AAEL_AAEL008178 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL008178
Entrez Gene ID	5570235
Full Name	probable DNA helicase MCM9
Synonyms	AAEL008178
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001658964.2	XP_001659014.2	probable DNA helicase MCM9
XM_001658964.1	XP_001659014.1	AAEL008178-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL008178 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL008178 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAe05821	XM_001658964.1 Latest version!	Aedes aegypti AAEL008178-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe126920	XM_001658964.2 Latest version!	Aedes aegypti probable DNA helicase MCM9 (LOC5570235), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL008178 ( XM_001658964.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001659014.1 Aedes aegypti AAEL008178-RA partial mRNA.

Price & Availability↑

CloneID	OAe05821
Clone ID Related Accession (Same CDS sequence)	XM_001658964.1
Accession Version	XM_001658964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL008178-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811024). COMPLETENESS: incomplete on the 5' end.

ATGGAGGACT ACTTGAAACA GTACTACGAT GAAGAAATCA CAAATCTGTT GAATAACCCC 
GATGACATGG TTAACGTTTC GATTAATGTC AATCTATCTC ACCTTCAGCG AAAGCAACCT 
GCCTTGTACA ATTCATTGCT TCGAAATTAT CGCTCCGAAT GGATCAAATG GAACACCTGC 
CTGCTGAACG CTCAGAAGTC CCTCGTGGAA GGGAACATGT TCCTCGAGCC CGGCTTCCAA 
ATCAAGCAAA ACTGCCACGT TCGGTTCGTC AACATGCCCC AAACGGTGGC CGAGGCCGTT 
CGGAAGGTGG CATTCCCCAA CAACGACAAC GTCGGTCAGT TTGTGCAGGT CAAGGGCAGC 
GTGATCCGAA TGACCCAGGC AAGGTTCTTG GAGTTCAAAA AGGAGTATAC TTGTAGCAGA 
TGTAAGAATG ATTTCACACT GGAAGCGCAG TACGAGAAGA GCTATGTCTT TGACCCGCCT 
CGTGCTTGTC CGCTAGCTGG TGAAACTGGT TGCAAGGGAA CTCCGCATCA GAAGAGTGCC 
CAACCTCAGC CGGATCACTG CCGGGATTAT CAGGAAATAC GCATCCAGGA GATTATGAGC 
GAACGAAACG TCCCGGCCTC GTTGTTGGTA ACGCTGGAGA ATGACTTGGT GGACAATTGC 
CAGCCCGGGG ATTGCGTGAC CGTGGTGGGA ATCATAGAGC GCCGTTGGAG TCCGCTGGTT 
CAAGGGAAGC GAACGGAGGT GACCATAGCT ATGAATGCCA ACAGCGTTTC CAAGGACGAG 
AACAAGATGA ACCTGGGGAA GGATCTGCCG GAGCATTTGG TGTTTGTGCG AGGGGAATGG 
CAGAATACGA TCAAGGAGAT CGGGGAACTG GCTGCGAGGG ACATGCTTGT GCAGTCATTC 
TGCCCCGAGA TCCACGGGAT GTATCCGGTG AAGCTGGCGG TTGCTCTTTC ACTCGCAAGC 
TGTACGGAGA GATTCCTCGG TAGTGGGGCA AGCGTGCGAG GACACTCCCA TCTACTGTTG 
GTCGGAGATC CAGGACTGGC GAAGTCACGG CTGCTGAAGT TCGCTTCGGA AGTTTCGGTG 
CGATCGGTTT TCACCACCGG AATGGGATGT TCCGCTGCAG GGTTGACGGC AGCGGCCGTG 
AAAGAGGACG GCGAGTGGCA ACTGGAGGCC GGGGCGTTGG TCCTTGCAGA CGGTGGAGTC 
TGCTGCATCG ATGAGTTCAA TCTGATGCGT GAATCGGACA AGGCATCGAT CCACGAGGCG 
ATGGAACAGC AAACGATCAG CATGGCAAAG GCCGGACTGG TCTGCAAGTT GAGTACCAGG 
TGCGTCGTCC TGGCCGCAAC CAATCCCAAG AATTTACTAT CCATGGTCGA GATGGAGGCC 
AACAGTAGTG CCAGCCTGGG CATTGGGGGT CCGCTGCTGA GTCGATTCGA TTTGGTGCTG 
ATTTTGACGG ACGATCGGAA CGAGCACTGG GATGAGCGGG TGGCCAACCA CATCCTGGCG 
CTCTCGGTTG TGGACGAGAC AAGGGACAAG TTTGAGGAAA CTACACCGGA CGGGCACTGG 
GATTTGGAAC GTCTGCAGAC GCATTTTCTG GCGATTAAGG ACATTCATCC TCGGATTACG 
GACGACGCCA ATACCATTTT GGGGGCGTAC TACAAACTAT GTCGCTCGGA TCCGTCACGT 
GACCCGTCGC GAACCACCGT TCGATTGCTG GACAGTTTGG TCCGCCTCTC GCAGGCCCAT 
GCTCGTCTGC TGTTCCGAGA TGAGGTCAAC CCGGTGGATG CAATCACCGT CATCCGGTTG 
ATGGAATCGA CTTGGGGCTT TGGAAAGGTT TTACAGCCGA CCGATTTGAT ACGCAAAGGA 
CTTCCCATAG GTCCAACTCC GCAGGAGATT GCTGAACTTC TTGAACAACT ACTTCTGTCT 
GATGTAGTGA AAGATATTCA GCTGGAACAG GTCAATAAAG GTCGTGTCGA ACGGTATCAG 
CAGGAATTGG CGAAGAAGAA GAAGGAAGCT ACGCCCGCAA ATAAATGGGA ATCTTTGTCG 
ACGCAAAGAA TAATCGAGTT CGACGAGAGA GTCCTCAGTG CGTTGGAGAA CAATCGAAGT 
GAGAGCAGTA AGCAAAAGGA AGTTATTGAA GAGCCTAATC CTGAAGAGCC GAAGCAAAAA 
CCAATTGAAG AAAGAGAACT CTATTCGAAA TACTCATTTA CGCAAATGCG TAAGAACAAC 
GTGAAGAAGC TACGTAAGGA AAGTTCGGAG AAGACTGTGG ACCCCAAGCC ACCCGAACCT 
GCAAAGAAAT CAAAAACGAA AAAACCTTTC CCCGAACCAG ATCCAAAACT AAAAGAAAAC 
TTGAACAATG ATCAGCTGGA TCGTATCTTT AGTAGCATAC GCCAAAACTT CATGTCCGAG 
CTGAGTGGTG TAACTCAACA AACGAAAACT AACGAAGGCT CTCAAGAAAG CGTTACGCGA 
AGCAGCCAGA TCGATACGAA TTTTCCGCCA GCCAGTGGAA GATCTAGGGA GGCCCGCGAG 
AAGTCCTTCC GTACCATGCT GGACGTAAGT GGCTTGCTGG ATGACGATCC TCCGGAAGAG 
CGTCAACCGG TCAGTCCCAA AACAACCAAT TCTAGCATGA GTGTGTTTGA GAATGTGGAT 
TCGAGTATAA CGCCGGTGCA ATCCATTGAC AATCCAACGC ATTCTCTACT GACAAAACCG 
CTGGTGAATA CCTCAGATGA CGAATCTCGA TTCCGTCTCA AACGGCCTCG ACACGAGATC 
AAACCAGCCT CCCACAATGA GTCGACGTCG TCCACGGTAT CACGTTTCCA GTTCAAGCGA 
CCAGCGCCAA ACAGCACGCC TTGCAGTCCT AAACCTAGCA ACTCGACGAC ATCGTCAGCA 
GACGATGAAT CCCGGTATCG TTTGAAACGA CCTGCGCCCA AAACTCAAAC CAATGCGTCC 
AACAAACCGA CCACACCGAA CCCGATGCTG ATCAGCCAGC TCAGTTTCGA CATGTCCTTC 
GATACGGAGG CTCTCGCTCT GGAAGAAGCA CCGCAACAGC CCAAGCCGAA GCCATCACTA 
TCCGAACAAA CCTTGAACAA AATGCGGGTC TTTGAGTTCA ACCGGGGTTC GGATGACGGT 
GCTTCGAATT CCAAAGGTGA TCCAGAAGAG AAGAAGCAGC GTGATGATAG CGCATACGAA 
TCGCAGCCGG CATCGACGTC GTCCGCGTCC ACATCCGGTT CAGGTAGGTA CTGGAAGCGA 
GACTTGAAGT TGCCGGAACT GAGTCACGTG GCCACTCTCG GTGGTTCTGG TGACGTGGAT 
GCTGAACTAG CACTGCTCGA GTCGGTGGAT TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001659014.1
CDS	1..3336
Translation	MEDYLKQYYDEEITNLLNNPDDMVNVSINVNLSHLQRKQPALYNSLLRNYRSEWIKWNTCLLNAQKSLVEGNMFLEPGFQIKQNCHVRFVNMPQTVAEAVRKVAFPNNDNVGQFVQVKGSVIRMTQARFLEFKKEYTCSRCKNDFTLEAQYEKSYVFDPPRACPLAGETGCKGTPHQKSAQPQPDHCRDYQEIRIQEIMSERNVPASLLVTLENDLVDNCQPGDCVTVVGIIERRWSPLVQGKRTEVTIAMNANSVSKDENKMNLGKDLPEHLVFVRGEWQNTIKEIGELAARDMLVQSFCPEIHGMYPVKLAVALSLASCTERFLGSGASVRGHSHLLLVGDPGLAKSRLLKFASEVSVRSVFTTGMGCSAAGLTAAAVKEDGEWQLEAGALVLADGGVCCIDEFNLMRESDKASIHEAMEQQTISMAKAGLVCKLSTRCVVLAATNPKNLLSMVEMEANSSASLGIGGPLLSRFDLVLILTDDRNEHWDERVANHILALSVVDETRDKFEETTPDGHWDLERLQTHFLAIKDIHPRITDDANTILGAYYKLCRSDPSRDPSRTTVRLLDSLVRLSQAHARLLFRDEVNPVDAITVIRLMESTWGFGKVLQPTDLIRKGLPIGPTPQEIAELLEQLLLSDVVKDIQLEQVNKGRVERYQQELAKKKKEATPANKWESLSTQRIIEFDERVLSALENNRSESSKQKEVIEEPNPEEPKQKPIEERELYSKYSFTQMRKNNVKKLRKESSEKTVDPKPPEPAKKSKTKKPFPEPDPKLKENLNNDQLDRIFSSIRQNFMSELSGVTQQTKTNEGSQESVTRSSQIDTNFPPASGRSREAREKSFRTMLDVSGLLDDDPPEERQPVSPKTTNSSMSVFENVDSSITPVQSIDNPTHSLLTKPLVNTSDDESRFRLKRPRHEIKPASHNESTSSTVSRFQFKRPAPNSTPCSPKPSNSTTSSADDESRYRLKRPAPKTQTNASNKPTTPNPMLISQLSFDMSFDTEALALEEAPQQPKPKPSLSEQTLNKMRVFEFNRGSDDGASNSKGDPEEKKQRDDSAYESQPASTSSASTSGSGRYWKRDLKLPELSHVATLGGSGDVDAELALLESVDF

Target ORF information:

RefSeq Version	XM_001658964.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL008178-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001658964.1

ORF Insert Sequence:

ATGGAGGACT ACTTGAAACA GTACTACGAT GAAGAAATCA CAAATCTGTT GAATAACCCC 
GATGACATGG TTAACGTTTC GATTAATGTC AATCTATCTC ACCTTCAGCG AAAGCAACCT 
GCCTTGTACA ATTCATTGCT TCGAAATTAT CGCTCCGAAT GGATCAAATG GAACACCTGC 
CTGCTGAACG CTCAGAAGTC CCTCGTGGAA GGGAACATGT TCCTCGAGCC CGGCTTCCAA 
ATCAAGCAAA ACTGCCACGT TCGGTTCGTC AACATGCCCC AAACGGTGGC CGAGGCCGTT 
CGGAAGGTGG CATTCCCCAA CAACGACAAC GTCGGTCAGT TTGTGCAGGT CAAGGGCAGC 
GTGATCCGAA TGACCCAGGC AAGGTTCTTG GAGTTCAAAA AGGAGTATAC TTGTAGCAGA 
TGTAAGAATG ATTTCACACT GGAAGCGCAG TACGAGAAGA GCTATGTCTT TGACCCGCCT 
CGTGCTTGTC CGCTAGCTGG TGAAACTGGT TGCAAGGGAA CTCCGCATCA GAAGAGTGCC 
CAACCTCAGC CGGATCACTG CCGGGATTAT CAGGAAATAC GCATCCAGGA GATTATGAGC 
GAACGAAACG TCCCGGCCTC GTTGTTGGTA ACGCTGGAGA ATGACTTGGT GGACAATTGC 
CAGCCCGGGG ATTGCGTGAC CGTGGTGGGA ATCATAGAGC GCCGTTGGAG TCCGCTGGTT 
CAAGGGAAGC GAACGGAGGT GACCATAGCT ATGAATGCCA ACAGCGTTTC CAAGGACGAG 
AACAAGATGA ACCTGGGGAA GGATCTGCCG GAGCATTTGG TGTTTGTGCG AGGGGAATGG 
CAGAATACGA TCAAGGAGAT CGGGGAACTG GCTGCGAGGG ACATGCTTGT GCAGTCATTC 
TGCCCCGAGA TCCACGGGAT GTATCCGGTG AAGCTGGCGG TTGCTCTTTC ACTCGCAAGC 
TGTACGGAGA GATTCCTCGG TAGTGGGGCA AGCGTGCGAG GACACTCCCA TCTACTGTTG 
GTCGGAGATC CAGGACTGGC GAAGTCACGG CTGCTGAAGT TCGCTTCGGA AGTTTCGGTG 
CGATCGGTTT TCACCACCGG AATGGGATGT TCCGCTGCAG GGTTGACGGC AGCGGCCGTG 
AAAGAGGACG GCGAGTGGCA ACTGGAGGCC GGGGCGTTGG TCCTTGCAGA CGGTGGAGTC 
TGCTGCATCG ATGAGTTCAA TCTGATGCGT GAATCGGACA AGGCATCGAT CCACGAGGCG 
ATGGAACAGC AAACGATCAG CATGGCAAAG GCCGGACTGG TCTGCAAGTT GAGTACCAGG 
TGCGTCGTCC TGGCCGCAAC CAATCCCAAG AATTTACTAT CCATGGTCGA GATGGAGGCC 
AACAGTAGTG CCAGCCTGGG CATTGGGGGT CCGCTGCTGA GTCGATTCGA TTTGGTGCTG 
ATTTTGACGG ACGATCGGAA CGAGCACTGG GATGAGCGGG TGGCCAACCA CATCCTGGCG 
CTCTCGGTTG TGGACGAGAC AAGGGACAAG TTTGAGGAAA CTACACCGGA CGGGCACTGG 
GATTTGGAAC GTCTGCAGAC GCATTTTCTG GCGATTAAGG ACATTCATCC TCGGATTACG 
GACGACGCCA ATACCATTTT GGGGGCGTAC TACAAACTAT GTCGCTCGGA TCCGTCACGT 
GACCCGTCGC GAACCACCGT TCGATTGCTG GACAGTTTGG TCCGCCTCTC GCAGGCCCAT 
GCTCGTCTGC TGTTCCGAGA TGAGGTCAAC CCGGTGGATG CAATCACCGT CATCCGGTTG 
ATGGAATCGA CTTGGGGCTT TGGAAAGGTT TTACAGCCGA CCGATTTGAT ACGCAAAGGA 
CTTCCCATAG GTCCAACTCC GCAGGAGATT GCTGAACTTC TTGAACAACT ACTTCTGTCT 
GATGTAGTGA AAGATATTCA GCTGGAACAG GTCAATAAAG GTCGTGTCGA ACGGTATCAG 
CAGGAATTGG CGAAGAAGAA GAAGGAAGCT ACGCCCGCAA ATAAATGGGA ATCTTTGTCG 
ACGCAAAGAA TAATCGAGTT CGACGAGAGA GTCCTCAGTG CGTTGGAGAA CAATCGAAGT 
GAGAGCAGTA AGCAAAAGGA AGTTATTGAA GAGCCTAATC CTGAAGAGCC GAAGCAAAAA 
CCAATTGAAG AAAGAGAACT CTATTCGAAA TACTCATTTA CGCAAATGCG TAAGAACAAC 
GTGAAGAAGC TACGTAAGGA AAGTTCGGAG AAGACTGTGG ACCCCAAGCC ACCCGAACCT 
GCAAAGAAAT CAAAAACGAA AAAACCTTTC CCCGAACCAG ATCCAAAACT AAAAGAAAAC 
TTGAACAATG ATCAGCTGGA TCGTATCTTT AGTAGCATAC GCCAAAACTT CATGTCCGAG 
CTGAGTGGTG TAACTCAACA AACGAAAACT AACGAAGGCT CTCAAGAAAG CGTTACGCGA 
AGCAGCCAGA TCGATACGAA TTTTCCGCCA GCCAGTGGAA GATCTAGGGA GGCCCGCGAG 
AAGTCCTTCC GTACCATGCT GGACGTAAGT GGCTTGCTGG ATGACGATCC TCCGGAAGAG 
CGTCAACCGG TCAGTCCCAA AACAACCAAT TCTAGCATGA GTGTGTTTGA GAATGTGGAT 
TCGAGTATAA CGCCGGTGCA ATCCATTGAC AATCCAACGC ATTCTCTACT GACAAAACCG 
CTGGTGAATA CCTCAGATGA CGAATCTCGA TTCCGTCTCA AACGGCCTCG ACACGAGATC 
AAACCAGCCT CCCACAATGA GTCGACGTCG TCCACGGTAT CACGTTTCCA GTTCAAGCGA 
CCAGCGCCAA ACAGCACGCC TTGCAGTCCT AAACCTAGCA ACTCGACGAC ATCGTCAGCA 
GACGATGAAT CCCGGTATCG TTTGAAACGA CCTGCGCCCA AAACTCAAAC CAATGCGTCC 
AACAAACCGA CCACACCGAA CCCGATGCTG ATCAGCCAGC TCAGTTTCGA CATGTCCTTC 
GATACGGAGG CTCTCGCTCT GGAAGAAGCA CCGCAACAGC CCAAGCCGAA GCCATCACTA 
TCCGAACAAA CCTTGAACAA AATGCGGGTC TTTGAGTTCA ACCGGGGTTC GGATGACGGT 
GCTTCGAATT CCAAAGGTGA TCCAGAAGAG AAGAAGCAGC GTGATGATAG CGCATACGAA 
TCGCAGCCGG CATCGACGTC GTCCGCGTCC ACATCCGGTT CAGGTAGGTA CTGGAAGCGA 
GACTTGAAGT TGCCGGAACT GAGTCACGTG GCCACTCTCG GTGGTTCTGG TGACGTGGAT 
GCTGAACTAG CACTGCTCGA GTCGGTGGAT TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL008178 ( XM_001658964.2 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001659014.2 Aedes aegypti probable DNA helicase MCM9 (LOC5570235), mRNA.

Price & Availability↑

CloneID	OAe126920
Clone ID Related Accession (Same CDS sequence)	XM_001658964.2
Accession Version	XM_001658964.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	probable DNA helicase MCM9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001658964.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT ACTTGAAACA GTACTACGAT GAAGAAATCA CAAATCTGTT AAATAACCCC 
GATGACATGG TTTACGTTTC GATTAATGTC AATCTAGCCC ACCTTCAGCG AAAGCAACCT 
GCCTTGTACA ATTCATTACT TCGGAACTAT CGCTCGGAAT GGATCAAATG GAACACCTGC 
CTGCTGAACG CTCAGAAGTC CCTCGTGGAA GGGAACATGT TCCTCGAGCC CGGCTTCCAA 
ATCAAGCAAA ACTGCCACGT TCGGTTCGTC AACATGCCCC AAACGGTGGC CGAGGCCGTT 
CGGAAGGTGG CATTCCCCAA CAACGACAAC GTCGGTCAGT TTGTGCAGGT CAAGGGCAGC 
GTGATCCGAA TGACCCAGGC AAGGTTTCTG GAGTTCAAGA AGGAGTATAC TTGTAGCAGA 
TGTAAGAATG ATTTCACACT GGAAGCGCAG TACGAGAAGA GCTATGTCTT TGACCCGCCT 
CGTGCTTGTC CGCTAGCTGG TGAAACTGGT TGCAAGGGAA CTCCGCATCA GAAGAGTGCC 
CAACCTCAGC CGGATCACTG CCGGGATTAT CAGGAAATAC GCATCCAGGA GATTATGAGC 
GAACGAAACG TCCCGGCCTC GTTGTTGGTA ACGCTGGAGA ATGACTTGGT GGACAATTGC 
CAGCCCGGGG ATTGCGTGAC CGTGGTGGGA ATCATAGAGC GCCGTTGGAG TCCGCTGGTT 
CAAGGGAAGC GAACGGAGGT GACCATAGCT ATGAATGCCA ACAGCGTTTC CAAGGACGAG 
AACAAGATGA ACCTGGGGAA GGATCTGCCG GAGCATTTGG TGTTTGTGCG AGGGGAATGG 
CAGAATACGG TCAAGGAGAT CGGGGAACTG GCTGCGAGGG ACATGCTTGT GCAGTCATTC 
TGCCCCGAGA TCCACGGGAT GTATCCGGTG AAGCTGGCGG TTGCTCTTTC ACTCGCAAGC 
TGTACGGAGA GATTCCTCGG TAGTGGGGCA AGCGTGCGAG GACACTCCCA TCTACTGTTG 
GTCGGAGATC CAGGACTGGC CAAGTCACGG CTGCTGAAGT TCGCTTCGGA AGTTTCGGTA 
CGATCGGTTT TCACCACCGG AATGGGATGT TCCGCTGCAG GGTTGACGGC AGCGGCCGTG 
AAAGAGGACG GCGAGTGGCA ACTGGAGGCC GGGGCGTTGG TCCTTGCAGA CGGTGGAGTC 
TGCTGCATCG ATGAGTTCAA TCTGATGCGT GAATCGGACA AGGCATCGAT CCACGAGGCG 
ATGGAACAGC AAACGATCAG CATGGCAAAG GCCGGACTGG TCTGCAAGTT AAGTACCAGG 
TGCGTCGTCC TGGCCGCAAC CAATCCCAAG AATTTACTAT CCATGGTCGA GATGGAGGCC 
AACAGTAGTG CCAGCCTGGG CATTGGGGGT CCGCTGCTGA GTCGGTTCGA TTTGGTGCTG 
ATTTTGACAG ACGATCGGAA CGAGCACTGG GATGAGCGGG TGGCCAATCA CATCCTGGCG 
CTCTCGGTTG TGGACGAGAC AAGGGACAAG TTTGAGGAAA CTACTCCGGA CGGGCACTGG 
GATTTGGAAC GTCTGCAGAC GCACTTTCTG GCGATCAAGG ACATTCACCC GCGGATTACG 
GACGACGCCA ATACCATTTT GGGGGCGTAC TACAAACTAT GTCGCTCGGA TCCGTCACGT 
GACCCGTCGA GAACCACCGT GCGATTGCTG GACAGTTTGG TCCGCCTCTC GCAGGCCCAT 
GCTCGTCTGC TGTTCCGAGA TGAGGTCAAC CCGGTGGATG CAATCACCGT CATCCGGTTG 
ATGGAATCGA CTTGGGGCTT TGGAAAGGTT TTACAGCCCA CCGATTTGAT ACGCAAAGGA 
CTTCCCATAG GTCCAACTCC GCAGGAGATT GCTGAACTTC TTGAACAACT ACTTCTGTCT 
GATGTAGTGA AAGATATTCA GCTGGAACAG GTCAATAAAG GTCGTGTCGA ACGGTATCAG 
CAGGAATTGG CGAAGAAGAA GAAGGAAGCT ACGCCCGCAA ATAAATGGGA ATCTTTGTCG 
ACGCAAAGAA TAATCGAGTT CGACGAGAGA GTCCTCAGTG CGTTGGAGAA CAATCAAAGT 
GAGAGCAGTA AGCAAAAGGA AGTTATTGAA GAGCCTAATT CTGAAGAGCC GAAGCAAAAA 
CCAATTGAAG AAAGAGAACT CTATTCGAAA TACTCATTTA CGCAAATGCG TAAGAACAAC 
GTGAAGAAGC TACGTAAGGA AAGTTCGGAG AAGACTGTGG ACCCCAAGCC ACCCGAACCT 
GCAAAGAAAT CAAAAACGAA AAAACCTTTC CCCGAACCAG ATCCAAAACT AAAAGAAAAC 
ATGAACAATG ATCAGTTGGA TCGCATCTTC AGCAGCATAC GCCAAAACTT CATGTCCGAG 
CTGAGTGGTG CAACTCAACA AACGAAAACT AACGAAGGCT CTCAAGAATG CGTTACGCGA 
AGCAGCCAGA TCGATACCAA TTTTCCGCCA GCCAGCGGAA GATCTAGGGT GTCCCGCGAG 
GAGTCCTTCC GCACCATGCT GGACGTAAGT GGCTTGCTGG ATGACGATCC TCCGGAAGAG 
CGTCAACCGG TCAATCCCAA AACAACCAAT TCTAGCATGA GTGTGTTTGA GAATTTGGAT 
TCGAGTACAA CGCCGGTGCA ATCCATTGAC AATCCAACGC ATTCTCTACT GACAAAACCG 
CTGGTGAATA CCTCAGATGA CGAATCTCGA TTCCGTCTCA AACGGCCTCG AAACGAGATC 
AAACCAGCCT CCCACAATGA GTCGACGTCG TCCACGGTAT CACGTTTCCA GTTCAAGCGA 
CCAGCGCCAA ACAGCACGCC TTGCAGTCCT AAACCTAGCA ACTCGACGAC ATCGTCAGCA 
GACGATGAAT CCCGGTATCG TTTGAAACGA CCTGCGCCCA AAACTCAAAC CAATGCGTCC 
AACAAACCGA CCACACCGAA CCCGATGCTG GTCAGCCAGC TCAGTTTCGA CATGTCCTTC 
GATACGGAGG CTCTCGCTCT GGAAGAAGCA CCGCAACAGC CCAAGCCGAA GCCATCACTA 
TCCGAGCAAA CCTTGAACAA AATGCGGGTC TTTGAGTTTA ACCGGGGTCC GGATGACGGT 
GCTTCAAATT CCAAAGCTGA TCCGGAAGAG AAGAAGCAGC GTGATGATAG CGCATACGAA 
TCGCAGCCGG CATCGTCGTC GTCCGCGTCC ACATCCGGTT CAGGTAGGTA CTGGAAGCGA 
GACTTGAAGT TGCCGGAACT GAGTCACGTG GCCACTCTCG GTGGATCTGG TGACGTGGAT 
GCTGAACTGG CGCTGCTCGA GTCGGTTGAT TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001659014.2
CDS	210..3545
Translation	MEDYLKQYYDEEITNLLNNPDDMVYVSINVNLAHLQRKQPALYNSLLRNYRSEWIKWNTCLLNAQKSLVEGNMFLEPGFQIKQNCHVRFVNMPQTVAEAVRKVAFPNNDNVGQFVQVKGSVIRMTQARFLEFKKEYTCSRCKNDFTLEAQYEKSYVFDPPRACPLAGETGCKGTPHQKSAQPQPDHCRDYQEIRIQEIMSERNVPASLLVTLENDLVDNCQPGDCVTVVGIIERRWSPLVQGKRTEVTIAMNANSVSKDENKMNLGKDLPEHLVFVRGEWQNTVKEIGELAARDMLVQSFCPEIHGMYPVKLAVALSLASCTERFLGSGASVRGHSHLLLVGDPGLAKSRLLKFASEVSVRSVFTTGMGCSAAGLTAAAVKEDGEWQLEAGALVLADGGVCCIDEFNLMRESDKASIHEAMEQQTISMAKAGLVCKLSTRCVVLAATNPKNLLSMVEMEANSSASLGIGGPLLSRFDLVLILTDDRNEHWDERVANHILALSVVDETRDKFEETTPDGHWDLERLQTHFLAIKDIHPRITDDANTILGAYYKLCRSDPSRDPSRTTVRLLDSLVRLSQAHARLLFRDEVNPVDAITVIRLMESTWGFGKVLQPTDLIRKGLPIGPTPQEIAELLEQLLLSDVVKDIQLEQVNKGRVERYQQELAKKKKEATPANKWESLSTQRIIEFDERVLSALENNQSESSKQKEVIEEPNSEEPKQKPIEERELYSKYSFTQMRKNNVKKLRKESSEKTVDPKPPEPAKKSKTKKPFPEPDPKLKENMNNDQLDRIFSSIRQNFMSELSGATQQTKTNEGSQECVTRSSQIDTNFPPASGRSRVSREESFRTMLDVSGLLDDDPPEERQPVNPKTTNSSMSVFENLDSSTTPVQSIDNPTHSLLTKPLVNTSDDESRFRLKRPRNEIKPASHNESTSSTVSRFQFKRPAPNSTPCSPKPSNSTTSSADDESRYRLKRPAPKTQTNASNKPTTPNPMLVSQLSFDMSFDTEALALEEAPQQPKPKPSLSEQTLNKMRVFEFNRGPDDGASNSKADPEEKKQRDDSAYESQPASSSSASTSGSGRYWKRDLKLPELSHVATLGGSGDVDAELALLESVDF

Target ORF information:

RefSeq Version	XM_001658964.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti probable DNA helicase MCM9 (LOC5570235), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001658964.2

ORF Insert Sequence:

ATGGAGGACT ACTTGAAACA GTACTACGAT GAAGAAATCA CAAATCTGTT AAATAACCCC 
GATGACATGG TTTACGTTTC GATTAATGTC AATCTAGCCC ACCTTCAGCG AAAGCAACCT 
GCCTTGTACA ATTCATTACT TCGGAACTAT CGCTCGGAAT GGATCAAATG GAACACCTGC 
CTGCTGAACG CTCAGAAGTC CCTCGTGGAA GGGAACATGT TCCTCGAGCC CGGCTTCCAA 
ATCAAGCAAA ACTGCCACGT TCGGTTCGTC AACATGCCCC AAACGGTGGC CGAGGCCGTT 
CGGAAGGTGG CATTCCCCAA CAACGACAAC GTCGGTCAGT TTGTGCAGGT CAAGGGCAGC 
GTGATCCGAA TGACCCAGGC AAGGTTTCTG GAGTTCAAGA AGGAGTATAC TTGTAGCAGA 
TGTAAGAATG ATTTCACACT GGAAGCGCAG TACGAGAAGA GCTATGTCTT TGACCCGCCT 
CGTGCTTGTC CGCTAGCTGG TGAAACTGGT TGCAAGGGAA CTCCGCATCA GAAGAGTGCC 
CAACCTCAGC CGGATCACTG CCGGGATTAT CAGGAAATAC GCATCCAGGA GATTATGAGC 
GAACGAAACG TCCCGGCCTC GTTGTTGGTA ACGCTGGAGA ATGACTTGGT GGACAATTGC 
CAGCCCGGGG ATTGCGTGAC CGTGGTGGGA ATCATAGAGC GCCGTTGGAG TCCGCTGGTT 
CAAGGGAAGC GAACGGAGGT GACCATAGCT ATGAATGCCA ACAGCGTTTC CAAGGACGAG 
AACAAGATGA ACCTGGGGAA GGATCTGCCG GAGCATTTGG TGTTTGTGCG AGGGGAATGG 
CAGAATACGG TCAAGGAGAT CGGGGAACTG GCTGCGAGGG ACATGCTTGT GCAGTCATTC 
TGCCCCGAGA TCCACGGGAT GTATCCGGTG AAGCTGGCGG TTGCTCTTTC ACTCGCAAGC 
TGTACGGAGA GATTCCTCGG TAGTGGGGCA AGCGTGCGAG GACACTCCCA TCTACTGTTG 
GTCGGAGATC CAGGACTGGC CAAGTCACGG CTGCTGAAGT TCGCTTCGGA AGTTTCGGTA 
CGATCGGTTT TCACCACCGG AATGGGATGT TCCGCTGCAG GGTTGACGGC AGCGGCCGTG 
AAAGAGGACG GCGAGTGGCA ACTGGAGGCC GGGGCGTTGG TCCTTGCAGA CGGTGGAGTC 
TGCTGCATCG ATGAGTTCAA TCTGATGCGT GAATCGGACA AGGCATCGAT CCACGAGGCG 
ATGGAACAGC AAACGATCAG CATGGCAAAG GCCGGACTGG TCTGCAAGTT AAGTACCAGG 
TGCGTCGTCC TGGCCGCAAC CAATCCCAAG AATTTACTAT CCATGGTCGA GATGGAGGCC 
AACAGTAGTG CCAGCCTGGG CATTGGGGGT CCGCTGCTGA GTCGGTTCGA TTTGGTGCTG 
ATTTTGACAG ACGATCGGAA CGAGCACTGG GATGAGCGGG TGGCCAATCA CATCCTGGCG 
CTCTCGGTTG TGGACGAGAC AAGGGACAAG TTTGAGGAAA CTACTCCGGA CGGGCACTGG 
GATTTGGAAC GTCTGCAGAC GCACTTTCTG GCGATCAAGG ACATTCACCC GCGGATTACG 
GACGACGCCA ATACCATTTT GGGGGCGTAC TACAAACTAT GTCGCTCGGA TCCGTCACGT 
GACCCGTCGA GAACCACCGT GCGATTGCTG GACAGTTTGG TCCGCCTCTC GCAGGCCCAT 
GCTCGTCTGC TGTTCCGAGA TGAGGTCAAC CCGGTGGATG CAATCACCGT CATCCGGTTG 
ATGGAATCGA CTTGGGGCTT TGGAAAGGTT TTACAGCCCA CCGATTTGAT ACGCAAAGGA 
CTTCCCATAG GTCCAACTCC GCAGGAGATT GCTGAACTTC TTGAACAACT ACTTCTGTCT 
GATGTAGTGA AAGATATTCA GCTGGAACAG GTCAATAAAG GTCGTGTCGA ACGGTATCAG 
CAGGAATTGG CGAAGAAGAA GAAGGAAGCT ACGCCCGCAA ATAAATGGGA ATCTTTGTCG 
ACGCAAAGAA TAATCGAGTT CGACGAGAGA GTCCTCAGTG CGTTGGAGAA CAATCAAAGT 
GAGAGCAGTA AGCAAAAGGA AGTTATTGAA GAGCCTAATT CTGAAGAGCC GAAGCAAAAA 
CCAATTGAAG AAAGAGAACT CTATTCGAAA TACTCATTTA CGCAAATGCG TAAGAACAAC 
GTGAAGAAGC TACGTAAGGA AAGTTCGGAG AAGACTGTGG ACCCCAAGCC ACCCGAACCT 
GCAAAGAAAT CAAAAACGAA AAAACCTTTC CCCGAACCAG ATCCAAAACT AAAAGAAAAC 
ATGAACAATG ATCAGTTGGA TCGCATCTTC AGCAGCATAC GCCAAAACTT CATGTCCGAG 
CTGAGTGGTG CAACTCAACA AACGAAAACT AACGAAGGCT CTCAAGAATG CGTTACGCGA 
AGCAGCCAGA TCGATACCAA TTTTCCGCCA GCCAGCGGAA GATCTAGGGT GTCCCGCGAG 
GAGTCCTTCC GCACCATGCT GGACGTAAGT GGCTTGCTGG ATGACGATCC TCCGGAAGAG 
CGTCAACCGG TCAATCCCAA AACAACCAAT TCTAGCATGA GTGTGTTTGA GAATTTGGAT 
TCGAGTACAA CGCCGGTGCA ATCCATTGAC AATCCAACGC ATTCTCTACT GACAAAACCG 
CTGGTGAATA CCTCAGATGA CGAATCTCGA TTCCGTCTCA AACGGCCTCG AAACGAGATC 
AAACCAGCCT CCCACAATGA GTCGACGTCG TCCACGGTAT CACGTTTCCA GTTCAAGCGA 
CCAGCGCCAA ACAGCACGCC TTGCAGTCCT AAACCTAGCA ACTCGACGAC ATCGTCAGCA 
GACGATGAAT CCCGGTATCG TTTGAAACGA CCTGCGCCCA AAACTCAAAC CAATGCGTCC 
AACAAACCGA CCACACCGAA CCCGATGCTG GTCAGCCAGC TCAGTTTCGA CATGTCCTTC 
GATACGGAGG CTCTCGCTCT GGAAGAAGCA CCGCAACAGC CCAAGCCGAA GCCATCACTA 
TCCGAGCAAA CCTTGAACAA AATGCGGGTC TTTGAGTTTA ACCGGGGTCC GGATGACGGT 
GCTTCAAATT CCAAAGCTGA TCCGGAAGAG AAGAAGCAGC GTGATGATAG CGCATACGAA 
TCGCAGCCGG CATCGTCGTC GTCCGCGTCC ACATCCGGTT CAGGTAGGTA CTGGAAGCGA 
GACTTGAAGT TGCCGGAACT GAGTCACGTG GCCACTCTCG GTGGATCTGG TGACGTGGAT 
GCTGAACTGG CGCTGCTCGA GTCGGTTGAT TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL008178 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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