LOC5568954 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5568954
Entrez Gene ID	5568954
Full Name	titin
Synonyms	AAEL007258
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021854098.1	XP_021709790.1	titin isoform X1
XM_021854096.1	XP_021709788.1	titin isoform X1
XM_001652567.1	XP_001652617.1	AAEL007258-PA
XM_021854097.1	XP_021709789.1	titin isoform X1
XM_021854099.1	XP_021709791.1	titin isoform X1
XM_021854100.1	XP_021709792.1	titin isoform X1
XM_021854095.1	XP_021709787.1	titin isoform X1
XM_021854101.1	XP_021709793.1	titin isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5568954 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5568954 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe04745	XM_001652567.1 Latest version!	Aedes aegypti AAEL007258-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe129075	XM_021854095.1 Latest version!	Aedes aegypti titin (LOC5568954), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe129075	XM_021854098.1 Latest version!	Aedes aegypti titin (LOC5568954), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe129075	XM_021854096.1 Latest version!	Aedes aegypti titin (LOC5568954), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe129075	XM_021854097.1 Latest version!	Aedes aegypti titin (LOC5568954), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe129075	XM_021854100.1 Latest version!	Aedes aegypti titin (LOC5568954), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe129075	XM_021854099.1 Latest version!	Aedes aegypti titin (LOC5568954), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAe129076	XM_021854101.1 Latest version!	Aedes aegypti titin (LOC5568954), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5568954 ( XM_001652567.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001652617.1 Aedes aegypti AAEL007258-RA partial mRNA.

Price & Availability↑

CloneID	OAe04745
Clone ID Related Accession (Same CDS sequence)	XM_001652567.1
Accession Version	XM_001652567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL007258-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810947). COMPLETENESS: incomplete on the 5' end.

ATGCTAGCCA AAGCAAAAGC GCAACCTCCT GCTCCTAAAC CACCACCTCT AGCAGCACCG 
TCGTCAAGTG CATCAACAGT GACCGAAAAA GAAACTCCGT CAGAAGTGAT AGCCACCGAC 
AGGACTGTAG AAGCAGAATC GGAACCGTTA CGACCCGATG TTGAGGAACC TCCAAAGCAA 
ACATCTTCAA CTTCGTCGGC ACCACCCAAA ACAGGGGCAG GAGGAGGCTC TACACTGTTC 
AGGAGGAAGA AGGTAGTACC AGTGGCCATA GCACGTAAGC TAGCTCAAAA AGAAAAGACT 
AAACCCGCAC CAAAACAGTG TGAAGAAAAA ACTCCCCCGG CGACATCACC TACGGATGCA 
TCACCAGTTC AATGTGAACC TGTTCTACCA GAGCCGGTTG AGCAGGTACC TCAACAACCT 
GAATCTGCTC CTGTAGAACC CAAACCGGTC GAACCTGAAT CTTCTCAACA CGAAGCAAAA 
GAACCAGCAG CGTCTTCAGA ACCCAACCCG GTGGAGCAAA CCGAGACCAG AGAGAAACCA 
GTGGCTAGCC CACCACCTCT CGTTCTGGTG AAACGTGAAC CGTCAGATCT GGTGACACGT 
CCGACGGCAT TCCGCAGAAT CAAACCAAAG GTTGCAATCT GTCTCAATCC AAGCCGTCCG 
AAGAAGTCAA TTCTCAAAAA GACCACTTCT GCGGACAAAT TGCTCCCGGC GGGATCGAAC 
GAGCCGAGCA AATCTTTCAA GGATTTGATT CCAGAGGAGA CGATTGTGCT GGACGATGAT 
GATGAGGAAG ACAACGAGCA GCACACGCCA AGCGTTACGG TTGAAGAGGC TGATCATACA 
GGAAAGTCGG AGGTCCAGCG AACGCAGCAA GAGGAAGCAG AACGTATGGC TGAGGAGGAA 
ACGCGACGAA TAGCGGAGGA GCAACGTCAA AAGGATGCTG AAGATCAGAG AAGGATAGAG 
GAAGGGGCTA GGAGAAAGGC TGAAGAACAG CGTAGGATAT TAGCGGAACA GAAGAAACGA 
GAAGAGGAAG AAAAGCGACG AATAGCGGAA GATCAACGGA GAGCTGAAGA AGAGGCTAAA 
AAAGTGGCTG AAGAACAGTG CAGAATACAA GAAGAAATGA AGAAACGAGA AGAAGAACAG 
CGACAGAAGC TAGAACAACT ACGAATACAA GAAGAACAGC GGAAAGTTGC AGAGGAACAA 
AAGCGGTTGG AAATGCTCGA GCAGGAGGAG AAAGCCAAAC GTGTGGAACC AGAGAAACCC 
GCAGCTGAAA GTAGGAAAAC CATGAAAATA CCGCCGGAAA TTTGGAAACG ACAAATGGAG 
AGGATGAAAA ACAACCCGAA CCGACAGCAT AGTACGGTGC CGGCAATTCC ACAGTCGAAT 
GCGGAACCAT CGCAATCTCA ACACACGATC ACATCGGATC CTGTTAATGT TAAAACATCC 
AATGTGGTCG AGCAGGAGCC TGTTAGTGTT GTAGCTCCTG CTGTAAATCA AGAAAGAGCT 
AACCAAATCA GTGAGAAGCC TGCTGTGCAG GTGACTCCAA ATCCTACTAA AGAAATCCGA 
CGAATATCTC GGCTTCCTCC CAGTTTGGCA ATGAAGCTGA TGAACTGTGT CGCTTTGAAG 
CCTTCCAGGA ACAAAATAGA GGAGAAGGCT GCACCGTCTG TAAAACCCTC ACAGAATAAA 
ACAGAAACAT CGCATGCAAC GAAAGAAGTA CCATCATCGG CACCAGCAGT AGTTTCACAA 
ACTGCAATTA GGCGAATATC CGGGCTTTCA CCGAGTGCGA GTATGACGCC AATAAAAACT 
GCTGAAGCGA TGCAACAGGA AGTCCAGGAA ACGACAGCCG ATAAACAGCA GCAGTCAATT 
GTACCTGAGA GCGCGGTCGC AAATGTTGAG AGAACCGAAA AGACTGAACC GGTGGAGCGT 
ACTGTGATAT CTAACAAGCC GGCGCCGACG AAAGAGATTA AACGGATAGC ACTTCCGCCA 
GATATTGCTT TGAAGTTGTT AGGAGCAGTT AGTAGAAATA AACCGGTGGT TGTGACATCC 
ACGGCCGGAA CAGTTGATCC AGCGAAAAAT GTTGCATCTA AACCTGCAAC GATCGCTGAA 
AAGCCTGCGG TCGCTGAACC CGAGTCGTCG AACCCTACTG TAACGGCCGC TGCTCCCATC 
GTTGTTCCTC CAATAAAGCA AACAACAATT CAGAGTGGTA CAAATGCTCC GAAAGAAATC 
CGTCGAATAT CACAACTGCC TCCCAATGTC GCGATGAGGC TGGTGGATTC TTTGAAACAG 
AAAGGAATAA CTGTACCAAA GTTGTGTCTT AAGAAGGATA CTCCTACCAT CGAACCAGTT 
AGTGTAGCTG CATCAGATGT ACAACTTCCC AGTGAACCAT CGACACCGTC TTCCGTGGCA 
TCGGTGCCGG AAATTAAGGT GGAACCACAG GAGCAGCCAA CCAAATCCAA AGGAAAGGTG 
GAAATCTTGT CACAGGTTAT TTTGCCTACG GCATCCTACA ATCTGGAGGA GCTATTGGCT 
GGCAAATCAA CTGAAGGCTT TGCGCCATCC TGTGCGATGC CTCTTCCGTT ACCCATGCAC 
AAAGGAAGAA GGCAAGCGGC TCCTGTCCCT ATTGCGCCAG TTCCTACATC AATTCATAGT 
GAGCCGATAC AGCATAGTCA AGTCTCTTTA CCTGTACAAC AGCCTAGCAA ACCAGAGACT 
CCTGCACCGG TTACTGCGGT ACCATCGCCG ATTCCAGATC TTCCGGTTGC AATGGCAGAA 
AATGATATCG CTTCTGAAGT CACTGTCACA ACGGAGAATG AAGATATCCC AAATCCATCG 
CATGAAAAAT TCGACGAATA CATTATTTCC TATTCAGATT GTGAGGGAGC TCCAGTGCAA 
TATGATCCAT CAAAAAGCGC CCAACAGCAA AATAAGGAAG AAATCCCACC TGAGCCGAAT 
GTTACTTTTG TTTCCTCAAA CGCTCCTACT GTTATGTCGC ATCAATATTC CGTTACGTAC 
TCAGCGAATT CACACCAGCA ACTTCATCGG CCAAACGTTC AAGCTCCGAT AGTAATTCAA 
CAACCAGCAA ACGTTTCCAG TTATCAGCCA CGCCCCGTCG CCTATTTCAC CAAGGTATCC 
AACAATACAC AACGACCTTC CCCTCAACCA ACACCCGTAA CATATCATCC ACAGTTTACG 
GTGTTATCAC AGCCCGGAAT GAACCCTACC CGCATGATAT CCCAGCGAAT GATCATCAAT 
CCAGCAGCCA GCATGCAAAG TGGCCAAAGA CCTCGGTATC CAACAGCTCA GTTTGTTGTG 
CTACGCCACC CCACTCCAAT CAGAGGACAG TTAGTTGCTC AAACTCAACT TCGGCAGGAA 
CATCCACAGC CTGTGCCGCA ACAATCGCAA CAGCATCTAA CTGTCCCTAG AGAAACCTAT 
TATCATCGCT TGCCAATAGT CAACCAGAAT CAGCCTTCAG TTATTGTACC AACGCCGCGA 
TACGTTATCC CCAAACAGTA CCCTCAAATT CAAATGGCTC GTCCTCCGCG TCCGCAGCGT 
CCTTCCGTAG ACATCGCCCA AAATCAAAGC AAAGCATTCC AACCTGTTCA ACAATCGCAA 
CCGACCACCG CATTCTCCAT CGCTCAACCT GTACAACAGC AACGTGTAAT TCCGAAGATC 
GTCATCGACA GCAGCTACGT GGATCAGCAA GAACGAGGGA CTCCAGCTTC TTCAAACACT 
CCACCTCTTC AAGATCAATG GAAAACACAG AATACGGCAC CCTGTTCGGA AGAGATCAGG 
AACAAACTCC GGGACAAATT GAAGTCTCGA ATGCTGCAAC AAAGTGCCAA ATCAGGAAGC 
CCAACTGTGG AAACCCCACC GCCAAACCCA CTATCAACGC CTTCATCAGC GGCTCCCAAA 
GCCTCACCAG CAAAAACTCC GGTTGGACCT CCTACAATAC CCGTTCGTAA CACCGTAGTT 
TACCATCCGC AAGACTTCCA GCAACGCATT CGTTCACAAC AAGCGCAACC ACCCAAACCG 
GTAGATACTC GCATCATCAA GCAGTACATA TGTCAGGGAA GGAAAATTTA CCTCACCAAA 
ATCAGTGTCA CAAAACCCAC ACAGGCCAGT TCCTCGAACG TTCAGCAGAC AGAGCCACCT 
CAGCAGTTCC TACAGTATCG GCAGCCCCAA ACGGACACGG GCTTTCATGG TTTCCCACAG 
GCAGCGATAA AATGCGATCA ACGCAATTTC CAGCAGTTCA ACGAAACGGT TCAGCAGTTG 
GAGCGGAGGA AAGTTCCAGA ACCTTCGGAT GAAAGCGGGA TGATTGAAAT TCAGAATATC 
CAGAAAATTC GTGCCAAGAT GCGAAATCGG AAACCGAGTG TGGTGACGGC TGTAACAACA 
CACGGCTTGA AAGGAGAAAC TTTCATCATT ACGGAACTGA ACGATCCGTC GCTGCATCCA 
ATGGAGATGC ATTCACCGGA AGTGAAGCAA GAAGTTCCGC TACAACATCC TCCTAGAATC 
ACCAGACGAA GAAATACCTT TGTAGTGGAT GATTCGGATG GAGACGTTCA AACCGATCAG 
TTACCACAGG TGGAAATGAC TAGTCAGGAT GATAGCCAAC AGGATGATAA ACCATCTGAA 
ATGGAACTCA AGGTGGAAGT CGAGAATGAG GAAAAGGAAC CGGTAACGAA CGCGGAACGA 
TTAGCATTCG ATCGGTGTAT GAAGGAAGAG GTACCATCTG GATCCGAATC CAGCCAGGCT 
GACGAAGATC TGGCACGGGA GATTTTGGCT CTGGTGGAAA ACGAACGCAA GGCTTTGCGC 
GCTGGGCCAA CGGTGAAGAA AGTCAAGAAG CGAAGAAAGC GACGTAAAAG GCACAAGAAG 
AAAAGTGATG GCAATGAGGA GTTGATGGAT CTCTTGATGA AGCATGTCAA TCAGGAGTCG 
TTAATTGCGG ATATCGTGGC CTCGCAGCAG AAGAAATTGC CTGTCATTGA GCTATCGGAT 
ACGGATGAGA ATGATGAGGA TAGAACAGTG CAGAGGAGAG TGACCTGCAA GGATGTTCGA 
GTGAAGCCAG ATCCTGATGC GGAAACAATC GGCGATAGGA AAACATCGTG TACGAGTGAC 
TTCGAGGAAT CTGAGACGGA TGACATTTTT GCCGAGATTT TGAAACCGTT TGTCGAGGAA 
GAGGAACATG CGGTTGGATC GGAAAGAGTT GTGGTGGAAG AAACGCCGCA GAAAGTTGTC 
TCAGGTGAAC CGATGGCTGA GTCGGATAAA CCATGTGCAA AGGAAATAAC TGGTTCAGAT 
GAGATTCTAA GTACGCTTGT CGAAAAAGAG AAACAAGCAG TTAGCCTTGA AGGCGTAGCG 
ATTCAAGAAT CGTTGCAGAA TGCTGTTCCA GGGGAATCAG AGGATGCATG TCCAGAGCAG 
CCGTCAGCAC CAGCGCAGAA AGCTACTTCA GAAGACATTT TGAAATCGAT TGTCGAGGAA 
GTGAAACAGT CGAATGAAGC GAAAGCTGTT CCTGAGAAAC CGGCACAAAA ATCGGATGAA 
GCATGTCGAG AAGCGCCGAG AGTACTGGCG GATGAAGTTA TTTCAATACC TGCAGTGGAA 
GAAATCGTTC CAGAGAATCA GGTAACATCA GAGGAACAGG ATGTTGTTCC AGAACAGCAA 
GCAGTAACAT TGAATAAGGT CGTTACCGAT GAGCAGAGAG AAACGATACC AGAAGAAAAG 
GCACTTTTAC CAGTGGAAGC CGCTCCGGCG GAACAAATAG TGCATGTGCC AAAAGAAGCC 
GAAACATCTG AGATTTCATC AAAATTAGTT ACGGAAGTCA GACCAGAGAT TGAATCGAAT 
CAACCTACCA TATGTGATCA AACCTGTGAT AACAGTGCCT TACCGGTCAA AGAGCTGCCG 
AAAAAAGCAC CTGCTCAAAG AGCACCCAAA CGCAAGCTCA AACCAACGTT AGTTTCTCGA 
TCAAAGATAA GAAAAGTTGA ACCGCCAGCA GCGATAGTCA CCAAAATCCA ACCACAACCA 
ACAAGAGTCG AAGAGTCAAC GGAAGTCAAC ATTGAAGATT CCAGTGATCC GTTCTCAGAT 
CGAGATGAGC CGCCACTACC GGATTCGGAG GAAGAGCGAA TCCAGTCTCA AATGGATACG 
ATCAACTCCA GAGTACGGGA ACTAGTCAAT GAGTGGGATT CTGATGAAGA AGAGACAAGT 
ATAAAGCAAC CATTGCAGAA ATATGCCTCG TTCGATAACA ACGGTAATCT TCCGTGTCGC 
ATTTCAAAAA ATAATGCGTT GAAACGTGCT GCGATACTGA GCGATCCGGT TTTGGAAGTT 
TCTGTTGAGG AAAGCCGCAA GGAGGAATCA CACGAAGCGA TTGAGGATAC TTCGGTGCAA 
GCTACAGCCA ACATCGTTAA GCCGGTAAGG GTTACCGTCC TAAAGGTGGA TTTGATAGAA 
CGATCAGAGA TCGTACAAGA GCCGGTAGAA CCCGCTACAA GAGAGAAGAC TCCGCCGCCA 
CCAACTGAAG CGAAGGGTCA AACCGTCGCG AATTTGTTGA AGGACTGGGA TGAATCACCG 
ATCAAACAAA AAAAGGAGAA CGCTCCAGTG GTTGCTCGGG TGCTATTAGA TAGACTCAGC 
TCATCTGAGT TAAATCAGTT GAAATCCAAA AAGGCTCCGG AAGTGTCAGA ATCAAGTAAG 
ATCACGATGG AACCATCGAA TCAACCACCA ACGATTGAAA GTAAGGCGAA GGATAGTCGA 
GATGAAATAA GCGGTGACGA ATCGGAGAGA GTCGTCGCTC GTATATTTAT GGGCGGACTA 
GACAGCTCTG GATCATCTGG ACAGCAACAA ATAGTGCCAC CAGCATCACA GATGGCGGCT 
CAAAGCTCTC CAGTTTCTAC TGGAAGTCCA AGCAAATCTC GTAAAAGTGA AAACAAGATT 
CCAAATACGG CCGAATCTCC TACCACTCGT TCGAAGTCTT CCAAGCAGAC ATCTATCAAA 
GATTGGATTC AAGGTTCTCC AACTACTGAG CCTTCGAAGC CTATTACGGG TAGGAGGAAA 
AGCTTGCGTG GAAGAGCCGC ATCTGTTCTA TTTGAACCAG AGGCTAACCA AGTCACTGAA 
AAACAGAAAC CTGCAGGCAG GAATCTGAGA AGCCGTACTA AATCCGTTTT TGCCGAAAAG 
CCACCTCTGA TGGAAGAAGA AGAACCGGTA CAGCAAGCAG ATATATCACG ACCAGTGGAG 
GAACCGTCTG AGAAACAGAT TGAAGTAACA CCGGAAAAGC CCAGCGGAAG TCGTCCGGAG 
GTGATAAAGG AAGCAGGGGG AACCGCTTGT GCGGCACGGA AAGAACAAGA ATCGAAAGAA 
TGCTCTACTA GCACTAAACC TACGCCGATC ATTCGGAACC GTGTTCCGAT TGCGATATCC 
TCGGAAGACG AAGATGATGC CCCGTTAGCC AAACGTCGCA AAGTGCTTCG CAAGAGGCGT 
TCCATGATGC GCCGTCCAAG CGCAATCCGG GATCTGAATG CTAACAAACG TCTGTTCCGA 
GGAGGCGCAA CTCTCAACAA TTCTCTCACC GTAGATGATT CCAACATGGG CGAAACTACC 
TTCATCAAAA GCGAACCTCC GGAGGAGATA GAAGATATTC AAGATTACTA TGCCTCTCCG 
AGTGAATCCT TTGCTGTCAC ATTCCCGGAT AATCCCTCCG AGCAACTGCT TTCCGTGGTC 
ACTGTGCAAA ACAATCAAGT AGTGCACTCA CAAGACTTCG ACGCTGGAAG TCCCTTCCGG 
AACCAGTATT TTGCCAAAGA TCTTATTCAA TCGGCAGCGA CTTCCACTCC TAACGCCCAA 
TCGCCGATCC AGCCTCCTCC TCCCGTACAG GATCATGTCT TTGCCACTCC GGAACCCGTC 
CGTGCGGCAA AACGGAAGCG TCGTACGAAA CTGGAAATGG AAAACGATCG AAGACTTGAA 
GCGATGAAAA GGGAAGAAGA GCAGAAGCCT GAAGAGCTCA AGAAAAAGGA AGCACAACGA 
GGCAGAGTGT TTAAATCCTT TACGCCAGTC GAGGTGAATG CCGTTGATCC GAATATTAGC 
CAGTTTGAAT GCGCTTCGTG TTTCGAGCAC GTTTCGCGTG ATGATTGGGA TGCTCACTAT 
GCGAAGCACA GTGGTCTTAC CTACCGAGTG AACGTAGATC CTGTGGTTGA CCTGAACGAC 
GAAACAACGG CCGTGGCAAT TGTGACACGT TTCATGAAGG CCCACCGGCA GGTGACGCTC 
TACTGTGACA AGTGCAATGT GGCCAAGAAA TCGGCCCTCG GATTAGTGTC GCATCGGCCT 
GTTTGTGGTC TGACGCGGCA AGAGATCCAA AACACCAAAA TACCGTGCCA GCATTGTGGC 
CGGAAGTTGA TGCCCGTCAG TATGAACTGT CACCTGTTGG GACATTGCGC GGTGCTGAAG 
CGAAAGAAAC AAGAAGAAGC TTTGGAGACG AAGCTGGAAG GAGCGTCGGC CGATGCGGAA 
ATGGAACCAA CAACGCTCAA TCAGCGTGGA ACGGTTAAGC GCCAAGCGAC CGCCAAGGCC 
GAGAGGCGCA TCCAGAACAT ATCGTTCAGC GAAATGATTC TCTCCGTCAC GAAGGACAAG 
GTTACCGAGG GTTGTTTGGC TGCTTGGAAA ACACAGTTCC GCAAGGCTAA GGTGGCGCTG 
TGCGTTTTTC GGACGTGTTC TTTCACTGGG ACCGACGAAA TAGAAATGCG CCACCATCAC 
GAGTTCTGTC CTTCGCCCTG TGAGCTACTC GAGTGCAAGA ATTGCAAGTT TCAAACGGAA 
AAGATCGACC AAATGGTGAA ACATATCAAG CTGAAACACA ACGACGTGCT GGTTTGTATG 
ACGATCGATG ATAGCAGCGG AACGGATGCC AAGATGACGT CGGAATCATC GGACGACGAG 
GGCGGTGCGG GTGTAGACGA AAACGATGAA CCATACATGA TTGGCATTGA CGATGAGGAC 
AAGCCGAAGA GGCGAAAAAT CAAAACTCTG CTGAAAACGT GCAAATTATC TACCAGGGAT 
ACGTTCAACG AGGAAAGCGA CGTTTATCGA GAGATGATCC TAGACGAAGT GATCAATCTT 
CGGCTGCTCA AGAGTGAGTT CCATCTGACG GCACGCGAAT GGACAGCGGA GTTCCGACGG 
TTGCATTATT CGACCAGCAT CCTGTTTTCA GCGTTACGAC CGGATTTGGA CGTACGGTTT 
CTTACGTTTA ACATGACCCA GGAGTACATC CCGAAGCAGA CACATTCTTT GCGGTTTGTC 
GCTCGGAGTA CGAATCTGTA CAACGCTCCC ATTCAACTGA AAGAATACTC CAACAAGTGG 
CAGAACTTGG AGGTTTTTAC CGGGCAGGTG AATGAGAAGG ACACGATATT CTTCTGTGGG 
GGACCCGTTG TTGCTCTAGA TTGGCTGCCA ATACCGGACG GCCAGCATGA ACATCAGATT 
GTGGCGGTGG CTTGCAAGAA CGATTTCGAA GAATTCTACC TGGCGGATCG CATCTTCCCG 
GTGAAGTGTT TGATTCAGAT CTGGGACGTG GGCTTACTGG ACAACAAAGA AATCTACAAC 
AAAAAAGCGG AGAATGCCAA ACCCACCTTA CTGTACTCCA TAGCGTGCGA CTTTGGTCCG 
ATTTGGGCGT TGAAGTTCTG CCCGAGTGGA TGTTACAACT CCAAGACCTC CGGCGACGAT 
TACGATCGGT TGGGCTTGCT TGCCGCGGCC GGGTCGGACG GAAATATTCA CATCTTTTCG 
CTGGGGCGCA GCTATGAACA CCTGATCTCG AACAGTAGCC GAGTGATCAA CTTGGCTCCG 
GTGCTCAAGC TGACTCTGTC CTTAGCGGAC GAAAGCTGTT GTTCGTCGTA TGAGTCCCAC 
AGTGCCGTGA AGTTGGCGTG GTCCAAGAGC AAACACCACT CGATGTTGGC AGCGGGCTAT 
TCGAACGGAA CGGTCGCAGT GTGGAATGCC ACGTCAACTT CGCCTCTGTT GACCGGAACG 
AAAGATAACG TTCGGGCACT GCTTCCCGTT CATAAGGTGT TCATTCCCGA TTCGTGCGTG 
ACCGCTGTCG ATCTTCATTA TTCGGAAGAT TCTCGTTATC TTTTGGTATG TAATGCGGAC 
AGGAAGGTCG TTGTCTACGA CCTGAACACG GGCTACATGC CGATGGAGAT TTGTAGTTTG 
AACGCCCGTA GTAAAGTTAC TACGGCTTGT TGGAATATCC ACTTCCCGCT GATAACGATG 
GCTTTCGACG ATGTGTATGC CATCGACCGG TGCGCTTTGA CGTTCCATCA GCCCCGTGAG 
ATTGGCGTGC GGTTGCATCC GTTGTATACT CATACGGCGG AAGCAACTGA CATGAGCAAC 
AGCGATCATC TGTCGACGCA CGTGATCGGT ACCGATGGCG GCGACGTGCT GAGCCACAAA 
CCGATGGCCT TTATTCACAA CATGGGCCAG AAGAATCCCG CGCAGATTAA ATATGTTTTG 
ACGTCAACAA TATCGGTGAA AGTCAATGAA GAACAAATGG ATAACTCTTA CAACAAGTTC 
GAGAAGAACT ATTGCGTGCT GTTTTCGGAC TTCGATAAGA ACCCGGCTCG AATGGACCTC 
AAATCGCTAC AGGTGAAAAC GTTCCGACGT GCCCTGCTGC ACGAATACCC GGGCATCCGG 
ATTAACCAGG TCAAGTGGAA TCCCAACGAC AAGTCCCATC AATACTACGC CATCGGGTAC 
CAGGCTGGCT TTGTGCGTGT GCGACCCTTC CGGCTGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001652617.1
CDS	1..10722
Translation	MLAKAKAQPPAPKPPPLAAPSSSASTVTEKETPSEVIATDRTVEAESEPLRPDVEEPPKQTSSTSSAPPKTGAGGGSTLFRRKKVVPVAIARKLAQKEKTKPAPKQCEEKTPPATSPTDASPVQCEPVLPEPVEQVPQQPESAPVEPKPVEPESSQHEAKEPAASSEPNPVEQTETREKPVASPPPLVLVKREPSDLVTRPTAFRRIKPKVAICLNPSRPKKSILKKTTSADKLLPAGSNEPSKSFKDLIPEETIVLDDDDEEDNEQHTPSVTVEEADHTGKSEVQRTQQEEAERMAEEETRRIAEEQRQKDAEDQRRIEEGARRKAEEQRRILAEQKKREEEEKRRIAEDQRRAEEEAKKVAEEQCRIQEEMKKREEEQRQKLEQLRIQEEQRKVAEEQKRLEMLEQEEKAKRVEPEKPAAESRKTMKIPPEIWKRQMERMKNNPNRQHSTVPAIPQSNAEPSQSQHTITSDPVNVKTSNVVEQEPVSVVAPAVNQERANQISEKPAVQVTPNPTKEIRRISRLPPSLAMKLMNCVALKPSRNKIEEKAAPSVKPSQNKTETSHATKEVPSSAPAVVSQTAIRRISGLSPSASMTPIKTAEAMQQEVQETTADKQQQSIVPESAVANVERTEKTEPVERTVISNKPAPTKEIKRIALPPDIALKLLGAVSRNKPVVVTSTAGTVDPAKNVASKPATIAEKPAVAEPESSNPTVTAAAPIVVPPIKQTTIQSGTNAPKEIRRISQLPPNVAMRLVDSLKQKGITVPKLCLKKDTPTIEPVSVAASDVQLPSEPSTPSSVASVPEIKVEPQEQPTKSKGKVEILSQVILPTASYNLEELLAGKSTEGFAPSCAMPLPLPMHKGRRQAAPVPIAPVPTSIHSEPIQHSQVSLPVQQPSKPETPAPVTAVPSPIPDLPVAMAENDIASEVTVTTENEDIPNPSHEKFDEYIISYSDCEGAPVQYDPSKSAQQQNKEEIPPEPNVTFVSSNAPTVMSHQYSVTYSANSHQQLHRPNVQAPIVIQQPANVSSYQPRPVAYFTKVSNNTQRPSPQPTPVTYHPQFTVLSQPGMNPTRMISQRMIINPAASMQSGQRPRYPTAQFVVLRHPTPIRGQLVAQTQLRQEHPQPVPQQSQQHLTVPRETYYHRLPIVNQNQPSVIVPTPRYVIPKQYPQIQMARPPRPQRPSVDIAQNQSKAFQPVQQSQPTTAFSIAQPVQQQRVIPKIVIDSSYVDQQERGTPASSNTPPLQDQWKTQNTAPCSEEIRNKLRDKLKSRMLQQSAKSGSPTVETPPPNPLSTPSSAAPKASPAKTPVGPPTIPVRNTVVYHPQDFQQRIRSQQAQPPKPVDTRIIKQYICQGRKIYLTKISVTKPTQASSSNVQQTEPPQQFLQYRQPQTDTGFHGFPQAAIKCDQRNFQQFNETVQQLERRKVPEPSDESGMIEIQNIQKIRAKMRNRKPSVVTAVTTHGLKGETFIITELNDPSLHPMEMHSPEVKQEVPLQHPPRITRRRNTFVVDDSDGDVQTDQLPQVEMTSQDDSQQDDKPSEMELKVEVENEEKEPVTNAERLAFDRCMKEEVPSGSESSQADEDLAREILALVENERKALRAGPTVKKVKKRRKRRKRHKKKSDGNEELMDLLMKHVNQESLIADIVASQQKKLPVIELSDTDENDEDRTVQRRVTCKDVRVKPDPDAETIGDRKTSCTSDFEESETDDIFAEILKPFVEEEEHAVGSERVVVEETPQKVVSGEPMAESDKPCAKEITGSDEILSTLVEKEKQAVSLEGVAIQESLQNAVPGESEDACPEQPSAPAQKATSEDILKSIVEEVKQSNEAKAVPEKPAQKSDEACREAPRVLADEVISIPAVEEIVPENQVTSEEQDVVPEQQAVTLNKVVTDEQRETIPEEKALLPVEAAPAEQIVHVPKEAETSEISSKLVTEVRPEIESNQPTICDQTCDNSALPVKELPKKAPAQRAPKRKLKPTLVSRSKIRKVEPPAAIVTKIQPQPTRVEESTEVNIEDSSDPFSDRDEPPLPDSEEERIQSQMDTINSRVRELVNEWDSDEEETSIKQPLQKYASFDNNGNLPCRISKNNALKRAAILSDPVLEVSVEESRKEESHEAIEDTSVQATANIVKPVRVTVLKVDLIERSEIVQEPVEPATREKTPPPPTEAKGQTVANLLKDWDESPIKQKKENAPVVARVLLDRLSSSELNQLKSKKAPEVSESSKITMEPSNQPPTIESKAKDSRDEISGDESERVVARIFMGGLDSSGSSGQQQIVPPASQMAAQSSPVSTGSPSKSRKSENKIPNTAESPTTRSKSSKQTSIKDWIQGSPTTEPSKPITGRRKSLRGRAASVLFEPEANQVTEKQKPAGRNLRSRTKSVFAEKPPLMEEEEPVQQADISRPVEEPSEKQIEVTPEKPSGSRPEVIKEAGGTACAARKEQESKECSTSTKPTPIIRNRVPIAISSEDEDDAPLAKRRKVLRKRRSMMRRPSAIRDLNANKRLFRGGATLNNSLTVDDSNMGETTFIKSEPPEEIEDIQDYYASPSESFAVTFPDNPSEQLLSVVTVQNNQVVHSQDFDAGSPFRNQYFAKDLIQSAATSTPNAQSPIQPPPPVQDHVFATPEPVRAAKRKRRTKLEMENDRRLEAMKREEEQKPEELKKKEAQRGRVFKSFTPVEVNAVDPNISQFECASCFEHVSRDDWDAHYAKHSGLTYRVNVDPVVDLNDETTAVAIVTRFMKAHRQVTLYCDKCNVAKKSALGLVSHRPVCGLTRQEIQNTKIPCQHCGRKLMPVSMNCHLLGHCAVLKRKKQEEALETKLEGASADAEMEPTTLNQRGTVKRQATAKAERRIQNISFSEMILSVTKDKVTEGCLAAWKTQFRKAKVALCVFRTCSFTGTDEIEMRHHHEFCPSPCELLECKNCKFQTEKIDQMVKHIKLKHNDVLVCMTIDDSSGTDAKMTSESSDDEGGAGVDENDEPYMIGIDDEDKPKRRKIKTLLKTCKLSTRDTFNEESDVYREMILDEVINLRLLKSEFHLTAREWTAEFRRLHYSTSILFSALRPDLDVRFLTFNMTQEYIPKQTHSLRFVARSTNLYNAPIQLKEYSNKWQNLEVFTGQVNEKDTIFFCGGPVVALDWLPIPDGQHEHQIVAVACKNDFEEFYLADRIFPVKCLIQIWDVGLLDNKEIYNKKAENAKPTLLYSIACDFGPIWALKFCPSGCYNSKTSGDDYDRLGLLAAAGSDGNIHIFSLGRSYEHLISNSSRVINLAPVLKLTLSLADESCCSSYESHSAVKLAWSKSKHHSMLAAGYSNGTVAVWNATSTSPLLTGTKDNVRALLPVHKVFIPDSCVTAVDLHYSEDSRYLLVCNADRKVVVYDLNTGYMPMEICSLNARSKVTTACWNIHFPLITMAFDDVYAIDRCALTFHQPREIGVRLHPLYTHTAEATDMSNSDHLSTHVIGTDGGDVLSHKPMAFIHNMGQKNPAQIKYVLTSTISVKVNEEQMDNSYNKFEKNYCVLFSDFDKNPARMDLKSLQVKTFRRALLHEYPGIRINQVKWNPNDKSHQYYAIGYQAGFVRVRPFRLK

Target ORF information:

RefSeq Version	XM_001652567.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL007258-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001652567.1

ORF Insert Sequence:

ATGCTAGCCA AAGCAAAAGC GCAACCTCCT GCTCCTAAAC CACCACCTCT AGCAGCACCG 
TCGTCAAGTG CATCAACAGT GACCGAAAAA GAAACTCCGT CAGAAGTGAT AGCCACCGAC 
AGGACTGTAG AAGCAGAATC GGAACCGTTA CGACCCGATG TTGAGGAACC TCCAAAGCAA 
ACATCTTCAA CTTCGTCGGC ACCACCCAAA ACAGGGGCAG GAGGAGGCTC TACACTGTTC 
AGGAGGAAGA AGGTAGTACC AGTGGCCATA GCACGTAAGC TAGCTCAAAA AGAAAAGACT 
AAACCCGCAC CAAAACAGTG TGAAGAAAAA ACTCCCCCGG CGACATCACC TACGGATGCA 
TCACCAGTTC AATGTGAACC TGTTCTACCA GAGCCGGTTG AGCAGGTACC TCAACAACCT 
GAATCTGCTC CTGTAGAACC CAAACCGGTC GAACCTGAAT CTTCTCAACA CGAAGCAAAA 
GAACCAGCAG CGTCTTCAGA ACCCAACCCG GTGGAGCAAA CCGAGACCAG AGAGAAACCA 
GTGGCTAGCC CACCACCTCT CGTTCTGGTG AAACGTGAAC CGTCAGATCT GGTGACACGT 
CCGACGGCAT TCCGCAGAAT CAAACCAAAG GTTGCAATCT GTCTCAATCC AAGCCGTCCG 
AAGAAGTCAA TTCTCAAAAA GACCACTTCT GCGGACAAAT TGCTCCCGGC GGGATCGAAC 
GAGCCGAGCA AATCTTTCAA GGATTTGATT CCAGAGGAGA CGATTGTGCT GGACGATGAT 
GATGAGGAAG ACAACGAGCA GCACACGCCA AGCGTTACGG TTGAAGAGGC TGATCATACA 
GGAAAGTCGG AGGTCCAGCG AACGCAGCAA GAGGAAGCAG AACGTATGGC TGAGGAGGAA 
ACGCGACGAA TAGCGGAGGA GCAACGTCAA AAGGATGCTG AAGATCAGAG AAGGATAGAG 
GAAGGGGCTA GGAGAAAGGC TGAAGAACAG CGTAGGATAT TAGCGGAACA GAAGAAACGA 
GAAGAGGAAG AAAAGCGACG AATAGCGGAA GATCAACGGA GAGCTGAAGA AGAGGCTAAA 
AAAGTGGCTG AAGAACAGTG CAGAATACAA GAAGAAATGA AGAAACGAGA AGAAGAACAG 
CGACAGAAGC TAGAACAACT ACGAATACAA GAAGAACAGC GGAAAGTTGC AGAGGAACAA 
AAGCGGTTGG AAATGCTCGA GCAGGAGGAG AAAGCCAAAC GTGTGGAACC AGAGAAACCC 
GCAGCTGAAA GTAGGAAAAC CATGAAAATA CCGCCGGAAA TTTGGAAACG ACAAATGGAG 
AGGATGAAAA ACAACCCGAA CCGACAGCAT AGTACGGTGC CGGCAATTCC ACAGTCGAAT 
GCGGAACCAT CGCAATCTCA ACACACGATC ACATCGGATC CTGTTAATGT TAAAACATCC 
AATGTGGTCG AGCAGGAGCC TGTTAGTGTT GTAGCTCCTG CTGTAAATCA AGAAAGAGCT 
AACCAAATCA GTGAGAAGCC TGCTGTGCAG GTGACTCCAA ATCCTACTAA AGAAATCCGA 
CGAATATCTC GGCTTCCTCC CAGTTTGGCA ATGAAGCTGA TGAACTGTGT CGCTTTGAAG 
CCTTCCAGGA ACAAAATAGA GGAGAAGGCT GCACCGTCTG TAAAACCCTC ACAGAATAAA 
ACAGAAACAT CGCATGCAAC GAAAGAAGTA CCATCATCGG CACCAGCAGT AGTTTCACAA 
ACTGCAATTA GGCGAATATC CGGGCTTTCA CCGAGTGCGA GTATGACGCC AATAAAAACT 
GCTGAAGCGA TGCAACAGGA AGTCCAGGAA ACGACAGCCG ATAAACAGCA GCAGTCAATT 
GTACCTGAGA GCGCGGTCGC AAATGTTGAG AGAACCGAAA AGACTGAACC GGTGGAGCGT 
ACTGTGATAT CTAACAAGCC GGCGCCGACG AAAGAGATTA AACGGATAGC ACTTCCGCCA 
GATATTGCTT TGAAGTTGTT AGGAGCAGTT AGTAGAAATA AACCGGTGGT TGTGACATCC 
ACGGCCGGAA CAGTTGATCC AGCGAAAAAT GTTGCATCTA AACCTGCAAC GATCGCTGAA 
AAGCCTGCGG TCGCTGAACC CGAGTCGTCG AACCCTACTG TAACGGCCGC TGCTCCCATC 
GTTGTTCCTC CAATAAAGCA AACAACAATT CAGAGTGGTA CAAATGCTCC GAAAGAAATC 
CGTCGAATAT CACAACTGCC TCCCAATGTC GCGATGAGGC TGGTGGATTC TTTGAAACAG 
AAAGGAATAA CTGTACCAAA GTTGTGTCTT AAGAAGGATA CTCCTACCAT CGAACCAGTT 
AGTGTAGCTG CATCAGATGT ACAACTTCCC AGTGAACCAT CGACACCGTC TTCCGTGGCA 
TCGGTGCCGG AAATTAAGGT GGAACCACAG GAGCAGCCAA CCAAATCCAA AGGAAAGGTG 
GAAATCTTGT CACAGGTTAT TTTGCCTACG GCATCCTACA ATCTGGAGGA GCTATTGGCT 
GGCAAATCAA CTGAAGGCTT TGCGCCATCC TGTGCGATGC CTCTTCCGTT ACCCATGCAC 
AAAGGAAGAA GGCAAGCGGC TCCTGTCCCT ATTGCGCCAG TTCCTACATC AATTCATAGT 
GAGCCGATAC AGCATAGTCA AGTCTCTTTA CCTGTACAAC AGCCTAGCAA ACCAGAGACT 
CCTGCACCGG TTACTGCGGT ACCATCGCCG ATTCCAGATC TTCCGGTTGC AATGGCAGAA 
AATGATATCG CTTCTGAAGT CACTGTCACA ACGGAGAATG AAGATATCCC AAATCCATCG 
CATGAAAAAT TCGACGAATA CATTATTTCC TATTCAGATT GTGAGGGAGC TCCAGTGCAA 
TATGATCCAT CAAAAAGCGC CCAACAGCAA AATAAGGAAG AAATCCCACC TGAGCCGAAT 
GTTACTTTTG TTTCCTCAAA CGCTCCTACT GTTATGTCGC ATCAATATTC CGTTACGTAC 
TCAGCGAATT CACACCAGCA ACTTCATCGG CCAAACGTTC AAGCTCCGAT AGTAATTCAA 
CAACCAGCAA ACGTTTCCAG TTATCAGCCA CGCCCCGTCG CCTATTTCAC CAAGGTATCC 
AACAATACAC AACGACCTTC CCCTCAACCA ACACCCGTAA CATATCATCC ACAGTTTACG 
GTGTTATCAC AGCCCGGAAT GAACCCTACC CGCATGATAT CCCAGCGAAT GATCATCAAT 
CCAGCAGCCA GCATGCAAAG TGGCCAAAGA CCTCGGTATC CAACAGCTCA GTTTGTTGTG 
CTACGCCACC CCACTCCAAT CAGAGGACAG TTAGTTGCTC AAACTCAACT TCGGCAGGAA 
CATCCACAGC CTGTGCCGCA ACAATCGCAA CAGCATCTAA CTGTCCCTAG AGAAACCTAT 
TATCATCGCT TGCCAATAGT CAACCAGAAT CAGCCTTCAG TTATTGTACC AACGCCGCGA 
TACGTTATCC CCAAACAGTA CCCTCAAATT CAAATGGCTC GTCCTCCGCG TCCGCAGCGT 
CCTTCCGTAG ACATCGCCCA AAATCAAAGC AAAGCATTCC AACCTGTTCA ACAATCGCAA 
CCGACCACCG CATTCTCCAT CGCTCAACCT GTACAACAGC AACGTGTAAT TCCGAAGATC 
GTCATCGACA GCAGCTACGT GGATCAGCAA GAACGAGGGA CTCCAGCTTC TTCAAACACT 
CCACCTCTTC AAGATCAATG GAAAACACAG AATACGGCAC CCTGTTCGGA AGAGATCAGG 
AACAAACTCC GGGACAAATT GAAGTCTCGA ATGCTGCAAC AAAGTGCCAA ATCAGGAAGC 
CCAACTGTGG AAACCCCACC GCCAAACCCA CTATCAACGC CTTCATCAGC GGCTCCCAAA 
GCCTCACCAG CAAAAACTCC GGTTGGACCT CCTACAATAC CCGTTCGTAA CACCGTAGTT 
TACCATCCGC AAGACTTCCA GCAACGCATT CGTTCACAAC AAGCGCAACC ACCCAAACCG 
GTAGATACTC GCATCATCAA GCAGTACATA TGTCAGGGAA GGAAAATTTA CCTCACCAAA 
ATCAGTGTCA CAAAACCCAC ACAGGCCAGT TCCTCGAACG TTCAGCAGAC AGAGCCACCT 
CAGCAGTTCC TACAGTATCG GCAGCCCCAA ACGGACACGG GCTTTCATGG TTTCCCACAG 
GCAGCGATAA AATGCGATCA ACGCAATTTC CAGCAGTTCA ACGAAACGGT TCAGCAGTTG 
GAGCGGAGGA AAGTTCCAGA ACCTTCGGAT GAAAGCGGGA TGATTGAAAT TCAGAATATC 
CAGAAAATTC GTGCCAAGAT GCGAAATCGG AAACCGAGTG TGGTGACGGC TGTAACAACA 
CACGGCTTGA AAGGAGAAAC TTTCATCATT ACGGAACTGA ACGATCCGTC GCTGCATCCA 
ATGGAGATGC ATTCACCGGA AGTGAAGCAA GAAGTTCCGC TACAACATCC TCCTAGAATC 
ACCAGACGAA GAAATACCTT TGTAGTGGAT GATTCGGATG GAGACGTTCA AACCGATCAG 
TTACCACAGG TGGAAATGAC TAGTCAGGAT GATAGCCAAC AGGATGATAA ACCATCTGAA 
ATGGAACTCA AGGTGGAAGT CGAGAATGAG GAAAAGGAAC CGGTAACGAA CGCGGAACGA 
TTAGCATTCG ATCGGTGTAT GAAGGAAGAG GTACCATCTG GATCCGAATC CAGCCAGGCT 
GACGAAGATC TGGCACGGGA GATTTTGGCT CTGGTGGAAA ACGAACGCAA GGCTTTGCGC 
GCTGGGCCAA CGGTGAAGAA AGTCAAGAAG CGAAGAAAGC GACGTAAAAG GCACAAGAAG 
AAAAGTGATG GCAATGAGGA GTTGATGGAT CTCTTGATGA AGCATGTCAA TCAGGAGTCG 
TTAATTGCGG ATATCGTGGC CTCGCAGCAG AAGAAATTGC CTGTCATTGA GCTATCGGAT 
ACGGATGAGA ATGATGAGGA TAGAACAGTG CAGAGGAGAG TGACCTGCAA GGATGTTCGA 
GTGAAGCCAG ATCCTGATGC GGAAACAATC GGCGATAGGA AAACATCGTG TACGAGTGAC 
TTCGAGGAAT CTGAGACGGA TGACATTTTT GCCGAGATTT TGAAACCGTT TGTCGAGGAA 
GAGGAACATG CGGTTGGATC GGAAAGAGTT GTGGTGGAAG AAACGCCGCA GAAAGTTGTC 
TCAGGTGAAC CGATGGCTGA GTCGGATAAA CCATGTGCAA AGGAAATAAC TGGTTCAGAT 
GAGATTCTAA GTACGCTTGT CGAAAAAGAG AAACAAGCAG TTAGCCTTGA AGGCGTAGCG 
ATTCAAGAAT CGTTGCAGAA TGCTGTTCCA GGGGAATCAG AGGATGCATG TCCAGAGCAG 
CCGTCAGCAC CAGCGCAGAA AGCTACTTCA GAAGACATTT TGAAATCGAT TGTCGAGGAA 
GTGAAACAGT CGAATGAAGC GAAAGCTGTT CCTGAGAAAC CGGCACAAAA ATCGGATGAA 
GCATGTCGAG AAGCGCCGAG AGTACTGGCG GATGAAGTTA TTTCAATACC TGCAGTGGAA 
GAAATCGTTC CAGAGAATCA GGTAACATCA GAGGAACAGG ATGTTGTTCC AGAACAGCAA 
GCAGTAACAT TGAATAAGGT CGTTACCGAT GAGCAGAGAG AAACGATACC AGAAGAAAAG 
GCACTTTTAC CAGTGGAAGC CGCTCCGGCG GAACAAATAG TGCATGTGCC AAAAGAAGCC 
GAAACATCTG AGATTTCATC AAAATTAGTT ACGGAAGTCA GACCAGAGAT TGAATCGAAT 
CAACCTACCA TATGTGATCA AACCTGTGAT AACAGTGCCT TACCGGTCAA AGAGCTGCCG 
AAAAAAGCAC CTGCTCAAAG AGCACCCAAA CGCAAGCTCA AACCAACGTT AGTTTCTCGA 
TCAAAGATAA GAAAAGTTGA ACCGCCAGCA GCGATAGTCA CCAAAATCCA ACCACAACCA 
ACAAGAGTCG AAGAGTCAAC GGAAGTCAAC ATTGAAGATT CCAGTGATCC GTTCTCAGAT 
CGAGATGAGC CGCCACTACC GGATTCGGAG GAAGAGCGAA TCCAGTCTCA AATGGATACG 
ATCAACTCCA GAGTACGGGA ACTAGTCAAT GAGTGGGATT CTGATGAAGA AGAGACAAGT 
ATAAAGCAAC CATTGCAGAA ATATGCCTCG TTCGATAACA ACGGTAATCT TCCGTGTCGC 
ATTTCAAAAA ATAATGCGTT GAAACGTGCT GCGATACTGA GCGATCCGGT TTTGGAAGTT 
TCTGTTGAGG AAAGCCGCAA GGAGGAATCA CACGAAGCGA TTGAGGATAC TTCGGTGCAA 
GCTACAGCCA ACATCGTTAA GCCGGTAAGG GTTACCGTCC TAAAGGTGGA TTTGATAGAA 
CGATCAGAGA TCGTACAAGA GCCGGTAGAA CCCGCTACAA GAGAGAAGAC TCCGCCGCCA 
CCAACTGAAG CGAAGGGTCA AACCGTCGCG AATTTGTTGA AGGACTGGGA TGAATCACCG 
ATCAAACAAA AAAAGGAGAA CGCTCCAGTG GTTGCTCGGG TGCTATTAGA TAGACTCAGC 
TCATCTGAGT TAAATCAGTT GAAATCCAAA AAGGCTCCGG AAGTGTCAGA ATCAAGTAAG 
ATCACGATGG AACCATCGAA TCAACCACCA ACGATTGAAA GTAAGGCGAA GGATAGTCGA 
GATGAAATAA GCGGTGACGA ATCGGAGAGA GTCGTCGCTC GTATATTTAT GGGCGGACTA 
GACAGCTCTG GATCATCTGG ACAGCAACAA ATAGTGCCAC CAGCATCACA GATGGCGGCT 
CAAAGCTCTC CAGTTTCTAC TGGAAGTCCA AGCAAATCTC GTAAAAGTGA AAACAAGATT 
CCAAATACGG CCGAATCTCC TACCACTCGT TCGAAGTCTT CCAAGCAGAC ATCTATCAAA 
GATTGGATTC AAGGTTCTCC AACTACTGAG CCTTCGAAGC CTATTACGGG TAGGAGGAAA 
AGCTTGCGTG GAAGAGCCGC ATCTGTTCTA TTTGAACCAG AGGCTAACCA AGTCACTGAA 
AAACAGAAAC CTGCAGGCAG GAATCTGAGA AGCCGTACTA AATCCGTTTT TGCCGAAAAG 
CCACCTCTGA TGGAAGAAGA AGAACCGGTA CAGCAAGCAG ATATATCACG ACCAGTGGAG 
GAACCGTCTG AGAAACAGAT TGAAGTAACA CCGGAAAAGC CCAGCGGAAG TCGTCCGGAG 
GTGATAAAGG AAGCAGGGGG AACCGCTTGT GCGGCACGGA AAGAACAAGA ATCGAAAGAA 
TGCTCTACTA GCACTAAACC TACGCCGATC ATTCGGAACC GTGTTCCGAT TGCGATATCC 
TCGGAAGACG AAGATGATGC CCCGTTAGCC AAACGTCGCA AAGTGCTTCG CAAGAGGCGT 
TCCATGATGC GCCGTCCAAG CGCAATCCGG GATCTGAATG CTAACAAACG TCTGTTCCGA 
GGAGGCGCAA CTCTCAACAA TTCTCTCACC GTAGATGATT CCAACATGGG CGAAACTACC 
TTCATCAAAA GCGAACCTCC GGAGGAGATA GAAGATATTC AAGATTACTA TGCCTCTCCG 
AGTGAATCCT TTGCTGTCAC ATTCCCGGAT AATCCCTCCG AGCAACTGCT TTCCGTGGTC 
ACTGTGCAAA ACAATCAAGT AGTGCACTCA CAAGACTTCG ACGCTGGAAG TCCCTTCCGG 
AACCAGTATT TTGCCAAAGA TCTTATTCAA TCGGCAGCGA CTTCCACTCC TAACGCCCAA 
TCGCCGATCC AGCCTCCTCC TCCCGTACAG GATCATGTCT TTGCCACTCC GGAACCCGTC 
CGTGCGGCAA AACGGAAGCG TCGTACGAAA CTGGAAATGG AAAACGATCG AAGACTTGAA 
GCGATGAAAA GGGAAGAAGA GCAGAAGCCT GAAGAGCTCA AGAAAAAGGA AGCACAACGA 
GGCAGAGTGT TTAAATCCTT TACGCCAGTC GAGGTGAATG CCGTTGATCC GAATATTAGC 
CAGTTTGAAT GCGCTTCGTG TTTCGAGCAC GTTTCGCGTG ATGATTGGGA TGCTCACTAT 
GCGAAGCACA GTGGTCTTAC CTACCGAGTG AACGTAGATC CTGTGGTTGA CCTGAACGAC 
GAAACAACGG CCGTGGCAAT TGTGACACGT TTCATGAAGG CCCACCGGCA GGTGACGCTC 
TACTGTGACA AGTGCAATGT GGCCAAGAAA TCGGCCCTCG GATTAGTGTC GCATCGGCCT 
GTTTGTGGTC TGACGCGGCA AGAGATCCAA AACACCAAAA TACCGTGCCA GCATTGTGGC 
CGGAAGTTGA TGCCCGTCAG TATGAACTGT CACCTGTTGG GACATTGCGC GGTGCTGAAG 
CGAAAGAAAC AAGAAGAAGC TTTGGAGACG AAGCTGGAAG GAGCGTCGGC CGATGCGGAA 
ATGGAACCAA CAACGCTCAA TCAGCGTGGA ACGGTTAAGC GCCAAGCGAC CGCCAAGGCC 
GAGAGGCGCA TCCAGAACAT ATCGTTCAGC GAAATGATTC TCTCCGTCAC GAAGGACAAG 
GTTACCGAGG GTTGTTTGGC TGCTTGGAAA ACACAGTTCC GCAAGGCTAA GGTGGCGCTG 
TGCGTTTTTC GGACGTGTTC TTTCACTGGG ACCGACGAAA TAGAAATGCG CCACCATCAC 
GAGTTCTGTC CTTCGCCCTG TGAGCTACTC GAGTGCAAGA ATTGCAAGTT TCAAACGGAA 
AAGATCGACC AAATGGTGAA ACATATCAAG CTGAAACACA ACGACGTGCT GGTTTGTATG 
ACGATCGATG ATAGCAGCGG AACGGATGCC AAGATGACGT CGGAATCATC GGACGACGAG 
GGCGGTGCGG GTGTAGACGA AAACGATGAA CCATACATGA TTGGCATTGA CGATGAGGAC 
AAGCCGAAGA GGCGAAAAAT CAAAACTCTG CTGAAAACGT GCAAATTATC TACCAGGGAT 
ACGTTCAACG AGGAAAGCGA CGTTTATCGA GAGATGATCC TAGACGAAGT GATCAATCTT 
CGGCTGCTCA AGAGTGAGTT CCATCTGACG GCACGCGAAT GGACAGCGGA GTTCCGACGG 
TTGCATTATT CGACCAGCAT CCTGTTTTCA GCGTTACGAC CGGATTTGGA CGTACGGTTT 
CTTACGTTTA ACATGACCCA GGAGTACATC CCGAAGCAGA CACATTCTTT GCGGTTTGTC 
GCTCGGAGTA CGAATCTGTA CAACGCTCCC ATTCAACTGA AAGAATACTC CAACAAGTGG 
CAGAACTTGG AGGTTTTTAC CGGGCAGGTG AATGAGAAGG ACACGATATT CTTCTGTGGG 
GGACCCGTTG TTGCTCTAGA TTGGCTGCCA ATACCGGACG GCCAGCATGA ACATCAGATT 
GTGGCGGTGG CTTGCAAGAA CGATTTCGAA GAATTCTACC TGGCGGATCG CATCTTCCCG 
GTGAAGTGTT TGATTCAGAT CTGGGACGTG GGCTTACTGG ACAACAAAGA AATCTACAAC 
AAAAAAGCGG AGAATGCCAA ACCCACCTTA CTGTACTCCA TAGCGTGCGA CTTTGGTCCG 
ATTTGGGCGT TGAAGTTCTG CCCGAGTGGA TGTTACAACT CCAAGACCTC CGGCGACGAT 
TACGATCGGT TGGGCTTGCT TGCCGCGGCC GGGTCGGACG GAAATATTCA CATCTTTTCG 
CTGGGGCGCA GCTATGAACA CCTGATCTCG AACAGTAGCC GAGTGATCAA CTTGGCTCCG 
GTGCTCAAGC TGACTCTGTC CTTAGCGGAC GAAAGCTGTT GTTCGTCGTA TGAGTCCCAC 
AGTGCCGTGA AGTTGGCGTG GTCCAAGAGC AAACACCACT CGATGTTGGC AGCGGGCTAT 
TCGAACGGAA CGGTCGCAGT GTGGAATGCC ACGTCAACTT CGCCTCTGTT GACCGGAACG 
AAAGATAACG TTCGGGCACT GCTTCCCGTT CATAAGGTGT TCATTCCCGA TTCGTGCGTG 
ACCGCTGTCG ATCTTCATTA TTCGGAAGAT TCTCGTTATC TTTTGGTATG TAATGCGGAC 
AGGAAGGTCG TTGTCTACGA CCTGAACACG GGCTACATGC CGATGGAGAT TTGTAGTTTG 
AACGCCCGTA GTAAAGTTAC TACGGCTTGT TGGAATATCC ACTTCCCGCT GATAACGATG 
GCTTTCGACG ATGTGTATGC CATCGACCGG TGCGCTTTGA CGTTCCATCA GCCCCGTGAG 
ATTGGCGTGC GGTTGCATCC GTTGTATACT CATACGGCGG AAGCAACTGA CATGAGCAAC 
AGCGATCATC TGTCGACGCA CGTGATCGGT ACCGATGGCG GCGACGTGCT GAGCCACAAA 
CCGATGGCCT TTATTCACAA CATGGGCCAG AAGAATCCCG CGCAGATTAA ATATGTTTTG 
ACGTCAACAA TATCGGTGAA AGTCAATGAA GAACAAATGG ATAACTCTTA CAACAAGTTC 
GAGAAGAACT ATTGCGTGCT GTTTTCGGAC TTCGATAAGA ACCCGGCTCG AATGGACCTC 
AAATCGCTAC AGGTGAAAAC GTTCCGACGT GCCCTGCTGC ACGAATACCC GGGCATCCGG 
ATTAACCAGG TCAAGTGGAA TCCCAACGAC AAGTCCCATC AATACTACGC CATCGGGTAC 
CAGGCTGGCT TTGTGCGTGT GCGACCCTTC CGGCTGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568954 ( XM_021854098.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709790.1 Aedes aegypti titin (LOC5568954), transcript variant X4, mRNA.

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CloneID	OAe129075
Clone ID Related Accession (Same CDS sequence)	XM_021854098.1 , XM_021854096.1 , XM_021854097.1 , XM_021854099.1 , XM_021854100.1 , XM_021854095.1
Accession Version	XM_021854098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	titin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709790.1
CDS	643..11598
Translation	MDTTSAAAALNPGEGSNFGVVHVKQEPGFSGESSPGPEGSIGTDGGDGARTIRKITVLDPLKLKLLSRQNHNKLLSGLNAAKAKAQPPAPKPPPLAAPSSSASTVTEKETPSEVIATDRTVEAESEPLRPDVEEPPKQTSSTSSAPPKTGAGGGSTLFRRKKVVPVAIARKLAQKEKTKPAPKQCEEKTPPATSPTDASPVQCEPVLPEPVEQVPQQPESAPVEPKPVEPESSQHEAKEPAASSEPNPVEQTETREKPVASPPPLVLVKREPSDLVTRPTAFRRIKPKVAICLNPSRPKKSILKKTTSADKLLPAGSNEPSKSFKDLIPEETIVLDDDDEEDNEQHTPSVTVEEADHTGKSEVQRTQQEEAERMAEEETRRIAEEQRQKDAEDQRRIEEGARRKAEEQRRILAEQKKREEEEKRRIAEDQRRAEEEAKKVAEEQCRIQEEMKKREEEQRQKLEQLRIQEEQRKVAEEQKRLEMLEQEEKAKRVEPEKPAAESRKTMKIPPEIWKRQMERMKNNPNRQHSTVPAIPQSNAEPSQSQHTITSDPVNVKTSNVVEQEPVSVVAPAVNQERANQISEKPAVQVTPNPTKEIRRISRLPPSLAMKLMNCVALKPSRNKIEEKAAPSVKPSQNKTETSHATKEVPSSAPAVVSQTAIRRISGLSPSASMTPIKTAEAMQQEVQETTADKQQQSIVPESAVANVERTEKTEPVERTVISNKPAPTKEIKRIALPPDIALKLLGAVSRNKPVVVTSTAGTVDPAKNVASKPATIAEKPAVAEPESSNPTVTAAAPIVVPPIKQTTIQSGTNAPKEIRRISQLPPNVAMRLVDSLKQKGITVPKLCLKKDTPTIEPVSVAASDVQLPSEPSTPSSVASVPEIKVEPQEQPTKSKGKVEILSQVILPTASYNLEELLAGKSTEGFAPSCAMPLPLPMHKGRRQAAPVPIAPVPTSIHSEPIQHSQVSLPVQQPSKPETPAPVTAVPSPIPDLPVAMAENDIASEVTVTTENEDIPNPSHEKFDEYIISYSDCEGAPVQYDPSKSAQQQNKEEIPPEPNVTFVSSNAPTVMSHQYSVTYSANSHQQLHRPNVQAPIVIQQPANVSSYQPRPVAYFTKVSNNTQRPSPQPTPVTYHPQFTVLSQPGMNPTRMISQRMIINPAASMQSGQRPRYPTAQFVVLRHPTPIRGQLVAQTQLRQEHPQPVPQQSQQHLTVPRETYYHRLPIVNQNQPSVIVPTPRYVIPKQYPQIQMARPPRPQRPSVDIAQNQSKAFQPVQQSQPTTAFSIAQPVQQQRVIPKIVIDSSYVDQQERGTPASSNTPPLQDQWKTQNTAPCSEEIRNKLRDKLKSRMLQQSAKSGSPTVETPPPNPLSTPSSAAPKASPAKTPVGPPTIPVRNTVVYHPQDFQQRIRSQQAQPPKPVDTRIIKQYICQGRKIYLTKISVTKPTQASSSNVQQTEPPQQFLQYRQPQTDTGFHGFPQAAIKCDQRNFQQFNETVQQLERRKVPEPSDESGMIEIQNIQKIRAKMRNRKPSVVTAVTTHGLKGETFIITELNDPSLHPMEMHSPEVKQEVPLQHPPRITRRRNTFVVDDSDGDVQTDQLPQVEMTSQDDSQQDDKPSEMELKVEVENEEKEPVTNAERLAFDRCMKEEVPSGSESSQADEDLAREILALVENERKALRAGPTVKKVKKRRKRRKRHKKKSDGNEELMDLLMKHVNQESLIADIVASQQKKLPVIELSDTDENDEDRTVQRRVTCKDVRVKPDPDAETIGDRKTSCTSDFEESETNDIFAEILKPFVEEEEHAVGSERVVVEETPQKVVSGKPMAESDKACAKEIAGSDEILSTLVEKEKQAVSLKSVATQESLQNAVPWESEDACPEKPSAPAQKATSDDILKSIVEEVKQSNEAEAVPEKPAQKSDEACREAPRVLADEVISIPAVEEIVPENQVTSEEQDVVPEQQAVTLNEMVTDEQRETIPEEKALLPVEAAPAEQIVHVPKQAETSEISSKLVTEVRPEIESNQPTICDQTCDNSALPVKELPKKAPAQRAPKRKLKPTLVSRSKIRKVEPPAAIVTKIQPQPTRVEKSTEVNIEDSSDPFSDRDKPPLPDSEEERIQSQMDTINSRVRELVNEWDSDEEETSIKQPLQKYASFDNNGNLPCRISKNNALKRAAILSDPVLEVSVEESRKEESHEAIEDTSVQATANIVKPVRVTVLKVDLIERSEIVQEPVEPATREKTPPPPTEAKGQTVANLLKDWDESPIKQKKENAPVVARVLLDRLSSSELNQLKSKKAPEVSESSKITMEPSNQSPTIESKAKDSRDEISGDESERVVARIFMGGLDSSGSSGQQQIVPPASQMAAQSSPVSTGSPSKSRKSENKIPNTAESPTTRSKSSKQTSIKDWIQGSPTTEPSKPITGRRKGLRGRAASVLFEPEANQVTEKQKPAGRNLRSRTKSVFAEKPPLMEEEEPVQQEDISRPVEEPSEEQIEVTPEKPSGSRPEVIKEAGGTACAARKEQESKEYSTSTKPTPIIRNRVPIAISSEDEDDAPLAKRRKVLRKRRSMMRRPSAIRDLNANKRLFRGGATLNNSLIVDDSNMGETTFIKSEPPEEIEDIQDYYASPSESFAVTFPDNPSEQLLSVVTVQNNQVVHSQDFDAGSPFRNQYFAKDLIQSAATSTPNAQSPIQPPPPVQDHVFATPEPVRAAKRKRRTKLEMENDRRLEAMKREEEQKPEELKKKEAQRGRVFKSFTPVKVDAVDPNISQFECASCFEHVSRDDWDAHYAKHSGLTYRVNVDPVVDLNDETTAVAIVTRFMKAHRQVTLYCDKCNVAKKSALGLVSHRPVCGLTRQEILNTKIPCQHCGRKLMPVSMNCHLLGHCAVLKRKKQEEALETKLEGASADVEMEPTTLNQRGTVKRQATAKAERRIQNISFSEMILSVTKDKVTEGCLAAWKTQFRKAKVALCVFRTCSFTGTDEIEMRHHHEFCPSPCELLECKNCKFQTEKIDQMVKHIKLKHNDVLVCMTIDDSSGTDAKMTSESSDDEGGTGVDENDEPYMIGIDDEDKPKKRKIKTLLKTCKLSTRDTFNEESDVYREMILDEVINLRLLKSEFHLTAREWTAEFRRLHYSTSILFSALRPDLDVRFLTFNMTQEYIPKQTHSLRFVARSTNLYNAPIQLKEYANKWQNLEVFTGQVNEKDTIFFCGGPVVALDWLPIPDGQHEHQIVAVACKNDFEEFYLADRIIPVKCLIQIWDVGLLDNKEIYNKKAENAKPTLLYSIACDVGPIWALKFCPSGCYNSKTSGDDYDRLGLLAAAGSDGNIHIFSLGRSYEHLISNSSRVINLAPVLKLTLSLADQSCCSSYESHSAVKLAWSKSKHHSMLAAGYSNGTVAVWNATSTSPLLTGTKDNVRALLPVHKVFIPDSCVTAVDLHYSEDSRYLLVCNADRKVVVYDLNTGYMPMEICSLNARSKVTTACWNIHFPLITMAFDDVYAIDRCALTFHQPREIGVRLHPLYTHTAEATDMSNSDHLSTHVIGTDGGDVLSHKPMAFIHNMGQKNPAQIKYVLTSTISVKVNEEQMDNSYNKFEKNYCVLFSDFDKNPARMDLKSLQVKTFRRALLHEYPGIRINQVKWNPNDKSHQYYAIGYQAGFVRVRPFRLK

Target ORF information:

RefSeq Version	XM_021854098.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti titin (LOC5568954), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021854098.1

ORF Insert Sequence:

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568954 ( XM_021854096.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709788.1 Aedes aegypti titin (LOC5568954), transcript variant X2, mRNA.

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CloneID	OAe129075
Clone ID Related Accession (Same CDS sequence)	XM_021854098.1 , XM_021854096.1 , XM_021854097.1 , XM_021854099.1 , XM_021854100.1 , XM_021854095.1
Accession Version	XM_021854096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	titin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709788.1
CDS	356..11311
Translation	MDTTSAAAALNPGEGSNFGVVHVKQEPGFSGESSPGPEGSIGTDGGDGARTIRKITVLDPLKLKLLSRQNHNKLLSGLNAAKAKAQPPAPKPPPLAAPSSSASTVTEKETPSEVIATDRTVEAESEPLRPDVEEPPKQTSSTSSAPPKTGAGGGSTLFRRKKVVPVAIARKLAQKEKTKPAPKQCEEKTPPATSPTDASPVQCEPVLPEPVEQVPQQPESAPVEPKPVEPESSQHEAKEPAASSEPNPVEQTETREKPVASPPPLVLVKREPSDLVTRPTAFRRIKPKVAICLNPSRPKKSILKKTTSADKLLPAGSNEPSKSFKDLIPEETIVLDDDDEEDNEQHTPSVTVEEADHTGKSEVQRTQQEEAERMAEEETRRIAEEQRQKDAEDQRRIEEGARRKAEEQRRILAEQKKREEEEKRRIAEDQRRAEEEAKKVAEEQCRIQEEMKKREEEQRQKLEQLRIQEEQRKVAEEQKRLEMLEQEEKAKRVEPEKPAAESRKTMKIPPEIWKRQMERMKNNPNRQHSTVPAIPQSNAEPSQSQHTITSDPVNVKTSNVVEQEPVSVVAPAVNQERANQISEKPAVQVTPNPTKEIRRISRLPPSLAMKLMNCVALKPSRNKIEEKAAPSVKPSQNKTETSHATKEVPSSAPAVVSQTAIRRISGLSPSASMTPIKTAEAMQQEVQETTADKQQQSIVPESAVANVERTEKTEPVERTVISNKPAPTKEIKRIALPPDIALKLLGAVSRNKPVVVTSTAGTVDPAKNVASKPATIAEKPAVAEPESSNPTVTAAAPIVVPPIKQTTIQSGTNAPKEIRRISQLPPNVAMRLVDSLKQKGITVPKLCLKKDTPTIEPVSVAASDVQLPSEPSTPSSVASVPEIKVEPQEQPTKSKGKVEILSQVILPTASYNLEELLAGKSTEGFAPSCAMPLPLPMHKGRRQAAPVPIAPVPTSIHSEPIQHSQVSLPVQQPSKPETPAPVTAVPSPIPDLPVAMAENDIASEVTVTTENEDIPNPSHEKFDEYIISYSDCEGAPVQYDPSKSAQQQNKEEIPPEPNVTFVSSNAPTVMSHQYSVTYSANSHQQLHRPNVQAPIVIQQPANVSSYQPRPVAYFTKVSNNTQRPSPQPTPVTYHPQFTVLSQPGMNPTRMISQRMIINPAASMQSGQRPRYPTAQFVVLRHPTPIRGQLVAQTQLRQEHPQPVPQQSQQHLTVPRETYYHRLPIVNQNQPSVIVPTPRYVIPKQYPQIQMARPPRPQRPSVDIAQNQSKAFQPVQQSQPTTAFSIAQPVQQQRVIPKIVIDSSYVDQQERGTPASSNTPPLQDQWKTQNTAPCSEEIRNKLRDKLKSRMLQQSAKSGSPTVETPPPNPLSTPSSAAPKASPAKTPVGPPTIPVRNTVVYHPQDFQQRIRSQQAQPPKPVDTRIIKQYICQGRKIYLTKISVTKPTQASSSNVQQTEPPQQFLQYRQPQTDTGFHGFPQAAIKCDQRNFQQFNETVQQLERRKVPEPSDESGMIEIQNIQKIRAKMRNRKPSVVTAVTTHGLKGETFIITELNDPSLHPMEMHSPEVKQEVPLQHPPRITRRRNTFVVDDSDGDVQTDQLPQVEMTSQDDSQQDDKPSEMELKVEVENEEKEPVTNAERLAFDRCMKEEVPSGSESSQADEDLAREILALVENERKALRAGPTVKKVKKRRKRRKRHKKKSDGNEELMDLLMKHVNQESLIADIVASQQKKLPVIELSDTDENDEDRTVQRRVTCKDVRVKPDPDAETIGDRKTSCTSDFEESETNDIFAEILKPFVEEEEHAVGSERVVVEETPQKVVSGKPMAESDKACAKEIAGSDEILSTLVEKEKQAVSLKSVATQESLQNAVPWESEDACPEKPSAPAQKATSDDILKSIVEEVKQSNEAEAVPEKPAQKSDEACREAPRVLADEVISIPAVEEIVPENQVTSEEQDVVPEQQAVTLNEMVTDEQRETIPEEKALLPVEAAPAEQIVHVPKQAETSEISSKLVTEVRPEIESNQPTICDQTCDNSALPVKELPKKAPAQRAPKRKLKPTLVSRSKIRKVEPPAAIVTKIQPQPTRVEKSTEVNIEDSSDPFSDRDKPPLPDSEEERIQSQMDTINSRVRELVNEWDSDEEETSIKQPLQKYASFDNNGNLPCRISKNNALKRAAILSDPVLEVSVEESRKEESHEAIEDTSVQATANIVKPVRVTVLKVDLIERSEIVQEPVEPATREKTPPPPTEAKGQTVANLLKDWDESPIKQKKENAPVVARVLLDRLSSSELNQLKSKKAPEVSESSKITMEPSNQSPTIESKAKDSRDEISGDESERVVARIFMGGLDSSGSSGQQQIVPPASQMAAQSSPVSTGSPSKSRKSENKIPNTAESPTTRSKSSKQTSIKDWIQGSPTTEPSKPITGRRKGLRGRAASVLFEPEANQVTEKQKPAGRNLRSRTKSVFAEKPPLMEEEEPVQQEDISRPVEEPSEEQIEVTPEKPSGSRPEVIKEAGGTACAARKEQESKEYSTSTKPTPIIRNRVPIAISSEDEDDAPLAKRRKVLRKRRSMMRRPSAIRDLNANKRLFRGGATLNNSLIVDDSNMGETTFIKSEPPEEIEDIQDYYASPSESFAVTFPDNPSEQLLSVVTVQNNQVVHSQDFDAGSPFRNQYFAKDLIQSAATSTPNAQSPIQPPPPVQDHVFATPEPVRAAKRKRRTKLEMENDRRLEAMKREEEQKPEELKKKEAQRGRVFKSFTPVKVDAVDPNISQFECASCFEHVSRDDWDAHYAKHSGLTYRVNVDPVVDLNDETTAVAIVTRFMKAHRQVTLYCDKCNVAKKSALGLVSHRPVCGLTRQEILNTKIPCQHCGRKLMPVSMNCHLLGHCAVLKRKKQEEALETKLEGASADVEMEPTTLNQRGTVKRQATAKAERRIQNISFSEMILSVTKDKVTEGCLAAWKTQFRKAKVALCVFRTCSFTGTDEIEMRHHHEFCPSPCELLECKNCKFQTEKIDQMVKHIKLKHNDVLVCMTIDDSSGTDAKMTSESSDDEGGTGVDENDEPYMIGIDDEDKPKKRKIKTLLKTCKLSTRDTFNEESDVYREMILDEVINLRLLKSEFHLTAREWTAEFRRLHYSTSILFSALRPDLDVRFLTFNMTQEYIPKQTHSLRFVARSTNLYNAPIQLKEYANKWQNLEVFTGQVNEKDTIFFCGGPVVALDWLPIPDGQHEHQIVAVACKNDFEEFYLADRIIPVKCLIQIWDVGLLDNKEIYNKKAENAKPTLLYSIACDVGPIWALKFCPSGCYNSKTSGDDYDRLGLLAAAGSDGNIHIFSLGRSYEHLISNSSRVINLAPVLKLTLSLADQSCCSSYESHSAVKLAWSKSKHHSMLAAGYSNGTVAVWNATSTSPLLTGTKDNVRALLPVHKVFIPDSCVTAVDLHYSEDSRYLLVCNADRKVVVYDLNTGYMPMEICSLNARSKVTTACWNIHFPLITMAFDDVYAIDRCALTFHQPREIGVRLHPLYTHTAEATDMSNSDHLSTHVIGTDGGDVLSHKPMAFIHNMGQKNPAQIKYVLTSTISVKVNEEQMDNSYNKFEKNYCVLFSDFDKNPARMDLKSLQVKTFRRALLHEYPGIRINQVKWNPNDKSHQYYAIGYQAGFVRVRPFRLK

Target ORF information:

RefSeq Version	XM_021854096.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti titin (LOC5568954), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021854096.1

ORF Insert Sequence:

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568954 ( XM_021854097.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709789.1 Aedes aegypti titin (LOC5568954), transcript variant X3, mRNA.

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CloneID	OAe129075
Clone ID Related Accession (Same CDS sequence)	XM_021854098.1 , XM_021854096.1 , XM_021854097.1 , XM_021854099.1 , XM_021854100.1 , XM_021854095.1
Accession Version	XM_021854097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	titin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709789.1
CDS	341..11296
Translation	MDTTSAAAALNPGEGSNFGVVHVKQEPGFSGESSPGPEGSIGTDGGDGARTIRKITVLDPLKLKLLSRQNHNKLLSGLNAAKAKAQPPAPKPPPLAAPSSSASTVTEKETPSEVIATDRTVEAESEPLRPDVEEPPKQTSSTSSAPPKTGAGGGSTLFRRKKVVPVAIARKLAQKEKTKPAPKQCEEKTPPATSPTDASPVQCEPVLPEPVEQVPQQPESAPVEPKPVEPESSQHEAKEPAASSEPNPVEQTETREKPVASPPPLVLVKREPSDLVTRPTAFRRIKPKVAICLNPSRPKKSILKKTTSADKLLPAGSNEPSKSFKDLIPEETIVLDDDDEEDNEQHTPSVTVEEADHTGKSEVQRTQQEEAERMAEEETRRIAEEQRQKDAEDQRRIEEGARRKAEEQRRILAEQKKREEEEKRRIAEDQRRAEEEAKKVAEEQCRIQEEMKKREEEQRQKLEQLRIQEEQRKVAEEQKRLEMLEQEEKAKRVEPEKPAAESRKTMKIPPEIWKRQMERMKNNPNRQHSTVPAIPQSNAEPSQSQHTITSDPVNVKTSNVVEQEPVSVVAPAVNQERANQISEKPAVQVTPNPTKEIRRISRLPPSLAMKLMNCVALKPSRNKIEEKAAPSVKPSQNKTETSHATKEVPSSAPAVVSQTAIRRISGLSPSASMTPIKTAEAMQQEVQETTADKQQQSIVPESAVANVERTEKTEPVERTVISNKPAPTKEIKRIALPPDIALKLLGAVSRNKPVVVTSTAGTVDPAKNVASKPATIAEKPAVAEPESSNPTVTAAAPIVVPPIKQTTIQSGTNAPKEIRRISQLPPNVAMRLVDSLKQKGITVPKLCLKKDTPTIEPVSVAASDVQLPSEPSTPSSVASVPEIKVEPQEQPTKSKGKVEILSQVILPTASYNLEELLAGKSTEGFAPSCAMPLPLPMHKGRRQAAPVPIAPVPTSIHSEPIQHSQVSLPVQQPSKPETPAPVTAVPSPIPDLPVAMAENDIASEVTVTTENEDIPNPSHEKFDEYIISYSDCEGAPVQYDPSKSAQQQNKEEIPPEPNVTFVSSNAPTVMSHQYSVTYSANSHQQLHRPNVQAPIVIQQPANVSSYQPRPVAYFTKVSNNTQRPSPQPTPVTYHPQFTVLSQPGMNPTRMISQRMIINPAASMQSGQRPRYPTAQFVVLRHPTPIRGQLVAQTQLRQEHPQPVPQQSQQHLTVPRETYYHRLPIVNQNQPSVIVPTPRYVIPKQYPQIQMARPPRPQRPSVDIAQNQSKAFQPVQQSQPTTAFSIAQPVQQQRVIPKIVIDSSYVDQQERGTPASSNTPPLQDQWKTQNTAPCSEEIRNKLRDKLKSRMLQQSAKSGSPTVETPPPNPLSTPSSAAPKASPAKTPVGPPTIPVRNTVVYHPQDFQQRIRSQQAQPPKPVDTRIIKQYICQGRKIYLTKISVTKPTQASSSNVQQTEPPQQFLQYRQPQTDTGFHGFPQAAIKCDQRNFQQFNETVQQLERRKVPEPSDESGMIEIQNIQKIRAKMRNRKPSVVTAVTTHGLKGETFIITELNDPSLHPMEMHSPEVKQEVPLQHPPRITRRRNTFVVDDSDGDVQTDQLPQVEMTSQDDSQQDDKPSEMELKVEVENEEKEPVTNAERLAFDRCMKEEVPSGSESSQADEDLAREILALVENERKALRAGPTVKKVKKRRKRRKRHKKKSDGNEELMDLLMKHVNQESLIADIVASQQKKLPVIELSDTDENDEDRTVQRRVTCKDVRVKPDPDAETIGDRKTSCTSDFEESETNDIFAEILKPFVEEEEHAVGSERVVVEETPQKVVSGKPMAESDKACAKEIAGSDEILSTLVEKEKQAVSLKSVATQESLQNAVPWESEDACPEKPSAPAQKATSDDILKSIVEEVKQSNEAEAVPEKPAQKSDEACREAPRVLADEVISIPAVEEIVPENQVTSEEQDVVPEQQAVTLNEMVTDEQRETIPEEKALLPVEAAPAEQIVHVPKQAETSEISSKLVTEVRPEIESNQPTICDQTCDNSALPVKELPKKAPAQRAPKRKLKPTLVSRSKIRKVEPPAAIVTKIQPQPTRVEKSTEVNIEDSSDPFSDRDKPPLPDSEEERIQSQMDTINSRVRELVNEWDSDEEETSIKQPLQKYASFDNNGNLPCRISKNNALKRAAILSDPVLEVSVEESRKEESHEAIEDTSVQATANIVKPVRVTVLKVDLIERSEIVQEPVEPATREKTPPPPTEAKGQTVANLLKDWDESPIKQKKENAPVVARVLLDRLSSSELNQLKSKKAPEVSESSKITMEPSNQSPTIESKAKDSRDEISGDESERVVARIFMGGLDSSGSSGQQQIVPPASQMAAQSSPVSTGSPSKSRKSENKIPNTAESPTTRSKSSKQTSIKDWIQGSPTTEPSKPITGRRKGLRGRAASVLFEPEANQVTEKQKPAGRNLRSRTKSVFAEKPPLMEEEEPVQQEDISRPVEEPSEEQIEVTPEKPSGSRPEVIKEAGGTACAARKEQESKEYSTSTKPTPIIRNRVPIAISSEDEDDAPLAKRRKVLRKRRSMMRRPSAIRDLNANKRLFRGGATLNNSLIVDDSNMGETTFIKSEPPEEIEDIQDYYASPSESFAVTFPDNPSEQLLSVVTVQNNQVVHSQDFDAGSPFRNQYFAKDLIQSAATSTPNAQSPIQPPPPVQDHVFATPEPVRAAKRKRRTKLEMENDRRLEAMKREEEQKPEELKKKEAQRGRVFKSFTPVKVDAVDPNISQFECASCFEHVSRDDWDAHYAKHSGLTYRVNVDPVVDLNDETTAVAIVTRFMKAHRQVTLYCDKCNVAKKSALGLVSHRPVCGLTRQEILNTKIPCQHCGRKLMPVSMNCHLLGHCAVLKRKKQEEALETKLEGASADVEMEPTTLNQRGTVKRQATAKAERRIQNISFSEMILSVTKDKVTEGCLAAWKTQFRKAKVALCVFRTCSFTGTDEIEMRHHHEFCPSPCELLECKNCKFQTEKIDQMVKHIKLKHNDVLVCMTIDDSSGTDAKMTSESSDDEGGTGVDENDEPYMIGIDDEDKPKKRKIKTLLKTCKLSTRDTFNEESDVYREMILDEVINLRLLKSEFHLTAREWTAEFRRLHYSTSILFSALRPDLDVRFLTFNMTQEYIPKQTHSLRFVARSTNLYNAPIQLKEYANKWQNLEVFTGQVNEKDTIFFCGGPVVALDWLPIPDGQHEHQIVAVACKNDFEEFYLADRIIPVKCLIQIWDVGLLDNKEIYNKKAENAKPTLLYSIACDVGPIWALKFCPSGCYNSKTSGDDYDRLGLLAAAGSDGNIHIFSLGRSYEHLISNSSRVINLAPVLKLTLSLADQSCCSSYESHSAVKLAWSKSKHHSMLAAGYSNGTVAVWNATSTSPLLTGTKDNVRALLPVHKVFIPDSCVTAVDLHYSEDSRYLLVCNADRKVVVYDLNTGYMPMEICSLNARSKVTTACWNIHFPLITMAFDDVYAIDRCALTFHQPREIGVRLHPLYTHTAEATDMSNSDHLSTHVIGTDGGDVLSHKPMAFIHNMGQKNPAQIKYVLTSTISVKVNEEQMDNSYNKFEKNYCVLFSDFDKNPARMDLKSLQVKTFRRALLHEYPGIRINQVKWNPNDKSHQYYAIGYQAGFVRVRPFRLK

Target ORF information:

RefSeq Version	XM_021854097.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti titin (LOC5568954), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021854097.1

ORF Insert Sequence:

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568954 ( XM_021854099.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709791.1 Aedes aegypti titin (LOC5568954), transcript variant X5, mRNA.

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CloneID	OAe129075
Clone ID Related Accession (Same CDS sequence)	XM_021854098.1 , XM_021854096.1 , XM_021854097.1 , XM_021854099.1 , XM_021854100.1 , XM_021854095.1
Accession Version	XM_021854099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	titin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709791.1
CDS	300..11255
Translation	MDTTSAAAALNPGEGSNFGVVHVKQEPGFSGESSPGPEGSIGTDGGDGARTIRKITVLDPLKLKLLSRQNHNKLLSGLNAAKAKAQPPAPKPPPLAAPSSSASTVTEKETPSEVIATDRTVEAESEPLRPDVEEPPKQTSSTSSAPPKTGAGGGSTLFRRKKVVPVAIARKLAQKEKTKPAPKQCEEKTPPATSPTDASPVQCEPVLPEPVEQVPQQPESAPVEPKPVEPESSQHEAKEPAASSEPNPVEQTETREKPVASPPPLVLVKREPSDLVTRPTAFRRIKPKVAICLNPSRPKKSILKKTTSADKLLPAGSNEPSKSFKDLIPEETIVLDDDDEEDNEQHTPSVTVEEADHTGKSEVQRTQQEEAERMAEEETRRIAEEQRQKDAEDQRRIEEGARRKAEEQRRILAEQKKREEEEKRRIAEDQRRAEEEAKKVAEEQCRIQEEMKKREEEQRQKLEQLRIQEEQRKVAEEQKRLEMLEQEEKAKRVEPEKPAAESRKTMKIPPEIWKRQMERMKNNPNRQHSTVPAIPQSNAEPSQSQHTITSDPVNVKTSNVVEQEPVSVVAPAVNQERANQISEKPAVQVTPNPTKEIRRISRLPPSLAMKLMNCVALKPSRNKIEEKAAPSVKPSQNKTETSHATKEVPSSAPAVVSQTAIRRISGLSPSASMTPIKTAEAMQQEVQETTADKQQQSIVPESAVANVERTEKTEPVERTVISNKPAPTKEIKRIALPPDIALKLLGAVSRNKPVVVTSTAGTVDPAKNVASKPATIAEKPAVAEPESSNPTVTAAAPIVVPPIKQTTIQSGTNAPKEIRRISQLPPNVAMRLVDSLKQKGITVPKLCLKKDTPTIEPVSVAASDVQLPSEPSTPSSVASVPEIKVEPQEQPTKSKGKVEILSQVILPTASYNLEELLAGKSTEGFAPSCAMPLPLPMHKGRRQAAPVPIAPVPTSIHSEPIQHSQVSLPVQQPSKPETPAPVTAVPSPIPDLPVAMAENDIASEVTVTTENEDIPNPSHEKFDEYIISYSDCEGAPVQYDPSKSAQQQNKEEIPPEPNVTFVSSNAPTVMSHQYSVTYSANSHQQLHRPNVQAPIVIQQPANVSSYQPRPVAYFTKVSNNTQRPSPQPTPVTYHPQFTVLSQPGMNPTRMISQRMIINPAASMQSGQRPRYPTAQFVVLRHPTPIRGQLVAQTQLRQEHPQPVPQQSQQHLTVPRETYYHRLPIVNQNQPSVIVPTPRYVIPKQYPQIQMARPPRPQRPSVDIAQNQSKAFQPVQQSQPTTAFSIAQPVQQQRVIPKIVIDSSYVDQQERGTPASSNTPPLQDQWKTQNTAPCSEEIRNKLRDKLKSRMLQQSAKSGSPTVETPPPNPLSTPSSAAPKASPAKTPVGPPTIPVRNTVVYHPQDFQQRIRSQQAQPPKPVDTRIIKQYICQGRKIYLTKISVTKPTQASSSNVQQTEPPQQFLQYRQPQTDTGFHGFPQAAIKCDQRNFQQFNETVQQLERRKVPEPSDESGMIEIQNIQKIRAKMRNRKPSVVTAVTTHGLKGETFIITELNDPSLHPMEMHSPEVKQEVPLQHPPRITRRRNTFVVDDSDGDVQTDQLPQVEMTSQDDSQQDDKPSEMELKVEVENEEKEPVTNAERLAFDRCMKEEVPSGSESSQADEDLAREILALVENERKALRAGPTVKKVKKRRKRRKRHKKKSDGNEELMDLLMKHVNQESLIADIVASQQKKLPVIELSDTDENDEDRTVQRRVTCKDVRVKPDPDAETIGDRKTSCTSDFEESETNDIFAEILKPFVEEEEHAVGSERVVVEETPQKVVSGKPMAESDKACAKEIAGSDEILSTLVEKEKQAVSLKSVATQESLQNAVPWESEDACPEKPSAPAQKATSDDILKSIVEEVKQSNEAEAVPEKPAQKSDEACREAPRVLADEVISIPAVEEIVPENQVTSEEQDVVPEQQAVTLNEMVTDEQRETIPEEKALLPVEAAPAEQIVHVPKQAETSEISSKLVTEVRPEIESNQPTICDQTCDNSALPVKELPKKAPAQRAPKRKLKPTLVSRSKIRKVEPPAAIVTKIQPQPTRVEKSTEVNIEDSSDPFSDRDKPPLPDSEEERIQSQMDTINSRVRELVNEWDSDEEETSIKQPLQKYASFDNNGNLPCRISKNNALKRAAILSDPVLEVSVEESRKEESHEAIEDTSVQATANIVKPVRVTVLKVDLIERSEIVQEPVEPATREKTPPPPTEAKGQTVANLLKDWDESPIKQKKENAPVVARVLLDRLSSSELNQLKSKKAPEVSESSKITMEPSNQSPTIESKAKDSRDEISGDESERVVARIFMGGLDSSGSSGQQQIVPPASQMAAQSSPVSTGSPSKSRKSENKIPNTAESPTTRSKSSKQTSIKDWIQGSPTTEPSKPITGRRKGLRGRAASVLFEPEANQVTEKQKPAGRNLRSRTKSVFAEKPPLMEEEEPVQQEDISRPVEEPSEEQIEVTPEKPSGSRPEVIKEAGGTACAARKEQESKEYSTSTKPTPIIRNRVPIAISSEDEDDAPLAKRRKVLRKRRSMMRRPSAIRDLNANKRLFRGGATLNNSLIVDDSNMGETTFIKSEPPEEIEDIQDYYASPSESFAVTFPDNPSEQLLSVVTVQNNQVVHSQDFDAGSPFRNQYFAKDLIQSAATSTPNAQSPIQPPPPVQDHVFATPEPVRAAKRKRRTKLEMENDRRLEAMKREEEQKPEELKKKEAQRGRVFKSFTPVKVDAVDPNISQFECASCFEHVSRDDWDAHYAKHSGLTYRVNVDPVVDLNDETTAVAIVTRFMKAHRQVTLYCDKCNVAKKSALGLVSHRPVCGLTRQEILNTKIPCQHCGRKLMPVSMNCHLLGHCAVLKRKKQEEALETKLEGASADVEMEPTTLNQRGTVKRQATAKAERRIQNISFSEMILSVTKDKVTEGCLAAWKTQFRKAKVALCVFRTCSFTGTDEIEMRHHHEFCPSPCELLECKNCKFQTEKIDQMVKHIKLKHNDVLVCMTIDDSSGTDAKMTSESSDDEGGTGVDENDEPYMIGIDDEDKPKKRKIKTLLKTCKLSTRDTFNEESDVYREMILDEVINLRLLKSEFHLTAREWTAEFRRLHYSTSILFSALRPDLDVRFLTFNMTQEYIPKQTHSLRFVARSTNLYNAPIQLKEYANKWQNLEVFTGQVNEKDTIFFCGGPVVALDWLPIPDGQHEHQIVAVACKNDFEEFYLADRIIPVKCLIQIWDVGLLDNKEIYNKKAENAKPTLLYSIACDVGPIWALKFCPSGCYNSKTSGDDYDRLGLLAAAGSDGNIHIFSLGRSYEHLISNSSRVINLAPVLKLTLSLADQSCCSSYESHSAVKLAWSKSKHHSMLAAGYSNGTVAVWNATSTSPLLTGTKDNVRALLPVHKVFIPDSCVTAVDLHYSEDSRYLLVCNADRKVVVYDLNTGYMPMEICSLNARSKVTTACWNIHFPLITMAFDDVYAIDRCALTFHQPREIGVRLHPLYTHTAEATDMSNSDHLSTHVIGTDGGDVLSHKPMAFIHNMGQKNPAQIKYVLTSTISVKVNEEQMDNSYNKFEKNYCVLFSDFDKNPARMDLKSLQVKTFRRALLHEYPGIRINQVKWNPNDKSHQYYAIGYQAGFVRVRPFRLK

Target ORF information:

RefSeq Version	XM_021854099.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti titin (LOC5568954), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021854099.1

ORF Insert Sequence:

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568954 ( XM_021854100.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709792.1 Aedes aegypti titin (LOC5568954), transcript variant X6, mRNA.

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CloneID	OAe129075
Clone ID Related Accession (Same CDS sequence)	XM_021854098.1 , XM_021854096.1 , XM_021854097.1 , XM_021854099.1 , XM_021854100.1 , XM_021854095.1
Accession Version	XM_021854100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	titin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709792.1
CDS	292..11247
Translation	MDTTSAAAALNPGEGSNFGVVHVKQEPGFSGESSPGPEGSIGTDGGDGARTIRKITVLDPLKLKLLSRQNHNKLLSGLNAAKAKAQPPAPKPPPLAAPSSSASTVTEKETPSEVIATDRTVEAESEPLRPDVEEPPKQTSSTSSAPPKTGAGGGSTLFRRKKVVPVAIARKLAQKEKTKPAPKQCEEKTPPATSPTDASPVQCEPVLPEPVEQVPQQPESAPVEPKPVEPESSQHEAKEPAASSEPNPVEQTETREKPVASPPPLVLVKREPSDLVTRPTAFRRIKPKVAICLNPSRPKKSILKKTTSADKLLPAGSNEPSKSFKDLIPEETIVLDDDDEEDNEQHTPSVTVEEADHTGKSEVQRTQQEEAERMAEEETRRIAEEQRQKDAEDQRRIEEGARRKAEEQRRILAEQKKREEEEKRRIAEDQRRAEEEAKKVAEEQCRIQEEMKKREEEQRQKLEQLRIQEEQRKVAEEQKRLEMLEQEEKAKRVEPEKPAAESRKTMKIPPEIWKRQMERMKNNPNRQHSTVPAIPQSNAEPSQSQHTITSDPVNVKTSNVVEQEPVSVVAPAVNQERANQISEKPAVQVTPNPTKEIRRISRLPPSLAMKLMNCVALKPSRNKIEEKAAPSVKPSQNKTETSHATKEVPSSAPAVVSQTAIRRISGLSPSASMTPIKTAEAMQQEVQETTADKQQQSIVPESAVANVERTEKTEPVERTVISNKPAPTKEIKRIALPPDIALKLLGAVSRNKPVVVTSTAGTVDPAKNVASKPATIAEKPAVAEPESSNPTVTAAAPIVVPPIKQTTIQSGTNAPKEIRRISQLPPNVAMRLVDSLKQKGITVPKLCLKKDTPTIEPVSVAASDVQLPSEPSTPSSVASVPEIKVEPQEQPTKSKGKVEILSQVILPTASYNLEELLAGKSTEGFAPSCAMPLPLPMHKGRRQAAPVPIAPVPTSIHSEPIQHSQVSLPVQQPSKPETPAPVTAVPSPIPDLPVAMAENDIASEVTVTTENEDIPNPSHEKFDEYIISYSDCEGAPVQYDPSKSAQQQNKEEIPPEPNVTFVSSNAPTVMSHQYSVTYSANSHQQLHRPNVQAPIVIQQPANVSSYQPRPVAYFTKVSNNTQRPSPQPTPVTYHPQFTVLSQPGMNPTRMISQRMIINPAASMQSGQRPRYPTAQFVVLRHPTPIRGQLVAQTQLRQEHPQPVPQQSQQHLTVPRETYYHRLPIVNQNQPSVIVPTPRYVIPKQYPQIQMARPPRPQRPSVDIAQNQSKAFQPVQQSQPTTAFSIAQPVQQQRVIPKIVIDSSYVDQQERGTPASSNTPPLQDQWKTQNTAPCSEEIRNKLRDKLKSRMLQQSAKSGSPTVETPPPNPLSTPSSAAPKASPAKTPVGPPTIPVRNTVVYHPQDFQQRIRSQQAQPPKPVDTRIIKQYICQGRKIYLTKISVTKPTQASSSNVQQTEPPQQFLQYRQPQTDTGFHGFPQAAIKCDQRNFQQFNETVQQLERRKVPEPSDESGMIEIQNIQKIRAKMRNRKPSVVTAVTTHGLKGETFIITELNDPSLHPMEMHSPEVKQEVPLQHPPRITRRRNTFVVDDSDGDVQTDQLPQVEMTSQDDSQQDDKPSEMELKVEVENEEKEPVTNAERLAFDRCMKEEVPSGSESSQADEDLAREILALVENERKALRAGPTVKKVKKRRKRRKRHKKKSDGNEELMDLLMKHVNQESLIADIVASQQKKLPVIELSDTDENDEDRTVQRRVTCKDVRVKPDPDAETIGDRKTSCTSDFEESETNDIFAEILKPFVEEEEHAVGSERVVVEETPQKVVSGKPMAESDKACAKEIAGSDEILSTLVEKEKQAVSLKSVATQESLQNAVPWESEDACPEKPSAPAQKATSDDILKSIVEEVKQSNEAEAVPEKPAQKSDEACREAPRVLADEVISIPAVEEIVPENQVTSEEQDVVPEQQAVTLNEMVTDEQRETIPEEKALLPVEAAPAEQIVHVPKQAETSEISSKLVTEVRPEIESNQPTICDQTCDNSALPVKELPKKAPAQRAPKRKLKPTLVSRSKIRKVEPPAAIVTKIQPQPTRVEKSTEVNIEDSSDPFSDRDKPPLPDSEEERIQSQMDTINSRVRELVNEWDSDEEETSIKQPLQKYASFDNNGNLPCRISKNNALKRAAILSDPVLEVSVEESRKEESHEAIEDTSVQATANIVKPVRVTVLKVDLIERSEIVQEPVEPATREKTPPPPTEAKGQTVANLLKDWDESPIKQKKENAPVVARVLLDRLSSSELNQLKSKKAPEVSESSKITMEPSNQSPTIESKAKDSRDEISGDESERVVARIFMGGLDSSGSSGQQQIVPPASQMAAQSSPVSTGSPSKSRKSENKIPNTAESPTTRSKSSKQTSIKDWIQGSPTTEPSKPITGRRKGLRGRAASVLFEPEANQVTEKQKPAGRNLRSRTKSVFAEKPPLMEEEEPVQQEDISRPVEEPSEEQIEVTPEKPSGSRPEVIKEAGGTACAARKEQESKEYSTSTKPTPIIRNRVPIAISSEDEDDAPLAKRRKVLRKRRSMMRRPSAIRDLNANKRLFRGGATLNNSLIVDDSNMGETTFIKSEPPEEIEDIQDYYASPSESFAVTFPDNPSEQLLSVVTVQNNQVVHSQDFDAGSPFRNQYFAKDLIQSAATSTPNAQSPIQPPPPVQDHVFATPEPVRAAKRKRRTKLEMENDRRLEAMKREEEQKPEELKKKEAQRGRVFKSFTPVKVDAVDPNISQFECASCFEHVSRDDWDAHYAKHSGLTYRVNVDPVVDLNDETTAVAIVTRFMKAHRQVTLYCDKCNVAKKSALGLVSHRPVCGLTRQEILNTKIPCQHCGRKLMPVSMNCHLLGHCAVLKRKKQEEALETKLEGASADVEMEPTTLNQRGTVKRQATAKAERRIQNISFSEMILSVTKDKVTEGCLAAWKTQFRKAKVALCVFRTCSFTGTDEIEMRHHHEFCPSPCELLECKNCKFQTEKIDQMVKHIKLKHNDVLVCMTIDDSSGTDAKMTSESSDDEGGTGVDENDEPYMIGIDDEDKPKKRKIKTLLKTCKLSTRDTFNEESDVYREMILDEVINLRLLKSEFHLTAREWTAEFRRLHYSTSILFSALRPDLDVRFLTFNMTQEYIPKQTHSLRFVARSTNLYNAPIQLKEYANKWQNLEVFTGQVNEKDTIFFCGGPVVALDWLPIPDGQHEHQIVAVACKNDFEEFYLADRIIPVKCLIQIWDVGLLDNKEIYNKKAENAKPTLLYSIACDVGPIWALKFCPSGCYNSKTSGDDYDRLGLLAAAGSDGNIHIFSLGRSYEHLISNSSRVINLAPVLKLTLSLADQSCCSSYESHSAVKLAWSKSKHHSMLAAGYSNGTVAVWNATSTSPLLTGTKDNVRALLPVHKVFIPDSCVTAVDLHYSEDSRYLLVCNADRKVVVYDLNTGYMPMEICSLNARSKVTTACWNIHFPLITMAFDDVYAIDRCALTFHQPREIGVRLHPLYTHTAEATDMSNSDHLSTHVIGTDGGDVLSHKPMAFIHNMGQKNPAQIKYVLTSTISVKVNEEQMDNSYNKFEKNYCVLFSDFDKNPARMDLKSLQVKTFRRALLHEYPGIRINQVKWNPNDKSHQYYAIGYQAGFVRVRPFRLK

Target ORF information:

RefSeq Version	XM_021854100.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti titin (LOC5568954), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021854100.1

ORF Insert Sequence:

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568954 ( XM_021854095.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709787.1 Aedes aegypti titin (LOC5568954), transcript variant X1, mRNA.

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CloneID	OAe129075
Clone ID Related Accession (Same CDS sequence)	XM_021854098.1 , XM_021854096.1 , XM_021854097.1 , XM_021854099.1 , XM_021854100.1 , XM_021854095.1
Accession Version	XM_021854095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	titin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709787.1
CDS	418..11373
Translation	MDTTSAAAALNPGEGSNFGVVHVKQEPGFSGESSPGPEGSIGTDGGDGARTIRKITVLDPLKLKLLSRQNHNKLLSGLNAAKAKAQPPAPKPPPLAAPSSSASTVTEKETPSEVIATDRTVEAESEPLRPDVEEPPKQTSSTSSAPPKTGAGGGSTLFRRKKVVPVAIARKLAQKEKTKPAPKQCEEKTPPATSPTDASPVQCEPVLPEPVEQVPQQPESAPVEPKPVEPESSQHEAKEPAASSEPNPVEQTETREKPVASPPPLVLVKREPSDLVTRPTAFRRIKPKVAICLNPSRPKKSILKKTTSADKLLPAGSNEPSKSFKDLIPEETIVLDDDDEEDNEQHTPSVTVEEADHTGKSEVQRTQQEEAERMAEEETRRIAEEQRQKDAEDQRRIEEGARRKAEEQRRILAEQKKREEEEKRRIAEDQRRAEEEAKKVAEEQCRIQEEMKKREEEQRQKLEQLRIQEEQRKVAEEQKRLEMLEQEEKAKRVEPEKPAAESRKTMKIPPEIWKRQMERMKNNPNRQHSTVPAIPQSNAEPSQSQHTITSDPVNVKTSNVVEQEPVSVVAPAVNQERANQISEKPAVQVTPNPTKEIRRISRLPPSLAMKLMNCVALKPSRNKIEEKAAPSVKPSQNKTETSHATKEVPSSAPAVVSQTAIRRISGLSPSASMTPIKTAEAMQQEVQETTADKQQQSIVPESAVANVERTEKTEPVERTVISNKPAPTKEIKRIALPPDIALKLLGAVSRNKPVVVTSTAGTVDPAKNVASKPATIAEKPAVAEPESSNPTVTAAAPIVVPPIKQTTIQSGTNAPKEIRRISQLPPNVAMRLVDSLKQKGITVPKLCLKKDTPTIEPVSVAASDVQLPSEPSTPSSVASVPEIKVEPQEQPTKSKGKVEILSQVILPTASYNLEELLAGKSTEGFAPSCAMPLPLPMHKGRRQAAPVPIAPVPTSIHSEPIQHSQVSLPVQQPSKPETPAPVTAVPSPIPDLPVAMAENDIASEVTVTTENEDIPNPSHEKFDEYIISYSDCEGAPVQYDPSKSAQQQNKEEIPPEPNVTFVSSNAPTVMSHQYSVTYSANSHQQLHRPNVQAPIVIQQPANVSSYQPRPVAYFTKVSNNTQRPSPQPTPVTYHPQFTVLSQPGMNPTRMISQRMIINPAASMQSGQRPRYPTAQFVVLRHPTPIRGQLVAQTQLRQEHPQPVPQQSQQHLTVPRETYYHRLPIVNQNQPSVIVPTPRYVIPKQYPQIQMARPPRPQRPSVDIAQNQSKAFQPVQQSQPTTAFSIAQPVQQQRVIPKIVIDSSYVDQQERGTPASSNTPPLQDQWKTQNTAPCSEEIRNKLRDKLKSRMLQQSAKSGSPTVETPPPNPLSTPSSAAPKASPAKTPVGPPTIPVRNTVVYHPQDFQQRIRSQQAQPPKPVDTRIIKQYICQGRKIYLTKISVTKPTQASSSNVQQTEPPQQFLQYRQPQTDTGFHGFPQAAIKCDQRNFQQFNETVQQLERRKVPEPSDESGMIEIQNIQKIRAKMRNRKPSVVTAVTTHGLKGETFIITELNDPSLHPMEMHSPEVKQEVPLQHPPRITRRRNTFVVDDSDGDVQTDQLPQVEMTSQDDSQQDDKPSEMELKVEVENEEKEPVTNAERLAFDRCMKEEVPSGSESSQADEDLAREILALVENERKALRAGPTVKKVKKRRKRRKRHKKKSDGNEELMDLLMKHVNQESLIADIVASQQKKLPVIELSDTDENDEDRTVQRRVTCKDVRVKPDPDAETIGDRKTSCTSDFEESETNDIFAEILKPFVEEEEHAVGSERVVVEETPQKVVSGKPMAESDKACAKEIAGSDEILSTLVEKEKQAVSLKSVATQESLQNAVPWESEDACPEKPSAPAQKATSDDILKSIVEEVKQSNEAEAVPEKPAQKSDEACREAPRVLADEVISIPAVEEIVPENQVTSEEQDVVPEQQAVTLNEMVTDEQRETIPEEKALLPVEAAPAEQIVHVPKQAETSEISSKLVTEVRPEIESNQPTICDQTCDNSALPVKELPKKAPAQRAPKRKLKPTLVSRSKIRKVEPPAAIVTKIQPQPTRVEKSTEVNIEDSSDPFSDRDKPPLPDSEEERIQSQMDTINSRVRELVNEWDSDEEETSIKQPLQKYASFDNNGNLPCRISKNNALKRAAILSDPVLEVSVEESRKEESHEAIEDTSVQATANIVKPVRVTVLKVDLIERSEIVQEPVEPATREKTPPPPTEAKGQTVANLLKDWDESPIKQKKENAPVVARVLLDRLSSSELNQLKSKKAPEVSESSKITMEPSNQSPTIESKAKDSRDEISGDESERVVARIFMGGLDSSGSSGQQQIVPPASQMAAQSSPVSTGSPSKSRKSENKIPNTAESPTTRSKSSKQTSIKDWIQGSPTTEPSKPITGRRKGLRGRAASVLFEPEANQVTEKQKPAGRNLRSRTKSVFAEKPPLMEEEEPVQQEDISRPVEEPSEEQIEVTPEKPSGSRPEVIKEAGGTACAARKEQESKEYSTSTKPTPIIRNRVPIAISSEDEDDAPLAKRRKVLRKRRSMMRRPSAIRDLNANKRLFRGGATLNNSLIVDDSNMGETTFIKSEPPEEIEDIQDYYASPSESFAVTFPDNPSEQLLSVVTVQNNQVVHSQDFDAGSPFRNQYFAKDLIQSAATSTPNAQSPIQPPPPVQDHVFATPEPVRAAKRKRRTKLEMENDRRLEAMKREEEQKPEELKKKEAQRGRVFKSFTPVKVDAVDPNISQFECASCFEHVSRDDWDAHYAKHSGLTYRVNVDPVVDLNDETTAVAIVTRFMKAHRQVTLYCDKCNVAKKSALGLVSHRPVCGLTRQEILNTKIPCQHCGRKLMPVSMNCHLLGHCAVLKRKKQEEALETKLEGASADVEMEPTTLNQRGTVKRQATAKAERRIQNISFSEMILSVTKDKVTEGCLAAWKTQFRKAKVALCVFRTCSFTGTDEIEMRHHHEFCPSPCELLECKNCKFQTEKIDQMVKHIKLKHNDVLVCMTIDDSSGTDAKMTSESSDDEGGTGVDENDEPYMIGIDDEDKPKKRKIKTLLKTCKLSTRDTFNEESDVYREMILDEVINLRLLKSEFHLTAREWTAEFRRLHYSTSILFSALRPDLDVRFLTFNMTQEYIPKQTHSLRFVARSTNLYNAPIQLKEYANKWQNLEVFTGQVNEKDTIFFCGGPVVALDWLPIPDGQHEHQIVAVACKNDFEEFYLADRIIPVKCLIQIWDVGLLDNKEIYNKKAENAKPTLLYSIACDVGPIWALKFCPSGCYNSKTSGDDYDRLGLLAAAGSDGNIHIFSLGRSYEHLISNSSRVINLAPVLKLTLSLADQSCCSSYESHSAVKLAWSKSKHHSMLAAGYSNGTVAVWNATSTSPLLTGTKDNVRALLPVHKVFIPDSCVTAVDLHYSEDSRYLLVCNADRKVVVYDLNTGYMPMEICSLNARSKVTTACWNIHFPLITMAFDDVYAIDRCALTFHQPREIGVRLHPLYTHTAEATDMSNSDHLSTHVIGTDGGDVLSHKPMAFIHNMGQKNPAQIKYVLTSTISVKVNEEQMDNSYNKFEKNYCVLFSDFDKNPARMDLKSLQVKTFRRALLHEYPGIRINQVKWNPNDKSHQYYAIGYQAGFVRVRPFRLK

Target ORF information:

RefSeq Version	XM_021854095.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti titin (LOC5568954), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021854095.1

ORF Insert Sequence:

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGGT ATCCAACAAT 
ACACAACGAC CTTCCCCTCA ACCAACACCC GTAACATATC ATCCACAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568954 ( XM_021854101.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709793.1 Aedes aegypti titin (LOC5568954), transcript variant X7, mRNA.

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CloneID	OAe129076
Clone ID Related Accession (Same CDS sequence)	XM_021854101.1
Accession Version	XM_021854101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	titin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709793.1
CDS	418..11313
Translation	MDTTSAAAALNPGEGSNFGVVHVKQEPGFSGESSPGPEGSIGTDGGDGARTIRKITVLDPLKLKLLSRQNHNKLLSGLNAAKAKAQPPAPKPPPLAAPSSSASTVTEKETPSEVIATDRTVEAESEPLRPDVEEPPKQTSSTSSAPPKTGAGGGSTLFRRKKVVPVAIARKLAQKEKTKPAPKQCEEKTPPATSPTDASPVQCEPVLPEPVEQVPQQPESAPVEPKPVEPESSQHEAKEPAASSEPNPVEQTETREKPVASPPPLVLVKREPSDLVTRPTAFRRIKPKVAICLNPSRPKKSILKKTTSADKLLPAGSNEPSKSFKDLIPEETIVLDDDDEEDNEQHTPSVTVEEADHTGKSEVQRTQQEEAERMAEEETRRIAEEQRQKDAEDQRRIEEGARRKAEEQRRILAEQKKREEEEKRRIAEDQRRAEEEAKKVAEEQCRIQEEMKKREEEQRQKLEQLRIQEEQRKVAEEQKRLEMLEQEEKAKRVEPEKPAAESRKTMKIPPEIWKRQMERMKNNPNRQHSTVPAIPQSNAEPSQSQHTITSDPVNVKTSNVVEQEPVSVVAPAVNQERANQISEKPAVQVTPNPTKEIRRISRLPPSLAMKLMNCVALKPSRNKIEEKAAPSVKPSQNKTETSHATKEVPSSAPAVVSQTAIRRISGLSPSASMTPIKTAEAMQQEVQETTADKQQQSIVPESAVANVERTEKTEPVERTVISNKPAPTKEIKRIALPPDIALKLLGAVSRNKPVVVTSTAGTVDPAKNVASKPATIAEKPAVAEPESSNPTVTAAAPIVVPPIKQTTIQSGTNAPKEIRRISQLPPNVAMRLVDSLKQKGITVPKLCLKKDTPTIEPVSVAASDVQLPSEPSTPSSVASVPEIKVEPQEQPTKSKGKVEILSQVILPTASYNLEELLAGKSTEGFAPSCAMPLPLPMHKGRRQAAPVPIAPVPTSIHSEPIQHSQVSLPVQQPSKPETPAPVTAVPSPIPDLPVAMAENDIASEVTVTTENEDIPNPSHEKFDEYIISYSDCEGAPVQYDPSKSAQQQNKEEIPPEPNVTFVSSNAPTVMSHQYSVTYSANSHQQLHRPNVQAPIVIQQPANVSSYQPRPVAYFTKFTVLSQPGMNPTRMISQRMIINPAASMQSGQRPRYPTAQFVVLRHPTPIRGQLVAQTQLRQEHPQPVPQQSQQHLTVPRETYYHRLPIVNQNQPSVIVPTPRYVIPKQYPQIQMARPPRPQRPSVDIAQNQSKAFQPVQQSQPTTAFSIAQPVQQQRVIPKIVIDSSYVDQQERGTPASSNTPPLQDQWKTQNTAPCSEEIRNKLRDKLKSRMLQQSAKSGSPTVETPPPNPLSTPSSAAPKASPAKTPVGPPTIPVRNTVVYHPQDFQQRIRSQQAQPPKPVDTRIIKQYICQGRKIYLTKISVTKPTQASSSNVQQTEPPQQFLQYRQPQTDTGFHGFPQAAIKCDQRNFQQFNETVQQLERRKVPEPSDESGMIEIQNIQKIRAKMRNRKPSVVTAVTTHGLKGETFIITELNDPSLHPMEMHSPEVKQEVPLQHPPRITRRRNTFVVDDSDGDVQTDQLPQVEMTSQDDSQQDDKPSEMELKVEVENEEKEPVTNAERLAFDRCMKEEVPSGSESSQADEDLAREILALVENERKALRAGPTVKKVKKRRKRRKRHKKKSDGNEELMDLLMKHVNQESLIADIVASQQKKLPVIELSDTDENDEDRTVQRRVTCKDVRVKPDPDAETIGDRKTSCTSDFEESETNDIFAEILKPFVEEEEHAVGSERVVVEETPQKVVSGKPMAESDKACAKEIAGSDEILSTLVEKEKQAVSLKSVATQESLQNAVPWESEDACPEKPSAPAQKATSDDILKSIVEEVKQSNEAEAVPEKPAQKSDEACREAPRVLADEVISIPAVEEIVPENQVTSEEQDVVPEQQAVTLNEMVTDEQRETIPEEKALLPVEAAPAEQIVHVPKQAETSEISSKLVTEVRPEIESNQPTICDQTCDNSALPVKELPKKAPAQRAPKRKLKPTLVSRSKIRKVEPPAAIVTKIQPQPTRVEKSTEVNIEDSSDPFSDRDKPPLPDSEEERIQSQMDTINSRVRELVNEWDSDEEETSIKQPLQKYASFDNNGNLPCRISKNNALKRAAILSDPVLEVSVEESRKEESHEAIEDTSVQATANIVKPVRVTVLKVDLIERSEIVQEPVEPATREKTPPPPTEAKGQTVANLLKDWDESPIKQKKENAPVVARVLLDRLSSSELNQLKSKKAPEVSESSKITMEPSNQSPTIESKAKDSRDEISGDESERVVARIFMGGLDSSGSSGQQQIVPPASQMAAQSSPVSTGSPSKSRKSENKIPNTAESPTTRSKSSKQTSIKDWIQGSPTTEPSKPITGRRKGLRGRAASVLFEPEANQVTEKQKPAGRNLRSRTKSVFAEKPPLMEEEEPVQQEDISRPVEEPSEEQIEVTPEKPSGSRPEVIKEAGGTACAARKEQESKEYSTSTKPTPIIRNRVPIAISSEDEDDAPLAKRRKVLRKRRSMMRRPSAIRDLNANKRLFRGGATLNNSLIVDDSNMGETTFIKSEPPEEIEDIQDYYASPSESFAVTFPDNPSEQLLSVVTVQNNQVVHSQDFDAGSPFRNQYFAKDLIQSAATSTPNAQSPIQPPPPVQDHVFATPEPVRAAKRKRRTKLEMENDRRLEAMKREEEQKPEELKKKEAQRGRVFKSFTPVKVDAVDPNISQFECASCFEHVSRDDWDAHYAKHSGLTYRVNVDPVVDLNDETTAVAIVTRFMKAHRQVTLYCDKCNVAKKSALGLVSHRPVCGLTRQEILNTKIPCQHCGRKLMPVSMNCHLLGHCAVLKRKKQEEALETKLEGASADVEMEPTTLNQRGTVKRQATAKAERRIQNISFSEMILSVTKDKVTEGCLAAWKTQFRKAKVALCVFRTCSFTGTDEIEMRHHHEFCPSPCELLECKNCKFQTEKIDQMVKHIKLKHNDVLVCMTIDDSSGTDAKMTSESSDDEGGTGVDENDEPYMIGIDDEDKPKKRKIKTLLKTCKLSTRDTFNEESDVYREMILDEVINLRLLKSEFHLTAREWTAEFRRLHYSTSILFSALRPDLDVRFLTFNMTQEYIPKQTHSLRFVARSTNLYNAPIQLKEYANKWQNLEVFTGQVNEKDTIFFCGGPVVALDWLPIPDGQHEHQIVAVACKNDFEEFYLADRIIPVKCLIQIWDVGLLDNKEIYNKKAENAKPTLLYSIACDVGPIWALKFCPSGCYNSKTSGDDYDRLGLLAAAGSDGNIHIFSLGRSYEHLISNSSRVINLAPVLKLTLSLADQSCCSSYESHSAVKLAWSKSKHHSMLAAGYSNGTVAVWNATSTSPLLTGTKDNVRALLPVHKVFIPDSCVTAVDLHYSEDSRYLLVCNADRKVVVYDLNTGYMPMEICSLNARSKVTTACWNIHFPLITMAFDDVYAIDRCALTFHQPREIGVRLHPLYTHTAEATDMSNSDHLSTHVIGTDGGDVLSHKPMAFIHNMGQKNPAQIKYVLTSTISVKVNEEQMDNSYNKFEKNYCVLFSDFDKNPARMDLKSLQVKTFRRALLHEYPGIRINQVKWNPNDKSHQYYAIGYQAGFVRVRPFRLK

Target ORF information:

RefSeq Version	XM_021854101.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti titin (LOC5568954), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021854101.1

ORF Insert Sequence:

ATGGATACTA CGTCAGCAGC AGCAGCTCTA AATCCAGGAG AAGGATCGAA CTTTGGTGTG 
GTTCACGTCA AACAGGAGCC CGGATTCAGT GGCGAATCTT CCCCAGGGCC CGAGGGATCG 
ATAGGCACCG ATGGCGGCGA TGGGGCGCGA ACCATCCGGA AAATTACGGT GCTGGATCCG 
TTGAAGCTGA AGCTGCTCAG CCGACAGAAC CATAACAAGT TGCTGTCCGG GTTGAATGCA 
GCCAAAGCAA AAGCGCAACC TCCTGCTCCT AAACCACCAC CTCTAGCAGC ACCGTCGTCA 
AGTGCATCAA CAGTGACCGA AAAAGAAACT CCGTCAGAAG TGATAGCCAC CGACAGGACT 
GTAGAAGCAG AATCGGAACC GTTACGACCC GATGTTGAGG AACCTCCAAA GCAAACATCT 
TCAACTTCGT CGGCACCACC CAAAACAGGG GCAGGAGGAG GCTCTACACT GTTCAGGAGG 
AAGAAGGTAG TACCAGTGGC CATAGCACGT AAGCTAGCTC AAAAAGAAAA GACTAAACCC 
GCACCAAAAC AGTGTGAAGA AAAAACTCCC CCGGCGACAT CACCTACGGA TGCATCACCA 
GTTCAATGTG AACCTGTTCT ACCAGAGCCG GTTGAGCAGG TACCTCAACA ACCTGAATCT 
GCTCCTGTAG AACCCAAACC GGTCGAACCT GAATCTTCTC AACACGAAGC AAAAGAACCA 
GCAGCGTCTT CAGAACCCAA CCCGGTGGAG CAAACCGAGA CCAGAGAGAA ACCAGTGGCT 
AGCCCACCAC CTCTCGTTCT GGTGAAACGT GAACCGTCAG ATCTGGTGAC ACGTCCGACG 
GCATTCCGCA GAATCAAACC AAAGGTTGCA ATCTGTCTCA ATCCAAGCCG TCCGAAGAAG 
TCAATTCTCA AAAAGACCAC TTCTGCGGAC AAATTGCTCC CGGCGGGATC GAACGAGCCG 
AGCAAATCTT TCAAGGATTT GATTCCAGAG GAGACGATTG TGCTGGACGA TGATGATGAG 
GAAGACAACG AGCAGCACAC GCCAAGCGTT ACGGTTGAAG AGGCTGATCA TACAGGAAAG 
TCGGAGGTCC AGCGAACGCA GCAAGAGGAA GCAGAACGTA TGGCTGAGGA GGAAACGCGA 
CGAATAGCGG AGGAGCAACG TCAAAAGGAT GCTGAAGATC AGAGAAGGAT AGAGGAAGGG 
GCTAGGAGAA AGGCTGAAGA ACAGCGTAGG ATATTAGCGG AACAGAAGAA ACGAGAAGAG 
GAAGAAAAGC GACGAATAGC GGAAGATCAA CGGAGAGCTG AAGAAGAGGC TAAAAAAGTG 
GCTGAAGAAC AGTGCAGAAT ACAAGAAGAA ATGAAGAAAC GAGAAGAAGA ACAGCGACAG 
AAGCTAGAAC AACTACGAAT ACAAGAAGAA CAGCGGAAAG TTGCAGAGGA ACAAAAGCGG 
TTGGAAATGC TCGAGCAGGA GGAGAAAGCC AAACGTGTGG AACCAGAGAA ACCCGCAGCT 
GAAAGTAGGA AAACCATGAA AATACCGCCG GAAATTTGGA AACGACAAAT GGAGAGGATG 
AAAAACAACC CGAACCGACA GCATAGTACG GTGCCGGCAA TTCCACAGTC GAATGCGGAA 
CCATCGCAAT CTCAACACAC GATCACATCG GATCCTGTTA ATGTTAAAAC ATCCAATGTG 
GTCGAGCAGG AGCCTGTTAG TGTTGTAGCT CCTGCTGTAA ATCAAGAAAG AGCTAACCAA 
ATCAGTGAGA AGCCTGCTGT GCAGGTGACT CCAAATCCTA CTAAAGAAAT CCGACGAATA 
TCTCGGCTTC CTCCCAGTTT GGCAATGAAG CTGATGAACT GTGTCGCTTT GAAGCCTTCC 
AGGAACAAAA TAGAGGAGAA GGCTGCACCG TCTGTAAAAC CCTCACAGAA TAAAACAGAA 
ACATCGCATG CAACGAAAGA AGTACCATCA TCGGCACCAG CAGTAGTTTC ACAAACTGCA 
ATTAGGCGAA TATCCGGGCT TTCACCGAGT GCGAGTATGA CGCCAATAAA AACTGCTGAA 
GCGATGCAAC AGGAAGTCCA GGAAACGACA GCCGATAAAC AGCAGCAGTC AATTGTACCT 
GAGAGCGCGG TCGCAAATGT TGAGAGAACC GAAAAGACTG AACCGGTGGA GCGTACTGTG 
ATATCTAACA AGCCGGCGCC GACGAAAGAG ATTAAACGGA TAGCACTTCC GCCAGATATT 
GCTTTGAAGT TGTTAGGAGC AGTTAGTAGA AATAAACCGG TGGTTGTGAC ATCCACGGCC 
GGAACAGTTG ATCCAGCGAA AAATGTTGCA TCTAAACCTG CAACGATCGC TGAAAAGCCT 
GCGGTCGCTG AACCCGAGTC GTCGAACCCT ACTGTAACGG CCGCTGCTCC CATCGTTGTT 
CCTCCAATAA AGCAAACAAC AATTCAGAGT GGTACAAATG CTCCGAAAGA AATCCGTCGA 
ATATCACAAC TGCCTCCCAA TGTCGCGATG AGGCTGGTGG ATTCTTTGAA ACAGAAAGGA 
ATAACTGTAC CAAAGTTGTG TCTTAAGAAG GATACTCCTA CCATCGAACC AGTTAGTGTA 
GCTGCATCAG ATGTACAACT TCCCAGTGAA CCATCGACAC CGTCTTCCGT GGCATCGGTG 
CCGGAAATTA AGGTGGAACC ACAGGAGCAG CCAACCAAAT CCAAAGGAAA GGTGGAAATC 
TTGTCACAGG TTATTTTGCC TACGGCATCC TACAATCTGG AGGAGCTATT GGCTGGCAAA 
TCAACTGAAG GCTTTGCGCC ATCCTGTGCG ATGCCTCTTC CGTTACCCAT GCACAAAGGA 
AGAAGGCAAG CGGCTCCTGT CCCTATTGCG CCAGTTCCTA CATCAATTCA TAGTGAGCCG 
ATACAGCATA GTCAAGTCTC TTTACCTGTA CAACAGCCTA GCAAACCAGA GACTCCTGCA 
CCGGTTACAG CGGTACCATC GCCGATTCCA GATCTTCCGG TTGCAATGGC AGAAAATGAT 
ATCGCTTCTG AAGTCACTGT CACAACGGAG AATGAAGATA TCCCAAATCC ATCGCATGAA 
AAATTCGACG AATACATTAT TTCCTATTCA GATTGTGAGG GAGCTCCAGT GCAATATGAT 
CCATCAAAAA GCGCCCAACA GCAAAATAAG GAAGAAATCC CACCTGAGCC GAATGTTACT 
TTTGTTTCCT CAAACGCTCC TACTGTTATG TCGCATCAAT ATTCCGTTAC GTACTCAGCG 
AATTCACACC AGCAACTTCA TCGGCCAAAC GTTCAAGCTC CGATAGTAAT TCAACAACCA 
GCAAACGTTT CCAGTTATCA GCCACGCCCC GTCGCCTATT TCACCAAGTT TACGGTGTTA 
TCACAGCCCG GAATGAACCC TACCCGCATG ATATCCCAGC GAATGATCAT CAATCCAGCA 
GCCAGCATGC AAAGTGGCCA AAGACCTCGG TATCCAACAG CTCAGTTTGT TGTGCTACGC 
CACCCCACTC CAATCAGAGG ACAGTTAGTT GCTCAAACTC AACTTCGGCA GGAACATCCA 
CAGCCTGTGC CGCAACAATC GCAACAGCAT CTAACTGTCC CTAGAGAAAC CTATTATCAT 
CGCTTGCCAA TAGTCAACCA GAATCAGCCT TCAGTTATTG TACCAACGCC GCGATACGTT 
ATCCCCAAAC AGTACCCTCA AATTCAAATG GCTCGTCCTC CGCGTCCGCA GCGTCCTTCC 
GTAGACATCG CCCAAAATCA AAGCAAAGCA TTCCAACCTG TTCAACAATC GCAACCGACC 
ACCGCATTCT CCATCGCTCA ACCTGTACAA CAGCAACGTG TAATTCCGAA GATCGTCATC 
GACAGCAGCT ACGTGGATCA GCAAGAACGA GGGACTCCAG CTTCTTCAAA CACTCCACCT 
CTTCAAGATC AATGGAAAAC ACAGAATACG GCACCCTGTT CGGAAGAGAT CAGGAACAAA 
CTCCGGGACA AATTGAAGTC TCGAATGCTG CAACAAAGTG CCAAATCAGG AAGCCCAACT 
GTGGAAACCC CACCGCCAAA CCCACTATCA ACGCCTTCAT CAGCGGCTCC CAAAGCCTCA 
CCAGCAAAAA CTCCGGTTGG ACCTCCTACA ATACCCGTTC GTAACACCGT AGTTTACCAT 
CCGCAAGACT TCCAGCAACG CATTCGTTCA CAACAAGCGC AACCACCCAA ACCGGTAGAT 
ACTCGCATCA TCAAGCAGTA CATATGTCAG GGAAGGAAAA TTTACCTCAC CAAAATCAGT 
GTCACAAAAC CCACACAGGC CAGTTCCTCG AACGTTCAGC AGACAGAGCC ACCTCAGCAG 
TTCCTACAGT ATCGGCAGCC CCAAACGGAC ACGGGCTTTC ATGGTTTCCC ACAGGCAGCG 
ATAAAATGCG ATCAACGCAA TTTCCAGCAG TTCAACGAAA CGGTTCAGCA GTTGGAGCGG 
AGGAAAGTTC CAGAACCTTC GGATGAAAGC GGGATGATTG AAATTCAGAA TATCCAGAAA 
ATTCGTGCCA AGATGCGAAA TCGGAAACCG AGTGTGGTGA CGGCTGTAAC AACACACGGC 
TTGAAAGGAG AAACTTTCAT CATTACGGAA CTGAACGATC CGTCGCTGCA TCCAATGGAG 
ATGCATTCAC CGGAAGTGAA GCAAGAAGTT CCGCTACAAC ATCCTCCTAG AATCACCAGA 
CGAAGAAATA CCTTTGTAGT GGATGATTCG GATGGAGACG TTCAAACCGA TCAGTTACCA 
CAGGTGGAAA TGACTAGTCA GGATGATAGC CAACAGGATG ATAAACCATC TGAAATGGAA 
CTCAAGGTGG AAGTCGAGAA TGAGGAAAAG GAACCGGTAA CGAACGCGGA ACGATTAGCA 
TTCGATCGGT GTATGAAGGA AGAGGTACCA TCTGGATCCG AATCCAGCCA GGCTGACGAA 
GATCTGGCAC GGGAGATTTT GGCTCTGGTG GAAAACGAAC GCAAGGCTTT GCGCGCTGGG 
CCAACGGTGA AGAAAGTCAA GAAGCGAAGA AAGCGACGTA AAAGGCACAA GAAGAAAAGT 
GATGGCAATG AGGAGTTGAT GGATCTCTTG ATGAAGCATG TCAATCAGGA GTCGTTAATT 
GCGGATATCG TGGCCTCGCA GCAGAAGAAA TTGCCTGTCA TTGAGCTATC GGATACGGAT 
GAGAATGATG AGGATAGAAC AGTGCAGAGG AGAGTGACCT GCAAGGATGT TCGAGTGAAG 
CCAGATCCTG ATGCGGAAAC AATCGGCGAT AGGAAAACAT CGTGTACGAG TGACTTCGAG 
GAATCTGAGA CGAATGACAT TTTTGCCGAG ATTTTGAAAC CGTTTGTCGA GGAAGAGGAA 
CATGCGGTTG GATCGGAAAG AGTTGTGGTG GAAGAAACGC CGCAGAAAGT TGTCTCAGGT 
AAACCGATGG CTGAGTCGGA TAAAGCATGT GCAAAGGAAA TAGCTGGTTC AGATGAGATT 
CTAAGTACGC TTGTCGAAAA AGAGAAACAG GCGGTTAGCC TTAAAAGCGT AGCGACTCAA 
GAATCGTTGC AGAATGCTGT TCCATGGGAA TCAGAGGATG CATGTCCAGA GAAGCCGTCA 
GCACCAGCGC AGAAAGCTAC TTCAGATGAC ATTTTGAAAT CGATTGTCGA GGAAGTGAAA 
CAGTCGAATG AAGCGGAAGC TGTTCCTGAG AAACCGGCAC AAAAATCGGA TGAAGCATGT 
CGAGAAGCGC CGAGAGTACT GGCGGATGAA GTTATTTCAA TACCTGCAGT GGAAGAAATC 
GTTCCAGAAA ATCAGGTAAC ATCAGAGGAA CAGGATGTTG TTCCAGAACA GCAAGCAGTA 
ACATTGAATG AGATGGTTAC CGATGAGCAG AGAGAAACGA TACCAGAAGA AAAGGCACTT 
TTACCAGTGG AAGCCGCTCC GGCGGAACAA ATAGTGCATG TGCCAAAACA AGCCGAAACA 
TCTGAGATTT CATCAAAATT AGTTACGGAA GTCAGACCAG AGATTGAATC GAATCAACCT 
ACCATATGTG ATCAAACCTG TGATAACAGT GCCTTACCGG TCAAAGAGCT GCCGAAAAAA 
GCACCTGCTC AAAGAGCACC CAAACGCAAG CTCAAACCAA CGTTAGTTTC TCGATCAAAG 
ATAAGAAAAG TTGAACCTCC AGCAGCGATA GTCACCAAAA TCCAACCACA ACCAACAAGA 
GTCGAAAAGT CAACGGAAGT CAACATTGAA GATTCCAGTG ATCCGTTCTC AGATCGAGAT 
AAGCCGCCAC TACCGGATTC GGAGGAAGAG CGAATCCAGT CTCAAATGGA TACGATCAAC 
TCCAGAGTAC GGGAACTAGT CAATGAGTGG GATTCTGATG AAGAAGAGAC AAGTATAAAG 
CAACCATTGC AGAAATATGC CTCGTTCGAT AACAACGGTA ATCTTCCGTG TCGCATTTCA 
AAAAATAATG CGTTGAAACG TGCTGCGATA CTGAGCGATC CGGTTTTGGA AGTTTCTGTT 
GAGGAAAGCC GCAAGGAGGA ATCACACGAA GCGATTGAGG ATACTTCGGT GCAAGCTACA 
GCCAACATCG TTAAGCCGGT AAGAGTTACC GTCCTAAAGG TGGATTTGAT AGAACGGTCA 
GAGATCGTAC AAGAGCCGGT AGAACCCGCT ACAAGAGAGA AGACTCCGCC GCCACCAACT 
GAAGCGAAGG GTCAAACCGT CGCGAATTTG TTGAAGGACT GGGATGAATC ACCGATCAAA 
CAAAAAAAGG AGAACGCTCC AGTGGTTGCT CGGGTGCTAT TAGATAGACT CAGCTCATCT 
GAGTTGAATC AGTTGAAATC CAAAAAGGCT CCGGAAGTGT CAGAATCAAG TAAGATCACG 
ATGGAACCAT CGAATCAATC ACCAACGATT GAAAGTAAGG CGAAGGATAG TCGAGATGAA 
ATAAGCGGTG ACGAATCGGA GAGAGTCGTC GCTCGTATAT TTATGGGCGG ACTAGACAGC 
TCCGGATCAT CTGGACAGCA ACAAATAGTG CCACCAGCAT CACAGATGGC GGCTCAAAGC 
TCTCCAGTTT CTACTGGAAG TCCAAGCAAA TCTCGTAAAA GTGAAAACAA GATTCCAAAT 
ACGGCCGAAT CTCCTACCAC TCGTTCGAAG TCTTCCAAGC AGACATCTAT CAAAGATTGG 
ATTCAAGGTT CTCCAACTAC TGAGCCTTCG AAGCCCATTA CGGGTAGGAG GAAAGGCTTG 
CGTGGAAGAG CCGCATCTGT TCTATTTGAA CCAGAGGCTA ACCAAGTCAC TGAAAAACAG 
AAACCTGCAG GCAGGAATCT GAGAAGCCGT ACTAAATCCG TTTTTGCCGA AAAGCCACCT 
CTGATGGAAG AAGAAGAACC GGTACAGCAA GAAGATATAT CACGACCAGT GGAGGAACCG 
TCTGAGGAAC AGATTGAAGT AACACCGGAA AAGCCCAGCG GAAGTCGTCC GGAGGTGATA 
AAGGAAGCAG GGGGAACCGC TTGTGCGGCA CGGAAAGAAC AAGAATCGAA AGAATACTCT 
ACTAGCACTA AACCTACGCC GATCATTCGG AACCGTGTTC CGATTGCGAT ATCCTCGGAA 
GACGAAGATG ATGCCCCGTT AGCCAAACGT CGCAAAGTGC TTCGCAAGAG GCGTTCCATG 
ATGCGCCGTC CAAGCGCAAT CCGGGATCTG AATGCTAACA AACGTCTGTT CCGAGGAGGC 
GCAACTCTCA ACAATTCTCT CATCGTAGAT GATTCCAACA TGGGCGAAAC TACCTTCATC 
AAAAGCGAAC CTCCGGAGGA GATAGAAGAT ATTCAAGATT ACTATGCCTC TCCGAGTGAA 
TCCTTTGCTG TCACATTCCC GGATAATCCC TCCGAGCAAC TGCTTTCTGT GGTCACTGTG 
CAAAACAATC AAGTAGTGCA CTCACAAGAC TTCGACGCTG GAAGTCCCTT CCGGAACCAG 
TATTTTGCCA AAGATCTTAT TCAATCGGCA GCGACTTCCA CTCCTAACGC CCAATCGCCG 
ATCCAGCCTC CTCCTCCCGT ACAGGATCAT GTCTTTGCCA CTCCGGAACC CGTCCGTGCG 
GCAAAACGGA AGCGTCGTAC GAAACTGGAA ATGGAAAACG ATCGAAGACT TGAAGCGATG 
AAAAGGGAAG AAGAGCAGAA GCCTGAAGAG CTCAAGAAAA AGGAAGCACA ACGAGGCAGA 
GTGTTTAAAT CCTTTACACC AGTCAAGGTG GATGCCGTTG ATCCGAATAT TAGCCAGTTT 
GAATGCGCTT CGTGTTTCGA GCACGTTTCG CGTGATGATT GGGATGCTCA CTATGCGAAG 
CACAGTGGTC TTACCTACCG AGTGAACGTG GATCCTGTGG TTGACCTGAA CGACGAAACA 
ACGGCCGTGG CAATTGTGAC ACGTTTCATG AAGGCCCACC GGCAGGTGAC GCTCTACTGT 
GACAAGTGCA ATGTGGCCAA GAAATCGGCC CTCGGATTAG TGTCGCATCG GCCTGTTTGT 
GGCCTGACGC GGCAAGAGAT CCTAAACACC AAAATACCGT GCCAGCATTG TGGCCGGAAG 
TTGATGCCCG TCAGTATGAA CTGTCACCTG TTGGGACATT GCGCGGTGCT GAAGCGAAAG 
AAACAAGAAG AAGCTTTGGA GACGAAGCTG GAAGGAGCGT CGGCCGATGT GGAAATGGAA 
CCAACAACGC TCAATCAGCG TGGAACGGTT AAGCGCCAAG CGACCGCCAA GGCCGAGAGG 
CGCATCCAGA ACATATCGTT CAGCGAAATG ATTCTCTCCG TCACGAAGGA CAAGGTTACC 
GAGGGTTGTT TGGCTGCTTG GAAAACACAG TTCCGCAAGG CTAAGGTGGC GCTGTGCGTT 
TTTCGGACGT GTTCTTTCAC TGGGACCGAC GAAATAGAAA TGCGCCACCA TCACGAGTTC 
TGTCCTTCGC CCTGTGAGCT ACTCGAGTGC AAGAATTGCA AGTTTCAAAC GGAAAAGATC 
GACCAAATGG TGAAACATAT CAAGCTGAAA CACAACGACG TGCTGGTTTG TATGACGATC 
GATGATAGCA GCGGAACGGA TGCCAAGATG ACGTCGGAAT CATCGGACGA CGAGGGCGGT 
ACGGGTGTAG ACGAAAACGA TGAACCATAC ATGATTGGCA TTGACGATGA GGACAAGCCG 
AAGAAGCGAA AAATCAAAAC TCTGCTGAAA ACGTGCAAAT TGTCTACCAG GGATACGTTC 
AACGAGGAAA GCGACGTTTA TCGAGAGATG ATCCTAGACG AAGTGATCAA TCTTCGGCTG 
CTCAAGAGTG AGTTCCATCT GACGGCACGC GAATGGACAG CGGAGTTCCG ACGGTTGCAT 
TATTCGACCA GCATCCTGTT TTCTGCGTTA CGACCGGATT TGGACGTCCG GTTTCTTACA 
TTTAACATGA CCCAGGAGTA CATCCCGAAG CAGACGCATT CTTTGCGGTT TGTCGCTCGG 
AGTACGAATC TGTACAACGC TCCCATTCAA CTGAAAGAAT ACGCCAACAA GTGGCAGAAC 
TTGGAGGTTT TTACCGGGCA GGTGAATGAG AAGGACACGA TATTCTTCTG TGGGGGACCC 
GTTGTTGCTC TAGATTGGCT GCCAATACCG GACGGCCAGC ATGAACATCA GATAGTGGCG 
GTGGCTTGCA AGAACGATTT CGAAGAATTC TACCTGGCGG ATCGCATCAT CCCGGTGAAG 
TGTTTGATTC AGATCTGGGA CGTGGGCTTA CTGGACAACA AAGAAATCTA CAACAAAAAA 
GCGGAGAATG CCAAACCCAC CTTACTGTAC TCCATAGCGT GCGACGTTGG TCCGATTTGG 
GCGTTGAAGT TCTGCCCGAG TGGATGTTAC AACTCCAAGA CCTCCGGCGA CGATTACGAT 
CGGTTGGGCT TGCTTGCCGC GGCCGGGTCG GACGGAAATA TTCACATCTT TTCGCTGGGG 
CGCAGCTATG AACACCTGAT CTCGAACAGT AGCCGAGTGA TCAACTTGGC TCCGGTGCTC 
AAGCTGACTC TGTCCTTAGC GGACCAAAGC TGTTGTTCGT CGTATGAGTC CCATAGTGCC 
GTGAAGTTGG CGTGGTCCAA GAGCAAACAC CACTCGATGT TGGCAGCGGG CTATTCGAAC 
GGAACGGTCG CAGTGTGGAA TGCCACGTCA ACTTCGCCTC TGTTAACCGG AACGAAAGAT 
AACGTTCGGG CACTTCTTCC CGTTCATAAG GTGTTCATTC CCGATTCGTG CGTGACCGCT 
GTCGATCTTC ATTATTCGGA AGATTCTCGT TATCTTTTGG TATGTAATGC GGACAGGAAG 
GTCGTTGTCT ACGACCTGAA CACGGGCTAC ATGCCGATGG AGATTTGTAG TTTGAACGCC 
CGTAGTAAAG TTACGACGGC TTGTTGGAAT ATCCACTTCC CGCTGATAAC GATGGCTTTC 
GACGATGTGT ATGCCATCGA CCGGTGCGCT TTGACGTTCC ATCAGCCCCG TGAGATTGGC 
GTGCGGTTGC ATCCGTTGTA TACTCATACG GCGGAAGCAA CTGACATGAG CAACAGCGAT 
CATCTGTCGA CGCACGTGAT CGGTACCGAT GGCGGCGACG TGCTGAGCCA TAAACCGATG 
GCCTTTATTC ACAACATGGG CCAGAAGAAT CCAGCGCAGA TTAAATATGT TTTGACGTCA 
ACAATATCGG TGAAAGTCAA TGAAGAACAG ATGGATAACT CTTACAACAA GTTCGAGAAG 
AACTATTGCG TGCTGTTTTC GGACTTCGAT AAGAACCCCG CTCGAATGGA CCTCAAATCG 
CTACAGGTGA AAACGTTCCG ACGTGCCCTG CTGCACGAAT ACCCGGGCAT CCGGATCAAC 
CAGGTCAAGT GGAATCCCAA CGACAAGTCC CATCAATACT ACGCCATCGG GTACCAGGCT 
GGCTTTGTGC GTGTGCGACC CTTCCGGCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5568954 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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