LOC5571389 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5571389
Entrez Gene ID	5571389
Full Name	mediator of RNA polymerase II transcription subunit 24
Synonyms	AAEL009027,MED24
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001653591.1	XP_001653641.1	AAEL009027-PA
XM_001653591.2	XP_001653641.1	mediator of RNA polymerase II transcription subunit 24
XM_021841712.1	XP_021697404.1	mediator of RNA polymerase II transcription subunit 24

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5571389 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5571389 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe06834	XM_001653591.1 Latest version!	Aedes aegypti AAEL009027-RA (MED24_AEDAE), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe119730	XM_021841712.1 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 24 (LOC5571389), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe119730	XM_001653591.2 Latest version!	Aedes aegypti mediator of RNA polymerase II transcription subunit 24 (LOC5571389), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5571389 ( XM_001653591.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001653641.1 Aedes aegypti AAEL009027-RA (MED24_AEDAE), partial mRNA.

Price & Availability↑

CloneID	OAe06834
Clone ID Related Accession (Same CDS sequence)	XM_001653591.1
Accession Version	XM_001653591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL009027-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811089). COMPLETENESS: incomplete on the 3' end.

ATGATCATGG ACAACCAGAA ATCGACCAGC AAAACAACGC TCCTCAAGCT GATGCTGCTG 
CGGGCCTGGA AGGAACGCTG GACCGATTGC CAGTGGGGAA TCAACGTGAA GGCGGTCCTA 
ACACGTGGTG TAAGCGGAGA TGTCTACAAT TTGGCCGATT GCATTCTGCA GCAAGCAGTT 
GTTGGATCCG GCGCCAACAC CTTGTTCCTA TCCTACCTGA AGCACTCGTT GTGCGCCCAC 
CTGATCTCCC ACGCGGCCGT GCTCAAGCGC ATCTCCAAGT ACGATCACTT CGAGATGCAC 
CACTGCTTGA TCGCGTTGCT CGAGTTCCTG GAGTCGATCA TCGGTGGCGT GACGTGCCGC 
GGCAAGCAGG AGGAAGGCCT GCTGACCAAA GCAATGCTCT CGCTGGTCTA CTGGTTGATG 
CAGATCTACG AGCACGCGAT CGAGACGTTC AGCGAGAACC GATCGCTCAC CACCGAACAG 
CAGGAAGTGG TGGAGAAAAC GGCGAATGTG CTGGAGAAGA TCGTGCAGAG TCAGTTCTTG 
TTGGGGGTGG TTTTCGTCGG AAAATTCGAG GACCCGGAAC TGTTTGGAAT GCTGGCGAAG 
AAATGTCTGG AGATCGAGAA TTTGACGGTA AAGTCTGGGT TTGTTCCGCC AGTGGTTAGC 
CAAAAGAATG TAACGGTCAA CGACTACATT CGGAAGGTGG CTAGTATTGA GACGGAAACG 
TTGGAGATTA AGGAATTCGA TGGGAAGGGT GTGGAACCGA TCACCTACTG CATGCAGCCG 
TTGATCGCGA TCGATATTCT GTTCAATCCC AACTGCGACA CCCAGATGTA TGTGCTGGAG 
TTCCTGTCGA TCCAAGCGCT GAAGGGTTTT CCGCTGTCTA GGCTGTACTG CGAGATTATC 
CGCGCTTGCT TGACATGCCT GAACAATGTG AGCGGAACTA GCAGGGAATC GTACATCTGC 
GCGTTCACGT TCATCAAAGT GCCGCAGATT ATGCGTCAGA TTCATCTACA AACGAGAGTG 
CTTACCCCGA ACGACTTTGA AGATGCTAAG CTGGATTACT CGCCGGATGT CGTTGAGGCT 
TTTGAGCTGC TTCTGCAGGA TTCCGCAATC TTGGACTTCA TGGATGCCAA GTGCGCTTGC 
AATACGGTGG AATACTTGCT TAACGAGATG CTCAAGCACC ACTTGATCAA CGAGAAGTAC 
TCAAAGCTGA TCGCAGCCAA GCGGGACGGG ATAACCAGCA GTTTGCAGAA GCTCGATTTG 
AACACTAATC AGCATTCGAT AGTGAAGTTT GTTCTGCGAG CAGAGCCTCC CTTGGTCGGT 
ATTCTGAAGA CCCTCAGCTC CGATTACAAC AAGGTTCAGG AGGCCTTGCT CGGGATGCTT 
TGCCAGGTGC TGTCCGGTAA CAGTTTCGAG CTGATTCTGT CCGTCGCCAC CGTTGAGGGG 
AAACTCAAAA CCTTCGTCGC GGGATTGATC AAATGCAACG AGAATTCCAA GCAGGTCCCC 
GGGGAAATGG GAAAACCTGC GATGACCCGG GCAGCTCTGT TCGACGTGTC CTTCCTGATG 
TTGGTCTTCA TCGTGCAGAA TTACGGTTCG GAGGGGGTCC TGACCGACGG CACGGATTCT 
TTCTTCGAGA AGTGGGTCCG AGAATGCATG GTAGAGAAGC ACAAGTCCAA ATCTCCAATG 
AACATGGTCA AGCTCTGTGA CCAGAATAAG GTCGATGAGC TCATCGTGAG TCTGAATTCT 
CCGGACGGCT TGAAGGCGAC TTCCCTCAAG TGGCAGGAAA TTTGCGCAAA CATTCCCGGT 
CTGCTCTATC AGGTACTTCT CGCGTGGGAA AACGAAACCC TTTCCACGGG GGAGATCAAG 
AAGTTCCTGG ACAGCCTCAA GTCCAGGTTC TGCTGTTTCT CGATTTGTGC CACGTCATGG 
CTGTGCGCAT ACATGCAAAT CATCCGCCAG GATGAACTCC TGAAACCGAT GAATATGATT 
CAACAGTTCG TGACCAACAT CAGCTCCGAG GAGATGATGC AGCAGGAATA CCTGAAGGAA 
CGCCTTGGAC TAACGGTGCA GATCATCCGC AAGATGCAGA TCGACTTCCA ACGACTGCCG 
GTGGCCAGTC CCAAGATGAG AAACCAGCTG CAGTCGCAGA GTTTGGTTTC GCAGGCCCCG 
CTGGAAGAGC AGTTCGAGGA GGTGTGGAAG GGAGTGCTGG AGAAGGGCTG GCTGCCAATC 
GAGGCGACGC TGGCGCTGGA GAATTTGCTG CAGTCTTGCG GGCCGTTCTG GCTGGTGGAT 
AAACTGGTGC AGCAAATTTT CCACTGCAAA TATATCAGGG AGATGAATAA AACTATGGAT 
ATCGTTTTTG CGATCATGCA TTTGGATATA GAACGTTGTA CCATTGCACT GCTCTCTCAG 
CTGGTCCCGA TGATGCTGCT CAACAAACTA CAAATATCGG AAATCGTCGA TCCGTTCTCC 
AGAGTTCTGG CCAAACTGTG CGTCTACTGC ATTGTGGCCA CCATGGAAGC CCCGGCCGTG 
CCCACCAAGA AGCGTTCCCG CGCAGCCATC ACTGCCGAAG GCGACGAACT GGACACGCTC 
TGCTCCACGC CCAAAATGCG CAAGATCGGT TCCGAGTCGT GCGACTCCAG TTCCAGTGAT 
TTCATGCTGG AACAGGTGCT GGCTGCCCGG GACAACCCGG CGCCGTTGAA GGAACCGCTG 
CAGGGCTGCC TGCAGACACT TTTCCGCACC TTTTCCCAGT ACATCGTGTC GGATGAGCTC 
TCGCCGAAGA TCTTCTTCAT GTTCCAGCTG CTGTCGCTGC TGGTCGAGGT GGGCAAGGAC 
CGGATCAAGC CGGTTTTGAA GTTGATCCCC AACGGGCTGA TCCAGAACAT GATGAAGATC 
AACGCCACGG ACGATATGAC CGTTGGGTTC ATTCTAAGGT TATACGACTT AAACACCGCT 
TCCGGTAGGC AGTTTGCCAT GTCGGATCTC TGCCTGTTCC GAAACATTCA GATGCGTAAG 
GATAGTATTA AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001653641.1
CDS	88..3105
Translation	MIMDNQKSTSKTTLLKLMLLRAWKERWTDCQWGINVKAVLTRGVSGDVYNLADCILQQAVVGSGANTLFLSYLKHSLCAHLISHAAVLKRISKYDHFEMHHCLIALLEFLESIIGGVTCRGKQEEGLLTKAMLSLVYWLMQIYEHAIETFSENRSLTTEQQEVVEKTANVLEKIVQSQFLLGVVFVGKFEDPELFGMLAKKCLEIENLTVKSGFVPPVVSQKNVTVNDYIRKVASIETETLEIKEFDGKGVEPITYCMQPLIAIDILFNPNCDTQMYVLEFLSIQALKGFPLSRLYCEIIRACLTCLNNVSGTSRESYICAFTFIKVPQIMRQIHLQTRVLTPNDFEDAKLDYSPDVVEAFELLLQDSAILDFMDAKCACNTVEYLLNEMLKHHLINEKYSKLIAAKRDGITSSLQKLDLNTNQHSIVKFVLRAEPPLVGILKTLSSDYNKVQEALLGMLCQVLSGNSFELILSVATVEGKLKTFVAGLIKCNENSKQVPGEMGKPAMTRAALFDVSFLMLVFIVQNYGSEGVLTDGTDSFFEKWVRECMVEKHKSKSPMNMVKLCDQNKVDELIVSLNSPDGLKATSLKWQEICANIPGLLYQVLLAWENETLSTGEIKKFLDSLKSRFCCFSICATSWLCAYMQIIRQDELLKPMNMIQQFVTNISSEEMMQQEYLKERLGLTVQIIRKMQIDFQRLPVASPKMRNQLQSQSLVSQAPLEEQFEEVWKGVLEKGWLPIEATLALENLLQSCGPFWLVDKLVQQIFHCKYIREMNKTMDIVFAIMHLDIERCTIALLSQLVPMMLLNKLQISEIVDPFSRVLAKLCVYCIVATMEAPAVPTKKRSRAAITAEGDELDTLCSTPKMRKIGSESCDSSSSDFMLEQVLAARDNPAPLKEPLQGCLQTLFRTFSQYIVSDELSPKIFFMFQLLSLLVEVGKDRIKPVLKLIPNGLIQNMMKINATDDMTVGFILRLYDLNTASGRQFAMSDLCLFRNIQMRKDSIKL

Target ORF information:

RefSeq Version	XM_001653591.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL009027-RA (MED24_AEDAE), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001653591.1

ORF Insert Sequence:

ATGATCATGG ACAACCAGAA ATCGACCAGC AAAACAACGC TCCTCAAGCT GATGCTGCTG 
CGGGCCTGGA AGGAACGCTG GACCGATTGC CAGTGGGGAA TCAACGTGAA GGCGGTCCTA 
ACACGTGGTG TAAGCGGAGA TGTCTACAAT TTGGCCGATT GCATTCTGCA GCAAGCAGTT 
GTTGGATCCG GCGCCAACAC CTTGTTCCTA TCCTACCTGA AGCACTCGTT GTGCGCCCAC 
CTGATCTCCC ACGCGGCCGT GCTCAAGCGC ATCTCCAAGT ACGATCACTT CGAGATGCAC 
CACTGCTTGA TCGCGTTGCT CGAGTTCCTG GAGTCGATCA TCGGTGGCGT GACGTGCCGC 
GGCAAGCAGG AGGAAGGCCT GCTGACCAAA GCAATGCTCT CGCTGGTCTA CTGGTTGATG 
CAGATCTACG AGCACGCGAT CGAGACGTTC AGCGAGAACC GATCGCTCAC CACCGAACAG 
CAGGAAGTGG TGGAGAAAAC GGCGAATGTG CTGGAGAAGA TCGTGCAGAG TCAGTTCTTG 
TTGGGGGTGG TTTTCGTCGG AAAATTCGAG GACCCGGAAC TGTTTGGAAT GCTGGCGAAG 
AAATGTCTGG AGATCGAGAA TTTGACGGTA AAGTCTGGGT TTGTTCCGCC AGTGGTTAGC 
CAAAAGAATG TAACGGTCAA CGACTACATT CGGAAGGTGG CTAGTATTGA GACGGAAACG 
TTGGAGATTA AGGAATTCGA TGGGAAGGGT GTGGAACCGA TCACCTACTG CATGCAGCCG 
TTGATCGCGA TCGATATTCT GTTCAATCCC AACTGCGACA CCCAGATGTA TGTGCTGGAG 
TTCCTGTCGA TCCAAGCGCT GAAGGGTTTT CCGCTGTCTA GGCTGTACTG CGAGATTATC 
CGCGCTTGCT TGACATGCCT GAACAATGTG AGCGGAACTA GCAGGGAATC GTACATCTGC 
GCGTTCACGT TCATCAAAGT GCCGCAGATT ATGCGTCAGA TTCATCTACA AACGAGAGTG 
CTTACCCCGA ACGACTTTGA AGATGCTAAG CTGGATTACT CGCCGGATGT CGTTGAGGCT 
TTTGAGCTGC TTCTGCAGGA TTCCGCAATC TTGGACTTCA TGGATGCCAA GTGCGCTTGC 
AATACGGTGG AATACTTGCT TAACGAGATG CTCAAGCACC ACTTGATCAA CGAGAAGTAC 
TCAAAGCTGA TCGCAGCCAA GCGGGACGGG ATAACCAGCA GTTTGCAGAA GCTCGATTTG 
AACACTAATC AGCATTCGAT AGTGAAGTTT GTTCTGCGAG CAGAGCCTCC CTTGGTCGGT 
ATTCTGAAGA CCCTCAGCTC CGATTACAAC AAGGTTCAGG AGGCCTTGCT CGGGATGCTT 
TGCCAGGTGC TGTCCGGTAA CAGTTTCGAG CTGATTCTGT CCGTCGCCAC CGTTGAGGGG 
AAACTCAAAA CCTTCGTCGC GGGATTGATC AAATGCAACG AGAATTCCAA GCAGGTCCCC 
GGGGAAATGG GAAAACCTGC GATGACCCGG GCAGCTCTGT TCGACGTGTC CTTCCTGATG 
TTGGTCTTCA TCGTGCAGAA TTACGGTTCG GAGGGGGTCC TGACCGACGG CACGGATTCT 
TTCTTCGAGA AGTGGGTCCG AGAATGCATG GTAGAGAAGC ACAAGTCCAA ATCTCCAATG 
AACATGGTCA AGCTCTGTGA CCAGAATAAG GTCGATGAGC TCATCGTGAG TCTGAATTCT 
CCGGACGGCT TGAAGGCGAC TTCCCTCAAG TGGCAGGAAA TTTGCGCAAA CATTCCCGGT 
CTGCTCTATC AGGTACTTCT CGCGTGGGAA AACGAAACCC TTTCCACGGG GGAGATCAAG 
AAGTTCCTGG ACAGCCTCAA GTCCAGGTTC TGCTGTTTCT CGATTTGTGC CACGTCATGG 
CTGTGCGCAT ACATGCAAAT CATCCGCCAG GATGAACTCC TGAAACCGAT GAATATGATT 
CAACAGTTCG TGACCAACAT CAGCTCCGAG GAGATGATGC AGCAGGAATA CCTGAAGGAA 
CGCCTTGGAC TAACGGTGCA GATCATCCGC AAGATGCAGA TCGACTTCCA ACGACTGCCG 
GTGGCCAGTC CCAAGATGAG AAACCAGCTG CAGTCGCAGA GTTTGGTTTC GCAGGCCCCG 
CTGGAAGAGC AGTTCGAGGA GGTGTGGAAG GGAGTGCTGG AGAAGGGCTG GCTGCCAATC 
GAGGCGACGC TGGCGCTGGA GAATTTGCTG CAGTCTTGCG GGCCGTTCTG GCTGGTGGAT 
AAACTGGTGC AGCAAATTTT CCACTGCAAA TATATCAGGG AGATGAATAA AACTATGGAT 
ATCGTTTTTG CGATCATGCA TTTGGATATA GAACGTTGTA CCATTGCACT GCTCTCTCAG 
CTGGTCCCGA TGATGCTGCT CAACAAACTA CAAATATCGG AAATCGTCGA TCCGTTCTCC 
AGAGTTCTGG CCAAACTGTG CGTCTACTGC ATTGTGGCCA CCATGGAAGC CCCGGCCGTG 
CCCACCAAGA AGCGTTCCCG CGCAGCCATC ACTGCCGAAG GCGACGAACT GGACACGCTC 
TGCTCCACGC CCAAAATGCG CAAGATCGGT TCCGAGTCGT GCGACTCCAG TTCCAGTGAT 
TTCATGCTGG AACAGGTGCT GGCTGCCCGG GACAACCCGG CGCCGTTGAA GGAACCGCTG 
CAGGGCTGCC TGCAGACACT TTTCCGCACC TTTTCCCAGT ACATCGTGTC GGATGAGCTC 
TCGCCGAAGA TCTTCTTCAT GTTCCAGCTG CTGTCGCTGC TGGTCGAGGT GGGCAAGGAC 
CGGATCAAGC CGGTTTTGAA GTTGATCCCC AACGGGCTGA TCCAGAACAT GATGAAGATC 
AACGCCACGG ACGATATGAC CGTTGGGTTC ATTCTAAGGT TATACGACTT AAACACCGCT 
TCCGGTAGGC AGTTTGCCAT GTCGGATCTC TGCCTGTTCC GAAACATTCA GATGCGTAAG 
GATAGTATTA AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5571389 ( XM_001653591.2 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001653641.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 24 (LOC5571389), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAe119730
Clone ID Related Accession (Same CDS sequence)	XM_001653591.2 , XM_021841712.1
Accession Version	XM_001653591.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001653591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCATGG ACAACCAGAA ATCGACCAGC AAAACAACGC TCCTCAAGCT GATGCTGCTG 
CGGGCCTGGA AGGAACGCTG GACCGATTGC CAGTGGGGAA TCAACGTGAA GGCGGTCCTA 
ACACGTGGTG TAAGCGGAGA TGTCTACAAT TTGGCCGATT GCATTCTGCA GCAAGCAGTT 
GTTGGATCCG GCGCCAACAC CTTGTTCCTA TCCTACCTGA AGCACTCGTT GTGCGCCCAC 
CTGATCTCCC ACGCGGCCGT GCTCAAGCGC ATCTCCAAGT ACGATCACTT CGAGATGCAC 
CACTGCTTGA TCGCGTTGCT CGAGTTCCTG GAGTCGATCA TCGGTGGCGT GACGTGCCGC 
GGCAAGCAGG AGGAAGGCCT GCTGACCAAA GCAATGCTCT CGCTGGTCTA CTGGTTGATG 
CAGATCTACG AGCACGCGAT CGAGACGTTC AGCGAGAACC GATCGCTCAC CACCGAGCAA 
CAGGAAGTGG TGGAGAAAAC GGCGAATGTG CTGGAGAAGA TCGTGCAGAG TCAGTTCTTG 
TTGGGGGTGG TTTTCGTCGG AAAATTCGAG GACCCGGAAC TGTTTGGAAT GCTGGCGAAG 
AAATGTCTGG AGATCGAGAA TTTGACGGTA AAGTCTGGGT TTGTTCCGCC AGTGGTTAGC 
CAAAAGAATG TAACGGTCAA CGACTACATT CGGAAGGTGG CTAGTATTGA GACGGAAACG 
TTGGAGATTA AGGAATTCGA TGGGAAGGGT GTGGAACCGA TCACCTACTG CATGCAGCCG 
TTGATCGCGA TCGATATTCT GTTCAATCCC AACTGCGACA CCCAGATGTA TGTGCTGGAG 
TTCCTGTCGA TCCAAGCGCT GAAGGGTTTT CCGCTGTCTA GGCTGTACTG CGAGATTATC 
CGCGCTTGCT TGACATGCCT GAACAATGTG AGCGGAACTA GCAGGGAATC GTACATCTGC 
GCGTTCACGT TCATCAAAGT GCCGCAGATT ATGCGTCAGA TTCATCTACA AACGAGAGTG 
CTTACCCCGA ACGACTTTGA AGATGCTAAG CTGGATTACT CGCCGGATGT CGTTGAGGCT 
TTTGAGCTGC TTCTGCAGGA TTCCGCAATC TTGGACTTCA TGGATGCCAA GTGCGCTTGC 
AATACGGTGG AATACTTGCT TAACGAGATG CTCAAGCACC ACTTGATCAA CGAGAAGTAC 
TCAAAGCTGA TCGCAGCCAA GCGGGACGGG ATAACCAGCA GTTTGCAGAA GCTCGATTTG 
AACACTAATC AGCATTCGAT AGTGAAGTTT GTTCTGCGAG CAGAGCCTCC CTTGGTCGGT 
ATTCTGAAGA CCCTCAGCTC CGATTACAAC AAGGTTCAGG AGGCCTTGCT CGGGATGCTT 
TGCCAGGTGC TGTCCGGTAA CAGTTTCGAG CTGATTCTGT CCGTCGCCAC CGTTGAGGGG 
AAACTCAAAA CCTTCGTCGC GGGATTGATC AAATGCAACG AGAATTCCAA GCAGGTCCCC 
GGGGAAATGG GAAAACCTGC GATGACCCGG GCAGCTCTGT TCGACGTGTC CTTCCTGATG 
TTGGTCTTCA TCGTGCAGAA TTACGGTTCG GAGGGGGTCC TGACCGACGG CACGGATTCT 
TTCTTCGAGA AGTGGGTCCG AGAATGCATG GTAGAGAAGC ACAAGTCCAA ATCTCCAATG 
AACATGGTCA AGCTCTGTGA CCAGAATAAG GTCGATGAGC TCATCGTGAG TCTGAATTCT 
CCGGACGGCT TGAAGGCGAC TTCCCTCAAG TGGCAGGAAA TTTGCGCAAA CATTCCCGGT 
CTGCTCTATC AGGTACTTCT CGCGTGGGAA AACGAAACCC TTTCCACGGG GGAGATCAAG 
AAGTTCCTGG ACAGCCTCAA GTCCAGGTTC TGCTGTTTCT CGATTTGTGC CACGTCATGG 
CTGTGCGCAT ACATGCAAAT CATCCGCCAG GATGAACTCC TGAAACCGAT GAATATGATT 
CAACAGTTCG TGACCAACAT CAGCTCCGAG GAGATGATGC AGCAGGAATA CCTGAAGGAA 
CGCCTTGGAC TAACGGTGCA GATCATCCGC AAGATGCAGA TCGACTTCCA ACGACTGCCG 
GTGGCCAGTC CCAAGATGAG AAACCAGCTG CAGTCGCAGA GTTTGGTTTC GCAGGCCCCG 
CTGGAAGAGC AGTTCGAGGA GGTGTGGAAG GGAGTGCTGG AGAAGGGCTG GCTGCCAATC 
GAGGCGACGC TGGCGCTGGA GAATTTGCTG CAGTCTTGCG GGCCGTTCTG GCTGGTGGAT 
AAACTGGTGC AGCAAATTTT CCACTGCAAA TATATCAGGG AGATGAATAA AACTATGGAT 
ATCGTTTTTG CGATCATGCA TTTGGATATA GAACGTTGTA CCATTGCACT GCTCTCTCAG 
CTGGTCCCGA TGATGCTGCT CAACAAACTA CAAATATCGG AAATCGTCGA TCCGTTCTCC 
AGAGTTCTGG CCAAACTGTG CGTCTACTGC ATTGTGGCCA CCATGGAAGC CCCGGCCGTG 
CCCACCAAGA AGCGTTCCCG CGCAGCCATC ACTGCCGAAG GCGACGAACT GGACACGCTC 
TGCTCCACGC CCAAAATGCG CAAGATCGGT TCCGAGTCGT GCGACTCCAG TTCCAGTGAT 
TTCATGCTGG AACAGGTGCT GGCTGCCCGG GACAACCCGG CGCCGTTGAA GGAACCGCTG 
CAGGGCTGCC TGCAGACACT TTTCCGCACC TTTTCCCAGT ACATCGTGTC GGATGAGCTC 
TCGCCGAAGA TCTTCTTCAT GTTCCAGCTG CTGTCGCTGC TGGTCGAGGT GGGCAAGGAC 
CGGATCAAGC CGGTTTTGAA GTTGATCCCC AACGGGCTGA TCCAGAACAT GATGAAGATC 
AACGCCACGG ACGATATGAC CGTTGGGTTC ATTCTAAGGT TATACGACTT AAACACCGCT 
TCCGGTAGGC AGTTTGCCAT GTCGGATCTC TGCCTGTTCC GAAACATTCA GATGCGTAAG 
GATAGTATTA AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001653641.1
CDS	119..3136
Translation	MIMDNQKSTSKTTLLKLMLLRAWKERWTDCQWGINVKAVLTRGVSGDVYNLADCILQQAVVGSGANTLFLSYLKHSLCAHLISHAAVLKRISKYDHFEMHHCLIALLEFLESIIGGVTCRGKQEEGLLTKAMLSLVYWLMQIYEHAIETFSENRSLTTEQQEVVEKTANVLEKIVQSQFLLGVVFVGKFEDPELFGMLAKKCLEIENLTVKSGFVPPVVSQKNVTVNDYIRKVASIETETLEIKEFDGKGVEPITYCMQPLIAIDILFNPNCDTQMYVLEFLSIQALKGFPLSRLYCEIIRACLTCLNNVSGTSRESYICAFTFIKVPQIMRQIHLQTRVLTPNDFEDAKLDYSPDVVEAFELLLQDSAILDFMDAKCACNTVEYLLNEMLKHHLINEKYSKLIAAKRDGITSSLQKLDLNTNQHSIVKFVLRAEPPLVGILKTLSSDYNKVQEALLGMLCQVLSGNSFELILSVATVEGKLKTFVAGLIKCNENSKQVPGEMGKPAMTRAALFDVSFLMLVFIVQNYGSEGVLTDGTDSFFEKWVRECMVEKHKSKSPMNMVKLCDQNKVDELIVSLNSPDGLKATSLKWQEICANIPGLLYQVLLAWENETLSTGEIKKFLDSLKSRFCCFSICATSWLCAYMQIIRQDELLKPMNMIQQFVTNISSEEMMQQEYLKERLGLTVQIIRKMQIDFQRLPVASPKMRNQLQSQSLVSQAPLEEQFEEVWKGVLEKGWLPIEATLALENLLQSCGPFWLVDKLVQQIFHCKYIREMNKTMDIVFAIMHLDIERCTIALLSQLVPMMLLNKLQISEIVDPFSRVLAKLCVYCIVATMEAPAVPTKKRSRAAITAEGDELDTLCSTPKMRKIGSESCDSSSSDFMLEQVLAARDNPAPLKEPLQGCLQTLFRTFSQYIVSDELSPKIFFMFQLLSLLVEVGKDRIKPVLKLIPNGLIQNMMKINATDDMTVGFILRLYDLNTASGRQFAMSDLCLFRNIQMRKDSIKL

Target ORF information:

RefSeq Version	XM_001653591.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 24 (LOC5571389), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001653591.2

ORF Insert Sequence:

ATGATCATGG ACAACCAGAA ATCGACCAGC AAAACAACGC TCCTCAAGCT GATGCTGCTG 
CGGGCCTGGA AGGAACGCTG GACCGATTGC CAGTGGGGAA TCAACGTGAA GGCGGTCCTA 
ACACGTGGTG TAAGCGGAGA TGTCTACAAT TTGGCCGATT GCATTCTGCA GCAAGCAGTT 
GTTGGATCCG GCGCCAACAC CTTGTTCCTA TCCTACCTGA AGCACTCGTT GTGCGCCCAC 
CTGATCTCCC ACGCGGCCGT GCTCAAGCGC ATCTCCAAGT ACGATCACTT CGAGATGCAC 
CACTGCTTGA TCGCGTTGCT CGAGTTCCTG GAGTCGATCA TCGGTGGCGT GACGTGCCGC 
GGCAAGCAGG AGGAAGGCCT GCTGACCAAA GCAATGCTCT CGCTGGTCTA CTGGTTGATG 
CAGATCTACG AGCACGCGAT CGAGACGTTC AGCGAGAACC GATCGCTCAC CACCGAGCAA 
CAGGAAGTGG TGGAGAAAAC GGCGAATGTG CTGGAGAAGA TCGTGCAGAG TCAGTTCTTG 
TTGGGGGTGG TTTTCGTCGG AAAATTCGAG GACCCGGAAC TGTTTGGAAT GCTGGCGAAG 
AAATGTCTGG AGATCGAGAA TTTGACGGTA AAGTCTGGGT TTGTTCCGCC AGTGGTTAGC 
CAAAAGAATG TAACGGTCAA CGACTACATT CGGAAGGTGG CTAGTATTGA GACGGAAACG 
TTGGAGATTA AGGAATTCGA TGGGAAGGGT GTGGAACCGA TCACCTACTG CATGCAGCCG 
TTGATCGCGA TCGATATTCT GTTCAATCCC AACTGCGACA CCCAGATGTA TGTGCTGGAG 
TTCCTGTCGA TCCAAGCGCT GAAGGGTTTT CCGCTGTCTA GGCTGTACTG CGAGATTATC 
CGCGCTTGCT TGACATGCCT GAACAATGTG AGCGGAACTA GCAGGGAATC GTACATCTGC 
GCGTTCACGT TCATCAAAGT GCCGCAGATT ATGCGTCAGA TTCATCTACA AACGAGAGTG 
CTTACCCCGA ACGACTTTGA AGATGCTAAG CTGGATTACT CGCCGGATGT CGTTGAGGCT 
TTTGAGCTGC TTCTGCAGGA TTCCGCAATC TTGGACTTCA TGGATGCCAA GTGCGCTTGC 
AATACGGTGG AATACTTGCT TAACGAGATG CTCAAGCACC ACTTGATCAA CGAGAAGTAC 
TCAAAGCTGA TCGCAGCCAA GCGGGACGGG ATAACCAGCA GTTTGCAGAA GCTCGATTTG 
AACACTAATC AGCATTCGAT AGTGAAGTTT GTTCTGCGAG CAGAGCCTCC CTTGGTCGGT 
ATTCTGAAGA CCCTCAGCTC CGATTACAAC AAGGTTCAGG AGGCCTTGCT CGGGATGCTT 
TGCCAGGTGC TGTCCGGTAA CAGTTTCGAG CTGATTCTGT CCGTCGCCAC CGTTGAGGGG 
AAACTCAAAA CCTTCGTCGC GGGATTGATC AAATGCAACG AGAATTCCAA GCAGGTCCCC 
GGGGAAATGG GAAAACCTGC GATGACCCGG GCAGCTCTGT TCGACGTGTC CTTCCTGATG 
TTGGTCTTCA TCGTGCAGAA TTACGGTTCG GAGGGGGTCC TGACCGACGG CACGGATTCT 
TTCTTCGAGA AGTGGGTCCG AGAATGCATG GTAGAGAAGC ACAAGTCCAA ATCTCCAATG 
AACATGGTCA AGCTCTGTGA CCAGAATAAG GTCGATGAGC TCATCGTGAG TCTGAATTCT 
CCGGACGGCT TGAAGGCGAC TTCCCTCAAG TGGCAGGAAA TTTGCGCAAA CATTCCCGGT 
CTGCTCTATC AGGTACTTCT CGCGTGGGAA AACGAAACCC TTTCCACGGG GGAGATCAAG 
AAGTTCCTGG ACAGCCTCAA GTCCAGGTTC TGCTGTTTCT CGATTTGTGC CACGTCATGG 
CTGTGCGCAT ACATGCAAAT CATCCGCCAG GATGAACTCC TGAAACCGAT GAATATGATT 
CAACAGTTCG TGACCAACAT CAGCTCCGAG GAGATGATGC AGCAGGAATA CCTGAAGGAA 
CGCCTTGGAC TAACGGTGCA GATCATCCGC AAGATGCAGA TCGACTTCCA ACGACTGCCG 
GTGGCCAGTC CCAAGATGAG AAACCAGCTG CAGTCGCAGA GTTTGGTTTC GCAGGCCCCG 
CTGGAAGAGC AGTTCGAGGA GGTGTGGAAG GGAGTGCTGG AGAAGGGCTG GCTGCCAATC 
GAGGCGACGC TGGCGCTGGA GAATTTGCTG CAGTCTTGCG GGCCGTTCTG GCTGGTGGAT 
AAACTGGTGC AGCAAATTTT CCACTGCAAA TATATCAGGG AGATGAATAA AACTATGGAT 
ATCGTTTTTG CGATCATGCA TTTGGATATA GAACGTTGTA CCATTGCACT GCTCTCTCAG 
CTGGTCCCGA TGATGCTGCT CAACAAACTA CAAATATCGG AAATCGTCGA TCCGTTCTCC 
AGAGTTCTGG CCAAACTGTG CGTCTACTGC ATTGTGGCCA CCATGGAAGC CCCGGCCGTG 
CCCACCAAGA AGCGTTCCCG CGCAGCCATC ACTGCCGAAG GCGACGAACT GGACACGCTC 
TGCTCCACGC CCAAAATGCG CAAGATCGGT TCCGAGTCGT GCGACTCCAG TTCCAGTGAT 
TTCATGCTGG AACAGGTGCT GGCTGCCCGG GACAACCCGG CGCCGTTGAA GGAACCGCTG 
CAGGGCTGCC TGCAGACACT TTTCCGCACC TTTTCCCAGT ACATCGTGTC GGATGAGCTC 
TCGCCGAAGA TCTTCTTCAT GTTCCAGCTG CTGTCGCTGC TGGTCGAGGT GGGCAAGGAC 
CGGATCAAGC CGGTTTTGAA GTTGATCCCC AACGGGCTGA TCCAGAACAT GATGAAGATC 
AACGCCACGG ACGATATGAC CGTTGGGTTC ATTCTAAGGT TATACGACTT AAACACCGCT 
TCCGGTAGGC AGTTTGCCAT GTCGGATCTC TGCCTGTTCC GAAACATTCA GATGCGTAAG 
GATAGTATTA AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5571389 ( XM_021841712.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021697404.1 Aedes aegypti mediator of RNA polymerase II transcription subunit 24 (LOC5571389), transcript variant X2, mRNA.

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CloneID	OAe119730
Clone ID Related Accession (Same CDS sequence)	XM_001653591.2 , XM_021841712.1
Accession Version	XM_021841712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	mediator of RNA polymerase II transcription subunit 24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCATGG ACAACCAGAA ATCGACCAGC AAAACAACGC TCCTCAAGCT GATGCTGCTG 
CGGGCCTGGA AGGAACGCTG GACCGATTGC CAGTGGGGAA TCAACGTGAA GGCGGTCCTA 
ACACGTGGTG TAAGCGGAGA TGTCTACAAT TTGGCCGATT GCATTCTGCA GCAAGCAGTT 
GTTGGATCCG GCGCCAACAC CTTGTTCCTA TCCTACCTGA AGCACTCGTT GTGCGCCCAC 
CTGATCTCCC ACGCGGCCGT GCTCAAGCGC ATCTCCAAGT ACGATCACTT CGAGATGCAC 
CACTGCTTGA TCGCGTTGCT CGAGTTCCTG GAGTCGATCA TCGGTGGCGT GACGTGCCGC 
GGCAAGCAGG AGGAAGGCCT GCTGACCAAA GCAATGCTCT CGCTGGTCTA CTGGTTGATG 
CAGATCTACG AGCACGCGAT CGAGACGTTC AGCGAGAACC GATCGCTCAC CACCGAGCAA 
CAGGAAGTGG TGGAGAAAAC GGCGAATGTG CTGGAGAAGA TCGTGCAGAG TCAGTTCTTG 
TTGGGGGTGG TTTTCGTCGG AAAATTCGAG GACCCGGAAC TGTTTGGAAT GCTGGCGAAG 
AAATGTCTGG AGATCGAGAA TTTGACGGTA AAGTCTGGGT TTGTTCCGCC AGTGGTTAGC 
CAAAAGAATG TAACGGTCAA CGACTACATT CGGAAGGTGG CTAGTATTGA GACGGAAACG 
TTGGAGATTA AGGAATTCGA TGGGAAGGGT GTGGAACCGA TCACCTACTG CATGCAGCCG 
TTGATCGCGA TCGATATTCT GTTCAATCCC AACTGCGACA CCCAGATGTA TGTGCTGGAG 
TTCCTGTCGA TCCAAGCGCT GAAGGGTTTT CCGCTGTCTA GGCTGTACTG CGAGATTATC 
CGCGCTTGCT TGACATGCCT GAACAATGTG AGCGGAACTA GCAGGGAATC GTACATCTGC 
GCGTTCACGT TCATCAAAGT GCCGCAGATT ATGCGTCAGA TTCATCTACA AACGAGAGTG 
CTTACCCCGA ACGACTTTGA AGATGCTAAG CTGGATTACT CGCCGGATGT CGTTGAGGCT 
TTTGAGCTGC TTCTGCAGGA TTCCGCAATC TTGGACTTCA TGGATGCCAA GTGCGCTTGC 
AATACGGTGG AATACTTGCT TAACGAGATG CTCAAGCACC ACTTGATCAA CGAGAAGTAC 
TCAAAGCTGA TCGCAGCCAA GCGGGACGGG ATAACCAGCA GTTTGCAGAA GCTCGATTTG 
AACACTAATC AGCATTCGAT AGTGAAGTTT GTTCTGCGAG CAGAGCCTCC CTTGGTCGGT 
ATTCTGAAGA CCCTCAGCTC CGATTACAAC AAGGTTCAGG AGGCCTTGCT CGGGATGCTT 
TGCCAGGTGC TGTCCGGTAA CAGTTTCGAG CTGATTCTGT CCGTCGCCAC CGTTGAGGGG 
AAACTCAAAA CCTTCGTCGC GGGATTGATC AAATGCAACG AGAATTCCAA GCAGGTCCCC 
GGGGAAATGG GAAAACCTGC GATGACCCGG GCAGCTCTGT TCGACGTGTC CTTCCTGATG 
TTGGTCTTCA TCGTGCAGAA TTACGGTTCG GAGGGGGTCC TGACCGACGG CACGGATTCT 
TTCTTCGAGA AGTGGGTCCG AGAATGCATG GTAGAGAAGC ACAAGTCCAA ATCTCCAATG 
AACATGGTCA AGCTCTGTGA CCAGAATAAG GTCGATGAGC TCATCGTGAG TCTGAATTCT 
CCGGACGGCT TGAAGGCGAC TTCCCTCAAG TGGCAGGAAA TTTGCGCAAA CATTCCCGGT 
CTGCTCTATC AGGTACTTCT CGCGTGGGAA AACGAAACCC TTTCCACGGG GGAGATCAAG 
AAGTTCCTGG ACAGCCTCAA GTCCAGGTTC TGCTGTTTCT CGATTTGTGC CACGTCATGG 
CTGTGCGCAT ACATGCAAAT CATCCGCCAG GATGAACTCC TGAAACCGAT GAATATGATT 
CAACAGTTCG TGACCAACAT CAGCTCCGAG GAGATGATGC AGCAGGAATA CCTGAAGGAA 
CGCCTTGGAC TAACGGTGCA GATCATCCGC AAGATGCAGA TCGACTTCCA ACGACTGCCG 
GTGGCCAGTC CCAAGATGAG AAACCAGCTG CAGTCGCAGA GTTTGGTTTC GCAGGCCCCG 
CTGGAAGAGC AGTTCGAGGA GGTGTGGAAG GGAGTGCTGG AGAAGGGCTG GCTGCCAATC 
GAGGCGACGC TGGCGCTGGA GAATTTGCTG CAGTCTTGCG GGCCGTTCTG GCTGGTGGAT 
AAACTGGTGC AGCAAATTTT CCACTGCAAA TATATCAGGG AGATGAATAA AACTATGGAT 
ATCGTTTTTG CGATCATGCA TTTGGATATA GAACGTTGTA CCATTGCACT GCTCTCTCAG 
CTGGTCCCGA TGATGCTGCT CAACAAACTA CAAATATCGG AAATCGTCGA TCCGTTCTCC 
AGAGTTCTGG CCAAACTGTG CGTCTACTGC ATTGTGGCCA CCATGGAAGC CCCGGCCGTG 
CCCACCAAGA AGCGTTCCCG CGCAGCCATC ACTGCCGAAG GCGACGAACT GGACACGCTC 
TGCTCCACGC CCAAAATGCG CAAGATCGGT TCCGAGTCGT GCGACTCCAG TTCCAGTGAT 
TTCATGCTGG AACAGGTGCT GGCTGCCCGG GACAACCCGG CGCCGTTGAA GGAACCGCTG 
CAGGGCTGCC TGCAGACACT TTTCCGCACC TTTTCCCAGT ACATCGTGTC GGATGAGCTC 
TCGCCGAAGA TCTTCTTCAT GTTCCAGCTG CTGTCGCTGC TGGTCGAGGT GGGCAAGGAC 
CGGATCAAGC CGGTTTTGAA GTTGATCCCC AACGGGCTGA TCCAGAACAT GATGAAGATC 
AACGCCACGG ACGATATGAC CGTTGGGTTC ATTCTAAGGT TATACGACTT AAACACCGCT 
TCCGGTAGGC AGTTTGCCAT GTCGGATCTC TGCCTGTTCC GAAACATTCA GATGCGTAAG 
GATAGTATTA AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021697404.1
CDS	114..3131
Translation	MIMDNQKSTSKTTLLKLMLLRAWKERWTDCQWGINVKAVLTRGVSGDVYNLADCILQQAVVGSGANTLFLSYLKHSLCAHLISHAAVLKRISKYDHFEMHHCLIALLEFLESIIGGVTCRGKQEEGLLTKAMLSLVYWLMQIYEHAIETFSENRSLTTEQQEVVEKTANVLEKIVQSQFLLGVVFVGKFEDPELFGMLAKKCLEIENLTVKSGFVPPVVSQKNVTVNDYIRKVASIETETLEIKEFDGKGVEPITYCMQPLIAIDILFNPNCDTQMYVLEFLSIQALKGFPLSRLYCEIIRACLTCLNNVSGTSRESYICAFTFIKVPQIMRQIHLQTRVLTPNDFEDAKLDYSPDVVEAFELLLQDSAILDFMDAKCACNTVEYLLNEMLKHHLINEKYSKLIAAKRDGITSSLQKLDLNTNQHSIVKFVLRAEPPLVGILKTLSSDYNKVQEALLGMLCQVLSGNSFELILSVATVEGKLKTFVAGLIKCNENSKQVPGEMGKPAMTRAALFDVSFLMLVFIVQNYGSEGVLTDGTDSFFEKWVRECMVEKHKSKSPMNMVKLCDQNKVDELIVSLNSPDGLKATSLKWQEICANIPGLLYQVLLAWENETLSTGEIKKFLDSLKSRFCCFSICATSWLCAYMQIIRQDELLKPMNMIQQFVTNISSEEMMQQEYLKERLGLTVQIIRKMQIDFQRLPVASPKMRNQLQSQSLVSQAPLEEQFEEVWKGVLEKGWLPIEATLALENLLQSCGPFWLVDKLVQQIFHCKYIREMNKTMDIVFAIMHLDIERCTIALLSQLVPMMLLNKLQISEIVDPFSRVLAKLCVYCIVATMEAPAVPTKKRSRAAITAEGDELDTLCSTPKMRKIGSESCDSSSSDFMLEQVLAARDNPAPLKEPLQGCLQTLFRTFSQYIVSDELSPKIFFMFQLLSLLVEVGKDRIKPVLKLIPNGLIQNMMKINATDDMTVGFILRLYDLNTASGRQFAMSDLCLFRNIQMRKDSIKL

Target ORF information:

RefSeq Version	XM_021841712.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti mediator of RNA polymerase II transcription subunit 24 (LOC5571389), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021841712.1

ORF Insert Sequence:

ATGATCATGG ACAACCAGAA ATCGACCAGC AAAACAACGC TCCTCAAGCT GATGCTGCTG 
CGGGCCTGGA AGGAACGCTG GACCGATTGC CAGTGGGGAA TCAACGTGAA GGCGGTCCTA 
ACACGTGGTG TAAGCGGAGA TGTCTACAAT TTGGCCGATT GCATTCTGCA GCAAGCAGTT 
GTTGGATCCG GCGCCAACAC CTTGTTCCTA TCCTACCTGA AGCACTCGTT GTGCGCCCAC 
CTGATCTCCC ACGCGGCCGT GCTCAAGCGC ATCTCCAAGT ACGATCACTT CGAGATGCAC 
CACTGCTTGA TCGCGTTGCT CGAGTTCCTG GAGTCGATCA TCGGTGGCGT GACGTGCCGC 
GGCAAGCAGG AGGAAGGCCT GCTGACCAAA GCAATGCTCT CGCTGGTCTA CTGGTTGATG 
CAGATCTACG AGCACGCGAT CGAGACGTTC AGCGAGAACC GATCGCTCAC CACCGAGCAA 
CAGGAAGTGG TGGAGAAAAC GGCGAATGTG CTGGAGAAGA TCGTGCAGAG TCAGTTCTTG 
TTGGGGGTGG TTTTCGTCGG AAAATTCGAG GACCCGGAAC TGTTTGGAAT GCTGGCGAAG 
AAATGTCTGG AGATCGAGAA TTTGACGGTA AAGTCTGGGT TTGTTCCGCC AGTGGTTAGC 
CAAAAGAATG TAACGGTCAA CGACTACATT CGGAAGGTGG CTAGTATTGA GACGGAAACG 
TTGGAGATTA AGGAATTCGA TGGGAAGGGT GTGGAACCGA TCACCTACTG CATGCAGCCG 
TTGATCGCGA TCGATATTCT GTTCAATCCC AACTGCGACA CCCAGATGTA TGTGCTGGAG 
TTCCTGTCGA TCCAAGCGCT GAAGGGTTTT CCGCTGTCTA GGCTGTACTG CGAGATTATC 
CGCGCTTGCT TGACATGCCT GAACAATGTG AGCGGAACTA GCAGGGAATC GTACATCTGC 
GCGTTCACGT TCATCAAAGT GCCGCAGATT ATGCGTCAGA TTCATCTACA AACGAGAGTG 
CTTACCCCGA ACGACTTTGA AGATGCTAAG CTGGATTACT CGCCGGATGT CGTTGAGGCT 
TTTGAGCTGC TTCTGCAGGA TTCCGCAATC TTGGACTTCA TGGATGCCAA GTGCGCTTGC 
AATACGGTGG AATACTTGCT TAACGAGATG CTCAAGCACC ACTTGATCAA CGAGAAGTAC 
TCAAAGCTGA TCGCAGCCAA GCGGGACGGG ATAACCAGCA GTTTGCAGAA GCTCGATTTG 
AACACTAATC AGCATTCGAT AGTGAAGTTT GTTCTGCGAG CAGAGCCTCC CTTGGTCGGT 
ATTCTGAAGA CCCTCAGCTC CGATTACAAC AAGGTTCAGG AGGCCTTGCT CGGGATGCTT 
TGCCAGGTGC TGTCCGGTAA CAGTTTCGAG CTGATTCTGT CCGTCGCCAC CGTTGAGGGG 
AAACTCAAAA CCTTCGTCGC GGGATTGATC AAATGCAACG AGAATTCCAA GCAGGTCCCC 
GGGGAAATGG GAAAACCTGC GATGACCCGG GCAGCTCTGT TCGACGTGTC CTTCCTGATG 
TTGGTCTTCA TCGTGCAGAA TTACGGTTCG GAGGGGGTCC TGACCGACGG CACGGATTCT 
TTCTTCGAGA AGTGGGTCCG AGAATGCATG GTAGAGAAGC ACAAGTCCAA ATCTCCAATG 
AACATGGTCA AGCTCTGTGA CCAGAATAAG GTCGATGAGC TCATCGTGAG TCTGAATTCT 
CCGGACGGCT TGAAGGCGAC TTCCCTCAAG TGGCAGGAAA TTTGCGCAAA CATTCCCGGT 
CTGCTCTATC AGGTACTTCT CGCGTGGGAA AACGAAACCC TTTCCACGGG GGAGATCAAG 
AAGTTCCTGG ACAGCCTCAA GTCCAGGTTC TGCTGTTTCT CGATTTGTGC CACGTCATGG 
CTGTGCGCAT ACATGCAAAT CATCCGCCAG GATGAACTCC TGAAACCGAT GAATATGATT 
CAACAGTTCG TGACCAACAT CAGCTCCGAG GAGATGATGC AGCAGGAATA CCTGAAGGAA 
CGCCTTGGAC TAACGGTGCA GATCATCCGC AAGATGCAGA TCGACTTCCA ACGACTGCCG 
GTGGCCAGTC CCAAGATGAG AAACCAGCTG CAGTCGCAGA GTTTGGTTTC GCAGGCCCCG 
CTGGAAGAGC AGTTCGAGGA GGTGTGGAAG GGAGTGCTGG AGAAGGGCTG GCTGCCAATC 
GAGGCGACGC TGGCGCTGGA GAATTTGCTG CAGTCTTGCG GGCCGTTCTG GCTGGTGGAT 
AAACTGGTGC AGCAAATTTT CCACTGCAAA TATATCAGGG AGATGAATAA AACTATGGAT 
ATCGTTTTTG CGATCATGCA TTTGGATATA GAACGTTGTA CCATTGCACT GCTCTCTCAG 
CTGGTCCCGA TGATGCTGCT CAACAAACTA CAAATATCGG AAATCGTCGA TCCGTTCTCC 
AGAGTTCTGG CCAAACTGTG CGTCTACTGC ATTGTGGCCA CCATGGAAGC CCCGGCCGTG 
CCCACCAAGA AGCGTTCCCG CGCAGCCATC ACTGCCGAAG GCGACGAACT GGACACGCTC 
TGCTCCACGC CCAAAATGCG CAAGATCGGT TCCGAGTCGT GCGACTCCAG TTCCAGTGAT 
TTCATGCTGG AACAGGTGCT GGCTGCCCGG GACAACCCGG CGCCGTTGAA GGAACCGCTG 
CAGGGCTGCC TGCAGACACT TTTCCGCACC TTTTCCCAGT ACATCGTGTC GGATGAGCTC 
TCGCCGAAGA TCTTCTTCAT GTTCCAGCTG CTGTCGCTGC TGGTCGAGGT GGGCAAGGAC 
CGGATCAAGC CGGTTTTGAA GTTGATCCCC AACGGGCTGA TCCAGAACAT GATGAAGATC 
AACGCCACGG ACGATATGAC CGTTGGGTTC ATTCTAAGGT TATACGACTT AAACACCGCT 
TCCGGTAGGC AGTTTGCCAT GTCGGATCTC TGCCTGTTCC GAAACATTCA GATGCGTAAG 
GATAGTATTA AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5571389 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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