG CATACAGATC CAGAATCAGC TACCTGGAGC AATCTTCCCA 
TATGTCTTCT ACCCTATAAA ACTACCTAAG TCTGTCACCA TGGACTCAGA GCCCAAACCT 
CTGACCGACA TCAGCATTGT TACCAGGGCA GCAGGGCACT CTGATATCTC ACGCATCAAG 
TATTTCAAGG TGCTAATCCA GGAGATGGAT CTGAAGTTGG ACTTGGGCTT CCTTTATGCC 
ATCTTGGATC TGTTCACTCC GGAGAACGCC AGCGTGATGA CGTCAGAGCA GCAGGTGGAG 
TTGTTTGAGA AGGACATAGA GTATCTGAAG ACGGAGCTCA ACGACGTCTC GGCCACTGAC 
ACATCCCCTA TCAGTCTCTA TGAGTACTTC CACATCTCTC CTATCAAGCT GCACCTCAGT 
TTCTCTCTGA GTTCTGGAGG AGAGGATGGT AACAAGGAGA AGAGGGAGAC CGAGATCATC 
CCTGTCCAGT CCCTCAATCT GCTGCTGAAA AGCATCGGAG CAACCCTCAC TGACGTGCAG 
GATGTCGTCT TCAAATTGGC CTTCTTCGAG CTGACCTTCC AGTTTCGCAC TCGCCAGCAA 
CTACAATGGG AGGTCATCAG ACACTACTCG AAACAGGCGA TAAAGCAGAT GTATGTGCTG 
GTGTTGGGCT TGGACGTGCT GGGAAACCCC TTTGGACTGA TCCGAGGTCT GTCGGAGGGA 
GTGGAGGCTT TCTTCTACGA GCCCTATCAG GGAGCTATCC AGGGGCCAGA GGAGTTTGTG 
GAGGGTATGG CGCTGGGGGT GAAAGCGCTG GTTGGAGGAG CAGTAGGTGG CATTGCGGGC 
GCTGCCTCCA GGATCACAGG CGCCATGGCG AAGGGTGTGG CTGCCATGAC AATGGACGAG 
GAGTACCAGC AGAAGAGACG GGAGGCTATG AACAAGCAGC CTAGCGGCCT GAGAGAGGGC 
CTGACCCGCG GAGGCAAAGG ACTGGTGTCT GGCTTTGTCA GTGGGATCAC AGGGATTGTC 
ACCAAGCCCA TTAAAGGTGC TCAGAAGGAG GGCGCGGCGG GCTTCTTTAA GGGTGTGGGC 
AAGGGGCTAG TGGGTGCTGT GGCCAGACCC ACTGGAGGCA TCATCGACAT GGCCAGCAGC 
ACCTTCCAGG GCATCAAGAG GGCAGCTGAG ACATCCCAGG ATGTGGAGAG TCTGCGTCCG 
CCTCGCTTCA TCCATGAGGA CGGGGTCATC CGACCATACA AGGAAAGAGA GGGCATCGGC 
AGCCAGATGC TACAGAAAAT TGAAAACGGA AGGTTCGCCA AGTACAGATA CTTTGCGCAC 
GCCAAAGTGA ACGAGTCCGA TTTCCTGATG ATCACCAAAA GGGGAATCTT TTTCGTGACC 
AAAGGTACAT TTGGGCAGCT GACCTGCGAG TGGCAGTATC TGTTCGACGA GTTCACCAAG 
GAGCCCATGA TCGTGGAGGG GAGGCGGCTT CGGATCGAGG CCAAGGAGAG AGTGAAGTCT 
GTGTTTCATG CCAAAGAGTT TGGGAAGATA ATCAACTTCA GAAGTCCAGA AATAGCCAAG 
TGGGTTCTTA CCAAGCTTGA AGACGCCAGG GAGAGTCTAC ACAAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021476712.1
CDS	274..10044
Translation	MVFESVVVDVLNRFLGDYVVNLDSSQLKLGIWGGDAVLRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDGVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEETKQKAAEQENPALEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPNCPFSFGVSLQNLSLQTADENWMPCLLDEKAKLFFKLVQLDNLFAYWNVNSMLYSNHMPDEALRCLQDSIPGGSTPMNHDFIFRPITADAKLRMNPRSDVDFSSPKVDLVVNLREVAVELNRPQYISILELLGSVDMMSRNLPYRKYRPQVHVHTNAYLWWQYVITGIMEVDVKPRLHMWSWQHIRCHRQTVKQYRELYKAKITSKKPTEKQLRELEEPERTLDIFNITLARQQAEMEASKAGLRIFRPGVKVEEEESQGWLGWMWSGWSGDCGAEAKEVKTGAFDELMTSAEKAKLYTAIGYSEIAVNHNLPKNFEDMKINFHMERMSVSMKDNKDQNEILKLTVEDLKSMLTQRPGAQAIKLSAQLSLFEVTGLASNRPAPTLLSSRKAATVTGTPLLDILFETNPLDESADQRLRVESQPLEIIYDAVTVNSMSAFFSPPDDLQLDDLTNATLMKLEQFRDRTSTGLMYVIETQKVLDLNVNLMASYVIVPQTGFHDSTQNLMLLDLGHFKVSSLSREGLPQLSVGRSTIEDIMSRAYDSFDVQLSSLQFLYSKPDGDWKKARKLRQSALHILEPVDVKVVFSRAMVVTDSRMPKFKISGELPLLSLRISDDKVRGVLELIDSIPLPESRPAPRSTNAHSTSKALKQSYTPQLTPRKPTMAELRNSLIWESGSEEDLFYDAPSSPIGDRPFFPDNMPSPLQRSDSVKRRGLVKKDPKKNMTEFLMTFEISKLSVQLCRLSGGEEVTVLHLDIEGLGTELKLRTFDMTSYTFLREICLECPEYMDSEEKKVHLITTLDNTEDDLLTLEYTKADKNGPEFKSEYKNTEQSVKVNFSSLDIHLHTEALLNAMNFLISLLPPTKEVAPSQEELPTIPEEDEGEGEEGEKKEGSAVAKKTSSKKSKFEDVVNLHIRADLNCLKVFIRGQSHRIAEIRIEGLVSEVLMRKKAVEVLANLKNIVILDCDKEALYKQCVSIADEEVFAFRMVNHTEATEGDAYLDMGKVDTSVTLTVGCIQVIFLNKFVSSILAFINNFQVAKEALAEVTVQAAEKAATGVMELAERSTRISLDVDFNAPVVFLPQSSSSANVIVADLGMVTVKNRFAMVPSQMHVKIPPVMDTMTVGLSDLKMYRTTFEEGRFQGEIQLLKPVNLDLSIQRNLSASWYHSIPDIEITAHLKPMNLILGQDDMTVVLRTLSGNLSETSDSASPPPPPRTVDRSDTASTKGSQASGGNTVVTAAVVETQKSTKLKSTLKLDFKFDSMTLVLYSPNGTEVQVLDSRDEQLKLAEFTLGTISTSVHMFSDGSMKASVKLTDCLLDDKRCRVKKITARMMAMRPGAERNVMVEVNYKQGRDGTSLDTVVQDVYLCASMEFLLTVADIFLKASQQGFSSATPKNSSSTKPTAAAASKESAPVGVVAKSEINVLVKNPEIVFVADLTRADAPALVMTTECELVMKSDLDGQLMTAVVKDLKVVACPFLKEKRNKETTVLQPCQVFFKSTQSSTSPQAMEVCINALTLKVSPIIINTVITIQSVLSPAGAETPQSELESPVPPDLWERRSWRDLKLWFLEEEGKDEDAQSLVPLIPQGESLQLAINSICLTLEAGVGHRTVPMLLAKSSFHGDVKNWSTLINLHSQLNLEVHYFNEVMGVWEPLLEPLEDETRDGFRPWTLGLKMKKKPVKYTGISEEVDYSIPDYKTVISISSKDQLNITLSKCGLGMLSNLGTAFAEAAKQASDSFQKDEAPFVVKNRLGLTVSVVHSELFSPLGREATARRVELQDGESLNMDYRRTTTHSDQFSAMTSLVAKDYYIQPTPVGHTSTGMIPLIKVGRGMYSVMHKDSGVTRFLVCQIYSVEGSKYIKIRSPFQIINHFSIPFRVMEGSTSLGISLPTEEFCVPLDSYRSELRLQPVTEGDDQYDCSEGFSYELVSQQPGARVQQTCHRHGDSAGVLMVNIVPLKDTVTSQHTGMAGEHFDEPYILHLWPSVLLRNLLPYPITYRLQDSEEPTPITLMDGHSAQLHTAIMDQSSLELRLLDYLGQDWQAHYGLCGDLDEITFIVFSSLRDEEEVVRLGERGEGKRAELDIAVHVKYDAGQTEVAIHSPYWMVNKTGRLLQYKADDIHRKHAMDYDMPLLFSFKPHNFLQNNKVRLMISDSDLSDDFSLDTVGSYGEVKCKGRLKDYVIGVKIDASSFSLTRIVSFVPYYLLVNRTKHVIWVGEEGQDDWTEARPQQAAIPFWPERDSKKLKVKVEGSRSTPQTMDFTKPENCLLLHLDNTLGGVIMDVNLSDHSIIIRFSDYHDGAAPFLIINHTQEQTVEFYQRFRRDSSEWVADELLGLSFSVEETQPSSQKEKDKLSPGKAMYYTWAEPTGSRSLAWQCGSYSGELRSEEDLRLDVNEEGKLYLISFYEGLQRVALFTEDRRIYKLLCESEKAELAEQEIILSLQDVGVSLVNNSSRQEVSFIGITSSDVVWESKPKKKSRWKPLSGKDAELLETHFKEYTESGPVDNAILDLENNFQVSLTHNGQDMRMMQPYDAPIRRSFLPGVKVEYSVSARQSAYRVQINRIQIQNQLPGAIFPYVFYPIKLPKSVTMDSEPKPLTDISIVTRAAGHSDISRIKYFKVLIQEMDLKLDLGFLYAILDLFTPENASVMTSEQQVELFEKDIEYLKTELNDVSATDTSPISLYEYFHISPIKLHLSFSLSSGGEDGNKEKRETEIIPVQSLNLLLKSIGATLTDVQDVVFKLAFFELTFQFRTRQQLQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGIAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRAAETSQDVESLRPPRFIHEDGVIRPYKEREGIGSQMLQKIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKGTFGQLTCEWQYLFDEFTKEPMIVEGRRLRIEAKERVKSVFHAKEFGKIINFRSPEIAKWVLTKLEDARESLHKY

Target ORF information:

RefSeq Version	XM_021621037.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621037.1

ORF Insert Sequence:

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGC 
AACACGGTGG TAACTGCTGC AGTGGTGGAG ACCCAGAAGA GCACCAAGCT GAAGAGCACA 
CTCAAACTGG ACTTCAAGTT TGACTCCATG ACCCTGGTCC TGTACAGCCC CAATGGGACT 
GAGGTGCAGG TGTTGGACTC GAGGGATGAG CAGCTGAAGC TGGCTGAGTT TACCCTGGGG 
ACTATCTCTA CATCTGTCCA CATGTTCTCA GACGGATCTA TGAAGGCCTC CGTCAAACTC 
ACAGACTGCC TGCTAGACGA CAAAAGATGC AGGGTCAAGA AGATCACTGC CAGGATGATG 
GCGATGCGTC CGGGCGCTGA GAGGAACGTG ATGGTGGAGG TGAACTACAA GCAGGGCCGC 
GACGGCACCT CTTTGGACAC GGTGGTGCAG GACGTGTACC TCTGTGCCAG CATGGAGTTC 
CTGCTCACTG TGGCCGACAT CTTCCTCAAA GCCAGCCAGC AGGGCTTCTC CTCCGCCACC 
CCCAAGAACA GCAGTAGCAC TAAACCCACT GCAGCCGCAG CCTCCAAGGA ATCTGCTCCA 
GTGGGGGTGG TGGCAAAGTC AGAGATTAAT GTTCTGGTGA AGAACCCCGA GATTGTGTTT 
GTGGCCGACC TGACACGTGC CGACGCCCCG GCCCTGGTCA TGACCACGGA GTGTGAGCTG 
GTGATGAAGA GCGACCTGGA CGGTCAGCTG ATGACTGCGG TGGTCAAGGA CCTGAAGGTG 
GTGGCCTGCC CCTTCCTGAA GGAGAAGAGA AACAAGGAGA CCACTGTGCT GCAGCCCTGC 
CAAGTGTTCT TCAAGAGCAC ACAGTCCTCC ACCTCCCCGC AGGCCATGGA GGTCTGCATC 
AACGCACTCA CACTCAAGGT GTCGCCCATC ATTATCAACA CAGTGATTAC CATTCAGTCG 
GTGCTGAGCC CGGCCGGTGC CGAGACACCC CAGTCTGAGC TAGAGAGCCC CGTGCCCCCA 
GACCTGTGGG AGCGCCGGTC CTGGAGGGAC CTCAAGCTGT GGTTCCTGGA GGAGGAGGGT 
AAAGACGAGG ATGCCCAGTC CCTGGTCCCG CTGATTCCCC AGGGCGAGTC CCTGCAGTTG 
GCCATCAACT CCATCTGCCT GACACTGGAG GCAGGGGTGG GCCACCGCAC CGTCCCCATG 
CTCCTGGCCA AGTCCTCCTT CCACGGTGAC GTCAAGAACT GGAGCACCCT CATCAACCTG 
CACAGCCAGC TCAACCTGGA GGTTCACTAC TTCAACGAGG TGATGGGGGT GTGGGAGCCA 
CTGCTAGAGC CCCTAGAAGA CGAGACCCGA GATGGATTCA GACCCTGGAC CCTGGGATTA 
AAGATGAAGA AGAAGCCAGT GAAGTACACA GGTATTTCAG AAGAAGTGGA CTACAGCATC 
CCTGACTACA AGACTGTCAT CAGCATCAGC AGCAAGGACC AACTTAATAT CACCCTCTCC 
AAGTGTGGAC TGGGCATGCT CAGTAACCTG GGCACGGCCT TTGCGGAGGC GGCCAAGCAG 
GCATCGGATA GTTTCCAGAA GGACGAGGCT CCGTTCGTCG TGAAGAACCG TCTAGGTCTG 
ACTGTGTCGG TGGTGCACAG TGAGCTGTTC AGCCCCCTGG GTCGCGAGGC CACGGCGCGC 
CGGGTAGAGC TGCAGGACGG AGAGAGCCTC AACATGGACT ACCGTCGCAC CACCACCCAC 
TCAGACCAGT TCTCTGCCAT GACTTCATTG GTGGCCAAGG ACTACTACAT ACAGCCCACT 
CCGGTGGGCC ACACTTCAAC AGGCATGATC CCGTTGATCA AAGTGGGGCG TGGCATGTAC 
AGTGTCATGC ACAAAGACTC TGGGGTCACC CGTTTCCTGG TCTGTCAGAT CTACTCTGTG 
GAGGGAAGCA AGTACATCAA GATCCGCTCT CCCTTCCAGA TCATAAACCA CTTCTCCATC 
CCATTCAGAG TGATGGAGGG CTCTACCAGT TTGGGGATAT CACTGCCTAC AGAGGAGTTC 
TGTGTGCCTC TGGACTCCTA CAGGTCAGAG CTAAGACTGC AGCCGGTAAC CGAGGGAGAC 
GATCAGTACG ACTGCTCCGA GGGTTTCAGC TACGAGTTGG TGAGCCAGCA GCCCGGTGCA 
CGTGTGCAGC AGACCTGTCA TCGCCATGGT GACAGTGCTG GCGTCCTCAT GGTCAACATC 
GTCCCTCTGA AAGACACGGT GACATCACAA CACACAGGAA TGGCTGGGGA GCACTTTGAC 
GAGCCCTACA TCCTGCACCT GTGGCCCTCC GTCCTGCTTC GGAACCTTCT GCCTTACCCC 
ATCACCTATC GCCTACAGGA CAGTGAAGAG CCCACCCCCA TCACCCTGAT GGACGGCCAC 
TCGGCCCAGC TGCACACCGC CATCATGGAC CAGTCCAGCC TGGAACTCCG CCTCCTCGAC 
TACCTGGGTC AGGATTGGCA GGCCCACTAC GGTCTCTGTG GCGACCTGGA TGAGATCACC 
TTCATTGTGT TCAGCTCTCT GCGCGATGAA GAGGAGGTGG TCAGGTTAGG AGAGAGGGGA 
GAAGGGAAGA GGGCAGAGCT CGATATTGCG GTGCATGTGA AGTACGACGC GGGCCAGACT 
GAGGTGGCTA TCCATTCTCC CTACTGGATG GTGAACAAGA CGGGCCGCCT GCTGCAATAC 
AAGGCCGATG ACATCCACCG CAAACACGCC ATGGACTACG ATATGCCTCT GCTCTTCTCC 
TTCAAGCCAC ACAACTTTCT TCAGAACAAC AAGGTGCGGC TGATGATCTC GGACAGCGAC 
CTGTCGGATG ATTTCTCCCT GGATACCGTG GGTAGTTATG GTGAAGTGAA GTGCAAAGGG 
AGGCTGAAAG ATTACGTGAT CGGAGTGAAG ATCGACGCCA GCAGTTTCAG CCTGACCCGT 
ATCGTAAGCT TTGTGCCATA CTACCTGTTA GTGAACAGGA CCAAACATGT GATCTGGGTG 
GGAGAGGAGG GCCAAGACGA CTGGACCGAG GCCAGACCAC AACAGGCAGC CATCCCGTTT 
TGGCCTGAGA GGGACTCCAA GAAGCTCAAA GTCAAAGTAG AAGGCAGTCG GTCAACTCCT 
CAAACCATGG ACTTCACCAA ACCGGAAAAC TGCCTGCTGC TGCATCTGGA CAACACTCTG 
GGCGGGGTCA TTATGGATGT GAACCTATCG GACCACTCGA TTATCATCCG TTTCTCTGAC 
TACCACGACG GGGCCGCCCC CTTCCTCATC ATCAACCACA CCCAGGAGCA GACGGTGGAG 
TTCTACCAGA GATTTCGGAG AGATTCATCA GAATGGGTGG CAGACGAGCT GCTCGGCCTC 
TCCTTCTCAG TAGAGGAGAC CCAGCCCAGT AGTCAGAAGG AGAAGGACAA GCTAAGCCCC 
GGGAAGGCAA TGTACTACAC CTGGGCTGAG CCCACTGGAT CGCGCTCTCT GGCCTGGCAG 
TGTGGCAGCT ACAGCGGCGA GCTGAGGAGC GAAGAGGACC TGCGGTTGGA CGTGAACGAG 
GAGGGTAAGC TGTACCTCAT CTCGTTCTAC GAGGGCCTCC AGCGGGTGGC GCTGTTCACA 
GAGGACAGAC GCATATACAA GCTGCTGTGT GAGAGTGAGA AGGCCGAGCT GGCCGAACAA 
GAGATCATCC TCTCCCTGCA AGACGTGGGC GTCTCCCTGG TCAACAACAG CAGCCGCCAG 
GAGGTGTCCT TCATCGGTAT TACCAGTTCA GACGTGGTGT GGGAGTCCAA GCCAAAGAAG 
AAGTCTCGCT GGAAACCCCT GAGTGGAAAA GATGCCGAGC TGCTGGAGAC CCACTTCAAA 
GAGTACACAG AGAGTGGACC AGTGGACAAC GCCATCTTAG ACCTGGAGAA CAACTTCCAG 
GTGTCTTTGA CCCATAATGG GCAAGACATG AGGATGATGC AGCCATATGA CGCTCCAATC 
AGGAGGAGCT TCCTGCCAGG GGTGAAGGTG GAGTACAGCG TATCGGCCCG CCAGAGTGCC 
TACCGTGTCC AGATCAACCG CATACAGATC CAGAATCAGC TACCTGGAGC AATCTTCCCA 
TATGTCTTCT ACCCTATAAA ACTACCTAAG TCTGTCACCA TGGACTCAGA GCCCAAACCT 
CTGACCGACA TCAGCATTGT TACCAGGGCA GCAGGGCACT CTGATATCTC ACGCATCAAG 
TATTTCAAGG TGCTAATCCA GGAGATGGAT CTGAAGTTGG ACTTGGGCTT CCTTTATGCC 
ATCTTGGATC TGTTCACTCC GGAGAACGCC AGCGTGATGA CGTCAGAGCA GCAGGTGGAG 
TTGTTTGAGA AGGACATAGA GTATCTGAAG ACGGAGCTCA ACGACGTCTC GGCCACTGAC 
ACATCCCCTA TCAGTCTCTA TGAGTACTTC CACATCTCTC CTATCAAGCT GCACCTCAGT 
TTCTCTCTGA GTTCTGGAGG AGAGGATGGT AACAAGGAGA AGAGGGAGAC CGAGATCATC 
CCTGTCCAGT CCCTCAATCT GCTGCTGAAA AGCATCGGAG CAACCCTCAC TGACGTGCAG 
GATGTCGTCT TCAAATTGGC CTTCTTCGAG CTGACCTTCC AGTTTCGCAC TCGCCAGCAA 
CTACAATGGG AGGTCATCAG ACACTACTCG AAACAGGCGA TAAAGCAGAT GTATGTGCTG 
GTGTTGGGCT TGGACGTGCT GGGAAACCCC TTTGGACTGA TCCGAGGTCT GTCGGAGGGA 
GTGGAGGCTT TCTTCTACGA GCCCTATCAG GGAGCTATCC AGGGGCCAGA GGAGTTTGTG 
GAGGGTATGG CGCTGGGGGT GAAAGCGCTG GTTGGAGGAG CAGTAGGTGG CATTGCGGGC 
GCTGCCTCCA GGATCACAGG CGCCATGGCG AAGGGTGTGG CTGCCATGAC AATGGACGAG 
GAGTACCAGC AGAAGAGACG GGAGGCTATG AACAAGCAGC CTAGCGGCCT GAGAGAGGGC 
CTGACCCGCG GAGGCAAAGG ACTGGTGTCT GGCTTTGTCA GTGGGATCAC AGGGATTGTC 
ACCAAGCCCA TTAAAGGTGC TCAGAAGGAG GGCGCGGCGG GCTTCTTTAA GGGTGTGGGC 
AAGGGGCTAG TGGGTGCTGT GGCCAGACCC ACTGGAGGCA TCATCGACAT GGCCAGCAGC 
ACCTTCCAGG GCATCAAGAG GGCAGCTGAG ACATCCCAGG ATGTGGAGAG TCTGCGTCCG 
CCTCGCTTCA TCCATGAGGA CGGGGTCATC CGACCATACA AGGAAAGAGA GGGCATCGGC 
AGCCAGATGC TACAGAAAAT TGAAAACGGA AGGTTCGCCA AGTACAGATA CTTTGCGCAC 
GCCAAAGTGA ACGAGTCCGA TTTCCTGATG ATCACCAAAA GGGGAATCTT TTTCGTGACC 
AAAGGTACAT TTGGGCAGCT GACCTGCGAG TGGCAGTATC TGTTCGACGA GTTCACCAAG 
GAGCCCATGA TCGTGGAGGG GAGGCGGCTT CGGATCGAGG CCAAGGAGAG AGTGAAGTCT 
GTGTTTCATG CCAAAGAGTT TGGGAAGATA ATCAACTTCA GAAGTCCAGA AATAGCCAAG 
TGGGTTCTTA CCAAGCTTGA AGACGCCAGG GAGAGTCTAC ACAAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps13a ( XM_021621038.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021476713.1 Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X4, mRNA.

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CloneID	OOf56506
Clone ID Related Accession (Same CDS sequence)	XM_021621038.1
Accession Version	XM_021621038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	vacuolar protein sorting-associated protein 13A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGT 
ACTGGTACTA ACCCCACCAG CGGCAACACG GTGGTAACTG CTGCAGTGGT GGAGACCCAG 
AAGAGCACCA AGCTGAAGAG CACACTCAAA CTGGACTTCA AGTTTGACTC CATGACCCTG 
GTCCTGTACA GCCCCAATGG GACTGAGGTG CAGGTGTTGG ACTCGAGGGA TGAGCAGCTG 
AAGCTGGCTG AGTTTACCCT GGGGACTATC TCTACATCTG TCCACATGTT CTCAGACGGA 
TCTATGAAGG CCTCCGTCAA ACTCACAGAC TGCCTGCTAG ACGACAAAAG ATGCAGGGTC 
AAGAAGATCA CTGCCAGGAT GATGGCGATG CGTCCGGGCG CTGAGAGGAA CGTGATGGTG 
GAGGTGAACT ACAAGCAGGG CCGCGACGGC ACCTCTTTGG ACACGGTGGT GCAGGACGTG 
TACCTCTGTG CCAGCATGGA GTTCCTGCTC ACTGTGGCCG ACATCTTCCT CAAAGCCAGC 
CAGCAGGGCT TCTCCTCCGC CACCCCCAAG AACAGCAGTA GCACTAAACC CACTGCAGCC 
GCAGCCTCCA AGGAATCTGC TCCAGTGGGG GTGGTGGCAA AGTCAGAGAT TAATGTTCTG 
GTGAAGAACC CCGAGATTGT GTTTGTGGCC GACCTGACAC GTGCCGACGC CCCGGCCCTG 
GTCATGACCA CGGAGTGTGA GCTGGTGATG AAGAGCGACC TGGACGGTCA GCTGATGACT 
GCGGTGGTCA AGGACCTGAA GGTGGTGGCC TGCCCCTTCC TGAAGGAGAA GAGAAACAAG 
GAGACCACTG TGCTGCAGCC CTGCCAAGTG TTCTTCAAGA GCACACAGTC CTCCACCTCC 
CCGCAGGCCA TGGAGGTCTG CATCAACGCA CTCACACTCA AGGTGTCGCC CATCATTATC 
AACACAGTGA TTACCATTCA GTCGGTGCTG AGCCCGGCCG GTGCCGAGAC ACCCCAGTCT 
GAGCTAGAGA GCCCCGTGCC CCCAGACCTG TGGGAGCGCC GGTCCTGGAG GGACCTCAAG 
CTGTGGTTCC TGGAGGAGGA GGGTAAAGAC GAGGATGCCC AGTCCCTGGT CCCGCTGATT 
CCCCAGGGCG AGTCCCTGCA GTTGGCCATC AACTCCATCT GCCTGACACT GGAGGCAGGG 
GTGGGCCACC GCACCGTCCC CATGCTCCTG GCCAAGTCCT CCTTCCACGG TGACGTCAAG 
AACTGGAGCA CCCTCATCAA CCTGCACAGC CAGCTCAACC TGGAGGTTCA CTACTTCAAC 
GAGGTGATGG GGGTGTGGGA GCCACTGCTA GAGCCCCTAG AAGACGAGAC CCGAGATGGA 
TTCAGACCCT GGACCCTGGG ATTAAAGATG AAGAAGAAGC CAGTGAAGTA CACAGGTATT 
TCAGAAGAAG TGGACTACAG CATCCCTGAC TACAAGACTG TCATCAGCAT CAGCAGCAAG 
GACCAACTTA ATATCACCCT CTCCAAGTGT GGACTGGGCA TGCTCAGTAA CCTGGGCACG 
GCCTTTGCGG AGGCGGCCAA GCAGGCATCG GATAGTTTCC AGAAGGACGA GGCTCCGTTC 
GTCGTGAAGA ACCGTCTAGG TCTGACTGTG TCGGTGGTGC ACAGTGAGCT GTTCAGCCCC 
CTGGGTCGCG AGGCCACGGC GCGCCGGGTA GAGCTGCAGG ACGGAGAGAG CCTCAACATG 
GACTACCGTC GCACCACCAC CCACTCAGAC CAGTTCTCTG CCATGACTTC ATTGGTGGCC 
AAGGACTACT ACATACAGCC CACTCCGGTG GGCCACACTT CAACAGGCAT GATCCCGTTG 
ATCAAAGTGG GGCGTGGCAT GTACAGTGTC ATGCACAAAG ACTCTGGGGT CACCCGTTTC 
CTGGTCTGTC AGATCTACTC TGTGGAGGGA AGCAAGTACA TCAAGATCCG CTCTCCCTTC 
CAGATCATAA ACCACTTCTC CATCCCATTC AGAGTGATGG AGGGCTCTAC CAGTTTGGGG 
ATATCACTGC CTACAGAGGA GTTCTGTGTG CCTCTGGACT CCTACAGGTC AGAGCTAAGA 
CTGCAGCCGG TAACCGAGGG AGACGATCAG TACGACTGCT CCGAGGGTTT CAGCTACGAG 
TTGGTGAGCC AGCAGCCCGG TGCACGTGTG CAGCAGACCT GTCATCGCCA TGGTGACAGT 
GCTGGCGTCC TCATGGTCAA CATCGTCCCT CTGAAAGACA CGGTGACATC ACAACACACA 
GGAATGGCTG GGGAGCACTT TGACGAGCCC TACATCCTGC ACCTGTGGCC CTCCGTCCTG 
CTTCGGAACC TTCTGCCTTA CCCCATCACC TATCGCCTAC AGGACAGTGA AGAGCCCACC 
CCCATCACCC TGATGGACGG CCACTCGGCC CAGCTGCACA CCGCCATCAT GGACCAGTCC 
AGCCTGGAAC TCCGCCTCCT CGACTACCTG GGTCAGGATT GGCAGGCCCA CTACGGTCTC 
TGTGGCGACC TGGATGAGAT CACCTTCATT GTGTTCAGCT CTCTGCGCGA TGAAGAGGAG 
GTGGTCAGGT TAGGAGAGAG GGGAGAAGGG AAGAGGGCAG AGCTCGATAT TGCGGTGCAT 
GTGAAGTACG ACGCGGGCCA GACTGAGGTG GCTATCCATT CTCCCTACTG GATGGTGAAC 
AAGACGGGCC GCCTGCTGCA ATACAAGGCC GATGACATCC ACCGCAAACA CGCCATGGAC 
TACGATATGC CTCTGCTCTT CTCCTTCAAG CCACACAACT TTCTTCAGAA CAACAAGGTG 
CGGCTGATGA TCTCGGACAG CGACCTGTCG GATGATTTCT CCCTGGATAC CGTGGGTAGT 
TATGGTGAAG TGAAGTGCAA AGGGAGGCTG AAAGATTACG TGATCGGAGT GAAGATCGAC 
GCCAGCAGTT TCAGCCTGAC CCGTATCGTA AGCTTTGTGC CATACTACCT GTTAGTGAAC 
AGGACCAAAC ATGTGATCTG GGTGGGAGAG GAGGGCCAAG ACGACTGGAC CGAGGCCAGA 
CCACAACAGG CAGCCATCCC GTTTTGGCCT GAGAGGGACT CCAAGAAGCT CAAAGTCAAA 
GTAGAAGGCA GTCGGTCAAC TCCTCAAACC ATGGACTTCA CCAAACCGGA AAACTGCCTG 
CTGCTGCATC TGGACAACAC TCTGGGCGGG GTCATTATGG ATGTGAACCT ATCGGACCAC 
TCGATTATCA TCCGTTTCTC TGACTACCAC GACGGGGCCG CCCCCTTCCT CATCATCAAC 
CACACCCAGG AGCAGACGGT GGAGTTCTAC CAGAGTAGTC AGAAGGAGAA GGACAAGCTA 
AGCCCCGGGA AGGCAATGTA CTACACCTGG GCTGAGCCCA CTGGATCGCG CTCTCTGGCC 
TGGCAGTGTG GCAGCTACAG CGGCGAGCTG AGGAGCGAAG AGGACCTGCG GTTGGACGTG 
AACGAGGAGG GTAAGCTGTA CCTCATCTCG TTCTACGAGG GCCTCCAGCG GGTGGCGCTG 
TTCACAGAGG ACAGACGCAT ATACAAGCTG CTGTGTGAGA GTGAGAAGGC CGAGCTGGCC 
GAACAAGAGA TCATCCTCTC CCTGCAAGAC GTGGGCGTCT CCCTGGTCAA CAACAGCAGC 
CGCCAGGAGG TGTCCTTCAT CGGTATTACC AGTTCAGACG TGGTGTGGGA GTCCAAGCCA 
AAGAAGAAGT CTCGCTGGAA ACCCCTGAGT GGAAAAGATG CCGAGCTGCT GGAGACCCAC 
TTCAAAGAGT ACACAGAGAG TGGACCAGTG GACAACGCCA TCTTAGACCT GGAGAACAAC 
TTCCAGGTGT CTTTGACCCA TAATGGGCAA GACATGAGGA TGATGCAGCC ATATGACGCT 
CCAATCAGGA GGAGCTTCCT GCCAGGGGTG AAGGTGGAGT ACAGCGTATC GGCCCGCCAG 
AGTGCCTACC GTGTCCAGAT CAACCGCATA CAGATCCAGA ATCAGCTACC TGGAGCAATC 
TTCCCATATG TCTTCTACCC TATAAAACTA CCTAAGTCTG TCACCATGGA CTCAGAGCCC 
AAACCTCTGA CCGACATCAG CATTGTTACC AGGGCAGCAG GGCACTCTGA TATCTCACGC 
ATCAAGTATT TCAAGGTGCT AATCCAGGAG ATGGATCTGA AGTTGGACTT GGGCTTCCTT 
TATGCCATCT TGGATCTGTT CACTCCGGAG AACGCCAGCG TGATGACGTC AGAGCAGCAG 
GTGGAGTTGT TTGAGAAGGA CATAGAGTAT CTGAAGACGG AGCTCAACGA CGTCTCGGCC 
ACTGACACAT CCCCTATCAG TCTCTATGAG TACTTCCACA TCTCTCCTAT CAAGCTGCAC 
CTCAGTTTCT CTCTGAGTTC TGGAGGAGAG GATGGTAACA AGGAGAAGAG GGAGACCGAG 
ATCATCCCTG TCCAGTCCCT CAATCTGCTG CTGAAAAGCA TCGGAGCAAC CCTCACTGAC 
GTGCAGGATG TCGTCTTCAA ATTGGCCTTC TTCGAGCTGA CCTTCCAGTT TCGCACTCGC 
CAGCAACTAC AATGGGAGGT CATCAGACAC TACTCGAAAC AGGCGATAAA GCAGATGTAT 
GTGCTGGTGT TGGGCTTGGA CGTGCTGGGA AACCCCTTTG GACTGATCCG AGGTCTGTCG 
GAGGGAGTGG AGGCTTTCTT CTACGAGCCC TATCAGGGAG CTATCCAGGG GCCAGAGGAG 
TTTGTGGAGG GTATGGCGCT GGGGGTGAAA GCGCTGGTTG GAGGAGCAGT AGGTGGCATT 
GCGGGCGCTG CCTCCAGGAT CACAGGCGCC ATGGCGAAGG GTGTGGCTGC CATGACAATG 
GACGAGGAGT ACCAGCAGAA GAGACGGGAG GCTATGAACA AGCAGCCTAG CGGCCTGAGA 
GAGGGCCTGA CCCGCGGAGG CAAAGGACTG GTGTCTGGCT TTGTCAGTGG GATCACAGGG 
ATTGTCACCA AGCCCATTAA AGGTGCTCAG AAGGAGGGCG CGGCGGGCTT CTTTAAGGGT 
GTGGGCAAGG GGCTAGTGGG TGCTGTGGCC AGACCCACTG GAGGCATCAT CGACATGGCC 
AGCAGCACCT TCCAGGGCAT CAAGAGGGCA GCTGAGACAT CCCAGGATGT GGAGAGTCTG 
CGTCCGCCTC GCTTCATCCA TGAGGACGGG GTCATCCGAC CATACAAGGA AAGAGAGGGC 
ATCGGCAGCC AGATGCTACA GAAAATTGAA AACGGAAGGT TCGCCAAGTA CAGATACTTT 
GCGCACGCCA AAGTGAACGA GTCCGATTTC CTGATGATCA CCAAAAGGGG AATCTTTTTC 
GTGACCAAAG GTACATTTGG GCAGCTGACC TGCGAGTGGC AGTATCTGTT CGACGAGTTC 
ACCAAGGAGC CCATGATCGT GGAGGGGAGG CGGCTTCGGA TCGAGGCCAA GGAGAGAGTG 
AAGTCTGTGT TTCATGCCAA AGAGTTTGGG AAGATAATCA ACTTCAGAAG TCCAGAAATA 
GCCAAGTGGG TTCTTACCAA GCTTGAAGAC GCCAGGGAGA GTCTACACAA ATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021476713.1
CDS	274..9990
Translation	MVFESVVVDVLNRFLGDYVVNLDSSQLKLGIWGGDAVLRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDGVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEETKQKAAEQENPALEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPNCPFSFGVSLQNLSLQTADENWMPCLLDEKAKLFFKLVQLDNLFAYWNVNSMLYSNHMPDEALRCLQDSIPGGSTPMNHDFIFRPITADAKLRMNPRSDVDFSSPKVDLVVNLREVAVELNRPQYISILELLGSVDMMSRNLPYRKYRPQVHVHTNAYLWWQYVITGIMEVDVKPRLHMWSWQHIRCHRQTVKQYRELYKAKITSKKPTEKQLRELEEPERTLDIFNITLARQQAEMEASKAGLRIFRPGVKVEEEESQGWLGWMWSGWSGDCGAEAKEVKTGAFDELMTSAEKAKLYTAIGYSEIAVNHNLPKNFEDMKINFHMERMSVSMKDNKDQNEILKLTVEDLKSMLTQRPGAQAIKLSAQLSLFEVTGLASNRPAPTLLSSRKAATVTGTPLLDILFETNPLDESADQRLRVESQPLEIIYDAVTVNSMSAFFSPPDDLQLDDLTNATLMKLEQFRDRTSTGLMYVIETQKVLDLNVNLMASYVIVPQTGFHDSTQNLMLLDLGHFKVSSLSREGLPQLSVGRSTIEDIMSRAYDSFDVQLSSLQFLYSKPDGDWKKARKLRQSALHILEPVDVKVVFSRAMVVTDSRMPKFKISGELPLLSLRISDDKVRGVLELIDSIPLPESRPAPRSTNAHSTSKALKQSYTPQLTPRKPTMAELRNSLIWESGSEEDLFYDAPSSPIGDRPFFPDNMPSPLQRSDSVKRRGLVKKDPKKNMTEFLMTFEISKLSVQLCRLSGGEEVTVLHLDIEGLGTELKLRTFDMTSYTFLREICLECPEYMDSEEKKVHLITTLDNTEDDLLTLEYTKADKNGPEFKSEYKNTEQSVKVNFSSLDIHLHTEALLNAMNFLISLLPPTKEVAPSQEELPTIPEEDEGEGEEGEKKEGSAVAKKTSSKKSKFEDVVNLHIRADLNCLKVFIRGQSHRIAEIRIEGLVSEVLMRKKAVEVLANLKNIVILDCDKEALYKQCVSIADEEVFAFRMVNHTEATEGDAYLDMGKVDTSVTLTVGCIQVIFLNKFVSSILAFINNFQVAKEALAEVTVQAAEKAATGVMELAERSTRISLDVDFNAPVVFLPQSSSSANVIVADLGMVTVKNRFAMVPSQMHVKIPPVMDTMTVGLSDLKMYRTTFEEGRFQGEIQLLKPVNLDLSIQRNLSASWYHSIPDIEITAHLKPMNLILGQDDMTVVLRTLSGNLSETSDSASPPPPPRTVDRSDTASTKGSQASGGTGTNPTSGNTVVTAAVVETQKSTKLKSTLKLDFKFDSMTLVLYSPNGTEVQVLDSRDEQLKLAEFTLGTISTSVHMFSDGSMKASVKLTDCLLDDKRCRVKKITARMMAMRPGAERNVMVEVNYKQGRDGTSLDTVVQDVYLCASMEFLLTVADIFLKASQQGFSSATPKNSSSTKPTAAAASKESAPVGVVAKSEINVLVKNPEIVFVADLTRADAPALVMTTECELVMKSDLDGQLMTAVVKDLKVVACPFLKEKRNKETTVLQPCQVFFKSTQSSTSPQAMEVCINALTLKVSPIIINTVITIQSVLSPAGAETPQSELESPVPPDLWERRSWRDLKLWFLEEEGKDEDAQSLVPLIPQGESLQLAINSICLTLEAGVGHRTVPMLLAKSSFHGDVKNWSTLINLHSQLNLEVHYFNEVMGVWEPLLEPLEDETRDGFRPWTLGLKMKKKPVKYTGISEEVDYSIPDYKTVISISSKDQLNITLSKCGLGMLSNLGTAFAEAAKQASDSFQKDEAPFVVKNRLGLTVSVVHSELFSPLGREATARRVELQDGESLNMDYRRTTTHSDQFSAMTSLVAKDYYIQPTPVGHTSTGMIPLIKVGRGMYSVMHKDSGVTRFLVCQIYSVEGSKYIKIRSPFQIINHFSIPFRVMEGSTSLGISLPTEEFCVPLDSYRSELRLQPVTEGDDQYDCSEGFSYELVSQQPGARVQQTCHRHGDSAGVLMVNIVPLKDTVTSQHTGMAGEHFDEPYILHLWPSVLLRNLLPYPITYRLQDSEEPTPITLMDGHSAQLHTAIMDQSSLELRLLDYLGQDWQAHYGLCGDLDEITFIVFSSLRDEEEVVRLGERGEGKRAELDIAVHVKYDAGQTEVAIHSPYWMVNKTGRLLQYKADDIHRKHAMDYDMPLLFSFKPHNFLQNNKVRLMISDSDLSDDFSLDTVGSYGEVKCKGRLKDYVIGVKIDASSFSLTRIVSFVPYYLLVNRTKHVIWVGEEGQDDWTEARPQQAAIPFWPERDSKKLKVKVEGSRSTPQTMDFTKPENCLLLHLDNTLGGVIMDVNLSDHSIIIRFSDYHDGAAPFLIINHTQEQTVEFYQSSQKEKDKLSPGKAMYYTWAEPTGSRSLAWQCGSYSGELRSEEDLRLDVNEEGKLYLISFYEGLQRVALFTEDRRIYKLLCESEKAELAEQEIILSLQDVGVSLVNNSSRQEVSFIGITSSDVVWESKPKKKSRWKPLSGKDAELLETHFKEYTESGPVDNAILDLENNFQVSLTHNGQDMRMMQPYDAPIRRSFLPGVKVEYSVSARQSAYRVQINRIQIQNQLPGAIFPYVFYPIKLPKSVTMDSEPKPLTDISIVTRAAGHSDISRIKYFKVLIQEMDLKLDLGFLYAILDLFTPENASVMTSEQQVELFEKDIEYLKTELNDVSATDTSPISLYEYFHISPIKLHLSFSLSSGGEDGNKEKRETEIIPVQSLNLLLKSIGATLTDVQDVVFKLAFFELTFQFRTRQQLQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGIAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRAAETSQDVESLRPPRFIHEDGVIRPYKEREGIGSQMLQKIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKGTFGQLTCEWQYLFDEFTKEPMIVEGRRLRIEAKERVKSVFHAKEFGKIINFRSPEIAKWVLTKLEDARESLHKY

Target ORF information:

RefSeq Version	XM_021621038.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621038.1

ORF Insert Sequence:

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGT 
ACTGGTACTA ACCCCACCAG CGGCAACACG GTGGTAACTG CTGCAGTGGT GGAGACCCAG 
AAGAGCACCA AGCTGAAGAG CACACTCAAA CTGGACTTCA AGTTTGACTC CATGACCCTG 
GTCCTGTACA GCCCCAATGG GACTGAGGTG CAGGTGTTGG ACTCGAGGGA TGAGCAGCTG 
AAGCTGGCTG AGTTTACCCT GGGGACTATC TCTACATCTG TCCACATGTT CTCAGACGGA 
TCTATGAAGG CCTCCGTCAA ACTCACAGAC TGCCTGCTAG ACGACAAAAG ATGCAGGGTC 
AAGAAGATCA CTGCCAGGAT GATGGCGATG CGTCCGGGCG CTGAGAGGAA CGTGATGGTG 
GAGGTGAACT ACAAGCAGGG CCGCGACGGC ACCTCTTTGG ACACGGTGGT GCAGGACGTG 
TACCTCTGTG CCAGCATGGA GTTCCTGCTC ACTGTGGCCG ACATCTTCCT CAAAGCCAGC 
CAGCAGGGCT TCTCCTCCGC CACCCCCAAG AACAGCAGTA GCACTAAACC CACTGCAGCC 
GCAGCCTCCA AGGAATCTGC TCCAGTGGGG GTGGTGGCAA AGTCAGAGAT TAATGTTCTG 
GTGAAGAACC CCGAGATTGT GTTTGTGGCC GACCTGACAC GTGCCGACGC CCCGGCCCTG 
GTCATGACCA CGGAGTGTGA GCTGGTGATG AAGAGCGACC TGGACGGTCA GCTGATGACT 
GCGGTGGTCA AGGACCTGAA GGTGGTGGCC TGCCCCTTCC TGAAGGAGAA GAGAAACAAG 
GAGACCACTG TGCTGCAGCC CTGCCAAGTG TTCTTCAAGA GCACACAGTC CTCCACCTCC 
CCGCAGGCCA TGGAGGTCTG CATCAACGCA CTCACACTCA AGGTGTCGCC CATCATTATC 
AACACAGTGA TTACCATTCA GTCGGTGCTG AGCCCGGCCG GTGCCGAGAC ACCCCAGTCT 
GAGCTAGAGA GCCCCGTGCC CCCAGACCTG TGGGAGCGCC GGTCCTGGAG GGACCTCAAG 
CTGTGGTTCC TGGAGGAGGA GGGTAAAGAC GAGGATGCCC AGTCCCTGGT CCCGCTGATT 
CCCCAGGGCG AGTCCCTGCA GTTGGCCATC AACTCCATCT GCCTGACACT GGAGGCAGGG 
GTGGGCCACC GCACCGTCCC CATGCTCCTG GCCAAGTCCT CCTTCCACGG TGACGTCAAG 
AACTGGAGCA CCCTCATCAA CCTGCACAGC CAGCTCAACC TGGAGGTTCA CTACTTCAAC 
GAGGTGATGG GGGTGTGGGA GCCACTGCTA GAGCCCCTAG AAGACGAGAC CCGAGATGGA 
TTCAGACCCT GGACCCTGGG ATTAAAGATG AAGAAGAAGC CAGTGAAGTA CACAGGTATT 
TCAGAAGAAG TGGACTACAG CATCCCTGAC TACAAGACTG TCATCAGCAT CAGCAGCAAG 
GACCAACTTA ATATCACCCT CTCCAAGTGT GGACTGGGCA TGCTCAGTAA CCTGGGCACG 
GCCTTTGCGG AGGCGGCCAA GCAGGCATCG GATAGTTTCC AGAAGGACGA GGCTCCGTTC 
GTCGTGAAGA ACCGTCTAGG TCTGACTGTG TCGGTGGTGC ACAGTGAGCT GTTCAGCCCC 
CTGGGTCGCG AGGCCACGGC GCGCCGGGTA GAGCTGCAGG ACGGAGAGAG CCTCAACATG 
GACTACCGTC GCACCACCAC CCACTCAGAC CAGTTCTCTG CCATGACTTC ATTGGTGGCC 
AAGGACTACT ACATACAGCC CACTCCGGTG GGCCACACTT CAACAGGCAT GATCCCGTTG 
ATCAAAGTGG GGCGTGGCAT GTACAGTGTC ATGCACAAAG ACTCTGGGGT CACCCGTTTC 
CTGGTCTGTC AGATCTACTC TGTGGAGGGA AGCAAGTACA TCAAGATCCG CTCTCCCTTC 
CAGATCATAA ACCACTTCTC CATCCCATTC AGAGTGATGG AGGGCTCTAC CAGTTTGGGG 
ATATCACTGC CTACAGAGGA GTTCTGTGTG CCTCTGGACT CCTACAGGTC AGAGCTAAGA 
CTGCAGCCGG TAACCGAGGG AGACGATCAG TACGACTGCT CCGAGGGTTT CAGCTACGAG 
TTGGTGAGCC AGCAGCCCGG TGCACGTGTG CAGCAGACCT GTCATCGCCA TGGTGACAGT 
GCTGGCGTCC TCATGGTCAA CATCGTCCCT CTGAAAGACA CGGTGACATC ACAACACACA 
GGAATGGCTG GGGAGCACTT TGACGAGCCC TACATCCTGC ACCTGTGGCC CTCCGTCCTG 
CTTCGGAACC TTCTGCCTTA CCCCATCACC TATCGCCTAC AGGACAGTGA AGAGCCCACC 
CCCATCACCC TGATGGACGG CCACTCGGCC CAGCTGCACA CCGCCATCAT GGACCAGTCC 
AGCCTGGAAC TCCGCCTCCT CGACTACCTG GGTCAGGATT GGCAGGCCCA CTACGGTCTC 
TGTGGCGACC TGGATGAGAT CACCTTCATT GTGTTCAGCT CTCTGCGCGA TGAAGAGGAG 
GTGGTCAGGT TAGGAGAGAG GGGAGAAGGG AAGAGGGCAG AGCTCGATAT TGCGGTGCAT 
GTGAAGTACG ACGCGGGCCA GACTGAGGTG GCTATCCATT CTCCCTACTG GATGGTGAAC 
AAGACGGGCC GCCTGCTGCA ATACAAGGCC GATGACATCC ACCGCAAACA CGCCATGGAC 
TACGATATGC CTCTGCTCTT CTCCTTCAAG CCACACAACT TTCTTCAGAA CAACAAGGTG 
CGGCTGATGA TCTCGGACAG CGACCTGTCG GATGATTTCT CCCTGGATAC CGTGGGTAGT 
TATGGTGAAG TGAAGTGCAA AGGGAGGCTG AAAGATTACG TGATCGGAGT GAAGATCGAC 
GCCAGCAGTT TCAGCCTGAC CCGTATCGTA AGCTTTGTGC CATACTACCT GTTAGTGAAC 
AGGACCAAAC ATGTGATCTG GGTGGGAGAG GAGGGCCAAG ACGACTGGAC CGAGGCCAGA 
CCACAACAGG CAGCCATCCC GTTTTGGCCT GAGAGGGACT CCAAGAAGCT CAAAGTCAAA 
GTAGAAGGCA GTCGGTCAAC TCCTCAAACC ATGGACTTCA CCAAACCGGA AAACTGCCTG 
CTGCTGCATC TGGACAACAC TCTGGGCGGG GTCATTATGG ATGTGAACCT ATCGGACCAC 
TCGATTATCA TCCGTTTCTC TGACTACCAC GACGGGGCCG CCCCCTTCCT CATCATCAAC 
CACACCCAGG AGCAGACGGT GGAGTTCTAC CAGAGTAGTC AGAAGGAGAA GGACAAGCTA 
AGCCCCGGGA AGGCAATGTA CTACACCTGG GCTGAGCCCA CTGGATCGCG CTCTCTGGCC 
TGGCAGTGTG GCAGCTACAG CGGCGAGCTG AGGAGCGAAG AGGACCTGCG GTTGGACGTG 
AACGAGGAGG GTAAGCTGTA CCTCATCTCG TTCTACGAGG GCCTCCAGCG GGTGGCGCTG 
TTCACAGAGG ACAGACGCAT ATACAAGCTG CTGTGTGAGA GTGAGAAGGC CGAGCTGGCC 
GAACAAGAGA TCATCCTCTC CCTGCAAGAC GTGGGCGTCT CCCTGGTCAA CAACAGCAGC 
CGCCAGGAGG TGTCCTTCAT CGGTATTACC AGTTCAGACG TGGTGTGGGA GTCCAAGCCA 
AAGAAGAAGT CTCGCTGGAA ACCCCTGAGT GGAAAAGATG CCGAGCTGCT GGAGACCCAC 
TTCAAAGAGT ACACAGAGAG TGGACCAGTG GACAACGCCA TCTTAGACCT GGAGAACAAC 
TTCCAGGTGT CTTTGACCCA TAATGGGCAA GACATGAGGA TGATGCAGCC ATATGACGCT 
CCAATCAGGA GGAGCTTCCT GCCAGGGGTG AAGGTGGAGT ACAGCGTATC GGCCCGCCAG 
AGTGCCTACC GTGTCCAGAT CAACCGCATA CAGATCCAGA ATCAGCTACC TGGAGCAATC 
TTCCCATATG TCTTCTACCC TATAAAACTA CCTAAGTCTG TCACCATGGA CTCAGAGCCC 
AAACCTCTGA CCGACATCAG CATTGTTACC AGGGCAGCAG GGCACTCTGA TATCTCACGC 
ATCAAGTATT TCAAGGTGCT AATCCAGGAG ATGGATCTGA AGTTGGACTT GGGCTTCCTT 
TATGCCATCT TGGATCTGTT CACTCCGGAG AACGCCAGCG TGATGACGTC AGAGCAGCAG 
GTGGAGTTGT TTGAGAAGGA CATAGAGTAT CTGAAGACGG AGCTCAACGA CGTCTCGGCC 
ACTGACACAT CCCCTATCAG TCTCTATGAG TACTTCCACA TCTCTCCTAT CAAGCTGCAC 
CTCAGTTTCT CTCTGAGTTC TGGAGGAGAG GATGGTAACA AGGAGAAGAG GGAGACCGAG 
ATCATCCCTG TCCAGTCCCT CAATCTGCTG CTGAAAAGCA TCGGAGCAAC CCTCACTGAC 
GTGCAGGATG TCGTCTTCAA ATTGGCCTTC TTCGAGCTGA CCTTCCAGTT TCGCACTCGC 
CAGCAACTAC AATGGGAGGT CATCAGACAC TACTCGAAAC AGGCGATAAA GCAGATGTAT 
GTGCTGGTGT TGGGCTTGGA CGTGCTGGGA AACCCCTTTG GACTGATCCG AGGTCTGTCG 
GAGGGAGTGG AGGCTTTCTT CTACGAGCCC TATCAGGGAG CTATCCAGGG GCCAGAGGAG 
TTTGTGGAGG GTATGGCGCT GGGGGTGAAA GCGCTGGTTG GAGGAGCAGT AGGTGGCATT 
GCGGGCGCTG CCTCCAGGAT CACAGGCGCC ATGGCGAAGG GTGTGGCTGC CATGACAATG 
GACGAGGAGT ACCAGCAGAA GAGACGGGAG GCTATGAACA AGCAGCCTAG CGGCCTGAGA 
GAGGGCCTGA CCCGCGGAGG CAAAGGACTG GTGTCTGGCT TTGTCAGTGG GATCACAGGG 
ATTGTCACCA AGCCCATTAA AGGTGCTCAG AAGGAGGGCG CGGCGGGCTT CTTTAAGGGT 
GTGGGCAAGG GGCTAGTGGG TGCTGTGGCC AGACCCACTG GAGGCATCAT CGACATGGCC 
AGCAGCACCT TCCAGGGCAT CAAGAGGGCA GCTGAGACAT CCCAGGATGT GGAGAGTCTG 
CGTCCGCCTC GCTTCATCCA TGAGGACGGG GTCATCCGAC CATACAAGGA AAGAGAGGGC 
ATCGGCAGCC AGATGCTACA GAAAATTGAA AACGGAAGGT TCGCCAAGTA CAGATACTTT 
GCGCACGCCA AAGTGAACGA GTCCGATTTC CTGATGATCA CCAAAAGGGG AATCTTTTTC 
GTGACCAAAG GTACATTTGG GCAGCTGACC TGCGAGTGGC AGTATCTGTT CGACGAGTTC 
ACCAAGGAGC CCATGATCGT GGAGGGGAGG CGGCTTCGGA TCGAGGCCAA GGAGAGAGTG 
AAGTCTGTGT TTCATGCCAA AGAGTTTGGG AAGATAATCA ACTTCAGAAG TCCAGAAATA 
GCCAAGTGGG TTCTTACCAA GCTTGAAGAC GCCAGGGAGA GTCTACACAA ATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps13a ( XM_021621039.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021476714.1 Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X5, mRNA.

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CloneID	OOf56507
Clone ID Related Accession (Same CDS sequence)	XM_021621039.1
Accession Version	XM_021621039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	vacuolar protein sorting-associated protein 13A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGT 
ACTGGTACTA ACCCCACCAG CGGCAACACG GTGGTAACTG CTGCAGTGGT GGAGACCCAG 
AAGAGCACCA AGCTGAAGAG CACACTCAAA CTGGACTTCA AGTTTGACTC CATGACCCTG 
GTCCTGTACA GCCCCAATGG GACTGAGGTG CAGGTGTTGG ACTCGAGGGA TGAGCAGCTG 
AAGCTGGCTG AGTTTACCCT GGGGACTATC TCTACATCTG TCCACATGTT CTCAGACGGA 
TCTATGAAGG CCTCCGTCAA ACTCACAGAC TGCCTGCTAG ACGACAAAAG ATGCAGGGTC 
AAGAAGATCA CTGCCAGGAT GATGGCGATG CGTCCGGGCG CTGAGAGGAA CGTGATGGTG 
GAGGTGAACT ACAAGCAGGG CCGCGACGGC ACCTCTTTGG ACACGGTGGT GCAGGACGTG 
TACCTCTGTG CCAGCATGGA GTTCCTGCTC ACTGTGGCCG ACATCTTCCT CAAAGCCAGC 
CAGCAGGGCT TCTCCTCCGC CACCCCCAAG AACAGCAGTA GCACTAAACC CACTGCAGCC 
GCAGCCTCCA AGGAATCTGC TCCAGTGGGG GTGGTGGCAA AGTCAGAGAT TAATGTTCTG 
GTGAAGAACC CCGAGATTGT GTTTGTGGCC GACCTGACAC GTGCCGACGC CCCGGCCCTG 
GTCATGACCA CGGAGTGTGA GCTGGTGATG AAGAGCGACC TGGACGGTCA GCTGATGACT 
GCGGTGGTCA AGGACCTGAA GGTGGTGGCC TGCCCCTTCC TGAAGGAGAA GAGAAACAAG 
GAGACCACTG TGCTGCAGCC CTGCCAAGTG TTCTTCAAGA GCACACAGTC CTCCACCTCC 
CCGCAGGCCA TGGAGGTCTG CATCAACGCA CTCACACTCA AGGTGTCGCC CATCATTATC 
AACACAGTGA TTACCATTCA GTCGGTGCTG AGCCCGGCCG GTGCCGAGAC ACCCCAGTCT 
GAGCTAGAGA GCCCCGTGCC CCCAGACCTG TGGGAGCGCC GGTCCTGGAG GGACCTCAAG 
CTGTGGTTCC TGGAGGAGGA GGGTAAAGAC GAGGATGCCC AGTCCCTGGT CCCGCTGATT 
CCCCAGGGCG AGTCCCTGCA GTTGGCCATC AACTCCATCT GCCTGACACT GGAGGCAGGG 
GTGGGCCACC GCACCGTCCC CATGCTCCTG GCCAAGTCCT CCTTCCACGG TGACGTCAAG 
AACTGGAGCA CCCTCATCAA CCTGCACAGC CAGCTCAACC TGGAGGTTCA CTACTTCAAC 
GAGGTGATGG GGGTGTGGGA GCCACTGCTA GAGCCCCTAG AAGACGAGAC CCGAGATGGA 
TTCAGACCCT GGACCCTGGG ATTAAAGATG AAGAAGAAGC CAGTGAAGTA CACAGGTATT 
TCAGAAGAAG TGGACTACAG CATCCCTGAC TACAAGACTG TCATCAGCAT CAGCAGCAAG 
GACCAACTTA ATATCACCCT CTCCAAGTGT GGACTGGGCA TGCTCAGTAA CCTGGGCACG 
GCCTTTGCGG AGGCGGCCAA GCAGGCATCG GATAGTTTCC AGAAGGACGA GGCTCCGTTC 
GTCGTGAAGA ACCGTCTAGG TCTGACTGTG TCGGTGGTGC ACAGTGAGCT GTTCAGCCCC 
CTGGGTCGCG AGGCCACGGC GCGCCGGGTA GAGCTGCAGG ACGGAGAGAG CCTCAACATG 
GACTACCGTC GCACCACCAC CCACTCAGAC CAGTTCTCTG CCATGACTTC ATTGGTGGCC 
AAGGACTACT ACATACAGCC CACTCCGGTG GGCCACACTT CAACAGGCAT GATCCCGTTG 
ATCAAAGTGG GGCGTGGCAT GTACAGTGTC ATGCACAAAG ACTCTGGGGT CACCCGTTTC 
CTGGTCTGTC AGATCTACTC TGTGGAGGGA AGCAAGTACA TCAAGATCCG CTCTCCCTTC 
CAGATCATAA ACCACTTCTC CATCCCATTC AGAGTGATGG AGGGCTCTAC CAGTTTGGGG 
ATATCACTGC CTACAGAGGA GTTCTGTGTG CCTCTGGACT CCTACAGGTC AGAGCTAAGA 
CTGCAGCCGG TAACCGAGGG AGACGATCAG TACGACTGCT CCGAGGGTTT CAGCTACGAG 
TTGGTGAGCC AGCAGCCCGG TGCACGTGTG CAGCAGACCT GTCATCGCCA TGGTGACAGT 
GCTGGCGTCC TCATGGTCAA CATCGTCCCT CTGAAAGACA CGGTGACATC ACAACACACA 
GGAATGGCTG GGGAGCACTT TGACGAGCCC TACATCCTGC ACCTGTGGCC CTCCGTCCTG 
CTTCGGAACC TTCTGCCTTA CCCCATCACC TATCGCCTAC AGGACAGTGA AGAGCCCACC 
CCCATCACCC TGATGGACGG CCACTCGGCC CAGCTGCACA CCGCCATCAT GGACCAGTCC 
AGCCTGGAAC TCCGCCTCCT CGACTACCTG GGTCAGGATT GGCAGGCCCA CTACGGTCTC 
TGTGGCGACC TGGATGAGAT CACCTTCATT GTGTTCAGCT CTCTGCGCGA TGAAGAGGAG 
GTGGTCAGGT TAGGAGAGAG GGGAGAAGGG AAGAGGGCAG AGCTCGATAT TGCGGTGCAT 
GTGAAGTACG ACGCGGGCCA GACTGAGGTG GCTATCCATT CTCCCTACTG GATGGTGAAC 
AAGACGGGCC GCCTGCTGCA ATACAAGGCC GATGACATCC ACCGCAAACA CGCCATGGAC 
TACGATATGC CTCTGCTCTT CTCCTTCAAG CCACACAACT TTCTTCAGAA CAACAAGGTG 
CGGCTGATGA TCTCGGACAG CGACCTGTCG GATGATTTCT CCCTGGATAC CGTGGGTAGT 
TATGGTGAAG TGAAGTGCAA AGGGAGGCTG AAAGATTACG TGATCGGAGT GAAGATCGAC 
GCCAGCAGTT TCAGCCTGAC CCGTATCGTA AGCTTTGTGC CATACTACCT GTTAGTGAAC 
AGGACCAAAC ATGTGATCTG GGTGGGAGAG GAGGGCCAAG ACGACTGGAC CGAGGCCAGA 
CCACAACAGG CAGCCATCCC GTTTTGGCCT GAGAGGGACT CCAAGAAGCT CAAAGTCAAA 
GTAGAAGGCA GTCGGTCAAC TCCTCAAACC ATGGACTTCA CCAAACCGGA AAACTGCCTG 
CTGCTGCATC TGGACAACAC TCTGGGCGGG GTCATTATGG ATGTGAACCT ATCGGACCAC 
TCGATTATCA TCCGTTTCTC TGACTACCAC GACGGGGCCG CCCCCTTCCT CATCATCAAC 
CACACCCAGG AGCAGACGGT GGAGTTCTAC CAGAGATTTC GGAGAGATTC ATCAGAATGG 
GTGGCAGACG AGCTGCTCGG CCTCTCCTTC TCAGTAGAGG AGACCCAGCC CAGTAGTCAG 
AAGGAGAAGG ACAAGCTAAG CCCCGGGAAG GCAATGTACT ACACCTGGGC TGAGCCCACT 
GGATCGCGCT CTCTGGCCTG GCAGTGTGGC AGCTACAGCG GCGAGCTGAG GAGCGAAGAG 
GACCTGCGGT TGGACGTGAA CGAGGAGGGT AAGCTGTACC TCATCTCGTT CTACGAGGGC 
CTCCAGCGGG TGGCGCTGTT CACAGAGGAC AGACGCATAT ACAAGCTGCT GTGTGAGAGT 
GAGAAGGCCG AGCTGGCCGA ACAAGAGATC ATCCTCTCCC TGCAAGACGT GGGCGTCTCC 
CTGGTCAACA ACAGCAGCCG CCAGGAGGTG TCCTTCATCG GTATTACCAG TTCAGACGTG 
GTGTGGGAGT CCAAGCCAAA GAAGAAGTCT CGCTGGAAAC CCCTGAGTGG AAAAGATGCC 
GAGCTGCTGG AGACCCACTT CAAAGAGTAC ACAGAGAGTG GACCAGTGGA CAACGCCATC 
TTAGACCTGG AGAACAACTT CCAGGTGTCT TTGACCCATA ATGGGCAAGA CATGAGGATG 
ATGCAGCCAT ATGACGCTCC AATCAGGAGG AGCTTCCTGC CAGGGGTGAA GGTGGAGTAC 
AGCGTATCGG CCCGCCAGAG TGCCTACCGT GTCCAGATCA ACCGCATACA GATCCAGAAT 
CAGCTACCTG GAGCAATCTT CCCATATGTC TTCTACCCTA TAAAACTACC TAAGTCTGTC 
ACCATGGACT CAGAGCCCAA ACCTCTGACC GACATCAGCA TTGTTACCAG GGCAGCAGGG 
CACTCTGATA TCTCACGCAT CAAGTATTTC AAGGTGCTAA TCCAGGAGAT GGATCTGAAG 
TTGGACTTGG GCTTCCTTTA TGCCATCTTG GATCTGTTCA CTCCGGAGAA CGCCAGCGTG 
ATGACGTCAG AGCAGCAGGT GGAGTTGTTT GAGAAGGACA TAGAGTATCT GAAGACGGAG 
CTCAACGACG TCTCGGCCAC TGACACATCC CCTATCAGTC TCTATGAGTA CTTCCACATC 
TCTCCTATCA AGCTGCACCT CAGTTTCTCT CTGAGTTCTG GAGGAGAGGA TGGTAACAAG 
GAGAAGAGGG AGACCGAGAT CATCCCTGTC CAGTCCCTCA ATCTGCTGCT GAAAAGCATC 
GGAGCAACCC TCACTGACGT GCAGGATGTC GTCTTCAAAT TGGCCTTCTT CGAGCTGACC 
TTCCAGTTTC GCACTCGCCA GCAACTACAA TGGGAGGTCA TCAGACACTA CTCGAAACAG 
GCGATAAAGC AGATGTATGT GCTGGTGTTG GGCTTGGACG TGCTGGGAAA CCCCTTTGGA 
CTGATCCGAG GTCTGTCGGA GGGAGTGGAG GCTTTCTTCT ACGAGCCCTA TCAGGGAGCT 
ATCCAGGGGC CAGAGGAGTT TGTGGAGGGT ATGGCGCTGG GGGTGAAAGC GCTGGTTGGA 
GGAGCAGTAG GTGGCATTGC GGGCGCTGCC TCCAGGATCA CAGGCGCCAT GGCGAAGGGT 
GTGGCTGCCA TGACAATGGA CGAGGAGTAC CAGCAGAAGA GACGGGAGGC TATGAACAAG 
CAGCCTAGCG GCCTGAGAGA GGGCCTGACC CGCGGAGGCA AAGGACTGGT GTCTGGCTTT 
GTCAGTGGGA TCACAGGGAT TGTCACCAAG CCCATTAAAG GTGCTCAGAA GGAGGGCGCG 
GCGGGCTTCT TTAAGGGTGT GGGCAAGGGG CTAGTGGGTG CTGTGGCCAG ACCCACTGGA 
GGCATCATCG ACATGGCCAG CAGCACCTTC CAGGGCATCA AGAGGGCAGC TGAGACATCC 
CAGGATGTGG AGAGTCTGCG TCCGCCTCGC TTCATCCATG AGGACGGGGT CATCCGACCA 
TACAAGGAAA GAGAGGGCAT CGGCAGCCAG ATGCTACAGA AAACGGTGGC CTTCACTGAA 
GGGAGTAGAA CTGTCAGTGA TGATGATGAT GATGATGAAC AAGCGCTATA CATATCAAAT 
GTGTTATTGT TACTATTGTT GATGTGGATG ATAACAAGTA GAATGGAATT GATAAGCACT 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021476714.1
CDS	274..9879
Translation	MVFESVVVDVLNRFLGDYVVNLDSSQLKLGIWGGDAVLRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDGVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEETKQKAAEQENPALEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPNCPFSFGVSLQNLSLQTADENWMPCLLDEKAKLFFKLVQLDNLFAYWNVNSMLYSNHMPDEALRCLQDSIPGGSTPMNHDFIFRPITADAKLRMNPRSDVDFSSPKVDLVVNLREVAVELNRPQYISILELLGSVDMMSRNLPYRKYRPQVHVHTNAYLWWQYVITGIMEVDVKPRLHMWSWQHIRCHRQTVKQYRELYKAKITSKKPTEKQLRELEEPERTLDIFNITLARQQAEMEASKAGLRIFRPGVKVEEEESQGWLGWMWSGWSGDCGAEAKEVKTGAFDELMTSAEKAKLYTAIGYSEIAVNHNLPKNFEDMKINFHMERMSVSMKDNKDQNEILKLTVEDLKSMLTQRPGAQAIKLSAQLSLFEVTGLASNRPAPTLLSSRKAATVTGTPLLDILFETNPLDESADQRLRVESQPLEIIYDAVTVNSMSAFFSPPDDLQLDDLTNATLMKLEQFRDRTSTGLMYVIETQKVLDLNVNLMASYVIVPQTGFHDSTQNLMLLDLGHFKVSSLSREGLPQLSVGRSTIEDIMSRAYDSFDVQLSSLQFLYSKPDGDWKKARKLRQSALHILEPVDVKVVFSRAMVVTDSRMPKFKISGELPLLSLRISDDKVRGVLELIDSIPLPESRPAPRSTNAHSTSKALKQSYTPQLTPRKPTMAELRNSLIWESGSEEDLFYDAPSSPIGDRPFFPDNMPSPLQRSDSVKRRGLVKKDPKKNMTEFLMTFEISKLSVQLCRLSGGEEVTVLHLDIEGLGTELKLRTFDMTSYTFLREICLECPEYMDSEEKKVHLITTLDNTEDDLLTLEYTKADKNGPEFKSEYKNTEQSVKVNFSSLDIHLHTEALLNAMNFLISLLPPTKEVAPSQEELPTIPEEDEGEGEEGEKKEGSAVAKKTSSKKSKFEDVVNLHIRADLNCLKVFIRGQSHRIAEIRIEGLVSEVLMRKKAVEVLANLKNIVILDCDKEALYKQCVSIADEEVFAFRMVNHTEATEGDAYLDMGKVDTSVTLTVGCIQVIFLNKFVSSILAFINNFQVAKEALAEVTVQAAEKAATGVMELAERSTRISLDVDFNAPVVFLPQSSSSANVIVADLGMVTVKNRFAMVPSQMHVKIPPVMDTMTVGLSDLKMYRTTFEEGRFQGEIQLLKPVNLDLSIQRNLSASWYHSIPDIEITAHLKPMNLILGQDDMTVVLRTLSGNLSETSDSASPPPPPRTVDRSDTASTKGSQASGGTGTNPTSGNTVVTAAVVETQKSTKLKSTLKLDFKFDSMTLVLYSPNGTEVQVLDSRDEQLKLAEFTLGTISTSVHMFSDGSMKASVKLTDCLLDDKRCRVKKITARMMAMRPGAERNVMVEVNYKQGRDGTSLDTVVQDVYLCASMEFLLTVADIFLKASQQGFSSATPKNSSSTKPTAAAASKESAPVGVVAKSEINVLVKNPEIVFVADLTRADAPALVMTTECELVMKSDLDGQLMTAVVKDLKVVACPFLKEKRNKETTVLQPCQVFFKSTQSSTSPQAMEVCINALTLKVSPIIINTVITIQSVLSPAGAETPQSELESPVPPDLWERRSWRDLKLWFLEEEGKDEDAQSLVPLIPQGESLQLAINSICLTLEAGVGHRTVPMLLAKSSFHGDVKNWSTLINLHSQLNLEVHYFNEVMGVWEPLLEPLEDETRDGFRPWTLGLKMKKKPVKYTGISEEVDYSIPDYKTVISISSKDQLNITLSKCGLGMLSNLGTAFAEAAKQASDSFQKDEAPFVVKNRLGLTVSVVHSELFSPLGREATARRVELQDGESLNMDYRRTTTHSDQFSAMTSLVAKDYYIQPTPVGHTSTGMIPLIKVGRGMYSVMHKDSGVTRFLVCQIYSVEGSKYIKIRSPFQIINHFSIPFRVMEGSTSLGISLPTEEFCVPLDSYRSELRLQPVTEGDDQYDCSEGFSYELVSQQPGARVQQTCHRHGDSAGVLMVNIVPLKDTVTSQHTGMAGEHFDEPYILHLWPSVLLRNLLPYPITYRLQDSEEPTPITLMDGHSAQLHTAIMDQSSLELRLLDYLGQDWQAHYGLCGDLDEITFIVFSSLRDEEEVVRLGERGEGKRAELDIAVHVKYDAGQTEVAIHSPYWMVNKTGRLLQYKADDIHRKHAMDYDMPLLFSFKPHNFLQNNKVRLMISDSDLSDDFSLDTVGSYGEVKCKGRLKDYVIGVKIDASSFSLTRIVSFVPYYLLVNRTKHVIWVGEEGQDDWTEARPQQAAIPFWPERDSKKLKVKVEGSRSTPQTMDFTKPENCLLLHLDNTLGGVIMDVNLSDHSIIIRFSDYHDGAAPFLIINHTQEQTVEFYQRFRRDSSEWVADELLGLSFSVEETQPSSQKEKDKLSPGKAMYYTWAEPTGSRSLAWQCGSYSGELRSEEDLRLDVNEEGKLYLISFYEGLQRVALFTEDRRIYKLLCESEKAELAEQEIILSLQDVGVSLVNNSSRQEVSFIGITSSDVVWESKPKKKSRWKPLSGKDAELLETHFKEYTESGPVDNAILDLENNFQVSLTHNGQDMRMMQPYDAPIRRSFLPGVKVEYSVSARQSAYRVQINRIQIQNQLPGAIFPYVFYPIKLPKSVTMDSEPKPLTDISIVTRAAGHSDISRIKYFKVLIQEMDLKLDLGFLYAILDLFTPENASVMTSEQQVELFEKDIEYLKTELNDVSATDTSPISLYEYFHISPIKLHLSFSLSSGGEDGNKEKRETEIIPVQSLNLLLKSIGATLTDVQDVVFKLAFFELTFQFRTRQQLQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGIAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRAAETSQDVESLRPPRFIHEDGVIRPYKEREGIGSQMLQKTVAFTEGSRTVSDDDDDDEQALYISNVLLLLLLMWMITSRMELISTS

Target ORF information:

RefSeq Version	XM_021621039.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621039.1

ORF Insert Sequence:

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGT 
ACTGGTACTA ACCCCACCAG CGGCAACACG GTGGTAACTG CTGCAGTGGT GGAGACCCAG 
AAGAGCACCA AGCTGAAGAG CACACTCAAA CTGGACTTCA AGTTTGACTC CATGACCCTG 
GTCCTGTACA GCCCCAATGG GACTGAGGTG CAGGTGTTGG ACTCGAGGGA TGAGCAGCTG 
AAGCTGGCTG AGTTTACCCT GGGGACTATC TCTACATCTG TCCACATGTT CTCAGACGGA 
TCTATGAAGG CCTCCGTCAA ACTCACAGAC TGCCTGCTAG ACGACAAAAG ATGCAGGGTC 
AAGAAGATCA CTGCCAGGAT GATGGCGATG CGTCCGGGCG CTGAGAGGAA CGTGATGGTG 
GAGGTGAACT ACAAGCAGGG CCGCGACGGC ACCTCTTTGG ACACGGTGGT GCAGGACGTG 
TACCTCTGTG CCAGCATGGA GTTCCTGCTC ACTGTGGCCG ACATCTTCCT CAAAGCCAGC 
CAGCAGGGCT TCTCCTCCGC CACCCCCAAG AACAGCAGTA GCACTAAACC CACTGCAGCC 
GCAGCCTCCA AGGAATCTGC TCCAGTGGGG GTGGTGGCAA AGTCAGAGAT TAATGTTCTG 
GTGAAGAACC CCGAGATTGT GTTTGTGGCC GACCTGACAC GTGCCGACGC CCCGGCCCTG 
GTCATGACCA CGGAGTGTGA GCTGGTGATG AAGAGCGACC TGGACGGTCA GCTGATGACT 
GCGGTGGTCA AGGACCTGAA GGTGGTGGCC TGCCCCTTCC TGAAGGAGAA GAGAAACAAG 
GAGACCACTG TGCTGCAGCC CTGCCAAGTG TTCTTCAAGA GCACACAGTC CTCCACCTCC 
CCGCAGGCCA TGGAGGTCTG CATCAACGCA CTCACACTCA AGGTGTCGCC CATCATTATC 
AACACAGTGA TTACCATTCA GTCGGTGCTG AGCCCGGCCG GTGCCGAGAC ACCCCAGTCT 
GAGCTAGAGA GCCCCGTGCC CCCAGACCTG TGGGAGCGCC GGTCCTGGAG GGACCTCAAG 
CTGTGGTTCC TGGAGGAGGA GGGTAAAGAC GAGGATGCCC AGTCCCTGGT CCCGCTGATT 
CCCCAGGGCG AGTCCCTGCA GTTGGCCATC AACTCCATCT GCCTGACACT GGAGGCAGGG 
GTGGGCCACC GCACCGTCCC CATGCTCCTG GCCAAGTCCT CCTTCCACGG TGACGTCAAG 
AACTGGAGCA CCCTCATCAA CCTGCACAGC CAGCTCAACC TGGAGGTTCA CTACTTCAAC 
GAGGTGATGG GGGTGTGGGA GCCACTGCTA GAGCCCCTAG AAGACGAGAC CCGAGATGGA 
TTCAGACCCT GGACCCTGGG ATTAAAGATG AAGAAGAAGC CAGTGAAGTA CACAGGTATT 
TCAGAAGAAG TGGACTACAG CATCCCTGAC TACAAGACTG TCATCAGCAT CAGCAGCAAG 
GACCAACTTA ATATCACCCT CTCCAAGTGT GGACTGGGCA TGCTCAGTAA CCTGGGCACG 
GCCTTTGCGG AGGCGGCCAA GCAGGCATCG GATAGTTTCC AGAAGGACGA GGCTCCGTTC 
GTCGTGAAGA ACCGTCTAGG TCTGACTGTG TCGGTGGTGC ACAGTGAGCT GTTCAGCCCC 
CTGGGTCGCG AGGCCACGGC GCGCCGGGTA GAGCTGCAGG ACGGAGAGAG CCTCAACATG 
GACTACCGTC GCACCACCAC CCACTCAGAC CAGTTCTCTG CCATGACTTC ATTGGTGGCC 
AAGGACTACT ACATACAGCC CACTCCGGTG GGCCACACTT CAACAGGCAT GATCCCGTTG 
ATCAAAGTGG GGCGTGGCAT GTACAGTGTC ATGCACAAAG ACTCTGGGGT CACCCGTTTC 
CTGGTCTGTC AGATCTACTC TGTGGAGGGA AGCAAGTACA TCAAGATCCG CTCTCCCTTC 
CAGATCATAA ACCACTTCTC CATCCCATTC AGAGTGATGG AGGGCTCTAC CAGTTTGGGG 
ATATCACTGC CTACAGAGGA GTTCTGTGTG CCTCTGGACT CCTACAGGTC AGAGCTAAGA 
CTGCAGCCGG TAACCGAGGG AGACGATCAG TACGACTGCT CCGAGGGTTT CAGCTACGAG 
TTGGTGAGCC AGCAGCCCGG TGCACGTGTG CAGCAGACCT GTCATCGCCA TGGTGACAGT 
GCTGGCGTCC TCATGGTCAA CATCGTCCCT CTGAAAGACA CGGTGACATC ACAACACACA 
GGAATGGCTG GGGAGCACTT TGACGAGCCC TACATCCTGC ACCTGTGGCC CTCCGTCCTG 
CTTCGGAACC TTCTGCCTTA CCCCATCACC TATCGCCTAC AGGACAGTGA AGAGCCCACC 
CCCATCACCC TGATGGACGG CCACTCGGCC CAGCTGCACA CCGCCATCAT GGACCAGTCC 
AGCCTGGAAC TCCGCCTCCT CGACTACCTG GGTCAGGATT GGCAGGCCCA CTACGGTCTC 
TGTGGCGACC TGGATGAGAT CACCTTCATT GTGTTCAGCT CTCTGCGCGA TGAAGAGGAG 
GTGGTCAGGT TAGGAGAGAG GGGAGAAGGG AAGAGGGCAG AGCTCGATAT TGCGGTGCAT 
GTGAAGTACG ACGCGGGCCA GACTGAGGTG GCTATCCATT CTCCCTACTG GATGGTGAAC 
AAGACGGGCC GCCTGCTGCA ATACAAGGCC GATGACATCC ACCGCAAACA CGCCATGGAC 
TACGATATGC CTCTGCTCTT CTCCTTCAAG CCACACAACT TTCTTCAGAA CAACAAGGTG 
CGGCTGATGA TCTCGGACAG CGACCTGTCG GATGATTTCT CCCTGGATAC CGTGGGTAGT 
TATGGTGAAG TGAAGTGCAA AGGGAGGCTG AAAGATTACG TGATCGGAGT GAAGATCGAC 
GCCAGCAGTT TCAGCCTGAC CCGTATCGTA AGCTTTGTGC CATACTACCT GTTAGTGAAC 
AGGACCAAAC ATGTGATCTG GGTGGGAGAG GAGGGCCAAG ACGACTGGAC CGAGGCCAGA 
CCACAACAGG CAGCCATCCC GTTTTGGCCT GAGAGGGACT CCAAGAAGCT CAAAGTCAAA 
GTAGAAGGCA GTCGGTCAAC TCCTCAAACC ATGGACTTCA CCAAACCGGA AAACTGCCTG 
CTGCTGCATC TGGACAACAC TCTGGGCGGG GTCATTATGG ATGTGAACCT ATCGGACCAC 
TCGATTATCA TCCGTTTCTC TGACTACCAC GACGGGGCCG CCCCCTTCCT CATCATCAAC 
CACACCCAGG AGCAGACGGT GGAGTTCTAC CAGAGATTTC GGAGAGATTC ATCAGAATGG 
GTGGCAGACG AGCTGCTCGG CCTCTCCTTC TCAGTAGAGG AGACCCAGCC CAGTAGTCAG 
AAGGAGAAGG ACAAGCTAAG CCCCGGGAAG GCAATGTACT ACACCTGGGC TGAGCCCACT 
GGATCGCGCT CTCTGGCCTG GCAGTGTGGC AGCTACAGCG GCGAGCTGAG GAGCGAAGAG 
GACCTGCGGT TGGACGTGAA CGAGGAGGGT AAGCTGTACC TCATCTCGTT CTACGAGGGC 
CTCCAGCGGG TGGCGCTGTT CACAGAGGAC AGACGCATAT ACAAGCTGCT GTGTGAGAGT 
GAGAAGGCCG AGCTGGCCGA ACAAGAGATC ATCCTCTCCC TGCAAGACGT GGGCGTCTCC 
CTGGTCAACA ACAGCAGCCG CCAGGAGGTG TCCTTCATCG GTATTACCAG TTCAGACGTG 
GTGTGGGAGT CCAAGCCAAA GAAGAAGTCT CGCTGGAAAC CCCTGAGTGG AAAAGATGCC 
GAGCTGCTGG AGACCCACTT CAAAGAGTAC ACAGAGAGTG GACCAGTGGA CAACGCCATC 
TTAGACCTGG AGAACAACTT CCAGGTGTCT TTGACCCATA ATGGGCAAGA CATGAGGATG 
ATGCAGCCAT ATGACGCTCC AATCAGGAGG AGCTTCCTGC CAGGGGTGAA GGTGGAGTAC 
AGCGTATCGG CCCGCCAGAG TGCCTACCGT GTCCAGATCA ACCGCATACA GATCCAGAAT 
CAGCTACCTG GAGCAATCTT CCCATATGTC TTCTACCCTA TAAAACTACC TAAGTCTGTC 
ACCATGGACT CAGAGCCCAA ACCTCTGACC GACATCAGCA TTGTTACCAG GGCAGCAGGG 
CACTCTGATA TCTCACGCAT CAAGTATTTC AAGGTGCTAA TCCAGGAGAT GGATCTGAAG 
TTGGACTTGG GCTTCCTTTA TGCCATCTTG GATCTGTTCA CTCCGGAGAA CGCCAGCGTG 
ATGACGTCAG AGCAGCAGGT GGAGTTGTTT GAGAAGGACA TAGAGTATCT GAAGACGGAG 
CTCAACGACG TCTCGGCCAC TGACACATCC CCTATCAGTC TCTATGAGTA CTTCCACATC 
TCTCCTATCA AGCTGCACCT CAGTTTCTCT CTGAGTTCTG GAGGAGAGGA TGGTAACAAG 
GAGAAGAGGG AGACCGAGAT CATCCCTGTC CAGTCCCTCA ATCTGCTGCT GAAAAGCATC 
GGAGCAACCC TCACTGACGT GCAGGATGTC GTCTTCAAAT TGGCCTTCTT CGAGCTGACC 
TTCCAGTTTC GCACTCGCCA GCAACTACAA TGGGAGGTCA TCAGACACTA CTCGAAACAG 
GCGATAAAGC AGATGTATGT GCTGGTGTTG GGCTTGGACG TGCTGGGAAA CCCCTTTGGA 
CTGATCCGAG GTCTGTCGGA GGGAGTGGAG GCTTTCTTCT ACGAGCCCTA TCAGGGAGCT 
ATCCAGGGGC CAGAGGAGTT TGTGGAGGGT ATGGCGCTGG GGGTGAAAGC GCTGGTTGGA 
GGAGCAGTAG GTGGCATTGC GGGCGCTGCC TCCAGGATCA CAGGCGCCAT GGCGAAGGGT 
GTGGCTGCCA TGACAATGGA CGAGGAGTAC CAGCAGAAGA GACGGGAGGC TATGAACAAG 
CAGCCTAGCG GCCTGAGAGA GGGCCTGACC CGCGGAGGCA AAGGACTGGT GTCTGGCTTT 
GTCAGTGGGA TCACAGGGAT TGTCACCAAG CCCATTAAAG GTGCTCAGAA GGAGGGCGCG 
GCGGGCTTCT TTAAGGGTGT GGGCAAGGGG CTAGTGGGTG CTGTGGCCAG ACCCACTGGA 
GGCATCATCG ACATGGCCAG CAGCACCTTC CAGGGCATCA AGAGGGCAGC TGAGACATCC 
CAGGATGTGG AGAGTCTGCG TCCGCCTCGC TTCATCCATG AGGACGGGGT CATCCGACCA 
TACAAGGAAA GAGAGGGCAT CGGCAGCCAG ATGCTACAGA AAACGGTGGC CTTCACTGAA 
GGGAGTAGAA CTGTCAGTGA TGATGATGAT GATGATGAAC AAGCGCTATA CATATCAAAT 
GTGTTATTGT TACTATTGTT GATGTGGATG ATAACAAGTA GAATGGAATT GATAAGCACT 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps13a ( XM_021621040.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021476715.1 Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X6, mRNA.

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CloneID	OOf56508
Clone ID Related Accession (Same CDS sequence)	XM_021621040.1
Accession Version	XM_021621040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	vacuolar protein sorting-associated protein 13A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGT 
ACTGGTACTA ACCCCACCAG CGGCAACACG GTGGTAACTG CTGCAGTGGT GGAGACCCAG 
AAGAGCACCA AGCTGAAGAG CACACTCAAA CTGGACTTCA AGTTTGACTC CATGACCCTG 
GTCCTGTACA GCCCCAATGG GACTGAGGTG CAGGTGTTGG ACTCGAGGGA TGAGCAGCTG 
AAGCTGGCTG AGTTTACCCT GGGGACTATC TCTACATCTG TCCACATGTT CTCAGACGGA 
TCTATGAAGG CCTCCGTCAA ACTCACAGAC TGCCTGCTAG ACGACAAAAG ATGCAGGGTC 
AAGAAGATCA CTGCCAGGAT GATGGCGATG CGTCCGGGCG CTGAGAGGAA CGTGATGGTG 
GAGGTGAACT ACAAGCAGGG CCGCGACGGC ACCTCTTTGG ACACGGTGGT GCAGGACGTG 
TACCTCTGTG CCAGCATGGA GTTCCTGCTC ACTGTGGCCG ACATCTTCCT CAAAGCCAGC 
CAGCAGGGCT TCTCCTCCGC CACCCCCAAG AACAGCAGTA GCACTAAACC CACTGCAGCC 
GCAGCCTCCA AGGAATCTGC TCCAGTGGGG GTGGTGGCAA AGTCAGAGAT TAATGTTCTG 
GTGAAGAACC CCGAGATTGT GTTTGTGGCC GACCTGACAC GTGCCGACGC CCCGGCCCTG 
GTCATGACCA CGGAGTGTGA GCTGGTGATG AAGAGCGACC TGGACGGTCA GCTGATGACT 
GCGGTGGTCA AGGACCTGAA GGTGGTGGCC TGCCCCTTCC TGAAGGAGAA GAGAAACAAG 
GAGACCACTG TGCTGCAGCC CTGCCAAGTG TTCTTCAAGA GCACACAGTC CTCCACCTCC 
CCGCAGGCCA TGGAGGTCTG CATCAACGCA CTCACACTCA AGGTGTCGCC CATCATTATC 
AACACAGTGA TTACCATTCA GTCGGTGCTG AGCCCGGCCG GTGCCGAGAC ACCCCAGTCT 
GAGCTAGAGA GCCCCGTGCC CCCAGACCTG TGGGAGCGCC GGTCCTGGAG GGACCTCAAG 
CTGTGGTTCC TGGAGGAGGA GGGTAAAGAC GAGGATGCCC AGTCCCTGGT CCCGCTGATT 
CCCCAGGGCG AGTCCCTGCA GTTGGCCATC AACTCCATCT GCCTGACACT GGAGGCAGGG 
GTGGGCCACC GCACCGTCCC CATGCTCCTG GCCAAGTCCT CCTTCCACGG TGACGTCAAG 
AACTGGAGCA CCCTCATCAA CCTGCACAGC CAGCTCAACC TGGAGGTTCA CTACTTCAAC 
GAGGTGATGG GGGTGTGGGA GCCACTGCTA GAGCCCCTAG AAGACGAGAC CCGAGATGGA 
TTCAGACCCT GGACCCTGGG ATTAAAGATG AAGAAGAAGC CAGTGAAGTA CACAGGTATT 
TCAGAAGAAG TGGACTACAG CATCCCTGAC TACAAGACTG TCATCAGCAT CAGCAGCAAG 
GACCAACTTA ATATCACCCT CTCCAAGTGT GGACTGGGCA TGCTCAGTAA CCTGGGCACG 
GCCTTTGCGG AGGCGGCCAA GCAGGCATCG GATAGTTTCC AGAAGGACGA GGCTCCGTTC 
GTCGTGAAGA ACCGTCTAGG TCTGACTGTG TCGGTGGTGC ACAGTGAGCT GTTCAGCCCC 
CTGGGTCGCG AGGCCACGGC GCGCCGGGTA GAGCTGCAGG ACGGAGAGAG CCTCAACATG 
GACTACCGTC GCACCACCAC CCACTCAGAC CAGTTCTCTG CCATGACTTC ATTGGTGGCC 
AAGGACTACT ACATACAGCC CACTCCGGTG GGCCACACTT CAACAGGCAT GATCCCGTTG 
ATCAAAGTGG GGCGTGGCAT GTACAGTGTC ATGCACAAAG ACTCTGGGGT CACCCGTTTC 
CTGGTCTGTC AGATCTACTC TGTGGAGGGA AGCAAGTACA TCAAGATCCG CTCTCCCTTC 
CAGATCATAA ACCACTTCTC CATCCCATTC AGAGTGATGG AGGGCTCTAC CAGTTTGGGG 
ATATCACTGC CTACAGAGGA GTTCTGTGTG CCTCTGGACT CCTACAGGTC AGAGCTAAGA 
CTGCAGCCGG TAACCGAGGG AGACGATCAG TACGACTGCT CCGAGGGTTT CAGCTACGAG 
TTGGTGAGCC AGCAGCCCGG TGCACGTGTG CAGCAGACCT GTCATCGCCA TGGTGACAGT 
GCTGGCGTCC TCATGGTCAA CATCGTCCCT CTGAAAGACA CGGTGACATC ACAACACACA 
GGAATGGCTG GGGAGCACTT TGACGAGCCC TACATCCTGC ACCTGTGGCC CTCCGTCCTG 
CTTCGGAACC TTCTGCCTTA CCCCATCACC TATCGCCTAC AGGACAGTGA AGAGCCCACC 
CCCATCACCC TGATGGACGG CCACTCGGCC CAGCTGCACA CCGCCATCAT GGACCAGTCC 
AGCCTGGAAC TCCGCCTCCT CGACTACCTG GGTCAGGATT GGCAGGCCCA CTACGGTCTC 
TGTGGCGACC TGGATGAGAT CACCTTCATT GTGTTCAGCT CTCTGCGCGA TGAAGAGGAG 
GTGGTCAGGT TAGGAGAGAG GGGAGAAGGG AAGAGGGCAG AGCTCGATAT TGCGGTGCAT 
GTGAAGTACG ACGCGGGCCA GACTGAGGTG GCTATCCATT CTCCCTACTG GATGGTGAAC 
AAGACGGGCC GCCTGCTGCA ATACAAGGCC GATGACATCC ACCGCAAACA CGCCATGGAC 
TACGATATGC CTCTGCTCTT CTCCTTCAAG CCACACAACT TTCTTCAGAA CAACAAGGTG 
CGGCTGATGA TCTCGGACAG CGACCTGTCG GATGATTTCT CCCTGGATAC CGTGGGTAGT 
TATGGTGAAG TGAAGTGCAA AGGGAGGCTG AAAGATTACG TGATCGGAGT GAAGATCGAC 
GCCAGCAGTT TCAGCCTGAC CCGTATCGTA AGCTTTGTGC CATACTACCT GTTAGTGAAC 
AGGACCAAAC ATGTGATCTG GGTGGGAGAG GAGGGCCAAG ACGACTGGAC CGAGGCCAGA 
CCACAACAGG CAGCCATCCC GTTTTGGCCT GAGAGGGACT CCAAGAAGCT CAAAGTCAAA 
GTAGAAGGCA GTCGGTCAAC TCCTCAAACC ATGGACTTCA CCAAACCGGA AAACTGCCTG 
CTGCTGCATC TGGACAACAC TCTGGGCGGG GTCATTATGG ATGTGAACCT ATCGGACCAC 
TCGATTATCA TCCGTTTCTC TGACTACCAC GACGGGGCCG CCCCCTTCCT CATCATCAAC 
CACACCCAGG AGCAGACGGT GGAGTTCTAC CAGAGATTTC GGAGAGATTC ATCAGAATGG 
GTGGCAGACG AGCTGCTCGG CCTCTCCTTC TCAGTAGAGG AGACCCAGCC CAGTAGTCAG 
AAGGAGAAGG ACAAGCTAAG CCCCGGGAAG GCAATGTACT ACACCTGGGC TGAGCCCACT 
GGATCGCGCT CTCTGGCCTG GCAGTGTGGC AGCTACAGCG GCGAGCTGAG GAGCGAAGAG 
GACCTGCGGT TGGACGTGAA CGAGGAGGGT AAGCTGTACC TCATCTCGTT CTACGAGGGC 
CTCCAGCGGG TGGCGCTGTT CACAGAGGAC AGACGCATAT ACAAGCTGCT GTGTGAGAGT 
GAGAAGGCCG AGCTGGCCGA ACAAGAGATC ATCCTCTCCC TGCAAGACGT GGGCGTCTCC 
CTGGTCAACA ACAGCAGCCG CCAGGAGGTG TCCTTCATCG GTATTACCAG TTCAGACGTG 
GTGTGGGAGT CCAAGCCAAA GAAGAAGTCT CGCTGGAAAC CCCTGAGTGG AAAAGATGCC 
GAGCTGCTGG AGACCCACTT CAAAGAGTAC ACAGAGAGTG GACCAGTGGA CAACGCCATC 
TTAGACCTGG AGAACAACTT CCAGGTGTCT TTGACCCATA ATGGGCAAGA CATGAGGATG 
ATGCAGCCAT ATGACGCTCC AATCAGGAGG AGCTTCCTGC CAGGGGTGAA GGTGGAGTAC 
AGCGTATCGG CCCGCCAGAG TGCCTACCGT GTCCAGATCA ACCGCATACA GATCCAGAAT 
CAGCTACCTG GAGCAATCTT CCCATATGTC TTCTACCCTA TAAAACTACC TAAGTCTGTC 
ACCATGGACT CAGAGCCCAA ACCTCTGACC GACATCAGCA TTGTTACCAG GGCAGCAGGG 
CACTCTGATA TCTCACGCAT CAAGTATTTC AAGGTGCTAA TCCAGGAGAT GGATCTGAAG 
TTGGACTTGG GCTTCCTTTA TGCCATCTTG GATCTGTTCA CTCCGGAGAA CGCCAGCGTG 
ATGACGTCAG AGCAGCAGGT GGAGTTGTTT GAGAAGGACA TAGAGTATCT GAAGACGGAG 
CTCAACGACG TCTCGGCCAC TGACACATCC CCTATCAGTC TCTATGAGTA CTTCCACATC 
TCTCCTATCA AGCTGCACCT CAGTTTCTCT CTGAGTTCTG GAGGAGAGGA TGGTAACAAG 
GAGAAGAGGG AGACCGAGAT CATCCCTGTC CAGTCCCTCA ATCTGCTGCT GAAAAGCATC 
GGAGCAACCC TCACTGACGT GCAGGATGTC GTCTTCAAAT TGGCCTTCTT CGAGCTGACC 
TTCCAGTTTC GCACTCGCCA GCAACTACAA TGGGAGGTCA TCAGACACTA CTCGAAACAG 
GCGATAAAGC AGATGTATGT GCTGGTGTTG GGCTTGGACG TGCTGGGAAA CCCCTTTGGA 
CTGATCCGAG GTCTGTCGGA GGGAGTGGAG GCTTTCTTCT ACGAGCCCTA TCAGGGAGCT 
ATCCAGGGGC CAGAGGAGTT TGTGGAGGGT ATGGCGCTGG GGGTGAAAGC GCTGGTTGGA 
GGAGCAGTAG GTGGCATTGC GGGCGCTGCC TCCAGGATCA CAGGCGCCAT GGCGAAGGGT 
GTGGCTGCCA TGACAATGGA CGAGGAGTAC CAGCAGAAGA GACGGGAGGC TATGAACAAG 
CAGCCTAGCG GCCTGAGAGA GGGCCTGACC CGCGGAGGCA AAGGACTGGT GTCTGGCTTT 
GTCAGTGGGA TCACAGGGAT TGTCACCAAG CCCATTAAAG GTGCTCAGAA GGAGGGCGCG 
GCGGGCTTCT TTAAGGGTGT GGGCAAGGGG CTAGTGGGTG CTGTGGCCAG ACCCACTGGA 
GGCATCATCG ACATGGCCAG CAGCACCTTC CAGGGCATCA AGAGGGCAGC TGAGACATCC 
CAGGATGTGG AGAGTCTGCG TCCGCCTCGC TTCATCCATG AGGACGGGGT CATCCGACCA 
TACAAGGAAA GAGAGGGCAT CGGCAGCCAG ATGCTACAGA AAAGGTTGGC CTTCACTGAA 
TGGAGTAGGA CTGGCAATGA TGATGATGAT GATGATGATG ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021476715.1
CDS	274..9801
Translation	MVFESVVVDVLNRFLGDYVVNLDSSQLKLGIWGGDAVLRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDGVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEETKQKAAEQENPALEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPNCPFSFGVSLQNLSLQTADENWMPCLLDEKAKLFFKLVQLDNLFAYWNVNSMLYSNHMPDEALRCLQDSIPGGSTPMNHDFIFRPITADAKLRMNPRSDVDFSSPKVDLVVNLREVAVELNRPQYISILELLGSVDMMSRNLPYRKYRPQVHVHTNAYLWWQYVITGIMEVDVKPRLHMWSWQHIRCHRQTVKQYRELYKAKITSKKPTEKQLRELEEPERTLDIFNITLARQQAEMEASKAGLRIFRPGVKVEEEESQGWLGWMWSGWSGDCGAEAKEVKTGAFDELMTSAEKAKLYTAIGYSEIAVNHNLPKNFEDMKINFHMERMSVSMKDNKDQNEILKLTVEDLKSMLTQRPGAQAIKLSAQLSLFEVTGLASNRPAPTLLSSRKAATVTGTPLLDILFETNPLDESADQRLRVESQPLEIIYDAVTVNSMSAFFSPPDDLQLDDLTNATLMKLEQFRDRTSTGLMYVIETQKVLDLNVNLMASYVIVPQTGFHDSTQNLMLLDLGHFKVSSLSREGLPQLSVGRSTIEDIMSRAYDSFDVQLSSLQFLYSKPDGDWKKARKLRQSALHILEPVDVKVVFSRAMVVTDSRMPKFKISGELPLLSLRISDDKVRGVLELIDSIPLPESRPAPRSTNAHSTSKALKQSYTPQLTPRKPTMAELRNSLIWESGSEEDLFYDAPSSPIGDRPFFPDNMPSPLQRSDSVKRRGLVKKDPKKNMTEFLMTFEISKLSVQLCRLSGGEEVTVLHLDIEGLGTELKLRTFDMTSYTFLREICLECPEYMDSEEKKVHLITTLDNTEDDLLTLEYTKADKNGPEFKSEYKNTEQSVKVNFSSLDIHLHTEALLNAMNFLISLLPPTKEVAPSQEELPTIPEEDEGEGEEGEKKEGSAVAKKTSSKKSKFEDVVNLHIRADLNCLKVFIRGQSHRIAEIRIEGLVSEVLMRKKAVEVLANLKNIVILDCDKEALYKQCVSIADEEVFAFRMVNHTEATEGDAYLDMGKVDTSVTLTVGCIQVIFLNKFVSSILAFINNFQVAKEALAEVTVQAAEKAATGVMELAERSTRISLDVDFNAPVVFLPQSSSSANVIVADLGMVTVKNRFAMVPSQMHVKIPPVMDTMTVGLSDLKMYRTTFEEGRFQGEIQLLKPVNLDLSIQRNLSASWYHSIPDIEITAHLKPMNLILGQDDMTVVLRTLSGNLSETSDSASPPPPPRTVDRSDTASTKGSQASGGTGTNPTSGNTVVTAAVVETQKSTKLKSTLKLDFKFDSMTLVLYSPNGTEVQVLDSRDEQLKLAEFTLGTISTSVHMFSDGSMKASVKLTDCLLDDKRCRVKKITARMMAMRPGAERNVMVEVNYKQGRDGTSLDTVVQDVYLCASMEFLLTVADIFLKASQQGFSSATPKNSSSTKPTAAAASKESAPVGVVAKSEINVLVKNPEIVFVADLTRADAPALVMTTECELVMKSDLDGQLMTAVVKDLKVVACPFLKEKRNKETTVLQPCQVFFKSTQSSTSPQAMEVCINALTLKVSPIIINTVITIQSVLSPAGAETPQSELESPVPPDLWERRSWRDLKLWFLEEEGKDEDAQSLVPLIPQGESLQLAINSICLTLEAGVGHRTVPMLLAKSSFHGDVKNWSTLINLHSQLNLEVHYFNEVMGVWEPLLEPLEDETRDGFRPWTLGLKMKKKPVKYTGISEEVDYSIPDYKTVISISSKDQLNITLSKCGLGMLSNLGTAFAEAAKQASDSFQKDEAPFVVKNRLGLTVSVVHSELFSPLGREATARRVELQDGESLNMDYRRTTTHSDQFSAMTSLVAKDYYIQPTPVGHTSTGMIPLIKVGRGMYSVMHKDSGVTRFLVCQIYSVEGSKYIKIRSPFQIINHFSIPFRVMEGSTSLGISLPTEEFCVPLDSYRSELRLQPVTEGDDQYDCSEGFSYELVSQQPGARVQQTCHRHGDSAGVLMVNIVPLKDTVTSQHTGMAGEHFDEPYILHLWPSVLLRNLLPYPITYRLQDSEEPTPITLMDGHSAQLHTAIMDQSSLELRLLDYLGQDWQAHYGLCGDLDEITFIVFSSLRDEEEVVRLGERGEGKRAELDIAVHVKYDAGQTEVAIHSPYWMVNKTGRLLQYKADDIHRKHAMDYDMPLLFSFKPHNFLQNNKVRLMISDSDLSDDFSLDTVGSYGEVKCKGRLKDYVIGVKIDASSFSLTRIVSFVPYYLLVNRTKHVIWVGEEGQDDWTEARPQQAAIPFWPERDSKKLKVKVEGSRSTPQTMDFTKPENCLLLHLDNTLGGVIMDVNLSDHSIIIRFSDYHDGAAPFLIINHTQEQTVEFYQRFRRDSSEWVADELLGLSFSVEETQPSSQKEKDKLSPGKAMYYTWAEPTGSRSLAWQCGSYSGELRSEEDLRLDVNEEGKLYLISFYEGLQRVALFTEDRRIYKLLCESEKAELAEQEIILSLQDVGVSLVNNSSRQEVSFIGITSSDVVWESKPKKKSRWKPLSGKDAELLETHFKEYTESGPVDNAILDLENNFQVSLTHNGQDMRMMQPYDAPIRRSFLPGVKVEYSVSARQSAYRVQINRIQIQNQLPGAIFPYVFYPIKLPKSVTMDSEPKPLTDISIVTRAAGHSDISRIKYFKVLIQEMDLKLDLGFLYAILDLFTPENASVMTSEQQVELFEKDIEYLKTELNDVSATDTSPISLYEYFHISPIKLHLSFSLSSGGEDGNKEKRETEIIPVQSLNLLLKSIGATLTDVQDVVFKLAFFELTFQFRTRQQLQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGIAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRAAETSQDVESLRPPRFIHEDGVIRPYKEREGIGSQMLQKRLAFTEWSRTGNDDDDDDDDS

Target ORF information:

RefSeq Version	XM_021621040.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621040.1

ORF Insert Sequence:

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGT 
ACTGGTACTA ACCCCACCAG CGGCAACACG GTGGTAACTG CTGCAGTGGT GGAGACCCAG 
AAGAGCACCA AGCTGAAGAG CACACTCAAA CTGGACTTCA AGTTTGACTC CATGACCCTG 
GTCCTGTACA GCCCCAATGG GACTGAGGTG CAGGTGTTGG ACTCGAGGGA TGAGCAGCTG 
AAGCTGGCTG AGTTTACCCT GGGGACTATC TCTACATCTG TCCACATGTT CTCAGACGGA 
TCTATGAAGG CCTCCGTCAA ACTCACAGAC TGCCTGCTAG ACGACAAAAG ATGCAGGGTC 
AAGAAGATCA CTGCCAGGAT GATGGCGATG CGTCCGGGCG CTGAGAGGAA CGTGATGGTG 
GAGGTGAACT ACAAGCAGGG CCGCGACGGC ACCTCTTTGG ACACGGTGGT GCAGGACGTG 
TACCTCTGTG CCAGCATGGA GTTCCTGCTC ACTGTGGCCG ACATCTTCCT CAAAGCCAGC 
CAGCAGGGCT TCTCCTCCGC CACCCCCAAG AACAGCAGTA GCACTAAACC CACTGCAGCC 
GCAGCCTCCA AGGAATCTGC TCCAGTGGGG GTGGTGGCAA AGTCAGAGAT TAATGTTCTG 
GTGAAGAACC CCGAGATTGT GTTTGTGGCC GACCTGACAC GTGCCGACGC CCCGGCCCTG 
GTCATGACCA CGGAGTGTGA GCTGGTGATG AAGAGCGACC TGGACGGTCA GCTGATGACT 
GCGGTGGTCA AGGACCTGAA GGTGGTGGCC TGCCCCTTCC TGAAGGAGAA GAGAAACAAG 
GAGACCACTG TGCTGCAGCC CTGCCAAGTG TTCTTCAAGA GCACACAGTC CTCCACCTCC 
CCGCAGGCCA TGGAGGTCTG CATCAACGCA CTCACACTCA AGGTGTCGCC CATCATTATC 
AACACAGTGA TTACCATTCA GTCGGTGCTG AGCCCGGCCG GTGCCGAGAC ACCCCAGTCT 
GAGCTAGAGA GCCCCGTGCC CCCAGACCTG TGGGAGCGCC GGTCCTGGAG GGACCTCAAG 
CTGTGGTTCC TGGAGGAGGA GGGTAAAGAC GAGGATGCCC AGTCCCTGGT CCCGCTGATT 
CCCCAGGGCG AGTCCCTGCA GTTGGCCATC AACTCCATCT GCCTGACACT GGAGGCAGGG 
GTGGGCCACC GCACCGTCCC CATGCTCCTG GCCAAGTCCT CCTTCCACGG TGACGTCAAG 
AACTGGAGCA CCCTCATCAA CCTGCACAGC CAGCTCAACC TGGAGGTTCA CTACTTCAAC 
GAGGTGATGG GGGTGTGGGA GCCACTGCTA GAGCCCCTAG AAGACGAGAC CCGAGATGGA 
TTCAGACCCT GGACCCTGGG ATTAAAGATG AAGAAGAAGC CAGTGAAGTA CACAGGTATT 
TCAGAAGAAG TGGACTACAG CATCCCTGAC TACAAGACTG TCATCAGCAT CAGCAGCAAG 
GACCAACTTA ATATCACCCT CTCCAAGTGT GGACTGGGCA TGCTCAGTAA CCTGGGCACG 
GCCTTTGCGG AGGCGGCCAA GCAGGCATCG GATAGTTTCC AGAAGGACGA GGCTCCGTTC 
GTCGTGAAGA ACCGTCTAGG TCTGACTGTG TCGGTGGTGC ACAGTGAGCT GTTCAGCCCC 
CTGGGTCGCG AGGCCACGGC GCGCCGGGTA GAGCTGCAGG ACGGAGAGAG CCTCAACATG 
GACTACCGTC GCACCACCAC CCACTCAGAC CAGTTCTCTG CCATGACTTC ATTGGTGGCC 
AAGGACTACT ACATACAGCC CACTCCGGTG GGCCACACTT CAACAGGCAT GATCCCGTTG 
ATCAAAGTGG GGCGTGGCAT GTACAGTGTC ATGCACAAAG ACTCTGGGGT CACCCGTTTC 
CTGGTCTGTC AGATCTACTC TGTGGAGGGA AGCAAGTACA TCAAGATCCG CTCTCCCTTC 
CAGATCATAA ACCACTTCTC CATCCCATTC AGAGTGATGG AGGGCTCTAC CAGTTTGGGG 
ATATCACTGC CTACAGAGGA GTTCTGTGTG CCTCTGGACT CCTACAGGTC AGAGCTAAGA 
CTGCAGCCGG TAACCGAGGG AGACGATCAG TACGACTGCT CCGAGGGTTT CAGCTACGAG 
TTGGTGAGCC AGCAGCCCGG TGCACGTGTG CAGCAGACCT GTCATCGCCA TGGTGACAGT 
GCTGGCGTCC TCATGGTCAA CATCGTCCCT CTGAAAGACA CGGTGACATC ACAACACACA 
GGAATGGCTG GGGAGCACTT TGACGAGCCC TACATCCTGC ACCTGTGGCC CTCCGTCCTG 
CTTCGGAACC TTCTGCCTTA CCCCATCACC TATCGCCTAC AGGACAGTGA AGAGCCCACC 
CCCATCACCC TGATGGACGG CCACTCGGCC CAGCTGCACA CCGCCATCAT GGACCAGTCC 
AGCCTGGAAC TCCGCCTCCT CGACTACCTG GGTCAGGATT GGCAGGCCCA CTACGGTCTC 
TGTGGCGACC TGGATGAGAT CACCTTCATT GTGTTCAGCT CTCTGCGCGA TGAAGAGGAG 
GTGGTCAGGT TAGGAGAGAG GGGAGAAGGG AAGAGGGCAG AGCTCGATAT TGCGGTGCAT 
GTGAAGTACG ACGCGGGCCA GACTGAGGTG GCTATCCATT CTCCCTACTG GATGGTGAAC 
AAGACGGGCC GCCTGCTGCA ATACAAGGCC GATGACATCC ACCGCAAACA CGCCATGGAC 
TACGATATGC CTCTGCTCTT CTCCTTCAAG CCACACAACT TTCTTCAGAA CAACAAGGTG 
CGGCTGATGA TCTCGGACAG CGACCTGTCG GATGATTTCT CCCTGGATAC CGTGGGTAGT 
TATGGTGAAG TGAAGTGCAA AGGGAGGCTG AAAGATTACG TGATCGGAGT GAAGATCGAC 
GCCAGCAGTT TCAGCCTGAC CCGTATCGTA AGCTTTGTGC CATACTACCT GTTAGTGAAC 
AGGACCAAAC ATGTGATCTG GGTGGGAGAG GAGGGCCAAG ACGACTGGAC CGAGGCCAGA 
CCACAACAGG CAGCCATCCC GTTTTGGCCT GAGAGGGACT CCAAGAAGCT CAAAGTCAAA 
GTAGAAGGCA GTCGGTCAAC TCCTCAAACC ATGGACTTCA CCAAACCGGA AAACTGCCTG 
CTGCTGCATC TGGACAACAC TCTGGGCGGG GTCATTATGG ATGTGAACCT ATCGGACCAC 
TCGATTATCA TCCGTTTCTC TGACTACCAC GACGGGGCCG CCCCCTTCCT CATCATCAAC 
CACACCCAGG AGCAGACGGT GGAGTTCTAC CAGAGATTTC GGAGAGATTC ATCAGAATGG 
GTGGCAGACG AGCTGCTCGG CCTCTCCTTC TCAGTAGAGG AGACCCAGCC CAGTAGTCAG 
AAGGAGAAGG ACAAGCTAAG CCCCGGGAAG GCAATGTACT ACACCTGGGC TGAGCCCACT 
GGATCGCGCT CTCTGGCCTG GCAGTGTGGC AGCTACAGCG GCGAGCTGAG GAGCGAAGAG 
GACCTGCGGT TGGACGTGAA CGAGGAGGGT AAGCTGTACC TCATCTCGTT CTACGAGGGC 
CTCCAGCGGG TGGCGCTGTT CACAGAGGAC AGACGCATAT ACAAGCTGCT GTGTGAGAGT 
GAGAAGGCCG AGCTGGCCGA ACAAGAGATC ATCCTCTCCC TGCAAGACGT GGGCGTCTCC 
CTGGTCAACA ACAGCAGCCG CCAGGAGGTG TCCTTCATCG GTATTACCAG TTCAGACGTG 
GTGTGGGAGT CCAAGCCAAA GAAGAAGTCT CGCTGGAAAC CCCTGAGTGG AAAAGATGCC 
GAGCTGCTGG AGACCCACTT CAAAGAGTAC ACAGAGAGTG GACCAGTGGA CAACGCCATC 
TTAGACCTGG AGAACAACTT CCAGGTGTCT TTGACCCATA ATGGGCAAGA CATGAGGATG 
ATGCAGCCAT ATGACGCTCC AATCAGGAGG AGCTTCCTGC CAGGGGTGAA GGTGGAGTAC 
AGCGTATCGG CCCGCCAGAG TGCCTACCGT GTCCAGATCA ACCGCATACA GATCCAGAAT 
CAGCTACCTG GAGCAATCTT CCCATATGTC TTCTACCCTA TAAAACTACC TAAGTCTGTC 
ACCATGGACT CAGAGCCCAA ACCTCTGACC GACATCAGCA TTGTTACCAG GGCAGCAGGG 
CACTCTGATA TCTCACGCAT CAAGTATTTC AAGGTGCTAA TCCAGGAGAT GGATCTGAAG 
TTGGACTTGG GCTTCCTTTA TGCCATCTTG GATCTGTTCA CTCCGGAGAA CGCCAGCGTG 
ATGACGTCAG AGCAGCAGGT GGAGTTGTTT GAGAAGGACA TAGAGTATCT GAAGACGGAG 
CTCAACGACG TCTCGGCCAC TGACACATCC CCTATCAGTC TCTATGAGTA CTTCCACATC 
TCTCCTATCA AGCTGCACCT CAGTTTCTCT CTGAGTTCTG GAGGAGAGGA TGGTAACAAG 
GAGAAGAGGG AGACCGAGAT CATCCCTGTC CAGTCCCTCA ATCTGCTGCT GAAAAGCATC 
GGAGCAACCC TCACTGACGT GCAGGATGTC GTCTTCAAAT TGGCCTTCTT CGAGCTGACC 
TTCCAGTTTC GCACTCGCCA GCAACTACAA TGGGAGGTCA TCAGACACTA CTCGAAACAG 
GCGATAAAGC AGATGTATGT GCTGGTGTTG GGCTTGGACG TGCTGGGAAA CCCCTTTGGA 
CTGATCCGAG GTCTGTCGGA GGGAGTGGAG GCTTTCTTCT ACGAGCCCTA TCAGGGAGCT 
ATCCAGGGGC CAGAGGAGTT TGTGGAGGGT ATGGCGCTGG GGGTGAAAGC GCTGGTTGGA 
GGAGCAGTAG GTGGCATTGC GGGCGCTGCC TCCAGGATCA CAGGCGCCAT GGCGAAGGGT 
GTGGCTGCCA TGACAATGGA CGAGGAGTAC CAGCAGAAGA GACGGGAGGC TATGAACAAG 
CAGCCTAGCG GCCTGAGAGA GGGCCTGACC CGCGGAGGCA AAGGACTGGT GTCTGGCTTT 
GTCAGTGGGA TCACAGGGAT TGTCACCAAG CCCATTAAAG GTGCTCAGAA GGAGGGCGCG 
GCGGGCTTCT TTAAGGGTGT GGGCAAGGGG CTAGTGGGTG CTGTGGCCAG ACCCACTGGA 
GGCATCATCG ACATGGCCAG CAGCACCTTC CAGGGCATCA AGAGGGCAGC TGAGACATCC 
CAGGATGTGG AGAGTCTGCG TCCGCCTCGC TTCATCCATG AGGACGGGGT CATCCGACCA 
TACAAGGAAA GAGAGGGCAT CGGCAGCCAG ATGCTACAGA AAAGGTTGGC CTTCACTGAA 
TGGAGTAGGA CTGGCAATGA TGATGATGAT GATGATGATG ACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps13a ( XM_021621041.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021476716.1 Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X7, mRNA.

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CloneID	OOf56509
Clone ID Related Accession (Same CDS sequence)	XM_021621041.1
Accession Version	XM_021621041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	vacuolar protein sorting-associated protein 13A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGC 
AACACGGTGG TAACTGCTGC AGTGGTGGAG ACCCAGAAGA GCACCAAGCT GAAGAGCACA 
CTCAAACTGG ACTTCAAGTT TGACTCCATG ACCCTGGTCC TGTACAGCCC CAATGGGACT 
GAGGTGCAGG TGTTGGACTC GAGGGATGAG CAGCTGAAGC TGGCTGAGTT TACCCTGGGG 
ACTATCTCTA CATCTGTCCA CATGTTCTCA GACGGATCTA TGAAGGCCTC CGTCAAACTC 
ACAGACTGCC TGCTAGACGA CAAAAGATGC AGGGTCAAGA AGATCACTGC CAGGATGATG 
GCGATGCGTC CGGGCGCTGA GAGGAACGTG ATGGTGGAGG TGAACTACAA GCAGGGCCGC 
GACGGCACCT CTTTGGACAC GGTGGTGCAG GACGTGTACC TCTGTGCCAG CATGGAGTTC 
CTGCTCACTG TGGCCGACAT CTTCCTCAAA GCCAGCCAGC AGGGCTTCTC CTCCGCCACC 
CCCAAGAACA GCAGTAGCAC TAAACCCACT GCAGCCGCAG CCTCCAAGGA ATCTGCTCCA 
GTGGGGGTGG TGGCAAAGTC AGAGATTAAT GTTCTGGTGA AGAACCCCGA GATTGTGTTT 
GTGGCCGACC TGACACGTGC CGACGCCCCG GCCCTGGTCA TGACCACGGA GTGTGAGCTG 
GTGATGAAGA GCGACCTGGA CGGTCAGCTG ATGACTGCGG TGGTCAAGGA CCTGAAGGTG 
GTGGCCTGCC CCTTCCTGAA GGAGAAGAGA AACAAGGAGA CCACTGTGCT GCAGCCCTGC 
CAAGTGTTCT TCAAGAGCAC ACAGTCCTCC ACCTCCCCGC AGGCCATGGA GGTCTGCATC 
AACGCACTCA CACTCAAGGT GTCGCCCATC ATTATCAACA CAGTGATTAC CATTCAGTCG 
GTGCTGAGCC CGGCCGGTGC CGAGACACCC CAGTCTGAGC TAGAGAGCCC CGTGCCCCCA 
GACCTGTGGG AGCGCCGGTC CTGGAGGGAC CTCAAGCTGT GGTTCCTGGA GGAGGAGGGT 
AAAGACGAGG ATGCCCAGTC CCTGGTCCCG CTGATTCCCC AGGGCGAGTC CCTGCAGTTG 
GCCATCAACT CCATCTGCCT GACACTGGAG GCAGGGGTGG GCCACCGCAC CGTCCCCATG 
CTCCTGGCCA AGTCCTCCTT CCACGGTGAC GTCAAGAACT GGAGCACCCT CATCAACCTG 
CACAGCCAGC TCAACCTGGA GGTTCACTAC TTCAACGAGG TGATGGGGGT GTGGGAGCCA 
CTGCTAGAGC CCCTAGAAGA CGAGACCCGA GATGGATTCA GACCCTGGAC CCTGGGATTA 
AAGATGAAGA AGAAGCCAGT GAAGTACACA GGTATTTCAG AAGAAGTGGA CTACAGCATC 
CCTGACTACA AGACTGTCAT CAGCATCAGC AGCAAGGACC AACTTAATAT CACCCTCTCC 
AAGTGTGGAC TGGGCATGCT CAGTAACCTG GGCACGGCCT TTGCGGAGGC GGCCAAGCAG 
GCATCGGATA GTTTCCAGAA GGACGAGGCT CCGTTCGTCG TGAAGAACCG TCTAGGTCTG 
ACTGTGTCGG TGGTGCACAG TGAGCTGTTC AGCCCCCTGG GTCGCGAGGC CACGGCGCGC 
CGGGTAGAGC TGCAGGACGG AGAGAGCCTC AACATGGACT ACCGTCGCAC CACCACCCAC 
TCAGACCAGT TCTCTGCCAT GACTTCATTG GTGGCCAAGG ACTACTACAT ACAGCCCACT 
CCGGTGGGCC ACACTTCAAC AGGCATGATC CCGTTGATCA AAGTGGGGCG TGGCATGTAC 
AGTGTCATGC ACAAAGACTC TGGGGTCACC CGTTTCCTGG TCTGTCAGAT CTACTCTGTG 
GAGGGAAGCA AGTACATCAA GATCCGCTCT CCCTTCCAGA TCATAAACCA CTTCTCCATC 
CCATTCAGAG TGATGGAGGG CTCTACCAGT TTGGGGATAT CACTGCCTAC AGAGGAGTTC 
TGTGTGCCTC TGGACTCCTA CAGGTCAGAG CTAAGACTGC AGCCGGTAAC CGAGGGAGAC 
GATCAGTACG ACTGCTCCGA GGGTTTCAGC TACGAGTTGG TGAGCCAGCA GCCCGGTGCA 
CGTGTGCAGC AGACCTGTCA TCGCCATGGT GACAGTGCTG GCGTCCTCAT GGTCAACATC 
GTCCCTCTGA AAGACACGGT GACATCACAA CACACAGGAA TGGCTGGGGA GCACTTTGAC 
GAGCCCTACA TCCTGCACCT GTGGCCCTCC GTCCTGCTTC GGAACCTTCT GCCTTACCCC 
ATCACCTATC GCCTACAGGA CAGTGAAGAG CCCACCCCCA TCACCCTGAT GGACGGCCAC 
TCGGCCCAGC TGCACACCGC CATCATGGAC CAGTCCAGCC TGGAACTCCG CCTCCTCGAC 
TACCTGGGTC AGGATTGGCA GGCCCACTAC GGTCTCTGTG GCGACCTGGA TGAGATCACC 
TTCATTGTGT TCAGCTCTCT GCGCGATGAA GAGGAGGTGG TCAGGTTAGG AGAGAGGGGA 
GAAGGGAAGA GGGCAGAGCT CGATATTGCG GTGCATGTGA AGTACGACGC GGGCCAGACT 
GAGGTGGCTA TCCATTCTCC CTACTGGATG GTGAACAAGA CGGGCCGCCT GCTGCAATAC 
AAGGCCGATG ACATCCACCG CAAACACGCC ATGGACTACG ATATGCCTCT GCTCTTCTCC 
TTCAAGCCAC ACAACTTTCT TCAGAACAAC AAGGTGCGGC TGATGATCTC GGACAGCGAC 
CTGTCGGATG ATTTCTCCCT GGATACCGTG GGTAGTTATG GTGAAGTGAA GTGCAAAGGG 
AGGCTGAAAG ATTACGTGAT CGGAGTGAAG ATCGACGCCA GCAGTTTCAG CCTGACCCGT 
ATCGTAAGCT TTGTGCCATA CTACCTGTTA GTGAACAGGA CCAAACATGT GATCTGGGTG 
GGAGAGGAGG GCCAAGACGA CTGGACCGAG GCCAGACCAC AACAGGCAGC CATCCCGTTT 
TGGCCTGAGA GGGACTCCAA GAAGCTCAAA GTCAAAGTAG AAGGCAGTCG GTCAACTCCT 
CAAACCATGG ACTTCACCAA ACCGGAAAAC TGCCTGCTGC TGCATCTGGA CAACACTCTG 
GGCGGGGTCA TTATGGATGT GAACCTATCG GACCACTCGA TTATCATCCG TTTCTCTGAC 
TACCACGACG GGGCCGCCCC CTTCCTCATC ATCAACCACA CCCAGGAGCA GACGGTGGAG 
TTCTACCAGA GTAGTCAGAA GGAGAAGGAC AAGCTAAGCC CCGGGAAGGC AATGTACTAC 
ACCTGGGCTG AGCCCACTGG ATCGCGCTCT CTGGCCTGGC AGTGTGGCAG CTACAGCGGC 
GAGCTGAGGA GCGAAGAGGA CCTGCGGTTG GACGTGAACG AGGAGGGTAA GCTGTACCTC 
ATCTCGTTCT ACGAGGGCCT CCAGCGGGTG GCGCTGTTCA CAGAGGACAG ACGCATATAC 
AAGCTGCTGT GTGAGAGTGA GAAGGCCGAG CTGGCCGAAC AAGAGATCAT CCTCTCCCTG 
CAAGACGTGG GCGTCTCCCT GGTCAACAAC AGCAGCCGCC AGGAGGTGTC CTTCATCGGT 
ATTACCAGTT CAGACGTGGT GTGGGAGTCC AAGCCAAAGA AGAAGTCTCG CTGGAAACCC 
CTGAGTGGAA AAGATGCCGA GCTGCTGGAG ACCCACTTCA AAGAGTACAC AGAGAGTGGA 
CCAGTGGACA ACGCCATCTT AGACCTGGAG AACAACTTCC AGGTGTCTTT GACCCATAAT 
GGGCAAGACA TGAGGATGAT GCAGCCATAT GACGCTCCAA TCAGGAGGAG CTTCCTGCCA 
GGGGTGAAGG TGGAGTACAG CGTATCGGCC CGCCAGAGTG CCTACCGTGT CCAGATCAAC 
CGCATACAGA TCCAGAATCA GCTACCTGGA GCAATCTTCC CATATGTCTT CTACCCTATA 
AAACTACCTA AGTCTGTCAC CATGGACTCA GAGCCCAAAC CTCTGACCGA CATCAGCATT 
GTTACCAGGG CAGCAGGGCA CTCTGATATC TCACGCATCA AGTATTTCAA GGTGCTAATC 
CAGGAGATGG ATCTGAAGTT GGACTTGGGC TTCCTTTATG CCATCTTGGA TCTGTTCACT 
CCGGAGAACG CCAGCGTGAT GACGTCAGAG CAGCAGGTGG AGTTGTTTGA GAAGGACATA 
GAGTATCTGA AGACGGAGCT CAACGACGTC TCGGCCACTG ACACATCCCC TATCAGTCTC 
TATGAGTACT TCCACATCTC TCCTATCAAG CTGCACCTCA GTTTCTCTCT GAGTTCTGGA 
GGAGAGGATG GTAACAAGGA GAAGAGGGAG ACCGAGATCA TCCCTGTCCA GTCCCTCAAT 
CTGCTGCTGA AAAGCATCGG AGCAACCCTC ACTGACGTGC AGGATGTCGT CTTCAAATTG 
GCCTTCTTCG AGCTGACCTT CCAGTTTCGC ACTCGCCAGC AACTACAATG GGAGGTCATC 
AGACACTACT CGAAACAGGC GATAAAGCAG ATGTATGTGC TGGTGTTGGG CTTGGACGTG 
CTGGGAAACC CCTTTGGACT GATCCGAGGT CTGTCGGAGG GAGTGGAGGC TTTCTTCTAC 
GAGCCCTATC AGGGAGCTAT CCAGGGGCCA GAGGAGTTTG TGGAGGGTAT GGCGCTGGGG 
GTGAAAGCGC TGGTTGGAGG AGCAGTAGGT GGCATTGCGG GCGCTGCCTC CAGGATCACA 
GGCGCCATGG CGAAGGGTGT GGCTGCCATG ACAATGGACG AGGAGTACCA GCAGAAGAGA 
CGGGAGGCTA TGAACAAGCA GCCTAGCGGC CTGAGAGAGG GCCTGACCCG CGGAGGCAAA 
GGACTGGTGT CTGGCTTTGT CAGTGGGATC ACAGGGATTG TCACCAAGCC CATTAAAGGT 
GCTCAGAAGG AGGGCGCGGC GGGCTTCTTT AAGGGTGTGG GCAAGGGGCT AGTGGGTGCT 
GTGGCCAGAC CCACTGGAGG CATCATCGAC ATGGCCAGCA GCACCTTCCA GGGCATCAAG 
AGGGCAGCTG AGACATCCCA GGATGTGGAG AGTCTGCGTC CGCCTCGCTT CATCCATGAG 
GACGGGGTCA TCCGACCATA CAAGGAAAGA GAGGGCATCG GCAGCCAGAT GCTACAGAAA 
ATTGAAAACG GAAGGTTCGC CAAGTACAGA TACTTTGCGC ACGCCAAAGT GAACGAGTCC 
GATTTCCTGA TGATCACCAA AAGGGGAATC TTTTTCGTGA CCAAAGGTAC ATTTGGGCAG 
CTGACCTGCG AGTGGCAGTA TCTGTTCGAC GAGTTCACCA AGGAGCCCAT GATCGTGGAG 
GGGAGGCGGC TTCGGATCGA GGCCAAGGAG AGAGTGAAGT CTGTGTTTCA TGCCAAAGAG 
TTTGGGAAGA TAATCAACTT CAGAAGTCCA GAAATAGCCA AGTGGGTTCT TACCAAGCTT 
GAAGACGCCA GGGAGAGTCT ACACAAATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021476716.1
CDS	274..9966
Translation	MVFESVVVDVLNRFLGDYVVNLDSSQLKLGIWGGDAVLRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDGVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEETKQKAAEQENPALEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPNCPFSFGVSLQNLSLQTADENWMPCLLDEKAKLFFKLVQLDNLFAYWNVNSMLYSNHMPDEALRCLQDSIPGGSTPMNHDFIFRPITADAKLRMNPRSDVDFSSPKVDLVVNLREVAVELNRPQYISILELLGSVDMMSRNLPYRKYRPQVHVHTNAYLWWQYVITGIMEVDVKPRLHMWSWQHIRCHRQTVKQYRELYKAKITSKKPTEKQLRELEEPERTLDIFNITLARQQAEMEASKAGLRIFRPGVKVEEEESQGWLGWMWSGWSGDCGAEAKEVKTGAFDELMTSAEKAKLYTAIGYSEIAVNHNLPKNFEDMKINFHMERMSVSMKDNKDQNEILKLTVEDLKSMLTQRPGAQAIKLSAQLSLFEVTGLASNRPAPTLLSSRKAATVTGTPLLDILFETNPLDESADQRLRVESQPLEIIYDAVTVNSMSAFFSPPDDLQLDDLTNATLMKLEQFRDRTSTGLMYVIETQKVLDLNVNLMASYVIVPQTGFHDSTQNLMLLDLGHFKVSSLSREGLPQLSVGRSTIEDIMSRAYDSFDVQLSSLQFLYSKPDGDWKKARKLRQSALHILEPVDVKVVFSRAMVVTDSRMPKFKISGELPLLSLRISDDKVRGVLELIDSIPLPESRPAPRSTNAHSTSKALKQSYTPQLTPRKPTMAELRNSLIWESGSEEDLFYDAPSSPIGDRPFFPDNMPSPLQRSDSVKRRGLVKKDPKKNMTEFLMTFEISKLSVQLCRLSGGEEVTVLHLDIEGLGTELKLRTFDMTSYTFLREICLECPEYMDSEEKKVHLITTLDNTEDDLLTLEYTKADKNGPEFKSEYKNTEQSVKVNFSSLDIHLHTEALLNAMNFLISLLPPTKEVAPSQEELPTIPEEDEGEGEEGEKKEGSAVAKKTSSKKSKFEDVVNLHIRADLNCLKVFIRGQSHRIAEIRIEGLVSEVLMRKKAVEVLANLKNIVILDCDKEALYKQCVSIADEEVFAFRMVNHTEATEGDAYLDMGKVDTSVTLTVGCIQVIFLNKFVSSILAFINNFQVAKEALAEVTVQAAEKAATGVMELAERSTRISLDVDFNAPVVFLPQSSSSANVIVADLGMVTVKNRFAMVPSQMHVKIPPVMDTMTVGLSDLKMYRTTFEEGRFQGEIQLLKPVNLDLSIQRNLSASWYHSIPDIEITAHLKPMNLILGQDDMTVVLRTLSGNLSETSDSASPPPPPRTVDRSDTASTKGSQASGGNTVVTAAVVETQKSTKLKSTLKLDFKFDSMTLVLYSPNGTEVQVLDSRDEQLKLAEFTLGTISTSVHMFSDGSMKASVKLTDCLLDDKRCRVKKITARMMAMRPGAERNVMVEVNYKQGRDGTSLDTVVQDVYLCASMEFLLTVADIFLKASQQGFSSATPKNSSSTKPTAAAASKESAPVGVVAKSEINVLVKNPEIVFVADLTRADAPALVMTTECELVMKSDLDGQLMTAVVKDLKVVACPFLKEKRNKETTVLQPCQVFFKSTQSSTSPQAMEVCINALTLKVSPIIINTVITIQSVLSPAGAETPQSELESPVPPDLWERRSWRDLKLWFLEEEGKDEDAQSLVPLIPQGESLQLAINSICLTLEAGVGHRTVPMLLAKSSFHGDVKNWSTLINLHSQLNLEVHYFNEVMGVWEPLLEPLEDETRDGFRPWTLGLKMKKKPVKYTGISEEVDYSIPDYKTVISISSKDQLNITLSKCGLGMLSNLGTAFAEAAKQASDSFQKDEAPFVVKNRLGLTVSVVHSELFSPLGREATARRVELQDGESLNMDYRRTTTHSDQFSAMTSLVAKDYYIQPTPVGHTSTGMIPLIKVGRGMYSVMHKDSGVTRFLVCQIYSVEGSKYIKIRSPFQIINHFSIPFRVMEGSTSLGISLPTEEFCVPLDSYRSELRLQPVTEGDDQYDCSEGFSYELVSQQPGARVQQTCHRHGDSAGVLMVNIVPLKDTVTSQHTGMAGEHFDEPYILHLWPSVLLRNLLPYPITYRLQDSEEPTPITLMDGHSAQLHTAIMDQSSLELRLLDYLGQDWQAHYGLCGDLDEITFIVFSSLRDEEEVVRLGERGEGKRAELDIAVHVKYDAGQTEVAIHSPYWMVNKTGRLLQYKADDIHRKHAMDYDMPLLFSFKPHNFLQNNKVRLMISDSDLSDDFSLDTVGSYGEVKCKGRLKDYVIGVKIDASSFSLTRIVSFVPYYLLVNRTKHVIWVGEEGQDDWTEARPQQAAIPFWPERDSKKLKVKVEGSRSTPQTMDFTKPENCLLLHLDNTLGGVIMDVNLSDHSIIIRFSDYHDGAAPFLIINHTQEQTVEFYQSSQKEKDKLSPGKAMYYTWAEPTGSRSLAWQCGSYSGELRSEEDLRLDVNEEGKLYLISFYEGLQRVALFTEDRRIYKLLCESEKAELAEQEIILSLQDVGVSLVNNSSRQEVSFIGITSSDVVWESKPKKKSRWKPLSGKDAELLETHFKEYTESGPVDNAILDLENNFQVSLTHNGQDMRMMQPYDAPIRRSFLPGVKVEYSVSARQSAYRVQINRIQIQNQLPGAIFPYVFYPIKLPKSVTMDSEPKPLTDISIVTRAAGHSDISRIKYFKVLIQEMDLKLDLGFLYAILDLFTPENASVMTSEQQVELFEKDIEYLKTELNDVSATDTSPISLYEYFHISPIKLHLSFSLSSGGEDGNKEKRETEIIPVQSLNLLLKSIGATLTDVQDVVFKLAFFELTFQFRTRQQLQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGIAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRAAETSQDVESLRPPRFIHEDGVIRPYKEREGIGSQMLQKIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKGTFGQLTCEWQYLFDEFTKEPMIVEGRRLRIEAKERVKSVFHAKEFGKIINFRSPEIAKWVLTKLEDARESLHKY

Target ORF information:

RefSeq Version	XM_021621041.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss vacuolar protein sorting 13 homolog A (vps13a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021621041.1

ORF Insert Sequence:

ATGGTCTTCG AAAGCGTGGT GGTAGATGTC CTAAACAGGT TTTTAGGGGA TTACGTTGTA 
AACCTTGACA GCTCACAACT CAAGCTCGGG ATTTGGGGAG GTGATGCAGT GCTCAGAAAT 
CTTGAAATAA AGGAAAATGC CTTGAGTCAA CTTGACATTC CTTTTAAAGT AAGAGCAGGG 
CATATAGGAC GCTTGGAGTT GAAGATTCCA TGGAAGAATC TGTACACCCA GTCAGTGGAA 
GCCACACTGG ATGGAGTCTA CCTACTCATC GTGCCCACAG CCAGTATAAA GTATGATGCA 
GAGAAGGAGG AGAAACAGCT CCAGGAGGCT CGTCAGAGGG AGCTGCAGAG GATCGAAGAG 
ACCAAACAGA AAGCAGCAGA GCAAGAAAAC CCTGCACTTG AGAAGCAGGA CACCTTCGTG 
GAGAAACTTG TGACGCAGGT GATCAAGAAC CTTCAAGTGA AGATCTCCAA CATCCACGTC 
CGCTATGAGG ATGATGTCAC CAACCCCAAT TGCCCCTTTT CATTTGGAGT GTCACTGCAG 
AACCTCAGCC TTCAGACAGC AGATGAGAAT TGGATGCCTT GTCTCCTGGA TGAGAAAGCC 
AAGCTGTTTT TCAAGCTTGT CCAGTTGGAC AATCTCTTTG CTTACTGGAA TGTGAACTCG 
ATGCTGTACT CCAATCACAT GCCAGATGAG GCCCTGAGAT GCCTCCAGGA CAGCATCCCG 
GGGGGTTCCA CTCCCATGAA CCACGATTTC ATTTTTCGTC CGATAACGGC CGATGCGAAG 
TTGCGCATGA ACCCCCGGTC AGATGTGGAC TTCTCCTCTC CCAAGGTGGA CCTGGTGGTC 
AACCTAAGAG AGGTGGCCGT GGAGCTCAAC AGACCACAGT ACATCAGCAT TCTGGAGCTC 
CTGGGATCAG TGGATATGAT GTCACGCAAC CTGCCCTACA GGAAATACCG GCCGCAAGTC 
CACGTGCACA CCAACGCCTA TCTATGGTGG CAGTATGTGA TCACAGGCAT CATGGAGGTG 
GACGTGAAGC CGCGGCTGCA CATGTGGTCG TGGCAGCACA TCCGCTGCCA CCGGCAGACA 
GTCAAGCAGT ACAGAGAGCT CTACAAGGCC AAGATCACCA GCAAGAAGCC CACTGAGAAG 
CAGCTCAGGG AGCTGGAGGA GCCTGAGCGG ACTCTGGATA TTTTTAACAT CACTCTGGCC 
AGGCAGCAAG CTGAGATGGA GGCAAGCAAA GCGGGGCTGC GTATCTTCCG TCCGGGGGTG 
AAGGTGGAGG AGGAGGAATC TCAGGGCTGG TTGGGCTGGA TGTGGTCCGG CTGGTCAGGA 
GACTGTGGCG CCGAGGCCAA GGAGGTCAAG ACCGGAGCTT TCGACGAGCT TATGACTTCT 
GCTGAGAAGG CCAAACTCTA CACCGCCATC GGCTACAGTG AGATAGCTGT CAATCATAAC 
CTGCCAAAGA ACTTTGAGGA TATGAAGATT AATTTCCACA TGGAGAGGAT GTCTGTCTCA 
ATGAAAGACA ACAAGGACCA GAATGAAATC CTCAAACTCA CAGTTGAAGA TCTGAAATCC 
ATGCTGACCC AGAGGCCCGG AGCACAAGCC ATTAAGCTGT CGGCCCAGCT CAGTCTGTTT 
GAGGTGACAG GACTAGCCAG TAACCGGCCG GCACCCACAT TGCTGTCGTC AAGGAAGGCT 
GCCACGGTAA CGGGGACGCC CTTGTTGGAC ATCCTGTTTG AGACCAACCC TCTGGATGAG 
AGCGCTGATC AGCGGCTCCG CGTCGAGTCC CAGCCTCTGG AGATCATCTA TGATGCTGTG 
ACGGTAAACA GCATGTCTGC GTTCTTCTCG CCGCCTGATG ACCTCCAGCT GGACGATCTG 
ACCAACGCCA CTCTCATGAA GCTGGAGCAG TTCCGAGACC GCACCTCCAC AGGACTGATG 
TACGTGATTG AGACCCAGAA GGTTCTGGAT CTGAACGTCA ACCTCATGGC CTCCTACGTA 
ATCGTTCCCC AAACCGGCTT CCACGACTCC ACCCAGAACC TCATGCTATT GGACCTGGGC 
CACTTCAAAG TGTCTAGTCT GAGCAGAGAG GGGCTGCCAC AGCTCTCTGT AGGGAGAAGT 
ACTATAGAGG ACATTATGTC CCGGGCCTAC GACAGCTTCG ACGTCCAGCT CAGCAGCCTT 
CAGTTCCTCT ACAGCAAACC AGATGGGGAC TGGAAGAAAG CCCGTAAGCT GAGGCAGTCG 
GCCCTCCACA TCCTGGAGCC TGTTGACGTC AAGGTGGTGT TCAGCAGAGC CATGGTGGTC 
ACAGATTCCC GCATGCCCAA GTTTAAAATC TCCGGGGAGC TGCCCCTCCT GTCTCTGAGG 
ATCTCTGATG ATAAGGTACG GGGCGTGCTG GAGCTGATCG ACAGCATCCC GCTGCCAGAG 
AGCAGGCCGG CCCCGCGCAG CACCAACGCT CACTCAACAT CCAAGGCTTT AAAGCAGTCG 
TACACCCCTC AGCTAACCCC CAGGAAACCA ACCATGGCTG AACTGCGCAA CTCCCTAATA 
TGGGAATCAG GCTCAGAGGA GGACCTGTTC TACGATGCTC CCAGCTCTCC TATAGGAGAC 
CGTCCGTTCT TCCCTGACAA CATGCCCAGC CCCTTGCAGC GCTCCGACTC AGTCAAGAGG 
AGAGGTCTGG TCAAGAAAGA CCCCAAGAAG AACATGACCG AGTTCCTCAT GACATTTGAG 
ATCAGCAAAC TGTCCGTTCA GCTGTGTCGT CTCTCTGGAG GCGAGGAGGT CACAGTGTTG 
CACCTGGACA TCGAGGGGCT GGGCACCGAG CTGAAGCTAC GTACCTTCGA CATGACATCC 
TACACGTTCC TACGAGAGAT CTGCCTGGAG TGCCCCGAGT ACATGGATTC AGAGGAGAAG 
AAAGTGCACC TGATCACCAC TCTAGACAAC ACAGAGGATG ACCTCCTCAC ATTGGAGTAC 
ACTAAGGCTG ATAAGAATGG GCCAGAATTC AAGTCTGAGT ACAAGAACAC TGAACAATCA 
GTTAAGGTGA ACTTCTCGTC TCTGGACATA CACCTGCACA CGGAGGCTCT GCTCAATGCC 
ATGAACTTCC TGATCAGTCT CCTCCCCCCG ACCAAGGAGG TGGCGCCCAG CCAGGAGGAG 
CTGCCCACCA TCCCCGAGGA GGATGAGGGA GAGGGGGAGG AAGGCGAGAA GAAAGAGGGA 
TCGGCTGTGG CAAAAAAAAC CTCTTCTAAG AAGTCAAAGT TTGAGGATGT GGTGAATTTG 
CACATCAGAG CCGATCTCAA CTGTCTGAAG GTTTTCATCC GAGGCCAGAG CCACAGGATA 
GCGGAGATCC GCATCGAGGG CCTGGTGTCG GAGGTGCTGA TGAGGAAAAA AGCGGTAGAG 
GTTCTGGCCA ACCTGAAGAA CATAGTCATC CTGGACTGTG ATAAGGAGGC GTTGTACAAG 
CAGTGTGTGT CCATAGCCGA TGAGGAGGTG TTTGCTTTCC GCATGGTGAA CCACACTGAA 
GCCACAGAGG GCGACGCCTA TCTAGACATG GGTAAAGTGG ACACCAGCGT CACCCTGACG 
GTGGGCTGCA TCCAGGTCAT CTTCCTCAAC AAGTTTGTCT CCTCCATCCT GGCCTTCATC 
AACAACTTCC AGGTGGCCAA GGAGGCCCTG GCTGAGGTGA CGGTGCAGGC AGCGGAGAAG 
GCGGCCACAG GGGTGATGGA GCTGGCTGAG CGCAGCACCC GCATCTCCCT GGACGTGGAC 
TTCAACGCTC CCGTCGTCTT CCTGCCGCAG TCGTCGTCCT CCGCCAACGT CATCGTGGCC 
GACCTCGGGA TGGTCACGGT CAAGAACCGC TTCGCCATGG TCCCCTCCCA AATGCACGTC 
AAGATCCCTC CCGTCATGGA CACCATGACC GTGGGCCTCA GCGACCTCAA GATGTACAGA 
ACCACCTTTG AGGAGGGGAG GTTTCAGGGG GAAATCCAGC TTCTGAAGCC GGTCAACCTG 
GACCTGTCCA TCCAGAGGAA CCTGTCAGCC TCCTGGTACC ACAGCATCCC CGACATAGAG 
ATCACTGCTC ACCTCAAACC AATGAACCTG ATCCTGGGTC AGGATGATAT GACGGTGGTG 
TTGAGGACTC TGAGTGGGAA TCTGTCAGAG ACGTCAGACT CTGCTTCCCC CCCTCCTCCT 
CCGCGTACTG TGGACCGCTC CGACACGGCC TCTACCAAAG GCTCACAGGC GTCAGGAGGC 
AACACGGTGG TAACTGCTGC AGTGGTGGAG ACCCAGAAGA GCACCAAGCT GAAGAGCACA 
CTCAAACTGG ACTTCAAGTT TGACTCCATG ACCCTGGTCC TGTACAGCCC CAATGGGACT 
GAGGTGCAGG TGTTGGACTC GAGGGATGAG CAGCTGAAGC TGGCTGAGTT TACCCTGGGG 
ACTATCTCTA CATCTGTCCA CATGTTCTCA GACGGATCTA TGAAGGCCTC CGTCAAACTC 
ACAGACTGCC TGCTAGACGA CAAAAGATGC AGGGTCAAGA AGATCACTGC CAGGATGATG 
GCGATGCGTC CGGGCGCTGA GAGGAACGTG ATGGTGGAGG TGAACTACAA GCAGGGCCGC 
GACGGCACCT CTTTGGACAC GGTGGTGCAG GACGTGTACC TCTGTGCCAG CATGGAGTTC 
CTGCTCACTG TGGCCGACAT CTTCCTCAAA GCCAGCCAGC AGGGCTTCTC CTCCGCCACC 
CCCAAGAACA GCAGTAGCAC TAAACCCACT GCAGCCGCAG CCTCCAAGGA ATCTGCTCCA 
GTGGGGGTGG TGGCAAAGTC AGAGATTAAT GTTCTGGTGA AGAACCCCGA GATTGTGTTT 
GTGGCCGACC TGACACGTGC CGACGCCCCG GCCCTGGTCA TGACCACGGA GTGTGAGCTG 
GTGATGAAGA GCGACCTGGA CGGTCAGCTG ATGACTGCGG TGGTCAAGGA CCTGAAGGTG 
GTGGCCTGCC CCTTCCTGAA GGAGAAGAGA AACAAGGAGA CCACTGTGCT GCAGCCCTGC 
CAAGTGTTCT TCAAGAGCAC ACAGTCCTCC ACCTCCCCGC AGGCCATGGA GGTCTGCATC 
AACGCACTCA CACTCAAGGT GTCGCCCATC ATTATCAACA CAGTGATTAC CATTCAGTCG 
GTGCTGAGCC CGGCCGGTGC CGAGACACCC CAGTCTGAGC TAGAGAGCCC CGTGCCCCCA 
GACCTGTGGG AGCGCCGGTC CTGGAGGGAC CTCAAGCTGT GGTTCCTGGA GGAGGAGGGT 
AAAGACGAGG ATGCCCAGTC CCTGGTCCCG CTGATTCCCC AGGGCGAGTC CCTGCAGTTG 
GCCATCAACT CCATCTGCCT GACACTGGAG GCAGGGGTGG GCCACCGCAC CGTCCCCATG 
CTCCTGGCCA AGTCCTCCTT CCACGGTGAC GTCAAGAACT GGAGCACCCT CATCAACCTG 
CACAGCCAGC TCAACCTGGA GGTTCACTAC TTCAACGAGG TGATGGGGGT GTGGGAGCCA 
CTGCTAGAGC CCCTAGAAGA CGAGACCCGA GATGGATTCA GACCCTGGAC CCTGGGATTA 
AAGATGAAGA AGAAGCCAGT GAAGTACACA GGTATTTCAG AAGAAGTGGA CTACAGCATC 
CCTGACTACA AGACTGTCAT CAGCATCAGC AGCAAGGACC AACTTAATAT CACCCTCTCC 
AAGTGTGGAC TGGGCATGCT CAGTAACCTG GGCACGGCCT TTGCGGAGGC GGCCAAGCAG 
GCATCGGATA GTTTCCAGAA GGACGAGGCT CCGTTCGTCG TGAAGAACCG TCTAGGTCTG 
ACTGTGTCGG TGGTGCACAG TGAGCTGTTC AGCCCCCTGG GTCGCGAGGC CACGGCGCGC 
CGGGTAGAGC TGCAGGACGG AGAGAGCCTC AACATGGACT ACCGTCGCAC CACCACCCAC 
TCAGACCAGT TCTCTGCCAT GACTTCATTG GTGGCCAAGG ACTACTACAT ACAGCCCACT 
CCGGTGGGCC ACACTTCAAC AGGCATGATC CCGTTGATCA AAGTGGGGCG TGGCATGTAC 
AGTGTCATGC ACAAAGACTC TGGGGTCACC CGTTTCCTGG TCTGTCAGAT CTACTCTGTG 
GAGGGAAGCA AGTACATCAA GATCCGCTCT CCCTTCCAGA TCATAAACCA CTTCTCCATC 
CCATTCAGAG TGATGGAGGG CTCTACCAGT TTGGGGATAT CACTGCCTAC AGAGGAGTTC 
TGTGTGCCTC TGGACTCCTA CAGGTCAGAG CTAAGACTGC AGCCGGTAAC CGAGGGAGAC 
GATCAGTACG ACTGCTCCGA GGGTTTCAGC TACGAGTTGG TGAGCCAGCA GCCCGGTGCA 
CGTGTGCAGC AGACCTGTCA TCGCCATGGT GACAGTGCTG GCGTCCTCAT GGTCAACATC 
GTCCCTCTGA AAGACACGGT GACATCACAA CACACAGGAA TGGCTGGGGA GCACTTTGAC 
GAGCCCTACA TCCTGCACCT GTGGCCCTCC GTCCTGCTTC GGAACCTTCT GCCTTACCCC 
ATCACCTATC GCCTACAGGA CAGTGAAGAG CCCACCCCCA TCACCCTGAT GGACGGCCAC 
TCGGCCCAGC TGCACACCGC CATCATGGAC CAGTCCAGCC TGGAACTCCG CCTCCTCGAC 
TACCTGGGTC AGGATTGGCA GGCCCACTAC GGTCTCTGTG GCGACCTGGA TGAGATCACC 
TTCATTGTGT TCAGCTCTCT GCGCGATGAA GAGGAGGTGG TCAGGTTAGG AGAGAGGGGA 
GAAGGGAAGA GGGCAGAGCT CGATATTGCG GTGCATGTGA AGTACGACGC GGGCCAGACT 
GAGGTGGCTA TCCATTCTCC CTACTGGATG GTGAACAAGA CGGGCCGCCT GCTGCAATAC 
AAGGCCGATG ACATCCACCG CAAACACGCC ATGGACTACG ATATGCCTCT GCTCTTCTCC 
TTCAAGCCAC ACAACTTTCT TCAGAACAAC AAGGTGCGGC TGATGATCTC GGACAGCGAC 
CTGTCGGATG ATTTCTCCCT GGATACCGTG GGTAGTTATG GTGAAGTGAA GTGCAAAGGG 
AGGCTGAAAG ATTACGTGAT CGGAGTGAAG ATCGACGCCA GCAGTTTCAG CCTGACCCGT 
ATCGTAAGCT TTGTGCCATA CTACCTGTTA GTGAACAGGA CCAAACATGT GATCTGGGTG 
GGAGAGGAGG GCCAAGACGA CTGGACCGAG GCCAGACCAC AACAGGCAGC CATCCCGTTT 
TGGCCTGAGA GGGACTCCAA GAAGCTCAAA GTCAAAGTAG AAGGCAGTCG GTCAACTCCT 
CAAACCATGG ACTTCACCAA ACCGGAAAAC TGCCTGCTGC TGCATCTGGA CAACACTCTG 
GGCGGGGTCA TTATGGATGT GAACCTATCG GACCACTCGA TTATCATCCG TTTCTCTGAC 
TACCACGACG GGGCCGCCCC CTTCCTCATC ATCAACCACA CCCAGGAGCA GACGGTGGAG 
TTCTACCAGA GTAGTCAGAA GGAGAAGGAC AAGCTAAGCC CCGGGAAGGC AATGTACTAC 
ACCTGGGCTG AGCCCACTGG ATCGCGCTCT CTGGCCTGGC AGTGTGGCAG CTACAGCGGC 
GAGCTGAGGA GCGAAGAGGA CCTGCGGTTG GACGTGAACG AGGAGGGTAA GCTGTACCTC 
ATCTCGTTCT ACGAGGGCCT CCAGCGGGTG GCGCTGTTCA CAGAGGACAG ACGCATATAC 
AAGCTGCTGT GTGAGAGTGA GAAGGCCGAG CTGGCCGAAC AAGAGATCAT CCTCTCCCTG 
CAAGACGTGG GCGTCTCCCT GGTCAACAAC AGCAGCCGCC AGGAGGTGTC CTTCATCGGT 
ATTACCAGTT CAGACGTGGT GTGGGAGTCC AAGCCAAAGA AGAAGTCTCG CTGGAAACCC 
CTGAGTGGAA AAGATGCCGA GCTGCTGGAG ACCCACTTCA AAGAGTACAC AGAGAGTGGA 
CCAGTGGACA ACGCCATCTT AGACCTGGAG AACAACTTCC AGGTGTCTTT GACCCATAAT 
GGGCAAGACA TGAGGATGAT GCAGCCATAT GACGCTCCAA TCAGGAGGAG CTTCCTGCCA 
GGGGTGAAGG TGGAGTACAG CGTATCGGCC CGCCAGAGTG CCTACCGTGT CCAGATCAAC 
CGCATACAGA TCCAGAATCA GCTACCTGGA GCAATCTTCC CATATGTCTT CTACCCTATA 
AAACTACCTA AGTCTGTCAC CATGGACTCA GAGCCCAAAC CTCTGACCGA CATCAGCATT 
GTTACCAGGG CAGCAGGGCA CTCTGATATC TCACGCATCA AGTATTTCAA GGTGCTAATC 
CAGGAGATGG ATCTGAAGTT GGACTTGGGC TTCCTTTATG CCATCTTGGA TCTGTTCACT 
CCGGAGAACG CCAGCGTGAT GACGTCAGAG CAGCAGGTGG AGTTGTTTGA GAAGGACATA 
GAGTATCTGA AGACGGAGCT CAACGACGTC TCGGCCACTG ACACATCCCC TATCAGTCTC 
TATGAGTACT TCCACATCTC TCCTATCAAG CTGCACCTCA GTTTCTCTCT GAGTTCTGGA 
GGAGAGGATG GTAACAAGGA GAAGAGGGAG ACCGAGATCA TCCCTGTCCA GTCCCTCAAT 
CTGCTGCTGA AAAGCATCGG AGCAACCCTC ACTGACGTGC AGGATGTCGT CTTCAAATTG 
GCCTTCTTCG AGCTGACCTT CCAGTTTCGC ACTCGCCAGC AACTACAATG GGAGGTCATC 
AGACACTACT CGAAACAGGC GATAAAGCAG ATGTATGTGC TGGTGTTGGG CTTGGACGTG 
CTGGGAAACC CCTTTGGACT GATCCGAGGT CTGTCGGAGG GAGTGGAGGC TTTCTTCTAC 
GAGCCCTATC AGGGAGCTAT CCAGGGGCCA GAGGAGTTTG TGGAGGGTAT GGCGCTGGGG 
GTGAAAGCGC TGGTTGGAGG AGCAGTAGGT GGCATTGCGG GCGCTGCCTC CAGGATCACA 
GGCGCCATGG CGAAGGGTGT GGCTGCCATG ACAATGGACG AGGAGTACCA GCAGAAGAGA 
CGGGAGGCTA TGAACAAGCA GCCTAGCGGC CTGAGAGAGG GCCTGACCCG CGGAGGCAAA 
GGACTGGTGT CTGGCTTTGT CAGTGGGATC ACAGGGATTG TCACCAAGCC CATTAAAGGT 
GCTCAGAAGG AGGGCGCGGC GGGCTTCTTT AAGGGTGTGG GCAAGGGGCT AGTGGGTGCT 
GTGGCCAGAC CCACTGGAGG CATCATCGAC ATGGCCAGCA GCACCTTCCA GGGCATCAAG 
AGGGCAGCTG AGACATCCCA GGATGTGGAG AGTCTGCGTC CGCCTCGCTT CATCCATGAG 
GACGGGGTCA TCCGACCATA CAAGGAAAGA GAGGGCATCG GCAGCCAGAT GCTACAGAAA 
ATTGAAAACG GAAGGTTCGC CAAGTACAGA TACTTTGCGC ACGCCAAAGT GAACGAGTCC 
GATTTCCTGA TGATCACCAA AAGGGGAATC TTTTTCGTGA CCAAAGGTAC ATTTGGGCAG 
CTGACCTGCG AGTGGCAGTA TCTGTTCGAC GAGTTCACCA AGGAGCCCAT GATCGTGGAG 
GGGAGGCGGC TTCGGATCGA GGCCAAGGAG AGAGTGAAGT CTGTGTTTCA TGCCAAAGAG 
TTTGGGAAGA TAATCAACTT CAGAAGTCCA GAAATAGCCA AGTGGGTTCT TACCAAGCTT 
GAAGACGCCA GGGAGAGTCT ACACAAATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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vps13a cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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