dnaaf4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dnaaf4
Entrez Gene ID	402988
Full Name	dynein axonemal assembly factor 4
Synonyms	EKN1,dyx1c1,zgc:77853
General protein information	Preferred Names dynein axonemal assembly factor 4 Names dyslexia susceptibility 1 candidate gene 1 protein dyslexia susceptibility 1 candidate 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205688.1	NP_991251.1	dyslexia susceptibility 1 candidate gene 1 protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DYX1C1	NP_001009083.1
Mus musculus (house mouse)	Dyx1c1	NP_080590.3
Danio rerio (zebrafish)	dyx1c1	NP_991251.1
Xenopus tropicalis (tropical clawed frog)	dyx1c1	NP_001011174.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001727	XP_001238468.1
Homo sapiens (human)	DYX1C1	NP_570722.2
Bos taurus (cattle)	DYX1C1	NP_001193117.1
Canis lupus familiaris (dog)	DYX1C1	XP_005638492.1
Rattus norvegicus (Norway rat)	Dyx1c1	NP_001007011.1
Gallus gallus (chicken)	DYX1C1	NP_001008674.1

Gene Ontology
Bibliography

Related articles in PubMed

The Progress of CRISPR/Cas9-Mediated Gene Editing in Generating Mouse/Zebrafish Models of Human Skeletal Diseases.
Wu N, Liu B, Du H, Zhao S, Li Y, Cheng X, Wang S, Lin J, Zhou J, , Qiu G, Wu Z, Zhang J
Computational and structural biotechnology journal17954-962(2019)

Zebrafish models of idiopathic scoliosis link cerebrospinal fluid flow defects to spine curvature.
Grimes DT, Boswell CW, Morante NF, Henkelman RM, Burdine RD, Ciruna B
Science (New York, N.Y.)352(6291)1341-4(2016 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Systematic discovery of novel ciliary genes through functional genomics in the zebrafish.
Choksi SP, Babu D, Lau D, Yu X, Roy S
Development (Cambridge, England)141(17)3410-9(2014 Sep)

DYX1C1 is required for axonemal dynein assembly and ciliary motility.
Tarkar A, Loges NT, Slagle CE, Francis R, Dougherty GW, Tamayo JV, Shook B, Cantino M, Schwartz D, Jahnke C, Olbrich H, Werner C, Raidt J, Pennekamp P, Abouhamed M, Hjeij R, Köhler G, Griese M, Li Y, Lemke K, Klena N, Liu X, Gabriel G, Tobita K, Jaspers M, Morgan LC, Shapiro AJ, Letteboer SJ, Mans DA, Carson JL, Leigh MW, Wolf WE, Chen S, Lucas JS, Onoufriadis A, Plagnol V, Schmidts M, Boldt K, , Roepman R, Zariwala MA, Lo CW, Mitchison HM, Knowles MR, Burdine RD, Loturco JJ, Omran H
Nature genetics45(9)995-1003(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dnaaf4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dnaaf4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03968	NM_205688.1 Latest version!	Danio rerio dynein axonemal assembly factor 4 (dnaaf4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dnaaf4 ( NM_205688.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991251.1 Danio rerio dynein axonemal assembly factor 4 (dnaaf4), mRNA.

Price & Availability↑

CloneID	ODa03968
Clone ID Related Accession (Same CDS sequence)	NM_205688.1
Accession Version	NM_205688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576252800000
Organism	Danio rerio(zebrafish)
Product	dyslexia susceptibility 1 candidate gene 1 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC065881.1. ##Evidence-Data-START## Transcript exon combination :: BC065881.1, AY428510.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCGCTGA TAGTAAGAGA TCACACATGG ACACAAAACC TGAGCACAGT TTACATCAGC 
GTCCCTTTAA AAGCAGTGAA AACCGCAAAT GTACACATCA TCTGCACGGA CGACTACCTG 
AAGGTCAGTT TCCCACCGTT TCTCTTCGAG GTTTTCTTAT TCGCCTCAAT AAATGAGGAG 
AAAAGTGAAG CCAGGATTGG AAACGGTGCT GCAGTTTTCA CTCTTCAGAA GAGGAGAGAT 
GAAATGTGGG AGCAGCTCTG TACAAACATC GATAAAGAGA AACAGAAGCA AATTCGAGAA 
CAAGCCGTTC TCAAAGTTCA GGAGAAAGCA GTGGAAAAGT CTAAAACCAA AGCCACCCGG 
ATTCAACAGG AGAAGAAGTA TGCGCTGGAA ACCATGATGA AGCTTGAGAG CGAGGAGCGG 
GAGAGGATTC AGAAGAGGAA GGATGAAGTG TGTGCGAGAG CCACAGCAGA GCTGGATTTC 
TGGAGAGAAA CACAGAGAAA AACAGCCAAG GGAAACGAGA ACCAGATAAA AGAGCAGAGA 
ACCAGCACAC ATGACAACAA ACCGGTTCCT CAGAAAATAA AGCATGCAAA CACTTCACCT 
GCAGACACAG CATTGGTGAA AACCGACAAC ACTATCACTG GTCAGATGAG GAGTAAACAG 
AAAACAAAGG ATCTTCCCGC TCCCAGATCT GCTGGCTGTA TTCAGATCAG CTTCACTCCA 
CGAGTGTTTC CCACCGCGCT CAGAGAGTCT CGCGTCCCCG AGGAGGAGGA GTGGTTGAAG 
AAGCAGGCGG AGGCCAGGAG AGCAATAGAC ACAGATCTGG CAGAGCTGGA CGACCTGACA 
GAGGACGAGA GAAACCCAGA CTGGCTGAAA GACAAGGGAG ATAAATTGTT CATGGCAGGA 
AACTTTCTGG CTGCTGTCAA CGCTTATAAT CTGGCCATAA AACTCAACAG GAAGATGCCG 
GCTTTATTTT CTAATCGAGC GGCTTGTCAC CTCAAACTGA GAAATCTCCA CAAGGCCATT 
GAGGACAGCT CTCAGGCGCT TGAGTTGCTG ACGCCTGCGG TCTCTGCGAA CGCTTCAGCT 
CGACTGAAGG CTCATGTGAG GCGGGGGACA GCCTTCTGTC AGCTCCAGCT CTATGTGGAA 
GGTCTTCAAG ACTATCAGAA GGCTCTAGAA ATGGACCCAC ACAATGCAGC ACTGCGAGCC 
GATACCGAGC AGATCCGTGA GCTCATACAG GGATCCACTC CACGATCTAA AGATGAAAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991251.1
CDS	19..1281
Translation	MPLIVRDHTWTQNLSTVYISVPLKAVKTANVHIICTDDYLKVSFPPFLFEVFLFASINEEKSEARIGNGAAVFTLQKRRDEMWEQLCTNIDKEKQKQIREQAVLKVQEKAVEKSKTKATRIQQEKKYALETMMKLESEERERIQKRKDEVCARATAELDFWRETQRKTAKGNENQIKEQRTSTHDNKPVPQKIKHANTSPADTALVKTDNTITGQMRSKQKTKDLPAPRSAGCIQISFTPRVFPTALRESRVPEEEEWLKKQAEARRAIDTDLAELDDLTEDERNPDWLKDKGDKLFMAGNFLAAVNAYNLAIKLNRKMPALFSNRAACHLKLRNLHKAIEDSSQALELLTPAVSANASARLKAHVRRGTAFCQLQLYVEGLQDYQKALEMDPHNAALRADTEQIRELIQGSTPRSKDEN

Target ORF information:

RefSeq Version	NM_205688.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dynein axonemal assembly factor 4 (dnaaf4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205688.1

ORF Insert Sequence:

ATGCCGCTGA TAGTAAGAGA TCACACATGG ACACAAAACC TGAGCACAGT TTACATCAGC 
GTCCCTTTAA AAGCAGTGAA AACCGCAAAT GTACACATCA TCTGCACGGA CGACTACCTG 
AAGGTCAGTT TCCCACCGTT TCTCTTCGAG GTTTTCTTAT TCGCCTCAAT AAATGAGGAG 
AAAAGTGAAG CCAGGATTGG AAACGGTGCT GCAGTTTTCA CTCTTCAGAA GAGGAGAGAT 
GAAATGTGGG AGCAGCTCTG TACAAACATC GATAAAGAGA AACAGAAGCA AATTCGAGAA 
CAAGCCGTTC TCAAAGTTCA GGAGAAAGCA GTGGAAAAGT CTAAAACCAA AGCCACCCGG 
ATTCAACAGG AGAAGAAGTA TGCGCTGGAA ACCATGATGA AGCTTGAGAG CGAGGAGCGG 
GAGAGGATTC AGAAGAGGAA GGATGAAGTG TGTGCGAGAG CCACAGCAGA GCTGGATTTC 
TGGAGAGAAA CACAGAGAAA AACAGCCAAG GGAAACGAGA ACCAGATAAA AGAGCAGAGA 
ACCAGCACAC ATGACAACAA ACCGGTTCCT CAGAAAATAA AGCATGCAAA CACTTCACCT 
GCAGACACAG CATTGGTGAA AACCGACAAC ACTATCACTG GTCAGATGAG GAGTAAACAG 
AAAACAAAGG ATCTTCCCGC TCCCAGATCT GCTGGCTGTA TTCAGATCAG CTTCACTCCA 
CGAGTGTTTC CCACCGCGCT CAGAGAGTCT CGCGTCCCCG AGGAGGAGGA GTGGTTGAAG 
AAGCAGGCGG AGGCCAGGAG AGCAATAGAC ACAGATCTGG CAGAGCTGGA CGACCTGACA 
GAGGACGAGA GAAACCCAGA CTGGCTGAAA GACAAGGGAG ATAAATTGTT CATGGCAGGA 
AACTTTCTGG CTGCTGTCAA CGCTTATAAT CTGGCCATAA AACTCAACAG GAAGATGCCG 
GCTTTATTTT CTAATCGAGC GGCTTGTCAC CTCAAACTGA GAAATCTCCA CAAGGCCATT 
GAGGACAGCT CTCAGGCGCT TGAGTTGCTG ACGCCTGCGG TCTCTGCGAA CGCTTCAGCT 
CGACTGAAGG CTCATGTGAG GCGGGGGACA GCCTTCTGTC AGCTCCAGCT CTATGTGGAA 
GGTCTTCAAG ACTATCAGAA GGCTCTAGAA ATGGACCCAC ACAATGCAGC ACTGCGAGCC 
GATACCGAGC AGATCCGTGA GCTCATACAG GGATCCACTC CACGATCTAA AGATGAAAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Progress of CRISPR/Cas9-Mediated Gene Editing in Generating Mouse/Zebrafish Models of Human Skeletal Diseases.
Computational and structural biotechnology journal17954-962(2019)
Wu N,Liu B,Du H,Zhao S,Li Y,Cheng X,Wang S,Lin J,Zhou J,,Qiu G,Wu Z,Zhang J

Zebrafish models of idiopathic scoliosis link cerebrospinal fluid flow defects to spine curvature.
Science (New York, N.Y.)352(6291)1341-4(2016 Jun)
Grimes DT,Boswell CW,Morante NF,Henkelman RM,Burdine RD,Ciruna B

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Systematic discovery of novel ciliary genes through functional genomics in the zebrafish.
Development (Cambridge, England)141(17)3410-9(2014 Sep)
Choksi SP,Babu D,Lau D,Yu X,Roy S

DYX1C1 is required for axonemal dynein assembly and ciliary motility.
Nature genetics45(9)995-1003(2013 Sep)
Tarkar A,Loges NT,Slagle CE,Francis R,Dougherty GW,Tamayo JV,Shook B,Cantino M,Schwartz D,Jahnke C,Olbrich H,Werner C,Raidt J,Pennekamp P,Abouhamed M,Hjeij R,Köhler G,Griese M,Li Y,Lemke K,Klena N,Liu X,Gabriel G,Tobita K,Jaspers M,Morgan LC,Shapiro AJ,Letteboer SJ,Mans DA,Carson JL,Leigh MW,Wolf WE,Chen S,Lucas JS,Onoufriadis A,Plagnol V,Schmidts M,Boldt K,,Roepman R,Zariwala MA,Lo CW,Mitchison HM,Knowles MR,Burdine RD,Loturco JJ,Omran H

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

dnaaf4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM