ezh2 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	ezh2
Entrez Gene ID	110535410
Full Name	enhancer of zeste 2 polycomb repressive complex 2 subunit
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021620394.1	XP_021476069.1	histone-lysine N-methyltransferase EZH2 isoform X1
XM_021620396.1	XP_021476071.1	histone-lysine N-methyltransferase EZH2 isoform X3
XM_021620397.1	XP_021476072.1	histone-lysine N-methyltransferase EZH2 isoform X4
XM_021620395.1	XP_021476070.1	histone-lysine N-methyltransferase EZH2 isoform X2

Pathways from BioSystems
Gene Ontology

The following ezh2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ezh2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOf56006	XM_021620394.1 Latest version!	Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OOf56007	XM_021620395.1 Latest version!	Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OOf56008	XM_021620396.1 Latest version!	Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OOf56009	XM_021620397.1 Latest version!	Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ezh2 ( XM_021620394.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021476069.1 Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf56006
Clone ID Related Accession (Same CDS sequence)	XM_021620394.1
Accession Version	XM_021620394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	histone-lysine N-methyltransferase EZH2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAACA CACTGCTACA GCAGAACTTC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAAACA CACTGCTACA GCAGAACTCC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAAACA CACTGTTACA GCAGAACTTC ATGGTAGAAG ACGAAACGGT TCTCCACAAC 
ATCCCTTACA TGGGAGACGA GATTCTGGAC CAAGACGGAA CCTTCATAGA AGAACTCATC 
AAGAACTACG ATGGCAAAGT TCACGGAGAC AGGGAGTGCG GCTTCATCAA CGATGAGATC 
TTTGTGGAGC TGGTGGGTGC CCTGACCCAG TACAGTGACA ACGATGATGA GGATGATGAT 
GAAGAAGAGC AGGAGTTTAA GCTTGAGAAG ATGGAGCTCT GTGAGGCGAA GGACCACCTG 
GCCGAGGACC CCCGAAAGGA ACCCCTGATC AACACTGACA GCCAAGGCAG CAGTGACAGC 
TCTAAGAAGT TTCCGTCTGA TAAGATCTTT GAAGCCATCT CCTCAATGTT CCCTGATAAG 
GGCTCAACAG AGGAACTTCG AGAAAAGTAC AAGGAGCTGA CAGAGCCCCA GTTGCCTGGT 
GCGTTGCCTC CAGAGTGTAC TCCTAACATG GACGGTCCCA ACGCTCGCTC CGTTCAGAGG 
GAACAGAGTC TACACTCCTT CCACACACTC TTCTGCCGAC GCTGCTTCAA ATACGACTGC 
TTCCTGCACC GTGAGTCCTT CCATGCGACT CCTAACACCT ATAAGCGTAA GAACATGGAG 
AATCTGGTAG ACAGTAAACC CTGTGGTGAC GAGTGCTACA TGTACCTGGT GAAGGATGGA 
TTGGTGAGGG AGTATCCTGA CGGCATGGCA ACCGAGAGGT CCAAGACCCC TTCCAAACGC 
CCAGTGAGCC GTCGCCGGGG ACGACCCATC GGCAACAGCC GGCCTAGTAC ACCAACAGTC 
TCCACCGACA CCAAAGACAC GGACAGCGAG CAGGAGGGGA AAGACGACGA CGATAATGAT 
GATGATGATG ATGATGATGA TGACAAGAAG GACGAGACCA CCAGCAGTTC AGAGGGTAAC 
TCACGGTGCC AGACTCCAGT GAAGTTGAAG CTGACGTGTG ACCCTCAGGT TGTTGATTGG 
AGCGGAGCCG AGGCTTCACT CTTTAGGGTT CTCATCGGAA CCTACTACGA CAACTACTGC 
GCTATAGCAC GGCTCATAGG AACCAAGACC TGCAGACAGG TATATGAGTT CAGAGTGAAG 
GAGTCTAGTA TCATCGCTCG CGCTCCGGCC GAGGACGAAG ACACGCCCCC TCGCAAGAAG 
AAGAGGAAAC ACAGGTTGTG GGCCACTCAC TGCAGGAAGA TCCAACTGAA GAAAGATGGT 
TCGTCCAACC ACGTGTATAA CTACCAGCCA TGTGACCACC CCCGCCAGCC CTGTGACTCC 
TCCTGTCCCT GTGTCACTGC TCAGAACTTC TGTGAGAAGT TCTGCCAGTG CAGCTCAGAG 
TGTCAGAACC GTTTCCCAGG CTGCAGGTGT AAGGCCCAGT GTAACACTAA ACAGTGTCCG 
TGTTACCTGG CGGTCAGAGA GTGTGACCCT GACCTGTGTC TGACCTGCGG AGCCGCTGAA 
CACTGGGACA GCAAGAACGT CTCCTGTAAA AACTGCTCCA TACAGAGGGG AGCCAAGAAG 
CACCTGCTCC TAGCTCCGTC TGATGTAGCA GGCTGGGGCA TCTTCATCAA AGAGCCAGTT 
CAGAAGAATG AGTTCATCTC TGAGTACTGT GGGGAGATAA TCTCCCAAGA TGAGGCGGAT 
CGCAGAGGGA AGGTCTACGA CAAATACATG TGTAGCTTCC TCTTCAACCT CAACAACGAT 
TTTGTAGTGG ATGCTACTAG GAAAGGAAAC AAGATTCGTT TCGCCAACCA TTCCGTCCAC 
CCCAACTGCT ATGCAAAAGT GATGATGGTG AATGGGGACC ACAGGATAGG GATCTTTGCT 
AAGAGAACCA TCCAGACTGG AGAAGAGCTC TTCTTTGACT ACCGGTACAG TCAAGCAGAT 
GCTCTGAAGT ACGTCGGCAT CGAGCGGGAA TCGGAGATGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021476069.1
CDS	22..2106
Translation	MVNTLLQQNFMVNTLLQQNFMVNTLLQQNSMVNTLLQQNFMVNTLLQQNFMVEDETVLHNIPYMGDEILDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVGALTQYSDNDDEDDDEEEQEFKLEKMELCEAKDHLAEDPRKEPLINTDSQGSSDSSKKFPSDKIFEAISSMFPDKGSTEELREKYKELTEPQLPGALPPECTPNMDGPNARSVQREQSLHSFHTLFCRRCFKYDCFLHRESFHATPNTYKRKNMENLVDSKPCGDECYMYLVKDGLVREYPDGMATERSKTPSKRPVSRRRGRPIGNSRPSTPTVSTDTKDTDSEQEGKDDDDNDDDDDDDDDKKDETTSSSEGNSRCQTPVKLKLTCDPQVVDWSGAEASLFRVLIGTYYDNYCAIARLIGTKTCRQVYEFRVKESSIIARAPAEDEDTPPRKKKRKHRLWATHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVTAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAAEHWDSKNVSCKNCSIQRGAKKHLLLAPSDVAGWGIFIKEPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVHPNCYAKVMMVNGDHRIGIFAKRTIQTGEELFFDYRYSQADALKYVGIERESEMP

Target ORF information:

RefSeq Version	XM_021620394.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021620394.1

ORF Insert Sequence:

ATGGTAAACA CACTGCTACA GCAGAACTTC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAAACA CACTGCTACA GCAGAACTCC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAAACA CACTGTTACA GCAGAACTTC ATGGTAGAAG ACGAAACGGT TCTCCACAAC 
ATCCCTTACA TGGGAGACGA GATTCTGGAC CAAGACGGAA CCTTCATAGA AGAACTCATC 
AAGAACTACG ATGGCAAAGT TCACGGAGAC AGGGAGTGCG GCTTCATCAA CGATGAGATC 
TTTGTGGAGC TGGTGGGTGC CCTGACCCAG TACAGTGACA ACGATGATGA GGATGATGAT 
GAAGAAGAGC AGGAGTTTAA GCTTGAGAAG ATGGAGCTCT GTGAGGCGAA GGACCACCTG 
GCCGAGGACC CCCGAAAGGA ACCCCTGATC AACACTGACA GCCAAGGCAG CAGTGACAGC 
TCTAAGAAGT TTCCGTCTGA TAAGATCTTT GAAGCCATCT CCTCAATGTT CCCTGATAAG 
GGCTCAACAG AGGAACTTCG AGAAAAGTAC AAGGAGCTGA CAGAGCCCCA GTTGCCTGGT 
GCGTTGCCTC CAGAGTGTAC TCCTAACATG GACGGTCCCA ACGCTCGCTC CGTTCAGAGG 
GAACAGAGTC TACACTCCTT CCACACACTC TTCTGCCGAC GCTGCTTCAA ATACGACTGC 
TTCCTGCACC GTGAGTCCTT CCATGCGACT CCTAACACCT ATAAGCGTAA GAACATGGAG 
AATCTGGTAG ACAGTAAACC CTGTGGTGAC GAGTGCTACA TGTACCTGGT GAAGGATGGA 
TTGGTGAGGG AGTATCCTGA CGGCATGGCA ACCGAGAGGT CCAAGACCCC TTCCAAACGC 
CCAGTGAGCC GTCGCCGGGG ACGACCCATC GGCAACAGCC GGCCTAGTAC ACCAACAGTC 
TCCACCGACA CCAAAGACAC GGACAGCGAG CAGGAGGGGA AAGACGACGA CGATAATGAT 
GATGATGATG ATGATGATGA TGACAAGAAG GACGAGACCA CCAGCAGTTC AGAGGGTAAC 
TCACGGTGCC AGACTCCAGT GAAGTTGAAG CTGACGTGTG ACCCTCAGGT TGTTGATTGG 
AGCGGAGCCG AGGCTTCACT CTTTAGGGTT CTCATCGGAA CCTACTACGA CAACTACTGC 
GCTATAGCAC GGCTCATAGG AACCAAGACC TGCAGACAGG TATATGAGTT CAGAGTGAAG 
GAGTCTAGTA TCATCGCTCG CGCTCCGGCC GAGGACGAAG ACACGCCCCC TCGCAAGAAG 
AAGAGGAAAC ACAGGTTGTG GGCCACTCAC TGCAGGAAGA TCCAACTGAA GAAAGATGGT 
TCGTCCAACC ACGTGTATAA CTACCAGCCA TGTGACCACC CCCGCCAGCC CTGTGACTCC 
TCCTGTCCCT GTGTCACTGC TCAGAACTTC TGTGAGAAGT TCTGCCAGTG CAGCTCAGAG 
TGTCAGAACC GTTTCCCAGG CTGCAGGTGT AAGGCCCAGT GTAACACTAA ACAGTGTCCG 
TGTTACCTGG CGGTCAGAGA GTGTGACCCT GACCTGTGTC TGACCTGCGG AGCCGCTGAA 
CACTGGGACA GCAAGAACGT CTCCTGTAAA AACTGCTCCA TACAGAGGGG AGCCAAGAAG 
CACCTGCTCC TAGCTCCGTC TGATGTAGCA GGCTGGGGCA TCTTCATCAA AGAGCCAGTT 
CAGAAGAATG AGTTCATCTC TGAGTACTGT GGGGAGATAA TCTCCCAAGA TGAGGCGGAT 
CGCAGAGGGA AGGTCTACGA CAAATACATG TGTAGCTTCC TCTTCAACCT CAACAACGAT 
TTTGTAGTGG ATGCTACTAG GAAAGGAAAC AAGATTCGTT TCGCCAACCA TTCCGTCCAC 
CCCAACTGCT ATGCAAAAGT GATGATGGTG AATGGGGACC ACAGGATAGG GATCTTTGCT 
AAGAGAACCA TCCAGACTGG AGAAGAGCTC TTCTTTGACT ACCGGTACAG TCAAGCAGAT 
GCTCTGAAGT ACGTCGGCAT CGAGCGGGAA TCGGAGATGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ezh2 ( XM_021620395.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021476070.1 Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf56007
Clone ID Related Accession (Same CDS sequence)	XM_021620395.1
Accession Version	XM_021620395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	histone-lysine N-methyltransferase EZH2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAACA CACTGCTACA GCAGAACTTC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAAACA CACTGCTACA GCAGAACTCC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAAACA CACTGTTACA GCAGAACTTC ATGGTAGAAG ACGAAACGGT TCTCCACAAC 
ATCCCTTACA TGGGAGACGA GATTCTGGAC CAAGACGGAA CCTTCATAGA AGAACTCATC 
AAGAACTACG ATGGCAAAGT TCACGGAGAC AGGGAGTGCG GCTTCATCAA CGATGAGATC 
TTTGTGGAGC TGGTGGGTGC CCTGACCCAG TACAGTGACA ACGATGATGA GGATGATGAT 
GAAGAAGAGC AGGAGTTTAA GCTTGAGAAG ATGGAGCTCT GTGAGGCGAA GGACCACCTG 
GCCGAGGACC CCCGAAAGGA ACCCCTGATC AACACTGACA GCCAAGGCAG CAGTGACAGC 
TCTAAGAAGT TTCCGTCTGA TAAGATCTTT GAAGCCATCT CCTCAATGTT CCCTGATAAG 
GGCTCAACAG AGGAACTTCG AGAAAAGTAC AAGGAGCTGA CAGAGCCCCA GTTGCCTGGT 
GCGTTGCCTC CAGAGTGTAC TCCTAACATG GACGGTCCCA ACGCTCGCTC CGTTCAGAGG 
GAACAGAGTC TACACTCCTT CCACACACTC TTCTGCCGAC GCTGCTTCAA ATACGACTGC 
TTCCTGCACC CCTTCCATGC GACTCCTAAC ACCTATAAGC GTAAGAACAT GGAGAATCTG 
GTAGACAGTA AACCCTGTGG TGACGAGTGC TACATGTACC TGGTGAAGGA TGGATTGGTG 
AGGGAGTATC CTGACGGCAT GGCAACCGAG AGGTCCAAGA CCCCTTCCAA ACGCCCAGTG 
AGCCGTCGCC GGGGACGACC CATCGGCAAC AGCCGGCCTA GTACACCAAC AGTCTCCACC 
GACACCAAAG ACACGGACAG CGAGCAGGAG GGGAAAGACG ACGACGATAA TGATGATGAT 
GATGATGATG ATGATGACAA GAAGGACGAG ACCACCAGCA GTTCAGAGGG TAACTCACGG 
TGCCAGACTC CAGTGAAGTT GAAGCTGACG TGTGACCCTC AGGTTGTTGA TTGGAGCGGA 
GCCGAGGCTT CACTCTTTAG GGTTCTCATC GGAACCTACT ACGACAACTA CTGCGCTATA 
GCACGGCTCA TAGGAACCAA GACCTGCAGA CAGGTATATG AGTTCAGAGT GAAGGAGTCT 
AGTATCATCG CTCGCGCTCC GGCCGAGGAC GAAGACACGC CCCCTCGCAA GAAGAAGAGG 
AAACACAGGT TGTGGGCCAC TCACTGCAGG AAGATCCAAC TGAAGAAAGA TGGTTCGTCC 
AACCACGTGT ATAACTACCA GCCATGTGAC CACCCCCGCC AGCCCTGTGA CTCCTCCTGT 
CCCTGTGTCA CTGCTCAGAA CTTCTGTGAG AAGTTCTGCC AGTGCAGCTC AGAGTGTCAG 
AACCGTTTCC CAGGCTGCAG GTGTAAGGCC CAGTGTAACA CTAAACAGTG TCCGTGTTAC 
CTGGCGGTCA GAGAGTGTGA CCCTGACCTG TGTCTGACCT GCGGAGCCGC TGAACACTGG 
GACAGCAAGA ACGTCTCCTG TAAAAACTGC TCCATACAGA GGGGAGCCAA GAAGCACCTG 
CTCCTAGCTC CGTCTGATGT AGCAGGCTGG GGCATCTTCA TCAAAGAGCC AGTTCAGAAG 
AATGAGTTCA TCTCTGAGTA CTGTGGGGAG ATAATCTCCC AAGATGAGGC GGATCGCAGA 
GGGAAGGTCT ACGACAAATA CATGTGTAGC TTCCTCTTCA ACCTCAACAA CGATTTTGTA 
GTGGATGCTA CTAGGAAAGG AAACAAGATT CGTTTCGCCA ACCATTCCGT CCACCCCAAC 
TGCTATGCAA AAGTGATGAT GGTGAATGGG GACCACAGGA TAGGGATCTT TGCTAAGAGA 
ACCATCCAGA CTGGAGAAGA GCTCTTCTTT GACTACCGGT ACAGTCAAGC AGATGCTCTG 
AAGTACGTCG GCATCGAGCG GGAATCGGAG ATGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021476070.1
CDS	22..2100
Translation	MVNTLLQQNFMVNTLLQQNFMVNTLLQQNSMVNTLLQQNFMVNTLLQQNFMVEDETVLHNIPYMGDEILDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVGALTQYSDNDDEDDDEEEQEFKLEKMELCEAKDHLAEDPRKEPLINTDSQGSSDSSKKFPSDKIFEAISSMFPDKGSTEELREKYKELTEPQLPGALPPECTPNMDGPNARSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNMENLVDSKPCGDECYMYLVKDGLVREYPDGMATERSKTPSKRPVSRRRGRPIGNSRPSTPTVSTDTKDTDSEQEGKDDDDNDDDDDDDDDKKDETTSSSEGNSRCQTPVKLKLTCDPQVVDWSGAEASLFRVLIGTYYDNYCAIARLIGTKTCRQVYEFRVKESSIIARAPAEDEDTPPRKKKRKHRLWATHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVTAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAAEHWDSKNVSCKNCSIQRGAKKHLLLAPSDVAGWGIFIKEPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVHPNCYAKVMMVNGDHRIGIFAKRTIQTGEELFFDYRYSQADALKYVGIERESEMP

Target ORF information:

RefSeq Version	XM_021620395.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021620395.1

ORF Insert Sequence:

ATGGTAAACA CACTGCTACA GCAGAACTTC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAAACA CACTGCTACA GCAGAACTCC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAAACA CACTGTTACA GCAGAACTTC ATGGTAGAAG ACGAAACGGT TCTCCACAAC 
ATCCCTTACA TGGGAGACGA GATTCTGGAC CAAGACGGAA CCTTCATAGA AGAACTCATC 
AAGAACTACG ATGGCAAAGT TCACGGAGAC AGGGAGTGCG GCTTCATCAA CGATGAGATC 
TTTGTGGAGC TGGTGGGTGC CCTGACCCAG TACAGTGACA ACGATGATGA GGATGATGAT 
GAAGAAGAGC AGGAGTTTAA GCTTGAGAAG ATGGAGCTCT GTGAGGCGAA GGACCACCTG 
GCCGAGGACC CCCGAAAGGA ACCCCTGATC AACACTGACA GCCAAGGCAG CAGTGACAGC 
TCTAAGAAGT TTCCGTCTGA TAAGATCTTT GAAGCCATCT CCTCAATGTT CCCTGATAAG 
GGCTCAACAG AGGAACTTCG AGAAAAGTAC AAGGAGCTGA CAGAGCCCCA GTTGCCTGGT 
GCGTTGCCTC CAGAGTGTAC TCCTAACATG GACGGTCCCA ACGCTCGCTC CGTTCAGAGG 
GAACAGAGTC TACACTCCTT CCACACACTC TTCTGCCGAC GCTGCTTCAA ATACGACTGC 
TTCCTGCACC CCTTCCATGC GACTCCTAAC ACCTATAAGC GTAAGAACAT GGAGAATCTG 
GTAGACAGTA AACCCTGTGG TGACGAGTGC TACATGTACC TGGTGAAGGA TGGATTGGTG 
AGGGAGTATC CTGACGGCAT GGCAACCGAG AGGTCCAAGA CCCCTTCCAA ACGCCCAGTG 
AGCCGTCGCC GGGGACGACC CATCGGCAAC AGCCGGCCTA GTACACCAAC AGTCTCCACC 
GACACCAAAG ACACGGACAG CGAGCAGGAG GGGAAAGACG ACGACGATAA TGATGATGAT 
GATGATGATG ATGATGACAA GAAGGACGAG ACCACCAGCA GTTCAGAGGG TAACTCACGG 
TGCCAGACTC CAGTGAAGTT GAAGCTGACG TGTGACCCTC AGGTTGTTGA TTGGAGCGGA 
GCCGAGGCTT CACTCTTTAG GGTTCTCATC GGAACCTACT ACGACAACTA CTGCGCTATA 
GCACGGCTCA TAGGAACCAA GACCTGCAGA CAGGTATATG AGTTCAGAGT GAAGGAGTCT 
AGTATCATCG CTCGCGCTCC GGCCGAGGAC GAAGACACGC CCCCTCGCAA GAAGAAGAGG 
AAACACAGGT TGTGGGCCAC TCACTGCAGG AAGATCCAAC TGAAGAAAGA TGGTTCGTCC 
AACCACGTGT ATAACTACCA GCCATGTGAC CACCCCCGCC AGCCCTGTGA CTCCTCCTGT 
CCCTGTGTCA CTGCTCAGAA CTTCTGTGAG AAGTTCTGCC AGTGCAGCTC AGAGTGTCAG 
AACCGTTTCC CAGGCTGCAG GTGTAAGGCC CAGTGTAACA CTAAACAGTG TCCGTGTTAC 
CTGGCGGTCA GAGAGTGTGA CCCTGACCTG TGTCTGACCT GCGGAGCCGC TGAACACTGG 
GACAGCAAGA ACGTCTCCTG TAAAAACTGC TCCATACAGA GGGGAGCCAA GAAGCACCTG 
CTCCTAGCTC CGTCTGATGT AGCAGGCTGG GGCATCTTCA TCAAAGAGCC AGTTCAGAAG 
AATGAGTTCA TCTCTGAGTA CTGTGGGGAG ATAATCTCCC AAGATGAGGC GGATCGCAGA 
GGGAAGGTCT ACGACAAATA CATGTGTAGC TTCCTCTTCA ACCTCAACAA CGATTTTGTA 
GTGGATGCTA CTAGGAAAGG AAACAAGATT CGTTTCGCCA ACCATTCCGT CCACCCCAAC 
TGCTATGCAA AAGTGATGAT GGTGAATGGG GACCACAGGA TAGGGATCTT TGCTAAGAGA 
ACCATCCAGA CTGGAGAAGA GCTCTTCTTT GACTACCGGT ACAGTCAAGC AGATGCTCTG 
AAGTACGTCG GCATCGAGCG GGAATCGGAG ATGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ezh2 ( XM_021620396.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021476071.1 Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOf56008
Clone ID Related Accession (Same CDS sequence)	XM_021620396.1
Accession Version	XM_021620396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	histone-lysine N-methyltransferase EZH2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAACA CACTGCTACA GCAGAACTTC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAAACA CACTGCTACA GCAGAACTCC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAGAAG ACGAAACGGT TCTCCACAAC ATCCCTTACA TGGGAGACGA GATTCTGGAC 
CAAGACGGAA CCTTCATAGA AGAACTCATC AAGAACTACG ATGGCAAAGT TCACGGAGAC 
AGGGAGTGCG GCTTCATCAA CGATGAGATC TTTGTGGAGC TGGTGGGTGC CCTGACCCAG 
TACAGTGACA ACGATGATGA GGATGATGAT GAAGAAGAGC AGGAGTTTAA GCTTGAGAAG 
ATGGAGCTCT GTGAGGCGAA GGACCACCTG GCCGAGGACC CCCGAAAGGA ACCCCTGATC 
AACACTGACA GCCAAGGCAG CAGTGACAGC TCTAAGAAGT TTCCGTCTGA TAAGATCTTT 
GAAGCCATCT CCTCAATGTT CCCTGATAAG GGCTCAACAG AGGAACTTCG AGAAAAGTAC 
AAGGAGCTGA CAGAGCCCCA GTTGCCTGGT GCGTTGCCTC CAGAGTGTAC TCCTAACATG 
GACGGTCCCA ACGCTCGCTC CGTTCAGAGG GAACAGAGTC TACACTCCTT CCACACACTC 
TTCTGCCGAC GCTGCTTCAA ATACGACTGC TTCCTGCACC GTGAGTCCTT CCATGCGACT 
CCTAACACCT ATAAGCGTAA GAACATGGAG AATCTGGTAG ACAGTAAACC CTGTGGTGAC 
GAGTGCTACA TGTACCTGGT GAAGGATGGA TTGGTGAGGG AGTATCCTGA CGGCATGGCA 
ACCGAGAGGT CCAAGACCCC TTCCAAACGC CCAGTGAGCC GTCGCCGGGG ACGACCCATC 
GGCAACAGCC GGCCTAGTAC ACCAACAGTC TCCACCGACA CCAAAGACAC GGACAGCGAG 
CAGGAGGGGA AAGACGACGA CGATAATGAT GATGATGATG ATGATGATGA TGACAAGAAG 
GACGAGACCA CCAGCAGTTC AGAGGGTAAC TCACGGTGCC AGACTCCAGT GAAGTTGAAG 
CTGACGTGTG ACCCTCAGGT TGTTGATTGG AGCGGAGCCG AGGCTTCACT CTTTAGGGTT 
CTCATCGGAA CCTACTACGA CAACTACTGC GCTATAGCAC GGCTCATAGG AACCAAGACC 
TGCAGACAGG TATATGAGTT CAGAGTGAAG GAGTCTAGTA TCATCGCTCG CGCTCCGGCC 
GAGGACGAAG ACACGCCCCC TCGCAAGAAG AAGAGGAAAC ACAGGTTGTG GGCCACTCAC 
TGCAGGAAGA TCCAACTGAA GAAAGATGGT TCGTCCAACC ACGTGTATAA CTACCAGCCA 
TGTGACCACC CCCGCCAGCC CTGTGACTCC TCCTGTCCCT GTGTCACTGC TCAGAACTTC 
TGTGAGAAGT TCTGCCAGTG CAGCTCAGAG TGTCAGAACC GTTTCCCAGG CTGCAGGTGT 
AAGGCCCAGT GTAACACTAA ACAGTGTCCG TGTTACCTGG CGGTCAGAGA GTGTGACCCT 
GACCTGTGTC TGACCTGCGG AGCCGCTGAA CACTGGGACA GCAAGAACGT CTCCTGTAAA 
AACTGCTCCA TACAGAGGGG AGCCAAGAAG CACCTGCTCC TAGCTCCGTC TGATGTAGCA 
GGCTGGGGCA TCTTCATCAA AGAGCCAGTT CAGAAGAATG AGTTCATCTC TGAGTACTGT 
GGGGAGATAA TCTCCCAAGA TGAGGCGGAT CGCAGAGGGA AGGTCTACGA CAAATACATG 
TGTAGCTTCC TCTTCAACCT CAACAACGAT TTTGTAGTGG ATGCTACTAG GAAAGGAAAC 
AAGATTCGTT TCGCCAACCA TTCCGTCCAC CCCAACTGCT ATGCAAAAGT GATGATGGTG 
AATGGGGACC ACAGGATAGG GATCTTTGCT AAGAGAACCA TCCAGACTGG AGAAGAGCTC 
TTCTTTGACT ACCGGTACAG TCAAGCAGAT GCTCTGAAGT ACGTCGGCAT CGAGCGGGAA 
TCGGAGATGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021476071.1
CDS	22..2076
Translation	MVNTLLQQNFMVNTLLQQNFMVNTLLQQNSMVNTLLQQNFMVEDETVLHNIPYMGDEILDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVGALTQYSDNDDEDDDEEEQEFKLEKMELCEAKDHLAEDPRKEPLINTDSQGSSDSSKKFPSDKIFEAISSMFPDKGSTEELREKYKELTEPQLPGALPPECTPNMDGPNARSVQREQSLHSFHTLFCRRCFKYDCFLHRESFHATPNTYKRKNMENLVDSKPCGDECYMYLVKDGLVREYPDGMATERSKTPSKRPVSRRRGRPIGNSRPSTPTVSTDTKDTDSEQEGKDDDDNDDDDDDDDDKKDETTSSSEGNSRCQTPVKLKLTCDPQVVDWSGAEASLFRVLIGTYYDNYCAIARLIGTKTCRQVYEFRVKESSIIARAPAEDEDTPPRKKKRKHRLWATHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVTAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAAEHWDSKNVSCKNCSIQRGAKKHLLLAPSDVAGWGIFIKEPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVHPNCYAKVMMVNGDHRIGIFAKRTIQTGEELFFDYRYSQADALKYVGIERESEMP

Target ORF information:

RefSeq Version	XM_021620396.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021620396.1

ORF Insert Sequence:

ATGGTAAACA CACTGCTACA GCAGAACTTC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAAACA CACTGCTACA GCAGAACTCC ATGGTAAACA CACTGCTACA GCAGAACTTC 
ATGGTAGAAG ACGAAACGGT TCTCCACAAC ATCCCTTACA TGGGAGACGA GATTCTGGAC 
CAAGACGGAA CCTTCATAGA AGAACTCATC AAGAACTACG ATGGCAAAGT TCACGGAGAC 
AGGGAGTGCG GCTTCATCAA CGATGAGATC TTTGTGGAGC TGGTGGGTGC CCTGACCCAG 
TACAGTGACA ACGATGATGA GGATGATGAT GAAGAAGAGC AGGAGTTTAA GCTTGAGAAG 
ATGGAGCTCT GTGAGGCGAA GGACCACCTG GCCGAGGACC CCCGAAAGGA ACCCCTGATC 
AACACTGACA GCCAAGGCAG CAGTGACAGC TCTAAGAAGT TTCCGTCTGA TAAGATCTTT 
GAAGCCATCT CCTCAATGTT CCCTGATAAG GGCTCAACAG AGGAACTTCG AGAAAAGTAC 
AAGGAGCTGA CAGAGCCCCA GTTGCCTGGT GCGTTGCCTC CAGAGTGTAC TCCTAACATG 
GACGGTCCCA ACGCTCGCTC CGTTCAGAGG GAACAGAGTC TACACTCCTT CCACACACTC 
TTCTGCCGAC GCTGCTTCAA ATACGACTGC TTCCTGCACC GTGAGTCCTT CCATGCGACT 
CCTAACACCT ATAAGCGTAA GAACATGGAG AATCTGGTAG ACAGTAAACC CTGTGGTGAC 
GAGTGCTACA TGTACCTGGT GAAGGATGGA TTGGTGAGGG AGTATCCTGA CGGCATGGCA 
ACCGAGAGGT CCAAGACCCC TTCCAAACGC CCAGTGAGCC GTCGCCGGGG ACGACCCATC 
GGCAACAGCC GGCCTAGTAC ACCAACAGTC TCCACCGACA CCAAAGACAC GGACAGCGAG 
CAGGAGGGGA AAGACGACGA CGATAATGAT GATGATGATG ATGATGATGA TGACAAGAAG 
GACGAGACCA CCAGCAGTTC AGAGGGTAAC TCACGGTGCC AGACTCCAGT GAAGTTGAAG 
CTGACGTGTG ACCCTCAGGT TGTTGATTGG AGCGGAGCCG AGGCTTCACT CTTTAGGGTT 
CTCATCGGAA CCTACTACGA CAACTACTGC GCTATAGCAC GGCTCATAGG AACCAAGACC 
TGCAGACAGG TATATGAGTT CAGAGTGAAG GAGTCTAGTA TCATCGCTCG CGCTCCGGCC 
GAGGACGAAG ACACGCCCCC TCGCAAGAAG AAGAGGAAAC ACAGGTTGTG GGCCACTCAC 
TGCAGGAAGA TCCAACTGAA GAAAGATGGT TCGTCCAACC ACGTGTATAA CTACCAGCCA 
TGTGACCACC CCCGCCAGCC CTGTGACTCC TCCTGTCCCT GTGTCACTGC TCAGAACTTC 
TGTGAGAAGT TCTGCCAGTG CAGCTCAGAG TGTCAGAACC GTTTCCCAGG CTGCAGGTGT 
AAGGCCCAGT GTAACACTAA ACAGTGTCCG TGTTACCTGG CGGTCAGAGA GTGTGACCCT 
GACCTGTGTC TGACCTGCGG AGCCGCTGAA CACTGGGACA GCAAGAACGT CTCCTGTAAA 
AACTGCTCCA TACAGAGGGG AGCCAAGAAG CACCTGCTCC TAGCTCCGTC TGATGTAGCA 
GGCTGGGGCA TCTTCATCAA AGAGCCAGTT CAGAAGAATG AGTTCATCTC TGAGTACTGT 
GGGGAGATAA TCTCCCAAGA TGAGGCGGAT CGCAGAGGGA AGGTCTACGA CAAATACATG 
TGTAGCTTCC TCTTCAACCT CAACAACGAT TTTGTAGTGG ATGCTACTAG GAAAGGAAAC 
AAGATTCGTT TCGCCAACCA TTCCGTCCAC CCCAACTGCT ATGCAAAAGT GATGATGGTG 
AATGGGGACC ACAGGATAGG GATCTTTGCT AAGAGAACCA TCCAGACTGG AGAAGAGCTC 
TTCTTTGACT ACCGGTACAG TCAAGCAGAT GCTCTGAAGT ACGTCGGCAT CGAGCGGGAA 
TCGGAGATGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ezh2 ( XM_021620397.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021476072.1 Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X4, mRNA.

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CloneID	OOf56009
Clone ID Related Accession (Same CDS sequence)	XM_021620397.1
Accession Version	XM_021620397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	histone-lysine N-methyltransferase EZH2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA CAGGGAAGCG GTCTGAGAAG GGACCAGTGT GTTGGAGGAG ACGGGTGAAG 
TCTGAGTACA TGAGGCTACG CCAACTCAAA CGTTTCAGAC GGGCTGATGA GGTCAAGAGT 
ATGTTTAACT CTAACCGTCA GAGGATCCTG GAGCGGACAG ACATCCTGAA CGCTGAGTGG 
AAGACCAGAA GGATCCAGCC CGTTCACATC ATGACATCTG TTAGCTCCTT ACGAGGGACC 
AGAGAGTGCA CAGTGGACAG TGGTTTCTCT GAGTTCTCCA AACAGGTGAT ACCTCTGAAG 
ACGCTCAACG CTGTAGCCTC TGTCCCTGTC ATGTACTCCT GGTCCCCGCT ACAGCAGANN 
NNNNNNNNCA TGACATCTAA CTTCATGGTA GAAGACGAAA CGGTTCTCCA CAACATCCCT 
TACATGGGAG ACGAGATTCT GGACCAAGAC GGAACCTTCA TAGAAGAACT CATCAAGAAC 
TACGATGGCA AAGTTCACGG AGACAGGGAG TGCGGCTTCA TCAACGATGA GATCTTTGTG 
GAGCTGGTGG GTGCCCTGAC CCAGTACAGT GACAACGATG ATGAGGATGA TGATGAAGAA 
GAGCAGGAGT TTAAGCTTGA GAAGATGGAG CTCTGTGAGG CGAAGGACCA CCTGGCCGAG 
GACCCCCGAA AGGAACCCCT GATCAACACT GACAGCCAAG GCAGCAGTGA CAGCTCTAAG 
AAGTTTCCGT CTGATAAGAT CTTTGAAGCC ATCTCCTCAA TGTTCCCTGA TAAGGGCTCA 
ACAGAGGAAC TTCGAGAAAA GTACAAGGAG CTGACAGAGC CCCAGTTGCC TGGTGCGTTG 
CCTCCAGAGT GTACTCCTAA CATGGACGGT CCCAACGCTC GCTCCGTTCA GAGGGAACAG 
AGTCTACACT CCTTCCACAC ACTCTTCTGC CGACGCTGCT TCAAATACGA CTGCTTCCTG 
CACCCCTTCC ATGCGACTCC TAACACCTAT AAGCGTAAGA ACATGGAGAA TCTGGTAGAC 
AGTAAACCCT GTGGTGACGA GTGCTACATG TACCTGGTGA AGGATGGATT GGTGAGGGAG 
TATCCTGACG GCATGGCAAC CGAGAGGTCC AAGACCCCTT CCAAACGCCC AGTGAGCCGT 
CGCCGGGGAC GACCCATCGG CAACAGCCGG CCTAGTACAC CAACAGTCTC CACCGACACC 
AAAGACACGG ACAGCGAGCA GGAGGGGAAA GACGACGACG ATAATGATGA TGATGATGAT 
GATGATGATG ACAAGAAGGA CGAGACCACC AGCAGTTCAG AGGGTAACTC ACGGTGCCAG 
ACTCCAGTGA AGTTGAAGCT GACGTGTGAC CCTCAGGTTG TTGATTGGAG CGGAGCCGAG 
GCTTCACTCT TTAGGGTTCT CATCGGAACC TACTACGACA ACTACTGCGC TATAGCACGG 
CTCATAGGAA CCAAGACCTG CAGACAGGTA TATGAGTTCA GAGTGAAGGA GTCTAGTATC 
ATCGCTCGCG CTCCGGCCGA GGACGAAGAC ACGCCCCCTC GCAAGAAGAA GAGGAAACAC 
AGGTTGTGGG CCACTCACTG CAGGAAGATC CAACTGAAGA AAGATGGTTC GTCCAACCAC 
GTGTATAACT ACCAGCCATG TGACCACCCC CGCCAGCCCT GTGACTCCTC CTGTCCCTGT 
GTCACTGCTC AGAACTTCTG TGAGAAGTTC TGCCAGTGCA GCTCAGAGTG TCAGAACCGT 
TTCCCAGGCT GCAGGTGTAA GGCCCAGTGT AACACTAAAC AGTGTCCGTG TTACCTGGCG 
GTCAGAGAGT GTGACCCTGA CCTGTGTCTG ACCTGCGGAG CCGCTGAACA CTGGGACAGC 
AAGAACGTCT CCTGTAAAAA CTGCTCCATA CAGAGGGGAG CCAAGAAGCA CCTGCTCCTA 
GCTCCGTCTG ATGTAGCAGG CTGGGGCATC TTCATCAAAG AGCCAGTTCA GAAGAATGAG 
TTCATCTCTG AGTACTGTGG GGAGATAATC TCCCAAGATG AGGCGGATCG CAGAGGGAAG 
GTCTACGACA AATACATGTG TAGCTTCCTC TTCAACCTCA ACAACGATTT TGTAGTGGAT 
GCTACTAGGA AAGGAAACAA GATTCGTTTC GCCAACCATT CCGTCCACCC CAACTGCTAT 
GCAAAAGTGA TGATGGTGAA TGGGGACCAC AGGATAGGGA TCTTTGCTAA GAGAACCATC 
CAGACTGGAG AAGAGCTCTT CTTTGACTAC CGGTACAGTC AAGCAGATGC TCTGAAGTAC 
GTCGGCATCG AGCGGGAATC GGAGATGCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021476072.1
CDS	418..2730
Translation	MVLTGKRSEKGPVCWRRRVKSEYMRLRQLKRFRRADEVKSMFNSNRQRILERTDILNAEWKTRRIQPVHIMTSVSSLRGTRECTVDSGFSEFSKQVIPLKTLNAVASVPVMYSWSPLQQnullnullnullnullMTSNFMVEDETVLHNIPYMGDEILDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVGALTQYSDNDDEDDDEEEQEFKLEKMELCEAKDHLAEDPRKEPLINTDSQGSSDSSKKFPSDKIFEAISSMFPDKGSTEELREKYKELTEPQLPGALPPECTPNMDGPNARSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNMENLVDSKPCGDECYMYLVKDGLVREYPDGMATERSKTPSKRPVSRRRGRPIGNSRPSTPTVSTDTKDTDSEQEGKDDDDNDDDDDDDDDKKDETTSSSEGNSRCQTPVKLKLTCDPQVVDWSGAEASLFRVLIGTYYDNYCAIARLIGTKTCRQVYEFRVKESSIIARAPAEDEDTPPRKKKRKHRLWATHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVTAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAAEHWDSKNVSCKNCSIQRGAKKHLLLAPSDVAGWGIFIKEPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVHPNCYAKVMMVNGDHRIGIFAKRTIQTGEELFFDYRYSQADALKYVGIERESEMP

Target ORF information:

RefSeq Version	XM_021620397.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss enhancer of zeste 2 polycomb repressive complex 2 subunit (ezh2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021620397.1

ORF Insert Sequence:

ATGGTGCTGA CAGGGAAGCG GTCTGAGAAG GGACCAGTGT GTTGGAGGAG ACGGGTGAAG 
TCTGAGTACA TGAGGCTACG CCAACTCAAA CGTTTCAGAC GGGCTGATGA GGTCAAGAGT 
ATGTTTAACT CTAACCGTCA GAGGATCCTG GAGCGGACAG ACATCCTGAA CGCTGAGTGG 
AAGACCAGAA GGATCCAGCC CGTTCACATC ATGACATCTG TTAGCTCCTT ACGAGGGACC 
AGAGAGTGCA CAGTGGACAG TGGTTTCTCT GAGTTCTCCA AACAGGTGAT ACCTCTGAAG 
ACGCTCAACG CTGTAGCCTC TGTCCCTGTC ATGTACTCCT GGTCCCCGCT ACAGCAGANN 
NNNNNNNNCA TGACATCTAA CTTCATGGTA GAAGACGAAA CGGTTCTCCA CAACATCCCT 
TACATGGGAG ACGAGATTCT GGACCAAGAC GGAACCTTCA TAGAAGAACT CATCAAGAAC 
TACGATGGCA AAGTTCACGG AGACAGGGAG TGCGGCTTCA TCAACGATGA GATCTTTGTG 
GAGCTGGTGG GTGCCCTGAC CCAGTACAGT GACAACGATG ATGAGGATGA TGATGAAGAA 
GAGCAGGAGT TTAAGCTTGA GAAGATGGAG CTCTGTGAGG CGAAGGACCA CCTGGCCGAG 
GACCCCCGAA AGGAACCCCT GATCAACACT GACAGCCAAG GCAGCAGTGA CAGCTCTAAG 
AAGTTTCCGT CTGATAAGAT CTTTGAAGCC ATCTCCTCAA TGTTCCCTGA TAAGGGCTCA 
ACAGAGGAAC TTCGAGAAAA GTACAAGGAG CTGACAGAGC CCCAGTTGCC TGGTGCGTTG 
CCTCCAGAGT GTACTCCTAA CATGGACGGT CCCAACGCTC GCTCCGTTCA GAGGGAACAG 
AGTCTACACT CCTTCCACAC ACTCTTCTGC CGACGCTGCT TCAAATACGA CTGCTTCCTG 
CACCCCTTCC ATGCGACTCC TAACACCTAT AAGCGTAAGA ACATGGAGAA TCTGGTAGAC 
AGTAAACCCT GTGGTGACGA GTGCTACATG TACCTGGTGA AGGATGGATT GGTGAGGGAG 
TATCCTGACG GCATGGCAAC CGAGAGGTCC AAGACCCCTT CCAAACGCCC AGTGAGCCGT 
CGCCGGGGAC GACCCATCGG CAACAGCCGG CCTAGTACAC CAACAGTCTC CACCGACACC 
AAAGACACGG ACAGCGAGCA GGAGGGGAAA GACGACGACG ATAATGATGA TGATGATGAT 
GATGATGATG ACAAGAAGGA CGAGACCACC AGCAGTTCAG AGGGTAACTC ACGGTGCCAG 
ACTCCAGTGA AGTTGAAGCT GACGTGTGAC CCTCAGGTTG TTGATTGGAG CGGAGCCGAG 
GCTTCACTCT TTAGGGTTCT CATCGGAACC TACTACGACA ACTACTGCGC TATAGCACGG 
CTCATAGGAA CCAAGACCTG CAGACAGGTA TATGAGTTCA GAGTGAAGGA GTCTAGTATC 
ATCGCTCGCG CTCCGGCCGA GGACGAAGAC ACGCCCCCTC GCAAGAAGAA GAGGAAACAC 
AGGTTGTGGG CCACTCACTG CAGGAAGATC CAACTGAAGA AAGATGGTTC GTCCAACCAC 
GTGTATAACT ACCAGCCATG TGACCACCCC CGCCAGCCCT GTGACTCCTC CTGTCCCTGT 
GTCACTGCTC AGAACTTCTG TGAGAAGTTC TGCCAGTGCA GCTCAGAGTG TCAGAACCGT 
TTCCCAGGCT GCAGGTGTAA GGCCCAGTGT AACACTAAAC AGTGTCCGTG TTACCTGGCG 
GTCAGAGAGT GTGACCCTGA CCTGTGTCTG ACCTGCGGAG CCGCTGAACA CTGGGACAGC 
AAGAACGTCT CCTGTAAAAA CTGCTCCATA CAGAGGGGAG CCAAGAAGCA CCTGCTCCTA 
GCTCCGTCTG ATGTAGCAGG CTGGGGCATC TTCATCAAAG AGCCAGTTCA GAAGAATGAG 
TTCATCTCTG AGTACTGTGG GGAGATAATC TCCCAAGATG AGGCGGATCG CAGAGGGAAG 
GTCTACGACA AATACATGTG TAGCTTCCTC TTCAACCTCA ACAACGATTT TGTAGTGGAT 
GCTACTAGGA AAGGAAACAA GATTCGTTTC GCCAACCATT CCGTCCACCC CAACTGCTAT 
GCAAAAGTGA TGATGGTGAA TGGGGACCAC AGGATAGGGA TCTTTGCTAA GAGAACCATC 
CAGACTGGAG AAGAGCTCTT CTTTGACTAC CGGTACAGTC AAGCAGATGC TCTGAAGTAC 
GTCGGCATCG AGCGGGAATC GGAGATGCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ezh2 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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