AAEL_AAEL006849 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL006849
Entrez Gene ID	5568434
Full Name	voltage-dependent T-type calcium channel subunit alpha-1G
Synonyms	AAEL006842,AAEL006849
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021850463.1	XP_021706155.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
XM_021850467.1	XP_021706159.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
XM_021850464.1	XP_021706156.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
XM_021850465.1	XP_021706157.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
XM_021850466.1	XP_021706158.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
XM_001652216.2	XP_001652266.1	AAEL006849-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL006849 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL006849 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe126300	XM_021850463.1 Latest version!	Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe126301	XM_021850464.1 Latest version!	Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe126302	XM_021850465.1 Latest version!	Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe126303	XM_021850466.1 Latest version!	Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAe126304	XM_021850467.1 Latest version!	Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL006849 ( XM_021850463.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021706155.1 Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAe126300
Clone ID Related Accession (Same CDS sequence)	XM_021850463.1
Accession Version	XM_021850463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACG CCTATTCGGA ATCACAATCC GATTTAGAAA ATGAGCAAAA CTTTGGAGCC 
AAACAGAAAC CACGCCCATC CGTCAACCGT CAATGCCAGG ACAGACGCAG CTCGTTGGTC 
AAACGTAGTG TCTCGGCAAA GAGCAATAGA CGGCCGAGCA TCAACAGCGT CTACAACGAG 
GGCCAGTTCA GCGAATCGTG CAGCATGCGA AAGAAACCTA ACAAATCCGT CGGGTCTGTT 
TCATCCACGA GCGGCACCAC GATGGACTCG TCCGGAAGCA GCTGCAGCGA TGGCGACACC 
TCGTCGTCCT ACGGGGAACC GAATCTTCCA TATCCGGGGT TTGCAGAGTA TTCGCTCAAG 
TATCTCAGCC AGGACACGAA ACCGAGAATA TGGTGCTTGC AGTTGATAAC CAATCCATGG 
TTCGAGCGAG TCTCAATGTT GGTGATTTTG CTGAATTGTG TTACATTGGG AATGTATCAA 
CCATGTGTTG ATGACGCCTG TGTGACGAAC CGCTGTAAAA TATTACAAAT ATTCGACGAT 
ATCATATTCG CTTTCTTTTC CCTCGAAATG ACAATCAAGA TAGTGGCGAT GGGTGCCTGG 
GGGAAGGGAA CCTATCTGGC GGATTCGTGG AACCGGCTGG ACTTCTTCAT TGTGCTGGCC 
GGAGCTTTGG AGTACTGCCT TCAGGTGGAA AACCTTAACC TAACCGCAAT CAGAACCATC 
CGTGTACTTA GACCACTGAG GGCAATCAAT CGGATACCGA GTATGCGAAT TCTTGTCATG 
CTATTGCTAG ACACGCTGCC CATGCTCGGT AATGTATTGC TGTTGTGCTT TTTCGTGTTC 
TTCATCTTCG GGATAGTGGG TGTACAGCTT TGGGAGGGCA TCTTGCGGCA GCGTTGCGTC 
ATAAAGCTTC CGGACAACGT GTCGGCTCCA GACACATTTA CACAACGTAA CCTTTACTAT 
AGAATTGTGA CCCAAAAGCA AAGGAAATCT GACATATCAT TTTACTACGA GTTTTCAAAG 
GAGCAGGACT ACATCTGCTC GAAGCCGGAC GACTCGGGAA TGCATCTGTG TAACAACCTT 
CCACCCTACC GGATAGGGCC ATTGGTTTGC AATGCTTCTG CCATGCCATT TTCGAACAAT 
GTACCCACAG CTCGGTCCTG CGTCAACTGG AATCAGTATT ACACCAACTG TACTCAGCTT 
GGGAACAATC CCTTCCAAGG GACGATATCT TTCGACAACA TCGGATTGGC CTGGGTCGCT 
ATATTTTTGG TCATATCACT CGAGGGATGG ACTGACATTA TGTATTATGT TCAAGACGCA 
CACAGTTTTT GGGATTGGAT ATATTTTGTG CTGCTGATTG TGATCGGATC ATTCTTTATG 
ATCAACCTTT GCCTAGTCGT AATTGCCACA CAGTTTTCGG AAACCAAGAA ACGGGAAATG 
GAGCGTATGC GCCAGGAGAG GGCTCGCTTT ACGTCGACCT CGACTTTGGC TTCCAGTACG 
AACAACTCCG AACCAACGAC TTGCTACGCA GAAATCGTCA AGTATATCGC ACATCTGTAC 
CGAAGGCTCA AGCGCCGCAT CATTAAGAAG TTACGACAGT ATAAATATCA ATACCAACAC 
CGGAAGGAAG GGTTGCTACC GCAAACTCCG GAAACCCTAA CATTGTCACC GAACAAGATC 
AAAGTCCACC ATCCAAAGTG TCCGAAAATG CACGCCCTAG CTGTGAGCAC TGTCCACAGG 
AATAATCAGG GTTCCAACAC GCCCTGTGCC ATCATCCCTA GGAACGCCCC GAGCGCTGTT 
GGCACCGAGA AGCAAATGTC CTCACCGGAC ATTTCGGAAA TGGTTTCCGT GGAGAACATC 
AAGAACAATG CTCTGAACAA TTGCACGAGC TATTCGAACT TGGATGACAA ACAGAAAATA 
GTCCTGCTGA AGATTCCAAA TAGTGAGAAC AATCAGTCCT CTTCAAACTC CCTCAGCCCG 
ACGTCCTCAG GTAGACGCAG ATCATCTGTT ATGTTTAATG AATATGTTAT ACATCATACG 
CCTCCAACTA TTCCAGCACC GACAACAGAT AAAAATGTGT ATTGCTTGGA AAAAATGACC 
CAAACCAGCG ATAGTGGAAT TTGGCAGGTC AGCCTACCAC AATCCATCCA AACCATGGCA 
ACGGTTTTCA ACGACTACTC AGACCTTTGC ATGACAGATG CTATGACCTG TCAAGAGCTA 
TTGGCCTTCT CGGTGGCCTT CTCCGCGGCA CTTCCAACGG GACACACAAC ATTGGAATCG 
TTCTACTCGA CGCTCACCAG AAATCGCACG AATCAAAGCA ATAATAAAAA TGCGTCAATT 
ACCAACATTC AAGGATACAG CGGCGATAAT GTCCAGCAGA AATCCCCGCC CAATGTGGCT 
AAAAATCTAT CGCAAAATGT ATCCAACATC AACAATATTA ACAACGAAGA GTTCGCCTGT 
TGCTACGATC TGTACCAAAA CGTGGCATTG GAAGAACCAC CGAAGAAACA CTCAAAGCCC 
TACAGATGCA TAACGGGAAC ATACAGATTT TTCAAAAGAG TACTACACTG TATCAGAGTG 
TACATCAAAA AGTTAGTGGA GCACAAGTAC TTTCAGCAAG GTATATTGCT GGCCATTTTG 
ATCAATACCC TTTCGATGGG CATCGAGTAC CACAATCAAC CGGAAGAGTT GACGGCAATT 
GTCGAGACGA GTAATATTGT GTTCTCTGGC ATCTTTGCTG TTGAGATGGT TCTTAAGGTG 
ATTGCGGAAG GACCGTTCGG ATATGTCGCT AATGGGTTTA ACGTGTTCGA TGGAGTAATT 
GTCATTCTTA GCGTTGTTGA ACTTGGTCAA GCATACCTGG GTGAAGGTCA AGGCAGTTCT 
GGCTTAAGTG TCCTAAGAAC GTTTCGCCTG CTACGTATAC TAAAGTTGGT ACGATTTATG 
CCAAACCTTC GGCGTCAACT GTTCGTGATG TTGAGAACTA TGGACAATGT GGCTATCTTC 
TTCTCCCTGC TGATACTGTT TATTTTCATA TTCAGCATAC TTGGCATGTA CTTGTTTGGA 
GGTAAGTTCT GTAAGTTTGT CGATGAAACT GGACAAGAGA GGGAATGCAC TTGTCCCGAA 
ATAGTCTCCA AGCATCCTCT GTGTGAATGT GATCGCAAAC ATTTCAACAA CATTCTCTGG 
GCCACGGTGA CAGTGTTTCA AATCCTTACG CAAGAAGATT GGAACGTCGT CCTGTTCAAC 
GGGATGGAGA AGACCAGCCA TTGGGCCGCC TTGTACTTTG TAACGCTCAT GACCTTCGGG 
AATTATGTGT TGTTCAATTT GTTGGTTGCT ATTTTGGTTG AAGGGTTTAG CTCAGAGCGA 
AACGAGCGAA GGGAACGAGA GCAGCGGGAA CTCGTGAAGG CCAAACTCAA TGCCGAGTCC 
TTGGCTGCTC AAGAGCAGGG GAGCGAGATC TACGATGATC CCAAAAGCTT TTCAGAGTCA 
ACCACTAGTG ATAGCTATAA TGAGTCCAAG GGGAAATGGT TCAGCGCTGA GGAGCTTCGG 
AAGATAAGGG ACATAAAGTG CAACATACAG AAACAAAGGC TTTTGCAACC CGGGTATGAT 
GACATAAATC TGTCGATTCC AAAGCTAAAC TCGGAGAAAG ATGCTCTCAA TTCTATAGAT 
CTATATTCCA ACCGAAAGAA AAAATGCGAA CCCCTTAAAC TGTACAACAT CCAAGTGACG 
GATCCTCCGA TCATAACGAC AACTGCCGCA ACTCCTCAAG ATTCGCCGAA CGCAACCCTC 
GAAGGAGGTG CCAGTTTCAA AGATTGGGAA AACCTGGACT TTGAACAACT GGAGAAAAGT 
TCCAGCTCAT CGCTACTTCG TCCCCCACCA ATACTGGGAT CTCTGAAAGC TTTGGACGAT 
CGAACGTACT TCGAAGGCAT GCCGGTGCTA AGTGAAATGC GCAGAAAGTC GGATAAGGGT 
ACCGGTTCTC AAGGTGACAG TAAATCAGCG GACCGGATGC AGCAGTCGAC GCAAGACGGA 
TCACGCAAGT CGGAAACTAC GCGGAAGCCT AGTCTGAAGA GTAGGAAGGA TGCCCCACGA 
GAAGGCGAAG TACTCAACAA CGGCAAATGC GTTTCTCGTA GTGGAGGAAT CACGCCACTA 
GATGCCCGCA AAATCGATCC CCTGTCGCAG AGGAGTAACA GGATACAGAA TGATACCCAA 
AGAGACAGAC GAAATTCGTC CCGAAGAAGT TCCGTAAGGC GATCGTCGTC CATCAAGATC 
GACAGTGGAC CCGGAAGCAT CGCCAGCAAT ATAAGCGCTC CATCGTACTC CAAGTACATC 
TACAACGACA AGAATAAGTA TTACATAGAT AGGAAGAGTT CCCTTCGGTT GAGCGACATA 
AAATCTCCGA ACAACGCCCG GCGAATGTCG TCCATAGAGC ACACGTATCA CAAACATTCG 
AGGGTAAGCG TGCACAATCT GGAATTGAGG AAAATCCAAG AAGAACTAAC GAAAAACAAG 
CTGAACAACG CTTCGGTGAC ATCGGTCCAG GATGTACTGC CCGATGCACC TGATACCCGC 
ATCAAGAACA AATCCAAGGG TCGCCTGAAG CAGTTCTTCC GCTACGTCAC CCCGAAGAAT 
TTTGTCGAGG AACGTGATTT GTACACTTTG TATATTTTCC CGGAGGATAA CAGATTCCGC 
CAGATTTGCA GTTGGTTCGT GAATCAGAAA TGGTTCGACA ATGTGATATT GCTGTTCATA 
GCGCTGAATT GTATAACGCT TGCCATGGAG AGACCCAACA TCCCGCCCAC TTGCACCGAG 
AGATATTTCC TGTCCACTGC GAACTATGTT TTTACGGTTG TATTTGCAGT GGAGATGTTC 
ATCAAGGTTG TCGCTACTGG AATGTTCTAC GGTCGAGATG CGTATTTCAC TTCCGGTTGG 
AACATTATGG ATGGTTCGCT TGTCATAATT TCCATCGTCG ATCTTTTGAT GTCCTTGATC 
AGCGAGTCAA GTCCGAGAAT TTTCGGCATA TTGAGGGTCT TTCGATTGTT AAGGTCGCTT 
AGACCTCTAA GGGTAATCAA CAGAGCACCA GGATTGAAGT TGGTGGTTCA AACACTGCTT 
TCATCATTGA GACCCATCGG AAACATAGTG TTGATATGCT GCACATTTTT CATCATCTTT 
GGAATTCTTG GAGTGCAGCT GTTCAAGGGA ACATTCTACT ACTGCGAAGG GGAGAACATC 
AAAGGTGTCA AGAACAAGAC CGAGTGCTTG TCTATCGAAG GAAATGTTTG GGTAAACCGG 
AAGTACAACT TTGACGATCT GGGTAAGGCG TTGATGTCTC TGTTTGTGCT GTCCTCTCGA 
GATGGCTGGG TGAACATTAT GTACACTGGA CTTGATGCGG TCGGTGTTGA CCAACAGCCA 
GTCGTAAACT ACAACGAGTG GCGCTTGCTG TACTTTATTG CGTTTATCCT GCTGGTGGGA 
TTTTTCGTGT TGAACATGTT TGTGGGAGTT GTGGTCGAAA ACTTTCATCG CTGCCGCGAA 
GAGCAGGAAA AGGAAGAGAA AATACGTCGC GCTGCTAAGC GGGCGTTGCA GATGGAGAAG 
AAACGGCGAC GAATGCACGA ACCGCCGTAC TACACCAACT ACTCGCCACT GCGGATGTTC 
GTGCACAACG TGGTAACATC GAAGTACTTC GACCTGGCAA TAGCCGCCGT CATAGGCTTG 
AACGTGGTGA CGATGGCCAT GGAGTACTAC ATGATGCCTC GAGCGTTGGA ATACGCACTA 
AAGATATTCA ACTATTTCTT CACCGCGGTT TTCATACTGG AAGCCATCAT GAAACTGGTG 
GCGCTGGGCT TGAAGATCTA CATGAAAGAC AAATGGAACC AACTGGACGT GGCTATTGTG 
ATTCTGTCCA TTGTGGGCAT CGTGCTGGAG GAGCTCGAGA CCAATATCAT CCCCATAAAT 
CCTACGATCA TACGAGTCAT GCGAGTGCTT CGAATAGCTC GAGTTCTGAA ACTGCTCAAA 
ATGGCGAAAG GTATACGAGC ATTATTGGAC ACGGTGATGC AGGCGTTGCC ACAGGTCGGA 
AACTTGGGAC TATTGTTCTT CCTGTTGTTT TTCATTTTTG CGGCACTGGG AGTGGAGTTG 
TTCGGCAGAC TGGAATGTTC CGAAGAGGTG CCATGCCAGG GATTGGGCGA GCATGCCCAC 
TTCGCAAACT TCGGTATGGC CTTTTTGACG TTGTTCAGAG TTGCGACGGG AGATAACTGG 
AATGGGATCA TGAAGGACAC ACTTCGAGAC GACTGTGACG ATGCTGCCGA TTGTGTTAAG 
AATTGTTGTG TTAGTACTAT AATAGCTCCC ATATTTTTCG TTATATTTGT ACTGATGGCT 
CAGTTTGTAC TGGTCAACGT GGTGGTTGCA GTTCTTATGA AGCATCTTGA AGAAAGCCAC 
AAACAGATGG AAGATGAGTT AGATATTGAA ACAGAGCTGG AAAGGGAATT CGAACGTGAA 
CAAGAATTTG AGGAGGAACA AGCATTGTGT ATGCAACTTA ATGACGACCA AAAGCAATTG 
CAAAAACGGC CTCTCACTAA AGTGTCTTCT TTGCCATCTA ACTTTACTTA TAGCACTCCA 
GTTGCGGAGA AAAAATTAAA CATTCATCGA CGGCAAACTA TTCAATACAT TAATCAGAAT 
CTCAATGTAC AACATTCTTA TACAAACAGC AATTCGTATG ACGAAACTGC TGAAAACGAT 
ATTCCGAATG AAGACGACGA GTCTTTCAAT TCTGTCAATG AAAGCTCTAG TACAAAGAAT 
ACCAACAAGA AAATCCATAT TAACAAATCT TTCTTTAGTA AAAATAACGA AGATAAGCGA 
GCTTCATTGG ATGAATTTAG CAAACCGCAC AAAATTGTGG AGGAATCGGG AAAACCCGAT 
AAACCGAGCT CAACGAAGGG TTCAAACAAA TGGAAAGACA ATCAGTCTGA CCAACCCTCG 
AAAGAAGCGG AAAGCAATAC CAAACTGTCG ACAATCATTT CGTCCAACAA ATCGTTGACG 
AAAAGCAACA AACCCGACTA TCGTCAATTA AGTCTTGATC AAGACGCCAA GCACGACAAC 
GTTACGCTTG CGCCCAACAC TAGTTCTTCT AATTTCTCAA ACGCCTCCAC TGCCCTCAAC 
AACCTCGGTG GCAATAAATC CTCCTTTCTA TCCGTACCCA AACTGCAGCC AAAAAGTCGT 
TCAGGCAGCA CAAAGCAACT GTTCAAACAA GTGGCACTGG ACGAAGATAA TGAGACCAAC 
GAGAGTTCGC TCCTGCTGCC TTCGGTATCG AATGCAGCCG GCTCTGGTGG CGATCACTCG 
TACGATCTTC TGAAGGTGCA GGACGAAAGT TACGATGCTG TGCGAAAATC CGAATCCTGC 
GAGATTCTGA GGATCATTTC CGAGCGACGG AAGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021706155.1
CDS	167..7585
Translation	MLNAYSESQSDLENEQNFGAKQKPRPSVNRQCQDRRSSLVKRSVSAKSNRRPSINSVYNEGQFSESCSMRKKPNKSVGSVSSTSGTTMDSSGSSCSDGDTSSSYGEPNLPYPGFAEYSLKYLSQDTKPRIWCLQLITNPWFERVSMLVILLNCVTLGMYQPCVDDACVTNRCKILQIFDDIIFAFFSLEMTIKIVAMGAWGKGTYLADSWNRLDFFIVLAGALEYCLQVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWEGILRQRCVIKLPDNVSAPDTFTQRNLYYRIVTQKQRKSDISFYYEFSKEQDYICSKPDDSGMHLCNNLPPYRIGPLVCNASAMPFSNNVPTARSCVNWNQYYTNCTQLGNNPFQGTISFDNIGLAWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERARFTSTSTLASSTNNSEPTTCYAEIVKYIAHLYRRLKRRIIKKLRQYKYQYQHRKEGLLPQTPETLTLSPNKIKVHHPKCPKMHALAVSTVHRNNQGSNTPCAIIPRNAPSAVGTEKQMSSPDISEMVSVENIKNNALNNCTSYSNLDDKQKIVLLKIPNSENNQSSSNSLSPTSSGRRRSSVMFNEYVIHHTPPTIPAPTTDKNVYCLEKMTQTSDSGIWQVSLPQSIQTMATVFNDYSDLCMTDAMTCQELLAFSVAFSAALPTGHTTLESFYSTLTRNRTNQSNNKNASITNIQGYSGDNVQQKSPPNVAKNLSQNVSNINNINNEEFACCYDLYQNVALEEPPKKHSKPYRCITGTYRFFKRVLHCIRVYIKKLVEHKYFQQGILLAILINTLSMGIEYHNQPEELTAIVETSNIVFSGIFAVEMVLKVIAEGPFGYVANGFNVFDGVIVILSVVELGQAYLGEGQGSSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAIFFSLLILFIFIFSILGMYLFGGKFCKFVDETGQERECTCPEIVSKHPLCECDRKHFNNILWATVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVTLMTFGNYVLFNLLVAILVEGFSSERNERREREQRELVKAKLNAESLAAQEQGSEIYDDPKSFSESTTSDSYNESKGKWFSAEELRKIRDIKCNIQKQRLLQPGYDDINLSIPKLNSEKDALNSIDLYSNRKKKCEPLKLYNIQVTDPPIITTTAATPQDSPNATLEGGASFKDWENLDFEQLEKSSSSSLLRPPPILGSLKALDDRTYFEGMPVLSEMRRKSDKGTGSQGDSKSADRMQQSTQDGSRKSETTRKPSLKSRKDAPREGEVLNNGKCVSRSGGITPLDARKIDPLSQRSNRIQNDTQRDRRNSSRRSSVRRSSSIKIDSGPGSIASNISAPSYSKYIYNDKNKYYIDRKSSLRLSDIKSPNNARRMSSIEHTYHKHSRVSVHNLELRKIQEELTKNKLNNASVTSVQDVLPDAPDTRIKNKSKGRLKQFFRYVTPKNFVEERDLYTLYIFPEDNRFRQICSWFVNQKWFDNVILLFIALNCITLAMERPNIPPTCTERYFLSTANYVFTVVFAVEMFIKVVATGMFYGRDAYFTSGWNIMDGSLVIISIVDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVKNKTECLSIEGNVWVNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPVVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPLRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYMMPRALEYALKIFNYFFTAVFILEAIMKLVALGLKIYMKDKWNQLDVAIVILSIVGIVLEELETNIIPINPTIIRVMRVLRIARVLKLLKMAKGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSEEVPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDDCDDAADCVKNCCVSTIIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHKQMEDELDIETELEREFEREQEFEEEQALCMQLNDDQKQLQKRPLTKVSSLPSNFTYSTPVAEKKLNIHRRQTIQYINQNLNVQHSYTNSNSYDETAENDIPNEDDESFNSVNESSSTKNTNKKIHINKSFFSKNNEDKRASLDEFSKPHKIVEESGKPDKPSSTKGSNKWKDNQSDQPSKEAESNTKLSTIISSNKSLTKSNKPDYRQLSLDQDAKHDNVTLAPNTSSSNFSNASTALNNLGGNKSSFLSVPKLQPKSRSGSTKQLFKQVALDEDNETNESSLLLPSVSNAAGSGGDHSYDLLKVQDESYDAVRKSESCEILRIISERRKI

Target ORF information:

RefSeq Version	XM_021850463.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021850463.1

ORF Insert Sequence:

ATGTTGAACG CCTATTCGGA ATCACAATCC GATTTAGAAA ATGAGCAAAA CTTTGGAGCC 
AAACAGAAAC CACGCCCATC CGTCAACCGT CAATGCCAGG ACAGACGCAG CTCGTTGGTC 
AAACGTAGTG TCTCGGCAAA GAGCAATAGA CGGCCGAGCA TCAACAGCGT CTACAACGAG 
GGCCAGTTCA GCGAATCGTG CAGCATGCGA AAGAAACCTA ACAAATCCGT CGGGTCTGTT 
TCATCCACGA GCGGCACCAC GATGGACTCG TCCGGAAGCA GCTGCAGCGA TGGCGACACC 
TCGTCGTCCT ACGGGGAACC GAATCTTCCA TATCCGGGGT TTGCAGAGTA TTCGCTCAAG 
TATCTCAGCC AGGACACGAA ACCGAGAATA TGGTGCTTGC AGTTGATAAC CAATCCATGG 
TTCGAGCGAG TCTCAATGTT GGTGATTTTG CTGAATTGTG TTACATTGGG AATGTATCAA 
CCATGTGTTG ATGACGCCTG TGTGACGAAC CGCTGTAAAA TATTACAAAT ATTCGACGAT 
ATCATATTCG CTTTCTTTTC CCTCGAAATG ACAATCAAGA TAGTGGCGAT GGGTGCCTGG 
GGGAAGGGAA CCTATCTGGC GGATTCGTGG AACCGGCTGG ACTTCTTCAT TGTGCTGGCC 
GGAGCTTTGG AGTACTGCCT TCAGGTGGAA AACCTTAACC TAACCGCAAT CAGAACCATC 
CGTGTACTTA GACCACTGAG GGCAATCAAT CGGATACCGA GTATGCGAAT TCTTGTCATG 
CTATTGCTAG ACACGCTGCC CATGCTCGGT AATGTATTGC TGTTGTGCTT TTTCGTGTTC 
TTCATCTTCG GGATAGTGGG TGTACAGCTT TGGGAGGGCA TCTTGCGGCA GCGTTGCGTC 
ATAAAGCTTC CGGACAACGT GTCGGCTCCA GACACATTTA CACAACGTAA CCTTTACTAT 
AGAATTGTGA CCCAAAAGCA AAGGAAATCT GACATATCAT TTTACTACGA GTTTTCAAAG 
GAGCAGGACT ACATCTGCTC GAAGCCGGAC GACTCGGGAA TGCATCTGTG TAACAACCTT 
CCACCCTACC GGATAGGGCC ATTGGTTTGC AATGCTTCTG CCATGCCATT TTCGAACAAT 
GTACCCACAG CTCGGTCCTG CGTCAACTGG AATCAGTATT ACACCAACTG TACTCAGCTT 
GGGAACAATC CCTTCCAAGG GACGATATCT TTCGACAACA TCGGATTGGC CTGGGTCGCT 
ATATTTTTGG TCATATCACT CGAGGGATGG ACTGACATTA TGTATTATGT TCAAGACGCA 
CACAGTTTTT GGGATTGGAT ATATTTTGTG CTGCTGATTG TGATCGGATC ATTCTTTATG 
ATCAACCTTT GCCTAGTCGT AATTGCCACA CAGTTTTCGG AAACCAAGAA ACGGGAAATG 
GAGCGTATGC GCCAGGAGAG GGCTCGCTTT ACGTCGACCT CGACTTTGGC TTCCAGTACG 
AACAACTCCG AACCAACGAC TTGCTACGCA GAAATCGTCA AGTATATCGC ACATCTGTAC 
CGAAGGCTCA AGCGCCGCAT CATTAAGAAG TTACGACAGT ATAAATATCA ATACCAACAC 
CGGAAGGAAG GGTTGCTACC GCAAACTCCG GAAACCCTAA CATTGTCACC GAACAAGATC 
AAAGTCCACC ATCCAAAGTG TCCGAAAATG CACGCCCTAG CTGTGAGCAC TGTCCACAGG 
AATAATCAGG GTTCCAACAC GCCCTGTGCC ATCATCCCTA GGAACGCCCC GAGCGCTGTT 
GGCACCGAGA AGCAAATGTC CTCACCGGAC ATTTCGGAAA TGGTTTCCGT GGAGAACATC 
AAGAACAATG CTCTGAACAA TTGCACGAGC TATTCGAACT TGGATGACAA ACAGAAAATA 
GTCCTGCTGA AGATTCCAAA TAGTGAGAAC AATCAGTCCT CTTCAAACTC CCTCAGCCCG 
ACGTCCTCAG GTAGACGCAG ATCATCTGTT ATGTTTAATG AATATGTTAT ACATCATACG 
CCTCCAACTA TTCCAGCACC GACAACAGAT AAAAATGTGT ATTGCTTGGA AAAAATGACC 
CAAACCAGCG ATAGTGGAAT TTGGCAGGTC AGCCTACCAC AATCCATCCA AACCATGGCA 
ACGGTTTTCA ACGACTACTC AGACCTTTGC ATGACAGATG CTATGACCTG TCAAGAGCTA 
TTGGCCTTCT CGGTGGCCTT CTCCGCGGCA CTTCCAACGG GACACACAAC ATTGGAATCG 
TTCTACTCGA CGCTCACCAG AAATCGCACG AATCAAAGCA ATAATAAAAA TGCGTCAATT 
ACCAACATTC AAGGATACAG CGGCGATAAT GTCCAGCAGA AATCCCCGCC CAATGTGGCT 
AAAAATCTAT CGCAAAATGT ATCCAACATC AACAATATTA ACAACGAAGA GTTCGCCTGT 
TGCTACGATC TGTACCAAAA CGTGGCATTG GAAGAACCAC CGAAGAAACA CTCAAAGCCC 
TACAGATGCA TAACGGGAAC ATACAGATTT TTCAAAAGAG TACTACACTG TATCAGAGTG 
TACATCAAAA AGTTAGTGGA GCACAAGTAC TTTCAGCAAG GTATATTGCT GGCCATTTTG 
ATCAATACCC TTTCGATGGG CATCGAGTAC CACAATCAAC CGGAAGAGTT GACGGCAATT 
GTCGAGACGA GTAATATTGT GTTCTCTGGC ATCTTTGCTG TTGAGATGGT TCTTAAGGTG 
ATTGCGGAAG GACCGTTCGG ATATGTCGCT AATGGGTTTA ACGTGTTCGA TGGAGTAATT 
GTCATTCTTA GCGTTGTTGA ACTTGGTCAA GCATACCTGG GTGAAGGTCA AGGCAGTTCT 
GGCTTAAGTG TCCTAAGAAC GTTTCGCCTG CTACGTATAC TAAAGTTGGT ACGATTTATG 
CCAAACCTTC GGCGTCAACT GTTCGTGATG TTGAGAACTA TGGACAATGT GGCTATCTTC 
TTCTCCCTGC TGATACTGTT TATTTTCATA TTCAGCATAC TTGGCATGTA CTTGTTTGGA 
GGTAAGTTCT GTAAGTTTGT CGATGAAACT GGACAAGAGA GGGAATGCAC TTGTCCCGAA 
ATAGTCTCCA AGCATCCTCT GTGTGAATGT GATCGCAAAC ATTTCAACAA CATTCTCTGG 
GCCACGGTGA CAGTGTTTCA AATCCTTACG CAAGAAGATT GGAACGTCGT CCTGTTCAAC 
GGGATGGAGA AGACCAGCCA TTGGGCCGCC TTGTACTTTG TAACGCTCAT GACCTTCGGG 
AATTATGTGT TGTTCAATTT GTTGGTTGCT ATTTTGGTTG AAGGGTTTAG CTCAGAGCGA 
AACGAGCGAA GGGAACGAGA GCAGCGGGAA CTCGTGAAGG CCAAACTCAA TGCCGAGTCC 
TTGGCTGCTC AAGAGCAGGG GAGCGAGATC TACGATGATC CCAAAAGCTT TTCAGAGTCA 
ACCACTAGTG ATAGCTATAA TGAGTCCAAG GGGAAATGGT TCAGCGCTGA GGAGCTTCGG 
AAGATAAGGG ACATAAAGTG CAACATACAG AAACAAAGGC TTTTGCAACC CGGGTATGAT 
GACATAAATC TGTCGATTCC AAAGCTAAAC TCGGAGAAAG ATGCTCTCAA TTCTATAGAT 
CTATATTCCA ACCGAAAGAA AAAATGCGAA CCCCTTAAAC TGTACAACAT CCAAGTGACG 
GATCCTCCGA TCATAACGAC AACTGCCGCA ACTCCTCAAG ATTCGCCGAA CGCAACCCTC 
GAAGGAGGTG CCAGTTTCAA AGATTGGGAA AACCTGGACT TTGAACAACT GGAGAAAAGT 
TCCAGCTCAT CGCTACTTCG TCCCCCACCA ATACTGGGAT CTCTGAAAGC TTTGGACGAT 
CGAACGTACT TCGAAGGCAT GCCGGTGCTA AGTGAAATGC GCAGAAAGTC GGATAAGGGT 
ACCGGTTCTC AAGGTGACAG TAAATCAGCG GACCGGATGC AGCAGTCGAC GCAAGACGGA 
TCACGCAAGT CGGAAACTAC GCGGAAGCCT AGTCTGAAGA GTAGGAAGGA TGCCCCACGA 
GAAGGCGAAG TACTCAACAA CGGCAAATGC GTTTCTCGTA GTGGAGGAAT CACGCCACTA 
GATGCCCGCA AAATCGATCC CCTGTCGCAG AGGAGTAACA GGATACAGAA TGATACCCAA 
AGAGACAGAC GAAATTCGTC CCGAAGAAGT TCCGTAAGGC GATCGTCGTC CATCAAGATC 
GACAGTGGAC CCGGAAGCAT CGCCAGCAAT ATAAGCGCTC CATCGTACTC CAAGTACATC 
TACAACGACA AGAATAAGTA TTACATAGAT AGGAAGAGTT CCCTTCGGTT GAGCGACATA 
AAATCTCCGA ACAACGCCCG GCGAATGTCG TCCATAGAGC ACACGTATCA CAAACATTCG 
AGGGTAAGCG TGCACAATCT GGAATTGAGG AAAATCCAAG AAGAACTAAC GAAAAACAAG 
CTGAACAACG CTTCGGTGAC ATCGGTCCAG GATGTACTGC CCGATGCACC TGATACCCGC 
ATCAAGAACA AATCCAAGGG TCGCCTGAAG CAGTTCTTCC GCTACGTCAC CCCGAAGAAT 
TTTGTCGAGG AACGTGATTT GTACACTTTG TATATTTTCC CGGAGGATAA CAGATTCCGC 
CAGATTTGCA GTTGGTTCGT GAATCAGAAA TGGTTCGACA ATGTGATATT GCTGTTCATA 
GCGCTGAATT GTATAACGCT TGCCATGGAG AGACCCAACA TCCCGCCCAC TTGCACCGAG 
AGATATTTCC TGTCCACTGC GAACTATGTT TTTACGGTTG TATTTGCAGT GGAGATGTTC 
ATCAAGGTTG TCGCTACTGG AATGTTCTAC GGTCGAGATG CGTATTTCAC TTCCGGTTGG 
AACATTATGG ATGGTTCGCT TGTCATAATT TCCATCGTCG ATCTTTTGAT GTCCTTGATC 
AGCGAGTCAA GTCCGAGAAT TTTCGGCATA TTGAGGGTCT TTCGATTGTT AAGGTCGCTT 
AGACCTCTAA GGGTAATCAA CAGAGCACCA GGATTGAAGT TGGTGGTTCA AACACTGCTT 
TCATCATTGA GACCCATCGG AAACATAGTG TTGATATGCT GCACATTTTT CATCATCTTT 
GGAATTCTTG GAGTGCAGCT GTTCAAGGGA ACATTCTACT ACTGCGAAGG GGAGAACATC 
AAAGGTGTCA AGAACAAGAC CGAGTGCTTG TCTATCGAAG GAAATGTTTG GGTAAACCGG 
AAGTACAACT TTGACGATCT GGGTAAGGCG TTGATGTCTC TGTTTGTGCT GTCCTCTCGA 
GATGGCTGGG TGAACATTAT GTACACTGGA CTTGATGCGG TCGGTGTTGA CCAACAGCCA 
GTCGTAAACT ACAACGAGTG GCGCTTGCTG TACTTTATTG CGTTTATCCT GCTGGTGGGA 
TTTTTCGTGT TGAACATGTT TGTGGGAGTT GTGGTCGAAA ACTTTCATCG CTGCCGCGAA 
GAGCAGGAAA AGGAAGAGAA AATACGTCGC GCTGCTAAGC GGGCGTTGCA GATGGAGAAG 
AAACGGCGAC GAATGCACGA ACCGCCGTAC TACACCAACT ACTCGCCACT GCGGATGTTC 
GTGCACAACG TGGTAACATC GAAGTACTTC GACCTGGCAA TAGCCGCCGT CATAGGCTTG 
AACGTGGTGA CGATGGCCAT GGAGTACTAC ATGATGCCTC GAGCGTTGGA ATACGCACTA 
AAGATATTCA ACTATTTCTT CACCGCGGTT TTCATACTGG AAGCCATCAT GAAACTGGTG 
GCGCTGGGCT TGAAGATCTA CATGAAAGAC AAATGGAACC AACTGGACGT GGCTATTGTG 
ATTCTGTCCA TTGTGGGCAT CGTGCTGGAG GAGCTCGAGA CCAATATCAT CCCCATAAAT 
CCTACGATCA TACGAGTCAT GCGAGTGCTT CGAATAGCTC GAGTTCTGAA ACTGCTCAAA 
ATGGCGAAAG GTATACGAGC ATTATTGGAC ACGGTGATGC AGGCGTTGCC ACAGGTCGGA 
AACTTGGGAC TATTGTTCTT CCTGTTGTTT TTCATTTTTG CGGCACTGGG AGTGGAGTTG 
TTCGGCAGAC TGGAATGTTC CGAAGAGGTG CCATGCCAGG GATTGGGCGA GCATGCCCAC 
TTCGCAAACT TCGGTATGGC CTTTTTGACG TTGTTCAGAG TTGCGACGGG AGATAACTGG 
AATGGGATCA TGAAGGACAC ACTTCGAGAC GACTGTGACG ATGCTGCCGA TTGTGTTAAG 
AATTGTTGTG TTAGTACTAT AATAGCTCCC ATATTTTTCG TTATATTTGT ACTGATGGCT 
CAGTTTGTAC TGGTCAACGT GGTGGTTGCA GTTCTTATGA AGCATCTTGA AGAAAGCCAC 
AAACAGATGG AAGATGAGTT AGATATTGAA ACAGAGCTGG AAAGGGAATT CGAACGTGAA 
CAAGAATTTG AGGAGGAACA AGCATTGTGT ATGCAACTTA ATGACGACCA AAAGCAATTG 
CAAAAACGGC CTCTCACTAA AGTGTCTTCT TTGCCATCTA ACTTTACTTA TAGCACTCCA 
GTTGCGGAGA AAAAATTAAA CATTCATCGA CGGCAAACTA TTCAATACAT TAATCAGAAT 
CTCAATGTAC AACATTCTTA TACAAACAGC AATTCGTATG ACGAAACTGC TGAAAACGAT 
ATTCCGAATG AAGACGACGA GTCTTTCAAT TCTGTCAATG AAAGCTCTAG TACAAAGAAT 
ACCAACAAGA AAATCCATAT TAACAAATCT TTCTTTAGTA AAAATAACGA AGATAAGCGA 
GCTTCATTGG ATGAATTTAG CAAACCGCAC AAAATTGTGG AGGAATCGGG AAAACCCGAT 
AAACCGAGCT CAACGAAGGG TTCAAACAAA TGGAAAGACA ATCAGTCTGA CCAACCCTCG 
AAAGAAGCGG AAAGCAATAC CAAACTGTCG ACAATCATTT CGTCCAACAA ATCGTTGACG 
AAAAGCAACA AACCCGACTA TCGTCAATTA AGTCTTGATC AAGACGCCAA GCACGACAAC 
GTTACGCTTG CGCCCAACAC TAGTTCTTCT AATTTCTCAA ACGCCTCCAC TGCCCTCAAC 
AACCTCGGTG GCAATAAATC CTCCTTTCTA TCCGTACCCA AACTGCAGCC AAAAAGTCGT 
TCAGGCAGCA CAAAGCAACT GTTCAAACAA GTGGCACTGG ACGAAGATAA TGAGACCAAC 
GAGAGTTCGC TCCTGCTGCC TTCGGTATCG AATGCAGCCG GCTCTGGTGG CGATCACTCG 
TACGATCTTC TGAAGGTGCA GGACGAAAGT TACGATGCTG TGCGAAAATC CGAATCCTGC 
GAGATTCTGA GGATCATTTC CGAGCGACGG AAGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL006849 ( XM_021850464.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021706156.1 Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X2, mRNA.

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CloneID	OAe126301
Clone ID Related Accession (Same CDS sequence)	XM_021850464.1
Accession Version	XM_021850464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACG CCTATTCGGA ATCACAATCC GATTTAGAAA ATGAGCAAAA CTTTGGAGCC 
AAACAGAAAC CACGCCCATC CGTCAACCGT CAATGCCAGG ACAGACGCAG CTCGTTGGTC 
AAACGTAGTG TCTCGGCAAA GAGCAATAGA CGGCCGAGCA TCAACAGCGT CTACAACGAG 
GGCCAGTTCA GCGAATCGTG CAGCATGCGA AAGAAACCTA ACAAATCCGT CGGGTCTGTT 
TCATCCACGA GCGGCACCAC GATGGACTCG TCCGGAAGCA GCTGCAGCGA TGGCGACACC 
TCGTCGTCCT ACGGGGAACC GAATCTTCCA TATCCGGGGT TTGCAGAGTA TTCGCTCAAG 
TATCTCAGCC AGGACACGAA ACCGAGAATA TGGTGCTTGC AGTTGATAAC CAATCCATGG 
TTCGAGCGAG TCTCAATGTT GGTGATTTTG CTGAATTGTG TTACATTGGG AATGTATCAA 
CCATGTGTTG ATGACGCCTG TGTGACGAAC CGCTGTAAAA TATTACAAAT ATTCGACGAT 
ATCATATTCG CTTTCTTTTC CCTCGAAATG ACAATCAAGA TAGTGGCGAT GGGTGCCTGG 
GGGAAGGGAA CCTATCTGGC GGATTCGTGG AACCGGCTGG ACTTCTTCAT TGTGCTGGCC 
GGAGCTTTGG AGTACTGCCT TCAGGTGGAA AACCTTAACC TAACCGCAAT CAGAACCATC 
CGTGTACTTA GACCACTGAG GGCAATCAAT CGGATACCGA GTATGCGAAT TCTTGTCATG 
CTATTGCTAG ACACGCTGCC CATGCTCGGT AATGTATTGC TGTTGTGCTT TTTCGTGTTC 
TTCATCTTCG GGATAGTGGG TGTACAGCTT TGGGAGGGCA TCTTGCGGCA GCGTTGCGTC 
ATAAAGCTTC CGGACAACGT GTCGGCTCCA GACACATTTA CACAACGTAA CCTTTACTAT 
AGAATTGTGA CCCAAAAGCA AAGGAAATCT GACATATCAT TTTACTACGA GTTTTCAAAG 
GAGCAGGACT ACATCTGCTC GAAGCCGGAC GACTCGGGAA TGCATCTGTG TAACAACCTT 
CCACCCTACC GGATAGGGCC ATTGGTTTGC AATGCTTCTG CCATGCCATT TTCGAACAAT 
GTACCCACAG CTCGGTCCTG CGTCAACTGG AATCAGTATT ACACCAACTG TACTCAGCTT 
GGGAACAATC CCTTCCAAGG GACGATATCT TTCGACAACA TCGGATTGGC CTGGGTCGCT 
ATATTTTTGG TCATATCACT CGAGGGATGG ACTGACATTA TGTATTATGT TCAAGACGCA 
CACAGTTTTT GGGATTGGAT ATATTTTGTG CTGCTGATTG TGATCGGATC ATTCTTTATG 
ATCAACCTTT GCCTAGTCGT AATTGCCACA CAGTTTTCGG AAACCAAGAA ACGGGAAATG 
GAGCGTATGC GCCAGGAGAG GGCTCGCTTT ACGTCGACCT CGACTTTGGC TTCCAGTACG 
AACAACTCCG AACCAACGAC TTGCTACGCA GAAATCGTCA AGTATATCGC ACATCTGTAC 
CGAAGGCTCA AGCGCCGCAT CATTAAGAAA TATCAATACC AACACCGGAA GGAAGGGTTG 
CTACCGCAAA CTCCGGAAAC CCTAACATTG TCACCGAACA AGATCAAAGT CCACCATCCA 
AAGTGTCCGA AAATGCACGC CCTAGCTGTG AGCACTGTCC ACAGGAATAA TCAGGGTTCC 
AACACGCCCT GTGCCATCAT CCCTAGGAAC GCCCCGAGCG CTGTTGGCAC CGAGAAGCAA 
ATGTCCTCAC CGGACATTTC GGAAATGGTT TCCGTGGAGA ACATCAAGAA CAATGCTCTG 
AACAATTGCA CGAGCTATTC GAACTTGGAT GACAAACAGA AAATAGTCCT GCTGAAGATT 
CCAAATAGTG AGAACAATCA GTCCTCTTCA AACTCCCTCA GCCCGACGTC CTCAGGTAGA 
CGCAGATCAT CTGTTATGTT TAATGAATAT GTTATACATC ATACGCCTCC AACTATTCCA 
GCACCGACAA CAGATAAAAA TGTGTATTGC TTGGAAAAAA TGACCCAAAC CAGCGATAGT 
GGAATTTGGC AGGTCAGCCT ACCACAATCC ATCCAAACCA TGGCAACGGT TTTCAACGAC 
TACTCAGACC TTTGCATGAC AGATGCTATG ACCTGTCAAG AGCTATTGGC CTTCTCGGTG 
GCCTTCTCCG CGGCACTTCC AACGGGACAC ACAACATTGG AATCGTTCTA CTCGACGCTC 
ACCAGAAATC GCACGAATCA AAGCAATAAT AAAAATGCGT CAATTACCAA CATTCAAGGA 
TACAGCGGCG ATAATGTCCA GCAGAAATCC CCGCCCAATG TGGCTAAAAA TCTATCGCAA 
AATGTATCCA ACATCAACAA TATTAACAAC GAAGAGTTCG CCTGTTGCTA CGATCTGTAC 
CAAAACGTGG CATTGGAAGA ACCACCGAAG AAACACTCAA AGCCCTACAG ATGCATAACG 
GGAACATACA GATTTTTCAA AAGAGTACTA CACTGTATCA GAGTGTACAT CAAAAAGTTA 
GTGGAGCACA AGTACTTTCA GCAAGGTATA TTGCTGGCCA TTTTGATCAA TACCCTTTCG 
ATGGGCATCG AGTACCACAA TCAACCGGAA GAGTTGACGG CAATTGTCGA GACGAGTAAT 
ATTGTGTTCT CTGGCATCTT TGCTGTTGAG ATGGTTCTTA AGGTGATTGC GGAAGGACCG 
TTCGGATATG TCGCTAATGG GTTTAACGTG TTCGATGGAG TAATTGTCAT TCTTAGCGTT 
GTTGAACTTG GTCAAGCATA CCTGGGTGAA GGTCAAGGCA GTTCTGGCTT AAGTGTCCTA 
AGAACGTTTC GCCTGCTACG TATACTAAAG TTGGTACGAT TTATGCCAAA CCTTCGGCGT 
CAACTGTTCG TGATGTTGAG AACTATGGAC AATGTGGCTA TCTTCTTCTC CCTGCTGATA 
CTGTTTATTT TCATATTCAG CATACTTGGC ATGTACTTGT TTGGAGGTAA GTTCTGTAAG 
TTTGTCGATG AAACTGGACA AGAGAGGGAA TGCACTTGTC CCGAAATAGT CTCCAAGCAT 
CCTCTGTGTG AATGTGATCG CAAACATTTC AACAACATTC TCTGGGCCAC GGTGACAGTG 
TTTCAAATCC TTACGCAAGA AGATTGGAAC GTCGTCCTGT TCAACGGGAT GGAGAAGACC 
AGCCATTGGG CCGCCTTGTA CTTTGTAACG CTCATGACCT TCGGGAATTA TGTGTTGTTC 
AATTTGTTGG TTGCTATTTT GGTTGAAGGG TTTAGCTCAG AGCGAAACGA GCGAAGGGAA 
CGAGAGCAGC GGGAACTCGT GAAGGCCAAA CTCAATGCCG AGTCCTTGGC TGCTCAAGAG 
CAGGGGAGCG AGATCTACGA TGATCCCAAA AGCTTTTCAG AGTCAACCAC TAGTGATAGC 
TATAATGAGT CCAAGGGGAA ATGGTTCAGC GCTGAGGAGC TTCGGAAGAT AAGGGACATA 
AAGTGCAACA TACAGAAACA AAGGCTTTTG CAACCCGGGT ATGATGACAT AAATCTGTCG 
ATTCCAAAGC TAAACTCGGA GAAAGATGCT CTCAATTCTA TAGATCTATA TTCCAACCGA 
AAGAAAAAAT GCGAACCCCT TAAACTGTAC AACATCCAAG TGACGGATCC TCCGATCATA 
ACGACAACTG CCGCAACTCC TCAAGATTCG CCGAACGCAA CCCTCGAAGG AGGTGCCAGT 
TTCAAAGATT GGGAAAACCT GGACTTTGAA CAACTGGAGA AAAGTTCCAG CTCATCGCTA 
CTTCGTCCCC CACCAATACT GGGATCTCTG AAAGCTTTGG ACGATCGAAC GTACTTCGAA 
GGCATGCCGG TGCTAAGTGA AATGCGCAGA AAGTCGGATA AGGGTACCGG TTCTCAAGGT 
GACAGTAAAT CAGCGGACCG GATGCAGCAG TCGACGCAAG ACGGATCACG CAAGTCGGAA 
ACTACGCGGA AGCCTAGTCT GAAGAGTAGG AAGGATGCCC CACGAGAAGG CGAAGTACTC 
AACAACGGCA AATGCGTTTC TCGTAGTGGA GGAATCACGC CACTAGATGC CCGCAAAATC 
GATCCCCTGT CGCAGAGGAG TAACAGGATA CAGAATGATA CCCAAAGAGA CAGACGAAAT 
TCGTCCCGAA GAAGTTCCGT AAGGCGATCG TCGTCCATCA AGATCGACAG TGGACCCGGA 
AGCATCGCCA GCAATATAAG CGCTCCATCG TACTCCAAGT ACATCTACAA CGACAAGAAT 
AAGTATTACA TAGATAGGAA GAGTTCCCTT CGGTTGAGCG ACATAAAATC TCCGAACAAC 
GCCCGGCGAA TGTCGTCCAT AGAGCACACG TATCACAAAC ATTCGAGGGT AAGCGTGCAC 
AATCTGGAAT TGAGGAAAAT CCAAGAAGAA CTAACGAAAA ACAAGCTGAA CAACGCTTCG 
GTGACATCGG TCCAGGATGT ACTGCCCGAT GCACCTGATA CCCGCATCAA GAACAAATCC 
AAGGGTCGCC TGAAGCAGTT CTTCCGCTAC GTCACCCCGA AGAATTTTGT CGAGGAACGT 
GATTTGTACA CTTTGTATAT TTTCCCGGAG GATAACAGAT TCCGCCAGAT TTGCAGTTGG 
TTCGTGAATC AGAAATGGTT CGACAATGTG ATATTGCTGT TCATAGCGCT GAATTGTATA 
ACGCTTGCCA TGGAGAGACC CAACATCCCG CCCACTTGCA CCGAGAGATA TTTCCTGTCC 
ACTGCGAACT ATGTTTTTAC GGTTGTATTT GCAGTGGAGA TGTTCATCAA GGTTGTCGCT 
ACTGGAATGT TCTACGGTCG AGATGCGTAT TTCACTTCCG GTTGGAACAT TATGGATGGT 
TCGCTTGTCA TAATTTCCAT CGTCGATCTT TTGATGTCCT TGATCAGCGA GTCAAGTCCG 
AGAATTTTCG GCATATTGAG GGTCTTTCGA TTGTTAAGGT CGCTTAGACC TCTAAGGGTA 
ATCAACAGAG CACCAGGATT GAAGTTGGTG GTTCAAACAC TGCTTTCATC ATTGAGACCC 
ATCGGAAACA TAGTGTTGAT ATGCTGCACA TTTTTCATCA TCTTTGGAAT TCTTGGAGTG 
CAGCTGTTCA AGGGAACATT CTACTACTGC GAAGGGGAGA ACATCAAAGG TGTCAAGAAC 
AAGACCGAGT GCTTGTCTAT CGAAGGAAAT GTTTGGGTAA ACCGGAAGTA CAACTTTGAC 
GATCTGGGTA AGGCGTTGAT GTCTCTGTTT GTGCTGTCCT CTCGAGATGG CTGGGTGAAC 
ATTATGTACA CTGGACTTGA TGCGGTCGGT GTTGACCAAC AGCCAGTCGT AAACTACAAC 
GAGTGGCGCT TGCTGTACTT TATTGCGTTT ATCCTGCTGG TGGGATTTTT CGTGTTGAAC 
ATGTTTGTGG GAGTTGTGGT CGAAAACTTT CATCGCTGCC GCGAAGAGCA GGAAAAGGAA 
GAGAAAATAC GTCGCGCTGC TAAGCGGGCG TTGCAGATGG AGAAGAAACG GCGACGAATG 
CACGAACCGC CGTACTACAC CAACTACTCG CCACTGCGGA TGTTCGTGCA CAACGTGGTA 
ACATCGAAGT ACTTCGACCT GGCAATAGCC GCCGTCATAG GCTTGAACGT GGTGACGATG 
GCCATGGAGT ACTACATGAT GCCTCGAGCG TTGGAATACG CACTAAAGAT ATTCAACTAT 
TTCTTCACCG CGGTTTTCAT ACTGGAAGCC ATCATGAAAC TGGTGGCGCT GGGCTTGAAG 
ATCTACATGA AAGACAAATG GAACCAACTG GACGTGGCTA TTGTGATTCT GTCCATTGTG 
GGCATCGTGC TGGAGGAGCT CGAGACCAAT ATCATCCCCA TAAATCCTAC GATCATACGA 
GTCATGCGAG TGCTTCGAAT AGCTCGAGTT CTGAAACTGC TCAAAATGGC GAAAGGTATA 
CGAGCATTAT TGGACACGGT GATGCAGGCG TTGCCACAGG TCGGAAACTT GGGACTATTG 
TTCTTCCTGT TGTTTTTCAT TTTTGCGGCA CTGGGAGTGG AGTTGTTCGG CAGACTGGAA 
TGTTCCGAAG AGGTGCCATG CCAGGGATTG GGCGAGCATG CCCACTTCGC AAACTTCGGT 
ATGGCCTTTT TGACGTTGTT CAGAGTTGCG ACGGGAGATA ACTGGAATGG GATCATGAAG 
GACACACTTC GAGACGACTG TGACGATGCT GCCGATTGTG TTAAGAATTG TTGTGTTAGT 
ACTATAATAG CTCCCATATT TTTCGTTATA TTTGTACTGA TGGCTCAGTT TGTACTGGTC 
AACGTGGTGG TTGCAGTTCT TATGAAGCAT CTTGAAGAAA GCCACAAACA GATGGAAGAT 
GAGTTAGATA TTGAAACAGA GCTGGAAAGG GAATTCGAAC GTGAACAAGA ATTTGAGGAG 
GAACAAGCAT TGTGTATGCA ACTTAATGAC GACCAAAAGC AATTGCAAAA ACGGCCTCTC 
ACTAAAGTGT CTTCTTTGCC ATCTAACTTT ACTTATAGCA CTCCAGTTGC GGAGAAAAAA 
TTAAACATTC ATCGACGGCA AACTATTCAA TACATTAATC AGAATCTCAA TGTACAACAT 
TCTTATACAA ACAGCAATTC GTATGACGAA ACTGCTGAAA ACGATATTCC GAATGAAGAC 
GACGAGTCTT TCAATTCTGT CAATGAAAGC TCTAGTACAA AGAATACCAA CAAGAAAATC 
CATATTAACA AATCTTTCTT TAGTAAAAAT AACGAAGATA AGCGAGCTTC ATTGGATGAA 
TTTAGCAAAC CGCACAAAAT TGTGGAGGAA TCGGGAAAAC CCGATAAACC GAGCTCAACG 
AAGGGTTCAA ACAAATGGAA AGACAATCAG TCTGACCAAC CCTCGAAAGA AGCGGAAAGC 
AATACCAAAC TGTCGACAAT CATTTCGTCC AACAAATCGT TGACGAAAAG CAACAAACCC 
GACTATCGTC AATTAAGTCT TGATCAAGAC GCCAAGCACG ACAACGTTAC GCTTGCGCCC 
AACACTAGTT CTTCTAATTT CTCAAACGCC TCCACTGCCC TCAACAACCT CGGTGGCAAT 
AAATCCTCCT TTCTATCCGT ACCCAAACTG CAGCCAAAAA GTCGTTCAGG CAGCACAAAG 
CAACTGTTCA AACAAGTGGC ACTGGACGAA GATAATGAGA CCAACGAGAG TTCGCTCCTG 
CTGCCTTCGG TATCGAATGC AGCCGGCTCT GGTGGCGATC ACTCGTACGA TCTTCTGAAG 
GTGCAGGACG AAAGTTACGA TGCTGTGCGA AAATCCGAAT CCTGCGAGAT TCTGAGGATC 
ATTTCCGAGC GACGGAAGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021706156.1
CDS	167..7570
Translation	MLNAYSESQSDLENEQNFGAKQKPRPSVNRQCQDRRSSLVKRSVSAKSNRRPSINSVYNEGQFSESCSMRKKPNKSVGSVSSTSGTTMDSSGSSCSDGDTSSSYGEPNLPYPGFAEYSLKYLSQDTKPRIWCLQLITNPWFERVSMLVILLNCVTLGMYQPCVDDACVTNRCKILQIFDDIIFAFFSLEMTIKIVAMGAWGKGTYLADSWNRLDFFIVLAGALEYCLQVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWEGILRQRCVIKLPDNVSAPDTFTQRNLYYRIVTQKQRKSDISFYYEFSKEQDYICSKPDDSGMHLCNNLPPYRIGPLVCNASAMPFSNNVPTARSCVNWNQYYTNCTQLGNNPFQGTISFDNIGLAWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERARFTSTSTLASSTNNSEPTTCYAEIVKYIAHLYRRLKRRIIKKYQYQHRKEGLLPQTPETLTLSPNKIKVHHPKCPKMHALAVSTVHRNNQGSNTPCAIIPRNAPSAVGTEKQMSSPDISEMVSVENIKNNALNNCTSYSNLDDKQKIVLLKIPNSENNQSSSNSLSPTSSGRRRSSVMFNEYVIHHTPPTIPAPTTDKNVYCLEKMTQTSDSGIWQVSLPQSIQTMATVFNDYSDLCMTDAMTCQELLAFSVAFSAALPTGHTTLESFYSTLTRNRTNQSNNKNASITNIQGYSGDNVQQKSPPNVAKNLSQNVSNINNINNEEFACCYDLYQNVALEEPPKKHSKPYRCITGTYRFFKRVLHCIRVYIKKLVEHKYFQQGILLAILINTLSMGIEYHNQPEELTAIVETSNIVFSGIFAVEMVLKVIAEGPFGYVANGFNVFDGVIVILSVVELGQAYLGEGQGSSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAIFFSLLILFIFIFSILGMYLFGGKFCKFVDETGQERECTCPEIVSKHPLCECDRKHFNNILWATVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVTLMTFGNYVLFNLLVAILVEGFSSERNERREREQRELVKAKLNAESLAAQEQGSEIYDDPKSFSESTTSDSYNESKGKWFSAEELRKIRDIKCNIQKQRLLQPGYDDINLSIPKLNSEKDALNSIDLYSNRKKKCEPLKLYNIQVTDPPIITTTAATPQDSPNATLEGGASFKDWENLDFEQLEKSSSSSLLRPPPILGSLKALDDRTYFEGMPVLSEMRRKSDKGTGSQGDSKSADRMQQSTQDGSRKSETTRKPSLKSRKDAPREGEVLNNGKCVSRSGGITPLDARKIDPLSQRSNRIQNDTQRDRRNSSRRSSVRRSSSIKIDSGPGSIASNISAPSYSKYIYNDKNKYYIDRKSSLRLSDIKSPNNARRMSSIEHTYHKHSRVSVHNLELRKIQEELTKNKLNNASVTSVQDVLPDAPDTRIKNKSKGRLKQFFRYVTPKNFVEERDLYTLYIFPEDNRFRQICSWFVNQKWFDNVILLFIALNCITLAMERPNIPPTCTERYFLSTANYVFTVVFAVEMFIKVVATGMFYGRDAYFTSGWNIMDGSLVIISIVDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVKNKTECLSIEGNVWVNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPVVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPLRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYMMPRALEYALKIFNYFFTAVFILEAIMKLVALGLKIYMKDKWNQLDVAIVILSIVGIVLEELETNIIPINPTIIRVMRVLRIARVLKLLKMAKGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSEEVPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDDCDDAADCVKNCCVSTIIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHKQMEDELDIETELEREFEREQEFEEEQALCMQLNDDQKQLQKRPLTKVSSLPSNFTYSTPVAEKKLNIHRRQTIQYINQNLNVQHSYTNSNSYDETAENDIPNEDDESFNSVNESSSTKNTNKKIHINKSFFSKNNEDKRASLDEFSKPHKIVEESGKPDKPSSTKGSNKWKDNQSDQPSKEAESNTKLSTIISSNKSLTKSNKPDYRQLSLDQDAKHDNVTLAPNTSSSNFSNASTALNNLGGNKSSFLSVPKLQPKSRSGSTKQLFKQVALDEDNETNESSLLLPSVSNAAGSGGDHSYDLLKVQDESYDAVRKSESCEILRIISERRKI

Target ORF information:

RefSeq Version	XM_021850464.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021850464.1

ORF Insert Sequence:

ATGTTGAACG CCTATTCGGA ATCACAATCC GATTTAGAAA ATGAGCAAAA CTTTGGAGCC 
AAACAGAAAC CACGCCCATC CGTCAACCGT CAATGCCAGG ACAGACGCAG CTCGTTGGTC 
AAACGTAGTG TCTCGGCAAA GAGCAATAGA CGGCCGAGCA TCAACAGCGT CTACAACGAG 
GGCCAGTTCA GCGAATCGTG CAGCATGCGA AAGAAACCTA ACAAATCCGT CGGGTCTGTT 
TCATCCACGA GCGGCACCAC GATGGACTCG TCCGGAAGCA GCTGCAGCGA TGGCGACACC 
TCGTCGTCCT ACGGGGAACC GAATCTTCCA TATCCGGGGT TTGCAGAGTA TTCGCTCAAG 
TATCTCAGCC AGGACACGAA ACCGAGAATA TGGTGCTTGC AGTTGATAAC CAATCCATGG 
TTCGAGCGAG TCTCAATGTT GGTGATTTTG CTGAATTGTG TTACATTGGG AATGTATCAA 
CCATGTGTTG ATGACGCCTG TGTGACGAAC CGCTGTAAAA TATTACAAAT ATTCGACGAT 
ATCATATTCG CTTTCTTTTC CCTCGAAATG ACAATCAAGA TAGTGGCGAT GGGTGCCTGG 
GGGAAGGGAA CCTATCTGGC GGATTCGTGG AACCGGCTGG ACTTCTTCAT TGTGCTGGCC 
GGAGCTTTGG AGTACTGCCT TCAGGTGGAA AACCTTAACC TAACCGCAAT CAGAACCATC 
CGTGTACTTA GACCACTGAG GGCAATCAAT CGGATACCGA GTATGCGAAT TCTTGTCATG 
CTATTGCTAG ACACGCTGCC CATGCTCGGT AATGTATTGC TGTTGTGCTT TTTCGTGTTC 
TTCATCTTCG GGATAGTGGG TGTACAGCTT TGGGAGGGCA TCTTGCGGCA GCGTTGCGTC 
ATAAAGCTTC CGGACAACGT GTCGGCTCCA GACACATTTA CACAACGTAA CCTTTACTAT 
AGAATTGTGA CCCAAAAGCA AAGGAAATCT GACATATCAT TTTACTACGA GTTTTCAAAG 
GAGCAGGACT ACATCTGCTC GAAGCCGGAC GACTCGGGAA TGCATCTGTG TAACAACCTT 
CCACCCTACC GGATAGGGCC ATTGGTTTGC AATGCTTCTG CCATGCCATT TTCGAACAAT 
GTACCCACAG CTCGGTCCTG CGTCAACTGG AATCAGTATT ACACCAACTG TACTCAGCTT 
GGGAACAATC CCTTCCAAGG GACGATATCT TTCGACAACA TCGGATTGGC CTGGGTCGCT 
ATATTTTTGG TCATATCACT CGAGGGATGG ACTGACATTA TGTATTATGT TCAAGACGCA 
CACAGTTTTT GGGATTGGAT ATATTTTGTG CTGCTGATTG TGATCGGATC ATTCTTTATG 
ATCAACCTTT GCCTAGTCGT AATTGCCACA CAGTTTTCGG AAACCAAGAA ACGGGAAATG 
GAGCGTATGC GCCAGGAGAG GGCTCGCTTT ACGTCGACCT CGACTTTGGC TTCCAGTACG 
AACAACTCCG AACCAACGAC TTGCTACGCA GAAATCGTCA AGTATATCGC ACATCTGTAC 
CGAAGGCTCA AGCGCCGCAT CATTAAGAAA TATCAATACC AACACCGGAA GGAAGGGTTG 
CTACCGCAAA CTCCGGAAAC CCTAACATTG TCACCGAACA AGATCAAAGT CCACCATCCA 
AAGTGTCCGA AAATGCACGC CCTAGCTGTG AGCACTGTCC ACAGGAATAA TCAGGGTTCC 
AACACGCCCT GTGCCATCAT CCCTAGGAAC GCCCCGAGCG CTGTTGGCAC CGAGAAGCAA 
ATGTCCTCAC CGGACATTTC GGAAATGGTT TCCGTGGAGA ACATCAAGAA CAATGCTCTG 
AACAATTGCA CGAGCTATTC GAACTTGGAT GACAAACAGA AAATAGTCCT GCTGAAGATT 
CCAAATAGTG AGAACAATCA GTCCTCTTCA AACTCCCTCA GCCCGACGTC CTCAGGTAGA 
CGCAGATCAT CTGTTATGTT TAATGAATAT GTTATACATC ATACGCCTCC AACTATTCCA 
GCACCGACAA CAGATAAAAA TGTGTATTGC TTGGAAAAAA TGACCCAAAC CAGCGATAGT 
GGAATTTGGC AGGTCAGCCT ACCACAATCC ATCCAAACCA TGGCAACGGT TTTCAACGAC 
TACTCAGACC TTTGCATGAC AGATGCTATG ACCTGTCAAG AGCTATTGGC CTTCTCGGTG 
GCCTTCTCCG CGGCACTTCC AACGGGACAC ACAACATTGG AATCGTTCTA CTCGACGCTC 
ACCAGAAATC GCACGAATCA AAGCAATAAT AAAAATGCGT CAATTACCAA CATTCAAGGA 
TACAGCGGCG ATAATGTCCA GCAGAAATCC CCGCCCAATG TGGCTAAAAA TCTATCGCAA 
AATGTATCCA ACATCAACAA TATTAACAAC GAAGAGTTCG CCTGTTGCTA CGATCTGTAC 
CAAAACGTGG CATTGGAAGA ACCACCGAAG AAACACTCAA AGCCCTACAG ATGCATAACG 
GGAACATACA GATTTTTCAA AAGAGTACTA CACTGTATCA GAGTGTACAT CAAAAAGTTA 
GTGGAGCACA AGTACTTTCA GCAAGGTATA TTGCTGGCCA TTTTGATCAA TACCCTTTCG 
ATGGGCATCG AGTACCACAA TCAACCGGAA GAGTTGACGG CAATTGTCGA GACGAGTAAT 
ATTGTGTTCT CTGGCATCTT TGCTGTTGAG ATGGTTCTTA AGGTGATTGC GGAAGGACCG 
TTCGGATATG TCGCTAATGG GTTTAACGTG TTCGATGGAG TAATTGTCAT TCTTAGCGTT 
GTTGAACTTG GTCAAGCATA CCTGGGTGAA GGTCAAGGCA GTTCTGGCTT AAGTGTCCTA 
AGAACGTTTC GCCTGCTACG TATACTAAAG TTGGTACGAT TTATGCCAAA CCTTCGGCGT 
CAACTGTTCG TGATGTTGAG AACTATGGAC AATGTGGCTA TCTTCTTCTC CCTGCTGATA 
CTGTTTATTT TCATATTCAG CATACTTGGC ATGTACTTGT TTGGAGGTAA GTTCTGTAAG 
TTTGTCGATG AAACTGGACA AGAGAGGGAA TGCACTTGTC CCGAAATAGT CTCCAAGCAT 
CCTCTGTGTG AATGTGATCG CAAACATTTC AACAACATTC TCTGGGCCAC GGTGACAGTG 
TTTCAAATCC TTACGCAAGA AGATTGGAAC GTCGTCCTGT TCAACGGGAT GGAGAAGACC 
AGCCATTGGG CCGCCTTGTA CTTTGTAACG CTCATGACCT TCGGGAATTA TGTGTTGTTC 
AATTTGTTGG TTGCTATTTT GGTTGAAGGG TTTAGCTCAG AGCGAAACGA GCGAAGGGAA 
CGAGAGCAGC GGGAACTCGT GAAGGCCAAA CTCAATGCCG AGTCCTTGGC TGCTCAAGAG 
CAGGGGAGCG AGATCTACGA TGATCCCAAA AGCTTTTCAG AGTCAACCAC TAGTGATAGC 
TATAATGAGT CCAAGGGGAA ATGGTTCAGC GCTGAGGAGC TTCGGAAGAT AAGGGACATA 
AAGTGCAACA TACAGAAACA AAGGCTTTTG CAACCCGGGT ATGATGACAT AAATCTGTCG 
ATTCCAAAGC TAAACTCGGA GAAAGATGCT CTCAATTCTA TAGATCTATA TTCCAACCGA 
AAGAAAAAAT GCGAACCCCT TAAACTGTAC AACATCCAAG TGACGGATCC TCCGATCATA 
ACGACAACTG CCGCAACTCC TCAAGATTCG CCGAACGCAA CCCTCGAAGG AGGTGCCAGT 
TTCAAAGATT GGGAAAACCT GGACTTTGAA CAACTGGAGA AAAGTTCCAG CTCATCGCTA 
CTTCGTCCCC CACCAATACT GGGATCTCTG AAAGCTTTGG ACGATCGAAC GTACTTCGAA 
GGCATGCCGG TGCTAAGTGA AATGCGCAGA AAGTCGGATA AGGGTACCGG TTCTCAAGGT 
GACAGTAAAT CAGCGGACCG GATGCAGCAG TCGACGCAAG ACGGATCACG CAAGTCGGAA 
ACTACGCGGA AGCCTAGTCT GAAGAGTAGG AAGGATGCCC CACGAGAAGG CGAAGTACTC 
AACAACGGCA AATGCGTTTC TCGTAGTGGA GGAATCACGC CACTAGATGC CCGCAAAATC 
GATCCCCTGT CGCAGAGGAG TAACAGGATA CAGAATGATA CCCAAAGAGA CAGACGAAAT 
TCGTCCCGAA GAAGTTCCGT AAGGCGATCG TCGTCCATCA AGATCGACAG TGGACCCGGA 
AGCATCGCCA GCAATATAAG CGCTCCATCG TACTCCAAGT ACATCTACAA CGACAAGAAT 
AAGTATTACA TAGATAGGAA GAGTTCCCTT CGGTTGAGCG ACATAAAATC TCCGAACAAC 
GCCCGGCGAA TGTCGTCCAT AGAGCACACG TATCACAAAC ATTCGAGGGT AAGCGTGCAC 
AATCTGGAAT TGAGGAAAAT CCAAGAAGAA CTAACGAAAA ACAAGCTGAA CAACGCTTCG 
GTGACATCGG TCCAGGATGT ACTGCCCGAT GCACCTGATA CCCGCATCAA GAACAAATCC 
AAGGGTCGCC TGAAGCAGTT CTTCCGCTAC GTCACCCCGA AGAATTTTGT CGAGGAACGT 
GATTTGTACA CTTTGTATAT TTTCCCGGAG GATAACAGAT TCCGCCAGAT TTGCAGTTGG 
TTCGTGAATC AGAAATGGTT CGACAATGTG ATATTGCTGT TCATAGCGCT GAATTGTATA 
ACGCTTGCCA TGGAGAGACC CAACATCCCG CCCACTTGCA CCGAGAGATA TTTCCTGTCC 
ACTGCGAACT ATGTTTTTAC GGTTGTATTT GCAGTGGAGA TGTTCATCAA GGTTGTCGCT 
ACTGGAATGT TCTACGGTCG AGATGCGTAT TTCACTTCCG GTTGGAACAT TATGGATGGT 
TCGCTTGTCA TAATTTCCAT CGTCGATCTT TTGATGTCCT TGATCAGCGA GTCAAGTCCG 
AGAATTTTCG GCATATTGAG GGTCTTTCGA TTGTTAAGGT CGCTTAGACC TCTAAGGGTA 
ATCAACAGAG CACCAGGATT GAAGTTGGTG GTTCAAACAC TGCTTTCATC ATTGAGACCC 
ATCGGAAACA TAGTGTTGAT ATGCTGCACA TTTTTCATCA TCTTTGGAAT TCTTGGAGTG 
CAGCTGTTCA AGGGAACATT CTACTACTGC GAAGGGGAGA ACATCAAAGG TGTCAAGAAC 
AAGACCGAGT GCTTGTCTAT CGAAGGAAAT GTTTGGGTAA ACCGGAAGTA CAACTTTGAC 
GATCTGGGTA AGGCGTTGAT GTCTCTGTTT GTGCTGTCCT CTCGAGATGG CTGGGTGAAC 
ATTATGTACA CTGGACTTGA TGCGGTCGGT GTTGACCAAC AGCCAGTCGT AAACTACAAC 
GAGTGGCGCT TGCTGTACTT TATTGCGTTT ATCCTGCTGG TGGGATTTTT CGTGTTGAAC 
ATGTTTGTGG GAGTTGTGGT CGAAAACTTT CATCGCTGCC GCGAAGAGCA GGAAAAGGAA 
GAGAAAATAC GTCGCGCTGC TAAGCGGGCG TTGCAGATGG AGAAGAAACG GCGACGAATG 
CACGAACCGC CGTACTACAC CAACTACTCG CCACTGCGGA TGTTCGTGCA CAACGTGGTA 
ACATCGAAGT ACTTCGACCT GGCAATAGCC GCCGTCATAG GCTTGAACGT GGTGACGATG 
GCCATGGAGT ACTACATGAT GCCTCGAGCG TTGGAATACG CACTAAAGAT ATTCAACTAT 
TTCTTCACCG CGGTTTTCAT ACTGGAAGCC ATCATGAAAC TGGTGGCGCT GGGCTTGAAG 
ATCTACATGA AAGACAAATG GAACCAACTG GACGTGGCTA TTGTGATTCT GTCCATTGTG 
GGCATCGTGC TGGAGGAGCT CGAGACCAAT ATCATCCCCA TAAATCCTAC GATCATACGA 
GTCATGCGAG TGCTTCGAAT AGCTCGAGTT CTGAAACTGC TCAAAATGGC GAAAGGTATA 
CGAGCATTAT TGGACACGGT GATGCAGGCG TTGCCACAGG TCGGAAACTT GGGACTATTG 
TTCTTCCTGT TGTTTTTCAT TTTTGCGGCA CTGGGAGTGG AGTTGTTCGG CAGACTGGAA 
TGTTCCGAAG AGGTGCCATG CCAGGGATTG GGCGAGCATG CCCACTTCGC AAACTTCGGT 
ATGGCCTTTT TGACGTTGTT CAGAGTTGCG ACGGGAGATA ACTGGAATGG GATCATGAAG 
GACACACTTC GAGACGACTG TGACGATGCT GCCGATTGTG TTAAGAATTG TTGTGTTAGT 
ACTATAATAG CTCCCATATT TTTCGTTATA TTTGTACTGA TGGCTCAGTT TGTACTGGTC 
AACGTGGTGG TTGCAGTTCT TATGAAGCAT CTTGAAGAAA GCCACAAACA GATGGAAGAT 
GAGTTAGATA TTGAAACAGA GCTGGAAAGG GAATTCGAAC GTGAACAAGA ATTTGAGGAG 
GAACAAGCAT TGTGTATGCA ACTTAATGAC GACCAAAAGC AATTGCAAAA ACGGCCTCTC 
ACTAAAGTGT CTTCTTTGCC ATCTAACTTT ACTTATAGCA CTCCAGTTGC GGAGAAAAAA 
TTAAACATTC ATCGACGGCA AACTATTCAA TACATTAATC AGAATCTCAA TGTACAACAT 
TCTTATACAA ACAGCAATTC GTATGACGAA ACTGCTGAAA ACGATATTCC GAATGAAGAC 
GACGAGTCTT TCAATTCTGT CAATGAAAGC TCTAGTACAA AGAATACCAA CAAGAAAATC 
CATATTAACA AATCTTTCTT TAGTAAAAAT AACGAAGATA AGCGAGCTTC ATTGGATGAA 
TTTAGCAAAC CGCACAAAAT TGTGGAGGAA TCGGGAAAAC CCGATAAACC GAGCTCAACG 
AAGGGTTCAA ACAAATGGAA AGACAATCAG TCTGACCAAC CCTCGAAAGA AGCGGAAAGC 
AATACCAAAC TGTCGACAAT CATTTCGTCC AACAAATCGT TGACGAAAAG CAACAAACCC 
GACTATCGTC AATTAAGTCT TGATCAAGAC GCCAAGCACG ACAACGTTAC GCTTGCGCCC 
AACACTAGTT CTTCTAATTT CTCAAACGCC TCCACTGCCC TCAACAACCT CGGTGGCAAT 
AAATCCTCCT TTCTATCCGT ACCCAAACTG CAGCCAAAAA GTCGTTCAGG CAGCACAAAG 
CAACTGTTCA AACAAGTGGC ACTGGACGAA GATAATGAGA CCAACGAGAG TTCGCTCCTG 
CTGCCTTCGG TATCGAATGC AGCCGGCTCT GGTGGCGATC ACTCGTACGA TCTTCTGAAG 
GTGCAGGACG AAAGTTACGA TGCTGTGCGA AAATCCGAAT CCTGCGAGAT TCTGAGGATC 
ATTTCCGAGC GACGGAAGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL006849 ( XM_021850465.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021706157.1 Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X3, mRNA.

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CloneID	OAe126302
Clone ID Related Accession (Same CDS sequence)	XM_021850465.1
Accession Version	XM_021850465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACG CCTATTCGGA ATCACAATCC GATTTAGAAA ATGAGCAAAA CTTTGGAGCC 
AAACAGAAAC CACGCCCATC CGTCAACCGT CAATGCCAGG ACAGACGCAG CTCGTTGGTC 
AAACGTAGTG TCTCGGCAAA GAGCAATAGA CGGCCGAGCA TCAACAGCGT CTACAACGAG 
GGCCAGTTCA GCGAATCGTG CAGCATGCGA AAGAAACCTA ACAAATCCGT CGGGTCTGTT 
TCATCCACGA GCGGCACCAC GATGGACTCG TCCGGAAGCA GCTGCAGCGA TGGCGACACC 
TCGTCGTCCT ACGGGGAACC GAATCTTCCA TATCCGGGGT TTGCAGAGTA TTCGCTCAAG 
TATCTCAGCC AGGACACGAA ACCGAGAATA TGGTGCTTGC AGTTGATAAC CAATCCATGG 
TTCGAGCGAG TCTCAATGTT GGTGATTTTG CTGAATTGTG TTACATTGGG AATGTATCAA 
CCATGTGTTG ATGACGCCTG TGTGACGAAC CGCTGTAAAA TATTACAAAT ATTCGACGAT 
ATCATATTCG CTTTCTTTTC CCTCGAAATG ACAATCAAGA TAGTGGCGAT GGGTGCCTGG 
GGGAAGGGAA CCTATCTGGC GGATTCGTGG AACCGGCTGG ACTTCTTCAT TGTGCTGGCC 
GGAGCTTTGG AGTACTGCCT TCAGGTGGAA AACCTTAACC TAACCGCAAT CAGAACCATC 
CGTGTACTTA GACCACTGAG GGCAATCAAT CGGATACCGA GTATGCGAAT TCTTGTCATG 
CTATTGCTAG ACACGCTGCC CATGCTCGGT AATGTATTGC TGTTGTGCTT TTTCGTGTTC 
TTCATCTTCG GGATAGTGGG TGTACAGCTT TGGGAGGGCA TCTTGCGGCA GCGTTGCGTC 
ATAAAGCTTC CGGACAACGT GTCGGCTCCA GACATATCAT TTTACTACGA GTTTTCAAAG 
GAGCAGGACT ACATCTGCTC GAAGCCGGAC GACTCGGGAA TGCATCTGTG TAACAACCTT 
CCACCCTACC GGATAGGGCC ATTGGTTTGC AATGCTTCTG CCATGCCATT TTCGAACAAT 
GTACCCACAG CTCGGTCCTG CGTCAACTGG AATCAGTATT ACACCAACTG TACTCAGCTT 
GGGAACAATC CCTTCCAAGG GACGATATCT TTCGACAACA TCGGATTGGC CTGGGTCGCT 
ATATTTTTGG TCATATCACT CGAGGGATGG ACTGACATTA TGTATTATGT TCAAGACGCA 
CACAGTTTTT GGGATTGGAT ATATTTTGTG CTGCTGATTG TGATCGGATC ATTCTTTATG 
ATCAACCTTT GCCTAGTCGT AATTGCCACA CAGTTTTCGG AAACCAAGAA ACGGGAAATG 
GAGCGTATGC GCCAGGAGAG GGCTCGCTTT ACGTCGACCT CGACTTTGGC TTCCAGTACG 
AACAACTCCG AACCAACGAC TTGCTACGCA GAAATCGTCA AGTATATCGC ACATCTGTAC 
CGAAGGCTCA AGCGCCGCAT CATTAAGAAG TTACGACAGT ATAAATATCA ATACCAACAC 
CGGAAGGAAG GGTTGCTACC GCAAACTCCG GAAACCCTAA CATTGTCACC GAACAAGATC 
AAAGTCCACC ATCCAAAGTG TCCGAAAATG CACGCCCTAG CTGTGAGCAC TGTCCACAGG 
AATAATCAGG GTTCCAACAC GCCCTGTGCC ATCATCCCTA GGAACGCCCC GAGCGCTGTT 
GGCACCGAGA AGCAAATGTC CTCACCGGAC ATTTCGGAAA TGGTTTCCGT GGAGAACATC 
AAGAACAATG CTCTGAACAA TTGCACGAGC TATTCGAACT TGGATGACAA ACAGAAAATA 
GTCCTGCTGA AGATTCCAAA TAGTGAGAAC AATCAGTCCT CTTCAAACTC CCTCAGCCCG 
ACGTCCTCAG GTAGACGCAG ATCATCTGTT ATGTTTAATG AATATGTTAT ACATCATACG 
CCTCCAACTA TTCCAGCACC GACAACAGAT AAAAATGTGT ATTGCTTGGA AAAAATGACC 
CAAACCAGCG ATAGTGGAAT TTGGCAGGTC AGCCTACCAC AATCCATCCA AACCATGGCA 
ACGGTTTTCA ACGACTACTC AGACCTTTGC ATGACAGATG CTATGACCTG TCAAGAGCTA 
TTGGCCTTCT CGGTGGCCTT CTCCGCGGCA CTTCCAACGG GACACACAAC ATTGGAATCG 
TTCTACTCGA CGCTCACCAG AAATCGCACG AATCAAAGCA ATAATAAAAA TGCGTCAATT 
ACCAACATTC AAGGATACAG CGGCGATAAT GTCCAGCAGA AATCCCCGCC CAATGTGGCT 
AAAAATCTAT CGCAAAATGT ATCCAACATC AACAATATTA ACAACGAAGA GTTCGCCTGT 
TGCTACGATC TGTACCAAAA CGTGGCATTG GAAGAACCAC CGAAGAAACA CTCAAAGCCC 
TACAGATGCA TAACGGGAAC ATACAGATTT TTCAAAAGAG TACTACACTG TATCAGAGTG 
TACATCAAAA AGTTAGTGGA GCACAAGTAC TTTCAGCAAG GTATATTGCT GGCCATTTTG 
ATCAATACCC TTTCGATGGG CATCGAGTAC CACAATCAAC CGGAAGAGTT GACGGCAATT 
GTCGAGACGA GTAATATTGT GTTCTCTGGC ATCTTTGCTG TTGAGATGGT TCTTAAGGTG 
ATTGCGGAAG GACCGTTCGG ATATGTCGCT AATGGGTTTA ACGTGTTCGA TGGAGTAATT 
GTCATTCTTA GCGTTGTTGA ACTTGGTCAA GCATACCTGG GTGAAGGTCA AGGCAGTTCT 
GGCTTAAGTG TCCTAAGAAC GTTTCGCCTG CTACGTATAC TAAAGTTGGT ACGATTTATG 
CCAAACCTTC GGCGTCAACT GTTCGTGATG TTGAGAACTA TGGACAATGT GGCTATCTTC 
TTCTCCCTGC TGATACTGTT TATTTTCATA TTCAGCATAC TTGGCATGTA CTTGTTTGGA 
GGTAAGTTCT GTAAGTTTGT CGATGAAACT GGACAAGAGA GGGAATGCAC TTGTCCCGAA 
ATAGTCTCCA AGCATCCTCT GTGTGAATGT GATCGCAAAC ATTTCAACAA CATTCTCTGG 
GCCACGGTGA CAGTGTTTCA AATCCTTACG CAAGAAGATT GGAACGTCGT CCTGTTCAAC 
GGGATGGAGA AGACCAGCCA TTGGGCCGCC TTGTACTTTG TAACGCTCAT GACCTTCGGG 
AATTATGTGT TGTTCAATTT GTTGGTTGCT ATTTTGGTTG AAGGGTTTAG CTCAGAGCGA 
AACGAGCGAA GGGAACGAGA GCAGCGGGAA CTCGTGAAGG CCAAACTCAA TGCCGAGTCC 
TTGGCTGCTC AAGAGCAGGG GAGCGAGATC TACGATGATC CCAAAAGCTT TTCAGAGTCA 
ACCACTAGTG ATAGCTATAA TGAGTCCAAG GGGAAATGGT TCAGCGCTGA GGAGCTTCGG 
AAGATAAGGG ACATAAAGTG CAACATACAG AAACAAAGGC TTTTGCAACC CGGGTATGAT 
GACATAAATC TGTCGATTCC AAAGCTAAAC TCGGAGAAAG ATGCTCTCAA TTCTATAGAT 
CTATATTCCA ACCGAAAGAA AAAATGCGAA CCCCTTAAAC TGTACAACAT CCAAGTGACG 
GATCCTCCGA TCATAACGAC AACTGCCGCA ACTCCTCAAG ATTCGCCGAA CGCAACCCTC 
GAAGGAGGTG CCAGTTTCAA AGATTGGGAA AACCTGGACT TTGAACAACT GGAGAAAAGT 
TCCAGCTCAT CGCTACTTCG TCCCCCACCA ATACTGGGAT CTCTGAAAGC TTTGGACGAT 
CGAACGTACT TCGAAGGCAT GCCGGTGCTA AGTGAAATGC GCAGAAAGTC GGATAAGGGT 
ACCGGTTCTC AAGGTGACAG TAAATCAGCG GACCGGATGC AGCAGTCGAC GCAAGACGGA 
TCACGCAAGT CGGAAACTAC GCGGAAGCCT AGTCTGAAGA GTAGGAAGGA TGCCCCACGA 
GAAGGCGAAG TACTCAACAA CGGCAAATGC GTTTCTCGTA GTGGAGGAAT CACGCCACTA 
GATGCCCGCA AAATCGATCC CCTGTCGCAG AGGAGTAACA GGATACAGAA TGATACCCAA 
AGAGACAGAC GAAATTCGTC CCGAAGAAGT TCCGTAAGGC GATCGTCGTC CATCAAGATC 
GACAGTGGAC CCGGAAGCAT CGCCAGCAAT ATAAGCGCTC CATCGTACTC CAAGTACATC 
TACAACGACA AGAATAAGTA TTACATAGAT AGGAAGAGTT CCCTTCGGTT GAGCGACATA 
AAATCTCCGA ACAACGCCCG GCGAATGTCG TCCATAGAGC ACACGTATCA CAAACATTCG 
AGGGTAAGCG TGCACAATCT GGAATTGAGG AAAATCCAAG AAGAACTAAC GAAAAACAAG 
CTGAACAACG CTTCGGTGAC ATCGGTCCAG GATGTACTGC CCGATGCACC TGATACCCGC 
ATCAAGAACA AATCCAAGGG TCGCCTGAAG CAGTTCTTCC GCTACGTCAC CCCGAAGAAT 
TTTGTCGAGG AACGTGATTT GTACACTTTG TATATTTTCC CGGAGGATAA CAGATTCCGC 
CAGATTTGCA GTTGGTTCGT GAATCAGAAA TGGTTCGACA ATGTGATATT GCTGTTCATA 
GCGCTGAATT GTATAACGCT TGCCATGGAG AGACCCAACA TCCCGCCCAC TTGCACCGAG 
AGATATTTCC TGTCCACTGC GAACTATGTT TTTACGGTTG TATTTGCAGT GGAGATGTTC 
ATCAAGGTTG TCGCTACTGG AATGTTCTAC GGTCGAGATG CGTATTTCAC TTCCGGTTGG 
AACATTATGG ATGGTTCGCT TGTCATAATT TCCATCGTCG ATCTTTTGAT GTCCTTGATC 
AGCGAGTCAA GTCCGAGAAT TTTCGGCATA TTGAGGGTCT TTCGATTGTT AAGGTCGCTT 
AGACCTCTAA GGGTAATCAA CAGAGCACCA GGATTGAAGT TGGTGGTTCA AACACTGCTT 
TCATCATTGA GACCCATCGG AAACATAGTG TTGATATGCT GCACATTTTT CATCATCTTT 
GGAATTCTTG GAGTGCAGCT GTTCAAGGGA ACATTCTACT ACTGCGAAGG GGAGAACATC 
AAAGGTGTCA AGAACAAGAC CGAGTGCTTG TCTATCGAAG GAAATGTTTG GGTAAACCGG 
AAGTACAACT TTGACGATCT GGGTAAGGCG TTGATGTCTC TGTTTGTGCT GTCCTCTCGA 
GATGGCTGGG TGAACATTAT GTACACTGGA CTTGATGCGG TCGGTGTTGA CCAACAGCCA 
GTCGTAAACT ACAACGAGTG GCGCTTGCTG TACTTTATTG CGTTTATCCT GCTGGTGGGA 
TTTTTCGTGT TGAACATGTT TGTGGGAGTT GTGGTCGAAA ACTTTCATCG CTGCCGCGAA 
GAGCAGGAAA AGGAAGAGAA AATACGTCGC GCTGCTAAGC GGGCGTTGCA GATGGAGAAG 
AAACGGCGAC GAATGCACGA ACCGCCGTAC TACACCAACT ACTCGCCACT GCGGATGTTC 
GTGCACAACG TGGTAACATC GAAGTACTTC GACCTGGCAA TAGCCGCCGT CATAGGCTTG 
AACGTGGTGA CGATGGCCAT GGAGTACTAC ATGATGCCTC GAGCGTTGGA ATACGCACTA 
AAGATATTCA ACTATTTCTT CACCGCGGTT TTCATACTGG AAGCCATCAT GAAACTGGTG 
GCGCTGGGCT TGAAGATCTA CATGAAAGAC AAATGGAACC AACTGGACGT GGCTATTGTG 
ATTCTGTCCA TTGTGGGCAT CGTGCTGGAG GAGCTCGAGA CCAATATCAT CCCCATAAAT 
CCTACGATCA TACGAGTCAT GCGAGTGCTT CGAATAGCTC GAGTTCTGAA ACTGCTCAAA 
ATGGCGAAAG GTATACGAGC ATTATTGGAC ACGGTGATGC AGGCGTTGCC ACAGGTCGGA 
AACTTGGGAC TATTGTTCTT CCTGTTGTTT TTCATTTTTG CGGCACTGGG AGTGGAGTTG 
TTCGGCAGAC TGGAATGTTC CGAAGAGGTG CCATGCCAGG GATTGGGCGA GCATGCCCAC 
TTCGCAAACT TCGGTATGGC CTTTTTGACG TTGTTCAGAG TTGCGACGGG AGATAACTGG 
AATGGGATCA TGAAGGACAC ACTTCGAGAC GACTGTGACG ATGCTGCCGA TTGTGTTAAG 
AATTGTTGTG TTAGTACTAT AATAGCTCCC ATATTTTTCG TTATATTTGT ACTGATGGCT 
CAGTTTGTAC TGGTCAACGT GGTGGTTGCA GTTCTTATGA AGCATCTTGA AGAAAGCCAC 
AAACAGATGG AAGATGAGTT AGATATTGAA ACAGAGCTGG AAAGGGAATT CGAACGTGAA 
CAAGAATTTG AGGAGGAACA AGCATTGTGT ATGCAACTTA ATGACGACCA AAAGCAATTG 
CAAAAACGGC CTCTCACTAA AGTGTCTTCT TTGCCATCTA ACTTTACTTA TAGCACTCCA 
GTTGCGGAGA AAAAATTAAA CATTCATCGA CGGCAAACTA TTCAATACAT TAATCAGAAT 
CTCAATGTAC AACATTCTTA TACAAACAGC AATTCGTATG ACGAAACTGC TGAAAACGAT 
ATTCCGAATG AAGACGACGA GTCTTTCAAT TCTGTCAATG AAAGCTCTAG TACAAAGAAT 
ACCAACAAGA AAATCCATAT TAACAAATCT TTCTTTAGTA AAAATAACGA AGATAAGCGA 
GCTTCATTGG ATGAATTTAG CAAACCGCAC AAAATTGTGG AGGAATCGGG AAAACCCGAT 
AAACCGAGCT CAACGAAGGG TTCAAACAAA TGGAAAGACA ATCAGTCTGA CCAACCCTCG 
AAAGAAGCGG AAAGCAATAC CAAACTGTCG ACAATCATTT CGTCCAACAA ATCGTTGACG 
AAAAGCAACA AACCCGACTA TCGTCAATTA AGTCTTGATC AAGACGCCAA GCACGACAAC 
GTTACGCTTG CGCCCAACAC TAGTTCTTCT AATTTCTCAA ACGCCTCCAC TGCCCTCAAC 
AACCTCGGTG GCAATAAATC CTCCTTTCTA TCCGTACCCA AACTGCAGCC AAAAAGTCGT 
TCAGGCAGCA CAAAGCAACT GTTCAAACAA GTGGCACTGG ACGAAGATAA TGAGACCAAC 
GAGAGTTCGC TCCTGCTGCC TTCGGTATCG AATGCAGCCG GCTCTGGTGG CGATCACTCG 
TACGATCTTC TGAAGGTGCA GGACGAAAGT TACGATGCTG TGCGAAAATC CGAATCCTGC 
GAGATTCTGA GGATCATTTC CGAGCGACGG AAGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021706157.1
CDS	167..7525
Translation	MLNAYSESQSDLENEQNFGAKQKPRPSVNRQCQDRRSSLVKRSVSAKSNRRPSINSVYNEGQFSESCSMRKKPNKSVGSVSSTSGTTMDSSGSSCSDGDTSSSYGEPNLPYPGFAEYSLKYLSQDTKPRIWCLQLITNPWFERVSMLVILLNCVTLGMYQPCVDDACVTNRCKILQIFDDIIFAFFSLEMTIKIVAMGAWGKGTYLADSWNRLDFFIVLAGALEYCLQVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWEGILRQRCVIKLPDNVSAPDISFYYEFSKEQDYICSKPDDSGMHLCNNLPPYRIGPLVCNASAMPFSNNVPTARSCVNWNQYYTNCTQLGNNPFQGTISFDNIGLAWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERARFTSTSTLASSTNNSEPTTCYAEIVKYIAHLYRRLKRRIIKKLRQYKYQYQHRKEGLLPQTPETLTLSPNKIKVHHPKCPKMHALAVSTVHRNNQGSNTPCAIIPRNAPSAVGTEKQMSSPDISEMVSVENIKNNALNNCTSYSNLDDKQKIVLLKIPNSENNQSSSNSLSPTSSGRRRSSVMFNEYVIHHTPPTIPAPTTDKNVYCLEKMTQTSDSGIWQVSLPQSIQTMATVFNDYSDLCMTDAMTCQELLAFSVAFSAALPTGHTTLESFYSTLTRNRTNQSNNKNASITNIQGYSGDNVQQKSPPNVAKNLSQNVSNINNINNEEFACCYDLYQNVALEEPPKKHSKPYRCITGTYRFFKRVLHCIRVYIKKLVEHKYFQQGILLAILINTLSMGIEYHNQPEELTAIVETSNIVFSGIFAVEMVLKVIAEGPFGYVANGFNVFDGVIVILSVVELGQAYLGEGQGSSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAIFFSLLILFIFIFSILGMYLFGGKFCKFVDETGQERECTCPEIVSKHPLCECDRKHFNNILWATVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVTLMTFGNYVLFNLLVAILVEGFSSERNERREREQRELVKAKLNAESLAAQEQGSEIYDDPKSFSESTTSDSYNESKGKWFSAEELRKIRDIKCNIQKQRLLQPGYDDINLSIPKLNSEKDALNSIDLYSNRKKKCEPLKLYNIQVTDPPIITTTAATPQDSPNATLEGGASFKDWENLDFEQLEKSSSSSLLRPPPILGSLKALDDRTYFEGMPVLSEMRRKSDKGTGSQGDSKSADRMQQSTQDGSRKSETTRKPSLKSRKDAPREGEVLNNGKCVSRSGGITPLDARKIDPLSQRSNRIQNDTQRDRRNSSRRSSVRRSSSIKIDSGPGSIASNISAPSYSKYIYNDKNKYYIDRKSSLRLSDIKSPNNARRMSSIEHTYHKHSRVSVHNLELRKIQEELTKNKLNNASVTSVQDVLPDAPDTRIKNKSKGRLKQFFRYVTPKNFVEERDLYTLYIFPEDNRFRQICSWFVNQKWFDNVILLFIALNCITLAMERPNIPPTCTERYFLSTANYVFTVVFAVEMFIKVVATGMFYGRDAYFTSGWNIMDGSLVIISIVDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVKNKTECLSIEGNVWVNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPVVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPLRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYMMPRALEYALKIFNYFFTAVFILEAIMKLVALGLKIYMKDKWNQLDVAIVILSIVGIVLEELETNIIPINPTIIRVMRVLRIARVLKLLKMAKGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSEEVPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDDCDDAADCVKNCCVSTIIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHKQMEDELDIETELEREFEREQEFEEEQALCMQLNDDQKQLQKRPLTKVSSLPSNFTYSTPVAEKKLNIHRRQTIQYINQNLNVQHSYTNSNSYDETAENDIPNEDDESFNSVNESSSTKNTNKKIHINKSFFSKNNEDKRASLDEFSKPHKIVEESGKPDKPSSTKGSNKWKDNQSDQPSKEAESNTKLSTIISSNKSLTKSNKPDYRQLSLDQDAKHDNVTLAPNTSSSNFSNASTALNNLGGNKSSFLSVPKLQPKSRSGSTKQLFKQVALDEDNETNESSLLLPSVSNAAGSGGDHSYDLLKVQDESYDAVRKSESCEILRIISERRKI

Target ORF information:

RefSeq Version	XM_021850465.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021850465.1

ORF Insert Sequence:

ATGTTGAACG CCTATTCGGA ATCACAATCC GATTTAGAAA ATGAGCAAAA CTTTGGAGCC 
AAACAGAAAC CACGCCCATC CGTCAACCGT CAATGCCAGG ACAGACGCAG CTCGTTGGTC 
AAACGTAGTG TCTCGGCAAA GAGCAATAGA CGGCCGAGCA TCAACAGCGT CTACAACGAG 
GGCCAGTTCA GCGAATCGTG CAGCATGCGA AAGAAACCTA ACAAATCCGT CGGGTCTGTT 
TCATCCACGA GCGGCACCAC GATGGACTCG TCCGGAAGCA GCTGCAGCGA TGGCGACACC 
TCGTCGTCCT ACGGGGAACC GAATCTTCCA TATCCGGGGT TTGCAGAGTA TTCGCTCAAG 
TATCTCAGCC AGGACACGAA ACCGAGAATA TGGTGCTTGC AGTTGATAAC CAATCCATGG 
TTCGAGCGAG TCTCAATGTT GGTGATTTTG CTGAATTGTG TTACATTGGG AATGTATCAA 
CCATGTGTTG ATGACGCCTG TGTGACGAAC CGCTGTAAAA TATTACAAAT ATTCGACGAT 
ATCATATTCG CTTTCTTTTC CCTCGAAATG ACAATCAAGA TAGTGGCGAT GGGTGCCTGG 
GGGAAGGGAA CCTATCTGGC GGATTCGTGG AACCGGCTGG ACTTCTTCAT TGTGCTGGCC 
GGAGCTTTGG AGTACTGCCT TCAGGTGGAA AACCTTAACC TAACCGCAAT CAGAACCATC 
CGTGTACTTA GACCACTGAG GGCAATCAAT CGGATACCGA GTATGCGAAT TCTTGTCATG 
CTATTGCTAG ACACGCTGCC CATGCTCGGT AATGTATTGC TGTTGTGCTT TTTCGTGTTC 
TTCATCTTCG GGATAGTGGG TGTACAGCTT TGGGAGGGCA TCTTGCGGCA GCGTTGCGTC 
ATAAAGCTTC CGGACAACGT GTCGGCTCCA GACATATCAT TTTACTACGA GTTTTCAAAG 
GAGCAGGACT ACATCTGCTC GAAGCCGGAC GACTCGGGAA TGCATCTGTG TAACAACCTT 
CCACCCTACC GGATAGGGCC ATTGGTTTGC AATGCTTCTG CCATGCCATT TTCGAACAAT 
GTACCCACAG CTCGGTCCTG CGTCAACTGG AATCAGTATT ACACCAACTG TACTCAGCTT 
GGGAACAATC CCTTCCAAGG GACGATATCT TTCGACAACA TCGGATTGGC CTGGGTCGCT 
ATATTTTTGG TCATATCACT CGAGGGATGG ACTGACATTA TGTATTATGT TCAAGACGCA 
CACAGTTTTT GGGATTGGAT ATATTTTGTG CTGCTGATTG TGATCGGATC ATTCTTTATG 
ATCAACCTTT GCCTAGTCGT AATTGCCACA CAGTTTTCGG AAACCAAGAA ACGGGAAATG 
GAGCGTATGC GCCAGGAGAG GGCTCGCTTT ACGTCGACCT CGACTTTGGC TTCCAGTACG 
AACAACTCCG AACCAACGAC TTGCTACGCA GAAATCGTCA AGTATATCGC ACATCTGTAC 
CGAAGGCTCA AGCGCCGCAT CATTAAGAAG TTACGACAGT ATAAATATCA ATACCAACAC 
CGGAAGGAAG GGTTGCTACC GCAAACTCCG GAAACCCTAA CATTGTCACC GAACAAGATC 
AAAGTCCACC ATCCAAAGTG TCCGAAAATG CACGCCCTAG CTGTGAGCAC TGTCCACAGG 
AATAATCAGG GTTCCAACAC GCCCTGTGCC ATCATCCCTA GGAACGCCCC GAGCGCTGTT 
GGCACCGAGA AGCAAATGTC CTCACCGGAC ATTTCGGAAA TGGTTTCCGT GGAGAACATC 
AAGAACAATG CTCTGAACAA TTGCACGAGC TATTCGAACT TGGATGACAA ACAGAAAATA 
GTCCTGCTGA AGATTCCAAA TAGTGAGAAC AATCAGTCCT CTTCAAACTC CCTCAGCCCG 
ACGTCCTCAG GTAGACGCAG ATCATCTGTT ATGTTTAATG AATATGTTAT ACATCATACG 
CCTCCAACTA TTCCAGCACC GACAACAGAT AAAAATGTGT ATTGCTTGGA AAAAATGACC 
CAAACCAGCG ATAGTGGAAT TTGGCAGGTC AGCCTACCAC AATCCATCCA AACCATGGCA 
ACGGTTTTCA ACGACTACTC AGACCTTTGC ATGACAGATG CTATGACCTG TCAAGAGCTA 
TTGGCCTTCT CGGTGGCCTT CTCCGCGGCA CTTCCAACGG GACACACAAC ATTGGAATCG 
TTCTACTCGA CGCTCACCAG AAATCGCACG AATCAAAGCA ATAATAAAAA TGCGTCAATT 
ACCAACATTC AAGGATACAG CGGCGATAAT GTCCAGCAGA AATCCCCGCC CAATGTGGCT 
AAAAATCTAT CGCAAAATGT ATCCAACATC AACAATATTA ACAACGAAGA GTTCGCCTGT 
TGCTACGATC TGTACCAAAA CGTGGCATTG GAAGAACCAC CGAAGAAACA CTCAAAGCCC 
TACAGATGCA TAACGGGAAC ATACAGATTT TTCAAAAGAG TACTACACTG TATCAGAGTG 
TACATCAAAA AGTTAGTGGA GCACAAGTAC TTTCAGCAAG GTATATTGCT GGCCATTTTG 
ATCAATACCC TTTCGATGGG CATCGAGTAC CACAATCAAC CGGAAGAGTT GACGGCAATT 
GTCGAGACGA GTAATATTGT GTTCTCTGGC ATCTTTGCTG TTGAGATGGT TCTTAAGGTG 
ATTGCGGAAG GACCGTTCGG ATATGTCGCT AATGGGTTTA ACGTGTTCGA TGGAGTAATT 
GTCATTCTTA GCGTTGTTGA ACTTGGTCAA GCATACCTGG GTGAAGGTCA AGGCAGTTCT 
GGCTTAAGTG TCCTAAGAAC GTTTCGCCTG CTACGTATAC TAAAGTTGGT ACGATTTATG 
CCAAACCTTC GGCGTCAACT GTTCGTGATG TTGAGAACTA TGGACAATGT GGCTATCTTC 
TTCTCCCTGC TGATACTGTT TATTTTCATA TTCAGCATAC TTGGCATGTA CTTGTTTGGA 
GGTAAGTTCT GTAAGTTTGT CGATGAAACT GGACAAGAGA GGGAATGCAC TTGTCCCGAA 
ATAGTCTCCA AGCATCCTCT GTGTGAATGT GATCGCAAAC ATTTCAACAA CATTCTCTGG 
GCCACGGTGA CAGTGTTTCA AATCCTTACG CAAGAAGATT GGAACGTCGT CCTGTTCAAC 
GGGATGGAGA AGACCAGCCA TTGGGCCGCC TTGTACTTTG TAACGCTCAT GACCTTCGGG 
AATTATGTGT TGTTCAATTT GTTGGTTGCT ATTTTGGTTG AAGGGTTTAG CTCAGAGCGA 
AACGAGCGAA GGGAACGAGA GCAGCGGGAA CTCGTGAAGG CCAAACTCAA TGCCGAGTCC 
TTGGCTGCTC AAGAGCAGGG GAGCGAGATC TACGATGATC CCAAAAGCTT TTCAGAGTCA 
ACCACTAGTG ATAGCTATAA TGAGTCCAAG GGGAAATGGT TCAGCGCTGA GGAGCTTCGG 
AAGATAAGGG ACATAAAGTG CAACATACAG AAACAAAGGC TTTTGCAACC CGGGTATGAT 
GACATAAATC TGTCGATTCC AAAGCTAAAC TCGGAGAAAG ATGCTCTCAA TTCTATAGAT 
CTATATTCCA ACCGAAAGAA AAAATGCGAA CCCCTTAAAC TGTACAACAT CCAAGTGACG 
GATCCTCCGA TCATAACGAC AACTGCCGCA ACTCCTCAAG ATTCGCCGAA CGCAACCCTC 
GAAGGAGGTG CCAGTTTCAA AGATTGGGAA AACCTGGACT TTGAACAACT GGAGAAAAGT 
TCCAGCTCAT CGCTACTTCG TCCCCCACCA ATACTGGGAT CTCTGAAAGC TTTGGACGAT 
CGAACGTACT TCGAAGGCAT GCCGGTGCTA AGTGAAATGC GCAGAAAGTC GGATAAGGGT 
ACCGGTTCTC AAGGTGACAG TAAATCAGCG GACCGGATGC AGCAGTCGAC GCAAGACGGA 
TCACGCAAGT CGGAAACTAC GCGGAAGCCT AGTCTGAAGA GTAGGAAGGA TGCCCCACGA 
GAAGGCGAAG TACTCAACAA CGGCAAATGC GTTTCTCGTA GTGGAGGAAT CACGCCACTA 
GATGCCCGCA AAATCGATCC CCTGTCGCAG AGGAGTAACA GGATACAGAA TGATACCCAA 
AGAGACAGAC GAAATTCGTC CCGAAGAAGT TCCGTAAGGC GATCGTCGTC CATCAAGATC 
GACAGTGGAC CCGGAAGCAT CGCCAGCAAT ATAAGCGCTC CATCGTACTC CAAGTACATC 
TACAACGACA AGAATAAGTA TTACATAGAT AGGAAGAGTT CCCTTCGGTT GAGCGACATA 
AAATCTCCGA ACAACGCCCG GCGAATGTCG TCCATAGAGC ACACGTATCA CAAACATTCG 
AGGGTAAGCG TGCACAATCT GGAATTGAGG AAAATCCAAG AAGAACTAAC GAAAAACAAG 
CTGAACAACG CTTCGGTGAC ATCGGTCCAG GATGTACTGC CCGATGCACC TGATACCCGC 
ATCAAGAACA AATCCAAGGG TCGCCTGAAG CAGTTCTTCC GCTACGTCAC CCCGAAGAAT 
TTTGTCGAGG AACGTGATTT GTACACTTTG TATATTTTCC CGGAGGATAA CAGATTCCGC 
CAGATTTGCA GTTGGTTCGT GAATCAGAAA TGGTTCGACA ATGTGATATT GCTGTTCATA 
GCGCTGAATT GTATAACGCT TGCCATGGAG AGACCCAACA TCCCGCCCAC TTGCACCGAG 
AGATATTTCC TGTCCACTGC GAACTATGTT TTTACGGTTG TATTTGCAGT GGAGATGTTC 
ATCAAGGTTG TCGCTACTGG AATGTTCTAC GGTCGAGATG CGTATTTCAC TTCCGGTTGG 
AACATTATGG ATGGTTCGCT TGTCATAATT TCCATCGTCG ATCTTTTGAT GTCCTTGATC 
AGCGAGTCAA GTCCGAGAAT TTTCGGCATA TTGAGGGTCT TTCGATTGTT AAGGTCGCTT 
AGACCTCTAA GGGTAATCAA CAGAGCACCA GGATTGAAGT TGGTGGTTCA AACACTGCTT 
TCATCATTGA GACCCATCGG AAACATAGTG TTGATATGCT GCACATTTTT CATCATCTTT 
GGAATTCTTG GAGTGCAGCT GTTCAAGGGA ACATTCTACT ACTGCGAAGG GGAGAACATC 
AAAGGTGTCA AGAACAAGAC CGAGTGCTTG TCTATCGAAG GAAATGTTTG GGTAAACCGG 
AAGTACAACT TTGACGATCT GGGTAAGGCG TTGATGTCTC TGTTTGTGCT GTCCTCTCGA 
GATGGCTGGG TGAACATTAT GTACACTGGA CTTGATGCGG TCGGTGTTGA CCAACAGCCA 
GTCGTAAACT ACAACGAGTG GCGCTTGCTG TACTTTATTG CGTTTATCCT GCTGGTGGGA 
TTTTTCGTGT TGAACATGTT TGTGGGAGTT GTGGTCGAAA ACTTTCATCG CTGCCGCGAA 
GAGCAGGAAA AGGAAGAGAA AATACGTCGC GCTGCTAAGC GGGCGTTGCA GATGGAGAAG 
AAACGGCGAC GAATGCACGA ACCGCCGTAC TACACCAACT ACTCGCCACT GCGGATGTTC 
GTGCACAACG TGGTAACATC GAAGTACTTC GACCTGGCAA TAGCCGCCGT CATAGGCTTG 
AACGTGGTGA CGATGGCCAT GGAGTACTAC ATGATGCCTC GAGCGTTGGA ATACGCACTA 
AAGATATTCA ACTATTTCTT CACCGCGGTT TTCATACTGG AAGCCATCAT GAAACTGGTG 
GCGCTGGGCT TGAAGATCTA CATGAAAGAC AAATGGAACC AACTGGACGT GGCTATTGTG 
ATTCTGTCCA TTGTGGGCAT CGTGCTGGAG GAGCTCGAGA CCAATATCAT CCCCATAAAT 
CCTACGATCA TACGAGTCAT GCGAGTGCTT CGAATAGCTC GAGTTCTGAA ACTGCTCAAA 
ATGGCGAAAG GTATACGAGC ATTATTGGAC ACGGTGATGC AGGCGTTGCC ACAGGTCGGA 
AACTTGGGAC TATTGTTCTT CCTGTTGTTT TTCATTTTTG CGGCACTGGG AGTGGAGTTG 
TTCGGCAGAC TGGAATGTTC CGAAGAGGTG CCATGCCAGG GATTGGGCGA GCATGCCCAC 
TTCGCAAACT TCGGTATGGC CTTTTTGACG TTGTTCAGAG TTGCGACGGG AGATAACTGG 
AATGGGATCA TGAAGGACAC ACTTCGAGAC GACTGTGACG ATGCTGCCGA TTGTGTTAAG 
AATTGTTGTG TTAGTACTAT AATAGCTCCC ATATTTTTCG TTATATTTGT ACTGATGGCT 
CAGTTTGTAC TGGTCAACGT GGTGGTTGCA GTTCTTATGA AGCATCTTGA AGAAAGCCAC 
AAACAGATGG AAGATGAGTT AGATATTGAA ACAGAGCTGG AAAGGGAATT CGAACGTGAA 
CAAGAATTTG AGGAGGAACA AGCATTGTGT ATGCAACTTA ATGACGACCA AAAGCAATTG 
CAAAAACGGC CTCTCACTAA AGTGTCTTCT TTGCCATCTA ACTTTACTTA TAGCACTCCA 
GTTGCGGAGA AAAAATTAAA CATTCATCGA CGGCAAACTA TTCAATACAT TAATCAGAAT 
CTCAATGTAC AACATTCTTA TACAAACAGC AATTCGTATG ACGAAACTGC TGAAAACGAT 
ATTCCGAATG AAGACGACGA GTCTTTCAAT TCTGTCAATG AAAGCTCTAG TACAAAGAAT 
ACCAACAAGA AAATCCATAT TAACAAATCT TTCTTTAGTA AAAATAACGA AGATAAGCGA 
GCTTCATTGG ATGAATTTAG CAAACCGCAC AAAATTGTGG AGGAATCGGG AAAACCCGAT 
AAACCGAGCT CAACGAAGGG TTCAAACAAA TGGAAAGACA ATCAGTCTGA CCAACCCTCG 
AAAGAAGCGG AAAGCAATAC CAAACTGTCG ACAATCATTT CGTCCAACAA ATCGTTGACG 
AAAAGCAACA AACCCGACTA TCGTCAATTA AGTCTTGATC AAGACGCCAA GCACGACAAC 
GTTACGCTTG CGCCCAACAC TAGTTCTTCT AATTTCTCAA ACGCCTCCAC TGCCCTCAAC 
AACCTCGGTG GCAATAAATC CTCCTTTCTA TCCGTACCCA AACTGCAGCC AAAAAGTCGT 
TCAGGCAGCA CAAAGCAACT GTTCAAACAA GTGGCACTGG ACGAAGATAA TGAGACCAAC 
GAGAGTTCGC TCCTGCTGCC TTCGGTATCG AATGCAGCCG GCTCTGGTGG CGATCACTCG 
TACGATCTTC TGAAGGTGCA GGACGAAAGT TACGATGCTG TGCGAAAATC CGAATCCTGC 
GAGATTCTGA GGATCATTTC CGAGCGACGG AAGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL006849 ( XM_021850466.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021706158.1 Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X4, mRNA.

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CloneID	OAe126303
Clone ID Related Accession (Same CDS sequence)	XM_021850466.1
Accession Version	XM_021850466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACG CCTATTCGGA ATCACAATCC GATTTAGAAA ATGAGCAAAA CTTTGGAGCC 
AAACAGAAAC CACGCCCATC CGTCAACCGT CAATGCCAGG ACAGACGCAG CTCGTTGGTC 
AAACGTAGTG TCTCGGCAAA GAGCAATAGA CGGCCGAGCA TCAACAGCGT CTACAACGAG 
GGCCAGTTCA GCGAATCGTG CAGCATGCGA AAGAAACCTA ACAAATCCGT CGGGTCTGTT 
TCATCCACGA GCGGCACCAC GATGGACTCG TCCGGAAGCA GCTGCAGCGA TGGCGACACC 
TCGTCGTCCT ACGGGGAACC GAATCTTCCA TATCCGGGGT TTGCAGAGTA TTCGCTCAAG 
TATCTCAGCC AGGACACGAA ACCGAGAATA TGGTGCTTGC AGTTGATAAC CAATCCATGG 
TTCGAGCGAG TCTCAATGTT GGTGATTTTG CTGAATTGTG TTACATTGGG AATGTATCAA 
CCATGTGTTG ATGACGCCTG TGTGACGAAC CGCTGTAAAA TATTACAAAT ATTCGACGAT 
ATCATATTCG CTTTCTTTTC CCTCGAAATG ACAATCAAGA TAGTGGCGAT GGGTGCCTGG 
GGGAAGGGAA CCTATCTGGC GGATTCGTGG AACCGGCTGG ACTTCTTCAT TGTGCTGGCC 
GGAGCTTTGG AGTACTGCCT TCAGGTGGAA AACCTTAACC TAACCGCAAT CAGAACCATC 
CGTGTACTTA GACCACTGAG GGCAATCAAT CGGATACCGA GTATGCGAAT TCTTGTCATG 
CTATTGCTAG ACACGCTGCC CATGCTCGGT AATGTATTGC TGTTGTGCTT TTTCGTGTTC 
TTCATCTTCG GGATAGTGGG TGTACAGCTT TGGGAGGGCA TCTTGCGGCA GCGTTGCGTC 
ATAAAGCTTC CGGACAACGT GTCGGCTCCA GACACATTTA CACAACGTAA CCTTTACTAT 
AGAATTGTGA CCCAAAAGCA AAGGAAATCT GACATATCAT TTTACTACGA GTTTTCAAAG 
GAGCAGGACT ACATCTGCTC GAAGCCGGAC GACTCGGGAA TGCATCTGTG TAACAACCTT 
CCACCCTACC GGATAGGGCC ATTGGTTTGC AATGCTTCTG CCATGCCATT TTCGAACAAT 
GTACCCACAG CTCGGTCCTG CGTCAACTGG AATCAGTATT ACACCAACTG TACTCAGCTT 
GGGAACAATC CCTTCCAAGG GACGATATCT TTCGACAACA TCGGATTGGC CTGGGTCGCT 
ATATTTTTGG TCATATCACT CGAGGGATGG ACTGACATTA TGTATTATGT TCAAGACGCA 
CACAGTTTTT GGGATTGGAT ATATTTTGTG CTGCTGATTG TGATCGGATC ATTCTTTATG 
ATCAACCTTT GCCTAGTCGT AATTGCCACA CAGTTTTCGG AAACCAAGAA ACGGGAAATG 
GAGCGTATGC GCCAGGAGAG GGCTCGCTTT ACGTCGACCT CGACTTTGGC TTCCAGTACG 
AACAACTCCG AACCAACGAC TTGCTACGCA GAAATCGTCA AGTATATCGC ACATCTGTAC 
CGAAGGCTCA AGCGCCGCAT CATTAAGAAG TTACGACAGT ATAAATATCA ATACCAACAC 
CGGAAGGAAG GGTTGCTACC GCAAACTCCG GAAACCCTAA CATTGTCACC GAACAAGATC 
AAAGTCCACC ATCCAAAGTG TCCGAAAATG CACGCCCTAG CTGTGAGCAC TGTCCACAGG 
AATAATCAGG GTTCCAACAC GCCCTGTGCC ATCATCCCTA GGAACGCCCC GAGCGCTGTT 
GGCACCGAGA AGCAAATGTC CTCACCGGAC ATTTCGGAAA TGGTTTCCGT GGAGAACATC 
AAGAACAATG CTCTGAACAA TTGCACGAGC TATTCGAACT TGGATGACAA ACAGAAAATA 
GTCCTGCTGA AGATTCCAAA TAGTGAGAAC AATCAGTCCT CTTCAAACTC CCTCAGCCCG 
ACGTCCTCAG CACCGACAAC AGATAAAAAT GTGTATTGCT TGGAAAAAAT GACCCAAACC 
AGCGATAGTG GAATTTGGCA GGTCAGCCTA CCACAATCCA TCCAAACCAT GGCAACGGTT 
TTCAACGACT ACTCAGACCT TTGCATGACA GATGCTATGA CCTGTCAAGA GCTATTGGCC 
TTCTCGGTGG CCTTCTCCGC GGCACTTCCA ACGGGACACA CAACATTGGA ATCGTTCTAC 
TCGACGCTCA CCAGAAATCG CACGAATCAA AGCAATAATA AAAATGCGTC AATTACCAAC 
ATTCAAGGAT ACAGCGGCGA TAATGTCCAG CAGAAATCCC CGCCCAATGT GGCTAAAAAT 
CTATCGCAAA ATGTATCCAA CATCAACAAT ATTAACAACG AAGAGTTCGC CTGTTGCTAC 
GATCTGTACC AAAACGTGGC ATTGGAAGAA CCACCGAAGA AACACTCAAA GCCCTACAGA 
TGCATAACGG GAACATACAG ATTTTTCAAA AGAGTACTAC ACTGTATCAG AGTGTACATC 
AAAAAGTTAG TGGAGCACAA GTACTTTCAG CAAGGTATAT TGCTGGCCAT TTTGATCAAT 
ACCCTTTCGA TGGGCATCGA GTACCACAAT CAACCGGAAG AGTTGACGGC AATTGTCGAG 
ACGAGTAATA TTGTGTTCTC TGGCATCTTT GCTGTTGAGA TGGTTCTTAA GGTGATTGCG 
GAAGGACCGT TCGGATATGT CGCTAATGGG TTTAACGTGT TCGATGGAGT AATTGTCATT 
CTTAGCGTTG TTGAACTTGG TCAAGCATAC CTGGGTGAAG GTCAAGGCAG TTCTGGCTTA 
AGTGTCCTAA GAACGTTTCG CCTGCTACGT ATACTAAAGT TGGTACGATT TATGCCAAAC 
CTTCGGCGTC AACTGTTCGT GATGTTGAGA ACTATGGACA ATGTGGCTAT CTTCTTCTCC 
CTGCTGATAC TGTTTATTTT CATATTCAGC ATACTTGGCA TGTACTTGTT TGGAGGTAAG 
TTCTGTAAGT TTGTCGATGA AACTGGACAA GAGAGGGAAT GCACTTGTCC CGAAATAGTC 
TCCAAGCATC CTCTGTGTGA ATGTGATCGC AAACATTTCA ACAACATTCT CTGGGCCACG 
GTGACAGTGT TTCAAATCCT TACGCAAGAA GATTGGAACG TCGTCCTGTT CAACGGGATG 
GAGAAGACCA GCCATTGGGC CGCCTTGTAC TTTGTAACGC TCATGACCTT CGGGAATTAT 
GTGTTGTTCA ATTTGTTGGT TGCTATTTTG GTTGAAGGGT TTAGCTCAGA GCGAAACGAG 
CGAAGGGAAC GAGAGCAGCG GGAACTCGTG AAGGCCAAAC TCAATGCCGA GTCCTTGGCT 
GCTCAAGAGC AGGGGAGCGA GATCTACGAT GATCCCAAAA GCTTTTCAGA GTCAACCACT 
AGTGATAGCT ATAATGAGTC CAAGGGGAAA TGGTTCAGCG CTGAGGAGCT TCGGAAGATA 
AGGGACATAA AGTGCAACAT ACAGAAACAA AGGCTTTTGC AACCCGGGTA TGATGACATA 
AATCTGTCGA TTCCAAAGCT AAACTCGGAG AAAGATGCTC TCAATTCTAT AGATCTATAT 
TCCAACCGAA AGAAAAAATG CGAACCCCTT AAACTGTACA ACATCCAAGT GACGGATCCT 
CCGATCATAA CGACAACTGC CGCAACTCCT CAAGATTCGC CGAACGCAAC CCTCGAAGGA 
GGTGCCAGTT TCAAAGATTG GGAAAACCTG GACTTTGAAC AACTGGAGAA AAGTTCCAGC 
TCATCGCTAC TTCGTCCCCC ACCAATACTG GGATCTCTGA AAGCTTTGGA CGATCGAACG 
TACTTCGAAG GCATGCCGGT GCTAAGTGAA ATGCGCAGAA AGTCGGATAA GGGTACCGGT 
TCTCAAGGTG ACAGTAAATC AGCGGACCGG ATGCAGCAGT CGACGCAAGA CGGATCACGC 
AAGTCGGAAA CTACGCGGAA GCCTAGTCTG AAGAGTAGGA AGGATGCCCC ACGAGAAGGC 
GAAGTACTCA ACAACGGCAA ATGCGTTTCT CGTAGTGGAG GAATCACGCC ACTAGATGCC 
CGCAAAATCG ATCCCCTGTC GCAGAGGAGT AACAGGATAC AGAATGATAC CCAAAGAGAC 
AGACGAAATT CGTCCCGAAG AAGTTCCGTA AGGCGATCGT CGTCCATCAA GATCGACAGT 
GGACCCGGAA GCATCGCCAG CAATATAAGC GCTCCATCGT ACTCCAAGTA CATCTACAAC 
GACAAGAATA AGTATTACAT AGATAGGAAG AGTTCCCTTC GGTTGAGCGA CATAAAATCT 
CCGAACAACG CCCGGCGAAT GTCGTCCATA GAGCACACGT ATCACAAACA TTCGAGGGTA 
AGCGTGCACA ATCTGGAATT GAGGAAAATC CAAGAAGAAC TAACGAAAAA CAAGCTGAAC 
AACGCTTCGG TGACATCGGT CCAGGATGTA CTGCCCGATG CACCTGATAC CCGCATCAAG 
AACAAATCCA AGGGTCGCCT GAAGCAGTTC TTCCGCTACG TCACCCCGAA GAATTTTGTC 
GAGGAACGTG ATTTGTACAC TTTGTATATT TTCCCGGAGG ATAACAGATT CCGCCAGATT 
TGCAGTTGGT TCGTGAATCA GAAATGGTTC GACAATGTGA TATTGCTGTT CATAGCGCTG 
AATTGTATAA CGCTTGCCAT GGAGAGACCC AACATCCCGC CCACTTGCAC CGAGAGATAT 
TTCCTGTCCA CTGCGAACTA TGTTTTTACG GTTGTATTTG CAGTGGAGAT GTTCATCAAG 
GTTGTCGCTA CTGGAATGTT CTACGGTCGA GATGCGTATT TCACTTCCGG TTGGAACATT 
ATGGATGGTT CGCTTGTCAT AATTTCCATC GTCGATCTTT TGATGTCCTT GATCAGCGAG 
TCAAGTCCGA GAATTTTCGG CATATTGAGG GTCTTTCGAT TGTTAAGGTC GCTTAGACCT 
CTAAGGGTAA TCAACAGAGC ACCAGGATTG AAGTTGGTGG TTCAAACACT GCTTTCATCA 
TTGAGACCCA TCGGAAACAT AGTGTTGATA TGCTGCACAT TTTTCATCAT CTTTGGAATT 
CTTGGAGTGC AGCTGTTCAA GGGAACATTC TACTACTGCG AAGGGGAGAA CATCAAAGGT 
GTCAAGAACA AGACCGAGTG CTTGTCTATC GAAGGAAATG TTTGGGTAAA CCGGAAGTAC 
AACTTTGACG ATCTGGGTAA GGCGTTGATG TCTCTGTTTG TGCTGTCCTC TCGAGATGGC 
TGGGTGAACA TTATGTACAC TGGACTTGAT GCGGTCGGTG TTGACCAACA GCCAGTCGTA 
AACTACAACG AGTGGCGCTT GCTGTACTTT ATTGCGTTTA TCCTGCTGGT GGGATTTTTC 
GTGTTGAACA TGTTTGTGGG AGTTGTGGTC GAAAACTTTC ATCGCTGCCG CGAAGAGCAG 
GAAAAGGAAG AGAAAATACG TCGCGCTGCT AAGCGGGCGT TGCAGATGGA GAAGAAACGG 
CGACGAATGC ACGAACCGCC GTACTACACC AACTACTCGC CACTGCGGAT GTTCGTGCAC 
AACGTGGTAA CATCGAAGTA CTTCGACCTG GCAATAGCCG CCGTCATAGG CTTGAACGTG 
GTGACGATGG CCATGGAGTA CTACATGATG CCTCGAGCGT TGGAATACGC ACTAAAGATA 
TTCAACTATT TCTTCACCGC GGTTTTCATA CTGGAAGCCA TCATGAAACT GGTGGCGCTG 
GGCTTGAAGA TCTACATGAA AGACAAATGG AACCAACTGG ACGTGGCTAT TGTGATTCTG 
TCCATTGTGG GCATCGTGCT GGAGGAGCTC GAGACCAATA TCATCCCCAT AAATCCTACG 
ATCATACGAG TCATGCGAGT GCTTCGAATA GCTCGAGTTC TGAAACTGCT CAAAATGGCG 
AAAGGTATAC GAGCATTATT GGACACGGTG ATGCAGGCGT TGCCACAGGT CGGAAACTTG 
GGACTATTGT TCTTCCTGTT GTTTTTCATT TTTGCGGCAC TGGGAGTGGA GTTGTTCGGC 
AGACTGGAAT GTTCCGAAGA GGTGCCATGC CAGGGATTGG GCGAGCATGC CCACTTCGCA 
AACTTCGGTA TGGCCTTTTT GACGTTGTTC AGAGTTGCGA CGGGAGATAA CTGGAATGGG 
ATCATGAAGG ACACACTTCG AGACGACTGT GACGATGCTG CCGATTGTGT TAAGAATTGT 
TGTGTTAGTA CTATAATAGC TCCCATATTT TTCGTTATAT TTGTACTGAT GGCTCAGTTT 
GTACTGGTCA ACGTGGTGGT TGCAGTTCTT ATGAAGCATC TTGAAGAAAG CCACAAACAG 
ATGGAAGATG AGTTAGATAT TGAAACAGAG CTGGAAAGGG AATTCGAACG TGAACAAGAA 
TTTGAGGAGG AACAAGCATT GTGTATGCAA CTTAATGACG ACCAAAAGCA ATTGCAAAAA 
CGGCCTCTCA CTAAAGTGTC TTCTTTGCCA TCTAACTTTA CTTATAGCAC TCCAGTTGCG 
GAGAAAAAAT TAAACATTCA TCGACGGCAA ACTATTCAAT ACATTAATCA GAATCTCAAT 
GTACAACATT CTTATACAAA CAGCAATTCG TATGACGAAA CTGCTGAAAA CGATATTCCG 
AATGAAGACG ACGAGTCTTT CAATTCTGTC AATGAAAGCT CTAGTACAAA GAATACCAAC 
AAGAAAATCC ATATTAACAA ATCTTTCTTT AGTAAAAATA ACGAAGATAA GCGAGCTTCA 
TTGGATGAAT TTAGCAAACC GCACAAAATT GTGGAGGAAT CGGGAAAACC CGATAAACCG 
AGCTCAACGA AGGGTTCAAA CAAATGGAAA GACAATCAGT CTGACCAACC CTCGAAAGAA 
GCGGAAAGCA ATACCAAACT GTCGACAATC ATTTCGTCCA ACAAATCGTT GACGAAAAGC 
AACAAACCCG ACTATCGTCA ATTAAGTCTT GATCAAGACG CCAAGCACGA CAACGTTACG 
CTTGCGCCCA ACACTAGTTC TTCTAATTTC TCAAACGCCT CCACTGCCCT CAACAACCTC 
GGTGGCAATA AATCCTCCTT TCTATCCGTA CCCAAACTGC AGCCAAAAAG TCGTTCAGGC 
AGCACAAAGC AACTGTTCAA ACAAGTGGCA CTGGACGAAG ATAATGAGAC CAACGAGAGT 
TCGCTCCTGC TGCCTTCGGT ATCGAATGCA GCCGGCTCTG GTGGCGATCA CTCGTACGAT 
CTTCTGAAGG TGCAGGACGA AAGTTACGAT GCTGTGCGAA AATCCGAATC CTGCGAGATT 
CTGAGGATCA TTTCCGAGCG ACGGAAGATC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021706158.1
CDS	167..7519
Translation	MLNAYSESQSDLENEQNFGAKQKPRPSVNRQCQDRRSSLVKRSVSAKSNRRPSINSVYNEGQFSESCSMRKKPNKSVGSVSSTSGTTMDSSGSSCSDGDTSSSYGEPNLPYPGFAEYSLKYLSQDTKPRIWCLQLITNPWFERVSMLVILLNCVTLGMYQPCVDDACVTNRCKILQIFDDIIFAFFSLEMTIKIVAMGAWGKGTYLADSWNRLDFFIVLAGALEYCLQVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWEGILRQRCVIKLPDNVSAPDTFTQRNLYYRIVTQKQRKSDISFYYEFSKEQDYICSKPDDSGMHLCNNLPPYRIGPLVCNASAMPFSNNVPTARSCVNWNQYYTNCTQLGNNPFQGTISFDNIGLAWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERARFTSTSTLASSTNNSEPTTCYAEIVKYIAHLYRRLKRRIIKKLRQYKYQYQHRKEGLLPQTPETLTLSPNKIKVHHPKCPKMHALAVSTVHRNNQGSNTPCAIIPRNAPSAVGTEKQMSSPDISEMVSVENIKNNALNNCTSYSNLDDKQKIVLLKIPNSENNQSSSNSLSPTSSAPTTDKNVYCLEKMTQTSDSGIWQVSLPQSIQTMATVFNDYSDLCMTDAMTCQELLAFSVAFSAALPTGHTTLESFYSTLTRNRTNQSNNKNASITNIQGYSGDNVQQKSPPNVAKNLSQNVSNINNINNEEFACCYDLYQNVALEEPPKKHSKPYRCITGTYRFFKRVLHCIRVYIKKLVEHKYFQQGILLAILINTLSMGIEYHNQPEELTAIVETSNIVFSGIFAVEMVLKVIAEGPFGYVANGFNVFDGVIVILSVVELGQAYLGEGQGSSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAIFFSLLILFIFIFSILGMYLFGGKFCKFVDETGQERECTCPEIVSKHPLCECDRKHFNNILWATVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVTLMTFGNYVLFNLLVAILVEGFSSERNERREREQRELVKAKLNAESLAAQEQGSEIYDDPKSFSESTTSDSYNESKGKWFSAEELRKIRDIKCNIQKQRLLQPGYDDINLSIPKLNSEKDALNSIDLYSNRKKKCEPLKLYNIQVTDPPIITTTAATPQDSPNATLEGGASFKDWENLDFEQLEKSSSSSLLRPPPILGSLKALDDRTYFEGMPVLSEMRRKSDKGTGSQGDSKSADRMQQSTQDGSRKSETTRKPSLKSRKDAPREGEVLNNGKCVSRSGGITPLDARKIDPLSQRSNRIQNDTQRDRRNSSRRSSVRRSSSIKIDSGPGSIASNISAPSYSKYIYNDKNKYYIDRKSSLRLSDIKSPNNARRMSSIEHTYHKHSRVSVHNLELRKIQEELTKNKLNNASVTSVQDVLPDAPDTRIKNKSKGRLKQFFRYVTPKNFVEERDLYTLYIFPEDNRFRQICSWFVNQKWFDNVILLFIALNCITLAMERPNIPPTCTERYFLSTANYVFTVVFAVEMFIKVVATGMFYGRDAYFTSGWNIMDGSLVIISIVDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVKNKTECLSIEGNVWVNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPVVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPLRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYMMPRALEYALKIFNYFFTAVFILEAIMKLVALGLKIYMKDKWNQLDVAIVILSIVGIVLEELETNIIPINPTIIRVMRVLRIARVLKLLKMAKGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSEEVPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDDCDDAADCVKNCCVSTIIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHKQMEDELDIETELEREFEREQEFEEEQALCMQLNDDQKQLQKRPLTKVSSLPSNFTYSTPVAEKKLNIHRRQTIQYINQNLNVQHSYTNSNSYDETAENDIPNEDDESFNSVNESSSTKNTNKKIHINKSFFSKNNEDKRASLDEFSKPHKIVEESGKPDKPSSTKGSNKWKDNQSDQPSKEAESNTKLSTIISSNKSLTKSNKPDYRQLSLDQDAKHDNVTLAPNTSSSNFSNASTALNNLGGNKSSFLSVPKLQPKSRSGSTKQLFKQVALDEDNETNESSLLLPSVSNAAGSGGDHSYDLLKVQDESYDAVRKSESCEILRIISERRKI

Target ORF information:

RefSeq Version	XM_021850466.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021850466.1

ORF Insert Sequence:

ATGTTGAACG CCTATTCGGA ATCACAATCC GATTTAGAAA ATGAGCAAAA CTTTGGAGCC 
AAACAGAAAC CACGCCCATC CGTCAACCGT CAATGCCAGG ACAGACGCAG CTCGTTGGTC 
AAACGTAGTG TCTCGGCAAA GAGCAATAGA CGGCCGAGCA TCAACAGCGT CTACAACGAG 
GGCCAGTTCA GCGAATCGTG CAGCATGCGA AAGAAACCTA ACAAATCCGT CGGGTCTGTT 
TCATCCACGA GCGGCACCAC GATGGACTCG TCCGGAAGCA GCTGCAGCGA TGGCGACACC 
TCGTCGTCCT ACGGGGAACC GAATCTTCCA TATCCGGGGT TTGCAGAGTA TTCGCTCAAG 
TATCTCAGCC AGGACACGAA ACCGAGAATA TGGTGCTTGC AGTTGATAAC CAATCCATGG 
TTCGAGCGAG TCTCAATGTT GGTGATTTTG CTGAATTGTG TTACATTGGG AATGTATCAA 
CCATGTGTTG ATGACGCCTG TGTGACGAAC CGCTGTAAAA TATTACAAAT ATTCGACGAT 
ATCATATTCG CTTTCTTTTC CCTCGAAATG ACAATCAAGA TAGTGGCGAT GGGTGCCTGG 
GGGAAGGGAA CCTATCTGGC GGATTCGTGG AACCGGCTGG ACTTCTTCAT TGTGCTGGCC 
GGAGCTTTGG AGTACTGCCT TCAGGTGGAA AACCTTAACC TAACCGCAAT CAGAACCATC 
CGTGTACTTA GACCACTGAG GGCAATCAAT CGGATACCGA GTATGCGAAT TCTTGTCATG 
CTATTGCTAG ACACGCTGCC CATGCTCGGT AATGTATTGC TGTTGTGCTT TTTCGTGTTC 
TTCATCTTCG GGATAGTGGG TGTACAGCTT TGGGAGGGCA TCTTGCGGCA GCGTTGCGTC 
ATAAAGCTTC CGGACAACGT GTCGGCTCCA GACACATTTA CACAACGTAA CCTTTACTAT 
AGAATTGTGA CCCAAAAGCA AAGGAAATCT GACATATCAT TTTACTACGA GTTTTCAAAG 
GAGCAGGACT ACATCTGCTC GAAGCCGGAC GACTCGGGAA TGCATCTGTG TAACAACCTT 
CCACCCTACC GGATAGGGCC ATTGGTTTGC AATGCTTCTG CCATGCCATT TTCGAACAAT 
GTACCCACAG CTCGGTCCTG CGTCAACTGG AATCAGTATT ACACCAACTG TACTCAGCTT 
GGGAACAATC CCTTCCAAGG GACGATATCT TTCGACAACA TCGGATTGGC CTGGGTCGCT 
ATATTTTTGG TCATATCACT CGAGGGATGG ACTGACATTA TGTATTATGT TCAAGACGCA 
CACAGTTTTT GGGATTGGAT ATATTTTGTG CTGCTGATTG TGATCGGATC ATTCTTTATG 
ATCAACCTTT GCCTAGTCGT AATTGCCACA CAGTTTTCGG AAACCAAGAA ACGGGAAATG 
GAGCGTATGC GCCAGGAGAG GGCTCGCTTT ACGTCGACCT CGACTTTGGC TTCCAGTACG 
AACAACTCCG AACCAACGAC TTGCTACGCA GAAATCGTCA AGTATATCGC ACATCTGTAC 
CGAAGGCTCA AGCGCCGCAT CATTAAGAAG TTACGACAGT ATAAATATCA ATACCAACAC 
CGGAAGGAAG GGTTGCTACC GCAAACTCCG GAAACCCTAA CATTGTCACC GAACAAGATC 
AAAGTCCACC ATCCAAAGTG TCCGAAAATG CACGCCCTAG CTGTGAGCAC TGTCCACAGG 
AATAATCAGG GTTCCAACAC GCCCTGTGCC ATCATCCCTA GGAACGCCCC GAGCGCTGTT 
GGCACCGAGA AGCAAATGTC CTCACCGGAC ATTTCGGAAA TGGTTTCCGT GGAGAACATC 
AAGAACAATG CTCTGAACAA TTGCACGAGC TATTCGAACT TGGATGACAA ACAGAAAATA 
GTCCTGCTGA AGATTCCAAA TAGTGAGAAC AATCAGTCCT CTTCAAACTC CCTCAGCCCG 
ACGTCCTCAG CACCGACAAC AGATAAAAAT GTGTATTGCT TGGAAAAAAT GACCCAAACC 
AGCGATAGTG GAATTTGGCA GGTCAGCCTA CCACAATCCA TCCAAACCAT GGCAACGGTT 
TTCAACGACT ACTCAGACCT TTGCATGACA GATGCTATGA CCTGTCAAGA GCTATTGGCC 
TTCTCGGTGG CCTTCTCCGC GGCACTTCCA ACGGGACACA CAACATTGGA ATCGTTCTAC 
TCGACGCTCA CCAGAAATCG CACGAATCAA AGCAATAATA AAAATGCGTC AATTACCAAC 
ATTCAAGGAT ACAGCGGCGA TAATGTCCAG CAGAAATCCC CGCCCAATGT GGCTAAAAAT 
CTATCGCAAA ATGTATCCAA CATCAACAAT ATTAACAACG AAGAGTTCGC CTGTTGCTAC 
GATCTGTACC AAAACGTGGC ATTGGAAGAA CCACCGAAGA AACACTCAAA GCCCTACAGA 
TGCATAACGG GAACATACAG ATTTTTCAAA AGAGTACTAC ACTGTATCAG AGTGTACATC 
AAAAAGTTAG TGGAGCACAA GTACTTTCAG CAAGGTATAT TGCTGGCCAT TTTGATCAAT 
ACCCTTTCGA TGGGCATCGA GTACCACAAT CAACCGGAAG AGTTGACGGC AATTGTCGAG 
ACGAGTAATA TTGTGTTCTC TGGCATCTTT GCTGTTGAGA TGGTTCTTAA GGTGATTGCG 
GAAGGACCGT TCGGATATGT CGCTAATGGG TTTAACGTGT TCGATGGAGT AATTGTCATT 
CTTAGCGTTG TTGAACTTGG TCAAGCATAC CTGGGTGAAG GTCAAGGCAG TTCTGGCTTA 
AGTGTCCTAA GAACGTTTCG CCTGCTACGT ATACTAAAGT TGGTACGATT TATGCCAAAC 
CTTCGGCGTC AACTGTTCGT GATGTTGAGA ACTATGGACA ATGTGGCTAT CTTCTTCTCC 
CTGCTGATAC TGTTTATTTT CATATTCAGC ATACTTGGCA TGTACTTGTT TGGAGGTAAG 
TTCTGTAAGT TTGTCGATGA AACTGGACAA GAGAGGGAAT GCACTTGTCC CGAAATAGTC 
TCCAAGCATC CTCTGTGTGA ATGTGATCGC AAACATTTCA ACAACATTCT CTGGGCCACG 
GTGACAGTGT TTCAAATCCT TACGCAAGAA GATTGGAACG TCGTCCTGTT CAACGGGATG 
GAGAAGACCA GCCATTGGGC CGCCTTGTAC TTTGTAACGC TCATGACCTT CGGGAATTAT 
GTGTTGTTCA ATTTGTTGGT TGCTATTTTG GTTGAAGGGT TTAGCTCAGA GCGAAACGAG 
CGAAGGGAAC GAGAGCAGCG GGAACTCGTG AAGGCCAAAC TCAATGCCGA GTCCTTGGCT 
GCTCAAGAGC AGGGGAGCGA GATCTACGAT GATCCCAAAA GCTTTTCAGA GTCAACCACT 
AGTGATAGCT ATAATGAGTC CAAGGGGAAA TGGTTCAGCG CTGAGGAGCT TCGGAAGATA 
AGGGACATAA AGTGCAACAT ACAGAAACAA AGGCTTTTGC AACCCGGGTA TGATGACATA 
AATCTGTCGA TTCCAAAGCT AAACTCGGAG AAAGATGCTC TCAATTCTAT AGATCTATAT 
TCCAACCGAA AGAAAAAATG CGAACCCCTT AAACTGTACA ACATCCAAGT GACGGATCCT 
CCGATCATAA CGACAACTGC CGCAACTCCT CAAGATTCGC CGAACGCAAC CCTCGAAGGA 
GGTGCCAGTT TCAAAGATTG GGAAAACCTG GACTTTGAAC AACTGGAGAA AAGTTCCAGC 
TCATCGCTAC TTCGTCCCCC ACCAATACTG GGATCTCTGA AAGCTTTGGA CGATCGAACG 
TACTTCGAAG GCATGCCGGT GCTAAGTGAA ATGCGCAGAA AGTCGGATAA GGGTACCGGT 
TCTCAAGGTG ACAGTAAATC AGCGGACCGG ATGCAGCAGT CGACGCAAGA CGGATCACGC 
AAGTCGGAAA CTACGCGGAA GCCTAGTCTG AAGAGTAGGA AGGATGCCCC ACGAGAAGGC 
GAAGTACTCA ACAACGGCAA ATGCGTTTCT CGTAGTGGAG GAATCACGCC ACTAGATGCC 
CGCAAAATCG ATCCCCTGTC GCAGAGGAGT AACAGGATAC AGAATGATAC CCAAAGAGAC 
AGACGAAATT CGTCCCGAAG AAGTTCCGTA AGGCGATCGT CGTCCATCAA GATCGACAGT 
GGACCCGGAA GCATCGCCAG CAATATAAGC GCTCCATCGT ACTCCAAGTA CATCTACAAC 
GACAAGAATA AGTATTACAT AGATAGGAAG AGTTCCCTTC GGTTGAGCGA CATAAAATCT 
CCGAACAACG CCCGGCGAAT GTCGTCCATA GAGCACACGT ATCACAAACA TTCGAGGGTA 
AGCGTGCACA ATCTGGAATT GAGGAAAATC CAAGAAGAAC TAACGAAAAA CAAGCTGAAC 
AACGCTTCGG TGACATCGGT CCAGGATGTA CTGCCCGATG CACCTGATAC CCGCATCAAG 
AACAAATCCA AGGGTCGCCT GAAGCAGTTC TTCCGCTACG TCACCCCGAA GAATTTTGTC 
GAGGAACGTG ATTTGTACAC TTTGTATATT TTCCCGGAGG ATAACAGATT CCGCCAGATT 
TGCAGTTGGT TCGTGAATCA GAAATGGTTC GACAATGTGA TATTGCTGTT CATAGCGCTG 
AATTGTATAA CGCTTGCCAT GGAGAGACCC AACATCCCGC CCACTTGCAC CGAGAGATAT 
TTCCTGTCCA CTGCGAACTA TGTTTTTACG GTTGTATTTG CAGTGGAGAT GTTCATCAAG 
GTTGTCGCTA CTGGAATGTT CTACGGTCGA GATGCGTATT TCACTTCCGG TTGGAACATT 
ATGGATGGTT CGCTTGTCAT AATTTCCATC GTCGATCTTT TGATGTCCTT GATCAGCGAG 
TCAAGTCCGA GAATTTTCGG CATATTGAGG GTCTTTCGAT TGTTAAGGTC GCTTAGACCT 
CTAAGGGTAA TCAACAGAGC ACCAGGATTG AAGTTGGTGG TTCAAACACT GCTTTCATCA 
TTGAGACCCA TCGGAAACAT AGTGTTGATA TGCTGCACAT TTTTCATCAT CTTTGGAATT 
CTTGGAGTGC AGCTGTTCAA GGGAACATTC TACTACTGCG AAGGGGAGAA CATCAAAGGT 
GTCAAGAACA AGACCGAGTG CTTGTCTATC GAAGGAAATG TTTGGGTAAA CCGGAAGTAC 
AACTTTGACG ATCTGGGTAA GGCGTTGATG TCTCTGTTTG TGCTGTCCTC TCGAGATGGC 
TGGGTGAACA TTATGTACAC TGGACTTGAT GCGGTCGGTG TTGACCAACA GCCAGTCGTA 
AACTACAACG AGTGGCGCTT GCTGTACTTT ATTGCGTTTA TCCTGCTGGT GGGATTTTTC 
GTGTTGAACA TGTTTGTGGG AGTTGTGGTC GAAAACTTTC ATCGCTGCCG CGAAGAGCAG 
GAAAAGGAAG AGAAAATACG TCGCGCTGCT AAGCGGGCGT TGCAGATGGA GAAGAAACGG 
CGACGAATGC ACGAACCGCC GTACTACACC AACTACTCGC CACTGCGGAT GTTCGTGCAC 
AACGTGGTAA CATCGAAGTA CTTCGACCTG GCAATAGCCG CCGTCATAGG CTTGAACGTG 
GTGACGATGG CCATGGAGTA CTACATGATG CCTCGAGCGT TGGAATACGC ACTAAAGATA 
TTCAACTATT TCTTCACCGC GGTTTTCATA CTGGAAGCCA TCATGAAACT GGTGGCGCTG 
GGCTTGAAGA TCTACATGAA AGACAAATGG AACCAACTGG ACGTGGCTAT TGTGATTCTG 
TCCATTGTGG GCATCGTGCT GGAGGAGCTC GAGACCAATA TCATCCCCAT AAATCCTACG 
ATCATACGAG TCATGCGAGT GCTTCGAATA GCTCGAGTTC TGAAACTGCT CAAAATGGCG 
AAAGGTATAC GAGCATTATT GGACACGGTG ATGCAGGCGT TGCCACAGGT CGGAAACTTG 
GGACTATTGT TCTTCCTGTT GTTTTTCATT TTTGCGGCAC TGGGAGTGGA GTTGTTCGGC 
AGACTGGAAT GTTCCGAAGA GGTGCCATGC CAGGGATTGG GCGAGCATGC CCACTTCGCA 
AACTTCGGTA TGGCCTTTTT GACGTTGTTC AGAGTTGCGA CGGGAGATAA CTGGAATGGG 
ATCATGAAGG ACACACTTCG AGACGACTGT GACGATGCTG CCGATTGTGT TAAGAATTGT 
TGTGTTAGTA CTATAATAGC TCCCATATTT TTCGTTATAT TTGTACTGAT GGCTCAGTTT 
GTACTGGTCA ACGTGGTGGT TGCAGTTCTT ATGAAGCATC TTGAAGAAAG CCACAAACAG 
ATGGAAGATG AGTTAGATAT TGAAACAGAG CTGGAAAGGG AATTCGAACG TGAACAAGAA 
TTTGAGGAGG AACAAGCATT GTGTATGCAA CTTAATGACG ACCAAAAGCA ATTGCAAAAA 
CGGCCTCTCA CTAAAGTGTC TTCTTTGCCA TCTAACTTTA CTTATAGCAC TCCAGTTGCG 
GAGAAAAAAT TAAACATTCA TCGACGGCAA ACTATTCAAT ACATTAATCA GAATCTCAAT 
GTACAACATT CTTATACAAA CAGCAATTCG TATGACGAAA CTGCTGAAAA CGATATTCCG 
AATGAAGACG ACGAGTCTTT CAATTCTGTC AATGAAAGCT CTAGTACAAA GAATACCAAC 
AAGAAAATCC ATATTAACAA ATCTTTCTTT AGTAAAAATA ACGAAGATAA GCGAGCTTCA 
TTGGATGAAT TTAGCAAACC GCACAAAATT GTGGAGGAAT CGGGAAAACC CGATAAACCG 
AGCTCAACGA AGGGTTCAAA CAAATGGAAA GACAATCAGT CTGACCAACC CTCGAAAGAA 
GCGGAAAGCA ATACCAAACT GTCGACAATC ATTTCGTCCA ACAAATCGTT GACGAAAAGC 
AACAAACCCG ACTATCGTCA ATTAAGTCTT GATCAAGACG CCAAGCACGA CAACGTTACG 
CTTGCGCCCA ACACTAGTTC TTCTAATTTC TCAAACGCCT CCACTGCCCT CAACAACCTC 
GGTGGCAATA AATCCTCCTT TCTATCCGTA CCCAAACTGC AGCCAAAAAG TCGTTCAGGC 
AGCACAAAGC AACTGTTCAA ACAAGTGGCA CTGGACGAAG ATAATGAGAC CAACGAGAGT 
TCGCTCCTGC TGCCTTCGGT ATCGAATGCA GCCGGCTCTG GTGGCGATCA CTCGTACGAT 
CTTCTGAAGG TGCAGGACGA AAGTTACGAT GCTGTGCGAA AATCCGAATC CTGCGAGATT 
CTGAGGATCA TTTCCGAGCG ACGGAAGATC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL006849 ( XM_021850467.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021706159.1 Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X5, mRNA.

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CloneID	OAe126304
Clone ID Related Accession (Same CDS sequence)	XM_021850467.1
Accession Version	XM_021850467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACG CCTATTCGGA ATCACAATCC GATTTAGAAA ATGAGCAAAA CTTTGGAGCC 
AAACAGAAAC CACGCCCATC CGTCAACCGT CAATGCCAGG ACAGACGCAG CTCGTTGGTC 
AAACGTAGTG TCTCGGCAAA GAGCAATAGA CGGCCGAGCA TCAACAGCGT CTACAACGAG 
GGCCAGTTCA GCGAATCGTG CAGCATGCGA AAGAAACCTA ACAAATCCGT CGGGTCTGTT 
TCATCCACGA GCGGCACCAC GATGGACTCG TCCGGAAGCA GCTGCAGCGA TGGCGACACC 
TCGTCGTCCT ACGGGGAACC GAATCTTCCA TATCCGGGGT TTGCAGAGTA TTCGCTCAAG 
TATCTCAGCC AGGACACGAA ACCGAGAATA TGGTGCTTGC AGTTGATAAC CAATCCATGG 
TTCGAGCGAG TCTCAATGTT GGTGATTTTG CTGAATTGTG TTACATTGGG AATGTATCAA 
CCATGTGTTG ATGACGCCTG TGTGACGAAC CGCTGTAAAA TATTACAAAT ATTCGACGAT 
ATCATATTCG CTTTCTTTTC CCTCGAAATG ACAATCAAGA TAGTGGCGAT GGGTGCCTGG 
GGGAAGGGAA CCTATCTGGC GGATTCGTGG AACCGGCTGG ACTTCTTCAT TGTGCTGGCC 
GGAGCTTTGG AGTACTGCCT TCAGGTGGAA AACCTTAACC TAACCGCAAT CAGAACCATC 
CGTGTACTTA GACCACTGAG GGCAATCAAT CGGATACCGA GTATGCGAAT TCTTGTCATG 
CTATTGCTAG ACACGCTGCC CATGCTCGGT AATGTATTGC TGTTGTGCTT TTTCGTGTTC 
TTCATCTTCG GGATAGTGGG TGTACAGCTT TGGGAGGGCA TCTTGCGGCA GCGTTGCGTC 
ATAAAGCTTC CGGACAACGT GTCGGCTCCA GACACATTTA CACAACGTAA CCTTTACTAT 
AGAATTGTGA CCCAAAAGCA AAGGAAATCT GACATATCAT TTTACTACGA GTTTTCAAAG 
GAGCAGGACT ACATCTGCTC GAAGCCGGAC GACTCGGGAA TGCATCTGTG TAACAACCTT 
CCACCCTACC GGATAGGGCC ATTGGTTTGC AATGCTTCTG CCATGCCATT TTCGAACAAT 
GTACCCACAG CTCGGTCCTG CGTCAACTGG AATCAGTATT ACACCAACTG TACTCAGCTT 
GGGAACAATC CCTTCCAAGG GACGATATCT TTCGACAACA TCGGATTGGC CTGGGTCGCT 
ATATTTTTGG TCATATCACT CGAGGGATGG ACTGACATTA TGTATTATGT TCAAGACGCA 
CACAGTTTTT GGGATTGGAT ATATTTTGTG CTGCTGATTG TGATCGGATC ATTCTTTATG 
ATCAACCTTT GCCTAGTCGT AATTGCCACA CAGTTTTCGG AAACCAAGAA ACGGGAAATG 
GAGCGTATGC GCCAGGAGAG GGCTCGCTTT ACGTCGACCT CGACTTTGGC TTCCAGTACG 
AACAACTCCG AACCAACGAC TTGCTACGCA GAAATCGTCA AGTATATCGC ACATCTGTAC 
CGAAGGCTCA AGCGCCGCAT CATTAAGAAG TTACGACAGT ATAAATATCA ATACCAACAC 
CGGAAGGAAG GGTTGCTACC GCAAACTCCG GAAACCCTAA CATTGTCACC GAACAAGATC 
AAAGTCCACC ATCCAAAGTG TCCGAAAATG CACGCCCTAG CTGTGAGCAC TGTCCACAGG 
AATAATCAGG GTTCCAACAC GCCCTGTGCC ATCATCCCTA GGAACGCCCC GAGCGCTGTT 
GGCACCGAGA AGCAAATGTC CTCACCGGAC ATTTCGGAAA TGGTTTCCGT GGAGAACATC 
AAGAACAATG CTCTGAACAA TTGCACGAGC TATTCGAACT TGGATGACAA ACAGAAAATA 
GTCCTGCTGA AGATTCCAAA TAGTGAGAAC AATCAGTCCT CTTCAAACTC CCTCAGCCCG 
ACGTCCTCAG GTAGACGCAG ATCATCTGTT ATGTTTAATG AATATGTTAT ACATCATACG 
CCTCCAACTA TTCCAGCACC GACAACAGAT AAAAATGTGT ATTGCTTGGA AAAAATGACC 
CAAACCAGCG ATAGTGGAAT TTGGCAGGTC AGCCTACCAC AATCCATCCA AACCATGGCA 
ACGGTTTTCA ACGACTACTC AGACCTTTGC ATGACAGATG CTATGACCTG TCAAGAGCTA 
TTGGCCTTCT CGGTGGCCTT CTCCGCGGCA CTTCCAACGG GACACACAAC ATTGGAATCG 
TTCTACTCGA CGCTCACCAG AAATCGCACG AATCAAAGCA ATAATAAAAA TGCGTCAATT 
ACCAACATTC AAGGATACAG CGGCGATAAT GTCCAGCAGA AATCCCCGCC CAATGTGGCT 
AAAAATCTAT CGCAAAATGT ATCCAACATC AACAATATTA ACAACGAAGA GTTCGCCTGT 
TGCTACGATC TGTACCAAAA CGTGGCATTG GAAGAACCAC CGAAGAAACA CTCAAAGCCC 
TACAGATGCA TAACGGGAAC ATACAGATTT TTCAAAAGAG TACTACACTG TATCAGAGTG 
TACATCAAAA AGTTAGTGGA GCACAAGTAC TTTCAGCAAG GTATATTGCT GGCCATTTTG 
ATCAATACCC TTTCGATGGG CATCGAGTAC CACAATCAAC CGGAAGAGTT GACGGCAATT 
GTCGAGACGA GTAATATTGT GTTCTCTGGC ATCTTTGCTG TTGAGATGGT TCTTAAGGTG 
ATTGCGGAAG GACCGTTCGG ATATGTCGCT AATGGGTTTA ACGTGTTCGA TGGAGTAATT 
GTCATTCTTA GCGTTGTTGA ACTTGGTCAA GCATACCTGG GTGAAGGTCA AGGCAGTTCT 
GGCTTAAGTG TCCTAAGAAC GTTTCGCCTG CTACGTATAC TAAAGTTGGT ACGATTTATG 
CCAAACCTTC GGCGTCAACT GTTCGTGATG TTGAGAACTA TGGACAATGT GGCTATCTTC 
TTCTCCCTGC TGATACTGTT TATTTTCATA TTCAGCATAC TTGGCATGTA CTTGTTTGGA 
GGTAAGTTCT GTAAGTTTGT CGATGAAACT GGACAAGAGA GGGAATGCAC TTGTCCCGAA 
ATAGTCTCCA AGCATCCTCT GTGTGAATGT GATCGCAAAC ATTTCAACAA CATTCTCTGG 
GCCACGGTGA CAGTGTTTCA ACGAAACGAG CGAAGGGAAC GAGAGCAGCG GGAACTCGTG 
AAGGCCAAAC TCAATGCCGA GTCCTTGGCT GCTCAAGAGC AGGGGAGCGA GATCTACGAT 
GATCCCAAAA GCTTTTCAGA GTCAACCACT AGTGATAGCT ATAATGAGTC CAAGGGGAAA 
TGGTTCAGCG CTGAGGAGCT TCGGAAGATA AGGGACATAA AGTGCAACAT ACAGAAACAA 
AGGCTTTTGC AACCCGGGTA TGATGACATA AATCTGTCGA TTCCAAAGCT AAACTCGGAG 
AAAGATGCTC TCAATTCTAT AGATCTATAT TCCAACCGAA AGAAAAAATG CGAACCCCTT 
AAACTGTACA ACATCCAAGT GACGGATCCT CCGATCATAA CGACAACTGC CGCAACTCCT 
CAAGATTCGC CGAACGCAAC CCTCGAAGGA GGTGCCAGTT TCAAAGATTG GGAAAACCTG 
GACTTTGAAC AACTGGAGAA AAGTTCCAGC TCATCGCTAC TTCGTCCCCC ACCAATACTG 
GGATCTCTGA AAGCTTTGGA CGATCGAACG TACTTCGAAG GCATGCCGGT GCTAAGTGAA 
ATGCGCAGAA AGTCGGATAA GGGTACCGGT TCTCAAGGTG ACAGTAAATC AGCGGACCGG 
ATGCAGCAGT CGACGCAAGA CGGATCACGC AAGTCGGAAA CTACGCGGAA GCCTAGTCTG 
AAGAGTAGGA AGGATGCCCC ACGAGAAGGC GAAGTACTCA ACAACGGCAA ATGCGTTTCT 
CGTAGTGGAG GAATCACGCC ACTAGATGCC CGCAAAATCG ATCCCCTGTC GCAGAGGAGT 
AACAGGATAC AGAATGATAC CCAAAGAGAC AGACGAAATT CGTCCCGAAG AAGTTCCGTA 
AGGCGATCGT CGTCCATCAA GATCGACAGT GGACCCGGAA GCATCGCCAG CAATATAAGC 
GCTCCATCGT ACTCCAAGTA CATCTACAAC GACAAGAATA AGTATTACAT AGATAGGAAG 
AGTTCCCTTC GGTTGAGCGA CATAAAATCT CCGAACAACG CCCGGCGAAT GTCGTCCATA 
GAGCACACGT ATCACAAACA TTCGAGGGTA AGCGTGCACA ATCTGGAATT GAGGAAAATC 
CAAGAAGAAC TAACGAAAAA CAAGCTGAAC AACGCTTCGG TGACATCGGT CCAGGATGTA 
CTGCCCGATG CACCTGATAC CCGCATCAAG AACAAATCCA AGGGTCGCCT GAAGCAGTTC 
TTCCGCTACG TCACCCCGAA GAATTTTGTC GAGGAACGTG ATTTGTACAC TTTGTATATT 
TTCCCGGAGG ATAACAGATT CCGCCAGATT TGCAGTTGGT TCGTGAATCA GAAATGGTTC 
GACAATGTGA TATTGCTGTT CATAGCGCTG AATTGTATAA CGCTTGCCAT GGAGAGACCC 
AACATCCCGC CCACTTGCAC CGAGAGATAT TTCCTGTCCA CTGCGAACTA TGTTTTTACG 
GTTGTATTTG CAGTGGAGAT GTTCATCAAG GTTGTCGCTA CTGGAATGTT CTACGGTCGA 
GATGCGTATT TCACTTCCGG TTGGAACATT ATGGATGGTT CGCTTGTCAT AATTTCCATC 
GTCGATCTTT TGATGTCCTT GATCAGCGAG TCAAGTCCGA GAATTTTCGG CATATTGAGG 
GTCTTTCGAT TGTTAAGGTC GCTTAGACCT CTAAGGGTAA TCAACAGAGC ACCAGGATTG 
AAGTTGGTGG TTCAAACACT GCTTTCATCA TTGAGACCCA TCGGAAACAT AGTGTTGATA 
TGCTGCACAT TTTTCATCAT CTTTGGAATT CTTGGAGTGC AGCTGTTCAA GGGAACATTC 
TACTACTGCG AAGGGGAGAA CATCAAAGGT GTCAAGAACA AGACCGAGTG CTTGTCTATC 
GAAGGAAATG TTTGGGTAAA CCGGAAGTAC AACTTTGACG ATCTGGGTAA GGCGTTGATG 
TCTCTGTTTG TGCTGTCCTC TCGAGATGGC TGGGTGAACA TTATGTACAC TGGACTTGAT 
GCGGTCGGTG TTGACCAACA GCCAGTCGTA AACTACAACG AGTGGCGCTT GCTGTACTTT 
ATTGCGTTTA TCCTGCTGGT GGGATTTTTC GTGTTGAACA TGTTTGTGGG AGTTGTGGTC 
GAAAACTTTC ATCGCTGCCG CGAAGAGCAG GAAAAGGAAG AGAAAATACG TCGCGCTGCT 
AAGCGGGCGT TGCAGATGGA GAAGAAACGG CGACGAATGC ACGAACCGCC GTACTACACC 
AACTACTCGC CACTGCGGAT GTTCGTGCAC AACGTGGTAA CATCGAAGTA CTTCGACCTG 
GCAATAGCCG CCGTCATAGG CTTGAACGTG GTGACGATGG CCATGGAGTA CTACATGATG 
CCTCGAGCGT TGGAATACGC ACTAAAGATA TTCAACTATT TCTTCACCGC GGTTTTCATA 
CTGGAAGCCA TCATGAAACT GGTGGCGCTG GGCTTGAAGA TCTACATGAA AGACAAATGG 
AACCAACTGG ACGTGGCTAT TGTGATTCTG TCCATTGTGG GCATCGTGCT GGAGGAGCTC 
GAGACCAATA TCATCCCCAT AAATCCTACG ATCATACGAG TCATGCGAGT GCTTCGAATA 
GCTCGAGTTC TGAAACTGCT CAAAATGGCG AAAGGTATAC GAGCATTATT GGACACGGTG 
ATGCAGGCGT TGCCACAGGT CGGAAACTTG GGACTATTGT TCTTCCTGTT GTTTTTCATT 
TTTGCGGCAC TGGGAGTGGA GTTGTTCGGC AGACTGGAAT GTTCCGAAGA GGTGCCATGC 
CAGGGATTGG GCGAGCATGC CCACTTCGCA AACTTCGGTA TGGCCTTTTT GACGTTGTTC 
AGAGTTGCGA CGGGAGATAA CTGGAATGGG ATCATGAAGG ACACACTTCG AGACGACTGT 
GACGATGCTG CCGATTGTGT TAAGAATTGT TGTGTTAGTA CTATAATAGC TCCCATATTT 
TTCGTTATAT TTGTACTGAT GGCTCAGTTT GTACTGGTCA ACGTGGTGGT TGCAGTTCTT 
ATGAAGCATC TTGAAGAAAG CCACAAACAG ATGGAAGATG AGTTAGATAT TGAAACAGAG 
CTGGAAAGGG AATTCGAACG TGAACAAGAA TTTGAGGAGG AACAAGCATT GTGTATGCAA 
CTTAATGACG ACCAAAAGCA ATTGCAAAAA CGGCCTCTCA CTAAAGTGTC TTCTTTGCCA 
TCTAACTTTA CTTATAGCAC TCCAGTTGCG GAGAAAAAAT TAAACATTCA TCGACGGCAA 
ACTATTCAAT ACATTAATCA GAATCTCAAT GTACAACATT CTTATACAAA CAGCAATTCG 
TATGACGAAA CTGCTGAAAA CGATATTCCG AATGAAGACG ACGAGTCTTT CAATTCTGTC 
AATGAAAGCT CTAGTACAAA GAATACCAAC AAGAAAATCC ATATTAACAA ATCTTTCTTT 
AGTAAAAATA ACGAAGATAA GCGAGCTTCA TTGGATGAAT TTAGCAAACC GCACAAAATT 
GTGGAGGAAT CGGGAAAACC CGATAAACCG AGCTCAACGA AGGGTTCAAA CAAATGGAAA 
GACAATCAGT CTGACCAACC CTCGAAAGAA GCGGAAAGCA ATACCAAACT GTCGACAATC 
ATTTCGTCCA ACAAATCGTT GACGAAAAGC AACAAACCCG ACTATCGTCA ATTAAGTCTT 
GATCAAGACG CCAAGCACGA CAACGTTACG CTTGCGCCCA ACACTAGTTC TTCTAATTTC 
TCAAACGCCT CCACTGCCCT CAACAACCTC GGTGGCAATA AATCCTCCTT TCTATCCGTA 
CCCAAACTGC AGCCAAAAAG TCGTTCAGGC AGCACAAAGC AACTGTTCAA ACAAGTGGCA 
CTGGACGAAG ATAATGAGAC CAACGAGAGT TCGCTCCTGC TGCCTTCGGT ATCGAATGCA 
GCCGGCTCTG GTGGCGATCA CTCGTACGAT CTTCTGAAGG TGCAGGACGA AAGTTACGAT 
GCTGTGCGAA AATCCGAATC CTGCGAGATT CTGAGGATCA TTTCCGAGCG ACGGAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021706159.1
CDS	167..7429
Translation	MLNAYSESQSDLENEQNFGAKQKPRPSVNRQCQDRRSSLVKRSVSAKSNRRPSINSVYNEGQFSESCSMRKKPNKSVGSVSSTSGTTMDSSGSSCSDGDTSSSYGEPNLPYPGFAEYSLKYLSQDTKPRIWCLQLITNPWFERVSMLVILLNCVTLGMYQPCVDDACVTNRCKILQIFDDIIFAFFSLEMTIKIVAMGAWGKGTYLADSWNRLDFFIVLAGALEYCLQVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWEGILRQRCVIKLPDNVSAPDTFTQRNLYYRIVTQKQRKSDISFYYEFSKEQDYICSKPDDSGMHLCNNLPPYRIGPLVCNASAMPFSNNVPTARSCVNWNQYYTNCTQLGNNPFQGTISFDNIGLAWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERARFTSTSTLASSTNNSEPTTCYAEIVKYIAHLYRRLKRRIIKKLRQYKYQYQHRKEGLLPQTPETLTLSPNKIKVHHPKCPKMHALAVSTVHRNNQGSNTPCAIIPRNAPSAVGTEKQMSSPDISEMVSVENIKNNALNNCTSYSNLDDKQKIVLLKIPNSENNQSSSNSLSPTSSGRRRSSVMFNEYVIHHTPPTIPAPTTDKNVYCLEKMTQTSDSGIWQVSLPQSIQTMATVFNDYSDLCMTDAMTCQELLAFSVAFSAALPTGHTTLESFYSTLTRNRTNQSNNKNASITNIQGYSGDNVQQKSPPNVAKNLSQNVSNINNINNEEFACCYDLYQNVALEEPPKKHSKPYRCITGTYRFFKRVLHCIRVYIKKLVEHKYFQQGILLAILINTLSMGIEYHNQPEELTAIVETSNIVFSGIFAVEMVLKVIAEGPFGYVANGFNVFDGVIVILSVVELGQAYLGEGQGSSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAIFFSLLILFIFIFSILGMYLFGGKFCKFVDETGQERECTCPEIVSKHPLCECDRKHFNNILWATVTVFQRNERREREQRELVKAKLNAESLAAQEQGSEIYDDPKSFSESTTSDSYNESKGKWFSAEELRKIRDIKCNIQKQRLLQPGYDDINLSIPKLNSEKDALNSIDLYSNRKKKCEPLKLYNIQVTDPPIITTTAATPQDSPNATLEGGASFKDWENLDFEQLEKSSSSSLLRPPPILGSLKALDDRTYFEGMPVLSEMRRKSDKGTGSQGDSKSADRMQQSTQDGSRKSETTRKPSLKSRKDAPREGEVLNNGKCVSRSGGITPLDARKIDPLSQRSNRIQNDTQRDRRNSSRRSSVRRSSSIKIDSGPGSIASNISAPSYSKYIYNDKNKYYIDRKSSLRLSDIKSPNNARRMSSIEHTYHKHSRVSVHNLELRKIQEELTKNKLNNASVTSVQDVLPDAPDTRIKNKSKGRLKQFFRYVTPKNFVEERDLYTLYIFPEDNRFRQICSWFVNQKWFDNVILLFIALNCITLAMERPNIPPTCTERYFLSTANYVFTVVFAVEMFIKVVATGMFYGRDAYFTSGWNIMDGSLVIISIVDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVKNKTECLSIEGNVWVNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPVVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPLRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYMMPRALEYALKIFNYFFTAVFILEAIMKLVALGLKIYMKDKWNQLDVAIVILSIVGIVLEELETNIIPINPTIIRVMRVLRIARVLKLLKMAKGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSEEVPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDDCDDAADCVKNCCVSTIIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHKQMEDELDIETELEREFEREQEFEEEQALCMQLNDDQKQLQKRPLTKVSSLPSNFTYSTPVAEKKLNIHRRQTIQYINQNLNVQHSYTNSNSYDETAENDIPNEDDESFNSVNESSSTKNTNKKIHINKSFFSKNNEDKRASLDEFSKPHKIVEESGKPDKPSSTKGSNKWKDNQSDQPSKEAESNTKLSTIISSNKSLTKSNKPDYRQLSLDQDAKHDNVTLAPNTSSSNFSNASTALNNLGGNKSSFLSVPKLQPKSRSGSTKQLFKQVALDEDNETNESSLLLPSVSNAAGSGGDHSYDLLKVQDESYDAVRKSESCEILRIISERRKI

Target ORF information:

RefSeq Version	XM_021850467.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti voltage-dependent T-type calcium channel subunit alpha-1G (LOC5568434), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021850467.1

ORF Insert Sequence:

ATGTTGAACG CCTATTCGGA ATCACAATCC GATTTAGAAA ATGAGCAAAA CTTTGGAGCC 
AAACAGAAAC CACGCCCATC CGTCAACCGT CAATGCCAGG ACAGACGCAG CTCGTTGGTC 
AAACGTAGTG TCTCGGCAAA GAGCAATAGA CGGCCGAGCA TCAACAGCGT CTACAACGAG 
GGCCAGTTCA GCGAATCGTG CAGCATGCGA AAGAAACCTA ACAAATCCGT CGGGTCTGTT 
TCATCCACGA GCGGCACCAC GATGGACTCG TCCGGAAGCA GCTGCAGCGA TGGCGACACC 
TCGTCGTCCT ACGGGGAACC GAATCTTCCA TATCCGGGGT TTGCAGAGTA TTCGCTCAAG 
TATCTCAGCC AGGACACGAA ACCGAGAATA TGGTGCTTGC AGTTGATAAC CAATCCATGG 
TTCGAGCGAG TCTCAATGTT GGTGATTTTG CTGAATTGTG TTACATTGGG AATGTATCAA 
CCATGTGTTG ATGACGCCTG TGTGACGAAC CGCTGTAAAA TATTACAAAT ATTCGACGAT 
ATCATATTCG CTTTCTTTTC CCTCGAAATG ACAATCAAGA TAGTGGCGAT GGGTGCCTGG 
GGGAAGGGAA CCTATCTGGC GGATTCGTGG AACCGGCTGG ACTTCTTCAT TGTGCTGGCC 
GGAGCTTTGG AGTACTGCCT TCAGGTGGAA AACCTTAACC TAACCGCAAT CAGAACCATC 
CGTGTACTTA GACCACTGAG GGCAATCAAT CGGATACCGA GTATGCGAAT TCTTGTCATG 
CTATTGCTAG ACACGCTGCC CATGCTCGGT AATGTATTGC TGTTGTGCTT TTTCGTGTTC 
TTCATCTTCG GGATAGTGGG TGTACAGCTT TGGGAGGGCA TCTTGCGGCA GCGTTGCGTC 
ATAAAGCTTC CGGACAACGT GTCGGCTCCA GACACATTTA CACAACGTAA CCTTTACTAT 
AGAATTGTGA CCCAAAAGCA AAGGAAATCT GACATATCAT TTTACTACGA GTTTTCAAAG 
GAGCAGGACT ACATCTGCTC GAAGCCGGAC GACTCGGGAA TGCATCTGTG TAACAACCTT 
CCACCCTACC GGATAGGGCC ATTGGTTTGC AATGCTTCTG CCATGCCATT TTCGAACAAT 
GTACCCACAG CTCGGTCCTG CGTCAACTGG AATCAGTATT ACACCAACTG TACTCAGCTT 
GGGAACAATC CCTTCCAAGG GACGATATCT TTCGACAACA TCGGATTGGC CTGGGTCGCT 
ATATTTTTGG TCATATCACT CGAGGGATGG ACTGACATTA TGTATTATGT TCAAGACGCA 
CACAGTTTTT GGGATTGGAT ATATTTTGTG CTGCTGATTG TGATCGGATC ATTCTTTATG 
ATCAACCTTT GCCTAGTCGT AATTGCCACA CAGTTTTCGG AAACCAAGAA ACGGGAAATG 
GAGCGTATGC GCCAGGAGAG GGCTCGCTTT ACGTCGACCT CGACTTTGGC TTCCAGTACG 
AACAACTCCG AACCAACGAC TTGCTACGCA GAAATCGTCA AGTATATCGC ACATCTGTAC 
CGAAGGCTCA AGCGCCGCAT CATTAAGAAG TTACGACAGT ATAAATATCA ATACCAACAC 
CGGAAGGAAG GGTTGCTACC GCAAACTCCG GAAACCCTAA CATTGTCACC GAACAAGATC 
AAAGTCCACC ATCCAAAGTG TCCGAAAATG CACGCCCTAG CTGTGAGCAC TGTCCACAGG 
AATAATCAGG GTTCCAACAC GCCCTGTGCC ATCATCCCTA GGAACGCCCC GAGCGCTGTT 
GGCACCGAGA AGCAAATGTC CTCACCGGAC ATTTCGGAAA TGGTTTCCGT GGAGAACATC 
AAGAACAATG CTCTGAACAA TTGCACGAGC TATTCGAACT TGGATGACAA ACAGAAAATA 
GTCCTGCTGA AGATTCCAAA TAGTGAGAAC AATCAGTCCT CTTCAAACTC CCTCAGCCCG 
ACGTCCTCAG GTAGACGCAG ATCATCTGTT ATGTTTAATG AATATGTTAT ACATCATACG 
CCTCCAACTA TTCCAGCACC GACAACAGAT AAAAATGTGT ATTGCTTGGA AAAAATGACC 
CAAACCAGCG ATAGTGGAAT TTGGCAGGTC AGCCTACCAC AATCCATCCA AACCATGGCA 
ACGGTTTTCA ACGACTACTC AGACCTTTGC ATGACAGATG CTATGACCTG TCAAGAGCTA 
TTGGCCTTCT CGGTGGCCTT CTCCGCGGCA CTTCCAACGG GACACACAAC ATTGGAATCG 
TTCTACTCGA CGCTCACCAG AAATCGCACG AATCAAAGCA ATAATAAAAA TGCGTCAATT 
ACCAACATTC AAGGATACAG CGGCGATAAT GTCCAGCAGA AATCCCCGCC CAATGTGGCT 
AAAAATCTAT CGCAAAATGT ATCCAACATC AACAATATTA ACAACGAAGA GTTCGCCTGT 
TGCTACGATC TGTACCAAAA CGTGGCATTG GAAGAACCAC CGAAGAAACA CTCAAAGCCC 
TACAGATGCA TAACGGGAAC ATACAGATTT TTCAAAAGAG TACTACACTG TATCAGAGTG 
TACATCAAAA AGTTAGTGGA GCACAAGTAC TTTCAGCAAG GTATATTGCT GGCCATTTTG 
ATCAATACCC TTTCGATGGG CATCGAGTAC CACAATCAAC CGGAAGAGTT GACGGCAATT 
GTCGAGACGA GTAATATTGT GTTCTCTGGC ATCTTTGCTG TTGAGATGGT TCTTAAGGTG 
ATTGCGGAAG GACCGTTCGG ATATGTCGCT AATGGGTTTA ACGTGTTCGA TGGAGTAATT 
GTCATTCTTA GCGTTGTTGA ACTTGGTCAA GCATACCTGG GTGAAGGTCA AGGCAGTTCT 
GGCTTAAGTG TCCTAAGAAC GTTTCGCCTG CTACGTATAC TAAAGTTGGT ACGATTTATG 
CCAAACCTTC GGCGTCAACT GTTCGTGATG TTGAGAACTA TGGACAATGT GGCTATCTTC 
TTCTCCCTGC TGATACTGTT TATTTTCATA TTCAGCATAC TTGGCATGTA CTTGTTTGGA 
GGTAAGTTCT GTAAGTTTGT CGATGAAACT GGACAAGAGA GGGAATGCAC TTGTCCCGAA 
ATAGTCTCCA AGCATCCTCT GTGTGAATGT GATCGCAAAC ATTTCAACAA CATTCTCTGG 
GCCACGGTGA CAGTGTTTCA ACGAAACGAG CGAAGGGAAC GAGAGCAGCG GGAACTCGTG 
AAGGCCAAAC TCAATGCCGA GTCCTTGGCT GCTCAAGAGC AGGGGAGCGA GATCTACGAT 
GATCCCAAAA GCTTTTCAGA GTCAACCACT AGTGATAGCT ATAATGAGTC CAAGGGGAAA 
TGGTTCAGCG CTGAGGAGCT TCGGAAGATA AGGGACATAA AGTGCAACAT ACAGAAACAA 
AGGCTTTTGC AACCCGGGTA TGATGACATA AATCTGTCGA TTCCAAAGCT AAACTCGGAG 
AAAGATGCTC TCAATTCTAT AGATCTATAT TCCAACCGAA AGAAAAAATG CGAACCCCTT 
AAACTGTACA ACATCCAAGT GACGGATCCT CCGATCATAA CGACAACTGC CGCAACTCCT 
CAAGATTCGC CGAACGCAAC CCTCGAAGGA GGTGCCAGTT TCAAAGATTG GGAAAACCTG 
GACTTTGAAC AACTGGAGAA AAGTTCCAGC TCATCGCTAC TTCGTCCCCC ACCAATACTG 
GGATCTCTGA AAGCTTTGGA CGATCGAACG TACTTCGAAG GCATGCCGGT GCTAAGTGAA 
ATGCGCAGAA AGTCGGATAA GGGTACCGGT TCTCAAGGTG ACAGTAAATC AGCGGACCGG 
ATGCAGCAGT CGACGCAAGA CGGATCACGC AAGTCGGAAA CTACGCGGAA GCCTAGTCTG 
AAGAGTAGGA AGGATGCCCC ACGAGAAGGC GAAGTACTCA ACAACGGCAA ATGCGTTTCT 
CGTAGTGGAG GAATCACGCC ACTAGATGCC CGCAAAATCG ATCCCCTGTC GCAGAGGAGT 
AACAGGATAC AGAATGATAC CCAAAGAGAC AGACGAAATT CGTCCCGAAG AAGTTCCGTA 
AGGCGATCGT CGTCCATCAA GATCGACAGT GGACCCGGAA GCATCGCCAG CAATATAAGC 
GCTCCATCGT ACTCCAAGTA CATCTACAAC GACAAGAATA AGTATTACAT AGATAGGAAG 
AGTTCCCTTC GGTTGAGCGA CATAAAATCT CCGAACAACG CCCGGCGAAT GTCGTCCATA 
GAGCACACGT ATCACAAACA TTCGAGGGTA AGCGTGCACA ATCTGGAATT GAGGAAAATC 
CAAGAAGAAC TAACGAAAAA CAAGCTGAAC AACGCTTCGG TGACATCGGT CCAGGATGTA 
CTGCCCGATG CACCTGATAC CCGCATCAAG AACAAATCCA AGGGTCGCCT GAAGCAGTTC 
TTCCGCTACG TCACCCCGAA GAATTTTGTC GAGGAACGTG ATTTGTACAC TTTGTATATT 
TTCCCGGAGG ATAACAGATT CCGCCAGATT TGCAGTTGGT TCGTGAATCA GAAATGGTTC 
GACAATGTGA TATTGCTGTT CATAGCGCTG AATTGTATAA CGCTTGCCAT GGAGAGACCC 
AACATCCCGC CCACTTGCAC CGAGAGATAT TTCCTGTCCA CTGCGAACTA TGTTTTTACG 
GTTGTATTTG CAGTGGAGAT GTTCATCAAG GTTGTCGCTA CTGGAATGTT CTACGGTCGA 
GATGCGTATT TCACTTCCGG TTGGAACATT ATGGATGGTT CGCTTGTCAT AATTTCCATC 
GTCGATCTTT TGATGTCCTT GATCAGCGAG TCAAGTCCGA GAATTTTCGG CATATTGAGG 
GTCTTTCGAT TGTTAAGGTC GCTTAGACCT CTAAGGGTAA TCAACAGAGC ACCAGGATTG 
AAGTTGGTGG TTCAAACACT GCTTTCATCA TTGAGACCCA TCGGAAACAT AGTGTTGATA 
TGCTGCACAT TTTTCATCAT CTTTGGAATT CTTGGAGTGC AGCTGTTCAA GGGAACATTC 
TACTACTGCG AAGGGGAGAA CATCAAAGGT GTCAAGAACA AGACCGAGTG CTTGTCTATC 
GAAGGAAATG TTTGGGTAAA CCGGAAGTAC AACTTTGACG ATCTGGGTAA GGCGTTGATG 
TCTCTGTTTG TGCTGTCCTC TCGAGATGGC TGGGTGAACA TTATGTACAC TGGACTTGAT 
GCGGTCGGTG TTGACCAACA GCCAGTCGTA AACTACAACG AGTGGCGCTT GCTGTACTTT 
ATTGCGTTTA TCCTGCTGGT GGGATTTTTC GTGTTGAACA TGTTTGTGGG AGTTGTGGTC 
GAAAACTTTC ATCGCTGCCG CGAAGAGCAG GAAAAGGAAG AGAAAATACG TCGCGCTGCT 
AAGCGGGCGT TGCAGATGGA GAAGAAACGG CGACGAATGC ACGAACCGCC GTACTACACC 
AACTACTCGC CACTGCGGAT GTTCGTGCAC AACGTGGTAA CATCGAAGTA CTTCGACCTG 
GCAATAGCCG CCGTCATAGG CTTGAACGTG GTGACGATGG CCATGGAGTA CTACATGATG 
CCTCGAGCGT TGGAATACGC ACTAAAGATA TTCAACTATT TCTTCACCGC GGTTTTCATA 
CTGGAAGCCA TCATGAAACT GGTGGCGCTG GGCTTGAAGA TCTACATGAA AGACAAATGG 
AACCAACTGG ACGTGGCTAT TGTGATTCTG TCCATTGTGG GCATCGTGCT GGAGGAGCTC 
GAGACCAATA TCATCCCCAT AAATCCTACG ATCATACGAG TCATGCGAGT GCTTCGAATA 
GCTCGAGTTC TGAAACTGCT CAAAATGGCG AAAGGTATAC GAGCATTATT GGACACGGTG 
ATGCAGGCGT TGCCACAGGT CGGAAACTTG GGACTATTGT TCTTCCTGTT GTTTTTCATT 
TTTGCGGCAC TGGGAGTGGA GTTGTTCGGC AGACTGGAAT GTTCCGAAGA GGTGCCATGC 
CAGGGATTGG GCGAGCATGC CCACTTCGCA AACTTCGGTA TGGCCTTTTT GACGTTGTTC 
AGAGTTGCGA CGGGAGATAA CTGGAATGGG ATCATGAAGG ACACACTTCG AGACGACTGT 
GACGATGCTG CCGATTGTGT TAAGAATTGT TGTGTTAGTA CTATAATAGC TCCCATATTT 
TTCGTTATAT TTGTACTGAT GGCTCAGTTT GTACTGGTCA ACGTGGTGGT TGCAGTTCTT 
ATGAAGCATC TTGAAGAAAG CCACAAACAG ATGGAAGATG AGTTAGATAT TGAAACAGAG 
CTGGAAAGGG AATTCGAACG TGAACAAGAA TTTGAGGAGG AACAAGCATT GTGTATGCAA 
CTTAATGACG ACCAAAAGCA ATTGCAAAAA CGGCCTCTCA CTAAAGTGTC TTCTTTGCCA 
TCTAACTTTA CTTATAGCAC TCCAGTTGCG GAGAAAAAAT TAAACATTCA TCGACGGCAA 
ACTATTCAAT ACATTAATCA GAATCTCAAT GTACAACATT CTTATACAAA CAGCAATTCG 
TATGACGAAA CTGCTGAAAA CGATATTCCG AATGAAGACG ACGAGTCTTT CAATTCTGTC 
AATGAAAGCT CTAGTACAAA GAATACCAAC AAGAAAATCC ATATTAACAA ATCTTTCTTT 
AGTAAAAATA ACGAAGATAA GCGAGCTTCA TTGGATGAAT TTAGCAAACC GCACAAAATT 
GTGGAGGAAT CGGGAAAACC CGATAAACCG AGCTCAACGA AGGGTTCAAA CAAATGGAAA 
GACAATCAGT CTGACCAACC CTCGAAAGAA GCGGAAAGCA ATACCAAACT GTCGACAATC 
ATTTCGTCCA ACAAATCGTT GACGAAAAGC AACAAACCCG ACTATCGTCA ATTAAGTCTT 
GATCAAGACG CCAAGCACGA CAACGTTACG CTTGCGCCCA ACACTAGTTC TTCTAATTTC 
TCAAACGCCT CCACTGCCCT CAACAACCTC GGTGGCAATA AATCCTCCTT TCTATCCGTA 
CCCAAACTGC AGCCAAAAAG TCGTTCAGGC AGCACAAAGC AACTGTTCAA ACAAGTGGCA 
CTGGACGAAG ATAATGAGAC CAACGAGAGT TCGCTCCTGC TGCCTTCGGT ATCGAATGCA 
GCCGGCTCTG GTGGCGATCA CTCGTACGAT CTTCTGAAGG TGCAGGACGA AAGTTACGAT 
GCTGTGCGAA AATCCGAATC CTGCGAGATT CTGAGGATCA TTTCCGAGCG ACGGAAGATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL006849 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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