LOC552814 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC552814
Entrez Gene ID	552814
Full Name	activating signal cointegrator 1 complex subunit 3
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_026443881.1	XP_026299666.1	activating signal cointegrator 1 complex subunit 3 isoform X1
XM_026443884.1	XP_026299669.1	activating signal cointegrator 1 complex subunit 3 isoform X2
XM_026443880.1	XP_026299665.1	activating signal cointegrator 1 complex subunit 3 isoform X1
XM_026443882.1	XP_026299667.1	activating signal cointegrator 1 complex subunit 3 isoform X1
XM_026443885.1	XP_026299670.1	activating signal cointegrator 1 complex subunit 3 isoform X2
XM_006571832.2	XP_006571895.2	activating signal cointegrator 1 complex subunit 3
XM_026443883.1	XP_026299668.1	activating signal cointegrator 1 complex subunit 3 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC552814 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC552814 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHd17758	XM_006571832.2 Latest version!	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHd25552	XM_026443881.1 Latest version!	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHd25552	XM_026443880.1 Latest version!	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHd25552	XM_026443882.1 Latest version!	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHd25553	XM_026443885.1 Latest version!	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHd25553	XM_026443883.1 Latest version!	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHd25553	XM_026443884.1 Latest version!	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC552814 ( XM_006571832.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006571895.2 Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), mRNA.

Price & Availability↑

CloneID	OHd17758
Clone ID Related Accession (Same CDS sequence)	XM_006571832.2
Accession Version	XM_006571832.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463932800000
Organism	Apis mellifera(honey bee)
Product	activating signal cointegrator 1 complex subunit 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003378199.1) and transcript sequence (HP497081.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006571832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 66 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTGTGG GCAACATTAA TCAAAGTACA GATAATTTCT CAATGAAATA TAAAAATGAA 
GCTACAAAAT TATTTAAAAC AGAATTTGAC AAGACAGCAT ATAAGGAAAA CTTGAAATCA 
TATCATACAA AGAATTTTGC AGATATATCT TTAGAAAAAT TTGAGAAGAT CATAATTACT 
AAATTAGGTT CTAAAAATTC AGAGAACGAA TTAGTTGATT TATTAGGTTG TGAATCTATA 
GAATTTATTC AATATATTGT TCAACATCAG AAAAGTATTA TAACTTTAAG TTCTACTCCA 
AAAATACAAA AAAAATGTAA TATTCAAGGA CCTGTGATCA GTGGCCAAGT AACAGTTCAA 
TCTGAGAAAG AAAAACAATT ACAAAAACAA ATACGGAAAC ACGAAAAAAA ATTAAATAAA 
ACGAGTAAAC GAGAAGGAAA GGGAGAGTTG GAAGAAAATG GCTTTGAACC ATTAGAATTG 
ACTTTAAAAA AAAATGAAGC ATTAATAGAA ATGCACACAC CTATATTTAA AAAAAGTATT 
AATACAGATC GAGAAAAATT TCCTTTTGTG TTTGATGCTA ATATGAACAG TAGAAATACA 
ACTGGTTATG TATCAAAACA AAAAATAATG TTACCGCAAA ATGTTATAAG GAAAGATACA 
GAAACATTTG AAGAAATTTT TATACCTATT CCAGAATCTC AACCACTTGA TGTAGATTAT 
AAACCTATTA TGGTATCTTC ATTGGATGAT ATTGGACAAA TGGCTTTTAA TGGCATTGAA 
TCATTAAATC GAATACAAAG TATTGTGTTC GATGTAGCAT ATCATACTAA TGAGAATTTA 
TTAATTTGTG CTCCAACAGG AGCTGGAAAG ACTAATGTTG CTATGTTAAC TATAGTACAT 
CAATTAAAGC AACATATTGA ATATGGTCAA TTAATGAAAA ATCAGTTCAA AATAATTTAT 
GTAACTCCAA TGAAAGCTTT AGCAGCTGAA ATGACAGCAA ATTTTAGCAA AAAACTTCAT 
TGTCTTGGTA TATCTGTTCG CGAGTTAACT GGAGATATGC AATTAACCAA ATCGGAAATT 
CAACAAACAC AGATGATTGT TACCACACCA GAAAAGTGGG ATGTTGTTAC TAGAAAAGGT 
ACTGGAGATA TTTCTCTCAC AAGTATTGTA AAACTGTTAA TTATTGATGA AGTACATTTG 
TTACATGGTG ATAGAGGACC TGTGGTTGAA GCCTTAGTTG CTCGAACTTT GAGACAAGTA 
GAATCTTCTC AAAGTATGAT CAGAATTGTT GGTCTTTCTG CAACATTGCC AAATTATGTA 
GATGTTGCAA GATTTTTAAG AGTGAATTCA AACATAGGAC TTTTTTATTT TGATCATCGA 
TTTCGACCAG TACCACTTAG TCAAGCGTTT ATTGGTGTAA AAGCATCATC ATCATTACAA 
GAAATGAATT ACATGGATAA TGTATGTTAC AATAATGTTA TAGATATGGT ACGTCAAGGA 
TATCAAGTAA TGGTATTTGT ACATGCACGA AATGCTACTG TTAGAATGGC AAATACACTA 
AAAGATTTAG CAATAAAACA TGGTACACTT AAATTATTTA TACCTGAAGG TCAAACAAAA 
TTTATAAATA AAGCTTTTGC TAAATCACGT ACGAAGTATT TAAGTGAATT ATTTAATAAT 
GGATTATCAG TACATCATGC TGGTTTATTA CGTTCAGAGA GAAATTTAAT TGAAAAATAT 
TTTGCAGATG GTTTAATTAA AGTATTAGTA TGTACGTCAA CATTAGCTTG GGGTGTAAAT 
CTACCTGCCC ATGCTGTTAT TATAAGAGGA ACAGAAATAT ATGACGCAAA ACATGGTTCT 
TATATTGACT TAGATATATT AGATGTTTTA CAAATTTTTG GTCGTGCTGG TAGACCACAA 
TTTGATGTAT CTGGTCATGC TGTTATTATT ACTTCCCATA ATAAATTATA CCATTACTTA 
TCATTATTAA CAAATCAAAT ACCTATAGAA AGCAATTTCA TTAAATATTT AGCTGATAAT 
TTAAATGCTG AAATTGCATT GGGTACAATA TCGAATGTAG AAGAAGCAAT AAAATGGTTA 
AGTTATACTT ATTTATTCGT TCGAATGAAA TTAAATTTTC ATTTTTATGG AATTCCTTAT 
CGAGTTATTT TGGAAGATCA AAATTTAGAA CAAAAAAGAA GAGAACTAAT TGATCAAGCA 
GCTAAAGCAT TAGATGAAGC TAAAATGATT CGATATAATA TAAATACTGG AGATCTGAGC 
ACAACAAATT TAGGACGAAT TGCGAGCCAT TTTTATCTTA AATATGACAC TATTAAAATT 
TTTAATACAC TTCTAAAACC AATTATGACT GAAGCAGAAA TATTTTCTAT GATATCATAT 
TCTCAAGAAT TTGAACAATT AAAAGTGCGA GATGATGAAA TGGAGGAATT ACAATATTTA 
TTTGAAGAAT ATTGTAATAT TACAGTCCAA GGTGGTGTTG AAAATATATA TGGTAAAGTT 
AATATATTAT TACAAACGTA TTTGTCACAT GGTAGAGTTA ATGCGTTTTC ATTAATATCT 
GATCAAGCAT ATATTGTACA AAATGCAGGA CGGATATTTC GAGCGTTGTT TGAAATTATG 
TTAGGACAAA ATAATGCAAT AATGGCTGGT CGTTTATTAG AAATAGCAAA AGTACTCGAA 
ATACAACAAT GGAGTGATAG AAGTCCTCTA TGTCAATTTT CTTGTTTATC TTTAGAAATT 
ATTGACAAGA TAGAACAATA TGATTTAACA GTTGAAAGAT TAAATAATAT GAATGTTAAA 
GAAATTGGAG ATATTTTGCG TAATCAGAAA GAAGCAATAT TGGTGAAAAA ATGTTGCAAA 
GAATTACCTG CATTAGAAAT GGAATCAAAT TTACAACCTA TTACTCGTAC AGTTTTAAGA 
ATACATTTAA AAATATATCC TCAATTTCAT TGGAATGATA ATGTTCATGG TAAAACTTCA 
GAACCATTCT GGATATGGAT AGAAGATCCA GATAATAATT TTATTTATCA TCATGAATAT 
TTTACTATGA CAAGAAAAAT GGTTTACAAT AATCTTGCAC AAGAACTTGT GATGACAATA 
CCATTGCATG AACCATTGCC TACTCAATAC ATAGTAAGAG CAATAAGTGA TCGCTGGTTG 
GGTTCTGAAT ATATGTTACC TTTAACATTT CATGATTTAA TTTTACCTGA AGTATATCCA 
CCATATACTG ATTTGTTAGA AGTACAACCT CTGCCAATAA AAGCATTGAA AGAACCATTA 
TTTGAAAAAC TTTATAAATT TTCTCATTTT AATCCAATAC AGACACAAAT TTTTCATTGT 
TTGTATCATA CAGACAATAA CGTCCTATTA GGAGCTCCAA CTGGTTCAGG AAAAACAATT 
GCTGCGGAAA TTGCAATGTT TAGAGTATTT AAACAATATC CAACGCAAAA AATTGTATAT 
ATTGCACCAT TGAAAGCTTT AGTAAGAGAA CGAATGAAAG ATTGGAAAAT TAGATTTGAA 
GAACAATTAG GAAAAAAAGT AATAGAATTA ACTGGAGATG TATCACCAGA TATTAAAGTT 
ATAGCAAATG CAAATATTAT TATAACTACT CCAGAAAAAT GGGATGGTAT TAGTAGAAGT 
TGGCAAACAA GACTTTATGT CAAAAATGTT GCGCTTATTA TTATAGATGA AATACATTTA 
TTAGGGGAAG ATCGTGGACC AGTATTAGAA GTTATTATTT CAAGAACTAA TTTTATTTCT 
AGTCATACTT TAAAAAAATT AAGAGTTATT GGACTTTCTA CAGCATTAGC TAATGCAGTA 
GATTTAGCTA ATTGGCTTGG TATTAAAGAG ATGGGTCTTT ATAATTTTCG TCCATCTGTA 
CGACCTGTAC CATTAGATAT TCATATAACT GGATTCCCAG GGAAAAATTA TTGTCCTCGA 
ATGGCAACAA TGAATAGACC AACTTTTCAA GCAATTAAAC AACATGCTCC AACTAGTCCG 
GCTTTAGTAT TTGTTTCATC ACGTAGACAA ACACGTTTAA CTGCATTAGA TTTGATTGCA 
TATCTTGCAG GAGAAGATAA TCCTAAGCAA TGGTTACATA TGAGAGAAGA AGAAATGAAT 
AACATTCTAA GTCATATACG AGATTCAAAT TTGAAACTTA CACTTGCTTT TGGAATTGGA 
CTTCATCATG CAGGTTTTCA GGAAAGAGAT AGAAAAATTG TCGAAGAATT ATTTGTTAAC 
AATAAAATTC AGGTATTGAT TACAACAGCT ACTTTAGCTT GGGGAGTTAA TTTCCCTGCA 
CACTTAGTTG TAATAAAAGG AACAGAATAT TATGATGGCA AACAACACCG TTATGTAGAC 
ATGCCAATCA CAGATGTACT ACAAATGATG GGTCGTGCAG GAAGACCCCA GTTTGATAAT 
TCAGGAGTGG CAGTAGTTTT AGTACACGAT TTAAAAAAAA ATTATTATAA GAAATTTTTA 
CATCATCCAT TTCCAGTAGA ATCAAGTTTA TTAGCTGTTC TGCCAGATCA TATAAATGCT 
GAAATTGTAG CTAATACAAT TAAAAATAAA CAAGAATTAT TAGATTATTT AACATGGACA 
TATTTTTTTA GAAGGTTAAT GAAAAATCCT AGATATTACA ATTTAAGTAT TTTAGAACCT 
TATGCTATTA ATCAATATCT CTCTTCTTTG GTTGATAATA CACTAAAAGT ATTAATAGAT 
TCATATTGTG TGGCATTTGA TGAGGAAGAA CAAATTTTAT ATCCACTTTC AATGGGTAAA 
ATAGCATCAT TTTATTACTT ATCGCATCAT ACTATGTTAA TGTTTGTACA ATCATTACAA 
GAATCTCTTA CATTGGAGCA ATGTTTACAT ATTTTATGTA ATTCTTATGA ATATAATGAA 
TTACCAGTAA GACACAATGA AGAATTATTG AATGAGGAAT TAAGTAAAAT GTGCCGTTAT 
CAAATAGATA ATTACTCATA TAATTCTCCT CATACCAAAG CATTTTTATT ACTTCAAGCA 
CATTTCTCAA GACTTCCCTT ACCATGTGTA GATTATTTTA CTGATTTAAA ATCAGTTCTT 
GATCAGGCAA TTAGGATTAT ACAAGCAATG ATAGATGCAG TTGCAGATCA TGGATGGTTA 
GCAAATACAA TTATGATAAT GAACATACTT CAAATGATTA TTCAAGCTCG ATGGATTGAT 
GAATCAGCAA TTATTACATT GCCATGTGTA AATTCAGAAC ATTTAGAATT ATTTTCAACA 
TTATCGCTTA CTCTTCCTGA ATTATGTTTC AATATGCACA ACAAAGATAT TAAAATACTT 
AGAAAAGTAC TTAATAAAAG TTTTTCGCAA GAACAAATAT ATCAGATATA TCAAGTAATA 
AAAGAAATGC CAATGCTCTG TATTAAGTTA AGCTTAGAAA GTTATGATGA AGATAATGAT 
AATAATAAAC AAAAAAATCA AATATTTATA CCTCTAAAAT CTGATAATTT GGATTATATA 
AATATTCACA AGGATCAAGA TTACATATTA AATATTATAA TGAAAAGAAA AAATAAATCT 
AATAGTTTAA AAGCACACAG TCCTGTGTTT CAAAAAGGAA AGGATGAAGG TTGGTTTTTA 
ATTTTAGGTA ATGTTTCTGA TAAAGAATTA TTAGTTCTAT ACGAGCATCT GCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006571895.2
CDS	115..5811
Translation	MSVGNINQSTDNFSMKYKNEATKLFKTEFDKTAYKENLKSYHTKNFADISLEKFEKIIITKLGSKNSENELVDLLGCESIEFIQYIVQHQKSIITLSSTPKIQKKCNIQGPVISGQVTVQSEKEKQLQKQIRKHEKKLNKTSKREGKGELEENGFEPLELTLKKNEALIEMHTPIFKKSINTDREKFPFVFDANMNSRNTTGYVSKQKIMLPQNVIRKDTETFEEIFIPIPESQPLDVDYKPIMVSSLDDIGQMAFNGIESLNRIQSIVFDVAYHTNENLLICAPTGAGKTNVAMLTIVHQLKQHIEYGQLMKNQFKIIYVTPMKALAAEMTANFSKKLHCLGISVRELTGDMQLTKSEIQQTQMIVTTPEKWDVVTRKGTGDISLTSIVKLLIIDEVHLLHGDRGPVVEALVARTLRQVESSQSMIRIVGLSATLPNYVDVARFLRVNSNIGLFYFDHRFRPVPLSQAFIGVKASSSLQEMNYMDNVCYNNVIDMVRQGYQVMVFVHARNATVRMANTLKDLAIKHGTLKLFIPEGQTKFINKAFAKSRTKYLSELFNNGLSVHHAGLLRSERNLIEKYFADGLIKVLVCTSTLAWGVNLPAHAVIIRGTEIYDAKHGSYIDLDILDVLQIFGRAGRPQFDVSGHAVIITSHNKLYHYLSLLTNQIPIESNFIKYLADNLNAEIALGTISNVEEAIKWLSYTYLFVRMKLNFHFYGIPYRVILEDQNLEQKRRELIDQAAKALDEAKMIRYNINTGDLSTTNLGRIASHFYLKYDTIKIFNTLLKPIMTEAEIFSMISYSQEFEQLKVRDDEMEELQYLFEEYCNITVQGGVENIYGKVNILLQTYLSHGRVNAFSLISDQAYIVQNAGRIFRALFEIMLGQNNAIMAGRLLEIAKVLEIQQWSDRSPLCQFSCLSLEIIDKIEQYDLTVERLNNMNVKEIGDILRNQKEAILVKKCCKELPALEMESNLQPITRTVLRIHLKIYPQFHWNDNVHGKTSEPFWIWIEDPDNNFIYHHEYFTMTRKMVYNNLAQELVMTIPLHEPLPTQYIVRAISDRWLGSEYMLPLTFHDLILPEVYPPYTDLLEVQPLPIKALKEPLFEKLYKFSHFNPIQTQIFHCLYHTDNNVLLGAPTGSGKTIAAEIAMFRVFKQYPTQKIVYIAPLKALVRERMKDWKIRFEEQLGKKVIELTGDVSPDIKVIANANIIITTPEKWDGISRSWQTRLYVKNVALIIIDEIHLLGEDRGPVLEVIISRTNFISSHTLKKLRVIGLSTALANAVDLANWLGIKEMGLYNFRPSVRPVPLDIHITGFPGKNYCPRMATMNRPTFQAIKQHAPTSPALVFVSSRRQTRLTALDLIAYLAGEDNPKQWLHMREEEMNNILSHIRDSNLKLTLAFGIGLHHAGFQERDRKIVEELFVNNKIQVLITTATLAWGVNFPAHLVVIKGTEYYDGKQHRYVDMPITDVLQMMGRAGRPQFDNSGVAVVLVHDLKKNYYKKFLHHPFPVESSLLAVLPDHINAEIVANTIKNKQELLDYLTWTYFFRRLMKNPRYYNLSILEPYAINQYLSSLVDNTLKVLIDSYCVAFDEEEQILYPLSMGKIASFYYLSHHTMLMFVQSLQESLTLEQCLHILCNSYEYNELPVRHNEELLNEELSKMCRYQIDNYSYNSPHTKAFLLLQAHFSRLPLPCVDYFTDLKSVLDQAIRIIQAMIDAVADHGWLANTIMIMNILQMIIQARWIDESAIITLPCVNSEHLELFSTLSLTLPELCFNMHNKDIKILRKVLNKSFSQEQIYQIYQVIKEMPMLCIKLSLESYDEDNDNNKQKNQIFIPLKSDNLDYINIHKDQDYILNIIMKRKNKSNSLKAHSPVFQKGKDEGWFLILGNVSDKELLVLYEHLQ

Target ORF information:

RefSeq Version	XM_006571832.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006571832.2

ORF Insert Sequence:

ATGTCTGTGG GCAACATTAA TCAAAGTACA GATAATTTCT CAATGAAATA TAAAAATGAA 
GCTACAAAAT TATTTAAAAC AGAATTTGAC AAGACAGCAT ATAAGGAAAA CTTGAAATCA 
TATCATACAA AGAATTTTGC AGATATATCT TTAGAAAAAT TTGAGAAGAT CATAATTACT 
AAATTAGGTT CTAAAAATTC AGAGAACGAA TTAGTTGATT TATTAGGTTG TGAATCTATA 
GAATTTATTC AATATATTGT TCAACATCAG AAAAGTATTA TAACTTTAAG TTCTACTCCA 
AAAATACAAA AAAAATGTAA TATTCAAGGA CCTGTGATCA GTGGCCAAGT AACAGTTCAA 
TCTGAGAAAG AAAAACAATT ACAAAAACAA ATACGGAAAC ACGAAAAAAA ATTAAATAAA 
ACGAGTAAAC GAGAAGGAAA GGGAGAGTTG GAAGAAAATG GCTTTGAACC ATTAGAATTG 
ACTTTAAAAA AAAATGAAGC ATTAATAGAA ATGCACACAC CTATATTTAA AAAAAGTATT 
AATACAGATC GAGAAAAATT TCCTTTTGTG TTTGATGCTA ATATGAACAG TAGAAATACA 
ACTGGTTATG TATCAAAACA AAAAATAATG TTACCGCAAA ATGTTATAAG GAAAGATACA 
GAAACATTTG AAGAAATTTT TATACCTATT CCAGAATCTC AACCACTTGA TGTAGATTAT 
AAACCTATTA TGGTATCTTC ATTGGATGAT ATTGGACAAA TGGCTTTTAA TGGCATTGAA 
TCATTAAATC GAATACAAAG TATTGTGTTC GATGTAGCAT ATCATACTAA TGAGAATTTA 
TTAATTTGTG CTCCAACAGG AGCTGGAAAG ACTAATGTTG CTATGTTAAC TATAGTACAT 
CAATTAAAGC AACATATTGA ATATGGTCAA TTAATGAAAA ATCAGTTCAA AATAATTTAT 
GTAACTCCAA TGAAAGCTTT AGCAGCTGAA ATGACAGCAA ATTTTAGCAA AAAACTTCAT 
TGTCTTGGTA TATCTGTTCG CGAGTTAACT GGAGATATGC AATTAACCAA ATCGGAAATT 
CAACAAACAC AGATGATTGT TACCACACCA GAAAAGTGGG ATGTTGTTAC TAGAAAAGGT 
ACTGGAGATA TTTCTCTCAC AAGTATTGTA AAACTGTTAA TTATTGATGA AGTACATTTG 
TTACATGGTG ATAGAGGACC TGTGGTTGAA GCCTTAGTTG CTCGAACTTT GAGACAAGTA 
GAATCTTCTC AAAGTATGAT CAGAATTGTT GGTCTTTCTG CAACATTGCC AAATTATGTA 
GATGTTGCAA GATTTTTAAG AGTGAATTCA AACATAGGAC TTTTTTATTT TGATCATCGA 
TTTCGACCAG TACCACTTAG TCAAGCGTTT ATTGGTGTAA AAGCATCATC ATCATTACAA 
GAAATGAATT ACATGGATAA TGTATGTTAC AATAATGTTA TAGATATGGT ACGTCAAGGA 
TATCAAGTAA TGGTATTTGT ACATGCACGA AATGCTACTG TTAGAATGGC AAATACACTA 
AAAGATTTAG CAATAAAACA TGGTACACTT AAATTATTTA TACCTGAAGG TCAAACAAAA 
TTTATAAATA AAGCTTTTGC TAAATCACGT ACGAAGTATT TAAGTGAATT ATTTAATAAT 
GGATTATCAG TACATCATGC TGGTTTATTA CGTTCAGAGA GAAATTTAAT TGAAAAATAT 
TTTGCAGATG GTTTAATTAA AGTATTAGTA TGTACGTCAA CATTAGCTTG GGGTGTAAAT 
CTACCTGCCC ATGCTGTTAT TATAAGAGGA ACAGAAATAT ATGACGCAAA ACATGGTTCT 
TATATTGACT TAGATATATT AGATGTTTTA CAAATTTTTG GTCGTGCTGG TAGACCACAA 
TTTGATGTAT CTGGTCATGC TGTTATTATT ACTTCCCATA ATAAATTATA CCATTACTTA 
TCATTATTAA CAAATCAAAT ACCTATAGAA AGCAATTTCA TTAAATATTT AGCTGATAAT 
TTAAATGCTG AAATTGCATT GGGTACAATA TCGAATGTAG AAGAAGCAAT AAAATGGTTA 
AGTTATACTT ATTTATTCGT TCGAATGAAA TTAAATTTTC ATTTTTATGG AATTCCTTAT 
CGAGTTATTT TGGAAGATCA AAATTTAGAA CAAAAAAGAA GAGAACTAAT TGATCAAGCA 
GCTAAAGCAT TAGATGAAGC TAAAATGATT CGATATAATA TAAATACTGG AGATCTGAGC 
ACAACAAATT TAGGACGAAT TGCGAGCCAT TTTTATCTTA AATATGACAC TATTAAAATT 
TTTAATACAC TTCTAAAACC AATTATGACT GAAGCAGAAA TATTTTCTAT GATATCATAT 
TCTCAAGAAT TTGAACAATT AAAAGTGCGA GATGATGAAA TGGAGGAATT ACAATATTTA 
TTTGAAGAAT ATTGTAATAT TACAGTCCAA GGTGGTGTTG AAAATATATA TGGTAAAGTT 
AATATATTAT TACAAACGTA TTTGTCACAT GGTAGAGTTA ATGCGTTTTC ATTAATATCT 
GATCAAGCAT ATATTGTACA AAATGCAGGA CGGATATTTC GAGCGTTGTT TGAAATTATG 
TTAGGACAAA ATAATGCAAT AATGGCTGGT CGTTTATTAG AAATAGCAAA AGTACTCGAA 
ATACAACAAT GGAGTGATAG AAGTCCTCTA TGTCAATTTT CTTGTTTATC TTTAGAAATT 
ATTGACAAGA TAGAACAATA TGATTTAACA GTTGAAAGAT TAAATAATAT GAATGTTAAA 
GAAATTGGAG ATATTTTGCG TAATCAGAAA GAAGCAATAT TGGTGAAAAA ATGTTGCAAA 
GAATTACCTG CATTAGAAAT GGAATCAAAT TTACAACCTA TTACTCGTAC AGTTTTAAGA 
ATACATTTAA AAATATATCC TCAATTTCAT TGGAATGATA ATGTTCATGG TAAAACTTCA 
GAACCATTCT GGATATGGAT AGAAGATCCA GATAATAATT TTATTTATCA TCATGAATAT 
TTTACTATGA CAAGAAAAAT GGTTTACAAT AATCTTGCAC AAGAACTTGT GATGACAATA 
CCATTGCATG AACCATTGCC TACTCAATAC ATAGTAAGAG CAATAAGTGA TCGCTGGTTG 
GGTTCTGAAT ATATGTTACC TTTAACATTT CATGATTTAA TTTTACCTGA AGTATATCCA 
CCATATACTG ATTTGTTAGA AGTACAACCT CTGCCAATAA AAGCATTGAA AGAACCATTA 
TTTGAAAAAC TTTATAAATT TTCTCATTTT AATCCAATAC AGACACAAAT TTTTCATTGT 
TTGTATCATA CAGACAATAA CGTCCTATTA GGAGCTCCAA CTGGTTCAGG AAAAACAATT 
GCTGCGGAAA TTGCAATGTT TAGAGTATTT AAACAATATC CAACGCAAAA AATTGTATAT 
ATTGCACCAT TGAAAGCTTT AGTAAGAGAA CGAATGAAAG ATTGGAAAAT TAGATTTGAA 
GAACAATTAG GAAAAAAAGT AATAGAATTA ACTGGAGATG TATCACCAGA TATTAAAGTT 
ATAGCAAATG CAAATATTAT TATAACTACT CCAGAAAAAT GGGATGGTAT TAGTAGAAGT 
TGGCAAACAA GACTTTATGT CAAAAATGTT GCGCTTATTA TTATAGATGA AATACATTTA 
TTAGGGGAAG ATCGTGGACC AGTATTAGAA GTTATTATTT CAAGAACTAA TTTTATTTCT 
AGTCATACTT TAAAAAAATT AAGAGTTATT GGACTTTCTA CAGCATTAGC TAATGCAGTA 
GATTTAGCTA ATTGGCTTGG TATTAAAGAG ATGGGTCTTT ATAATTTTCG TCCATCTGTA 
CGACCTGTAC CATTAGATAT TCATATAACT GGATTCCCAG GGAAAAATTA TTGTCCTCGA 
ATGGCAACAA TGAATAGACC AACTTTTCAA GCAATTAAAC AACATGCTCC AACTAGTCCG 
GCTTTAGTAT TTGTTTCATC ACGTAGACAA ACACGTTTAA CTGCATTAGA TTTGATTGCA 
TATCTTGCAG GAGAAGATAA TCCTAAGCAA TGGTTACATA TGAGAGAAGA AGAAATGAAT 
AACATTCTAA GTCATATACG AGATTCAAAT TTGAAACTTA CACTTGCTTT TGGAATTGGA 
CTTCATCATG CAGGTTTTCA GGAAAGAGAT AGAAAAATTG TCGAAGAATT ATTTGTTAAC 
AATAAAATTC AGGTATTGAT TACAACAGCT ACTTTAGCTT GGGGAGTTAA TTTCCCTGCA 
CACTTAGTTG TAATAAAAGG AACAGAATAT TATGATGGCA AACAACACCG TTATGTAGAC 
ATGCCAATCA CAGATGTACT ACAAATGATG GGTCGTGCAG GAAGACCCCA GTTTGATAAT 
TCAGGAGTGG CAGTAGTTTT AGTACACGAT TTAAAAAAAA ATTATTATAA GAAATTTTTA 
CATCATCCAT TTCCAGTAGA ATCAAGTTTA TTAGCTGTTC TGCCAGATCA TATAAATGCT 
GAAATTGTAG CTAATACAAT TAAAAATAAA CAAGAATTAT TAGATTATTT AACATGGACA 
TATTTTTTTA GAAGGTTAAT GAAAAATCCT AGATATTACA ATTTAAGTAT TTTAGAACCT 
TATGCTATTA ATCAATATCT CTCTTCTTTG GTTGATAATA CACTAAAAGT ATTAATAGAT 
TCATATTGTG TGGCATTTGA TGAGGAAGAA CAAATTTTAT ATCCACTTTC AATGGGTAAA 
ATAGCATCAT TTTATTACTT ATCGCATCAT ACTATGTTAA TGTTTGTACA ATCATTACAA 
GAATCTCTTA CATTGGAGCA ATGTTTACAT ATTTTATGTA ATTCTTATGA ATATAATGAA 
TTACCAGTAA GACACAATGA AGAATTATTG AATGAGGAAT TAAGTAAAAT GTGCCGTTAT 
CAAATAGATA ATTACTCATA TAATTCTCCT CATACCAAAG CATTTTTATT ACTTCAAGCA 
CATTTCTCAA GACTTCCCTT ACCATGTGTA GATTATTTTA CTGATTTAAA ATCAGTTCTT 
GATCAGGCAA TTAGGATTAT ACAAGCAATG ATAGATGCAG TTGCAGATCA TGGATGGTTA 
GCAAATACAA TTATGATAAT GAACATACTT CAAATGATTA TTCAAGCTCG ATGGATTGAT 
GAATCAGCAA TTATTACATT GCCATGTGTA AATTCAGAAC ATTTAGAATT ATTTTCAACA 
TTATCGCTTA CTCTTCCTGA ATTATGTTTC AATATGCACA ACAAAGATAT TAAAATACTT 
AGAAAAGTAC TTAATAAAAG TTTTTCGCAA GAACAAATAT ATCAGATATA TCAAGTAATA 
AAAGAAATGC CAATGCTCTG TATTAAGTTA AGCTTAGAAA GTTATGATGA AGATAATGAT 
AATAATAAAC AAAAAAATCA AATATTTATA CCTCTAAAAT CTGATAATTT GGATTATATA 
AATATTCACA AGGATCAAGA TTACATATTA AATATTATAA TGAAAAGAAA AAATAAATCT 
AATAGTTTAA AAGCACACAG TCCTGTGTTT CAAAAAGGAA AGGATGAAGG TTGGTTTTTA 
ATTTTAGGTA ATGTTTCTGA TAAAGAATTA TTAGTTCTAT ACGAGCATCT GCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552814 ( XM_026443881.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026299666.1 Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X2, mRNA.

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CloneID	OHd25552
Clone ID Related Accession (Same CDS sequence)	XM_026443881.1 , XM_026443880.1 , XM_026443882.1
Accession Version	XM_026443881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	activating signal cointegrator 1 complex subunit 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037648.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGAAT TACCTCGAAT TACAAGATCG TTACGAATGT TCACGAATCT TGGACGTATA 
AAAAGTACAA AGGAACTAGA ACCAAATGAT TTATTTAGAA AAGAAAAAGA GCAACGTAGC 
AAATCTAAAA GTGAATTCAA TTGGCAAGAT TTATGTAAAC ATGTATCTAA AAATGTTTTA 
TTTCATCTTA ATAAACTACG CCAAATTGCT ACAGAAGCAC TTGGGGAATG TTCTTTTGAA 
CTTATAAATG AATTTTTAAT GTTTACTTTA CAATTATTAA TTGATGACAA GTTGTTAACT 
CAAATAAAAT TTAAAATTTT AAAAGAAAAA GCAGGAAATT TAAAATTCCA TGATGCTAAC 
CACATGATGA AGATAATTAC AGAAATTCGA GAAAAATTAA ACGAAGAAAC TTTAATACTT 
ATAAAAAACG GAAAAGAAAA TATTAAAGTA TTGGAAAATG GCTTATTTGA TCCAAAATTT 
GAATATAAAT CTGCAAGAGT ATTATGGCCT GATACTAATA CACTTCGTAG TATGTCTGTG 
GGCAACATTA ATCAAAGTAC AGATAATTTC TCAATGAAAT ATAAAAATGA AGCTACAAAA 
TTATTTAAAA CAGAATTTGA CAAGACAGCA TATAAGGAAA ACTTGAAATC ATATCATACA 
AAGAATTTTG CAGATATATC TTTAGAAAAA TTTGAGAAGA TCATAATTAC TAAATTAGGT 
TCTAAAAATT CAGAGAACGA ATTAGTTGAT TTATTAGGTT GTGAATCTAT AGAATTTATT 
CAATATATTG TTCAACATCA GAAAAGTATT ATAACTTTAA GTTCTACTCC AAAAATACAA 
AAAAAATGTA ATATTCAAGG ACCTGTGATC AGTGGCCAAG TAACAGTTCA ATCTGAGAAA 
GAAAAACAAT TACAAAAACA AATACGGAAA CACGAAAAAA AATTAAATAA AACGAGTAAA 
CGAGAAGGAA AGGGAGAGTT GGAAGAAAAT GGCTTTGAAC CATTAGAATT GACTTTAAAA 
AAAAATGAAG CATTAATAGA AATGCACACA CCTATATTTA AAAAAAGTAT TAATACAGAT 
CGAGAAAAAT TTCCTTTTGT GTTTGATGCT AATATGAACA GTAGAAATAC AACTGGTTAT 
GTATCAAAAC AAAAAATAAT GTTACCGCAA AATGTTATAA GGAAAGATAC AGAAACATTT 
GAAGAAATTT TTATACCTAT TCCAGAATCT CAACCACTTG ATGTAGATTA TAAACCTATT 
ATGGTATCTT CATTGGATGA TATTGGACAA ATGGCTTTTA ATGGCATTGA ATCATTAAAT 
CGAATACAAA GTATTGTGTT CGATGTAGCA TATCATACTA ATGAGAATTT ATTAATTTGT 
GCTCCAACAG GAGCTGGAAA GACTAATGTT GCTATGTTAA CTATAGTACA TCAATTAAAG 
CAACATATTG AATATGGTCA ATTAATGAAA AATCAGTTCA AAATAATTTA TGTAACTCCA 
ATGAAAGCTT TAGCAGCTGA AATGACAGCA AATTTTAGCA AAAAACTTCA TTGTCTTGGT 
ATATCTGTTC GCGAGTTAAC TGGAGATATG CAATTAACCA AATCGGAAAT TCAACAAACA 
CAGATGATTG TTACCACACC AGAAAAGTGG GATGTTGTTA CTAGAAAAGG TACTGGAGAT 
ATTTCTCTCA CAAGTATTGT AAAACTGTTA ATTATTGATG AAGTACATTT GTTACATGGT 
GATAGAGGAC CTGTGGTTGA AGCCTTAGTT GCTCGAACTT TGAGACAAGT AGAATCTTCT 
CAAAGTATGA TCAGAATTGT TGGTCTTTCT GCAACATTGC CAAATTATGT AGATGTTGCA 
AGATTTTTAA GAGTGAATTC AAACATAGGA CTTTTTTATT TTGATCATCG ATTTCGACCA 
GTACCACTTA GTCAAGCGTT TATTGGTGTA AAAGCATCAT CATCATTACA AGAAATGAAT 
TACATGGATA ATGTATGTTA CAATAATGTT ATAGATATGG TACGTCAAGG ATATCAAGTA 
ATGGTATTTG TACATGCACG AAATGCTACT GTTAGAATGG CAAATACACT AAAAGATTTA 
GCAATAAAAC ATGGTACACT TAAATTATTT ATACCTGAAG GTCAAACAAA ATTTATAAAT 
AAAGCTTTTG CTAAATCACG TACGAAGTAT TTAAGTGAAT TATTTAATAA TGGATTATCA 
GTACATCATG CTGGTTTATT ACGTTCAGAG AGAAATTTAA TTGAAAAATA TTTTGCAGAT 
GGTTTAATTA AAGTATTAGT ATGTACGTCA ACATTAGCTT GGGGTGTAAA TCTACCTGCC 
CATGCTGTTA TTATAAGAGG AACAGAAATA TATGACGCAA AACATGGTTC TTATATTGAC 
TTAGATATAT TAGATGTTTT ACAAATTTTT GGTCGTGCTG GTAGACCACA ATTTGATGTA 
TCTGGTCATG CTGTTATTAT TACTTCCCAT AATAAATTAT ACCATTACTT ATCATTATTA 
ACAAATCAAA TACCTATAGA AAGCAATTTC ATTAAATATT TAGCTGATAA TTTAAATGCT 
GAAATTGCAT TGGGTACAAT ATCGAATGTA GAAGAAGCAA TAAAATGGTT AAGTTATACT 
TATTTATTCG TTCGAATGAA ATTAAATTTT CATTTTTATG GAATTCCTTA TCGAGTTATT 
TTGGAAGATC AAAATTTAGA ACAAAAAAGA AGAGAACTAA TTGATCAAGC AGCTAAAGCA 
TTAGATGAAG CTAAAATGAT TCGATATAAT ATAAATACTG GAGATCTGAG CACAACAAAT 
TTAGGACGAA TTGCGAGCCA TTTTTATCTT AAATATGACA CTATTAAAAT TTTTAATACA 
CTTCTAAAAC CAATTATGAC TGAAGCAGAA ATATTTTCTA TGATATCATA TTCTCAAGAA 
TTTGAACAAT TAAAAGTGCG AGATGATGAA ATGGAGGAAT TACAATATTT ATTTGAAGAA 
TATTGTAATA TTACAGTCCA AGGTGGTGTT GAAAATATAT ATGGTAAAGT TAATATATTA 
TTACAAACGT ATTTGTCACA TGGTAGAGTT AATGCGTTTT CATTAATATC TGATCAAGCA 
TATATTGTAC AAAATGCAGG ACGGATATTT CGAGCGTTGT TTGAAATTAT GTTAGGACAA 
AATAATGCAA TAATGGCTGG TCGTTTATTA GAAATAGCAA AAGTACTCGA AATACAACAA 
TGGAGTGATA GAAGTCCTCT ATGTCAATTT TCTTGTTTAT CTTTAGAAAT TATTGACAAG 
ATAGAACAAT ATGATTTAAC AGTTGAAAGA TTAAATAATA TGAATGTTAA AGAAATTGGA 
GATATTTTGC GTAATCAGAA AGAAGCAATA TTGGTGAAAA AATGTTGCAA AGAATTACCT 
GCATTAGAAA TGGAATCAAA TTTACAACCT ATTACTCGTA CAGTTTTAAG AATACATTTA 
AAAATATATC CTCAATTTCA TTGGAATGAT AATGTTCATG GTAAAACTTC AGAACCATTC 
TGGATATGGA TAGAAGATCC AGATAATAAT TTTATTTATC ATCATGAATA TTTTACTATG 
ACAAGAAAAA TGGTTTACAA TAATCTTGCA CAAGAACTTG TGATGACAAT ACCATTGCAT 
GAACCATTGC CTACTCAATA CATAGTAAGA GCAATAAGTG ATCGCTGGTT GGGTTCTGAA 
TATATGTTAC CTTTAACATT TCATGATTTA ATTTTACCTG AAGTATATCC ACCATATACT 
GATTTGTTAG AAGTACAACC TCTGCCAATA AAAGCATTGA AAGAACCATT ATTTGAAAAA 
CTTTATAAAT TTTCTCATTT TAATCCAATA CAGACACAAA TTTTTCATTG TTTGTATCAT 
ACAGACAATA ACGTCCTATT AGGAGCTCCA ACTGGTTCAG GAAAAACAAT TGCTGCGGAA 
ATTGCAATGT TTAGAGTATT TAAACAATAT CCAACGCAAA AAATTGTATA TATTGCACCA 
TTGAAAGCTT TAGTAAGAGA ACGAATGAAA GATTGGAAAA TTAGATTTGA AGAACAATTA 
GGAAAAAAAG TAATAGAATT AACTGGAGAT GTATCACCAG ATATTAAAGT TATAGCAAAT 
GCAAATATTA TTATAACTAC TCCAGAAAAA TGGGATGGTA TTAGTAGAAG TTGGCAAACA 
AGACTTTATG TCAAAAATGT TGCGCTTATT ATTATAGATG AAATACATTT ATTAGGGGAA 
GATCGTGGAC CAGTATTAGA AGTTATTATT TCAAGAACTA ATTTTATTTC TAGTCATACT 
TTAAAAAAAT TAAGAGTTAT TGGACTTTCT ACAGCATTAG CTAATGCAGT AGATTTAGCT 
AATTGGCTTG GTATTAAAGA GATGGGTCTT TATAATTTTC GTCCATCTGT ACGACCTGTA 
CCATTAGATA TTCATATAAC TGGATTCCCA GGGAAAAATT ATTGTCCTCG AATGGCAACA 
ATGAATAGAC CAACTTTTCA AGCAATTAAA CAACATGCTC CAACTAGTCC GGCTTTAGTA 
TTTGTTTCAT CACGTAGACA AACACGTTTA ACTGCATTAG ATTTGATTGC ATATCTTGCA 
GGAGAAGATA ATCCTAAGCA ATGGTTACAT ATGAGAGAAG AAGAAATGAA TAACATTCTA 
AGTCATATAC GAGATTCAAA TTTGAAACTT ACACTTGCTT TTGGAATTGG ACTTCATCAT 
GCAGGTTTTC AGGAAAGAGA TAGAAAAATT GTCGAAGAAT TATTTGTTAA CAATAAAATT 
CAGGTATTGA TTACAACAGC TACTTTAGCT TGGGGAGTTA ATTTCCCTGC ACACTTAGTT 
GTAATAAAAG GAACAGAATA TTATGATGGC AAACAACACC GTTATGTAGA CATGCCAATC 
ACAGATGTAC TACAAATGAT GGGTCGTGCA GGAAGACCCC AGTTTGATAA TTCAGGAGTG 
GCAGTAGTTT TAGTACACGA TTTAAAAAAA AATTATTATA AGAAATTTTT ACATCATCCA 
TTTCCAGTAG AATCAAGTTT ATTAGCTGTT CTGCCAGATC ATATAAATGC TGAAATTGTA 
GCTAATACAA TTAAAAATAA ACAAGAATTA TTAGATTATT TAACATGGAC ATATTTTTTT 
AGAAGGTTAA TGAAAAATCC TAGATATTAC AATTTAAGTA TTTTAGAACC TTATGCTATT 
AATCAATATC TCTCTTCTTT GGTTGATAAT ACACTAAAAG TATTAATAGA TTCATATTGT 
GTGGCATTTG ATGAGGAAGA ACAAATTTTA TATCCACTTT CAATGGGTAA AATAGCATCA 
TTTTATTACT TATCGCATCA TACTATGTTA ATGTTTGTAC AATCATTACA AGAATCTCTT 
ACATTGGAGC AATGTTTACA TATTTTATGT AATTCTTATG AATATAATGA ATTACCAGTA 
AGACACAATG AAGAATTATT GAATGAGGAA TTAAGTAAAA TGTGCCGTTA TCAAATAGAT 
AATTACTCAT ATAATTCTCC TCATACCAAA GCATTTTTAT TACTTCAAGC ACATTTCTCA 
AGACTTCCCT TACCATGTGT AGATTATTTT ACTGATTTAA AATCAGTTCT TGATCAGGCA 
ATTAGGATTA TACAAGCAAT GATAGATGCA GTTGCAGATC ATGGATGGTT AGCAAATACA 
ATTATGATAA TGAACATACT TCAAATGATT ATTCAAGCTC GATGGATTGA TGAATCAGCA 
ATTATTACAT TGCCATGTGT AAATTCAGAA CATTTAGAAT TATTTTCAAC ATTATCGCTT 
ACTCTTCCTG AATTATGTTT CAATATGCAC AACAAAGATA TTAAAATACT TAGAAAAGTA 
CTTAATAAAA GTTTTTCGCA AGAACAAATA TATCAGATAT ATCAAGTAAT AAAAGAAATG 
CCAATGCTCT GTATTAAGTT AAGCTTAGAA AGTTATGATG AAGATAATGA TAATAATAAA 
CAAAAAAATC AAATATTTAT ACCTCTAAAA TCTGATAATT TGGATTATAT AAATATTCAC 
AAGGATCAAG ATTACATATT AAATATTATA ATGAAAAGAA AAAATAAATC TAATAATTTA 
AAAGCACACT GTCCTTTGTT TCAAAAAGGA AAGGATGAAG GTTGGTTTTT AATTTTAGGT 
AATGTTTCTG ATAAAGAATT ATTAGTTCTA AAACGAGCAT CTGCAATAAA TGAACAACGG 
AAATATCAAT TACAATTCAC AGCTCCATCA AAATTAGGAC AAACAACTTT AACGTTTTAT 
TTGATTTCCG ATTGTTACAT AGGACTGGAT CAACAGTATG ATATAAAAAT AAATGTAATA 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026299666.1
CDS	298..6663
Translation	MPELPRITRSLRMFTNLGRIKSTKELEPNDLFRKEKEQRSKSKSEFNWQDLCKHVSKNVLFHLNKLRQIATEALGECSFELINEFLMFTLQLLIDDKLLTQIKFKILKEKAGNLKFHDANHMMKIITEIREKLNEETLILIKNGKENIKVLENGLFDPKFEYKSARVLWPDTNTLRSMSVGNINQSTDNFSMKYKNEATKLFKTEFDKTAYKENLKSYHTKNFADISLEKFEKIIITKLGSKNSENELVDLLGCESIEFIQYIVQHQKSIITLSSTPKIQKKCNIQGPVISGQVTVQSEKEKQLQKQIRKHEKKLNKTSKREGKGELEENGFEPLELTLKKNEALIEMHTPIFKKSINTDREKFPFVFDANMNSRNTTGYVSKQKIMLPQNVIRKDTETFEEIFIPIPESQPLDVDYKPIMVSSLDDIGQMAFNGIESLNRIQSIVFDVAYHTNENLLICAPTGAGKTNVAMLTIVHQLKQHIEYGQLMKNQFKIIYVTPMKALAAEMTANFSKKLHCLGISVRELTGDMQLTKSEIQQTQMIVTTPEKWDVVTRKGTGDISLTSIVKLLIIDEVHLLHGDRGPVVEALVARTLRQVESSQSMIRIVGLSATLPNYVDVARFLRVNSNIGLFYFDHRFRPVPLSQAFIGVKASSSLQEMNYMDNVCYNNVIDMVRQGYQVMVFVHARNATVRMANTLKDLAIKHGTLKLFIPEGQTKFINKAFAKSRTKYLSELFNNGLSVHHAGLLRSERNLIEKYFADGLIKVLVCTSTLAWGVNLPAHAVIIRGTEIYDAKHGSYIDLDILDVLQIFGRAGRPQFDVSGHAVIITSHNKLYHYLSLLTNQIPIESNFIKYLADNLNAEIALGTISNVEEAIKWLSYTYLFVRMKLNFHFYGIPYRVILEDQNLEQKRRELIDQAAKALDEAKMIRYNINTGDLSTTNLGRIASHFYLKYDTIKIFNTLLKPIMTEAEIFSMISYSQEFEQLKVRDDEMEELQYLFEEYCNITVQGGVENIYGKVNILLQTYLSHGRVNAFSLISDQAYIVQNAGRIFRALFEIMLGQNNAIMAGRLLEIAKVLEIQQWSDRSPLCQFSCLSLEIIDKIEQYDLTVERLNNMNVKEIGDILRNQKEAILVKKCCKELPALEMESNLQPITRTVLRIHLKIYPQFHWNDNVHGKTSEPFWIWIEDPDNNFIYHHEYFTMTRKMVYNNLAQELVMTIPLHEPLPTQYIVRAISDRWLGSEYMLPLTFHDLILPEVYPPYTDLLEVQPLPIKALKEPLFEKLYKFSHFNPIQTQIFHCLYHTDNNVLLGAPTGSGKTIAAEIAMFRVFKQYPTQKIVYIAPLKALVRERMKDWKIRFEEQLGKKVIELTGDVSPDIKVIANANIIITTPEKWDGISRSWQTRLYVKNVALIIIDEIHLLGEDRGPVLEVIISRTNFISSHTLKKLRVIGLSTALANAVDLANWLGIKEMGLYNFRPSVRPVPLDIHITGFPGKNYCPRMATMNRPTFQAIKQHAPTSPALVFVSSRRQTRLTALDLIAYLAGEDNPKQWLHMREEEMNNILSHIRDSNLKLTLAFGIGLHHAGFQERDRKIVEELFVNNKIQVLITTATLAWGVNFPAHLVVIKGTEYYDGKQHRYVDMPITDVLQMMGRAGRPQFDNSGVAVVLVHDLKKNYYKKFLHHPFPVESSLLAVLPDHINAEIVANTIKNKQELLDYLTWTYFFRRLMKNPRYYNLSILEPYAINQYLSSLVDNTLKVLIDSYCVAFDEEEQILYPLSMGKIASFYYLSHHTMLMFVQSLQESLTLEQCLHILCNSYEYNELPVRHNEELLNEELSKMCRYQIDNYSYNSPHTKAFLLLQAHFSRLPLPCVDYFTDLKSVLDQAIRIIQAMIDAVADHGWLANTIMIMNILQMIIQARWIDESAIITLPCVNSEHLELFSTLSLTLPELCFNMHNKDIKILRKVLNKSFSQEQIYQIYQVIKEMPMLCIKLSLESYDEDNDNNKQKNQIFIPLKSDNLDYINIHKDQDYILNIIMKRKNKSNNLKAHCPLFQKGKDEGWFLILGNVSDKELLVLKRASAINEQRKYQLQFTAPSKLGQTTLTFYLISDCYIGLDQQYDIKINVIS

Target ORF information:

RefSeq Version	XM_026443881.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026443881.1

ORF Insert Sequence:

ATGCCAGAAT TACCTCGAAT TACAAGATCG TTACGAATGT TCACGAATCT TGGACGTATA 
AAAAGTACAA AGGAACTAGA ACCAAATGAT TTATTTAGAA AAGAAAAAGA GCAACGTAGC 
AAATCTAAAA GTGAATTCAA TTGGCAAGAT TTATGTAAAC ATGTATCTAA AAATGTTTTA 
TTTCATCTTA ATAAACTACG CCAAATTGCT ACAGAAGCAC TTGGGGAATG TTCTTTTGAA 
CTTATAAATG AATTTTTAAT GTTTACTTTA CAATTATTAA TTGATGACAA GTTGTTAACT 
CAAATAAAAT TTAAAATTTT AAAAGAAAAA GCAGGAAATT TAAAATTCCA TGATGCTAAC 
CACATGATGA AGATAATTAC AGAAATTCGA GAAAAATTAA ACGAAGAAAC TTTAATACTT 
ATAAAAAACG GAAAAGAAAA TATTAAAGTA TTGGAAAATG GCTTATTTGA TCCAAAATTT 
GAATATAAAT CTGCAAGAGT ATTATGGCCT GATACTAATA CACTTCGTAG TATGTCTGTG 
GGCAACATTA ATCAAAGTAC AGATAATTTC TCAATGAAAT ATAAAAATGA AGCTACAAAA 
TTATTTAAAA CAGAATTTGA CAAGACAGCA TATAAGGAAA ACTTGAAATC ATATCATACA 
AAGAATTTTG CAGATATATC TTTAGAAAAA TTTGAGAAGA TCATAATTAC TAAATTAGGT 
TCTAAAAATT CAGAGAACGA ATTAGTTGAT TTATTAGGTT GTGAATCTAT AGAATTTATT 
CAATATATTG TTCAACATCA GAAAAGTATT ATAACTTTAA GTTCTACTCC AAAAATACAA 
AAAAAATGTA ATATTCAAGG ACCTGTGATC AGTGGCCAAG TAACAGTTCA ATCTGAGAAA 
GAAAAACAAT TACAAAAACA AATACGGAAA CACGAAAAAA AATTAAATAA AACGAGTAAA 
CGAGAAGGAA AGGGAGAGTT GGAAGAAAAT GGCTTTGAAC CATTAGAATT GACTTTAAAA 
AAAAATGAAG CATTAATAGA AATGCACACA CCTATATTTA AAAAAAGTAT TAATACAGAT 
CGAGAAAAAT TTCCTTTTGT GTTTGATGCT AATATGAACA GTAGAAATAC AACTGGTTAT 
GTATCAAAAC AAAAAATAAT GTTACCGCAA AATGTTATAA GGAAAGATAC AGAAACATTT 
GAAGAAATTT TTATACCTAT TCCAGAATCT CAACCACTTG ATGTAGATTA TAAACCTATT 
ATGGTATCTT CATTGGATGA TATTGGACAA ATGGCTTTTA ATGGCATTGA ATCATTAAAT 
CGAATACAAA GTATTGTGTT CGATGTAGCA TATCATACTA ATGAGAATTT ATTAATTTGT 
GCTCCAACAG GAGCTGGAAA GACTAATGTT GCTATGTTAA CTATAGTACA TCAATTAAAG 
CAACATATTG AATATGGTCA ATTAATGAAA AATCAGTTCA AAATAATTTA TGTAACTCCA 
ATGAAAGCTT TAGCAGCTGA AATGACAGCA AATTTTAGCA AAAAACTTCA TTGTCTTGGT 
ATATCTGTTC GCGAGTTAAC TGGAGATATG CAATTAACCA AATCGGAAAT TCAACAAACA 
CAGATGATTG TTACCACACC AGAAAAGTGG GATGTTGTTA CTAGAAAAGG TACTGGAGAT 
ATTTCTCTCA CAAGTATTGT AAAACTGTTA ATTATTGATG AAGTACATTT GTTACATGGT 
GATAGAGGAC CTGTGGTTGA AGCCTTAGTT GCTCGAACTT TGAGACAAGT AGAATCTTCT 
CAAAGTATGA TCAGAATTGT TGGTCTTTCT GCAACATTGC CAAATTATGT AGATGTTGCA 
AGATTTTTAA GAGTGAATTC AAACATAGGA CTTTTTTATT TTGATCATCG ATTTCGACCA 
GTACCACTTA GTCAAGCGTT TATTGGTGTA AAAGCATCAT CATCATTACA AGAAATGAAT 
TACATGGATA ATGTATGTTA CAATAATGTT ATAGATATGG TACGTCAAGG ATATCAAGTA 
ATGGTATTTG TACATGCACG AAATGCTACT GTTAGAATGG CAAATACACT AAAAGATTTA 
GCAATAAAAC ATGGTACACT TAAATTATTT ATACCTGAAG GTCAAACAAA ATTTATAAAT 
AAAGCTTTTG CTAAATCACG TACGAAGTAT TTAAGTGAAT TATTTAATAA TGGATTATCA 
GTACATCATG CTGGTTTATT ACGTTCAGAG AGAAATTTAA TTGAAAAATA TTTTGCAGAT 
GGTTTAATTA AAGTATTAGT ATGTACGTCA ACATTAGCTT GGGGTGTAAA TCTACCTGCC 
CATGCTGTTA TTATAAGAGG AACAGAAATA TATGACGCAA AACATGGTTC TTATATTGAC 
TTAGATATAT TAGATGTTTT ACAAATTTTT GGTCGTGCTG GTAGACCACA ATTTGATGTA 
TCTGGTCATG CTGTTATTAT TACTTCCCAT AATAAATTAT ACCATTACTT ATCATTATTA 
ACAAATCAAA TACCTATAGA AAGCAATTTC ATTAAATATT TAGCTGATAA TTTAAATGCT 
GAAATTGCAT TGGGTACAAT ATCGAATGTA GAAGAAGCAA TAAAATGGTT AAGTTATACT 
TATTTATTCG TTCGAATGAA ATTAAATTTT CATTTTTATG GAATTCCTTA TCGAGTTATT 
TTGGAAGATC AAAATTTAGA ACAAAAAAGA AGAGAACTAA TTGATCAAGC AGCTAAAGCA 
TTAGATGAAG CTAAAATGAT TCGATATAAT ATAAATACTG GAGATCTGAG CACAACAAAT 
TTAGGACGAA TTGCGAGCCA TTTTTATCTT AAATATGACA CTATTAAAAT TTTTAATACA 
CTTCTAAAAC CAATTATGAC TGAAGCAGAA ATATTTTCTA TGATATCATA TTCTCAAGAA 
TTTGAACAAT TAAAAGTGCG AGATGATGAA ATGGAGGAAT TACAATATTT ATTTGAAGAA 
TATTGTAATA TTACAGTCCA AGGTGGTGTT GAAAATATAT ATGGTAAAGT TAATATATTA 
TTACAAACGT ATTTGTCACA TGGTAGAGTT AATGCGTTTT CATTAATATC TGATCAAGCA 
TATATTGTAC AAAATGCAGG ACGGATATTT CGAGCGTTGT TTGAAATTAT GTTAGGACAA 
AATAATGCAA TAATGGCTGG TCGTTTATTA GAAATAGCAA AAGTACTCGA AATACAACAA 
TGGAGTGATA GAAGTCCTCT ATGTCAATTT TCTTGTTTAT CTTTAGAAAT TATTGACAAG 
ATAGAACAAT ATGATTTAAC AGTTGAAAGA TTAAATAATA TGAATGTTAA AGAAATTGGA 
GATATTTTGC GTAATCAGAA AGAAGCAATA TTGGTGAAAA AATGTTGCAA AGAATTACCT 
GCATTAGAAA TGGAATCAAA TTTACAACCT ATTACTCGTA CAGTTTTAAG AATACATTTA 
AAAATATATC CTCAATTTCA TTGGAATGAT AATGTTCATG GTAAAACTTC AGAACCATTC 
TGGATATGGA TAGAAGATCC AGATAATAAT TTTATTTATC ATCATGAATA TTTTACTATG 
ACAAGAAAAA TGGTTTACAA TAATCTTGCA CAAGAACTTG TGATGACAAT ACCATTGCAT 
GAACCATTGC CTACTCAATA CATAGTAAGA GCAATAAGTG ATCGCTGGTT GGGTTCTGAA 
TATATGTTAC CTTTAACATT TCATGATTTA ATTTTACCTG AAGTATATCC ACCATATACT 
GATTTGTTAG AAGTACAACC TCTGCCAATA AAAGCATTGA AAGAACCATT ATTTGAAAAA 
CTTTATAAAT TTTCTCATTT TAATCCAATA CAGACACAAA TTTTTCATTG TTTGTATCAT 
ACAGACAATA ACGTCCTATT AGGAGCTCCA ACTGGTTCAG GAAAAACAAT TGCTGCGGAA 
ATTGCAATGT TTAGAGTATT TAAACAATAT CCAACGCAAA AAATTGTATA TATTGCACCA 
TTGAAAGCTT TAGTAAGAGA ACGAATGAAA GATTGGAAAA TTAGATTTGA AGAACAATTA 
GGAAAAAAAG TAATAGAATT AACTGGAGAT GTATCACCAG ATATTAAAGT TATAGCAAAT 
GCAAATATTA TTATAACTAC TCCAGAAAAA TGGGATGGTA TTAGTAGAAG TTGGCAAACA 
AGACTTTATG TCAAAAATGT TGCGCTTATT ATTATAGATG AAATACATTT ATTAGGGGAA 
GATCGTGGAC CAGTATTAGA AGTTATTATT TCAAGAACTA ATTTTATTTC TAGTCATACT 
TTAAAAAAAT TAAGAGTTAT TGGACTTTCT ACAGCATTAG CTAATGCAGT AGATTTAGCT 
AATTGGCTTG GTATTAAAGA GATGGGTCTT TATAATTTTC GTCCATCTGT ACGACCTGTA 
CCATTAGATA TTCATATAAC TGGATTCCCA GGGAAAAATT ATTGTCCTCG AATGGCAACA 
ATGAATAGAC CAACTTTTCA AGCAATTAAA CAACATGCTC CAACTAGTCC GGCTTTAGTA 
TTTGTTTCAT CACGTAGACA AACACGTTTA ACTGCATTAG ATTTGATTGC ATATCTTGCA 
GGAGAAGATA ATCCTAAGCA ATGGTTACAT ATGAGAGAAG AAGAAATGAA TAACATTCTA 
AGTCATATAC GAGATTCAAA TTTGAAACTT ACACTTGCTT TTGGAATTGG ACTTCATCAT 
GCAGGTTTTC AGGAAAGAGA TAGAAAAATT GTCGAAGAAT TATTTGTTAA CAATAAAATT 
CAGGTATTGA TTACAACAGC TACTTTAGCT TGGGGAGTTA ATTTCCCTGC ACACTTAGTT 
GTAATAAAAG GAACAGAATA TTATGATGGC AAACAACACC GTTATGTAGA CATGCCAATC 
ACAGATGTAC TACAAATGAT GGGTCGTGCA GGAAGACCCC AGTTTGATAA TTCAGGAGTG 
GCAGTAGTTT TAGTACACGA TTTAAAAAAA AATTATTATA AGAAATTTTT ACATCATCCA 
TTTCCAGTAG AATCAAGTTT ATTAGCTGTT CTGCCAGATC ATATAAATGC TGAAATTGTA 
GCTAATACAA TTAAAAATAA ACAAGAATTA TTAGATTATT TAACATGGAC ATATTTTTTT 
AGAAGGTTAA TGAAAAATCC TAGATATTAC AATTTAAGTA TTTTAGAACC TTATGCTATT 
AATCAATATC TCTCTTCTTT GGTTGATAAT ACACTAAAAG TATTAATAGA TTCATATTGT 
GTGGCATTTG ATGAGGAAGA ACAAATTTTA TATCCACTTT CAATGGGTAA AATAGCATCA 
TTTTATTACT TATCGCATCA TACTATGTTA ATGTTTGTAC AATCATTACA AGAATCTCTT 
ACATTGGAGC AATGTTTACA TATTTTATGT AATTCTTATG AATATAATGA ATTACCAGTA 
AGACACAATG AAGAATTATT GAATGAGGAA TTAAGTAAAA TGTGCCGTTA TCAAATAGAT 
AATTACTCAT ATAATTCTCC TCATACCAAA GCATTTTTAT TACTTCAAGC ACATTTCTCA 
AGACTTCCCT TACCATGTGT AGATTATTTT ACTGATTTAA AATCAGTTCT TGATCAGGCA 
ATTAGGATTA TACAAGCAAT GATAGATGCA GTTGCAGATC ATGGATGGTT AGCAAATACA 
ATTATGATAA TGAACATACT TCAAATGATT ATTCAAGCTC GATGGATTGA TGAATCAGCA 
ATTATTACAT TGCCATGTGT AAATTCAGAA CATTTAGAAT TATTTTCAAC ATTATCGCTT 
ACTCTTCCTG AATTATGTTT CAATATGCAC AACAAAGATA TTAAAATACT TAGAAAAGTA 
CTTAATAAAA GTTTTTCGCA AGAACAAATA TATCAGATAT ATCAAGTAAT AAAAGAAATG 
CCAATGCTCT GTATTAAGTT AAGCTTAGAA AGTTATGATG AAGATAATGA TAATAATAAA 
CAAAAAAATC AAATATTTAT ACCTCTAAAA TCTGATAATT TGGATTATAT AAATATTCAC 
AAGGATCAAG ATTACATATT AAATATTATA ATGAAAAGAA AAAATAAATC TAATAATTTA 
AAAGCACACT GTCCTTTGTT TCAAAAAGGA AAGGATGAAG GTTGGTTTTT AATTTTAGGT 
AATGTTTCTG ATAAAGAATT ATTAGTTCTA AAACGAGCAT CTGCAATAAA TGAACAACGG 
AAATATCAAT TACAATTCAC AGCTCCATCA AAATTAGGAC AAACAACTTT AACGTTTTAT 
TTGATTTCCG ATTGTTACAT AGGACTGGAT CAACAGTATG ATATAAAAAT AAATGTAATA 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552814 ( XM_026443880.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026299665.1 Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X1, mRNA.

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CloneID	OHd25552
Clone ID Related Accession (Same CDS sequence)	XM_026443881.1 , XM_026443880.1 , XM_026443882.1
Accession Version	XM_026443880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	activating signal cointegrator 1 complex subunit 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037648.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGAAT TACCTCGAAT TACAAGATCG TTACGAATGT TCACGAATCT TGGACGTATA 
AAAAGTACAA AGGAACTAGA ACCAAATGAT TTATTTAGAA AAGAAAAAGA GCAACGTAGC 
AAATCTAAAA GTGAATTCAA TTGGCAAGAT TTATGTAAAC ATGTATCTAA AAATGTTTTA 
TTTCATCTTA ATAAACTACG CCAAATTGCT ACAGAAGCAC TTGGGGAATG TTCTTTTGAA 
CTTATAAATG AATTTTTAAT GTTTACTTTA CAATTATTAA TTGATGACAA GTTGTTAACT 
CAAATAAAAT TTAAAATTTT AAAAGAAAAA GCAGGAAATT TAAAATTCCA TGATGCTAAC 
CACATGATGA AGATAATTAC AGAAATTCGA GAAAAATTAA ACGAAGAAAC TTTAATACTT 
ATAAAAAACG GAAAAGAAAA TATTAAAGTA TTGGAAAATG GCTTATTTGA TCCAAAATTT 
GAATATAAAT CTGCAAGAGT ATTATGGCCT GATACTAATA CACTTCGTAG TATGTCTGTG 
GGCAACATTA ATCAAAGTAC AGATAATTTC TCAATGAAAT ATAAAAATGA AGCTACAAAA 
TTATTTAAAA CAGAATTTGA CAAGACAGCA TATAAGGAAA ACTTGAAATC ATATCATACA 
AAGAATTTTG CAGATATATC TTTAGAAAAA TTTGAGAAGA TCATAATTAC TAAATTAGGT 
TCTAAAAATT CAGAGAACGA ATTAGTTGAT TTATTAGGTT GTGAATCTAT AGAATTTATT 
CAATATATTG TTCAACATCA GAAAAGTATT ATAACTTTAA GTTCTACTCC AAAAATACAA 
AAAAAATGTA ATATTCAAGG ACCTGTGATC AGTGGCCAAG TAACAGTTCA ATCTGAGAAA 
GAAAAACAAT TACAAAAACA AATACGGAAA CACGAAAAAA AATTAAATAA AACGAGTAAA 
CGAGAAGGAA AGGGAGAGTT GGAAGAAAAT GGCTTTGAAC CATTAGAATT GACTTTAAAA 
AAAAATGAAG CATTAATAGA AATGCACACA CCTATATTTA AAAAAAGTAT TAATACAGAT 
CGAGAAAAAT TTCCTTTTGT GTTTGATGCT AATATGAACA GTAGAAATAC AACTGGTTAT 
GTATCAAAAC AAAAAATAAT GTTACCGCAA AATGTTATAA GGAAAGATAC AGAAACATTT 
GAAGAAATTT TTATACCTAT TCCAGAATCT CAACCACTTG ATGTAGATTA TAAACCTATT 
ATGGTATCTT CATTGGATGA TATTGGACAA ATGGCTTTTA ATGGCATTGA ATCATTAAAT 
CGAATACAAA GTATTGTGTT CGATGTAGCA TATCATACTA ATGAGAATTT ATTAATTTGT 
GCTCCAACAG GAGCTGGAAA GACTAATGTT GCTATGTTAA CTATAGTACA TCAATTAAAG 
CAACATATTG AATATGGTCA ATTAATGAAA AATCAGTTCA AAATAATTTA TGTAACTCCA 
ATGAAAGCTT TAGCAGCTGA AATGACAGCA AATTTTAGCA AAAAACTTCA TTGTCTTGGT 
ATATCTGTTC GCGAGTTAAC TGGAGATATG CAATTAACCA AATCGGAAAT TCAACAAACA 
CAGATGATTG TTACCACACC AGAAAAGTGG GATGTTGTTA CTAGAAAAGG TACTGGAGAT 
ATTTCTCTCA CAAGTATTGT AAAACTGTTA ATTATTGATG AAGTACATTT GTTACATGGT 
GATAGAGGAC CTGTGGTTGA AGCCTTAGTT GCTCGAACTT TGAGACAAGT AGAATCTTCT 
CAAAGTATGA TCAGAATTGT TGGTCTTTCT GCAACATTGC CAAATTATGT AGATGTTGCA 
AGATTTTTAA GAGTGAATTC AAACATAGGA CTTTTTTATT TTGATCATCG ATTTCGACCA 
GTACCACTTA GTCAAGCGTT TATTGGTGTA AAAGCATCAT CATCATTACA AGAAATGAAT 
TACATGGATA ATGTATGTTA CAATAATGTT ATAGATATGG TACGTCAAGG ATATCAAGTA 
ATGGTATTTG TACATGCACG AAATGCTACT GTTAGAATGG CAAATACACT AAAAGATTTA 
GCAATAAAAC ATGGTACACT TAAATTATTT ATACCTGAAG GTCAAACAAA ATTTATAAAT 
AAAGCTTTTG CTAAATCACG TACGAAGTAT TTAAGTGAAT TATTTAATAA TGGATTATCA 
GTACATCATG CTGGTTTATT ACGTTCAGAG AGAAATTTAA TTGAAAAATA TTTTGCAGAT 
GGTTTAATTA AAGTATTAGT ATGTACGTCA ACATTAGCTT GGGGTGTAAA TCTACCTGCC 
CATGCTGTTA TTATAAGAGG AACAGAAATA TATGACGCAA AACATGGTTC TTATATTGAC 
TTAGATATAT TAGATGTTTT ACAAATTTTT GGTCGTGCTG GTAGACCACA ATTTGATGTA 
TCTGGTCATG CTGTTATTAT TACTTCCCAT AATAAATTAT ACCATTACTT ATCATTATTA 
ACAAATCAAA TACCTATAGA AAGCAATTTC ATTAAATATT TAGCTGATAA TTTAAATGCT 
GAAATTGCAT TGGGTACAAT ATCGAATGTA GAAGAAGCAA TAAAATGGTT AAGTTATACT 
TATTTATTCG TTCGAATGAA ATTAAATTTT CATTTTTATG GAATTCCTTA TCGAGTTATT 
TTGGAAGATC AAAATTTAGA ACAAAAAAGA AGAGAACTAA TTGATCAAGC AGCTAAAGCA 
TTAGATGAAG CTAAAATGAT TCGATATAAT ATAAATACTG GAGATCTGAG CACAACAAAT 
TTAGGACGAA TTGCGAGCCA TTTTTATCTT AAATATGACA CTATTAAAAT TTTTAATACA 
CTTCTAAAAC CAATTATGAC TGAAGCAGAA ATATTTTCTA TGATATCATA TTCTCAAGAA 
TTTGAACAAT TAAAAGTGCG AGATGATGAA ATGGAGGAAT TACAATATTT ATTTGAAGAA 
TATTGTAATA TTACAGTCCA AGGTGGTGTT GAAAATATAT ATGGTAAAGT TAATATATTA 
TTACAAACGT ATTTGTCACA TGGTAGAGTT AATGCGTTTT CATTAATATC TGATCAAGCA 
TATATTGTAC AAAATGCAGG ACGGATATTT CGAGCGTTGT TTGAAATTAT GTTAGGACAA 
AATAATGCAA TAATGGCTGG TCGTTTATTA GAAATAGCAA AAGTACTCGA AATACAACAA 
TGGAGTGATA GAAGTCCTCT ATGTCAATTT TCTTGTTTAT CTTTAGAAAT TATTGACAAG 
ATAGAACAAT ATGATTTAAC AGTTGAAAGA TTAAATAATA TGAATGTTAA AGAAATTGGA 
GATATTTTGC GTAATCAGAA AGAAGCAATA TTGGTGAAAA AATGTTGCAA AGAATTACCT 
GCATTAGAAA TGGAATCAAA TTTACAACCT ATTACTCGTA CAGTTTTAAG AATACATTTA 
AAAATATATC CTCAATTTCA TTGGAATGAT AATGTTCATG GTAAAACTTC AGAACCATTC 
TGGATATGGA TAGAAGATCC AGATAATAAT TTTATTTATC ATCATGAATA TTTTACTATG 
ACAAGAAAAA TGGTTTACAA TAATCTTGCA CAAGAACTTG TGATGACAAT ACCATTGCAT 
GAACCATTGC CTACTCAATA CATAGTAAGA GCAATAAGTG ATCGCTGGTT GGGTTCTGAA 
TATATGTTAC CTTTAACATT TCATGATTTA ATTTTACCTG AAGTATATCC ACCATATACT 
GATTTGTTAG AAGTACAACC TCTGCCAATA AAAGCATTGA AAGAACCATT ATTTGAAAAA 
CTTTATAAAT TTTCTCATTT TAATCCAATA CAGACACAAA TTTTTCATTG TTTGTATCAT 
ACAGACAATA ACGTCCTATT AGGAGCTCCA ACTGGTTCAG GAAAAACAAT TGCTGCGGAA 
ATTGCAATGT TTAGAGTATT TAAACAATAT CCAACGCAAA AAATTGTATA TATTGCACCA 
TTGAAAGCTT TAGTAAGAGA ACGAATGAAA GATTGGAAAA TTAGATTTGA AGAACAATTA 
GGAAAAAAAG TAATAGAATT AACTGGAGAT GTATCACCAG ATATTAAAGT TATAGCAAAT 
GCAAATATTA TTATAACTAC TCCAGAAAAA TGGGATGGTA TTAGTAGAAG TTGGCAAACA 
AGACTTTATG TCAAAAATGT TGCGCTTATT ATTATAGATG AAATACATTT ATTAGGGGAA 
GATCGTGGAC CAGTATTAGA AGTTATTATT TCAAGAACTA ATTTTATTTC TAGTCATACT 
TTAAAAAAAT TAAGAGTTAT TGGACTTTCT ACAGCATTAG CTAATGCAGT AGATTTAGCT 
AATTGGCTTG GTATTAAAGA GATGGGTCTT TATAATTTTC GTCCATCTGT ACGACCTGTA 
CCATTAGATA TTCATATAAC TGGATTCCCA GGGAAAAATT ATTGTCCTCG AATGGCAACA 
ATGAATAGAC CAACTTTTCA AGCAATTAAA CAACATGCTC CAACTAGTCC GGCTTTAGTA 
TTTGTTTCAT CACGTAGACA AACACGTTTA ACTGCATTAG ATTTGATTGC ATATCTTGCA 
GGAGAAGATA ATCCTAAGCA ATGGTTACAT ATGAGAGAAG AAGAAATGAA TAACATTCTA 
AGTCATATAC GAGATTCAAA TTTGAAACTT ACACTTGCTT TTGGAATTGG ACTTCATCAT 
GCAGGTTTTC AGGAAAGAGA TAGAAAAATT GTCGAAGAAT TATTTGTTAA CAATAAAATT 
CAGGTATTGA TTACAACAGC TACTTTAGCT TGGGGAGTTA ATTTCCCTGC ACACTTAGTT 
GTAATAAAAG GAACAGAATA TTATGATGGC AAACAACACC GTTATGTAGA CATGCCAATC 
ACAGATGTAC TACAAATGAT GGGTCGTGCA GGAAGACCCC AGTTTGATAA TTCAGGAGTG 
GCAGTAGTTT TAGTACACGA TTTAAAAAAA AATTATTATA AGAAATTTTT ACATCATCCA 
TTTCCAGTAG AATCAAGTTT ATTAGCTGTT CTGCCAGATC ATATAAATGC TGAAATTGTA 
GCTAATACAA TTAAAAATAA ACAAGAATTA TTAGATTATT TAACATGGAC ATATTTTTTT 
AGAAGGTTAA TGAAAAATCC TAGATATTAC AATTTAAGTA TTTTAGAACC TTATGCTATT 
AATCAATATC TCTCTTCTTT GGTTGATAAT ACACTAAAAG TATTAATAGA TTCATATTGT 
GTGGCATTTG ATGAGGAAGA ACAAATTTTA TATCCACTTT CAATGGGTAA AATAGCATCA 
TTTTATTACT TATCGCATCA TACTATGTTA ATGTTTGTAC AATCATTACA AGAATCTCTT 
ACATTGGAGC AATGTTTACA TATTTTATGT AATTCTTATG AATATAATGA ATTACCAGTA 
AGACACAATG AAGAATTATT GAATGAGGAA TTAAGTAAAA TGTGCCGTTA TCAAATAGAT 
AATTACTCAT ATAATTCTCC TCATACCAAA GCATTTTTAT TACTTCAAGC ACATTTCTCA 
AGACTTCCCT TACCATGTGT AGATTATTTT ACTGATTTAA AATCAGTTCT TGATCAGGCA 
ATTAGGATTA TACAAGCAAT GATAGATGCA GTTGCAGATC ATGGATGGTT AGCAAATACA 
ATTATGATAA TGAACATACT TCAAATGATT ATTCAAGCTC GATGGATTGA TGAATCAGCA 
ATTATTACAT TGCCATGTGT AAATTCAGAA CATTTAGAAT TATTTTCAAC ATTATCGCTT 
ACTCTTCCTG AATTATGTTT CAATATGCAC AACAAAGATA TTAAAATACT TAGAAAAGTA 
CTTAATAAAA GTTTTTCGCA AGAACAAATA TATCAGATAT ATCAAGTAAT AAAAGAAATG 
CCAATGCTCT GTATTAAGTT AAGCTTAGAA AGTTATGATG AAGATAATGA TAATAATAAA 
CAAAAAAATC AAATATTTAT ACCTCTAAAA TCTGATAATT TGGATTATAT AAATATTCAC 
AAGGATCAAG ATTACATATT AAATATTATA ATGAAAAGAA AAAATAAATC TAATAATTTA 
AAAGCACACT GTCCTTTGTT TCAAAAAGGA AAGGATGAAG GTTGGTTTTT AATTTTAGGT 
AATGTTTCTG ATAAAGAATT ATTAGTTCTA AAACGAGCAT CTGCAATAAA TGAACAACGG 
AAATATCAAT TACAATTCAC AGCTCCATCA AAATTAGGAC AAACAACTTT AACGTTTTAT 
TTGATTTCCG ATTGTTACAT AGGACTGGAT CAACAGTATG ATATAAAAAT AAATGTAATA 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026299665.1
CDS	271..6636
Translation	MPELPRITRSLRMFTNLGRIKSTKELEPNDLFRKEKEQRSKSKSEFNWQDLCKHVSKNVLFHLNKLRQIATEALGECSFELINEFLMFTLQLLIDDKLLTQIKFKILKEKAGNLKFHDANHMMKIITEIREKLNEETLILIKNGKENIKVLENGLFDPKFEYKSARVLWPDTNTLRSMSVGNINQSTDNFSMKYKNEATKLFKTEFDKTAYKENLKSYHTKNFADISLEKFEKIIITKLGSKNSENELVDLLGCESIEFIQYIVQHQKSIITLSSTPKIQKKCNIQGPVISGQVTVQSEKEKQLQKQIRKHEKKLNKTSKREGKGELEENGFEPLELTLKKNEALIEMHTPIFKKSINTDREKFPFVFDANMNSRNTTGYVSKQKIMLPQNVIRKDTETFEEIFIPIPESQPLDVDYKPIMVSSLDDIGQMAFNGIESLNRIQSIVFDVAYHTNENLLICAPTGAGKTNVAMLTIVHQLKQHIEYGQLMKNQFKIIYVTPMKALAAEMTANFSKKLHCLGISVRELTGDMQLTKSEIQQTQMIVTTPEKWDVVTRKGTGDISLTSIVKLLIIDEVHLLHGDRGPVVEALVARTLRQVESSQSMIRIVGLSATLPNYVDVARFLRVNSNIGLFYFDHRFRPVPLSQAFIGVKASSSLQEMNYMDNVCYNNVIDMVRQGYQVMVFVHARNATVRMANTLKDLAIKHGTLKLFIPEGQTKFINKAFAKSRTKYLSELFNNGLSVHHAGLLRSERNLIEKYFADGLIKVLVCTSTLAWGVNLPAHAVIIRGTEIYDAKHGSYIDLDILDVLQIFGRAGRPQFDVSGHAVIITSHNKLYHYLSLLTNQIPIESNFIKYLADNLNAEIALGTISNVEEAIKWLSYTYLFVRMKLNFHFYGIPYRVILEDQNLEQKRRELIDQAAKALDEAKMIRYNINTGDLSTTNLGRIASHFYLKYDTIKIFNTLLKPIMTEAEIFSMISYSQEFEQLKVRDDEMEELQYLFEEYCNITVQGGVENIYGKVNILLQTYLSHGRVNAFSLISDQAYIVQNAGRIFRALFEIMLGQNNAIMAGRLLEIAKVLEIQQWSDRSPLCQFSCLSLEIIDKIEQYDLTVERLNNMNVKEIGDILRNQKEAILVKKCCKELPALEMESNLQPITRTVLRIHLKIYPQFHWNDNVHGKTSEPFWIWIEDPDNNFIYHHEYFTMTRKMVYNNLAQELVMTIPLHEPLPTQYIVRAISDRWLGSEYMLPLTFHDLILPEVYPPYTDLLEVQPLPIKALKEPLFEKLYKFSHFNPIQTQIFHCLYHTDNNVLLGAPTGSGKTIAAEIAMFRVFKQYPTQKIVYIAPLKALVRERMKDWKIRFEEQLGKKVIELTGDVSPDIKVIANANIIITTPEKWDGISRSWQTRLYVKNVALIIIDEIHLLGEDRGPVLEVIISRTNFISSHTLKKLRVIGLSTALANAVDLANWLGIKEMGLYNFRPSVRPVPLDIHITGFPGKNYCPRMATMNRPTFQAIKQHAPTSPALVFVSSRRQTRLTALDLIAYLAGEDNPKQWLHMREEEMNNILSHIRDSNLKLTLAFGIGLHHAGFQERDRKIVEELFVNNKIQVLITTATLAWGVNFPAHLVVIKGTEYYDGKQHRYVDMPITDVLQMMGRAGRPQFDNSGVAVVLVHDLKKNYYKKFLHHPFPVESSLLAVLPDHINAEIVANTIKNKQELLDYLTWTYFFRRLMKNPRYYNLSILEPYAINQYLSSLVDNTLKVLIDSYCVAFDEEEQILYPLSMGKIASFYYLSHHTMLMFVQSLQESLTLEQCLHILCNSYEYNELPVRHNEELLNEELSKMCRYQIDNYSYNSPHTKAFLLLQAHFSRLPLPCVDYFTDLKSVLDQAIRIIQAMIDAVADHGWLANTIMIMNILQMIIQARWIDESAIITLPCVNSEHLELFSTLSLTLPELCFNMHNKDIKILRKVLNKSFSQEQIYQIYQVIKEMPMLCIKLSLESYDEDNDNNKQKNQIFIPLKSDNLDYINIHKDQDYILNIIMKRKNKSNNLKAHCPLFQKGKDEGWFLILGNVSDKELLVLKRASAINEQRKYQLQFTAPSKLGQTTLTFYLISDCYIGLDQQYDIKINVIS

Target ORF information:

RefSeq Version	XM_026443880.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026443880.1

ORF Insert Sequence:

ATGCCAGAAT TACCTCGAAT TACAAGATCG TTACGAATGT TCACGAATCT TGGACGTATA 
AAAAGTACAA AGGAACTAGA ACCAAATGAT TTATTTAGAA AAGAAAAAGA GCAACGTAGC 
AAATCTAAAA GTGAATTCAA TTGGCAAGAT TTATGTAAAC ATGTATCTAA AAATGTTTTA 
TTTCATCTTA ATAAACTACG CCAAATTGCT ACAGAAGCAC TTGGGGAATG TTCTTTTGAA 
CTTATAAATG AATTTTTAAT GTTTACTTTA CAATTATTAA TTGATGACAA GTTGTTAACT 
CAAATAAAAT TTAAAATTTT AAAAGAAAAA GCAGGAAATT TAAAATTCCA TGATGCTAAC 
CACATGATGA AGATAATTAC AGAAATTCGA GAAAAATTAA ACGAAGAAAC TTTAATACTT 
ATAAAAAACG GAAAAGAAAA TATTAAAGTA TTGGAAAATG GCTTATTTGA TCCAAAATTT 
GAATATAAAT CTGCAAGAGT ATTATGGCCT GATACTAATA CACTTCGTAG TATGTCTGTG 
GGCAACATTA ATCAAAGTAC AGATAATTTC TCAATGAAAT ATAAAAATGA AGCTACAAAA 
TTATTTAAAA CAGAATTTGA CAAGACAGCA TATAAGGAAA ACTTGAAATC ATATCATACA 
AAGAATTTTG CAGATATATC TTTAGAAAAA TTTGAGAAGA TCATAATTAC TAAATTAGGT 
TCTAAAAATT CAGAGAACGA ATTAGTTGAT TTATTAGGTT GTGAATCTAT AGAATTTATT 
CAATATATTG TTCAACATCA GAAAAGTATT ATAACTTTAA GTTCTACTCC AAAAATACAA 
AAAAAATGTA ATATTCAAGG ACCTGTGATC AGTGGCCAAG TAACAGTTCA ATCTGAGAAA 
GAAAAACAAT TACAAAAACA AATACGGAAA CACGAAAAAA AATTAAATAA AACGAGTAAA 
CGAGAAGGAA AGGGAGAGTT GGAAGAAAAT GGCTTTGAAC CATTAGAATT GACTTTAAAA 
AAAAATGAAG CATTAATAGA AATGCACACA CCTATATTTA AAAAAAGTAT TAATACAGAT 
CGAGAAAAAT TTCCTTTTGT GTTTGATGCT AATATGAACA GTAGAAATAC AACTGGTTAT 
GTATCAAAAC AAAAAATAAT GTTACCGCAA AATGTTATAA GGAAAGATAC AGAAACATTT 
GAAGAAATTT TTATACCTAT TCCAGAATCT CAACCACTTG ATGTAGATTA TAAACCTATT 
ATGGTATCTT CATTGGATGA TATTGGACAA ATGGCTTTTA ATGGCATTGA ATCATTAAAT 
CGAATACAAA GTATTGTGTT CGATGTAGCA TATCATACTA ATGAGAATTT ATTAATTTGT 
GCTCCAACAG GAGCTGGAAA GACTAATGTT GCTATGTTAA CTATAGTACA TCAATTAAAG 
CAACATATTG AATATGGTCA ATTAATGAAA AATCAGTTCA AAATAATTTA TGTAACTCCA 
ATGAAAGCTT TAGCAGCTGA AATGACAGCA AATTTTAGCA AAAAACTTCA TTGTCTTGGT 
ATATCTGTTC GCGAGTTAAC TGGAGATATG CAATTAACCA AATCGGAAAT TCAACAAACA 
CAGATGATTG TTACCACACC AGAAAAGTGG GATGTTGTTA CTAGAAAAGG TACTGGAGAT 
ATTTCTCTCA CAAGTATTGT AAAACTGTTA ATTATTGATG AAGTACATTT GTTACATGGT 
GATAGAGGAC CTGTGGTTGA AGCCTTAGTT GCTCGAACTT TGAGACAAGT AGAATCTTCT 
CAAAGTATGA TCAGAATTGT TGGTCTTTCT GCAACATTGC CAAATTATGT AGATGTTGCA 
AGATTTTTAA GAGTGAATTC AAACATAGGA CTTTTTTATT TTGATCATCG ATTTCGACCA 
GTACCACTTA GTCAAGCGTT TATTGGTGTA AAAGCATCAT CATCATTACA AGAAATGAAT 
TACATGGATA ATGTATGTTA CAATAATGTT ATAGATATGG TACGTCAAGG ATATCAAGTA 
ATGGTATTTG TACATGCACG AAATGCTACT GTTAGAATGG CAAATACACT AAAAGATTTA 
GCAATAAAAC ATGGTACACT TAAATTATTT ATACCTGAAG GTCAAACAAA ATTTATAAAT 
AAAGCTTTTG CTAAATCACG TACGAAGTAT TTAAGTGAAT TATTTAATAA TGGATTATCA 
GTACATCATG CTGGTTTATT ACGTTCAGAG AGAAATTTAA TTGAAAAATA TTTTGCAGAT 
GGTTTAATTA AAGTATTAGT ATGTACGTCA ACATTAGCTT GGGGTGTAAA TCTACCTGCC 
CATGCTGTTA TTATAAGAGG AACAGAAATA TATGACGCAA AACATGGTTC TTATATTGAC 
TTAGATATAT TAGATGTTTT ACAAATTTTT GGTCGTGCTG GTAGACCACA ATTTGATGTA 
TCTGGTCATG CTGTTATTAT TACTTCCCAT AATAAATTAT ACCATTACTT ATCATTATTA 
ACAAATCAAA TACCTATAGA AAGCAATTTC ATTAAATATT TAGCTGATAA TTTAAATGCT 
GAAATTGCAT TGGGTACAAT ATCGAATGTA GAAGAAGCAA TAAAATGGTT AAGTTATACT 
TATTTATTCG TTCGAATGAA ATTAAATTTT CATTTTTATG GAATTCCTTA TCGAGTTATT 
TTGGAAGATC AAAATTTAGA ACAAAAAAGA AGAGAACTAA TTGATCAAGC AGCTAAAGCA 
TTAGATGAAG CTAAAATGAT TCGATATAAT ATAAATACTG GAGATCTGAG CACAACAAAT 
TTAGGACGAA TTGCGAGCCA TTTTTATCTT AAATATGACA CTATTAAAAT TTTTAATACA 
CTTCTAAAAC CAATTATGAC TGAAGCAGAA ATATTTTCTA TGATATCATA TTCTCAAGAA 
TTTGAACAAT TAAAAGTGCG AGATGATGAA ATGGAGGAAT TACAATATTT ATTTGAAGAA 
TATTGTAATA TTACAGTCCA AGGTGGTGTT GAAAATATAT ATGGTAAAGT TAATATATTA 
TTACAAACGT ATTTGTCACA TGGTAGAGTT AATGCGTTTT CATTAATATC TGATCAAGCA 
TATATTGTAC AAAATGCAGG ACGGATATTT CGAGCGTTGT TTGAAATTAT GTTAGGACAA 
AATAATGCAA TAATGGCTGG TCGTTTATTA GAAATAGCAA AAGTACTCGA AATACAACAA 
TGGAGTGATA GAAGTCCTCT ATGTCAATTT TCTTGTTTAT CTTTAGAAAT TATTGACAAG 
ATAGAACAAT ATGATTTAAC AGTTGAAAGA TTAAATAATA TGAATGTTAA AGAAATTGGA 
GATATTTTGC GTAATCAGAA AGAAGCAATA TTGGTGAAAA AATGTTGCAA AGAATTACCT 
GCATTAGAAA TGGAATCAAA TTTACAACCT ATTACTCGTA CAGTTTTAAG AATACATTTA 
AAAATATATC CTCAATTTCA TTGGAATGAT AATGTTCATG GTAAAACTTC AGAACCATTC 
TGGATATGGA TAGAAGATCC AGATAATAAT TTTATTTATC ATCATGAATA TTTTACTATG 
ACAAGAAAAA TGGTTTACAA TAATCTTGCA CAAGAACTTG TGATGACAAT ACCATTGCAT 
GAACCATTGC CTACTCAATA CATAGTAAGA GCAATAAGTG ATCGCTGGTT GGGTTCTGAA 
TATATGTTAC CTTTAACATT TCATGATTTA ATTTTACCTG AAGTATATCC ACCATATACT 
GATTTGTTAG AAGTACAACC TCTGCCAATA AAAGCATTGA AAGAACCATT ATTTGAAAAA 
CTTTATAAAT TTTCTCATTT TAATCCAATA CAGACACAAA TTTTTCATTG TTTGTATCAT 
ACAGACAATA ACGTCCTATT AGGAGCTCCA ACTGGTTCAG GAAAAACAAT TGCTGCGGAA 
ATTGCAATGT TTAGAGTATT TAAACAATAT CCAACGCAAA AAATTGTATA TATTGCACCA 
TTGAAAGCTT TAGTAAGAGA ACGAATGAAA GATTGGAAAA TTAGATTTGA AGAACAATTA 
GGAAAAAAAG TAATAGAATT AACTGGAGAT GTATCACCAG ATATTAAAGT TATAGCAAAT 
GCAAATATTA TTATAACTAC TCCAGAAAAA TGGGATGGTA TTAGTAGAAG TTGGCAAACA 
AGACTTTATG TCAAAAATGT TGCGCTTATT ATTATAGATG AAATACATTT ATTAGGGGAA 
GATCGTGGAC CAGTATTAGA AGTTATTATT TCAAGAACTA ATTTTATTTC TAGTCATACT 
TTAAAAAAAT TAAGAGTTAT TGGACTTTCT ACAGCATTAG CTAATGCAGT AGATTTAGCT 
AATTGGCTTG GTATTAAAGA GATGGGTCTT TATAATTTTC GTCCATCTGT ACGACCTGTA 
CCATTAGATA TTCATATAAC TGGATTCCCA GGGAAAAATT ATTGTCCTCG AATGGCAACA 
ATGAATAGAC CAACTTTTCA AGCAATTAAA CAACATGCTC CAACTAGTCC GGCTTTAGTA 
TTTGTTTCAT CACGTAGACA AACACGTTTA ACTGCATTAG ATTTGATTGC ATATCTTGCA 
GGAGAAGATA ATCCTAAGCA ATGGTTACAT ATGAGAGAAG AAGAAATGAA TAACATTCTA 
AGTCATATAC GAGATTCAAA TTTGAAACTT ACACTTGCTT TTGGAATTGG ACTTCATCAT 
GCAGGTTTTC AGGAAAGAGA TAGAAAAATT GTCGAAGAAT TATTTGTTAA CAATAAAATT 
CAGGTATTGA TTACAACAGC TACTTTAGCT TGGGGAGTTA ATTTCCCTGC ACACTTAGTT 
GTAATAAAAG GAACAGAATA TTATGATGGC AAACAACACC GTTATGTAGA CATGCCAATC 
ACAGATGTAC TACAAATGAT GGGTCGTGCA GGAAGACCCC AGTTTGATAA TTCAGGAGTG 
GCAGTAGTTT TAGTACACGA TTTAAAAAAA AATTATTATA AGAAATTTTT ACATCATCCA 
TTTCCAGTAG AATCAAGTTT ATTAGCTGTT CTGCCAGATC ATATAAATGC TGAAATTGTA 
GCTAATACAA TTAAAAATAA ACAAGAATTA TTAGATTATT TAACATGGAC ATATTTTTTT 
AGAAGGTTAA TGAAAAATCC TAGATATTAC AATTTAAGTA TTTTAGAACC TTATGCTATT 
AATCAATATC TCTCTTCTTT GGTTGATAAT ACACTAAAAG TATTAATAGA TTCATATTGT 
GTGGCATTTG ATGAGGAAGA ACAAATTTTA TATCCACTTT CAATGGGTAA AATAGCATCA 
TTTTATTACT TATCGCATCA TACTATGTTA ATGTTTGTAC AATCATTACA AGAATCTCTT 
ACATTGGAGC AATGTTTACA TATTTTATGT AATTCTTATG AATATAATGA ATTACCAGTA 
AGACACAATG AAGAATTATT GAATGAGGAA TTAAGTAAAA TGTGCCGTTA TCAAATAGAT 
AATTACTCAT ATAATTCTCC TCATACCAAA GCATTTTTAT TACTTCAAGC ACATTTCTCA 
AGACTTCCCT TACCATGTGT AGATTATTTT ACTGATTTAA AATCAGTTCT TGATCAGGCA 
ATTAGGATTA TACAAGCAAT GATAGATGCA GTTGCAGATC ATGGATGGTT AGCAAATACA 
ATTATGATAA TGAACATACT TCAAATGATT ATTCAAGCTC GATGGATTGA TGAATCAGCA 
ATTATTACAT TGCCATGTGT AAATTCAGAA CATTTAGAAT TATTTTCAAC ATTATCGCTT 
ACTCTTCCTG AATTATGTTT CAATATGCAC AACAAAGATA TTAAAATACT TAGAAAAGTA 
CTTAATAAAA GTTTTTCGCA AGAACAAATA TATCAGATAT ATCAAGTAAT AAAAGAAATG 
CCAATGCTCT GTATTAAGTT AAGCTTAGAA AGTTATGATG AAGATAATGA TAATAATAAA 
CAAAAAAATC AAATATTTAT ACCTCTAAAA TCTGATAATT TGGATTATAT AAATATTCAC 
AAGGATCAAG ATTACATATT AAATATTATA ATGAAAAGAA AAAATAAATC TAATAATTTA 
AAAGCACACT GTCCTTTGTT TCAAAAAGGA AAGGATGAAG GTTGGTTTTT AATTTTAGGT 
AATGTTTCTG ATAAAGAATT ATTAGTTCTA AAACGAGCAT CTGCAATAAA TGAACAACGG 
AAATATCAAT TACAATTCAC AGCTCCATCA AAATTAGGAC AAACAACTTT AACGTTTTAT 
TTGATTTCCG ATTGTTACAT AGGACTGGAT CAACAGTATG ATATAAAAAT AAATGTAATA 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552814 ( XM_026443882.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026299667.1 Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X3, mRNA.

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CloneID	OHd25552
Clone ID Related Accession (Same CDS sequence)	XM_026443881.1 , XM_026443880.1 , XM_026443882.1
Accession Version	XM_026443882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	activating signal cointegrator 1 complex subunit 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037648.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGAAT TACCTCGAAT TACAAGATCG TTACGAATGT TCACGAATCT TGGACGTATA 
AAAAGTACAA AGGAACTAGA ACCAAATGAT TTATTTAGAA AAGAAAAAGA GCAACGTAGC 
AAATCTAAAA GTGAATTCAA TTGGCAAGAT TTATGTAAAC ATGTATCTAA AAATGTTTTA 
TTTCATCTTA ATAAACTACG CCAAATTGCT ACAGAAGCAC TTGGGGAATG TTCTTTTGAA 
CTTATAAATG AATTTTTAAT GTTTACTTTA CAATTATTAA TTGATGACAA GTTGTTAACT 
CAAATAAAAT TTAAAATTTT AAAAGAAAAA GCAGGAAATT TAAAATTCCA TGATGCTAAC 
CACATGATGA AGATAATTAC AGAAATTCGA GAAAAATTAA ACGAAGAAAC TTTAATACTT 
ATAAAAAACG GAAAAGAAAA TATTAAAGTA TTGGAAAATG GCTTATTTGA TCCAAAATTT 
GAATATAAAT CTGCAAGAGT ATTATGGCCT GATACTAATA CACTTCGTAG TATGTCTGTG 
GGCAACATTA ATCAAAGTAC AGATAATTTC TCAATGAAAT ATAAAAATGA AGCTACAAAA 
TTATTTAAAA CAGAATTTGA CAAGACAGCA TATAAGGAAA ACTTGAAATC ATATCATACA 
AAGAATTTTG CAGATATATC TTTAGAAAAA TTTGAGAAGA TCATAATTAC TAAATTAGGT 
TCTAAAAATT CAGAGAACGA ATTAGTTGAT TTATTAGGTT GTGAATCTAT AGAATTTATT 
CAATATATTG TTCAACATCA GAAAAGTATT ATAACTTTAA GTTCTACTCC AAAAATACAA 
AAAAAATGTA ATATTCAAGG ACCTGTGATC AGTGGCCAAG TAACAGTTCA ATCTGAGAAA 
GAAAAACAAT TACAAAAACA AATACGGAAA CACGAAAAAA AATTAAATAA AACGAGTAAA 
CGAGAAGGAA AGGGAGAGTT GGAAGAAAAT GGCTTTGAAC CATTAGAATT GACTTTAAAA 
AAAAATGAAG CATTAATAGA AATGCACACA CCTATATTTA AAAAAAGTAT TAATACAGAT 
CGAGAAAAAT TTCCTTTTGT GTTTGATGCT AATATGAACA GTAGAAATAC AACTGGTTAT 
GTATCAAAAC AAAAAATAAT GTTACCGCAA AATGTTATAA GGAAAGATAC AGAAACATTT 
GAAGAAATTT TTATACCTAT TCCAGAATCT CAACCACTTG ATGTAGATTA TAAACCTATT 
ATGGTATCTT CATTGGATGA TATTGGACAA ATGGCTTTTA ATGGCATTGA ATCATTAAAT 
CGAATACAAA GTATTGTGTT CGATGTAGCA TATCATACTA ATGAGAATTT ATTAATTTGT 
GCTCCAACAG GAGCTGGAAA GACTAATGTT GCTATGTTAA CTATAGTACA TCAATTAAAG 
CAACATATTG AATATGGTCA ATTAATGAAA AATCAGTTCA AAATAATTTA TGTAACTCCA 
ATGAAAGCTT TAGCAGCTGA AATGACAGCA AATTTTAGCA AAAAACTTCA TTGTCTTGGT 
ATATCTGTTC GCGAGTTAAC TGGAGATATG CAATTAACCA AATCGGAAAT TCAACAAACA 
CAGATGATTG TTACCACACC AGAAAAGTGG GATGTTGTTA CTAGAAAAGG TACTGGAGAT 
ATTTCTCTCA CAAGTATTGT AAAACTGTTA ATTATTGATG AAGTACATTT GTTACATGGT 
GATAGAGGAC CTGTGGTTGA AGCCTTAGTT GCTCGAACTT TGAGACAAGT AGAATCTTCT 
CAAAGTATGA TCAGAATTGT TGGTCTTTCT GCAACATTGC CAAATTATGT AGATGTTGCA 
AGATTTTTAA GAGTGAATTC AAACATAGGA CTTTTTTATT TTGATCATCG ATTTCGACCA 
GTACCACTTA GTCAAGCGTT TATTGGTGTA AAAGCATCAT CATCATTACA AGAAATGAAT 
TACATGGATA ATGTATGTTA CAATAATGTT ATAGATATGG TACGTCAAGG ATATCAAGTA 
ATGGTATTTG TACATGCACG AAATGCTACT GTTAGAATGG CAAATACACT AAAAGATTTA 
GCAATAAAAC ATGGTACACT TAAATTATTT ATACCTGAAG GTCAAACAAA ATTTATAAAT 
AAAGCTTTTG CTAAATCACG TACGAAGTAT TTAAGTGAAT TATTTAATAA TGGATTATCA 
GTACATCATG CTGGTTTATT ACGTTCAGAG AGAAATTTAA TTGAAAAATA TTTTGCAGAT 
GGTTTAATTA AAGTATTAGT ATGTACGTCA ACATTAGCTT GGGGTGTAAA TCTACCTGCC 
CATGCTGTTA TTATAAGAGG AACAGAAATA TATGACGCAA AACATGGTTC TTATATTGAC 
TTAGATATAT TAGATGTTTT ACAAATTTTT GGTCGTGCTG GTAGACCACA ATTTGATGTA 
TCTGGTCATG CTGTTATTAT TACTTCCCAT AATAAATTAT ACCATTACTT ATCATTATTA 
ACAAATCAAA TACCTATAGA AAGCAATTTC ATTAAATATT TAGCTGATAA TTTAAATGCT 
GAAATTGCAT TGGGTACAAT ATCGAATGTA GAAGAAGCAA TAAAATGGTT AAGTTATACT 
TATTTATTCG TTCGAATGAA ATTAAATTTT CATTTTTATG GAATTCCTTA TCGAGTTATT 
TTGGAAGATC AAAATTTAGA ACAAAAAAGA AGAGAACTAA TTGATCAAGC AGCTAAAGCA 
TTAGATGAAG CTAAAATGAT TCGATATAAT ATAAATACTG GAGATCTGAG CACAACAAAT 
TTAGGACGAA TTGCGAGCCA TTTTTATCTT AAATATGACA CTATTAAAAT TTTTAATACA 
CTTCTAAAAC CAATTATGAC TGAAGCAGAA ATATTTTCTA TGATATCATA TTCTCAAGAA 
TTTGAACAAT TAAAAGTGCG AGATGATGAA ATGGAGGAAT TACAATATTT ATTTGAAGAA 
TATTGTAATA TTACAGTCCA AGGTGGTGTT GAAAATATAT ATGGTAAAGT TAATATATTA 
TTACAAACGT ATTTGTCACA TGGTAGAGTT AATGCGTTTT CATTAATATC TGATCAAGCA 
TATATTGTAC AAAATGCAGG ACGGATATTT CGAGCGTTGT TTGAAATTAT GTTAGGACAA 
AATAATGCAA TAATGGCTGG TCGTTTATTA GAAATAGCAA AAGTACTCGA AATACAACAA 
TGGAGTGATA GAAGTCCTCT ATGTCAATTT TCTTGTTTAT CTTTAGAAAT TATTGACAAG 
ATAGAACAAT ATGATTTAAC AGTTGAAAGA TTAAATAATA TGAATGTTAA AGAAATTGGA 
GATATTTTGC GTAATCAGAA AGAAGCAATA TTGGTGAAAA AATGTTGCAA AGAATTACCT 
GCATTAGAAA TGGAATCAAA TTTACAACCT ATTACTCGTA CAGTTTTAAG AATACATTTA 
AAAATATATC CTCAATTTCA TTGGAATGAT AATGTTCATG GTAAAACTTC AGAACCATTC 
TGGATATGGA TAGAAGATCC AGATAATAAT TTTATTTATC ATCATGAATA TTTTACTATG 
ACAAGAAAAA TGGTTTACAA TAATCTTGCA CAAGAACTTG TGATGACAAT ACCATTGCAT 
GAACCATTGC CTACTCAATA CATAGTAAGA GCAATAAGTG ATCGCTGGTT GGGTTCTGAA 
TATATGTTAC CTTTAACATT TCATGATTTA ATTTTACCTG AAGTATATCC ACCATATACT 
GATTTGTTAG AAGTACAACC TCTGCCAATA AAAGCATTGA AAGAACCATT ATTTGAAAAA 
CTTTATAAAT TTTCTCATTT TAATCCAATA CAGACACAAA TTTTTCATTG TTTGTATCAT 
ACAGACAATA ACGTCCTATT AGGAGCTCCA ACTGGTTCAG GAAAAACAAT TGCTGCGGAA 
ATTGCAATGT TTAGAGTATT TAAACAATAT CCAACGCAAA AAATTGTATA TATTGCACCA 
TTGAAAGCTT TAGTAAGAGA ACGAATGAAA GATTGGAAAA TTAGATTTGA AGAACAATTA 
GGAAAAAAAG TAATAGAATT AACTGGAGAT GTATCACCAG ATATTAAAGT TATAGCAAAT 
GCAAATATTA TTATAACTAC TCCAGAAAAA TGGGATGGTA TTAGTAGAAG TTGGCAAACA 
AGACTTTATG TCAAAAATGT TGCGCTTATT ATTATAGATG AAATACATTT ATTAGGGGAA 
GATCGTGGAC CAGTATTAGA AGTTATTATT TCAAGAACTA ATTTTATTTC TAGTCATACT 
TTAAAAAAAT TAAGAGTTAT TGGACTTTCT ACAGCATTAG CTAATGCAGT AGATTTAGCT 
AATTGGCTTG GTATTAAAGA GATGGGTCTT TATAATTTTC GTCCATCTGT ACGACCTGTA 
CCATTAGATA TTCATATAAC TGGATTCCCA GGGAAAAATT ATTGTCCTCG AATGGCAACA 
ATGAATAGAC CAACTTTTCA AGCAATTAAA CAACATGCTC CAACTAGTCC GGCTTTAGTA 
TTTGTTTCAT CACGTAGACA AACACGTTTA ACTGCATTAG ATTTGATTGC ATATCTTGCA 
GGAGAAGATA ATCCTAAGCA ATGGTTACAT ATGAGAGAAG AAGAAATGAA TAACATTCTA 
AGTCATATAC GAGATTCAAA TTTGAAACTT ACACTTGCTT TTGGAATTGG ACTTCATCAT 
GCAGGTTTTC AGGAAAGAGA TAGAAAAATT GTCGAAGAAT TATTTGTTAA CAATAAAATT 
CAGGTATTGA TTACAACAGC TACTTTAGCT TGGGGAGTTA ATTTCCCTGC ACACTTAGTT 
GTAATAAAAG GAACAGAATA TTATGATGGC AAACAACACC GTTATGTAGA CATGCCAATC 
ACAGATGTAC TACAAATGAT GGGTCGTGCA GGAAGACCCC AGTTTGATAA TTCAGGAGTG 
GCAGTAGTTT TAGTACACGA TTTAAAAAAA AATTATTATA AGAAATTTTT ACATCATCCA 
TTTCCAGTAG AATCAAGTTT ATTAGCTGTT CTGCCAGATC ATATAAATGC TGAAATTGTA 
GCTAATACAA TTAAAAATAA ACAAGAATTA TTAGATTATT TAACATGGAC ATATTTTTTT 
AGAAGGTTAA TGAAAAATCC TAGATATTAC AATTTAAGTA TTTTAGAACC TTATGCTATT 
AATCAATATC TCTCTTCTTT GGTTGATAAT ACACTAAAAG TATTAATAGA TTCATATTGT 
GTGGCATTTG ATGAGGAAGA ACAAATTTTA TATCCACTTT CAATGGGTAA AATAGCATCA 
TTTTATTACT TATCGCATCA TACTATGTTA ATGTTTGTAC AATCATTACA AGAATCTCTT 
ACATTGGAGC AATGTTTACA TATTTTATGT AATTCTTATG AATATAATGA ATTACCAGTA 
AGACACAATG AAGAATTATT GAATGAGGAA TTAAGTAAAA TGTGCCGTTA TCAAATAGAT 
AATTACTCAT ATAATTCTCC TCATACCAAA GCATTTTTAT TACTTCAAGC ACATTTCTCA 
AGACTTCCCT TACCATGTGT AGATTATTTT ACTGATTTAA AATCAGTTCT TGATCAGGCA 
ATTAGGATTA TACAAGCAAT GATAGATGCA GTTGCAGATC ATGGATGGTT AGCAAATACA 
ATTATGATAA TGAACATACT TCAAATGATT ATTCAAGCTC GATGGATTGA TGAATCAGCA 
ATTATTACAT TGCCATGTGT AAATTCAGAA CATTTAGAAT TATTTTCAAC ATTATCGCTT 
ACTCTTCCTG AATTATGTTT CAATATGCAC AACAAAGATA TTAAAATACT TAGAAAAGTA 
CTTAATAAAA GTTTTTCGCA AGAACAAATA TATCAGATAT ATCAAGTAAT AAAAGAAATG 
CCAATGCTCT GTATTAAGTT AAGCTTAGAA AGTTATGATG AAGATAATGA TAATAATAAA 
CAAAAAAATC AAATATTTAT ACCTCTAAAA TCTGATAATT TGGATTATAT AAATATTCAC 
AAGGATCAAG ATTACATATT AAATATTATA ATGAAAAGAA AAAATAAATC TAATAATTTA 
AAAGCACACT GTCCTTTGTT TCAAAAAGGA AAGGATGAAG GTTGGTTTTT AATTTTAGGT 
AATGTTTCTG ATAAAGAATT ATTAGTTCTA AAACGAGCAT CTGCAATAAA TGAACAACGG 
AAATATCAAT TACAATTCAC AGCTCCATCA AAATTAGGAC AAACAACTTT AACGTTTTAT 
TTGATTTCCG ATTGTTACAT AGGACTGGAT CAACAGTATG ATATAAAAAT AAATGTAATA 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026299667.1
CDS	262..6627
Translation	MPELPRITRSLRMFTNLGRIKSTKELEPNDLFRKEKEQRSKSKSEFNWQDLCKHVSKNVLFHLNKLRQIATEALGECSFELINEFLMFTLQLLIDDKLLTQIKFKILKEKAGNLKFHDANHMMKIITEIREKLNEETLILIKNGKENIKVLENGLFDPKFEYKSARVLWPDTNTLRSMSVGNINQSTDNFSMKYKNEATKLFKTEFDKTAYKENLKSYHTKNFADISLEKFEKIIITKLGSKNSENELVDLLGCESIEFIQYIVQHQKSIITLSSTPKIQKKCNIQGPVISGQVTVQSEKEKQLQKQIRKHEKKLNKTSKREGKGELEENGFEPLELTLKKNEALIEMHTPIFKKSINTDREKFPFVFDANMNSRNTTGYVSKQKIMLPQNVIRKDTETFEEIFIPIPESQPLDVDYKPIMVSSLDDIGQMAFNGIESLNRIQSIVFDVAYHTNENLLICAPTGAGKTNVAMLTIVHQLKQHIEYGQLMKNQFKIIYVTPMKALAAEMTANFSKKLHCLGISVRELTGDMQLTKSEIQQTQMIVTTPEKWDVVTRKGTGDISLTSIVKLLIIDEVHLLHGDRGPVVEALVARTLRQVESSQSMIRIVGLSATLPNYVDVARFLRVNSNIGLFYFDHRFRPVPLSQAFIGVKASSSLQEMNYMDNVCYNNVIDMVRQGYQVMVFVHARNATVRMANTLKDLAIKHGTLKLFIPEGQTKFINKAFAKSRTKYLSELFNNGLSVHHAGLLRSERNLIEKYFADGLIKVLVCTSTLAWGVNLPAHAVIIRGTEIYDAKHGSYIDLDILDVLQIFGRAGRPQFDVSGHAVIITSHNKLYHYLSLLTNQIPIESNFIKYLADNLNAEIALGTISNVEEAIKWLSYTYLFVRMKLNFHFYGIPYRVILEDQNLEQKRRELIDQAAKALDEAKMIRYNINTGDLSTTNLGRIASHFYLKYDTIKIFNTLLKPIMTEAEIFSMISYSQEFEQLKVRDDEMEELQYLFEEYCNITVQGGVENIYGKVNILLQTYLSHGRVNAFSLISDQAYIVQNAGRIFRALFEIMLGQNNAIMAGRLLEIAKVLEIQQWSDRSPLCQFSCLSLEIIDKIEQYDLTVERLNNMNVKEIGDILRNQKEAILVKKCCKELPALEMESNLQPITRTVLRIHLKIYPQFHWNDNVHGKTSEPFWIWIEDPDNNFIYHHEYFTMTRKMVYNNLAQELVMTIPLHEPLPTQYIVRAISDRWLGSEYMLPLTFHDLILPEVYPPYTDLLEVQPLPIKALKEPLFEKLYKFSHFNPIQTQIFHCLYHTDNNVLLGAPTGSGKTIAAEIAMFRVFKQYPTQKIVYIAPLKALVRERMKDWKIRFEEQLGKKVIELTGDVSPDIKVIANANIIITTPEKWDGISRSWQTRLYVKNVALIIIDEIHLLGEDRGPVLEVIISRTNFISSHTLKKLRVIGLSTALANAVDLANWLGIKEMGLYNFRPSVRPVPLDIHITGFPGKNYCPRMATMNRPTFQAIKQHAPTSPALVFVSSRRQTRLTALDLIAYLAGEDNPKQWLHMREEEMNNILSHIRDSNLKLTLAFGIGLHHAGFQERDRKIVEELFVNNKIQVLITTATLAWGVNFPAHLVVIKGTEYYDGKQHRYVDMPITDVLQMMGRAGRPQFDNSGVAVVLVHDLKKNYYKKFLHHPFPVESSLLAVLPDHINAEIVANTIKNKQELLDYLTWTYFFRRLMKNPRYYNLSILEPYAINQYLSSLVDNTLKVLIDSYCVAFDEEEQILYPLSMGKIASFYYLSHHTMLMFVQSLQESLTLEQCLHILCNSYEYNELPVRHNEELLNEELSKMCRYQIDNYSYNSPHTKAFLLLQAHFSRLPLPCVDYFTDLKSVLDQAIRIIQAMIDAVADHGWLANTIMIMNILQMIIQARWIDESAIITLPCVNSEHLELFSTLSLTLPELCFNMHNKDIKILRKVLNKSFSQEQIYQIYQVIKEMPMLCIKLSLESYDEDNDNNKQKNQIFIPLKSDNLDYINIHKDQDYILNIIMKRKNKSNNLKAHCPLFQKGKDEGWFLILGNVSDKELLVLKRASAINEQRKYQLQFTAPSKLGQTTLTFYLISDCYIGLDQQYDIKINVIS

Target ORF information:

RefSeq Version	XM_026443882.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026443882.1

ORF Insert Sequence:

ATGCCAGAAT TACCTCGAAT TACAAGATCG TTACGAATGT TCACGAATCT TGGACGTATA 
AAAAGTACAA AGGAACTAGA ACCAAATGAT TTATTTAGAA AAGAAAAAGA GCAACGTAGC 
AAATCTAAAA GTGAATTCAA TTGGCAAGAT TTATGTAAAC ATGTATCTAA AAATGTTTTA 
TTTCATCTTA ATAAACTACG CCAAATTGCT ACAGAAGCAC TTGGGGAATG TTCTTTTGAA 
CTTATAAATG AATTTTTAAT GTTTACTTTA CAATTATTAA TTGATGACAA GTTGTTAACT 
CAAATAAAAT TTAAAATTTT AAAAGAAAAA GCAGGAAATT TAAAATTCCA TGATGCTAAC 
CACATGATGA AGATAATTAC AGAAATTCGA GAAAAATTAA ACGAAGAAAC TTTAATACTT 
ATAAAAAACG GAAAAGAAAA TATTAAAGTA TTGGAAAATG GCTTATTTGA TCCAAAATTT 
GAATATAAAT CTGCAAGAGT ATTATGGCCT GATACTAATA CACTTCGTAG TATGTCTGTG 
GGCAACATTA ATCAAAGTAC AGATAATTTC TCAATGAAAT ATAAAAATGA AGCTACAAAA 
TTATTTAAAA CAGAATTTGA CAAGACAGCA TATAAGGAAA ACTTGAAATC ATATCATACA 
AAGAATTTTG CAGATATATC TTTAGAAAAA TTTGAGAAGA TCATAATTAC TAAATTAGGT 
TCTAAAAATT CAGAGAACGA ATTAGTTGAT TTATTAGGTT GTGAATCTAT AGAATTTATT 
CAATATATTG TTCAACATCA GAAAAGTATT ATAACTTTAA GTTCTACTCC AAAAATACAA 
AAAAAATGTA ATATTCAAGG ACCTGTGATC AGTGGCCAAG TAACAGTTCA ATCTGAGAAA 
GAAAAACAAT TACAAAAACA AATACGGAAA CACGAAAAAA AATTAAATAA AACGAGTAAA 
CGAGAAGGAA AGGGAGAGTT GGAAGAAAAT GGCTTTGAAC CATTAGAATT GACTTTAAAA 
AAAAATGAAG CATTAATAGA AATGCACACA CCTATATTTA AAAAAAGTAT TAATACAGAT 
CGAGAAAAAT TTCCTTTTGT GTTTGATGCT AATATGAACA GTAGAAATAC AACTGGTTAT 
GTATCAAAAC AAAAAATAAT GTTACCGCAA AATGTTATAA GGAAAGATAC AGAAACATTT 
GAAGAAATTT TTATACCTAT TCCAGAATCT CAACCACTTG ATGTAGATTA TAAACCTATT 
ATGGTATCTT CATTGGATGA TATTGGACAA ATGGCTTTTA ATGGCATTGA ATCATTAAAT 
CGAATACAAA GTATTGTGTT CGATGTAGCA TATCATACTA ATGAGAATTT ATTAATTTGT 
GCTCCAACAG GAGCTGGAAA GACTAATGTT GCTATGTTAA CTATAGTACA TCAATTAAAG 
CAACATATTG AATATGGTCA ATTAATGAAA AATCAGTTCA AAATAATTTA TGTAACTCCA 
ATGAAAGCTT TAGCAGCTGA AATGACAGCA AATTTTAGCA AAAAACTTCA TTGTCTTGGT 
ATATCTGTTC GCGAGTTAAC TGGAGATATG CAATTAACCA AATCGGAAAT TCAACAAACA 
CAGATGATTG TTACCACACC AGAAAAGTGG GATGTTGTTA CTAGAAAAGG TACTGGAGAT 
ATTTCTCTCA CAAGTATTGT AAAACTGTTA ATTATTGATG AAGTACATTT GTTACATGGT 
GATAGAGGAC CTGTGGTTGA AGCCTTAGTT GCTCGAACTT TGAGACAAGT AGAATCTTCT 
CAAAGTATGA TCAGAATTGT TGGTCTTTCT GCAACATTGC CAAATTATGT AGATGTTGCA 
AGATTTTTAA GAGTGAATTC AAACATAGGA CTTTTTTATT TTGATCATCG ATTTCGACCA 
GTACCACTTA GTCAAGCGTT TATTGGTGTA AAAGCATCAT CATCATTACA AGAAATGAAT 
TACATGGATA ATGTATGTTA CAATAATGTT ATAGATATGG TACGTCAAGG ATATCAAGTA 
ATGGTATTTG TACATGCACG AAATGCTACT GTTAGAATGG CAAATACACT AAAAGATTTA 
GCAATAAAAC ATGGTACACT TAAATTATTT ATACCTGAAG GTCAAACAAA ATTTATAAAT 
AAAGCTTTTG CTAAATCACG TACGAAGTAT TTAAGTGAAT TATTTAATAA TGGATTATCA 
GTACATCATG CTGGTTTATT ACGTTCAGAG AGAAATTTAA TTGAAAAATA TTTTGCAGAT 
GGTTTAATTA AAGTATTAGT ATGTACGTCA ACATTAGCTT GGGGTGTAAA TCTACCTGCC 
CATGCTGTTA TTATAAGAGG AACAGAAATA TATGACGCAA AACATGGTTC TTATATTGAC 
TTAGATATAT TAGATGTTTT ACAAATTTTT GGTCGTGCTG GTAGACCACA ATTTGATGTA 
TCTGGTCATG CTGTTATTAT TACTTCCCAT AATAAATTAT ACCATTACTT ATCATTATTA 
ACAAATCAAA TACCTATAGA AAGCAATTTC ATTAAATATT TAGCTGATAA TTTAAATGCT 
GAAATTGCAT TGGGTACAAT ATCGAATGTA GAAGAAGCAA TAAAATGGTT AAGTTATACT 
TATTTATTCG TTCGAATGAA ATTAAATTTT CATTTTTATG GAATTCCTTA TCGAGTTATT 
TTGGAAGATC AAAATTTAGA ACAAAAAAGA AGAGAACTAA TTGATCAAGC AGCTAAAGCA 
TTAGATGAAG CTAAAATGAT TCGATATAAT ATAAATACTG GAGATCTGAG CACAACAAAT 
TTAGGACGAA TTGCGAGCCA TTTTTATCTT AAATATGACA CTATTAAAAT TTTTAATACA 
CTTCTAAAAC CAATTATGAC TGAAGCAGAA ATATTTTCTA TGATATCATA TTCTCAAGAA 
TTTGAACAAT TAAAAGTGCG AGATGATGAA ATGGAGGAAT TACAATATTT ATTTGAAGAA 
TATTGTAATA TTACAGTCCA AGGTGGTGTT GAAAATATAT ATGGTAAAGT TAATATATTA 
TTACAAACGT ATTTGTCACA TGGTAGAGTT AATGCGTTTT CATTAATATC TGATCAAGCA 
TATATTGTAC AAAATGCAGG ACGGATATTT CGAGCGTTGT TTGAAATTAT GTTAGGACAA 
AATAATGCAA TAATGGCTGG TCGTTTATTA GAAATAGCAA AAGTACTCGA AATACAACAA 
TGGAGTGATA GAAGTCCTCT ATGTCAATTT TCTTGTTTAT CTTTAGAAAT TATTGACAAG 
ATAGAACAAT ATGATTTAAC AGTTGAAAGA TTAAATAATA TGAATGTTAA AGAAATTGGA 
GATATTTTGC GTAATCAGAA AGAAGCAATA TTGGTGAAAA AATGTTGCAA AGAATTACCT 
GCATTAGAAA TGGAATCAAA TTTACAACCT ATTACTCGTA CAGTTTTAAG AATACATTTA 
AAAATATATC CTCAATTTCA TTGGAATGAT AATGTTCATG GTAAAACTTC AGAACCATTC 
TGGATATGGA TAGAAGATCC AGATAATAAT TTTATTTATC ATCATGAATA TTTTACTATG 
ACAAGAAAAA TGGTTTACAA TAATCTTGCA CAAGAACTTG TGATGACAAT ACCATTGCAT 
GAACCATTGC CTACTCAATA CATAGTAAGA GCAATAAGTG ATCGCTGGTT GGGTTCTGAA 
TATATGTTAC CTTTAACATT TCATGATTTA ATTTTACCTG AAGTATATCC ACCATATACT 
GATTTGTTAG AAGTACAACC TCTGCCAATA AAAGCATTGA AAGAACCATT ATTTGAAAAA 
CTTTATAAAT TTTCTCATTT TAATCCAATA CAGACACAAA TTTTTCATTG TTTGTATCAT 
ACAGACAATA ACGTCCTATT AGGAGCTCCA ACTGGTTCAG GAAAAACAAT TGCTGCGGAA 
ATTGCAATGT TTAGAGTATT TAAACAATAT CCAACGCAAA AAATTGTATA TATTGCACCA 
TTGAAAGCTT TAGTAAGAGA ACGAATGAAA GATTGGAAAA TTAGATTTGA AGAACAATTA 
GGAAAAAAAG TAATAGAATT AACTGGAGAT GTATCACCAG ATATTAAAGT TATAGCAAAT 
GCAAATATTA TTATAACTAC TCCAGAAAAA TGGGATGGTA TTAGTAGAAG TTGGCAAACA 
AGACTTTATG TCAAAAATGT TGCGCTTATT ATTATAGATG AAATACATTT ATTAGGGGAA 
GATCGTGGAC CAGTATTAGA AGTTATTATT TCAAGAACTA ATTTTATTTC TAGTCATACT 
TTAAAAAAAT TAAGAGTTAT TGGACTTTCT ACAGCATTAG CTAATGCAGT AGATTTAGCT 
AATTGGCTTG GTATTAAAGA GATGGGTCTT TATAATTTTC GTCCATCTGT ACGACCTGTA 
CCATTAGATA TTCATATAAC TGGATTCCCA GGGAAAAATT ATTGTCCTCG AATGGCAACA 
ATGAATAGAC CAACTTTTCA AGCAATTAAA CAACATGCTC CAACTAGTCC GGCTTTAGTA 
TTTGTTTCAT CACGTAGACA AACACGTTTA ACTGCATTAG ATTTGATTGC ATATCTTGCA 
GGAGAAGATA ATCCTAAGCA ATGGTTACAT ATGAGAGAAG AAGAAATGAA TAACATTCTA 
AGTCATATAC GAGATTCAAA TTTGAAACTT ACACTTGCTT TTGGAATTGG ACTTCATCAT 
GCAGGTTTTC AGGAAAGAGA TAGAAAAATT GTCGAAGAAT TATTTGTTAA CAATAAAATT 
CAGGTATTGA TTACAACAGC TACTTTAGCT TGGGGAGTTA ATTTCCCTGC ACACTTAGTT 
GTAATAAAAG GAACAGAATA TTATGATGGC AAACAACACC GTTATGTAGA CATGCCAATC 
ACAGATGTAC TACAAATGAT GGGTCGTGCA GGAAGACCCC AGTTTGATAA TTCAGGAGTG 
GCAGTAGTTT TAGTACACGA TTTAAAAAAA AATTATTATA AGAAATTTTT ACATCATCCA 
TTTCCAGTAG AATCAAGTTT ATTAGCTGTT CTGCCAGATC ATATAAATGC TGAAATTGTA 
GCTAATACAA TTAAAAATAA ACAAGAATTA TTAGATTATT TAACATGGAC ATATTTTTTT 
AGAAGGTTAA TGAAAAATCC TAGATATTAC AATTTAAGTA TTTTAGAACC TTATGCTATT 
AATCAATATC TCTCTTCTTT GGTTGATAAT ACACTAAAAG TATTAATAGA TTCATATTGT 
GTGGCATTTG ATGAGGAAGA ACAAATTTTA TATCCACTTT CAATGGGTAA AATAGCATCA 
TTTTATTACT TATCGCATCA TACTATGTTA ATGTTTGTAC AATCATTACA AGAATCTCTT 
ACATTGGAGC AATGTTTACA TATTTTATGT AATTCTTATG AATATAATGA ATTACCAGTA 
AGACACAATG AAGAATTATT GAATGAGGAA TTAAGTAAAA TGTGCCGTTA TCAAATAGAT 
AATTACTCAT ATAATTCTCC TCATACCAAA GCATTTTTAT TACTTCAAGC ACATTTCTCA 
AGACTTCCCT TACCATGTGT AGATTATTTT ACTGATTTAA AATCAGTTCT TGATCAGGCA 
ATTAGGATTA TACAAGCAAT GATAGATGCA GTTGCAGATC ATGGATGGTT AGCAAATACA 
ATTATGATAA TGAACATACT TCAAATGATT ATTCAAGCTC GATGGATTGA TGAATCAGCA 
ATTATTACAT TGCCATGTGT AAATTCAGAA CATTTAGAAT TATTTTCAAC ATTATCGCTT 
ACTCTTCCTG AATTATGTTT CAATATGCAC AACAAAGATA TTAAAATACT TAGAAAAGTA 
CTTAATAAAA GTTTTTCGCA AGAACAAATA TATCAGATAT ATCAAGTAAT AAAAGAAATG 
CCAATGCTCT GTATTAAGTT AAGCTTAGAA AGTTATGATG AAGATAATGA TAATAATAAA 
CAAAAAAATC AAATATTTAT ACCTCTAAAA TCTGATAATT TGGATTATAT AAATATTCAC 
AAGGATCAAG ATTACATATT AAATATTATA ATGAAAAGAA AAAATAAATC TAATAATTTA 
AAAGCACACT GTCCTTTGTT TCAAAAAGGA AAGGATGAAG GTTGGTTTTT AATTTTAGGT 
AATGTTTCTG ATAAAGAATT ATTAGTTCTA AAACGAGCAT CTGCAATAAA TGAACAACGG 
AAATATCAAT TACAATTCAC AGCTCCATCA AAATTAGGAC AAACAACTTT AACGTTTTAT 
TTGATTTCCG ATTGTTACAT AGGACTGGAT CAACAGTATG ATATAAAAAT AAATGTAATA 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552814 ( XM_026443884.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026299669.1 Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X5, mRNA.

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CloneID	OHd25553
Clone ID Related Accession (Same CDS sequence)	XM_026443884.1 , XM_026443885.1 , XM_026443883.1
Accession Version	XM_026443884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	activating signal cointegrator 1 complex subunit 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037648.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTATT TAGAAAAGAA AAAGAGCAAC GTAGCAAATC TAAAAGTGAA TTCAATTGGC 
AAGATTTATG TAAACATGTA TCTAAAAATG TTTTATTTCA TCTTAATAAA CTACGCCAAA 
TTGCTACAGA AGCACTTGAT AATTACAGAA ATTCGAGAAA AATTAAACGA AGAAACTTTA 
ATACTTATAA AAAACGGAAA AGAAAATATT AAAGTATTGG AAAATGGCTT ATTTGATCCA 
AAATTTGAAT ATAAATCTGC AAGAGTATTA TGGCCTGATA CTAATACACT TCGTAGTATG 
TCTGTGGGCA ACATTAATCA AAGTACAGAT AATTTCTCAA TGAAATATAA AAATGAAGCT 
ACAAAATTAT TTAAAACAGA ATTTGACAAG ACAGCATATA AGGAAAACTT GAAATCATAT 
CATACAAAGA ATTTTGCAGA TATATCTTTA GAAAAATTTG AGAAGATCAT AATTACTAAA 
TTAGGTTCTA AAAATTCAGA GAACGAATTA GTTGATTTAT TAGGTTGTGA ATCTATAGAA 
TTTATTCAAT ATATTGTTCA ACATCAGAAA AGTATTATAA CTTTAAGTTC TACTCCAAAA 
ATACAAAAAA AATGTAATAT TCAAGGACCT GTGATCAGTG GCCAAGTAAC AGTTCAATCT 
GAGAAAGAAA AACAATTACA AAAACAAATA CGGAAACACG AAAAAAAATT AAATAAAACG 
AGTAAACGAG AAGGAAAGGG AGAGTTGGAA GAAAATGGCT TTGAACCATT AGAATTGACT 
TTAAAAAAAA ATGAAGCATT AATAGAAATG CACACACCTA TATTTAAAAA AAGTATTAAT 
ACAGATCGAG AAAAATTTCC TTTTGTGTTT GATGCTAATA TGAACAGTAG AAATACAACT 
GGTTATGTAT CAAAACAAAA AATAATGTTA CCGCAAAATG TTATAAGGAA AGATACAGAA 
ACATTTGAAG AAATTTTTAT ACCTATTCCA GAATCTCAAC CACTTGATGT AGATTATAAA 
CCTATTATGG TATCTTCATT GGATGATATT GGACAAATGG CTTTTAATGG CATTGAATCA 
TTAAATCGAA TACAAAGTAT TGTGTTCGAT GTAGCATATC ATACTAATGA GAATTTATTA 
ATTTGTGCTC CAACAGGAGC TGGAAAGACT AATGTTGCTA TGTTAACTAT AGTACATCAA 
TTAAAGCAAC ATATTGAATA TGGTCAATTA ATGAAAAATC AGTTCAAAAT AATTTATGTA 
ACTCCAATGA AAGCTTTAGC AGCTGAAATG ACAGCAAATT TTAGCAAAAA ACTTCATTGT 
CTTGGTATAT CTGTTCGCGA GTTAACTGGA GATATGCAAT TAACCAAATC GGAAATTCAA 
CAAACACAGA TGATTGTTAC CACACCAGAA AAGTGGGATG TTGTTACTAG AAAAGGTACT 
GGAGATATTT CTCTCACAAG TATTGTAAAA CTGTTAATTA TTGATGAAGT ACATTTGTTA 
CATGGTGATA GAGGACCTGT GGTTGAAGCC TTAGTTGCTC GAACTTTGAG ACAAGTAGAA 
TCTTCTCAAA GTATGATCAG AATTGTTGGT CTTTCTGCAA CATTGCCAAA TTATGTAGAT 
GTTGCAAGAT TTTTAAGAGT GAATTCAAAC ATAGGACTTT TTTATTTTGA TCATCGATTT 
CGACCAGTAC CACTTAGTCA AGCGTTTATT GGTGTAAAAG CATCATCATC ATTACAAGAA 
ATGAATTACA TGGATAATGT ATGTTACAAT AATGTTATAG ATATGGTACG TCAAGGATAT 
CAAGTAATGG TATTTGTACA TGCACGAAAT GCTACTGTTA GAATGGCAAA TACACTAAAA 
GATTTAGCAA TAAAACATGG TACACTTAAA TTATTTATAC CTGAAGGTCA AACAAAATTT 
ATAAATAAAG CTTTTGCTAA ATCACGTACG AAGTATTTAA GTGAATTATT TAATAATGGA 
TTATCAGTAC ATCATGCTGG TTTATTACGT TCAGAGAGAA ATTTAATTGA AAAATATTTT 
GCAGATGGTT TAATTAAAGT ATTAGTATGT ACGTCAACAT TAGCTTGGGG TGTAAATCTA 
CCTGCCCATG CTGTTATTAT AAGAGGAACA GAAATATATG ACGCAAAACA TGGTTCTTAT 
ATTGACTTAG ATATATTAGA TGTTTTACAA ATTTTTGGTC GTGCTGGTAG ACCACAATTT 
GATGTATCTG GTCATGCTGT TATTATTACT TCCCATAATA AATTATACCA TTACTTATCA 
TTATTAACAA ATCAAATACC TATAGAAAGC AATTTCATTA AATATTTAGC TGATAATTTA 
AATGCTGAAA TTGCATTGGG TACAATATCG AATGTAGAAG AAGCAATAAA ATGGTTAAGT 
TATACTTATT TATTCGTTCG AATGAAATTA AATTTTCATT TTTATGGAAT TCCTTATCGA 
GTTATTTTGG AAGATCAAAA TTTAGAACAA AAAAGAAGAG AACTAATTGA TCAAGCAGCT 
AAAGCATTAG ATGAAGCTAA AATGATTCGA TATAATATAA ATACTGGAGA TCTGAGCACA 
ACAAATTTAG GACGAATTGC GAGCCATTTT TATCTTAAAT ATGACACTAT TAAAATTTTT 
AATACACTTC TAAAACCAAT TATGACTGAA GCAGAAATAT TTTCTATGAT ATCATATTCT 
CAAGAATTTG AACAATTAAA AGTGCGAGAT GATGAAATGG AGGAATTACA ATATTTATTT 
GAAGAATATT GTAATATTAC AGTCCAAGGT GGTGTTGAAA ATATATATGG TAAAGTTAAT 
ATATTATTAC AAACGTATTT GTCACATGGT AGAGTTAATG CGTTTTCATT AATATCTGAT 
CAAGCATATA TTGTACAAAA TGCAGGACGG ATATTTCGAG CGTTGTTTGA AATTATGTTA 
GGACAAAATA ATGCAATAAT GGCTGGTCGT TTATTAGAAA TAGCAAAAGT ACTCGAAATA 
CAACAATGGA GTGATAGAAG TCCTCTATGT CAATTTTCTT GTTTATCTTT AGAAATTATT 
GACAAGATAG AACAATATGA TTTAACAGTT GAAAGATTAA ATAATATGAA TGTTAAAGAA 
ATTGGAGATA TTTTGCGTAA TCAGAAAGAA GCAATATTGG TGAAAAAATG TTGCAAAGAA 
TTACCTGCAT TAGAAATGGA ATCAAATTTA CAACCTATTA CTCGTACAGT TTTAAGAATA 
CATTTAAAAA TATATCCTCA ATTTCATTGG AATGATAATG TTCATGGTAA AACTTCAGAA 
CCATTCTGGA TATGGATAGA AGATCCAGAT AATAATTTTA TTTATCATCA TGAATATTTT 
ACTATGACAA GAAAAATGGT TTACAATAAT CTTGCACAAG AACTTGTGAT GACAATACCA 
TTGCATGAAC CATTGCCTAC TCAATACATA GTAAGAGCAA TAAGTGATCG CTGGTTGGGT 
TCTGAATATA TGTTACCTTT AACATTTCAT GATTTAATTT TACCTGAAGT ATATCCACCA 
TATACTGATT TGTTAGAAGT ACAACCTCTG CCAATAAAAG CATTGAAAGA ACCATTATTT 
GAAAAACTTT ATAAATTTTC TCATTTTAAT CCAATACAGA CACAAATTTT TCATTGTTTG 
TATCATACAG ACAATAACGT CCTATTAGGA GCTCCAACTG GTTCAGGAAA AACAATTGCT 
GCGGAAATTG CAATGTTTAG AGTATTTAAA CAATATCCAA CGCAAAAAAT TGTATATATT 
GCACCATTGA AAGCTTTAGT AAGAGAACGA ATGAAAGATT GGAAAATTAG ATTTGAAGAA 
CAATTAGGAA AAAAAGTAAT AGAATTAACT GGAGATGTAT CACCAGATAT TAAAGTTATA 
GCAAATGCAA ATATTATTAT AACTACTCCA GAAAAATGGG ATGGTATTAG TAGAAGTTGG 
CAAACAAGAC TTTATGTCAA AAATGTTGCG CTTATTATTA TAGATGAAAT ACATTTATTA 
GGGGAAGATC GTGGACCAGT ATTAGAAGTT ATTATTTCAA GAACTAATTT TATTTCTAGT 
CATACTTTAA AAAAATTAAG AGTTATTGGA CTTTCTACAG CATTAGCTAA TGCAGTAGAT 
TTAGCTAATT GGCTTGGTAT TAAAGAGATG GGTCTTTATA ATTTTCGTCC ATCTGTACGA 
CCTGTACCAT TAGATATTCA TATAACTGGA TTCCCAGGGA AAAATTATTG TCCTCGAATG 
GCAACAATGA ATAGACCAAC TTTTCAAGCA ATTAAACAAC ATGCTCCAAC TAGTCCGGCT 
TTAGTATTTG TTTCATCACG TAGACAAACA CGTTTAACTG CATTAGATTT GATTGCATAT 
CTTGCAGGAG AAGATAATCC TAAGCAATGG TTACATATGA GAGAAGAAGA AATGAATAAC 
ATTCTAAGTC ATATACGAGA TTCAAATTTG AAACTTACAC TTGCTTTTGG AATTGGACTT 
CATCATGCAG GTTTTCAGGA AAGAGATAGA AAAATTGTCG AAGAATTATT TGTTAACAAT 
AAAATTCAGG TATTGATTAC AACAGCTACT TTAGCTTGGG GAGTTAATTT CCCTGCACAC 
TTAGTTGTAA TAAAAGGAAC AGAATATTAT GATGGCAAAC AACACCGTTA TGTAGACATG 
CCAATCACAG ATGTACTACA AATGATGGGT CGTGCAGGAA GACCCCAGTT TGATAATTCA 
GGAGTGGCAG TAGTTTTAGT ACACGATTTA AAAAAAAATT ATTATAAGAA ATTTTTACAT 
CATCCATTTC CAGTAGAATC AAGTTTATTA GCTGTTCTGC CAGATCATAT AAATGCTGAA 
ATTGTAGCTA ATACAATTAA AAATAAACAA GAATTATTAG ATTATTTAAC ATGGACATAT 
TTTTTTAGAA GGTTAATGAA AAATCCTAGA TATTACAATT TAAGTATTTT AGAACCTTAT 
GCTATTAATC AATATCTCTC TTCTTTGGTT GATAATACAC TAAAAGTATT AATAGATTCA 
TATTGTGTGG CATTTGATGA GGAAGAACAA ATTTTATATC CACTTTCAAT GGGTAAAATA 
GCATCATTTT ATTACTTATC GCATCATACT ATGTTAATGT TTGTACAATC ATTACAAGAA 
TCTCTTACAT TGGAGCAATG TTTACATATT TTATGTAATT CTTATGAATA TAATGAATTA 
CCAGTAAGAC ACAATGAAGA ATTATTGAAT GAGGAATTAA GTAAAATGTG CCGTTATCAA 
ATAGATAATT ACTCATATAA TTCTCCTCAT ACCAAAGCAT TTTTATTACT TCAAGCACAT 
TTCTCAAGAC TTCCCTTACC ATGTGTAGAT TATTTTACTG ATTTAAAATC AGTTCTTGAT 
CAGGCAATTA GGATTATACA AGCAATGATA GATGCAGTTG CAGATCATGG ATGGTTAGCA 
AATACAATTA TGATAATGAA CATACTTCAA ATGATTATTC AAGCTCGATG GATTGATGAA 
TCAGCAATTA TTACATTGCC ATGTGTAAAT TCAGAACATT TAGAATTATT TTCAACATTA 
TCGCTTACTC TTCCTGAATT ATGTTTCAAT ATGCACAACA AAGATATTAA AATACTTAGA 
AAAGTACTTA ATAAAAGTTT TTCGCAAGAA CAAATATATC AGATATATCA AGTAATAAAA 
GAAATGCCAA TGCTCTGTAT TAAGTTAAGC TTAGAAAGTT ATGATGAAGA TAATGATAAT 
AATAAACAAA AAAATCAAAT ATTTATACCT CTAAAATCTG ATAATTTGGA TTATATAAAT 
ATTCACAAGG ATCAAGATTA CATATTAAAT ATTATAATGA AAAGAAAAAA TAAATCTAAT 
AATTTAAAAG CACACTGTCC TTTGTTTCAA AAAGGAAAGG ATGAAGGTTG GTTTTTAATT 
TTAGGTAATG TTTCTGATAA AGAATTATTA GTTCTAAAAC GAGCATCTGC AATAAATGAA 
CAACGGAAAT ATCAATTACA ATTCACAGCT CCATCAAAAT TAGGACAAAC AACTTTAACG 
TTTTATTTGA TTTCCGATTG TTACATAGGA CTGGATCAAC AGTATGATAT AAAAATAAAT 
GTAATATCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026299669.1
CDS	383..6514
Translation	MIYLEKKKSNVANLKVNSIGKIYVNMYLKMFYFILINYAKLLQKHLIITEIREKLNEETLILIKNGKENIKVLENGLFDPKFEYKSARVLWPDTNTLRSMSVGNINQSTDNFSMKYKNEATKLFKTEFDKTAYKENLKSYHTKNFADISLEKFEKIIITKLGSKNSENELVDLLGCESIEFIQYIVQHQKSIITLSSTPKIQKKCNIQGPVISGQVTVQSEKEKQLQKQIRKHEKKLNKTSKREGKGELEENGFEPLELTLKKNEALIEMHTPIFKKSINTDREKFPFVFDANMNSRNTTGYVSKQKIMLPQNVIRKDTETFEEIFIPIPESQPLDVDYKPIMVSSLDDIGQMAFNGIESLNRIQSIVFDVAYHTNENLLICAPTGAGKTNVAMLTIVHQLKQHIEYGQLMKNQFKIIYVTPMKALAAEMTANFSKKLHCLGISVRELTGDMQLTKSEIQQTQMIVTTPEKWDVVTRKGTGDISLTSIVKLLIIDEVHLLHGDRGPVVEALVARTLRQVESSQSMIRIVGLSATLPNYVDVARFLRVNSNIGLFYFDHRFRPVPLSQAFIGVKASSSLQEMNYMDNVCYNNVIDMVRQGYQVMVFVHARNATVRMANTLKDLAIKHGTLKLFIPEGQTKFINKAFAKSRTKYLSELFNNGLSVHHAGLLRSERNLIEKYFADGLIKVLVCTSTLAWGVNLPAHAVIIRGTEIYDAKHGSYIDLDILDVLQIFGRAGRPQFDVSGHAVIITSHNKLYHYLSLLTNQIPIESNFIKYLADNLNAEIALGTISNVEEAIKWLSYTYLFVRMKLNFHFYGIPYRVILEDQNLEQKRRELIDQAAKALDEAKMIRYNINTGDLSTTNLGRIASHFYLKYDTIKIFNTLLKPIMTEAEIFSMISYSQEFEQLKVRDDEMEELQYLFEEYCNITVQGGVENIYGKVNILLQTYLSHGRVNAFSLISDQAYIVQNAGRIFRALFEIMLGQNNAIMAGRLLEIAKVLEIQQWSDRSPLCQFSCLSLEIIDKIEQYDLTVERLNNMNVKEIGDILRNQKEAILVKKCCKELPALEMESNLQPITRTVLRIHLKIYPQFHWNDNVHGKTSEPFWIWIEDPDNNFIYHHEYFTMTRKMVYNNLAQELVMTIPLHEPLPTQYIVRAISDRWLGSEYMLPLTFHDLILPEVYPPYTDLLEVQPLPIKALKEPLFEKLYKFSHFNPIQTQIFHCLYHTDNNVLLGAPTGSGKTIAAEIAMFRVFKQYPTQKIVYIAPLKALVRERMKDWKIRFEEQLGKKVIELTGDVSPDIKVIANANIIITTPEKWDGISRSWQTRLYVKNVALIIIDEIHLLGEDRGPVLEVIISRTNFISSHTLKKLRVIGLSTALANAVDLANWLGIKEMGLYNFRPSVRPVPLDIHITGFPGKNYCPRMATMNRPTFQAIKQHAPTSPALVFVSSRRQTRLTALDLIAYLAGEDNPKQWLHMREEEMNNILSHIRDSNLKLTLAFGIGLHHAGFQERDRKIVEELFVNNKIQVLITTATLAWGVNFPAHLVVIKGTEYYDGKQHRYVDMPITDVLQMMGRAGRPQFDNSGVAVVLVHDLKKNYYKKFLHHPFPVESSLLAVLPDHINAEIVANTIKNKQELLDYLTWTYFFRRLMKNPRYYNLSILEPYAINQYLSSLVDNTLKVLIDSYCVAFDEEEQILYPLSMGKIASFYYLSHHTMLMFVQSLQESLTLEQCLHILCNSYEYNELPVRHNEELLNEELSKMCRYQIDNYSYNSPHTKAFLLLQAHFSRLPLPCVDYFTDLKSVLDQAIRIIQAMIDAVADHGWLANTIMIMNILQMIIQARWIDESAIITLPCVNSEHLELFSTLSLTLPELCFNMHNKDIKILRKVLNKSFSQEQIYQIYQVIKEMPMLCIKLSLESYDEDNDNNKQKNQIFIPLKSDNLDYINIHKDQDYILNIIMKRKNKSNNLKAHCPLFQKGKDEGWFLILGNVSDKELLVLKRASAINEQRKYQLQFTAPSKLGQTTLTFYLISDCYIGLDQQYDIKINVIS

Target ORF information:

RefSeq Version	XM_026443884.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026443884.1

ORF Insert Sequence:

ATGATTTATT TAGAAAAGAA AAAGAGCAAC GTAGCAAATC TAAAAGTGAA TTCAATTGGC 
AAGATTTATG TAAACATGTA TCTAAAAATG TTTTATTTCA TCTTAATAAA CTACGCCAAA 
TTGCTACAGA AGCACTTGAT AATTACAGAA ATTCGAGAAA AATTAAACGA AGAAACTTTA 
ATACTTATAA AAAACGGAAA AGAAAATATT AAAGTATTGG AAAATGGCTT ATTTGATCCA 
AAATTTGAAT ATAAATCTGC AAGAGTATTA TGGCCTGATA CTAATACACT TCGTAGTATG 
TCTGTGGGCA ACATTAATCA AAGTACAGAT AATTTCTCAA TGAAATATAA AAATGAAGCT 
ACAAAATTAT TTAAAACAGA ATTTGACAAG ACAGCATATA AGGAAAACTT GAAATCATAT 
CATACAAAGA ATTTTGCAGA TATATCTTTA GAAAAATTTG AGAAGATCAT AATTACTAAA 
TTAGGTTCTA AAAATTCAGA GAACGAATTA GTTGATTTAT TAGGTTGTGA ATCTATAGAA 
TTTATTCAAT ATATTGTTCA ACATCAGAAA AGTATTATAA CTTTAAGTTC TACTCCAAAA 
ATACAAAAAA AATGTAATAT TCAAGGACCT GTGATCAGTG GCCAAGTAAC AGTTCAATCT 
GAGAAAGAAA AACAATTACA AAAACAAATA CGGAAACACG AAAAAAAATT AAATAAAACG 
AGTAAACGAG AAGGAAAGGG AGAGTTGGAA GAAAATGGCT TTGAACCATT AGAATTGACT 
TTAAAAAAAA ATGAAGCATT AATAGAAATG CACACACCTA TATTTAAAAA AAGTATTAAT 
ACAGATCGAG AAAAATTTCC TTTTGTGTTT GATGCTAATA TGAACAGTAG AAATACAACT 
GGTTATGTAT CAAAACAAAA AATAATGTTA CCGCAAAATG TTATAAGGAA AGATACAGAA 
ACATTTGAAG AAATTTTTAT ACCTATTCCA GAATCTCAAC CACTTGATGT AGATTATAAA 
CCTATTATGG TATCTTCATT GGATGATATT GGACAAATGG CTTTTAATGG CATTGAATCA 
TTAAATCGAA TACAAAGTAT TGTGTTCGAT GTAGCATATC ATACTAATGA GAATTTATTA 
ATTTGTGCTC CAACAGGAGC TGGAAAGACT AATGTTGCTA TGTTAACTAT AGTACATCAA 
TTAAAGCAAC ATATTGAATA TGGTCAATTA ATGAAAAATC AGTTCAAAAT AATTTATGTA 
ACTCCAATGA AAGCTTTAGC AGCTGAAATG ACAGCAAATT TTAGCAAAAA ACTTCATTGT 
CTTGGTATAT CTGTTCGCGA GTTAACTGGA GATATGCAAT TAACCAAATC GGAAATTCAA 
CAAACACAGA TGATTGTTAC CACACCAGAA AAGTGGGATG TTGTTACTAG AAAAGGTACT 
GGAGATATTT CTCTCACAAG TATTGTAAAA CTGTTAATTA TTGATGAAGT ACATTTGTTA 
CATGGTGATA GAGGACCTGT GGTTGAAGCC TTAGTTGCTC GAACTTTGAG ACAAGTAGAA 
TCTTCTCAAA GTATGATCAG AATTGTTGGT CTTTCTGCAA CATTGCCAAA TTATGTAGAT 
GTTGCAAGAT TTTTAAGAGT GAATTCAAAC ATAGGACTTT TTTATTTTGA TCATCGATTT 
CGACCAGTAC CACTTAGTCA AGCGTTTATT GGTGTAAAAG CATCATCATC ATTACAAGAA 
ATGAATTACA TGGATAATGT ATGTTACAAT AATGTTATAG ATATGGTACG TCAAGGATAT 
CAAGTAATGG TATTTGTACA TGCACGAAAT GCTACTGTTA GAATGGCAAA TACACTAAAA 
GATTTAGCAA TAAAACATGG TACACTTAAA TTATTTATAC CTGAAGGTCA AACAAAATTT 
ATAAATAAAG CTTTTGCTAA ATCACGTACG AAGTATTTAA GTGAATTATT TAATAATGGA 
TTATCAGTAC ATCATGCTGG TTTATTACGT TCAGAGAGAA ATTTAATTGA AAAATATTTT 
GCAGATGGTT TAATTAAAGT ATTAGTATGT ACGTCAACAT TAGCTTGGGG TGTAAATCTA 
CCTGCCCATG CTGTTATTAT AAGAGGAACA GAAATATATG ACGCAAAACA TGGTTCTTAT 
ATTGACTTAG ATATATTAGA TGTTTTACAA ATTTTTGGTC GTGCTGGTAG ACCACAATTT 
GATGTATCTG GTCATGCTGT TATTATTACT TCCCATAATA AATTATACCA TTACTTATCA 
TTATTAACAA ATCAAATACC TATAGAAAGC AATTTCATTA AATATTTAGC TGATAATTTA 
AATGCTGAAA TTGCATTGGG TACAATATCG AATGTAGAAG AAGCAATAAA ATGGTTAAGT 
TATACTTATT TATTCGTTCG AATGAAATTA AATTTTCATT TTTATGGAAT TCCTTATCGA 
GTTATTTTGG AAGATCAAAA TTTAGAACAA AAAAGAAGAG AACTAATTGA TCAAGCAGCT 
AAAGCATTAG ATGAAGCTAA AATGATTCGA TATAATATAA ATACTGGAGA TCTGAGCACA 
ACAAATTTAG GACGAATTGC GAGCCATTTT TATCTTAAAT ATGACACTAT TAAAATTTTT 
AATACACTTC TAAAACCAAT TATGACTGAA GCAGAAATAT TTTCTATGAT ATCATATTCT 
CAAGAATTTG AACAATTAAA AGTGCGAGAT GATGAAATGG AGGAATTACA ATATTTATTT 
GAAGAATATT GTAATATTAC AGTCCAAGGT GGTGTTGAAA ATATATATGG TAAAGTTAAT 
ATATTATTAC AAACGTATTT GTCACATGGT AGAGTTAATG CGTTTTCATT AATATCTGAT 
CAAGCATATA TTGTACAAAA TGCAGGACGG ATATTTCGAG CGTTGTTTGA AATTATGTTA 
GGACAAAATA ATGCAATAAT GGCTGGTCGT TTATTAGAAA TAGCAAAAGT ACTCGAAATA 
CAACAATGGA GTGATAGAAG TCCTCTATGT CAATTTTCTT GTTTATCTTT AGAAATTATT 
GACAAGATAG AACAATATGA TTTAACAGTT GAAAGATTAA ATAATATGAA TGTTAAAGAA 
ATTGGAGATA TTTTGCGTAA TCAGAAAGAA GCAATATTGG TGAAAAAATG TTGCAAAGAA 
TTACCTGCAT TAGAAATGGA ATCAAATTTA CAACCTATTA CTCGTACAGT TTTAAGAATA 
CATTTAAAAA TATATCCTCA ATTTCATTGG AATGATAATG TTCATGGTAA AACTTCAGAA 
CCATTCTGGA TATGGATAGA AGATCCAGAT AATAATTTTA TTTATCATCA TGAATATTTT 
ACTATGACAA GAAAAATGGT TTACAATAAT CTTGCACAAG AACTTGTGAT GACAATACCA 
TTGCATGAAC CATTGCCTAC TCAATACATA GTAAGAGCAA TAAGTGATCG CTGGTTGGGT 
TCTGAATATA TGTTACCTTT AACATTTCAT GATTTAATTT TACCTGAAGT ATATCCACCA 
TATACTGATT TGTTAGAAGT ACAACCTCTG CCAATAAAAG CATTGAAAGA ACCATTATTT 
GAAAAACTTT ATAAATTTTC TCATTTTAAT CCAATACAGA CACAAATTTT TCATTGTTTG 
TATCATACAG ACAATAACGT CCTATTAGGA GCTCCAACTG GTTCAGGAAA AACAATTGCT 
GCGGAAATTG CAATGTTTAG AGTATTTAAA CAATATCCAA CGCAAAAAAT TGTATATATT 
GCACCATTGA AAGCTTTAGT AAGAGAACGA ATGAAAGATT GGAAAATTAG ATTTGAAGAA 
CAATTAGGAA AAAAAGTAAT AGAATTAACT GGAGATGTAT CACCAGATAT TAAAGTTATA 
GCAAATGCAA ATATTATTAT AACTACTCCA GAAAAATGGG ATGGTATTAG TAGAAGTTGG 
CAAACAAGAC TTTATGTCAA AAATGTTGCG CTTATTATTA TAGATGAAAT ACATTTATTA 
GGGGAAGATC GTGGACCAGT ATTAGAAGTT ATTATTTCAA GAACTAATTT TATTTCTAGT 
CATACTTTAA AAAAATTAAG AGTTATTGGA CTTTCTACAG CATTAGCTAA TGCAGTAGAT 
TTAGCTAATT GGCTTGGTAT TAAAGAGATG GGTCTTTATA ATTTTCGTCC ATCTGTACGA 
CCTGTACCAT TAGATATTCA TATAACTGGA TTCCCAGGGA AAAATTATTG TCCTCGAATG 
GCAACAATGA ATAGACCAAC TTTTCAAGCA ATTAAACAAC ATGCTCCAAC TAGTCCGGCT 
TTAGTATTTG TTTCATCACG TAGACAAACA CGTTTAACTG CATTAGATTT GATTGCATAT 
CTTGCAGGAG AAGATAATCC TAAGCAATGG TTACATATGA GAGAAGAAGA AATGAATAAC 
ATTCTAAGTC ATATACGAGA TTCAAATTTG AAACTTACAC TTGCTTTTGG AATTGGACTT 
CATCATGCAG GTTTTCAGGA AAGAGATAGA AAAATTGTCG AAGAATTATT TGTTAACAAT 
AAAATTCAGG TATTGATTAC AACAGCTACT TTAGCTTGGG GAGTTAATTT CCCTGCACAC 
TTAGTTGTAA TAAAAGGAAC AGAATATTAT GATGGCAAAC AACACCGTTA TGTAGACATG 
CCAATCACAG ATGTACTACA AATGATGGGT CGTGCAGGAA GACCCCAGTT TGATAATTCA 
GGAGTGGCAG TAGTTTTAGT ACACGATTTA AAAAAAAATT ATTATAAGAA ATTTTTACAT 
CATCCATTTC CAGTAGAATC AAGTTTATTA GCTGTTCTGC CAGATCATAT AAATGCTGAA 
ATTGTAGCTA ATACAATTAA AAATAAACAA GAATTATTAG ATTATTTAAC ATGGACATAT 
TTTTTTAGAA GGTTAATGAA AAATCCTAGA TATTACAATT TAAGTATTTT AGAACCTTAT 
GCTATTAATC AATATCTCTC TTCTTTGGTT GATAATACAC TAAAAGTATT AATAGATTCA 
TATTGTGTGG CATTTGATGA GGAAGAACAA ATTTTATATC CACTTTCAAT GGGTAAAATA 
GCATCATTTT ATTACTTATC GCATCATACT ATGTTAATGT TTGTACAATC ATTACAAGAA 
TCTCTTACAT TGGAGCAATG TTTACATATT TTATGTAATT CTTATGAATA TAATGAATTA 
CCAGTAAGAC ACAATGAAGA ATTATTGAAT GAGGAATTAA GTAAAATGTG CCGTTATCAA 
ATAGATAATT ACTCATATAA TTCTCCTCAT ACCAAAGCAT TTTTATTACT TCAAGCACAT 
TTCTCAAGAC TTCCCTTACC ATGTGTAGAT TATTTTACTG ATTTAAAATC AGTTCTTGAT 
CAGGCAATTA GGATTATACA AGCAATGATA GATGCAGTTG CAGATCATGG ATGGTTAGCA 
AATACAATTA TGATAATGAA CATACTTCAA ATGATTATTC AAGCTCGATG GATTGATGAA 
TCAGCAATTA TTACATTGCC ATGTGTAAAT TCAGAACATT TAGAATTATT TTCAACATTA 
TCGCTTACTC TTCCTGAATT ATGTTTCAAT ATGCACAACA AAGATATTAA AATACTTAGA 
AAAGTACTTA ATAAAAGTTT TTCGCAAGAA CAAATATATC AGATATATCA AGTAATAAAA 
GAAATGCCAA TGCTCTGTAT TAAGTTAAGC TTAGAAAGTT ATGATGAAGA TAATGATAAT 
AATAAACAAA AAAATCAAAT ATTTATACCT CTAAAATCTG ATAATTTGGA TTATATAAAT 
ATTCACAAGG ATCAAGATTA CATATTAAAT ATTATAATGA AAAGAAAAAA TAAATCTAAT 
AATTTAAAAG CACACTGTCC TTTGTTTCAA AAAGGAAAGG ATGAAGGTTG GTTTTTAATT 
TTAGGTAATG TTTCTGATAA AGAATTATTA GTTCTAAAAC GAGCATCTGC AATAAATGAA 
CAACGGAAAT ATCAATTACA ATTCACAGCT CCATCAAAAT TAGGACAAAC AACTTTAACG 
TTTTATTTGA TTTCCGATTG TTACATAGGA CTGGATCAAC AGTATGATAT AAAAATAAAT 
GTAATATCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552814 ( XM_026443885.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026299670.1 Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X6, mRNA.

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CloneID	OHd25553
Clone ID Related Accession (Same CDS sequence)	XM_026443884.1 , XM_026443885.1 , XM_026443883.1
Accession Version	XM_026443885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	activating signal cointegrator 1 complex subunit 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037648.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTATT TAGAAAAGAA AAAGAGCAAC GTAGCAAATC TAAAAGTGAA TTCAATTGGC 
AAGATTTATG TAAACATGTA TCTAAAAATG TTTTATTTCA TCTTAATAAA CTACGCCAAA 
TTGCTACAGA AGCACTTGAT AATTACAGAA ATTCGAGAAA AATTAAACGA AGAAACTTTA 
ATACTTATAA AAAACGGAAA AGAAAATATT AAAGTATTGG AAAATGGCTT ATTTGATCCA 
AAATTTGAAT ATAAATCTGC AAGAGTATTA TGGCCTGATA CTAATACACT TCGTAGTATG 
TCTGTGGGCA ACATTAATCA AAGTACAGAT AATTTCTCAA TGAAATATAA AAATGAAGCT 
ACAAAATTAT TTAAAACAGA ATTTGACAAG ACAGCATATA AGGAAAACTT GAAATCATAT 
CATACAAAGA ATTTTGCAGA TATATCTTTA GAAAAATTTG AGAAGATCAT AATTACTAAA 
TTAGGTTCTA AAAATTCAGA GAACGAATTA GTTGATTTAT TAGGTTGTGA ATCTATAGAA 
TTTATTCAAT ATATTGTTCA ACATCAGAAA AGTATTATAA CTTTAAGTTC TACTCCAAAA 
ATACAAAAAA AATGTAATAT TCAAGGACCT GTGATCAGTG GCCAAGTAAC AGTTCAATCT 
GAGAAAGAAA AACAATTACA AAAACAAATA CGGAAACACG AAAAAAAATT AAATAAAACG 
AGTAAACGAG AAGGAAAGGG AGAGTTGGAA GAAAATGGCT TTGAACCATT AGAATTGACT 
TTAAAAAAAA ATGAAGCATT AATAGAAATG CACACACCTA TATTTAAAAA AAGTATTAAT 
ACAGATCGAG AAAAATTTCC TTTTGTGTTT GATGCTAATA TGAACAGTAG AAATACAACT 
GGTTATGTAT CAAAACAAAA AATAATGTTA CCGCAAAATG TTATAAGGAA AGATACAGAA 
ACATTTGAAG AAATTTTTAT ACCTATTCCA GAATCTCAAC CACTTGATGT AGATTATAAA 
CCTATTATGG TATCTTCATT GGATGATATT GGACAAATGG CTTTTAATGG CATTGAATCA 
TTAAATCGAA TACAAAGTAT TGTGTTCGAT GTAGCATATC ATACTAATGA GAATTTATTA 
ATTTGTGCTC CAACAGGAGC TGGAAAGACT AATGTTGCTA TGTTAACTAT AGTACATCAA 
TTAAAGCAAC ATATTGAATA TGGTCAATTA ATGAAAAATC AGTTCAAAAT AATTTATGTA 
ACTCCAATGA AAGCTTTAGC AGCTGAAATG ACAGCAAATT TTAGCAAAAA ACTTCATTGT 
CTTGGTATAT CTGTTCGCGA GTTAACTGGA GATATGCAAT TAACCAAATC GGAAATTCAA 
CAAACACAGA TGATTGTTAC CACACCAGAA AAGTGGGATG TTGTTACTAG AAAAGGTACT 
GGAGATATTT CTCTCACAAG TATTGTAAAA CTGTTAATTA TTGATGAAGT ACATTTGTTA 
CATGGTGATA GAGGACCTGT GGTTGAAGCC TTAGTTGCTC GAACTTTGAG ACAAGTAGAA 
TCTTCTCAAA GTATGATCAG AATTGTTGGT CTTTCTGCAA CATTGCCAAA TTATGTAGAT 
GTTGCAAGAT TTTTAAGAGT GAATTCAAAC ATAGGACTTT TTTATTTTGA TCATCGATTT 
CGACCAGTAC CACTTAGTCA AGCGTTTATT GGTGTAAAAG CATCATCATC ATTACAAGAA 
ATGAATTACA TGGATAATGT ATGTTACAAT AATGTTATAG ATATGGTACG TCAAGGATAT 
CAAGTAATGG TATTTGTACA TGCACGAAAT GCTACTGTTA GAATGGCAAA TACACTAAAA 
GATTTAGCAA TAAAACATGG TACACTTAAA TTATTTATAC CTGAAGGTCA AACAAAATTT 
ATAAATAAAG CTTTTGCTAA ATCACGTACG AAGTATTTAA GTGAATTATT TAATAATGGA 
TTATCAGTAC ATCATGCTGG TTTATTACGT TCAGAGAGAA ATTTAATTGA AAAATATTTT 
GCAGATGGTT TAATTAAAGT ATTAGTATGT ACGTCAACAT TAGCTTGGGG TGTAAATCTA 
CCTGCCCATG CTGTTATTAT AAGAGGAACA GAAATATATG ACGCAAAACA TGGTTCTTAT 
ATTGACTTAG ATATATTAGA TGTTTTACAA ATTTTTGGTC GTGCTGGTAG ACCACAATTT 
GATGTATCTG GTCATGCTGT TATTATTACT TCCCATAATA AATTATACCA TTACTTATCA 
TTATTAACAA ATCAAATACC TATAGAAAGC AATTTCATTA AATATTTAGC TGATAATTTA 
AATGCTGAAA TTGCATTGGG TACAATATCG AATGTAGAAG AAGCAATAAA ATGGTTAAGT 
TATACTTATT TATTCGTTCG AATGAAATTA AATTTTCATT TTTATGGAAT TCCTTATCGA 
GTTATTTTGG AAGATCAAAA TTTAGAACAA AAAAGAAGAG AACTAATTGA TCAAGCAGCT 
AAAGCATTAG ATGAAGCTAA AATGATTCGA TATAATATAA ATACTGGAGA TCTGAGCACA 
ACAAATTTAG GACGAATTGC GAGCCATTTT TATCTTAAAT ATGACACTAT TAAAATTTTT 
AATACACTTC TAAAACCAAT TATGACTGAA GCAGAAATAT TTTCTATGAT ATCATATTCT 
CAAGAATTTG AACAATTAAA AGTGCGAGAT GATGAAATGG AGGAATTACA ATATTTATTT 
GAAGAATATT GTAATATTAC AGTCCAAGGT GGTGTTGAAA ATATATATGG TAAAGTTAAT 
ATATTATTAC AAACGTATTT GTCACATGGT AGAGTTAATG CGTTTTCATT AATATCTGAT 
CAAGCATATA TTGTACAAAA TGCAGGACGG ATATTTCGAG CGTTGTTTGA AATTATGTTA 
GGACAAAATA ATGCAATAAT GGCTGGTCGT TTATTAGAAA TAGCAAAAGT ACTCGAAATA 
CAACAATGGA GTGATAGAAG TCCTCTATGT CAATTTTCTT GTTTATCTTT AGAAATTATT 
GACAAGATAG AACAATATGA TTTAACAGTT GAAAGATTAA ATAATATGAA TGTTAAAGAA 
ATTGGAGATA TTTTGCGTAA TCAGAAAGAA GCAATATTGG TGAAAAAATG TTGCAAAGAA 
TTACCTGCAT TAGAAATGGA ATCAAATTTA CAACCTATTA CTCGTACAGT TTTAAGAATA 
CATTTAAAAA TATATCCTCA ATTTCATTGG AATGATAATG TTCATGGTAA AACTTCAGAA 
CCATTCTGGA TATGGATAGA AGATCCAGAT AATAATTTTA TTTATCATCA TGAATATTTT 
ACTATGACAA GAAAAATGGT TTACAATAAT CTTGCACAAG AACTTGTGAT GACAATACCA 
TTGCATGAAC CATTGCCTAC TCAATACATA GTAAGAGCAA TAAGTGATCG CTGGTTGGGT 
TCTGAATATA TGTTACCTTT AACATTTCAT GATTTAATTT TACCTGAAGT ATATCCACCA 
TATACTGATT TGTTAGAAGT ACAACCTCTG CCAATAAAAG CATTGAAAGA ACCATTATTT 
GAAAAACTTT ATAAATTTTC TCATTTTAAT CCAATACAGA CACAAATTTT TCATTGTTTG 
TATCATACAG ACAATAACGT CCTATTAGGA GCTCCAACTG GTTCAGGAAA AACAATTGCT 
GCGGAAATTG CAATGTTTAG AGTATTTAAA CAATATCCAA CGCAAAAAAT TGTATATATT 
GCACCATTGA AAGCTTTAGT AAGAGAACGA ATGAAAGATT GGAAAATTAG ATTTGAAGAA 
CAATTAGGAA AAAAAGTAAT AGAATTAACT GGAGATGTAT CACCAGATAT TAAAGTTATA 
GCAAATGCAA ATATTATTAT AACTACTCCA GAAAAATGGG ATGGTATTAG TAGAAGTTGG 
CAAACAAGAC TTTATGTCAA AAATGTTGCG CTTATTATTA TAGATGAAAT ACATTTATTA 
GGGGAAGATC GTGGACCAGT ATTAGAAGTT ATTATTTCAA GAACTAATTT TATTTCTAGT 
CATACTTTAA AAAAATTAAG AGTTATTGGA CTTTCTACAG CATTAGCTAA TGCAGTAGAT 
TTAGCTAATT GGCTTGGTAT TAAAGAGATG GGTCTTTATA ATTTTCGTCC ATCTGTACGA 
CCTGTACCAT TAGATATTCA TATAACTGGA TTCCCAGGGA AAAATTATTG TCCTCGAATG 
GCAACAATGA ATAGACCAAC TTTTCAAGCA ATTAAACAAC ATGCTCCAAC TAGTCCGGCT 
TTAGTATTTG TTTCATCACG TAGACAAACA CGTTTAACTG CATTAGATTT GATTGCATAT 
CTTGCAGGAG AAGATAATCC TAAGCAATGG TTACATATGA GAGAAGAAGA AATGAATAAC 
ATTCTAAGTC ATATACGAGA TTCAAATTTG AAACTTACAC TTGCTTTTGG AATTGGACTT 
CATCATGCAG GTTTTCAGGA AAGAGATAGA AAAATTGTCG AAGAATTATT TGTTAACAAT 
AAAATTCAGG TATTGATTAC AACAGCTACT TTAGCTTGGG GAGTTAATTT CCCTGCACAC 
TTAGTTGTAA TAAAAGGAAC AGAATATTAT GATGGCAAAC AACACCGTTA TGTAGACATG 
CCAATCACAG ATGTACTACA AATGATGGGT CGTGCAGGAA GACCCCAGTT TGATAATTCA 
GGAGTGGCAG TAGTTTTAGT ACACGATTTA AAAAAAAATT ATTATAAGAA ATTTTTACAT 
CATCCATTTC CAGTAGAATC AAGTTTATTA GCTGTTCTGC CAGATCATAT AAATGCTGAA 
ATTGTAGCTA ATACAATTAA AAATAAACAA GAATTATTAG ATTATTTAAC ATGGACATAT 
TTTTTTAGAA GGTTAATGAA AAATCCTAGA TATTACAATT TAAGTATTTT AGAACCTTAT 
GCTATTAATC AATATCTCTC TTCTTTGGTT GATAATACAC TAAAAGTATT AATAGATTCA 
TATTGTGTGG CATTTGATGA GGAAGAACAA ATTTTATATC CACTTTCAAT GGGTAAAATA 
GCATCATTTT ATTACTTATC GCATCATACT ATGTTAATGT TTGTACAATC ATTACAAGAA 
TCTCTTACAT TGGAGCAATG TTTACATATT TTATGTAATT CTTATGAATA TAATGAATTA 
CCAGTAAGAC ACAATGAAGA ATTATTGAAT GAGGAATTAA GTAAAATGTG CCGTTATCAA 
ATAGATAATT ACTCATATAA TTCTCCTCAT ACCAAAGCAT TTTTATTACT TCAAGCACAT 
TTCTCAAGAC TTCCCTTACC ATGTGTAGAT TATTTTACTG ATTTAAAATC AGTTCTTGAT 
CAGGCAATTA GGATTATACA AGCAATGATA GATGCAGTTG CAGATCATGG ATGGTTAGCA 
AATACAATTA TGATAATGAA CATACTTCAA ATGATTATTC AAGCTCGATG GATTGATGAA 
TCAGCAATTA TTACATTGCC ATGTGTAAAT TCAGAACATT TAGAATTATT TTCAACATTA 
TCGCTTACTC TTCCTGAATT ATGTTTCAAT ATGCACAACA AAGATATTAA AATACTTAGA 
AAAGTACTTA ATAAAAGTTT TTCGCAAGAA CAAATATATC AGATATATCA AGTAATAAAA 
GAAATGCCAA TGCTCTGTAT TAAGTTAAGC TTAGAAAGTT ATGATGAAGA TAATGATAAT 
AATAAACAAA AAAATCAAAT ATTTATACCT CTAAAATCTG ATAATTTGGA TTATATAAAT 
ATTCACAAGG ATCAAGATTA CATATTAAAT ATTATAATGA AAAGAAAAAA TAAATCTAAT 
AATTTAAAAG CACACTGTCC TTTGTTTCAA AAAGGAAAGG ATGAAGGTTG GTTTTTAATT 
TTAGGTAATG TTTCTGATAA AGAATTATTA GTTCTAAAAC GAGCATCTGC AATAAATGAA 
CAACGGAAAT ATCAATTACA ATTCACAGCT CCATCAAAAT TAGGACAAAC AACTTTAACG 
TTTTATTTGA TTTCCGATTG TTACATAGGA CTGGATCAAC AGTATGATAT AAAAATAAAT 
GTAATATCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026299670.1
CDS	347..6478
Translation	MIYLEKKKSNVANLKVNSIGKIYVNMYLKMFYFILINYAKLLQKHLIITEIREKLNEETLILIKNGKENIKVLENGLFDPKFEYKSARVLWPDTNTLRSMSVGNINQSTDNFSMKYKNEATKLFKTEFDKTAYKENLKSYHTKNFADISLEKFEKIIITKLGSKNSENELVDLLGCESIEFIQYIVQHQKSIITLSSTPKIQKKCNIQGPVISGQVTVQSEKEKQLQKQIRKHEKKLNKTSKREGKGELEENGFEPLELTLKKNEALIEMHTPIFKKSINTDREKFPFVFDANMNSRNTTGYVSKQKIMLPQNVIRKDTETFEEIFIPIPESQPLDVDYKPIMVSSLDDIGQMAFNGIESLNRIQSIVFDVAYHTNENLLICAPTGAGKTNVAMLTIVHQLKQHIEYGQLMKNQFKIIYVTPMKALAAEMTANFSKKLHCLGISVRELTGDMQLTKSEIQQTQMIVTTPEKWDVVTRKGTGDISLTSIVKLLIIDEVHLLHGDRGPVVEALVARTLRQVESSQSMIRIVGLSATLPNYVDVARFLRVNSNIGLFYFDHRFRPVPLSQAFIGVKASSSLQEMNYMDNVCYNNVIDMVRQGYQVMVFVHARNATVRMANTLKDLAIKHGTLKLFIPEGQTKFINKAFAKSRTKYLSELFNNGLSVHHAGLLRSERNLIEKYFADGLIKVLVCTSTLAWGVNLPAHAVIIRGTEIYDAKHGSYIDLDILDVLQIFGRAGRPQFDVSGHAVIITSHNKLYHYLSLLTNQIPIESNFIKYLADNLNAEIALGTISNVEEAIKWLSYTYLFVRMKLNFHFYGIPYRVILEDQNLEQKRRELIDQAAKALDEAKMIRYNINTGDLSTTNLGRIASHFYLKYDTIKIFNTLLKPIMTEAEIFSMISYSQEFEQLKVRDDEMEELQYLFEEYCNITVQGGVENIYGKVNILLQTYLSHGRVNAFSLISDQAYIVQNAGRIFRALFEIMLGQNNAIMAGRLLEIAKVLEIQQWSDRSPLCQFSCLSLEIIDKIEQYDLTVERLNNMNVKEIGDILRNQKEAILVKKCCKELPALEMESNLQPITRTVLRIHLKIYPQFHWNDNVHGKTSEPFWIWIEDPDNNFIYHHEYFTMTRKMVYNNLAQELVMTIPLHEPLPTQYIVRAISDRWLGSEYMLPLTFHDLILPEVYPPYTDLLEVQPLPIKALKEPLFEKLYKFSHFNPIQTQIFHCLYHTDNNVLLGAPTGSGKTIAAEIAMFRVFKQYPTQKIVYIAPLKALVRERMKDWKIRFEEQLGKKVIELTGDVSPDIKVIANANIIITTPEKWDGISRSWQTRLYVKNVALIIIDEIHLLGEDRGPVLEVIISRTNFISSHTLKKLRVIGLSTALANAVDLANWLGIKEMGLYNFRPSVRPVPLDIHITGFPGKNYCPRMATMNRPTFQAIKQHAPTSPALVFVSSRRQTRLTALDLIAYLAGEDNPKQWLHMREEEMNNILSHIRDSNLKLTLAFGIGLHHAGFQERDRKIVEELFVNNKIQVLITTATLAWGVNFPAHLVVIKGTEYYDGKQHRYVDMPITDVLQMMGRAGRPQFDNSGVAVVLVHDLKKNYYKKFLHHPFPVESSLLAVLPDHINAEIVANTIKNKQELLDYLTWTYFFRRLMKNPRYYNLSILEPYAINQYLSSLVDNTLKVLIDSYCVAFDEEEQILYPLSMGKIASFYYLSHHTMLMFVQSLQESLTLEQCLHILCNSYEYNELPVRHNEELLNEELSKMCRYQIDNYSYNSPHTKAFLLLQAHFSRLPLPCVDYFTDLKSVLDQAIRIIQAMIDAVADHGWLANTIMIMNILQMIIQARWIDESAIITLPCVNSEHLELFSTLSLTLPELCFNMHNKDIKILRKVLNKSFSQEQIYQIYQVIKEMPMLCIKLSLESYDEDNDNNKQKNQIFIPLKSDNLDYINIHKDQDYILNIIMKRKNKSNNLKAHCPLFQKGKDEGWFLILGNVSDKELLVLKRASAINEQRKYQLQFTAPSKLGQTTLTFYLISDCYIGLDQQYDIKINVIS

Target ORF information:

RefSeq Version	XM_026443885.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026443885.1

ORF Insert Sequence:

ATGATTTATT TAGAAAAGAA AAAGAGCAAC GTAGCAAATC TAAAAGTGAA TTCAATTGGC 
AAGATTTATG TAAACATGTA TCTAAAAATG TTTTATTTCA TCTTAATAAA CTACGCCAAA 
TTGCTACAGA AGCACTTGAT AATTACAGAA ATTCGAGAAA AATTAAACGA AGAAACTTTA 
ATACTTATAA AAAACGGAAA AGAAAATATT AAAGTATTGG AAAATGGCTT ATTTGATCCA 
AAATTTGAAT ATAAATCTGC AAGAGTATTA TGGCCTGATA CTAATACACT TCGTAGTATG 
TCTGTGGGCA ACATTAATCA AAGTACAGAT AATTTCTCAA TGAAATATAA AAATGAAGCT 
ACAAAATTAT TTAAAACAGA ATTTGACAAG ACAGCATATA AGGAAAACTT GAAATCATAT 
CATACAAAGA ATTTTGCAGA TATATCTTTA GAAAAATTTG AGAAGATCAT AATTACTAAA 
TTAGGTTCTA AAAATTCAGA GAACGAATTA GTTGATTTAT TAGGTTGTGA ATCTATAGAA 
TTTATTCAAT ATATTGTTCA ACATCAGAAA AGTATTATAA CTTTAAGTTC TACTCCAAAA 
ATACAAAAAA AATGTAATAT TCAAGGACCT GTGATCAGTG GCCAAGTAAC AGTTCAATCT 
GAGAAAGAAA AACAATTACA AAAACAAATA CGGAAACACG AAAAAAAATT AAATAAAACG 
AGTAAACGAG AAGGAAAGGG AGAGTTGGAA GAAAATGGCT TTGAACCATT AGAATTGACT 
TTAAAAAAAA ATGAAGCATT AATAGAAATG CACACACCTA TATTTAAAAA AAGTATTAAT 
ACAGATCGAG AAAAATTTCC TTTTGTGTTT GATGCTAATA TGAACAGTAG AAATACAACT 
GGTTATGTAT CAAAACAAAA AATAATGTTA CCGCAAAATG TTATAAGGAA AGATACAGAA 
ACATTTGAAG AAATTTTTAT ACCTATTCCA GAATCTCAAC CACTTGATGT AGATTATAAA 
CCTATTATGG TATCTTCATT GGATGATATT GGACAAATGG CTTTTAATGG CATTGAATCA 
TTAAATCGAA TACAAAGTAT TGTGTTCGAT GTAGCATATC ATACTAATGA GAATTTATTA 
ATTTGTGCTC CAACAGGAGC TGGAAAGACT AATGTTGCTA TGTTAACTAT AGTACATCAA 
TTAAAGCAAC ATATTGAATA TGGTCAATTA ATGAAAAATC AGTTCAAAAT AATTTATGTA 
ACTCCAATGA AAGCTTTAGC AGCTGAAATG ACAGCAAATT TTAGCAAAAA ACTTCATTGT 
CTTGGTATAT CTGTTCGCGA GTTAACTGGA GATATGCAAT TAACCAAATC GGAAATTCAA 
CAAACACAGA TGATTGTTAC CACACCAGAA AAGTGGGATG TTGTTACTAG AAAAGGTACT 
GGAGATATTT CTCTCACAAG TATTGTAAAA CTGTTAATTA TTGATGAAGT ACATTTGTTA 
CATGGTGATA GAGGACCTGT GGTTGAAGCC TTAGTTGCTC GAACTTTGAG ACAAGTAGAA 
TCTTCTCAAA GTATGATCAG AATTGTTGGT CTTTCTGCAA CATTGCCAAA TTATGTAGAT 
GTTGCAAGAT TTTTAAGAGT GAATTCAAAC ATAGGACTTT TTTATTTTGA TCATCGATTT 
CGACCAGTAC CACTTAGTCA AGCGTTTATT GGTGTAAAAG CATCATCATC ATTACAAGAA 
ATGAATTACA TGGATAATGT ATGTTACAAT AATGTTATAG ATATGGTACG TCAAGGATAT 
CAAGTAATGG TATTTGTACA TGCACGAAAT GCTACTGTTA GAATGGCAAA TACACTAAAA 
GATTTAGCAA TAAAACATGG TACACTTAAA TTATTTATAC CTGAAGGTCA AACAAAATTT 
ATAAATAAAG CTTTTGCTAA ATCACGTACG AAGTATTTAA GTGAATTATT TAATAATGGA 
TTATCAGTAC ATCATGCTGG TTTATTACGT TCAGAGAGAA ATTTAATTGA AAAATATTTT 
GCAGATGGTT TAATTAAAGT ATTAGTATGT ACGTCAACAT TAGCTTGGGG TGTAAATCTA 
CCTGCCCATG CTGTTATTAT AAGAGGAACA GAAATATATG ACGCAAAACA TGGTTCTTAT 
ATTGACTTAG ATATATTAGA TGTTTTACAA ATTTTTGGTC GTGCTGGTAG ACCACAATTT 
GATGTATCTG GTCATGCTGT TATTATTACT TCCCATAATA AATTATACCA TTACTTATCA 
TTATTAACAA ATCAAATACC TATAGAAAGC AATTTCATTA AATATTTAGC TGATAATTTA 
AATGCTGAAA TTGCATTGGG TACAATATCG AATGTAGAAG AAGCAATAAA ATGGTTAAGT 
TATACTTATT TATTCGTTCG AATGAAATTA AATTTTCATT TTTATGGAAT TCCTTATCGA 
GTTATTTTGG AAGATCAAAA TTTAGAACAA AAAAGAAGAG AACTAATTGA TCAAGCAGCT 
AAAGCATTAG ATGAAGCTAA AATGATTCGA TATAATATAA ATACTGGAGA TCTGAGCACA 
ACAAATTTAG GACGAATTGC GAGCCATTTT TATCTTAAAT ATGACACTAT TAAAATTTTT 
AATACACTTC TAAAACCAAT TATGACTGAA GCAGAAATAT TTTCTATGAT ATCATATTCT 
CAAGAATTTG AACAATTAAA AGTGCGAGAT GATGAAATGG AGGAATTACA ATATTTATTT 
GAAGAATATT GTAATATTAC AGTCCAAGGT GGTGTTGAAA ATATATATGG TAAAGTTAAT 
ATATTATTAC AAACGTATTT GTCACATGGT AGAGTTAATG CGTTTTCATT AATATCTGAT 
CAAGCATATA TTGTACAAAA TGCAGGACGG ATATTTCGAG CGTTGTTTGA AATTATGTTA 
GGACAAAATA ATGCAATAAT GGCTGGTCGT TTATTAGAAA TAGCAAAAGT ACTCGAAATA 
CAACAATGGA GTGATAGAAG TCCTCTATGT CAATTTTCTT GTTTATCTTT AGAAATTATT 
GACAAGATAG AACAATATGA TTTAACAGTT GAAAGATTAA ATAATATGAA TGTTAAAGAA 
ATTGGAGATA TTTTGCGTAA TCAGAAAGAA GCAATATTGG TGAAAAAATG TTGCAAAGAA 
TTACCTGCAT TAGAAATGGA ATCAAATTTA CAACCTATTA CTCGTACAGT TTTAAGAATA 
CATTTAAAAA TATATCCTCA ATTTCATTGG AATGATAATG TTCATGGTAA AACTTCAGAA 
CCATTCTGGA TATGGATAGA AGATCCAGAT AATAATTTTA TTTATCATCA TGAATATTTT 
ACTATGACAA GAAAAATGGT TTACAATAAT CTTGCACAAG AACTTGTGAT GACAATACCA 
TTGCATGAAC CATTGCCTAC TCAATACATA GTAAGAGCAA TAAGTGATCG CTGGTTGGGT 
TCTGAATATA TGTTACCTTT AACATTTCAT GATTTAATTT TACCTGAAGT ATATCCACCA 
TATACTGATT TGTTAGAAGT ACAACCTCTG CCAATAAAAG CATTGAAAGA ACCATTATTT 
GAAAAACTTT ATAAATTTTC TCATTTTAAT CCAATACAGA CACAAATTTT TCATTGTTTG 
TATCATACAG ACAATAACGT CCTATTAGGA GCTCCAACTG GTTCAGGAAA AACAATTGCT 
GCGGAAATTG CAATGTTTAG AGTATTTAAA CAATATCCAA CGCAAAAAAT TGTATATATT 
GCACCATTGA AAGCTTTAGT AAGAGAACGA ATGAAAGATT GGAAAATTAG ATTTGAAGAA 
CAATTAGGAA AAAAAGTAAT AGAATTAACT GGAGATGTAT CACCAGATAT TAAAGTTATA 
GCAAATGCAA ATATTATTAT AACTACTCCA GAAAAATGGG ATGGTATTAG TAGAAGTTGG 
CAAACAAGAC TTTATGTCAA AAATGTTGCG CTTATTATTA TAGATGAAAT ACATTTATTA 
GGGGAAGATC GTGGACCAGT ATTAGAAGTT ATTATTTCAA GAACTAATTT TATTTCTAGT 
CATACTTTAA AAAAATTAAG AGTTATTGGA CTTTCTACAG CATTAGCTAA TGCAGTAGAT 
TTAGCTAATT GGCTTGGTAT TAAAGAGATG GGTCTTTATA ATTTTCGTCC ATCTGTACGA 
CCTGTACCAT TAGATATTCA TATAACTGGA TTCCCAGGGA AAAATTATTG TCCTCGAATG 
GCAACAATGA ATAGACCAAC TTTTCAAGCA ATTAAACAAC ATGCTCCAAC TAGTCCGGCT 
TTAGTATTTG TTTCATCACG TAGACAAACA CGTTTAACTG CATTAGATTT GATTGCATAT 
CTTGCAGGAG AAGATAATCC TAAGCAATGG TTACATATGA GAGAAGAAGA AATGAATAAC 
ATTCTAAGTC ATATACGAGA TTCAAATTTG AAACTTACAC TTGCTTTTGG AATTGGACTT 
CATCATGCAG GTTTTCAGGA AAGAGATAGA AAAATTGTCG AAGAATTATT TGTTAACAAT 
AAAATTCAGG TATTGATTAC AACAGCTACT TTAGCTTGGG GAGTTAATTT CCCTGCACAC 
TTAGTTGTAA TAAAAGGAAC AGAATATTAT GATGGCAAAC AACACCGTTA TGTAGACATG 
CCAATCACAG ATGTACTACA AATGATGGGT CGTGCAGGAA GACCCCAGTT TGATAATTCA 
GGAGTGGCAG TAGTTTTAGT ACACGATTTA AAAAAAAATT ATTATAAGAA ATTTTTACAT 
CATCCATTTC CAGTAGAATC AAGTTTATTA GCTGTTCTGC CAGATCATAT AAATGCTGAA 
ATTGTAGCTA ATACAATTAA AAATAAACAA GAATTATTAG ATTATTTAAC ATGGACATAT 
TTTTTTAGAA GGTTAATGAA AAATCCTAGA TATTACAATT TAAGTATTTT AGAACCTTAT 
GCTATTAATC AATATCTCTC TTCTTTGGTT GATAATACAC TAAAAGTATT AATAGATTCA 
TATTGTGTGG CATTTGATGA GGAAGAACAA ATTTTATATC CACTTTCAAT GGGTAAAATA 
GCATCATTTT ATTACTTATC GCATCATACT ATGTTAATGT TTGTACAATC ATTACAAGAA 
TCTCTTACAT TGGAGCAATG TTTACATATT TTATGTAATT CTTATGAATA TAATGAATTA 
CCAGTAAGAC ACAATGAAGA ATTATTGAAT GAGGAATTAA GTAAAATGTG CCGTTATCAA 
ATAGATAATT ACTCATATAA TTCTCCTCAT ACCAAAGCAT TTTTATTACT TCAAGCACAT 
TTCTCAAGAC TTCCCTTACC ATGTGTAGAT TATTTTACTG ATTTAAAATC AGTTCTTGAT 
CAGGCAATTA GGATTATACA AGCAATGATA GATGCAGTTG CAGATCATGG ATGGTTAGCA 
AATACAATTA TGATAATGAA CATACTTCAA ATGATTATTC AAGCTCGATG GATTGATGAA 
TCAGCAATTA TTACATTGCC ATGTGTAAAT TCAGAACATT TAGAATTATT TTCAACATTA 
TCGCTTACTC TTCCTGAATT ATGTTTCAAT ATGCACAACA AAGATATTAA AATACTTAGA 
AAAGTACTTA ATAAAAGTTT TTCGCAAGAA CAAATATATC AGATATATCA AGTAATAAAA 
GAAATGCCAA TGCTCTGTAT TAAGTTAAGC TTAGAAAGTT ATGATGAAGA TAATGATAAT 
AATAAACAAA AAAATCAAAT ATTTATACCT CTAAAATCTG ATAATTTGGA TTATATAAAT 
ATTCACAAGG ATCAAGATTA CATATTAAAT ATTATAATGA AAAGAAAAAA TAAATCTAAT 
AATTTAAAAG CACACTGTCC TTTGTTTCAA AAAGGAAAGG ATGAAGGTTG GTTTTTAATT 
TTAGGTAATG TTTCTGATAA AGAATTATTA GTTCTAAAAC GAGCATCTGC AATAAATGAA 
CAACGGAAAT ATCAATTACA ATTCACAGCT CCATCAAAAT TAGGACAAAC AACTTTAACG 
TTTTATTTGA TTTCCGATTG TTACATAGGA CTGGATCAAC AGTATGATAT AAAAATAAAT 
GTAATATCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC552814 ( XM_026443883.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026299668.1 Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X4, mRNA.

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CloneID	OHd25553
Clone ID Related Accession (Same CDS sequence)	XM_026443884.1 , XM_026443885.1 , XM_026443883.1
Accession Version	XM_026443883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	activating signal cointegrator 1 complex subunit 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037648.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTATT TAGAAAAGAA AAAGAGCAAC GTAGCAAATC TAAAAGTGAA TTCAATTGGC 
AAGATTTATG TAAACATGTA TCTAAAAATG TTTTATTTCA TCTTAATAAA CTACGCCAAA 
TTGCTACAGA AGCACTTGAT AATTACAGAA ATTCGAGAAA AATTAAACGA AGAAACTTTA 
ATACTTATAA AAAACGGAAA AGAAAATATT AAAGTATTGG AAAATGGCTT ATTTGATCCA 
AAATTTGAAT ATAAATCTGC AAGAGTATTA TGGCCTGATA CTAATACACT TCGTAGTATG 
TCTGTGGGCA ACATTAATCA AAGTACAGAT AATTTCTCAA TGAAATATAA AAATGAAGCT 
ACAAAATTAT TTAAAACAGA ATTTGACAAG ACAGCATATA AGGAAAACTT GAAATCATAT 
CATACAAAGA ATTTTGCAGA TATATCTTTA GAAAAATTTG AGAAGATCAT AATTACTAAA 
TTAGGTTCTA AAAATTCAGA GAACGAATTA GTTGATTTAT TAGGTTGTGA ATCTATAGAA 
TTTATTCAAT ATATTGTTCA ACATCAGAAA AGTATTATAA CTTTAAGTTC TACTCCAAAA 
ATACAAAAAA AATGTAATAT TCAAGGACCT GTGATCAGTG GCCAAGTAAC AGTTCAATCT 
GAGAAAGAAA AACAATTACA AAAACAAATA CGGAAACACG AAAAAAAATT AAATAAAACG 
AGTAAACGAG AAGGAAAGGG AGAGTTGGAA GAAAATGGCT TTGAACCATT AGAATTGACT 
TTAAAAAAAA ATGAAGCATT AATAGAAATG CACACACCTA TATTTAAAAA AAGTATTAAT 
ACAGATCGAG AAAAATTTCC TTTTGTGTTT GATGCTAATA TGAACAGTAG AAATACAACT 
GGTTATGTAT CAAAACAAAA AATAATGTTA CCGCAAAATG TTATAAGGAA AGATACAGAA 
ACATTTGAAG AAATTTTTAT ACCTATTCCA GAATCTCAAC CACTTGATGT AGATTATAAA 
CCTATTATGG TATCTTCATT GGATGATATT GGACAAATGG CTTTTAATGG CATTGAATCA 
TTAAATCGAA TACAAAGTAT TGTGTTCGAT GTAGCATATC ATACTAATGA GAATTTATTA 
ATTTGTGCTC CAACAGGAGC TGGAAAGACT AATGTTGCTA TGTTAACTAT AGTACATCAA 
TTAAAGCAAC ATATTGAATA TGGTCAATTA ATGAAAAATC AGTTCAAAAT AATTTATGTA 
ACTCCAATGA AAGCTTTAGC AGCTGAAATG ACAGCAAATT TTAGCAAAAA ACTTCATTGT 
CTTGGTATAT CTGTTCGCGA GTTAACTGGA GATATGCAAT TAACCAAATC GGAAATTCAA 
CAAACACAGA TGATTGTTAC CACACCAGAA AAGTGGGATG TTGTTACTAG AAAAGGTACT 
GGAGATATTT CTCTCACAAG TATTGTAAAA CTGTTAATTA TTGATGAAGT ACATTTGTTA 
CATGGTGATA GAGGACCTGT GGTTGAAGCC TTAGTTGCTC GAACTTTGAG ACAAGTAGAA 
TCTTCTCAAA GTATGATCAG AATTGTTGGT CTTTCTGCAA CATTGCCAAA TTATGTAGAT 
GTTGCAAGAT TTTTAAGAGT GAATTCAAAC ATAGGACTTT TTTATTTTGA TCATCGATTT 
CGACCAGTAC CACTTAGTCA AGCGTTTATT GGTGTAAAAG CATCATCATC ATTACAAGAA 
ATGAATTACA TGGATAATGT ATGTTACAAT AATGTTATAG ATATGGTACG TCAAGGATAT 
CAAGTAATGG TATTTGTACA TGCACGAAAT GCTACTGTTA GAATGGCAAA TACACTAAAA 
GATTTAGCAA TAAAACATGG TACACTTAAA TTATTTATAC CTGAAGGTCA AACAAAATTT 
ATAAATAAAG CTTTTGCTAA ATCACGTACG AAGTATTTAA GTGAATTATT TAATAATGGA 
TTATCAGTAC ATCATGCTGG TTTATTACGT TCAGAGAGAA ATTTAATTGA AAAATATTTT 
GCAGATGGTT TAATTAAAGT ATTAGTATGT ACGTCAACAT TAGCTTGGGG TGTAAATCTA 
CCTGCCCATG CTGTTATTAT AAGAGGAACA GAAATATATG ACGCAAAACA TGGTTCTTAT 
ATTGACTTAG ATATATTAGA TGTTTTACAA ATTTTTGGTC GTGCTGGTAG ACCACAATTT 
GATGTATCTG GTCATGCTGT TATTATTACT TCCCATAATA AATTATACCA TTACTTATCA 
TTATTAACAA ATCAAATACC TATAGAAAGC AATTTCATTA AATATTTAGC TGATAATTTA 
AATGCTGAAA TTGCATTGGG TACAATATCG AATGTAGAAG AAGCAATAAA ATGGTTAAGT 
TATACTTATT TATTCGTTCG AATGAAATTA AATTTTCATT TTTATGGAAT TCCTTATCGA 
GTTATTTTGG AAGATCAAAA TTTAGAACAA AAAAGAAGAG AACTAATTGA TCAAGCAGCT 
AAAGCATTAG ATGAAGCTAA AATGATTCGA TATAATATAA ATACTGGAGA TCTGAGCACA 
ACAAATTTAG GACGAATTGC GAGCCATTTT TATCTTAAAT ATGACACTAT TAAAATTTTT 
AATACACTTC TAAAACCAAT TATGACTGAA GCAGAAATAT TTTCTATGAT ATCATATTCT 
CAAGAATTTG AACAATTAAA AGTGCGAGAT GATGAAATGG AGGAATTACA ATATTTATTT 
GAAGAATATT GTAATATTAC AGTCCAAGGT GGTGTTGAAA ATATATATGG TAAAGTTAAT 
ATATTATTAC AAACGTATTT GTCACATGGT AGAGTTAATG CGTTTTCATT AATATCTGAT 
CAAGCATATA TTGTACAAAA TGCAGGACGG ATATTTCGAG CGTTGTTTGA AATTATGTTA 
GGACAAAATA ATGCAATAAT GGCTGGTCGT TTATTAGAAA TAGCAAAAGT ACTCGAAATA 
CAACAATGGA GTGATAGAAG TCCTCTATGT CAATTTTCTT GTTTATCTTT AGAAATTATT 
GACAAGATAG AACAATATGA TTTAACAGTT GAAAGATTAA ATAATATGAA TGTTAAAGAA 
ATTGGAGATA TTTTGCGTAA TCAGAAAGAA GCAATATTGG TGAAAAAATG TTGCAAAGAA 
TTACCTGCAT TAGAAATGGA ATCAAATTTA CAACCTATTA CTCGTACAGT TTTAAGAATA 
CATTTAAAAA TATATCCTCA ATTTCATTGG AATGATAATG TTCATGGTAA AACTTCAGAA 
CCATTCTGGA TATGGATAGA AGATCCAGAT AATAATTTTA TTTATCATCA TGAATATTTT 
ACTATGACAA GAAAAATGGT TTACAATAAT CTTGCACAAG AACTTGTGAT GACAATACCA 
TTGCATGAAC CATTGCCTAC TCAATACATA GTAAGAGCAA TAAGTGATCG CTGGTTGGGT 
TCTGAATATA TGTTACCTTT AACATTTCAT GATTTAATTT TACCTGAAGT ATATCCACCA 
TATACTGATT TGTTAGAAGT ACAACCTCTG CCAATAAAAG CATTGAAAGA ACCATTATTT 
GAAAAACTTT ATAAATTTTC TCATTTTAAT CCAATACAGA CACAAATTTT TCATTGTTTG 
TATCATACAG ACAATAACGT CCTATTAGGA GCTCCAACTG GTTCAGGAAA AACAATTGCT 
GCGGAAATTG CAATGTTTAG AGTATTTAAA CAATATCCAA CGCAAAAAAT TGTATATATT 
GCACCATTGA AAGCTTTAGT AAGAGAACGA ATGAAAGATT GGAAAATTAG ATTTGAAGAA 
CAATTAGGAA AAAAAGTAAT AGAATTAACT GGAGATGTAT CACCAGATAT TAAAGTTATA 
GCAAATGCAA ATATTATTAT AACTACTCCA GAAAAATGGG ATGGTATTAG TAGAAGTTGG 
CAAACAAGAC TTTATGTCAA AAATGTTGCG CTTATTATTA TAGATGAAAT ACATTTATTA 
GGGGAAGATC GTGGACCAGT ATTAGAAGTT ATTATTTCAA GAACTAATTT TATTTCTAGT 
CATACTTTAA AAAAATTAAG AGTTATTGGA CTTTCTACAG CATTAGCTAA TGCAGTAGAT 
TTAGCTAATT GGCTTGGTAT TAAAGAGATG GGTCTTTATA ATTTTCGTCC ATCTGTACGA 
CCTGTACCAT TAGATATTCA TATAACTGGA TTCCCAGGGA AAAATTATTG TCCTCGAATG 
GCAACAATGA ATAGACCAAC TTTTCAAGCA ATTAAACAAC ATGCTCCAAC TAGTCCGGCT 
TTAGTATTTG TTTCATCACG TAGACAAACA CGTTTAACTG CATTAGATTT GATTGCATAT 
CTTGCAGGAG AAGATAATCC TAAGCAATGG TTACATATGA GAGAAGAAGA AATGAATAAC 
ATTCTAAGTC ATATACGAGA TTCAAATTTG AAACTTACAC TTGCTTTTGG AATTGGACTT 
CATCATGCAG GTTTTCAGGA AAGAGATAGA AAAATTGTCG AAGAATTATT TGTTAACAAT 
AAAATTCAGG TATTGATTAC AACAGCTACT TTAGCTTGGG GAGTTAATTT CCCTGCACAC 
TTAGTTGTAA TAAAAGGAAC AGAATATTAT GATGGCAAAC AACACCGTTA TGTAGACATG 
CCAATCACAG ATGTACTACA AATGATGGGT CGTGCAGGAA GACCCCAGTT TGATAATTCA 
GGAGTGGCAG TAGTTTTAGT ACACGATTTA AAAAAAAATT ATTATAAGAA ATTTTTACAT 
CATCCATTTC CAGTAGAATC AAGTTTATTA GCTGTTCTGC CAGATCATAT AAATGCTGAA 
ATTGTAGCTA ATACAATTAA AAATAAACAA GAATTATTAG ATTATTTAAC ATGGACATAT 
TTTTTTAGAA GGTTAATGAA AAATCCTAGA TATTACAATT TAAGTATTTT AGAACCTTAT 
GCTATTAATC AATATCTCTC TTCTTTGGTT GATAATACAC TAAAAGTATT AATAGATTCA 
TATTGTGTGG CATTTGATGA GGAAGAACAA ATTTTATATC CACTTTCAAT GGGTAAAATA 
GCATCATTTT ATTACTTATC GCATCATACT ATGTTAATGT TTGTACAATC ATTACAAGAA 
TCTCTTACAT TGGAGCAATG TTTACATATT TTATGTAATT CTTATGAATA TAATGAATTA 
CCAGTAAGAC ACAATGAAGA ATTATTGAAT GAGGAATTAA GTAAAATGTG CCGTTATCAA 
ATAGATAATT ACTCATATAA TTCTCCTCAT ACCAAAGCAT TTTTATTACT TCAAGCACAT 
TTCTCAAGAC TTCCCTTACC ATGTGTAGAT TATTTTACTG ATTTAAAATC AGTTCTTGAT 
CAGGCAATTA GGATTATACA AGCAATGATA GATGCAGTTG CAGATCATGG ATGGTTAGCA 
AATACAATTA TGATAATGAA CATACTTCAA ATGATTATTC AAGCTCGATG GATTGATGAA 
TCAGCAATTA TTACATTGCC ATGTGTAAAT TCAGAACATT TAGAATTATT TTCAACATTA 
TCGCTTACTC TTCCTGAATT ATGTTTCAAT ATGCACAACA AAGATATTAA AATACTTAGA 
AAAGTACTTA ATAAAAGTTT TTCGCAAGAA CAAATATATC AGATATATCA AGTAATAAAA 
GAAATGCCAA TGCTCTGTAT TAAGTTAAGC TTAGAAAGTT ATGATGAAGA TAATGATAAT 
AATAAACAAA AAAATCAAAT ATTTATACCT CTAAAATCTG ATAATTTGGA TTATATAAAT 
ATTCACAAGG ATCAAGATTA CATATTAAAT ATTATAATGA AAAGAAAAAA TAAATCTAAT 
AATTTAAAAG CACACTGTCC TTTGTTTCAA AAAGGAAAGG ATGAAGGTTG GTTTTTAATT 
TTAGGTAATG TTTCTGATAA AGAATTATTA GTTCTAAAAC GAGCATCTGC AATAAATGAA 
CAACGGAAAT ATCAATTACA ATTCACAGCT CCATCAAAAT TAGGACAAAC AACTTTAACG 
TTTTATTTGA TTTCCGATTG TTACATAGGA CTGGATCAAC AGTATGATAT AAAAATAAAT 
GTAATATCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026299668.1
CDS	356..6487
Translation	MIYLEKKKSNVANLKVNSIGKIYVNMYLKMFYFILINYAKLLQKHLIITEIREKLNEETLILIKNGKENIKVLENGLFDPKFEYKSARVLWPDTNTLRSMSVGNINQSTDNFSMKYKNEATKLFKTEFDKTAYKENLKSYHTKNFADISLEKFEKIIITKLGSKNSENELVDLLGCESIEFIQYIVQHQKSIITLSSTPKIQKKCNIQGPVISGQVTVQSEKEKQLQKQIRKHEKKLNKTSKREGKGELEENGFEPLELTLKKNEALIEMHTPIFKKSINTDREKFPFVFDANMNSRNTTGYVSKQKIMLPQNVIRKDTETFEEIFIPIPESQPLDVDYKPIMVSSLDDIGQMAFNGIESLNRIQSIVFDVAYHTNENLLICAPTGAGKTNVAMLTIVHQLKQHIEYGQLMKNQFKIIYVTPMKALAAEMTANFSKKLHCLGISVRELTGDMQLTKSEIQQTQMIVTTPEKWDVVTRKGTGDISLTSIVKLLIIDEVHLLHGDRGPVVEALVARTLRQVESSQSMIRIVGLSATLPNYVDVARFLRVNSNIGLFYFDHRFRPVPLSQAFIGVKASSSLQEMNYMDNVCYNNVIDMVRQGYQVMVFVHARNATVRMANTLKDLAIKHGTLKLFIPEGQTKFINKAFAKSRTKYLSELFNNGLSVHHAGLLRSERNLIEKYFADGLIKVLVCTSTLAWGVNLPAHAVIIRGTEIYDAKHGSYIDLDILDVLQIFGRAGRPQFDVSGHAVIITSHNKLYHYLSLLTNQIPIESNFIKYLADNLNAEIALGTISNVEEAIKWLSYTYLFVRMKLNFHFYGIPYRVILEDQNLEQKRRELIDQAAKALDEAKMIRYNINTGDLSTTNLGRIASHFYLKYDTIKIFNTLLKPIMTEAEIFSMISYSQEFEQLKVRDDEMEELQYLFEEYCNITVQGGVENIYGKVNILLQTYLSHGRVNAFSLISDQAYIVQNAGRIFRALFEIMLGQNNAIMAGRLLEIAKVLEIQQWSDRSPLCQFSCLSLEIIDKIEQYDLTVERLNNMNVKEIGDILRNQKEAILVKKCCKELPALEMESNLQPITRTVLRIHLKIYPQFHWNDNVHGKTSEPFWIWIEDPDNNFIYHHEYFTMTRKMVYNNLAQELVMTIPLHEPLPTQYIVRAISDRWLGSEYMLPLTFHDLILPEVYPPYTDLLEVQPLPIKALKEPLFEKLYKFSHFNPIQTQIFHCLYHTDNNVLLGAPTGSGKTIAAEIAMFRVFKQYPTQKIVYIAPLKALVRERMKDWKIRFEEQLGKKVIELTGDVSPDIKVIANANIIITTPEKWDGISRSWQTRLYVKNVALIIIDEIHLLGEDRGPVLEVIISRTNFISSHTLKKLRVIGLSTALANAVDLANWLGIKEMGLYNFRPSVRPVPLDIHITGFPGKNYCPRMATMNRPTFQAIKQHAPTSPALVFVSSRRQTRLTALDLIAYLAGEDNPKQWLHMREEEMNNILSHIRDSNLKLTLAFGIGLHHAGFQERDRKIVEELFVNNKIQVLITTATLAWGVNFPAHLVVIKGTEYYDGKQHRYVDMPITDVLQMMGRAGRPQFDNSGVAVVLVHDLKKNYYKKFLHHPFPVESSLLAVLPDHINAEIVANTIKNKQELLDYLTWTYFFRRLMKNPRYYNLSILEPYAINQYLSSLVDNTLKVLIDSYCVAFDEEEQILYPLSMGKIASFYYLSHHTMLMFVQSLQESLTLEQCLHILCNSYEYNELPVRHNEELLNEELSKMCRYQIDNYSYNSPHTKAFLLLQAHFSRLPLPCVDYFTDLKSVLDQAIRIIQAMIDAVADHGWLANTIMIMNILQMIIQARWIDESAIITLPCVNSEHLELFSTLSLTLPELCFNMHNKDIKILRKVLNKSFSQEQIYQIYQVIKEMPMLCIKLSLESYDEDNDNNKQKNQIFIPLKSDNLDYINIHKDQDYILNIIMKRKNKSNNLKAHCPLFQKGKDEGWFLILGNVSDKELLVLKRASAINEQRKYQLQFTAPSKLGQTTLTFYLISDCYIGLDQQYDIKINVIS

Target ORF information:

RefSeq Version	XM_026443883.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera activating signal cointegrator 1 complex subunit 3 (LOC552814), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026443883.1

ORF Insert Sequence:

ATGATTTATT TAGAAAAGAA AAAGAGCAAC GTAGCAAATC TAAAAGTGAA TTCAATTGGC 
AAGATTTATG TAAACATGTA TCTAAAAATG TTTTATTTCA TCTTAATAAA CTACGCCAAA 
TTGCTACAGA AGCACTTGAT AATTACAGAA ATTCGAGAAA AATTAAACGA AGAAACTTTA 
ATACTTATAA AAAACGGAAA AGAAAATATT AAAGTATTGG AAAATGGCTT ATTTGATCCA 
AAATTTGAAT ATAAATCTGC AAGAGTATTA TGGCCTGATA CTAATACACT TCGTAGTATG 
TCTGTGGGCA ACATTAATCA AAGTACAGAT AATTTCTCAA TGAAATATAA AAATGAAGCT 
ACAAAATTAT TTAAAACAGA ATTTGACAAG ACAGCATATA AGGAAAACTT GAAATCATAT 
CATACAAAGA ATTTTGCAGA TATATCTTTA GAAAAATTTG AGAAGATCAT AATTACTAAA 
TTAGGTTCTA AAAATTCAGA GAACGAATTA GTTGATTTAT TAGGTTGTGA ATCTATAGAA 
TTTATTCAAT ATATTGTTCA ACATCAGAAA AGTATTATAA CTTTAAGTTC TACTCCAAAA 
ATACAAAAAA AATGTAATAT TCAAGGACCT GTGATCAGTG GCCAAGTAAC AGTTCAATCT 
GAGAAAGAAA AACAATTACA AAAACAAATA CGGAAACACG AAAAAAAATT AAATAAAACG 
AGTAAACGAG AAGGAAAGGG AGAGTTGGAA GAAAATGGCT TTGAACCATT AGAATTGACT 
TTAAAAAAAA ATGAAGCATT AATAGAAATG CACACACCTA TATTTAAAAA AAGTATTAAT 
ACAGATCGAG AAAAATTTCC TTTTGTGTTT GATGCTAATA TGAACAGTAG AAATACAACT 
GGTTATGTAT CAAAACAAAA AATAATGTTA CCGCAAAATG TTATAAGGAA AGATACAGAA 
ACATTTGAAG AAATTTTTAT ACCTATTCCA GAATCTCAAC CACTTGATGT AGATTATAAA 
CCTATTATGG TATCTTCATT GGATGATATT GGACAAATGG CTTTTAATGG CATTGAATCA 
TTAAATCGAA TACAAAGTAT TGTGTTCGAT GTAGCATATC ATACTAATGA GAATTTATTA 
ATTTGTGCTC CAACAGGAGC TGGAAAGACT AATGTTGCTA TGTTAACTAT AGTACATCAA 
TTAAAGCAAC ATATTGAATA TGGTCAATTA ATGAAAAATC AGTTCAAAAT AATTTATGTA 
ACTCCAATGA AAGCTTTAGC AGCTGAAATG ACAGCAAATT TTAGCAAAAA ACTTCATTGT 
CTTGGTATAT CTGTTCGCGA GTTAACTGGA GATATGCAAT TAACCAAATC GGAAATTCAA 
CAAACACAGA TGATTGTTAC CACACCAGAA AAGTGGGATG TTGTTACTAG AAAAGGTACT 
GGAGATATTT CTCTCACAAG TATTGTAAAA CTGTTAATTA TTGATGAAGT ACATTTGTTA 
CATGGTGATA GAGGACCTGT GGTTGAAGCC TTAGTTGCTC GAACTTTGAG ACAAGTAGAA 
TCTTCTCAAA GTATGATCAG AATTGTTGGT CTTTCTGCAA CATTGCCAAA TTATGTAGAT 
GTTGCAAGAT TTTTAAGAGT GAATTCAAAC ATAGGACTTT TTTATTTTGA TCATCGATTT 
CGACCAGTAC CACTTAGTCA AGCGTTTATT GGTGTAAAAG CATCATCATC ATTACAAGAA 
ATGAATTACA TGGATAATGT ATGTTACAAT AATGTTATAG ATATGGTACG TCAAGGATAT 
CAAGTAATGG TATTTGTACA TGCACGAAAT GCTACTGTTA GAATGGCAAA TACACTAAAA 
GATTTAGCAA TAAAACATGG TACACTTAAA TTATTTATAC CTGAAGGTCA AACAAAATTT 
ATAAATAAAG CTTTTGCTAA ATCACGTACG AAGTATTTAA GTGAATTATT TAATAATGGA 
TTATCAGTAC ATCATGCTGG TTTATTACGT TCAGAGAGAA ATTTAATTGA AAAATATTTT 
GCAGATGGTT TAATTAAAGT ATTAGTATGT ACGTCAACAT TAGCTTGGGG TGTAAATCTA 
CCTGCCCATG CTGTTATTAT AAGAGGAACA GAAATATATG ACGCAAAACA TGGTTCTTAT 
ATTGACTTAG ATATATTAGA TGTTTTACAA ATTTTTGGTC GTGCTGGTAG ACCACAATTT 
GATGTATCTG GTCATGCTGT TATTATTACT TCCCATAATA AATTATACCA TTACTTATCA 
TTATTAACAA ATCAAATACC TATAGAAAGC AATTTCATTA AATATTTAGC TGATAATTTA 
AATGCTGAAA TTGCATTGGG TACAATATCG AATGTAGAAG AAGCAATAAA ATGGTTAAGT 
TATACTTATT TATTCGTTCG AATGAAATTA AATTTTCATT TTTATGGAAT TCCTTATCGA 
GTTATTTTGG AAGATCAAAA TTTAGAACAA AAAAGAAGAG AACTAATTGA TCAAGCAGCT 
AAAGCATTAG ATGAAGCTAA AATGATTCGA TATAATATAA ATACTGGAGA TCTGAGCACA 
ACAAATTTAG GACGAATTGC GAGCCATTTT TATCTTAAAT ATGACACTAT TAAAATTTTT 
AATACACTTC TAAAACCAAT TATGACTGAA GCAGAAATAT TTTCTATGAT ATCATATTCT 
CAAGAATTTG AACAATTAAA AGTGCGAGAT GATGAAATGG AGGAATTACA ATATTTATTT 
GAAGAATATT GTAATATTAC AGTCCAAGGT GGTGTTGAAA ATATATATGG TAAAGTTAAT 
ATATTATTAC AAACGTATTT GTCACATGGT AGAGTTAATG CGTTTTCATT AATATCTGAT 
CAAGCATATA TTGTACAAAA TGCAGGACGG ATATTTCGAG CGTTGTTTGA AATTATGTTA 
GGACAAAATA ATGCAATAAT GGCTGGTCGT TTATTAGAAA TAGCAAAAGT ACTCGAAATA 
CAACAATGGA GTGATAGAAG TCCTCTATGT CAATTTTCTT GTTTATCTTT AGAAATTATT 
GACAAGATAG AACAATATGA TTTAACAGTT GAAAGATTAA ATAATATGAA TGTTAAAGAA 
ATTGGAGATA TTTTGCGTAA TCAGAAAGAA GCAATATTGG TGAAAAAATG TTGCAAAGAA 
TTACCTGCAT TAGAAATGGA ATCAAATTTA CAACCTATTA CTCGTACAGT TTTAAGAATA 
CATTTAAAAA TATATCCTCA ATTTCATTGG AATGATAATG TTCATGGTAA AACTTCAGAA 
CCATTCTGGA TATGGATAGA AGATCCAGAT AATAATTTTA TTTATCATCA TGAATATTTT 
ACTATGACAA GAAAAATGGT TTACAATAAT CTTGCACAAG AACTTGTGAT GACAATACCA 
TTGCATGAAC CATTGCCTAC TCAATACATA GTAAGAGCAA TAAGTGATCG CTGGTTGGGT 
TCTGAATATA TGTTACCTTT AACATTTCAT GATTTAATTT TACCTGAAGT ATATCCACCA 
TATACTGATT TGTTAGAAGT ACAACCTCTG CCAATAAAAG CATTGAAAGA ACCATTATTT 
GAAAAACTTT ATAAATTTTC TCATTTTAAT CCAATACAGA CACAAATTTT TCATTGTTTG 
TATCATACAG ACAATAACGT CCTATTAGGA GCTCCAACTG GTTCAGGAAA AACAATTGCT 
GCGGAAATTG CAATGTTTAG AGTATTTAAA CAATATCCAA CGCAAAAAAT TGTATATATT 
GCACCATTGA AAGCTTTAGT AAGAGAACGA ATGAAAGATT GGAAAATTAG ATTTGAAGAA 
CAATTAGGAA AAAAAGTAAT AGAATTAACT GGAGATGTAT CACCAGATAT TAAAGTTATA 
GCAAATGCAA ATATTATTAT AACTACTCCA GAAAAATGGG ATGGTATTAG TAGAAGTTGG 
CAAACAAGAC TTTATGTCAA AAATGTTGCG CTTATTATTA TAGATGAAAT ACATTTATTA 
GGGGAAGATC GTGGACCAGT ATTAGAAGTT ATTATTTCAA GAACTAATTT TATTTCTAGT 
CATACTTTAA AAAAATTAAG AGTTATTGGA CTTTCTACAG CATTAGCTAA TGCAGTAGAT 
TTAGCTAATT GGCTTGGTAT TAAAGAGATG GGTCTTTATA ATTTTCGTCC ATCTGTACGA 
CCTGTACCAT TAGATATTCA TATAACTGGA TTCCCAGGGA AAAATTATTG TCCTCGAATG 
GCAACAATGA ATAGACCAAC TTTTCAAGCA ATTAAACAAC ATGCTCCAAC TAGTCCGGCT 
TTAGTATTTG TTTCATCACG TAGACAAACA CGTTTAACTG CATTAGATTT GATTGCATAT 
CTTGCAGGAG AAGATAATCC TAAGCAATGG TTACATATGA GAGAAGAAGA AATGAATAAC 
ATTCTAAGTC ATATACGAGA TTCAAATTTG AAACTTACAC TTGCTTTTGG AATTGGACTT 
CATCATGCAG GTTTTCAGGA AAGAGATAGA AAAATTGTCG AAGAATTATT TGTTAACAAT 
AAAATTCAGG TATTGATTAC AACAGCTACT TTAGCTTGGG GAGTTAATTT CCCTGCACAC 
TTAGTTGTAA TAAAAGGAAC AGAATATTAT GATGGCAAAC AACACCGTTA TGTAGACATG 
CCAATCACAG ATGTACTACA AATGATGGGT CGTGCAGGAA GACCCCAGTT TGATAATTCA 
GGAGTGGCAG TAGTTTTAGT ACACGATTTA AAAAAAAATT ATTATAAGAA ATTTTTACAT 
CATCCATTTC CAGTAGAATC AAGTTTATTA GCTGTTCTGC CAGATCATAT AAATGCTGAA 
ATTGTAGCTA ATACAATTAA AAATAAACAA GAATTATTAG ATTATTTAAC ATGGACATAT 
TTTTTTAGAA GGTTAATGAA AAATCCTAGA TATTACAATT TAAGTATTTT AGAACCTTAT 
GCTATTAATC AATATCTCTC TTCTTTGGTT GATAATACAC TAAAAGTATT AATAGATTCA 
TATTGTGTGG CATTTGATGA GGAAGAACAA ATTTTATATC CACTTTCAAT GGGTAAAATA 
GCATCATTTT ATTACTTATC GCATCATACT ATGTTAATGT TTGTACAATC ATTACAAGAA 
TCTCTTACAT TGGAGCAATG TTTACATATT TTATGTAATT CTTATGAATA TAATGAATTA 
CCAGTAAGAC ACAATGAAGA ATTATTGAAT GAGGAATTAA GTAAAATGTG CCGTTATCAA 
ATAGATAATT ACTCATATAA TTCTCCTCAT ACCAAAGCAT TTTTATTACT TCAAGCACAT 
TTCTCAAGAC TTCCCTTACC ATGTGTAGAT TATTTTACTG ATTTAAAATC AGTTCTTGAT 
CAGGCAATTA GGATTATACA AGCAATGATA GATGCAGTTG CAGATCATGG ATGGTTAGCA 
AATACAATTA TGATAATGAA CATACTTCAA ATGATTATTC AAGCTCGATG GATTGATGAA 
TCAGCAATTA TTACATTGCC ATGTGTAAAT TCAGAACATT TAGAATTATT TTCAACATTA 
TCGCTTACTC TTCCTGAATT ATGTTTCAAT ATGCACAACA AAGATATTAA AATACTTAGA 
AAAGTACTTA ATAAAAGTTT TTCGCAAGAA CAAATATATC AGATATATCA AGTAATAAAA 
GAAATGCCAA TGCTCTGTAT TAAGTTAAGC TTAGAAAGTT ATGATGAAGA TAATGATAAT 
AATAAACAAA AAAATCAAAT ATTTATACCT CTAAAATCTG ATAATTTGGA TTATATAAAT 
ATTCACAAGG ATCAAGATTA CATATTAAAT ATTATAATGA AAAGAAAAAA TAAATCTAAT 
AATTTAAAAG CACACTGTCC TTTGTTTCAA AAAGGAAAGG ATGAAGGTTG GTTTTTAATT 
TTAGGTAATG TTTCTGATAA AGAATTATTA GTTCTAAAAC GAGCATCTGC AATAAATGAA 
CAACGGAAAT ATCAATTACA ATTCACAGCT CCATCAAAAT TAGGACAAAC AACTTTAACG 
TTTTATTTGA TTTCCGATTG TTACATAGGA CTGGATCAAC AGTATGATAT AAAAATAAAT 
GTAATATCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

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LOC552814 cDNA ORF clone, Apis mellifera(honey bee)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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