lrrc8aa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lrrc8aa
Entrez Gene ID	327642
Full Name	leucine rich repeat containing 8 VRAC subunit Aa
Synonyms	lrrc8a,wu:fb18g12,wu:fi21b10
General protein information	Preferred Names leucine rich repeat containing 8 VRAC subunit Aa Names volume-regulated anion channel subunit LRRC8A leucine rich repeat containing 8 family, member A leucine rich repeat containing 8 family, member Aa
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001029949.1	NP_001025120.1	volume-regulated anion channel subunit LRRC8A

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8A	NP_001120717.1
Bos taurus (cattle)	LRRC8A	NP_001070275.1
Danio rerio (zebrafish)	lrrc8a	NP_001025120.1
Danio rerio (zebrafish)	si:ch211-236k19.7	XP_005165659.1
Xenopus tropicalis (tropical clawed frog)	lrrc8a	XP_004916747.1
Macaca mulatta (Rhesus monkey)	LRRC8A	XP_001109698.1
Mus musculus (house mouse)	Lrrc8a	NP_808393.1
Gallus gallus (chicken)	LRRC8A	XP_415484.1
Gallus gallus (chicken)	LOC100858917	XP_003642331.1
Pan troglodytes (chimpanzee)	LRRC8A	XP_520301.1
Canis lupus familiaris (dog)	LRRC8A	XP_005625321.1
Rattus norvegicus (Norway rat)	Lrrc8a	NP_001019953.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Leucine-rich repeat containing protein LRRC8A is essential for swelling-activated Cl- currents and embryonic development in zebrafish.
Yamada T, Wondergem R, Morrison R, Yin VP, Strange K
Physiological reports4(19)(2016 10)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Baraban SC, Dinday MT, Hortopan GA
Nature communications42410(2013)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following lrrc8aa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrrc8aa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00734	NM_001029949.1 Latest version!	Danio rerio leucine rich repeat containing 8 VRAC subunit Aa (lrrc8aa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lrrc8aa ( NM_001029949.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025120.1 Danio rerio leucine rich repeat containing 8 VRAC subunit Aa (lrrc8aa), mRNA.

Price & Availability↑

CloneID	ODa00734
Clone ID Related Accession (Same CDS sequence)	NM_001029949.1
Accession Version	NM_001029949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	volume-regulated anion channel subunit LRRC8A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC097172.1. On Jul 30, 2005 this sequence version replaced XM_683323.1. ##Evidence-Data-START## Transcript exon combination :: BC097172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATTCCCA TCACTGAGCT GCGGTATTTC GCGGACACCC AGCCGGCATA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT CACCGACTAC ATTTCCATCG TCATGCTGAT GATCGCGGTG 
TTCGGCGGGA CGCTCCAGGT CACCCAGGAC AAGATGATCT GCTTGCCCTG CAAGTGGGTG 
GTCAACCAGA CTTGCAAACA CCACCTCAAC GTCAGCGTCC CGCTTCACAC CGAGCCCAAA 
GGCATCCAAT ACGACCTCGA CCGGCACCAG TACAACTACG TGGACGCCGT GTGCTACGAA 
AACAAGCTGC ACTGGTTCGC TAAATACTTC CCATACCTGG TGCTCCTCCA CACACTCATC 
TTTTTGGCCT GCAGCAACTT CTGGTTCAAA TTTCCACGCA CCAGTTCCAA GCTGGAGCAT 
TTCGTGTCCA TTCTGCTGAA GTGTTTCGAT TCGCCCTGGA CGACCCGAGC ACTTTCTGAG 
ACCGTAGTAG AGGAGAGCGA TCCCAAGCCG GCAGGGAAGA TGAACGGCTC CATGGATAAG 
AAAGCCTCCA GTGTTAGCGA TCAGGACGTG GAGGCGAGCG TCCCAATGCT GCAGAGGACC 
AAATCCAGGA TCGAGCAGGG CATCGTGGAT CATTCGGAGA CCGGCGTGCT GGACAAGAAG 
GAAGGAGAGC AGGCCAAAGC GCTGTTTGAG AAGGTGAAAA AGTTTCGCAT CCATGTGGAG 
GAGGGCGACA TCGTCCACCG TCTCTACATC CGCCAAATCA TCATCAAAGT CATCCAGTTC 
CTCATCATCA TCTGCTACAC CATGTACTAC GTGCACTACA TCACCTTCAG CGTCACCTGC 
ACAGTGGACA TCGAGAGACT GACCGGATAT CGCACATACC ACTGTGCACA TCCACTAGCC 
ACACTTTTCA AGATCCTAGC CTGCTTCTAC ATCAGCCTGG TGGTGGTTTA CGGCCTCATA 
TGCATGTACA CGCTGTACTG GATCATCAGT CGCTCGCTTA AACGATATTC ATTCGAGTCT 
ATCCGTGAAG AGAGCAGTTA TAGCGACATC CCGGACGTCA AAAACGACTT CGCCTTTATG 
CTGCATATGA TCGATCAGTA CGACCCGCTG TACTCCAAGA GGTTTGCTGT CTTTCTGTCG 
GAGGTCAGTG GGAATAAGCT TCGGCAGCTG AATCTGAATA ACGAGTGGGC ACTGGAGAAA 
CTCCGACAGA GGATCACCAA AAACTCGCAG GAGAAGCTGG AGCTGCATCT GTTTATGCTG 
AGCGGGATTC CTGATACGGT GTTCGACCTG GTGGAGCTGG AGGTTCTGAA ACTGGAGCTG 
ATTCCAGACA TCACGATCCC GCCGATCATC GCACAGCTCT CAAATCTGAG AGAGATGTGG 
CTTTACCACA CGCCGGCCAG AATCGAAGCT CCGGCTCTGG CCTTTCTGCG GGAAAACCTC 
ACATCCCTGC ACATCAAGTT CACAGACATC AAGGAGATTC CGCTGTGGAT CTACAGCCTC 
AAGAACCTCA GCGAGCTCCA TCTGACGGGA AACCTAAGTG CAGAAAACAA CCGATTCATC 
GTCATCGACG GCCTGCGGGA GCTCAAGAGA CTCAAAGTGC TGCGGCTGAA GAGCAACCTC 
ACCAAACTGC CTCAGGTGGT GACAGACGTT GGTGTCCATC TGCAGAAGCT CTCCATCAAT 
AACGAAGGCA CCAAACTGAT GGTCCTGAAC AGTCTGAAGA AGATGGTGAA CCTGACAGAG 
CTGGAGCTGC TGTGCTGTGA TCTGGAGAGG ATCCCGCACT CCATATTCAG CCTGCACAAT 
CTACAGGAAA TTGATCTGAA GGACAACAAC CTGAAGACCA TCGAGGAGAT CATCAGTTTC 
CAGCATCTCC ACAGGCTGGT GTGTTTGAAA CTCTGGTACA ACCAGATCGC CTACATCCCC 
ATCCAGATCG GCACGCTCAC CAACCTGGAG CGCCTATACC TGAACAGAAA CAAAATTGAG 
AAGATCCCGG CTCAGCTGTT CTTCTGCAGG AAGCTGCGTT ATCTGGACCT CAGTCACAAT 
AACCTGACCA GCATTCCTGC AGACATCGGC TTCCTTCAGA ACCTGCAGTA CTTTGCTGTG 
ACCGCCAACA GAATTGAAAC TTTGCCACCT GAGTTGTTCC AGTGTAAGAA GCTGCGGACG 
CTGAATCTGG GAAATAACTG CCTTACCGCT CTGCCATCCC GTTTCGGGGA GTTAAGCGGC 
CTGACGCAGC TGGAGCTCCG CGGGAATCGT CTGGAGGGAT TACCGGTGGA GCTTTCCGAA 
TGCCGCCTCC TGAAGCGCTC GGGTCTGATC GTGGAGGAGG ATCTGTTCAA CACTTTACCT 
CCAGAGCTCA AGGAGCAGCT CTGGAGAACT GATAAAGAAC AAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025120.1
CDS	106..2493
Translation	MIPITELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVVNQTCKHHLNVSVPLHTEPKGIQYDLDRHQYNYVDAVCYENKLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAGKMNGSMDKKASSVSDQDVEASVPMLQRTKSRIEQGIVDHSETGVLDKKEGEQAKALFEKVKKFRIHVEEGDIVHRLYIRQIIIKVIQFLIIICYTMYYVHYITFSVTCTVDIERLTGYRTYHCAHPLATLFKILACFYISLVVVYGLICMYTLYWIISRSLKRYSFESIREESSYSDIPDVKNDFAFMLHMIDQYDPLYSKRFAVFLSEVSGNKLRQLNLNNEWALEKLRQRITKNSQEKLELHLFMLSGIPDTVFDLVELEVLKLELIPDITIPPIIAQLSNLREMWLYHTPARIEAPALAFLRENLTSLHIKFTDIKEIPLWIYSLKNLSELHLTGNLSAENNRFIVIDGLRELKRLKVLRLKSNLTKLPQVVTDVGVHLQKLSINNEGTKLMVLNSLKKMVNLTELELLCCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLVCLKLWYNQIAYIPIQIGTLTNLERLYLNRNKIEKIPAQLFFCRKLRYLDLSHNNLTSIPADIGFLQNLQYFAVTANRIETLPPELFQCKKLRTLNLGNNCLTALPSRFGELSGLTQLELRGNRLEGLPVELSECRLLKRSGLIVEEDLFNTLPPELKEQLWRTDKEQA

Target ORF information:

RefSeq Version	NM_001029949.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine rich repeat containing 8 VRAC subunit Aa (lrrc8aa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029949.1

ORF Insert Sequence:

ATGATTCCCA TCACTGAGCT GCGGTATTTC GCGGACACCC AGCCGGCATA CCGAATCCTG 
AAGCCATGGT GGGACGTGTT CACCGACTAC ATTTCCATCG TCATGCTGAT GATCGCGGTG 
TTCGGCGGGA CGCTCCAGGT CACCCAGGAC AAGATGATCT GCTTGCCCTG CAAGTGGGTG 
GTCAACCAGA CTTGCAAACA CCACCTCAAC GTCAGCGTCC CGCTTCACAC CGAGCCCAAA 
GGCATCCAAT ACGACCTCGA CCGGCACCAG TACAACTACG TGGACGCCGT GTGCTACGAA 
AACAAGCTGC ACTGGTTCGC TAAATACTTC CCATACCTGG TGCTCCTCCA CACACTCATC 
TTTTTGGCCT GCAGCAACTT CTGGTTCAAA TTTCCACGCA CCAGTTCCAA GCTGGAGCAT 
TTCGTGTCCA TTCTGCTGAA GTGTTTCGAT TCGCCCTGGA CGACCCGAGC ACTTTCTGAG 
ACCGTAGTAG AGGAGAGCGA TCCCAAGCCG GCAGGGAAGA TGAACGGCTC CATGGATAAG 
AAAGCCTCCA GTGTTAGCGA TCAGGACGTG GAGGCGAGCG TCCCAATGCT GCAGAGGACC 
AAATCCAGGA TCGAGCAGGG CATCGTGGAT CATTCGGAGA CCGGCGTGCT GGACAAGAAG 
GAAGGAGAGC AGGCCAAAGC GCTGTTTGAG AAGGTGAAAA AGTTTCGCAT CCATGTGGAG 
GAGGGCGACA TCGTCCACCG TCTCTACATC CGCCAAATCA TCATCAAAGT CATCCAGTTC 
CTCATCATCA TCTGCTACAC CATGTACTAC GTGCACTACA TCACCTTCAG CGTCACCTGC 
ACAGTGGACA TCGAGAGACT GACCGGATAT CGCACATACC ACTGTGCACA TCCACTAGCC 
ACACTTTTCA AGATCCTAGC CTGCTTCTAC ATCAGCCTGG TGGTGGTTTA CGGCCTCATA 
TGCATGTACA CGCTGTACTG GATCATCAGT CGCTCGCTTA AACGATATTC ATTCGAGTCT 
ATCCGTGAAG AGAGCAGTTA TAGCGACATC CCGGACGTCA AAAACGACTT CGCCTTTATG 
CTGCATATGA TCGATCAGTA CGACCCGCTG TACTCCAAGA GGTTTGCTGT CTTTCTGTCG 
GAGGTCAGTG GGAATAAGCT TCGGCAGCTG AATCTGAATA ACGAGTGGGC ACTGGAGAAA 
CTCCGACAGA GGATCACCAA AAACTCGCAG GAGAAGCTGG AGCTGCATCT GTTTATGCTG 
AGCGGGATTC CTGATACGGT GTTCGACCTG GTGGAGCTGG AGGTTCTGAA ACTGGAGCTG 
ATTCCAGACA TCACGATCCC GCCGATCATC GCACAGCTCT CAAATCTGAG AGAGATGTGG 
CTTTACCACA CGCCGGCCAG AATCGAAGCT CCGGCTCTGG CCTTTCTGCG GGAAAACCTC 
ACATCCCTGC ACATCAAGTT CACAGACATC AAGGAGATTC CGCTGTGGAT CTACAGCCTC 
AAGAACCTCA GCGAGCTCCA TCTGACGGGA AACCTAAGTG CAGAAAACAA CCGATTCATC 
GTCATCGACG GCCTGCGGGA GCTCAAGAGA CTCAAAGTGC TGCGGCTGAA GAGCAACCTC 
ACCAAACTGC CTCAGGTGGT GACAGACGTT GGTGTCCATC TGCAGAAGCT CTCCATCAAT 
AACGAAGGCA CCAAACTGAT GGTCCTGAAC AGTCTGAAGA AGATGGTGAA CCTGACAGAG 
CTGGAGCTGC TGTGCTGTGA TCTGGAGAGG ATCCCGCACT CCATATTCAG CCTGCACAAT 
CTACAGGAAA TTGATCTGAA GGACAACAAC CTGAAGACCA TCGAGGAGAT CATCAGTTTC 
CAGCATCTCC ACAGGCTGGT GTGTTTGAAA CTCTGGTACA ACCAGATCGC CTACATCCCC 
ATCCAGATCG GCACGCTCAC CAACCTGGAG CGCCTATACC TGAACAGAAA CAAAATTGAG 
AAGATCCCGG CTCAGCTGTT CTTCTGCAGG AAGCTGCGTT ATCTGGACCT CAGTCACAAT 
AACCTGACCA GCATTCCTGC AGACATCGGC TTCCTTCAGA ACCTGCAGTA CTTTGCTGTG 
ACCGCCAACA GAATTGAAAC TTTGCCACCT GAGTTGTTCC AGTGTAAGAA GCTGCGGACG 
CTGAATCTGG GAAATAACTG CCTTACCGCT CTGCCATCCC GTTTCGGGGA GTTAAGCGGC 
CTGACGCAGC TGGAGCTCCG CGGGAATCGT CTGGAGGGAT TACCGGTGGA GCTTTCCGAA 
TGCCGCCTCC TGAAGCGCTC GGGTCTGATC GTGGAGGAGG ATCTGTTCAA CACTTTACCT 
CCAGAGCTCA AGGAGCAGCT CTGGAGAACT GATAAAGAAC AAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Leucine-rich repeat containing protein LRRC8A is essential for swelling-activated Cl- currents and embryonic development in zebrafish.
Physiological reports4(19)(2016 10)
Yamada T,Wondergem R,Morrison R,Yin VP,Strange K

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Nature communications42410(2013)
Baraban SC,Dinday MT,Hortopan GA

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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lrrc8aa cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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