AAEL_AAEL006184 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL006184
Entrez Gene ID	5567585
Full Name	RNA polymerase II-associated protein 1
Synonyms	AAEL006184,AAEL017531
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001651778.2	XP_001651828.2	RNA polymerase II-associated protein 1
XM_001651778.1	XP_001651828.1	AAEL006184-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL006184 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL006184 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAe03509	XM_001651778.1 Latest version!	Aedes aegypti AAEL006184-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OAe129988	XM_001651778.2 Latest version!	Aedes aegypti RNA polymerase II-associated protein 1 (LOC5567585), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL006184 ( XM_001651778.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001651828.1 Aedes aegypti AAEL006184-RA partial mRNA.

Price & Availability↑

CloneID	OAe03509
Clone ID Related Accession (Same CDS sequence)	XM_001651778.1
Accession Version	XM_001651778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL006184-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810736). COMPLETENESS: incomplete on both ends.

ATGTTTATAC CGGGTTTTCC CCATTTTATA TTCTCCAACA GCGATTTACA TCCGGCGCTT 
TTCTATCTGC TAGAGTGGCA CTACCAGCAT CTCGTCCTGG ATGAAAACGG TCCGTTCATC 
ATCCGTCAGC ACTGTGCGTC CTTGTTAGCG CTAATTGGCT ACGACCGGGT TCAGCATGTC 
GCCAACGGAT TCAGTCCGCA GAACTGGGAT CGTTTGTTTG CCTGCTTCAG CAAGTGGTTC 
AACACGGCGG CCAAGTACGG TGTTAATGAG TTTTCCCAGA AGCTGCTACT GGGGGCCTGT 
CTGGATTTGG CGGGCAAGGT GCGAACCATC GCCGGCAGCT ATTACCGGAA CTTTTTCGAC 
GGTTACTTTG GCGCCTTTCT CAAGAGCAGC AAGTTTGACG ACCTGATGAA ACAACTGAGC 
CACTCGCCAC TGCTCGGGAC CAGCCCAGGC ATGGAGGACC GCAGCGCTAA AATCGTGCCC 
CTTCCCAACC TGGGGGGTGT GTTGACGCGC GACAACCCAC CCACGGCTCC CACGCTAATC 
CTGACGAAAT CCTATCCAGT TTTTATGCTG CACGCAATGC TCGGTCTGCT ACGTTCGCAT 
GCCGAGTACG ACCAAACCCC GCTGCGGAAC TTCGTAAACA ACAATTGTCA CTTCAATTAT 
CTGATCAAAC TTGCTGCTAT GGTTTCGGGA CAAAATCGTA AAACCGTTCC GGCGGCGGTC 
GACAAATCAA CCAATTGGTT TTTGAAAAGC GAACTGAATT ATCTGCTCGA TGTGCTGACA 
GTGCTGGTAA AGGATGACCG ATATCATCGT CGACGCGTCA GCGATGAAGG ATCAGGCCAT 
GGGGAGGGTG AAGTTGACAT GGAGAACCGA GGCGGACAAT TGATTTCCAA CGAAGCGACA 
CAAACCAACG ATGTGGGCAA CGATGGTCGA TTATTGCTGG CAGTAACTTT TAAGGTGCTT 
CTGCATCTGA GTGAGGAATT CTATACTGCC ATCGTAAAGC TATTCGATGA GATAGTCTTT 
GTTCGAGATT TCTATCCCAG TCAATGGCTT CAGGAAGGCA ACGCAACTGT CACAGCCGAG 
GAGCTCCAGA GGTGGAAATT CAACTACAAA GTAAAACTAA AATCGTTTCT GAAACGAGAG 
GAAAACTCAT CTGACCGTCG TAACAATTAC ACAGTAACCG AGTGGCGCAC TCCGCTCCTC 
GCCCCAACGT GGCCCTACTC TCTGTTGTAC CTTCTCCTGG AAAAGGCAGA GCAAGGATCC 
GCCAAACTGG CGCACTATTC CGAGGAGCAA ATCATCAGAA CGTCGCTACA ATTCTCTGAA 
TTGTTGGAAC AAAACAGCAT CGTGCTGGCG AGTGTCACCC ACAGACTTAT GTATCTGATG 
ACCGCCTTTC TGGGACCGGA TGCCAAGTTC CTTGACAAGG ACATTTCAGC ACTCATCACA 
CGTCGGATCG ATGCCTTGCG GAACGAGATG CTACTATGCG GGGAACGGTT CGACTTTGAT 
GTCCGGCTCG AGGAGCGAAA GAGCTTCCAC GGGTTGTATC AACTGGTGTT GGACATTTTC 
CAGAGCTCCA GCTACGGTCA CGCACCGTTC AGTGCTTTGG TGATGGCCCC ACTGGCCCAG 
CACTACGACA TTCAGTGGCG CAACCTCGTG TGGTCGGAGC ACGTGGCCGT TCTGAGGTTT 
ATTACCTGCA GCGAGCAACA GCTCTTCGGG ACAATGGACG ACTATTTGCA ACCGGAAGAG 
TCCGATGTCA CCCTGCTCAA GTTCTACAGC CAAGCACTGA ACTCGAATCT GTTGCGCACG 
GGGTCGATTC CCGCCCGGAT AGCGGCTCAC CACGTTCAGG CGTACCGAAC AAAGGCGCAT 
GAAAGGAAAC ATTCTAATGA TACTGATAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001651828.1
CDS	1..1893
Translation	MFIPGFPHFIFSNSDLHPALFYLLEWHYQHLVLDENGPFIIRQHCASLLALIGYDRVQHVANGFSPQNWDRLFACFSKWFNTAAKYGVNEFSQKLLLGACLDLAGKVRTIAGSYYRNFFDGYFGAFLKSSKFDDLMKQLSHSPLLGTSPGMEDRSAKIVPLPNLGGVLTRDNPPTAPTLILTKSYPVFMLHAMLGLLRSHAEYDQTPLRNFVNNNCHFNYLIKLAAMVSGQNRKTVPAAVDKSTNWFLKSELNYLLDVLTVLVKDDRYHRRRVSDEGSGHGEGEVDMENRGGQLISNEATQTNDVGNDGRLLLAVTFKVLLHLSEEFYTAIVKLFDEIVFVRDFYPSQWLQEGNATVTAEELQRWKFNYKVKLKSFLKREENSSDRRNNYTVTEWRTPLLAPTWPYSLLYLLLEKAEQGSAKLAHYSEEQIIRTSLQFSELLEQNSIVLASVTHRLMYLMTAFLGPDAKFLDKDISALITRRIDALRNEMLLCGERFDFDVRLEERKSFHGLYQLVLDIFQSSSYGHAPFSALVMAPLAQHYDIQWRNLVWSEHVAVLRFITCSEQQLFGTMDDYLQPEESDVTLLKFYSQALNSNLLRTGSIPARIAAHHVQAYRTKAHERKHSNDTDK

Target ORF information:

RefSeq Version	XM_001651778.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL006184-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001651778.1

ORF Insert Sequence:

ATGTTTATAC CGGGTTTTCC CCATTTTATA TTCTCCAACA GCGATTTACA TCCGGCGCTT 
TTCTATCTGC TAGAGTGGCA CTACCAGCAT CTCGTCCTGG ATGAAAACGG TCCGTTCATC 
ATCCGTCAGC ACTGTGCGTC CTTGTTAGCG CTAATTGGCT ACGACCGGGT TCAGCATGTC 
GCCAACGGAT TCAGTCCGCA GAACTGGGAT CGTTTGTTTG CCTGCTTCAG CAAGTGGTTC 
AACACGGCGG CCAAGTACGG TGTTAATGAG TTTTCCCAGA AGCTGCTACT GGGGGCCTGT 
CTGGATTTGG CGGGCAAGGT GCGAACCATC GCCGGCAGCT ATTACCGGAA CTTTTTCGAC 
GGTTACTTTG GCGCCTTTCT CAAGAGCAGC AAGTTTGACG ACCTGATGAA ACAACTGAGC 
CACTCGCCAC TGCTCGGGAC CAGCCCAGGC ATGGAGGACC GCAGCGCTAA AATCGTGCCC 
CTTCCCAACC TGGGGGGTGT GTTGACGCGC GACAACCCAC CCACGGCTCC CACGCTAATC 
CTGACGAAAT CCTATCCAGT TTTTATGCTG CACGCAATGC TCGGTCTGCT ACGTTCGCAT 
GCCGAGTACG ACCAAACCCC GCTGCGGAAC TTCGTAAACA ACAATTGTCA CTTCAATTAT 
CTGATCAAAC TTGCTGCTAT GGTTTCGGGA CAAAATCGTA AAACCGTTCC GGCGGCGGTC 
GACAAATCAA CCAATTGGTT TTTGAAAAGC GAACTGAATT ATCTGCTCGA TGTGCTGACA 
GTGCTGGTAA AGGATGACCG ATATCATCGT CGACGCGTCA GCGATGAAGG ATCAGGCCAT 
GGGGAGGGTG AAGTTGACAT GGAGAACCGA GGCGGACAAT TGATTTCCAA CGAAGCGACA 
CAAACCAACG ATGTGGGCAA CGATGGTCGA TTATTGCTGG CAGTAACTTT TAAGGTGCTT 
CTGCATCTGA GTGAGGAATT CTATACTGCC ATCGTAAAGC TATTCGATGA GATAGTCTTT 
GTTCGAGATT TCTATCCCAG TCAATGGCTT CAGGAAGGCA ACGCAACTGT CACAGCCGAG 
GAGCTCCAGA GGTGGAAATT CAACTACAAA GTAAAACTAA AATCGTTTCT GAAACGAGAG 
GAAAACTCAT CTGACCGTCG TAACAATTAC ACAGTAACCG AGTGGCGCAC TCCGCTCCTC 
GCCCCAACGT GGCCCTACTC TCTGTTGTAC CTTCTCCTGG AAAAGGCAGA GCAAGGATCC 
GCCAAACTGG CGCACTATTC CGAGGAGCAA ATCATCAGAA CGTCGCTACA ATTCTCTGAA 
TTGTTGGAAC AAAACAGCAT CGTGCTGGCG AGTGTCACCC ACAGACTTAT GTATCTGATG 
ACCGCCTTTC TGGGACCGGA TGCCAAGTTC CTTGACAAGG ACATTTCAGC ACTCATCACA 
CGTCGGATCG ATGCCTTGCG GAACGAGATG CTACTATGCG GGGAACGGTT CGACTTTGAT 
GTCCGGCTCG AGGAGCGAAA GAGCTTCCAC GGGTTGTATC AACTGGTGTT GGACATTTTC 
CAGAGCTCCA GCTACGGTCA CGCACCGTTC AGTGCTTTGG TGATGGCCCC ACTGGCCCAG 
CACTACGACA TTCAGTGGCG CAACCTCGTG TGGTCGGAGC ACGTGGCCGT TCTGAGGTTT 
ATTACCTGCA GCGAGCAACA GCTCTTCGGG ACAATGGACG ACTATTTGCA ACCGGAAGAG 
TCCGATGTCA CCCTGCTCAA GTTCTACAGC CAAGCACTGA ACTCGAATCT GTTGCGCACG 
GGGTCGATTC CCGCCCGGAT AGCGGCTCAC CACGTTCAGG CGTACCGAAC AAAGGCGCAT 
GAAAGGAAAC ATTCTAATGA TACTGATAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL006184 ( XM_001651778.2 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001651828.2 Aedes aegypti RNA polymerase II-associated protein 1 (LOC5567585), mRNA.

Price & Availability↑

CloneID	OAe129988
Clone ID Related Accession (Same CDS sequence)	XM_001651778.2
Accession Version	XM_001651778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	RNA polymerase II-associated protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001651778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAAAA GACCATCCAG CAAGGACAAT GAGAATGATA TCCTGCGCAT GCAGCAGGAT 
TTTTACGCCG AGTCGAATCG AGACACGAGT TTCCAACCGG CAGCCAAAGT GGTTCGTGTT 
CGGCAAGAAG GCAAAACGGA AGGCGCTTCA GCTGGGAAAG TTCCTCGCCA GTCGGAATTT 
GCTCGACGTC GACAGCTCCA GAAAGCCAAC AAAGAGGAGA CCGCACCGGA GCGGAAACCG 
GATGTTCCGG AGCACACAAT CATCGGTGAC ATTGTGGAGA AGCGCTACGA AGAGGGATTT 
GTTCCGATAG AAGTGGAGGA GAGGGGTGAC TCGGCAAGGG AAGCTTTCCC AAAACCGATA 
CATTTGAAGG TGCTTCCCCA CGGAAGTGGC CAGAAGAAAA GCTTATTCGC TCAGATGTTC 
CAGAAAGAGG CTGGTTCCGA TTCCGGTGGT TCGGAGCGTA AGAGTTCAAA GGTTCATCTT 
CCGACTGAAA GCGTGATTCT TGAGGGAAGC GATGCGATGG ACATCCACGA AGAGAATCTG 
GAAAAGATGA AGCAAATGGA TGAGGGTGAA ATACTGAAGG AAAGAGAACA TCTAATGAGC 
ACTATGGATC CCAAACTGGT AGAGTTCTTG AAGGCCAGGA AGAAGGGTCC GGTTGCAAGC 
CAAAAGAAAG CACCGGAGCC AATTCAGGAA GTGGAAGAGA TAACCACGGA AGCCCCAAAT 
CTGGATGTAT TCTGTCAGGA AGGGTCTGAT CAGTGGATCA ATTTCGATAT TTTGGAACCG 
GAAAAGCTGG AATGGACAAA GAACATCCAA AAAACCATGA AAGATCTTAA ACCGGGTGAA 
ACCTTTGAGG CCCGGTTTGA CTGGAAGGGA GTTCTTCAAC CGTATGTGGT TAAAGAGGAA 
GATCCAAATA AAGATGACCG AGAATTGTAT TTGCATGGGG AAGAAGCAGA TCGTCCGGGT 
TACACCCTGC AGGAGCTATT CCGATTAGCT CGGGCAAACG TTCTTCAGCA ACGAATATCC 
GCTCTGAACG CAATTGGCGG CATTTTGAAC ATTCTCAACC AGGGATTCTA CGACGGAGTG 
CTGGAGCTTC CAATATCCAA GATATTCTTC TTTTTGAGAT TCGCTCTGGA CGAGAACACG 
CCGGCAGTCG TGGAAGCGGC CTCCCGAGCT TTGGCCTACC TTTTCTACAA CGATACGGAT 
GAAACCCTCT TGGATACGAT TTACGACACC AAAAATGGCA TCTACCAGCC GGAACTCGGT 
CTCAACGTTA CTCACAGTCG TACGGAAGAA GATGACCTGC GCCAAAAGGA ACTCGAAACC 
GGATTTGGCT CGTTGAAACT TAACAGCCAA AAGCAATCCC AAAAATCCAC CCGCAAGGTC 
CACTTCTCGG CCGAGCTCTA CGATGACCCG GACGACATCC ACAATCGGGA AACGATGAAC 
GATTTCCACC TGGCGGAGAC CGACCTTCTC GAGTGTCTCC TGCGAACCAA CATTCTGGAA 
CGCATACGCT ATATACTGTT CTCGATGCGT CCCGAGGGAA CCACGGTCAT CAGCTGTGCC 
AAGCTGTTGA TTCGTCTCGC GCGAACAAAC GAATCGATGG CGGTGAAGAT CGCTTCCAAT 
GAGTTGCTGA TGGGGGGTTT GATAGGTGGT TACCTCAGTT CTTCCGTTGA AGGCCTGGAG 
GGGAAGCAAC TGCCGCCGCA GCACGTGGTC ATAAAGTTGC TACGAGTTTT GTGCTCGTAT 
GGGGGCGAGA TGTACGAAAG ATACCTGGCG AGATACCATG TGACCAGTCT GCTGAAGCGG 
CACGTTTTCA GCCGGAGGGA TATCAATGTA ACATTGATTC AGACCCAAAT TGAAACATTC 
CGTTTCATGC GGTTGATCTT GAAGACTACT CAAAACGATG AATTGTATTG CGATTTACAT 
CCGGCGCTTT TCTATCTGTT AGAGTGGCAC TACCAGCATC TCGTCCTGGA TGAAAACGGT 
CCGTTCATCA TTCGTCAGCA CTGTGCGTCC TTGTTAGCGC TAATTGGCTA CGACCGGGTT 
CAGCATGTCG CCAACGGATT CAGTCCGCAG AACTGGGATC GTTTGTTTGC CTGCTTCAGC 
AAGTGGTTCA ACACGGCGGC CAAGTACGGT GTTAATGAGT TTTCCCAGAA GCTGCTACTG 
GGGGCCTGTC TGGATTTGGC GGGCAAGGTG CGAACCATCG CCGGCAGCTA TTACCGAAAC 
TTTTTCGACG GTTACTTTGG CGCCTTTCTC AAGAGCAGCA AGTTTGACGA CCTGATGAAA 
CAACTGAGCC ACTCGCCACT GCTCGGGACC AGCCCAGGCA TGGAGGACCG CAGCGCTAAA 
ATCGCGCCCC TTCCCAACCT GGGGGGTGTG TTGACGCGCG ACAACCCACC CACGGCTCCC 
ACGCTAATCC TGACGAAATC CTATCCAGTT TTTATGCTGC ACGCAATGCT CGGTCTGCTA 
CGTTCGCATG CCGAGTACGA CCAAACCCCG CTGCGGAACT TCGTGAATAA CAAGTGTCAC 
TTCAATTATC TGATCAAACT TGCTGCTATG GTTTCGGGAC AAAATCGTAA AACCGTTCCG 
GCGGCGGTCG ACAAATCAAC CAATTGGTTT TTGAAAAGCG AACTGAATTA TCTGCTCGAT 
GTGCTGACAG TGCTGGTAAA GGATGACCGA TATCATCGTC GACGCGTCAG CGATGAAGGA 
TCAGGCCATG GGGAGGGTGA CGTTGACATG GAGAACCGAG GCGGACAATT GATTTCCAAC 
GAAGCGACAC AAACCAACGA TGTGGGCAAC GATGGTCGAT TATTGCTGGC AGTAACTTTT 
AAGGTGCTTC TGCATCTGAG TGAGGAATTC TATACTGCCA TCGTAAAGCT ATTCGATGAG 
ATAGTCTTTG TTCGAGATTT CTATCCCAGT CAATGGCTTC AGGAAGGCAA CGCAACTGTC 
ACAGCCGAGG AGCTCCAGAG GTGGAAATTC AACTACAAAG TAAAACTAAA ATCGTTTCTG 
AAACGAGAGG AAAACTCATC TGACCGTCGT AAAAATTACA CAGTAACCGA GTGGCGCACT 
CCGCTCCTCG CCCCAACGTG GCCCTACTCT CTGTTGTACC TTCTCCTGGA AAAGGCAGAG 
CAAGGATCCG CCAAACTGGC GCACTATTCC GAGGAGCAAA TCATCAGAAC GTCGCTACAA 
TTTTCTGAAT TGTTGGAACA AAACAGCATC GTGCTGGCGA GTGTCACCCA CAGACTTATG 
TATCTGATGA CCGCCTTTCT GGGACCGGAT GCCAAGTTCC TTGACAAGCA CATTTCAGCA 
CTCATCACAC GTCGGATCGA TGCCTTGCGG AACGATATGC TACTAAGCGG GGAACGGTTC 
GACTTTGATG TCCGGCTCGA GGAGCGAAAG AGCTTCCACG GGTTGTATCA ACTAGTGTTG 
GACATTTTCC AGAGCTCCAG CTACGGTCAC GCACCGTTCA GTGCTTTGGT GATGGCCCCA 
CTGGCCCAGC ACTACGACAT TCAGTGGCGC AACCTCGTGT GGTCGGAGCA CGTGGCCGTT 
CTGAGGTTTA TTACCTGCAG CGAGCAACAG CTCTTCGGGA CAATGGACGA CTATTTGCAA 
CCGGAAGAGT CCGATGTCAC CCTGCTCAAG TTCTACAGCC AAGCACTGAA CTCGAATCTG 
TTGCGCACGG GGTCGATTCC CGCCCGGATA GCGGCTCACC ACGTTCAGGC GTACCGAACA 
AAGGCGCATG AAAGGAAACA TTCTAATGAT ACTGATAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001651828.2
CDS	121..3882
Translation	MIKRPSSKDNENDILRMQQDFYAESNRDTSFQPAAKVVRVRQEGKTEGASAGKVPRQSEFARRRQLQKANKEETAPERKPDVPEHTIIGDIVEKRYEEGFVPIEVEERGDSAREAFPKPIHLKVLPHGSGQKKSLFAQMFQKEAGSDSGGSERKSSKVHLPTESVILEGSDAMDIHEENLEKMKQMDEGEILKEREHLMSTMDPKLVEFLKARKKGPVASQKKAPEPIQEVEEITTEAPNLDVFCQEGSDQWINFDILEPEKLEWTKNIQKTMKDLKPGETFEARFDWKGVLQPYVVKEEDPNKDDRELYLHGEEADRPGYTLQELFRLARANVLQQRISALNAIGGILNILNQGFYDGVLELPISKIFFFLRFALDENTPAVVEAASRALAYLFYNDTDETLLDTIYDTKNGIYQPELGLNVTHSRTEEDDLRQKELETGFGSLKLNSQKQSQKSTRKVHFSAELYDDPDDIHNRETMNDFHLAETDLLECLLRTNILERIRYILFSMRPEGTTVISCAKLLIRLARTNESMAVKIASNELLMGGLIGGYLSSSVEGLEGKQLPPQHVVIKLLRVLCSYGGEMYERYLARYHVTSLLKRHVFSRRDINVTLIQTQIETFRFMRLILKTTQNDELYCDLHPALFYLLEWHYQHLVLDENGPFIIRQHCASLLALIGYDRVQHVANGFSPQNWDRLFACFSKWFNTAAKYGVNEFSQKLLLGACLDLAGKVRTIAGSYYRNFFDGYFGAFLKSSKFDDLMKQLSHSPLLGTSPGMEDRSAKIAPLPNLGGVLTRDNPPTAPTLILTKSYPVFMLHAMLGLLRSHAEYDQTPLRNFVNNKCHFNYLIKLAAMVSGQNRKTVPAAVDKSTNWFLKSELNYLLDVLTVLVKDDRYHRRRVSDEGSGHGEGDVDMENRGGQLISNEATQTNDVGNDGRLLLAVTFKVLLHLSEEFYTAIVKLFDEIVFVRDFYPSQWLQEGNATVTAEELQRWKFNYKVKLKSFLKREENSSDRRKNYTVTEWRTPLLAPTWPYSLLYLLLEKAEQGSAKLAHYSEEQIIRTSLQFSELLEQNSIVLASVTHRLMYLMTAFLGPDAKFLDKHISALITRRIDALRNDMLLSGERFDFDVRLEERKSFHGLYQLVLDIFQSSSYGHAPFSALVMAPLAQHYDIQWRNLVWSEHVAVLRFITCSEQQLFGTMDDYLQPEESDVTLLKFYSQALNSNLLRTGSIPARIAAHHVQAYRTKAHERKHSNDTDK

Target ORF information:

RefSeq Version	XM_001651778.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti RNA polymerase II-associated protein 1 (LOC5567585), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001651778.2

ORF Insert Sequence:

ATGATCAAAA GACCATCCAG CAAGGACAAT GAGAATGATA TCCTGCGCAT GCAGCAGGAT 
TTTTACGCCG AGTCGAATCG AGACACGAGT TTCCAACCGG CAGCCAAAGT GGTTCGTGTT 
CGGCAAGAAG GCAAAACGGA AGGCGCTTCA GCTGGGAAAG TTCCTCGCCA GTCGGAATTT 
GCTCGACGTC GACAGCTCCA GAAAGCCAAC AAAGAGGAGA CCGCACCGGA GCGGAAACCG 
GATGTTCCGG AGCACACAAT CATCGGTGAC ATTGTGGAGA AGCGCTACGA AGAGGGATTT 
GTTCCGATAG AAGTGGAGGA GAGGGGTGAC TCGGCAAGGG AAGCTTTCCC AAAACCGATA 
CATTTGAAGG TGCTTCCCCA CGGAAGTGGC CAGAAGAAAA GCTTATTCGC TCAGATGTTC 
CAGAAAGAGG CTGGTTCCGA TTCCGGTGGT TCGGAGCGTA AGAGTTCAAA GGTTCATCTT 
CCGACTGAAA GCGTGATTCT TGAGGGAAGC GATGCGATGG ACATCCACGA AGAGAATCTG 
GAAAAGATGA AGCAAATGGA TGAGGGTGAA ATACTGAAGG AAAGAGAACA TCTAATGAGC 
ACTATGGATC CCAAACTGGT AGAGTTCTTG AAGGCCAGGA AGAAGGGTCC GGTTGCAAGC 
CAAAAGAAAG CACCGGAGCC AATTCAGGAA GTGGAAGAGA TAACCACGGA AGCCCCAAAT 
CTGGATGTAT TCTGTCAGGA AGGGTCTGAT CAGTGGATCA ATTTCGATAT TTTGGAACCG 
GAAAAGCTGG AATGGACAAA GAACATCCAA AAAACCATGA AAGATCTTAA ACCGGGTGAA 
ACCTTTGAGG CCCGGTTTGA CTGGAAGGGA GTTCTTCAAC CGTATGTGGT TAAAGAGGAA 
GATCCAAATA AAGATGACCG AGAATTGTAT TTGCATGGGG AAGAAGCAGA TCGTCCGGGT 
TACACCCTGC AGGAGCTATT CCGATTAGCT CGGGCAAACG TTCTTCAGCA ACGAATATCC 
GCTCTGAACG CAATTGGCGG CATTTTGAAC ATTCTCAACC AGGGATTCTA CGACGGAGTG 
CTGGAGCTTC CAATATCCAA GATATTCTTC TTTTTGAGAT TCGCTCTGGA CGAGAACACG 
CCGGCAGTCG TGGAAGCGGC CTCCCGAGCT TTGGCCTACC TTTTCTACAA CGATACGGAT 
GAAACCCTCT TGGATACGAT TTACGACACC AAAAATGGCA TCTACCAGCC GGAACTCGGT 
CTCAACGTTA CTCACAGTCG TACGGAAGAA GATGACCTGC GCCAAAAGGA ACTCGAAACC 
GGATTTGGCT CGTTGAAACT TAACAGCCAA AAGCAATCCC AAAAATCCAC CCGCAAGGTC 
CACTTCTCGG CCGAGCTCTA CGATGACCCG GACGACATCC ACAATCGGGA AACGATGAAC 
GATTTCCACC TGGCGGAGAC CGACCTTCTC GAGTGTCTCC TGCGAACCAA CATTCTGGAA 
CGCATACGCT ATATACTGTT CTCGATGCGT CCCGAGGGAA CCACGGTCAT CAGCTGTGCC 
AAGCTGTTGA TTCGTCTCGC GCGAACAAAC GAATCGATGG CGGTGAAGAT CGCTTCCAAT 
GAGTTGCTGA TGGGGGGTTT GATAGGTGGT TACCTCAGTT CTTCCGTTGA AGGCCTGGAG 
GGGAAGCAAC TGCCGCCGCA GCACGTGGTC ATAAAGTTGC TACGAGTTTT GTGCTCGTAT 
GGGGGCGAGA TGTACGAAAG ATACCTGGCG AGATACCATG TGACCAGTCT GCTGAAGCGG 
CACGTTTTCA GCCGGAGGGA TATCAATGTA ACATTGATTC AGACCCAAAT TGAAACATTC 
CGTTTCATGC GGTTGATCTT GAAGACTACT CAAAACGATG AATTGTATTG CGATTTACAT 
CCGGCGCTTT TCTATCTGTT AGAGTGGCAC TACCAGCATC TCGTCCTGGA TGAAAACGGT 
CCGTTCATCA TTCGTCAGCA CTGTGCGTCC TTGTTAGCGC TAATTGGCTA CGACCGGGTT 
CAGCATGTCG CCAACGGATT CAGTCCGCAG AACTGGGATC GTTTGTTTGC CTGCTTCAGC 
AAGTGGTTCA ACACGGCGGC CAAGTACGGT GTTAATGAGT TTTCCCAGAA GCTGCTACTG 
GGGGCCTGTC TGGATTTGGC GGGCAAGGTG CGAACCATCG CCGGCAGCTA TTACCGAAAC 
TTTTTCGACG GTTACTTTGG CGCCTTTCTC AAGAGCAGCA AGTTTGACGA CCTGATGAAA 
CAACTGAGCC ACTCGCCACT GCTCGGGACC AGCCCAGGCA TGGAGGACCG CAGCGCTAAA 
ATCGCGCCCC TTCCCAACCT GGGGGGTGTG TTGACGCGCG ACAACCCACC CACGGCTCCC 
ACGCTAATCC TGACGAAATC CTATCCAGTT TTTATGCTGC ACGCAATGCT CGGTCTGCTA 
CGTTCGCATG CCGAGTACGA CCAAACCCCG CTGCGGAACT TCGTGAATAA CAAGTGTCAC 
TTCAATTATC TGATCAAACT TGCTGCTATG GTTTCGGGAC AAAATCGTAA AACCGTTCCG 
GCGGCGGTCG ACAAATCAAC CAATTGGTTT TTGAAAAGCG AACTGAATTA TCTGCTCGAT 
GTGCTGACAG TGCTGGTAAA GGATGACCGA TATCATCGTC GACGCGTCAG CGATGAAGGA 
TCAGGCCATG GGGAGGGTGA CGTTGACATG GAGAACCGAG GCGGACAATT GATTTCCAAC 
GAAGCGACAC AAACCAACGA TGTGGGCAAC GATGGTCGAT TATTGCTGGC AGTAACTTTT 
AAGGTGCTTC TGCATCTGAG TGAGGAATTC TATACTGCCA TCGTAAAGCT ATTCGATGAG 
ATAGTCTTTG TTCGAGATTT CTATCCCAGT CAATGGCTTC AGGAAGGCAA CGCAACTGTC 
ACAGCCGAGG AGCTCCAGAG GTGGAAATTC AACTACAAAG TAAAACTAAA ATCGTTTCTG 
AAACGAGAGG AAAACTCATC TGACCGTCGT AAAAATTACA CAGTAACCGA GTGGCGCACT 
CCGCTCCTCG CCCCAACGTG GCCCTACTCT CTGTTGTACC TTCTCCTGGA AAAGGCAGAG 
CAAGGATCCG CCAAACTGGC GCACTATTCC GAGGAGCAAA TCATCAGAAC GTCGCTACAA 
TTTTCTGAAT TGTTGGAACA AAACAGCATC GTGCTGGCGA GTGTCACCCA CAGACTTATG 
TATCTGATGA CCGCCTTTCT GGGACCGGAT GCCAAGTTCC TTGACAAGCA CATTTCAGCA 
CTCATCACAC GTCGGATCGA TGCCTTGCGG AACGATATGC TACTAAGCGG GGAACGGTTC 
GACTTTGATG TCCGGCTCGA GGAGCGAAAG AGCTTCCACG GGTTGTATCA ACTAGTGTTG 
GACATTTTCC AGAGCTCCAG CTACGGTCAC GCACCGTTCA GTGCTTTGGT GATGGCCCCA 
CTGGCCCAGC ACTACGACAT TCAGTGGCGC AACCTCGTGT GGTCGGAGCA CGTGGCCGTT 
CTGAGGTTTA TTACCTGCAG CGAGCAACAG CTCTTCGGGA CAATGGACGA CTATTTGCAA 
CCGGAAGAGT CCGATGTCAC CCTGCTCAAG TTCTACAGCC AAGCACTGAA CTCGAATCTG 
TTGCGCACGG GGTCGATTCC CGCCCGGATA GCGGCTCACC ACGTTCAGGC GTACCGAACA 
AAGGCGCATG AAAGGAAACA TTCTAATGAT ACTGATAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL006184 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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