LOC5568816 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5568816
Entrez Gene ID	5568816
Full Name	protein son of sevenless
Synonyms	AAEL001165
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021845633.1	XP_021701325.1	protein son of sevenless isoform X1
XM_001658132.1	XP_001658182.1	AAEL001165-PA
XM_021845634.1	XP_021701326.1	protein son of sevenless isoform X2
XM_021845636.1	XP_021701328.1	protein son of sevenless isoform X4
XM_021845635.1	XP_021701327.1	protein son of sevenless isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5568816 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5568816 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe04630	XM_001658132.1 Latest version!	Aedes aegypti AAEL001165-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAe122684	XM_021845633.1 Latest version!	Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAe122685	XM_021845634.1 Latest version!	Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAe122686	XM_021845635.1 Latest version!	Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAe122687	XM_021845636.1 Latest version!	Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5568816 ( XM_001658132.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001658182.1 Aedes aegypti AAEL001165-RA partial mRNA.

Price & Availability↑

CloneID	OAe04630
Clone ID Related Accession (Same CDS sequence)	XM_001658132.1
Accession Version	XM_001658132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL001165-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810937). COMPLETENESS: incomplete on the 5' end.

ATGTTCGCTG GCGGCCAGCA TGTATCGATC ACGGATGCGA CAGATTACGA CTTTGAGAAA 
GCGGAAAATG CAGCCAAATG GAAAGGTGTT TTCATAACAT CGCTCAGAAA GGTATTGGAG 
CAAGTGCATC CGTCGTTGCA AGCCCGCGAG GATGCTCTGC TCTACGTCGA AAGTTTATGC 
TTGCGTTTGT TAGCGATGCT GTGTGCAAAA CCACCACCGC ATACAGTATT GGACGTCGAA 
GAACGAATTG GGCGTACGTT TCCTACGCCC ATTGACAAAT GGGCCCTGAG CGAGGCACGT 
GAGACAATAG ACAAATCCAA AAAGAAGAAA TCGGTGCTAC CGGTTGACCG AGTCCATACC 
TTGCTGCAAA AGGAGGTCCT GCAATACAAA ATCGATAGTT CGGTTTCATT GTTCCTGGTG 
GCAGTGCTGG AGTACATTTC GGCGGACATC CTGAAACTAG CTGGCAACTA TGTCAAGAAC 
ATTCGCCACA TCGAGATATC CCGGGAGGAT ATCGAGGTCG CCATGTGCGC TGACAAGGTG 
CTCATGGACA TGTTCTACCA GGGAGACAAT TCGAGCTCGA TGGCACCGAG CCCGTTGCCG 
CCGACGCCTC GTACCAGTCT CAGCTACGAA GAGGTGGTCA AAGAGCTCAT TCACGACGAG 
AAGCAGTACC AGCGGGACCT GCACATGATC ATAAGGGTGT TTCGCGAAGA GCTGGTCAAA 
ATCGTCAAGG ACCCCAAGGA ACTTGACCCA ATCTTCTCGA ACATTATGGA TATCTATGAA 
GTGTCTGTAA CGTTACTGGG TTCACTCGAA GATGTGATCG AGATGTCGCA GGAGCAAACT 
CCACCATGCA TCGGAAGCTG TTTCGAAGAA CTAGCGGAAG CGGCAGAGTT TGATGTGTAC 
GCCAAGTATG CGAAAGATAT CACGTCAGTT CAGGCAAAGG AAGCCTTAGC AAATTTATTG 
TCTAGGCCAG AGGCGAATTC ACTGATGTCG GCTGGTCACG GTTTCAGGGA AGCGGTTAAG 
TTCTATCTAC CAAAACTACT TTTAGGGCCT ATCGGGCATG CACAGTTGTA TTTAGATTAT 
ATTAAAATTT TACTACAGCT TAGTCCCTCG CAGGAGGATA AGGAAAGCTT CGAGCAGGTT 
CAAGGTTTGC TGAAACCCTT GCAGTGCGAG TTGCAAGGCA TATCCTCACT GCTGCCTAAG 
GAATATTTCG CTAGGGTAAA TAGTCGAGCT AGACGTCAGT CGGCAATTGA GAAAACTCGC 
GACTTACAAA ACAGTGTAGA ACATTGGGAC AAGGACGTGG GGCAGTGTTG CAACGAGTTC 
ATTCGGGAGG ATACCCTTGC CAAGCTCAGT TCCGGCAGGC GGCAAACCGA ACGGAAGGTG 
TTCCTATTTG ATGGGTTGTT GGTGTTGTGT AAAACCCGAC GACAGATTGT GCCTGGCACC 
AACTACGATT ACCGATTGAA GGAACGCTTT TTCATGCGCA AGGTGGAGAT CATCGATCGG 
CCTGACACCG AGGAGCTCAA GTATGCTTTC GAGATCTCAC CCCGGGAGCC GACTAGCGTT 
GTACTTATTG CTAAAAATGC GCAACACAAA AACGACTGGA TGGCTGATTT GGTTATGCTG 
AACACGAAAT CCATGTTGGA ACGCATTCTA GACAGTATTC TCCTAGATAT TGAAAAGAAA 
CATCCACTCA AAGTACCTAG TCCGGACATA TATGCATTTG CCGTGCCTGA TTCGCCCTCG 
AATATAGTGC TGGAAGAGCG AGAAGGCACG GGAGTTCCAC TGATCAAGGG CGCCACTCTA 
ACGAAACTGA TCGAACGGCT TACCTATCAC ATTTACGCCG ATCCAATGTT CGTGCGTACC 
TTCCTAACAA CGTACCGATC GTTTTGTTCG CCCAAAGAGC TTCTTAGGTT ACTGGTGGAA 
CGGTTTGACA TACCGGATCC GGCACTAGTG TACGACAGTG CCAGCGACAA GGATCTTGGG 
CCAGACACGG ACAAGTTTCA TAAGAATTCA CAACGGGAAG ACTGGAAACG CTACAAGAAG 
GAGTATGTCC AACCGGTACA GTTCCGGGTA CTGAACGTTC TTCGGCACTG GGTAGACCAT 
CATTTTTACG ACTTTGAACG TGACTCCGAG CTTCTGGAAG AGTTGTTAAA GTTCCTTGAA 
ACGGTACGCG GTAAATCGAT GCGAAAGTGG GTTGACTCAG TGCTGAAGAT TGTGCAACGG 
AAGACCGAAA GCGAAGATAG TCATCGGCAG ATAACGTTTG CCTTCGGCCA TAGTCCACCG 
GCGATCGAGC ATCACCTACC GCTGACTATT GGTGAGGAGG AATATAACCT GCTGATGTTG 
CATCCTCTGG AACTTGCCCG TCAGCTAACG TTGTTAGAAT TTGAAATGTA TAAAAATGTA 
AAACCATCGG AGCTGGTCGG TTCGGTGTGG ACCGGGAAAG ATAAAGAAAC TACTAGTCCT 
AATTTATTAA GAATTATGCA TCACACTACG AATTTCACCC GCTGGATTGA GAAATCAATT 
ATCGAGGCGG AGAATTTCGA CGAACGTGTG GCAATGGCAT CACGTGCAAT AGAGGTGATG 
ATGGTTCTGC AGGATCTAAA CAACTTCAAC GGTGTGCTGT CGATCGTGTC GGCCTTCCAG 
GGGGCGGCCG TCCACCGGCT CAAGCTAACG CTGGAGGAAA TCCCCAAGAG CTACCAGCGG 
GTGCTGGCAG AGTGTCGGGA GCTGAACAAT TCGCACTTCA AAAAGTATCA GGAAAAGCTG 
CGTTCAATAA ATCCACCTTG TGTGCCATTT TTTGGAATGT ATTTAACCAA TATTTTACAT 
ATAGAGGAAG GGAATCCAGA TTTTTTACCA AAGACGAAAC TAATTAACTT CTCTAAGAGA 
AGACGTGTAG CTGAAATCAC TGGTGAAATA CAGCAATATC AAAATCAACC ATACTGTTTG 
AAAGTTGACC CTAGCATTAG GCATTTTCTA GAAAATTTAG ATCCTTTTAC AGGCATGAGT 
GTAACCGAGA TCCAAAACTA TCTCTACGAG GAAAGTAAAA GGATAGAGCC GAAAAATTGT 
CGCCAACCGT TAAAATTCGC ACGCAAGTGG CCAGATATTT CACTAAAGTC TCCCGGCATC 
AAGCCTTCTT CGAGACGACA CAACAGTTCG GCCAACTCGT CATTGAATCT AACGGGTACG 
TTGCCATTTA CGAACAAAGC GCCGCTTGGG AACAGCAACG ATACCGGTGC GGAACAGCAG 
TCACCAACAG CCAGTTCATC GTCGTCTTCC GTGCTCGACT ATTCGATATT TGCTCCTGTG 
AACATTCACT CTTCGTCGTC ATCACAACAC CATCAATACC ATCACCATCA GCAACAAATG 
TACAACTATC AGCATCATTC GCAGGGATCG ATGCAATATT ATCCGGGCCA CCATCAGTCG 
GGTGGTGGCC AGCAGCAAAA CCATCACCAT TATAATCATC ACGGGCATCA GCACAACTTG 
TCCCAGTCAG TGGTCAATCT CGCATCAACG GCAGACCATC AGATGGCGCC GGAGATTCCA 
CGACGTTCCG ATAGTATTAT CCTTACCCCT TCACTATCGT CCCAAGTACA GTACAACCTA 
AACAATAGTA GCTTATCCGA ATCCAGCAGT AATACCGGTG GTGGCAGTAA ACTCGTTCCA 
AGCCCTCGCT ATCCATCCCC GCAGATGCAT CCCATTCACG GTGGCACGGC GTCTACGATG 
GATTTCAACA ACTGTGCCAT CTCGCCGACG CTGTCCACCT CGGTTTCGTC GACGTCCTCG 
GTGTACCAGA CGACGTACAA TAACTCATCG ACGACGGCCG ATTCGATGAT CCTCAACACT 
ACGGCGGCGA GCAACGACTT GGGCCCGCCC CACTCACCGC ACGTTAACGT ACCAAATACA 
CACAATATTC CTCCTCCTAT GCCACCTCCT CTACCTCCTA GGGTTAAACG AAAAGAGTCC 
TCCGGGGAGT CGTCACAATC TTCGCAGGTG CGGCAAGCAC CGGATGCACC CAAGCTTCCT 
CCGAGAGATA TTAGTCCACC TCCGGTACCA CCACGTATCC ACAATCCGTT GCATCAGATA 
TGCGGGAATG TCCAGCAACA GCATCAGCTG TTACACAGCG TAGGAGCTTG TGACAGCTGC 
AACCTCGTTA ATGTATTACC GAAGGACGAA TCTCCGTCTT CATCATCCTC GTCGTCGACG 
CTGACCCGCG ACAACTTGAA CCAGCACAAC ATGAGCGAAC AGCGGCTACT CATGCTACCG 
CATACCAGCA CGATCATGAT GCGACGAAAC TCCGCCATGG ATCGGGCATC GAAGGAGAAA 
GTGTCCAACA TTACCGCATC GCCGTCGCTA TCCGGACTTT CCAGTACAGT GGGGGGTGTG 
GTCGGCGGTG GAAGTACGGC GAGTAGTGGA CCAGTCAGCT TATCCTCATC TAGCGCACCA 
CCACCACCGC CATGTGGAGC GAAGAACAAA TCAGTTCAAA ACTCACCCGT ATCGCAACAG 
CAGCAGCAAC AACCCGCAGT TTGCGGTCGA CGGCCTAGTA CGAATATATC GCCGAGATTT 
TCACCCGGAG AAACCACACC TAAATTACCT CCGAAGCCAA AGCAAACCAA TCTGTCATCA 
CATTCAGATC GAACAATGTT CCCCTATCCG AGCACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001658182.1
CDS	1..4662
Translation	MFAGGQHVSITDATDYDFEKAENAAKWKGVFITSLRKVLEQVHPSLQAREDALLYVESLCLRLLAMLCAKPPPHTVLDVEERIGRTFPTPIDKWALSEARETIDKSKKKKSVLPVDRVHTLLQKEVLQYKIDSSVSLFLVAVLEYISADILKLAGNYVKNIRHIEISREDIEVAMCADKVLMDMFYQGDNSSSMAPSPLPPTPRTSLSYEEVVKELIHDEKQYQRDLHMIIRVFREELVKIVKDPKELDPIFSNIMDIYEVSVTLLGSLEDVIEMSQEQTPPCIGSCFEELAEAAEFDVYAKYAKDITSVQAKEALANLLSRPEANSLMSAGHGFREAVKFYLPKLLLGPIGHAQLYLDYIKILLQLSPSQEDKESFEQVQGLLKPLQCELQGISSLLPKEYFARVNSRARRQSAIEKTRDLQNSVEHWDKDVGQCCNEFIREDTLAKLSSGRRQTERKVFLFDGLLVLCKTRRQIVPGTNYDYRLKERFFMRKVEIIDRPDTEELKYAFEISPREPTSVVLIAKNAQHKNDWMADLVMLNTKSMLERILDSILLDIEKKHPLKVPSPDIYAFAVPDSPSNIVLEEREGTGVPLIKGATLTKLIERLTYHIYADPMFVRTFLTTYRSFCSPKELLRLLVERFDIPDPALVYDSASDKDLGPDTDKFHKNSQREDWKRYKKEYVQPVQFRVLNVLRHWVDHHFYDFERDSELLEELLKFLETVRGKSMRKWVDSVLKIVQRKTESEDSHRQITFAFGHSPPAIEHHLPLTIGEEEYNLLMLHPLELARQLTLLEFEMYKNVKPSELVGSVWTGKDKETTSPNLLRIMHHTTNFTRWIEKSIIEAENFDERVAMASRAIEVMMVLQDLNNFNGVLSIVSAFQGAAVHRLKLTLEEIPKSYQRVLAECRELNNSHFKKYQEKLRSINPPCVPFFGMYLTNILHIEEGNPDFLPKTKLINFSKRRRVAEITGEIQQYQNQPYCLKVDPSIRHFLENLDPFTGMSVTEIQNYLYEESKRIEPKNCRQPLKFARKWPDISLKSPGIKPSSRRHNSSANSSLNLTGTLPFTNKAPLGNSNDTGAEQQSPTASSSSSSVLDYSIFAPVNIHSSSSSQHHQYHHHQQQMYNYQHHSQGSMQYYPGHHQSGGGQQQNHHHYNHHGHQHNLSQSVVNLASTADHQMAPEIPRRSDSIILTPSLSSQVQYNLNNSSLSESSSNTGGGSKLVPSPRYPSPQMHPIHGGTASTMDFNNCAISPTLSTSVSSTSSVYQTTYNNSSTTADSMILNTTAASNDLGPPHSPHVNVPNTHNIPPPMPPPLPPRVKRKESSGESSQSSQVRQAPDAPKLPPRDISPPPVPPRIHNPLHQICGNVQQQHQLLHSVGACDSCNLVNVLPKDESPSSSSSSSTLTRDNLNQHNMSEQRLLMLPHTSTIMMRRNSAMDRASKEKVSNITASPSLSGLSSTVGGVVGGGSTASSGPVSLSSSSAPPPPPCGAKNKSVQNSPVSQQQQQQPAVCGRRPSTNISPRFSPGETTPKLPPKPKQTNLSSHSDRTMFPYPSTN

Target ORF information:

RefSeq Version	XM_001658132.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL001165-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001658132.1

ORF Insert Sequence:

ATGTTCGCTG GCGGCCAGCA TGTATCGATC ACGGATGCGA CAGATTACGA CTTTGAGAAA 
GCGGAAAATG CAGCCAAATG GAAAGGTGTT TTCATAACAT CGCTCAGAAA GGTATTGGAG 
CAAGTGCATC CGTCGTTGCA AGCCCGCGAG GATGCTCTGC TCTACGTCGA AAGTTTATGC 
TTGCGTTTGT TAGCGATGCT GTGTGCAAAA CCACCACCGC ATACAGTATT GGACGTCGAA 
GAACGAATTG GGCGTACGTT TCCTACGCCC ATTGACAAAT GGGCCCTGAG CGAGGCACGT 
GAGACAATAG ACAAATCCAA AAAGAAGAAA TCGGTGCTAC CGGTTGACCG AGTCCATACC 
TTGCTGCAAA AGGAGGTCCT GCAATACAAA ATCGATAGTT CGGTTTCATT GTTCCTGGTG 
GCAGTGCTGG AGTACATTTC GGCGGACATC CTGAAACTAG CTGGCAACTA TGTCAAGAAC 
ATTCGCCACA TCGAGATATC CCGGGAGGAT ATCGAGGTCG CCATGTGCGC TGACAAGGTG 
CTCATGGACA TGTTCTACCA GGGAGACAAT TCGAGCTCGA TGGCACCGAG CCCGTTGCCG 
CCGACGCCTC GTACCAGTCT CAGCTACGAA GAGGTGGTCA AAGAGCTCAT TCACGACGAG 
AAGCAGTACC AGCGGGACCT GCACATGATC ATAAGGGTGT TTCGCGAAGA GCTGGTCAAA 
ATCGTCAAGG ACCCCAAGGA ACTTGACCCA ATCTTCTCGA ACATTATGGA TATCTATGAA 
GTGTCTGTAA CGTTACTGGG TTCACTCGAA GATGTGATCG AGATGTCGCA GGAGCAAACT 
CCACCATGCA TCGGAAGCTG TTTCGAAGAA CTAGCGGAAG CGGCAGAGTT TGATGTGTAC 
GCCAAGTATG CGAAAGATAT CACGTCAGTT CAGGCAAAGG AAGCCTTAGC AAATTTATTG 
TCTAGGCCAG AGGCGAATTC ACTGATGTCG GCTGGTCACG GTTTCAGGGA AGCGGTTAAG 
TTCTATCTAC CAAAACTACT TTTAGGGCCT ATCGGGCATG CACAGTTGTA TTTAGATTAT 
ATTAAAATTT TACTACAGCT TAGTCCCTCG CAGGAGGATA AGGAAAGCTT CGAGCAGGTT 
CAAGGTTTGC TGAAACCCTT GCAGTGCGAG TTGCAAGGCA TATCCTCACT GCTGCCTAAG 
GAATATTTCG CTAGGGTAAA TAGTCGAGCT AGACGTCAGT CGGCAATTGA GAAAACTCGC 
GACTTACAAA ACAGTGTAGA ACATTGGGAC AAGGACGTGG GGCAGTGTTG CAACGAGTTC 
ATTCGGGAGG ATACCCTTGC CAAGCTCAGT TCCGGCAGGC GGCAAACCGA ACGGAAGGTG 
TTCCTATTTG ATGGGTTGTT GGTGTTGTGT AAAACCCGAC GACAGATTGT GCCTGGCACC 
AACTACGATT ACCGATTGAA GGAACGCTTT TTCATGCGCA AGGTGGAGAT CATCGATCGG 
CCTGACACCG AGGAGCTCAA GTATGCTTTC GAGATCTCAC CCCGGGAGCC GACTAGCGTT 
GTACTTATTG CTAAAAATGC GCAACACAAA AACGACTGGA TGGCTGATTT GGTTATGCTG 
AACACGAAAT CCATGTTGGA ACGCATTCTA GACAGTATTC TCCTAGATAT TGAAAAGAAA 
CATCCACTCA AAGTACCTAG TCCGGACATA TATGCATTTG CCGTGCCTGA TTCGCCCTCG 
AATATAGTGC TGGAAGAGCG AGAAGGCACG GGAGTTCCAC TGATCAAGGG CGCCACTCTA 
ACGAAACTGA TCGAACGGCT TACCTATCAC ATTTACGCCG ATCCAATGTT CGTGCGTACC 
TTCCTAACAA CGTACCGATC GTTTTGTTCG CCCAAAGAGC TTCTTAGGTT ACTGGTGGAA 
CGGTTTGACA TACCGGATCC GGCACTAGTG TACGACAGTG CCAGCGACAA GGATCTTGGG 
CCAGACACGG ACAAGTTTCA TAAGAATTCA CAACGGGAAG ACTGGAAACG CTACAAGAAG 
GAGTATGTCC AACCGGTACA GTTCCGGGTA CTGAACGTTC TTCGGCACTG GGTAGACCAT 
CATTTTTACG ACTTTGAACG TGACTCCGAG CTTCTGGAAG AGTTGTTAAA GTTCCTTGAA 
ACGGTACGCG GTAAATCGAT GCGAAAGTGG GTTGACTCAG TGCTGAAGAT TGTGCAACGG 
AAGACCGAAA GCGAAGATAG TCATCGGCAG ATAACGTTTG CCTTCGGCCA TAGTCCACCG 
GCGATCGAGC ATCACCTACC GCTGACTATT GGTGAGGAGG AATATAACCT GCTGATGTTG 
CATCCTCTGG AACTTGCCCG TCAGCTAACG TTGTTAGAAT TTGAAATGTA TAAAAATGTA 
AAACCATCGG AGCTGGTCGG TTCGGTGTGG ACCGGGAAAG ATAAAGAAAC TACTAGTCCT 
AATTTATTAA GAATTATGCA TCACACTACG AATTTCACCC GCTGGATTGA GAAATCAATT 
ATCGAGGCGG AGAATTTCGA CGAACGTGTG GCAATGGCAT CACGTGCAAT AGAGGTGATG 
ATGGTTCTGC AGGATCTAAA CAACTTCAAC GGTGTGCTGT CGATCGTGTC GGCCTTCCAG 
GGGGCGGCCG TCCACCGGCT CAAGCTAACG CTGGAGGAAA TCCCCAAGAG CTACCAGCGG 
GTGCTGGCAG AGTGTCGGGA GCTGAACAAT TCGCACTTCA AAAAGTATCA GGAAAAGCTG 
CGTTCAATAA ATCCACCTTG TGTGCCATTT TTTGGAATGT ATTTAACCAA TATTTTACAT 
ATAGAGGAAG GGAATCCAGA TTTTTTACCA AAGACGAAAC TAATTAACTT CTCTAAGAGA 
AGACGTGTAG CTGAAATCAC TGGTGAAATA CAGCAATATC AAAATCAACC ATACTGTTTG 
AAAGTTGACC CTAGCATTAG GCATTTTCTA GAAAATTTAG ATCCTTTTAC AGGCATGAGT 
GTAACCGAGA TCCAAAACTA TCTCTACGAG GAAAGTAAAA GGATAGAGCC GAAAAATTGT 
CGCCAACCGT TAAAATTCGC ACGCAAGTGG CCAGATATTT CACTAAAGTC TCCCGGCATC 
AAGCCTTCTT CGAGACGACA CAACAGTTCG GCCAACTCGT CATTGAATCT AACGGGTACG 
TTGCCATTTA CGAACAAAGC GCCGCTTGGG AACAGCAACG ATACCGGTGC GGAACAGCAG 
TCACCAACAG CCAGTTCATC GTCGTCTTCC GTGCTCGACT ATTCGATATT TGCTCCTGTG 
AACATTCACT CTTCGTCGTC ATCACAACAC CATCAATACC ATCACCATCA GCAACAAATG 
TACAACTATC AGCATCATTC GCAGGGATCG ATGCAATATT ATCCGGGCCA CCATCAGTCG 
GGTGGTGGCC AGCAGCAAAA CCATCACCAT TATAATCATC ACGGGCATCA GCACAACTTG 
TCCCAGTCAG TGGTCAATCT CGCATCAACG GCAGACCATC AGATGGCGCC GGAGATTCCA 
CGACGTTCCG ATAGTATTAT CCTTACCCCT TCACTATCGT CCCAAGTACA GTACAACCTA 
AACAATAGTA GCTTATCCGA ATCCAGCAGT AATACCGGTG GTGGCAGTAA ACTCGTTCCA 
AGCCCTCGCT ATCCATCCCC GCAGATGCAT CCCATTCACG GTGGCACGGC GTCTACGATG 
GATTTCAACA ACTGTGCCAT CTCGCCGACG CTGTCCACCT CGGTTTCGTC GACGTCCTCG 
GTGTACCAGA CGACGTACAA TAACTCATCG ACGACGGCCG ATTCGATGAT CCTCAACACT 
ACGGCGGCGA GCAACGACTT GGGCCCGCCC CACTCACCGC ACGTTAACGT ACCAAATACA 
CACAATATTC CTCCTCCTAT GCCACCTCCT CTACCTCCTA GGGTTAAACG AAAAGAGTCC 
TCCGGGGAGT CGTCACAATC TTCGCAGGTG CGGCAAGCAC CGGATGCACC CAAGCTTCCT 
CCGAGAGATA TTAGTCCACC TCCGGTACCA CCACGTATCC ACAATCCGTT GCATCAGATA 
TGCGGGAATG TCCAGCAACA GCATCAGCTG TTACACAGCG TAGGAGCTTG TGACAGCTGC 
AACCTCGTTA ATGTATTACC GAAGGACGAA TCTCCGTCTT CATCATCCTC GTCGTCGACG 
CTGACCCGCG ACAACTTGAA CCAGCACAAC ATGAGCGAAC AGCGGCTACT CATGCTACCG 
CATACCAGCA CGATCATGAT GCGACGAAAC TCCGCCATGG ATCGGGCATC GAAGGAGAAA 
GTGTCCAACA TTACCGCATC GCCGTCGCTA TCCGGACTTT CCAGTACAGT GGGGGGTGTG 
GTCGGCGGTG GAAGTACGGC GAGTAGTGGA CCAGTCAGCT TATCCTCATC TAGCGCACCA 
CCACCACCGC CATGTGGAGC GAAGAACAAA TCAGTTCAAA ACTCACCCGT ATCGCAACAG 
CAGCAGCAAC AACCCGCAGT TTGCGGTCGA CGGCCTAGTA CGAATATATC GCCGAGATTT 
TCACCCGGAG AAACCACACC TAAATTACCT CCGAAGCCAA AGCAAACCAA TCTGTCATCA 
CATTCAGATC GAACAATGTT CCCCTATCCG AGCACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568816 ( XM_021845633.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021701325.1 Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAe122684
Clone ID Related Accession (Same CDS sequence)	XM_021845633.1
Accession Version	XM_021845633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	protein son of sevenless isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGCTG GCGGCCAGCA TGTATCGATC ACGGATGCGA CAGATTACGA CTTTGAGAAA 
GCGGAAAATG CAGCCAAATG GAAAGGTGTT TTCATAACAT CGCTCAGAAA GGTATTGGAG 
CAAGTGCATC CGTCGTTGCA AGCCCGCGAG GATGCTCTGC TCTACGTCGA AAGTTTATGC 
TTGCGTCTGT TAGCGATGCT GTGTGCAAAA CCACCACCGC ATACAGTATT GGACGTCGAA 
GAACGAATTG GGCGTACGTT TCCTACGCCC ATTGACAAAT GGGCCCTGAG CGAGGCACGT 
GAGACAATAG ACAAATCCAA AAAGAAGAAA TCGGTGCTAC CGGTTGACCG AGTCCATACC 
TTGCTGCAAA AGGAGGTCCT GCAATACAAA ATCGATAGTT CGGTTTCATT GTTCCTGGTG 
GCAGTGCTGG AGTACATTTC GGCGGACATC CTGAAACTAG CTGGCAACTA TGTCAAGAAC 
ATTCGCCACA TCGAGATATC CCGGGAGGAT ATCGAGGTCG CCATGTGCGC TGACAAGGTG 
CTCATGGACA TGTTCTACCA GGGTGACAAC TCGAGCTCGA TGGCACCGAG CCCGTTGCCG 
CCGACGCCTC GTACCAGTCT CAGCTACGAA GAGGTGGTCA AAGAGCTCAT TCACGACGAG 
AAGCAGTACC AGCGGGACCT GCACATGATC ATAAGGGTGT TTCGCGAGGA GCTGGTCAAA 
ATCGTCAAGG ACCCCAAGGA ACTTGACCCT ATCTTCTCGA ACATTATGGA TATCTATGAA 
GTGTCTGTAA CGTTACTGGG TTCACTCGAA GATGTGATCG AGATGTCGCA GGAGCAAACT 
CCACCATGCA TCGGAAGCTG TTTCGAAGAA CTAGCGGAAG CGGCAGAGTT TGATGTGTAC 
GCCAAGTATG CGAAAGATAT CACGTCAGTT CAGGCAAAGG AAGCCTTAGC AAATTTATTG 
TCTAGGCCAG AGGTACTAGA TAGCAATAGT TTCAGAATTC ATAATCGTCA TCCTAACCCA 
TGCGATCTAT TTCAGGCGAA TTCACTGATG TCGGCTGGTC ACGGTTTCAG GGAAGCGGTT 
AAGTTCTATC TACCAAAACT ACTTTTAGGG CCTATCGGGC ATGCACAGTT GTATTTAGAT 
TATATTAAAA TTTTACTACA GCTTAGTCCC TCGCAGGAGG ATAAGGAAAG CTTCGAGCAG 
GTTCAGGGTT TGTTAAAACC CTTGCAGTGC GAGTTGCAAG GCATATCCTC ACTGCTGCCT 
AAGGAATATT TCGCTAGGGT AAATAGTCGA GCTAGACGTC AGTCGGCAAT TGAGAAGACT 
CGCGACTTAC AAAACAGTGT AGAACATTGG GACAAGGACG TGGGTCAGTG TTGCAACGAG 
TTCATTCGGG AGGATACCCT TGCCAAGCTC AGTTCCGGCA GGCGGCAAAC CGAACGGAAG 
GTGTTCCTAT TTGATGGGTT GTTGGTGTTG TGTAAAACCC GACGACAGAT TGTGCCTGGC 
ACCAACTACG ATTACCGATT GAAGGAACGC TTTTTCATGC GAAAGGTGGA GATCATCGAT 
CGGCCTGACA CCGAGGAGCT CAAGTATGCT TTCGAGATCT CACCCCGGGA GCCGACTAGC 
GTTGTACTTA TTGCTAAAAA TGCGCAACAC AAAAACGACT GGATGGCTGA TTTGGTTATG 
CTGAACACGA AATCCATGTT GGAACGCATT CTAGACAGTA TTCTCCTAGA TATTGAAAAG 
AAACATCCAC TCAAAGTACC TAGTCCGGAC ATATATGCAT TTGCCGTGCC TGATTCGCCC 
TCGAATATAG TGCTGGAAGA GCGAGAAGGC ACGGGAGTTC CACTGATCAA GGGCGCCACT 
CTAACGAAAC TGATCGAACG GCTTACCTAT CACATTTACG CCGATCCAAT GTTCGTGCGT 
ACCTTCCTAA CAACGTACCG ATCGTTTTGT TCGCCCACAG AGCTTCTTAG GTTACTGGTG 
GAACGGTTTG ACATACCGGA TCCGGCACTA GTGTACGACA GTGCCAGCGA CAAGGATCTA 
GGGCCAGACA CGGACAAGTT TCATAAAAAT TCACAACGGG AAGACTGGAA ACGCTATAAG 
AAGGAGTATG TCCAACCGGT ACAGTTCCGG GTACTGAACG TTCTTCGGCA CTGGGTAGAC 
CATCATTTTT ACGACTTTGA ACGTGACTCC GAGCTTCTGG AAGAGTTGTT AAAGTTCCTT 
GAAACGGTAC GCGGTAAATC GATGCGAAAG TGGGTTGACT CAGTGCTGAA GATTGTGCAA 
CGGAAGACCG AAAGCGAAGA TAGTCATCGG CAGATAACGT TTGCCTTCGG CCATAGTCCA 
CCGGCGATCG AGCATCACCT ACCGCTGACT ATTGGTGAGG AGGAATATAA CCTGCTGATG 
TTGCATCCCC TGGAACTTGC CCGTCAGCTA ACGTTGTTAG AATTTGAAAT GTATAAAAAT 
GTAAAACCAT CGGAACTGGT CGGTTCGGTG TGGACCGGGA AAGATAAAGA AACTACTAGT 
CCTAATTTAT TAAGAATTAT GCATCACACT ACGAATTTCA CCCGCTGGAT TGAGAAATCA 
ATTATCGAGG CGGAGAATTT CGACGAACGT GTGGCAATGG CATCACGTGC AATAGAGGTG 
ATGATGGTTC TGCAGGATCT AAACAACTTC AACGGTGTGC TGTCGATCGT GTCGGCCTTC 
CAGGGGGCGG CCGTCCACCG GCTCAAGCTA ACGCTGGAGG AAATCCCCAA GAGCTACCAG 
CGGGTGCTGG CAGAGTGTCG GGAGCTGAAC AATTCGCACT TCAAAAAGTA TCAGGAAAAG 
CTGCGTTCAA TAAATCCACC TTGTGTGCCA TTTTTTGGAA TGTATTTAAC CAATATTTTA 
CATATAGAGG AAGGGAATCC AGATTTTTTA CCAAAGACGA AACTAATTAA CTTCTCTAAG 
AGAAGACGTG TAGCTGAAAT CACTGGTGAA ATACAGCAAT ATCAAAATCA ACCATACTGT 
TTGAAAGTTG ACCCTAGCAT TAGGCATTTT CTAGAAAATT TAGATCCTTT TACAGGCATG 
AGTGTAACCG AGATCCAAAA CTATCTCTAC GAGGAAAGTA AAAGGATAGA GCCGAAAAAT 
TGTCGCCAAC CGTTAAAATT CGCACGCAAG TGGCCAGATA TTTCACTAAA GTCTCCCGGC 
ATCAAGCCTT CTTCGAGACG ACACAACAGT TCGGCCAACT CGTCATTGAA TCTAACGGGT 
ACGTTGCCAT TTACGAACAA AGCGCCGCTT GGGAACAGCA ACGATACCGG TGCGGAACAG 
CAGTCACCAA CAGCCAGTTC ATCGTCGTCT TCCGTGCTCG ACTATTCGAT ATTTGCTCCT 
GTGAACATTC ACTCTTCGTC GTCATCACAA CACCATCAAT ACCATCACCA TCAGCAACAA 
ATGTACAACT ATCAGCATCA TTCGCAGGGA TCGATGCAAT ATTATCCGGG CCACCATCAG 
TCGGGTGGTG GCCAGCAGCA AAACCATCAC CATTATAATC ATCACGGGCA TCAGCACAAC 
TTGTCCCAGT CAGTGGTCAA TCTCGCATCA ACGGCAGACC ATCAGATGGC GCCGGAGATT 
CCACGACGTT CCGATAGTAT TATCCTTACC CCTTCACTAT CGTCCCAAGT ACAGTACAAC 
CTAAACAATA GTAGCTTATC CGAATCCAGC AGTAATACCG GTGGTGGCAG TAAACTCGTT 
CCAAGCCCTC GCTATCCATC GTCATCAATG AGTGCGCTTT CGTCTTCGTC CTCCGGCATA 
AGTGTTTCTT CGTTCTATGG AATCATGAGC GCATCGAACA GTGATGGTAA CAGCAACAAT 
AATCCTACTA CTGCTAGCTT TAAACATACG CCATTGCTTA GTCAGAACAA AGTAGTTGCC 
AATACGTGTG CTGAAGACAG TCCCCATTCG ATTCTTTCAG CGTCTGGTGT AGGGGGCGGA 
GGAAGTCTCA GCATGAGCAG CTCGTCTGCA ACCAACGCCG CTAGTATTGT TTCGCTAAGT 
GAGGGTTCAG CCGTTCCTCA ACCCAACACC GGTACAGCCA TCTCGCCGTG TACGACGATG 
TCTTCGCCAT CGGCCAGCTC GTCCTCGACC GTGACTGCCG TGGGAACAAC GGTTACTGCA 
TCACCAGCAC CAGCATCGTC CGGTGCTGCC GACATTCCAC CTGCGATTAG TCCACGCACT 
GACAAGCCGG TATACCCGCC GGGACCGCCA CCCTTACGGA ACCATGGAGG TGGGGCAACC 
GAGTTTTGCT ACAACACTTC CGGACTACCG ACATCACCGC TAGCAACTAG CTGTAACTTT 
AGGATTAGTT CATCCAACAT TGGAAAGAAT CAAAACTGTG ATTTTACGAC GGCGAGCAGC 
GCGGGCAACC GCTCGTCGTG TGACAATTCG CCGATTTTCC CCTCGAGCCC GCAGATGCAT 
CCCATTCACG GTGGCACGGC GTCTACGATG GATTTCAACA ACTGTGCCAT CTCGCCGACG 
CTGTCCACCT CGGTTTCGTC GACGTCCTCG GTGTACCAGA CGACGTACAA TAACTCATCG 
ACGACGGCCG ATTCGATGAT CCTCAACACT ACGGCGGCGA GCAACGACTT GGGCCCGCCC 
CACTCACCGC ACGTTAACGT ACCAAATACA CACAATATTC CTCCTCCTAT GCCACCTCCT 
CTACCTCCTA GGGTTAAACG AAAAGAGTCC TCCGGGGAGT CGTCACAATC TTCGCAGGTG 
CGGCAAGCAC CGGATGCACC CAAGCTTCCT CCGAGAGATA TTAGTCCACC TCCGGTACCA 
CCACGTATCC ACAATCCGTC GCATCAGATA TGCGGGAATG TCCAGCAACA GCATCAGCTG 
TTACACAGCG TAGGAGCTTG TGACAGCTGC AACCTCGTTA ATGTATTACC GAAGGACGAA 
TCTCCGTCTT CATCATCCTC GTCGTCGACG CTGACCCGCG ACAACTTGAA CCAGCACAAC 
ATGAGCGAAC AGCGGCTACT CATGCTACCG CATACCAGCA CGATCATGAT GCGACGAAAC 
TCCGCCATGG ATCGGGCATC GAAGGAGAAA GTGTCCAACA TTACCGCATC GCCGTCGCTA 
TCCGGACTTT CCAGTACAGT GGGGGGTGTG GTCGGCGGTG GAAGTACGGC GAGTAGTGGA 
CCAGTCAGCT TATCCTCATC TAGCGCACCA CCACCGCCAT GTGGAGCGAA GAACAAATCA 
GTTCAAAACT CACCCGTATC GCAACAGCAG CAGCAACAAC CCGCAGTTTG CGGTCGACGG 
CCTAGTACGA ATATATCGCC GAGATTTTCA CCCGGAGAAA CCACACCTAA ATTACCTCCG 
AAGCCAAAGC AAACCAATCT GTCATCACAT TCAGATCGAA CAATGTTCCC CTATCCGAGC 
ACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021701325.1
CDS	827..6235
Translation	MFAGGQHVSITDATDYDFEKAENAAKWKGVFITSLRKVLEQVHPSLQAREDALLYVESLCLRLLAMLCAKPPPHTVLDVEERIGRTFPTPIDKWALSEARETIDKSKKKKSVLPVDRVHTLLQKEVLQYKIDSSVSLFLVAVLEYISADILKLAGNYVKNIRHIEISREDIEVAMCADKVLMDMFYQGDNSSSMAPSPLPPTPRTSLSYEEVVKELIHDEKQYQRDLHMIIRVFREELVKIVKDPKELDPIFSNIMDIYEVSVTLLGSLEDVIEMSQEQTPPCIGSCFEELAEAAEFDVYAKYAKDITSVQAKEALANLLSRPEVLDSNSFRIHNRHPNPCDLFQANSLMSAGHGFREAVKFYLPKLLLGPIGHAQLYLDYIKILLQLSPSQEDKESFEQVQGLLKPLQCELQGISSLLPKEYFARVNSRARRQSAIEKTRDLQNSVEHWDKDVGQCCNEFIREDTLAKLSSGRRQTERKVFLFDGLLVLCKTRRQIVPGTNYDYRLKERFFMRKVEIIDRPDTEELKYAFEISPREPTSVVLIAKNAQHKNDWMADLVMLNTKSMLERILDSILLDIEKKHPLKVPSPDIYAFAVPDSPSNIVLEEREGTGVPLIKGATLTKLIERLTYHIYADPMFVRTFLTTYRSFCSPTELLRLLVERFDIPDPALVYDSASDKDLGPDTDKFHKNSQREDWKRYKKEYVQPVQFRVLNVLRHWVDHHFYDFERDSELLEELLKFLETVRGKSMRKWVDSVLKIVQRKTESEDSHRQITFAFGHSPPAIEHHLPLTIGEEEYNLLMLHPLELARQLTLLEFEMYKNVKPSELVGSVWTGKDKETTSPNLLRIMHHTTNFTRWIEKSIIEAENFDERVAMASRAIEVMMVLQDLNNFNGVLSIVSAFQGAAVHRLKLTLEEIPKSYQRVLAECRELNNSHFKKYQEKLRSINPPCVPFFGMYLTNILHIEEGNPDFLPKTKLINFSKRRRVAEITGEIQQYQNQPYCLKVDPSIRHFLENLDPFTGMSVTEIQNYLYEESKRIEPKNCRQPLKFARKWPDISLKSPGIKPSSRRHNSSANSSLNLTGTLPFTNKAPLGNSNDTGAEQQSPTASSSSSSVLDYSIFAPVNIHSSSSSQHHQYHHHQQQMYNYQHHSQGSMQYYPGHHQSGGGQQQNHHHYNHHGHQHNLSQSVVNLASTADHQMAPEIPRRSDSIILTPSLSSQVQYNLNNSSLSESSSNTGGGSKLVPSPRYPSSSMSALSSSSSGISVSSFYGIMSASNSDGNSNNNPTTASFKHTPLLSQNKVVANTCAEDSPHSILSASGVGGGGSLSMSSSSATNAASIVSLSEGSAVPQPNTGTAISPCTTMSSPSASSSSTVTAVGTTVTASPAPASSGAADIPPAISPRTDKPVYPPGPPPLRNHGGGATEFCYNTSGLPTSPLATSCNFRISSSNIGKNQNCDFTTASSAGNRSSCDNSPIFPSSPQMHPIHGGTASTMDFNNCAISPTLSTSVSSTSSVYQTTYNNSSTTADSMILNTTAASNDLGPPHSPHVNVPNTHNIPPPMPPPLPPRVKRKESSGESSQSSQVRQAPDAPKLPPRDISPPPVPPRIHNPSHQICGNVQQQHQLLHSVGACDSCNLVNVLPKDESPSSSSSSSTLTRDNLNQHNMSEQRLLMLPHTSTIMMRRNSAMDRASKEKVSNITASPSLSGLSSTVGGVVGGGSTASSGPVSLSSSSAPPPPCGAKNKSVQNSPVSQQQQQQPAVCGRRPSTNISPRFSPGETTPKLPPKPKQTNLSSHSDRTMFPYPSTN

Target ORF information:

RefSeq Version	XM_021845633.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021845633.1

ORF Insert Sequence:

ATGTTCGCTG GCGGCCAGCA TGTATCGATC ACGGATGCGA CAGATTACGA CTTTGAGAAA 
GCGGAAAATG CAGCCAAATG GAAAGGTGTT TTCATAACAT CGCTCAGAAA GGTATTGGAG 
CAAGTGCATC CGTCGTTGCA AGCCCGCGAG GATGCTCTGC TCTACGTCGA AAGTTTATGC 
TTGCGTCTGT TAGCGATGCT GTGTGCAAAA CCACCACCGC ATACAGTATT GGACGTCGAA 
GAACGAATTG GGCGTACGTT TCCTACGCCC ATTGACAAAT GGGCCCTGAG CGAGGCACGT 
GAGACAATAG ACAAATCCAA AAAGAAGAAA TCGGTGCTAC CGGTTGACCG AGTCCATACC 
TTGCTGCAAA AGGAGGTCCT GCAATACAAA ATCGATAGTT CGGTTTCATT GTTCCTGGTG 
GCAGTGCTGG AGTACATTTC GGCGGACATC CTGAAACTAG CTGGCAACTA TGTCAAGAAC 
ATTCGCCACA TCGAGATATC CCGGGAGGAT ATCGAGGTCG CCATGTGCGC TGACAAGGTG 
CTCATGGACA TGTTCTACCA GGGTGACAAC TCGAGCTCGA TGGCACCGAG CCCGTTGCCG 
CCGACGCCTC GTACCAGTCT CAGCTACGAA GAGGTGGTCA AAGAGCTCAT TCACGACGAG 
AAGCAGTACC AGCGGGACCT GCACATGATC ATAAGGGTGT TTCGCGAGGA GCTGGTCAAA 
ATCGTCAAGG ACCCCAAGGA ACTTGACCCT ATCTTCTCGA ACATTATGGA TATCTATGAA 
GTGTCTGTAA CGTTACTGGG TTCACTCGAA GATGTGATCG AGATGTCGCA GGAGCAAACT 
CCACCATGCA TCGGAAGCTG TTTCGAAGAA CTAGCGGAAG CGGCAGAGTT TGATGTGTAC 
GCCAAGTATG CGAAAGATAT CACGTCAGTT CAGGCAAAGG AAGCCTTAGC AAATTTATTG 
TCTAGGCCAG AGGTACTAGA TAGCAATAGT TTCAGAATTC ATAATCGTCA TCCTAACCCA 
TGCGATCTAT TTCAGGCGAA TTCACTGATG TCGGCTGGTC ACGGTTTCAG GGAAGCGGTT 
AAGTTCTATC TACCAAAACT ACTTTTAGGG CCTATCGGGC ATGCACAGTT GTATTTAGAT 
TATATTAAAA TTTTACTACA GCTTAGTCCC TCGCAGGAGG ATAAGGAAAG CTTCGAGCAG 
GTTCAGGGTT TGTTAAAACC CTTGCAGTGC GAGTTGCAAG GCATATCCTC ACTGCTGCCT 
AAGGAATATT TCGCTAGGGT AAATAGTCGA GCTAGACGTC AGTCGGCAAT TGAGAAGACT 
CGCGACTTAC AAAACAGTGT AGAACATTGG GACAAGGACG TGGGTCAGTG TTGCAACGAG 
TTCATTCGGG AGGATACCCT TGCCAAGCTC AGTTCCGGCA GGCGGCAAAC CGAACGGAAG 
GTGTTCCTAT TTGATGGGTT GTTGGTGTTG TGTAAAACCC GACGACAGAT TGTGCCTGGC 
ACCAACTACG ATTACCGATT GAAGGAACGC TTTTTCATGC GAAAGGTGGA GATCATCGAT 
CGGCCTGACA CCGAGGAGCT CAAGTATGCT TTCGAGATCT CACCCCGGGA GCCGACTAGC 
GTTGTACTTA TTGCTAAAAA TGCGCAACAC AAAAACGACT GGATGGCTGA TTTGGTTATG 
CTGAACACGA AATCCATGTT GGAACGCATT CTAGACAGTA TTCTCCTAGA TATTGAAAAG 
AAACATCCAC TCAAAGTACC TAGTCCGGAC ATATATGCAT TTGCCGTGCC TGATTCGCCC 
TCGAATATAG TGCTGGAAGA GCGAGAAGGC ACGGGAGTTC CACTGATCAA GGGCGCCACT 
CTAACGAAAC TGATCGAACG GCTTACCTAT CACATTTACG CCGATCCAAT GTTCGTGCGT 
ACCTTCCTAA CAACGTACCG ATCGTTTTGT TCGCCCACAG AGCTTCTTAG GTTACTGGTG 
GAACGGTTTG ACATACCGGA TCCGGCACTA GTGTACGACA GTGCCAGCGA CAAGGATCTA 
GGGCCAGACA CGGACAAGTT TCATAAAAAT TCACAACGGG AAGACTGGAA ACGCTATAAG 
AAGGAGTATG TCCAACCGGT ACAGTTCCGG GTACTGAACG TTCTTCGGCA CTGGGTAGAC 
CATCATTTTT ACGACTTTGA ACGTGACTCC GAGCTTCTGG AAGAGTTGTT AAAGTTCCTT 
GAAACGGTAC GCGGTAAATC GATGCGAAAG TGGGTTGACT CAGTGCTGAA GATTGTGCAA 
CGGAAGACCG AAAGCGAAGA TAGTCATCGG CAGATAACGT TTGCCTTCGG CCATAGTCCA 
CCGGCGATCG AGCATCACCT ACCGCTGACT ATTGGTGAGG AGGAATATAA CCTGCTGATG 
TTGCATCCCC TGGAACTTGC CCGTCAGCTA ACGTTGTTAG AATTTGAAAT GTATAAAAAT 
GTAAAACCAT CGGAACTGGT CGGTTCGGTG TGGACCGGGA AAGATAAAGA AACTACTAGT 
CCTAATTTAT TAAGAATTAT GCATCACACT ACGAATTTCA CCCGCTGGAT TGAGAAATCA 
ATTATCGAGG CGGAGAATTT CGACGAACGT GTGGCAATGG CATCACGTGC AATAGAGGTG 
ATGATGGTTC TGCAGGATCT AAACAACTTC AACGGTGTGC TGTCGATCGT GTCGGCCTTC 
CAGGGGGCGG CCGTCCACCG GCTCAAGCTA ACGCTGGAGG AAATCCCCAA GAGCTACCAG 
CGGGTGCTGG CAGAGTGTCG GGAGCTGAAC AATTCGCACT TCAAAAAGTA TCAGGAAAAG 
CTGCGTTCAA TAAATCCACC TTGTGTGCCA TTTTTTGGAA TGTATTTAAC CAATATTTTA 
CATATAGAGG AAGGGAATCC AGATTTTTTA CCAAAGACGA AACTAATTAA CTTCTCTAAG 
AGAAGACGTG TAGCTGAAAT CACTGGTGAA ATACAGCAAT ATCAAAATCA ACCATACTGT 
TTGAAAGTTG ACCCTAGCAT TAGGCATTTT CTAGAAAATT TAGATCCTTT TACAGGCATG 
AGTGTAACCG AGATCCAAAA CTATCTCTAC GAGGAAAGTA AAAGGATAGA GCCGAAAAAT 
TGTCGCCAAC CGTTAAAATT CGCACGCAAG TGGCCAGATA TTTCACTAAA GTCTCCCGGC 
ATCAAGCCTT CTTCGAGACG ACACAACAGT TCGGCCAACT CGTCATTGAA TCTAACGGGT 
ACGTTGCCAT TTACGAACAA AGCGCCGCTT GGGAACAGCA ACGATACCGG TGCGGAACAG 
CAGTCACCAA CAGCCAGTTC ATCGTCGTCT TCCGTGCTCG ACTATTCGAT ATTTGCTCCT 
GTGAACATTC ACTCTTCGTC GTCATCACAA CACCATCAAT ACCATCACCA TCAGCAACAA 
ATGTACAACT ATCAGCATCA TTCGCAGGGA TCGATGCAAT ATTATCCGGG CCACCATCAG 
TCGGGTGGTG GCCAGCAGCA AAACCATCAC CATTATAATC ATCACGGGCA TCAGCACAAC 
TTGTCCCAGT CAGTGGTCAA TCTCGCATCA ACGGCAGACC ATCAGATGGC GCCGGAGATT 
CCACGACGTT CCGATAGTAT TATCCTTACC CCTTCACTAT CGTCCCAAGT ACAGTACAAC 
CTAAACAATA GTAGCTTATC CGAATCCAGC AGTAATACCG GTGGTGGCAG TAAACTCGTT 
CCAAGCCCTC GCTATCCATC GTCATCAATG AGTGCGCTTT CGTCTTCGTC CTCCGGCATA 
AGTGTTTCTT CGTTCTATGG AATCATGAGC GCATCGAACA GTGATGGTAA CAGCAACAAT 
AATCCTACTA CTGCTAGCTT TAAACATACG CCATTGCTTA GTCAGAACAA AGTAGTTGCC 
AATACGTGTG CTGAAGACAG TCCCCATTCG ATTCTTTCAG CGTCTGGTGT AGGGGGCGGA 
GGAAGTCTCA GCATGAGCAG CTCGTCTGCA ACCAACGCCG CTAGTATTGT TTCGCTAAGT 
GAGGGTTCAG CCGTTCCTCA ACCCAACACC GGTACAGCCA TCTCGCCGTG TACGACGATG 
TCTTCGCCAT CGGCCAGCTC GTCCTCGACC GTGACTGCCG TGGGAACAAC GGTTACTGCA 
TCACCAGCAC CAGCATCGTC CGGTGCTGCC GACATTCCAC CTGCGATTAG TCCACGCACT 
GACAAGCCGG TATACCCGCC GGGACCGCCA CCCTTACGGA ACCATGGAGG TGGGGCAACC 
GAGTTTTGCT ACAACACTTC CGGACTACCG ACATCACCGC TAGCAACTAG CTGTAACTTT 
AGGATTAGTT CATCCAACAT TGGAAAGAAT CAAAACTGTG ATTTTACGAC GGCGAGCAGC 
GCGGGCAACC GCTCGTCGTG TGACAATTCG CCGATTTTCC CCTCGAGCCC GCAGATGCAT 
CCCATTCACG GTGGCACGGC GTCTACGATG GATTTCAACA ACTGTGCCAT CTCGCCGACG 
CTGTCCACCT CGGTTTCGTC GACGTCCTCG GTGTACCAGA CGACGTACAA TAACTCATCG 
ACGACGGCCG ATTCGATGAT CCTCAACACT ACGGCGGCGA GCAACGACTT GGGCCCGCCC 
CACTCACCGC ACGTTAACGT ACCAAATACA CACAATATTC CTCCTCCTAT GCCACCTCCT 
CTACCTCCTA GGGTTAAACG AAAAGAGTCC TCCGGGGAGT CGTCACAATC TTCGCAGGTG 
CGGCAAGCAC CGGATGCACC CAAGCTTCCT CCGAGAGATA TTAGTCCACC TCCGGTACCA 
CCACGTATCC ACAATCCGTC GCATCAGATA TGCGGGAATG TCCAGCAACA GCATCAGCTG 
TTACACAGCG TAGGAGCTTG TGACAGCTGC AACCTCGTTA ATGTATTACC GAAGGACGAA 
TCTCCGTCTT CATCATCCTC GTCGTCGACG CTGACCCGCG ACAACTTGAA CCAGCACAAC 
ATGAGCGAAC AGCGGCTACT CATGCTACCG CATACCAGCA CGATCATGAT GCGACGAAAC 
TCCGCCATGG ATCGGGCATC GAAGGAGAAA GTGTCCAACA TTACCGCATC GCCGTCGCTA 
TCCGGACTTT CCAGTACAGT GGGGGGTGTG GTCGGCGGTG GAAGTACGGC GAGTAGTGGA 
CCAGTCAGCT TATCCTCATC TAGCGCACCA CCACCGCCAT GTGGAGCGAA GAACAAATCA 
GTTCAAAACT CACCCGTATC GCAACAGCAG CAGCAACAAC CCGCAGTTTG CGGTCGACGG 
CCTAGTACGA ATATATCGCC GAGATTTTCA CCCGGAGAAA CCACACCTAA ATTACCTCCG 
AAGCCAAAGC AAACCAATCT GTCATCACAT TCAGATCGAA CAATGTTCCC CTATCCGAGC 
ACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568816 ( XM_021845634.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021701326.1 Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X2, mRNA.

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CloneID	OAe122685
Clone ID Related Accession (Same CDS sequence)	XM_021845634.1
Accession Version	XM_021845634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	protein son of sevenless isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGCTG GCGGCCAGCA TGTATCGATC ACGGATGCGA CAGATTACGA CTTTGAGAAA 
GCGGAAAATG CAGCCAAATG GAAAGGTGTT TTCATAACAT CGCTCAGAAA GGTATTGGAG 
CAAGTGCATC CGTCGTTGCA AGCCCGCGAG GATGCTCTGC TCTACGTCGA AAGTTTATGC 
TTGCGTCTGT TAGCGATGCT GTGTGCAAAA CCACCACCGC ATACAGTATT GGACGTCGAA 
GAACGAATTG GGCGTACGTT TCCTACGCCC ATTGACAAAT GGGCCCTGAG CGAGGCACGT 
GAGACAATAG ACAAATCCAA AAAGAAGAAA TCGGTGCTAC CGGTTGACCG AGTCCATACC 
TTGCTGCAAA AGGAGGTCCT GCAATACAAA ATCGATAGTT CGGTTTCATT GTTCCTGGTG 
GCAGTGCTGG AGTACATTTC GGCGGACATC CTGAAACTAG CTGGCAACTA TGTCAAGAAC 
ATTCGCCACA TCGAGATATC CCGGGAGGAT ATCGAGGTCG CCATGTGCGC TGACAAGGTG 
CTCATGGACA TGTTCTACCA GGGTGACAAC TCGAGCTCGA TGGCACCGAG CCCGTTGCCG 
CCGACGCCTC GTACCAGTCT CAGCTACGAA GAGGTGGTCA AAGAGCTCAT TCACGACGAG 
AAGCAGTACC AGCGGGACCT GCACATGATC ATAAGGGTGT TTCGCGAGGA GCTGGTCAAA 
ATCGTCAAGG ACCCCAAGGA ACTTGACCCT ATCTTCTCGA ACATTATGGA TATCTATGAA 
GTGTCTGTAA CGTTACTGGG TTCACTCGAA GATGTGATCG AGATGTCGCA GGAGCAAACT 
CCACCATGCA TCGGAAGCTG TTTCGAAGAA CTAGCGGAAG CGGCAGAGTT TGATGTGTAC 
GCCAAGTATG CGAAAGATAT CACGTCAGTT CAGGCAAAGG AAGCCTTAGC AAATTTATTG 
TCTAGGCCAG AGGCGAATTC ACTGATGTCG GCTGGTCACG GTTTCAGGGA AGCGGTTAAG 
TTCTATCTAC CAAAACTACT TTTAGGGCCT ATCGGGCATG CACAGTTGTA TTTAGATTAT 
ATTAAAATTT TACTACAGCT TAGTCCCTCG CAGGAGGATA AGGAAAGCTT CGAGCAGGTT 
CAGGGTTTGT TAAAACCCTT GCAGTGCGAG TTGCAAGGCA TATCCTCACT GCTGCCTAAG 
GAATATTTCG CTAGGGTAAA TAGTCGAGCT AGACGTCAGT CGGCAATTGA GAAGACTCGC 
GACTTACAAA ACAGTGTAGA ACATTGGGAC AAGGACGTGG GTCAGTGTTG CAACGAGTTC 
ATTCGGGAGG ATACCCTTGC CAAGCTCAGT TCCGGCAGGC GGCAAACCGA ACGGAAGGTG 
TTCCTATTTG ATGGGTTGTT GGTGTTGTGT AAAACCCGAC GACAGATTGT GCCTGGCACC 
AACTACGATT ACCGATTGAA GGAACGCTTT TTCATGCGAA AGGTGGAGAT CATCGATCGG 
CCTGACACCG AGGAGCTCAA GTATGCTTTC GAGATCTCAC CCCGGGAGCC GACTAGCGTT 
GTACTTATTG CTAAAAATGC GCAACACAAA AACGACTGGA TGGCTGATTT GGTTATGCTG 
AACACGAAAT CCATGTTGGA ACGCATTCTA GACAGTATTC TCCTAGATAT TGAAAAGAAA 
CATCCACTCA AAGTACCTAG TCCGGACATA TATGCATTTG CCGTGCCTGA TTCGCCCTCG 
AATATAGTGC TGGAAGAGCG AGAAGGCACG GGAGTTCCAC TGATCAAGGG CGCCACTCTA 
ACGAAACTGA TCGAACGGCT TACCTATCAC ATTTACGCCG ATCCAATGTT CGTGCGTACC 
TTCCTAACAA CGTACCGATC GTTTTGTTCG CCCACAGAGC TTCTTAGGTT ACTGGTGGAA 
CGGTTTGACA TACCGGATCC GGCACTAGTG TACGACAGTG CCAGCGACAA GGATCTAGGG 
CCAGACACGG ACAAGTTTCA TAAAAATTCA CAACGGGAAG ACTGGAAACG CTATAAGAAG 
GAGTATGTCC AACCGGTACA GTTCCGGGTA CTGAACGTTC TTCGGCACTG GGTAGACCAT 
CATTTTTACG ACTTTGAACG TGACTCCGAG CTTCTGGAAG AGTTGTTAAA GTTCCTTGAA 
ACGGTACGCG GTAAATCGAT GCGAAAGTGG GTTGACTCAG TGCTGAAGAT TGTGCAACGG 
AAGACCGAAA GCGAAGATAG TCATCGGCAG ATAACGTTTG CCTTCGGCCA TAGTCCACCG 
GCGATCGAGC ATCACCTACC GCTGACTATT GGTGAGGAGG AATATAACCT GCTGATGTTG 
CATCCCCTGG AACTTGCCCG TCAGCTAACG TTGTTAGAAT TTGAAATGTA TAAAAATGTA 
AAACCATCGG AACTGGTCGG TTCGGTGTGG ACCGGGAAAG ATAAAGAAAC TACTAGTCCT 
AATTTATTAA GAATTATGCA TCACACTACG AATTTCACCC GCTGGATTGA GAAATCAATT 
ATCGAGGCGG AGAATTTCGA CGAACGTGTG GCAATGGCAT CACGTGCAAT AGAGGTGATG 
ATGGTTCTGC AGGATCTAAA CAACTTCAAC GGTGTGCTGT CGATCGTGTC GGCCTTCCAG 
GGGGCGGCCG TCCACCGGCT CAAGCTAACG CTGGAGGAAA TCCCCAAGAG CTACCAGCGG 
GTGCTGGCAG AGTGTCGGGA GCTGAACAAT TCGCACTTCA AAAAGTATCA GGAAAAGCTG 
CGTTCAATAA ATCCACCTTG TGTGCCATTT TTTGGAATGT ATTTAACCAA TATTTTACAT 
ATAGAGGAAG GGAATCCAGA TTTTTTACCA AAGACGAAAC TAATTAACTT CTCTAAGAGA 
AGACGTGTAG CTGAAATCAC TGGTGAAATA CAGCAATATC AAAATCAACC ATACTGTTTG 
AAAGTTGACC CTAGCATTAG GCATTTTCTA GAAAATTTAG ATCCTTTTAC AGGCATGAGT 
GTAACCGAGA TCCAAAACTA TCTCTACGAG GAAAGTAAAA GGATAGAGCC GAAAAATTGT 
CGCCAACCGT TAAAATTCGC ACGCAAGTGG CCAGATATTT CACTAAAGTC TCCCGGCATC 
AAGCCTTCTT CGAGACGACA CAACAGTTCG GCCAACTCGT CATTGAATCT AACGGGTACG 
TTGCCATTTA CGAACAAAGC GCCGCTTGGG AACAGCAACG ATACCGGTGC GGAACAGCAG 
TCACCAACAG CCAGTTCATC GTCGTCTTCC GTGCTCGACT ATTCGATATT TGCTCCTGTG 
AACATTCACT CTTCGTCGTC ATCACAACAC CATCAATACC ATCACCATCA GCAACAAATG 
TACAACTATC AGCATCATTC GCAGGGATCG ATGCAATATT ATCCGGGCCA CCATCAGTCG 
GGTGGTGGCC AGCAGCAAAA CCATCACCAT TATAATCATC ACGGGCATCA GCACAACTTG 
TCCCAGTCAG TGGTCAATCT CGCATCAACG GCAGACCATC AGATGGCGCC GGAGATTCCA 
CGACGTTCCG ATAGTATTAT CCTTACCCCT TCACTATCGT CCCAAGTACA GTACAACCTA 
AACAATAGTA GCTTATCCGA ATCCAGCAGT AATACCGGTG GTGGCAGTAA ACTCGTTCCA 
AGCCCTCGCT ATCCATCGTC ATCAATGAGT GCGCTTTCGT CTTCGTCCTC CGGCATAAGT 
GTTTCTTCGT TCTATGGAAT CATGAGCGCA TCGAACAGTG ATGGTAACAG CAACAATAAT 
CCTACTACTG CTAGCTTTAA ACATACGCCA TTGCTTAGTC AGAACAAAGT AGTTGCCAAT 
ACGTGTGCTG AAGACAGTCC CCATTCGATT CTTTCAGCGT CTGGTGTAGG GGGCGGAGGA 
AGTCTCAGCA TGAGCAGCTC GTCTGCAACC AACGCCGCTA GTATTGTTTC GCTAAGTGAG 
GGTTCAGCCG TTCCTCAACC CAACACCGGT ACAGCCATCT CGCCGTGTAC GACGATGTCT 
TCGCCATCGG CCAGCTCGTC CTCGACCGTG ACTGCCGTGG GAACAACGGT TACTGCATCA 
CCAGCACCAG CATCGTCCGG TGCTGCCGAC ATTCCACCTG CGATTAGTCC ACGCACTGAC 
AAGCCGGTAT ACCCGCCGGG ACCGCCACCC TTACGGAACC ATGGAGGTGG GGCAACCGAG 
TTTTGCTACA ACACTTCCGG ACTACCGACA TCACCGCTAG CAACTAGCTG TAACTTTAGG 
ATTAGTTCAT CCAACATTGG AAAGAATCAA AACTGTGATT TTACGACGGC GAGCAGCGCG 
GGCAACCGCT CGTCGTGTGA CAATTCGCCG ATTTTCCCCT CGAGCCCGCA GATGCATCCC 
ATTCACGGTG GCACGGCGTC TACGATGGAT TTCAACAACT GTGCCATCTC GCCGACGCTG 
TCCACCTCGG TTTCGTCGAC GTCCTCGGTG TACCAGACGA CGTACAATAA CTCATCGACG 
ACGGCCGATT CGATGATCCT CAACACTACG GCGGCGAGCA ACGACTTGGG CCCGCCCCAC 
TCACCGCACG TTAACGTACC AAATACACAC AATATTCCTC CTCCTATGCC ACCTCCTCTA 
CCTCCTAGGG TTAAACGAAA AGAGTCCTCC GGGGAGTCGT CACAATCTTC GCAGGTGCGG 
CAAGCACCGG ATGCACCCAA GCTTCCTCCG AGAGATATTA GTCCACCTCC GGTACCACCA 
CGTATCCACA ATCCGTCGCA TCAGATATGC GGGAATGTCC AGCAACAGCA TCAGCTGTTA 
CACAGCGTAG GAGCTTGTGA CAGCTGCAAC CTCGTTAATG TATTACCGAA GGACGAATCT 
CCGTCTTCAT CATCCTCGTC GTCGACGCTG ACCCGCGACA ACTTGAACCA GCACAACATG 
AGCGAACAGC GGCTACTCAT GCTACCGCAT ACCAGCACGA TCATGATGCG ACGAAACTCC 
GCCATGGATC GGGCATCGAA GGAGAAAGTG TCCAACATTA CCGCATCGCC GTCGCTATCC 
GGACTTTCCA GTACAGTGGG GGGTGTGGTC GGCGGTGGAA GTACGGCGAG TAGTGGACCA 
GTCAGCTTAT CCTCATCTAG CGCACCACCA CCGCCATGTG GAGCGAAGAA CAAATCAGTT 
CAAAACTCAC CCGTATCGCA ACAGCAGCAG CAACAACCCG CAGTTTGCGG TCGACGGCCT 
AGTACGAATA TATCGCCGAG ATTTTCACCC GGAGAAACCA CACCTAAATT ACCTCCGAAG 
CCAAAGCAAA CCAATCTGTC ATCACATTCA GATCGAACAA TGTTCCCCTA TCCGAGCACA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021701326.1
CDS	826..6171
Translation	MFAGGQHVSITDATDYDFEKAENAAKWKGVFITSLRKVLEQVHPSLQAREDALLYVESLCLRLLAMLCAKPPPHTVLDVEERIGRTFPTPIDKWALSEARETIDKSKKKKSVLPVDRVHTLLQKEVLQYKIDSSVSLFLVAVLEYISADILKLAGNYVKNIRHIEISREDIEVAMCADKVLMDMFYQGDNSSSMAPSPLPPTPRTSLSYEEVVKELIHDEKQYQRDLHMIIRVFREELVKIVKDPKELDPIFSNIMDIYEVSVTLLGSLEDVIEMSQEQTPPCIGSCFEELAEAAEFDVYAKYAKDITSVQAKEALANLLSRPEANSLMSAGHGFREAVKFYLPKLLLGPIGHAQLYLDYIKILLQLSPSQEDKESFEQVQGLLKPLQCELQGISSLLPKEYFARVNSRARRQSAIEKTRDLQNSVEHWDKDVGQCCNEFIREDTLAKLSSGRRQTERKVFLFDGLLVLCKTRRQIVPGTNYDYRLKERFFMRKVEIIDRPDTEELKYAFEISPREPTSVVLIAKNAQHKNDWMADLVMLNTKSMLERILDSILLDIEKKHPLKVPSPDIYAFAVPDSPSNIVLEEREGTGVPLIKGATLTKLIERLTYHIYADPMFVRTFLTTYRSFCSPTELLRLLVERFDIPDPALVYDSASDKDLGPDTDKFHKNSQREDWKRYKKEYVQPVQFRVLNVLRHWVDHHFYDFERDSELLEELLKFLETVRGKSMRKWVDSVLKIVQRKTESEDSHRQITFAFGHSPPAIEHHLPLTIGEEEYNLLMLHPLELARQLTLLEFEMYKNVKPSELVGSVWTGKDKETTSPNLLRIMHHTTNFTRWIEKSIIEAENFDERVAMASRAIEVMMVLQDLNNFNGVLSIVSAFQGAAVHRLKLTLEEIPKSYQRVLAECRELNNSHFKKYQEKLRSINPPCVPFFGMYLTNILHIEEGNPDFLPKTKLINFSKRRRVAEITGEIQQYQNQPYCLKVDPSIRHFLENLDPFTGMSVTEIQNYLYEESKRIEPKNCRQPLKFARKWPDISLKSPGIKPSSRRHNSSANSSLNLTGTLPFTNKAPLGNSNDTGAEQQSPTASSSSSSVLDYSIFAPVNIHSSSSSQHHQYHHHQQQMYNYQHHSQGSMQYYPGHHQSGGGQQQNHHHYNHHGHQHNLSQSVVNLASTADHQMAPEIPRRSDSIILTPSLSSQVQYNLNNSSLSESSSNTGGGSKLVPSPRYPSSSMSALSSSSSGISVSSFYGIMSASNSDGNSNNNPTTASFKHTPLLSQNKVVANTCAEDSPHSILSASGVGGGGSLSMSSSSATNAASIVSLSEGSAVPQPNTGTAISPCTTMSSPSASSSSTVTAVGTTVTASPAPASSGAADIPPAISPRTDKPVYPPGPPPLRNHGGGATEFCYNTSGLPTSPLATSCNFRISSSNIGKNQNCDFTTASSAGNRSSCDNSPIFPSSPQMHPIHGGTASTMDFNNCAISPTLSTSVSSTSSVYQTTYNNSSTTADSMILNTTAASNDLGPPHSPHVNVPNTHNIPPPMPPPLPPRVKRKESSGESSQSSQVRQAPDAPKLPPRDISPPPVPPRIHNPSHQICGNVQQQHQLLHSVGACDSCNLVNVLPKDESPSSSSSSSTLTRDNLNQHNMSEQRLLMLPHTSTIMMRRNSAMDRASKEKVSNITASPSLSGLSSTVGGVVGGGSTASSGPVSLSSSSAPPPPCGAKNKSVQNSPVSQQQQQQPAVCGRRPSTNISPRFSPGETTPKLPPKPKQTNLSSHSDRTMFPYPSTN

Target ORF information:

RefSeq Version	XM_021845634.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021845634.1

ORF Insert Sequence:

ATGTTCGCTG GCGGCCAGCA TGTATCGATC ACGGATGCGA CAGATTACGA CTTTGAGAAA 
GCGGAAAATG CAGCCAAATG GAAAGGTGTT TTCATAACAT CGCTCAGAAA GGTATTGGAG 
CAAGTGCATC CGTCGTTGCA AGCCCGCGAG GATGCTCTGC TCTACGTCGA AAGTTTATGC 
TTGCGTCTGT TAGCGATGCT GTGTGCAAAA CCACCACCGC ATACAGTATT GGACGTCGAA 
GAACGAATTG GGCGTACGTT TCCTACGCCC ATTGACAAAT GGGCCCTGAG CGAGGCACGT 
GAGACAATAG ACAAATCCAA AAAGAAGAAA TCGGTGCTAC CGGTTGACCG AGTCCATACC 
TTGCTGCAAA AGGAGGTCCT GCAATACAAA ATCGATAGTT CGGTTTCATT GTTCCTGGTG 
GCAGTGCTGG AGTACATTTC GGCGGACATC CTGAAACTAG CTGGCAACTA TGTCAAGAAC 
ATTCGCCACA TCGAGATATC CCGGGAGGAT ATCGAGGTCG CCATGTGCGC TGACAAGGTG 
CTCATGGACA TGTTCTACCA GGGTGACAAC TCGAGCTCGA TGGCACCGAG CCCGTTGCCG 
CCGACGCCTC GTACCAGTCT CAGCTACGAA GAGGTGGTCA AAGAGCTCAT TCACGACGAG 
AAGCAGTACC AGCGGGACCT GCACATGATC ATAAGGGTGT TTCGCGAGGA GCTGGTCAAA 
ATCGTCAAGG ACCCCAAGGA ACTTGACCCT ATCTTCTCGA ACATTATGGA TATCTATGAA 
GTGTCTGTAA CGTTACTGGG TTCACTCGAA GATGTGATCG AGATGTCGCA GGAGCAAACT 
CCACCATGCA TCGGAAGCTG TTTCGAAGAA CTAGCGGAAG CGGCAGAGTT TGATGTGTAC 
GCCAAGTATG CGAAAGATAT CACGTCAGTT CAGGCAAAGG AAGCCTTAGC AAATTTATTG 
TCTAGGCCAG AGGCGAATTC ACTGATGTCG GCTGGTCACG GTTTCAGGGA AGCGGTTAAG 
TTCTATCTAC CAAAACTACT TTTAGGGCCT ATCGGGCATG CACAGTTGTA TTTAGATTAT 
ATTAAAATTT TACTACAGCT TAGTCCCTCG CAGGAGGATA AGGAAAGCTT CGAGCAGGTT 
CAGGGTTTGT TAAAACCCTT GCAGTGCGAG TTGCAAGGCA TATCCTCACT GCTGCCTAAG 
GAATATTTCG CTAGGGTAAA TAGTCGAGCT AGACGTCAGT CGGCAATTGA GAAGACTCGC 
GACTTACAAA ACAGTGTAGA ACATTGGGAC AAGGACGTGG GTCAGTGTTG CAACGAGTTC 
ATTCGGGAGG ATACCCTTGC CAAGCTCAGT TCCGGCAGGC GGCAAACCGA ACGGAAGGTG 
TTCCTATTTG ATGGGTTGTT GGTGTTGTGT AAAACCCGAC GACAGATTGT GCCTGGCACC 
AACTACGATT ACCGATTGAA GGAACGCTTT TTCATGCGAA AGGTGGAGAT CATCGATCGG 
CCTGACACCG AGGAGCTCAA GTATGCTTTC GAGATCTCAC CCCGGGAGCC GACTAGCGTT 
GTACTTATTG CTAAAAATGC GCAACACAAA AACGACTGGA TGGCTGATTT GGTTATGCTG 
AACACGAAAT CCATGTTGGA ACGCATTCTA GACAGTATTC TCCTAGATAT TGAAAAGAAA 
CATCCACTCA AAGTACCTAG TCCGGACATA TATGCATTTG CCGTGCCTGA TTCGCCCTCG 
AATATAGTGC TGGAAGAGCG AGAAGGCACG GGAGTTCCAC TGATCAAGGG CGCCACTCTA 
ACGAAACTGA TCGAACGGCT TACCTATCAC ATTTACGCCG ATCCAATGTT CGTGCGTACC 
TTCCTAACAA CGTACCGATC GTTTTGTTCG CCCACAGAGC TTCTTAGGTT ACTGGTGGAA 
CGGTTTGACA TACCGGATCC GGCACTAGTG TACGACAGTG CCAGCGACAA GGATCTAGGG 
CCAGACACGG ACAAGTTTCA TAAAAATTCA CAACGGGAAG ACTGGAAACG CTATAAGAAG 
GAGTATGTCC AACCGGTACA GTTCCGGGTA CTGAACGTTC TTCGGCACTG GGTAGACCAT 
CATTTTTACG ACTTTGAACG TGACTCCGAG CTTCTGGAAG AGTTGTTAAA GTTCCTTGAA 
ACGGTACGCG GTAAATCGAT GCGAAAGTGG GTTGACTCAG TGCTGAAGAT TGTGCAACGG 
AAGACCGAAA GCGAAGATAG TCATCGGCAG ATAACGTTTG CCTTCGGCCA TAGTCCACCG 
GCGATCGAGC ATCACCTACC GCTGACTATT GGTGAGGAGG AATATAACCT GCTGATGTTG 
CATCCCCTGG AACTTGCCCG TCAGCTAACG TTGTTAGAAT TTGAAATGTA TAAAAATGTA 
AAACCATCGG AACTGGTCGG TTCGGTGTGG ACCGGGAAAG ATAAAGAAAC TACTAGTCCT 
AATTTATTAA GAATTATGCA TCACACTACG AATTTCACCC GCTGGATTGA GAAATCAATT 
ATCGAGGCGG AGAATTTCGA CGAACGTGTG GCAATGGCAT CACGTGCAAT AGAGGTGATG 
ATGGTTCTGC AGGATCTAAA CAACTTCAAC GGTGTGCTGT CGATCGTGTC GGCCTTCCAG 
GGGGCGGCCG TCCACCGGCT CAAGCTAACG CTGGAGGAAA TCCCCAAGAG CTACCAGCGG 
GTGCTGGCAG AGTGTCGGGA GCTGAACAAT TCGCACTTCA AAAAGTATCA GGAAAAGCTG 
CGTTCAATAA ATCCACCTTG TGTGCCATTT TTTGGAATGT ATTTAACCAA TATTTTACAT 
ATAGAGGAAG GGAATCCAGA TTTTTTACCA AAGACGAAAC TAATTAACTT CTCTAAGAGA 
AGACGTGTAG CTGAAATCAC TGGTGAAATA CAGCAATATC AAAATCAACC ATACTGTTTG 
AAAGTTGACC CTAGCATTAG GCATTTTCTA GAAAATTTAG ATCCTTTTAC AGGCATGAGT 
GTAACCGAGA TCCAAAACTA TCTCTACGAG GAAAGTAAAA GGATAGAGCC GAAAAATTGT 
CGCCAACCGT TAAAATTCGC ACGCAAGTGG CCAGATATTT CACTAAAGTC TCCCGGCATC 
AAGCCTTCTT CGAGACGACA CAACAGTTCG GCCAACTCGT CATTGAATCT AACGGGTACG 
TTGCCATTTA CGAACAAAGC GCCGCTTGGG AACAGCAACG ATACCGGTGC GGAACAGCAG 
TCACCAACAG CCAGTTCATC GTCGTCTTCC GTGCTCGACT ATTCGATATT TGCTCCTGTG 
AACATTCACT CTTCGTCGTC ATCACAACAC CATCAATACC ATCACCATCA GCAACAAATG 
TACAACTATC AGCATCATTC GCAGGGATCG ATGCAATATT ATCCGGGCCA CCATCAGTCG 
GGTGGTGGCC AGCAGCAAAA CCATCACCAT TATAATCATC ACGGGCATCA GCACAACTTG 
TCCCAGTCAG TGGTCAATCT CGCATCAACG GCAGACCATC AGATGGCGCC GGAGATTCCA 
CGACGTTCCG ATAGTATTAT CCTTACCCCT TCACTATCGT CCCAAGTACA GTACAACCTA 
AACAATAGTA GCTTATCCGA ATCCAGCAGT AATACCGGTG GTGGCAGTAA ACTCGTTCCA 
AGCCCTCGCT ATCCATCGTC ATCAATGAGT GCGCTTTCGT CTTCGTCCTC CGGCATAAGT 
GTTTCTTCGT TCTATGGAAT CATGAGCGCA TCGAACAGTG ATGGTAACAG CAACAATAAT 
CCTACTACTG CTAGCTTTAA ACATACGCCA TTGCTTAGTC AGAACAAAGT AGTTGCCAAT 
ACGTGTGCTG AAGACAGTCC CCATTCGATT CTTTCAGCGT CTGGTGTAGG GGGCGGAGGA 
AGTCTCAGCA TGAGCAGCTC GTCTGCAACC AACGCCGCTA GTATTGTTTC GCTAAGTGAG 
GGTTCAGCCG TTCCTCAACC CAACACCGGT ACAGCCATCT CGCCGTGTAC GACGATGTCT 
TCGCCATCGG CCAGCTCGTC CTCGACCGTG ACTGCCGTGG GAACAACGGT TACTGCATCA 
CCAGCACCAG CATCGTCCGG TGCTGCCGAC ATTCCACCTG CGATTAGTCC ACGCACTGAC 
AAGCCGGTAT ACCCGCCGGG ACCGCCACCC TTACGGAACC ATGGAGGTGG GGCAACCGAG 
TTTTGCTACA ACACTTCCGG ACTACCGACA TCACCGCTAG CAACTAGCTG TAACTTTAGG 
ATTAGTTCAT CCAACATTGG AAAGAATCAA AACTGTGATT TTACGACGGC GAGCAGCGCG 
GGCAACCGCT CGTCGTGTGA CAATTCGCCG ATTTTCCCCT CGAGCCCGCA GATGCATCCC 
ATTCACGGTG GCACGGCGTC TACGATGGAT TTCAACAACT GTGCCATCTC GCCGACGCTG 
TCCACCTCGG TTTCGTCGAC GTCCTCGGTG TACCAGACGA CGTACAATAA CTCATCGACG 
ACGGCCGATT CGATGATCCT CAACACTACG GCGGCGAGCA ACGACTTGGG CCCGCCCCAC 
TCACCGCACG TTAACGTACC AAATACACAC AATATTCCTC CTCCTATGCC ACCTCCTCTA 
CCTCCTAGGG TTAAACGAAA AGAGTCCTCC GGGGAGTCGT CACAATCTTC GCAGGTGCGG 
CAAGCACCGG ATGCACCCAA GCTTCCTCCG AGAGATATTA GTCCACCTCC GGTACCACCA 
CGTATCCACA ATCCGTCGCA TCAGATATGC GGGAATGTCC AGCAACAGCA TCAGCTGTTA 
CACAGCGTAG GAGCTTGTGA CAGCTGCAAC CTCGTTAATG TATTACCGAA GGACGAATCT 
CCGTCTTCAT CATCCTCGTC GTCGACGCTG ACCCGCGACA ACTTGAACCA GCACAACATG 
AGCGAACAGC GGCTACTCAT GCTACCGCAT ACCAGCACGA TCATGATGCG ACGAAACTCC 
GCCATGGATC GGGCATCGAA GGAGAAAGTG TCCAACATTA CCGCATCGCC GTCGCTATCC 
GGACTTTCCA GTACAGTGGG GGGTGTGGTC GGCGGTGGAA GTACGGCGAG TAGTGGACCA 
GTCAGCTTAT CCTCATCTAG CGCACCACCA CCGCCATGTG GAGCGAAGAA CAAATCAGTT 
CAAAACTCAC CCGTATCGCA ACAGCAGCAG CAACAACCCG CAGTTTGCGG TCGACGGCCT 
AGTACGAATA TATCGCCGAG ATTTTCACCC GGAGAAACCA CACCTAAATT ACCTCCGAAG 
CCAAAGCAAA CCAATCTGTC ATCACATTCA GATCGAACAA TGTTCCCCTA TCCGAGCACA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568816 ( XM_021845635.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021701327.1 Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X3, mRNA.

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CloneID	OAe122686
Clone ID Related Accession (Same CDS sequence)	XM_021845635.1
Accession Version	XM_021845635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	protein son of sevenless isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGCTG GCGGCCAGCA TGTATCGATC ACGGATGCGA CAGATTACGA CTTTGAGAAA 
GCGGAAAATG CAGCCAAATG GAAAGGTGTT TTCATAACAT CGCTCAGAAA GGTATTGGAG 
CAAGTGCATC CGTCGTTGCA AGCCCGCGAG GATGCTCTGC TCTACGTCGA AAGTTTATGC 
TTGCGTCTGT TAGCGATGCT GTGTGCAAAA CCACCACCGC ATACAGTATT GGACGTCGAA 
GAACGAATTG GGCGTACGTT TCCTACGCCC ATTGACAAAT GGGCCCTGAG CGAGGCACGT 
GAGACAATAG ACAAATCCAA AAAGAAGAAA TCGGTGCTAC CGGTTGACCG AGTCCATACC 
TTGCTGCAAA AGGAGGTCCT GCAATACAAA ATCGATAGTT CGGTTTCATT GTTCCTGGTG 
GCAGTGCTGG AGTACATTTC GGCGGACATC CTGAAACTAG CTGGCAACTA TGTCAAGAAC 
ATTCGCCACA TCGAGATATC CCGGGAGGAT ATCGAGGTCG CCATGTGCGC TGACAAGGTG 
CTCATGGACA TGTTCTACCA GGGTGACAAC TCGAGCTCGA TGGCACCGAG CCCGTTGCCG 
CCGACGCCTC GTACCAGTCT CAGCTACGAA GAGGTGGTCA AAGAGCTCAT TCACGACGAG 
AAGCAGTACC AGCGGGACCT GCACATGATC ATAAGGGTGT TTCGCGAGGA GCTGGTCAAA 
ATCGTCAAGG ACCCCAAGGA ACTTGACCCT ATCTTCTCGA ACATTATGGA TATCTATGAA 
GTGTCTGTAA CGTTACTGGG TTCACTCGAA GATGTGATCG AGATGTCGCA GGAGCAAACT 
CCACCATGCA TCGGAAGCTG TTTCGAAGAA CTAGCGGAAG CGGCAGAGTT TGATGTGTAC 
GCCAAGTATG CGAAAGATAT CACGTCAGTT CAGGCAAAGG AAGCCTTAGC AAATTTATTG 
TCTAGGCCAG AGGTACTAGA TAGCAATAGT TTCAGAATTC ATAATCGTCA TCCTAACCCA 
TGCGATCTAT TTCAGGCGAA TTCACTGATG TCGGCTGGTC ACGGTTTCAG GGAAGCGGTT 
AAGTTCTATC TACCAAAACT ACTTTTAGGG CCTATCGGGC ATGCACAGTT GTATTTAGAT 
TATATTAAAA TTTTACTACA GCTTAGTCCC TCGCAGGAGG ATAAGGAAAG CTTCGAGCAG 
GTTCAGGGTT TGTTAAAACC CTTGCAGTGC GAGTTGCAAG GCATATCCTC ACTGCTGCCT 
AAGGAATATT TCGCTAGGGT AAATAGTCGA GCTAGACGTC AGTCGGCAAT TGAGAAGACT 
CGCGACTTAC AAAACAGTGT AGAACATTGG GACAAGGACG TGGGTCAGTG TTGCAACGAG 
TTCATTCGGG AGGATACCCT TGCCAAGCTC AGTTCCGGCA GGCGGCAAAC CGAACGGAAG 
GTGTTCCTAT TTGATGGGTT GTTGGTGTTG TGTAAAACCC GACGACAGAT TGTGCCTGGC 
ACCAACTACG ATTACCGATT GAAGGAACGC TTTTTCATGC GAAAGGTGGA GATCATCGAT 
CGGCCTGACA CCGAGGAGCT CAAGTATGCT TTCGAGATCT CACCCCGGGA GCCGACTAGC 
GTTGTACTTA TTGCTAAAAA TGCGCAACAC AAAAACGACT GGATGGCTGA TTTGGTTATG 
CTGAACACGA AATCCATGTT GGAACGCATT CTAGACAGTA TTCTCCTAGA TATTGAAAAG 
AAACATCCAC TCAAAGTACC TAGTCCGGAC ATATATGCAT TTGCCGTGCC TGATTCGCCC 
TCGAATATAG TGCTGGAAGA GCGAGAAGGC ACGGGAGTTC CACTGATCAA GGGCGCCACT 
CTAACGAAAC TGATCGAACG GCTTACCTAT CACATTTACG CCGATCCAAT GTTCGTGCGT 
ACCTTCCTAA CAACGTACCG ATCGTTTTGT TCGCCCACAG AGCTTCTTAG GTTACTGGTG 
GAACGGTTTG ACATACCGGA TCCGGCACTA GTGTACGACA GTGCCAGCGA CAAGGATCTA 
GGGCCAGACA CGGACAAGTT TCATAAAAAT TCACAACGGG AAGACTGGAA ACGCTATAAG 
AAGGAGTATG TCCAACCGGT ACAGTTCCGG GTACTGAACG TTCTTCGGCA CTGGGTAGAC 
CATCATTTTT ACGACTTTGA ACGTGACTCC GAGCTTCTGG AAGAGTTGTT AAAGTTCCTT 
GAAACGGTAC GCGGTAAATC GATGCGAAAG TGGGTTGACT CAGTGCTGAA GATTGTGCAA 
CGGAAGACCG AAAGCGAAGA TAGTCATCGG CAGATAACGT TTGCCTTCGG CCATAGTCCA 
CCGGCGATCG AGCATCACCT ACCGCTGACT ATTGGTGAGG AGGAATATAA CCTGCTGATG 
TTGCATCCCC TGGAACTTGC CCGTCAGCTA ACGTTGTTAG AATTTGAAAT GTATAAAAAT 
GTAAAACCAT CGGAACTGGT CGGTTCGGTG TGGACCGGGA AAGATAAAGA AACTACTAGT 
CCTAATTTAT TAAGAATTAT GCATCACACT ACGAATTTCA CCCGCTGGAT TGAGAAATCA 
ATTATCGAGG CGGAGAATTT CGACGAACGT GTGGCAATGG CATCACGTGC AATAGAGGTG 
ATGATGGTTC TGCAGGATCT AAACAACTTC AACGGTGTGC TGTCGATCGT GTCGGCCTTC 
CAGGGGGCGG CCGTCCACCG GCTCAAGCTA ACGCTGGAGG AAATCCCCAA GAGCTACCAG 
CGGGTGCTGG CAGAGTGTCG GGAGCTGAAC AATTCGCACT TCAAAAAGTA TCAGGAAAAG 
CTGCGTTCAA TAAATCCACC TTGTGTGCCA TTTTTTGGAA TGTATTTAAC CAATATTTTA 
CATATAGAGG AAGGGAATCC AGATTTTTTA CCAAAGACGA AACTAATTAA CTTCTCTAAG 
AGAAGACGTG TAGCTGAAAT CACTGGTGAA ATACAGCAAT ATCAAAATCA ACCATACTGT 
TTGAAAGTTG ACCCTAGCAT TAGGCATTTT CTAGAAAATT TAGATCCTTT TACAGGCATG 
AGTGTAACCG AGATCCAAAA CTATCTCTAC GAGGAAAGTA AAAGGATAGA GCCGAAAAAT 
TGTCGCCAAC CGTTAAAATT CGCACGCAAG TGGCCAGATA TTTCACTAAA GTCTCCCGGC 
ATCAAGCCTT CTTCGAGACG ACACAACAGT TCGGCCAACT CGTCATTGAA TCTAACGGGT 
ACGTTGCCAT TTACGAACAA AGCGCCGCTT GGGAACAGCA ACGATACCGG TGCGGAACAG 
CAGTCACCAA CAGCCAGTTC ATCGTCGTCT TCCGTGCTCG ACTATTCGAT ATTTGCTCCT 
GTGAACATTC ACTCTTCGTC GTCATCACAA CACCATCAAT ACCATCACCA TCAGCAACAA 
ATGTACAACT ATCAGCATCA TTCGCAGGGA TCGATGCAAT ATTATCCGGG CCACCATCAG 
TCGGGTGGTG GCCAGCAGCA AAACCATCAC CATTATAATC ATCACGGGCA TCAGCACAAC 
TTGTCCCAGT CAGTGGTCAA TCTCGCATCA ACGGCAGACC ATCAGATGGC GCCGGAGATT 
CCACGACGTT CCGATAGTAT TATCCTTACC CCTTCACTAT CGTCCCAAGT ACAGTACAAC 
CTAAACAATA GTAGCTTATC CGAATCCAGC AGTAATACCG GTGGTGGCAG TAAACTCGTT 
CCAAGCCCTC GCTATCCATC GTCATCAATG AGTGCGCTTT CGTCTTCGTC CTCCGGCATA 
AGTGTTTCTT CGTTCTATGG AATCATGAGC GCATCGAACA GTGATGCGTC TGGTGTAGGG 
GGCGGAGGAA GTCTCAGCAT GAGCAGCTCG TCTGCAACCA ACGCCGCTAG TATTGTTTCG 
CTAAGTGAGG GTTCAGCCGT TCCTCAACCC AACACCGGTA CAGCCATCTC GCCGTGTACG 
ACGATGTCTT CGCCATCGGC CAGCTCGTCC TCGACCGTGA CTGCCGTGGG AACAACGGTT 
ACTGCATCAC CAGCACCAGC ATCGTCCGGT GCTGCCGACA TTCCACCTGC GATTAGTCCA 
CGCACTGACA AGCCGGTATA CCCGCCGGGA CCGCCACCCT TACGGAACCA TGGAGGTGGG 
GCAACCGAGT TTTGCTACAA CACTTCCGGA CTACCGACAT CACCGCTAGC AACTAGCTGT 
AACTTTAGGA TTAGTTCATC CAACATTGGA AAGAATCAAA ACTGTGATTT TACGACGGCG 
AGCAGCGCGG GCAACCGCTC GTCGTGTGAC AATTCGCCGA TTTTCCCCTC GAGCCCGCAG 
ATGCATCCCA TTCACGGTGG CACGGCGTCT ACGATGGATT TCAACAACTG TGCCATCTCG 
CCGACGCTGT CCACCTCGGT TTCGTCGACG TCCTCGGTGT ACCAGACGAC GTACAATAAC 
TCATCGACGA CGGCCGATTC GATGATCCTC AACACTACGG CGGCGAGCAA CGACTTGGGC 
CCGCCCCACT CACCGCACGT TAACGTACCA AATACACACA ATATTCCTCC TCCTATGCCA 
CCTCCTCTAC CTCCTAGGGT TAAACGAAAA GAGTCCTCCG GGGAGTCGTC ACAATCTTCG 
CAGGTGCGGC AAGCACCGGA TGCACCCAAG CTTCCTCCGA GAGATATTAG TCCACCTCCG 
GTACCACCAC GTATCCACAA TCCGTCGCAT CAGATATGCG GGAATGTCCA GCAACAGCAT 
CAGCTGTTAC ACAGCGTAGG AGCTTGTGAC AGCTGCAACC TCGTTAATGT ATTACCGAAG 
GACGAATCTC CGTCTTCATC ATCCTCGTCG TCGACGCTGA CCCGCGACAA CTTGAACCAG 
CACAACATGA GCGAACAGCG GCTACTCATG CTACCGCATA CCAGCACGAT CATGATGCGA 
CGAAACTCCG CCATGGATCG GGCATCGAAG GAGAAAGTGT CCAACATTAC CGCATCGCCG 
TCGCTATCCG GACTTTCCAG TACAGTGGGG GGTGTGGTCG GCGGTGGAAG TACGGCGAGT 
AGTGGACCAG TCAGCTTATC CTCATCTAGC GCACCACCAC CGCCATGTGG AGCGAAGAAC 
AAATCAGTTC AAAACTCACC CGTATCGCAA CAGCAGCAGC AACAACCCGC AGTTTGCGGT 
CGACGGCCTA GTACGAATAT ATCGCCGAGA TTTTCACCCG GAGAAACCAC ACCTAAATTA 
CCTCCGAAGC CAAAGCAAAC CAATCTGTCA TCACATTCAG ATCGAACAAT GTTCCCCTAT 
CCGAGCACAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021701327.1
CDS	826..6120
Translation	MFAGGQHVSITDATDYDFEKAENAAKWKGVFITSLRKVLEQVHPSLQAREDALLYVESLCLRLLAMLCAKPPPHTVLDVEERIGRTFPTPIDKWALSEARETIDKSKKKKSVLPVDRVHTLLQKEVLQYKIDSSVSLFLVAVLEYISADILKLAGNYVKNIRHIEISREDIEVAMCADKVLMDMFYQGDNSSSMAPSPLPPTPRTSLSYEEVVKELIHDEKQYQRDLHMIIRVFREELVKIVKDPKELDPIFSNIMDIYEVSVTLLGSLEDVIEMSQEQTPPCIGSCFEELAEAAEFDVYAKYAKDITSVQAKEALANLLSRPEVLDSNSFRIHNRHPNPCDLFQANSLMSAGHGFREAVKFYLPKLLLGPIGHAQLYLDYIKILLQLSPSQEDKESFEQVQGLLKPLQCELQGISSLLPKEYFARVNSRARRQSAIEKTRDLQNSVEHWDKDVGQCCNEFIREDTLAKLSSGRRQTERKVFLFDGLLVLCKTRRQIVPGTNYDYRLKERFFMRKVEIIDRPDTEELKYAFEISPREPTSVVLIAKNAQHKNDWMADLVMLNTKSMLERILDSILLDIEKKHPLKVPSPDIYAFAVPDSPSNIVLEEREGTGVPLIKGATLTKLIERLTYHIYADPMFVRTFLTTYRSFCSPTELLRLLVERFDIPDPALVYDSASDKDLGPDTDKFHKNSQREDWKRYKKEYVQPVQFRVLNVLRHWVDHHFYDFERDSELLEELLKFLETVRGKSMRKWVDSVLKIVQRKTESEDSHRQITFAFGHSPPAIEHHLPLTIGEEEYNLLMLHPLELARQLTLLEFEMYKNVKPSELVGSVWTGKDKETTSPNLLRIMHHTTNFTRWIEKSIIEAENFDERVAMASRAIEVMMVLQDLNNFNGVLSIVSAFQGAAVHRLKLTLEEIPKSYQRVLAECRELNNSHFKKYQEKLRSINPPCVPFFGMYLTNILHIEEGNPDFLPKTKLINFSKRRRVAEITGEIQQYQNQPYCLKVDPSIRHFLENLDPFTGMSVTEIQNYLYEESKRIEPKNCRQPLKFARKWPDISLKSPGIKPSSRRHNSSANSSLNLTGTLPFTNKAPLGNSNDTGAEQQSPTASSSSSSVLDYSIFAPVNIHSSSSSQHHQYHHHQQQMYNYQHHSQGSMQYYPGHHQSGGGQQQNHHHYNHHGHQHNLSQSVVNLASTADHQMAPEIPRRSDSIILTPSLSSQVQYNLNNSSLSESSSNTGGGSKLVPSPRYPSSSMSALSSSSSGISVSSFYGIMSASNSDASGVGGGGSLSMSSSSATNAASIVSLSEGSAVPQPNTGTAISPCTTMSSPSASSSSTVTAVGTTVTASPAPASSGAADIPPAISPRTDKPVYPPGPPPLRNHGGGATEFCYNTSGLPTSPLATSCNFRISSSNIGKNQNCDFTTASSAGNRSSCDNSPIFPSSPQMHPIHGGTASTMDFNNCAISPTLSTSVSSTSSVYQTTYNNSSTTADSMILNTTAASNDLGPPHSPHVNVPNTHNIPPPMPPPLPPRVKRKESSGESSQSSQVRQAPDAPKLPPRDISPPPVPPRIHNPSHQICGNVQQQHQLLHSVGACDSCNLVNVLPKDESPSSSSSSSTLTRDNLNQHNMSEQRLLMLPHTSTIMMRRNSAMDRASKEKVSNITASPSLSGLSSTVGGVVGGGSTASSGPVSLSSSSAPPPPCGAKNKSVQNSPVSQQQQQQPAVCGRRPSTNISPRFSPGETTPKLPPKPKQTNLSSHSDRTMFPYPSTN

Target ORF information:

RefSeq Version	XM_021845635.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021845635.1

ORF Insert Sequence:

ATGTTCGCTG GCGGCCAGCA TGTATCGATC ACGGATGCGA CAGATTACGA CTTTGAGAAA 
GCGGAAAATG CAGCCAAATG GAAAGGTGTT TTCATAACAT CGCTCAGAAA GGTATTGGAG 
CAAGTGCATC CGTCGTTGCA AGCCCGCGAG GATGCTCTGC TCTACGTCGA AAGTTTATGC 
TTGCGTCTGT TAGCGATGCT GTGTGCAAAA CCACCACCGC ATACAGTATT GGACGTCGAA 
GAACGAATTG GGCGTACGTT TCCTACGCCC ATTGACAAAT GGGCCCTGAG CGAGGCACGT 
GAGACAATAG ACAAATCCAA AAAGAAGAAA TCGGTGCTAC CGGTTGACCG AGTCCATACC 
TTGCTGCAAA AGGAGGTCCT GCAATACAAA ATCGATAGTT CGGTTTCATT GTTCCTGGTG 
GCAGTGCTGG AGTACATTTC GGCGGACATC CTGAAACTAG CTGGCAACTA TGTCAAGAAC 
ATTCGCCACA TCGAGATATC CCGGGAGGAT ATCGAGGTCG CCATGTGCGC TGACAAGGTG 
CTCATGGACA TGTTCTACCA GGGTGACAAC TCGAGCTCGA TGGCACCGAG CCCGTTGCCG 
CCGACGCCTC GTACCAGTCT CAGCTACGAA GAGGTGGTCA AAGAGCTCAT TCACGACGAG 
AAGCAGTACC AGCGGGACCT GCACATGATC ATAAGGGTGT TTCGCGAGGA GCTGGTCAAA 
ATCGTCAAGG ACCCCAAGGA ACTTGACCCT ATCTTCTCGA ACATTATGGA TATCTATGAA 
GTGTCTGTAA CGTTACTGGG TTCACTCGAA GATGTGATCG AGATGTCGCA GGAGCAAACT 
CCACCATGCA TCGGAAGCTG TTTCGAAGAA CTAGCGGAAG CGGCAGAGTT TGATGTGTAC 
GCCAAGTATG CGAAAGATAT CACGTCAGTT CAGGCAAAGG AAGCCTTAGC AAATTTATTG 
TCTAGGCCAG AGGTACTAGA TAGCAATAGT TTCAGAATTC ATAATCGTCA TCCTAACCCA 
TGCGATCTAT TTCAGGCGAA TTCACTGATG TCGGCTGGTC ACGGTTTCAG GGAAGCGGTT 
AAGTTCTATC TACCAAAACT ACTTTTAGGG CCTATCGGGC ATGCACAGTT GTATTTAGAT 
TATATTAAAA TTTTACTACA GCTTAGTCCC TCGCAGGAGG ATAAGGAAAG CTTCGAGCAG 
GTTCAGGGTT TGTTAAAACC CTTGCAGTGC GAGTTGCAAG GCATATCCTC ACTGCTGCCT 
AAGGAATATT TCGCTAGGGT AAATAGTCGA GCTAGACGTC AGTCGGCAAT TGAGAAGACT 
CGCGACTTAC AAAACAGTGT AGAACATTGG GACAAGGACG TGGGTCAGTG TTGCAACGAG 
TTCATTCGGG AGGATACCCT TGCCAAGCTC AGTTCCGGCA GGCGGCAAAC CGAACGGAAG 
GTGTTCCTAT TTGATGGGTT GTTGGTGTTG TGTAAAACCC GACGACAGAT TGTGCCTGGC 
ACCAACTACG ATTACCGATT GAAGGAACGC TTTTTCATGC GAAAGGTGGA GATCATCGAT 
CGGCCTGACA CCGAGGAGCT CAAGTATGCT TTCGAGATCT CACCCCGGGA GCCGACTAGC 
GTTGTACTTA TTGCTAAAAA TGCGCAACAC AAAAACGACT GGATGGCTGA TTTGGTTATG 
CTGAACACGA AATCCATGTT GGAACGCATT CTAGACAGTA TTCTCCTAGA TATTGAAAAG 
AAACATCCAC TCAAAGTACC TAGTCCGGAC ATATATGCAT TTGCCGTGCC TGATTCGCCC 
TCGAATATAG TGCTGGAAGA GCGAGAAGGC ACGGGAGTTC CACTGATCAA GGGCGCCACT 
CTAACGAAAC TGATCGAACG GCTTACCTAT CACATTTACG CCGATCCAAT GTTCGTGCGT 
ACCTTCCTAA CAACGTACCG ATCGTTTTGT TCGCCCACAG AGCTTCTTAG GTTACTGGTG 
GAACGGTTTG ACATACCGGA TCCGGCACTA GTGTACGACA GTGCCAGCGA CAAGGATCTA 
GGGCCAGACA CGGACAAGTT TCATAAAAAT TCACAACGGG AAGACTGGAA ACGCTATAAG 
AAGGAGTATG TCCAACCGGT ACAGTTCCGG GTACTGAACG TTCTTCGGCA CTGGGTAGAC 
CATCATTTTT ACGACTTTGA ACGTGACTCC GAGCTTCTGG AAGAGTTGTT AAAGTTCCTT 
GAAACGGTAC GCGGTAAATC GATGCGAAAG TGGGTTGACT CAGTGCTGAA GATTGTGCAA 
CGGAAGACCG AAAGCGAAGA TAGTCATCGG CAGATAACGT TTGCCTTCGG CCATAGTCCA 
CCGGCGATCG AGCATCACCT ACCGCTGACT ATTGGTGAGG AGGAATATAA CCTGCTGATG 
TTGCATCCCC TGGAACTTGC CCGTCAGCTA ACGTTGTTAG AATTTGAAAT GTATAAAAAT 
GTAAAACCAT CGGAACTGGT CGGTTCGGTG TGGACCGGGA AAGATAAAGA AACTACTAGT 
CCTAATTTAT TAAGAATTAT GCATCACACT ACGAATTTCA CCCGCTGGAT TGAGAAATCA 
ATTATCGAGG CGGAGAATTT CGACGAACGT GTGGCAATGG CATCACGTGC AATAGAGGTG 
ATGATGGTTC TGCAGGATCT AAACAACTTC AACGGTGTGC TGTCGATCGT GTCGGCCTTC 
CAGGGGGCGG CCGTCCACCG GCTCAAGCTA ACGCTGGAGG AAATCCCCAA GAGCTACCAG 
CGGGTGCTGG CAGAGTGTCG GGAGCTGAAC AATTCGCACT TCAAAAAGTA TCAGGAAAAG 
CTGCGTTCAA TAAATCCACC TTGTGTGCCA TTTTTTGGAA TGTATTTAAC CAATATTTTA 
CATATAGAGG AAGGGAATCC AGATTTTTTA CCAAAGACGA AACTAATTAA CTTCTCTAAG 
AGAAGACGTG TAGCTGAAAT CACTGGTGAA ATACAGCAAT ATCAAAATCA ACCATACTGT 
TTGAAAGTTG ACCCTAGCAT TAGGCATTTT CTAGAAAATT TAGATCCTTT TACAGGCATG 
AGTGTAACCG AGATCCAAAA CTATCTCTAC GAGGAAAGTA AAAGGATAGA GCCGAAAAAT 
TGTCGCCAAC CGTTAAAATT CGCACGCAAG TGGCCAGATA TTTCACTAAA GTCTCCCGGC 
ATCAAGCCTT CTTCGAGACG ACACAACAGT TCGGCCAACT CGTCATTGAA TCTAACGGGT 
ACGTTGCCAT TTACGAACAA AGCGCCGCTT GGGAACAGCA ACGATACCGG TGCGGAACAG 
CAGTCACCAA CAGCCAGTTC ATCGTCGTCT TCCGTGCTCG ACTATTCGAT ATTTGCTCCT 
GTGAACATTC ACTCTTCGTC GTCATCACAA CACCATCAAT ACCATCACCA TCAGCAACAA 
ATGTACAACT ATCAGCATCA TTCGCAGGGA TCGATGCAAT ATTATCCGGG CCACCATCAG 
TCGGGTGGTG GCCAGCAGCA AAACCATCAC CATTATAATC ATCACGGGCA TCAGCACAAC 
TTGTCCCAGT CAGTGGTCAA TCTCGCATCA ACGGCAGACC ATCAGATGGC GCCGGAGATT 
CCACGACGTT CCGATAGTAT TATCCTTACC CCTTCACTAT CGTCCCAAGT ACAGTACAAC 
CTAAACAATA GTAGCTTATC CGAATCCAGC AGTAATACCG GTGGTGGCAG TAAACTCGTT 
CCAAGCCCTC GCTATCCATC GTCATCAATG AGTGCGCTTT CGTCTTCGTC CTCCGGCATA 
AGTGTTTCTT CGTTCTATGG AATCATGAGC GCATCGAACA GTGATGCGTC TGGTGTAGGG 
GGCGGAGGAA GTCTCAGCAT GAGCAGCTCG TCTGCAACCA ACGCCGCTAG TATTGTTTCG 
CTAAGTGAGG GTTCAGCCGT TCCTCAACCC AACACCGGTA CAGCCATCTC GCCGTGTACG 
ACGATGTCTT CGCCATCGGC CAGCTCGTCC TCGACCGTGA CTGCCGTGGG AACAACGGTT 
ACTGCATCAC CAGCACCAGC ATCGTCCGGT GCTGCCGACA TTCCACCTGC GATTAGTCCA 
CGCACTGACA AGCCGGTATA CCCGCCGGGA CCGCCACCCT TACGGAACCA TGGAGGTGGG 
GCAACCGAGT TTTGCTACAA CACTTCCGGA CTACCGACAT CACCGCTAGC AACTAGCTGT 
AACTTTAGGA TTAGTTCATC CAACATTGGA AAGAATCAAA ACTGTGATTT TACGACGGCG 
AGCAGCGCGG GCAACCGCTC GTCGTGTGAC AATTCGCCGA TTTTCCCCTC GAGCCCGCAG 
ATGCATCCCA TTCACGGTGG CACGGCGTCT ACGATGGATT TCAACAACTG TGCCATCTCG 
CCGACGCTGT CCACCTCGGT TTCGTCGACG TCCTCGGTGT ACCAGACGAC GTACAATAAC 
TCATCGACGA CGGCCGATTC GATGATCCTC AACACTACGG CGGCGAGCAA CGACTTGGGC 
CCGCCCCACT CACCGCACGT TAACGTACCA AATACACACA ATATTCCTCC TCCTATGCCA 
CCTCCTCTAC CTCCTAGGGT TAAACGAAAA GAGTCCTCCG GGGAGTCGTC ACAATCTTCG 
CAGGTGCGGC AAGCACCGGA TGCACCCAAG CTTCCTCCGA GAGATATTAG TCCACCTCCG 
GTACCACCAC GTATCCACAA TCCGTCGCAT CAGATATGCG GGAATGTCCA GCAACAGCAT 
CAGCTGTTAC ACAGCGTAGG AGCTTGTGAC AGCTGCAACC TCGTTAATGT ATTACCGAAG 
GACGAATCTC CGTCTTCATC ATCCTCGTCG TCGACGCTGA CCCGCGACAA CTTGAACCAG 
CACAACATGA GCGAACAGCG GCTACTCATG CTACCGCATA CCAGCACGAT CATGATGCGA 
CGAAACTCCG CCATGGATCG GGCATCGAAG GAGAAAGTGT CCAACATTAC CGCATCGCCG 
TCGCTATCCG GACTTTCCAG TACAGTGGGG GGTGTGGTCG GCGGTGGAAG TACGGCGAGT 
AGTGGACCAG TCAGCTTATC CTCATCTAGC GCACCACCAC CGCCATGTGG AGCGAAGAAC 
AAATCAGTTC AAAACTCACC CGTATCGCAA CAGCAGCAGC AACAACCCGC AGTTTGCGGT 
CGACGGCCTA GTACGAATAT ATCGCCGAGA TTTTCACCCG GAGAAACCAC ACCTAAATTA 
CCTCCGAAGC CAAAGCAAAC CAATCTGTCA TCACATTCAG ATCGAACAAT GTTCCCCTAT 
CCGAGCACAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568816 ( XM_021845636.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021701328.1 Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X4, mRNA.

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CloneID	OAe122687
Clone ID Related Accession (Same CDS sequence)	XM_021845636.1
Accession Version	XM_021845636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	protein son of sevenless isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGCTG GCGGCCAGCA TGTATCGATC ACGGATGCGA CAGATTACGA CTTTGAGAAA 
GCGGAAAATG CAGCCAAATG GAAAGGTGTT TTCATAACAT CGCTCAGAAA GGTATTGGAG 
CAAGTGCATC CGTCGTTGCA AGCCCGCGAG GATGCTCTGC TCTACGTCGA AAGTTTATGC 
TTGCGTCTGT TAGCGATGCT GTGTGCAAAA CCACCACCGC ATACAGTATT GGACGTCGAA 
GAACGAATTG GGCGTACGTT TCCTACGCCC ATTGACAAAT GGGCCCTGAG CGAGGCACGT 
GAGACAATAG ACAAATCCAA AAAGAAGAAA TCGGTGCTAC CGGTTGACCG AGTCCATACC 
TTGCTGCAAA AGGAGGTCCT GCAATACAAA ATCGATAGTT CGGTTTCATT GTTCCTGGTG 
GCAGTGCTGG AGTACATTTC GGCGGACATC CTGAAACTAG CTGGCAACTA TGTCAAGAAC 
ATTCGCCACA TCGAGATATC CCGGGAGGAT ATCGAGGTCG CCATGTGCGC TGACAAGGTG 
CTCATGGACA TGTTCTACCA GGGTGACAAC TCGAGCTCGA TGGCACCGAG CCCGTTGCCG 
CCGACGCCTC GTACCAGTCT CAGCTACGAA GAGGTGGTCA AAGAGCTCAT TCACGACGAG 
AAGCAGTACC AGCGGGACCT GCACATGATC ATAAGGGTGT TTCGCGAGGA GCTGGTCAAA 
ATCGTCAAGG ACCCCAAGGA ACTTGACCCT ATCTTCTCGA ACATTATGGA TATCTATGAA 
GTGTCTGTAA CGTTACTGGG TTCACTCGAA GATGTGATCG AGATGTCGCA GGAGCAAACT 
CCACCATGCA TCGGAAGCTG TTTCGAAGAA CTAGCGGAAG CGGCAGAGTT TGATGTGTAC 
GCCAAGTATG CGAAAGATAT CACGTCAGTT CAGGCAAAGG AAGCCTTAGC AAATTTATTG 
TCTAGGCCAG AGGCGAATTC ACTGATGTCG GCTGGTCACG GTTTCAGGGA AGCGGTTAAG 
TTCTATCTAC CAAAACTACT TTTAGGGCCT ATCGGGCATG CACAGTTGTA TTTAGATTAT 
ATTAAAATTT TACTACAGCT TAGTCCCTCG CAGGAGGATA AGGAAAGCTT CGAGCAGGTT 
CAGGGTTTGT TAAAACCCTT GCAGTGCGAG TTGCAAGGCA TATCCTCACT GCTGCCTAAG 
GAATATTTCG CTAGGGTAAA TAGTCGAGCT AGACGTCAGT CGGCAATTGA GAAGACTCGC 
GACTTACAAA ACAGTGTAGA ACATTGGGAC AAGGACGTGG GTCAGTGTTG CAACGAGTTC 
ATTCGGGAGG ATACCCTTGC CAAGCTCAGT TCCGGCAGGC GGCAAACCGA ACGGAAGGTG 
TTCCTATTTG ATGGGTTGTT GGTGTTGTGT AAAACCCGAC GACAGATTGT GCCTGGCACC 
AACTACGATT ACCGATTGAA GGAACGCTTT TTCATGCGAA AGGTGGAGAT CATCGATCGG 
CCTGACACCG AGGAGCTCAA GTATGCTTTC GAGATCTCAC CCCGGGAGCC GACTAGCGTT 
GTACTTATTG CTAAAAATGC GCAACACAAA AACGACTGGA TGGCTGATTT GGTTATGCTG 
AACACGAAAT CCATGTTGGA ACGCATTCTA GACAGTATTC TCCTAGATAT TGAAAAGAAA 
CATCCACTCA AAGTACCTAG TCCGGACATA TATGCATTTG CCGTGCCTGA TTCGCCCTCG 
AATATAGTGC TGGAAGAGCG AGAAGGCACG GGAGTTCCAC TGATCAAGGG CGCCACTCTA 
ACGAAACTGA TCGAACGGCT TACCTATCAC ATTTACGCCG ATCCAATGTT CGTGCGTACC 
TTCCTAACAA CGTACCGATC GTTTTGTTCG CCCACAGAGC TTCTTAGGTT ACTGGTGGAA 
CGGTTTGACA TACCGGATCC GGCACTAGTG TACGACAGTG CCAGCGACAA GGATCTAGGG 
CCAGACACGG ACAAGTTTCA TAAAAATTCA CAACGGGAAG ACTGGAAACG CTATAAGAAG 
GAGTATGTCC AACCGGTACA GTTCCGGGTA CTGAACGTTC TTCGGCACTG GGTAGACCAT 
CATTTTTACG ACTTTGAACG TGACTCCGAG CTTCTGGAAG AGTTGTTAAA GTTCCTTGAA 
ACGGTACGCG GTAAATCGAT GCGAAAGTGG GTTGACTCAG TGCTGAAGAT TGTGCAACGG 
AAGACCGAAA GCGAAGATAG TCATCGGCAG ATAACGTTTG CCTTCGGCCA TAGTCCACCG 
GCGATCGAGC ATCACCTACC GCTGACTATT GGTGAGGAGG AATATAACCT GCTGATGTTG 
CATCCCCTGG AACTTGCCCG TCAGCTAACG TTGTTAGAAT TTGAAATGTA TAAAAATGTA 
AAACCATCGG AACTGGTCGG TTCGGTGTGG ACCGGGAAAG ATAAAGAAAC TACTAGTCCT 
AATTTATTAA GAATTATGCA TCACACTACG AATTTCACCC GCTGGATTGA GAAATCAATT 
ATCGAGGCGG AGAATTTCGA CGAACGTGTG GCAATGGCAT CACGTGCAAT AGAGGTGATG 
ATGGTTCTGC AGGATCTAAA CAACTTCAAC GGTGTGCTGT CGATCGTGTC GGCCTTCCAG 
GGGGCGGCCG TCCACCGGCT CAAGCTAACG CTGGAGGAAA TCCCCAAGAG CTACCAGCGG 
GTGCTGGCAG AGTGTCGGGA GCTGAACAAT TCGCACTTCA AAAAGTATCA GGAAAAGCTG 
CGTTCAATAA ATCCACCTTG TGTGCCATTT TTTGGAATGT ATTTAACCAA TATTTTACAT 
ATAGAGGAAG GGAATCCAGA TTTTTTACCA AAGACGAAAC TAATTAACTT CTCTAAGAGA 
AGACGTGTAG CTGAAATCAC TGGTGAAATA CAGCAATATC AAAATCAACC ATACTGTTTG 
AAAGTTGACC CTAGCATTAG GCATTTTCTA GAAAATTTAG ATCCTTTTAC AGGCATGAGT 
GTAACCGAGA TCCAAAACTA TCTCTACGAG GAAAGTAAAA GGATAGAGCC GAAAAATTGT 
CGCCAACCGT TAAAATTCGC ACGCAAGTGG CCAGATATTT CACTAAAGTC TCCCGGCATC 
AAGCCTTCTT CGAGACGACA CAACAGTTCG GCCAACTCGT CATTGAATCT AACGGGTACG 
TTGCCATTTA CGAACAAAGC GCCGCTTGGG AACAGCAACG ATACCGGTGC GGAACAGCAG 
TCACCAACAG CCAGTTCATC GTCGTCTTCC GTGCTCGACT ATTCGATATT TGCTCCTGTG 
AACATTCACT CTTCGTCGTC ATCACAACAC CATCAATACC ATCACCATCA GCAACAAATG 
TACAACTATC AGCATCATTC GCAGGGATCG ATGCAATATT ATCCGGGCCA CCATCAGTCG 
GGTGGTGGCC AGCAGCAAAA CCATCACCAT TATAATCATC ACGGGCATCA GCACAACTTG 
TCCCAGTCAG TGGTCAATCT CGCATCAACG GCAGACCATC AGATGGCGCC GGAGATTCCA 
CGACGTTCCG ATAGTATTAT CCTTACCCCT TCACTATCGT CCCAAGTACA GTACAACCTA 
AACAATAGTA GCTTATCCGA ATCCAGCAGT AATACCGGTG GTGGCAGTAA ACTCGTTCCA 
AGCCCTCGCT ATCCATCGTC ATCAATGAGT GCGCTTTCGT CTTCGTCCTC CGGCATAAGT 
GTTTCTTCGT TCTATGGAAT CATGAGCGCA TCGAACAGTG ATGCGTCTGG TGTAGGGGGC 
GGAGGAAGTC TCAGCATGAG CAGCTCGTCT GCAACCAACG CCGCTAGTAT TGTTTCGCTA 
AGTGAGGGTT CAGCCGTTCC TCAACCCAAC ACCGGTACAG CCATCTCGCC GTGTACGACG 
ATGTCTTCGC CATCGGCCAG CTCGTCCTCG ACCGTGACTG CCGTGGGAAC AACGGTTACT 
GCATCACCAG CACCAGCATC GTCCGGTGCT GCCGACATTC CACCTGCGAT TAGTCCACGC 
ACTGACAAGC CGGTATACCC GCCGGGACCG CCACCCTTAC GGAACCATGG AGGTGGGGCA 
ACCGAGTTTT GCTACAACAC TTCCGGACTA CCGACATCAC CGCTAGCAAC TAGCTGTAAC 
TTTAGGATTA GTTCATCCAA CATTGGAAAG AATCAAAACT GTGATTTTAC GACGGCGAGC 
AGCGCGGGCA ACCGCTCGTC GTGTGACAAT TCGCCGATTT TCCCCTCGAG CCCGCAGATG 
CATCCCATTC ACGGTGGCAC GGCGTCTACG ATGGATTTCA ACAACTGTGC CATCTCGCCG 
ACGCTGTCCA CCTCGGTTTC GTCGACGTCC TCGGTGTACC AGACGACGTA CAATAACTCA 
TCGACGACGG CCGATTCGAT GATCCTCAAC ACTACGGCGG CGAGCAACGA CTTGGGCCCG 
CCCCACTCAC CGCACGTTAA CGTACCAAAT ACACACAATA TTCCTCCTCC TATGCCACCT 
CCTCTACCTC CTAGGGTTAA ACGAAAAGAG TCCTCCGGGG AGTCGTCACA ATCTTCGCAG 
GTGCGGCAAG CACCGGATGC ACCCAAGCTT CCTCCGAGAG ATATTAGTCC ACCTCCGGTA 
CCACCACGTA TCCACAATCC GTCGCATCAG ATATGCGGGA ATGTCCAGCA ACAGCATCAG 
CTGTTACACA GCGTAGGAGC TTGTGACAGC TGCAACCTCG TTAATGTATT ACCGAAGGAC 
GAATCTCCGT CTTCATCATC CTCGTCGTCG ACGCTGACCC GCGACAACTT GAACCAGCAC 
AACATGAGCG AACAGCGGCT ACTCATGCTA CCGCATACCA GCACGATCAT GATGCGACGA 
AACTCCGCCA TGGATCGGGC ATCGAAGGAG AAAGTGTCCA ACATTACCGC ATCGCCGTCG 
CTATCCGGAC TTTCCAGTAC AGTGGGGGGT GTGGTCGGCG GTGGAAGTAC GGCGAGTAGT 
GGACCAGTCA GCTTATCCTC ATCTAGCGCA CCACCACCGC CATGTGGAGC GAAGAACAAA 
TCAGTTCAAA ACTCACCCGT ATCGCAACAG CAGCAGCAAC AACCCGCAGT TTGCGGTCGA 
CGGCCTAGTA CGAATATATC GCCGAGATTT TCACCCGGAG AAACCACACC TAAATTACCT 
CCGAAGCCAA AGCAAACCAA TCTGTCATCA CATTCAGATC GAACAATGTT CCCCTATCCG 
AGCACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021701328.1
CDS	826..6057
Translation	MFAGGQHVSITDATDYDFEKAENAAKWKGVFITSLRKVLEQVHPSLQAREDALLYVESLCLRLLAMLCAKPPPHTVLDVEERIGRTFPTPIDKWALSEARETIDKSKKKKSVLPVDRVHTLLQKEVLQYKIDSSVSLFLVAVLEYISADILKLAGNYVKNIRHIEISREDIEVAMCADKVLMDMFYQGDNSSSMAPSPLPPTPRTSLSYEEVVKELIHDEKQYQRDLHMIIRVFREELVKIVKDPKELDPIFSNIMDIYEVSVTLLGSLEDVIEMSQEQTPPCIGSCFEELAEAAEFDVYAKYAKDITSVQAKEALANLLSRPEANSLMSAGHGFREAVKFYLPKLLLGPIGHAQLYLDYIKILLQLSPSQEDKESFEQVQGLLKPLQCELQGISSLLPKEYFARVNSRARRQSAIEKTRDLQNSVEHWDKDVGQCCNEFIREDTLAKLSSGRRQTERKVFLFDGLLVLCKTRRQIVPGTNYDYRLKERFFMRKVEIIDRPDTEELKYAFEISPREPTSVVLIAKNAQHKNDWMADLVMLNTKSMLERILDSILLDIEKKHPLKVPSPDIYAFAVPDSPSNIVLEEREGTGVPLIKGATLTKLIERLTYHIYADPMFVRTFLTTYRSFCSPTELLRLLVERFDIPDPALVYDSASDKDLGPDTDKFHKNSQREDWKRYKKEYVQPVQFRVLNVLRHWVDHHFYDFERDSELLEELLKFLETVRGKSMRKWVDSVLKIVQRKTESEDSHRQITFAFGHSPPAIEHHLPLTIGEEEYNLLMLHPLELARQLTLLEFEMYKNVKPSELVGSVWTGKDKETTSPNLLRIMHHTTNFTRWIEKSIIEAENFDERVAMASRAIEVMMVLQDLNNFNGVLSIVSAFQGAAVHRLKLTLEEIPKSYQRVLAECRELNNSHFKKYQEKLRSINPPCVPFFGMYLTNILHIEEGNPDFLPKTKLINFSKRRRVAEITGEIQQYQNQPYCLKVDPSIRHFLENLDPFTGMSVTEIQNYLYEESKRIEPKNCRQPLKFARKWPDISLKSPGIKPSSRRHNSSANSSLNLTGTLPFTNKAPLGNSNDTGAEQQSPTASSSSSSVLDYSIFAPVNIHSSSSSQHHQYHHHQQQMYNYQHHSQGSMQYYPGHHQSGGGQQQNHHHYNHHGHQHNLSQSVVNLASTADHQMAPEIPRRSDSIILTPSLSSQVQYNLNNSSLSESSSNTGGGSKLVPSPRYPSSSMSALSSSSSGISVSSFYGIMSASNSDASGVGGGGSLSMSSSSATNAASIVSLSEGSAVPQPNTGTAISPCTTMSSPSASSSSTVTAVGTTVTASPAPASSGAADIPPAISPRTDKPVYPPGPPPLRNHGGGATEFCYNTSGLPTSPLATSCNFRISSSNIGKNQNCDFTTASSAGNRSSCDNSPIFPSSPQMHPIHGGTASTMDFNNCAISPTLSTSVSSTSSVYQTTYNNSSTTADSMILNTTAASNDLGPPHSPHVNVPNTHNIPPPMPPPLPPRVKRKESSGESSQSSQVRQAPDAPKLPPRDISPPPVPPRIHNPSHQICGNVQQQHQLLHSVGACDSCNLVNVLPKDESPSSSSSSSTLTRDNLNQHNMSEQRLLMLPHTSTIMMRRNSAMDRASKEKVSNITASPSLSGLSSTVGGVVGGGSTASSGPVSLSSSSAPPPPCGAKNKSVQNSPVSQQQQQQPAVCGRRPSTNISPRFSPGETTPKLPPKPKQTNLSSHSDRTMFPYPSTN

Target ORF information:

RefSeq Version	XM_021845636.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti protein son of sevenless (LOC5568816), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021845636.1

ORF Insert Sequence:

ATGTTCGCTG GCGGCCAGCA TGTATCGATC ACGGATGCGA CAGATTACGA CTTTGAGAAA 
GCGGAAAATG CAGCCAAATG GAAAGGTGTT TTCATAACAT CGCTCAGAAA GGTATTGGAG 
CAAGTGCATC CGTCGTTGCA AGCCCGCGAG GATGCTCTGC TCTACGTCGA AAGTTTATGC 
TTGCGTCTGT TAGCGATGCT GTGTGCAAAA CCACCACCGC ATACAGTATT GGACGTCGAA 
GAACGAATTG GGCGTACGTT TCCTACGCCC ATTGACAAAT GGGCCCTGAG CGAGGCACGT 
GAGACAATAG ACAAATCCAA AAAGAAGAAA TCGGTGCTAC CGGTTGACCG AGTCCATACC 
TTGCTGCAAA AGGAGGTCCT GCAATACAAA ATCGATAGTT CGGTTTCATT GTTCCTGGTG 
GCAGTGCTGG AGTACATTTC GGCGGACATC CTGAAACTAG CTGGCAACTA TGTCAAGAAC 
ATTCGCCACA TCGAGATATC CCGGGAGGAT ATCGAGGTCG CCATGTGCGC TGACAAGGTG 
CTCATGGACA TGTTCTACCA GGGTGACAAC TCGAGCTCGA TGGCACCGAG CCCGTTGCCG 
CCGACGCCTC GTACCAGTCT CAGCTACGAA GAGGTGGTCA AAGAGCTCAT TCACGACGAG 
AAGCAGTACC AGCGGGACCT GCACATGATC ATAAGGGTGT TTCGCGAGGA GCTGGTCAAA 
ATCGTCAAGG ACCCCAAGGA ACTTGACCCT ATCTTCTCGA ACATTATGGA TATCTATGAA 
GTGTCTGTAA CGTTACTGGG TTCACTCGAA GATGTGATCG AGATGTCGCA GGAGCAAACT 
CCACCATGCA TCGGAAGCTG TTTCGAAGAA CTAGCGGAAG CGGCAGAGTT TGATGTGTAC 
GCCAAGTATG CGAAAGATAT CACGTCAGTT CAGGCAAAGG AAGCCTTAGC AAATTTATTG 
TCTAGGCCAG AGGCGAATTC ACTGATGTCG GCTGGTCACG GTTTCAGGGA AGCGGTTAAG 
TTCTATCTAC CAAAACTACT TTTAGGGCCT ATCGGGCATG CACAGTTGTA TTTAGATTAT 
ATTAAAATTT TACTACAGCT TAGTCCCTCG CAGGAGGATA AGGAAAGCTT CGAGCAGGTT 
CAGGGTTTGT TAAAACCCTT GCAGTGCGAG TTGCAAGGCA TATCCTCACT GCTGCCTAAG 
GAATATTTCG CTAGGGTAAA TAGTCGAGCT AGACGTCAGT CGGCAATTGA GAAGACTCGC 
GACTTACAAA ACAGTGTAGA ACATTGGGAC AAGGACGTGG GTCAGTGTTG CAACGAGTTC 
ATTCGGGAGG ATACCCTTGC CAAGCTCAGT TCCGGCAGGC GGCAAACCGA ACGGAAGGTG 
TTCCTATTTG ATGGGTTGTT GGTGTTGTGT AAAACCCGAC GACAGATTGT GCCTGGCACC 
AACTACGATT ACCGATTGAA GGAACGCTTT TTCATGCGAA AGGTGGAGAT CATCGATCGG 
CCTGACACCG AGGAGCTCAA GTATGCTTTC GAGATCTCAC CCCGGGAGCC GACTAGCGTT 
GTACTTATTG CTAAAAATGC GCAACACAAA AACGACTGGA TGGCTGATTT GGTTATGCTG 
AACACGAAAT CCATGTTGGA ACGCATTCTA GACAGTATTC TCCTAGATAT TGAAAAGAAA 
CATCCACTCA AAGTACCTAG TCCGGACATA TATGCATTTG CCGTGCCTGA TTCGCCCTCG 
AATATAGTGC TGGAAGAGCG AGAAGGCACG GGAGTTCCAC TGATCAAGGG CGCCACTCTA 
ACGAAACTGA TCGAACGGCT TACCTATCAC ATTTACGCCG ATCCAATGTT CGTGCGTACC 
TTCCTAACAA CGTACCGATC GTTTTGTTCG CCCACAGAGC TTCTTAGGTT ACTGGTGGAA 
CGGTTTGACA TACCGGATCC GGCACTAGTG TACGACAGTG CCAGCGACAA GGATCTAGGG 
CCAGACACGG ACAAGTTTCA TAAAAATTCA CAACGGGAAG ACTGGAAACG CTATAAGAAG 
GAGTATGTCC AACCGGTACA GTTCCGGGTA CTGAACGTTC TTCGGCACTG GGTAGACCAT 
CATTTTTACG ACTTTGAACG TGACTCCGAG CTTCTGGAAG AGTTGTTAAA GTTCCTTGAA 
ACGGTACGCG GTAAATCGAT GCGAAAGTGG GTTGACTCAG TGCTGAAGAT TGTGCAACGG 
AAGACCGAAA GCGAAGATAG TCATCGGCAG ATAACGTTTG CCTTCGGCCA TAGTCCACCG 
GCGATCGAGC ATCACCTACC GCTGACTATT GGTGAGGAGG AATATAACCT GCTGATGTTG 
CATCCCCTGG AACTTGCCCG TCAGCTAACG TTGTTAGAAT TTGAAATGTA TAAAAATGTA 
AAACCATCGG AACTGGTCGG TTCGGTGTGG ACCGGGAAAG ATAAAGAAAC TACTAGTCCT 
AATTTATTAA GAATTATGCA TCACACTACG AATTTCACCC GCTGGATTGA GAAATCAATT 
ATCGAGGCGG AGAATTTCGA CGAACGTGTG GCAATGGCAT CACGTGCAAT AGAGGTGATG 
ATGGTTCTGC AGGATCTAAA CAACTTCAAC GGTGTGCTGT CGATCGTGTC GGCCTTCCAG 
GGGGCGGCCG TCCACCGGCT CAAGCTAACG CTGGAGGAAA TCCCCAAGAG CTACCAGCGG 
GTGCTGGCAG AGTGTCGGGA GCTGAACAAT TCGCACTTCA AAAAGTATCA GGAAAAGCTG 
CGTTCAATAA ATCCACCTTG TGTGCCATTT TTTGGAATGT ATTTAACCAA TATTTTACAT 
ATAGAGGAAG GGAATCCAGA TTTTTTACCA AAGACGAAAC TAATTAACTT CTCTAAGAGA 
AGACGTGTAG CTGAAATCAC TGGTGAAATA CAGCAATATC AAAATCAACC ATACTGTTTG 
AAAGTTGACC CTAGCATTAG GCATTTTCTA GAAAATTTAG ATCCTTTTAC AGGCATGAGT 
GTAACCGAGA TCCAAAACTA TCTCTACGAG GAAAGTAAAA GGATAGAGCC GAAAAATTGT 
CGCCAACCGT TAAAATTCGC ACGCAAGTGG CCAGATATTT CACTAAAGTC TCCCGGCATC 
AAGCCTTCTT CGAGACGACA CAACAGTTCG GCCAACTCGT CATTGAATCT AACGGGTACG 
TTGCCATTTA CGAACAAAGC GCCGCTTGGG AACAGCAACG ATACCGGTGC GGAACAGCAG 
TCACCAACAG CCAGTTCATC GTCGTCTTCC GTGCTCGACT ATTCGATATT TGCTCCTGTG 
AACATTCACT CTTCGTCGTC ATCACAACAC CATCAATACC ATCACCATCA GCAACAAATG 
TACAACTATC AGCATCATTC GCAGGGATCG ATGCAATATT ATCCGGGCCA CCATCAGTCG 
GGTGGTGGCC AGCAGCAAAA CCATCACCAT TATAATCATC ACGGGCATCA GCACAACTTG 
TCCCAGTCAG TGGTCAATCT CGCATCAACG GCAGACCATC AGATGGCGCC GGAGATTCCA 
CGACGTTCCG ATAGTATTAT CCTTACCCCT TCACTATCGT CCCAAGTACA GTACAACCTA 
AACAATAGTA GCTTATCCGA ATCCAGCAGT AATACCGGTG GTGGCAGTAA ACTCGTTCCA 
AGCCCTCGCT ATCCATCGTC ATCAATGAGT GCGCTTTCGT CTTCGTCCTC CGGCATAAGT 
GTTTCTTCGT TCTATGGAAT CATGAGCGCA TCGAACAGTG ATGCGTCTGG TGTAGGGGGC 
GGAGGAAGTC TCAGCATGAG CAGCTCGTCT GCAACCAACG CCGCTAGTAT TGTTTCGCTA 
AGTGAGGGTT CAGCCGTTCC TCAACCCAAC ACCGGTACAG CCATCTCGCC GTGTACGACG 
ATGTCTTCGC CATCGGCCAG CTCGTCCTCG ACCGTGACTG CCGTGGGAAC AACGGTTACT 
GCATCACCAG CACCAGCATC GTCCGGTGCT GCCGACATTC CACCTGCGAT TAGTCCACGC 
ACTGACAAGC CGGTATACCC GCCGGGACCG CCACCCTTAC GGAACCATGG AGGTGGGGCA 
ACCGAGTTTT GCTACAACAC TTCCGGACTA CCGACATCAC CGCTAGCAAC TAGCTGTAAC 
TTTAGGATTA GTTCATCCAA CATTGGAAAG AATCAAAACT GTGATTTTAC GACGGCGAGC 
AGCGCGGGCA ACCGCTCGTC GTGTGACAAT TCGCCGATTT TCCCCTCGAG CCCGCAGATG 
CATCCCATTC ACGGTGGCAC GGCGTCTACG ATGGATTTCA ACAACTGTGC CATCTCGCCG 
ACGCTGTCCA CCTCGGTTTC GTCGACGTCC TCGGTGTACC AGACGACGTA CAATAACTCA 
TCGACGACGG CCGATTCGAT GATCCTCAAC ACTACGGCGG CGAGCAACGA CTTGGGCCCG 
CCCCACTCAC CGCACGTTAA CGTACCAAAT ACACACAATA TTCCTCCTCC TATGCCACCT 
CCTCTACCTC CTAGGGTTAA ACGAAAAGAG TCCTCCGGGG AGTCGTCACA ATCTTCGCAG 
GTGCGGCAAG CACCGGATGC ACCCAAGCTT CCTCCGAGAG ATATTAGTCC ACCTCCGGTA 
CCACCACGTA TCCACAATCC GTCGCATCAG ATATGCGGGA ATGTCCAGCA ACAGCATCAG 
CTGTTACACA GCGTAGGAGC TTGTGACAGC TGCAACCTCG TTAATGTATT ACCGAAGGAC 
GAATCTCCGT CTTCATCATC CTCGTCGTCG ACGCTGACCC GCGACAACTT GAACCAGCAC 
AACATGAGCG AACAGCGGCT ACTCATGCTA CCGCATACCA GCACGATCAT GATGCGACGA 
AACTCCGCCA TGGATCGGGC ATCGAAGGAG AAAGTGTCCA ACATTACCGC ATCGCCGTCG 
CTATCCGGAC TTTCCAGTAC AGTGGGGGGT GTGGTCGGCG GTGGAAGTAC GGCGAGTAGT 
GGACCAGTCA GCTTATCCTC ATCTAGCGCA CCACCACCGC CATGTGGAGC GAAGAACAAA 
TCAGTTCAAA ACTCACCCGT ATCGCAACAG CAGCAGCAAC AACCCGCAGT TTGCGGTCGA 
CGGCCTAGTA CGAATATATC GCCGAGATTT TCACCCGGAG AAACCACACC TAAATTACCT 
CCGAAGCCAA AGCAAACCAA TCTGTCATCA CATTCAGATC GAACAATGTT CCCCTATCCG 
AGCACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5568816 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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