mtor cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mtor
Entrez Gene ID	324254
Full Name	mechanistic target of rapamycin kinase
Synonyms	frap1,tor,wu:fc22h08
General protein information	Preferred Names mechanistic target of rapamycin kinase Names serine/threonine-protein kinase mTOR FK506 binding protein 12-rapamycin associated protein 1 mechanistic target of rapamycin (serine/threonine kinase) target of rapamycin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077211.2	NP_001070679.2	serine/threonine-protein kinase mTOR

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	tor2	NP_595359.2
Homo sapiens (human)	MTOR	NP_004949.1
Canis lupus familiaris (dog)	MTOR	XP_005618060.1
Gallus gallus (chicken)	MTOR	XP_417614.3
Danio rerio (zebrafish)	mtor	NP_001070679.2
Kluyveromyces lactis	KLLA0B13948g	XP_452152.1
Neurospora crassa	NCU05608	XP_960570.1
Pan troglodytes (chimpanzee)	MTOR	XP_003949341.1
Drosophila melanogaster (fruit fly)	Tor	NP_001260427.1
Arabidopsis thaliana (thale cress)	TOR	NP_175425.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007873	XP_317619.4
Bos taurus (cattle)	MTOR	XP_005217047.1
Xenopus tropicalis (tropical clawed frog)	mtor	XP_004916088.1
Rattus norvegicus (Norway rat)	Mtor	NP_063971.1
Oryza sativa (rice)	Os05g0235300	NP_001054988.2
Magnaporthe oryzae (rice blast fungus)	MGG_15156	XP_003710485.1
Mus musculus (house mouse)	Mtor	NP_064393.2
Saccharomyces cerevisiae (baker's yeast)	TOR2	NP_012719.2
Eremothecium gossypii	AGOS_AFR420W	NP_985967.2

Gene Ontology
Bibliography

Related articles in PubMed

Target identification, screening and in vivo evaluation of pyrrolone-fused benzosuberene compounds against human epilepsy using Zebrafish model of pentylenetetrazol-induced seizures.
Tanwar G, Mazumder AG, Bhardwaj V, Kumari S, Bharti R, Yamini, Singh D, Das P, Purohit R
Scientific reports9(1)7904(2019 May)

GSK-3β at the Crossroads in Regulating Protein Synthesis and Lipid Deposition in Zebrafish.
Gu Y, Gao L, Han Q, Li A, Yu H, Liu D, Pang Q
Cells8(3)(2019 02)

Mechanistic toxicity of DEHP at environmentally relevant concentrations (ERCs) and ecological risk assessment in the Three Gorges Reservoir Area, China.
Junaid M, Jia PP, Tang YM, Xiong WX, Huang HY, Strauss PR, Li WG, Pei DS
Environmental pollution (Barking, Essex : 1987)242(Pt B)1939-1949(2018 Nov)

Fertility Enhancement but Premature Ovarian Failure in
Chen Y, Tang H, Wang L, He J, Guo Y, Liu Y, Liu X, Lin H
Frontiers in endocrinology9567(2018)

Inhibited Carnitine Synthesis Causes Systemic Alteration of Nutrient Metabolism in Zebrafish.
Li JM, Li LY, Qin X, Degrace P, Demizieux L, Limbu SM, Wang X, Zhang ML, Li DL, Du ZY
Frontiers in physiology9509(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mtor gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mtor cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36591	NM_001077211.2 Latest version!	Danio rerio mechanistic target of rapamycin kinase (mtor), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mtor ( NM_001077211.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070679.2 Danio rerio mechanistic target of rapamycin kinase (mtor), mRNA.

Price & Availability↑

CloneID	ODa36591
Clone ID Related Accession (Same CDS sequence)	NM_001077211.2
Accession Version	NM_001077211.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573228800000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase mTOR
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ666026.2. On Dec 2, 2006 this sequence version replaced NM_001077211.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ666026.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGGCA CTGCCACAGT TCTGCAGCAG TTTGTGAGTG GCCTGAAGAG TCGCAATGAA 
GACACTCGTG CCAAAGCTGC CAAAGACCTT CAGCACTACG TCACGACAGA ACTAAGAGAG 
CTGAGCCAAG ATGAAGCCAC CACTTTCTAC GATGAACTGA ACCACCACAT TTTTGAGTTG 
GTTTCCAGCT CAGATGTGAA TGAGAAAAAA GGCGGTATCC TTGCCATAGT GAGCCTGATT 
GGAGTGGAGG GAGGAAATGC CACCCGTATC AGCCGCTTTG CCAACTATTT GCGGAACCTG 
CTTCCGTCCA GTGACTCAGT GGTCATGGAG ATGGCCTCTA AAGCCATGGG TCACCTGTCC 
ATGGCGGGAG ACACCTTCAC TGCAGAGTAT GTGGAGTTTG AGGTGAAGAG AGCTCTGGAG 
TGGCTGGGAG CAGACAGGAA TGAAGGCAGA CGACATGCAG CGGTGCTGGT GTTACGAGAA 
CTGGCTGTCA GCGCACCTAC ATTCTTTTTC CAGCAGGTGC AGCCATTCTT TGATAACATT 
TTTTATGCAG TGTGGGACTC CAAACAGGCC ATTCGTGAGG GCGCAGTATC TGCTCTCAGA 
GCTTGTTTGA TCCTTACCAC TCAGAGAGAG ACCAAAGAGA TGCAGAAACC ACAGTGGTAC 
AAGCAAACAT TTGAGGAAGC CGAGAAGGGT TTTGATGAGA CTTTGGCGAA AGAGAAAGGG 
ATGAATAAGG ATGATCGTGT ACATGGAGCT CTACTGATTC TCAATGAGCT AGTGCGAATA 
AGCAGTATGG AGGGAGAGCG TATGAGAGAG GAGATGGAGG AGATCACGCA GCAGCAGTTG 
GTTCATGATA AGTACTGTAA GGAGTTGATG GGTTTCAGCA CTAAGCCTCG TCACATCACG 
CCCTTCACCA GTTTTCAGTC CGTTCAGCCG CAGCAGTCTA ACGCTCTGCT GGGGCTGCTG 
GGTTACAGCA CACCTCAGGG CTTCTTAAGT TTTGGAGCTG CACCTGTTCC CTCGAAAAGC 
ACACTGGTGG AGAGCCGCTA CTGCAGAGAA CTGATGGAGG AGCGCTTTGA CCAGGTGTGC 
CGTTGGGTGC TGAAATACAG GACCAGTAAG AATCCTCTAA TCCAGATGAC AATCTTAAAC 
CTACTGCCTC GACTTGCTGC CTTCCAGCCT CACACTTTCA CTGATCAGTA TTTGCAGGAC 
ACTATGGGGC ACCTGCTGGG CTGCCTAAAA AAAGAAAAGG AGCGGACGGC AGCATTTCAA 
GCGCTGGGGC TACTGGTGGT GGCTGTGAGG GCTGATATTC AGCCATACCT AAGCAAGATC 
CTGGAGATTA TCAAAGCTGC ATTGCCACCG AAAGACTTTG CGCACAAGAG GCAGAAGACC 
ATGCAGGTGG ATGCTACTGT ATTTACTTGC ATAAGTATGC TGTCCAGAGC TATGGGGCCT 
AGCATCCAAC AGGACGTCAA AGAACTTCTG GAGCCCATGC TAGCTGTTGG TCTCAGCCCT 
GCTTTGACTG CAGTGCTTTA CGACCTGAGC AGGCAGATCC CACAGCTGAA GAAGGACATT 
CAGGATGGTC TGCTAAAGAT GCTTTCACTT GTGCTCATGC ACAAGCCTCT TCGTCACCCG 
GGTATGCCCA AGGGCCTGGC GTATCAGCTG TCATCGCCCA GCCTGACCAA CATCCCTGAG 
GCTAGCGACG TGGGCAGCAT AACTCTGGCA CTGAGAACTC TCGGCAGCTT TGAATTTGAA 
GGCCACTCCT TGACCCAGTT TGTGCGTCAC TGTGCGGACC ACTTTTTGAA CAGCGAGCAC 
AAGGAGATCC GCATGGAGGC GGCGCGCACC TGCTCACGTC TGCTTACCCC CTCCATCCAC 
CTGCTCAGCG GACATGGACA CGTGGTCAGT CAGACTGCTG TGCAGGTGGT GGCTGACGTT 
CTCAGCAAGC TACTTGTGGT TGGCATCACT GACCCAGACC CTGATATTCG ATACTGTGTG 
CTGGCCTCCC TGGACGAACG CTTTGATGCC CACCTGGCAC AAGCAGAGAA CCTCCAGGCT 
CTGTTTGTGG CGCTGAATGA TGAAGTGTTT GAGATCCGCG AACTGGCCAT CTGCACCATC 
GGTCGACTCA GCAGTATGAA CCCTGCCTTT GTCATGCCCT TCTGGCCCAA GATGCTCATT 
CAGATTCTGA CTGAGCTGGA ACATAGCGGT GTGGGCCGAA ACAAAGAGCA GAGTGCCCGC 
ATGCTGGGTC ACTTGGTTTC CAATGCTCCC AGACTTATTC GCCCATACAT GGAGCCCATT 
CTCAAGGCCC TCATTTTAAA GCTGAAAGAT CCCGATCCAA ACCCTGGAGT TGTCATCTGT 
GTTTTAGCTA CTATTGGAGA ACTTGCCCAG GTGAGTGGAC TGGAGATGAG GAAATGGATG 
GATGAGCTGT TCCCAATCAT TATGGACATG CTGCAGGACT CATCTTCATT AGCCAAGAGA 
CAGGTGGCAC TTTGGACTCT GGGCCAACAA GTAGCCAGCA CTGGGTATGT GGTGGAGCCT 
TACAGGAAGT ATCCATCTCT ACTGGAGGTG CTGCTCAACT TCCTGAAGAC AGAACAAAAC 
CAGGGGATCA GGAGAGAGGC CATTCGTGTT CTAGGTCTGC TAGGAGCTCT GGATCCCTAC 
AAACACAAAG TGAACATCGG AATGATCGAC CAGTCTCGAG ATGCTTCTGC CGTCAGCCTC 
TCCGAGTCCA AGTCCAGCCA GGATTCAGCT GACTACAGCA CCAGCGAGAT GCTGGTCAAC 
ATGGGCAACC TGCCGCTAGA CGAGTTTTAC CCAGCAGTCG CCATAGTGAC CCTGATGCGT 
ATCCTGCGTG ACCCGTCGCT GTCCAATCAC CACACCATGG TCGTTCAGGC CGTGACCTTC 
ATTTTCAAAT CTCTTGGCCT CAAATGTGTG CAGTTCCTGC CTCAGGTCAT GCCCACCTTT 
CTCAACGTCA TCAGAGTGTG TGATGCCAGC ATCAGAGAGT TTCTTTTCCA GCAAATGGGG 
ATGGTTGTGT GCTTCGTCAA AATCCACATC CGGCCCTACA TGGATGATAT CTTCACACTA 
ATCCGGGAAT ACTGGACTCC AAACAACCCT ATGCAGAACA CCATCATTTT GCTGATTGAG 
CAGATTGTTG TGGCTCTCGG TGGAGAATTC AAACTCTACC TTCCACAGCT CATCCCACAC 
ATGCTGCGAG TCTTCATGCA CGACATGAGT GTTGGACGCA ATGTCACCAT CAAGTTACTC 
ATGGCCATCC AACTGTTCGG AGCTAACCTG GATGACTATC TCCACCTGCT GCTGCCTCCT 
GTAGTGAAAC TGTTCGATGC CCCGGATGTC CCTCTGCAAG CCCGCAAGGT TGCTTTGGAG 
ACTTTAGATC GCCTGACTGA ATCGTTGGAC TTCACGGATT ATGCCTCCAG AATTATCCAC 
CCCATTGTTC GCACTCTTGA TGTTACTCCA GAGCTGAGGA ACTCCTCCAT GGACACACTG 
TCCTCGCTGG TCTTCCAGCT TGGTAAAAAG TATCAGATCT TCATTCCTAT GGTGAACAAA 
GTGATGTTGA AACACCGCAT CAATCACCAG CGCTATGATG TTCTCATCTG TCGTATCGTC 
AAGGGATATA CTTTGGCCGA AGAGGAAGAG GACCCTTTAA TATTTCAGCA CAGACAATTG 
CGTAGTAGTC AAAGCGACAC ACTGGTCAGT GGCCCAGTGG AATCTGGACC TATGAAGAAG 
CTGCACGTCA GCACAACTGC TCTTCAGAAG GCATGGGGTG CAGCCAGAAA GGTGTCCAAA 
GATGACTGGC TGGAGTGGTT GAGAAGACTT AGTGTGGTCT TGCTAAAGGA ATCCTCCTCA 
CCAGCCCTGC GCTCCTGCTG GTCTCTAGCA CAGACATATA TACCACTGGC TAGGGATCTT 
TTCAATGCTG CATTCTTATC GTGCTGGTCC GAGCTGAGTG AAGACCAGCA GGATGAGCTG 
ATCCGCAGTA TTGAGCTTGC TCTGACATCT CAGGATATTG CTGAGGTCAC ACAGACACTG 
CTCAACCTCG CAGAATTCAT GGAGCACAGC GATAAGGGCC CACTTCCTTT GAGAGATGAC 
AATGGGATAG TTCTGCTGGG GGAGAGAGCT GCTAAATGTC GGGCCTACGC CAAAGCCCTG 
CACTACAAGG AGCTGGAGTT TCAAAAGGGG GCTTCACCGC TCATTTTAGA ATCTCTGATC 
AGCATCAATA ATAAGCTCCA GCAGCCAGAG GCCGCATCAG GAGTGCTGGA GTATGCCATG 
AAACACTTTG GAGAATTGGA GATCCAGGCC ACCTGGTATG AGAAACTTCA TGAGTGGGAA 
GATGCTCTGG TTGCCTACGA TAAAAAAATC GACATGAACA AAGACGATCC AGAGCTTATT 
TTGGGTCGCA TGAGATGTCT GGAAGCACTA GGCGAATGGG GTCAGCTTCA CCAACAGTGC 
TGTGAGGAGT GGACTCTGGT CAGTGAAGAA ACTCAAGCCA AGATGGCCCG CATGGCAGCA 
GCTGCGGCGT GGGGACTGGG CCACTGGGAC AGTATGGAAG AGTATACCTG CATGATCCCG 
CGAGACACCC ACGATGGAGC CTTTTACCGG GCAGTGCTTG CTTTACATCA GGATCTGTTT 
TCATTGGCTC AGCAGTGTAT TGACAAGGCC AGAGATCTGC TGGATGCCGA GCTCACCGCC 
ATGGCTGGAG AGAGTTACAG TAGAGCATAC GGGGCTATGG TATCATGCCA GATGCTTTCG 
GAGTTAGAGG AGGTGATCCA GTATAAACTG GTTCCTGAGA GAAGGGACAT CATCAGGGAA 
ACATGGTGGG AGAGACTGCA GGGTTGTCAA CGCATTGTGG AAGACTGGCA GAGGATCTTA 
ATGGTGCGCT CTTTGGTCAT TAACCCACAT GAGGACATGA GGACGTGGTT GAAGTACGCC 
AGCCTCTGTG GAAAAAGTGG AAGACTGGCG CTGGCCCATA AGACTCTGGT TCTCCTGCTG 
GGTGTGGATC CCTCCAAACA GCTCGACCAC CCCTTGCCCA CCGCCCACCC GCATGTCACC 
TACGCTTACA TGAAGTACAT GTGGAAGAGC ACTCGCAAGA TCGATGCCTT CCAGCATATG 
CAGCACTTTG TGCAGGGAAT GCAACAGCAA GCACAACATG CCATCGCAGC TGAAGACCAG 
CAGCACAAGC TGGAGCTGCA CAAACTAATG GCACGGTGTT TTCTGAAGCT GGGGGAGTGG 
CAGCTCAGCC TTCAGGGCAT CAATGAGAGC ACCATCCCTA AAGTCTTGCA GTATTACAGT 
CACTCAACGG AGCATGATCG CAACTGGTAC AAGGCCTGGC ATGCTTGGGC AGTAATGAAC 
TTTGAGGCCG TGCTTCACTA CAAGCATCAG AACCAGGGCC GCGATGAGAA GAAGAAACTG 
CGACACGCAA GCGGCGCCAG CGCCAACAGC GAAGCCAGTA ACAGCGACAG CGAAGCCGAC 
AGCACCGAGC ACAGTCCCGT ACCCTCACCC GGACAGAAGA AAGTCAACGA AGATTTGTCC 
AAGACTCTCC TTCTTTACAC AGTGCCTGCA GTGCAGGGGT TTTTCAGATC CATCTCACTC 
TCACGTGGAA ATAACTTACA GGACACACTG AGAGTCCTGA CCTTATGGTT TGATTATGGT 
CACTGGCCTG AAGTGAATGA AGCTCTAGTC GAAGGTATCA AGACCATTCA GATAGACACT 
TGGTTACAGG TGATTCCTCA ACTGATCGCT CGGATTGATA CGCCACGTGC ACTGGTGGGA 
CGGCTCATTC ACCAGCTACT CACTGACATC GGTCGCTACC ACCCTCAGGC TTTGATTTAT 
CCTCTCACCG TGGCCTCCAA ATCCACAACC ACTGCCCGTC ACAACGCAGC CAACAAGATC 
TTGAAGAACA TGTGTGAGCA CTGCAACACG CTGGTCCAGC AAGCTATTAT GGTGAGCGAG 
GAGCTTATCA GGGTCGCTAT TCTCTGGCAT GAAATGTGGC ACGAGGGGCT GGAGGAAGCG 
TCACGTCTAT ATTTTGGAGA GAGGAACGTC AAGGGAATGT TTGCCGTTCT GGAGCCCCTC 
CATGCCATGA TGGAGAGAGG ACCACAAACC CTAAAAGAAA CATCATTTAA TCAGGCTTAT 
GGCCGAGATC TGATGGAGGC GCAGGACTGG TGTAGAAAGT ACATGCGATC GGGTAACGTC 
AAGGACCTGA CGCAGGCGTG GGATCTGTAC TACCATGTGT TCCGCAGGAT CTCCAAACAG 
CTGCCACAGC TCACTTCTCT AGAGCTTCAG TACGTCTCAC CTAAACTGCT GATGTGTCGT 
GATTTGGAGT TGGCTGTGCC TGGAACATAT GACCCCAATC AGTCAATCAT ACGCATCCAG 
TCCATTGCTC CCTCGCTGCA GGTCATCACC TCCAAACAGC GGCCACGCAA ACTCACCATC 
ATGGGCAGCA ATGGCCATGA GTTCATGTTC CTGCTGAAGG GTCATGAAGA TTTACGACAG 
GACGAGAGGG TCATGCAGCT TTTTGGCTTG GTGAACACTC TACTGGCTAA TGACCCAGCC 
TCCCTGCGCA AGAACCTGAG TATTCAGCGC TATGCTGTGA TCCCGCTTTC CACCAACTCT 
GGCCTGATTG GCTGGGTTCC TCACTGTGAC ACGCTGCACG CACTGATTCG AGACTACAGA 
GAGAAGAAGA AGATCCTGCT CAACATTGAA CATCGCATCA TGCTCAGGAT GGCCCCTGAC 
TATGACCATT TGACTCTAAT GGAGAAGGTG GAAGTGTTTG AGCATGCAGT GAACAATACT 
GCAGGAGATG ACCTGGCCAA ACTGCTTTGG CTGAAGAGTC CCAGTTCAGA GGTCTGGTTT 
GACAGGAGGA CCAACTACAC ACGCTCGCTA GCTGTTATGT CCATGGTTGG CTATATCTTA 
GGACTTGGAG ACAGGCATCC TTCTAATCTG ATGTTGGACA GGCTCAGTGG AAAGATACTG 
CATATCGATT TTGGAGACTG TTTTGAGGTT GCAATGACCA GAGAAAAATT CCCAGAGAAA 
ATTCCTTTTA GATTAACTCG CATGTTAACC AATGCCATGG AGGTGACGGG ACTGGATGGT 
AACTACCGTA TCACATGCCA CACAGTGATG GAGGTACTCC GGGAACACAG GGACAGTGTC 
ATGGCTGTGC TGGAGGCCTT TGTCTATGAC CCGTTACTCA ACTGGAGGCT TATGGACACA 
AACACTAAAG GAAATAAGCG ATCCCGGACC AGAACTGACT CCTATACGGC AGGACAGTCT 
GTCGAAGCCA TGGAGGGTAT TGACCTGGGA GAAACAACGC ACAAAAAGCC TGGAACTACC 
GTGCCTGAGT CCATTCACTC TTTCATTGGA GACGGCCTTG TGCAACCTGA AGCCTTGAAC 
AAGAAAGCCA TTCAGATCAT CAACCGAGTG CGGGACAAGT TAACTGGTCG AGATTTTTCC 
CATGATGAGA CTCTGGACGT ACCAACACAA GTGGAGCTGC TCATTAAGCA AGCTACGTCA 
CATGAAAACC TGTGCCAATG CTACATTGGA TGGTGCCCTT TTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070679.2
CDS	126..7673
Translation	MSGTATVLQQFVSGLKSRNEDTRAKAAKDLQHYVTTELRELSQDEATTFYDELNHHIFELVSSSDVNEKKGGILAIVSLIGVEGGNATRISRFANYLRNLLPSSDSVVMEMASKAMGHLSMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAVSAPTFFFQQVQPFFDNIFYAVWDSKQAIREGAVSALRACLILTTQRETKEMQKPQWYKQTFEEAEKGFDETLAKEKGMNKDDRVHGALLILNELVRISSMEGERMREEMEEITQQQLVHDKYCKELMGFSTKPRHITPFTSFQSVQPQQSNALLGLLGYSTPQGFLSFGAAPVPSKSTLVESRYCRELMEERFDQVCRWVLKYRTSKNPLIQMTILNLLPRLAAFQPHTFTDQYLQDTMGHLLGCLKKEKERTAAFQALGLLVVAVRADIQPYLSKILEIIKAALPPKDFAHKRQKTMQVDATVFTCISMLSRAMGPSIQQDVKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAYQLSSPSLTNIPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLLSGHGHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDEVFEIRELAICTIGRLSSMNPAFVMPFWPKMLIQILTELEHSGVGRNKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPNPGVVICVLATIGELAQVSGLEMRKWMDELFPIIMDMLQDSSSLAKRQVALWTLGQQVASTGYVVEPYRKYPSLLEVLLNFLKTEQNQGIRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSADYSTSEMLVNMGNLPLDEFYPAVAIVTLMRILRDPSLSNHHTMVVQAVTFIFKSLGLKCVQFLPQVMPTFLNVIRVCDASIREFLFQQMGMVVCFVKIHIRPYMDDIFTLIREYWTPNNPMQNTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDMSVGRNVTIKLLMAIQLFGANLDDYLHLLLPPVVKLFDAPDVPLQARKVALETLDRLTESLDFTDYASRIIHPIVRTLDVTPELRNSSMDTLSSLVFQLGKKYQIFIPMVNKVMLKHRINHQRYDVLICRIVKGYTLAEEEEDPLIFQHRQLRSSQSDTLVSGPVESGPMKKLHVSTTALQKAWGAARKVSKDDWLEWLRRLSVVLLKESSSPALRSCWSLAQTYIPLARDLFNAAFLSCWSELSEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGASPLILESLISINNKLQQPEAASGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKIDMNKDDPELILGRMRCLEALGEWGQLHQQCCEEWTLVSEETQAKMARMAAAAAWGLGHWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCQMLSELEEVIQYKLVPERRDIIRETWWERLQGCQRIVEDWQRILMVRSLVINPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSKQLDHPLPTAHPHVTYAYMKYMWKSTRKIDAFQHMQHFVQGMQQQAQHAIAAEDQQHKLELHKLMARCFLKLGEWQLSLQGINESTIPKVLQYYSHSTEHDRNWYKAWHAWAVMNFEAVLHYKHQNQGRDEKKKLRHASGASANSEASNSDSEADSTEHSPVPSPGQKKVNEDLSKTLLLYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPEVNEALVEGIKTIQIDTWLQVIPQLIARIDTPRALVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHCNTLVQQAIMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFAVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQDWCRKYMRSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQSIIRIQSIAPSLQVITSKQRPRKLTIMGSNGHEFMFLLKGHEDLRQDERVMQLFGLVNTLLANDPASLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMEKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHRDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYTAGQSVEAMEGIDLGETTHKKPGTTVPESIHSFIGDGLVQPEALNKKAIQIINRVRDKLTGRDFSHDETLDVPTQVELLIKQATSHENLCQCYIGWCPFW

Target ORF information:

RefSeq Version	NM_001077211.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mechanistic target of rapamycin kinase (mtor), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077211.2

ORF Insert Sequence:

ATGTCTGGCA CTGCCACAGT TCTGCAGCAG TTTGTGAGTG GCCTGAAGAG TCGCAATGAA 
GACACTCGTG CCAAAGCTGC CAAAGACCTT CAGCACTACG TCACGACAGA ACTAAGAGAG 
CTGAGCCAAG ATGAAGCCAC CACTTTCTAC GATGAACTGA ACCACCACAT TTTTGAGTTG 
GTTTCCAGCT CAGATGTGAA TGAGAAAAAA GGCGGTATCC TTGCCATAGT GAGCCTGATT 
GGAGTGGAGG GAGGAAATGC CACCCGTATC AGCCGCTTTG CCAACTATTT GCGGAACCTG 
CTTCCGTCCA GTGACTCAGT GGTCATGGAG ATGGCCTCTA AAGCCATGGG TCACCTGTCC 
ATGGCGGGAG ACACCTTCAC TGCAGAGTAT GTGGAGTTTG AGGTGAAGAG AGCTCTGGAG 
TGGCTGGGAG CAGACAGGAA TGAAGGCAGA CGACATGCAG CGGTGCTGGT GTTACGAGAA 
CTGGCTGTCA GCGCACCTAC ATTCTTTTTC CAGCAGGTGC AGCCATTCTT TGATAACATT 
TTTTATGCAG TGTGGGACTC CAAACAGGCC ATTCGTGAGG GCGCAGTATC TGCTCTCAGA 
GCTTGTTTGA TCCTTACCAC TCAGAGAGAG ACCAAAGAGA TGCAGAAACC ACAGTGGTAC 
AAGCAAACAT TTGAGGAAGC CGAGAAGGGT TTTGATGAGA CTTTGGCGAA AGAGAAAGGG 
ATGAATAAGG ATGATCGTGT ACATGGAGCT CTACTGATTC TCAATGAGCT AGTGCGAATA 
AGCAGTATGG AGGGAGAGCG TATGAGAGAG GAGATGGAGG AGATCACGCA GCAGCAGTTG 
GTTCATGATA AGTACTGTAA GGAGTTGATG GGTTTCAGCA CTAAGCCTCG TCACATCACG 
CCCTTCACCA GTTTTCAGTC CGTTCAGCCG CAGCAGTCTA ACGCTCTGCT GGGGCTGCTG 
GGTTACAGCA CACCTCAGGG CTTCTTAAGT TTTGGAGCTG CACCTGTTCC CTCGAAAAGC 
ACACTGGTGG AGAGCCGCTA CTGCAGAGAA CTGATGGAGG AGCGCTTTGA CCAGGTGTGC 
CGTTGGGTGC TGAAATACAG GACCAGTAAG AATCCTCTAA TCCAGATGAC AATCTTAAAC 
CTACTGCCTC GACTTGCTGC CTTCCAGCCT CACACTTTCA CTGATCAGTA TTTGCAGGAC 
ACTATGGGGC ACCTGCTGGG CTGCCTAAAA AAAGAAAAGG AGCGGACGGC AGCATTTCAA 
GCGCTGGGGC TACTGGTGGT GGCTGTGAGG GCTGATATTC AGCCATACCT AAGCAAGATC 
CTGGAGATTA TCAAAGCTGC ATTGCCACCG AAAGACTTTG CGCACAAGAG GCAGAAGACC 
ATGCAGGTGG ATGCTACTGT ATTTACTTGC ATAAGTATGC TGTCCAGAGC TATGGGGCCT 
AGCATCCAAC AGGACGTCAA AGAACTTCTG GAGCCCATGC TAGCTGTTGG TCTCAGCCCT 
GCTTTGACTG CAGTGCTTTA CGACCTGAGC AGGCAGATCC CACAGCTGAA GAAGGACATT 
CAGGATGGTC TGCTAAAGAT GCTTTCACTT GTGCTCATGC ACAAGCCTCT TCGTCACCCG 
GGTATGCCCA AGGGCCTGGC GTATCAGCTG TCATCGCCCA GCCTGACCAA CATCCCTGAG 
GCTAGCGACG TGGGCAGCAT AACTCTGGCA CTGAGAACTC TCGGCAGCTT TGAATTTGAA 
GGCCACTCCT TGACCCAGTT TGTGCGTCAC TGTGCGGACC ACTTTTTGAA CAGCGAGCAC 
AAGGAGATCC GCATGGAGGC GGCGCGCACC TGCTCACGTC TGCTTACCCC CTCCATCCAC 
CTGCTCAGCG GACATGGACA CGTGGTCAGT CAGACTGCTG TGCAGGTGGT GGCTGACGTT 
CTCAGCAAGC TACTTGTGGT TGGCATCACT GACCCAGACC CTGATATTCG ATACTGTGTG 
CTGGCCTCCC TGGACGAACG CTTTGATGCC CACCTGGCAC AAGCAGAGAA CCTCCAGGCT 
CTGTTTGTGG CGCTGAATGA TGAAGTGTTT GAGATCCGCG AACTGGCCAT CTGCACCATC 
GGTCGACTCA GCAGTATGAA CCCTGCCTTT GTCATGCCCT TCTGGCCCAA GATGCTCATT 
CAGATTCTGA CTGAGCTGGA ACATAGCGGT GTGGGCCGAA ACAAAGAGCA GAGTGCCCGC 
ATGCTGGGTC ACTTGGTTTC CAATGCTCCC AGACTTATTC GCCCATACAT GGAGCCCATT 
CTCAAGGCCC TCATTTTAAA GCTGAAAGAT CCCGATCCAA ACCCTGGAGT TGTCATCTGT 
GTTTTAGCTA CTATTGGAGA ACTTGCCCAG GTGAGTGGAC TGGAGATGAG GAAATGGATG 
GATGAGCTGT TCCCAATCAT TATGGACATG CTGCAGGACT CATCTTCATT AGCCAAGAGA 
CAGGTGGCAC TTTGGACTCT GGGCCAACAA GTAGCCAGCA CTGGGTATGT GGTGGAGCCT 
TACAGGAAGT ATCCATCTCT ACTGGAGGTG CTGCTCAACT TCCTGAAGAC AGAACAAAAC 
CAGGGGATCA GGAGAGAGGC CATTCGTGTT CTAGGTCTGC TAGGAGCTCT GGATCCCTAC 
AAACACAAAG TGAACATCGG AATGATCGAC CAGTCTCGAG ATGCTTCTGC CGTCAGCCTC 
TCCGAGTCCA AGTCCAGCCA GGATTCAGCT GACTACAGCA CCAGCGAGAT GCTGGTCAAC 
ATGGGCAACC TGCCGCTAGA CGAGTTTTAC CCAGCAGTCG CCATAGTGAC CCTGATGCGT 
ATCCTGCGTG ACCCGTCGCT GTCCAATCAC CACACCATGG TCGTTCAGGC CGTGACCTTC 
ATTTTCAAAT CTCTTGGCCT CAAATGTGTG CAGTTCCTGC CTCAGGTCAT GCCCACCTTT 
CTCAACGTCA TCAGAGTGTG TGATGCCAGC ATCAGAGAGT TTCTTTTCCA GCAAATGGGG 
ATGGTTGTGT GCTTCGTCAA AATCCACATC CGGCCCTACA TGGATGATAT CTTCACACTA 
ATCCGGGAAT ACTGGACTCC AAACAACCCT ATGCAGAACA CCATCATTTT GCTGATTGAG 
CAGATTGTTG TGGCTCTCGG TGGAGAATTC AAACTCTACC TTCCACAGCT CATCCCACAC 
ATGCTGCGAG TCTTCATGCA CGACATGAGT GTTGGACGCA ATGTCACCAT CAAGTTACTC 
ATGGCCATCC AACTGTTCGG AGCTAACCTG GATGACTATC TCCACCTGCT GCTGCCTCCT 
GTAGTGAAAC TGTTCGATGC CCCGGATGTC CCTCTGCAAG CCCGCAAGGT TGCTTTGGAG 
ACTTTAGATC GCCTGACTGA ATCGTTGGAC TTCACGGATT ATGCCTCCAG AATTATCCAC 
CCCATTGTTC GCACTCTTGA TGTTACTCCA GAGCTGAGGA ACTCCTCCAT GGACACACTG 
TCCTCGCTGG TCTTCCAGCT TGGTAAAAAG TATCAGATCT TCATTCCTAT GGTGAACAAA 
GTGATGTTGA AACACCGCAT CAATCACCAG CGCTATGATG TTCTCATCTG TCGTATCGTC 
AAGGGATATA CTTTGGCCGA AGAGGAAGAG GACCCTTTAA TATTTCAGCA CAGACAATTG 
CGTAGTAGTC AAAGCGACAC ACTGGTCAGT GGCCCAGTGG AATCTGGACC TATGAAGAAG 
CTGCACGTCA GCACAACTGC TCTTCAGAAG GCATGGGGTG CAGCCAGAAA GGTGTCCAAA 
GATGACTGGC TGGAGTGGTT GAGAAGACTT AGTGTGGTCT TGCTAAAGGA ATCCTCCTCA 
CCAGCCCTGC GCTCCTGCTG GTCTCTAGCA CAGACATATA TACCACTGGC TAGGGATCTT 
TTCAATGCTG CATTCTTATC GTGCTGGTCC GAGCTGAGTG AAGACCAGCA GGATGAGCTG 
ATCCGCAGTA TTGAGCTTGC TCTGACATCT CAGGATATTG CTGAGGTCAC ACAGACACTG 
CTCAACCTCG CAGAATTCAT GGAGCACAGC GATAAGGGCC CACTTCCTTT GAGAGATGAC 
AATGGGATAG TTCTGCTGGG GGAGAGAGCT GCTAAATGTC GGGCCTACGC CAAAGCCCTG 
CACTACAAGG AGCTGGAGTT TCAAAAGGGG GCTTCACCGC TCATTTTAGA ATCTCTGATC 
AGCATCAATA ATAAGCTCCA GCAGCCAGAG GCCGCATCAG GAGTGCTGGA GTATGCCATG 
AAACACTTTG GAGAATTGGA GATCCAGGCC ACCTGGTATG AGAAACTTCA TGAGTGGGAA 
GATGCTCTGG TTGCCTACGA TAAAAAAATC GACATGAACA AAGACGATCC AGAGCTTATT 
TTGGGTCGCA TGAGATGTCT GGAAGCACTA GGCGAATGGG GTCAGCTTCA CCAACAGTGC 
TGTGAGGAGT GGACTCTGGT CAGTGAAGAA ACTCAAGCCA AGATGGCCCG CATGGCAGCA 
GCTGCGGCGT GGGGACTGGG CCACTGGGAC AGTATGGAAG AGTATACCTG CATGATCCCG 
CGAGACACCC ACGATGGAGC CTTTTACCGG GCAGTGCTTG CTTTACATCA GGATCTGTTT 
TCATTGGCTC AGCAGTGTAT TGACAAGGCC AGAGATCTGC TGGATGCCGA GCTCACCGCC 
ATGGCTGGAG AGAGTTACAG TAGAGCATAC GGGGCTATGG TATCATGCCA GATGCTTTCG 
GAGTTAGAGG AGGTGATCCA GTATAAACTG GTTCCTGAGA GAAGGGACAT CATCAGGGAA 
ACATGGTGGG AGAGACTGCA GGGTTGTCAA CGCATTGTGG AAGACTGGCA GAGGATCTTA 
ATGGTGCGCT CTTTGGTCAT TAACCCACAT GAGGACATGA GGACGTGGTT GAAGTACGCC 
AGCCTCTGTG GAAAAAGTGG AAGACTGGCG CTGGCCCATA AGACTCTGGT TCTCCTGCTG 
GGTGTGGATC CCTCCAAACA GCTCGACCAC CCCTTGCCCA CCGCCCACCC GCATGTCACC 
TACGCTTACA TGAAGTACAT GTGGAAGAGC ACTCGCAAGA TCGATGCCTT CCAGCATATG 
CAGCACTTTG TGCAGGGAAT GCAACAGCAA GCACAACATG CCATCGCAGC TGAAGACCAG 
CAGCACAAGC TGGAGCTGCA CAAACTAATG GCACGGTGTT TTCTGAAGCT GGGGGAGTGG 
CAGCTCAGCC TTCAGGGCAT CAATGAGAGC ACCATCCCTA AAGTCTTGCA GTATTACAGT 
CACTCAACGG AGCATGATCG CAACTGGTAC AAGGCCTGGC ATGCTTGGGC AGTAATGAAC 
TTTGAGGCCG TGCTTCACTA CAAGCATCAG AACCAGGGCC GCGATGAGAA GAAGAAACTG 
CGACACGCAA GCGGCGCCAG CGCCAACAGC GAAGCCAGTA ACAGCGACAG CGAAGCCGAC 
AGCACCGAGC ACAGTCCCGT ACCCTCACCC GGACAGAAGA AAGTCAACGA AGATTTGTCC 
AAGACTCTCC TTCTTTACAC AGTGCCTGCA GTGCAGGGGT TTTTCAGATC CATCTCACTC 
TCACGTGGAA ATAACTTACA GGACACACTG AGAGTCCTGA CCTTATGGTT TGATTATGGT 
CACTGGCCTG AAGTGAATGA AGCTCTAGTC GAAGGTATCA AGACCATTCA GATAGACACT 
TGGTTACAGG TGATTCCTCA ACTGATCGCT CGGATTGATA CGCCACGTGC ACTGGTGGGA 
CGGCTCATTC ACCAGCTACT CACTGACATC GGTCGCTACC ACCCTCAGGC TTTGATTTAT 
CCTCTCACCG TGGCCTCCAA ATCCACAACC ACTGCCCGTC ACAACGCAGC CAACAAGATC 
TTGAAGAACA TGTGTGAGCA CTGCAACACG CTGGTCCAGC AAGCTATTAT GGTGAGCGAG 
GAGCTTATCA GGGTCGCTAT TCTCTGGCAT GAAATGTGGC ACGAGGGGCT GGAGGAAGCG 
TCACGTCTAT ATTTTGGAGA GAGGAACGTC AAGGGAATGT TTGCCGTTCT GGAGCCCCTC 
CATGCCATGA TGGAGAGAGG ACCACAAACC CTAAAAGAAA CATCATTTAA TCAGGCTTAT 
GGCCGAGATC TGATGGAGGC GCAGGACTGG TGTAGAAAGT ACATGCGATC GGGTAACGTC 
AAGGACCTGA CGCAGGCGTG GGATCTGTAC TACCATGTGT TCCGCAGGAT CTCCAAACAG 
CTGCCACAGC TCACTTCTCT AGAGCTTCAG TACGTCTCAC CTAAACTGCT GATGTGTCGT 
GATTTGGAGT TGGCTGTGCC TGGAACATAT GACCCCAATC AGTCAATCAT ACGCATCCAG 
TCCATTGCTC CCTCGCTGCA GGTCATCACC TCCAAACAGC GGCCACGCAA ACTCACCATC 
ATGGGCAGCA ATGGCCATGA GTTCATGTTC CTGCTGAAGG GTCATGAAGA TTTACGACAG 
GACGAGAGGG TCATGCAGCT TTTTGGCTTG GTGAACACTC TACTGGCTAA TGACCCAGCC 
TCCCTGCGCA AGAACCTGAG TATTCAGCGC TATGCTGTGA TCCCGCTTTC CACCAACTCT 
GGCCTGATTG GCTGGGTTCC TCACTGTGAC ACGCTGCACG CACTGATTCG AGACTACAGA 
GAGAAGAAGA AGATCCTGCT CAACATTGAA CATCGCATCA TGCTCAGGAT GGCCCCTGAC 
TATGACCATT TGACTCTAAT GGAGAAGGTG GAAGTGTTTG AGCATGCAGT GAACAATACT 
GCAGGAGATG ACCTGGCCAA ACTGCTTTGG CTGAAGAGTC CCAGTTCAGA GGTCTGGTTT 
GACAGGAGGA CCAACTACAC ACGCTCGCTA GCTGTTATGT CCATGGTTGG CTATATCTTA 
GGACTTGGAG ACAGGCATCC TTCTAATCTG ATGTTGGACA GGCTCAGTGG AAAGATACTG 
CATATCGATT TTGGAGACTG TTTTGAGGTT GCAATGACCA GAGAAAAATT CCCAGAGAAA 
ATTCCTTTTA GATTAACTCG CATGTTAACC AATGCCATGG AGGTGACGGG ACTGGATGGT 
AACTACCGTA TCACATGCCA CACAGTGATG GAGGTACTCC GGGAACACAG GGACAGTGTC 
ATGGCTGTGC TGGAGGCCTT TGTCTATGAC CCGTTACTCA ACTGGAGGCT TATGGACACA 
AACACTAAAG GAAATAAGCG ATCCCGGACC AGAACTGACT CCTATACGGC AGGACAGTCT 
GTCGAAGCCA TGGAGGGTAT TGACCTGGGA GAAACAACGC ACAAAAAGCC TGGAACTACC 
GTGCCTGAGT CCATTCACTC TTTCATTGGA GACGGCCTTG TGCAACCTGA AGCCTTGAAC 
AAGAAAGCCA TTCAGATCAT CAACCGAGTG CGGGACAAGT TAACTGGTCG AGATTTTTCC 
CATGATGAGA CTCTGGACGT ACCAACACAA GTGGAGCTGC TCATTAAGCA AGCTACGTCA 
CATGAAAACC TGTGCCAATG CTACATTGGA TGGTGCCCTT TTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Gu Y,Gao L,Han Q,Li A,Yu H,Liu D,Pang Q

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Environmental pollution (Barking, Essex : 1987)242(Pt B)1939-1949(2018 Nov)
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Inhibited Carnitine Synthesis Causes Systemic Alteration of Nutrient Metabolism in Zebrafish.
Frontiers in physiology9509(2018)
Li JM,Li LY,Qin X,Degrace P,Demizieux L,Limbu SM,Wang X,Zhang ML,Li DL,Du ZY

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mtor cDNA ORF clone, Danio rerio(zebrafish)

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