rln3b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rln3b
Entrez Gene ID	796288
Full Name	relaxin 3b
Synonyms	rflc1
General protein information	Preferred Names relaxin 3b Names relaxin-3 relaxin family locus C type 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021475846.1	XP_021331521.1	relaxin-3 isoform X1
NM_001115063.1	NP_001108535.1	relaxin-3 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Japanese medaka as a model for studying the relaxin family genes involved in neuroendocrine regulation: Insights from the expression of fish-specific rln3 and insl5 and rxfp3/4-type receptor paralogues.
Alnafea H, Vahkal B, Zelmer CK, Yegorov S, Bogerd J, Good SV
Molecular and cellular endocrinology4872-11(2019 05)

Characterization, cDNA cloning and expression pattern of relaxin gene during embryogenesis of Danio rerio.
Fiengo M, Donizetti A, del Gaudio R, Minucci S, Aniello F
Development, growth & differentiation54(5)579-87(2012 Jun)

New insights into ligand-receptor pairing and coevolution of relaxin family peptides and their receptors in teleosts.
Good S, Yegorov S, Martijn J, Franck J, Bogerd J
International journal of evolutionary biology2012310278(2012)

Relaxin gene family in teleosts: phylogeny, syntenic mapping, selective constraint, and expression analysis.
Good-Avila SV, Yegorov S, Harron S, Bogerd J, Glen P, Ozon J, Wilson BC
BMC evolutionary biology9293(2009 Dec)

Duplicated zebrafish relaxin-3 gene shows a different expression pattern from that of the co-orthologue gene.
Donizetti A, Fiengo M, Minucci S, Aniello F
Development, growth & differentiation51(8)715-22(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rln3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rln3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa04519	NM_001115063.1 Latest version!	Danio rerio relaxin 3b (rln3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa147290	XM_021475846.1 Latest version!	Danio rerio relaxin 3b (rln3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rln3b ( NM_001115063.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001108535.1 Danio rerio relaxin 3b (rln3b), mRNA.

Price & Availability↑

CloneID	ODa04519
Clone ID Related Accession (Same CDS sequence)	NM_001115063.1
Accession Version	NM_001115063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Danio rerio(zebrafish)
Product	relaxin-3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU437453.1. On Mar 2, 2008 this sequence version replaced XM_001336563.1. ##Evidence-Data-START## Transcript exon combination :: EU437453.1 [ECO:0000332] ##Evidence-Data-END##

ATGTGTAGAG TTGCAGTACT CGTGTTTGGC CTGCTGGTTG CGCTGCTGGG CGGCGCTCAG 
GCCACAGATG GACACTCGAT CTACGGCGTG AAGCTCTGCG GGAGAGAGTT CATCCGGGCG 
GTGATCTTCA CCTGCGGAGG CTCGCGCTGG AGGAGATCTC TGGATGATTC AGGTAGGGTA 
AATGATCTGC TCTCCGCTCA TGATGACGAG GCCTCTGAAA GATGGGCCTT AAACTCAGTC 
ATCCCTGGAC TATCATACAG ACCCCGTCCC AACTCGGAGA CCCCTAGCTG GGCAGCAGAG 
GCTTCGGAGG GCCCTGTGTT CAGCCGTACC GCCCGGTCGG TCATCTCAGA GGAAGTGCTG 
GAGGCCCTGC GCACGTCAGA CAGGAAAGGC CGTGATGTCG TGGTCGGCCT GTCCAACGCC 
TGCTGCAAAT GGGGCTGCAG CAAGAGCGAG ATCAGCTCAT TATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108535.1
CDS	1..468
Translation	MCRVAVLVFGLLVALLGGAQATDGHSIYGVKLCGREFIRAVIFTCGGSRWRRSLDDSGRVNDLLSAHDDEASERWALNSVIPGLSYRPRPNSETPSWAAEASEGPVFSRTARSVISEEVLEALRTSDRKGRDVVVGLSNACCKWGCSKSEISSLC

Target ORF information:

RefSeq Version	NM_001115063.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio relaxin 3b (rln3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001115063.1

ORF Insert Sequence:

ATGTGTAGAG TTGCAGTACT CGTGTTTGGC CTGCTGGTTG CGCTGCTGGG CGGCGCTCAG 
GCCACAGATG GACACTCGAT CTACGGCGTG AAGCTCTGCG GGAGAGAGTT CATCCGGGCG 
GTGATCTTCA CCTGCGGAGG CTCGCGCTGG AGGAGATCTC TGGATGATTC AGGTAGGGTA 
AATGATCTGC TCTCCGCTCA TGATGACGAG GCCTCTGAAA GATGGGCCTT AAACTCAGTC 
ATCCCTGGAC TATCATACAG ACCCCGTCCC AACTCGGAGA CCCCTAGCTG GGCAGCAGAG 
GCTTCGGAGG GCCCTGTGTT CAGCCGTACC GCCCGGTCGG TCATCTCAGA GGAAGTGCTG 
GAGGCCCTGC GCACGTCAGA CAGGAAAGGC CGTGATGTCG TGGTCGGCCT GTCCAACGCC 
TGCTGCAAAT GGGGCTGCAG CAAGAGCGAG ATCAGCTCAT TATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rln3b ( XM_021475846.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331521.1 Danio rerio relaxin 3b (rln3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147290
Clone ID Related Accession (Same CDS sequence)	XM_021475846.1
Accession Version	XM_021475846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	relaxin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGAG TTGCAGTACT CGTGTTTGGC CTGCTGGTTG CGCTGCTGGG CGGCGCTCAG 
GCCACAGATG GACACTCGAT CTACGGCGTG AAGCTCTGCG GGAGAGAGTT CATCCGGGCG 
GTGATCTTCA CCTGCGGAGG CTCGCGCTGG AGGAGATCTC TGGATGATTC AGGTGATCTG 
TCCAGTGATC TGCTCTCCGC TCATGATGAC GAGGCCTCTG AAAGATGGGC CTTAAACTCA 
GTCATCCCTG GACTATCATA CAGACCCCGT CCCAACTCGG AGACCCCTAG CTGGGCAGCA 
GAGGCTTCGG AGGGCCCTGT GTTCAGCCGT ACCGCCCGGT CGGTCATCTC AGAGGAAGTG 
CTGGAGGCCC TGCGCACGTC AGACAGGAAA GGCCGTGATG TCGTGGTCGG CCTGTCCAAC 
GCCTGCTGCA AATGGGGCTG CAGCAAGAGC GAGATCAGCT CATTATGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331521.1
CDS	386..856
Translation	MCRVAVLVFGLLVALLGGAQATDGHSIYGVKLCGREFIRAVIFTCGGSRWRRSLDDSGDLSSDLLSAHDDEASERWALNSVIPGLSYRPRPNSETPSWAAEASEGPVFSRTARSVISEEVLEALRTSDRKGRDVVVGLSNACCKWGCSKSEISSLC

Target ORF information:

RefSeq Version	XM_021475846.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio relaxin 3b (rln3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475846.1

ORF Insert Sequence:

ATGTGTAGAG TTGCAGTACT CGTGTTTGGC CTGCTGGTTG CGCTGCTGGG CGGCGCTCAG 
GCCACAGATG GACACTCGAT CTACGGCGTG AAGCTCTGCG GGAGAGAGTT CATCCGGGCG 
GTGATCTTCA CCTGCGGAGG CTCGCGCTGG AGGAGATCTC TGGATGATTC AGGTGATCTG 
TCCAGTGATC TGCTCTCCGC TCATGATGAC GAGGCCTCTG AAAGATGGGC CTTAAACTCA 
GTCATCCCTG GACTATCATA CAGACCCCGT CCCAACTCGG AGACCCCTAG CTGGGCAGCA 
GAGGCTTCGG AGGGCCCTGT GTTCAGCCGT ACCGCCCGGT CGGTCATCTC AGAGGAAGTG 
CTGGAGGCCC TGCGCACGTC AGACAGGAAA GGCCGTGATG TCGTGGTCGG CCTGTCCAAC 
GCCTGCTGCA AATGGGGCTG CAGCAAGAGC GAGATCAGCT CATTATGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Japanese medaka as a model for studying the relaxin family genes involved in neuroendocrine regulation: Insights from the expression of fish-specific rln3 and insl5 and rxfp3/4-type receptor paralogues.
Molecular and cellular endocrinology4872-11(2019 05)
Alnafea H,Vahkal B,Zelmer CK,Yegorov S,Bogerd J,Good SV

Characterization, cDNA cloning and expression pattern of relaxin gene during embryogenesis of Danio rerio.
Development, growth & differentiation54(5)579-87(2012 Jun)
Fiengo M,Donizetti A,del Gaudio R,Minucci S,Aniello F

New insights into ligand-receptor pairing and coevolution of relaxin family peptides and their receptors in teleosts.
International journal of evolutionary biology2012310278(2012)
Good S,Yegorov S,Martijn J,Franck J,Bogerd J

Relaxin gene family in teleosts: phylogeny, syntenic mapping, selective constraint, and expression analysis.
BMC evolutionary biology9293(2009 Dec)
Good-Avila SV,Yegorov S,Harron S,Bogerd J,Glen P,Ozon J,Wilson BC

Duplicated zebrafish relaxin-3 gene shows a different expression pattern from that of the co-orthologue gene.
Development, growth & differentiation51(8)715-22(2009 Oct)
Donizetti A,Fiengo M,Minucci S,Aniello F