LOC110495881 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110495881
Entrez Gene ID	110495881
Full Name	protein Shroom2-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021571391.1	XP_021427066.1	protein Shroom2-like isoform X6
XM_021571387.1	XP_021427062.1	protein Shroom2-like isoform X4
XM_021571389.1	XP_021427064.1	protein Shroom2-like isoform X5
XM_021571392.1	XP_021427067.1	protein Shroom2-like isoform X6
XM_021571384.1	XP_021427059.1	protein Shroom2-like isoform X1
XM_021571386.1	XP_021427061.1	protein Shroom2-like isoform X3
XM_021571385.1	XP_021427060.1	protein Shroom2-like isoform X2
XM_021571390.1	XP_021427065.1	protein Shroom2-like isoform X5

Pathways from BioSystems
Gene Ontology

The following LOC110495881 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110495881 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOf19771	XM_021571384.1 Latest version!	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf19772	XM_021571385.1 Latest version!	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf19773	XM_021571386.1 Latest version!	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf19774	XM_021571387.1 Latest version!	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf19775	XM_021571390.1 Latest version!	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOf19775	XM_021571389.1 Latest version!	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOf19776	XM_021571392.1 Latest version!	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOf19776	XM_021571391.1 Latest version!	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110495881 ( XM_021571384.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021427059.1 Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X1, mRNA.

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CloneID	OOf19771
Clone ID Related Accession (Same CDS sequence)	XM_021571384.1
Accession Version	XM_021571384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	protein Shroom2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTCG TCGACTACAG AAGCGAGTTA AGGATGTCAG CCAGAGAAGT CAAAGCCTTC 
CTAGGCGCGG ACATATTCAC GGCTGTCGGG GACAGGAATA AGGATGATGT TGAATATAGA 
TTTGTGGATG TCATGCTGTT TGGTGGGGCC CCTTGGGGGT TCACTCTGAG AGGGGGCCTG 
GAGCACCGAG AACCACTGCT CATAACCAAG GTGGAGGAGG GCAGCAAGGC GGCGACAGTC 
AGCCTGCAGG TGGGAGATGA GATCGTCAAC ATCAACACAG TTCCCATCAG TGGATCCAGG 
CAGGAGGCCG TCTGTCTGGT CAAGAGCTCC CACAAGACCC TCGCCCTGGT TGTCCGAAGG 
AGGAATGAAC CAGTCTCCCG TCCCCATTCA TGGCACTCCT CCAAGTTTAC AGAAGACCAC 
CCCGAACCCA CAGAGAGTCA CAACGAGCCT CCACCTGCAC CCCCACCTGT GTGGCAGGTC 
AAACATGAAG TCAGTGCATC CACAAAAGAC CTTTCCAGCT GCGGGGACCA CGACTCGAAT 
CTACGCCAGT TATCCAGCCA GTTCAGCTCC GTGGGGAATA TGGAGAGAGT AGATCGCCCC 
TCACACCCCT TCCAACCAGG ATGCCTCTCC CCCAGCAGGT ACCACCGCAG CGCTGAGCCT 
CTCTCTGGGG GTGGAGGGTC TGGTGGGAAG AGAGAATCGG CCTTCAGTTG CCTCTCCTCC 
ACCAGCCCTC CCCCGGAGCC TGCTCTGGCC AACACTGACA CTGCTGCAAC TGAGAGCAGC 
ATGTTCCATA AGGGGGTCCA GACTCAGACC AGTGAGGATG GAAGGCCGGG TGAGCAGCGC 
CACAGCCGGT ACCTCCAGCG GCCCCAAGGG GACGGAGGCT CGGAGAGCCC CAGGACAGTC 
CCAGAGGAGC AGCCTGGCTC CCGCTACTCC AGTTCAGGCT CTGGCAGATC GCACATAGGC 
CCTGTGTGGC ATGTCCCAGA GAGGAGGAGG GTAGCAGCGC CCCCCTCTCC TCCCCTGCGC 
AGTGACAGCT TTGCTGTCAC CAAGGTGTAC CCTGCCTACA CAGAGGGGCC TGGAGCTCCA 
CCACACGGGC AGATGAAGGC CCAGGACAGG TTGGCAGAGG CCCCAGAGAA CAGCAGCTAC 
AGAGGCCGGG GCAACCATAT CTCTCACGAT GAGAACGGTG TAGACCCTAG GTGCAGTTAC 
AACCCTCCAC CTTGCAAGAA AGACTTCTTC CACCCAAACA TAGCAGCAGG AGCACCTGAC 
TACAACCACA ACCAGCTCAG CAACCCAAAC AAACTGTCCT CTGTGTCTAG CCAGGACGTG 
AGACAGAGTC AGTCTCCCTT CACTTGCCTG CCCAACCACC AGCGGCAGTA TAGCGACGAA 
AGCACTTTCT ACCTGCAGAC CAGATCCGCC CCACATCCAC CGAAGCCGCA GAGCGTCGGT 
AGCTACTACC ACAGCCTCCA GGAGCTCCCT ACCAACTGCA CTAACAGCAG GAACCACGTG 
AGGTCCTCCA CCACGTCCCT GTCCACCTCG ACCATCGACC AGAACTATGA CTGCGGAGGA 
CACATCCGGT ACTACTGCAT CACAACCAAG CAGCCAGCCC AGCCAGAGAC TCATGTTAGA 
CAGAGCAAAT CAGAGGTCTG GAGATCTGAC ATGGAGCTAG CTAAGGGCTC CAACGACAGG 
GGCTCTACAA GTTCCTCCCA CAAGACCAAC AAGGTCAAGT ATCCTCCTAA TCCTCAGCCT 
GCTTACACCA ACAGCAAGGA GCGGAACGGA AATTTCCAAC CGGCCAATGT TCTGCTTTAC 
GGCCACACCA CCTCTAATTC CCTATCCTCA TCTGCTAAAC CTACCACTGA GGAGAATGAG 
AGGAAGAGTG AGGGCCAGAG ACCTACAGAG GCCCAGCTTA GAAGTTCTTA CTCTCCCAGT 
CCGCCCAAGG ACCACAAGGT GGGACCACTG AGAGAAGACC CTTGGGTCTC TCAGGAGAAC 
CATAAGATCT CTTCTCAAAA GACACCCATG CTCCACAGTC TGGCTCAGGA GAGTAGAAGC 
CTAGCCCAGG AGATTAGGAG CCCAATGCTT CATTCTCTAG CCCAGGAGAG TAAGAGCCTG 
GTGGAGAACA ATCACCCTGC TACGGCACAA CCACCACCAT CCAACGGCGG GGGAGAAGCC 
AACCACGCCC TACAGGAGGC TTTAACGGAC AACACGGCAA CGGGCAAACT GGCGAGACGC 
AGCGACCGAT ACGCCACCAC CCTCCGCAAC GAGATCCAGC TAAAGAGGGC CCAGCTGCAG 
AAAAGCCGGA GTGCTGCCAC CCTGACCTGC CCCAGCGAGA CGGAGGAGCC AGAGGAAGAG 
GAGACAGACC CGGGGGGGTG GAAGTCCACC TCCTCCACCT CCTCTGATGG CTCCTTTTCC 
ACGTCCTACA AGGACCACCT CAAAGAGGCG CAGGCGAGGG TCCTCCAGGC CACGTCCTTT 
AGGAGGAGAG ACCTAGAACC TCCCGGGTCA GGGTCGGAGG CTCCGTTAAC CAAACCCAAC 
TCACACATAG TCTCCCGCAT TGGCAGCCGC AAGCGTTTCC CTCTGAACAA GAGGGTCCAC 
TCGTTCTCCG AGCCAGACAA GATCAACAAG CTCGGAGTGG AGGGTGAGGG AGAACATCCT 
GTTGGAACAG CACGGTCATT TATAGACCGG CGGAAGTTCT TTGAAATGGC TGCTAAACCT 
TCCTTCTGCA GACCCATCTC AACCATCCAC AAGTCAGGCC AGCAGAGCTC CAGCACCACC 
TCTGGCACTT TGGAGCGCGG AGAGGGCAAG GCCAGAGGGA GAGCCCACTC AGGCGAAACT 
GAGGAGGGCT GGAGGCCAGG CCTTGCCAAT GAGACTTCCA GTGACCACCA CCCAGACCCC 
CTCAGCCCCG CAGGCAGACA GGCCCTACTG GAGCAGCAGA GGCTGGGGAC CTTCGCTGAG 
TACCAGGTCA CCTGGAACAT GCAGAGGAAG GCTTCAGACA CAAAGCCCCA GGGGAGGTAC 
CACTCTGCTG ACAACATCCT GGACCAGGGT ACAGAGGAGA CGCCTGTCTG TGTCCATGAG 
AGGTCCAGAT CGTCTCCCTC ACAAGACTTC CACACACAGA AGATCCCTAT GCCATGGAGA 
GAGACTGTTG AAAACCCGTA TCTGGATCAC AGACGGGACC AGCAAGGAGG CAGTTACACC 
GCCAGAGGGC AGAGCGAGAG CAGAGGGCAG CCAGCCCAGC TCCACCACTT CCCACAGCCT 
CCACAACCGT CTATTCCAAG ACTGACCGAC ACAGAGCCAC ACAGCAGCAG AGACGTGGCC 
ACCCCCACCC CCCTCCCTCT CCCCTCCGAA CACAGATACA AACGTGACTC TGCGAACCCC 
GCCCACAACC TCCCCGCTCT CCCCCCGTAC CCCTCCAATC ACACCCACAG CTCTGTGTCT 
GAGCAACCAC TACCACCTCC AACGCCCGCT CCCAAGCCCC AGAATACAGG CCTCATCATC 
ATGCCACATT GGCCTCTCCT AGGCCTGGAA ACCTCAACCA CATCCTCCTC CTACGGACTG 
TCTCCCCAGG AATTCCTGCC TGGCCCTTGC ACCCATCAGG CTGAACCATC ATCCAGCAGC 
AGCTGCTCCT CCCATGGCAC AGAGCTGGAT CCTGCCCCAA ACCAAGCCTG CTCCTTGGGC 
TCCACCCAGC TCCCCTCTCC CTCCCCTCGG AGAACCACTG GACTGGGCAC GGAGGAGGGA 
GCAGCTGATT CAACACTAGA GCCGCCACCC TCCCCCTCTT CCCACTCTCC TTTTTTCTCC 
TACCAGCCTG CCAGTCTGAC GGTTCCCTCC TCAGGTGGGA CTAGTTCTCC CTCTCCTCAT 
TTTGCTCCAC AGAGGTTGAC GGACCAACCC CCTGTCTCTG TGTCTATGCA GGATGAAGCC 
CAGAGCAGGC CAGAGAACAG GACCAACGCT GTGATGGAGA TGAGCAGTGT AGGGAAGAAG 
GTCCCTGTTA AAATCGTCCA TGCTGAGAGC ACCACAGAGA GGGAGAGCCG CCAGTACCTG 
CTGCACAGCG AGAAAAATAG GGCCCCTGGA GTTTCCGAGG GGCCCGACCT TCCCCCGCCC 
CTACCGACCA GCCTGCCCTC CCCTGAGCCG CAGCCCTACT CCCTGTTCCG TGCCTACACC 
CCCTACACAC GCCATGGGCC CCAGAGTCCC CCCAGAGACC CAACCCTCAC TGTAGCCCCA 
GAAGAGGCCC TGTCCCCTGG GCAGTCTCAG ACAAACGGCC CCTCCGGTAC CTCCACCATG 
GGTCCCCAGC AGCCTCAGAA GAGTAACTCA GACGAGGATG TGAAGAGAGA GGAGCTGGCC 
AGAGACATCA TGGACAAGGA TAAGTCCCTG GTAGACATCT TGGACCAGAG TAAGATGAAG 
ACCACCATGG ATCTGATGGA GGGGATCTTC CCCCAGGGGG AGCAGATCTT GGATGGGGGG 
CACCAGAGGA GGAAAGTCTC CCCCAAACAG GGATTGCCGC CCAGGGGAAT GGATGACCGG 
AGAGAGGAGG AGGGCATGTC TGCTGCCACA GGGTCCCTGG TGACCAGCTC CTCTTACTAC 
AGCACATCTG CCCCCAAGGC TGAGCTGCTC AACAAGATGA AGGACATGCA GGAGGACGAG 
CTGGAGGAGC TGGAGCAAGA CTCGGAGGAC GAACTGGACA TCAACCTGGC CAGCAAGAAG 
CAAGAGCTGA TTGACAGCCT GGGTAAGAAG CTGCAGGTGC TACGCGAGGC CAGGGAGAGC 
CTTCAAGAGG ATGTCCATGA CAACAACTCT CTGGGGGACG AGGTGGAAGC CGTGGTGCAG 
AAGGTCTGCA AGCCCAACGA GCTGGACAAG TTCAGGATGT TCGTGGGAGA TCTGGACAAG 
GTGGTCAGTT TGCTGCTGTC CCTGTCTGGC CGACTGGCCA GAGTGGAGAA CGCTCTCAAC 
AGTCTGGAGG AAGACACTAC ACCAGAAGAG AAGCGTACCC TGTCAGAGAA GAGGAAGTTG 
TTGATCAGAC AACACGAAGA CGCCAAAGAG CTCAAGGAGA ACCTTGACCG GCGGGAGCGC 
CTGGTTTACA GCATCATGGC TGCTCACCTC AGCCACGAGA GCCTGGCAGA CTACCAGCAC 
TTTGTCAAGA TGAAGTCAGC CCTCATCATC GAGCAGCGCA AGCTGGAAGA CAAGATAAAA 
CTGGGAGAGG AGCAGCTGAA ATGTCTGCTG GATAGTTTAT TGTTGGAGCA AAGGCTGCTG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021427059.1
CDS	589..5814
Translation	MDVVDYRSELRMSAREVKAFLGADIFTAVGDRNKDDVEYRFVDVMLFGGAPWGFTLRGGLEHREPLLITKVEEGSKAATVSLQVGDEIVNINTVPISGSRQEAVCLVKSSHKTLALVVRRRNEPVSRPHSWHSSKFTEDHPEPTESHNEPPPAPPPVWQVKHEVSASTKDLSSCGDHDSNLRQLSSQFSSVGNMERVDRPSHPFQPGCLSPSRYHRSAEPLSGGGGSGGKRESAFSCLSSTSPPPEPALANTDTAATESSMFHKGVQTQTSEDGRPGEQRHSRYLQRPQGDGGSESPRTVPEEQPGSRYSSSGSGRSHIGPVWHVPERRRVAAPPSPPLRSDSFAVTKVYPAYTEGPGAPPHGQMKAQDRLAEAPENSSYRGRGNHISHDENGVDPRCSYNPPPCKKDFFHPNIAAGAPDYNHNQLSNPNKLSSVSSQDVRQSQSPFTCLPNHQRQYSDESTFYLQTRSAPHPPKPQSVGSYYHSLQELPTNCTNSRNHVRSSTTSLSTSTIDQNYDCGGHIRYYCITTKQPAQPETHVRQSKSEVWRSDMELAKGSNDRGSTSSSHKTNKVKYPPNPQPAYTNSKERNGNFQPANVLLYGHTTSNSLSSSAKPTTEENERKSEGQRPTEAQLRSSYSPSPPKDHKVGPLREDPWVSQENHKISSQKTPMLHSLAQESRSLAQEIRSPMLHSLAQESKSLVENNHPATAQPPPSNGGGEANHALQEALTDNTATGKLARRSDRYATTLRNEIQLKRAQLQKSRSAATLTCPSETEEPEEEETDPGGWKSTSSTSSDGSFSTSYKDHLKEAQARVLQATSFRRRDLEPPGSGSEAPLTKPNSHIVSRIGSRKRFPLNKRVHSFSEPDKINKLGVEGEGEHPVGTARSFIDRRKFFEMAAKPSFCRPISTIHKSGQQSSSTTSGTLERGEGKARGRAHSGETEEGWRPGLANETSSDHHPDPLSPAGRQALLEQQRLGTFAEYQVTWNMQRKASDTKPQGRYHSADNILDQGTEETPVCVHERSRSSPSQDFHTQKIPMPWRETVENPYLDHRRDQQGGSYTARGQSESRGQPAQLHHFPQPPQPSIPRLTDTEPHSSRDVATPTPLPLPSEHRYKRDSANPAHNLPALPPYPSNHTHSSVSEQPLPPPTPAPKPQNTGLIIMPHWPLLGLETSTTSSSYGLSPQEFLPGPCTHQAEPSSSSSCSSHGTELDPAPNQACSLGSTQLPSPSPRRTTGLGTEEGAADSTLEPPPSPSSHSPFFSYQPASLTVPSSGGTSSPSPHFAPQRLTDQPPVSVSMQDEAQSRPENRTNAVMEMSSVGKKVPVKIVHAESTTERESRQYLLHSEKNRAPGVSEGPDLPPPLPTSLPSPEPQPYSLFRAYTPYTRHGPQSPPRDPTLTVAPEEALSPGQSQTNGPSGTSTMGPQQPQKSNSDEDVKREELARDIMDKDKSLVDILDQSKMKTTMDLMEGIFPQGEQILDGGHQRRKVSPKQGLPPRGMDDRREEEGMSAATGSLVTSSSYYSTSAPKAELLNKMKDMQEDELEELEQDSEDELDINLASKKQELIDSLGKKLQVLREARESLQEDVHDNNSLGDEVEAVVQKVCKPNELDKFRMFVGDLDKVVSLLLSLSGRLARVENALNSLEEDTTPEEKRTLSEKRKLLIRQHEDAKELKENLDRRERLVYSIMAAHLSHESLADYQHFVKMKSALIIEQRKLEDKIKLGEEQLKCLLDSLLLEQRLLF

Target ORF information:

RefSeq Version	XM_021571384.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021571384.1

ORF Insert Sequence:

ATGGACGTCG TCGACTACAG AAGCGAGTTA AGGATGTCAG CCAGAGAAGT CAAAGCCTTC 
CTAGGCGCGG ACATATTCAC GGCTGTCGGG GACAGGAATA AGGATGATGT TGAATATAGA 
TTTGTGGATG TCATGCTGTT TGGTGGGGCC CCTTGGGGGT TCACTCTGAG AGGGGGCCTG 
GAGCACCGAG AACCACTGCT CATAACCAAG GTGGAGGAGG GCAGCAAGGC GGCGACAGTC 
AGCCTGCAGG TGGGAGATGA GATCGTCAAC ATCAACACAG TTCCCATCAG TGGATCCAGG 
CAGGAGGCCG TCTGTCTGGT CAAGAGCTCC CACAAGACCC TCGCCCTGGT TGTCCGAAGG 
AGGAATGAAC CAGTCTCCCG TCCCCATTCA TGGCACTCCT CCAAGTTTAC AGAAGACCAC 
CCCGAACCCA CAGAGAGTCA CAACGAGCCT CCACCTGCAC CCCCACCTGT GTGGCAGGTC 
AAACATGAAG TCAGTGCATC CACAAAAGAC CTTTCCAGCT GCGGGGACCA CGACTCGAAT 
CTACGCCAGT TATCCAGCCA GTTCAGCTCC GTGGGGAATA TGGAGAGAGT AGATCGCCCC 
TCACACCCCT TCCAACCAGG ATGCCTCTCC CCCAGCAGGT ACCACCGCAG CGCTGAGCCT 
CTCTCTGGGG GTGGAGGGTC TGGTGGGAAG AGAGAATCGG CCTTCAGTTG CCTCTCCTCC 
ACCAGCCCTC CCCCGGAGCC TGCTCTGGCC AACACTGACA CTGCTGCAAC TGAGAGCAGC 
ATGTTCCATA AGGGGGTCCA GACTCAGACC AGTGAGGATG GAAGGCCGGG TGAGCAGCGC 
CACAGCCGGT ACCTCCAGCG GCCCCAAGGG GACGGAGGCT CGGAGAGCCC CAGGACAGTC 
CCAGAGGAGC AGCCTGGCTC CCGCTACTCC AGTTCAGGCT CTGGCAGATC GCACATAGGC 
CCTGTGTGGC ATGTCCCAGA GAGGAGGAGG GTAGCAGCGC CCCCCTCTCC TCCCCTGCGC 
AGTGACAGCT TTGCTGTCAC CAAGGTGTAC CCTGCCTACA CAGAGGGGCC TGGAGCTCCA 
CCACACGGGC AGATGAAGGC CCAGGACAGG TTGGCAGAGG CCCCAGAGAA CAGCAGCTAC 
AGAGGCCGGG GCAACCATAT CTCTCACGAT GAGAACGGTG TAGACCCTAG GTGCAGTTAC 
AACCCTCCAC CTTGCAAGAA AGACTTCTTC CACCCAAACA TAGCAGCAGG AGCACCTGAC 
TACAACCACA ACCAGCTCAG CAACCCAAAC AAACTGTCCT CTGTGTCTAG CCAGGACGTG 
AGACAGAGTC AGTCTCCCTT CACTTGCCTG CCCAACCACC AGCGGCAGTA TAGCGACGAA 
AGCACTTTCT ACCTGCAGAC CAGATCCGCC CCACATCCAC CGAAGCCGCA GAGCGTCGGT 
AGCTACTACC ACAGCCTCCA GGAGCTCCCT ACCAACTGCA CTAACAGCAG GAACCACGTG 
AGGTCCTCCA CCACGTCCCT GTCCACCTCG ACCATCGACC AGAACTATGA CTGCGGAGGA 
CACATCCGGT ACTACTGCAT CACAACCAAG CAGCCAGCCC AGCCAGAGAC TCATGTTAGA 
CAGAGCAAAT CAGAGGTCTG GAGATCTGAC ATGGAGCTAG CTAAGGGCTC CAACGACAGG 
GGCTCTACAA GTTCCTCCCA CAAGACCAAC AAGGTCAAGT ATCCTCCTAA TCCTCAGCCT 
GCTTACACCA ACAGCAAGGA GCGGAACGGA AATTTCCAAC CGGCCAATGT TCTGCTTTAC 
GGCCACACCA CCTCTAATTC CCTATCCTCA TCTGCTAAAC CTACCACTGA GGAGAATGAG 
AGGAAGAGTG AGGGCCAGAG ACCTACAGAG GCCCAGCTTA GAAGTTCTTA CTCTCCCAGT 
CCGCCCAAGG ACCACAAGGT GGGACCACTG AGAGAAGACC CTTGGGTCTC TCAGGAGAAC 
CATAAGATCT CTTCTCAAAA GACACCCATG CTCCACAGTC TGGCTCAGGA GAGTAGAAGC 
CTAGCCCAGG AGATTAGGAG CCCAATGCTT CATTCTCTAG CCCAGGAGAG TAAGAGCCTG 
GTGGAGAACA ATCACCCTGC TACGGCACAA CCACCACCAT CCAACGGCGG GGGAGAAGCC 
AACCACGCCC TACAGGAGGC TTTAACGGAC AACACGGCAA CGGGCAAACT GGCGAGACGC 
AGCGACCGAT ACGCCACCAC CCTCCGCAAC GAGATCCAGC TAAAGAGGGC CCAGCTGCAG 
AAAAGCCGGA GTGCTGCCAC CCTGACCTGC CCCAGCGAGA CGGAGGAGCC AGAGGAAGAG 
GAGACAGACC CGGGGGGGTG GAAGTCCACC TCCTCCACCT CCTCTGATGG CTCCTTTTCC 
ACGTCCTACA AGGACCACCT CAAAGAGGCG CAGGCGAGGG TCCTCCAGGC CACGTCCTTT 
AGGAGGAGAG ACCTAGAACC TCCCGGGTCA GGGTCGGAGG CTCCGTTAAC CAAACCCAAC 
TCACACATAG TCTCCCGCAT TGGCAGCCGC AAGCGTTTCC CTCTGAACAA GAGGGTCCAC 
TCGTTCTCCG AGCCAGACAA GATCAACAAG CTCGGAGTGG AGGGTGAGGG AGAACATCCT 
GTTGGAACAG CACGGTCATT TATAGACCGG CGGAAGTTCT TTGAAATGGC TGCTAAACCT 
TCCTTCTGCA GACCCATCTC AACCATCCAC AAGTCAGGCC AGCAGAGCTC CAGCACCACC 
TCTGGCACTT TGGAGCGCGG AGAGGGCAAG GCCAGAGGGA GAGCCCACTC AGGCGAAACT 
GAGGAGGGCT GGAGGCCAGG CCTTGCCAAT GAGACTTCCA GTGACCACCA CCCAGACCCC 
CTCAGCCCCG CAGGCAGACA GGCCCTACTG GAGCAGCAGA GGCTGGGGAC CTTCGCTGAG 
TACCAGGTCA CCTGGAACAT GCAGAGGAAG GCTTCAGACA CAAAGCCCCA GGGGAGGTAC 
CACTCTGCTG ACAACATCCT GGACCAGGGT ACAGAGGAGA CGCCTGTCTG TGTCCATGAG 
AGGTCCAGAT CGTCTCCCTC ACAAGACTTC CACACACAGA AGATCCCTAT GCCATGGAGA 
GAGACTGTTG AAAACCCGTA TCTGGATCAC AGACGGGACC AGCAAGGAGG CAGTTACACC 
GCCAGAGGGC AGAGCGAGAG CAGAGGGCAG CCAGCCCAGC TCCACCACTT CCCACAGCCT 
CCACAACCGT CTATTCCAAG ACTGACCGAC ACAGAGCCAC ACAGCAGCAG AGACGTGGCC 
ACCCCCACCC CCCTCCCTCT CCCCTCCGAA CACAGATACA AACGTGACTC TGCGAACCCC 
GCCCACAACC TCCCCGCTCT CCCCCCGTAC CCCTCCAATC ACACCCACAG CTCTGTGTCT 
GAGCAACCAC TACCACCTCC AACGCCCGCT CCCAAGCCCC AGAATACAGG CCTCATCATC 
ATGCCACATT GGCCTCTCCT AGGCCTGGAA ACCTCAACCA CATCCTCCTC CTACGGACTG 
TCTCCCCAGG AATTCCTGCC TGGCCCTTGC ACCCATCAGG CTGAACCATC ATCCAGCAGC 
AGCTGCTCCT CCCATGGCAC AGAGCTGGAT CCTGCCCCAA ACCAAGCCTG CTCCTTGGGC 
TCCACCCAGC TCCCCTCTCC CTCCCCTCGG AGAACCACTG GACTGGGCAC GGAGGAGGGA 
GCAGCTGATT CAACACTAGA GCCGCCACCC TCCCCCTCTT CCCACTCTCC TTTTTTCTCC 
TACCAGCCTG CCAGTCTGAC GGTTCCCTCC TCAGGTGGGA CTAGTTCTCC CTCTCCTCAT 
TTTGCTCCAC AGAGGTTGAC GGACCAACCC CCTGTCTCTG TGTCTATGCA GGATGAAGCC 
CAGAGCAGGC CAGAGAACAG GACCAACGCT GTGATGGAGA TGAGCAGTGT AGGGAAGAAG 
GTCCCTGTTA AAATCGTCCA TGCTGAGAGC ACCACAGAGA GGGAGAGCCG CCAGTACCTG 
CTGCACAGCG AGAAAAATAG GGCCCCTGGA GTTTCCGAGG GGCCCGACCT TCCCCCGCCC 
CTACCGACCA GCCTGCCCTC CCCTGAGCCG CAGCCCTACT CCCTGTTCCG TGCCTACACC 
CCCTACACAC GCCATGGGCC CCAGAGTCCC CCCAGAGACC CAACCCTCAC TGTAGCCCCA 
GAAGAGGCCC TGTCCCCTGG GCAGTCTCAG ACAAACGGCC CCTCCGGTAC CTCCACCATG 
GGTCCCCAGC AGCCTCAGAA GAGTAACTCA GACGAGGATG TGAAGAGAGA GGAGCTGGCC 
AGAGACATCA TGGACAAGGA TAAGTCCCTG GTAGACATCT TGGACCAGAG TAAGATGAAG 
ACCACCATGG ATCTGATGGA GGGGATCTTC CCCCAGGGGG AGCAGATCTT GGATGGGGGG 
CACCAGAGGA GGAAAGTCTC CCCCAAACAG GGATTGCCGC CCAGGGGAAT GGATGACCGG 
AGAGAGGAGG AGGGCATGTC TGCTGCCACA GGGTCCCTGG TGACCAGCTC CTCTTACTAC 
AGCACATCTG CCCCCAAGGC TGAGCTGCTC AACAAGATGA AGGACATGCA GGAGGACGAG 
CTGGAGGAGC TGGAGCAAGA CTCGGAGGAC GAACTGGACA TCAACCTGGC CAGCAAGAAG 
CAAGAGCTGA TTGACAGCCT GGGTAAGAAG CTGCAGGTGC TACGCGAGGC CAGGGAGAGC 
CTTCAAGAGG ATGTCCATGA CAACAACTCT CTGGGGGACG AGGTGGAAGC CGTGGTGCAG 
AAGGTCTGCA AGCCCAACGA GCTGGACAAG TTCAGGATGT TCGTGGGAGA TCTGGACAAG 
GTGGTCAGTT TGCTGCTGTC CCTGTCTGGC CGACTGGCCA GAGTGGAGAA CGCTCTCAAC 
AGTCTGGAGG AAGACACTAC ACCAGAAGAG AAGCGTACCC TGTCAGAGAA GAGGAAGTTG 
TTGATCAGAC AACACGAAGA CGCCAAAGAG CTCAAGGAGA ACCTTGACCG GCGGGAGCGC 
CTGGTTTACA GCATCATGGC TGCTCACCTC AGCCACGAGA GCCTGGCAGA CTACCAGCAC 
TTTGTCAAGA TGAAGTCAGC CCTCATCATC GAGCAGCGCA AGCTGGAAGA CAAGATAAAA 
CTGGGAGAGG AGCAGCTGAA ATGTCTGCTG GATAGTTTAT TGTTGGAGCA AAGGCTGCTG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110495881 ( XM_021571385.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021427060.1 Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf19772
Clone ID Related Accession (Same CDS sequence)	XM_021571385.1
Accession Version	XM_021571385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	protein Shroom2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTCG TCGACTACAG AAGCGAGTTA AGGATGTCAG CCAGAGAAGT CAAAGCCTTC 
CTAGGCGCGG ACATATTCAC GGCTGTCGGG GACAGGAATA AGGATGATGT TGAATATAGA 
TTTGTGGATG TCATGCTGTT TGGTGGGGCC CCTTGGGGGT TCACTCTGAG AGGGGGCCTG 
GAGCACCGAG AACCACTGCT CATAACCAAG GTGGAGGAGG GCAGCAAGGC GGCGACAGTC 
AGCCTGCAGG TGGGAGATGA GATCGTCAAC ATCAACACAG TTCCCATCAG TGGATCCAGG 
CAGGAGGCCG TCTGTCTGGT CAAGAGCTCC CACAAGACCC TCGCCCTGGT TGTCCGAAGG 
AGGAATGAAC CAGTCTCCCG TCCCCATTCA TGGCACTCCT CCAAGTTTAC AGAAGACCAC 
CCCGAACCCA CAGAGAGTCA CAACGAGCCT CCACCTGCAC CCCCACCTGT GTGGCAGGTC 
AAACATGAAG TCAGTGCATC CACAAAAGAC CTTTCCAGCT GCGGGGACCA CGACTCGAAT 
CTACGCCAGT TATCCAGCCA GTTCAGCTCC GTGGGGAATA TGGAGAGAGT AGATCGCCCC 
TCACACCCCT TCCAACCAGG ATGCCTCTCC CCCAGCAGGT ACCACCGCAG CGCTGAGCCT 
CTCTCTGGGG GTGGAGGGTC TGGTGGGAAG AGAGAATCGG CCTTCAGTTG CCTCTCCTCC 
ACCAGCCCTC CCCCGGAGCC TGCTCTGGCC AACACTGACA CTGCTGCAAC TGAGAGCAGC 
ATGTTCCATA AGGGGGTCCA GACTCAGACC AGTGAGGATG GAAGGCCGGG TGAGCAGCGC 
CACAGCCGGT ACCTCCAGCG GCCCCAAGGG GACGGAGGCT CGGAGAGCCC CAGGACAGTC 
CCAGAGGAGC AGCCTGGCTC CCGCTACTCC AGTTCAGGCT CTGGCAGATC GCACATAGGC 
CCTGTGTGGC ATGTCCCAGA GAGGAGGAGG GTAGCAGCGC CCCCCTCTCC TCCCCTGCGC 
AGTGACAGCT TTGCTGTCAC CAAGGTGTAC CCTGCCTACA CAGAGGGGCC TGGAGCTCCA 
CCACACGGGC AGATGAAGGC CCAGGACAGG TTGGCAGAGG CCCCAGAGAA CAGCAGCTAC 
AGAGGCCGGG GCAACCATAT CTCTCACGAT GAGAACGGTG TAGACCCTAG GTGCAGTTAC 
AACCCTCCAC CTTGCAAGAA AGACTTCTTC CACCCAAACA TAGCAGCAGG AGCACCTGAC 
TACAACCACA ACCAGCTCAG CAACCCAAAC AAACTGTCCT CTGTGTCTAG CCAGGACGTG 
AGACAGAGTC AGTCTCCCTT CACTTGCCTG CCCAACCACC AGCGGCAGTA TAGCGACGAA 
AGCACTTTCT ACCTGCAGAC CAGATCCGCC CCACATCCAC CGAAGCCGCA GAGCGTCGGT 
AGCTACTACC ACAGCCTCCA GGAGCTCCCT ACCAACTGCA CTAACAGCAG GAACCACGTG 
AGGTCCTCCA CCACGTCCCT GTCCACCTCG ACCATCGACC AGAACTATGA CTGCGGAGGA 
CACATCCGGT ACTACTGCAT CACAACCAAG CAGCCAGCCC AGCCAGAGAC TCATGTTAGA 
CAGAGCAAAT CAGAGGTCTG GAGATCTGAC ATGGAGCTAG CTAAGGGCTC CAACGACAGG 
GGCTCTACAA GTTCCTCCCA CAAGACCAAC AAGGTCAAGT ATCCTCCTAA TCCTCAGCCT 
GCTTACACCA ACAGCAAGGA GCGGAACGGA AATTTCCAAC CGGCCAATGT TCTGCTTTAC 
GGCCACACCA CCTCTAATTC CCTATCCTCA TCTGCTAAAC CTACCACTGA GGAGAATGAG 
AGGAAGAGTG AGGGCCAGAG ACCTACAGAG GCCCAGCTTA GAAGTTCTTA CTCTCCCAGT 
CCGCCCAAGG ACCACAAGGT GGGACCACTG AGAGAAGACC CTTGGGTCTC TCAGGAGAAC 
CATAAGATCT CTTCTCAAAA GACACCCATG CTCCACAGTC TGGCTCAGGA GAGTAGAAGC 
CTAGCCCAGG AGATTAGGAG CCCAATGCTT CATTCTCTAG CCCAGGAGAG TAAGAGCCTG 
GTGGAGAACA ATCACCCTGC TACGGCACAA CCACCACCAT CCAACGGCGG GGGAGAAGCC 
AACCACGCCC TACAGGAGGC TTTAACGGAC AACACGGCAA CGGGCAAACT GGCGAGACGC 
AGCGACCGAT ACGCCACCAC CCTCCGCAAC GAGATCCAGC TAAAGAGGGC CCAGCTGCAG 
AAAAGCCGGA GTGCTGCCAC CCTGACCTGC CCCAGCGAGA CGGAGGAGCC AGAGGAAGAG 
GAGACAGACC CGGGGGGGTG GAAGTCCACC TCCTCCACCT CCTCTGATGG CTCCTTTTCC 
ACGTCCTACA AGGACCACCT CAAAGAGGCG CAGGCGAGGG TCCTCCAGGC CACGTCCTTT 
AGGAGGAGAG ACCTAGAACC TCCCGGGTCA GGGTCGGAGG CTCCGTTAAC CAAACCCAAC 
TCACACATAG TCTCCCGCAT TGGCAGCCGC AAGCGTTTCC CTCTGAACAA GAGGGTCCAC 
TCGTTCTCCG AGCCAGACAA GATCAACAAG CTCGGAGTGG AGGGTGAGGG AGAACATCCT 
GTTGGAACAG CACGGTCATT TATAGACCGG CGGAAGTTCT TTGAAATGGC TGCTAAACCT 
TCCTTCTGCA GACCCATCTC AACCATCCAC AAGTCAGGCC AGCAGAGCTC CAGCACCACC 
TCTGGCACTT TGGAGCGCGG AGAGGGCAAG GCCAGAGGGA GAGCCCACTC AGGCGAAACT 
GAGGAGGGCT GGAGGCCAGG CCTTGCCAAT GAGACTTCCA GTGACCACCA CCCAGACCCC 
CTCAGCCCCG CAGGCAGACA GGCCCTACTG GAGCAGCAGA GGCTGGGGAC CTTCGCTGAG 
TACCAGGTCA CCTGGAACAT GCAGAGGAAG GCTTCAGACA CAAAGCCCCA GGGGAGGTAC 
CACTCTGCTG ACAACATCCT GGACCAGGGT ACAGAGGAGA CGCCTGTCTG TGTCCATGAG 
AGGTCCAGAT CGTCTCCCTC ACAAGACTTC CACACACAGA TCCCTATGCC ATGGAGAGAG 
ACTGTTGAAA ACCCGTATCT GGATCACAGA CGGGACCAGC AAGGAGGCAG TTACACCGCC 
AGAGGGCAGA GCGAGAGCAG AGGGCAGCCA GCCCAGCTCC ACCACTTCCC ACAGCCTCCA 
CAACCGTCTA TTCCAAGACT GACCGACACA GAGCCACACA GCAGCAGAGA CGTGGCCACC 
CCCACCCCCC TCCCTCTCCC CTCCGAACAC AGATACAAAC GTGACTCTGC GAACCCCGCC 
CACAACCTCC CCGCTCTCCC CCCGTACCCC TCCAATCACA CCCACAGCTC TGTGTCTGAG 
CAACCACTAC CACCTCCAAC GCCCGCTCCC AAGCCCCAGA ATACAGGCCT CATCATCATG 
CCACATTGGC CTCTCCTAGG CCTGGAAACC TCAACCACAT CCTCCTCCTA CGGACTGTCT 
CCCCAGGAAT TCCTGCCTGG CCCTTGCACC CATCAGGCTG AACCATCATC CAGCAGCAGC 
TGCTCCTCCC ATGGCACAGA GCTGGATCCT GCCCCAAACC AAGCCTGCTC CTTGGGCTCC 
ACCCAGCTCC CCTCTCCCTC CCCTCGGAGA ACCACTGGAC TGGGCACGGA GGAGGGAGCA 
GCTGATTCAA CACTAGAGCC GCCACCCTCC CCCTCTTCCC ACTCTCCTTT TTTCTCCTAC 
CAGCCTGCCA GTCTGACGGT TCCCTCCTCA GGTGGGACTA GTTCTCCCTC TCCTCATTTT 
GCTCCACAGA GGTTGACGGA CCAACCCCCT GTCTCTGTGT CTATGCAGGA TGAAGCCCAG 
AGCAGGCCAG AGAACAGGAC CAACGCTGTG ATGGAGATGA GCAGTGTAGG GAAGAAGGTC 
CCTGTTAAAA TCGTCCATGC TGAGAGCACC ACAGAGAGGG AGAGCCGCCA GTACCTGCTG 
CACAGCGAGA AAAATAGGGC CCCTGGAGTT TCCGAGGGGC CCGACCTTCC CCCGCCCCTA 
CCGACCAGCC TGCCCTCCCC TGAGCCGCAG CCCTACTCCC TGTTCCGTGC CTACACCCCC 
TACACACGCC ATGGGCCCCA GAGTCCCCCC AGAGACCCAA CCCTCACTGT AGCCCCAGAA 
GAGGCCCTGT CCCCTGGGCA GTCTCAGACA AACGGCCCCT CCGGTACCTC CACCATGGGT 
CCCCAGCAGC CTCAGAAGAG TAACTCAGAC GAGGATGTGA AGAGAGAGGA GCTGGCCAGA 
GACATCATGG ACAAGGATAA GTCCCTGGTA GACATCTTGG ACCAGAGTAA GATGAAGACC 
ACCATGGATC TGATGGAGGG GATCTTCCCC CAGGGGGAGC AGATCTTGGA TGGGGGGCAC 
CAGAGGAGGA AAGTCTCCCC CAAACAGGGA TTGCCGCCCA GGGGAATGGA TGACCGGAGA 
GAGGAGGAGG GCATGTCTGC TGCCACAGGG TCCCTGGTGA CCAGCTCCTC TTACTACAGC 
ACATCTGCCC CCAAGGCTGA GCTGCTCAAC AAGATGAAGG ACATGCAGGA GGACGAGCTG 
GAGGAGCTGG AGCAAGACTC GGAGGACGAA CTGGACATCA ACCTGGCCAG CAAGAAGCAA 
GAGCTGATTG ACAGCCTGGG TAAGAAGCTG CAGGTGCTAC GCGAGGCCAG GGAGAGCCTT 
CAAGAGGATG TCCATGACAA CAACTCTCTG GGGGACGAGG TGGAAGCCGT GGTGCAGAAG 
GTCTGCAAGC CCAACGAGCT GGACAAGTTC AGGATGTTCG TGGGAGATCT GGACAAGGTG 
GTCAGTTTGC TGCTGTCCCT GTCTGGCCGA CTGGCCAGAG TGGAGAACGC TCTCAACAGT 
CTGGAGGAAG ACACTACACC AGAAGAGAAG CGTACCCTGT CAGAGAAGAG GAAGTTGTTG 
ATCAGACAAC ACGAAGACGC CAAAGAGCTC AAGGAGAACC TTGACCGGCG GGAGCGCCTG 
GTTTACAGCA TCATGGCTGC TCACCTCAGC CACGAGAGCC TGGCAGACTA CCAGCACTTT 
GTCAAGATGA AGTCAGCCCT CATCATCGAG CAGCGCAAGC TGGAAGACAA GATAAAACTG 
GGAGAGGAGC AGCTGAAATG TCTGCTGGAT AGTTTATTGT TGGAGCAAAG GCTGCTGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021427060.1
CDS	589..5811
Translation	MDVVDYRSELRMSAREVKAFLGADIFTAVGDRNKDDVEYRFVDVMLFGGAPWGFTLRGGLEHREPLLITKVEEGSKAATVSLQVGDEIVNINTVPISGSRQEAVCLVKSSHKTLALVVRRRNEPVSRPHSWHSSKFTEDHPEPTESHNEPPPAPPPVWQVKHEVSASTKDLSSCGDHDSNLRQLSSQFSSVGNMERVDRPSHPFQPGCLSPSRYHRSAEPLSGGGGSGGKRESAFSCLSSTSPPPEPALANTDTAATESSMFHKGVQTQTSEDGRPGEQRHSRYLQRPQGDGGSESPRTVPEEQPGSRYSSSGSGRSHIGPVWHVPERRRVAAPPSPPLRSDSFAVTKVYPAYTEGPGAPPHGQMKAQDRLAEAPENSSYRGRGNHISHDENGVDPRCSYNPPPCKKDFFHPNIAAGAPDYNHNQLSNPNKLSSVSSQDVRQSQSPFTCLPNHQRQYSDESTFYLQTRSAPHPPKPQSVGSYYHSLQELPTNCTNSRNHVRSSTTSLSTSTIDQNYDCGGHIRYYCITTKQPAQPETHVRQSKSEVWRSDMELAKGSNDRGSTSSSHKTNKVKYPPNPQPAYTNSKERNGNFQPANVLLYGHTTSNSLSSSAKPTTEENERKSEGQRPTEAQLRSSYSPSPPKDHKVGPLREDPWVSQENHKISSQKTPMLHSLAQESRSLAQEIRSPMLHSLAQESKSLVENNHPATAQPPPSNGGGEANHALQEALTDNTATGKLARRSDRYATTLRNEIQLKRAQLQKSRSAATLTCPSETEEPEEEETDPGGWKSTSSTSSDGSFSTSYKDHLKEAQARVLQATSFRRRDLEPPGSGSEAPLTKPNSHIVSRIGSRKRFPLNKRVHSFSEPDKINKLGVEGEGEHPVGTARSFIDRRKFFEMAAKPSFCRPISTIHKSGQQSSSTTSGTLERGEGKARGRAHSGETEEGWRPGLANETSSDHHPDPLSPAGRQALLEQQRLGTFAEYQVTWNMQRKASDTKPQGRYHSADNILDQGTEETPVCVHERSRSSPSQDFHTQIPMPWRETVENPYLDHRRDQQGGSYTARGQSESRGQPAQLHHFPQPPQPSIPRLTDTEPHSSRDVATPTPLPLPSEHRYKRDSANPAHNLPALPPYPSNHTHSSVSEQPLPPPTPAPKPQNTGLIIMPHWPLLGLETSTTSSSYGLSPQEFLPGPCTHQAEPSSSSSCSSHGTELDPAPNQACSLGSTQLPSPSPRRTTGLGTEEGAADSTLEPPPSPSSHSPFFSYQPASLTVPSSGGTSSPSPHFAPQRLTDQPPVSVSMQDEAQSRPENRTNAVMEMSSVGKKVPVKIVHAESTTERESRQYLLHSEKNRAPGVSEGPDLPPPLPTSLPSPEPQPYSLFRAYTPYTRHGPQSPPRDPTLTVAPEEALSPGQSQTNGPSGTSTMGPQQPQKSNSDEDVKREELARDIMDKDKSLVDILDQSKMKTTMDLMEGIFPQGEQILDGGHQRRKVSPKQGLPPRGMDDRREEEGMSAATGSLVTSSSYYSTSAPKAELLNKMKDMQEDELEELEQDSEDELDINLASKKQELIDSLGKKLQVLREARESLQEDVHDNNSLGDEVEAVVQKVCKPNELDKFRMFVGDLDKVVSLLLSLSGRLARVENALNSLEEDTTPEEKRTLSEKRKLLIRQHEDAKELKENLDRRERLVYSIMAAHLSHESLADYQHFVKMKSALIIEQRKLEDKIKLGEEQLKCLLDSLLLEQRLLF

Target ORF information:

RefSeq Version	XM_021571385.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021571385.1

ORF Insert Sequence:

ATGGACGTCG TCGACTACAG AAGCGAGTTA AGGATGTCAG CCAGAGAAGT CAAAGCCTTC 
CTAGGCGCGG ACATATTCAC GGCTGTCGGG GACAGGAATA AGGATGATGT TGAATATAGA 
TTTGTGGATG TCATGCTGTT TGGTGGGGCC CCTTGGGGGT TCACTCTGAG AGGGGGCCTG 
GAGCACCGAG AACCACTGCT CATAACCAAG GTGGAGGAGG GCAGCAAGGC GGCGACAGTC 
AGCCTGCAGG TGGGAGATGA GATCGTCAAC ATCAACACAG TTCCCATCAG TGGATCCAGG 
CAGGAGGCCG TCTGTCTGGT CAAGAGCTCC CACAAGACCC TCGCCCTGGT TGTCCGAAGG 
AGGAATGAAC CAGTCTCCCG TCCCCATTCA TGGCACTCCT CCAAGTTTAC AGAAGACCAC 
CCCGAACCCA CAGAGAGTCA CAACGAGCCT CCACCTGCAC CCCCACCTGT GTGGCAGGTC 
AAACATGAAG TCAGTGCATC CACAAAAGAC CTTTCCAGCT GCGGGGACCA CGACTCGAAT 
CTACGCCAGT TATCCAGCCA GTTCAGCTCC GTGGGGAATA TGGAGAGAGT AGATCGCCCC 
TCACACCCCT TCCAACCAGG ATGCCTCTCC CCCAGCAGGT ACCACCGCAG CGCTGAGCCT 
CTCTCTGGGG GTGGAGGGTC TGGTGGGAAG AGAGAATCGG CCTTCAGTTG CCTCTCCTCC 
ACCAGCCCTC CCCCGGAGCC TGCTCTGGCC AACACTGACA CTGCTGCAAC TGAGAGCAGC 
ATGTTCCATA AGGGGGTCCA GACTCAGACC AGTGAGGATG GAAGGCCGGG TGAGCAGCGC 
CACAGCCGGT ACCTCCAGCG GCCCCAAGGG GACGGAGGCT CGGAGAGCCC CAGGACAGTC 
CCAGAGGAGC AGCCTGGCTC CCGCTACTCC AGTTCAGGCT CTGGCAGATC GCACATAGGC 
CCTGTGTGGC ATGTCCCAGA GAGGAGGAGG GTAGCAGCGC CCCCCTCTCC TCCCCTGCGC 
AGTGACAGCT TTGCTGTCAC CAAGGTGTAC CCTGCCTACA CAGAGGGGCC TGGAGCTCCA 
CCACACGGGC AGATGAAGGC CCAGGACAGG TTGGCAGAGG CCCCAGAGAA CAGCAGCTAC 
AGAGGCCGGG GCAACCATAT CTCTCACGAT GAGAACGGTG TAGACCCTAG GTGCAGTTAC 
AACCCTCCAC CTTGCAAGAA AGACTTCTTC CACCCAAACA TAGCAGCAGG AGCACCTGAC 
TACAACCACA ACCAGCTCAG CAACCCAAAC AAACTGTCCT CTGTGTCTAG CCAGGACGTG 
AGACAGAGTC AGTCTCCCTT CACTTGCCTG CCCAACCACC AGCGGCAGTA TAGCGACGAA 
AGCACTTTCT ACCTGCAGAC CAGATCCGCC CCACATCCAC CGAAGCCGCA GAGCGTCGGT 
AGCTACTACC ACAGCCTCCA GGAGCTCCCT ACCAACTGCA CTAACAGCAG GAACCACGTG 
AGGTCCTCCA CCACGTCCCT GTCCACCTCG ACCATCGACC AGAACTATGA CTGCGGAGGA 
CACATCCGGT ACTACTGCAT CACAACCAAG CAGCCAGCCC AGCCAGAGAC TCATGTTAGA 
CAGAGCAAAT CAGAGGTCTG GAGATCTGAC ATGGAGCTAG CTAAGGGCTC CAACGACAGG 
GGCTCTACAA GTTCCTCCCA CAAGACCAAC AAGGTCAAGT ATCCTCCTAA TCCTCAGCCT 
GCTTACACCA ACAGCAAGGA GCGGAACGGA AATTTCCAAC CGGCCAATGT TCTGCTTTAC 
GGCCACACCA CCTCTAATTC CCTATCCTCA TCTGCTAAAC CTACCACTGA GGAGAATGAG 
AGGAAGAGTG AGGGCCAGAG ACCTACAGAG GCCCAGCTTA GAAGTTCTTA CTCTCCCAGT 
CCGCCCAAGG ACCACAAGGT GGGACCACTG AGAGAAGACC CTTGGGTCTC TCAGGAGAAC 
CATAAGATCT CTTCTCAAAA GACACCCATG CTCCACAGTC TGGCTCAGGA GAGTAGAAGC 
CTAGCCCAGG AGATTAGGAG CCCAATGCTT CATTCTCTAG CCCAGGAGAG TAAGAGCCTG 
GTGGAGAACA ATCACCCTGC TACGGCACAA CCACCACCAT CCAACGGCGG GGGAGAAGCC 
AACCACGCCC TACAGGAGGC TTTAACGGAC AACACGGCAA CGGGCAAACT GGCGAGACGC 
AGCGACCGAT ACGCCACCAC CCTCCGCAAC GAGATCCAGC TAAAGAGGGC CCAGCTGCAG 
AAAAGCCGGA GTGCTGCCAC CCTGACCTGC CCCAGCGAGA CGGAGGAGCC AGAGGAAGAG 
GAGACAGACC CGGGGGGGTG GAAGTCCACC TCCTCCACCT CCTCTGATGG CTCCTTTTCC 
ACGTCCTACA AGGACCACCT CAAAGAGGCG CAGGCGAGGG TCCTCCAGGC CACGTCCTTT 
AGGAGGAGAG ACCTAGAACC TCCCGGGTCA GGGTCGGAGG CTCCGTTAAC CAAACCCAAC 
TCACACATAG TCTCCCGCAT TGGCAGCCGC AAGCGTTTCC CTCTGAACAA GAGGGTCCAC 
TCGTTCTCCG AGCCAGACAA GATCAACAAG CTCGGAGTGG AGGGTGAGGG AGAACATCCT 
GTTGGAACAG CACGGTCATT TATAGACCGG CGGAAGTTCT TTGAAATGGC TGCTAAACCT 
TCCTTCTGCA GACCCATCTC AACCATCCAC AAGTCAGGCC AGCAGAGCTC CAGCACCACC 
TCTGGCACTT TGGAGCGCGG AGAGGGCAAG GCCAGAGGGA GAGCCCACTC AGGCGAAACT 
GAGGAGGGCT GGAGGCCAGG CCTTGCCAAT GAGACTTCCA GTGACCACCA CCCAGACCCC 
CTCAGCCCCG CAGGCAGACA GGCCCTACTG GAGCAGCAGA GGCTGGGGAC CTTCGCTGAG 
TACCAGGTCA CCTGGAACAT GCAGAGGAAG GCTTCAGACA CAAAGCCCCA GGGGAGGTAC 
CACTCTGCTG ACAACATCCT GGACCAGGGT ACAGAGGAGA CGCCTGTCTG TGTCCATGAG 
AGGTCCAGAT CGTCTCCCTC ACAAGACTTC CACACACAGA TCCCTATGCC ATGGAGAGAG 
ACTGTTGAAA ACCCGTATCT GGATCACAGA CGGGACCAGC AAGGAGGCAG TTACACCGCC 
AGAGGGCAGA GCGAGAGCAG AGGGCAGCCA GCCCAGCTCC ACCACTTCCC ACAGCCTCCA 
CAACCGTCTA TTCCAAGACT GACCGACACA GAGCCACACA GCAGCAGAGA CGTGGCCACC 
CCCACCCCCC TCCCTCTCCC CTCCGAACAC AGATACAAAC GTGACTCTGC GAACCCCGCC 
CACAACCTCC CCGCTCTCCC CCCGTACCCC TCCAATCACA CCCACAGCTC TGTGTCTGAG 
CAACCACTAC CACCTCCAAC GCCCGCTCCC AAGCCCCAGA ATACAGGCCT CATCATCATG 
CCACATTGGC CTCTCCTAGG CCTGGAAACC TCAACCACAT CCTCCTCCTA CGGACTGTCT 
CCCCAGGAAT TCCTGCCTGG CCCTTGCACC CATCAGGCTG AACCATCATC CAGCAGCAGC 
TGCTCCTCCC ATGGCACAGA GCTGGATCCT GCCCCAAACC AAGCCTGCTC CTTGGGCTCC 
ACCCAGCTCC CCTCTCCCTC CCCTCGGAGA ACCACTGGAC TGGGCACGGA GGAGGGAGCA 
GCTGATTCAA CACTAGAGCC GCCACCCTCC CCCTCTTCCC ACTCTCCTTT TTTCTCCTAC 
CAGCCTGCCA GTCTGACGGT TCCCTCCTCA GGTGGGACTA GTTCTCCCTC TCCTCATTTT 
GCTCCACAGA GGTTGACGGA CCAACCCCCT GTCTCTGTGT CTATGCAGGA TGAAGCCCAG 
AGCAGGCCAG AGAACAGGAC CAACGCTGTG ATGGAGATGA GCAGTGTAGG GAAGAAGGTC 
CCTGTTAAAA TCGTCCATGC TGAGAGCACC ACAGAGAGGG AGAGCCGCCA GTACCTGCTG 
CACAGCGAGA AAAATAGGGC CCCTGGAGTT TCCGAGGGGC CCGACCTTCC CCCGCCCCTA 
CCGACCAGCC TGCCCTCCCC TGAGCCGCAG CCCTACTCCC TGTTCCGTGC CTACACCCCC 
TACACACGCC ATGGGCCCCA GAGTCCCCCC AGAGACCCAA CCCTCACTGT AGCCCCAGAA 
GAGGCCCTGT CCCCTGGGCA GTCTCAGACA AACGGCCCCT CCGGTACCTC CACCATGGGT 
CCCCAGCAGC CTCAGAAGAG TAACTCAGAC GAGGATGTGA AGAGAGAGGA GCTGGCCAGA 
GACATCATGG ACAAGGATAA GTCCCTGGTA GACATCTTGG ACCAGAGTAA GATGAAGACC 
ACCATGGATC TGATGGAGGG GATCTTCCCC CAGGGGGAGC AGATCTTGGA TGGGGGGCAC 
CAGAGGAGGA AAGTCTCCCC CAAACAGGGA TTGCCGCCCA GGGGAATGGA TGACCGGAGA 
GAGGAGGAGG GCATGTCTGC TGCCACAGGG TCCCTGGTGA CCAGCTCCTC TTACTACAGC 
ACATCTGCCC CCAAGGCTGA GCTGCTCAAC AAGATGAAGG ACATGCAGGA GGACGAGCTG 
GAGGAGCTGG AGCAAGACTC GGAGGACGAA CTGGACATCA ACCTGGCCAG CAAGAAGCAA 
GAGCTGATTG ACAGCCTGGG TAAGAAGCTG CAGGTGCTAC GCGAGGCCAG GGAGAGCCTT 
CAAGAGGATG TCCATGACAA CAACTCTCTG GGGGACGAGG TGGAAGCCGT GGTGCAGAAG 
GTCTGCAAGC CCAACGAGCT GGACAAGTTC AGGATGTTCG TGGGAGATCT GGACAAGGTG 
GTCAGTTTGC TGCTGTCCCT GTCTGGCCGA CTGGCCAGAG TGGAGAACGC TCTCAACAGT 
CTGGAGGAAG ACACTACACC AGAAGAGAAG CGTACCCTGT CAGAGAAGAG GAAGTTGTTG 
ATCAGACAAC ACGAAGACGC CAAAGAGCTC AAGGAGAACC TTGACCGGCG GGAGCGCCTG 
GTTTACAGCA TCATGGCTGC TCACCTCAGC CACGAGAGCC TGGCAGACTA CCAGCACTTT 
GTCAAGATGA AGTCAGCCCT CATCATCGAG CAGCGCAAGC TGGAAGACAA GATAAAACTG 
GGAGAGGAGC AGCTGAAATG TCTGCTGGAT AGTTTATTGT TGGAGCAAAG GCTGCTGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110495881 ( XM_021571386.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021427061.1 Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOf19773
Clone ID Related Accession (Same CDS sequence)	XM_021571386.1
Accession Version	XM_021571386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	protein Shroom2-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTTCC TCCTCTGCTG CATGCCCAGG AAAATGAAAA TGGTGGATAT TGCCTCACAG 
ACCACCATGC CATCAGAATC AGAAACTGAC AAACATGTGG CCAGGAACTT TCTCACCAAG 
ATATTACGAA GTTCTATGAG GAAGAACCGC TTCAAGGGGA GGAATGAACC AGTCTCCCGT 
CCCCATTCAT GGCACTCCTC CAAGTTTACA GAAGACCACC CCGAACCCAC AGAGAGTCAC 
AACGAGCCTC CACCTGCACC CCCACCTGTG TGGCAGGTCA AACATGAAGT CAGTGCATCC 
ACAAAAGACC TTTCCAGCTG CGGGGACCAC GACTCGAATC TACGCCAGTT ATCCAGCCAG 
TTCAGCTCCG TGGGGAATAT GGAGAGAGTA GATCGCCCCT CACACCCCTT CCAACCAGGA 
TGCCTCTCCC CCAGCAGGTA CCACCGCAGC GCTGAGCCTC TCTCTGGGGG TGGAGGGTCT 
GGTGGGAAGA GAGAATCGGC CTTCAGTTGC CTCTCCTCCA CCAGCCCTCC CCCGGAGCCT 
GCTCTGGCCA ACACTGACAC TGCTGCAACT GAGAGCAGCA TGTTCCATAA GGGGGTCCAG 
ACTCAGACCA GTGAGGATGG AAGGCCGGGT GAGCAGCGCC ACAGCCGGTA CCTCCAGCGG 
CCCCAAGGGG ACGGAGGCTC GGAGAGCCCC AGGACAGTCC CAGAGGAGCA GCCTGGCTCC 
CGCTACTCCA GTTCAGGCTC TGGCAGATCG CACATAGGCC CTGTGTGGCA TGTCCCAGAG 
AGGAGGAGGG TAGCAGCGCC CCCCTCTCCT CCCCTGCGCA GTGACAGCTT TGCTGTCACC 
AAGGTGTACC CTGCCTACAC AGAGGGGCCT GGAGCTCCAC CACACGGGCA GATGAAGGCC 
CAGGACAGGT TGGCAGAGGC CCCAGAGAAC AGCAGCTACA GAGGCCGGGG CAACCATATC 
TCTCACGATG AGAACGGTGT AGACCCTAGG TGCAGTTACA ACCCTCCACC TTGCAAGAAA 
GACTTCTTCC ACCCAAACAT AGCAGCAGGA GCACCTGACT ACAACCACAA CCAGCTCAGC 
AACCCAAACA AACTGTCCTC TGTGTCTAGC CAGGACGTGA GACAGAGTCA GTCTCCCTTC 
ACTTGCCTGC CCAACCACCA GCGGCAGTAT AGCGACGAAA GCACTTTCTA CCTGCAGACC 
AGATCCGCCC CACATCCACC GAAGCCGCAG AGCGTCGGTA GCTACTACCA CAGCCTCCAG 
GAGCTCCCTA CCAACTGCAC TAACAGCAGG AACCACGTGA GGTCCTCCAC CACGTCCCTG 
TCCACCTCGA CCATCGACCA GAACTATGAC TGCGGAGGAC ACATCCGGTA CTACTGCATC 
ACAACCAAGC AGCCAGCCCA GCCAGAGACT CATGTTAGAC AGAGCAAATC AGAGGTCTGG 
AGATCTGACA TGGAGCTAGC TAAGGGCTCC AACGACAGGG GCTCTACAAG TTCCTCCCAC 
AAGACCAACA AGGTCAAGTA TCCTCCTAAT CCTCAGCCTG CTTACACCAA CAGCAAGGAG 
CGGAACGGAA ATTTCCAACC GGCCAATGTT CTGCTTTACG GCCACACCAC CTCTAATTCC 
CTATCCTCAT CTGCTAAACC TACCACTGAG GAGAATGAGA GGAAGAGTGA GGGCCAGAGA 
CCTACAGAGG CCCAGCTTAG AAGTTCTTAC TCTCCCAGTC CGCCCAAGGA CCACAAGGTG 
GGACCACTGA GAGAAGACCC TTGGGTCTCT CAGGAGAACC ATAAGATCTC TTCTCAAAAG 
ACACCCATGC TCCACAGTCT GGCTCAGGAG AGTAGAAGCC TAGCCCAGGA GATTAGGAGC 
CCAATGCTTC ATTCTCTAGC CCAGGAGAGT AAGAGCCTGG TGGAGAACAA TCACCCTGCT 
ACGGCACAAC CACCACCATC CAACGGCGGG GGAGAAGCCA ACCACGCCCT ACAGGAGGCT 
TTAACGGACA ACACGGCAAC GGGCAAACTG GCGAGACGCA GCGACCGATA CGCCACCACC 
CTCCGCAACG AGATCCAGCT AAAGAGGGCC CAGCTGCAGA AAAGCCGGAG TGCTGCCACC 
CTGACCTGCC CCAGCGAGAC GGAGGAGCCA GAGGAAGAGG AGACAGACCC GGGGGGGTGG 
AAGTCCACCT CCTCCACCTC CTCTGATGGC TCCTTTTCCA CGTCCTACAA GGACCACCTC 
AAAGAGGCGC AGGCGAGGGT CCTCCAGGCC ACGTCCTTTA GGAGGAGAGA CCTAGAACCT 
CCCGGGTCAG GGTCGGAGGC TCCGTTAACC AAACCCAACT CACACATAGT CTCCCGCATT 
GGCAGCCGCA AGCGTTTCCC TCTGAACAAG AGGGTCCACT CGTTCTCCGA GCCAGACAAG 
ATCAACAAGC TCGGAGTGGA GGGTGAGGGA GAACATCCTG TTGGAACAGC ACGGTCATTT 
ATAGACCGGC GGAAGTTCTT TGAAATGGCT GCTAAACCTT CCTTCTGCAG ACCCATCTCA 
ACCATCCACA AGTCAGGCCA GCAGAGCTCC AGCACCACCT CTGGCACTTT GGAGCGCGGA 
GAGGGCAAGG CCAGAGGGAG AGCCCACTCA GGCGAAACTG AGGAGGGCTG GAGGCCAGGC 
CTTGCCAATG AGACTTCCAG TGACCACCAC CCAGACCCCC TCAGCCCCGC AGGCAGACAG 
GCCCTACTGG AGCAGCAGAG GCTGGGGACC TTCGCTGAGT ACCAGGTCAC CTGGAACATG 
CAGAGGAAGG CTTCAGACAC AAAGCCCCAG GGGAGGTACC ACTCTGCTGA CAACATCCTG 
GACCAGGGTA CAGAGGAGAC GCCTGTCTGT GTCCATGAGA GGTCCAGATC GTCTCCCTCA 
CAAGACTTCC ACACACAGAA GATCCCTATG CCATGGAGAG AGACTGTTGA AAACCCGTAT 
CTGGATCACA GACGGGACCA GCAAGGAGGC AGTTACACCG CCAGAGGGCA GAGCGAGAGC 
AGAGGGCAGC CAGCCCAGCT CCACCACTTC CCACAGCCTC CACAACCGTC TATTCCAAGA 
CTGACCGACA CAGAGCCACA CAGCAGCAGA GACGTGGCCA CCCCCACCCC CCTCCCTCTC 
CCCTCCGAAC ACAGATACAA ACGTGACTCT GCGAACCCCG CCCACAACCT CCCCGCTCTC 
CCCCCGTACC CCTCCAATCA CACCCACAGC TCTGTGTCTG AGCAACCACT ACCACCTCCA 
ACGCCCGCTC CCAAGCCCCA GAATACAGGC CTCATCATCA TGCCACATTG GCCTCTCCTA 
GGCCTGGAAA CCTCAACCAC ATCCTCCTCC TACGGACTGT CTCCCCAGGA ATTCCTGCCT 
GGCCCTTGCA CCCATCAGGC TGAACCATCA TCCAGCAGCA GCTGCTCCTC CCATGGCACA 
GAGCTGGATC CTGCCCCAAA CCAAGCCTGC TCCTTGGGCT CCACCCAGCT CCCCTCTCCC 
TCCCCTCGGA GAACCACTGG ACTGGGCACG GAGGAGGGAG CAGCTGATTC AACACTAGAG 
CCGCCACCCT CCCCCTCTTC CCACTCTCCT TTTTTCTCCT ACCAGCCTGC CAGTCTGACG 
GTTCCCTCCT CAGGTGGGAC TAGTTCTCCC TCTCCTCATT TTGCTCCACA GAGGTTGACG 
GACCAACCCC CTGTCTCTGT GTCTATGCAG GATGAAGCCC AGAGCAGGCC AGAGAACAGG 
ACCAACGCTG TGATGGAGAT GAGCAGTGTA GGGAAGAAGG TCCCTGTTAA AATCGTCCAT 
GCTGAGAGCA CCACAGAGAG GGAGAGCCGC CAGTACCTGC TGCACAGCGA GAAAAATAGG 
GCCCCTGGAG TTTCCGAGGG GCCCGACCTT CCCCCGCCCC TACCGACCAG CCTGCCCTCC 
CCTGAGCCGC AGCCCTACTC CCTGTTCCGT GCCTACACCC CCTACACACG CCATGGGCCC 
CAGAGTCCCC CCAGAGACCC AACCCTCACT GTAGCCCCAG AAGAGGCCCT GTCCCCTGGG 
CAGTCTCAGA CAAACGGCCC CTCCGGTACC TCCACCATGG GTCCCCAGCA GCCTCAGAAG 
AGTAACTCAG ACGAGGATGT GAAGAGAGAG GAGCTGGCCA GAGACATCAT GGACAAGGAT 
AAGTCCCTGG TAGACATCTT GGACCAGAGT AAGATGAAGA CCACCATGGA TCTGATGGAG 
GGGATCTTCC CCCAGGGGGA GCAGATCTTG GATGGGGGGC ACCAGAGGAG GAAAGTCTCC 
CCCAAACAGG GATTGCCGCC CAGGGGAATG GATGACCGGA GAGAGGAGGA GGGCATGTCT 
GCTGCCACAG GGTCCCTGGT GACCAGCTCC TCTTACTACA GCACATCTGC CCCCAAGGCT 
GAGCTGCTCA ACAAGATGAA GGACATGCAG GAGGACGAGC TGGAGGAGCT GGAGCAAGAC 
TCGGAGGACG AACTGGACAT CAACCTGGCC AGCAAGAAGC AAGAGCTGAT TGACAGCCTG 
GGTAAGAAGC TGCAGGTGCT ACGCGAGGCC AGGGAGAGCC TTCAAGAGGA TGTCCATGAC 
AACAACTCTC TGGGGGACGA GGTGGAAGCC GTGGTGCAGA AGGTCTGCAA GCCCAACGAG 
CTGGACAAGT TCAGGATGTT CGTGGGAGAT CTGGACAAGG TGGTCAGTTT GCTGCTGTCC 
CTGTCTGGCC GACTGGCCAG AGTGGAGAAC GCTCTCAACA GTCTGGAGGA AGACACTACA 
CCAGAAGAGA AGCGTACCCT GTCAGAGAAG AGGAAGTTGT TGATCAGACA ACACGAAGAC 
GCCAAAGAGC TCAAGGAGAA CCTTGACCGG CGGGAGCGCC TGGTTTACAG CATCATGGCT 
GCTCACCTCA GCCACGAGAG CCTGGCAGAC TACCAGCACT TTGTCAAGAT GAAGTCAGCC 
CTCATCATCG AGCAGCGCAA GCTGGAAGAC AAGATAAAAC TGGGAGAGGA GCAGCTGAAA 
TGTCTGCTGG ATAGTTTATT GTTGGAGCAA AGGCTGCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021427061.1
CDS	221..5245
Translation	MLFLLCCMPRKMKMVDIASQTTMPSESETDKHVARNFLTKILRSSMRKNRFKGRNEPVSRPHSWHSSKFTEDHPEPTESHNEPPPAPPPVWQVKHEVSASTKDLSSCGDHDSNLRQLSSQFSSVGNMERVDRPSHPFQPGCLSPSRYHRSAEPLSGGGGSGGKRESAFSCLSSTSPPPEPALANTDTAATESSMFHKGVQTQTSEDGRPGEQRHSRYLQRPQGDGGSESPRTVPEEQPGSRYSSSGSGRSHIGPVWHVPERRRVAAPPSPPLRSDSFAVTKVYPAYTEGPGAPPHGQMKAQDRLAEAPENSSYRGRGNHISHDENGVDPRCSYNPPPCKKDFFHPNIAAGAPDYNHNQLSNPNKLSSVSSQDVRQSQSPFTCLPNHQRQYSDESTFYLQTRSAPHPPKPQSVGSYYHSLQELPTNCTNSRNHVRSSTTSLSTSTIDQNYDCGGHIRYYCITTKQPAQPETHVRQSKSEVWRSDMELAKGSNDRGSTSSSHKTNKVKYPPNPQPAYTNSKERNGNFQPANVLLYGHTTSNSLSSSAKPTTEENERKSEGQRPTEAQLRSSYSPSPPKDHKVGPLREDPWVSQENHKISSQKTPMLHSLAQESRSLAQEIRSPMLHSLAQESKSLVENNHPATAQPPPSNGGGEANHALQEALTDNTATGKLARRSDRYATTLRNEIQLKRAQLQKSRSAATLTCPSETEEPEEEETDPGGWKSTSSTSSDGSFSTSYKDHLKEAQARVLQATSFRRRDLEPPGSGSEAPLTKPNSHIVSRIGSRKRFPLNKRVHSFSEPDKINKLGVEGEGEHPVGTARSFIDRRKFFEMAAKPSFCRPISTIHKSGQQSSSTTSGTLERGEGKARGRAHSGETEEGWRPGLANETSSDHHPDPLSPAGRQALLEQQRLGTFAEYQVTWNMQRKASDTKPQGRYHSADNILDQGTEETPVCVHERSRSSPSQDFHTQKIPMPWRETVENPYLDHRRDQQGGSYTARGQSESRGQPAQLHHFPQPPQPSIPRLTDTEPHSSRDVATPTPLPLPSEHRYKRDSANPAHNLPALPPYPSNHTHSSVSEQPLPPPTPAPKPQNTGLIIMPHWPLLGLETSTTSSSYGLSPQEFLPGPCTHQAEPSSSSSCSSHGTELDPAPNQACSLGSTQLPSPSPRRTTGLGTEEGAADSTLEPPPSPSSHSPFFSYQPASLTVPSSGGTSSPSPHFAPQRLTDQPPVSVSMQDEAQSRPENRTNAVMEMSSVGKKVPVKIVHAESTTERESRQYLLHSEKNRAPGVSEGPDLPPPLPTSLPSPEPQPYSLFRAYTPYTRHGPQSPPRDPTLTVAPEEALSPGQSQTNGPSGTSTMGPQQPQKSNSDEDVKREELARDIMDKDKSLVDILDQSKMKTTMDLMEGIFPQGEQILDGGHQRRKVSPKQGLPPRGMDDRREEEGMSAATGSLVTSSSYYSTSAPKAELLNKMKDMQEDELEELEQDSEDELDINLASKKQELIDSLGKKLQVLREARESLQEDVHDNNSLGDEVEAVVQKVCKPNELDKFRMFVGDLDKVVSLLLSLSGRLARVENALNSLEEDTTPEEKRTLSEKRKLLIRQHEDAKELKENLDRRERLVYSIMAAHLSHESLADYQHFVKMKSALIIEQRKLEDKIKLGEEQLKCLLDSLLLEQRLLF

Target ORF information:

RefSeq Version	XM_021571386.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021571386.1

ORF Insert Sequence:

ATGTTGTTCC TCCTCTGCTG CATGCCCAGG AAAATGAAAA TGGTGGATAT TGCCTCACAG 
ACCACCATGC CATCAGAATC AGAAACTGAC AAACATGTGG CCAGGAACTT TCTCACCAAG 
ATATTACGAA GTTCTATGAG GAAGAACCGC TTCAAGGGGA GGAATGAACC AGTCTCCCGT 
CCCCATTCAT GGCACTCCTC CAAGTTTACA GAAGACCACC CCGAACCCAC AGAGAGTCAC 
AACGAGCCTC CACCTGCACC CCCACCTGTG TGGCAGGTCA AACATGAAGT CAGTGCATCC 
ACAAAAGACC TTTCCAGCTG CGGGGACCAC GACTCGAATC TACGCCAGTT ATCCAGCCAG 
TTCAGCTCCG TGGGGAATAT GGAGAGAGTA GATCGCCCCT CACACCCCTT CCAACCAGGA 
TGCCTCTCCC CCAGCAGGTA CCACCGCAGC GCTGAGCCTC TCTCTGGGGG TGGAGGGTCT 
GGTGGGAAGA GAGAATCGGC CTTCAGTTGC CTCTCCTCCA CCAGCCCTCC CCCGGAGCCT 
GCTCTGGCCA ACACTGACAC TGCTGCAACT GAGAGCAGCA TGTTCCATAA GGGGGTCCAG 
ACTCAGACCA GTGAGGATGG AAGGCCGGGT GAGCAGCGCC ACAGCCGGTA CCTCCAGCGG 
CCCCAAGGGG ACGGAGGCTC GGAGAGCCCC AGGACAGTCC CAGAGGAGCA GCCTGGCTCC 
CGCTACTCCA GTTCAGGCTC TGGCAGATCG CACATAGGCC CTGTGTGGCA TGTCCCAGAG 
AGGAGGAGGG TAGCAGCGCC CCCCTCTCCT CCCCTGCGCA GTGACAGCTT TGCTGTCACC 
AAGGTGTACC CTGCCTACAC AGAGGGGCCT GGAGCTCCAC CACACGGGCA GATGAAGGCC 
CAGGACAGGT TGGCAGAGGC CCCAGAGAAC AGCAGCTACA GAGGCCGGGG CAACCATATC 
TCTCACGATG AGAACGGTGT AGACCCTAGG TGCAGTTACA ACCCTCCACC TTGCAAGAAA 
GACTTCTTCC ACCCAAACAT AGCAGCAGGA GCACCTGACT ACAACCACAA CCAGCTCAGC 
AACCCAAACA AACTGTCCTC TGTGTCTAGC CAGGACGTGA GACAGAGTCA GTCTCCCTTC 
ACTTGCCTGC CCAACCACCA GCGGCAGTAT AGCGACGAAA GCACTTTCTA CCTGCAGACC 
AGATCCGCCC CACATCCACC GAAGCCGCAG AGCGTCGGTA GCTACTACCA CAGCCTCCAG 
GAGCTCCCTA CCAACTGCAC TAACAGCAGG AACCACGTGA GGTCCTCCAC CACGTCCCTG 
TCCACCTCGA CCATCGACCA GAACTATGAC TGCGGAGGAC ACATCCGGTA CTACTGCATC 
ACAACCAAGC AGCCAGCCCA GCCAGAGACT CATGTTAGAC AGAGCAAATC AGAGGTCTGG 
AGATCTGACA TGGAGCTAGC TAAGGGCTCC AACGACAGGG GCTCTACAAG TTCCTCCCAC 
AAGACCAACA AGGTCAAGTA TCCTCCTAAT CCTCAGCCTG CTTACACCAA CAGCAAGGAG 
CGGAACGGAA ATTTCCAACC GGCCAATGTT CTGCTTTACG GCCACACCAC CTCTAATTCC 
CTATCCTCAT CTGCTAAACC TACCACTGAG GAGAATGAGA GGAAGAGTGA GGGCCAGAGA 
CCTACAGAGG CCCAGCTTAG AAGTTCTTAC TCTCCCAGTC CGCCCAAGGA CCACAAGGTG 
GGACCACTGA GAGAAGACCC TTGGGTCTCT CAGGAGAACC ATAAGATCTC TTCTCAAAAG 
ACACCCATGC TCCACAGTCT GGCTCAGGAG AGTAGAAGCC TAGCCCAGGA GATTAGGAGC 
CCAATGCTTC ATTCTCTAGC CCAGGAGAGT AAGAGCCTGG TGGAGAACAA TCACCCTGCT 
ACGGCACAAC CACCACCATC CAACGGCGGG GGAGAAGCCA ACCACGCCCT ACAGGAGGCT 
TTAACGGACA ACACGGCAAC GGGCAAACTG GCGAGACGCA GCGACCGATA CGCCACCACC 
CTCCGCAACG AGATCCAGCT AAAGAGGGCC CAGCTGCAGA AAAGCCGGAG TGCTGCCACC 
CTGACCTGCC CCAGCGAGAC GGAGGAGCCA GAGGAAGAGG AGACAGACCC GGGGGGGTGG 
AAGTCCACCT CCTCCACCTC CTCTGATGGC TCCTTTTCCA CGTCCTACAA GGACCACCTC 
AAAGAGGCGC AGGCGAGGGT CCTCCAGGCC ACGTCCTTTA GGAGGAGAGA CCTAGAACCT 
CCCGGGTCAG GGTCGGAGGC TCCGTTAACC AAACCCAACT CACACATAGT CTCCCGCATT 
GGCAGCCGCA AGCGTTTCCC TCTGAACAAG AGGGTCCACT CGTTCTCCGA GCCAGACAAG 
ATCAACAAGC TCGGAGTGGA GGGTGAGGGA GAACATCCTG TTGGAACAGC ACGGTCATTT 
ATAGACCGGC GGAAGTTCTT TGAAATGGCT GCTAAACCTT CCTTCTGCAG ACCCATCTCA 
ACCATCCACA AGTCAGGCCA GCAGAGCTCC AGCACCACCT CTGGCACTTT GGAGCGCGGA 
GAGGGCAAGG CCAGAGGGAG AGCCCACTCA GGCGAAACTG AGGAGGGCTG GAGGCCAGGC 
CTTGCCAATG AGACTTCCAG TGACCACCAC CCAGACCCCC TCAGCCCCGC AGGCAGACAG 
GCCCTACTGG AGCAGCAGAG GCTGGGGACC TTCGCTGAGT ACCAGGTCAC CTGGAACATG 
CAGAGGAAGG CTTCAGACAC AAAGCCCCAG GGGAGGTACC ACTCTGCTGA CAACATCCTG 
GACCAGGGTA CAGAGGAGAC GCCTGTCTGT GTCCATGAGA GGTCCAGATC GTCTCCCTCA 
CAAGACTTCC ACACACAGAA GATCCCTATG CCATGGAGAG AGACTGTTGA AAACCCGTAT 
CTGGATCACA GACGGGACCA GCAAGGAGGC AGTTACACCG CCAGAGGGCA GAGCGAGAGC 
AGAGGGCAGC CAGCCCAGCT CCACCACTTC CCACAGCCTC CACAACCGTC TATTCCAAGA 
CTGACCGACA CAGAGCCACA CAGCAGCAGA GACGTGGCCA CCCCCACCCC CCTCCCTCTC 
CCCTCCGAAC ACAGATACAA ACGTGACTCT GCGAACCCCG CCCACAACCT CCCCGCTCTC 
CCCCCGTACC CCTCCAATCA CACCCACAGC TCTGTGTCTG AGCAACCACT ACCACCTCCA 
ACGCCCGCTC CCAAGCCCCA GAATACAGGC CTCATCATCA TGCCACATTG GCCTCTCCTA 
GGCCTGGAAA CCTCAACCAC ATCCTCCTCC TACGGACTGT CTCCCCAGGA ATTCCTGCCT 
GGCCCTTGCA CCCATCAGGC TGAACCATCA TCCAGCAGCA GCTGCTCCTC CCATGGCACA 
GAGCTGGATC CTGCCCCAAA CCAAGCCTGC TCCTTGGGCT CCACCCAGCT CCCCTCTCCC 
TCCCCTCGGA GAACCACTGG ACTGGGCACG GAGGAGGGAG CAGCTGATTC AACACTAGAG 
CCGCCACCCT CCCCCTCTTC CCACTCTCCT TTTTTCTCCT ACCAGCCTGC CAGTCTGACG 
GTTCCCTCCT CAGGTGGGAC TAGTTCTCCC TCTCCTCATT TTGCTCCACA GAGGTTGACG 
GACCAACCCC CTGTCTCTGT GTCTATGCAG GATGAAGCCC AGAGCAGGCC AGAGAACAGG 
ACCAACGCTG TGATGGAGAT GAGCAGTGTA GGGAAGAAGG TCCCTGTTAA AATCGTCCAT 
GCTGAGAGCA CCACAGAGAG GGAGAGCCGC CAGTACCTGC TGCACAGCGA GAAAAATAGG 
GCCCCTGGAG TTTCCGAGGG GCCCGACCTT CCCCCGCCCC TACCGACCAG CCTGCCCTCC 
CCTGAGCCGC AGCCCTACTC CCTGTTCCGT GCCTACACCC CCTACACACG CCATGGGCCC 
CAGAGTCCCC CCAGAGACCC AACCCTCACT GTAGCCCCAG AAGAGGCCCT GTCCCCTGGG 
CAGTCTCAGA CAAACGGCCC CTCCGGTACC TCCACCATGG GTCCCCAGCA GCCTCAGAAG 
AGTAACTCAG ACGAGGATGT GAAGAGAGAG GAGCTGGCCA GAGACATCAT GGACAAGGAT 
AAGTCCCTGG TAGACATCTT GGACCAGAGT AAGATGAAGA CCACCATGGA TCTGATGGAG 
GGGATCTTCC CCCAGGGGGA GCAGATCTTG GATGGGGGGC ACCAGAGGAG GAAAGTCTCC 
CCCAAACAGG GATTGCCGCC CAGGGGAATG GATGACCGGA GAGAGGAGGA GGGCATGTCT 
GCTGCCACAG GGTCCCTGGT GACCAGCTCC TCTTACTACA GCACATCTGC CCCCAAGGCT 
GAGCTGCTCA ACAAGATGAA GGACATGCAG GAGGACGAGC TGGAGGAGCT GGAGCAAGAC 
TCGGAGGACG AACTGGACAT CAACCTGGCC AGCAAGAAGC AAGAGCTGAT TGACAGCCTG 
GGTAAGAAGC TGCAGGTGCT ACGCGAGGCC AGGGAGAGCC TTCAAGAGGA TGTCCATGAC 
AACAACTCTC TGGGGGACGA GGTGGAAGCC GTGGTGCAGA AGGTCTGCAA GCCCAACGAG 
CTGGACAAGT TCAGGATGTT CGTGGGAGAT CTGGACAAGG TGGTCAGTTT GCTGCTGTCC 
CTGTCTGGCC GACTGGCCAG AGTGGAGAAC GCTCTCAACA GTCTGGAGGA AGACACTACA 
CCAGAAGAGA AGCGTACCCT GTCAGAGAAG AGGAAGTTGT TGATCAGACA ACACGAAGAC 
GCCAAAGAGC TCAAGGAGAA CCTTGACCGG CGGGAGCGCC TGGTTTACAG CATCATGGCT 
GCTCACCTCA GCCACGAGAG CCTGGCAGAC TACCAGCACT TTGTCAAGAT GAAGTCAGCC 
CTCATCATCG AGCAGCGCAA GCTGGAAGAC AAGATAAAAC TGGGAGAGGA GCAGCTGAAA 
TGTCTGCTGG ATAGTTTATT GTTGGAGCAA AGGCTGCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110495881 ( XM_021571387.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021427062.1 Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X4, mRNA.

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CloneID	OOf19774
Clone ID Related Accession (Same CDS sequence)	XM_021571387.1
Accession Version	XM_021571387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	protein Shroom2-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTTCC TCCTCTGCTG CATGCCCAGG AAAATGAAAA TGGTGGATAT TGCCTCACAG 
ACCACCATGC CATCAGAATC AGAAACTGAC AAACATGTGG CCAGGAACTT TCTCACCAAG 
ATATTACGAA GTTCTATGAG GAGGAATGAA CCAGTCTCCC GTCCCCATTC ATGGCACTCC 
TCCAAGTTTA CAGAAGACCA CCCCGAACCC ACAGAGAGTC ACAACGAGCC TCCACCTGCA 
CCCCCACCTG TGTGGCAGGT CAAACATGAA GTCAGTGCAT CCACAAAAGA CCTTTCCAGC 
TGCGGGGACC ACGACTCGAA TCTACGCCAG TTATCCAGCC AGTTCAGCTC CGTGGGGAAT 
ATGGAGAGAG TAGATCGCCC CTCACACCCC TTCCAACCAG GATGCCTCTC CCCCAGCAGG 
TACCACCGCA GCGCTGAGCC TCTCTCTGGG GGTGGAGGGT CTGGTGGGAA GAGAGAATCG 
GCCTTCAGTT GCCTCTCCTC CACCAGCCCT CCCCCGGAGC CTGCTCTGGC CAACACTGAC 
ACTGCTGCAA CTGAGAGCAG CATGTTCCAT AAGGGGGTCC AGACTCAGAC CAGTGAGGAT 
GGAAGGCCGG GTGAGCAGCG CCACAGCCGG TACCTCCAGC GGCCCCAAGG GGACGGAGGC 
TCGGAGAGCC CCAGGACAGT CCCAGAGGAG CAGCCTGGCT CCCGCTACTC CAGTTCAGGC 
TCTGGCAGAT CGCACATAGG CCCTGTGTGG CATGTCCCAG AGAGGAGGAG GGTAGCAGCG 
CCCCCCTCTC CTCCCCTGCG CAGTGACAGC TTTGCTGTCA CCAAGGTGTA CCCTGCCTAC 
ACAGAGGGGC CTGGAGCTCC ACCACACGGG CAGATGAAGG CCCAGGACAG GTTGGCAGAG 
GCCCCAGAGA ACAGCAGCTA CAGAGGCCGG GGCAACCATA TCTCTCACGA TGAGAACGGT 
GTAGACCCTA GGTGCAGTTA CAACCCTCCA CCTTGCAAGA AAGACTTCTT CCACCCAAAC 
ATAGCAGCAG GAGCACCTGA CTACAACCAC AACCAGCTCA GCAACCCAAA CAAACTGTCC 
TCTGTGTCTA GCCAGGACGT GAGACAGAGT CAGTCTCCCT TCACTTGCCT GCCCAACCAC 
CAGCGGCAGT ATAGCGACGA AAGCACTTTC TACCTGCAGA CCAGATCCGC CCCACATCCA 
CCGAAGCCGC AGAGCGTCGG TAGCTACTAC CACAGCCTCC AGGAGCTCCC TACCAACTGC 
ACTAACAGCA GGAACCACGT GAGGTCCTCC ACCACGTCCC TGTCCACCTC GACCATCGAC 
CAGAACTATG ACTGCGGAGG ACACATCCGG TACTACTGCA TCACAACCAA GCAGCCAGCC 
CAGCCAGAGA CTCATGTTAG ACAGAGCAAA TCAGAGGTCT GGAGATCTGA CATGGAGCTA 
GCTAAGGGCT CCAACGACAG GGGCTCTACA AGTTCCTCCC ACAAGACCAA CAAGGTCAAG 
TATCCTCCTA ATCCTCAGCC TGCTTACACC AACAGCAAGG AGCGGAACGG AAATTTCCAA 
CCGGCCAATG TTCTGCTTTA CGGCCACACC ACCTCTAATT CCCTATCCTC ATCTGCTAAA 
CCTACCACTG AGGAGAATGA GAGGAAGAGT GAGGGCCAGA GACCTACAGA GGCCCAGCTT 
AGAAGTTCTT ACTCTCCCAG TCCGCCCAAG GACCACAAGG TGGGACCACT GAGAGAAGAC 
CCTTGGGTCT CTCAGGAGAA CCATAAGATC TCTTCTCAAA AGACACCCAT GCTCCACAGT 
CTGGCTCAGG AGAGTAGAAG CCTAGCCCAG GAGATTAGGA GCCCAATGCT TCATTCTCTA 
GCCCAGGAGA GTAAGAGCCT GGTGGAGAAC AATCACCCTG CTACGGCACA ACCACCACCA 
TCCAACGGCG GGGGAGAAGC CAACCACGCC CTACAGGAGG CTTTAACGGA CAACACGGCA 
ACGGGCAAAC TGGCGAGACG CAGCGACCGA TACGCCACCA CCCTCCGCAA CGAGATCCAG 
CTAAAGAGGG CCCAGCTGCA GAAAAGCCGG AGTGCTGCCA CCCTGACCTG CCCCAGCGAG 
ACGGAGGAGC CAGAGGAAGA GGAGACAGAC CCGGGGGGGT GGAAGTCCAC CTCCTCCACC 
TCCTCTGATG GCTCCTTTTC CACGTCCTAC AAGGACCACC TCAAAGAGGC GCAGGCGAGG 
GTCCTCCAGG CCACGTCCTT TAGGAGGAGA GACCTAGAAC CTCCCGGGTC AGGGTCGGAG 
GCTCCGTTAA CCAAACCCAA CTCACACATA GTCTCCCGCA TTGGCAGCCG CAAGCGTTTC 
CCTCTGAACA AGAGGGTCCA CTCGTTCTCC GAGCCAGACA AGATCAACAA GCTCGGAGTG 
GAGGGTGAGG GAGAACATCC TGTTGGAACA GCACGGTCAT TTATAGACCG GCGGAAGTTC 
TTTGAAATGG CTGCTAAACC TTCCTTCTGC AGACCCATCT CAACCATCCA CAAGTCAGGC 
CAGCAGAGCT CCAGCACCAC CTCTGGCACT TTGGAGCGCG GAGAGGGCAA GGCCAGAGGG 
AGAGCCCACT CAGGCGAAAC TGAGGAGGGC TGGAGGCCAG GCCTTGCCAA TGAGACTTCC 
AGTGACCACC ACCCAGACCC CCTCAGCCCC GCAGGCAGAC AGGCCCTACT GGAGCAGCAG 
AGGCTGGGGA CCTTCGCTGA GTACCAGGTC ACCTGGAACA TGCAGAGGAA GGCTTCAGAC 
ACAAAGCCCC AGGGGAGGTA CCACTCTGCT GACAACATCC TGGACCAGGG TACAGAGGAG 
ACGCCTGTCT GTGTCCATGA GAGGTCCAGA TCGTCTCCCT CACAAGACTT CCACACACAG 
AAGATCCCTA TGCCATGGAG AGAGACTGTT GAAAACCCGT ATCTGGATCA CAGACGGGAC 
CAGCAAGGAG GCAGTTACAC CGCCAGAGGG CAGAGCGAGA GCAGAGGGCA GCCAGCCCAG 
CTCCACCACT TCCCACAGCC TCCACAACCG TCTATTCCAA GACTGACCGA CACAGAGCCA 
CACAGCAGCA GAGACGTGGC CACCCCCACC CCCCTCCCTC TCCCCTCCGA ACACAGATAC 
AAACGTGACT CTGCGAACCC CGCCCACAAC CTCCCCGCTC TCCCCCCGTA CCCCTCCAAT 
CACACCCACA GCTCTGTGTC TGAGCAACCA CTACCACCTC CAACGCCCGC TCCCAAGCCC 
CAGAATACAG GCCTCATCAT CATGCCACAT TGGCCTCTCC TAGGCCTGGA AACCTCAACC 
ACATCCTCCT CCTACGGACT GTCTCCCCAG GAATTCCTGC CTGGCCCTTG CACCCATCAG 
GCTGAACCAT CATCCAGCAG CAGCTGCTCC TCCCATGGCA CAGAGCTGGA TCCTGCCCCA 
AACCAAGCCT GCTCCTTGGG CTCCACCCAG CTCCCCTCTC CCTCCCCTCG GAGAACCACT 
GGACTGGGCA CGGAGGAGGG AGCAGCTGAT TCAACACTAG AGCCGCCACC CTCCCCCTCT 
TCCCACTCTC CTTTTTTCTC CTACCAGCCT GCCAGTCTGA CGGTTCCCTC CTCAGGTGGG 
ACTAGTTCTC CCTCTCCTCA TTTTGCTCCA CAGAGGTTGA CGGACCAACC CCCTGTCTCT 
GTGTCTATGC AGGATGAAGC CCAGAGCAGG CCAGAGAACA GGACCAACGC TGTGATGGAG 
ATGAGCAGTG TAGGGAAGAA GGTCCCTGTT AAAATCGTCC ATGCTGAGAG CACCACAGAG 
AGGGAGAGCC GCCAGTACCT GCTGCACAGC GAGAAAAATA GGGCCCCTGG AGTTTCCGAG 
GGGCCCGACC TTCCCCCGCC CCTACCGACC AGCCTGCCCT CCCCTGAGCC GCAGCCCTAC 
TCCCTGTTCC GTGCCTACAC CCCCTACACA CGCCATGGGC CCCAGAGTCC CCCCAGAGAC 
CCAACCCTCA CTGTAGCCCC AGAAGAGGCC CTGTCCCCTG GGCAGTCTCA GACAAACGGC 
CCCTCCGGTA CCTCCACCAT GGGTCCCCAG CAGCCTCAGA AGAGTAACTC AGACGAGGAT 
GTGAAGAGAG AGGAGCTGGC CAGAGACATC ATGGACAAGG ATAAGTCCCT GGTAGACATC 
TTGGACCAGA GTAAGATGAA GACCACCATG GATCTGATGG AGGGGATCTT CCCCCAGGGG 
GAGCAGATCT TGGATGGGGG GCACCAGAGG AGGAAAGTCT CCCCCAAACA GGGATTGCCG 
CCCAGGGGAA TGGATGACCG GAGAGAGGAG GAGGGCATGT CTGCTGCCAC AGGGTCCCTG 
GTGACCAGCT CCTCTTACTA CAGCACATCT GCCCCCAAGG CTGAGCTGCT CAACAAGATG 
AAGGACATGC AGGAGGACGA GCTGGAGGAG CTGGAGCAAG ACTCGGAGGA CGAACTGGAC 
ATCAACCTGG CCAGCAAGAA GCAAGAGCTG ATTGACAGCC TGGGTAAGAA GCTGCAGGTG 
CTACGCGAGG CCAGGGAGAG CCTTCAAGAG GATGTCCATG ACAACAACTC TCTGGGGGAC 
GAGGTGGAAG CCGTGGTGCA GAAGGTCTGC AAGCCCAACG AGCTGGACAA GTTCAGGATG 
TTCGTGGGAG ATCTGGACAA GGTGGTCAGT TTGCTGCTGT CCCTGTCTGG CCGACTGGCC 
AGAGTGGAGA ACGCTCTCAA CAGTCTGGAG GAAGACACTA CACCAGAAGA GAAGCGTACC 
CTGTCAGAGA AGAGGAAGTT GTTGATCAGA CAACACGAAG ACGCCAAAGA GCTCAAGGAG 
AACCTTGACC GGCGGGAGCG CCTGGTTTAC AGCATCATGG CTGCTCACCT CAGCCACGAG 
AGCCTGGCAG ACTACCAGCA CTTTGTCAAG ATGAAGTCAG CCCTCATCAT CGAGCAGCGC 
AAGCTGGAAG ACAAGATAAA ACTGGGAGAG GAGCAGCTGA AATGTCTGCT GGATAGTTTA 
TTGTTGGAGC AAAGGCTGCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021427062.1
CDS	221..5227
Translation	MLFLLCCMPRKMKMVDIASQTTMPSESETDKHVARNFLTKILRSSMRRNEPVSRPHSWHSSKFTEDHPEPTESHNEPPPAPPPVWQVKHEVSASTKDLSSCGDHDSNLRQLSSQFSSVGNMERVDRPSHPFQPGCLSPSRYHRSAEPLSGGGGSGGKRESAFSCLSSTSPPPEPALANTDTAATESSMFHKGVQTQTSEDGRPGEQRHSRYLQRPQGDGGSESPRTVPEEQPGSRYSSSGSGRSHIGPVWHVPERRRVAAPPSPPLRSDSFAVTKVYPAYTEGPGAPPHGQMKAQDRLAEAPENSSYRGRGNHISHDENGVDPRCSYNPPPCKKDFFHPNIAAGAPDYNHNQLSNPNKLSSVSSQDVRQSQSPFTCLPNHQRQYSDESTFYLQTRSAPHPPKPQSVGSYYHSLQELPTNCTNSRNHVRSSTTSLSTSTIDQNYDCGGHIRYYCITTKQPAQPETHVRQSKSEVWRSDMELAKGSNDRGSTSSSHKTNKVKYPPNPQPAYTNSKERNGNFQPANVLLYGHTTSNSLSSSAKPTTEENERKSEGQRPTEAQLRSSYSPSPPKDHKVGPLREDPWVSQENHKISSQKTPMLHSLAQESRSLAQEIRSPMLHSLAQESKSLVENNHPATAQPPPSNGGGEANHALQEALTDNTATGKLARRSDRYATTLRNEIQLKRAQLQKSRSAATLTCPSETEEPEEEETDPGGWKSTSSTSSDGSFSTSYKDHLKEAQARVLQATSFRRRDLEPPGSGSEAPLTKPNSHIVSRIGSRKRFPLNKRVHSFSEPDKINKLGVEGEGEHPVGTARSFIDRRKFFEMAAKPSFCRPISTIHKSGQQSSSTTSGTLERGEGKARGRAHSGETEEGWRPGLANETSSDHHPDPLSPAGRQALLEQQRLGTFAEYQVTWNMQRKASDTKPQGRYHSADNILDQGTEETPVCVHERSRSSPSQDFHTQKIPMPWRETVENPYLDHRRDQQGGSYTARGQSESRGQPAQLHHFPQPPQPSIPRLTDTEPHSSRDVATPTPLPLPSEHRYKRDSANPAHNLPALPPYPSNHTHSSVSEQPLPPPTPAPKPQNTGLIIMPHWPLLGLETSTTSSSYGLSPQEFLPGPCTHQAEPSSSSSCSSHGTELDPAPNQACSLGSTQLPSPSPRRTTGLGTEEGAADSTLEPPPSPSSHSPFFSYQPASLTVPSSGGTSSPSPHFAPQRLTDQPPVSVSMQDEAQSRPENRTNAVMEMSSVGKKVPVKIVHAESTTERESRQYLLHSEKNRAPGVSEGPDLPPPLPTSLPSPEPQPYSLFRAYTPYTRHGPQSPPRDPTLTVAPEEALSPGQSQTNGPSGTSTMGPQQPQKSNSDEDVKREELARDIMDKDKSLVDILDQSKMKTTMDLMEGIFPQGEQILDGGHQRRKVSPKQGLPPRGMDDRREEEGMSAATGSLVTSSSYYSTSAPKAELLNKMKDMQEDELEELEQDSEDELDINLASKKQELIDSLGKKLQVLREARESLQEDVHDNNSLGDEVEAVVQKVCKPNELDKFRMFVGDLDKVVSLLLSLSGRLARVENALNSLEEDTTPEEKRTLSEKRKLLIRQHEDAKELKENLDRRERLVYSIMAAHLSHESLADYQHFVKMKSALIIEQRKLEDKIKLGEEQLKCLLDSLLLEQRLLF

Target ORF information:

RefSeq Version	XM_021571387.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021571387.1

ORF Insert Sequence:

ATGTTGTTCC TCCTCTGCTG CATGCCCAGG AAAATGAAAA TGGTGGATAT TGCCTCACAG 
ACCACCATGC CATCAGAATC AGAAACTGAC AAACATGTGG CCAGGAACTT TCTCACCAAG 
ATATTACGAA GTTCTATGAG GAGGAATGAA CCAGTCTCCC GTCCCCATTC ATGGCACTCC 
TCCAAGTTTA CAGAAGACCA CCCCGAACCC ACAGAGAGTC ACAACGAGCC TCCACCTGCA 
CCCCCACCTG TGTGGCAGGT CAAACATGAA GTCAGTGCAT CCACAAAAGA CCTTTCCAGC 
TGCGGGGACC ACGACTCGAA TCTACGCCAG TTATCCAGCC AGTTCAGCTC CGTGGGGAAT 
ATGGAGAGAG TAGATCGCCC CTCACACCCC TTCCAACCAG GATGCCTCTC CCCCAGCAGG 
TACCACCGCA GCGCTGAGCC TCTCTCTGGG GGTGGAGGGT CTGGTGGGAA GAGAGAATCG 
GCCTTCAGTT GCCTCTCCTC CACCAGCCCT CCCCCGGAGC CTGCTCTGGC CAACACTGAC 
ACTGCTGCAA CTGAGAGCAG CATGTTCCAT AAGGGGGTCC AGACTCAGAC CAGTGAGGAT 
GGAAGGCCGG GTGAGCAGCG CCACAGCCGG TACCTCCAGC GGCCCCAAGG GGACGGAGGC 
TCGGAGAGCC CCAGGACAGT CCCAGAGGAG CAGCCTGGCT CCCGCTACTC CAGTTCAGGC 
TCTGGCAGAT CGCACATAGG CCCTGTGTGG CATGTCCCAG AGAGGAGGAG GGTAGCAGCG 
CCCCCCTCTC CTCCCCTGCG CAGTGACAGC TTTGCTGTCA CCAAGGTGTA CCCTGCCTAC 
ACAGAGGGGC CTGGAGCTCC ACCACACGGG CAGATGAAGG CCCAGGACAG GTTGGCAGAG 
GCCCCAGAGA ACAGCAGCTA CAGAGGCCGG GGCAACCATA TCTCTCACGA TGAGAACGGT 
GTAGACCCTA GGTGCAGTTA CAACCCTCCA CCTTGCAAGA AAGACTTCTT CCACCCAAAC 
ATAGCAGCAG GAGCACCTGA CTACAACCAC AACCAGCTCA GCAACCCAAA CAAACTGTCC 
TCTGTGTCTA GCCAGGACGT GAGACAGAGT CAGTCTCCCT TCACTTGCCT GCCCAACCAC 
CAGCGGCAGT ATAGCGACGA AAGCACTTTC TACCTGCAGA CCAGATCCGC CCCACATCCA 
CCGAAGCCGC AGAGCGTCGG TAGCTACTAC CACAGCCTCC AGGAGCTCCC TACCAACTGC 
ACTAACAGCA GGAACCACGT GAGGTCCTCC ACCACGTCCC TGTCCACCTC GACCATCGAC 
CAGAACTATG ACTGCGGAGG ACACATCCGG TACTACTGCA TCACAACCAA GCAGCCAGCC 
CAGCCAGAGA CTCATGTTAG ACAGAGCAAA TCAGAGGTCT GGAGATCTGA CATGGAGCTA 
GCTAAGGGCT CCAACGACAG GGGCTCTACA AGTTCCTCCC ACAAGACCAA CAAGGTCAAG 
TATCCTCCTA ATCCTCAGCC TGCTTACACC AACAGCAAGG AGCGGAACGG AAATTTCCAA 
CCGGCCAATG TTCTGCTTTA CGGCCACACC ACCTCTAATT CCCTATCCTC ATCTGCTAAA 
CCTACCACTG AGGAGAATGA GAGGAAGAGT GAGGGCCAGA GACCTACAGA GGCCCAGCTT 
AGAAGTTCTT ACTCTCCCAG TCCGCCCAAG GACCACAAGG TGGGACCACT GAGAGAAGAC 
CCTTGGGTCT CTCAGGAGAA CCATAAGATC TCTTCTCAAA AGACACCCAT GCTCCACAGT 
CTGGCTCAGG AGAGTAGAAG CCTAGCCCAG GAGATTAGGA GCCCAATGCT TCATTCTCTA 
GCCCAGGAGA GTAAGAGCCT GGTGGAGAAC AATCACCCTG CTACGGCACA ACCACCACCA 
TCCAACGGCG GGGGAGAAGC CAACCACGCC CTACAGGAGG CTTTAACGGA CAACACGGCA 
ACGGGCAAAC TGGCGAGACG CAGCGACCGA TACGCCACCA CCCTCCGCAA CGAGATCCAG 
CTAAAGAGGG CCCAGCTGCA GAAAAGCCGG AGTGCTGCCA CCCTGACCTG CCCCAGCGAG 
ACGGAGGAGC CAGAGGAAGA GGAGACAGAC CCGGGGGGGT GGAAGTCCAC CTCCTCCACC 
TCCTCTGATG GCTCCTTTTC CACGTCCTAC AAGGACCACC TCAAAGAGGC GCAGGCGAGG 
GTCCTCCAGG CCACGTCCTT TAGGAGGAGA GACCTAGAAC CTCCCGGGTC AGGGTCGGAG 
GCTCCGTTAA CCAAACCCAA CTCACACATA GTCTCCCGCA TTGGCAGCCG CAAGCGTTTC 
CCTCTGAACA AGAGGGTCCA CTCGTTCTCC GAGCCAGACA AGATCAACAA GCTCGGAGTG 
GAGGGTGAGG GAGAACATCC TGTTGGAACA GCACGGTCAT TTATAGACCG GCGGAAGTTC 
TTTGAAATGG CTGCTAAACC TTCCTTCTGC AGACCCATCT CAACCATCCA CAAGTCAGGC 
CAGCAGAGCT CCAGCACCAC CTCTGGCACT TTGGAGCGCG GAGAGGGCAA GGCCAGAGGG 
AGAGCCCACT CAGGCGAAAC TGAGGAGGGC TGGAGGCCAG GCCTTGCCAA TGAGACTTCC 
AGTGACCACC ACCCAGACCC CCTCAGCCCC GCAGGCAGAC AGGCCCTACT GGAGCAGCAG 
AGGCTGGGGA CCTTCGCTGA GTACCAGGTC ACCTGGAACA TGCAGAGGAA GGCTTCAGAC 
ACAAAGCCCC AGGGGAGGTA CCACTCTGCT GACAACATCC TGGACCAGGG TACAGAGGAG 
ACGCCTGTCT GTGTCCATGA GAGGTCCAGA TCGTCTCCCT CACAAGACTT CCACACACAG 
AAGATCCCTA TGCCATGGAG AGAGACTGTT GAAAACCCGT ATCTGGATCA CAGACGGGAC 
CAGCAAGGAG GCAGTTACAC CGCCAGAGGG CAGAGCGAGA GCAGAGGGCA GCCAGCCCAG 
CTCCACCACT TCCCACAGCC TCCACAACCG TCTATTCCAA GACTGACCGA CACAGAGCCA 
CACAGCAGCA GAGACGTGGC CACCCCCACC CCCCTCCCTC TCCCCTCCGA ACACAGATAC 
AAACGTGACT CTGCGAACCC CGCCCACAAC CTCCCCGCTC TCCCCCCGTA CCCCTCCAAT 
CACACCCACA GCTCTGTGTC TGAGCAACCA CTACCACCTC CAACGCCCGC TCCCAAGCCC 
CAGAATACAG GCCTCATCAT CATGCCACAT TGGCCTCTCC TAGGCCTGGA AACCTCAACC 
ACATCCTCCT CCTACGGACT GTCTCCCCAG GAATTCCTGC CTGGCCCTTG CACCCATCAG 
GCTGAACCAT CATCCAGCAG CAGCTGCTCC TCCCATGGCA CAGAGCTGGA TCCTGCCCCA 
AACCAAGCCT GCTCCTTGGG CTCCACCCAG CTCCCCTCTC CCTCCCCTCG GAGAACCACT 
GGACTGGGCA CGGAGGAGGG AGCAGCTGAT TCAACACTAG AGCCGCCACC CTCCCCCTCT 
TCCCACTCTC CTTTTTTCTC CTACCAGCCT GCCAGTCTGA CGGTTCCCTC CTCAGGTGGG 
ACTAGTTCTC CCTCTCCTCA TTTTGCTCCA CAGAGGTTGA CGGACCAACC CCCTGTCTCT 
GTGTCTATGC AGGATGAAGC CCAGAGCAGG CCAGAGAACA GGACCAACGC TGTGATGGAG 
ATGAGCAGTG TAGGGAAGAA GGTCCCTGTT AAAATCGTCC ATGCTGAGAG CACCACAGAG 
AGGGAGAGCC GCCAGTACCT GCTGCACAGC GAGAAAAATA GGGCCCCTGG AGTTTCCGAG 
GGGCCCGACC TTCCCCCGCC CCTACCGACC AGCCTGCCCT CCCCTGAGCC GCAGCCCTAC 
TCCCTGTTCC GTGCCTACAC CCCCTACACA CGCCATGGGC CCCAGAGTCC CCCCAGAGAC 
CCAACCCTCA CTGTAGCCCC AGAAGAGGCC CTGTCCCCTG GGCAGTCTCA GACAAACGGC 
CCCTCCGGTA CCTCCACCAT GGGTCCCCAG CAGCCTCAGA AGAGTAACTC AGACGAGGAT 
GTGAAGAGAG AGGAGCTGGC CAGAGACATC ATGGACAAGG ATAAGTCCCT GGTAGACATC 
TTGGACCAGA GTAAGATGAA GACCACCATG GATCTGATGG AGGGGATCTT CCCCCAGGGG 
GAGCAGATCT TGGATGGGGG GCACCAGAGG AGGAAAGTCT CCCCCAAACA GGGATTGCCG 
CCCAGGGGAA TGGATGACCG GAGAGAGGAG GAGGGCATGT CTGCTGCCAC AGGGTCCCTG 
GTGACCAGCT CCTCTTACTA CAGCACATCT GCCCCCAAGG CTGAGCTGCT CAACAAGATG 
AAGGACATGC AGGAGGACGA GCTGGAGGAG CTGGAGCAAG ACTCGGAGGA CGAACTGGAC 
ATCAACCTGG CCAGCAAGAA GCAAGAGCTG ATTGACAGCC TGGGTAAGAA GCTGCAGGTG 
CTACGCGAGG CCAGGGAGAG CCTTCAAGAG GATGTCCATG ACAACAACTC TCTGGGGGAC 
GAGGTGGAAG CCGTGGTGCA GAAGGTCTGC AAGCCCAACG AGCTGGACAA GTTCAGGATG 
TTCGTGGGAG ATCTGGACAA GGTGGTCAGT TTGCTGCTGT CCCTGTCTGG CCGACTGGCC 
AGAGTGGAGA ACGCTCTCAA CAGTCTGGAG GAAGACACTA CACCAGAAGA GAAGCGTACC 
CTGTCAGAGA AGAGGAAGTT GTTGATCAGA CAACACGAAG ACGCCAAAGA GCTCAAGGAG 
AACCTTGACC GGCGGGAGCG CCTGGTTTAC AGCATCATGG CTGCTCACCT CAGCCACGAG 
AGCCTGGCAG ACTACCAGCA CTTTGTCAAG ATGAAGTCAG CCCTCATCAT CGAGCAGCGC 
AAGCTGGAAG ACAAGATAAA ACTGGGAGAG GAGCAGCTGA AATGTCTGCT GGATAGTTTA 
TTGTTGGAGC AAAGGCTGCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110495881 ( XM_021571389.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021427064.1 Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X5, mRNA.

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CloneID	OOf19775
Clone ID Related Accession (Same CDS sequence)	XM_021571389.1 , XM_021571390.1
Accession Version	XM_021571389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	protein Shroom2-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGG TGGATATTGC CTCACAGACC ACCATGCCAT CAGAATCAGA AACTGACAAA 
CATGTGGCCA GGAACTTTCT CACCAAGATA TTACGAAGTT CTATGAGGAA GAACCGCTTC 
AAGGGGAGGA ATGAACCAGT CTCCCGTCCC CATTCATGGC ACTCCTCCAA GTTTACAGAA 
GACCACCCCG AACCCACAGA GAGTCACAAC GAGCCTCCAC CTGCACCCCC ACCTGTGTGG 
CAGGTCAAAC ATGAAGTCAG TGCATCCACA AAAGACCTTT CCAGCTGCGG GGACCACGAC 
TCGAATCTAC GCCAGTTATC CAGCCAGTTC AGCTCCGTGG GGAATATGGA GAGAGTAGAT 
CGCCCCTCAC ACCCCTTCCA ACCAGGATGC CTCTCCCCCA GCAGGTACCA CCGCAGCGCT 
GAGCCTCTCT CTGGGGGTGG AGGGTCTGGT GGGAAGAGAG AATCGGCCTT CAGTTGCCTC 
TCCTCCACCA GCCCTCCCCC GGAGCCTGCT CTGGCCAACA CTGACACTGC TGCAACTGAG 
AGCAGCATGT TCCATAAGGG GGTCCAGACT CAGACCAGTG AGGATGGAAG GCCGGGTGAG 
CAGCGCCACA GCCGGTACCT CCAGCGGCCC CAAGGGGACG GAGGCTCGGA GAGCCCCAGG 
ACAGTCCCAG AGGAGCAGCC TGGCTCCCGC TACTCCAGTT CAGGCTCTGG CAGATCGCAC 
ATAGGCCCTG TGTGGCATGT CCCAGAGAGG AGGAGGGTAG CAGCGCCCCC CTCTCCTCCC 
CTGCGCAGTG ACAGCTTTGC TGTCACCAAG GTGTACCCTG CCTACACAGA GGGGCCTGGA 
GCTCCACCAC ACGGGCAGAT GAAGGCCCAG GACAGGTTGG CAGAGGCCCC AGAGAACAGC 
AGCTACAGAG GCCGGGGCAA CCATATCTCT CACGATGAGA ACGGTGTAGA CCCTAGGTGC 
AGTTACAACC CTCCACCTTG CAAGAAAGAC TTCTTCCACC CAAACATAGC AGCAGGAGCA 
CCTGACTACA ACCACAACCA GCTCAGCAAC CCAAACAAAC TGTCCTCTGT GTCTAGCCAG 
GACGTGAGAC AGAGTCAGTC TCCCTTCACT TGCCTGCCCA ACCACCAGCG GCAGTATAGC 
GACGAAAGCA CTTTCTACCT GCAGACCAGA TCCGCCCCAC ATCCACCGAA GCCGCAGAGC 
GTCGGTAGCT ACTACCACAG CCTCCAGGAG CTCCCTACCA ACTGCACTAA CAGCAGGAAC 
CACGTGAGGT CCTCCACCAC GTCCCTGTCC ACCTCGACCA TCGACCAGAA CTATGACTGC 
GGAGGACACA TCCGGTACTA CTGCATCACA ACCAAGCAGC CAGCCCAGCC AGAGACTCAT 
GTTAGACAGA GCAAATCAGA GGTCTGGAGA TCTGACATGG AGCTAGCTAA GGGCTCCAAC 
GACAGGGGCT CTACAAGTTC CTCCCACAAG ACCAACAAGG TCAAGTATCC TCCTAATCCT 
CAGCCTGCTT ACACCAACAG CAAGGAGCGG AACGGAAATT TCCAACCGGC CAATGTTCTG 
CTTTACGGCC ACACCACCTC TAATTCCCTA TCCTCATCTG CTAAACCTAC CACTGAGGAG 
AATGAGAGGA AGAGTGAGGG CCAGAGACCT ACAGAGGCCC AGCTTAGAAG TTCTTACTCT 
CCCAGTCCGC CCAAGGACCA CAAGGTGGGA CCACTGAGAG AAGACCCTTG GGTCTCTCAG 
GAGAACCATA AGATCTCTTC TCAAAAGACA CCCATGCTCC ACAGTCTGGC TCAGGAGAGT 
AGAAGCCTAG CCCAGGAGAT TAGGAGCCCA ATGCTTCATT CTCTAGCCCA GGAGAGTAAG 
AGCCTGGTGG AGAACAATCA CCCTGCTACG GCACAACCAC CACCATCCAA CGGCGGGGGA 
GAAGCCAACC ACGCCCTACA GGAGGCTTTA ACGGACAACA CGGCAACGGG CAAACTGGCG 
AGACGCAGCG ACCGATACGC CACCACCCTC CGCAACGAGA TCCAGCTAAA GAGGGCCCAG 
CTGCAGAAAA GCCGGAGTGC TGCCACCCTG ACCTGCCCCA GCGAGACGGA GGAGCCAGAG 
GAAGAGGAGA CAGACCCGGG GGGGTGGAAG TCCACCTCCT CCACCTCCTC TGATGGCTCC 
TTTTCCACGT CCTACAAGGA CCACCTCAAA GAGGCGCAGG CGAGGGTCCT CCAGGCCACG 
TCCTTTAGGA GGAGAGACCT AGAACCTCCC GGGTCAGGGT CGGAGGCTCC GTTAACCAAA 
CCCAACTCAC ACATAGTCTC CCGCATTGGC AGCCGCAAGC GTTTCCCTCT GAACAAGAGG 
GTCCACTCGT TCTCCGAGCC AGACAAGATC AACAAGCTCG GAGTGGAGGG TGAGGGAGAA 
CATCCTGTTG GAACAGCACG GTCATTTATA GACCGGCGGA AGTTCTTTGA AATGGCTGCT 
AAACCTTCCT TCTGCAGACC CATCTCAACC ATCCACAAGT CAGGCCAGCA GAGCTCCAGC 
ACCACCTCTG GCACTTTGGA GCGCGGAGAG GGCAAGGCCA GAGGGAGAGC CCACTCAGGC 
GAAACTGAGG AGGGCTGGAG GCCAGGCCTT GCCAATGAGA CTTCCAGTGA CCACCACCCA 
GACCCCCTCA GCCCCGCAGG CAGACAGGCC CTACTGGAGC AGCAGAGGCT GGGGACCTTC 
GCTGAGTACC AGGTCACCTG GAACATGCAG AGGAAGGCTT CAGACACAAA GCCCCAGGGG 
AGGTACCACT CTGCTGACAA CATCCTGGAC CAGGGTACAG AGGAGACGCC TGTCTGTGTC 
CATGAGAGGT CCAGATCGTC TCCCTCACAA GACTTCCACA CACAGAAGAT CCCTATGCCA 
TGGAGAGAGA CTGTTGAAAA CCCGTATCTG GATCACAGAC GGGACCAGCA AGGAGGCAGT 
TACACCGCCA GAGGGCAGAG CGAGAGCAGA GGGCAGCCAG CCCAGCTCCA CCACTTCCCA 
CAGCCTCCAC AACCGTCTAT TCCAAGACTG ACCGACACAG AGCCACACAG CAGCAGAGAC 
GTGGCCACCC CCACCCCCCT CCCTCTCCCC TCCGAACACA GATACAAACG TGACTCTGCG 
AACCCCGCCC ACAACCTCCC CGCTCTCCCC CCGTACCCCT CCAATCACAC CCACAGCTCT 
GTGTCTGAGC AACCACTACC ACCTCCAACG CCCGCTCCCA AGCCCCAGAA TACAGGCCTC 
ATCATCATGC CACATTGGCC TCTCCTAGGC CTGGAAACCT CAACCACATC CTCCTCCTAC 
GGACTGTCTC CCCAGGAATT CCTGCCTGGC CCTTGCACCC ATCAGGCTGA ACCATCATCC 
AGCAGCAGCT GCTCCTCCCA TGGCACAGAG CTGGATCCTG CCCCAAACCA AGCCTGCTCC 
TTGGGCTCCA CCCAGCTCCC CTCTCCCTCC CCTCGGAGAA CCACTGGACT GGGCACGGAG 
GAGGGAGCAG CTGATTCAAC ACTAGAGCCG CCACCCTCCC CCTCTTCCCA CTCTCCTTTT 
TTCTCCTACC AGCCTGCCAG TCTGACGGTT CCCTCCTCAG GTGGGACTAG TTCTCCCTCT 
CCTCATTTTG CTCCACAGAG GTTGACGGAC CAACCCCCTG TCTCTGTGTC TATGCAGGAT 
GAAGCCCAGA GCAGGCCAGA GAACAGGACC AACGCTGTGA TGGAGATGAG CAGTGTAGGG 
AAGAAGGTCC CTGTTAAAAT CGTCCATGCT GAGAGCACCA CAGAGAGGGA GAGCCGCCAG 
TACCTGCTGC ACAGCGAGAA AAATAGGGCC CCTGGAGTTT CCGAGGGGCC CGACCTTCCC 
CCGCCCCTAC CGACCAGCCT GCCCTCCCCT GAGCCGCAGC CCTACTCCCT GTTCCGTGCC 
TACACCCCCT ACACACGCCA TGGGCCCCAG AGTCCCCCCA GAGACCCAAC CCTCACTGTA 
GCCCCAGAAG AGGCCCTGTC CCCTGGGCAG TCTCAGACAA ACGGCCCCTC CGGTACCTCC 
ACCATGGGTC CCCAGCAGCC TCAGAAGAGT AACTCAGACG AGGATGTGAA GAGAGAGGAG 
CTGGCCAGAG ACATCATGGA CAAGGATAAG TCCCTGGTAG ACATCTTGGA CCAGAGTAAG 
ATGAAGACCA CCATGGATCT GATGGAGGGG ATCTTCCCCC AGGGGGAGCA GATCTTGGAT 
GGGGGGCACC AGAGGAGGAA AGTCTCCCCC AAACAGGGAT TGCCGCCCAG GGGAATGGAT 
GACCGGAGAG AGGAGGAGGG CATGTCTGCT GCCACAGGGT CCCTGGTGAC CAGCTCCTCT 
TACTACAGCA CATCTGCCCC CAAGGCTGAG CTGCTCAACA AGATGAAGGA CATGCAGGAG 
GACGAGCTGG AGGAGCTGGA GCAAGACTCG GAGGACGAAC TGGACATCAA CCTGGCCAGC 
AAGAAGCAAG AGCTGATTGA CAGCCTGGGT AAGAAGCTGC AGGTGCTACG CGAGGCCAGG 
GAGAGCCTTC AAGAGGATGT CCATGACAAC AACTCTCTGG GGGACGAGGT GGAAGCCGTG 
GTGCAGAAGG TCTGCAAGCC CAACGAGCTG GACAAGTTCA GGATGTTCGT GGGAGATCTG 
GACAAGGTGG TCAGTTTGCT GCTGTCCCTG TCTGGCCGAC TGGCCAGAGT GGAGAACGCT 
CTCAACAGTC TGGAGGAAGA CACTACACCA GAAGAGAAGC GTACCCTGTC AGAGAAGAGG 
AAGTTGTTGA TCAGACAACA CGAAGACGCC AAAGAGCTCA AGGAGAACCT TGACCGGCGG 
GAGCGCCTGG TTTACAGCAT CATGGCTGCT CACCTCAGCC ACGAGAGCCT GGCAGACTAC 
CAGCACTTTG TCAAGATGAA GTCAGCCCTC ATCATCGAGC AGCGCAAGCT GGAAGACAAG 
ATAAAACTGG GAGAGGAGCA GCTGAAATGT CTGCTGGATA GTTTATTGTT GGAGCAAAGG 
CTGCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021427064.1
CDS	2227..7218
Translation	MKMVDIASQTTMPSESETDKHVARNFLTKILRSSMRKNRFKGRNEPVSRPHSWHSSKFTEDHPEPTESHNEPPPAPPPVWQVKHEVSASTKDLSSCGDHDSNLRQLSSQFSSVGNMERVDRPSHPFQPGCLSPSRYHRSAEPLSGGGGSGGKRESAFSCLSSTSPPPEPALANTDTAATESSMFHKGVQTQTSEDGRPGEQRHSRYLQRPQGDGGSESPRTVPEEQPGSRYSSSGSGRSHIGPVWHVPERRRVAAPPSPPLRSDSFAVTKVYPAYTEGPGAPPHGQMKAQDRLAEAPENSSYRGRGNHISHDENGVDPRCSYNPPPCKKDFFHPNIAAGAPDYNHNQLSNPNKLSSVSSQDVRQSQSPFTCLPNHQRQYSDESTFYLQTRSAPHPPKPQSVGSYYHSLQELPTNCTNSRNHVRSSTTSLSTSTIDQNYDCGGHIRYYCITTKQPAQPETHVRQSKSEVWRSDMELAKGSNDRGSTSSSHKTNKVKYPPNPQPAYTNSKERNGNFQPANVLLYGHTTSNSLSSSAKPTTEENERKSEGQRPTEAQLRSSYSPSPPKDHKVGPLREDPWVSQENHKISSQKTPMLHSLAQESRSLAQEIRSPMLHSLAQESKSLVENNHPATAQPPPSNGGGEANHALQEALTDNTATGKLARRSDRYATTLRNEIQLKRAQLQKSRSAATLTCPSETEEPEEEETDPGGWKSTSSTSSDGSFSTSYKDHLKEAQARVLQATSFRRRDLEPPGSGSEAPLTKPNSHIVSRIGSRKRFPLNKRVHSFSEPDKINKLGVEGEGEHPVGTARSFIDRRKFFEMAAKPSFCRPISTIHKSGQQSSSTTSGTLERGEGKARGRAHSGETEEGWRPGLANETSSDHHPDPLSPAGRQALLEQQRLGTFAEYQVTWNMQRKASDTKPQGRYHSADNILDQGTEETPVCVHERSRSSPSQDFHTQKIPMPWRETVENPYLDHRRDQQGGSYTARGQSESRGQPAQLHHFPQPPQPSIPRLTDTEPHSSRDVATPTPLPLPSEHRYKRDSANPAHNLPALPPYPSNHTHSSVSEQPLPPPTPAPKPQNTGLIIMPHWPLLGLETSTTSSSYGLSPQEFLPGPCTHQAEPSSSSSCSSHGTELDPAPNQACSLGSTQLPSPSPRRTTGLGTEEGAADSTLEPPPSPSSHSPFFSYQPASLTVPSSGGTSSPSPHFAPQRLTDQPPVSVSMQDEAQSRPENRTNAVMEMSSVGKKVPVKIVHAESTTERESRQYLLHSEKNRAPGVSEGPDLPPPLPTSLPSPEPQPYSLFRAYTPYTRHGPQSPPRDPTLTVAPEEALSPGQSQTNGPSGTSTMGPQQPQKSNSDEDVKREELARDIMDKDKSLVDILDQSKMKTTMDLMEGIFPQGEQILDGGHQRRKVSPKQGLPPRGMDDRREEEGMSAATGSLVTSSSYYSTSAPKAELLNKMKDMQEDELEELEQDSEDELDINLASKKQELIDSLGKKLQVLREARESLQEDVHDNNSLGDEVEAVVQKVCKPNELDKFRMFVGDLDKVVSLLLSLSGRLARVENALNSLEEDTTPEEKRTLSEKRKLLIRQHEDAKELKENLDRRERLVYSIMAAHLSHESLADYQHFVKMKSALIIEQRKLEDKIKLGEEQLKCLLDSLLLEQRLLF

Target ORF information:

RefSeq Version	XM_021571389.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021571389.1

ORF Insert Sequence:

ATGAAAATGG TGGATATTGC CTCACAGACC ACCATGCCAT CAGAATCAGA AACTGACAAA 
CATGTGGCCA GGAACTTTCT CACCAAGATA TTACGAAGTT CTATGAGGAA GAACCGCTTC 
AAGGGGAGGA ATGAACCAGT CTCCCGTCCC CATTCATGGC ACTCCTCCAA GTTTACAGAA 
GACCACCCCG AACCCACAGA GAGTCACAAC GAGCCTCCAC CTGCACCCCC ACCTGTGTGG 
CAGGTCAAAC ATGAAGTCAG TGCATCCACA AAAGACCTTT CCAGCTGCGG GGACCACGAC 
TCGAATCTAC GCCAGTTATC CAGCCAGTTC AGCTCCGTGG GGAATATGGA GAGAGTAGAT 
CGCCCCTCAC ACCCCTTCCA ACCAGGATGC CTCTCCCCCA GCAGGTACCA CCGCAGCGCT 
GAGCCTCTCT CTGGGGGTGG AGGGTCTGGT GGGAAGAGAG AATCGGCCTT CAGTTGCCTC 
TCCTCCACCA GCCCTCCCCC GGAGCCTGCT CTGGCCAACA CTGACACTGC TGCAACTGAG 
AGCAGCATGT TCCATAAGGG GGTCCAGACT CAGACCAGTG AGGATGGAAG GCCGGGTGAG 
CAGCGCCACA GCCGGTACCT CCAGCGGCCC CAAGGGGACG GAGGCTCGGA GAGCCCCAGG 
ACAGTCCCAG AGGAGCAGCC TGGCTCCCGC TACTCCAGTT CAGGCTCTGG CAGATCGCAC 
ATAGGCCCTG TGTGGCATGT CCCAGAGAGG AGGAGGGTAG CAGCGCCCCC CTCTCCTCCC 
CTGCGCAGTG ACAGCTTTGC TGTCACCAAG GTGTACCCTG CCTACACAGA GGGGCCTGGA 
GCTCCACCAC ACGGGCAGAT GAAGGCCCAG GACAGGTTGG CAGAGGCCCC AGAGAACAGC 
AGCTACAGAG GCCGGGGCAA CCATATCTCT CACGATGAGA ACGGTGTAGA CCCTAGGTGC 
AGTTACAACC CTCCACCTTG CAAGAAAGAC TTCTTCCACC CAAACATAGC AGCAGGAGCA 
CCTGACTACA ACCACAACCA GCTCAGCAAC CCAAACAAAC TGTCCTCTGT GTCTAGCCAG 
GACGTGAGAC AGAGTCAGTC TCCCTTCACT TGCCTGCCCA ACCACCAGCG GCAGTATAGC 
GACGAAAGCA CTTTCTACCT GCAGACCAGA TCCGCCCCAC ATCCACCGAA GCCGCAGAGC 
GTCGGTAGCT ACTACCACAG CCTCCAGGAG CTCCCTACCA ACTGCACTAA CAGCAGGAAC 
CACGTGAGGT CCTCCACCAC GTCCCTGTCC ACCTCGACCA TCGACCAGAA CTATGACTGC 
GGAGGACACA TCCGGTACTA CTGCATCACA ACCAAGCAGC CAGCCCAGCC AGAGACTCAT 
GTTAGACAGA GCAAATCAGA GGTCTGGAGA TCTGACATGG AGCTAGCTAA GGGCTCCAAC 
GACAGGGGCT CTACAAGTTC CTCCCACAAG ACCAACAAGG TCAAGTATCC TCCTAATCCT 
CAGCCTGCTT ACACCAACAG CAAGGAGCGG AACGGAAATT TCCAACCGGC CAATGTTCTG 
CTTTACGGCC ACACCACCTC TAATTCCCTA TCCTCATCTG CTAAACCTAC CACTGAGGAG 
AATGAGAGGA AGAGTGAGGG CCAGAGACCT ACAGAGGCCC AGCTTAGAAG TTCTTACTCT 
CCCAGTCCGC CCAAGGACCA CAAGGTGGGA CCACTGAGAG AAGACCCTTG GGTCTCTCAG 
GAGAACCATA AGATCTCTTC TCAAAAGACA CCCATGCTCC ACAGTCTGGC TCAGGAGAGT 
AGAAGCCTAG CCCAGGAGAT TAGGAGCCCA ATGCTTCATT CTCTAGCCCA GGAGAGTAAG 
AGCCTGGTGG AGAACAATCA CCCTGCTACG GCACAACCAC CACCATCCAA CGGCGGGGGA 
GAAGCCAACC ACGCCCTACA GGAGGCTTTA ACGGACAACA CGGCAACGGG CAAACTGGCG 
AGACGCAGCG ACCGATACGC CACCACCCTC CGCAACGAGA TCCAGCTAAA GAGGGCCCAG 
CTGCAGAAAA GCCGGAGTGC TGCCACCCTG ACCTGCCCCA GCGAGACGGA GGAGCCAGAG 
GAAGAGGAGA CAGACCCGGG GGGGTGGAAG TCCACCTCCT CCACCTCCTC TGATGGCTCC 
TTTTCCACGT CCTACAAGGA CCACCTCAAA GAGGCGCAGG CGAGGGTCCT CCAGGCCACG 
TCCTTTAGGA GGAGAGACCT AGAACCTCCC GGGTCAGGGT CGGAGGCTCC GTTAACCAAA 
CCCAACTCAC ACATAGTCTC CCGCATTGGC AGCCGCAAGC GTTTCCCTCT GAACAAGAGG 
GTCCACTCGT TCTCCGAGCC AGACAAGATC AACAAGCTCG GAGTGGAGGG TGAGGGAGAA 
CATCCTGTTG GAACAGCACG GTCATTTATA GACCGGCGGA AGTTCTTTGA AATGGCTGCT 
AAACCTTCCT TCTGCAGACC CATCTCAACC ATCCACAAGT CAGGCCAGCA GAGCTCCAGC 
ACCACCTCTG GCACTTTGGA GCGCGGAGAG GGCAAGGCCA GAGGGAGAGC CCACTCAGGC 
GAAACTGAGG AGGGCTGGAG GCCAGGCCTT GCCAATGAGA CTTCCAGTGA CCACCACCCA 
GACCCCCTCA GCCCCGCAGG CAGACAGGCC CTACTGGAGC AGCAGAGGCT GGGGACCTTC 
GCTGAGTACC AGGTCACCTG GAACATGCAG AGGAAGGCTT CAGACACAAA GCCCCAGGGG 
AGGTACCACT CTGCTGACAA CATCCTGGAC CAGGGTACAG AGGAGACGCC TGTCTGTGTC 
CATGAGAGGT CCAGATCGTC TCCCTCACAA GACTTCCACA CACAGAAGAT CCCTATGCCA 
TGGAGAGAGA CTGTTGAAAA CCCGTATCTG GATCACAGAC GGGACCAGCA AGGAGGCAGT 
TACACCGCCA GAGGGCAGAG CGAGAGCAGA GGGCAGCCAG CCCAGCTCCA CCACTTCCCA 
CAGCCTCCAC AACCGTCTAT TCCAAGACTG ACCGACACAG AGCCACACAG CAGCAGAGAC 
GTGGCCACCC CCACCCCCCT CCCTCTCCCC TCCGAACACA GATACAAACG TGACTCTGCG 
AACCCCGCCC ACAACCTCCC CGCTCTCCCC CCGTACCCCT CCAATCACAC CCACAGCTCT 
GTGTCTGAGC AACCACTACC ACCTCCAACG CCCGCTCCCA AGCCCCAGAA TACAGGCCTC 
ATCATCATGC CACATTGGCC TCTCCTAGGC CTGGAAACCT CAACCACATC CTCCTCCTAC 
GGACTGTCTC CCCAGGAATT CCTGCCTGGC CCTTGCACCC ATCAGGCTGA ACCATCATCC 
AGCAGCAGCT GCTCCTCCCA TGGCACAGAG CTGGATCCTG CCCCAAACCA AGCCTGCTCC 
TTGGGCTCCA CCCAGCTCCC CTCTCCCTCC CCTCGGAGAA CCACTGGACT GGGCACGGAG 
GAGGGAGCAG CTGATTCAAC ACTAGAGCCG CCACCCTCCC CCTCTTCCCA CTCTCCTTTT 
TTCTCCTACC AGCCTGCCAG TCTGACGGTT CCCTCCTCAG GTGGGACTAG TTCTCCCTCT 
CCTCATTTTG CTCCACAGAG GTTGACGGAC CAACCCCCTG TCTCTGTGTC TATGCAGGAT 
GAAGCCCAGA GCAGGCCAGA GAACAGGACC AACGCTGTGA TGGAGATGAG CAGTGTAGGG 
AAGAAGGTCC CTGTTAAAAT CGTCCATGCT GAGAGCACCA CAGAGAGGGA GAGCCGCCAG 
TACCTGCTGC ACAGCGAGAA AAATAGGGCC CCTGGAGTTT CCGAGGGGCC CGACCTTCCC 
CCGCCCCTAC CGACCAGCCT GCCCTCCCCT GAGCCGCAGC CCTACTCCCT GTTCCGTGCC 
TACACCCCCT ACACACGCCA TGGGCCCCAG AGTCCCCCCA GAGACCCAAC CCTCACTGTA 
GCCCCAGAAG AGGCCCTGTC CCCTGGGCAG TCTCAGACAA ACGGCCCCTC CGGTACCTCC 
ACCATGGGTC CCCAGCAGCC TCAGAAGAGT AACTCAGACG AGGATGTGAA GAGAGAGGAG 
CTGGCCAGAG ACATCATGGA CAAGGATAAG TCCCTGGTAG ACATCTTGGA CCAGAGTAAG 
ATGAAGACCA CCATGGATCT GATGGAGGGG ATCTTCCCCC AGGGGGAGCA GATCTTGGAT 
GGGGGGCACC AGAGGAGGAA AGTCTCCCCC AAACAGGGAT TGCCGCCCAG GGGAATGGAT 
GACCGGAGAG AGGAGGAGGG CATGTCTGCT GCCACAGGGT CCCTGGTGAC CAGCTCCTCT 
TACTACAGCA CATCTGCCCC CAAGGCTGAG CTGCTCAACA AGATGAAGGA CATGCAGGAG 
GACGAGCTGG AGGAGCTGGA GCAAGACTCG GAGGACGAAC TGGACATCAA CCTGGCCAGC 
AAGAAGCAAG AGCTGATTGA CAGCCTGGGT AAGAAGCTGC AGGTGCTACG CGAGGCCAGG 
GAGAGCCTTC AAGAGGATGT CCATGACAAC AACTCTCTGG GGGACGAGGT GGAAGCCGTG 
GTGCAGAAGG TCTGCAAGCC CAACGAGCTG GACAAGTTCA GGATGTTCGT GGGAGATCTG 
GACAAGGTGG TCAGTTTGCT GCTGTCCCTG TCTGGCCGAC TGGCCAGAGT GGAGAACGCT 
CTCAACAGTC TGGAGGAAGA CACTACACCA GAAGAGAAGC GTACCCTGTC AGAGAAGAGG 
AAGTTGTTGA TCAGACAACA CGAAGACGCC AAAGAGCTCA AGGAGAACCT TGACCGGCGG 
GAGCGCCTGG TTTACAGCAT CATGGCTGCT CACCTCAGCC ACGAGAGCCT GGCAGACTAC 
CAGCACTTTG TCAAGATGAA GTCAGCCCTC ATCATCGAGC AGCGCAAGCT GGAAGACAAG 
ATAAAACTGG GAGAGGAGCA GCTGAAATGT CTGCTGGATA GTTTATTGTT GGAGCAAAGG 
CTGCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110495881 ( XM_021571390.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021427065.1 Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X6, mRNA.

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CloneID	OOf19775
Clone ID Related Accession (Same CDS sequence)	XM_021571389.1 , XM_021571390.1
Accession Version	XM_021571390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	protein Shroom2-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGG TGGATATTGC CTCACAGACC ACCATGCCAT CAGAATCAGA AACTGACAAA 
CATGTGGCCA GGAACTTTCT CACCAAGATA TTACGAAGTT CTATGAGGAA GAACCGCTTC 
AAGGGGAGGA ATGAACCAGT CTCCCGTCCC CATTCATGGC ACTCCTCCAA GTTTACAGAA 
GACCACCCCG AACCCACAGA GAGTCACAAC GAGCCTCCAC CTGCACCCCC ACCTGTGTGG 
CAGGTCAAAC ATGAAGTCAG TGCATCCACA AAAGACCTTT CCAGCTGCGG GGACCACGAC 
TCGAATCTAC GCCAGTTATC CAGCCAGTTC AGCTCCGTGG GGAATATGGA GAGAGTAGAT 
CGCCCCTCAC ACCCCTTCCA ACCAGGATGC CTCTCCCCCA GCAGGTACCA CCGCAGCGCT 
GAGCCTCTCT CTGGGGGTGG AGGGTCTGGT GGGAAGAGAG AATCGGCCTT CAGTTGCCTC 
TCCTCCACCA GCCCTCCCCC GGAGCCTGCT CTGGCCAACA CTGACACTGC TGCAACTGAG 
AGCAGCATGT TCCATAAGGG GGTCCAGACT CAGACCAGTG AGGATGGAAG GCCGGGTGAG 
CAGCGCCACA GCCGGTACCT CCAGCGGCCC CAAGGGGACG GAGGCTCGGA GAGCCCCAGG 
ACAGTCCCAG AGGAGCAGCC TGGCTCCCGC TACTCCAGTT CAGGCTCTGG CAGATCGCAC 
ATAGGCCCTG TGTGGCATGT CCCAGAGAGG AGGAGGGTAG CAGCGCCCCC CTCTCCTCCC 
CTGCGCAGTG ACAGCTTTGC TGTCACCAAG GTGTACCCTG CCTACACAGA GGGGCCTGGA 
GCTCCACCAC ACGGGCAGAT GAAGGCCCAG GACAGGTTGG CAGAGGCCCC AGAGAACAGC 
AGCTACAGAG GCCGGGGCAA CCATATCTCT CACGATGAGA ACGGTGTAGA CCCTAGGTGC 
AGTTACAACC CTCCACCTTG CAAGAAAGAC TTCTTCCACC CAAACATAGC AGCAGGAGCA 
CCTGACTACA ACCACAACCA GCTCAGCAAC CCAAACAAAC TGTCCTCTGT GTCTAGCCAG 
GACGTGAGAC AGAGTCAGTC TCCCTTCACT TGCCTGCCCA ACCACCAGCG GCAGTATAGC 
GACGAAAGCA CTTTCTACCT GCAGACCAGA TCCGCCCCAC ATCCACCGAA GCCGCAGAGC 
GTCGGTAGCT ACTACCACAG CCTCCAGGAG CTCCCTACCA ACTGCACTAA CAGCAGGAAC 
CACGTGAGGT CCTCCACCAC GTCCCTGTCC ACCTCGACCA TCGACCAGAA CTATGACTGC 
GGAGGACACA TCCGGTACTA CTGCATCACA ACCAAGCAGC CAGCCCAGCC AGAGACTCAT 
GTTAGACAGA GCAAATCAGA GGTCTGGAGA TCTGACATGG AGCTAGCTAA GGGCTCCAAC 
GACAGGGGCT CTACAAGTTC CTCCCACAAG ACCAACAAGG TCAAGTATCC TCCTAATCCT 
CAGCCTGCTT ACACCAACAG CAAGGAGCGG AACGGAAATT TCCAACCGGC CAATGTTCTG 
CTTTACGGCC ACACCACCTC TAATTCCCTA TCCTCATCTG CTAAACCTAC CACTGAGGAG 
AATGAGAGGA AGAGTGAGGG CCAGAGACCT ACAGAGGCCC AGCTTAGAAG TTCTTACTCT 
CCCAGTCCGC CCAAGGACCA CAAGGTGGGA CCACTGAGAG AAGACCCTTG GGTCTCTCAG 
GAGAACCATA AGATCTCTTC TCAAAAGACA CCCATGCTCC ACAGTCTGGC TCAGGAGAGT 
AGAAGCCTAG CCCAGGAGAT TAGGAGCCCA ATGCTTCATT CTCTAGCCCA GGAGAGTAAG 
AGCCTGGTGG AGAACAATCA CCCTGCTACG GCACAACCAC CACCATCCAA CGGCGGGGGA 
GAAGCCAACC ACGCCCTACA GGAGGCTTTA ACGGACAACA CGGCAACGGG CAAACTGGCG 
AGACGCAGCG ACCGATACGC CACCACCCTC CGCAACGAGA TCCAGCTAAA GAGGGCCCAG 
CTGCAGAAAA GCCGGAGTGC TGCCACCCTG ACCTGCCCCA GCGAGACGGA GGAGCCAGAG 
GAAGAGGAGA CAGACCCGGG GGGGTGGAAG TCCACCTCCT CCACCTCCTC TGATGGCTCC 
TTTTCCACGT CCTACAAGGA CCACCTCAAA GAGGCGCAGG CGAGGGTCCT CCAGGCCACG 
TCCTTTAGGA GGAGAGACCT AGAACCTCCC GGGTCAGGGT CGGAGGCTCC GTTAACCAAA 
CCCAACTCAC ACATAGTCTC CCGCATTGGC AGCCGCAAGC GTTTCCCTCT GAACAAGAGG 
GTCCACTCGT TCTCCGAGCC AGACAAGATC AACAAGCTCG GAGTGGAGGG TGAGGGAGAA 
CATCCTGTTG GAACAGCACG GTCATTTATA GACCGGCGGA AGTTCTTTGA AATGGCTGCT 
AAACCTTCCT TCTGCAGACC CATCTCAACC ATCCACAAGT CAGGCCAGCA GAGCTCCAGC 
ACCACCTCTG GCACTTTGGA GCGCGGAGAG GGCAAGGCCA GAGGGAGAGC CCACTCAGGC 
GAAACTGAGG AGGGCTGGAG GCCAGGCCTT GCCAATGAGA CTTCCAGTGA CCACCACCCA 
GACCCCCTCA GCCCCGCAGG CAGACAGGCC CTACTGGAGC AGCAGAGGCT GGGGACCTTC 
GCTGAGTACC AGGTCACCTG GAACATGCAG AGGAAGGCTT CAGACACAAA GCCCCAGGGG 
AGGTACCACT CTGCTGACAA CATCCTGGAC CAGGGTACAG AGGAGACGCC TGTCTGTGTC 
CATGAGAGGT CCAGATCGTC TCCCTCACAA GACTTCCACA CACAGAAGAT CCCTATGCCA 
TGGAGAGAGA CTGTTGAAAA CCCGTATCTG GATCACAGAC GGGACCAGCA AGGAGGCAGT 
TACACCGCCA GAGGGCAGAG CGAGAGCAGA GGGCAGCCAG CCCAGCTCCA CCACTTCCCA 
CAGCCTCCAC AACCGTCTAT TCCAAGACTG ACCGACACAG AGCCACACAG CAGCAGAGAC 
GTGGCCACCC CCACCCCCCT CCCTCTCCCC TCCGAACACA GATACAAACG TGACTCTGCG 
AACCCCGCCC ACAACCTCCC CGCTCTCCCC CCGTACCCCT CCAATCACAC CCACAGCTCT 
GTGTCTGAGC AACCACTACC ACCTCCAACG CCCGCTCCCA AGCCCCAGAA TACAGGCCTC 
ATCATCATGC CACATTGGCC TCTCCTAGGC CTGGAAACCT CAACCACATC CTCCTCCTAC 
GGACTGTCTC CCCAGGAATT CCTGCCTGGC CCTTGCACCC ATCAGGCTGA ACCATCATCC 
AGCAGCAGCT GCTCCTCCCA TGGCACAGAG CTGGATCCTG CCCCAAACCA AGCCTGCTCC 
TTGGGCTCCA CCCAGCTCCC CTCTCCCTCC CCTCGGAGAA CCACTGGACT GGGCACGGAG 
GAGGGAGCAG CTGATTCAAC ACTAGAGCCG CCACCCTCCC CCTCTTCCCA CTCTCCTTTT 
TTCTCCTACC AGCCTGCCAG TCTGACGGTT CCCTCCTCAG GTGGGACTAG TTCTCCCTCT 
CCTCATTTTG CTCCACAGAG GTTGACGGAC CAACCCCCTG TCTCTGTGTC TATGCAGGAT 
GAAGCCCAGA GCAGGCCAGA GAACAGGACC AACGCTGTGA TGGAGATGAG CAGTGTAGGG 
AAGAAGGTCC CTGTTAAAAT CGTCCATGCT GAGAGCACCA CAGAGAGGGA GAGCCGCCAG 
TACCTGCTGC ACAGCGAGAA AAATAGGGCC CCTGGAGTTT CCGAGGGGCC CGACCTTCCC 
CCGCCCCTAC CGACCAGCCT GCCCTCCCCT GAGCCGCAGC CCTACTCCCT GTTCCGTGCC 
TACACCCCCT ACACACGCCA TGGGCCCCAG AGTCCCCCCA GAGACCCAAC CCTCACTGTA 
GCCCCAGAAG AGGCCCTGTC CCCTGGGCAG TCTCAGACAA ACGGCCCCTC CGGTACCTCC 
ACCATGGGTC CCCAGCAGCC TCAGAAGAGT AACTCAGACG AGGATGTGAA GAGAGAGGAG 
CTGGCCAGAG ACATCATGGA CAAGGATAAG TCCCTGGTAG ACATCTTGGA CCAGAGTAAG 
ATGAAGACCA CCATGGATCT GATGGAGGGG ATCTTCCCCC AGGGGGAGCA GATCTTGGAT 
GGGGGGCACC AGAGGAGGAA AGTCTCCCCC AAACAGGGAT TGCCGCCCAG GGGAATGGAT 
GACCGGAGAG AGGAGGAGGG CATGTCTGCT GCCACAGGGT CCCTGGTGAC CAGCTCCTCT 
TACTACAGCA CATCTGCCCC CAAGGCTGAG CTGCTCAACA AGATGAAGGA CATGCAGGAG 
GACGAGCTGG AGGAGCTGGA GCAAGACTCG GAGGACGAAC TGGACATCAA CCTGGCCAGC 
AAGAAGCAAG AGCTGATTGA CAGCCTGGGT AAGAAGCTGC AGGTGCTACG CGAGGCCAGG 
GAGAGCCTTC AAGAGGATGT CCATGACAAC AACTCTCTGG GGGACGAGGT GGAAGCCGTG 
GTGCAGAAGG TCTGCAAGCC CAACGAGCTG GACAAGTTCA GGATGTTCGT GGGAGATCTG 
GACAAGGTGG TCAGTTTGCT GCTGTCCCTG TCTGGCCGAC TGGCCAGAGT GGAGAACGCT 
CTCAACAGTC TGGAGGAAGA CACTACACCA GAAGAGAAGC GTACCCTGTC AGAGAAGAGG 
AAGTTGTTGA TCAGACAACA CGAAGACGCC AAAGAGCTCA AGGAGAACCT TGACCGGCGG 
GAGCGCCTGG TTTACAGCAT CATGGCTGCT CACCTCAGCC ACGAGAGCCT GGCAGACTAC 
CAGCACTTTG TCAAGATGAA GTCAGCCCTC ATCATCGAGC AGCGCAAGCT GGAAGACAAG 
ATAAAACTGG GAGAGGAGCA GCTGAAATGT CTGCTGGATA GTTTATTGTT GGAGCAAAGG 
CTGCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021427065.1
CDS	198..5189
Translation	MKMVDIASQTTMPSESETDKHVARNFLTKILRSSMRKNRFKGRNEPVSRPHSWHSSKFTEDHPEPTESHNEPPPAPPPVWQVKHEVSASTKDLSSCGDHDSNLRQLSSQFSSVGNMERVDRPSHPFQPGCLSPSRYHRSAEPLSGGGGSGGKRESAFSCLSSTSPPPEPALANTDTAATESSMFHKGVQTQTSEDGRPGEQRHSRYLQRPQGDGGSESPRTVPEEQPGSRYSSSGSGRSHIGPVWHVPERRRVAAPPSPPLRSDSFAVTKVYPAYTEGPGAPPHGQMKAQDRLAEAPENSSYRGRGNHISHDENGVDPRCSYNPPPCKKDFFHPNIAAGAPDYNHNQLSNPNKLSSVSSQDVRQSQSPFTCLPNHQRQYSDESTFYLQTRSAPHPPKPQSVGSYYHSLQELPTNCTNSRNHVRSSTTSLSTSTIDQNYDCGGHIRYYCITTKQPAQPETHVRQSKSEVWRSDMELAKGSNDRGSTSSSHKTNKVKYPPNPQPAYTNSKERNGNFQPANVLLYGHTTSNSLSSSAKPTTEENERKSEGQRPTEAQLRSSYSPSPPKDHKVGPLREDPWVSQENHKISSQKTPMLHSLAQESRSLAQEIRSPMLHSLAQESKSLVENNHPATAQPPPSNGGGEANHALQEALTDNTATGKLARRSDRYATTLRNEIQLKRAQLQKSRSAATLTCPSETEEPEEEETDPGGWKSTSSTSSDGSFSTSYKDHLKEAQARVLQATSFRRRDLEPPGSGSEAPLTKPNSHIVSRIGSRKRFPLNKRVHSFSEPDKINKLGVEGEGEHPVGTARSFIDRRKFFEMAAKPSFCRPISTIHKSGQQSSSTTSGTLERGEGKARGRAHSGETEEGWRPGLANETSSDHHPDPLSPAGRQALLEQQRLGTFAEYQVTWNMQRKASDTKPQGRYHSADNILDQGTEETPVCVHERSRSSPSQDFHTQKIPMPWRETVENPYLDHRRDQQGGSYTARGQSESRGQPAQLHHFPQPPQPSIPRLTDTEPHSSRDVATPTPLPLPSEHRYKRDSANPAHNLPALPPYPSNHTHSSVSEQPLPPPTPAPKPQNTGLIIMPHWPLLGLETSTTSSSYGLSPQEFLPGPCTHQAEPSSSSSCSSHGTELDPAPNQACSLGSTQLPSPSPRRTTGLGTEEGAADSTLEPPPSPSSHSPFFSYQPASLTVPSSGGTSSPSPHFAPQRLTDQPPVSVSMQDEAQSRPENRTNAVMEMSSVGKKVPVKIVHAESTTERESRQYLLHSEKNRAPGVSEGPDLPPPLPTSLPSPEPQPYSLFRAYTPYTRHGPQSPPRDPTLTVAPEEALSPGQSQTNGPSGTSTMGPQQPQKSNSDEDVKREELARDIMDKDKSLVDILDQSKMKTTMDLMEGIFPQGEQILDGGHQRRKVSPKQGLPPRGMDDRREEEGMSAATGSLVTSSSYYSTSAPKAELLNKMKDMQEDELEELEQDSEDELDINLASKKQELIDSLGKKLQVLREARESLQEDVHDNNSLGDEVEAVVQKVCKPNELDKFRMFVGDLDKVVSLLLSLSGRLARVENALNSLEEDTTPEEKRTLSEKRKLLIRQHEDAKELKENLDRRERLVYSIMAAHLSHESLADYQHFVKMKSALIIEQRKLEDKIKLGEEQLKCLLDSLLLEQRLLF

Target ORF information:

RefSeq Version	XM_021571390.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021571390.1

ORF Insert Sequence:

ATGAAAATGG TGGATATTGC CTCACAGACC ACCATGCCAT CAGAATCAGA AACTGACAAA 
CATGTGGCCA GGAACTTTCT CACCAAGATA TTACGAAGTT CTATGAGGAA GAACCGCTTC 
AAGGGGAGGA ATGAACCAGT CTCCCGTCCC CATTCATGGC ACTCCTCCAA GTTTACAGAA 
GACCACCCCG AACCCACAGA GAGTCACAAC GAGCCTCCAC CTGCACCCCC ACCTGTGTGG 
CAGGTCAAAC ATGAAGTCAG TGCATCCACA AAAGACCTTT CCAGCTGCGG GGACCACGAC 
TCGAATCTAC GCCAGTTATC CAGCCAGTTC AGCTCCGTGG GGAATATGGA GAGAGTAGAT 
CGCCCCTCAC ACCCCTTCCA ACCAGGATGC CTCTCCCCCA GCAGGTACCA CCGCAGCGCT 
GAGCCTCTCT CTGGGGGTGG AGGGTCTGGT GGGAAGAGAG AATCGGCCTT CAGTTGCCTC 
TCCTCCACCA GCCCTCCCCC GGAGCCTGCT CTGGCCAACA CTGACACTGC TGCAACTGAG 
AGCAGCATGT TCCATAAGGG GGTCCAGACT CAGACCAGTG AGGATGGAAG GCCGGGTGAG 
CAGCGCCACA GCCGGTACCT CCAGCGGCCC CAAGGGGACG GAGGCTCGGA GAGCCCCAGG 
ACAGTCCCAG AGGAGCAGCC TGGCTCCCGC TACTCCAGTT CAGGCTCTGG CAGATCGCAC 
ATAGGCCCTG TGTGGCATGT CCCAGAGAGG AGGAGGGTAG CAGCGCCCCC CTCTCCTCCC 
CTGCGCAGTG ACAGCTTTGC TGTCACCAAG GTGTACCCTG CCTACACAGA GGGGCCTGGA 
GCTCCACCAC ACGGGCAGAT GAAGGCCCAG GACAGGTTGG CAGAGGCCCC AGAGAACAGC 
AGCTACAGAG GCCGGGGCAA CCATATCTCT CACGATGAGA ACGGTGTAGA CCCTAGGTGC 
AGTTACAACC CTCCACCTTG CAAGAAAGAC TTCTTCCACC CAAACATAGC AGCAGGAGCA 
CCTGACTACA ACCACAACCA GCTCAGCAAC CCAAACAAAC TGTCCTCTGT GTCTAGCCAG 
GACGTGAGAC AGAGTCAGTC TCCCTTCACT TGCCTGCCCA ACCACCAGCG GCAGTATAGC 
GACGAAAGCA CTTTCTACCT GCAGACCAGA TCCGCCCCAC ATCCACCGAA GCCGCAGAGC 
GTCGGTAGCT ACTACCACAG CCTCCAGGAG CTCCCTACCA ACTGCACTAA CAGCAGGAAC 
CACGTGAGGT CCTCCACCAC GTCCCTGTCC ACCTCGACCA TCGACCAGAA CTATGACTGC 
GGAGGACACA TCCGGTACTA CTGCATCACA ACCAAGCAGC CAGCCCAGCC AGAGACTCAT 
GTTAGACAGA GCAAATCAGA GGTCTGGAGA TCTGACATGG AGCTAGCTAA GGGCTCCAAC 
GACAGGGGCT CTACAAGTTC CTCCCACAAG ACCAACAAGG TCAAGTATCC TCCTAATCCT 
CAGCCTGCTT ACACCAACAG CAAGGAGCGG AACGGAAATT TCCAACCGGC CAATGTTCTG 
CTTTACGGCC ACACCACCTC TAATTCCCTA TCCTCATCTG CTAAACCTAC CACTGAGGAG 
AATGAGAGGA AGAGTGAGGG CCAGAGACCT ACAGAGGCCC AGCTTAGAAG TTCTTACTCT 
CCCAGTCCGC CCAAGGACCA CAAGGTGGGA CCACTGAGAG AAGACCCTTG GGTCTCTCAG 
GAGAACCATA AGATCTCTTC TCAAAAGACA CCCATGCTCC ACAGTCTGGC TCAGGAGAGT 
AGAAGCCTAG CCCAGGAGAT TAGGAGCCCA ATGCTTCATT CTCTAGCCCA GGAGAGTAAG 
AGCCTGGTGG AGAACAATCA CCCTGCTACG GCACAACCAC CACCATCCAA CGGCGGGGGA 
GAAGCCAACC ACGCCCTACA GGAGGCTTTA ACGGACAACA CGGCAACGGG CAAACTGGCG 
AGACGCAGCG ACCGATACGC CACCACCCTC CGCAACGAGA TCCAGCTAAA GAGGGCCCAG 
CTGCAGAAAA GCCGGAGTGC TGCCACCCTG ACCTGCCCCA GCGAGACGGA GGAGCCAGAG 
GAAGAGGAGA CAGACCCGGG GGGGTGGAAG TCCACCTCCT CCACCTCCTC TGATGGCTCC 
TTTTCCACGT CCTACAAGGA CCACCTCAAA GAGGCGCAGG CGAGGGTCCT CCAGGCCACG 
TCCTTTAGGA GGAGAGACCT AGAACCTCCC GGGTCAGGGT CGGAGGCTCC GTTAACCAAA 
CCCAACTCAC ACATAGTCTC CCGCATTGGC AGCCGCAAGC GTTTCCCTCT GAACAAGAGG 
GTCCACTCGT TCTCCGAGCC AGACAAGATC AACAAGCTCG GAGTGGAGGG TGAGGGAGAA 
CATCCTGTTG GAACAGCACG GTCATTTATA GACCGGCGGA AGTTCTTTGA AATGGCTGCT 
AAACCTTCCT TCTGCAGACC CATCTCAACC ATCCACAAGT CAGGCCAGCA GAGCTCCAGC 
ACCACCTCTG GCACTTTGGA GCGCGGAGAG GGCAAGGCCA GAGGGAGAGC CCACTCAGGC 
GAAACTGAGG AGGGCTGGAG GCCAGGCCTT GCCAATGAGA CTTCCAGTGA CCACCACCCA 
GACCCCCTCA GCCCCGCAGG CAGACAGGCC CTACTGGAGC AGCAGAGGCT GGGGACCTTC 
GCTGAGTACC AGGTCACCTG GAACATGCAG AGGAAGGCTT CAGACACAAA GCCCCAGGGG 
AGGTACCACT CTGCTGACAA CATCCTGGAC CAGGGTACAG AGGAGACGCC TGTCTGTGTC 
CATGAGAGGT CCAGATCGTC TCCCTCACAA GACTTCCACA CACAGAAGAT CCCTATGCCA 
TGGAGAGAGA CTGTTGAAAA CCCGTATCTG GATCACAGAC GGGACCAGCA AGGAGGCAGT 
TACACCGCCA GAGGGCAGAG CGAGAGCAGA GGGCAGCCAG CCCAGCTCCA CCACTTCCCA 
CAGCCTCCAC AACCGTCTAT TCCAAGACTG ACCGACACAG AGCCACACAG CAGCAGAGAC 
GTGGCCACCC CCACCCCCCT CCCTCTCCCC TCCGAACACA GATACAAACG TGACTCTGCG 
AACCCCGCCC ACAACCTCCC CGCTCTCCCC CCGTACCCCT CCAATCACAC CCACAGCTCT 
GTGTCTGAGC AACCACTACC ACCTCCAACG CCCGCTCCCA AGCCCCAGAA TACAGGCCTC 
ATCATCATGC CACATTGGCC TCTCCTAGGC CTGGAAACCT CAACCACATC CTCCTCCTAC 
GGACTGTCTC CCCAGGAATT CCTGCCTGGC CCTTGCACCC ATCAGGCTGA ACCATCATCC 
AGCAGCAGCT GCTCCTCCCA TGGCACAGAG CTGGATCCTG CCCCAAACCA AGCCTGCTCC 
TTGGGCTCCA CCCAGCTCCC CTCTCCCTCC CCTCGGAGAA CCACTGGACT GGGCACGGAG 
GAGGGAGCAG CTGATTCAAC ACTAGAGCCG CCACCCTCCC CCTCTTCCCA CTCTCCTTTT 
TTCTCCTACC AGCCTGCCAG TCTGACGGTT CCCTCCTCAG GTGGGACTAG TTCTCCCTCT 
CCTCATTTTG CTCCACAGAG GTTGACGGAC CAACCCCCTG TCTCTGTGTC TATGCAGGAT 
GAAGCCCAGA GCAGGCCAGA GAACAGGACC AACGCTGTGA TGGAGATGAG CAGTGTAGGG 
AAGAAGGTCC CTGTTAAAAT CGTCCATGCT GAGAGCACCA CAGAGAGGGA GAGCCGCCAG 
TACCTGCTGC ACAGCGAGAA AAATAGGGCC CCTGGAGTTT CCGAGGGGCC CGACCTTCCC 
CCGCCCCTAC CGACCAGCCT GCCCTCCCCT GAGCCGCAGC CCTACTCCCT GTTCCGTGCC 
TACACCCCCT ACACACGCCA TGGGCCCCAG AGTCCCCCCA GAGACCCAAC CCTCACTGTA 
GCCCCAGAAG AGGCCCTGTC CCCTGGGCAG TCTCAGACAA ACGGCCCCTC CGGTACCTCC 
ACCATGGGTC CCCAGCAGCC TCAGAAGAGT AACTCAGACG AGGATGTGAA GAGAGAGGAG 
CTGGCCAGAG ACATCATGGA CAAGGATAAG TCCCTGGTAG ACATCTTGGA CCAGAGTAAG 
ATGAAGACCA CCATGGATCT GATGGAGGGG ATCTTCCCCC AGGGGGAGCA GATCTTGGAT 
GGGGGGCACC AGAGGAGGAA AGTCTCCCCC AAACAGGGAT TGCCGCCCAG GGGAATGGAT 
GACCGGAGAG AGGAGGAGGG CATGTCTGCT GCCACAGGGT CCCTGGTGAC CAGCTCCTCT 
TACTACAGCA CATCTGCCCC CAAGGCTGAG CTGCTCAACA AGATGAAGGA CATGCAGGAG 
GACGAGCTGG AGGAGCTGGA GCAAGACTCG GAGGACGAAC TGGACATCAA CCTGGCCAGC 
AAGAAGCAAG AGCTGATTGA CAGCCTGGGT AAGAAGCTGC AGGTGCTACG CGAGGCCAGG 
GAGAGCCTTC AAGAGGATGT CCATGACAAC AACTCTCTGG GGGACGAGGT GGAAGCCGTG 
GTGCAGAAGG TCTGCAAGCC CAACGAGCTG GACAAGTTCA GGATGTTCGT GGGAGATCTG 
GACAAGGTGG TCAGTTTGCT GCTGTCCCTG TCTGGCCGAC TGGCCAGAGT GGAGAACGCT 
CTCAACAGTC TGGAGGAAGA CACTACACCA GAAGAGAAGC GTACCCTGTC AGAGAAGAGG 
AAGTTGTTGA TCAGACAACA CGAAGACGCC AAAGAGCTCA AGGAGAACCT TGACCGGCGG 
GAGCGCCTGG TTTACAGCAT CATGGCTGCT CACCTCAGCC ACGAGAGCCT GGCAGACTAC 
CAGCACTTTG TCAAGATGAA GTCAGCCCTC ATCATCGAGC AGCGCAAGCT GGAAGACAAG 
ATAAAACTGG GAGAGGAGCA GCTGAAATGT CTGCTGGATA GTTTATTGTT GGAGCAAAGG 
CTGCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110495881 ( XM_021571391.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021427066.1 Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X7, mRNA.

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CloneID	OOf19776
Clone ID Related Accession (Same CDS sequence)	XM_021571391.1 , XM_021571392.1
Accession Version	XM_021571391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	protein Shroom2-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG TAGATCGCCC CTCACACCCC TTCCAACCAG GATGCCTCTC CCCCAGCAGG 
TACCACCGCA GCGCTGAGCC TCTCTCTGGG GGTGGAGGGT CTGGTGGGAA GAGAGAATCG 
GCCTTCAGTT GCCTCTCCTC CACCAGCCCT CCCCCGGAGC CTGCTCTGGC CAACACTGAC 
ACTGCTGCAA CTGAGAGCAG CATGTTCCAT AAGGGGGTCC AGACTCAGAC CAGTGAGGAT 
GGAAGGCCGG GTGAGCAGCG CCACAGCCGG TACCTCCAGC GGCCCCAAGG GGACGGAGGC 
TCGGAGAGCC CCAGGACAGT CCCAGAGGAG CAGCCTGGCT CCCGCTACTC CAGTTCAGGC 
TCTGGCAGAT CGCACATAGG CCCTGTGTGG CATGTCCCAG AGAGGAGGAG GGTAGCAGCG 
CCCCCCTCTC CTCCCCTGCG CAGTGACAGC TTTGCTGTCA CCAAGGTGTA CCCTGCCTAC 
ACAGAGGGGC CTGGAGCTCC ACCACACGGG CAGATGAAGG CCCAGGACAG GTTGGCAGAG 
GCCCCAGAGA ACAGCAGCTA CAGAGGCCGG GGCAACCATA TCTCTCACGA TGAGAACGGT 
GTAGACCCTA GGTGCAGTTA CAACCCTCCA CCTTGCAAGA AAGACTTCTT CCACCCAAAC 
ATAGCAGCAG GAGCACCTGA CTACAACCAC AACCAGCTCA GCAACCCAAA CAAACTGTCC 
TCTGTGTCTA GCCAGGACGT GAGACAGAGT CAGTCTCCCT TCACTTGCCT GCCCAACCAC 
CAGCGGCAGT ATAGCGACGA AAGCACTTTC TACCTGCAGA CCAGATCCGC CCCACATCCA 
CCGAAGCCGC AGAGCGTCGG TAGCTACTAC CACAGCCTCC AGGAGCTCCC TACCAACTGC 
ACTAACAGCA GGAACCACGT GAGGTCCTCC ACCACGTCCC TGTCCACCTC GACCATCGAC 
CAGAACTATG ACTGCGGAGG ACACATCCGG TACTACTGCA TCACAACCAA GCAGCCAGCC 
CAGCCAGAGA CTCATGTTAG ACAGAGCAAA TCAGAGGTCT GGAGATCTGA CATGGAGCTA 
GCTAAGGGCT CCAACGACAG GGGCTCTACA AGTTCCTCCC ACAAGACCAA CAAGGTCAAG 
TATCCTCCTA ATCCTCAGCC TGCTTACACC AACAGCAAGG AGCGGAACGG AAATTTCCAA 
CCGGCCAATG TTCTGCTTTA CGGCCACACC ACCTCTAATT CCCTATCCTC ATCTGCTAAA 
CCTACCACTG AGGAGAATGA GAGGAAGAGT GAGGGCCAGA GACCTACAGA GGCCCAGCTT 
AGAAGTTCTT ACTCTCCCAG TCCGCCCAAG GACCACAAGG TGGGACCACT GAGAGAAGAC 
CCTTGGGTCT CTCAGGAGAA CCATAAGATC TCTTCTCAAA AGACACCCAT GCTCCACAGT 
CTGGCTCAGG AGAGTAGAAG CCTAGCCCAG GAGATTAGGA GCCCAATGCT TCATTCTCTA 
GCCCAGGAGA GTAAGAGCCT GGTGGAGAAC AATCACCCTG CTACGGCACA ACCACCACCA 
TCCAACGGCG GGGGAGAAGC CAACCACGCC CTACAGGAGG CTTTAACGGA CAACACGGCA 
ACGGGCAAAC TGGCGAGACG CAGCGACCGA TACGCCACCA CCCTCCGCAA CGAGATCCAG 
CTAAAGAGGG CCCAGCTGCA GAAAAGCCGG AGTGCTGCCA CCCTGACCTG CCCCAGCGAG 
ACGGAGGAGC CAGAGGAAGA GGAGACAGAC CCGGGGGGGT GGAAGTCCAC CTCCTCCACC 
TCCTCTGATG GCTCCTTTTC CACGTCCTAC AAGGACCACC TCAAAGAGGC GCAGGCGAGG 
GTCCTCCAGG CCACGTCCTT TAGGAGGAGA GACCTAGAAC CTCCCGGGTC AGGGTCGGAG 
GCTCCGTTAA CCAAACCCAA CTCACACATA GTCTCCCGCA TTGGCAGCCG CAAGCGTTTC 
CCTCTGAACA AGAGGGTCCA CTCGTTCTCC GAGCCAGACA AGATCAACAA GCTCGGAGTG 
GAGGGTGAGG GAGAACATCC TGTTGGAACA GCACGGTCAT TTATAGACCG GCGGAAGTTC 
TTTGAAATGG CTGCTAAACC TTCCTTCTGC AGACCCATCT CAACCATCCA CAAGTCAGGC 
CAGCAGAGCT CCAGCACCAC CTCTGGCACT TTGGAGCGCG GAGAGGGCAA GGCCAGAGGG 
AGAGCCCACT CAGGCGAAAC TGAGGAGGGC TGGAGGCCAG GCCTTGCCAA TGAGACTTCC 
AGTGACCACC ACCCAGACCC CCTCAGCCCC GCAGGCAGAC AGGCCCTACT GGAGCAGCAG 
AGGCTGGGGA CCTTCGCTGA GTACCAGGTC ACCTGGAACA TGCAGAGGAA GGCTTCAGAC 
ACAAAGCCCC AGGGGAGGTA CCACTCTGCT GACAACATCC TGGACCAGGG TACAGAGGAG 
ACGCCTGTCT GTGTCCATGA GAGGTCCAGA TCGTCTCCCT CACAAGACTT CCACACACAG 
AAGATCCCTA TGCCATGGAG AGAGACTGTT GAAAACCCGT ATCTGGATCA CAGACGGGAC 
CAGCAAGGAG GCAGTTACAC CGCCAGAGGG CAGAGCGAGA GCAGAGGGCA GCCAGCCCAG 
CTCCACCACT TCCCACAGCC TCCACAACCG TCTATTCCAA GACTGACCGA CACAGAGCCA 
CACAGCAGCA GAGACGTGGC CACCCCCACC CCCCTCCCTC TCCCCTCCGA ACACAGATAC 
AAACGTGACT CTGCGAACCC CGCCCACAAC CTCCCCGCTC TCCCCCCGTA CCCCTCCAAT 
CACACCCACA GCTCTGTGTC TGAGCAACCA CTACCACCTC CAACGCCCGC TCCCAAGCCC 
CAGAATACAG GCCTCATCAT CATGCCACAT TGGCCTCTCC TAGGCCTGGA AACCTCAACC 
ACATCCTCCT CCTACGGACT GTCTCCCCAG GAATTCCTGC CTGGCCCTTG CACCCATCAG 
GCTGAACCAT CATCCAGCAG CAGCTGCTCC TCCCATGGCA CAGAGCTGGA TCCTGCCCCA 
AACCAAGCCT GCTCCTTGGG CTCCACCCAG CTCCCCTCTC CCTCCCCTCG GAGAACCACT 
GGACTGGGCA CGGAGGAGGG AGCAGCTGAT TCAACACTAG AGCCGCCACC CTCCCCCTCT 
TCCCACTCTC CTTTTTTCTC CTACCAGCCT GCCAGTCTGA CGGTTCCCTC CTCAGGTGGG 
ACTAGTTCTC CCTCTCCTCA TTTTGCTCCA CAGAGGTTGA CGGACCAACC CCCTGTCTCT 
GTGTCTATGC AGGATGAAGC CCAGAGCAGG CCAGAGAACA GGACCAACGC TGTGATGGAG 
ATGAGCAGTG TAGGGAAGAA GGTCCCTGTT AAAATCGTCC ATGCTGAGAG CACCACAGAG 
AGGGAGAGCC GCCAGTACCT GCTGCACAGC GAGAAAAATA GGGCCCCTGG AGTTTCCGAG 
GGGCCCGACC TTCCCCCGCC CCTACCGACC AGCCTGCCCT CCCCTGAGCC GCAGCCCTAC 
TCCCTGTTCC GTGCCTACAC CCCCTACACA CGCCATGGGC CCCAGAGTCC CCCCAGAGAC 
CCAACCCTCA CTGTAGCCCC AGAAGAGGCC CTGTCCCCTG GGCAGTCTCA GACAAACGGC 
CCCTCCGGTA CCTCCACCAT GGGTCCCCAG CAGCCTCAGA AGAGTAACTC AGACGAGGAT 
GTGAAGAGAG AGGAGCTGGC CAGAGACATC ATGGACAAGG ATAAGTCCCT GGTAGACATC 
TTGGACCAGA GTAAGATGAA GACCACCATG GATCTGATGG AGGGGATCTT CCCCCAGGGG 
GAGCAGATCT TGGATGGGGG GCACCAGAGG AGGAAAGTCT CCCCCAAACA GGGATTGCCG 
CCCAGGGGAA TGGATGACCG GAGAGAGGAG GAGGGCATGT CTGCTGCCAC AGGGTCCCTG 
GTGACCAGCT CCTCTTACTA CAGCACATCT GCCCCCAAGG CTGAGCTGCT CAACAAGATG 
AAGGACATGC AGGAGGACGA GCTGGAGGAG CTGGAGCAAG ACTCGGAGGA CGAACTGGAC 
ATCAACCTGG CCAGCAAGAA GCAAGAGCTG ATTGACAGCC TGGGTAAGAA GCTGCAGGTG 
CTACGCGAGG CCAGGGAGAG CCTTCAAGAG GATGTCCATG ACAACAACTC TCTGGGGGAC 
GAGGTGGAAG CCGTGGTGCA GAAGGTCTGC AAGCCCAACG AGCTGGACAA GTTCAGGATG 
TTCGTGGGAG ATCTGGACAA GGTGGTCAGT TTGCTGCTGT CCCTGTCTGG CCGACTGGCC 
AGAGTGGAGA ACGCTCTCAA CAGTCTGGAG GAAGACACTA CACCAGAAGA GAAGCGTACC 
CTGTCAGAGA AGAGGAAGTT GTTGATCAGA CAACACGAAG ACGCCAAAGA GCTCAAGGAG 
AACCTTGACC GGCGGGAGCG CCTGGTTTAC AGCATCATGG CTGCTCACCT CAGCCACGAG 
AGCCTGGCAG ACTACCAGCA CTTTGTCAAG ATGAAGTCAG CCCTCATCAT CGAGCAGCGC 
AAGCTGGAAG ACAAGATAAA ACTGGGAGAG GAGCAGCTGA AATGTCTGCT GGATAGTTTA 
TTGTTGGAGC AAAGGCTGCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021427066.1
CDS	2350..6996
Translation	MERVDRPSHPFQPGCLSPSRYHRSAEPLSGGGGSGGKRESAFSCLSSTSPPPEPALANTDTAATESSMFHKGVQTQTSEDGRPGEQRHSRYLQRPQGDGGSESPRTVPEEQPGSRYSSSGSGRSHIGPVWHVPERRRVAAPPSPPLRSDSFAVTKVYPAYTEGPGAPPHGQMKAQDRLAEAPENSSYRGRGNHISHDENGVDPRCSYNPPPCKKDFFHPNIAAGAPDYNHNQLSNPNKLSSVSSQDVRQSQSPFTCLPNHQRQYSDESTFYLQTRSAPHPPKPQSVGSYYHSLQELPTNCTNSRNHVRSSTTSLSTSTIDQNYDCGGHIRYYCITTKQPAQPETHVRQSKSEVWRSDMELAKGSNDRGSTSSSHKTNKVKYPPNPQPAYTNSKERNGNFQPANVLLYGHTTSNSLSSSAKPTTEENERKSEGQRPTEAQLRSSYSPSPPKDHKVGPLREDPWVSQENHKISSQKTPMLHSLAQESRSLAQEIRSPMLHSLAQESKSLVENNHPATAQPPPSNGGGEANHALQEALTDNTATGKLARRSDRYATTLRNEIQLKRAQLQKSRSAATLTCPSETEEPEEEETDPGGWKSTSSTSSDGSFSTSYKDHLKEAQARVLQATSFRRRDLEPPGSGSEAPLTKPNSHIVSRIGSRKRFPLNKRVHSFSEPDKINKLGVEGEGEHPVGTARSFIDRRKFFEMAAKPSFCRPISTIHKSGQQSSSTTSGTLERGEGKARGRAHSGETEEGWRPGLANETSSDHHPDPLSPAGRQALLEQQRLGTFAEYQVTWNMQRKASDTKPQGRYHSADNILDQGTEETPVCVHERSRSSPSQDFHTQKIPMPWRETVENPYLDHRRDQQGGSYTARGQSESRGQPAQLHHFPQPPQPSIPRLTDTEPHSSRDVATPTPLPLPSEHRYKRDSANPAHNLPALPPYPSNHTHSSVSEQPLPPPTPAPKPQNTGLIIMPHWPLLGLETSTTSSSYGLSPQEFLPGPCTHQAEPSSSSSCSSHGTELDPAPNQACSLGSTQLPSPSPRRTTGLGTEEGAADSTLEPPPSPSSHSPFFSYQPASLTVPSSGGTSSPSPHFAPQRLTDQPPVSVSMQDEAQSRPENRTNAVMEMSSVGKKVPVKIVHAESTTERESRQYLLHSEKNRAPGVSEGPDLPPPLPTSLPSPEPQPYSLFRAYTPYTRHGPQSPPRDPTLTVAPEEALSPGQSQTNGPSGTSTMGPQQPQKSNSDEDVKREELARDIMDKDKSLVDILDQSKMKTTMDLMEGIFPQGEQILDGGHQRRKVSPKQGLPPRGMDDRREEEGMSAATGSLVTSSSYYSTSAPKAELLNKMKDMQEDELEELEQDSEDELDINLASKKQELIDSLGKKLQVLREARESLQEDVHDNNSLGDEVEAVVQKVCKPNELDKFRMFVGDLDKVVSLLLSLSGRLARVENALNSLEEDTTPEEKRTLSEKRKLLIRQHEDAKELKENLDRRERLVYSIMAAHLSHESLADYQHFVKMKSALIIEQRKLEDKIKLGEEQLKCLLDSLLLEQRLLF

Target ORF information:

RefSeq Version	XM_021571391.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021571391.1

ORF Insert Sequence:

ATGGAGAGAG TAGATCGCCC CTCACACCCC TTCCAACCAG GATGCCTCTC CCCCAGCAGG 
TACCACCGCA GCGCTGAGCC TCTCTCTGGG GGTGGAGGGT CTGGTGGGAA GAGAGAATCG 
GCCTTCAGTT GCCTCTCCTC CACCAGCCCT CCCCCGGAGC CTGCTCTGGC CAACACTGAC 
ACTGCTGCAA CTGAGAGCAG CATGTTCCAT AAGGGGGTCC AGACTCAGAC CAGTGAGGAT 
GGAAGGCCGG GTGAGCAGCG CCACAGCCGG TACCTCCAGC GGCCCCAAGG GGACGGAGGC 
TCGGAGAGCC CCAGGACAGT CCCAGAGGAG CAGCCTGGCT CCCGCTACTC CAGTTCAGGC 
TCTGGCAGAT CGCACATAGG CCCTGTGTGG CATGTCCCAG AGAGGAGGAG GGTAGCAGCG 
CCCCCCTCTC CTCCCCTGCG CAGTGACAGC TTTGCTGTCA CCAAGGTGTA CCCTGCCTAC 
ACAGAGGGGC CTGGAGCTCC ACCACACGGG CAGATGAAGG CCCAGGACAG GTTGGCAGAG 
GCCCCAGAGA ACAGCAGCTA CAGAGGCCGG GGCAACCATA TCTCTCACGA TGAGAACGGT 
GTAGACCCTA GGTGCAGTTA CAACCCTCCA CCTTGCAAGA AAGACTTCTT CCACCCAAAC 
ATAGCAGCAG GAGCACCTGA CTACAACCAC AACCAGCTCA GCAACCCAAA CAAACTGTCC 
TCTGTGTCTA GCCAGGACGT GAGACAGAGT CAGTCTCCCT TCACTTGCCT GCCCAACCAC 
CAGCGGCAGT ATAGCGACGA AAGCACTTTC TACCTGCAGA CCAGATCCGC CCCACATCCA 
CCGAAGCCGC AGAGCGTCGG TAGCTACTAC CACAGCCTCC AGGAGCTCCC TACCAACTGC 
ACTAACAGCA GGAACCACGT GAGGTCCTCC ACCACGTCCC TGTCCACCTC GACCATCGAC 
CAGAACTATG ACTGCGGAGG ACACATCCGG TACTACTGCA TCACAACCAA GCAGCCAGCC 
CAGCCAGAGA CTCATGTTAG ACAGAGCAAA TCAGAGGTCT GGAGATCTGA CATGGAGCTA 
GCTAAGGGCT CCAACGACAG GGGCTCTACA AGTTCCTCCC ACAAGACCAA CAAGGTCAAG 
TATCCTCCTA ATCCTCAGCC TGCTTACACC AACAGCAAGG AGCGGAACGG AAATTTCCAA 
CCGGCCAATG TTCTGCTTTA CGGCCACACC ACCTCTAATT CCCTATCCTC ATCTGCTAAA 
CCTACCACTG AGGAGAATGA GAGGAAGAGT GAGGGCCAGA GACCTACAGA GGCCCAGCTT 
AGAAGTTCTT ACTCTCCCAG TCCGCCCAAG GACCACAAGG TGGGACCACT GAGAGAAGAC 
CCTTGGGTCT CTCAGGAGAA CCATAAGATC TCTTCTCAAA AGACACCCAT GCTCCACAGT 
CTGGCTCAGG AGAGTAGAAG CCTAGCCCAG GAGATTAGGA GCCCAATGCT TCATTCTCTA 
GCCCAGGAGA GTAAGAGCCT GGTGGAGAAC AATCACCCTG CTACGGCACA ACCACCACCA 
TCCAACGGCG GGGGAGAAGC CAACCACGCC CTACAGGAGG CTTTAACGGA CAACACGGCA 
ACGGGCAAAC TGGCGAGACG CAGCGACCGA TACGCCACCA CCCTCCGCAA CGAGATCCAG 
CTAAAGAGGG CCCAGCTGCA GAAAAGCCGG AGTGCTGCCA CCCTGACCTG CCCCAGCGAG 
ACGGAGGAGC CAGAGGAAGA GGAGACAGAC CCGGGGGGGT GGAAGTCCAC CTCCTCCACC 
TCCTCTGATG GCTCCTTTTC CACGTCCTAC AAGGACCACC TCAAAGAGGC GCAGGCGAGG 
GTCCTCCAGG CCACGTCCTT TAGGAGGAGA GACCTAGAAC CTCCCGGGTC AGGGTCGGAG 
GCTCCGTTAA CCAAACCCAA CTCACACATA GTCTCCCGCA TTGGCAGCCG CAAGCGTTTC 
CCTCTGAACA AGAGGGTCCA CTCGTTCTCC GAGCCAGACA AGATCAACAA GCTCGGAGTG 
GAGGGTGAGG GAGAACATCC TGTTGGAACA GCACGGTCAT TTATAGACCG GCGGAAGTTC 
TTTGAAATGG CTGCTAAACC TTCCTTCTGC AGACCCATCT CAACCATCCA CAAGTCAGGC 
CAGCAGAGCT CCAGCACCAC CTCTGGCACT TTGGAGCGCG GAGAGGGCAA GGCCAGAGGG 
AGAGCCCACT CAGGCGAAAC TGAGGAGGGC TGGAGGCCAG GCCTTGCCAA TGAGACTTCC 
AGTGACCACC ACCCAGACCC CCTCAGCCCC GCAGGCAGAC AGGCCCTACT GGAGCAGCAG 
AGGCTGGGGA CCTTCGCTGA GTACCAGGTC ACCTGGAACA TGCAGAGGAA GGCTTCAGAC 
ACAAAGCCCC AGGGGAGGTA CCACTCTGCT GACAACATCC TGGACCAGGG TACAGAGGAG 
ACGCCTGTCT GTGTCCATGA GAGGTCCAGA TCGTCTCCCT CACAAGACTT CCACACACAG 
AAGATCCCTA TGCCATGGAG AGAGACTGTT GAAAACCCGT ATCTGGATCA CAGACGGGAC 
CAGCAAGGAG GCAGTTACAC CGCCAGAGGG CAGAGCGAGA GCAGAGGGCA GCCAGCCCAG 
CTCCACCACT TCCCACAGCC TCCACAACCG TCTATTCCAA GACTGACCGA CACAGAGCCA 
CACAGCAGCA GAGACGTGGC CACCCCCACC CCCCTCCCTC TCCCCTCCGA ACACAGATAC 
AAACGTGACT CTGCGAACCC CGCCCACAAC CTCCCCGCTC TCCCCCCGTA CCCCTCCAAT 
CACACCCACA GCTCTGTGTC TGAGCAACCA CTACCACCTC CAACGCCCGC TCCCAAGCCC 
CAGAATACAG GCCTCATCAT CATGCCACAT TGGCCTCTCC TAGGCCTGGA AACCTCAACC 
ACATCCTCCT CCTACGGACT GTCTCCCCAG GAATTCCTGC CTGGCCCTTG CACCCATCAG 
GCTGAACCAT CATCCAGCAG CAGCTGCTCC TCCCATGGCA CAGAGCTGGA TCCTGCCCCA 
AACCAAGCCT GCTCCTTGGG CTCCACCCAG CTCCCCTCTC CCTCCCCTCG GAGAACCACT 
GGACTGGGCA CGGAGGAGGG AGCAGCTGAT TCAACACTAG AGCCGCCACC CTCCCCCTCT 
TCCCACTCTC CTTTTTTCTC CTACCAGCCT GCCAGTCTGA CGGTTCCCTC CTCAGGTGGG 
ACTAGTTCTC CCTCTCCTCA TTTTGCTCCA CAGAGGTTGA CGGACCAACC CCCTGTCTCT 
GTGTCTATGC AGGATGAAGC CCAGAGCAGG CCAGAGAACA GGACCAACGC TGTGATGGAG 
ATGAGCAGTG TAGGGAAGAA GGTCCCTGTT AAAATCGTCC ATGCTGAGAG CACCACAGAG 
AGGGAGAGCC GCCAGTACCT GCTGCACAGC GAGAAAAATA GGGCCCCTGG AGTTTCCGAG 
GGGCCCGACC TTCCCCCGCC CCTACCGACC AGCCTGCCCT CCCCTGAGCC GCAGCCCTAC 
TCCCTGTTCC GTGCCTACAC CCCCTACACA CGCCATGGGC CCCAGAGTCC CCCCAGAGAC 
CCAACCCTCA CTGTAGCCCC AGAAGAGGCC CTGTCCCCTG GGCAGTCTCA GACAAACGGC 
CCCTCCGGTA CCTCCACCAT GGGTCCCCAG CAGCCTCAGA AGAGTAACTC AGACGAGGAT 
GTGAAGAGAG AGGAGCTGGC CAGAGACATC ATGGACAAGG ATAAGTCCCT GGTAGACATC 
TTGGACCAGA GTAAGATGAA GACCACCATG GATCTGATGG AGGGGATCTT CCCCCAGGGG 
GAGCAGATCT TGGATGGGGG GCACCAGAGG AGGAAAGTCT CCCCCAAACA GGGATTGCCG 
CCCAGGGGAA TGGATGACCG GAGAGAGGAG GAGGGCATGT CTGCTGCCAC AGGGTCCCTG 
GTGACCAGCT CCTCTTACTA CAGCACATCT GCCCCCAAGG CTGAGCTGCT CAACAAGATG 
AAGGACATGC AGGAGGACGA GCTGGAGGAG CTGGAGCAAG ACTCGGAGGA CGAACTGGAC 
ATCAACCTGG CCAGCAAGAA GCAAGAGCTG ATTGACAGCC TGGGTAAGAA GCTGCAGGTG 
CTACGCGAGG CCAGGGAGAG CCTTCAAGAG GATGTCCATG ACAACAACTC TCTGGGGGAC 
GAGGTGGAAG CCGTGGTGCA GAAGGTCTGC AAGCCCAACG AGCTGGACAA GTTCAGGATG 
TTCGTGGGAG ATCTGGACAA GGTGGTCAGT TTGCTGCTGT CCCTGTCTGG CCGACTGGCC 
AGAGTGGAGA ACGCTCTCAA CAGTCTGGAG GAAGACACTA CACCAGAAGA GAAGCGTACC 
CTGTCAGAGA AGAGGAAGTT GTTGATCAGA CAACACGAAG ACGCCAAAGA GCTCAAGGAG 
AACCTTGACC GGCGGGAGCG CCTGGTTTAC AGCATCATGG CTGCTCACCT CAGCCACGAG 
AGCCTGGCAG ACTACCAGCA CTTTGTCAAG ATGAAGTCAG CCCTCATCAT CGAGCAGCGC 
AAGCTGGAAG ACAAGATAAA ACTGGGAGAG GAGCAGCTGA AATGTCTGCT GGATAGTTTA 
TTGTTGGAGC AAAGGCTGCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110495881 ( XM_021571392.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021427067.1 Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X8, mRNA.

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CloneID	OOf19776
Clone ID Related Accession (Same CDS sequence)	XM_021571391.1 , XM_021571392.1
Accession Version	XM_021571392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	protein Shroom2-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG TAGATCGCCC CTCACACCCC TTCCAACCAG GATGCCTCTC CCCCAGCAGG 
TACCACCGCA GCGCTGAGCC TCTCTCTGGG GGTGGAGGGT CTGGTGGGAA GAGAGAATCG 
GCCTTCAGTT GCCTCTCCTC CACCAGCCCT CCCCCGGAGC CTGCTCTGGC CAACACTGAC 
ACTGCTGCAA CTGAGAGCAG CATGTTCCAT AAGGGGGTCC AGACTCAGAC CAGTGAGGAT 
GGAAGGCCGG GTGAGCAGCG CCACAGCCGG TACCTCCAGC GGCCCCAAGG GGACGGAGGC 
TCGGAGAGCC CCAGGACAGT CCCAGAGGAG CAGCCTGGCT CCCGCTACTC CAGTTCAGGC 
TCTGGCAGAT CGCACATAGG CCCTGTGTGG CATGTCCCAG AGAGGAGGAG GGTAGCAGCG 
CCCCCCTCTC CTCCCCTGCG CAGTGACAGC TTTGCTGTCA CCAAGGTGTA CCCTGCCTAC 
ACAGAGGGGC CTGGAGCTCC ACCACACGGG CAGATGAAGG CCCAGGACAG GTTGGCAGAG 
GCCCCAGAGA ACAGCAGCTA CAGAGGCCGG GGCAACCATA TCTCTCACGA TGAGAACGGT 
GTAGACCCTA GGTGCAGTTA CAACCCTCCA CCTTGCAAGA AAGACTTCTT CCACCCAAAC 
ATAGCAGCAG GAGCACCTGA CTACAACCAC AACCAGCTCA GCAACCCAAA CAAACTGTCC 
TCTGTGTCTA GCCAGGACGT GAGACAGAGT CAGTCTCCCT TCACTTGCCT GCCCAACCAC 
CAGCGGCAGT ATAGCGACGA AAGCACTTTC TACCTGCAGA CCAGATCCGC CCCACATCCA 
CCGAAGCCGC AGAGCGTCGG TAGCTACTAC CACAGCCTCC AGGAGCTCCC TACCAACTGC 
ACTAACAGCA GGAACCACGT GAGGTCCTCC ACCACGTCCC TGTCCACCTC GACCATCGAC 
CAGAACTATG ACTGCGGAGG ACACATCCGG TACTACTGCA TCACAACCAA GCAGCCAGCC 
CAGCCAGAGA CTCATGTTAG ACAGAGCAAA TCAGAGGTCT GGAGATCTGA CATGGAGCTA 
GCTAAGGGCT CCAACGACAG GGGCTCTACA AGTTCCTCCC ACAAGACCAA CAAGGTCAAG 
TATCCTCCTA ATCCTCAGCC TGCTTACACC AACAGCAAGG AGCGGAACGG AAATTTCCAA 
CCGGCCAATG TTCTGCTTTA CGGCCACACC ACCTCTAATT CCCTATCCTC ATCTGCTAAA 
CCTACCACTG AGGAGAATGA GAGGAAGAGT GAGGGCCAGA GACCTACAGA GGCCCAGCTT 
AGAAGTTCTT ACTCTCCCAG TCCGCCCAAG GACCACAAGG TGGGACCACT GAGAGAAGAC 
CCTTGGGTCT CTCAGGAGAA CCATAAGATC TCTTCTCAAA AGACACCCAT GCTCCACAGT 
CTGGCTCAGG AGAGTAGAAG CCTAGCCCAG GAGATTAGGA GCCCAATGCT TCATTCTCTA 
GCCCAGGAGA GTAAGAGCCT GGTGGAGAAC AATCACCCTG CTACGGCACA ACCACCACCA 
TCCAACGGCG GGGGAGAAGC CAACCACGCC CTACAGGAGG CTTTAACGGA CAACACGGCA 
ACGGGCAAAC TGGCGAGACG CAGCGACCGA TACGCCACCA CCCTCCGCAA CGAGATCCAG 
CTAAAGAGGG CCCAGCTGCA GAAAAGCCGG AGTGCTGCCA CCCTGACCTG CCCCAGCGAG 
ACGGAGGAGC CAGAGGAAGA GGAGACAGAC CCGGGGGGGT GGAAGTCCAC CTCCTCCACC 
TCCTCTGATG GCTCCTTTTC CACGTCCTAC AAGGACCACC TCAAAGAGGC GCAGGCGAGG 
GTCCTCCAGG CCACGTCCTT TAGGAGGAGA GACCTAGAAC CTCCCGGGTC AGGGTCGGAG 
GCTCCGTTAA CCAAACCCAA CTCACACATA GTCTCCCGCA TTGGCAGCCG CAAGCGTTTC 
CCTCTGAACA AGAGGGTCCA CTCGTTCTCC GAGCCAGACA AGATCAACAA GCTCGGAGTG 
GAGGGTGAGG GAGAACATCC TGTTGGAACA GCACGGTCAT TTATAGACCG GCGGAAGTTC 
TTTGAAATGG CTGCTAAACC TTCCTTCTGC AGACCCATCT CAACCATCCA CAAGTCAGGC 
CAGCAGAGCT CCAGCACCAC CTCTGGCACT TTGGAGCGCG GAGAGGGCAA GGCCAGAGGG 
AGAGCCCACT CAGGCGAAAC TGAGGAGGGC TGGAGGCCAG GCCTTGCCAA TGAGACTTCC 
AGTGACCACC ACCCAGACCC CCTCAGCCCC GCAGGCAGAC AGGCCCTACT GGAGCAGCAG 
AGGCTGGGGA CCTTCGCTGA GTACCAGGTC ACCTGGAACA TGCAGAGGAA GGCTTCAGAC 
ACAAAGCCCC AGGGGAGGTA CCACTCTGCT GACAACATCC TGGACCAGGG TACAGAGGAG 
ACGCCTGTCT GTGTCCATGA GAGGTCCAGA TCGTCTCCCT CACAAGACTT CCACACACAG 
AAGATCCCTA TGCCATGGAG AGAGACTGTT GAAAACCCGT ATCTGGATCA CAGACGGGAC 
CAGCAAGGAG GCAGTTACAC CGCCAGAGGG CAGAGCGAGA GCAGAGGGCA GCCAGCCCAG 
CTCCACCACT TCCCACAGCC TCCACAACCG TCTATTCCAA GACTGACCGA CACAGAGCCA 
CACAGCAGCA GAGACGTGGC CACCCCCACC CCCCTCCCTC TCCCCTCCGA ACACAGATAC 
AAACGTGACT CTGCGAACCC CGCCCACAAC CTCCCCGCTC TCCCCCCGTA CCCCTCCAAT 
CACACCCACA GCTCTGTGTC TGAGCAACCA CTACCACCTC CAACGCCCGC TCCCAAGCCC 
CAGAATACAG GCCTCATCAT CATGCCACAT TGGCCTCTCC TAGGCCTGGA AACCTCAACC 
ACATCCTCCT CCTACGGACT GTCTCCCCAG GAATTCCTGC CTGGCCCTTG CACCCATCAG 
GCTGAACCAT CATCCAGCAG CAGCTGCTCC TCCCATGGCA CAGAGCTGGA TCCTGCCCCA 
AACCAAGCCT GCTCCTTGGG CTCCACCCAG CTCCCCTCTC CCTCCCCTCG GAGAACCACT 
GGACTGGGCA CGGAGGAGGG AGCAGCTGAT TCAACACTAG AGCCGCCACC CTCCCCCTCT 
TCCCACTCTC CTTTTTTCTC CTACCAGCCT GCCAGTCTGA CGGTTCCCTC CTCAGGTGGG 
ACTAGTTCTC CCTCTCCTCA TTTTGCTCCA CAGAGGTTGA CGGACCAACC CCCTGTCTCT 
GTGTCTATGC AGGATGAAGC CCAGAGCAGG CCAGAGAACA GGACCAACGC TGTGATGGAG 
ATGAGCAGTG TAGGGAAGAA GGTCCCTGTT AAAATCGTCC ATGCTGAGAG CACCACAGAG 
AGGGAGAGCC GCCAGTACCT GCTGCACAGC GAGAAAAATA GGGCCCCTGG AGTTTCCGAG 
GGGCCCGACC TTCCCCCGCC CCTACCGACC AGCCTGCCCT CCCCTGAGCC GCAGCCCTAC 
TCCCTGTTCC GTGCCTACAC CCCCTACACA CGCCATGGGC CCCAGAGTCC CCCCAGAGAC 
CCAACCCTCA CTGTAGCCCC AGAAGAGGCC CTGTCCCCTG GGCAGTCTCA GACAAACGGC 
CCCTCCGGTA CCTCCACCAT GGGTCCCCAG CAGCCTCAGA AGAGTAACTC AGACGAGGAT 
GTGAAGAGAG AGGAGCTGGC CAGAGACATC ATGGACAAGG ATAAGTCCCT GGTAGACATC 
TTGGACCAGA GTAAGATGAA GACCACCATG GATCTGATGG AGGGGATCTT CCCCCAGGGG 
GAGCAGATCT TGGATGGGGG GCACCAGAGG AGGAAAGTCT CCCCCAAACA GGGATTGCCG 
CCCAGGGGAA TGGATGACCG GAGAGAGGAG GAGGGCATGT CTGCTGCCAC AGGGTCCCTG 
GTGACCAGCT CCTCTTACTA CAGCACATCT GCCCCCAAGG CTGAGCTGCT CAACAAGATG 
AAGGACATGC AGGAGGACGA GCTGGAGGAG CTGGAGCAAG ACTCGGAGGA CGAACTGGAC 
ATCAACCTGG CCAGCAAGAA GCAAGAGCTG ATTGACAGCC TGGGTAAGAA GCTGCAGGTG 
CTACGCGAGG CCAGGGAGAG CCTTCAAGAG GATGTCCATG ACAACAACTC TCTGGGGGAC 
GAGGTGGAAG CCGTGGTGCA GAAGGTCTGC AAGCCCAACG AGCTGGACAA GTTCAGGATG 
TTCGTGGGAG ATCTGGACAA GGTGGTCAGT TTGCTGCTGT CCCTGTCTGG CCGACTGGCC 
AGAGTGGAGA ACGCTCTCAA CAGTCTGGAG GAAGACACTA CACCAGAAGA GAAGCGTACC 
CTGTCAGAGA AGAGGAAGTT GTTGATCAGA CAACACGAAG ACGCCAAAGA GCTCAAGGAG 
AACCTTGACC GGCGGGAGCG CCTGGTTTAC AGCATCATGG CTGCTCACCT CAGCCACGAG 
AGCCTGGCAG ACTACCAGCA CTTTGTCAAG ATGAAGTCAG CCCTCATCAT CGAGCAGCGC 
AAGCTGGAAG ACAAGATAAA ACTGGGAGAG GAGCAGCTGA AATGTCTGCT GGATAGTTTA 
TTGTTGGAGC AAAGGCTGCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021427067.1
CDS	407..5053
Translation	MERVDRPSHPFQPGCLSPSRYHRSAEPLSGGGGSGGKRESAFSCLSSTSPPPEPALANTDTAATESSMFHKGVQTQTSEDGRPGEQRHSRYLQRPQGDGGSESPRTVPEEQPGSRYSSSGSGRSHIGPVWHVPERRRVAAPPSPPLRSDSFAVTKVYPAYTEGPGAPPHGQMKAQDRLAEAPENSSYRGRGNHISHDENGVDPRCSYNPPPCKKDFFHPNIAAGAPDYNHNQLSNPNKLSSVSSQDVRQSQSPFTCLPNHQRQYSDESTFYLQTRSAPHPPKPQSVGSYYHSLQELPTNCTNSRNHVRSSTTSLSTSTIDQNYDCGGHIRYYCITTKQPAQPETHVRQSKSEVWRSDMELAKGSNDRGSTSSSHKTNKVKYPPNPQPAYTNSKERNGNFQPANVLLYGHTTSNSLSSSAKPTTEENERKSEGQRPTEAQLRSSYSPSPPKDHKVGPLREDPWVSQENHKISSQKTPMLHSLAQESRSLAQEIRSPMLHSLAQESKSLVENNHPATAQPPPSNGGGEANHALQEALTDNTATGKLARRSDRYATTLRNEIQLKRAQLQKSRSAATLTCPSETEEPEEEETDPGGWKSTSSTSSDGSFSTSYKDHLKEAQARVLQATSFRRRDLEPPGSGSEAPLTKPNSHIVSRIGSRKRFPLNKRVHSFSEPDKINKLGVEGEGEHPVGTARSFIDRRKFFEMAAKPSFCRPISTIHKSGQQSSSTTSGTLERGEGKARGRAHSGETEEGWRPGLANETSSDHHPDPLSPAGRQALLEQQRLGTFAEYQVTWNMQRKASDTKPQGRYHSADNILDQGTEETPVCVHERSRSSPSQDFHTQKIPMPWRETVENPYLDHRRDQQGGSYTARGQSESRGQPAQLHHFPQPPQPSIPRLTDTEPHSSRDVATPTPLPLPSEHRYKRDSANPAHNLPALPPYPSNHTHSSVSEQPLPPPTPAPKPQNTGLIIMPHWPLLGLETSTTSSSYGLSPQEFLPGPCTHQAEPSSSSSCSSHGTELDPAPNQACSLGSTQLPSPSPRRTTGLGTEEGAADSTLEPPPSPSSHSPFFSYQPASLTVPSSGGTSSPSPHFAPQRLTDQPPVSVSMQDEAQSRPENRTNAVMEMSSVGKKVPVKIVHAESTTERESRQYLLHSEKNRAPGVSEGPDLPPPLPTSLPSPEPQPYSLFRAYTPYTRHGPQSPPRDPTLTVAPEEALSPGQSQTNGPSGTSTMGPQQPQKSNSDEDVKREELARDIMDKDKSLVDILDQSKMKTTMDLMEGIFPQGEQILDGGHQRRKVSPKQGLPPRGMDDRREEEGMSAATGSLVTSSSYYSTSAPKAELLNKMKDMQEDELEELEQDSEDELDINLASKKQELIDSLGKKLQVLREARESLQEDVHDNNSLGDEVEAVVQKVCKPNELDKFRMFVGDLDKVVSLLLSLSGRLARVENALNSLEEDTTPEEKRTLSEKRKLLIRQHEDAKELKENLDRRERLVYSIMAAHLSHESLADYQHFVKMKSALIIEQRKLEDKIKLGEEQLKCLLDSLLLEQRLLF

Target ORF information:

RefSeq Version	XM_021571392.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss protein Shroom2-like (LOC110495881), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021571392.1

ORF Insert Sequence:

ATGGAGAGAG TAGATCGCCC CTCACACCCC TTCCAACCAG GATGCCTCTC CCCCAGCAGG 
TACCACCGCA GCGCTGAGCC TCTCTCTGGG GGTGGAGGGT CTGGTGGGAA GAGAGAATCG 
GCCTTCAGTT GCCTCTCCTC CACCAGCCCT CCCCCGGAGC CTGCTCTGGC CAACACTGAC 
ACTGCTGCAA CTGAGAGCAG CATGTTCCAT AAGGGGGTCC AGACTCAGAC CAGTGAGGAT 
GGAAGGCCGG GTGAGCAGCG CCACAGCCGG TACCTCCAGC GGCCCCAAGG GGACGGAGGC 
TCGGAGAGCC CCAGGACAGT CCCAGAGGAG CAGCCTGGCT CCCGCTACTC CAGTTCAGGC 
TCTGGCAGAT CGCACATAGG CCCTGTGTGG CATGTCCCAG AGAGGAGGAG GGTAGCAGCG 
CCCCCCTCTC CTCCCCTGCG CAGTGACAGC TTTGCTGTCA CCAAGGTGTA CCCTGCCTAC 
ACAGAGGGGC CTGGAGCTCC ACCACACGGG CAGATGAAGG CCCAGGACAG GTTGGCAGAG 
GCCCCAGAGA ACAGCAGCTA CAGAGGCCGG GGCAACCATA TCTCTCACGA TGAGAACGGT 
GTAGACCCTA GGTGCAGTTA CAACCCTCCA CCTTGCAAGA AAGACTTCTT CCACCCAAAC 
ATAGCAGCAG GAGCACCTGA CTACAACCAC AACCAGCTCA GCAACCCAAA CAAACTGTCC 
TCTGTGTCTA GCCAGGACGT GAGACAGAGT CAGTCTCCCT TCACTTGCCT GCCCAACCAC 
CAGCGGCAGT ATAGCGACGA AAGCACTTTC TACCTGCAGA CCAGATCCGC CCCACATCCA 
CCGAAGCCGC AGAGCGTCGG TAGCTACTAC CACAGCCTCC AGGAGCTCCC TACCAACTGC 
ACTAACAGCA GGAACCACGT GAGGTCCTCC ACCACGTCCC TGTCCACCTC GACCATCGAC 
CAGAACTATG ACTGCGGAGG ACACATCCGG TACTACTGCA TCACAACCAA GCAGCCAGCC 
CAGCCAGAGA CTCATGTTAG ACAGAGCAAA TCAGAGGTCT GGAGATCTGA CATGGAGCTA 
GCTAAGGGCT CCAACGACAG GGGCTCTACA AGTTCCTCCC ACAAGACCAA CAAGGTCAAG 
TATCCTCCTA ATCCTCAGCC TGCTTACACC AACAGCAAGG AGCGGAACGG AAATTTCCAA 
CCGGCCAATG TTCTGCTTTA CGGCCACACC ACCTCTAATT CCCTATCCTC ATCTGCTAAA 
CCTACCACTG AGGAGAATGA GAGGAAGAGT GAGGGCCAGA GACCTACAGA GGCCCAGCTT 
AGAAGTTCTT ACTCTCCCAG TCCGCCCAAG GACCACAAGG TGGGACCACT GAGAGAAGAC 
CCTTGGGTCT CTCAGGAGAA CCATAAGATC TCTTCTCAAA AGACACCCAT GCTCCACAGT 
CTGGCTCAGG AGAGTAGAAG CCTAGCCCAG GAGATTAGGA GCCCAATGCT TCATTCTCTA 
GCCCAGGAGA GTAAGAGCCT GGTGGAGAAC AATCACCCTG CTACGGCACA ACCACCACCA 
TCCAACGGCG GGGGAGAAGC CAACCACGCC CTACAGGAGG CTTTAACGGA CAACACGGCA 
ACGGGCAAAC TGGCGAGACG CAGCGACCGA TACGCCACCA CCCTCCGCAA CGAGATCCAG 
CTAAAGAGGG CCCAGCTGCA GAAAAGCCGG AGTGCTGCCA CCCTGACCTG CCCCAGCGAG 
ACGGAGGAGC CAGAGGAAGA GGAGACAGAC CCGGGGGGGT GGAAGTCCAC CTCCTCCACC 
TCCTCTGATG GCTCCTTTTC CACGTCCTAC AAGGACCACC TCAAAGAGGC GCAGGCGAGG 
GTCCTCCAGG CCACGTCCTT TAGGAGGAGA GACCTAGAAC CTCCCGGGTC AGGGTCGGAG 
GCTCCGTTAA CCAAACCCAA CTCACACATA GTCTCCCGCA TTGGCAGCCG CAAGCGTTTC 
CCTCTGAACA AGAGGGTCCA CTCGTTCTCC GAGCCAGACA AGATCAACAA GCTCGGAGTG 
GAGGGTGAGG GAGAACATCC TGTTGGAACA GCACGGTCAT TTATAGACCG GCGGAAGTTC 
TTTGAAATGG CTGCTAAACC TTCCTTCTGC AGACCCATCT CAACCATCCA CAAGTCAGGC 
CAGCAGAGCT CCAGCACCAC CTCTGGCACT TTGGAGCGCG GAGAGGGCAA GGCCAGAGGG 
AGAGCCCACT CAGGCGAAAC TGAGGAGGGC TGGAGGCCAG GCCTTGCCAA TGAGACTTCC 
AGTGACCACC ACCCAGACCC CCTCAGCCCC GCAGGCAGAC AGGCCCTACT GGAGCAGCAG 
AGGCTGGGGA CCTTCGCTGA GTACCAGGTC ACCTGGAACA TGCAGAGGAA GGCTTCAGAC 
ACAAAGCCCC AGGGGAGGTA CCACTCTGCT GACAACATCC TGGACCAGGG TACAGAGGAG 
ACGCCTGTCT GTGTCCATGA GAGGTCCAGA TCGTCTCCCT CACAAGACTT CCACACACAG 
AAGATCCCTA TGCCATGGAG AGAGACTGTT GAAAACCCGT ATCTGGATCA CAGACGGGAC 
CAGCAAGGAG GCAGTTACAC CGCCAGAGGG CAGAGCGAGA GCAGAGGGCA GCCAGCCCAG 
CTCCACCACT TCCCACAGCC TCCACAACCG TCTATTCCAA GACTGACCGA CACAGAGCCA 
CACAGCAGCA GAGACGTGGC CACCCCCACC CCCCTCCCTC TCCCCTCCGA ACACAGATAC 
AAACGTGACT CTGCGAACCC CGCCCACAAC CTCCCCGCTC TCCCCCCGTA CCCCTCCAAT 
CACACCCACA GCTCTGTGTC TGAGCAACCA CTACCACCTC CAACGCCCGC TCCCAAGCCC 
CAGAATACAG GCCTCATCAT CATGCCACAT TGGCCTCTCC TAGGCCTGGA AACCTCAACC 
ACATCCTCCT CCTACGGACT GTCTCCCCAG GAATTCCTGC CTGGCCCTTG CACCCATCAG 
GCTGAACCAT CATCCAGCAG CAGCTGCTCC TCCCATGGCA CAGAGCTGGA TCCTGCCCCA 
AACCAAGCCT GCTCCTTGGG CTCCACCCAG CTCCCCTCTC CCTCCCCTCG GAGAACCACT 
GGACTGGGCA CGGAGGAGGG AGCAGCTGAT TCAACACTAG AGCCGCCACC CTCCCCCTCT 
TCCCACTCTC CTTTTTTCTC CTACCAGCCT GCCAGTCTGA CGGTTCCCTC CTCAGGTGGG 
ACTAGTTCTC CCTCTCCTCA TTTTGCTCCA CAGAGGTTGA CGGACCAACC CCCTGTCTCT 
GTGTCTATGC AGGATGAAGC CCAGAGCAGG CCAGAGAACA GGACCAACGC TGTGATGGAG 
ATGAGCAGTG TAGGGAAGAA GGTCCCTGTT AAAATCGTCC ATGCTGAGAG CACCACAGAG 
AGGGAGAGCC GCCAGTACCT GCTGCACAGC GAGAAAAATA GGGCCCCTGG AGTTTCCGAG 
GGGCCCGACC TTCCCCCGCC CCTACCGACC AGCCTGCCCT CCCCTGAGCC GCAGCCCTAC 
TCCCTGTTCC GTGCCTACAC CCCCTACACA CGCCATGGGC CCCAGAGTCC CCCCAGAGAC 
CCAACCCTCA CTGTAGCCCC AGAAGAGGCC CTGTCCCCTG GGCAGTCTCA GACAAACGGC 
CCCTCCGGTA CCTCCACCAT GGGTCCCCAG CAGCCTCAGA AGAGTAACTC AGACGAGGAT 
GTGAAGAGAG AGGAGCTGGC CAGAGACATC ATGGACAAGG ATAAGTCCCT GGTAGACATC 
TTGGACCAGA GTAAGATGAA GACCACCATG GATCTGATGG AGGGGATCTT CCCCCAGGGG 
GAGCAGATCT TGGATGGGGG GCACCAGAGG AGGAAAGTCT CCCCCAAACA GGGATTGCCG 
CCCAGGGGAA TGGATGACCG GAGAGAGGAG GAGGGCATGT CTGCTGCCAC AGGGTCCCTG 
GTGACCAGCT CCTCTTACTA CAGCACATCT GCCCCCAAGG CTGAGCTGCT CAACAAGATG 
AAGGACATGC AGGAGGACGA GCTGGAGGAG CTGGAGCAAG ACTCGGAGGA CGAACTGGAC 
ATCAACCTGG CCAGCAAGAA GCAAGAGCTG ATTGACAGCC TGGGTAAGAA GCTGCAGGTG 
CTACGCGAGG CCAGGGAGAG CCTTCAAGAG GATGTCCATG ACAACAACTC TCTGGGGGAC 
GAGGTGGAAG CCGTGGTGCA GAAGGTCTGC AAGCCCAACG AGCTGGACAA GTTCAGGATG 
TTCGTGGGAG ATCTGGACAA GGTGGTCAGT TTGCTGCTGT CCCTGTCTGG CCGACTGGCC 
AGAGTGGAGA ACGCTCTCAA CAGTCTGGAG GAAGACACTA CACCAGAAGA GAAGCGTACC 
CTGTCAGAGA AGAGGAAGTT GTTGATCAGA CAACACGAAG ACGCCAAAGA GCTCAAGGAG 
AACCTTGACC GGCGGGAGCG CCTGGTTTAC AGCATCATGG CTGCTCACCT CAGCCACGAG 
AGCCTGGCAG ACTACCAGCA CTTTGTCAAG ATGAAGTCAG CCCTCATCAT CGAGCAGCGC 
AAGCTGGAAG ACAAGATAAA ACTGGGAGAG GAGCAGCTGA AATGTCTGCT GGATAGTTTA 
TTGTTGGAGC AAAGGCTGCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC110495881 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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