atp13a2 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	atp13a2
Entrez Gene ID	110492246
Full Name	ATPase 13A2
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021566391.1	XP_021422066.1	cation-transporting ATPase 13A2 isoform X2
XM_021566390.1	XP_021422065.1	cation-transporting ATPase 13A2 isoform X1
XM_021566392.1	XP_021422067.1	cation-transporting ATPase 13A2 isoform X3

Pathways from BioSystems
Gene Ontology

The following atp13a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atp13a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOf16009	XM_021566390.1 Latest version!	Oncorhynchus mykiss ATPase 13A2 (atp13a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOf16010	XM_021566391.1 Latest version!	Oncorhynchus mykiss ATPase 13A2 (atp13a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOf16011	XM_021566392.1 Latest version!	Oncorhynchus mykiss ATPase 13A2 (atp13a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atp13a2 ( XM_021566390.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021422065.1 Oncorhynchus mykiss ATPase 13A2 (atp13a2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf16009
Clone ID Related Accession (Same CDS sequence)	XM_021566390.1
Accession Version	XM_021566390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	cation-transporting ATPase 13A2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAG GAAGGATGGA CACGCGTGGT TGTCCGGAGG AGGAGCCACC GGGAGGGTTC 
TGCTCCCCTA GTCCAAGGAG AGAACCCCAC TGCAAACCCG AAACACACAT GGATGTCCAG 
GGGTTCAGAT GGGTGCGCTG GCGTGTCTGG CTGTGTCACA TTGGGTCCGT GTTGTCCCTG 
GGTCTGTTGC TGGTGCTGTT TCGCTGGCGC CCTCGTCTAG GGGTCCTTGC TCGCTGCAAC 
TCCTGTCCCC TGGCCTTCGC CAATATTCTG CTTCTGAGGG ACAGTTTTGG ACAGCAGTAT 
GTCGTGGAGG TCCTCACAGA AGAGCTGGAG GAGGGCAGTT TGGAGTCGGC AGGGAGTGAG 
GCGGAGGACA GTTCAGACTG GAGAGATACT GTCCAGCTAC ACAAAGAGGA GGTACCGCTG 
CTGCATTACT ACCTGTTTGA GGGGCTGCGC TACGTATGGA TGGCCAGGAA GGGAGCCTTC 
TGTCGTGTTA GTGTCCTCAA TGAGGACTGG ACATGTGCCG ACATTTATCG TTTCCGGGAG 
GGCCTGAGTC GCCTTGAGCA GAACACCAGG AGGAAGATCT ACGGGACCAA TCTCATCAAT 
GTGCCTGTGA AGTCCTTCAT GCGACTACTG ATTGAGGAGG TGCTCAACCC ATTCTACGTG 
TTCCAGGTGT TCAGCATTGC TTTGTGGCTG ACAGATAGTT ACTACTACTA TGCTGCTTGC 
ATCGTCATCA TCTCCCTTAT CTCCATATGT GTGTCTCTCT ACGAGATCCG CAAGCAAAGC 
AACACGATCC GTAGCATGGC TCAGCAGATA ACCAATGTTA CCGTGCGCAG AAGCTCCGGG 
GAGGAGGACA GTTTGAGCTC TGTGGAGCTG GTCCCGGGGG ACTGTGTGCT GATCCCTCAG 
GAGGGGCTGC TGCTGCCCTG CGACGCTGCT CTGCTGGCCG GGGAGTGCAT GGTCAACGAG 
AGTATGCTAA CCGGTGAGAG TGTCCCTGTG ATGAAGACCC CGCTGCCGGC TGCTGGGGGG 
CGTTACAGCC CTGAGACTCA GCGCAGACAC ACCCTCTTCT GCGGCACGCA GTGCATCCAG 
GCCAAAGGGG GCCGGCCAGG AAGGGGCGGG GCCATTGCCG TGGTCACAAG CACAGGGTTC 
TTCACAGCCA AGGGAGACCT GGTCAGCTCC ATCCTCTACC CCCAACCTAT CAACTTCCGC 
TTCTACCAAG ACGCTATTAA GTTCTTGCTT TTCCTGGGGG GTGTAGCATT GTGTGGCACA 
GTGTACAGCA TCGTGATCCT AAGCAGAACC AACACCACGT GGTGGGAGCT GGTGATTCGG 
GCTCTGGATA TTGTGACCAT TGTGGTGCCC CCTGCCCTCC CCGCTGCTAT CACCACCGGT 
ACCATTTACG CCCAGAGTCG TCTGAAGAGA GAAGGGGTCT TCTGCATAAG CCCCCCGCGC 
ATCAACATCT CTGGGAAGGT CTCGCTCTTT TGCTTTGACA AGACAGGCAC TCTAACTGAG 
GAGGGACTGG ACGTGTGGGG GGTGATGGAG GGTAGTGGAG CTGGGTTCTC TGAGCTGGTC 
CCTGACCCAC GCCTCCTGCC CCAGGGGCCC ATGCTCTCTG CAATGGCCTG CTGCCACTCT 
GTGGCCTTGC TGGGGGGACA ACCCCTGGGA GACCCTCTAG AGCTCAAGAT GATTGAGTCC 
ACTGGCTGGG AGCTGGAGGA GCCTGCAGGA GATGACAAAA CGTTAGACTC AGATTTCGGA 
GGTCACAGGG TTCTGGCAGT GATGCGACCC CCTGCCTGTC CGCTCCAATC TGAGAGTGTA 
TCCATCCATG AGGCGGTGGC CCTCGTGCAG CGTTTCCCTT TCTCCTCGTC CCTCCAGCGG 
ATGAGTGTGG TGACGGTGAA TCCTGAGGGA CAGTCGTCCC TCGCCTTTAT CAAAGGGGCC 
CCAGAGATGG TGGCCAGCCT CTGCCTGAGA GAAAGCGTGC CTGCACAATT CTCAAACACC 
CTGCGTACTT TCGCAAGTGA CGGCTTCAGG GTGCTAGCTC TCGGCTACAA AACACTGTAT 
GGGCAGACTG ACTTGAGCAC CATTGAACGG GGAGAGGTAG AGCGAGACAT GCAGTTCCTG 
GGTCTGCTGG TGATGAAGAA TCTGGTCAAG CCGGAGAGTG CCGAGGTCAT AACTACCCTC 
AGATTGGCAC ATCTTCGTAC CGTCATGGTC ACTGGTGACA ACGTTCTGAC TGCAGTCAAT 
GTGGCTCGAA CCTGTGGGAT GTTGGGCTCA AACGAGAGGG TGATTTTTGT TCACGCCACC 
CCCCAGACGG CCCAGTCTCT GCCCTCACTG CGCTTCCACC TGGGGGAAGG AGGGACGGAG 
GACACAGCTA CACAGAGCTC CATAGACATG TACACTCAGG GTCTGTACCA GGGTGTTATC 
AACTATCACC TGGCCATCAA TGGAAAGTCC TTCTCCGCGC TGTGTGACCA CTTCCCTGAG 
TATCTGCCAA AGGTGTTGAT GCGGGGAACG GTCTACGCAC GGATGGCCCC TGAACAGAAG 
ACTCAGCTGG TCACAGAGCT GCAGAAACTG AACTACCGTG TGGGCATGTG CGGAGACGGG 
GCCAACGACT GCGGAGCCCT AAGGGCCGCA GACGTAGGCG TGTCTCTGTC AGAGGCAGAG 
GCATCCGTGG CCTCTCCCTT CACCTCCAAG TCAGACAACA TCAGCTGTGT GCCTCTACTC 
ATCCGGGAGG GCCGGTGTTC TCTGGTGACA TCATTCAGTC TCTTCAAGTA CATGGCCTTG 
TACAGTCTCA TCCAGTTCTC CTCTGTGCTC ATCCTCTACA CTGAGAAGAC TAACCTGGGC 
GACCTGCAGT TTCTGTTCTT TGACATGGTC CTGGTGACGC TGCTGGCTAT CGTCATGGGG 
AAAGGGGGCC CAAGCACAGA GCTGCACCCC CAGCGGCCCC CAGCCAGCTT GCTGGCGCTG 
CCAGTCCTGG CTAGCCTTAC GCTGCATACC CTGCTTCTCA TCCTGGGCCA GGTGTCTGCT 
CTGCTGATCA CCACCTCCCA GGACTGGTTT GTTCCTCTCA ATTCAACAAT GTCAGGAGCA 
GCGAACCTGC CCAACCTTGA GGACACCAGT GTGTTTGACC TGTCAGGGTA CCAGTACATC 
ATCATGGCGG TGGTGGTGAC TAAAGGATAC CCCTACAAGA GACCTCTGTA CTACAACGTG 
GTTTTTGTGT GTGTGCTGCT GGTCTTGTTC GCTCTGATGA GTTGGCTGGT GCTAGCTCCC 
GTGCCTTTCC TGCGCAAGCT GCTGAAGCTT TACTACATCC CTGATATGAA CTTCAAAATC 
CTGCTGGTGG CCCTGGCCGC ACTCAACTTT GTCTTCTGCT TCCTATTGGA GATGCTGATA 
GACCGAGGTA TGCTGAACTG CCTGCGTCTG CTCCGAGGGA GGCGCCAGTC CAAGAAGCAG 
TACAAGCGCC TAGACGTCCT CCTGTCAGAT TCTCCCTCCT GGCCCCCCCT GGACCAACCA 
CTAGTCCCTT CCCAGTGCAC AGTCATTGGC CTGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021422065.1
CDS	126..3644
Translation	MTKGRMDTRGCPEEEPPGGFCSPSPRREPHCKPETHMDVQGFRWVRWRVWLCHIGSVLSLGLLLVLFRWRPRLGVLARCNSCPLAFANILLLRDSFGQQYVVEVLTEELEEGSLESAGSEAEDSSDWRDTVQLHKEEVPLLHYYLFEGLRYVWMARKGAFCRVSVLNEDWTCADIYRFREGLSRLEQNTRRKIYGTNLINVPVKSFMRLLIEEVLNPFYVFQVFSIALWLTDSYYYYAACIVIISLISICVSLYEIRKQSNTIRSMAQQITNVTVRRSSGEEDSLSSVELVPGDCVLIPQEGLLLPCDAALLAGECMVNESMLTGESVPVMKTPLPAAGGRYSPETQRRHTLFCGTQCIQAKGGRPGRGGAIAVVTSTGFFTAKGDLVSSILYPQPINFRFYQDAIKFLLFLGGVALCGTVYSIVILSRTNTTWWELVIRALDIVTIVVPPALPAAITTGTIYAQSRLKREGVFCISPPRINISGKVSLFCFDKTGTLTEEGLDVWGVMEGSGAGFSELVPDPRLLPQGPMLSAMACCHSVALLGGQPLGDPLELKMIESTGWELEEPAGDDKTLDSDFGGHRVLAVMRPPACPLQSESVSIHEAVALVQRFPFSSSLQRMSVVTVNPEGQSSLAFIKGAPEMVASLCLRESVPAQFSNTLRTFASDGFRVLALGYKTLYGQTDLSTIERGEVERDMQFLGLLVMKNLVKPESAEVITTLRLAHLRTVMVTGDNVLTAVNVARTCGMLGSNERVIFVHATPQTAQSLPSLRFHLGEGGTEDTATQSSIDMYTQGLYQGVINYHLAINGKSFSALCDHFPEYLPKVLMRGTVYARMAPEQKTQLVTELQKLNYRVGMCGDGANDCGALRAADVGVSLSEAEASVASPFTSKSDNISCVPLLIREGRCSLVTSFSLFKYMALYSLIQFSSVLILYTEKTNLGDLQFLFFDMVLVTLLAIVMGKGGPSTELHPQRPPASLLALPVLASLTLHTLLLILGQVSALLITTSQDWFVPLNSTMSGAANLPNLEDTSVFDLSGYQYIIMAVVVTKGYPYKRPLYYNVVFVCVLLVLFALMSWLVLAPVPFLRKLLKLYYIPDMNFKILLVALAALNFVFCFLLEMLIDRGMLNCLRLLRGRRQSKKQYKRLDVLLSDSPSWPPLDQPLVPSQCTVIGLS

Target ORF information:

RefSeq Version	XM_021566390.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss ATPase 13A2 (atp13a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021566390.1

ORF Insert Sequence:

ATGACTAAAG GAAGGATGGA CACGCGTGGT TGTCCGGAGG AGGAGCCACC GGGAGGGTTC 
TGCTCCCCTA GTCCAAGGAG AGAACCCCAC TGCAAACCCG AAACACACAT GGATGTCCAG 
GGGTTCAGAT GGGTGCGCTG GCGTGTCTGG CTGTGTCACA TTGGGTCCGT GTTGTCCCTG 
GGTCTGTTGC TGGTGCTGTT TCGCTGGCGC CCTCGTCTAG GGGTCCTTGC TCGCTGCAAC 
TCCTGTCCCC TGGCCTTCGC CAATATTCTG CTTCTGAGGG ACAGTTTTGG ACAGCAGTAT 
GTCGTGGAGG TCCTCACAGA AGAGCTGGAG GAGGGCAGTT TGGAGTCGGC AGGGAGTGAG 
GCGGAGGACA GTTCAGACTG GAGAGATACT GTCCAGCTAC ACAAAGAGGA GGTACCGCTG 
CTGCATTACT ACCTGTTTGA GGGGCTGCGC TACGTATGGA TGGCCAGGAA GGGAGCCTTC 
TGTCGTGTTA GTGTCCTCAA TGAGGACTGG ACATGTGCCG ACATTTATCG TTTCCGGGAG 
GGCCTGAGTC GCCTTGAGCA GAACACCAGG AGGAAGATCT ACGGGACCAA TCTCATCAAT 
GTGCCTGTGA AGTCCTTCAT GCGACTACTG ATTGAGGAGG TGCTCAACCC ATTCTACGTG 
TTCCAGGTGT TCAGCATTGC TTTGTGGCTG ACAGATAGTT ACTACTACTA TGCTGCTTGC 
ATCGTCATCA TCTCCCTTAT CTCCATATGT GTGTCTCTCT ACGAGATCCG CAAGCAAAGC 
AACACGATCC GTAGCATGGC TCAGCAGATA ACCAATGTTA CCGTGCGCAG AAGCTCCGGG 
GAGGAGGACA GTTTGAGCTC TGTGGAGCTG GTCCCGGGGG ACTGTGTGCT GATCCCTCAG 
GAGGGGCTGC TGCTGCCCTG CGACGCTGCT CTGCTGGCCG GGGAGTGCAT GGTCAACGAG 
AGTATGCTAA CCGGTGAGAG TGTCCCTGTG ATGAAGACCC CGCTGCCGGC TGCTGGGGGG 
CGTTACAGCC CTGAGACTCA GCGCAGACAC ACCCTCTTCT GCGGCACGCA GTGCATCCAG 
GCCAAAGGGG GCCGGCCAGG AAGGGGCGGG GCCATTGCCG TGGTCACAAG CACAGGGTTC 
TTCACAGCCA AGGGAGACCT GGTCAGCTCC ATCCTCTACC CCCAACCTAT CAACTTCCGC 
TTCTACCAAG ACGCTATTAA GTTCTTGCTT TTCCTGGGGG GTGTAGCATT GTGTGGCACA 
GTGTACAGCA TCGTGATCCT AAGCAGAACC AACACCACGT GGTGGGAGCT GGTGATTCGG 
GCTCTGGATA TTGTGACCAT TGTGGTGCCC CCTGCCCTCC CCGCTGCTAT CACCACCGGT 
ACCATTTACG CCCAGAGTCG TCTGAAGAGA GAAGGGGTCT TCTGCATAAG CCCCCCGCGC 
ATCAACATCT CTGGGAAGGT CTCGCTCTTT TGCTTTGACA AGACAGGCAC TCTAACTGAG 
GAGGGACTGG ACGTGTGGGG GGTGATGGAG GGTAGTGGAG CTGGGTTCTC TGAGCTGGTC 
CCTGACCCAC GCCTCCTGCC CCAGGGGCCC ATGCTCTCTG CAATGGCCTG CTGCCACTCT 
GTGGCCTTGC TGGGGGGACA ACCCCTGGGA GACCCTCTAG AGCTCAAGAT GATTGAGTCC 
ACTGGCTGGG AGCTGGAGGA GCCTGCAGGA GATGACAAAA CGTTAGACTC AGATTTCGGA 
GGTCACAGGG TTCTGGCAGT GATGCGACCC CCTGCCTGTC CGCTCCAATC TGAGAGTGTA 
TCCATCCATG AGGCGGTGGC CCTCGTGCAG CGTTTCCCTT TCTCCTCGTC CCTCCAGCGG 
ATGAGTGTGG TGACGGTGAA TCCTGAGGGA CAGTCGTCCC TCGCCTTTAT CAAAGGGGCC 
CCAGAGATGG TGGCCAGCCT CTGCCTGAGA GAAAGCGTGC CTGCACAATT CTCAAACACC 
CTGCGTACTT TCGCAAGTGA CGGCTTCAGG GTGCTAGCTC TCGGCTACAA AACACTGTAT 
GGGCAGACTG ACTTGAGCAC CATTGAACGG GGAGAGGTAG AGCGAGACAT GCAGTTCCTG 
GGTCTGCTGG TGATGAAGAA TCTGGTCAAG CCGGAGAGTG CCGAGGTCAT AACTACCCTC 
AGATTGGCAC ATCTTCGTAC CGTCATGGTC ACTGGTGACA ACGTTCTGAC TGCAGTCAAT 
GTGGCTCGAA CCTGTGGGAT GTTGGGCTCA AACGAGAGGG TGATTTTTGT TCACGCCACC 
CCCCAGACGG CCCAGTCTCT GCCCTCACTG CGCTTCCACC TGGGGGAAGG AGGGACGGAG 
GACACAGCTA CACAGAGCTC CATAGACATG TACACTCAGG GTCTGTACCA GGGTGTTATC 
AACTATCACC TGGCCATCAA TGGAAAGTCC TTCTCCGCGC TGTGTGACCA CTTCCCTGAG 
TATCTGCCAA AGGTGTTGAT GCGGGGAACG GTCTACGCAC GGATGGCCCC TGAACAGAAG 
ACTCAGCTGG TCACAGAGCT GCAGAAACTG AACTACCGTG TGGGCATGTG CGGAGACGGG 
GCCAACGACT GCGGAGCCCT AAGGGCCGCA GACGTAGGCG TGTCTCTGTC AGAGGCAGAG 
GCATCCGTGG CCTCTCCCTT CACCTCCAAG TCAGACAACA TCAGCTGTGT GCCTCTACTC 
ATCCGGGAGG GCCGGTGTTC TCTGGTGACA TCATTCAGTC TCTTCAAGTA CATGGCCTTG 
TACAGTCTCA TCCAGTTCTC CTCTGTGCTC ATCCTCTACA CTGAGAAGAC TAACCTGGGC 
GACCTGCAGT TTCTGTTCTT TGACATGGTC CTGGTGACGC TGCTGGCTAT CGTCATGGGG 
AAAGGGGGCC CAAGCACAGA GCTGCACCCC CAGCGGCCCC CAGCCAGCTT GCTGGCGCTG 
CCAGTCCTGG CTAGCCTTAC GCTGCATACC CTGCTTCTCA TCCTGGGCCA GGTGTCTGCT 
CTGCTGATCA CCACCTCCCA GGACTGGTTT GTTCCTCTCA ATTCAACAAT GTCAGGAGCA 
GCGAACCTGC CCAACCTTGA GGACACCAGT GTGTTTGACC TGTCAGGGTA CCAGTACATC 
ATCATGGCGG TGGTGGTGAC TAAAGGATAC CCCTACAAGA GACCTCTGTA CTACAACGTG 
GTTTTTGTGT GTGTGCTGCT GGTCTTGTTC GCTCTGATGA GTTGGCTGGT GCTAGCTCCC 
GTGCCTTTCC TGCGCAAGCT GCTGAAGCTT TACTACATCC CTGATATGAA CTTCAAAATC 
CTGCTGGTGG CCCTGGCCGC ACTCAACTTT GTCTTCTGCT TCCTATTGGA GATGCTGATA 
GACCGAGGTA TGCTGAACTG CCTGCGTCTG CTCCGAGGGA GGCGCCAGTC CAAGAAGCAG 
TACAAGCGCC TAGACGTCCT CCTGTCAGAT TCTCCCTCCT GGCCCCCCCT GGACCAACCA 
CTAGTCCCTT CCCAGTGCAC AGTCATTGGC CTGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp13a2 ( XM_021566391.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021422066.1 Oncorhynchus mykiss ATPase 13A2 (atp13a2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf16010
Clone ID Related Accession (Same CDS sequence)	XM_021566391.1
Accession Version	XM_021566391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	cation-transporting ATPase 13A2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAG GAAGGATGGA CACGCGTGGT TGTCCGGAGG AGGAGCCACC GGGAGGGTTC 
TGCTCCCCTA GTCCAAGGAG AGAACCCCAC TGCAAACCCG AAACACACAT GGATGTCCAG 
GGGTTCAGAT GGGTGCGCTG GCGTGTCTGG CTGTGTCACA TTGGGTCCGT GTTGTCCCTG 
GGTCTGTTGC TGGTGCTGTT TCGCTGGCGC CCTCGTCTAG GGGTCCTTGC TCGCTGCAAC 
TCCTGTCCCC TGGCCTTCGC CAATATTCTG CTTCTGAGGG ACAGTTTTGG ACAGCAGTAT 
GTCGTGGAGG TCCTCACAGA AGAGCTGGAG GAGGGCAGTT TGGAGTCGGC AGGGAGTGAG 
GCGGAGGACA GTTCAGACTG GAGAGATACT GTCCAGCTAC ACAAAGAGGA GGTACCGCTG 
CTGCATTACT ACCTGTTTGA GGGGCTGCGC TACGTATGGA TGGCCAGGAA GGGAGCCTTC 
TGTCGTGTTA GTGTCCTCAA TGAGGACTGG ACATGTGCCG ACATTTATCG TTTCCGGGAG 
GGCCTGAGTC GCCTTGAGCA GAACACCAGG AGGAAGATCT ACGGGACCAA TCTCATCAAT 
GTGCCTGTGA AGTCCTTCAT GCGACTACTG ATTGAGGAGG TGTTCAGCAT TGCTTTGTGG 
CTGACAGATA GTTACTACTA CTATGCTGCT TGCATCGTCA TCATCTCCCT TATCTCCATA 
TGTGTGTCTC TCTACGAGAT CCGCAAGCAA AGCAACACGA TCCGTAGCAT GGCTCAGCAG 
ATAACCAATG TTACCGTGCG CAGAAGCTCC GGGGAGGAGG ACAGTTTGAG CTCTGTGGAG 
CTGGTCCCGG GGGACTGTGT GCTGATCCCT CAGGAGGGGC TGCTGCTGCC CTGCGACGCT 
GCTCTGCTGG CCGGGGAGTG CATGGTCAAC GAGAGTATGC TAACCGGTGA GAGTGTCCCT 
GTGATGAAGA CCCCGCTGCC GGCTGCTGGG GGGCGTTACA GCCCTGAGAC TCAGCGCAGA 
CACACCCTCT TCTGCGGCAC GCAGTGCATC CAGGCCAAAG GGGGCCGGCC AGGAAGGGGC 
GGGGCCATTG CCGTGGTCAC AAGCACAGGG TTCTTCACAG CCAAGGGAGA CCTGGTCAGC 
TCCATCCTCT ACCCCCAACC TATCAACTTC CGCTTCTACC AAGACGCTAT TAAGTTCTTG 
CTTTTCCTGG GGGGTGTAGC ATTGTGTGGC ACAGTGTACA GCATCGTGAT CCTAAGCAGA 
ACCAACACCA CGTGGTGGGA GCTGGTGATT CGGGCTCTGG ATATTGTGAC CATTGTGGTG 
CCCCCTGCCC TCCCCGCTGC TATCACCACC GGTACCATTT ACGCCCAGAG TCGTCTGAAG 
AGAGAAGGGG TCTTCTGCAT AAGCCCCCCG CGCATCAACA TCTCTGGGAA GGTCTCGCTC 
TTTTGCTTTG ACAAGACAGG CACTCTAACT GAGGAGGGAC TGGACGTGTG GGGGGTGATG 
GAGGGTAGTG GAGCTGGGTT CTCTGAGCTG GTCCCTGACC CACGCCTCCT GCCCCAGGGG 
CCCATGCTCT CTGCAATGGC CTGCTGCCAC TCTGTGGCCT TGCTGGGGGG ACAACCCCTG 
GGAGACCCTC TAGAGCTCAA GATGATTGAG TCCACTGGCT GGGAGCTGGA GGAGCCTGCA 
GGAGATGACA AAACGTTAGA CTCAGATTTC GGAGGTCACA GGGTTCTGGC AGTGATGCGA 
CCCCCTGCCT GTCCGCTCCA ATCTGAGAGT GTATCCATCC ATGAGGCGGT GGCCCTCGTG 
CAGCGTTTCC CTTTCTCCTC GTCCCTCCAG CGGATGAGTG TGGTGACGGT GAATCCTGAG 
GGACAGTCGT CCCTCGCCTT TATCAAAGGG GCCCCAGAGA TGGTGGCCAG CCTCTGCCTG 
AGAGAAAGCG TGCCTGCACA ATTCTCAAAC ACCCTGCGTA CTTTCGCAAG TGACGGCTTC 
AGGGTGCTAG CTCTCGGCTA CAAAACACTG TATGGGCAGA CTGACTTGAG CACCATTGAA 
CGGGGAGAGG TAGAGCGAGA CATGCAGTTC CTGGGTCTGC TGGTGATGAA GAATCTGGTC 
AAGCCGGAGA GTGCCGAGGT CATAACTACC CTCAGATTGG CACATCTTCG TACCGTCATG 
GTCACTGGTG ACAACGTTCT GACTGCAGTC AATGTGGCTC GAACCTGTGG GATGTTGGGC 
TCAAACGAGA GGGTGATTTT TGTTCACGCC ACCCCCCAGA CGGCCCAGTC TCTGCCCTCA 
CTGCGCTTCC ACCTGGGGGA AGGAGGGACG GAGGACACAG CTACACAGAG CTCCATAGAC 
ATGTACACTC AGGGTCTGTA CCAGGGTGTT ATCAACTATC ACCTGGCCAT CAATGGAAAG 
TCCTTCTCCG CGCTGTGTGA CCACTTCCCT GAGTATCTGC CAAAGGTGTT GATGCGGGGA 
ACGGTCTACG CACGGATGGC CCCTGAACAG AAGACTCAGC TGGTCACAGA GCTGCAGAAA 
CTGAACTACC GTGTGGGCAT GTGCGGAGAC GGGGCCAACG ACTGCGGAGC CCTAAGGGCC 
GCAGACGTAG GCGTGTCTCT GTCAGAGGCA GAGGCATCCG TGGCCTCTCC CTTCACCTCC 
AAGTCAGACA ACATCAGCTG TGTGCCTCTA CTCATCCGGG AGGGCCGGTG TTCTCTGGTG 
ACATCATTCA GTCTCTTCAA GTACATGGCC TTGTACAGTC TCATCCAGTT CTCCTCTGTG 
CTCATCCTCT ACACTGAGAA GACTAACCTG GGCGACCTGC AGTTTCTGTT CTTTGACATG 
GTCCTGGTGA CGCTGCTGGC TATCGTCATG GGGAAAGGGG GCCCAAGCAC AGAGCTGCAC 
CCCCAGCGGC CCCCAGCCAG CTTGCTGGCG CTGCCAGTCC TGGCTAGCCT TACGCTGCAT 
ACCCTGCTTC TCATCCTGGG CCAGGTGTCT GCTCTGCTGA TCACCACCTC CCAGGACTGG 
TTTGTTCCTC TCAATTCAAC AATGTCAGGA GCAGCGAACC TGCCCAACCT TGAGGACACC 
AGTGTGTTTG ACCTGTCAGG GTACCAGTAC ATCATCATGG CGGTGGTGGT GACTAAAGGA 
TACCCCTACA AGAGACCTCT GTACTACAAC GTGGTTTTTG TGTGTGTGCT GCTGGTCTTG 
TTCGCTCTGA TGAGTTGGCT GGTGCTAGCT CCCGTGCCTT TCCTGCGCAA GCTGCTGAAG 
CTTTACTACA TCCCTGATAT GAACTTCAAA ATCCTGCTGG TGGCCCTGGC CGCACTCAAC 
TTTGTCTTCT GCTTCCTATT GGAGATGCTG ATAGACCGAG GTATGCTGAA CTGCCTGCGT 
CTGCTCCGAG GGAGGCGCCA GTCCAAGAAG CAGTACAAGC GCCTAGACGT CCTCCTGTCA 
GATTCTCCCT CCTGGCCCCC CCTGGACCAA CCACTAGTCC CTTCCCAGTG CACAGTCATT 
GGCCTGAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021422066.1
CDS	126..3617
Translation	MTKGRMDTRGCPEEEPPGGFCSPSPRREPHCKPETHMDVQGFRWVRWRVWLCHIGSVLSLGLLLVLFRWRPRLGVLARCNSCPLAFANILLLRDSFGQQYVVEVLTEELEEGSLESAGSEAEDSSDWRDTVQLHKEEVPLLHYYLFEGLRYVWMARKGAFCRVSVLNEDWTCADIYRFREGLSRLEQNTRRKIYGTNLINVPVKSFMRLLIEEVFSIALWLTDSYYYYAACIVIISLISICVSLYEIRKQSNTIRSMAQQITNVTVRRSSGEEDSLSSVELVPGDCVLIPQEGLLLPCDAALLAGECMVNESMLTGESVPVMKTPLPAAGGRYSPETQRRHTLFCGTQCIQAKGGRPGRGGAIAVVTSTGFFTAKGDLVSSILYPQPINFRFYQDAIKFLLFLGGVALCGTVYSIVILSRTNTTWWELVIRALDIVTIVVPPALPAAITTGTIYAQSRLKREGVFCISPPRINISGKVSLFCFDKTGTLTEEGLDVWGVMEGSGAGFSELVPDPRLLPQGPMLSAMACCHSVALLGGQPLGDPLELKMIESTGWELEEPAGDDKTLDSDFGGHRVLAVMRPPACPLQSESVSIHEAVALVQRFPFSSSLQRMSVVTVNPEGQSSLAFIKGAPEMVASLCLRESVPAQFSNTLRTFASDGFRVLALGYKTLYGQTDLSTIERGEVERDMQFLGLLVMKNLVKPESAEVITTLRLAHLRTVMVTGDNVLTAVNVARTCGMLGSNERVIFVHATPQTAQSLPSLRFHLGEGGTEDTATQSSIDMYTQGLYQGVINYHLAINGKSFSALCDHFPEYLPKVLMRGTVYARMAPEQKTQLVTELQKLNYRVGMCGDGANDCGALRAADVGVSLSEAEASVASPFTSKSDNISCVPLLIREGRCSLVTSFSLFKYMALYSLIQFSSVLILYTEKTNLGDLQFLFFDMVLVTLLAIVMGKGGPSTELHPQRPPASLLALPVLASLTLHTLLLILGQVSALLITTSQDWFVPLNSTMSGAANLPNLEDTSVFDLSGYQYIIMAVVVTKGYPYKRPLYYNVVFVCVLLVLFALMSWLVLAPVPFLRKLLKLYYIPDMNFKILLVALAALNFVFCFLLEMLIDRGMLNCLRLLRGRRQSKKQYKRLDVLLSDSPSWPPLDQPLVPSQCTVIGLS

Target ORF information:

RefSeq Version	XM_021566391.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss ATPase 13A2 (atp13a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021566391.1

ORF Insert Sequence:

ATGACTAAAG GAAGGATGGA CACGCGTGGT TGTCCGGAGG AGGAGCCACC GGGAGGGTTC 
TGCTCCCCTA GTCCAAGGAG AGAACCCCAC TGCAAACCCG AAACACACAT GGATGTCCAG 
GGGTTCAGAT GGGTGCGCTG GCGTGTCTGG CTGTGTCACA TTGGGTCCGT GTTGTCCCTG 
GGTCTGTTGC TGGTGCTGTT TCGCTGGCGC CCTCGTCTAG GGGTCCTTGC TCGCTGCAAC 
TCCTGTCCCC TGGCCTTCGC CAATATTCTG CTTCTGAGGG ACAGTTTTGG ACAGCAGTAT 
GTCGTGGAGG TCCTCACAGA AGAGCTGGAG GAGGGCAGTT TGGAGTCGGC AGGGAGTGAG 
GCGGAGGACA GTTCAGACTG GAGAGATACT GTCCAGCTAC ACAAAGAGGA GGTACCGCTG 
CTGCATTACT ACCTGTTTGA GGGGCTGCGC TACGTATGGA TGGCCAGGAA GGGAGCCTTC 
TGTCGTGTTA GTGTCCTCAA TGAGGACTGG ACATGTGCCG ACATTTATCG TTTCCGGGAG 
GGCCTGAGTC GCCTTGAGCA GAACACCAGG AGGAAGATCT ACGGGACCAA TCTCATCAAT 
GTGCCTGTGA AGTCCTTCAT GCGACTACTG ATTGAGGAGG TGTTCAGCAT TGCTTTGTGG 
CTGACAGATA GTTACTACTA CTATGCTGCT TGCATCGTCA TCATCTCCCT TATCTCCATA 
TGTGTGTCTC TCTACGAGAT CCGCAAGCAA AGCAACACGA TCCGTAGCAT GGCTCAGCAG 
ATAACCAATG TTACCGTGCG CAGAAGCTCC GGGGAGGAGG ACAGTTTGAG CTCTGTGGAG 
CTGGTCCCGG GGGACTGTGT GCTGATCCCT CAGGAGGGGC TGCTGCTGCC CTGCGACGCT 
GCTCTGCTGG CCGGGGAGTG CATGGTCAAC GAGAGTATGC TAACCGGTGA GAGTGTCCCT 
GTGATGAAGA CCCCGCTGCC GGCTGCTGGG GGGCGTTACA GCCCTGAGAC TCAGCGCAGA 
CACACCCTCT TCTGCGGCAC GCAGTGCATC CAGGCCAAAG GGGGCCGGCC AGGAAGGGGC 
GGGGCCATTG CCGTGGTCAC AAGCACAGGG TTCTTCACAG CCAAGGGAGA CCTGGTCAGC 
TCCATCCTCT ACCCCCAACC TATCAACTTC CGCTTCTACC AAGACGCTAT TAAGTTCTTG 
CTTTTCCTGG GGGGTGTAGC ATTGTGTGGC ACAGTGTACA GCATCGTGAT CCTAAGCAGA 
ACCAACACCA CGTGGTGGGA GCTGGTGATT CGGGCTCTGG ATATTGTGAC CATTGTGGTG 
CCCCCTGCCC TCCCCGCTGC TATCACCACC GGTACCATTT ACGCCCAGAG TCGTCTGAAG 
AGAGAAGGGG TCTTCTGCAT AAGCCCCCCG CGCATCAACA TCTCTGGGAA GGTCTCGCTC 
TTTTGCTTTG ACAAGACAGG CACTCTAACT GAGGAGGGAC TGGACGTGTG GGGGGTGATG 
GAGGGTAGTG GAGCTGGGTT CTCTGAGCTG GTCCCTGACC CACGCCTCCT GCCCCAGGGG 
CCCATGCTCT CTGCAATGGC CTGCTGCCAC TCTGTGGCCT TGCTGGGGGG ACAACCCCTG 
GGAGACCCTC TAGAGCTCAA GATGATTGAG TCCACTGGCT GGGAGCTGGA GGAGCCTGCA 
GGAGATGACA AAACGTTAGA CTCAGATTTC GGAGGTCACA GGGTTCTGGC AGTGATGCGA 
CCCCCTGCCT GTCCGCTCCA ATCTGAGAGT GTATCCATCC ATGAGGCGGT GGCCCTCGTG 
CAGCGTTTCC CTTTCTCCTC GTCCCTCCAG CGGATGAGTG TGGTGACGGT GAATCCTGAG 
GGACAGTCGT CCCTCGCCTT TATCAAAGGG GCCCCAGAGA TGGTGGCCAG CCTCTGCCTG 
AGAGAAAGCG TGCCTGCACA ATTCTCAAAC ACCCTGCGTA CTTTCGCAAG TGACGGCTTC 
AGGGTGCTAG CTCTCGGCTA CAAAACACTG TATGGGCAGA CTGACTTGAG CACCATTGAA 
CGGGGAGAGG TAGAGCGAGA CATGCAGTTC CTGGGTCTGC TGGTGATGAA GAATCTGGTC 
AAGCCGGAGA GTGCCGAGGT CATAACTACC CTCAGATTGG CACATCTTCG TACCGTCATG 
GTCACTGGTG ACAACGTTCT GACTGCAGTC AATGTGGCTC GAACCTGTGG GATGTTGGGC 
TCAAACGAGA GGGTGATTTT TGTTCACGCC ACCCCCCAGA CGGCCCAGTC TCTGCCCTCA 
CTGCGCTTCC ACCTGGGGGA AGGAGGGACG GAGGACACAG CTACACAGAG CTCCATAGAC 
ATGTACACTC AGGGTCTGTA CCAGGGTGTT ATCAACTATC ACCTGGCCAT CAATGGAAAG 
TCCTTCTCCG CGCTGTGTGA CCACTTCCCT GAGTATCTGC CAAAGGTGTT GATGCGGGGA 
ACGGTCTACG CACGGATGGC CCCTGAACAG AAGACTCAGC TGGTCACAGA GCTGCAGAAA 
CTGAACTACC GTGTGGGCAT GTGCGGAGAC GGGGCCAACG ACTGCGGAGC CCTAAGGGCC 
GCAGACGTAG GCGTGTCTCT GTCAGAGGCA GAGGCATCCG TGGCCTCTCC CTTCACCTCC 
AAGTCAGACA ACATCAGCTG TGTGCCTCTA CTCATCCGGG AGGGCCGGTG TTCTCTGGTG 
ACATCATTCA GTCTCTTCAA GTACATGGCC TTGTACAGTC TCATCCAGTT CTCCTCTGTG 
CTCATCCTCT ACACTGAGAA GACTAACCTG GGCGACCTGC AGTTTCTGTT CTTTGACATG 
GTCCTGGTGA CGCTGCTGGC TATCGTCATG GGGAAAGGGG GCCCAAGCAC AGAGCTGCAC 
CCCCAGCGGC CCCCAGCCAG CTTGCTGGCG CTGCCAGTCC TGGCTAGCCT TACGCTGCAT 
ACCCTGCTTC TCATCCTGGG CCAGGTGTCT GCTCTGCTGA TCACCACCTC CCAGGACTGG 
TTTGTTCCTC TCAATTCAAC AATGTCAGGA GCAGCGAACC TGCCCAACCT TGAGGACACC 
AGTGTGTTTG ACCTGTCAGG GTACCAGTAC ATCATCATGG CGGTGGTGGT GACTAAAGGA 
TACCCCTACA AGAGACCTCT GTACTACAAC GTGGTTTTTG TGTGTGTGCT GCTGGTCTTG 
TTCGCTCTGA TGAGTTGGCT GGTGCTAGCT CCCGTGCCTT TCCTGCGCAA GCTGCTGAAG 
CTTTACTACA TCCCTGATAT GAACTTCAAA ATCCTGCTGG TGGCCCTGGC CGCACTCAAC 
TTTGTCTTCT GCTTCCTATT GGAGATGCTG ATAGACCGAG GTATGCTGAA CTGCCTGCGT 
CTGCTCCGAG GGAGGCGCCA GTCCAAGAAG CAGTACAAGC GCCTAGACGT CCTCCTGTCA 
GATTCTCCCT CCTGGCCCCC CCTGGACCAA CCACTAGTCC CTTCCCAGTG CACAGTCATT 
GGCCTGAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp13a2 ( XM_021566392.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021422067.1 Oncorhynchus mykiss ATPase 13A2 (atp13a2), transcript variant X3, mRNA.

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CloneID	OOf16011
Clone ID Related Accession (Same CDS sequence)	XM_021566392.1
Accession Version	XM_021566392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	cation-transporting ATPase 13A2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTCC AGGGGTTCAG ATGGGTGCGC TGGCGTGTCT GGCTGTGTCA CATTGGGTCC 
GTGTTGTCCC TGGGTCTGTT GCTGGTGCTG TTTCGCTGGC GCCCTCGTCT AGGGGTCCTT 
GCTCGCTGCA ACTCCTGTCC CCTGGCCTTC GCCAATATTC TGCTTCTGAG GGACAGTTTT 
GGACAGCAGT ATGTCGTGGA GGTCCTCACA GAAGAGCTGG AGGAGGGCAG TTTGGAGTCG 
GCAGGGAGTG AGGCGGAGGA CAGTTCAGAC TGGAGAGATA CTGTCCAGCT ACACAAAGAG 
GAGGTACCGC TGCTGCATTA CTACCTGTTT GAGGGGCTGC GCTACGTATG GATGGCCAGG 
AAGGGAGCCT TCTGTCGTGT TAGTGTCCTC AATGAGGACT GGACATGTGC CGACATTTAT 
CGTTTCCGGG AGGGCCTGAG TCGCCTTGAG CAGAACACCA GGAGGAAGAT CTACGGGACC 
AATCTCATCA ATGTGCCTGT GAAGTCCTTC ATGCGACTAC TGATTGAGGA GGTGCTCAAC 
CCATTCTACG TGTTCCAGGT GTTCAGCATT GCTTTGTGGC TGACAGATAG TTACTACTAC 
TATGCTGCTT GCATCGTCAT CATCTCCCTT ATCTCCATAT GTGTGTCTCT CTACGAGATC 
CGCAAGCAAA GCAACACGAT CCGTAGCATG GCTCAGCAGA TAACCAATGT TACCGTGCGC 
AGAAGCTCCG GGGAGGAGGA CAGTTTGAGC TCTGTGGAGC TGGTCCCGGG GGACTGTGTG 
CTGATCCCTC AGGAGGGGCT GCTGCTGCCC TGCGACGCTG CTCTGCTGGC CGGGGAGTGC 
ATGGTCAACG AGAGTATGCT AACCGGTGAG AGTGTCCCTG TGATGAAGAC CCCGCTGCCG 
GCTGCTGGGG GGCGTTACAG CCCTGAGACT CAGCGCAGAC ACACCCTCTT CTGCGGCACG 
CAGTGCATCC AGGCCAAAGG GGGCCGGCCA GGAAGGGGCG GGGCCATTGC CGTGGTCACA 
AGCACAGGGT TCTTCACAGC CAAGGGAGAC CTGGTCAGCT CCATCCTCTA CCCCCAACCT 
ATCAACTTCC GCTTCTACCA AGACGCTATT AAGTTCTTGC TTTTCCTGGG GGGTGTAGCA 
TTGTGTGGCA CAGTGTACAG CATCGTGATC CTAAGCAGAA CCAACACCAC GTGGTGGGAG 
CTGGTGATTC GGGCTCTGGA TATTGTGACC ATTGTGGTGC CCCCTGCCCT CCCCGCTGCT 
ATCACCACCG GTACCATTTA CGCCCAGAGT CGTCTGAAGA GAGAAGGGGT CTTCTGCATA 
AGCCCCCCGC GCATCAACAT CTCTGGGAAG GTCTCGCTCT TTTGCTTTGA CAAGACAGGC 
ACTCTAACTG AGGAGGGACT GGACGTGTGG GGGGTGATGG AGGGTAGTGG AGCTGGGTTC 
TCTGAGCTGG TCCCTGACCC ACGCCTCCTG CCCCAGGGGC CCATGCTCTC TGCAATGGCC 
TGCTGCCACT CTGTGGCCTT GCTGGGGGGA CAACCCCTGG GAGACCCTCT AGAGCTCAAG 
ATGATTGAGT CCACTGGCTG GGAGCTGGAG GAGCCTGCAG GAGATGACAA AACGTTAGAC 
TCAGATTTCG GAGGTCACAG GGTTCTGGCA GTGATGCGAC CCCCTGCCTG TCCGCTCCAA 
TCTGAGAGTG TATCCATCCA TGAGGCGGTG GCCCTCGTGC AGCGTTTCCC TTTCTCCTCG 
TCCCTCCAGC GGATGAGTGT GGTGACGGTG AATCCTGAGG GACAGTCGTC CCTCGCCTTT 
ATCAAAGGGG CCCCAGAGAT GGTGGCCAGC CTCTGCCTGA GAGAAAGCGT GCCTGCACAA 
TTCTCAAACA CCCTGCGTAC TTTCGCAAGT GACGGCTTCA GGGTGCTAGC TCTCGGCTAC 
AAAACACTGT ATGGGCAGAC TGACTTGAGC ACCATTGAAC GGGGAGAGGT AGAGCGAGAC 
ATGCAGTTCC TGGGTCTGCT GGTGATGAAG AATCTGGTCA AGCCGGAGAG TGCCGAGGTC 
ATAACTACCC TCAGATTGGC ACATCTTCGT ACCGTCATGG TCACTGGTGA CAACGTTCTG 
ACTGCAGTCA ATGTGGCTCG AACCTGTGGG ATGTTGGGCT CAAACGAGAG GGTGATTTTT 
GTTCACGCCA CCCCCCAGAC GGCCCAGTCT CTGCCCTCAC TGCGCTTCCA CCTGGGGGAA 
GGAGGGACGG AGGACACAGC TACACAGAGC TCCATAGACA TGTACACTCA GGGTCTGTAC 
CAGGGTGTTA TCAACTATCA CCTGGCCATC AATGGAAAGT CCTTCTCCGC GCTGTGTGAC 
CACTTCCCTG AGTATCTGCC AAAGGTGTTG ATGCGGGGAA CGGTCTACGC ACGGATGGCC 
CCTGAACAGA AGACTCAGCT GGTCACAGAG CTGCAGAAAC TGAACTACCG TGTGGGCATG 
TGCGGAGACG GGGCCAACGA CTGCGGAGCC CTAAGGGCCG CAGACGTAGG CGTGTCTCTG 
TCAGAGGCAG AGGCATCCGT GGCCTCTCCC TTCACCTCCA AGTCAGACAA CATCAGCTGT 
GTGCCTCTAC TCATCCGGGA GGGCCGGTGT TCTCTGGTGA CATCATTCAG TCTCTTCAAG 
TACATGGCCT TGTACAGTCT CATCCAGTTC TCCTCTGTGC TCATCCTCTA CACTGAGAAG 
ACTAACCTGG GCGACCTGCA GTTTCTGTTC TTTGACATGG TCCTGGTGAC GCTGCTGGCT 
ATCGTCATGG GGAAAGGGGG CCCAAGCACA GAGCTGCACC CCCAGCGGCC CCCAGCCAGC 
TTGCTGGCGC TGCCAGTCCT GGCTAGCCTT ACGCTGCATA CCCTGCTTCT CATCCTGGGC 
CAGGTGTCTG CTCTGCTGAT CACCACCTCC CAGGACTGGT TTGTTCCTCT CAATTCAACA 
ATGTCAGGAG CAGCGAACCT GCCCAACCTT GAGGACACCA GTGTGTTTGA CCTGTCAGGG 
TACCAGTACA TCATCATGGC GGTGGTGGTG ACTAAAGGAT ACCCCTACAA GAGACCTCTG 
TACTACAACG TGGTTTTTGT GTGTGTGCTG CTGGTCTTGT TCGCTCTGAT GAGTTGGCTG 
GTGCTAGCTC CCGTGCCTTT CCTGCGCAAG CTGCTGAAGC TTTACTACAT CCCTGATATG 
AACTTCAAAA TCCTGCTGGT GGCCCTGGCC GCACTCAACT TTGTCTTCTG CTTCCTATTG 
GAGATGCTGA TAGACCGAGG TATGCTGAAC TGCCTGCGTC TGCTCCGAGG GAGGCGCCAG 
TCCAAGAAGC AGTACAAGCG CCTAGACGTC CTCCTGTCAG ATTCTCCCTC CTGGCCCCCC 
CTGGACCAAC CACTAGTCCC TTCCCAGTGC ACAGTCATTG GCCTGAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021422067.1
CDS	208..3618
Translation	MDVQGFRWVRWRVWLCHIGSVLSLGLLLVLFRWRPRLGVLARCNSCPLAFANILLLRDSFGQQYVVEVLTEELEEGSLESAGSEAEDSSDWRDTVQLHKEEVPLLHYYLFEGLRYVWMARKGAFCRVSVLNEDWTCADIYRFREGLSRLEQNTRRKIYGTNLINVPVKSFMRLLIEEVLNPFYVFQVFSIALWLTDSYYYYAACIVIISLISICVSLYEIRKQSNTIRSMAQQITNVTVRRSSGEEDSLSSVELVPGDCVLIPQEGLLLPCDAALLAGECMVNESMLTGESVPVMKTPLPAAGGRYSPETQRRHTLFCGTQCIQAKGGRPGRGGAIAVVTSTGFFTAKGDLVSSILYPQPINFRFYQDAIKFLLFLGGVALCGTVYSIVILSRTNTTWWELVIRALDIVTIVVPPALPAAITTGTIYAQSRLKREGVFCISPPRINISGKVSLFCFDKTGTLTEEGLDVWGVMEGSGAGFSELVPDPRLLPQGPMLSAMACCHSVALLGGQPLGDPLELKMIESTGWELEEPAGDDKTLDSDFGGHRVLAVMRPPACPLQSESVSIHEAVALVQRFPFSSSLQRMSVVTVNPEGQSSLAFIKGAPEMVASLCLRESVPAQFSNTLRTFASDGFRVLALGYKTLYGQTDLSTIERGEVERDMQFLGLLVMKNLVKPESAEVITTLRLAHLRTVMVTGDNVLTAVNVARTCGMLGSNERVIFVHATPQTAQSLPSLRFHLGEGGTEDTATQSSIDMYTQGLYQGVINYHLAINGKSFSALCDHFPEYLPKVLMRGTVYARMAPEQKTQLVTELQKLNYRVGMCGDGANDCGALRAADVGVSLSEAEASVASPFTSKSDNISCVPLLIREGRCSLVTSFSLFKYMALYSLIQFSSVLILYTEKTNLGDLQFLFFDMVLVTLLAIVMGKGGPSTELHPQRPPASLLALPVLASLTLHTLLLILGQVSALLITTSQDWFVPLNSTMSGAANLPNLEDTSVFDLSGYQYIIMAVVVTKGYPYKRPLYYNVVFVCVLLVLFALMSWLVLAPVPFLRKLLKLYYIPDMNFKILLVALAALNFVFCFLLEMLIDRGMLNCLRLLRGRRQSKKQYKRLDVLLSDSPSWPPLDQPLVPSQCTVIGLS

Target ORF information:

RefSeq Version	XM_021566392.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss ATPase 13A2 (atp13a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021566392.1

ORF Insert Sequence:

ATGGATGTCC AGGGGTTCAG ATGGGTGCGC TGGCGTGTCT GGCTGTGTCA CATTGGGTCC 
GTGTTGTCCC TGGGTCTGTT GCTGGTGCTG TTTCGCTGGC GCCCTCGTCT AGGGGTCCTT 
GCTCGCTGCA ACTCCTGTCC CCTGGCCTTC GCCAATATTC TGCTTCTGAG GGACAGTTTT 
GGACAGCAGT ATGTCGTGGA GGTCCTCACA GAAGAGCTGG AGGAGGGCAG TTTGGAGTCG 
GCAGGGAGTG AGGCGGAGGA CAGTTCAGAC TGGAGAGATA CTGTCCAGCT ACACAAAGAG 
GAGGTACCGC TGCTGCATTA CTACCTGTTT GAGGGGCTGC GCTACGTATG GATGGCCAGG 
AAGGGAGCCT TCTGTCGTGT TAGTGTCCTC AATGAGGACT GGACATGTGC CGACATTTAT 
CGTTTCCGGG AGGGCCTGAG TCGCCTTGAG CAGAACACCA GGAGGAAGAT CTACGGGACC 
AATCTCATCA ATGTGCCTGT GAAGTCCTTC ATGCGACTAC TGATTGAGGA GGTGCTCAAC 
CCATTCTACG TGTTCCAGGT GTTCAGCATT GCTTTGTGGC TGACAGATAG TTACTACTAC 
TATGCTGCTT GCATCGTCAT CATCTCCCTT ATCTCCATAT GTGTGTCTCT CTACGAGATC 
CGCAAGCAAA GCAACACGAT CCGTAGCATG GCTCAGCAGA TAACCAATGT TACCGTGCGC 
AGAAGCTCCG GGGAGGAGGA CAGTTTGAGC TCTGTGGAGC TGGTCCCGGG GGACTGTGTG 
CTGATCCCTC AGGAGGGGCT GCTGCTGCCC TGCGACGCTG CTCTGCTGGC CGGGGAGTGC 
ATGGTCAACG AGAGTATGCT AACCGGTGAG AGTGTCCCTG TGATGAAGAC CCCGCTGCCG 
GCTGCTGGGG GGCGTTACAG CCCTGAGACT CAGCGCAGAC ACACCCTCTT CTGCGGCACG 
CAGTGCATCC AGGCCAAAGG GGGCCGGCCA GGAAGGGGCG GGGCCATTGC CGTGGTCACA 
AGCACAGGGT TCTTCACAGC CAAGGGAGAC CTGGTCAGCT CCATCCTCTA CCCCCAACCT 
ATCAACTTCC GCTTCTACCA AGACGCTATT AAGTTCTTGC TTTTCCTGGG GGGTGTAGCA 
TTGTGTGGCA CAGTGTACAG CATCGTGATC CTAAGCAGAA CCAACACCAC GTGGTGGGAG 
CTGGTGATTC GGGCTCTGGA TATTGTGACC ATTGTGGTGC CCCCTGCCCT CCCCGCTGCT 
ATCACCACCG GTACCATTTA CGCCCAGAGT CGTCTGAAGA GAGAAGGGGT CTTCTGCATA 
AGCCCCCCGC GCATCAACAT CTCTGGGAAG GTCTCGCTCT TTTGCTTTGA CAAGACAGGC 
ACTCTAACTG AGGAGGGACT GGACGTGTGG GGGGTGATGG AGGGTAGTGG AGCTGGGTTC 
TCTGAGCTGG TCCCTGACCC ACGCCTCCTG CCCCAGGGGC CCATGCTCTC TGCAATGGCC 
TGCTGCCACT CTGTGGCCTT GCTGGGGGGA CAACCCCTGG GAGACCCTCT AGAGCTCAAG 
ATGATTGAGT CCACTGGCTG GGAGCTGGAG GAGCCTGCAG GAGATGACAA AACGTTAGAC 
TCAGATTTCG GAGGTCACAG GGTTCTGGCA GTGATGCGAC CCCCTGCCTG TCCGCTCCAA 
TCTGAGAGTG TATCCATCCA TGAGGCGGTG GCCCTCGTGC AGCGTTTCCC TTTCTCCTCG 
TCCCTCCAGC GGATGAGTGT GGTGACGGTG AATCCTGAGG GACAGTCGTC CCTCGCCTTT 
ATCAAAGGGG CCCCAGAGAT GGTGGCCAGC CTCTGCCTGA GAGAAAGCGT GCCTGCACAA 
TTCTCAAACA CCCTGCGTAC TTTCGCAAGT GACGGCTTCA GGGTGCTAGC TCTCGGCTAC 
AAAACACTGT ATGGGCAGAC TGACTTGAGC ACCATTGAAC GGGGAGAGGT AGAGCGAGAC 
ATGCAGTTCC TGGGTCTGCT GGTGATGAAG AATCTGGTCA AGCCGGAGAG TGCCGAGGTC 
ATAACTACCC TCAGATTGGC ACATCTTCGT ACCGTCATGG TCACTGGTGA CAACGTTCTG 
ACTGCAGTCA ATGTGGCTCG AACCTGTGGG ATGTTGGGCT CAAACGAGAG GGTGATTTTT 
GTTCACGCCA CCCCCCAGAC GGCCCAGTCT CTGCCCTCAC TGCGCTTCCA CCTGGGGGAA 
GGAGGGACGG AGGACACAGC TACACAGAGC TCCATAGACA TGTACACTCA GGGTCTGTAC 
CAGGGTGTTA TCAACTATCA CCTGGCCATC AATGGAAAGT CCTTCTCCGC GCTGTGTGAC 
CACTTCCCTG AGTATCTGCC AAAGGTGTTG ATGCGGGGAA CGGTCTACGC ACGGATGGCC 
CCTGAACAGA AGACTCAGCT GGTCACAGAG CTGCAGAAAC TGAACTACCG TGTGGGCATG 
TGCGGAGACG GGGCCAACGA CTGCGGAGCC CTAAGGGCCG CAGACGTAGG CGTGTCTCTG 
TCAGAGGCAG AGGCATCCGT GGCCTCTCCC TTCACCTCCA AGTCAGACAA CATCAGCTGT 
GTGCCTCTAC TCATCCGGGA GGGCCGGTGT TCTCTGGTGA CATCATTCAG TCTCTTCAAG 
TACATGGCCT TGTACAGTCT CATCCAGTTC TCCTCTGTGC TCATCCTCTA CACTGAGAAG 
ACTAACCTGG GCGACCTGCA GTTTCTGTTC TTTGACATGG TCCTGGTGAC GCTGCTGGCT 
ATCGTCATGG GGAAAGGGGG CCCAAGCACA GAGCTGCACC CCCAGCGGCC CCCAGCCAGC 
TTGCTGGCGC TGCCAGTCCT GGCTAGCCTT ACGCTGCATA CCCTGCTTCT CATCCTGGGC 
CAGGTGTCTG CTCTGCTGAT CACCACCTCC CAGGACTGGT TTGTTCCTCT CAATTCAACA 
ATGTCAGGAG CAGCGAACCT GCCCAACCTT GAGGACACCA GTGTGTTTGA CCTGTCAGGG 
TACCAGTACA TCATCATGGC GGTGGTGGTG ACTAAAGGAT ACCCCTACAA GAGACCTCTG 
TACTACAACG TGGTTTTTGT GTGTGTGCTG CTGGTCTTGT TCGCTCTGAT GAGTTGGCTG 
GTGCTAGCTC CCGTGCCTTT CCTGCGCAAG CTGCTGAAGC TTTACTACAT CCCTGATATG 
AACTTCAAAA TCCTGCTGGT GGCCCTGGCC GCACTCAACT TTGTCTTCTG CTTCCTATTG 
GAGATGCTGA TAGACCGAGG TATGCTGAAC TGCCTGCGTC TGCTCCGAGG GAGGCGCCAG 
TCCAAGAAGC AGTACAAGCG CCTAGACGTC CTCCTGTCAG ATTCTCCCTC CTGGCCCCCC 
CTGGACCAAC CACTAGTCCC TTCCCAGTGC ACAGTCATTG GCCTGAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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atp13a2 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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