mt2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mt2
Entrez Gene ID	100174951
Full Name	metallothionein 2
Synonyms	MT,MT-2,mt2b,wu:fc77e11,wu:fj10c11,zgc:112061
General protein information	Preferred Names metallothionein 2 Names metallothionein-2 metallothionein IIA metallothionein IIB
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001131053.3	NP_001124525.2	metallothionein-2
NM_001131053.2	NP_001124525.2	metallothionein-2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative transcriptional analysis of methylparaben and propylparaben in zebrafish.
Bereketoglu C, Pradhan A
The Science of the total environment671129-139(2019 Jun)

Zebrafish behavioral phenomics applied for phenotyping aquatic neurotoxicity induced by lead contaminants of environmentally relevant level.
Li X, Kong H, Ji X, Gao Y, Jin M
Chemosphere224445-454(2019 Jun)

Negative effects of acute cadmium on stress defense, immunity, and metal homeostasis in liver of zebrafish: The protective role of environmental zinc dpre-exposure.
Wang CC, Si LF, Guo SN, Zheng JL
Chemosphere22291-97(2019 May)

Assessment of environmentally contaminated sediment using a contact assay with early life stage zebrafish (Danio rerio).
Boulanger E, Barst BD, Alloy MM, Blais S, Houde M, Head JA
The Science of the total environment659950-962(2019 Apr)

Protective Effects of
Gombeau K, de Oliveira RB, Sarrazin SLF, Mourão RHV, Bourdineaud JP
Toxicological research35(1)25-35(2019 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38955	NM_001131053.3 Latest version!	Danio rerio metallothionein 2 (mt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
ODa38955	NM_001131053.2	Danio rerio metallothionein 2 (mt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mt2 ( NM_001131053.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001124525.2 Danio rerio metallothionein 2 (mt2), mRNA.

Price & Availability↑

CloneID	ODa38955
Clone ID Related Accession (Same CDS sequence)	NM_001131053.3 , NM_001131053.2
Accession Version	NM_001131053.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576771200000
Organism	Danio rerio(zebrafish)
Product	metallothionein-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX908765.9. On Feb 2, 2019 this sequence version replaced NM_001131053.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GDQQ01036852.1, EH508165.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGACCCCT GCGAATGTGC CAAGACTGGA ACTTGCAACT GTGGTGCTAC CTGCAAGTGC 
ACTAATTGCC AGTGTACTAC CTGCAAGAAG AGTTGTTGTT CTTGCTGCCC ATCTGGTTGC 
AGCAAGTGTG CCTCTGGCTG CGTCTGCAAA GGCAATTCCT GTGGCTCCAG CTGCTGTCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124525.2
CDS	76..258
Translation	MDPCECAKTGTCNCGATCKCTNCQCTTCKKSCCSCCPSGCSKCASGCVCKGNSCGSSCCQ

Target ORF information:

RefSeq Version	NM_001131053.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio metallothionein 2 (mt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001131053.3

ORF Insert Sequence:

ATGGACCCCT GCGAATGTGC CAAGACTGGA ACTTGCAACT GTGGTGCTAC CTGCAAGTGC 
ACTAATTGCC AGTGTACTAC CTGCAAGAAG AGTTGTTGTT CTTGCTGCCC ATCTGGTTGC 
AGCAAGTGTG CCTCTGGCTG CGTCTGCAAA GGCAATTCCT GTGGCTCCAG CTGCTGTCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mt2 ( NM_001131053.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001124525.2 Danio rerio metallothionein 2 (mt2), mRNA.

Price & Availability↑

CloneID	ODa38955
Clone ID Related Accession (Same CDS sequence)	NM_001131053.3 , NM_001131053.2
Accession Version	NM_001131053.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542384000000
Organism	Danio rerio(zebrafish)
Product	metallothionein-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX908765.9 and BC152694.1. On Jun 3, 2009 this sequence version replaced NM_001131053.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049475.1, EH440239.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGACCCCT GCGAATGTGC CAAGACTGGA ACTTGCAACT GTGGTGCTAC CTGCAAGTGC 
ACTAATTGCC AGTGTACTAC CTGCAAGAAG AGTTGTTGTT CTTGCTGCCC ATCTGGTTGC 
AGCAAGTGTG CCTCTGGCTG CGTCTGCAAA GGCAATTCCT GTGGCTCCAG CTGCTGTCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124525.2
CDS	76..258
Translation	MDPCECAKTGTCNCGATCKCTNCQCTTCKKSCCSCCPSGCSKCASGCVCKGNSCGSSCCQ

Target ORF information:

RefSeq Version	NM_001131053.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio metallothionein 2 (mt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001131053.2

ORF Insert Sequence:

ATGGACCCCT GCGAATGTGC CAAGACTGGA ACTTGCAACT GTGGTGCTAC CTGCAAGTGC 
ACTAATTGCC AGTGTACTAC CTGCAAGAAG AGTTGTTGTT CTTGCTGCCC ATCTGGTTGC 
AGCAAGTGTG CCTCTGGCTG CGTCTGCAAA GGCAATTCCT GTGGCTCCAG CTGCTGTCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative transcriptional analysis of methylparaben and propylparaben in zebrafish.
The Science of the total environment671129-139(2019 Jun)
Bereketoglu C,Pradhan A

Zebrafish behavioral phenomics applied for phenotyping aquatic neurotoxicity induced by lead contaminants of environmentally relevant level.
Chemosphere224445-454(2019 Jun)
Li X,Kong H,Ji X,Gao Y,Jin M

Negative effects of acute cadmium on stress defense, immunity, and metal homeostasis in liver of zebrafish: The protective role of environmental zinc dpre-exposure.
Chemosphere22291-97(2019 May)
Wang CC,Si LF,Guo SN,Zheng JL

Assessment of environmentally contaminated sediment using a contact assay with early life stage zebrafish (Danio rerio).
The Science of the total environment659950-962(2019 Apr)
Boulanger E,Barst BD,Alloy MM,Blais S,Houde M,Head JA

Protective Effects of
Toxicological research35(1)25-35(2019 Jan)
Gombeau K,de Oliveira RB,Sarrazin SLF,Mourão RHV,Bourdineaud JP