LOC5568238 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5568238
Entrez Gene ID	5568238
Full Name	protein smoothened
Synonyms	AAEL006669,GPRSMO
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001657877.2	XP_001657927.2	AAEL006669-PA
XM_001657877.3	XP_001657927.2	protein smoothened

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5568238 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5568238 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe14487	XM_001657877.3 Latest version!	Aedes aegypti protein smoothened (LOC5568238), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OAe14487	XM_001657877.2	Aedes aegypti AAEL006669-RA (GPRSMO), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5568238 ( XM_001657877.2 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001657927.2 Aedes aegypti AAEL006669-RA (GPRSMO), partial mRNA.

Price & Availability↑

CloneID	OAe14487
Clone ID Related Accession (Same CDS sequence)	XM_001657877.2 , XM_001657877.3
Accession Version	XM_001657877.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL006669-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810864). On Mar 5, 2015 this sequence version replaced XM_001657877.1. COMPLETENESS: incomplete on both ends.

ATGGGAGCCC AATCACTGTT GTCGTCGGTG ACCCTGCTGG TTCTGTTTGG AATCACTGCC 
AACGCGAAAG AATTCACCCC GTCCACGAAT CTCATCACAC CTGTGGACTC GCCCCGACTC 
GAGGCCATCA ATGGAACCCG AAATTTCCGC ATGCACGGTA AGAAAGGCAA GGATGACAAA 
ACCTGGTTCG ATGGCCGCGA GATGCGTTAC ATCTACTGCG TGCGACCGGC CAAGTGCGAA 
CCGATTCGGC ACAAGTCCTG TCTGGGCTCC TCACTGCCGT ACTCCTCCAT CAGTCTGGAT 
CTGACCGATT CCTTCAGCCA GGAGCAAACC CACGATAAAC TGTACCAGTA CAATGCGTTG 
CGTCACGTCC CGAAGTGCTG GGCCGTTATT CAGCCATTTT TGTGCGCCGT GTTCACACCC 
AAGTGCGAAA AGATCAACGG TCGCGACATG GTCTACCTTC CGTCGCTGGA GATGTGCAAG 
ATCACGCTGG AACCGTGTCG GATACTGTAC AACACCAGCT ACTTTCCGGA GTTCCTGAAG 
TGTAACGAAA CGCTGTATCC CTCGAAGTGC AACAACGACG TACGGGAGGA GATGAAGTTC 
AACGCTACCG GGCAGTGTCT CAAACCGCTG GTCGCGGCGG AGTCGCCGGC GAATTACTAT 
AAAGACATCG ACGGATGCGG TCTGCAGTGC AAGGATCCGC TCTACACCGA TCACGAGCAT 
CGACAGATCC ACAAGCTGAT TGCGTGGGGT GCCGGCGTGT GCTTGACGTT CAACTTGTTC 
ACGATCGCCA CGTTTGTGAT CGATTGGCAC AATGCGAACA AGTACCCGGC GTTGGTGATT 
TTCTACATTA ACTTTTGCTT CATGGTTTCG TGCATGGGTT GGCTAGCTCA ATTCACGCCC 
GGAGGTCGAG AGGATATCGT CTGCCGAAAG GATGGAACCT TGCGCATTTC GGAACCCAGT 
GCCGGTGAGA ATCTGTCATG TATTGTCGTG TTTATTCTGG TGTACTATTT CTTGATTGCG 
GCCATGGTTT GGTTCGTCAT TTTTACGTAC GTATGGCATA TGAGTTTCAA AGCTATTGGT 
AAAATTCAGG ATCGAATAGA TAAGAAAGGA TCTTATTTCC ATTTAATTGC TTGGTCACTG 
CCTCTAGTGT TGACGATAAC GATCATGGCT CTGAGCGAGA TCGATGGTAA CAGCACGGTG 
GGAATATGCT TCGTGGGGTA TTTGAATCAC CCGATCAGGG CGGGATTCCT TTTGGGTCCG 
GTGGTATGTG TACTTTTCAT CGGAGGTTAT TTTCTGGGAC GAGGATTGCT AACACTGATA 
AAGCTAAAGA TTTCCAGTAA AGAAATAATT TCGGCTCGTG CAAGTAAGAA AATCAGACAG 
ACGATCGTAC GAATGGGAAT TTGTACGATG TTCACGTTCG TCTTCATTGT TTCGACTATC 
TTCTGTCATA TCCACGAGTT CAAAAACACT GAAAGTTGGA CGATTGCATT GAAGAACTAC 
ATAATTTGTC AGATATCGTC GACTTATACG GATGTGACGA GCACTTGTAA GCTTCATAGC 
CGCCCAAGTG TGGCCGTGCT TCAGATTCAT CTTATTTGCT TGTTTGCGTC CGGAATCGTG 
ATGTCCTCGT GGGTCTGGAC CAACTCCACT CTGGAAACGT GGGGTCGGTA TTTTCGGAGG 
CGCTTCCGAC CAGACGTCGA GGAACCCGTG AAGCTGCAGA AGCATAAGGT TATTGCTCAG 
GCGTTTGCGA AGCGGAAGGA GTTTCAGAAC CAGGGACGAC TCTCGATTTC CTTCCACAAC 
TCCCACACCG ATCCCGTTGG CTTGAAGTTC GATATCAATT CGGCCATCGG GAGTCACGAT 
TTCAGCTCGA CATGGGCGAA TAACCTGCCC AGGTTTGTTA ACAGGCGTTA TGCATTGACG 
GGAGCTGCTA CGAGTTCGAG TCATGATCCG AGAAAGAATT CTGTAGACTC GGAGATAAGC 
TTCAGCGTGC GTCATGTTTC GGTGGAGTCC AGAAGGAACT CGATAGATTC TCAGGTTTCG 
GTAAAGATAG CCGAGATGAA GACCAAGGTC GCAAGCAGGA GTCGCAGTGG AGTTAGTAGC 
AGCAAGCATC ATCATAAGAA TCGCCAACAG AGGCGGCGAG ACTTCACTGC TTCCGGCAGA 
AGATACAGTC GAAAAGAAAG TAGCACTTCG GTAGAATCTC AAATAATAAC GGCAATCCAG 
AAGGGCAGAC ATTCCTCTAG TGGAAACAGT CAATTTTTCG GGGCTAACAA TATGCAACGG 
AGAACTGGTA TCGGTGGTAT GGATGCTGAC CAACTCAACG ATTTTATTTC CAACGGAAAA 
CTATTGCTTC CATTCCTGCA TCAGCCCGGC TTGACGACTT CAGAAGACGA TAACGTCTCA 
AATGCATCAT TCAAGGTTCA GGATTCAAAG TTCGATGTAA TTCTGAAGCA TATCGGAAGT 
GCGGGGACAT CGAGCCGGGT TGATTCACAT AACGCAGGCT GCCAAATCGA AGAATACCAT 
TCCAGTGACG ACGATGAAAA GCTAAGCTTG AAGCAAGCCA GCAGAGACGA ATTCTCCACG 
ATGAAAGATG GCATGAAGAG CAGTAAGGAA GGTGGACGAG GCAGTAAAAA CAGTTCCAAA 
AGTGTTCGCA GTAAGAAATC ATCATCCCGC CAGGGTACCA TTAGAAAGTC CCAAAAGCAT 
TCACGAGCCA GTCAGAAAAG CCAGCGGAAT GAAAAGAAGG AAAAAGCTCG GGAACGAGAA 
CGCGAAAAGG AACGAGAGAA AGAGCGGGAA AAAGAGCTGC AACTCATCGA GCGAAACAAC 
ATTACCGATT TCAACGATTC CTCCTTCACT TCATACTGCT CGGAACTGTC CCCTCTCAAT 
GTGAACAGTT CCTACTCGGG AATCTCACTC GCCAAAACGA ACTCTCGTAA TTCCAAACGT 
AGCTGCGATG TTGGAATTCA GGCGAATGCT TACGAAATCG CCACCCAAAC CATGTCTTCG 
TTCGAGTTCA ACGAAAAAGC GCTGAAAAAT GAAGAGAACG AAGACATCTA CACAGAAAAC 
CACCAGCTGC TAGGGCCCAA ATTGGTACCC CAAGTAGTGC AAGCCCCACG CAAGCGTGAC 
CTCGCGGACG TAAGCTTAAC CGAGGCGGAG AAACTCAAAC AGTTGCTACT TCCCTCAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001657927.2
CDS	1..3183
Translation	MGAQSLLSSVTLLVLFGITANAKEFTPSTNLITPVDSPRLEAINGTRNFRMHGKKGKDDKTWFDGREMRYIYCVRPAKCEPIRHKSCLGSSLPYSSISLDLTDSFSQEQTHDKLYQYNALRHVPKCWAVIQPFLCAVFTPKCEKINGRDMVYLPSLEMCKITLEPCRILYNTSYFPEFLKCNETLYPSKCNNDVREEMKFNATGQCLKPLVAAESPANYYKDIDGCGLQCKDPLYTDHEHRQIHKLIAWGAGVCLTFNLFTIATFVIDWHNANKYPALVIFYINFCFMVSCMGWLAQFTPGGREDIVCRKDGTLRISEPSAGENLSCIVVFILVYYFLIAAMVWFVIFTYVWHMSFKAIGKIQDRIDKKGSYFHLIAWSLPLVLTITIMALSEIDGNSTVGICFVGYLNHPIRAGFLLGPVVCVLFIGGYFLGRGLLTLIKLKISSKEIISARASKKIRQTIVRMGICTMFTFVFIVSTIFCHIHEFKNTESWTIALKNYIICQISSTYTDVTSTCKLHSRPSVAVLQIHLICLFASGIVMSSWVWTNSTLETWGRYFRRRFRPDVEEPVKLQKHKVIAQAFAKRKEFQNQGRLSISFHNSHTDPVGLKFDINSAIGSHDFSSTWANNLPRFVNRRYALTGAATSSSHDPRKNSVDSEISFSVRHVSVESRRNSIDSQVSVKIAEMKTKVASRSRSGVSSSKHHHKNRQQRRRDFTASGRRYSRKESSTSVESQIITAIQKGRHSSSGNSQFFGANNMQRRTGIGGMDADQLNDFISNGKLLLPFLHQPGLTTSEDDNVSNASFKVQDSKFDVILKHIGSAGTSSRVDSHNAGCQIEEYHSSDDDEKLSLKQASRDEFSTMKDGMKSSKEGGRGSKNSSKSVRSKKSSSRQGTIRKSQKHSRASQKSQRNEKKEKAREREREKEREKEREKELQLIERNNITDFNDSSFTSYCSELSPLNVNSSYSGISLAKTNSRNSKRSCDVGIQANAYEIATQTMSSFEFNEKALKNEENEDIYTENHQLLGPKLVPQVVQAPRKRDLADVSLTEAEKLKQLLLPSK

Target ORF information:

RefSeq Version	XM_001657877.2
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL006669-RA (GPRSMO), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001657877.2

ORF Insert Sequence:

ATGGGAGCCC AATCACTGTT GTCGTCGGTG ACCCTGCTGG TTCTGTTTGG AATCACTGCC 
AACGCGAAAG AATTCACCCC GTCCACGAAT CTCATCACAC CTGTGGACTC GCCCCGACTC 
GAGGCCATCA ATGGAACCCG AAATTTCCGC ATGCACGGTA AGAAAGGCAA GGATGACAAA 
ACCTGGTTCG ATGGCCGCGA GATGCGTTAC ATCTACTGCG TGCGACCGGC CAAGTGCGAA 
CCGATTCGGC ACAAGTCCTG TCTGGGCTCC TCACTGCCGT ACTCCTCCAT CAGTCTGGAT 
CTGACCGATT CCTTCAGCCA GGAGCAAACC CACGATAAAC TGTACCAGTA CAATGCGTTG 
CGTCACGTCC CGAAGTGCTG GGCCGTTATT CAGCCATTTT TGTGCGCCGT GTTCACACCC 
AAGTGCGAAA AGATCAACGG TCGCGACATG GTCTACCTTC CGTCGCTGGA GATGTGCAAG 
ATCACGCTGG AACCGTGTCG GATACTGTAC AACACCAGCT ACTTTCCGGA GTTCCTGAAG 
TGTAACGAAA CGCTGTATCC CTCGAAGTGC AACAACGACG TACGGGAGGA GATGAAGTTC 
AACGCTACCG GGCAGTGTCT CAAACCGCTG GTCGCGGCGG AGTCGCCGGC GAATTACTAT 
AAAGACATCG ACGGATGCGG TCTGCAGTGC AAGGATCCGC TCTACACCGA TCACGAGCAT 
CGACAGATCC ACAAGCTGAT TGCGTGGGGT GCCGGCGTGT GCTTGACGTT CAACTTGTTC 
ACGATCGCCA CGTTTGTGAT CGATTGGCAC AATGCGAACA AGTACCCGGC GTTGGTGATT 
TTCTACATTA ACTTTTGCTT CATGGTTTCG TGCATGGGTT GGCTAGCTCA ATTCACGCCC 
GGAGGTCGAG AGGATATCGT CTGCCGAAAG GATGGAACCT TGCGCATTTC GGAACCCAGT 
GCCGGTGAGA ATCTGTCATG TATTGTCGTG TTTATTCTGG TGTACTATTT CTTGATTGCG 
GCCATGGTTT GGTTCGTCAT TTTTACGTAC GTATGGCATA TGAGTTTCAA AGCTATTGGT 
AAAATTCAGG ATCGAATAGA TAAGAAAGGA TCTTATTTCC ATTTAATTGC TTGGTCACTG 
CCTCTAGTGT TGACGATAAC GATCATGGCT CTGAGCGAGA TCGATGGTAA CAGCACGGTG 
GGAATATGCT TCGTGGGGTA TTTGAATCAC CCGATCAGGG CGGGATTCCT TTTGGGTCCG 
GTGGTATGTG TACTTTTCAT CGGAGGTTAT TTTCTGGGAC GAGGATTGCT AACACTGATA 
AAGCTAAAGA TTTCCAGTAA AGAAATAATT TCGGCTCGTG CAAGTAAGAA AATCAGACAG 
ACGATCGTAC GAATGGGAAT TTGTACGATG TTCACGTTCG TCTTCATTGT TTCGACTATC 
TTCTGTCATA TCCACGAGTT CAAAAACACT GAAAGTTGGA CGATTGCATT GAAGAACTAC 
ATAATTTGTC AGATATCGTC GACTTATACG GATGTGACGA GCACTTGTAA GCTTCATAGC 
CGCCCAAGTG TGGCCGTGCT TCAGATTCAT CTTATTTGCT TGTTTGCGTC CGGAATCGTG 
ATGTCCTCGT GGGTCTGGAC CAACTCCACT CTGGAAACGT GGGGTCGGTA TTTTCGGAGG 
CGCTTCCGAC CAGACGTCGA GGAACCCGTG AAGCTGCAGA AGCATAAGGT TATTGCTCAG 
GCGTTTGCGA AGCGGAAGGA GTTTCAGAAC CAGGGACGAC TCTCGATTTC CTTCCACAAC 
TCCCACACCG ATCCCGTTGG CTTGAAGTTC GATATCAATT CGGCCATCGG GAGTCACGAT 
TTCAGCTCGA CATGGGCGAA TAACCTGCCC AGGTTTGTTA ACAGGCGTTA TGCATTGACG 
GGAGCTGCTA CGAGTTCGAG TCATGATCCG AGAAAGAATT CTGTAGACTC GGAGATAAGC 
TTCAGCGTGC GTCATGTTTC GGTGGAGTCC AGAAGGAACT CGATAGATTC TCAGGTTTCG 
GTAAAGATAG CCGAGATGAA GACCAAGGTC GCAAGCAGGA GTCGCAGTGG AGTTAGTAGC 
AGCAAGCATC ATCATAAGAA TCGCCAACAG AGGCGGCGAG ACTTCACTGC TTCCGGCAGA 
AGATACAGTC GAAAAGAAAG TAGCACTTCG GTAGAATCTC AAATAATAAC GGCAATCCAG 
AAGGGCAGAC ATTCCTCTAG TGGAAACAGT CAATTTTTCG GGGCTAACAA TATGCAACGG 
AGAACTGGTA TCGGTGGTAT GGATGCTGAC CAACTCAACG ATTTTATTTC CAACGGAAAA 
CTATTGCTTC CATTCCTGCA TCAGCCCGGC TTGACGACTT CAGAAGACGA TAACGTCTCA 
AATGCATCAT TCAAGGTTCA GGATTCAAAG TTCGATGTAA TTCTGAAGCA TATCGGAAGT 
GCGGGGACAT CGAGCCGGGT TGATTCACAT AACGCAGGCT GCCAAATCGA AGAATACCAT 
TCCAGTGACG ACGATGAAAA GCTAAGCTTG AAGCAAGCCA GCAGAGACGA ATTCTCCACG 
ATGAAAGATG GCATGAAGAG CAGTAAGGAA GGTGGACGAG GCAGTAAAAA CAGTTCCAAA 
AGTGTTCGCA GTAAGAAATC ATCATCCCGC CAGGGTACCA TTAGAAAGTC CCAAAAGCAT 
TCACGAGCCA GTCAGAAAAG CCAGCGGAAT GAAAAGAAGG AAAAAGCTCG GGAACGAGAA 
CGCGAAAAGG AACGAGAGAA AGAGCGGGAA AAAGAGCTGC AACTCATCGA GCGAAACAAC 
ATTACCGATT TCAACGATTC CTCCTTCACT TCATACTGCT CGGAACTGTC CCCTCTCAAT 
GTGAACAGTT CCTACTCGGG AATCTCACTC GCCAAAACGA ACTCTCGTAA TTCCAAACGT 
AGCTGCGATG TTGGAATTCA GGCGAATGCT TACGAAATCG CCACCCAAAC CATGTCTTCG 
TTCGAGTTCA ACGAAAAAGC GCTGAAAAAT GAAGAGAACG AAGACATCTA CACAGAAAAC 
CACCAGCTGC TAGGGCCCAA ATTGGTACCC CAAGTAGTGC AAGCCCCACG CAAGCGTGAC 
CTCGCGGACG TAAGCTTAAC CGAGGCGGAG AAACTCAAAC AGTTGCTACT TCCCTCAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5568238 ( XM_001657877.3 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001657927.2 Aedes aegypti protein smoothened (LOC5568238), mRNA.

Price & Availability↑

CloneID	OAe14487
Clone ID Related Accession (Same CDS sequence)	XM_001657877.2 , XM_001657877.3
Accession Version	XM_001657877.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	protein smoothened
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001657877.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCCC AATCACTGTT GTCGTCGGTG ACCCTGCTGG TTCTGTTTGG AATCACTGCC 
AACGCGAAAG AATTCACCCC GTCCACGAAT CTCATCACAC CTGTGGACTC GCCCCGACTC 
GAGGCCATCA ATGGAACCCG AAATTTCCGC ATGCACGGTA AGAAAGGCAA GGATGACAAA 
ACCTGGTTCG ATGGCCGCGA GATGCGTTAC ATCTACTGCG TGCGACCGGC CAAGTGCGAA 
CCGATTCGGC ACAAGTCCTG TCTGGGCTCC TCACTGCCGT ACTCCTCCAT CAGTCTGGAT 
CTGACCGATT CCTTCAGCCA GGAGCAAACC CACGATAAAC TGTACCAGTA CAATGCGTTG 
CGTCACGTCC CGAAGTGCTG GGCCGTTATT CAGCCATTTT TGTGCGCCGT GTTCACACCC 
AAGTGCGAAA AGATCAACGG TCGCGACATG GTCTACCTTC CGTCGCTGGA GATGTGCAAG 
ATCACGCTGG AACCGTGTCG GATACTGTAC AACACCAGCT ACTTTCCGGA GTTCCTGAAG 
TGTAACGAAA CGCTGTATCC CTCGAAGTGC AACAACGACG TACGGGAGGA GATGAAGTTC 
AACGCTACCG GGCAGTGTCT CAAACCGCTG GTCGCGGCGG AGTCGCCGGC GAATTACTAT 
AAAGACATCG ACGGATGCGG TCTGCAGTGC AAGGATCCGC TCTACACCGA TCACGAGCAT 
CGACAGATCC ACAAGCTGAT TGCGTGGGGT GCCGGCGTGT GCTTGACGTT CAACTTGTTC 
ACGATCGCCA CGTTTGTGAT CGATTGGCAC AATGCGAACA AGTACCCGGC GTTGGTGATT 
TTCTACATTA ACTTTTGCTT CATGGTTTCG TGCATGGGTT GGCTAGCTCA ATTCACGCCC 
GGAGGTCGAG AGGATATCGT CTGCCGAAAG GATGGAACCT TGCGCATTTC GGAACCCAGT 
GCCGGTGAGA ATCTGTCATG TATTGTCGTG TTTATTCTGG TGTACTATTT CTTGATTGCG 
GCCATGGTTT GGTTCGTCAT TTTTACGTAC GTATGGCATA TGAGTTTCAA AGCTATTGGT 
AAAATTCAGG ATCGAATAGA TAAGAAAGGA TCTTATTTCC ATTTAATTGC TTGGTCACTG 
CCTCTAGTGT TGACGATAAC GATCATGGCT CTGAGCGAGA TCGATGGTAA CAGCACGGTG 
GGAATATGCT TCGTGGGGTA TTTGAATCAC CCGATCAGGG CGGGATTCCT TTTGGGTCCG 
GTGGTATGTG TACTTTTCAT CGGAGGTTAT TTTCTGGGAC GAGGATTGCT AACACTGATA 
AAGCTAAAGA TTTCCAGTAA AGAAATAATT TCGGCTCGTG CAAGTAAGAA AATCAGACAG 
ACGATCGTAC GAATGGGAAT TTGTACGATG TTCACGTTCG TCTTCATTGT TTCGACTATC 
TTCTGTCATA TCCACGAGTT CAAAAACACT GAAAGTTGGA CGATTGCATT GAAGAACTAC 
ATAATTTGTC AGATATCGTC GACTTATACG GATGTGACGA GCACTTGTAA GCTTCATAGC 
CGCCCAAGTG TGGCCGTGCT TCAGATTCAT CTTATTTGCT TGTTTGCGTC CGGAATCGTG 
ATGTCCTCGT GGGTCTGGAC CAACTCCACT CTGGAAACGT GGGGTCGGTA TTTTCGGAGG 
CGCTTCCGAC CAGACGTCGA GGAACCCGTG AAGCTGCAGA AGCATAAGGT TATTGCTCAG 
GCGTTTGCGA AGCGGAAGGA GTTTCAGAAC CAGGGACGAC TCTCGATTTC CTTCCACAAC 
TCCCACACCG ATCCCGTTGG CTTGAAGTTC GATATCAATT CGGCCATCGG GAGTCACGAT 
TTCAGCTCGA CATGGGCGAA TAACCTGCCC AGGTTTGTTA ACAGGCGTTA TGCATTGACG 
GGAGCTGCTA CGAGTTCGAG TCATGATCCG AGAAAGAATT CTGTAGACTC GGAGATAAGC 
TTCAGCGTGC GTCATGTTTC GGTGGAGTCC AGAAGGAACT CGATAGATTC TCAGGTTTCG 
GTAAAGATAG CCGAGATGAA GACCAAGGTC GCAAGCAGGA GTCGCAGTGG AGTTAGTAGC 
AGCAAGCATC ATCATAAGAA TCGCCAACAG AGGCGGCGAG ACTTCACTGC TTCCGGCAGA 
AGATACAGTC GAAAAGAAAG TAGCACTTCG GTAGAATCTC AAATAATAAC GGCAATCCAG 
AAGGGCAGAC ATTCCTCTAG TGGAAACAGT CAATTTTTCG GGGCTAACAA TATGCAACGG 
AGAACTGGTA TCGGTGGTAT GGATGCTGAC CAACTCAACG ATTTTATTTC CAACGGAAAA 
CTATTGCTTC CATTCCTGCA TCAGCCCGGC TTGACGACTT CAGAAGACGA TAACGTCTCA 
AATGCATCAT TCAAGGTTCA GGATTCAAAG TTCGATGTAA TTCTGAAGCA TATCGGAAGT 
GCGGGGACAT CGAGCCGGGT TGATTCACAT AACGCAGGCT GCCAAATCGA AGAATACCAT 
TCCAGTGACG ACGATGAAAA GCTAAGCTTG AAGCAAGCCA GCAGAGACGA ATTCTCCACG 
ATGAAAGATG GCATGAAGAG CAGTAAGGAA GGTGGACGAG GCAGTAAAAA CAGTTCCAAA 
AGTGTTCGCA GTAAGAAATC ATCATCCCGC CAGGGTACCA TTAGAAAGTC CCAAAAGCAT 
TCACGAGCCA GTCAGAAAAG CCAGCGGAAT GAAAAGAAGG AAAAAGCTCG GGAACGAGAA 
CGCGAAAAGG AACGAGAGAA AGAGCGGGAA AAAGAGCTGC AACTCATCGA GCGAAACAAC 
ATTACCGATT TCAACGATTC CTCCTTCACT TCATACTGCT CGGAACTGTC CCCTCTCAAT 
GTGAACAGTT CCTACTCGGG AATCTCACTC GCCAAAACGA ACTCTCGTAA TTCCAAACGT 
AGCTGCGATG TTGGAATTCA GGCGAATGCT TACGAAATCG CCACCCAAAC CATGTCTTCG 
TTCGAGTTCA ACGAAAAAGC GCTGAAAAAT GAAGAGAACG AAGACATCTA CACAGAAAAC 
CACCAGCTGC TAGGGCCCAA ATTGGTACCC CAAGTAGTGC AAGCCCCACG CAAGCGTGAC 
CTCGCGGACG TAAGCTTAAC CGAGGCGGAG AAACTCAAAC AGTTGCTACT TCCCTCAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001657927.2
CDS	1043..4225
Translation	MGAQSLLSSVTLLVLFGITANAKEFTPSTNLITPVDSPRLEAINGTRNFRMHGKKGKDDKTWFDGREMRYIYCVRPAKCEPIRHKSCLGSSLPYSSISLDLTDSFSQEQTHDKLYQYNALRHVPKCWAVIQPFLCAVFTPKCEKINGRDMVYLPSLEMCKITLEPCRILYNTSYFPEFLKCNETLYPSKCNNDVREEMKFNATGQCLKPLVAAESPANYYKDIDGCGLQCKDPLYTDHEHRQIHKLIAWGAGVCLTFNLFTIATFVIDWHNANKYPALVIFYINFCFMVSCMGWLAQFTPGGREDIVCRKDGTLRISEPSAGENLSCIVVFILVYYFLIAAMVWFVIFTYVWHMSFKAIGKIQDRIDKKGSYFHLIAWSLPLVLTITIMALSEIDGNSTVGICFVGYLNHPIRAGFLLGPVVCVLFIGGYFLGRGLLTLIKLKISSKEIISARASKKIRQTIVRMGICTMFTFVFIVSTIFCHIHEFKNTESWTIALKNYIICQISSTYTDVTSTCKLHSRPSVAVLQIHLICLFASGIVMSSWVWTNSTLETWGRYFRRRFRPDVEEPVKLQKHKVIAQAFAKRKEFQNQGRLSISFHNSHTDPVGLKFDINSAIGSHDFSSTWANNLPRFVNRRYALTGAATSSSHDPRKNSVDSEISFSVRHVSVESRRNSIDSQVSVKIAEMKTKVASRSRSGVSSSKHHHKNRQQRRRDFTASGRRYSRKESSTSVESQIITAIQKGRHSSSGNSQFFGANNMQRRTGIGGMDADQLNDFISNGKLLLPFLHQPGLTTSEDDNVSNASFKVQDSKFDVILKHIGSAGTSSRVDSHNAGCQIEEYHSSDDDEKLSLKQASRDEFSTMKDGMKSSKEGGRGSKNSSKSVRSKKSSSRQGTIRKSQKHSRASQKSQRNEKKEKAREREREKEREKEREKELQLIERNNITDFNDSSFTSYCSELSPLNVNSSYSGISLAKTNSRNSKRSCDVGIQANAYEIATQTMSSFEFNEKALKNEENEDIYTENHQLLGPKLVPQVVQAPRKRDLADVSLTEAEKLKQLLLPSK

Target ORF information:

RefSeq Version	XM_001657877.3
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti protein smoothened (LOC5568238), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001657877.3

ORF Insert Sequence:

ATGGGAGCCC AATCACTGTT GTCGTCGGTG ACCCTGCTGG TTCTGTTTGG AATCACTGCC 
AACGCGAAAG AATTCACCCC GTCCACGAAT CTCATCACAC CTGTGGACTC GCCCCGACTC 
GAGGCCATCA ATGGAACCCG AAATTTCCGC ATGCACGGTA AGAAAGGCAA GGATGACAAA 
ACCTGGTTCG ATGGCCGCGA GATGCGTTAC ATCTACTGCG TGCGACCGGC CAAGTGCGAA 
CCGATTCGGC ACAAGTCCTG TCTGGGCTCC TCACTGCCGT ACTCCTCCAT CAGTCTGGAT 
CTGACCGATT CCTTCAGCCA GGAGCAAACC CACGATAAAC TGTACCAGTA CAATGCGTTG 
CGTCACGTCC CGAAGTGCTG GGCCGTTATT CAGCCATTTT TGTGCGCCGT GTTCACACCC 
AAGTGCGAAA AGATCAACGG TCGCGACATG GTCTACCTTC CGTCGCTGGA GATGTGCAAG 
ATCACGCTGG AACCGTGTCG GATACTGTAC AACACCAGCT ACTTTCCGGA GTTCCTGAAG 
TGTAACGAAA CGCTGTATCC CTCGAAGTGC AACAACGACG TACGGGAGGA GATGAAGTTC 
AACGCTACCG GGCAGTGTCT CAAACCGCTG GTCGCGGCGG AGTCGCCGGC GAATTACTAT 
AAAGACATCG ACGGATGCGG TCTGCAGTGC AAGGATCCGC TCTACACCGA TCACGAGCAT 
CGACAGATCC ACAAGCTGAT TGCGTGGGGT GCCGGCGTGT GCTTGACGTT CAACTTGTTC 
ACGATCGCCA CGTTTGTGAT CGATTGGCAC AATGCGAACA AGTACCCGGC GTTGGTGATT 
TTCTACATTA ACTTTTGCTT CATGGTTTCG TGCATGGGTT GGCTAGCTCA ATTCACGCCC 
GGAGGTCGAG AGGATATCGT CTGCCGAAAG GATGGAACCT TGCGCATTTC GGAACCCAGT 
GCCGGTGAGA ATCTGTCATG TATTGTCGTG TTTATTCTGG TGTACTATTT CTTGATTGCG 
GCCATGGTTT GGTTCGTCAT TTTTACGTAC GTATGGCATA TGAGTTTCAA AGCTATTGGT 
AAAATTCAGG ATCGAATAGA TAAGAAAGGA TCTTATTTCC ATTTAATTGC TTGGTCACTG 
CCTCTAGTGT TGACGATAAC GATCATGGCT CTGAGCGAGA TCGATGGTAA CAGCACGGTG 
GGAATATGCT TCGTGGGGTA TTTGAATCAC CCGATCAGGG CGGGATTCCT TTTGGGTCCG 
GTGGTATGTG TACTTTTCAT CGGAGGTTAT TTTCTGGGAC GAGGATTGCT AACACTGATA 
AAGCTAAAGA TTTCCAGTAA AGAAATAATT TCGGCTCGTG CAAGTAAGAA AATCAGACAG 
ACGATCGTAC GAATGGGAAT TTGTACGATG TTCACGTTCG TCTTCATTGT TTCGACTATC 
TTCTGTCATA TCCACGAGTT CAAAAACACT GAAAGTTGGA CGATTGCATT GAAGAACTAC 
ATAATTTGTC AGATATCGTC GACTTATACG GATGTGACGA GCACTTGTAA GCTTCATAGC 
CGCCCAAGTG TGGCCGTGCT TCAGATTCAT CTTATTTGCT TGTTTGCGTC CGGAATCGTG 
ATGTCCTCGT GGGTCTGGAC CAACTCCACT CTGGAAACGT GGGGTCGGTA TTTTCGGAGG 
CGCTTCCGAC CAGACGTCGA GGAACCCGTG AAGCTGCAGA AGCATAAGGT TATTGCTCAG 
GCGTTTGCGA AGCGGAAGGA GTTTCAGAAC CAGGGACGAC TCTCGATTTC CTTCCACAAC 
TCCCACACCG ATCCCGTTGG CTTGAAGTTC GATATCAATT CGGCCATCGG GAGTCACGAT 
TTCAGCTCGA CATGGGCGAA TAACCTGCCC AGGTTTGTTA ACAGGCGTTA TGCATTGACG 
GGAGCTGCTA CGAGTTCGAG TCATGATCCG AGAAAGAATT CTGTAGACTC GGAGATAAGC 
TTCAGCGTGC GTCATGTTTC GGTGGAGTCC AGAAGGAACT CGATAGATTC TCAGGTTTCG 
GTAAAGATAG CCGAGATGAA GACCAAGGTC GCAAGCAGGA GTCGCAGTGG AGTTAGTAGC 
AGCAAGCATC ATCATAAGAA TCGCCAACAG AGGCGGCGAG ACTTCACTGC TTCCGGCAGA 
AGATACAGTC GAAAAGAAAG TAGCACTTCG GTAGAATCTC AAATAATAAC GGCAATCCAG 
AAGGGCAGAC ATTCCTCTAG TGGAAACAGT CAATTTTTCG GGGCTAACAA TATGCAACGG 
AGAACTGGTA TCGGTGGTAT GGATGCTGAC CAACTCAACG ATTTTATTTC CAACGGAAAA 
CTATTGCTTC CATTCCTGCA TCAGCCCGGC TTGACGACTT CAGAAGACGA TAACGTCTCA 
AATGCATCAT TCAAGGTTCA GGATTCAAAG TTCGATGTAA TTCTGAAGCA TATCGGAAGT 
GCGGGGACAT CGAGCCGGGT TGATTCACAT AACGCAGGCT GCCAAATCGA AGAATACCAT 
TCCAGTGACG ACGATGAAAA GCTAAGCTTG AAGCAAGCCA GCAGAGACGA ATTCTCCACG 
ATGAAAGATG GCATGAAGAG CAGTAAGGAA GGTGGACGAG GCAGTAAAAA CAGTTCCAAA 
AGTGTTCGCA GTAAGAAATC ATCATCCCGC CAGGGTACCA TTAGAAAGTC CCAAAAGCAT 
TCACGAGCCA GTCAGAAAAG CCAGCGGAAT GAAAAGAAGG AAAAAGCTCG GGAACGAGAA 
CGCGAAAAGG AACGAGAGAA AGAGCGGGAA AAAGAGCTGC AACTCATCGA GCGAAACAAC 
ATTACCGATT TCAACGATTC CTCCTTCACT TCATACTGCT CGGAACTGTC CCCTCTCAAT 
GTGAACAGTT CCTACTCGGG AATCTCACTC GCCAAAACGA ACTCTCGTAA TTCCAAACGT 
AGCTGCGATG TTGGAATTCA GGCGAATGCT TACGAAATCG CCACCCAAAC CATGTCTTCG 
TTCGAGTTCA ACGAAAAAGC GCTGAAAAAT GAAGAGAACG AAGACATCTA CACAGAAAAC 
CACCAGCTGC TAGGGCCCAA ATTGGTACCC CAAGTAGTGC AAGCCCCACG CAAGCGTGAC 
CTCGCGGACG TAAGCTTAAC CGAGGCGGAG AAACTCAAAC AGTTGCTACT TCCCTCAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5568238 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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