LOC110531171 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110531171
Entrez Gene ID	110531171
Full Name	voltage-dependent L-type calcium channel subunit alpha-1D-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021614407.1	XP_021470082.1	voltage-dependent L-type calcium channel subunit alpha-1D-like

Pathways from BioSystems
Gene Ontology

The following LOC110531171 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110531171 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf06417	XM_021614407.1 Latest version!	Oncorhynchus mykiss voltage-dependent L-type calcium channel subunit alpha-1D-like (LOC110531171), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110531171 ( XM_021614407.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021470082.1 Oncorhynchus mykiss voltage-dependent L-type calcium channel subunit alpha-1D-like (LOC110531171), mRNA.

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CloneID	OOf06417
Clone ID Related Accession (Same CDS sequence)	XM_021614407.1
Accession Version	XM_021614407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	voltage-dependent L-type calcium channel subunit alpha-1D-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGCAAGAGG CAGAGGAACA GCCCGAAGGG GCACAGTATG CCAGCAGCAA CAGAGCCCAT 
CACTCTGGTG ACACGTCGGC CCTCGGGTCA GAAAACCCCT CCCACCCGCA GCCCGAACCC 
TGCCCCCAGA CCGGCACTAT CTCCTGGCAG GCCGCGATCC ATGCTGCTCG TAACGCCCAG 
GGCGAGGCAC CCCAGAGCAT GAGCGCCCGC CCCCCTGTCC CTGGGGCCAC ACCCGCCCCG 
GTGGGCTCAC TGTCACAGAG GAAGAGGCAG CTGTATGCCA AGAGCAAGAA GCAGGGCGGC 
TCCACCAACA GCAGACCGCC CAGGGCACTG TTCTGCCTCA CCCTCAACAA CCCCATCCGC 
AGAGCCTGCA TCAGCCTGGT GGAGTGGAAA CCCTTTGATA TCTTTATACT GCTCTCTATT 
TTTGCCAACT GTGTGGCCTT AGCTGTTTAC ATCCCCTTCC CAGAGGACGA CTCCAACTCC 
ACCAACCACG ACCTGGAAAC AGTAGAATAT GCCTTCCTAA TCATCTTTAC TATAGAGACC 
TTTCTGAAGA TCATAGCGTA TGGTTTAGTC ATGCACCAGA ACTCCTACGT AAGAAACGGA 
TGGAACATGC TGGATTTTGT CATTGTCATA GTGGGGTTGT TCAGTGTAGT TCTGGAGGTG 
ATAACCAAAG AGGGCGATGC CGGGGGCCAG GGGGGTAAGC CGGGAGGCTT CGACGTCAAG 
GCCCTCCGAG CATTCCGGGT GCTCCGCCCC CTCCGCCTTG TCTCAGGAGT ACCCAGTTTA 
CAGGTAGTGT TGAACTCCAT CATTAAAGCC ATGGTTCCTC TGCTCCACAT CGCTCTGCTC 
GTCCTCTTCG TCATCATCAT CTACGCCATC ATCGGCCTCG AACTCTTCAT CGGCAAGATG 
CACGCCACCT GCTACCTGGT GGGCACCGGT ATTCTGGCCG AGGAGGAGCC TGTTCCGTGT 
GCGGTGTCAG GCCATGGGCG CCAGTGCAGG ATGAATGGGA CTGTGTGTAG GGAAGGATGG 
CACGGGCCCA ATGGTGGCAT CACCAACTTT GACAACTTCC TGTTTGCCAT GCTCACTGTT 
TTCCAGTGTA TCACCATGGA GGGCTGGACT GATGTTCTGT ACTGGGTGAG GCTGCTTTAT 
GAACCACACC CAGAAACAGT AGAATATGCC TTCCTAATCA TCTTTACTAT AGAGACCTTT 
CTGAAGATCA TAGCGTATGG TTTAGTCATG CACCAGAACT CCTACGTAAG AAACGGATGG 
AACATGCTGG ATTTTGTCAT TGTCATAGTG GGAAACTCAG AGCCATCGTT CGATTGGATC 
TCACCTTACT TCCTGTGTTT GTGTGATGTC ACAGCAAGCG TGCCTGCCAG CGAGACCGAG 
TCTGTGAACA CTGACAACCC CAATGGAGAG GACGATAAGG CTAACTGCTG TGGACCACAC 
TGTCAAAAAA TTACTAAATC AAAGTACAGT CGGCGATGGC GTCACTGGAA CCGGTTCTGC 
CGCAGGAAGT GTCGTGCTGC CGTGAAGTCG GTGTCTTTCT ATTGGCTGGT GATCATCCTG 
GTGTTTCTGA ACACGCTCAC CATCTCGTCA GAGCACTACA ACCAGCCTGA CTGGCTCACG 
GAAGTACAGG ATGTGGCCAA CAAGGTTCTG CTGGCTTTGT TCACGTGTGA GATGCTGGTG 
AAGATGTACA GTCTGGGCCT GCAGGCCTAC TTCGTGTCCC TGTTCAACCG CTTCGACTGC 
TTCGTGGTGT GCGGCGGCAT CGTGGAGACC ATCCTGGTGG AGCTGGCCGT CATGTCTCCT 
CTGGGCATCT CTGTGTTCCG TTGTGTCCGC CTGCTACGCA TCTTCAAGGT CACACGCCAC 
TGGGCATCTC TCAGTAACCT GGTGGCGTCG CTGCTCAACT CCATGAAGTC CATCGCTTCC 
CTGCTGCTTC TGCTCTTCCT CTTCATCATC ATCTTCTCCC TGTTGGGCAT GCAGCTCTTC 
GGAGGCAAGT TCAACTTCGA CGAGACGATA ACCAAGAGGA GCACCTTCGA CAACTTCCCC 
CAGGCACTAC TCACAGTGTT TCAGATCCTG ACAGGCGAGG ATTGGAACGC TGTGATGTAT 
GACGGCATCA TGGCGTACGG TGGACCCTTC TCATCGGGCA TGGTCGTCAG CATCTACTTC 
ATCATACTCT TCGTCTGCGG AAACTACATC CTGCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAACCTGG CAGACGCAGA GAGCCTGAAC ACAGCCCAGA AGGAGGAAGA GGAGGAGAAG 
GAGAGGAAGA AGAGTTCCAG AAGTGACACA GACAAGACCG AGGATCTGAA ACCTGAAGGC 
CAGGATGAGG AGATCAAGCT TCCATTGGAG GAGGAAGTGG AAGAGGAGAA GGAGCCGTAT 
CCCACTCCAG ATACTCCAGT TGGTGACGAT GACGACGACG ACGATGAGCT CCCGGAGGTT 
CCGATTGGGC CACGTCCTCA GAGGCTGTCG GACCACAGCG TCAAGGAGAA GACTCCGCCC 
ATCCCTGAGG GCAGCGCTTT CTTCATCTTC AGCAACACTA ACCTAGTCCG GGTGGCCTGC 
CACAAGCTGA CCAACCACCA CATCTTCACC AACCTCATCC TGGTCTTCAT CATGCTGAGC 
TCTGTGTCTC TGGCTGCTGA GGACCCCATA CGCAACTTCT CCGCACGCAA CATTATACTT 
GGTTATTTTG ACTATGCTTT CACGGCAATC TTTACTGTTG AGATCCTGTT GAAGATCCTA 
GGCTATGCAG ATTATGTCTT CACTAGTATG TTTACCTTTG AGATCCTGTT GAAGATGACA 
GCGTTTGGGG CTTTCCTCCA TAAAGGAGCC TTCTGCAGGA ACTACTTCAA CCTGCTGGAC 
CTACTGGTGG TGGGAGTCTC TCTGGTGTCC TTCGGCATTC AGTCATCCGC CATCTCTGTG 
GTGAAGATCC TGCGTGTGCT GAGGGTGCTG CGGCCCCTGA GGGCCATCAA CAGAGCCAAG 
GGCCTCAAGC ATGTGGTCCA GTGTGTATTT GTGGCCATCA GGACCATTGG TAATATCATG 
ATCGTCACCA CGCTGCTACA GTTCATGTTC GCCTGCATAG GAGTGCAGCT CTTCAAGGGC 
AAGTTCTATC GCTGCACAGA CGAGGCCAAG TCCAGCCCAG AGGAATGCAA AGGCACATAC 
ATTCTGTATA AGGATGGCGA CACAGTGTTG CCTGCGGTCA GGGAGAGGAT CTGGTACAAC 
AGTGACTTCA ACTTTGACAA CGTCCTCATG GCTATGATGG CTCTGTTCAC CGTCTCTACA 
TTTGAAGGCT GGCCTGCTCT GCTGTACAAG GCCATCGACT CCAACAGAGA GAATATGGGT 
CCCATCTACA ACTACCGCGT GGAGATCTCC ATCTTCTTCA TCATCTACAT CATCATCATC 
GCCTTCTTCA TGATGAACAT CTTTGTCGGT TTCGTCATCG TCACCTTCCA GGAGCAGGGA 
GAGAAAGAGT ACAAGAACTG TGAGCTGGAC AAAAACCAGC GTCAGTGTGT AGAGTATGCT 
CTGAAGGCTC GTCCACTGCG GAGGTACATC CCTAAGAACC CCTACCAGTA TAAGTTCTGG 
TATGTGGTTA ACTCCACTGG CTTTGAGTAC GTCATGTTCG TTCTCATCAT CCTCAACACA 
CTGTGTTTGG CGATCCAGCA CTATGGCCAG TCCCACCTGT TCAACTACGC CATGGACGTC 
CTCAACATGG TCTTCACTGG CGTCTTCACC GTGGAGATGA TCCTCAAACT GATTGCCTTC 
AGACCCAGGG GGTATTTTGG AGACGCCTGG AACGTCTTCG ATGCCCTGGT GGTGATCGGC 
AGCGTAGTCG ACATCATTCT CAGTCAGGTA GATCACTATT TCGCCGATGC ATGGAACACG 
TTTGATGCCT TAATTGTTGT CGGTAGCGTC GTTGACATTG CAATCACTGA GATCAATGCC 
CTGCCAGTGG TGAAGGTGAT TGTGGAGCCA GGGAACACAG AGGACAGCGC TCGCATCTCC 
ATCACATTCT TCCGCTTGTT CCGAGTCATG CGATTGGTCA AGCTGCTCAG CAGAGGGGAG 
GGCATCCGAA CGCTCCTGTG GACCTTCATC AAGTCCTTCC AGGCTCTTCC CTATGTTGCC 
CTCCTGATAG CCATGCTATT CTTCATCTAC GCTGTTATCG GCATGCAGGT GTTTGGAAAG 
ATCGCCATGG TAGACGGCAC ACAGATAAAC CACAACAACA ACTTCCAGAC CTTTCCTCAA 
GCTGTCCTGA TGCTCTTCAG GTGTGCTACA GGCGAGGTGT GGCAGGAGAT CATGCTGGCA 
TGTCTGCCAG GGAAGCTGTG TGACTCCGAG TCAGACTACA ACCCCGGAGA GGAGAGAACC 
TGTGGAAGCG GATTCGCCAT CATCTACTTC ATCAGCTTCT ACATGCTCTG CGCCTTCCTG 
ATCATCAACC TGTTCGTGGC TGTCATCATG GATAACTTTG ACTATCTGAC GCGGGATTGG 
TCCATTCTGG GTCCTCACCA CCTGGATGAG TTTAAAAGGA TCTGGTCTGA GTACGACCCG 
GAGGCTAAGG GCAGGATAAA GCATCTGGAT GTGGTGACCT TGCTGAGACG CATCCAGCCT 
CCTCTAGGCT TCGGCAAGCT GTGTCCACAC AGAGTGGCCT GCAAGAGATT GGTTGCCATG 
AACATGCCCC TGAACAGTGA TGGCACGGTC ATGTTTAACG CCACCCTGTT TGCCTTGGTG 
AGGACCGCTC TGAAGATCAA GACCGAAGGC AACCTGGAGC AGGCCAATGA GGAGTTGCGA 
GCGGTCATCA AGAAGATCTG GAAGAGGACC AGCATGAAGC TGCTGGACCA AGTGGTGCCC 
CCTGCTGGCG ATGACGAGGT AACCGTGGGG AAGTTCTATG CTACCTTCCT GATACAGGAT 
TACTTCAGAA AATTCAAGAA ACGCAAAGAG CAAGGCCTGG TGGGAAGAAA CTCATTCACC 
AGCCTGAACT CCACCATCGC CCTCCAGGCC GGTCTCCGCA CACTGCATGA TATTGGGCCT 
GAAATCCGCC GAGTCATATC ATGTGACCTG CAAGACGAGG GCCTTGTCGA CATTAACGCA 
GAAGACGGCG AGGAGATTTA CAGGAGGAAC GGCGTGATCT TCTCCAACCA CACCAATCAC 
GTTAACGGGG ACCACCGGGG TTCCGCCCAC CAGACAAACG TCACTCAGCG GCCCCTCCAG 
GTCCCTCCGC CTTCCTGCCA CGCCTCCGTG GATCCCTCCC AGGTCGGCTC TGAGGAAAGG 
GCAGGCGAAC CCCACTGGTA CAACTACTAC TACCCCAAAT CCCCCATATC CTCAAACGCC 
AACCTTAACA ATGCCAACGT GCACCCTGTG CCCTCCCTGA CAAACGGGGG GCAGCGCCGC 
TGTCTTCGCC GTGAGCACCC GTCTGCTAAT GGGGCACCCT CTTCCAGCTC GCACCGCAAG 
ATTGTTGACT TCGACACCCA CACCACGGTC CAATCTGCGA GGTCAGATTC AGGGGGTGTT 
CGCTACCCTA CCGTTCGTAG AGAGGAGCCG GGGAGGGCAG ACAGTGATGA AGACAGGGGC 
TCTGGGGAAT ACTTCAGCGG GGAAGAGTAC CAGGAGGATG ATATCATGTT GCACAAAGAC 
AGGATGTCCC GTAAGGAGTG TCAAGACCAT CAACCGGACA GTCTCTGTGA TGACCACCAG 
CAACCCATTT ACCAGGATGG TGGGCGGTCG CCGAAGCGAT GGTTTCTCCC CTCTACACCC 
CAGGCCCTCC ACAGACCCTC ATTCAACTTT CAATGTCTGC GGAAACAAAG CAGTCAAGGC 
GATGACACCC CCTTGACCCC TTCCGTTCCC CTTCGAACAG CTCTACCCCT GCATCTGCTG 
CAGCAGCAGG TGATGGCGGT GGCTGGCCTG GACTCCAGCA GAGTCCCGCG TCTCTCTCCG 
TCACGCTCTC TACGCTCCTG GGCCACGCCC CCCGTCTCCC CCAGCAGCAG GGACCACTCC 
CATTACTACA CACCCCTCAT CCAGGTGGAC TGGCGTGGGC TGAGCAGTGT GAGTAGCACC 
CCTGGCTCTG TGAGAAGGAG CACCTGGTAC ACTGACGTCC AAGAAGCCCC CCTAAGCCGT 
ACTTACTCCC CCTTACAGCT CCAGATCCCA CCTGAATATC GCAGGCAGTA CCACCAGAAC 
AGAGGCAGTG CTAACAGCCT AGTGGAGGCT GTGTTGATTT CAGAAGGCCT GGGGAAATAC 
GCCAAGGACC CCAAGTTTGT GTCGGCGACC AAGCACGAGA TCGCGGACGC CTGCGAGATG 
ACCATCGACG AGATGGAGAG TGCCGCCAGC AACCTGCTGA ACGGCAGTAT GGGTAATGGG 
GCGGGGACAA CGGACACAGT GACTGGCACG AGCCTCATGG ACCGCAATCT CGAGGACTAC 
AGCGATGAGG AGCCGGACAC GTGCGGGAAA TACGAGGAGG ATCTGACTGA TGAGATGATA 
TGCATCAGCA CCCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021470082.1
CDS	315..6812
Translation	MQEAEEQPEGAQYASSNRAHHSGDTSALGSENPSHPQPEPCPQTGTISWQAAIHAARNAQGEAPQSMSARPPVPGATPAPVGSLSQRKRQLYAKSKKQGGSTNSRPPRALFCLTLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNSYVRNGWNMLDFVIVIVGLFSVVLEVITKEGDAGGQGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCYLVGTGILAEEEPVPCAVSGHGRQCRMNGTVCREGWHGPNGGITNFDNFLFAMLTVFQCITMEGWTDVLYWVRLLYEPHPETVEYAFLIIFTIETFLKIIAYGLVMHQNSYVRNGWNMLDFVIVIVGNSEPSFDWISPYFLCLCDVTASVPASETESVNTDNPNGEDDKANCCGPHCQKITKSKYSRRWRHWNRFCRRKCRAAVKSVSFYWLVIILVFLNTLTISSEHYNQPDWLTEVQDVANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELAVMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETITKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPFSSGMVVSIYFIILFVCGNYILLNVFLAIAVDNLADAESLNTAQKEEEEEKERKKSSRSDTDKTEDLKPEGQDEEIKLPLEEEVEEEKEPYPTPDTPVGDDDDDDDELPEVPIGPRPQRLSDHSVKEKTPPIPEGSAFFIFSNTNLVRVACHKLTNHHIFTNLILVFIMLSSVSLAAEDPIRNFSARNIILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEILLKMTAFGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSSPEECKGTYILYKDGDTVLPAVRERIWYNSDFNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNRENMGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYVMFVLIILNTLCLAIQHYGQSHLFNYAMDVLNMVFTGVFTVEMILKLIAFRPRGYFGDAWNVFDALVVIGSVVDIILSQVDHYFADAWNTFDALIVVGSVVDIAITEINALPVVKVIVEPGNTEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKIAMVDGTQINHNNNFQTFPQAVLMLFRCATGEVWQEIMLACLPGKLCDSESDYNPGEERTCGSGFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGRNSFTSLNSTIALQAGLRTLHDIGPEIRRVISCDLQDEGLVDINAEDGEEIYRRNGVIFSNHTNHVNGDHRGSAHQTNVTQRPLQVPPPSCHASVDPSQVGSEERAGEPHWYNYYYPKSPISSNANLNNANVHPVPSLTNGGQRRCLRREHPSANGAPSSSSHRKIVDFDTHTTVQSARSDSGGVRYPTVRREEPGRADSDEDRGSGEYFSGEEYQEDDIMLHKDRMSRKECQDHQPDSLCDDHQQPIYQDGGRSPKRWFLPSTPQALHRPSFNFQCLRKQSSQGDDTPLTPSVPLRTALPLHLLQQQVMAVAGLDSSRVPRLSPSRSLRSWATPPVSPSSRDHSHYYTPLIQVDWRGLSSVSSTPGSVRRSTWYTDVQEAPLSRTYSPLQLQIPPEYRRQYHQNRGSANSLVEAVLISEGLGKYAKDPKFVSATKHEIADACEMTIDEMESAASNLLNGSMGNGAGTTDTVTGTSLMDRNLEDYSDEEPDTCGKYEEDLTDEMICISTL

Target ORF information:

RefSeq Version	XM_021614407.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss voltage-dependent L-type calcium channel subunit alpha-1D-like (LOC110531171), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021614407.1

ORF Insert Sequence:

ATGCAAGAGG CAGAGGAACA GCCCGAAGGG GCACAGTATG CCAGCAGCAA CAGAGCCCAT 
CACTCTGGTG ACACGTCGGC CCTCGGGTCA GAAAACCCCT CCCACCCGCA GCCCGAACCC 
TGCCCCCAGA CCGGCACTAT CTCCTGGCAG GCCGCGATCC ATGCTGCTCG TAACGCCCAG 
GGCGAGGCAC CCCAGAGCAT GAGCGCCCGC CCCCCTGTCC CTGGGGCCAC ACCCGCCCCG 
GTGGGCTCAC TGTCACAGAG GAAGAGGCAG CTGTATGCCA AGAGCAAGAA GCAGGGCGGC 
TCCACCAACA GCAGACCGCC CAGGGCACTG TTCTGCCTCA CCCTCAACAA CCCCATCCGC 
AGAGCCTGCA TCAGCCTGGT GGAGTGGAAA CCCTTTGATA TCTTTATACT GCTCTCTATT 
TTTGCCAACT GTGTGGCCTT AGCTGTTTAC ATCCCCTTCC CAGAGGACGA CTCCAACTCC 
ACCAACCACG ACCTGGAAAC AGTAGAATAT GCCTTCCTAA TCATCTTTAC TATAGAGACC 
TTTCTGAAGA TCATAGCGTA TGGTTTAGTC ATGCACCAGA ACTCCTACGT AAGAAACGGA 
TGGAACATGC TGGATTTTGT CATTGTCATA GTGGGGTTGT TCAGTGTAGT TCTGGAGGTG 
ATAACCAAAG AGGGCGATGC CGGGGGCCAG GGGGGTAAGC CGGGAGGCTT CGACGTCAAG 
GCCCTCCGAG CATTCCGGGT GCTCCGCCCC CTCCGCCTTG TCTCAGGAGT ACCCAGTTTA 
CAGGTAGTGT TGAACTCCAT CATTAAAGCC ATGGTTCCTC TGCTCCACAT CGCTCTGCTC 
GTCCTCTTCG TCATCATCAT CTACGCCATC ATCGGCCTCG AACTCTTCAT CGGCAAGATG 
CACGCCACCT GCTACCTGGT GGGCACCGGT ATTCTGGCCG AGGAGGAGCC TGTTCCGTGT 
GCGGTGTCAG GCCATGGGCG CCAGTGCAGG ATGAATGGGA CTGTGTGTAG GGAAGGATGG 
CACGGGCCCA ATGGTGGCAT CACCAACTTT GACAACTTCC TGTTTGCCAT GCTCACTGTT 
TTCCAGTGTA TCACCATGGA GGGCTGGACT GATGTTCTGT ACTGGGTGAG GCTGCTTTAT 
GAACCACACC CAGAAACAGT AGAATATGCC TTCCTAATCA TCTTTACTAT AGAGACCTTT 
CTGAAGATCA TAGCGTATGG TTTAGTCATG CACCAGAACT CCTACGTAAG AAACGGATGG 
AACATGCTGG ATTTTGTCAT TGTCATAGTG GGAAACTCAG AGCCATCGTT CGATTGGATC 
TCACCTTACT TCCTGTGTTT GTGTGATGTC ACAGCAAGCG TGCCTGCCAG CGAGACCGAG 
TCTGTGAACA CTGACAACCC CAATGGAGAG GACGATAAGG CTAACTGCTG TGGACCACAC 
TGTCAAAAAA TTACTAAATC AAAGTACAGT CGGCGATGGC GTCACTGGAA CCGGTTCTGC 
CGCAGGAAGT GTCGTGCTGC CGTGAAGTCG GTGTCTTTCT ATTGGCTGGT GATCATCCTG 
GTGTTTCTGA ACACGCTCAC CATCTCGTCA GAGCACTACA ACCAGCCTGA CTGGCTCACG 
GAAGTACAGG ATGTGGCCAA CAAGGTTCTG CTGGCTTTGT TCACGTGTGA GATGCTGGTG 
AAGATGTACA GTCTGGGCCT GCAGGCCTAC TTCGTGTCCC TGTTCAACCG CTTCGACTGC 
TTCGTGGTGT GCGGCGGCAT CGTGGAGACC ATCCTGGTGG AGCTGGCCGT CATGTCTCCT 
CTGGGCATCT CTGTGTTCCG TTGTGTCCGC CTGCTACGCA TCTTCAAGGT CACACGCCAC 
TGGGCATCTC TCAGTAACCT GGTGGCGTCG CTGCTCAACT CCATGAAGTC CATCGCTTCC 
CTGCTGCTTC TGCTCTTCCT CTTCATCATC ATCTTCTCCC TGTTGGGCAT GCAGCTCTTC 
GGAGGCAAGT TCAACTTCGA CGAGACGATA ACCAAGAGGA GCACCTTCGA CAACTTCCCC 
CAGGCACTAC TCACAGTGTT TCAGATCCTG ACAGGCGAGG ATTGGAACGC TGTGATGTAT 
GACGGCATCA TGGCGTACGG TGGACCCTTC TCATCGGGCA TGGTCGTCAG CATCTACTTC 
ATCATACTCT TCGTCTGCGG AAACTACATC CTGCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAACCTGG CAGACGCAGA GAGCCTGAAC ACAGCCCAGA AGGAGGAAGA GGAGGAGAAG 
GAGAGGAAGA AGAGTTCCAG AAGTGACACA GACAAGACCG AGGATCTGAA ACCTGAAGGC 
CAGGATGAGG AGATCAAGCT TCCATTGGAG GAGGAAGTGG AAGAGGAGAA GGAGCCGTAT 
CCCACTCCAG ATACTCCAGT TGGTGACGAT GACGACGACG ACGATGAGCT CCCGGAGGTT 
CCGATTGGGC CACGTCCTCA GAGGCTGTCG GACCACAGCG TCAAGGAGAA GACTCCGCCC 
ATCCCTGAGG GCAGCGCTTT CTTCATCTTC AGCAACACTA ACCTAGTCCG GGTGGCCTGC 
CACAAGCTGA CCAACCACCA CATCTTCACC AACCTCATCC TGGTCTTCAT CATGCTGAGC 
TCTGTGTCTC TGGCTGCTGA GGACCCCATA CGCAACTTCT CCGCACGCAA CATTATACTT 
GGTTATTTTG ACTATGCTTT CACGGCAATC TTTACTGTTG AGATCCTGTT GAAGATCCTA 
GGCTATGCAG ATTATGTCTT CACTAGTATG TTTACCTTTG AGATCCTGTT GAAGATGACA 
GCGTTTGGGG CTTTCCTCCA TAAAGGAGCC TTCTGCAGGA ACTACTTCAA CCTGCTGGAC 
CTACTGGTGG TGGGAGTCTC TCTGGTGTCC TTCGGCATTC AGTCATCCGC CATCTCTGTG 
GTGAAGATCC TGCGTGTGCT GAGGGTGCTG CGGCCCCTGA GGGCCATCAA CAGAGCCAAG 
GGCCTCAAGC ATGTGGTCCA GTGTGTATTT GTGGCCATCA GGACCATTGG TAATATCATG 
ATCGTCACCA CGCTGCTACA GTTCATGTTC GCCTGCATAG GAGTGCAGCT CTTCAAGGGC 
AAGTTCTATC GCTGCACAGA CGAGGCCAAG TCCAGCCCAG AGGAATGCAA AGGCACATAC 
ATTCTGTATA AGGATGGCGA CACAGTGTTG CCTGCGGTCA GGGAGAGGAT CTGGTACAAC 
AGTGACTTCA ACTTTGACAA CGTCCTCATG GCTATGATGG CTCTGTTCAC CGTCTCTACA 
TTTGAAGGCT GGCCTGCTCT GCTGTACAAG GCCATCGACT CCAACAGAGA GAATATGGGT 
CCCATCTACA ACTACCGCGT GGAGATCTCC ATCTTCTTCA TCATCTACAT CATCATCATC 
GCCTTCTTCA TGATGAACAT CTTTGTCGGT TTCGTCATCG TCACCTTCCA GGAGCAGGGA 
GAGAAAGAGT ACAAGAACTG TGAGCTGGAC AAAAACCAGC GTCAGTGTGT AGAGTATGCT 
CTGAAGGCTC GTCCACTGCG GAGGTACATC CCTAAGAACC CCTACCAGTA TAAGTTCTGG 
TATGTGGTTA ACTCCACTGG CTTTGAGTAC GTCATGTTCG TTCTCATCAT CCTCAACACA 
CTGTGTTTGG CGATCCAGCA CTATGGCCAG TCCCACCTGT TCAACTACGC CATGGACGTC 
CTCAACATGG TCTTCACTGG CGTCTTCACC GTGGAGATGA TCCTCAAACT GATTGCCTTC 
AGACCCAGGG GGTATTTTGG AGACGCCTGG AACGTCTTCG ATGCCCTGGT GGTGATCGGC 
AGCGTAGTCG ACATCATTCT CAGTCAGGTA GATCACTATT TCGCCGATGC ATGGAACACG 
TTTGATGCCT TAATTGTTGT CGGTAGCGTC GTTGACATTG CAATCACTGA GATCAATGCC 
CTGCCAGTGG TGAAGGTGAT TGTGGAGCCA GGGAACACAG AGGACAGCGC TCGCATCTCC 
ATCACATTCT TCCGCTTGTT CCGAGTCATG CGATTGGTCA AGCTGCTCAG CAGAGGGGAG 
GGCATCCGAA CGCTCCTGTG GACCTTCATC AAGTCCTTCC AGGCTCTTCC CTATGTTGCC 
CTCCTGATAG CCATGCTATT CTTCATCTAC GCTGTTATCG GCATGCAGGT GTTTGGAAAG 
ATCGCCATGG TAGACGGCAC ACAGATAAAC CACAACAACA ACTTCCAGAC CTTTCCTCAA 
GCTGTCCTGA TGCTCTTCAG GTGTGCTACA GGCGAGGTGT GGCAGGAGAT CATGCTGGCA 
TGTCTGCCAG GGAAGCTGTG TGACTCCGAG TCAGACTACA ACCCCGGAGA GGAGAGAACC 
TGTGGAAGCG GATTCGCCAT CATCTACTTC ATCAGCTTCT ACATGCTCTG CGCCTTCCTG 
ATCATCAACC TGTTCGTGGC TGTCATCATG GATAACTTTG ACTATCTGAC GCGGGATTGG 
TCCATTCTGG GTCCTCACCA CCTGGATGAG TTTAAAAGGA TCTGGTCTGA GTACGACCCG 
GAGGCTAAGG GCAGGATAAA GCATCTGGAT GTGGTGACCT TGCTGAGACG CATCCAGCCT 
CCTCTAGGCT TCGGCAAGCT GTGTCCACAC AGAGTGGCCT GCAAGAGATT GGTTGCCATG 
AACATGCCCC TGAACAGTGA TGGCACGGTC ATGTTTAACG CCACCCTGTT TGCCTTGGTG 
AGGACCGCTC TGAAGATCAA GACCGAAGGC AACCTGGAGC AGGCCAATGA GGAGTTGCGA 
GCGGTCATCA AGAAGATCTG GAAGAGGACC AGCATGAAGC TGCTGGACCA AGTGGTGCCC 
CCTGCTGGCG ATGACGAGGT AACCGTGGGG AAGTTCTATG CTACCTTCCT GATACAGGAT 
TACTTCAGAA AATTCAAGAA ACGCAAAGAG CAAGGCCTGG TGGGAAGAAA CTCATTCACC 
AGCCTGAACT CCACCATCGC CCTCCAGGCC GGTCTCCGCA CACTGCATGA TATTGGGCCT 
GAAATCCGCC GAGTCATATC ATGTGACCTG CAAGACGAGG GCCTTGTCGA CATTAACGCA 
GAAGACGGCG AGGAGATTTA CAGGAGGAAC GGCGTGATCT TCTCCAACCA CACCAATCAC 
GTTAACGGGG ACCACCGGGG TTCCGCCCAC CAGACAAACG TCACTCAGCG GCCCCTCCAG 
GTCCCTCCGC CTTCCTGCCA CGCCTCCGTG GATCCCTCCC AGGTCGGCTC TGAGGAAAGG 
GCAGGCGAAC CCCACTGGTA CAACTACTAC TACCCCAAAT CCCCCATATC CTCAAACGCC 
AACCTTAACA ATGCCAACGT GCACCCTGTG CCCTCCCTGA CAAACGGGGG GCAGCGCCGC 
TGTCTTCGCC GTGAGCACCC GTCTGCTAAT GGGGCACCCT CTTCCAGCTC GCACCGCAAG 
ATTGTTGACT TCGACACCCA CACCACGGTC CAATCTGCGA GGTCAGATTC AGGGGGTGTT 
CGCTACCCTA CCGTTCGTAG AGAGGAGCCG GGGAGGGCAG ACAGTGATGA AGACAGGGGC 
TCTGGGGAAT ACTTCAGCGG GGAAGAGTAC CAGGAGGATG ATATCATGTT GCACAAAGAC 
AGGATGTCCC GTAAGGAGTG TCAAGACCAT CAACCGGACA GTCTCTGTGA TGACCACCAG 
CAACCCATTT ACCAGGATGG TGGGCGGTCG CCGAAGCGAT GGTTTCTCCC CTCTACACCC 
CAGGCCCTCC ACAGACCCTC ATTCAACTTT CAATGTCTGC GGAAACAAAG CAGTCAAGGC 
GATGACACCC CCTTGACCCC TTCCGTTCCC CTTCGAACAG CTCTACCCCT GCATCTGCTG 
CAGCAGCAGG TGATGGCGGT GGCTGGCCTG GACTCCAGCA GAGTCCCGCG TCTCTCTCCG 
TCACGCTCTC TACGCTCCTG GGCCACGCCC CCCGTCTCCC CCAGCAGCAG GGACCACTCC 
CATTACTACA CACCCCTCAT CCAGGTGGAC TGGCGTGGGC TGAGCAGTGT GAGTAGCACC 
CCTGGCTCTG TGAGAAGGAG CACCTGGTAC ACTGACGTCC AAGAAGCCCC CCTAAGCCGT 
ACTTACTCCC CCTTACAGCT CCAGATCCCA CCTGAATATC GCAGGCAGTA CCACCAGAAC 
AGAGGCAGTG CTAACAGCCT AGTGGAGGCT GTGTTGATTT CAGAAGGCCT GGGGAAATAC 
GCCAAGGACC CCAAGTTTGT GTCGGCGACC AAGCACGAGA TCGCGGACGC CTGCGAGATG 
ACCATCGACG AGATGGAGAG TGCCGCCAGC AACCTGCTGA ACGGCAGTAT GGGTAATGGG 
GCGGGGACAA CGGACACAGT GACTGGCACG AGCCTCATGG ACCGCAATCT CGAGGACTAC 
AGCGATGAGG AGCCGGACAC GTGCGGGAAA TACGAGGAGG ATCTGACTGA TGAGATGATA 
TGCATCAGCA CCCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC110531171 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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