LOC100136149 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100136149
Entrez Gene ID	100136149
Full Name	myosin-7
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021562690.1	XP_021418365.1	myosin-7

Pathways from BioSystems
Gene Ontology

The following LOC100136149 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100136149 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf13216	XM_021562690.1 Latest version!	Oncorhynchus mykiss myosin-7 (LOC100136149), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100136149 ( XM_021562690.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021418365.1 Oncorhynchus mykiss myosin-7 (LOC100136149), mRNA.

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CloneID	OOf13216
Clone ID Related Accession (Same CDS sequence)	XM_021562690.1
Accession Version	XM_021562690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	myosin-7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035091.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACA CATTGATGGC AGAGTTTGGC GGTGCAGCTT CCTTTCTGCG TAAATCTGAC 
AAGGAGCGTA TGGAATGCCA GACTAGACCC TTTGACATAA AGAGAGAGTG CTATGTGCCT 
GACCCTGAGG TTGAATACGT CAAGGCCACA ATCACCAGCA GAGATGGGGC TAAAGTCACC 
GCTGAAACAG AGTTTGGAAA GACTGTTACT GTGAAGGAGG ACGACGTCCA CCCCCAGAAC 
CCGCCAAAGT TTGATAAAAT TGAGGACATG GCGATGTTCA CCTTCCTGCA CGAGCCCGCT 
GTGCTGTTTA ACCTCAAAGA GCGTTACGCA GCCTGGATGA TCTACACCTA CTCAGGACTG 
TTCTGTGTCA CTGTCAACCC ATACAAGTGG CTGCCAGTGT ACGATCAGTC TGTTGTCAAT 
GCATACAGAG GCAAGAAGAG GACAGAAGCT CCTCCTCACA TCTTCTCCAT CTCTGACAAT 
GCCTACCAGT ACATGTTGTC AGACAGGGAA AATCAGTCTG TCCTTATCAC TGGAGAATCC 
GGTGCTGGAA AGACTGTTAA CACCAAGAGA GTCATCCAGT ACTTCGCCAG CATTGCTGCT 
GTTGGTGGAA AGAAAAGTGC AGCGGAAGAA AAAAAGGGGA CCCTGGAGGA TCAAATCATC 
CAGGCCAATC CTGCCCTGGA GGCTTTTGGT AATGCCAAGA CCATCAGGAA TGACAACTCC 
TCCCGATTCG GTAAATTCAT CCGAATTCAT TTTGGAGTCA CTGGGAAGCT TTCATCTGCT 
GACATTGAGA CTTATCTTCT GGAGAAGTCA CGTGTCACTT TCCAGCTCAA GGCTGAGAGA 
GACTATCACA TCTTCTACCA GATCCTGTCC CAAAAGAAAC CAGAACTGCT GGAGATGCTA 
CTCATCACAA GCAATCCCTA TGACTATGCC TTCATCTCCC AAGGAGAGAT TGCTGTAACT 
TCCATTAATG ATGCAGATGA GCTAATGGCT ACTGATGATG CCTTTAATGT GCTTGGATTT 
GCCCAAGAAG AGATTAATGG CATTTATAAG CTGACTGGTG CCATCATGCA CTACGGCAAC 
ATGAAGTTCA AGAATAAGCA GCGGGAGGAG CAAGCAGAGG CAGATGGCAC TGAGGATATT 
GACAAAGCTG CATATCTGAT GTGCCTGAAC TCTGCTGACC TAGTCAAGGG GCTGTGTAAC 
CCAAGGGTCA AAGTTGGAAA TGAGTGGGTC ACAAAAGGTC AGAATGTCAA CCAGGTGTAC 
TACGCTGTTG GTGCACTGGC AAAGAAAATT TATGAGAACA TGTTCCTCTG GATGGTGATA 
AGAATCAACC TTACTCTGGA CACTAAGAAT GCTCGCCAGC ACTACATTGG TGTGCTGGAC 
ATTGCTGGCT TTGAGATCTT TGATTTCAAC ACCTTTGAGC AGCTGTGCAT CAACTTCACT 
AATGAGAAGC TGCAGCAGTT CTTCAACCAC CACATGTTTG TGCTGGAGCA GGAAGAGTAT 
AAGAAAGAGG GCATCGTCTG GGAGTTCATT GACTTTGGCA TGGACTTGGC TGCCTGCATT 
GATCTCATTG AAAGGCCCAT GGGTATCATG TCCATCCTTG AAGAGGAGTG CATGTTCCCC 
AAGGCCAGTG ATTCCACATT CAAAGCAAAG CTGTATGAAA ACCATCTGGG CAAAAATGCC 
TGCTTCCAGA AGCCTAGGAT TATTAAGGGT AGACCAGAGG CCCATTTCTC CCTGGTTCAC 
TATGCTGGCA TTGTTGACTA CAACATTGGT AACTGGCTGG TGAAGAACAA GGACCCCCTG 
AATGAGACTG TGGTTGGACT GTTCCAGAAG TCAAGTCTTA AGTTCCTGGC CAACCTATTT 
GCAAACTATG CTGGTGCAGA AGGAGCACCT GAAGAAAAAG CGGCTGGAGG AAAAAAGAAG 
AAAGGCTCTT CCTTCCAGAC TGTGTCTGCA TTGCACAGGG AGAACTTGGG TAAACTCATG 
ACCAACTTGA GGTCTACTCA CCCCCACTTT GTGCGTTGCA TCATCCCCAA CGAGACCAAG 
ACTCCTGGGG CCATGGAGAA TCCTCTGGTC ATGCACCAGC TGCGCTGTAA CGGTGTGCTG 
GAAGGCATCA GGATCTGCAG AAAGGGCTTC CCCAACAGAA TCCTGTATGC TGACTTCAAA 
CAAAGATACC GCATCCTCAA TCCAAGTTCT ATCCCTGAGG GTCAGTTCAT TGACAACATG 
AAGGCAGCTG AAAAACTCCT GGGCTCTCTG GACATTGACC ATACCCAGTA CAGATTAGGA 
CACACTAAGG TGTTCTTCAA GGCTGGTCTC CTGGGTCTAC TTGAGGAGAT GAGAGACGAT 
CGTCTCGCTC TTATCATCAC TGGCTTCCAG GCCAGATCAC GTGGTCTACT TGCTAGAATT 
GAGTTCCAGA AAATGGTTGA CCGCAGGGAT GCCTTGCTTG TGATCCAATG GAACATTCGT 
GCCTTCATGG GTGTCAAGAA TTGGCCCTGG ATGAAGATGT TCTTCAAGAT CAAACCTCTG 
CTGAAGTCAG CAGAGACTGA GAAGGAGATG GCCAACATGA AGGAAGAATT CCTGAAGCTT 
AAAGAGGCTT ATGCTAAATC TGAAGCTCGT AGAAAGGAGC TGGAAGAAAA GATGGTCTCC 
CTTCTCCAAG AGAGGAATGA CCTGCAGCTC GCTGTCCAAA CTTCAGAAGA CACTATTGGT 
GATGCTGAAG AGAGATGTGA GGGTCTGATC AAGAGCAAAA TCCAGCTTGA GGCCAAAGCC 
AAAGAGCTGA CAGAAAGACT GGAAGATGAG GAGGAGATGA ATTCAGAGCT AACTGCTAAG 
AAGAGGAAGC TGGAGGATGA GTGCTCAGAA CTCAAGAAGG ACATTGATGA TCTGGAGCTC 
ACTCTGGCTA AAGTGGAGAA GGAAAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTGACT 
GAGGAAATGG CAGCTCTGGA TGAAATCATT GCCAAGCTGA CCAAGGAGAA GAAGGCTCTG 
CAAGAGGCTC ATCAGCAAAC GCTGGATGAC CTGCAGAGTG AGGAGGACAA AGTCAACACG 
CTGACCAAGG CCAAAGCCAA ACTGGAACAG CAAGTTGATG ATCTTGAAGG GTCTCTGGAG 
CAAGAGAAGA AGGTGAGAAT GGACCTTGAG AGAGCCAAGA GAAAGCTGGA AGGAGACTTG 
AAGTTGACCC AGGAGAGCCT AATGGACCTG GAGAATGACA AGCAGCAGCT GGAGGAGAGA 
ATGAAGAAGA AGGACTTTGA GATGAGCCAA CTCAACAGCA AGATTGAGGA TGAGCAGGCC 
ATGAGTGCCC AGCTTCAGAA GAAACTGAAG GAGCTGCAGG CCCGCATTGA GGAATTAGAG 
GAAGAGCTGG AGGCTGAAAG AGCTGCCCGT GCCAAGGTGG AGAAACAGAG GGCAGACTTG 
TCCAGAGAGC TAGAAGAGAT CAGTGAGAGG CTGGAGGAGG CTGGTGGAGC CACTGCTGCC 
CAGATTGAGA TGAACAAGAA GAGGGAGGCT GAGTTCCAGA AGGTGCGCAG AGACCTTGAA 
GAGGCTACCC TGCAGCATGA GGCTACAGCT GCCACTCTGA GGAAGAAAAA TGCTGACAGT 
GTAGCTGACC TGGGAGAGCA GATTGACAAC CTTCAGAGAG TGAAGCAGAA GCTGGAGAAA 
GAGAAGAGTG AGCTCAGGCT GGAGCTGGAT GATGTGGTCT CCAACATGGA GCAGATTGTC 
AAGTCCAAAA CAAACCTGGA GAAAATGTGC CGCACTCTAG AGGACCAGAT GAGTGAATAC 
AGGACAAAAG CTGAGGAAGG ACAGCGATCC ATCAATGATT TCACCATGCA GAAAGCAAAG 
CTTCAAACTG AGAATGGTGA ATTTGCCAGA CAGTTGGAAG AGAAGGACTC TCTGGTCTCT 
CAACTGACCA GAGGTAAGCA GTCCAATGTT CAGCAGATTG AAGACCTCAA GAGACAACTA 
GAGGAGGAAG TCAAGGCCAA GAACGCACTT GCCCATGCAG TGCAGTCTGC TCGCCATGAC 
TCAGATCTGC TGAGGGAGCA GTATGAGGAG GAGCAGGAGG CCAAGTCTGA GCTGCAGCGT 
GGCATGTCCA AGGCTAATGC TGAGGTGGCT CAGTGGAGAA CCAAGTATGA AACTGATGCC 
ATCCAGAAGA CTGAGGAGCT TGAGGACGCA AAGAAGAAGC TGGCTCAGCG TCTGCAGGAT 
GCAGAGGAAG CTGTGGAAGC TGTAAATGCT AAATGTTCAT CCCTGGAGAA GACTAAACAC 
AGACTCCAGA ATGAGATTGA AGATCTCATG GTGGATGTGG AGAGATCTAA TGCAGCTGCT 
GCCTCTCTGG ACAAGAAGCA AAGGAACTTT GACAAGATCC TGGCTGACTG GAAGCAGAAG 
TTTGAGGAGT CTCAGACTGA GCTGGAGAGC TCCCAGAAAG AGGCCAGATC TCTCAGCACT 
GAGCTCTTCA AACTCAAGAA CTCGTATGAG GAGTCTCTGG ATCATCTGGA GACCATGAAG 
AGGGAGAACA AGAACCTCCA AGAGGAAATT TCTGACCTGA CGGAGCAGCT TGGTGAGGGA 
GGAAAGAGCA TCCATGAACT GGAGAAGATC CGTAAACAGC TGGAGCAGGA GAAGGCTGAG 
ATACAGTCTG CTCTAGAGGA AGCTGAGGCC TCCCTAGAGC ATGAGGAAGG CAAGATCATG 
AGAGCTCAGC TGGAGTTCAA TCAGGTGAAA TCTGACATTG AACGGAAGCT GGTGGAGAAG 
GATGAGGAAA TGGAGATGAA TAAGAGGAAC CAGCAGAGAG TTGTGGATAC CCTGCAAAGC 
TCCCTGGAGT CAGAGACTCG CAGCAGGAAT GAAGCTCTCA GGCTGAAGAA GAAGATGGAG 
GGAGATCTCA ATGAGATGGA GATCCAGCTC AGCCAGGCCA ACAGGCAGGC ATCAGAGGCC 
CAGAAGCAAC TTAAGGGTCT CCATTCCCAT CTGAAGGATT CTCAAATGCA GCTGGATGAT 
GCTCTTCGTA GCAATGATGA CCTGAAGGAG AACATTGCCA TTGTGGAGAG ACGTAACAAC 
TTGATTCAGG CTGAACTGGA TGAGCTGAGA GCCCTGGTGG AGCAGACTGA GAGAGGCCGC 
AAACTGGCTG AGCAGGAACT GTTGGATGTT AGTGAGAGAG TTCAGCTGTT GCACTCACAG 
AACACCAGCT TACTGAGCCA GAAGAAGAAG CTAGAGGGCG ACACATCCCA GCTTCAGAAT 
GAAGTGGAGG AGGCTGTGCA GGAGTGTAGA AATGCAGAGG AAAAGGCCAA GAAGGCCATT 
ACTGATGCTG CCATGATGGC AGAAGAGCTG AAGAAGGAGC AGGACACCAG TGCTCACCTG 
GAGCGCATGA AAAAGAACAT GGAGCAGACC ATCAAGGACC TGCAGCACCG CCTGGATGAA 
GCTGAACAAA TCGCCATGAA GGGTGGCAAG AAGCAGATCC AGAAGCTGGA GGCCAGAGTT 
AGAGAGCTAG AGACTGAAGT GGAACTGGAG CAAAGGAGGA GCAGTGATTC TGTGAAAGGA 
GTCCGTAAAT ATGAGAGACG CATCAAAGAA CTCACCTACC AGACTGAGGA AGACCGTAAG 
AATTTGAGCC GCCTGCAGGA CCTGGTGGAC AAACTGCAGC TGAAGGTCAA ATCCTACAAG 
AGAACTTCAG AGGAGGCTGA GGAACAAGCC AATTCCAATT TGGGCAAGTT CCGTAAGATT 
CAGCATGAAC TGGATGAGGC AGAAGAGAGG GCTGATATTG CTGAGTCTCA GGTCAACAAG 
ATGAGAGCCA AGAGTCGTGA TGCCGGTTCC AAGAAAGGAA AGGATGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021418365.1
CDS	120..5933
Translation	MGDTLMAEFGGAASFLRKSDKERMECQTRPFDIKRECYVPDPEVEYVKATITSRDGAKVTAETEFGKTVTVKEDDVHPQNPPKFDKIEDMAMFTFLHEPAVLFNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYDQSVVNAYRGKKRTEAPPHIFSISDNAYQYMLSDRENQSVLITGESGAGKTVNTKRVIQYFASIAAVGGKKSAAEEKKGTLEDQIIQANPALEAFGNAKTIRNDNSSRFGKFIRIHFGVTGKLSSADIETYLLEKSRVTFQLKAERDYHIFYQILSQKKPELLEMLLITSNPYDYAFISQGEIAVTSINDADELMATDDAFNVLGFAQEEINGIYKLTGAIMHYGNMKFKNKQREEQAEADGTEDIDKAAYLMCLNSADLVKGLCNPRVKVGNEWVTKGQNVNQVYYAVGALAKKIYENMFLWMVIRINLTLDTKNARQHYIGVLDIAGFEIFDFNTFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIDLIERPMGIMSILEEECMFPKASDSTFKAKLYENHLGKNACFQKPRIIKGRPEAHFSLVHYAGIVDYNIGNWLVKNKDPLNETVVGLFQKSSLKFLANLFANYAGAEGAPEEKAAGGKKKKGSSFQTVSALHRENLGKLMTNLRSTHPHFVRCIIPNETKTPGAMENPLVMHQLRCNGVLEGIRICRKGFPNRILYADFKQRYRILNPSSIPEGQFIDNMKAAEKLLGSLDIDHTQYRLGHTKVFFKAGLLGLLEEMRDDRLALIITGFQARSRGLLARIEFQKMVDRRDALLVIQWNIRAFMGVKNWPWMKMFFKIKPLLKSAETEKEMANMKEEFLKLKEAYAKSEARRKELEEKMVSLLQERNDLQLAVQTSEDTIGDAEERCEGLIKSKIQLEAKAKELTERLEDEEEMNSELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDEIIAKLTKEKKALQEAHQQTLDDLQSEEDKVNTLTKAKAKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESLMDLENDKQQLEERMKKKDFEMSQLNSKIEDEQAMSAQLQKKLKELQARIEELEEELEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKVRRDLEEATLQHEATAATLRKKNADSVADLGEQIDNLQRVKQKLEKEKSELRLELDDVVSNMEQIVKSKTNLEKMCRTLEDQMSEYRTKAEEGQRSINDFTMQKAKLQTENGEFARQLEEKDSLVSQLTRGKQSNVQQIEDLKRQLEEEVKAKNALAHAVQSARHDSDLLREQYEEEQEAKSELQRGMSKANAEVAQWRTKYETDAIQKTEELEDAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAASLDKKQRNFDKILADWKQKFEESQTELESSQKEARSLSTELFKLKNSYEESLDHLETMKRENKNLQEEISDLTEQLGEGGKSIHELEKIRKQLEQEKAEIQSALEEAEASLEHEEGKIMRAQLEFNQVKSDIERKLVEKDEEMEMNKRNQQRVVDTLQSSLESETRSRNEALRLKKKMEGDLNEMEIQLSQANRQASEAQKQLKGLHSHLKDSQMQLDDALRSNDDLKENIAIVERRNNLIQAELDELRALVEQTERGRKLAEQELLDVSERVQLLHSQNTSLLSQKKKLEGDTSQLQNEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIAMKGGKKQIQKLEARVRELETEVELEQRRSSDSVKGVRKYERRIKELTYQTEEDRKNLSRLQDLVDKLQLKVKSYKRTSEEAEEQANSNLGKFRKIQHELDEAEERADIAESQVNKMRAKSRDAGSKKGKDEE

Target ORF information:

RefSeq Version	XM_021562690.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss myosin-7 (LOC100136149), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021562690.1

ORF Insert Sequence:

ATGGGGGACA CATTGATGGC AGAGTTTGGC GGTGCAGCTT CCTTTCTGCG TAAATCTGAC 
AAGGAGCGTA TGGAATGCCA GACTAGACCC TTTGACATAA AGAGAGAGTG CTATGTGCCT 
GACCCTGAGG TTGAATACGT CAAGGCCACA ATCACCAGCA GAGATGGGGC TAAAGTCACC 
GCTGAAACAG AGTTTGGAAA GACTGTTACT GTGAAGGAGG ACGACGTCCA CCCCCAGAAC 
CCGCCAAAGT TTGATAAAAT TGAGGACATG GCGATGTTCA CCTTCCTGCA CGAGCCCGCT 
GTGCTGTTTA ACCTCAAAGA GCGTTACGCA GCCTGGATGA TCTACACCTA CTCAGGACTG 
TTCTGTGTCA CTGTCAACCC ATACAAGTGG CTGCCAGTGT ACGATCAGTC TGTTGTCAAT 
GCATACAGAG GCAAGAAGAG GACAGAAGCT CCTCCTCACA TCTTCTCCAT CTCTGACAAT 
GCCTACCAGT ACATGTTGTC AGACAGGGAA AATCAGTCTG TCCTTATCAC TGGAGAATCC 
GGTGCTGGAA AGACTGTTAA CACCAAGAGA GTCATCCAGT ACTTCGCCAG CATTGCTGCT 
GTTGGTGGAA AGAAAAGTGC AGCGGAAGAA AAAAAGGGGA CCCTGGAGGA TCAAATCATC 
CAGGCCAATC CTGCCCTGGA GGCTTTTGGT AATGCCAAGA CCATCAGGAA TGACAACTCC 
TCCCGATTCG GTAAATTCAT CCGAATTCAT TTTGGAGTCA CTGGGAAGCT TTCATCTGCT 
GACATTGAGA CTTATCTTCT GGAGAAGTCA CGTGTCACTT TCCAGCTCAA GGCTGAGAGA 
GACTATCACA TCTTCTACCA GATCCTGTCC CAAAAGAAAC CAGAACTGCT GGAGATGCTA 
CTCATCACAA GCAATCCCTA TGACTATGCC TTCATCTCCC AAGGAGAGAT TGCTGTAACT 
TCCATTAATG ATGCAGATGA GCTAATGGCT ACTGATGATG CCTTTAATGT GCTTGGATTT 
GCCCAAGAAG AGATTAATGG CATTTATAAG CTGACTGGTG CCATCATGCA CTACGGCAAC 
ATGAAGTTCA AGAATAAGCA GCGGGAGGAG CAAGCAGAGG CAGATGGCAC TGAGGATATT 
GACAAAGCTG CATATCTGAT GTGCCTGAAC TCTGCTGACC TAGTCAAGGG GCTGTGTAAC 
CCAAGGGTCA AAGTTGGAAA TGAGTGGGTC ACAAAAGGTC AGAATGTCAA CCAGGTGTAC 
TACGCTGTTG GTGCACTGGC AAAGAAAATT TATGAGAACA TGTTCCTCTG GATGGTGATA 
AGAATCAACC TTACTCTGGA CACTAAGAAT GCTCGCCAGC ACTACATTGG TGTGCTGGAC 
ATTGCTGGCT TTGAGATCTT TGATTTCAAC ACCTTTGAGC AGCTGTGCAT CAACTTCACT 
AATGAGAAGC TGCAGCAGTT CTTCAACCAC CACATGTTTG TGCTGGAGCA GGAAGAGTAT 
AAGAAAGAGG GCATCGTCTG GGAGTTCATT GACTTTGGCA TGGACTTGGC TGCCTGCATT 
GATCTCATTG AAAGGCCCAT GGGTATCATG TCCATCCTTG AAGAGGAGTG CATGTTCCCC 
AAGGCCAGTG ATTCCACATT CAAAGCAAAG CTGTATGAAA ACCATCTGGG CAAAAATGCC 
TGCTTCCAGA AGCCTAGGAT TATTAAGGGT AGACCAGAGG CCCATTTCTC CCTGGTTCAC 
TATGCTGGCA TTGTTGACTA CAACATTGGT AACTGGCTGG TGAAGAACAA GGACCCCCTG 
AATGAGACTG TGGTTGGACT GTTCCAGAAG TCAAGTCTTA AGTTCCTGGC CAACCTATTT 
GCAAACTATG CTGGTGCAGA AGGAGCACCT GAAGAAAAAG CGGCTGGAGG AAAAAAGAAG 
AAAGGCTCTT CCTTCCAGAC TGTGTCTGCA TTGCACAGGG AGAACTTGGG TAAACTCATG 
ACCAACTTGA GGTCTACTCA CCCCCACTTT GTGCGTTGCA TCATCCCCAA CGAGACCAAG 
ACTCCTGGGG CCATGGAGAA TCCTCTGGTC ATGCACCAGC TGCGCTGTAA CGGTGTGCTG 
GAAGGCATCA GGATCTGCAG AAAGGGCTTC CCCAACAGAA TCCTGTATGC TGACTTCAAA 
CAAAGATACC GCATCCTCAA TCCAAGTTCT ATCCCTGAGG GTCAGTTCAT TGACAACATG 
AAGGCAGCTG AAAAACTCCT GGGCTCTCTG GACATTGACC ATACCCAGTA CAGATTAGGA 
CACACTAAGG TGTTCTTCAA GGCTGGTCTC CTGGGTCTAC TTGAGGAGAT GAGAGACGAT 
CGTCTCGCTC TTATCATCAC TGGCTTCCAG GCCAGATCAC GTGGTCTACT TGCTAGAATT 
GAGTTCCAGA AAATGGTTGA CCGCAGGGAT GCCTTGCTTG TGATCCAATG GAACATTCGT 
GCCTTCATGG GTGTCAAGAA TTGGCCCTGG ATGAAGATGT TCTTCAAGAT CAAACCTCTG 
CTGAAGTCAG CAGAGACTGA GAAGGAGATG GCCAACATGA AGGAAGAATT CCTGAAGCTT 
AAAGAGGCTT ATGCTAAATC TGAAGCTCGT AGAAAGGAGC TGGAAGAAAA GATGGTCTCC 
CTTCTCCAAG AGAGGAATGA CCTGCAGCTC GCTGTCCAAA CTTCAGAAGA CACTATTGGT 
GATGCTGAAG AGAGATGTGA GGGTCTGATC AAGAGCAAAA TCCAGCTTGA GGCCAAAGCC 
AAAGAGCTGA CAGAAAGACT GGAAGATGAG GAGGAGATGA ATTCAGAGCT AACTGCTAAG 
AAGAGGAAGC TGGAGGATGA GTGCTCAGAA CTCAAGAAGG ACATTGATGA TCTGGAGCTC 
ACTCTGGCTA AAGTGGAGAA GGAAAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTGACT 
GAGGAAATGG CAGCTCTGGA TGAAATCATT GCCAAGCTGA CCAAGGAGAA GAAGGCTCTG 
CAAGAGGCTC ATCAGCAAAC GCTGGATGAC CTGCAGAGTG AGGAGGACAA AGTCAACACG 
CTGACCAAGG CCAAAGCCAA ACTGGAACAG CAAGTTGATG ATCTTGAAGG GTCTCTGGAG 
CAAGAGAAGA AGGTGAGAAT GGACCTTGAG AGAGCCAAGA GAAAGCTGGA AGGAGACTTG 
AAGTTGACCC AGGAGAGCCT AATGGACCTG GAGAATGACA AGCAGCAGCT GGAGGAGAGA 
ATGAAGAAGA AGGACTTTGA GATGAGCCAA CTCAACAGCA AGATTGAGGA TGAGCAGGCC 
ATGAGTGCCC AGCTTCAGAA GAAACTGAAG GAGCTGCAGG CCCGCATTGA GGAATTAGAG 
GAAGAGCTGG AGGCTGAAAG AGCTGCCCGT GCCAAGGTGG AGAAACAGAG GGCAGACTTG 
TCCAGAGAGC TAGAAGAGAT CAGTGAGAGG CTGGAGGAGG CTGGTGGAGC CACTGCTGCC 
CAGATTGAGA TGAACAAGAA GAGGGAGGCT GAGTTCCAGA AGGTGCGCAG AGACCTTGAA 
GAGGCTACCC TGCAGCATGA GGCTACAGCT GCCACTCTGA GGAAGAAAAA TGCTGACAGT 
GTAGCTGACC TGGGAGAGCA GATTGACAAC CTTCAGAGAG TGAAGCAGAA GCTGGAGAAA 
GAGAAGAGTG AGCTCAGGCT GGAGCTGGAT GATGTGGTCT CCAACATGGA GCAGATTGTC 
AAGTCCAAAA CAAACCTGGA GAAAATGTGC CGCACTCTAG AGGACCAGAT GAGTGAATAC 
AGGACAAAAG CTGAGGAAGG ACAGCGATCC ATCAATGATT TCACCATGCA GAAAGCAAAG 
CTTCAAACTG AGAATGGTGA ATTTGCCAGA CAGTTGGAAG AGAAGGACTC TCTGGTCTCT 
CAACTGACCA GAGGTAAGCA GTCCAATGTT CAGCAGATTG AAGACCTCAA GAGACAACTA 
GAGGAGGAAG TCAAGGCCAA GAACGCACTT GCCCATGCAG TGCAGTCTGC TCGCCATGAC 
TCAGATCTGC TGAGGGAGCA GTATGAGGAG GAGCAGGAGG CCAAGTCTGA GCTGCAGCGT 
GGCATGTCCA AGGCTAATGC TGAGGTGGCT CAGTGGAGAA CCAAGTATGA AACTGATGCC 
ATCCAGAAGA CTGAGGAGCT TGAGGACGCA AAGAAGAAGC TGGCTCAGCG TCTGCAGGAT 
GCAGAGGAAG CTGTGGAAGC TGTAAATGCT AAATGTTCAT CCCTGGAGAA GACTAAACAC 
AGACTCCAGA ATGAGATTGA AGATCTCATG GTGGATGTGG AGAGATCTAA TGCAGCTGCT 
GCCTCTCTGG ACAAGAAGCA AAGGAACTTT GACAAGATCC TGGCTGACTG GAAGCAGAAG 
TTTGAGGAGT CTCAGACTGA GCTGGAGAGC TCCCAGAAAG AGGCCAGATC TCTCAGCACT 
GAGCTCTTCA AACTCAAGAA CTCGTATGAG GAGTCTCTGG ATCATCTGGA GACCATGAAG 
AGGGAGAACA AGAACCTCCA AGAGGAAATT TCTGACCTGA CGGAGCAGCT TGGTGAGGGA 
GGAAAGAGCA TCCATGAACT GGAGAAGATC CGTAAACAGC TGGAGCAGGA GAAGGCTGAG 
ATACAGTCTG CTCTAGAGGA AGCTGAGGCC TCCCTAGAGC ATGAGGAAGG CAAGATCATG 
AGAGCTCAGC TGGAGTTCAA TCAGGTGAAA TCTGACATTG AACGGAAGCT GGTGGAGAAG 
GATGAGGAAA TGGAGATGAA TAAGAGGAAC CAGCAGAGAG TTGTGGATAC CCTGCAAAGC 
TCCCTGGAGT CAGAGACTCG CAGCAGGAAT GAAGCTCTCA GGCTGAAGAA GAAGATGGAG 
GGAGATCTCA ATGAGATGGA GATCCAGCTC AGCCAGGCCA ACAGGCAGGC ATCAGAGGCC 
CAGAAGCAAC TTAAGGGTCT CCATTCCCAT CTGAAGGATT CTCAAATGCA GCTGGATGAT 
GCTCTTCGTA GCAATGATGA CCTGAAGGAG AACATTGCCA TTGTGGAGAG ACGTAACAAC 
TTGATTCAGG CTGAACTGGA TGAGCTGAGA GCCCTGGTGG AGCAGACTGA GAGAGGCCGC 
AAACTGGCTG AGCAGGAACT GTTGGATGTT AGTGAGAGAG TTCAGCTGTT GCACTCACAG 
AACACCAGCT TACTGAGCCA GAAGAAGAAG CTAGAGGGCG ACACATCCCA GCTTCAGAAT 
GAAGTGGAGG AGGCTGTGCA GGAGTGTAGA AATGCAGAGG AAAAGGCCAA GAAGGCCATT 
ACTGATGCTG CCATGATGGC AGAAGAGCTG AAGAAGGAGC AGGACACCAG TGCTCACCTG 
GAGCGCATGA AAAAGAACAT GGAGCAGACC ATCAAGGACC TGCAGCACCG CCTGGATGAA 
GCTGAACAAA TCGCCATGAA GGGTGGCAAG AAGCAGATCC AGAAGCTGGA GGCCAGAGTT 
AGAGAGCTAG AGACTGAAGT GGAACTGGAG CAAAGGAGGA GCAGTGATTC TGTGAAAGGA 
GTCCGTAAAT ATGAGAGACG CATCAAAGAA CTCACCTACC AGACTGAGGA AGACCGTAAG 
AATTTGAGCC GCCTGCAGGA CCTGGTGGAC AAACTGCAGC TGAAGGTCAA ATCCTACAAG 
AGAACTTCAG AGGAGGCTGA GGAACAAGCC AATTCCAATT TGGGCAAGTT CCGTAAGATT 
CAGCATGAAC TGGATGAGGC AGAAGAGAGG GCTGATATTG CTGAGTCTCA GGTCAACAAG 
ATGAGAGCCA AGAGTCGTGA TGCCGGTTCC AAGAAAGGAA AGGATGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100136149 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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