rapgef1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rapgef1a
Entrez Gene ID	558635
Full Name	Rap guanine nucleotide exchange factor (GEF) 1a
Synonyms	si:ch211-127h20.2
General protein information	Preferred Names Rap guanine nucleotide exchange factor (GEF) 1a Names rap guanine nucleotide exchange factor 1 guanine nucleotide-releasing factor 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009303789.2	XP_009302064.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_009303789.3	XP_009302064.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_009303790.3	XP_009302065.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_009303791.2	XP_009302066.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_005166904.4	XP_005166961.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_009303790.2	XP_009302065.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_005166904.3	XP_005166961.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_009303791.3	XP_009302066.1	rap guanine nucleotide exchange factor 1 isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAPGEF1	XP_001167804.1
Mus musculus (house mouse)	Rapgef1	NP_001034175.1
Homo sapiens (human)	RAPGEF1	NP_941372.1
Canis lupus familiaris (dog)	RAPGEF1	XP_005625272.1
Bos taurus (cattle)	RAPGEF1	NP_001192801.1
Rattus norvegicus (Norway rat)	Rapgef1	XP_006233952.1
Gallus gallus (chicken)	RAPGEF1	XP_004945960.1
Xenopus tropicalis (tropical clawed frog)	rapgef1	NP_001039130.1
Macaca mulatta (Rhesus monkey)	RAPGEF1	XP_001105413.1
Danio rerio (zebrafish)	rapgef1a	XP_005166961.1

Gene Ontology

The following rapgef1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa42890	XM_009303791.3 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa14040	XM_005166904.4 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa42888	XM_009303789.3 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa42889	XM_009303790.3 Latest version!	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa42888	XM_009303789.2	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa42889	XM_009303790.2	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa14040	XM_005166904.3	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa42890	XM_009303791.2	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rapgef1a ( XM_009303791.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302066.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa42890
Clone ID Related Accession (Same CDS sequence)	XM_009303791.2 , XM_009303791.3
Accession Version	XM_009303791.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGA CTCTTTTTGC GGTGCTGAAG ATTCGCCCCA GGACTCCGCT 
ATTGAACACA GCAAAGATCC AGCTGACAGA GAGGGCTGGA AGCAGAAGTG TGTGGACTCA 
GATTTGACCT CAGAAGAGGT GGATGAGCTC TGTTTGATTG ACCACGATGA GATCATGTCT 
CGCATAACAC TAAAGCAAGA GAACGACGAC GGCCCTGATG TTAGAGCGGG ATCAGGCGAT 
ATTTTATTAG TTCATGCCAC TGAAACCGAC CGCAAAGATC GGGTTTTATA CTGTGAAGCT 
TTCTTGACCA CGTACAGAAC ATTTCTAACC CCAGAGGACC TCATTAAAAA GCTCCGCTAT 
AGGTACACCA GATTTTCTCT CAGCTCTGAC AGCATCAAAC AACGAGCTAG TAAAAACACC 
TTCTTCGTGC TGGTGCGTGT CGTGGATGAG CTTTGTTTGG TGGAGCTGAC GGAAGACATC 
CTGAAGCAGC TCATGGAGCT TGTGTTCAAT CTCTTGTGTA AAGGGGAGCT TACTTTGGCC 
CGGGTTCTGC GCAAAAACAT CCTGGATAAA GTCCAGCAGA AGAAGTTGCT GCAGTACACT 
AATTCCCTCA AAACACTGGC TGCACGCGGT GTGGCCGCCA GGCCTGGCAC ATTGCATGAC 
TTCCGTAGCC ATGAGATCGC AGACCAGCTG ACGCTCCTCG ATGCAGAGCT CTTTTATAAG 
ATAGAGATCC CGGAGGTTTT ACTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC 
CTGACCCAAT TCACAGAGCA CTTCAACAAC ATGTCGTACT GGGTTCGGTC CATAATAATC 
CTGCAGGAGA AAGCCCAGGA TCGGGAAAAA CTTCTCCTGA AGTTCATCAA AATAATGAAG 
CACTTAAGGA AGCTCAATAA CTTCAACTCA TACTTGGCAA TATTATCAGC GCTGGATTCA 
GCTCCTATCA GAAGACTGGA ATGGCAGAAG CAAACGTCTG AGGGTTTGGA GGAATATTGC 
ACTTTAATTG ACAGTTCGTC CTCTTTCCGG GCGTACAGAG CAGCTTTGGC AGACGTGGAG 
CCTCCATGTA TACCGTACCT AGGTCTCATC CTCCAGGACT TAACGTTTGT GCATCTTGGG 
AATCCTGACC ACATTGAAGG AAAGATCAAC TTTTCCAAAC GCTGGCAGCA GTTTAATATT 
TTAGACACTA TGAGGCGTTT CCAGCAAGTG CACTATGACC TCAAACACAA CGATGACATC 
GTCTCCTTCT TCAACGACTT CAGTGATCAT CTGGCCGAGG AGGCATTGTG GGAGCTTTCC 
TTAAAGATCA AACCCCGCAA CATCACCAGG CGAAAAACAG AGCGAGAGGA AAAGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302066.1
CDS	151..3150
Translation	MSGKIDSKQESQRSHLTSFTMKLKDKFHSPKIKRTPSNKKSKPPPEQPVKSPEKTVTKSLSRLEEHEKEVVSALRYFKTIIDKMAVDKKVLEMLPGSASKVLEAVTGLVQLDSKILSSSRVSSCLNRLYQSLANLIRWSDQVMLEGVNLEDKDTITTVTNVIKAVLDGVKELVKVAIEKQEHPSPTTPVKTIPPVASPQRVPEPPVIETKEPILNDSPAPAENIETPNDVPEEEVAPPKPPLPGLKVPEQHCPPALPPKKRQSAPSPTRIAVVAPMSRTTSGSSLPIGICRQEYEADALQRRFSGGSHSYGGESPRLSPCGSIGILSKSDEQLSSLDQDSGQCSRNTSCETLDTAESYDPDYDFLHQDLSSTEQIPHTHTGGCLSPLPESLHESSSFPVQHQSMPPALPKKERRPPLPPVERLYSQYDNVPDEDMHTPPFPLFGAIPPSNPGVFMGNFGPSENTQIPQSPPPLPEKKSRHILKYMQFMEDYSEPLPSVFYQTPQSESIYEQRNRKRFQEVYGLSDSFSSSDETLPPPALPPKQRQLDSFCGAEDSPQDSAIEHSKDPADREGWKQKCVDSDLTSEEVDELCLIDHDEIMSRITLKQENDDGPDVRAGSGDILLVHATETDRKDRVLYCEAFLTTYRTFLTPEDLIKKLRYRYTRFSLSSDSIKQRASKNTFFVLVRVVDELCLVELTEDILKQLMELVFNLLCKGELTLARVLRKNILDKVQQKKLLQYTNSLKTLAARGVAARPGTLHDFRSHEIADQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIILQEKAQDREKLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLEEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDHIEGKINFSKRWQQFNILDTMRRFQQVHYDLKHNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTEREEKT

Target ORF information:

RefSeq Version	XM_009303791.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303791.2

ORF Insert Sequence:

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGA CTCTTTTTGC GGTGCTGAAG ATTCGCCCCA GGACTCCGCT 
ATTGAACACA GCAAAGATCC AGCTGACAGA GAGGGCTGGA AGCAGAAGTG TGTGGACTCA 
GATTTGACCT CAGAAGAGGT GGATGAGCTC TGTTTGATTG ACCACGATGA GATCATGTCT 
CGCATAACAC TAAAGCAAGA GAACGACGAC GGCCCTGATG TTAGAGCGGG ATCAGGCGAT 
ATTTTATTAG TTCATGCCAC TGAAACCGAC CGCAAAGATC GGGTTTTATA CTGTGAAGCT 
TTCTTGACCA CGTACAGAAC ATTTCTAACC CCAGAGGACC TCATTAAAAA GCTCCGCTAT 
AGGTACACCA GATTTTCTCT CAGCTCTGAC AGCATCAAAC AACGAGCTAG TAAAAACACC 
TTCTTCGTGC TGGTGCGTGT CGTGGATGAG CTTTGTTTGG TGGAGCTGAC GGAAGACATC 
CTGAAGCAGC TCATGGAGCT TGTGTTCAAT CTCTTGTGTA AAGGGGAGCT TACTTTGGCC 
CGGGTTCTGC GCAAAAACAT CCTGGATAAA GTCCAGCAGA AGAAGTTGCT GCAGTACACT 
AATTCCCTCA AAACACTGGC TGCACGCGGT GTGGCCGCCA GGCCTGGCAC ATTGCATGAC 
TTCCGTAGCC ATGAGATCGC AGACCAGCTG ACGCTCCTCG ATGCAGAGCT CTTTTATAAG 
ATAGAGATCC CGGAGGTTTT ACTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC 
CTGACCCAAT TCACAGAGCA CTTCAACAAC ATGTCGTACT GGGTTCGGTC CATAATAATC 
CTGCAGGAGA AAGCCCAGGA TCGGGAAAAA CTTCTCCTGA AGTTCATCAA AATAATGAAG 
CACTTAAGGA AGCTCAATAA CTTCAACTCA TACTTGGCAA TATTATCAGC GCTGGATTCA 
GCTCCTATCA GAAGACTGGA ATGGCAGAAG CAAACGTCTG AGGGTTTGGA GGAATATTGC 
ACTTTAATTG ACAGTTCGTC CTCTTTCCGG GCGTACAGAG CAGCTTTGGC AGACGTGGAG 
CCTCCATGTA TACCGTACCT AGGTCTCATC CTCCAGGACT TAACGTTTGT GCATCTTGGG 
AATCCTGACC ACATTGAAGG AAAGATCAAC TTTTCCAAAC GCTGGCAGCA GTTTAATATT 
TTAGACACTA TGAGGCGTTT CCAGCAAGTG CACTATGACC TCAAACACAA CGATGACATC 
GTCTCCTTCT TCAACGACTT CAGTGATCAT CTGGCCGAGG AGGCATTGTG GGAGCTTTCC 
TTAAAGATCA AACCCCGCAA CATCACCAGG CGAAAAACAG AGCGAGAGGA AAAGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1a ( XM_009303791.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302066.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa42890
Clone ID Related Accession (Same CDS sequence)	XM_009303791.2 , XM_009303791.3
Accession Version	XM_009303791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGA CTCTTTTTGC GGTGCTGAAG ATTCGCCCCA GGACTCCGCT 
ATTGAACACA GCAAAGATCC AGCTGACAGA GAGGGCTGGA AGCAGAAGTG TGTGGACTCA 
GATTTGACCT CAGAAGAGGT GGATGAGCTC TGTTTGATTG ACCACGATGA GATCATGTCT 
CGCATAACAC TAAAGCAAGA GAACGACGAC GGCCCTGATG TTAGAGCGGG ATCAGGCGAT 
ATTTTATTAG TTCATGCCAC TGAAACCGAC CGCAAAGATC GGGTTTTATA CTGTGAAGCT 
TTCTTGACCA CGTACAGAAC ATTTCTAACC CCAGAGGACC TCATTAAAAA GCTCCGCTAT 
AGGTACACCA GATTTTCTCT CAGCTCTGAC AGCATCAAAC AACGAGCTAG TAAAAACACC 
TTCTTCGTGC TGGTGCGTGT CGTGGATGAG CTTTGTTTGG TGGAGCTGAC GGAAGACATC 
CTGAAGCAGC TCATGGAGCT TGTGTTCAAT CTCTTGTGTA AAGGGGAGCT TACTTTGGCC 
CGGGTTCTGC GCAAAAACAT CCTGGATAAA GTCCAGCAGA AGAAGTTGCT GCAGTACACT 
AATTCCCTCA AAACACTGGC TGCACGCGGT GTGGCCGCCA GGCCTGGCAC ATTGCATGAC 
TTCCGTAGCC ATGAGATCGC AGACCAGCTG ACGCTCCTCG ATGCAGAGCT CTTTTATAAG 
ATAGAGATCC CGGAGGTTTT ACTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC 
CTGACCCAAT TCACAGAGCA CTTCAACAAC ATGTCGTACT GGGTTCGGTC CATAATAATC 
CTGCAGGAGA AAGCCCAGGA TCGGGAAAAA CTTCTCCTGA AGTTCATCAA AATAATGAAG 
CACTTAAGGA AGCTCAATAA CTTCAACTCA TACTTGGCAA TATTATCAGC GCTGGATTCA 
GCTCCTATCA GAAGACTGGA ATGGCAGAAG CAAACGTCTG AGGGTTTGGA GGAATATTGC 
ACTTTAATTG ACAGTTCGTC CTCTTTCCGG GCGTACAGAG CAGCTTTGGC AGACGTGGAG 
CCTCCATGTA TACCGTACCT AGGTCTCATC CTCCAGGACT TAACGTTTGT GCATCTTGGG 
AATCCTGACC ACATTGAAGG AAAGATCAAC TTTTCCAAAC GCTGGCAGCA GTTTAATATT 
TTAGACACTA TGAGGCGTTT CCAGCAAGTG CACTATGACC TCAAACACAA CGATGACATC 
GTCTCCTTCT TCAACGACTT CAGTGATCAT CTGGCCGAGG AGGCATTGTG GGAGCTTTCC 
TTAAAGATCA AACCCCGCAA CATCACCAGG CGAAAAACAG AGCGAGAGGA AAAGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302066.1
CDS	157..3156
Translation	MSGKIDSKQESQRSHLTSFTMKLKDKFHSPKIKRTPSNKKSKPPPEQPVKSPEKTVTKSLSRLEEHEKEVVSALRYFKTIIDKMAVDKKVLEMLPGSASKVLEAVTGLVQLDSKILSSSRVSSCLNRLYQSLANLIRWSDQVMLEGVNLEDKDTITTVTNVIKAVLDGVKELVKVAIEKQEHPSPTTPVKTIPPVASPQRVPEPPVIETKEPILNDSPAPAENIETPNDVPEEEVAPPKPPLPGLKVPEQHCPPALPPKKRQSAPSPTRIAVVAPMSRTTSGSSLPIGICRQEYEADALQRRFSGGSHSYGGESPRLSPCGSIGILSKSDEQLSSLDQDSGQCSRNTSCETLDTAESYDPDYDFLHQDLSSTEQIPHTHTGGCLSPLPESLHESSSFPVQHQSMPPALPKKERRPPLPPVERLYSQYDNVPDEDMHTPPFPLFGAIPPSNPGVFMGNFGPSENTQIPQSPPPLPEKKSRHILKYMQFMEDYSEPLPSVFYQTPQSESIYEQRNRKRFQEVYGLSDSFSSSDETLPPPALPPKQRQLDSFCGAEDSPQDSAIEHSKDPADREGWKQKCVDSDLTSEEVDELCLIDHDEIMSRITLKQENDDGPDVRAGSGDILLVHATETDRKDRVLYCEAFLTTYRTFLTPEDLIKKLRYRYTRFSLSSDSIKQRASKNTFFVLVRVVDELCLVELTEDILKQLMELVFNLLCKGELTLARVLRKNILDKVQQKKLLQYTNSLKTLAARGVAARPGTLHDFRSHEIADQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIILQEKAQDREKLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLEEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDHIEGKINFSKRWQQFNILDTMRRFQQVHYDLKHNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTEREEKT

Target ORF information:

RefSeq Version	XM_009303791.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303791.3

ORF Insert Sequence:

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGA CTCTTTTTGC GGTGCTGAAG ATTCGCCCCA GGACTCCGCT 
ATTGAACACA GCAAAGATCC AGCTGACAGA GAGGGCTGGA AGCAGAAGTG TGTGGACTCA 
GATTTGACCT CAGAAGAGGT GGATGAGCTC TGTTTGATTG ACCACGATGA GATCATGTCT 
CGCATAACAC TAAAGCAAGA GAACGACGAC GGCCCTGATG TTAGAGCGGG ATCAGGCGAT 
ATTTTATTAG TTCATGCCAC TGAAACCGAC CGCAAAGATC GGGTTTTATA CTGTGAAGCT 
TTCTTGACCA CGTACAGAAC ATTTCTAACC CCAGAGGACC TCATTAAAAA GCTCCGCTAT 
AGGTACACCA GATTTTCTCT CAGCTCTGAC AGCATCAAAC AACGAGCTAG TAAAAACACC 
TTCTTCGTGC TGGTGCGTGT CGTGGATGAG CTTTGTTTGG TGGAGCTGAC GGAAGACATC 
CTGAAGCAGC TCATGGAGCT TGTGTTCAAT CTCTTGTGTA AAGGGGAGCT TACTTTGGCC 
CGGGTTCTGC GCAAAAACAT CCTGGATAAA GTCCAGCAGA AGAAGTTGCT GCAGTACACT 
AATTCCCTCA AAACACTGGC TGCACGCGGT GTGGCCGCCA GGCCTGGCAC ATTGCATGAC 
TTCCGTAGCC ATGAGATCGC AGACCAGCTG ACGCTCCTCG ATGCAGAGCT CTTTTATAAG 
ATAGAGATCC CGGAGGTTTT ACTTTGGGCA AAAGAACAGA ATGAGGAGAA AAGCCCCAAC 
CTGACCCAAT TCACAGAGCA CTTCAACAAC ATGTCGTACT GGGTTCGGTC CATAATAATC 
CTGCAGGAGA AAGCCCAGGA TCGGGAAAAA CTTCTCCTGA AGTTCATCAA AATAATGAAG 
CACTTAAGGA AGCTCAATAA CTTCAACTCA TACTTGGCAA TATTATCAGC GCTGGATTCA 
GCTCCTATCA GAAGACTGGA ATGGCAGAAG CAAACGTCTG AGGGTTTGGA GGAATATTGC 
ACTTTAATTG ACAGTTCGTC CTCTTTCCGG GCGTACAGAG CAGCTTTGGC AGACGTGGAG 
CCTCCATGTA TACCGTACCT AGGTCTCATC CTCCAGGACT TAACGTTTGT GCATCTTGGG 
AATCCTGACC ACATTGAAGG AAAGATCAAC TTTTCCAAAC GCTGGCAGCA GTTTAATATT 
TTAGACACTA TGAGGCGTTT CCAGCAAGTG CACTATGACC TCAAACACAA CGATGACATC 
GTCTCCTTCT TCAACGACTT CAGTGATCAT CTGGCCGAGG AGGCATTGTG GGAGCTTTCC 
TTAAAGATCA AACCCCGCAA CATCACCAGG CGAAAAACAG AGCGAGAGGA AAAGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1a ( XM_005166904.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166961.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14040
Clone ID Related Accession (Same CDS sequence)	XM_005166904.4 , XM_005166904.3
Accession Version	XM_005166904.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166904.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGC TTCCTCACAC TCTGCGTCTC CTTCCTCCTC CTCTTCCTCG 
TCTCTGTCTT GTCAGCTGCA GCAGTGTGTG TCTGGGCCCG GTCCCGGTGA GACAGAGTCC 
ACTCTCAGCC TTTCTGCATC TAACTCCTCT CTGAACCGCG AGGGCTCTCT GCCTGTCCCT 
ACGAGCGAAA GTGCTAATGA TGAAGGTGGG GAGGGTGAAT ACGTCAATCT GTATTCATCC 
AACCAGGCCA ATGGAGATGT GACTCTCTCC AATGGAGACT CTTTTTGCGG TGCTGAAGAT 
TCGCCCCAGG ACTCCGCTAT TGAACACAGC AAAGATCCAG CTGACAGAGA GGGCTGGAAG 
CAGAAGTGTG TGGACTCAGA TTTGACCTCA GAAGAGGTGG ATGAGCTCTG TTTGATTGAC 
CACGATGAGA TCATGTCTCG CATAACACTA AAGCAAGAGA ACGACGACGG CCCTGATGTT 
AGAGCGGGAT CAGGCGATAT TTTATTAGTT CATGCCACTG AAACCGACCG CAAAGATCGG 
GTTTTATACT GTGAAGCTTT CTTGACCACG TACAGAACAT TTCTAACCCC AGAGGACCTC 
ATTAAAAAGC TCCGCTATAG GTACACCAGA TTTTCTCTCA GCTCTGACAG CATCAAACAA 
CGAGCTAGTA AAAACACCTT CTTCGTGCTG GTGCGTGTCG TGGATGAGCT TTGTTTGGTG 
GAGCTGACGG AAGACATCCT GAAGCAGCTC ATGGAGCTTG TGTTCAATCT CTTGTGTAAA 
GGGGAGCTTA CTTTGGCCCG GGTTCTGCGC AAAAACATCC TGGATAAAGT CCAGCAGAAG 
AAGTTGCTGC AGTACACTAA TTCCCTCAAA ACACTGGCTG CACGCGGTGT GGCCGCCAGG 
CCTGGCACAT TGCATGACTT CCGTAGCCAT GAGATCGCAG ACCAGCTGAC GCTCCTCGAT 
GCAGAGCTCT TTTATAAGAT AGAGATCCCG GAGGTTTTAC TTTGGGCAAA AGAACAGAAT 
GAGGAGAAAA GCCCCAACCT GACCCAATTC ACAGAGCACT TCAACAACAT GTCGTACTGG 
GTTCGGTCCA TAATAATCCT GCAGGAGAAA GCCCAGGATC GGGAAAAACT TCTCCTGAAG 
TTCATCAAAA TAATGAAGCA CTTAAGGAAG CTCAATAACT TCAACTCATA CTTGGCAATA 
TTATCAGCGC TGGATTCAGC TCCTATCAGA AGACTGGAAT GGCAGAAGCA AACGTCTGAG 
GGTTTGGAGG AATATTGCAC TTTAATTGAC AGTTCGTCCT CTTTCCGGGC GTACAGAGCA 
GCTTTGGCAG ACGTGGAGCC TCCATGTATA CCGTACCTAG GTCTCATCCT CCAGGACTTA 
ACGTTTGTGC ATCTTGGGAA TCCTGACCAC ATTGAAGGAA AGATCAACTT TTCCAAACGC 
TGGCAGCAGT TTAATATTTT AGACACTATG AGGCGTTTCC AGCAAGTGCA CTATGACCTC 
AAACACAACG ATGACATCGT CTCCTTCTTC AACGACTTCA GTGATCATCT GGCCGAGGAG 
GCATTGTGGG AGCTTTCCTT AAAGATCAAA CCCCGCAACA TCACCAGGCG AAAAACAGAG 
CGAGAGGAAA AGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166961.1
CDS	158..3415
Translation	MSGKIDSKQESQRSHLTSFTMKLKDKFHSPKIKRTPSNKKSKPPPEQPVKSPEKTVTKSLSRLEEHEKEVVSALRYFKTIIDKMAVDKKVLEMLPGSASKVLEAVTGLVQLDSKILSSSRVSSCLNRLYQSLANLIRWSDQVMLEGVNLEDKDTITTVTNVIKAVLDGVKELVKVAIEKQEHPSPTTPVKTIPPVASPQRVPEPPVIETKEPILNDSPAPAENIETPNDVPEEEVAPPKPPLPGLKVPEQHCPPALPPKKRQSAPSPTRIAVVAPMSRTTSGSSLPIGICRQEYEADALQRRFSGGSHSYGGESPRLSPCGSIGILSKSDEQLSSLDQDSGQCSRNTSCETLDTAESYDPDYDFLHQDLSSTEQIPHTHTGGCLSPLPESLHESSSFPVQHQSMPPALPKKERRPPLPPVERLYSQYDNVPDEDMHTPPFPLFGAIPPSNPGVFMGNFGPSENTQIPQSPPPLPEKKSRHILKYMQFMEDYSEPLPSVFYQTPQSESIYEQRNRKRFQEVYGLSDSFSSSDETLPPPALPPKQRQLASSHSASPSSSSSSSLSCQLQQCVSGPGPGETESTLSLSASNSSLNREGSLPVPTSESANDEGGEGEYVNLYSSNQANGDVTLSNGDSFCGAEDSPQDSAIEHSKDPADREGWKQKCVDSDLTSEEVDELCLIDHDEIMSRITLKQENDDGPDVRAGSGDILLVHATETDRKDRVLYCEAFLTTYRTFLTPEDLIKKLRYRYTRFSLSSDSIKQRASKNTFFVLVRVVDELCLVELTEDILKQLMELVFNLLCKGELTLARVLRKNILDKVQQKKLLQYTNSLKTLAARGVAARPGTLHDFRSHEIADQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIILQEKAQDREKLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLEEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDHIEGKINFSKRWQQFNILDTMRRFQQVHYDLKHNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTEREEKT

Target ORF information:

RefSeq Version	XM_005166904.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166904.4

ORF Insert Sequence:

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGC TTCCTCACAC TCTGCGTCTC CTTCCTCCTC CTCTTCCTCG 
TCTCTGTCTT GTCAGCTGCA GCAGTGTGTG TCTGGGCCCG GTCCCGGTGA GACAGAGTCC 
ACTCTCAGCC TTTCTGCATC TAACTCCTCT CTGAACCGCG AGGGCTCTCT GCCTGTCCCT 
ACGAGCGAAA GTGCTAATGA TGAAGGTGGG GAGGGTGAAT ACGTCAATCT GTATTCATCC 
AACCAGGCCA ATGGAGATGT GACTCTCTCC AATGGAGACT CTTTTTGCGG TGCTGAAGAT 
TCGCCCCAGG ACTCCGCTAT TGAACACAGC AAAGATCCAG CTGACAGAGA GGGCTGGAAG 
CAGAAGTGTG TGGACTCAGA TTTGACCTCA GAAGAGGTGG ATGAGCTCTG TTTGATTGAC 
CACGATGAGA TCATGTCTCG CATAACACTA AAGCAAGAGA ACGACGACGG CCCTGATGTT 
AGAGCGGGAT CAGGCGATAT TTTATTAGTT CATGCCACTG AAACCGACCG CAAAGATCGG 
GTTTTATACT GTGAAGCTTT CTTGACCACG TACAGAACAT TTCTAACCCC AGAGGACCTC 
ATTAAAAAGC TCCGCTATAG GTACACCAGA TTTTCTCTCA GCTCTGACAG CATCAAACAA 
CGAGCTAGTA AAAACACCTT CTTCGTGCTG GTGCGTGTCG TGGATGAGCT TTGTTTGGTG 
GAGCTGACGG AAGACATCCT GAAGCAGCTC ATGGAGCTTG TGTTCAATCT CTTGTGTAAA 
GGGGAGCTTA CTTTGGCCCG GGTTCTGCGC AAAAACATCC TGGATAAAGT CCAGCAGAAG 
AAGTTGCTGC AGTACACTAA TTCCCTCAAA ACACTGGCTG CACGCGGTGT GGCCGCCAGG 
CCTGGCACAT TGCATGACTT CCGTAGCCAT GAGATCGCAG ACCAGCTGAC GCTCCTCGAT 
GCAGAGCTCT TTTATAAGAT AGAGATCCCG GAGGTTTTAC TTTGGGCAAA AGAACAGAAT 
GAGGAGAAAA GCCCCAACCT GACCCAATTC ACAGAGCACT TCAACAACAT GTCGTACTGG 
GTTCGGTCCA TAATAATCCT GCAGGAGAAA GCCCAGGATC GGGAAAAACT TCTCCTGAAG 
TTCATCAAAA TAATGAAGCA CTTAAGGAAG CTCAATAACT TCAACTCATA CTTGGCAATA 
TTATCAGCGC TGGATTCAGC TCCTATCAGA AGACTGGAAT GGCAGAAGCA AACGTCTGAG 
GGTTTGGAGG AATATTGCAC TTTAATTGAC AGTTCGTCCT CTTTCCGGGC GTACAGAGCA 
GCTTTGGCAG ACGTGGAGCC TCCATGTATA CCGTACCTAG GTCTCATCCT CCAGGACTTA 
ACGTTTGTGC ATCTTGGGAA TCCTGACCAC ATTGAAGGAA AGATCAACTT TTCCAAACGC 
TGGCAGCAGT TTAATATTTT AGACACTATG AGGCGTTTCC AGCAAGTGCA CTATGACCTC 
AAACACAACG ATGACATCGT CTCCTTCTTC AACGACTTCA GTGATCATCT GGCCGAGGAG 
GCATTGTGGG AGCTTTCCTT AAAGATCAAA CCCCGCAACA TCACCAGGCG AAAAACAGAG 
CGAGAGGAAA AGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1a ( XM_005166904.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166961.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14040
Clone ID Related Accession (Same CDS sequence)	XM_005166904.4 , XM_005166904.3
Accession Version	XM_005166904.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGC TTCCTCACAC TCTGCGTCTC CTTCCTCCTC CTCTTCCTCG 
TCTCTGTCTT GTCAGCTGCA GCAGTGTGTG TCTGGGCCCG GTCCCGGTGA GACAGAGTCC 
ACTCTCAGCC TTTCTGCATC TAACTCCTCT CTGAACCGCG AGGGCTCTCT GCCTGTCCCT 
ACGAGCGAAA GTGCTAATGA TGAAGGTGGG GAGGGTGAAT ACGTCAATCT GTATTCATCC 
AACCAGGCCA ATGGAGATGT GACTCTCTCC AATGGAGACT CTTTTTGCGG TGCTGAAGAT 
TCGCCCCAGG ACTCCGCTAT TGAACACAGC AAAGATCCAG CTGACAGAGA GGGCTGGAAG 
CAGAAGTGTG TGGACTCAGA TTTGACCTCA GAAGAGGTGG ATGAGCTCTG TTTGATTGAC 
CACGATGAGA TCATGTCTCG CATAACACTA AAGCAAGAGA ACGACGACGG CCCTGATGTT 
AGAGCGGGAT CAGGCGATAT TTTATTAGTT CATGCCACTG AAACCGACCG CAAAGATCGG 
GTTTTATACT GTGAAGCTTT CTTGACCACG TACAGAACAT TTCTAACCCC AGAGGACCTC 
ATTAAAAAGC TCCGCTATAG GTACACCAGA TTTTCTCTCA GCTCTGACAG CATCAAACAA 
CGAGCTAGTA AAAACACCTT CTTCGTGCTG GTGCGTGTCG TGGATGAGCT TTGTTTGGTG 
GAGCTGACGG AAGACATCCT GAAGCAGCTC ATGGAGCTTG TGTTCAATCT CTTGTGTAAA 
GGGGAGCTTA CTTTGGCCCG GGTTCTGCGC AAAAACATCC TGGATAAAGT CCAGCAGAAG 
AAGTTGCTGC AGTACACTAA TTCCCTCAAA ACACTGGCTG CACGCGGTGT GGCCGCCAGG 
CCTGGCACAT TGCATGACTT CCGTAGCCAT GAGATCGCAG ACCAGCTGAC GCTCCTCGAT 
GCAGAGCTCT TTTATAAGAT AGAGATCCCG GAGGTTTTAC TTTGGGCAAA AGAACAGAAT 
GAGGAGAAAA GCCCCAACCT GACCCAATTC ACAGAGCACT TCAACAACAT GTCGTACTGG 
GTTCGGTCCA TAATAATCCT GCAGGAGAAA GCCCAGGATC GGGAAAAACT TCTCCTGAAG 
TTCATCAAAA TAATGAAGCA CTTAAGGAAG CTCAATAACT TCAACTCATA CTTGGCAATA 
TTATCAGCGC TGGATTCAGC TCCTATCAGA AGACTGGAAT GGCAGAAGCA AACGTCTGAG 
GGTTTGGAGG AATATTGCAC TTTAATTGAC AGTTCGTCCT CTTTCCGGGC GTACAGAGCA 
GCTTTGGCAG ACGTGGAGCC TCCATGTATA CCGTACCTAG GTCTCATCCT CCAGGACTTA 
ACGTTTGTGC ATCTTGGGAA TCCTGACCAC ATTGAAGGAA AGATCAACTT TTCCAAACGC 
TGGCAGCAGT TTAATATTTT AGACACTATG AGGCGTTTCC AGCAAGTGCA CTATGACCTC 
AAACACAACG ATGACATCGT CTCCTTCTTC AACGACTTCA GTGATCATCT GGCCGAGGAG 
GCATTGTGGG AGCTTTCCTT AAAGATCAAA CCCCGCAACA TCACCAGGCG AAAAACAGAG 
CGAGAGGAAA AGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166961.1
CDS	151..3408
Translation	MSGKIDSKQESQRSHLTSFTMKLKDKFHSPKIKRTPSNKKSKPPPEQPVKSPEKTVTKSLSRLEEHEKEVVSALRYFKTIIDKMAVDKKVLEMLPGSASKVLEAVTGLVQLDSKILSSSRVSSCLNRLYQSLANLIRWSDQVMLEGVNLEDKDTITTVTNVIKAVLDGVKELVKVAIEKQEHPSPTTPVKTIPPVASPQRVPEPPVIETKEPILNDSPAPAENIETPNDVPEEEVAPPKPPLPGLKVPEQHCPPALPPKKRQSAPSPTRIAVVAPMSRTTSGSSLPIGICRQEYEADALQRRFSGGSHSYGGESPRLSPCGSIGILSKSDEQLSSLDQDSGQCSRNTSCETLDTAESYDPDYDFLHQDLSSTEQIPHTHTGGCLSPLPESLHESSSFPVQHQSMPPALPKKERRPPLPPVERLYSQYDNVPDEDMHTPPFPLFGAIPPSNPGVFMGNFGPSENTQIPQSPPPLPEKKSRHILKYMQFMEDYSEPLPSVFYQTPQSESIYEQRNRKRFQEVYGLSDSFSSSDETLPPPALPPKQRQLASSHSASPSSSSSSSLSCQLQQCVSGPGPGETESTLSLSASNSSLNREGSLPVPTSESANDEGGEGEYVNLYSSNQANGDVTLSNGDSFCGAEDSPQDSAIEHSKDPADREGWKQKCVDSDLTSEEVDELCLIDHDEIMSRITLKQENDDGPDVRAGSGDILLVHATETDRKDRVLYCEAFLTTYRTFLTPEDLIKKLRYRYTRFSLSSDSIKQRASKNTFFVLVRVVDELCLVELTEDILKQLMELVFNLLCKGELTLARVLRKNILDKVQQKKLLQYTNSLKTLAARGVAARPGTLHDFRSHEIADQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIILQEKAQDREKLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLEEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDHIEGKINFSKRWQQFNILDTMRRFQQVHYDLKHNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTEREEKT

Target ORF information:

RefSeq Version	XM_005166904.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166904.3

ORF Insert Sequence:

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGC TTCCTCACAC TCTGCGTCTC CTTCCTCCTC CTCTTCCTCG 
TCTCTGTCTT GTCAGCTGCA GCAGTGTGTG TCTGGGCCCG GTCCCGGTGA GACAGAGTCC 
ACTCTCAGCC TTTCTGCATC TAACTCCTCT CTGAACCGCG AGGGCTCTCT GCCTGTCCCT 
ACGAGCGAAA GTGCTAATGA TGAAGGTGGG GAGGGTGAAT ACGTCAATCT GTATTCATCC 
AACCAGGCCA ATGGAGATGT GACTCTCTCC AATGGAGACT CTTTTTGCGG TGCTGAAGAT 
TCGCCCCAGG ACTCCGCTAT TGAACACAGC AAAGATCCAG CTGACAGAGA GGGCTGGAAG 
CAGAAGTGTG TGGACTCAGA TTTGACCTCA GAAGAGGTGG ATGAGCTCTG TTTGATTGAC 
CACGATGAGA TCATGTCTCG CATAACACTA AAGCAAGAGA ACGACGACGG CCCTGATGTT 
AGAGCGGGAT CAGGCGATAT TTTATTAGTT CATGCCACTG AAACCGACCG CAAAGATCGG 
GTTTTATACT GTGAAGCTTT CTTGACCACG TACAGAACAT TTCTAACCCC AGAGGACCTC 
ATTAAAAAGC TCCGCTATAG GTACACCAGA TTTTCTCTCA GCTCTGACAG CATCAAACAA 
CGAGCTAGTA AAAACACCTT CTTCGTGCTG GTGCGTGTCG TGGATGAGCT TTGTTTGGTG 
GAGCTGACGG AAGACATCCT GAAGCAGCTC ATGGAGCTTG TGTTCAATCT CTTGTGTAAA 
GGGGAGCTTA CTTTGGCCCG GGTTCTGCGC AAAAACATCC TGGATAAAGT CCAGCAGAAG 
AAGTTGCTGC AGTACACTAA TTCCCTCAAA ACACTGGCTG CACGCGGTGT GGCCGCCAGG 
CCTGGCACAT TGCATGACTT CCGTAGCCAT GAGATCGCAG ACCAGCTGAC GCTCCTCGAT 
GCAGAGCTCT TTTATAAGAT AGAGATCCCG GAGGTTTTAC TTTGGGCAAA AGAACAGAAT 
GAGGAGAAAA GCCCCAACCT GACCCAATTC ACAGAGCACT TCAACAACAT GTCGTACTGG 
GTTCGGTCCA TAATAATCCT GCAGGAGAAA GCCCAGGATC GGGAAAAACT TCTCCTGAAG 
TTCATCAAAA TAATGAAGCA CTTAAGGAAG CTCAATAACT TCAACTCATA CTTGGCAATA 
TTATCAGCGC TGGATTCAGC TCCTATCAGA AGACTGGAAT GGCAGAAGCA AACGTCTGAG 
GGTTTGGAGG AATATTGCAC TTTAATTGAC AGTTCGTCCT CTTTCCGGGC GTACAGAGCA 
GCTTTGGCAG ACGTGGAGCC TCCATGTATA CCGTACCTAG GTCTCATCCT CCAGGACTTA 
ACGTTTGTGC ATCTTGGGAA TCCTGACCAC ATTGAAGGAA AGATCAACTT TTCCAAACGC 
TGGCAGCAGT TTAATATTTT AGACACTATG AGGCGTTTCC AGCAAGTGCA CTATGACCTC 
AAACACAACG ATGACATCGT CTCCTTCTTC AACGACTTCA GTGATCATCT GGCCGAGGAG 
GCATTGTGGG AGCTTTCCTT AAAGATCAAA CCCCGCAACA TCACCAGGCG AAAAACAGAG 
CGAGAGGAAA AGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1a ( XM_009303789.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302064.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X2, mRNA.

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CloneID	ODa42888
Clone ID Related Accession (Same CDS sequence)	XM_009303789.2 , XM_009303789.3
Accession Version	XM_009303789.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303789.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGC TTCCTCACAC TCTGCGTCTC CTTCCTCCTC CTCTTCCTCG 
TCTCTGTCTT GTCAGCTGCA GCAGTGTGTG TCTGGGCCCG GTCCCGGTGA GACAGAGTCC 
ACTCTCAGCC TTTCTGCATC TAACTCCTCT CTGAACCGCG AGGGCTCTCT GCCTGTCCCT 
ACGGACTCTT TTTGCGGTGC TGAAGATTCG CCCCAGGACT CCGCTATTGA ACACAGCAAA 
GATCCAGCTG ACAGAGAGGG CTGGAAGCAG AAGTGTGTGG ACTCAGATTT GACCTCAGAA 
GAGGTGGATG AGCTCTGTTT GATTGACCAC GATGAGATCA TGTCTCGCAT AACACTAAAG 
CAAGAGAACG ACGACGGCCC TGATGTTAGA GCGGGATCAG GCGATATTTT ATTAGTTCAT 
GCCACTGAAA CCGACCGCAA AGATCGGGTT TTATACTGTG AAGCTTTCTT GACCACGTAC 
AGAACATTTC TAACCCCAGA GGACCTCATT AAAAAGCTCC GCTATAGGTA CACCAGATTT 
TCTCTCAGCT CTGACAGCAT CAAACAACGA GCTAGTAAAA ACACCTTCTT CGTGCTGGTG 
CGTGTCGTGG ATGAGCTTTG TTTGGTGGAG CTGACGGAAG ACATCCTGAA GCAGCTCATG 
GAGCTTGTGT TCAATCTCTT GTGTAAAGGG GAGCTTACTT TGGCCCGGGT TCTGCGCAAA 
AACATCCTGG ATAAAGTCCA GCAGAAGAAG TTGCTGCAGT ACACTAATTC CCTCAAAACA 
CTGGCTGCAC GCGGTGTGGC CGCCAGGCCT GGCACATTGC ATGACTTCCG TAGCCATGAG 
ATCGCAGACC AGCTGACGCT CCTCGATGCA GAGCTCTTTT ATAAGATAGA GATCCCGGAG 
GTTTTACTTT GGGCAAAAGA ACAGAATGAG GAGAAAAGCC CCAACCTGAC CCAATTCACA 
GAGCACTTCA ACAACATGTC GTACTGGGTT CGGTCCATAA TAATCCTGCA GGAGAAAGCC 
CAGGATCGGG AAAAACTTCT CCTGAAGTTC ATCAAAATAA TGAAGCACTT AAGGAAGCTC 
AATAACTTCA ACTCATACTT GGCAATATTA TCAGCGCTGG ATTCAGCTCC TATCAGAAGA 
CTGGAATGGC AGAAGCAAAC GTCTGAGGGT TTGGAGGAAT ATTGCACTTT AATTGACAGT 
TCGTCCTCTT TCCGGGCGTA CAGAGCAGCT TTGGCAGACG TGGAGCCTCC ATGTATACCG 
TACCTAGGTC TCATCCTCCA GGACTTAACG TTTGTGCATC TTGGGAATCC TGACCACATT 
GAAGGAAAGA TCAACTTTTC CAAACGCTGG CAGCAGTTTA ATATTTTAGA CACTATGAGG 
CGTTTCCAGC AAGTGCACTA TGACCTCAAA CACAACGATG ACATCGTCTC CTTCTTCAAC 
GACTTCAGTG ATCATCTGGC CGAGGAGGCA TTGTGGGAGC TTTCCTTAAA GATCAAACCC 
CGCAACATCA CCAGGCGAAA AACAGAGCGA GAGGAAAAGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302064.1
CDS	151..3315
Translation	MSGKIDSKQESQRSHLTSFTMKLKDKFHSPKIKRTPSNKKSKPPPEQPVKSPEKTVTKSLSRLEEHEKEVVSALRYFKTIIDKMAVDKKVLEMLPGSASKVLEAVTGLVQLDSKILSSSRVSSCLNRLYQSLANLIRWSDQVMLEGVNLEDKDTITTVTNVIKAVLDGVKELVKVAIEKQEHPSPTTPVKTIPPVASPQRVPEPPVIETKEPILNDSPAPAENIETPNDVPEEEVAPPKPPLPGLKVPEQHCPPALPPKKRQSAPSPTRIAVVAPMSRTTSGSSLPIGICRQEYEADALQRRFSGGSHSYGGESPRLSPCGSIGILSKSDEQLSSLDQDSGQCSRNTSCETLDTAESYDPDYDFLHQDLSSTEQIPHTHTGGCLSPLPESLHESSSFPVQHQSMPPALPKKERRPPLPPVERLYSQYDNVPDEDMHTPPFPLFGAIPPSNPGVFMGNFGPSENTQIPQSPPPLPEKKSRHILKYMQFMEDYSEPLPSVFYQTPQSESIYEQRNRKRFQEVYGLSDSFSSSDETLPPPALPPKQRQLASSHSASPSSSSSSSLSCQLQQCVSGPGPGETESTLSLSASNSSLNREGSLPVPTDSFCGAEDSPQDSAIEHSKDPADREGWKQKCVDSDLTSEEVDELCLIDHDEIMSRITLKQENDDGPDVRAGSGDILLVHATETDRKDRVLYCEAFLTTYRTFLTPEDLIKKLRYRYTRFSLSSDSIKQRASKNTFFVLVRVVDELCLVELTEDILKQLMELVFNLLCKGELTLARVLRKNILDKVQQKKLLQYTNSLKTLAARGVAARPGTLHDFRSHEIADQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIILQEKAQDREKLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLEEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDHIEGKINFSKRWQQFNILDTMRRFQQVHYDLKHNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTEREEKT

Target ORF information:

RefSeq Version	XM_009303789.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303789.2

ORF Insert Sequence:

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGC TTCCTCACAC TCTGCGTCTC CTTCCTCCTC CTCTTCCTCG 
TCTCTGTCTT GTCAGCTGCA GCAGTGTGTG TCTGGGCCCG GTCCCGGTGA GACAGAGTCC 
ACTCTCAGCC TTTCTGCATC TAACTCCTCT CTGAACCGCG AGGGCTCTCT GCCTGTCCCT 
ACGGACTCTT TTTGCGGTGC TGAAGATTCG CCCCAGGACT CCGCTATTGA ACACAGCAAA 
GATCCAGCTG ACAGAGAGGG CTGGAAGCAG AAGTGTGTGG ACTCAGATTT GACCTCAGAA 
GAGGTGGATG AGCTCTGTTT GATTGACCAC GATGAGATCA TGTCTCGCAT AACACTAAAG 
CAAGAGAACG ACGACGGCCC TGATGTTAGA GCGGGATCAG GCGATATTTT ATTAGTTCAT 
GCCACTGAAA CCGACCGCAA AGATCGGGTT TTATACTGTG AAGCTTTCTT GACCACGTAC 
AGAACATTTC TAACCCCAGA GGACCTCATT AAAAAGCTCC GCTATAGGTA CACCAGATTT 
TCTCTCAGCT CTGACAGCAT CAAACAACGA GCTAGTAAAA ACACCTTCTT CGTGCTGGTG 
CGTGTCGTGG ATGAGCTTTG TTTGGTGGAG CTGACGGAAG ACATCCTGAA GCAGCTCATG 
GAGCTTGTGT TCAATCTCTT GTGTAAAGGG GAGCTTACTT TGGCCCGGGT TCTGCGCAAA 
AACATCCTGG ATAAAGTCCA GCAGAAGAAG TTGCTGCAGT ACACTAATTC CCTCAAAACA 
CTGGCTGCAC GCGGTGTGGC CGCCAGGCCT GGCACATTGC ATGACTTCCG TAGCCATGAG 
ATCGCAGACC AGCTGACGCT CCTCGATGCA GAGCTCTTTT ATAAGATAGA GATCCCGGAG 
GTTTTACTTT GGGCAAAAGA ACAGAATGAG GAGAAAAGCC CCAACCTGAC CCAATTCACA 
GAGCACTTCA ACAACATGTC GTACTGGGTT CGGTCCATAA TAATCCTGCA GGAGAAAGCC 
CAGGATCGGG AAAAACTTCT CCTGAAGTTC ATCAAAATAA TGAAGCACTT AAGGAAGCTC 
AATAACTTCA ACTCATACTT GGCAATATTA TCAGCGCTGG ATTCAGCTCC TATCAGAAGA 
CTGGAATGGC AGAAGCAAAC GTCTGAGGGT TTGGAGGAAT ATTGCACTTT AATTGACAGT 
TCGTCCTCTT TCCGGGCGTA CAGAGCAGCT TTGGCAGACG TGGAGCCTCC ATGTATACCG 
TACCTAGGTC TCATCCTCCA GGACTTAACG TTTGTGCATC TTGGGAATCC TGACCACATT 
GAAGGAAAGA TCAACTTTTC CAAACGCTGG CAGCAGTTTA ATATTTTAGA CACTATGAGG 
CGTTTCCAGC AAGTGCACTA TGACCTCAAA CACAACGATG ACATCGTCTC CTTCTTCAAC 
GACTTCAGTG ATCATCTGGC CGAGGAGGCA TTGTGGGAGC TTTCCTTAAA GATCAAACCC 
CGCAACATCA CCAGGCGAAA AACAGAGCGA GAGGAAAAGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1a ( XM_009303789.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302064.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42888
Clone ID Related Accession (Same CDS sequence)	XM_009303789.2 , XM_009303789.3
Accession Version	XM_009303789.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303789.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGC TTCCTCACAC TCTGCGTCTC CTTCCTCCTC CTCTTCCTCG 
TCTCTGTCTT GTCAGCTGCA GCAGTGTGTG TCTGGGCCCG GTCCCGGTGA GACAGAGTCC 
ACTCTCAGCC TTTCTGCATC TAACTCCTCT CTGAACCGCG AGGGCTCTCT GCCTGTCCCT 
ACGGACTCTT TTTGCGGTGC TGAAGATTCG CCCCAGGACT CCGCTATTGA ACACAGCAAA 
GATCCAGCTG ACAGAGAGGG CTGGAAGCAG AAGTGTGTGG ACTCAGATTT GACCTCAGAA 
GAGGTGGATG AGCTCTGTTT GATTGACCAC GATGAGATCA TGTCTCGCAT AACACTAAAG 
CAAGAGAACG ACGACGGCCC TGATGTTAGA GCGGGATCAG GCGATATTTT ATTAGTTCAT 
GCCACTGAAA CCGACCGCAA AGATCGGGTT TTATACTGTG AAGCTTTCTT GACCACGTAC 
AGAACATTTC TAACCCCAGA GGACCTCATT AAAAAGCTCC GCTATAGGTA CACCAGATTT 
TCTCTCAGCT CTGACAGCAT CAAACAACGA GCTAGTAAAA ACACCTTCTT CGTGCTGGTG 
CGTGTCGTGG ATGAGCTTTG TTTGGTGGAG CTGACGGAAG ACATCCTGAA GCAGCTCATG 
GAGCTTGTGT TCAATCTCTT GTGTAAAGGG GAGCTTACTT TGGCCCGGGT TCTGCGCAAA 
AACATCCTGG ATAAAGTCCA GCAGAAGAAG TTGCTGCAGT ACACTAATTC CCTCAAAACA 
CTGGCTGCAC GCGGTGTGGC CGCCAGGCCT GGCACATTGC ATGACTTCCG TAGCCATGAG 
ATCGCAGACC AGCTGACGCT CCTCGATGCA GAGCTCTTTT ATAAGATAGA GATCCCGGAG 
GTTTTACTTT GGGCAAAAGA ACAGAATGAG GAGAAAAGCC CCAACCTGAC CCAATTCACA 
GAGCACTTCA ACAACATGTC GTACTGGGTT CGGTCCATAA TAATCCTGCA GGAGAAAGCC 
CAGGATCGGG AAAAACTTCT CCTGAAGTTC ATCAAAATAA TGAAGCACTT AAGGAAGCTC 
AATAACTTCA ACTCATACTT GGCAATATTA TCAGCGCTGG ATTCAGCTCC TATCAGAAGA 
CTGGAATGGC AGAAGCAAAC GTCTGAGGGT TTGGAGGAAT ATTGCACTTT AATTGACAGT 
TCGTCCTCTT TCCGGGCGTA CAGAGCAGCT TTGGCAGACG TGGAGCCTCC ATGTATACCG 
TACCTAGGTC TCATCCTCCA GGACTTAACG TTTGTGCATC TTGGGAATCC TGACCACATT 
GAAGGAAAGA TCAACTTTTC CAAACGCTGG CAGCAGTTTA ATATTTTAGA CACTATGAGG 
CGTTTCCAGC AAGTGCACTA TGACCTCAAA CACAACGATG ACATCGTCTC CTTCTTCAAC 
GACTTCAGTG ATCATCTGGC CGAGGAGGCA TTGTGGGAGC TTTCCTTAAA GATCAAACCC 
CGCAACATCA CCAGGCGAAA AACAGAGCGA GAGGAAAAGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302064.1
CDS	158..3322
Translation	MSGKIDSKQESQRSHLTSFTMKLKDKFHSPKIKRTPSNKKSKPPPEQPVKSPEKTVTKSLSRLEEHEKEVVSALRYFKTIIDKMAVDKKVLEMLPGSASKVLEAVTGLVQLDSKILSSSRVSSCLNRLYQSLANLIRWSDQVMLEGVNLEDKDTITTVTNVIKAVLDGVKELVKVAIEKQEHPSPTTPVKTIPPVASPQRVPEPPVIETKEPILNDSPAPAENIETPNDVPEEEVAPPKPPLPGLKVPEQHCPPALPPKKRQSAPSPTRIAVVAPMSRTTSGSSLPIGICRQEYEADALQRRFSGGSHSYGGESPRLSPCGSIGILSKSDEQLSSLDQDSGQCSRNTSCETLDTAESYDPDYDFLHQDLSSTEQIPHTHTGGCLSPLPESLHESSSFPVQHQSMPPALPKKERRPPLPPVERLYSQYDNVPDEDMHTPPFPLFGAIPPSNPGVFMGNFGPSENTQIPQSPPPLPEKKSRHILKYMQFMEDYSEPLPSVFYQTPQSESIYEQRNRKRFQEVYGLSDSFSSSDETLPPPALPPKQRQLASSHSASPSSSSSSSLSCQLQQCVSGPGPGETESTLSLSASNSSLNREGSLPVPTDSFCGAEDSPQDSAIEHSKDPADREGWKQKCVDSDLTSEEVDELCLIDHDEIMSRITLKQENDDGPDVRAGSGDILLVHATETDRKDRVLYCEAFLTTYRTFLTPEDLIKKLRYRYTRFSLSSDSIKQRASKNTFFVLVRVVDELCLVELTEDILKQLMELVFNLLCKGELTLARVLRKNILDKVQQKKLLQYTNSLKTLAARGVAARPGTLHDFRSHEIADQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIILQEKAQDREKLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLEEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDHIEGKINFSKRWQQFNILDTMRRFQQVHYDLKHNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTEREEKT

Target ORF information:

RefSeq Version	XM_009303789.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303789.3

ORF Insert Sequence:

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGGC TTCCTCACAC TCTGCGTCTC CTTCCTCCTC CTCTTCCTCG 
TCTCTGTCTT GTCAGCTGCA GCAGTGTGTG TCTGGGCCCG GTCCCGGTGA GACAGAGTCC 
ACTCTCAGCC TTTCTGCATC TAACTCCTCT CTGAACCGCG AGGGCTCTCT GCCTGTCCCT 
ACGGACTCTT TTTGCGGTGC TGAAGATTCG CCCCAGGACT CCGCTATTGA ACACAGCAAA 
GATCCAGCTG ACAGAGAGGG CTGGAAGCAG AAGTGTGTGG ACTCAGATTT GACCTCAGAA 
GAGGTGGATG AGCTCTGTTT GATTGACCAC GATGAGATCA TGTCTCGCAT AACACTAAAG 
CAAGAGAACG ACGACGGCCC TGATGTTAGA GCGGGATCAG GCGATATTTT ATTAGTTCAT 
GCCACTGAAA CCGACCGCAA AGATCGGGTT TTATACTGTG AAGCTTTCTT GACCACGTAC 
AGAACATTTC TAACCCCAGA GGACCTCATT AAAAAGCTCC GCTATAGGTA CACCAGATTT 
TCTCTCAGCT CTGACAGCAT CAAACAACGA GCTAGTAAAA ACACCTTCTT CGTGCTGGTG 
CGTGTCGTGG ATGAGCTTTG TTTGGTGGAG CTGACGGAAG ACATCCTGAA GCAGCTCATG 
GAGCTTGTGT TCAATCTCTT GTGTAAAGGG GAGCTTACTT TGGCCCGGGT TCTGCGCAAA 
AACATCCTGG ATAAAGTCCA GCAGAAGAAG TTGCTGCAGT ACACTAATTC CCTCAAAACA 
CTGGCTGCAC GCGGTGTGGC CGCCAGGCCT GGCACATTGC ATGACTTCCG TAGCCATGAG 
ATCGCAGACC AGCTGACGCT CCTCGATGCA GAGCTCTTTT ATAAGATAGA GATCCCGGAG 
GTTTTACTTT GGGCAAAAGA ACAGAATGAG GAGAAAAGCC CCAACCTGAC CCAATTCACA 
GAGCACTTCA ACAACATGTC GTACTGGGTT CGGTCCATAA TAATCCTGCA GGAGAAAGCC 
CAGGATCGGG AAAAACTTCT CCTGAAGTTC ATCAAAATAA TGAAGCACTT AAGGAAGCTC 
AATAACTTCA ACTCATACTT GGCAATATTA TCAGCGCTGG ATTCAGCTCC TATCAGAAGA 
CTGGAATGGC AGAAGCAAAC GTCTGAGGGT TTGGAGGAAT ATTGCACTTT AATTGACAGT 
TCGTCCTCTT TCCGGGCGTA CAGAGCAGCT TTGGCAGACG TGGAGCCTCC ATGTATACCG 
TACCTAGGTC TCATCCTCCA GGACTTAACG TTTGTGCATC TTGGGAATCC TGACCACATT 
GAAGGAAAGA TCAACTTTTC CAAACGCTGG CAGCAGTTTA ATATTTTAGA CACTATGAGG 
CGTTTCCAGC AAGTGCACTA TGACCTCAAA CACAACGATG ACATCGTCTC CTTCTTCAAC 
GACTTCAGTG ATCATCTGGC CGAGGAGGCA TTGTGGGAGC TTTCCTTAAA GATCAAACCC 
CGCAACATCA CCAGGCGAAA AACAGAGCGA GAGGAAAAGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1a ( XM_009303790.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302065.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42889
Clone ID Related Accession (Same CDS sequence)	XM_009303790.3 , XM_009303790.2
Accession Version	XM_009303790.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303790.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGAG CGAAAGTGCT AATGATGAAG GTGGGGAGGG TGAATACGTC 
AATCTGTATT CATCCAACCA GGCCAATGGA GATGTGACTC TCTCCAATGG AGACTCTTTT 
TGCGGTGCTG AAGATTCGCC CCAGGACTCC GCTATTGAAC ACAGCAAAGA TCCAGCTGAC 
AGAGAGGGCT GGAAGCAGAA GTGTGTGGAC TCAGATTTGA CCTCAGAAGA GGTGGATGAG 
CTCTGTTTGA TTGACCACGA TGAGATCATG TCTCGCATAA CACTAAAGCA AGAGAACGAC 
GACGGCCCTG ATGTTAGAGC GGGATCAGGC GATATTTTAT TAGTTCATGC CACTGAAACC 
GACCGCAAAG ATCGGGTTTT ATACTGTGAA GCTTTCTTGA CCACGTACAG AACATTTCTA 
ACCCCAGAGG ACCTCATTAA AAAGCTCCGC TATAGGTACA CCAGATTTTC TCTCAGCTCT 
GACAGCATCA AACAACGAGC TAGTAAAAAC ACCTTCTTCG TGCTGGTGCG TGTCGTGGAT 
GAGCTTTGTT TGGTGGAGCT GACGGAAGAC ATCCTGAAGC AGCTCATGGA GCTTGTGTTC 
AATCTCTTGT GTAAAGGGGA GCTTACTTTG GCCCGGGTTC TGCGCAAAAA CATCCTGGAT 
AAAGTCCAGC AGAAGAAGTT GCTGCAGTAC ACTAATTCCC TCAAAACACT GGCTGCACGC 
GGTGTGGCCG CCAGGCCTGG CACATTGCAT GACTTCCGTA GCCATGAGAT CGCAGACCAG 
CTGACGCTCC TCGATGCAGA GCTCTTTTAT AAGATAGAGA TCCCGGAGGT TTTACTTTGG 
GCAAAAGAAC AGAATGAGGA GAAAAGCCCC AACCTGACCC AATTCACAGA GCACTTCAAC 
AACATGTCGT ACTGGGTTCG GTCCATAATA ATCCTGCAGG AGAAAGCCCA GGATCGGGAA 
AAACTTCTCC TGAAGTTCAT CAAAATAATG AAGCACTTAA GGAAGCTCAA TAACTTCAAC 
TCATACTTGG CAATATTATC AGCGCTGGAT TCAGCTCCTA TCAGAAGACT GGAATGGCAG 
AAGCAAACGT CTGAGGGTTT GGAGGAATAT TGCACTTTAA TTGACAGTTC GTCCTCTTTC 
CGGGCGTACA GAGCAGCTTT GGCAGACGTG GAGCCTCCAT GTATACCGTA CCTAGGTCTC 
ATCCTCCAGG ACTTAACGTT TGTGCATCTT GGGAATCCTG ACCACATTGA AGGAAAGATC 
AACTTTTCCA AACGCTGGCA GCAGTTTAAT ATTTTAGACA CTATGAGGCG TTTCCAGCAA 
GTGCACTATG ACCTCAAACA CAACGATGAC ATCGTCTCCT TCTTCAACGA CTTCAGTGAT 
CATCTGGCCG AGGAGGCATT GTGGGAGCTT TCCTTAAAGA TCAAACCCCG CAACATCACC 
AGGCGAAAAA CAGAGCGAGA GGAAAAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302065.1
CDS	157..3249
Translation	MSGKIDSKQESQRSHLTSFTMKLKDKFHSPKIKRTPSNKKSKPPPEQPVKSPEKTVTKSLSRLEEHEKEVVSALRYFKTIIDKMAVDKKVLEMLPGSASKVLEAVTGLVQLDSKILSSSRVSSCLNRLYQSLANLIRWSDQVMLEGVNLEDKDTITTVTNVIKAVLDGVKELVKVAIEKQEHPSPTTPVKTIPPVASPQRVPEPPVIETKEPILNDSPAPAENIETPNDVPEEEVAPPKPPLPGLKVPEQHCPPALPPKKRQSAPSPTRIAVVAPMSRTTSGSSLPIGICRQEYEADALQRRFSGGSHSYGGESPRLSPCGSIGILSKSDEQLSSLDQDSGQCSRNTSCETLDTAESYDPDYDFLHQDLSSTEQIPHTHTGGCLSPLPESLHESSSFPVQHQSMPPALPKKERRPPLPPVERLYSQYDNVPDEDMHTPPFPLFGAIPPSNPGVFMGNFGPSENTQIPQSPPPLPEKKSRHILKYMQFMEDYSEPLPSVFYQTPQSESIYEQRNRKRFQEVYGLSDSFSSSDETLPPPALPPKQRQLSESANDEGGEGEYVNLYSSNQANGDVTLSNGDSFCGAEDSPQDSAIEHSKDPADREGWKQKCVDSDLTSEEVDELCLIDHDEIMSRITLKQENDDGPDVRAGSGDILLVHATETDRKDRVLYCEAFLTTYRTFLTPEDLIKKLRYRYTRFSLSSDSIKQRASKNTFFVLVRVVDELCLVELTEDILKQLMELVFNLLCKGELTLARVLRKNILDKVQQKKLLQYTNSLKTLAARGVAARPGTLHDFRSHEIADQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIILQEKAQDREKLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLEEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDHIEGKINFSKRWQQFNILDTMRRFQQVHYDLKHNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTEREEKT

Target ORF information:

RefSeq Version	XM_009303790.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303790.3

ORF Insert Sequence:

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGAG CGAAAGTGCT AATGATGAAG GTGGGGAGGG TGAATACGTC 
AATCTGTATT CATCCAACCA GGCCAATGGA GATGTGACTC TCTCCAATGG AGACTCTTTT 
TGCGGTGCTG AAGATTCGCC CCAGGACTCC GCTATTGAAC ACAGCAAAGA TCCAGCTGAC 
AGAGAGGGCT GGAAGCAGAA GTGTGTGGAC TCAGATTTGA CCTCAGAAGA GGTGGATGAG 
CTCTGTTTGA TTGACCACGA TGAGATCATG TCTCGCATAA CACTAAAGCA AGAGAACGAC 
GACGGCCCTG ATGTTAGAGC GGGATCAGGC GATATTTTAT TAGTTCATGC CACTGAAACC 
GACCGCAAAG ATCGGGTTTT ATACTGTGAA GCTTTCTTGA CCACGTACAG AACATTTCTA 
ACCCCAGAGG ACCTCATTAA AAAGCTCCGC TATAGGTACA CCAGATTTTC TCTCAGCTCT 
GACAGCATCA AACAACGAGC TAGTAAAAAC ACCTTCTTCG TGCTGGTGCG TGTCGTGGAT 
GAGCTTTGTT TGGTGGAGCT GACGGAAGAC ATCCTGAAGC AGCTCATGGA GCTTGTGTTC 
AATCTCTTGT GTAAAGGGGA GCTTACTTTG GCCCGGGTTC TGCGCAAAAA CATCCTGGAT 
AAAGTCCAGC AGAAGAAGTT GCTGCAGTAC ACTAATTCCC TCAAAACACT GGCTGCACGC 
GGTGTGGCCG CCAGGCCTGG CACATTGCAT GACTTCCGTA GCCATGAGAT CGCAGACCAG 
CTGACGCTCC TCGATGCAGA GCTCTTTTAT AAGATAGAGA TCCCGGAGGT TTTACTTTGG 
GCAAAAGAAC AGAATGAGGA GAAAAGCCCC AACCTGACCC AATTCACAGA GCACTTCAAC 
AACATGTCGT ACTGGGTTCG GTCCATAATA ATCCTGCAGG AGAAAGCCCA GGATCGGGAA 
AAACTTCTCC TGAAGTTCAT CAAAATAATG AAGCACTTAA GGAAGCTCAA TAACTTCAAC 
TCATACTTGG CAATATTATC AGCGCTGGAT TCAGCTCCTA TCAGAAGACT GGAATGGCAG 
AAGCAAACGT CTGAGGGTTT GGAGGAATAT TGCACTTTAA TTGACAGTTC GTCCTCTTTC 
CGGGCGTACA GAGCAGCTTT GGCAGACGTG GAGCCTCCAT GTATACCGTA CCTAGGTCTC 
ATCCTCCAGG ACTTAACGTT TGTGCATCTT GGGAATCCTG ACCACATTGA AGGAAAGATC 
AACTTTTCCA AACGCTGGCA GCAGTTTAAT ATTTTAGACA CTATGAGGCG TTTCCAGCAA 
GTGCACTATG ACCTCAAACA CAACGATGAC ATCGTCTCCT TCTTCAACGA CTTCAGTGAT 
CATCTGGCCG AGGAGGCATT GTGGGAGCTT TCCTTAAAGA TCAAACCCCG CAACATCACC 
AGGCGAAAAA CAGAGCGAGA GGAAAAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1a ( XM_009303790.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302065.1 Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X3, mRNA.

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CloneID	ODa42889
Clone ID Related Accession (Same CDS sequence)	XM_009303790.3 , XM_009303790.2
Accession Version	XM_009303790.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303790.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGAG CGAAAGTGCT AATGATGAAG GTGGGGAGGG TGAATACGTC 
AATCTGTATT CATCCAACCA GGCCAATGGA GATGTGACTC TCTCCAATGG AGACTCTTTT 
TGCGGTGCTG AAGATTCGCC CCAGGACTCC GCTATTGAAC ACAGCAAAGA TCCAGCTGAC 
AGAGAGGGCT GGAAGCAGAA GTGTGTGGAC TCAGATTTGA CCTCAGAAGA GGTGGATGAG 
CTCTGTTTGA TTGACCACGA TGAGATCATG TCTCGCATAA CACTAAAGCA AGAGAACGAC 
GACGGCCCTG ATGTTAGAGC GGGATCAGGC GATATTTTAT TAGTTCATGC CACTGAAACC 
GACCGCAAAG ATCGGGTTTT ATACTGTGAA GCTTTCTTGA CCACGTACAG AACATTTCTA 
ACCCCAGAGG ACCTCATTAA AAAGCTCCGC TATAGGTACA CCAGATTTTC TCTCAGCTCT 
GACAGCATCA AACAACGAGC TAGTAAAAAC ACCTTCTTCG TGCTGGTGCG TGTCGTGGAT 
GAGCTTTGTT TGGTGGAGCT GACGGAAGAC ATCCTGAAGC AGCTCATGGA GCTTGTGTTC 
AATCTCTTGT GTAAAGGGGA GCTTACTTTG GCCCGGGTTC TGCGCAAAAA CATCCTGGAT 
AAAGTCCAGC AGAAGAAGTT GCTGCAGTAC ACTAATTCCC TCAAAACACT GGCTGCACGC 
GGTGTGGCCG CCAGGCCTGG CACATTGCAT GACTTCCGTA GCCATGAGAT CGCAGACCAG 
CTGACGCTCC TCGATGCAGA GCTCTTTTAT AAGATAGAGA TCCCGGAGGT TTTACTTTGG 
GCAAAAGAAC AGAATGAGGA GAAAAGCCCC AACCTGACCC AATTCACAGA GCACTTCAAC 
AACATGTCGT ACTGGGTTCG GTCCATAATA ATCCTGCAGG AGAAAGCCCA GGATCGGGAA 
AAACTTCTCC TGAAGTTCAT CAAAATAATG AAGCACTTAA GGAAGCTCAA TAACTTCAAC 
TCATACTTGG CAATATTATC AGCGCTGGAT TCAGCTCCTA TCAGAAGACT GGAATGGCAG 
AAGCAAACGT CTGAGGGTTT GGAGGAATAT TGCACTTTAA TTGACAGTTC GTCCTCTTTC 
CGGGCGTACA GAGCAGCTTT GGCAGACGTG GAGCCTCCAT GTATACCGTA CCTAGGTCTC 
ATCCTCCAGG ACTTAACGTT TGTGCATCTT GGGAATCCTG ACCACATTGA AGGAAAGATC 
AACTTTTCCA AACGCTGGCA GCAGTTTAAT ATTTTAGACA CTATGAGGCG TTTCCAGCAA 
GTGCACTATG ACCTCAAACA CAACGATGAC ATCGTCTCCT TCTTCAACGA CTTCAGTGAT 
CATCTGGCCG AGGAGGCATT GTGGGAGCTT TCCTTAAAGA TCAAACCCCG CAACATCACC 
AGGCGAAAAA CAGAGCGAGA GGAAAAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302065.1
CDS	151..3243
Translation	MSGKIDSKQESQRSHLTSFTMKLKDKFHSPKIKRTPSNKKSKPPPEQPVKSPEKTVTKSLSRLEEHEKEVVSALRYFKTIIDKMAVDKKVLEMLPGSASKVLEAVTGLVQLDSKILSSSRVSSCLNRLYQSLANLIRWSDQVMLEGVNLEDKDTITTVTNVIKAVLDGVKELVKVAIEKQEHPSPTTPVKTIPPVASPQRVPEPPVIETKEPILNDSPAPAENIETPNDVPEEEVAPPKPPLPGLKVPEQHCPPALPPKKRQSAPSPTRIAVVAPMSRTTSGSSLPIGICRQEYEADALQRRFSGGSHSYGGESPRLSPCGSIGILSKSDEQLSSLDQDSGQCSRNTSCETLDTAESYDPDYDFLHQDLSSTEQIPHTHTGGCLSPLPESLHESSSFPVQHQSMPPALPKKERRPPLPPVERLYSQYDNVPDEDMHTPPFPLFGAIPPSNPGVFMGNFGPSENTQIPQSPPPLPEKKSRHILKYMQFMEDYSEPLPSVFYQTPQSESIYEQRNRKRFQEVYGLSDSFSSSDETLPPPALPPKQRQLSESANDEGGEGEYVNLYSSNQANGDVTLSNGDSFCGAEDSPQDSAIEHSKDPADREGWKQKCVDSDLTSEEVDELCLIDHDEIMSRITLKQENDDGPDVRAGSGDILLVHATETDRKDRVLYCEAFLTTYRTFLTPEDLIKKLRYRYTRFSLSSDSIKQRASKNTFFVLVRVVDELCLVELTEDILKQLMELVFNLLCKGELTLARVLRKNILDKVQQKKLLQYTNSLKTLAARGVAARPGTLHDFRSHEIADQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIILQEKAQDREKLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLEEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDHIEGKINFSKRWQQFNILDTMRRFQQVHYDLKHNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTEREEKT

Target ORF information:

RefSeq Version	XM_009303790.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rap guanine nucleotide exchange factor (GEF) 1a (rapgef1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303790.2

ORF Insert Sequence:

ATGTCTGGGA AAATAGACAG TAAGCAAGAG TCCCAGCGCT CCCACTTGAC ATCCTTCACC 
ATGAAACTAA AGGACAAATT CCACTCTCCC AAAATCAAGA GGACACCATC AAACAAAAAG 
TCCAAACCAC CACCAGAACA ACCCGTAAAG TCCCCAGAGA AAACTGTAAC TAAGAGTCTG 
AGTCGATTAG AGGAGCATGA GAAAGAAGTG GTCAGCGCCT TGCGGTACTT CAAGACCATT 
ATTGACAAGA TGGCGGTGGA CAAGAAGGTG TTAGAAATGC TTCCTGGTTC TGCTAGTAAA 
GTTTTGGAGG CTGTCACAGG CTTGGTACAA CTAGATAGCA AGATACTGAG CAGTTCAAGG 
GTGTCCTCCT GTCTCAACCG ACTCTATCAA AGCCTTGCAA ACCTCATTCG CTGGTCGGAC 
CAAGTAATGC TGGAGGGTGT GAATTTGGAA GATAAAGACA CAATAACAAC AGTCACAAAT 
GTGATCAAAG CAGTGCTGGA TGGTGTAAAG GAGCTGGTTA AAGTTGCCAT TGAGAAGCAG 
GAGCACCCTT CACCTACGAC ACCTGTCAAA ACCATTCCTC CAGTTGCCAG CCCACAGCGT 
GTCCCTGAGC CCCCTGTCAT TGAGACGAAG GAACCCATCT TGAATGACAG CCCTGCCCCA 
GCTGAGAACA TTGAGACCCC AAATGATGTA CCTGAGGAGG AAGTAGCCCC TCCGAAACCC 
CCTCTACCTG GACTCAAAGT GCCTGAACAA CACTGTCCAC CGGCTCTCCC TCCAAAGAAG 
CGTCAGTCGG CACCGTCTCC CACCAGGATT GCTGTGGTTG CGCCAATGAG TCGCACCACA 
AGTGGATCAA GTCTTCCCAT TGGTATCTGT CGACAGGAGT ATGAAGCAGA TGCCTTACAA 
AGGCGTTTTT CTGGAGGCAG TCACTCATAT GGAGGAGAAT CGCCACGACT GTCCCCATGT 
GGCAGCATCG GCATATTGAG CAAGTCAGAT GAACAGCTGT CCTCTCTGGA TCAGGACAGT 
GGACAGTGCT CTCGTAACAC CAGCTGTGAG ACTCTAGACA CAGCAGAGAG CTACGACCCT 
GACTATGACT TCTTGCATCA AGACCTGTCC AGCACTGAGC AGATCCCTCA CACACACACA 
GGGGGTTGTC TGAGCCCCCT CCCCGAATCT CTCCACGAAT CTTCCTCATT TCCAGTCCAG 
CACCAGTCGA TGCCTCCTGC CCTCCCGAAG AAGGAACGGC GGCCACCTCT GCCTCCAGTG 
GAACGACTCT ACTCTCAATA TGACAACGTT CCAGATGAGG ACATGCACAC TCCACCCTTC 
CCTCTCTTCG GTGCAATCCC CCCCTCAAAC CCTGGAGTAT TTATGGGGAA CTTCGGTCCC 
AGTGAGAACA CACAGATCCC CCAGAGCCCC CCACCCCTGC CGGAGAAGAA GAGTCGCCAT 
ATCCTGAAAT ACATGCAGTT CATGGAGGAC TATTCAGAGC CGCTGCCTTC CGTTTTCTAT 
CAGACGCCAC AAAGTGAAAG CATTTATGAG CAGCGCAATA GGAAGCGATT TCAGGAAGTG 
TATGGCCTCA GCGACTCCTT CAGCAGCAGC GATGAGACTC TTCCTCCGCC AGCACTGCCC 
CCAAAACAGA GACAACTGAG CGAAAGTGCT AATGATGAAG GTGGGGAGGG TGAATACGTC 
AATCTGTATT CATCCAACCA GGCCAATGGA GATGTGACTC TCTCCAATGG AGACTCTTTT 
TGCGGTGCTG AAGATTCGCC CCAGGACTCC GCTATTGAAC ACAGCAAAGA TCCAGCTGAC 
AGAGAGGGCT GGAAGCAGAA GTGTGTGGAC TCAGATTTGA CCTCAGAAGA GGTGGATGAG 
CTCTGTTTGA TTGACCACGA TGAGATCATG TCTCGCATAA CACTAAAGCA AGAGAACGAC 
GACGGCCCTG ATGTTAGAGC GGGATCAGGC GATATTTTAT TAGTTCATGC CACTGAAACC 
GACCGCAAAG ATCGGGTTTT ATACTGTGAA GCTTTCTTGA CCACGTACAG AACATTTCTA 
ACCCCAGAGG ACCTCATTAA AAAGCTCCGC TATAGGTACA CCAGATTTTC TCTCAGCTCT 
GACAGCATCA AACAACGAGC TAGTAAAAAC ACCTTCTTCG TGCTGGTGCG TGTCGTGGAT 
GAGCTTTGTT TGGTGGAGCT GACGGAAGAC ATCCTGAAGC AGCTCATGGA GCTTGTGTTC 
AATCTCTTGT GTAAAGGGGA GCTTACTTTG GCCCGGGTTC TGCGCAAAAA CATCCTGGAT 
AAAGTCCAGC AGAAGAAGTT GCTGCAGTAC ACTAATTCCC TCAAAACACT GGCTGCACGC 
GGTGTGGCCG CCAGGCCTGG CACATTGCAT GACTTCCGTA GCCATGAGAT CGCAGACCAG 
CTGACGCTCC TCGATGCAGA GCTCTTTTAT AAGATAGAGA TCCCGGAGGT TTTACTTTGG 
GCAAAAGAAC AGAATGAGGA GAAAAGCCCC AACCTGACCC AATTCACAGA GCACTTCAAC 
AACATGTCGT ACTGGGTTCG GTCCATAATA ATCCTGCAGG AGAAAGCCCA GGATCGGGAA 
AAACTTCTCC TGAAGTTCAT CAAAATAATG AAGCACTTAA GGAAGCTCAA TAACTTCAAC 
TCATACTTGG CAATATTATC AGCGCTGGAT TCAGCTCCTA TCAGAAGACT GGAATGGCAG 
AAGCAAACGT CTGAGGGTTT GGAGGAATAT TGCACTTTAA TTGACAGTTC GTCCTCTTTC 
CGGGCGTACA GAGCAGCTTT GGCAGACGTG GAGCCTCCAT GTATACCGTA CCTAGGTCTC 
ATCCTCCAGG ACTTAACGTT TGTGCATCTT GGGAATCCTG ACCACATTGA AGGAAAGATC 
AACTTTTCCA AACGCTGGCA GCAGTTTAAT ATTTTAGACA CTATGAGGCG TTTCCAGCAA 
GTGCACTATG ACCTCAAACA CAACGATGAC ATCGTCTCCT TCTTCAACGA CTTCAGTGAT 
CATCTGGCCG AGGAGGCATT GTGGGAGCTT TCCTTAAAGA TCAAACCCCG CAACATCACC 
AGGCGAAAAA CAGAGCGAGA GGAAAAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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rapgef1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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