AAEL_AAEL005809 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL005809
Entrez Gene ID	5567088
Full Name	centrosomal protein of 290 kDa
Synonyms	AAEL012315
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021841770.1	XP_021697462.1	LOW QUALITY PROTEIN: centrosomal protein of 290 kDa
XM_001651400.1	XP_001651450.1	AAEL005809-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL005809 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL005809 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAe119749	XM_021841770.1 Latest version!	Aedes aegypti centrosomal protein of 290 kDa (LOC5567088), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL005809 ( XM_021841770.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021697462.1 Aedes aegypti centrosomal protein of 290 kDa (LOC5567088), mRNA.

Price & Availability↑

CloneID	OAe119749
Clone ID Related Accession (Same CDS sequence)	XM_021841770.1
Accession Version	XM_021841770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	LOW QUALITY PROTEIN: centrosomal protein of 290 kDa
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGATTGGC GCTACATGCT GTCCGTGTCG CCGCACAATC TGGCCGACGA GCGCAAGGAT 
GAAGTGGCCG TCCATTTGGC TGCCGTTCCA ACCGACCAGA AGTTGGACAG CAAGAGCTGT 
AAAAAGTTGT TCCGGTTGGC GCAGGTGGTG CTACGCTTTC GCAACGAGCA GGTTGGTCAA 
CTTAGAATCG AAATCGACCG AATGGTGGAA GAGAACGAAC AGCGCGATGC ACGAGGATCA 
CCCTCAGATC AGCTCCGGGA GCAAGTGGCA GACCTCAAGC GAATCATTGA CAAGCTGGAA 
GCAGAACGGC ACACGAGCCG TGCTAGGATC AAGGAGCTAT CTGAAGAAAT GACCTCACTA 
CAGAGAAAGC TAGCGGAAAG CAGTCACTTC GGCAGTGAAG GTGAAGACTC ACCAGACGCG 
CTGTCGGAAA TCGATCGCCA ACAGGAACTG TACAACAACA TCAGTATGAA GAATAAACAC 
ATTAAGCGTT TGCTGCGAGA CTTAGATGAT TTGGAAAAAC GTAACAGCTT CCAGATCGAC 
ACCATCAACA GTTTGCAGGT CAGCCTGAAC GATGCAACAA TGAATTTGAC CGGATTGACT 
CATCAATATG AGGAAGCCCA AGCGATTATG AAGGAGCAGG AAGTTCTGAT TGAAGAATCT 
AATGAGAAAA TCAAACGATT GGAAGAGGAT GTGGAGGGGT TAGCCGAAGA GAAAGTTAGA 
TTTGAGGAAG ATTTGGAAGG GTTTGCGAAG CAGCTAGAAG AACGGACTGA GCAGTGGCAA 
AGTATCCTAG AGAAAAAGCA AGAAGAGTTG GAGGAAATCG AAACAAAATA TTCAGAGTTG 
CTGCAACAGT TTCCTGGGTA TGACATCGAA GCGGAGAGAA AGGAATTCAA AAAGATGAGC 
GCAAGGCTTC AGGAAAAAGA TGAGATGATT GAAGAGTTGG AGAAGAAAAT CACTATGCTA 
TCGCAGGAGA TTCTAACGTC CACGGAGCTT ATGAACAGGA TTTCGCAAGA GAAAGAGAAT 
GCCAGTAAGG ATACGGTGAA GGTAAGTCAG TGTTGCGAGG AAAGCCGTGC GTTGCTGGAG 
AAGGCAAATA AGCGATGCGA AGAGATGCAG GAAATTTTGT CCAATGTGGA GGAGGATAAC 
ATGTTGAAGT CGAAACAGGC GGTCGAAGCG ATCGAGGCAT TGAGAAGATA CGAAAGTGGC 
GAGGAAGGTT TGGCGAATGC ATTGAAGAAG ATCAACAGGT TGCATGAGAA AGTTAACGTA 
CGGGATAAGC AGATACGGGA ATTGGTGGCG GAAATCAATC TGGCGAACGA GATTGCCATG 
GAGAATGCTG TGCTGAGGAA GCGTTTGGGT ATTAGTGAGG ACGAAGTTAT ACCAACTCAT 
TCTGTTCTGT CTAAGCAGAG GAAAATTGAG AAAGTAAACG AACGCTTGGC TTTGAAATTA 
AGAGCATCGG AGGAAATGCG ACTTCAACTG AAGTTGGAGA AGAATGATCT GAAGCGAAAA 
CTTTTTCAGA TGTCACAATC TTTGAGTGGT CAAAGGAGTT TAGACAAGGA AAAAGCGATG 
ATATCAATCA ACAGCAATCA TCGGTGAAGC GCCGTAACGG AAGGAAATCA GGCAACAGAT 
GCAGTCGATG AAATCTCGAA GCTTAGTGAT ACGGATGTTG CGAAGTTCTG TGAGAAATGC 
ATGAAGCAGT ATAACGTTAA TGAGAGTAGA AAGTTTTGCA AGGCGTGCAT CTTTCGGCAG 
AGCTTCAACT ATTGTGATGG ATGCATCAAA AAATTGAAGG GTAATATCAA CTCGGACGAA 
GATCCAAAGA CAAAGTATGC TGAAAAGATT GAAGAATTGG AGAAAAAGTA CGCTGTTGTT 
GTGGAGGAAA ATGAGAACTT GAGAATAGGG ATGCATGAGA TTTTGCAGAA ATTGCGAGAG 
TACGATGCTA CGTCAAATCA TTTAACGATC GACACATCTA CTTTAGAACG ATTGTTGCAT 
GCACTGGATG CCAGAAGCGT GAGTGGATGG TATCACCCGG CTATGCGGTT ACAGAACGAA 
TTGCTGGCCA CGAGAGAAAG GGAAATGCTG CTAAAAGAAC GTTTGAGTCT CAAAAGCAAA 
CAAAGCACAT TTTCACTGGG AAGTCGAAAT GATTCCGGCA ATGTTTGTGA TGATGAGGAA 
AACACAGCGG ATATAGATCA AGTTATAACA GAGGAGCCGG ATTTTGGGGA GCAAGTGCTG 
CTCAAATCGG TGGAAATCGA TCGACTGAAT GATCAAATCG AATCTCTGAA GGAAGAACGA 
GAGCAATTGC TTCAATCAAA TGACGAACTA GTAGTGACGA GAAAGTTGTA TAATGAATTG 
ATGGAATTTA CCAAATCGAC CGATAATGAG AAAGATAGGA TATTGGTTGA AACGCTAGAG 
CGGCTGAAGC TGATAGAATC CAATATATAT GCCTTCCAGA GAAAGATTGA GTTCTTGAAA 
GCCGAGAATG ACAACATGCA CAACACAATG CGGACAATCG AAGTAGAGCA CTTGAACATT 
CTCCACGGGT TGAGATCCGA ATTGGCGCGG AAGAATAGCA CATTGAAAAA GTTGGAACAT 
CAAACCAGCG TTGAGGGAAG GAATGTAAGT AGTCCGGATT CAGAATCGAT CGGGAAGCTA 
GAGTCTGAGA TTGAGCGAAT GAAGCTGGAG ACGGCCAATT TCTATACGAT CTTCTTGAGA 
AACATTCAGG AAGTGGACAA GGACAATCTG TTGCATTTGG AGTATGAGAA TCTAGCTCGC 
GTTGGGTTGG TTGAATCGAA TTTGGCGGTA GATTTTATGC CGAAAGAAGA GTTTAAGAGG 
ATCAAGAAAC AGTTACAGAC AGCACAGGAA GAGTTCAAAA AACAAACTAT TAAGAATGGT 
CACTTGGAAG AACTTCTGAA GATATCACAA GAACAACTTC GGTCACAGCA AATGTTGATT 
TCAAAGTTTT CAGATGAGGA AATCAGTTTG CGGCATTTGG TTGCGGATTT ACAGAGTTCA 
TCAAACGAAA AGTATTTACT GGTCAAAACT CAGAAGGAGC TTGACGCTGC CAAAGAGCAA 
GGAGAGCTTT TTAAATTAAG TAATGCTAAG CTGAAGCAAT CGTTGATTGA CAGCGGCGAG 
GAGTTGAATA AGTTAAAGGA GCAGCTAGCT CAACAAGAGT TAGATTTCAT CGAACATCAG 
AAAGACAACG AGCTGAAAAT CAAATTCCTT AGCAAAAGCG TCAAGTTGCT TCATGAAAGC 
TATAACTCAT TTACGCCAAC GTACGCAGTA ACGGATTTCG TGAAGGAGTA TGCCAAGCTT 
CTGGAGCTTA AGAAGAATTT ACTTCGACAG GCGAACGCAA CCACGACAAG GCAGATTGAT 
GAAGACTACG AGAGAGTATT CGCAAAACTA AAGGATCAAA TTGATGGCAG TCAAATACAG 
GATAAGATAA ATCTCATCAA GTTCGAAAGT CAATGCGAGT ATCTCACTAA ACAACTAGTC 
CTGTCTCAAG AACAGATCGA GCAGTTGCAG GAGGAAAACT CTGCTTTGAA ACTGGCCTCC 
GTTGAAACTA CAAGGCATTG GGATACCATC GAGCTGATCT TCAACGGAAA ACCAAAGCCA 
ACCAAACCGG TAGACAAATT TTTCGACAAG GAAGTTCAGG TGACCGTTGA AATCAGTCAC 
AAATGTATCA ACACAATTCC GATCATCGAC GACAGCCTAG CGGAGAAACC TCGTCAGGCT 
ACTCCACCTG CGACGACGCA TTCCTCTGAG CATTCGTCAG TGAGGAAAAC CTCAGATGCT 
GGACAGATGA CAGACGACTT GTACGAAGCT CAACCATCGA CATCCGTTCC GGTGGCACTG 
CAAAAGTCCC TGGAATCACA GCTGAAGCAA GCCATGATGT TGGCTTCAAC TCGCAGCGCT 
TTGCTTCTGG AGACGGAAAG TCGGCTAACG GAGTGCCACG GACGAATCAA GCTGTTAGAA 
AGGTCTTTGG AGGATAAGGA AGAACAGCTG AGAAGACAGG CACATGCTGC GGCAAACAAC 
GAGAAGCAAG ACGATAACAT TCTTAGTTCG ACGATTGGAT CCCTACAAAA TCTTCTACTA 
GAAAAAGACA CCACTCTTTC GCGGTATCAG GAGCTGCTTA AAAACGAACG TCAAGATCAT 
TCAAAAACCT ACGACGAGAA CATTGCGCAG ATCAGGGCTC TGAAGAAGAC TGTTGACGAA 
ATGGAACAGA AAGTGTACGA CAAGCAGAAG GAAATCGACA ATTTAAGCAT TCAGCTAACT 
GATTTGACTC AGCTCAAAGC ACAACATGAA GCAGCTGCTG CAGTCCGGGA AGCTCAACAG 
GCGGAGTCTA GGCAAGCCAA CAAGCCAGAA TTGATATACA CCGACAAGCT CATCGAGAAC 
ATTTTTGAGA CCGATCAGAA AAACGAGGAG GAGATTGAGG AGCTGAAAAG GAGAATCAAA 
GAGCTGGAAA GCAAGCTTCA AAGTGCAAGT GAAGAACTGC GGAAAGGCCA AACGAATCTG 
AGGGAGATGA TCGCGAGAGA GAAGCGCGCC GAGAAGAATT TGAGAGAAAA GGAAGCGGAA 
ATTGTGGCGC TGAACGAGCG ACTTGCCAGA GAACATGAGG ATTTGAGAGA ATTCACCGAC 
AACATAGCGA GCGCTCAGGA GATTGAACAA CTGAAGGAAA TGCTGGAGGA AAAGGACCGT 
CACATACAGG ATCTGACCGA GACTTTGAGT CAGTTTCACG ACGATCAACA AAAGTTTATG 
AACGACACTT CCCTACATTC TGCTGAACAA GTTTCTCAGC TTAGCGCAGA TTTGAATCGA 
AGCGAGGCTT CGAATCGTGT GCTCAAAACG CAAATTGAAG CACTGAAGCG TCAGATATTG 
AGCATCCAAC AACGTGAGAA GCAATCACGT GATTTGGTAA AAACTCTAAA GAACCAGCTC 
ATAAAACGAC CGGTTATTTC GATGAAACCG GATCGAACTC CAACTCCAAG AGAAGAGCAA 
CTTGCCCGGA AACTGCAACA ACTGGAGACC GAGCTGTTGG ACACGAAGGA TGAACTGCGC 
AAGCAGATCA ACATCAATGA GAATCGTCGC GCGAAGAATG CGGCCGAGTT GGATTTGTGG 
AACAAACAGA AGCGTTGGCA GCAGATGGCC GAAAAGCTGA AGGTACAGCT CAAGGAGCGT 
GAAGTTGAGT TGGATAAGTT GAAGGTTCAT TTCAATTCAG CCAAAAATAC AATAGTACGA 
CTAGAACGGG AGAAGGCCAT TTTGGAGGGA AGAAGCATTC GTGGCAGCAG TGCGGGATCT 
GTTACGTTGG CCGCTTCACG GTTTGGTGCT GGGGATAGCA AGTACACTCC GGCAGATTCT 
CCGGATTCGT GCACCACAGA AGGTACGGCG TCGGACGAGG CTAGCACTGA AGTGAACACC 
TTTGCGCAAA ATAGCAAGGA GATTATCGAG GCACTGAAGA ACAGAATTGA GTCGCAACAG 
CGAAGGATTG TGGCAATGGA GTTGGAGCGA AAGGGCTCCA ACGCGATGAC CCACGAGCTG 
GAACGGATGC AGGAAAAGCA ATCCAATCTA GAGGCACAAA ACATTCGACT GGAAGCGAAA 
GCCCTTCAGC TTCAGCTGGA CAATGACATG CTTCGTCAGA GTGACGAGTC TGAAAGACTG 
CGCAGCCAGA TTAAGCATCT TGAAGAGTAC ATAATAGTGT TGAAGGAGGA ACTTAGCAAG 
GTAACAGCGG GTTGTCCGGA AACGATAAAC TTTGATAACT TGTGTCAGCG ATGCTCCCGA 
TGTAGTACCT CCGGATCGGC CACCAATAGC GAGCTGGCCG ATTACAACTC CAAATTGGAG 
CAAACTGTCA TTGCGCTACG CAGGGTTGTG GAAAAACTAA AAGTGGAGAA CAAGCAACTC 
AAGGAGAACA AACCATGTCC GATGACAACG GCAAACGATC GCAAATCTGC CCAGGGGATG 
GTGAGCAAAG AAAGTTACGA ACGTCTGCAG AAGGAACATG ACAAGCTTCA GCAAAACTAC 
ACGGAAGCCT TGAATCGTGT GGCTGCCCTA CAGGTGGAAG TTGAACTGCT ATCCAGTGTG 
ACCTGCCCAC GGTGCCATCC GCGGGAGGGT GCTTCCCAGG AGTCGGAACC GGACTTGTTG 
GAAAAGAAAA CGCAACTGCT GGAGAAGGCG AAAATTCTGT TGAGCCGTGC AGCCGCTAAG 
GAACGTTACT TGAAGGAACA AATTGCGCTG CTCAGAAGGA AATGTTCAGA TTTGCAAAAC 
GTACCTGTTA TCGATGAGAT CAGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021697462.1
CDS	125..6394
Translation	MDWRYMLSVSPHNLADERKDEVAVHLAAVPTDQKLDSKSCKKLFRLAQVVLRFRNEQVGQLRIEIDRMVEENEQRDARGSPSDQLREQVADLKRIIDKLEAERHTSRARIKELSEEMTSLQRKLAESSHFGSEGEDSPDALSEIDRQQELYNNISMKNKHIKRLLRDLDDLEKRNSFQIDTINSLQVSLNDATMNLTGLTHQYEEAQAIMKEQEVLIEESNEKIKRLEEDVEGLAEEKVRFEEDLEGFAKQLEERTEQWQSILEKKQEELEEIETKYSELLQQFPGYDIEAERKEFKKMSARLQEKDEMIEELEKKITMLSQEILTSTELMNRISQEKENASKDTVKVSQCCEESRALLEKANKRCEEMQEILSNVEEDNMLKSKQAVEAIEALRRYESGEEGLANALKKINRLHEKVNVRDKQIRELVAEINLANEIAMENAVLRKRLGISEDEVIPTHSVLSKQRKIEKVNERLALKLRASEEMRLQLKLEKNDLKRKLFQMSQSLSGQRSLDKEKAMISINSNHR*SAVTEGNQATDAVDEISKLSDTDVAKFCEKCMKQYNVNESRKFCKACIFRQSFNYCDGCIKKLKGNINSDEDPKTKYAEKIEELEKKYAVVVEENENLRIGMHEILQKLREYDATSNHLTIDTSTLERLLHALDARSVSGWYHPAMRLQNELLATREREMLLKERLSLKSKQSTFSLGSRNDSGNVCDDEENTADIDQVITEEPDFGEQVLLKSVEIDRLNDQIESLKEEREQLLQSNDELVVTRKLYNELMEFTKSTDNEKDRILVETLERLKLIESNIYAFQRKIEFLKAENDNMHNTMRTIEVEHLNILHGLRSELARKNSTLKKLEHQTSVEGRNVSSPDSESIGKLESEIERMKLETANFYTIFLRNIQEVDKDNLLHLEYENLARVGLVESNLAVDFMPKEEFKRIKKQLQTAQEEFKKQTIKNGHLEELLKISQEQLRSQQMLISKFSDEEISLRHLVADLQSSSNEKYLLVKTQKELDAAKEQGELFKLSNAKLKQSLIDSGEELNKLKEQLAQQELDFIEHQKDNELKIKFLSKSVKLLHESYNSFTPTYAVTDFVKEYAKLLELKKNLLRQANATTTRQIDEDYERVFAKLKDQIDGSQIQDKINLIKFESQCEYLTKQLVLSQEQIEQLQEENSALKLASVETTRHWDTIELIFNGKPKPTKPVDKFFDKEVQVTVEISHKCINTIPIIDDSLAEKPRQATPPATTHSSEHSSVRKTSDAGQMTDDLYEAQPSTSVPVALQKSLESQLKQAMMLASTRSALLLETESRLTECHGRIKLLERSLEDKEEQLRRQAHAAANNEKQDDNILSSTIGSLQNLLLEKDTTLSRYQELLKNERQDHSKTYDENIAQIRALKKTVDEMEQKVYDKQKEIDNLSIQLTDLTQLKAQHEAAAAVREAQQAESRQANKPELIYTDKLIENIFETDQKNEEEIEELKRRIKELESKLQSASEELRKGQTNLREMIAREKRAEKNLREKEAEIVALNERLAREHEDLREFTDNIASAQEIEQLKEMLEEKDRHIQDLTETLSQFHDDQQKFMNDTSLHSAEQVSQLSADLNRSEASNRVLKTQIEALKRQILSIQQREKQSRDLVKTLKNQLIKRPVISMKPDRTPTPREEQLARKLQQLETELLDTKDELRKQININENRRAKNAAELDLWNKQKRWQQMAEKLKVQLKEREVELDKLKVHFNSAKNTIVRLEREKAILEGRSIRGSSAGSVTLAASRFGAGDSKYTPADSPDSCTTEGTASDEASTEVNTFAQNSKEIIEALKNRIESQQRRIVAMELERKGSNAMTHELERMQEKQSNLEAQNIRLEAKALQLQLDNDMLRQSDESERLRSQIKHLEEYIIVLKEELSKVTAGCPETINFDNLCQRCSRCSTSGSATNSELADYNSKLEQTVIALRRVVEKLKVENKQLKENKPCPMTTANDRKSAQGMVSKESYERLQKEHDKLQQNYTEALNRVAALQVEVELLSSVTCPRCHPREGASQESEPDLLEKKTQLLEKAKILLSRAAAKERYLKEQIALLRRKCSDLQNVPVIDEISE

Target ORF information:

RefSeq Version	XM_021841770.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti centrosomal protein of 290 kDa (LOC5567088), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021841770.1

ORF Insert Sequence:

ATGGATTGGC GCTACATGCT GTCCGTGTCG CCGCACAATC TGGCCGACGA GCGCAAGGAT 
GAAGTGGCCG TCCATTTGGC TGCCGTTCCA ACCGACCAGA AGTTGGACAG CAAGAGCTGT 
AAAAAGTTGT TCCGGTTGGC GCAGGTGGTG CTACGCTTTC GCAACGAGCA GGTTGGTCAA 
CTTAGAATCG AAATCGACCG AATGGTGGAA GAGAACGAAC AGCGCGATGC ACGAGGATCA 
CCCTCAGATC AGCTCCGGGA GCAAGTGGCA GACCTCAAGC GAATCATTGA CAAGCTGGAA 
GCAGAACGGC ACACGAGCCG TGCTAGGATC AAGGAGCTAT CTGAAGAAAT GACCTCACTA 
CAGAGAAAGC TAGCGGAAAG CAGTCACTTC GGCAGTGAAG GTGAAGACTC ACCAGACGCG 
CTGTCGGAAA TCGATCGCCA ACAGGAACTG TACAACAACA TCAGTATGAA GAATAAACAC 
ATTAAGCGTT TGCTGCGAGA CTTAGATGAT TTGGAAAAAC GTAACAGCTT CCAGATCGAC 
ACCATCAACA GTTTGCAGGT CAGCCTGAAC GATGCAACAA TGAATTTGAC CGGATTGACT 
CATCAATATG AGGAAGCCCA AGCGATTATG AAGGAGCAGG AAGTTCTGAT TGAAGAATCT 
AATGAGAAAA TCAAACGATT GGAAGAGGAT GTGGAGGGGT TAGCCGAAGA GAAAGTTAGA 
TTTGAGGAAG ATTTGGAAGG GTTTGCGAAG CAGCTAGAAG AACGGACTGA GCAGTGGCAA 
AGTATCCTAG AGAAAAAGCA AGAAGAGTTG GAGGAAATCG AAACAAAATA TTCAGAGTTG 
CTGCAACAGT TTCCTGGGTA TGACATCGAA GCGGAGAGAA AGGAATTCAA AAAGATGAGC 
GCAAGGCTTC AGGAAAAAGA TGAGATGATT GAAGAGTTGG AGAAGAAAAT CACTATGCTA 
TCGCAGGAGA TTCTAACGTC CACGGAGCTT ATGAACAGGA TTTCGCAAGA GAAAGAGAAT 
GCCAGTAAGG ATACGGTGAA GGTAAGTCAG TGTTGCGAGG AAAGCCGTGC GTTGCTGGAG 
AAGGCAAATA AGCGATGCGA AGAGATGCAG GAAATTTTGT CCAATGTGGA GGAGGATAAC 
ATGTTGAAGT CGAAACAGGC GGTCGAAGCG ATCGAGGCAT TGAGAAGATA CGAAAGTGGC 
GAGGAAGGTT TGGCGAATGC ATTGAAGAAG ATCAACAGGT TGCATGAGAA AGTTAACGTA 
CGGGATAAGC AGATACGGGA ATTGGTGGCG GAAATCAATC TGGCGAACGA GATTGCCATG 
GAGAATGCTG TGCTGAGGAA GCGTTTGGGT ATTAGTGAGG ACGAAGTTAT ACCAACTCAT 
TCTGTTCTGT CTAAGCAGAG GAAAATTGAG AAAGTAAACG AACGCTTGGC TTTGAAATTA 
AGAGCATCGG AGGAAATGCG ACTTCAACTG AAGTTGGAGA AGAATGATCT GAAGCGAAAA 
CTTTTTCAGA TGTCACAATC TTTGAGTGGT CAAAGGAGTT TAGACAAGGA AAAAGCGATG 
ATATCAATCA ACAGCAATCA TCGGTGAAGC GCCGTAACGG AAGGAAATCA GGCAACAGAT 
GCAGTCGATG AAATCTCGAA GCTTAGTGAT ACGGATGTTG CGAAGTTCTG TGAGAAATGC 
ATGAAGCAGT ATAACGTTAA TGAGAGTAGA AAGTTTTGCA AGGCGTGCAT CTTTCGGCAG 
AGCTTCAACT ATTGTGATGG ATGCATCAAA AAATTGAAGG GTAATATCAA CTCGGACGAA 
GATCCAAAGA CAAAGTATGC TGAAAAGATT GAAGAATTGG AGAAAAAGTA CGCTGTTGTT 
GTGGAGGAAA ATGAGAACTT GAGAATAGGG ATGCATGAGA TTTTGCAGAA ATTGCGAGAG 
TACGATGCTA CGTCAAATCA TTTAACGATC GACACATCTA CTTTAGAACG ATTGTTGCAT 
GCACTGGATG CCAGAAGCGT GAGTGGATGG TATCACCCGG CTATGCGGTT ACAGAACGAA 
TTGCTGGCCA CGAGAGAAAG GGAAATGCTG CTAAAAGAAC GTTTGAGTCT CAAAAGCAAA 
CAAAGCACAT TTTCACTGGG AAGTCGAAAT GATTCCGGCA ATGTTTGTGA TGATGAGGAA 
AACACAGCGG ATATAGATCA AGTTATAACA GAGGAGCCGG ATTTTGGGGA GCAAGTGCTG 
CTCAAATCGG TGGAAATCGA TCGACTGAAT GATCAAATCG AATCTCTGAA GGAAGAACGA 
GAGCAATTGC TTCAATCAAA TGACGAACTA GTAGTGACGA GAAAGTTGTA TAATGAATTG 
ATGGAATTTA CCAAATCGAC CGATAATGAG AAAGATAGGA TATTGGTTGA AACGCTAGAG 
CGGCTGAAGC TGATAGAATC CAATATATAT GCCTTCCAGA GAAAGATTGA GTTCTTGAAA 
GCCGAGAATG ACAACATGCA CAACACAATG CGGACAATCG AAGTAGAGCA CTTGAACATT 
CTCCACGGGT TGAGATCCGA ATTGGCGCGG AAGAATAGCA CATTGAAAAA GTTGGAACAT 
CAAACCAGCG TTGAGGGAAG GAATGTAAGT AGTCCGGATT CAGAATCGAT CGGGAAGCTA 
GAGTCTGAGA TTGAGCGAAT GAAGCTGGAG ACGGCCAATT TCTATACGAT CTTCTTGAGA 
AACATTCAGG AAGTGGACAA GGACAATCTG TTGCATTTGG AGTATGAGAA TCTAGCTCGC 
GTTGGGTTGG TTGAATCGAA TTTGGCGGTA GATTTTATGC CGAAAGAAGA GTTTAAGAGG 
ATCAAGAAAC AGTTACAGAC AGCACAGGAA GAGTTCAAAA AACAAACTAT TAAGAATGGT 
CACTTGGAAG AACTTCTGAA GATATCACAA GAACAACTTC GGTCACAGCA AATGTTGATT 
TCAAAGTTTT CAGATGAGGA AATCAGTTTG CGGCATTTGG TTGCGGATTT ACAGAGTTCA 
TCAAACGAAA AGTATTTACT GGTCAAAACT CAGAAGGAGC TTGACGCTGC CAAAGAGCAA 
GGAGAGCTTT TTAAATTAAG TAATGCTAAG CTGAAGCAAT CGTTGATTGA CAGCGGCGAG 
GAGTTGAATA AGTTAAAGGA GCAGCTAGCT CAACAAGAGT TAGATTTCAT CGAACATCAG 
AAAGACAACG AGCTGAAAAT CAAATTCCTT AGCAAAAGCG TCAAGTTGCT TCATGAAAGC 
TATAACTCAT TTACGCCAAC GTACGCAGTA ACGGATTTCG TGAAGGAGTA TGCCAAGCTT 
CTGGAGCTTA AGAAGAATTT ACTTCGACAG GCGAACGCAA CCACGACAAG GCAGATTGAT 
GAAGACTACG AGAGAGTATT CGCAAAACTA AAGGATCAAA TTGATGGCAG TCAAATACAG 
GATAAGATAA ATCTCATCAA GTTCGAAAGT CAATGCGAGT ATCTCACTAA ACAACTAGTC 
CTGTCTCAAG AACAGATCGA GCAGTTGCAG GAGGAAAACT CTGCTTTGAA ACTGGCCTCC 
GTTGAAACTA CAAGGCATTG GGATACCATC GAGCTGATCT TCAACGGAAA ACCAAAGCCA 
ACCAAACCGG TAGACAAATT TTTCGACAAG GAAGTTCAGG TGACCGTTGA AATCAGTCAC 
AAATGTATCA ACACAATTCC GATCATCGAC GACAGCCTAG CGGAGAAACC TCGTCAGGCT 
ACTCCACCTG CGACGACGCA TTCCTCTGAG CATTCGTCAG TGAGGAAAAC CTCAGATGCT 
GGACAGATGA CAGACGACTT GTACGAAGCT CAACCATCGA CATCCGTTCC GGTGGCACTG 
CAAAAGTCCC TGGAATCACA GCTGAAGCAA GCCATGATGT TGGCTTCAAC TCGCAGCGCT 
TTGCTTCTGG AGACGGAAAG TCGGCTAACG GAGTGCCACG GACGAATCAA GCTGTTAGAA 
AGGTCTTTGG AGGATAAGGA AGAACAGCTG AGAAGACAGG CACATGCTGC GGCAAACAAC 
GAGAAGCAAG ACGATAACAT TCTTAGTTCG ACGATTGGAT CCCTACAAAA TCTTCTACTA 
GAAAAAGACA CCACTCTTTC GCGGTATCAG GAGCTGCTTA AAAACGAACG TCAAGATCAT 
TCAAAAACCT ACGACGAGAA CATTGCGCAG ATCAGGGCTC TGAAGAAGAC TGTTGACGAA 
ATGGAACAGA AAGTGTACGA CAAGCAGAAG GAAATCGACA ATTTAAGCAT TCAGCTAACT 
GATTTGACTC AGCTCAAAGC ACAACATGAA GCAGCTGCTG CAGTCCGGGA AGCTCAACAG 
GCGGAGTCTA GGCAAGCCAA CAAGCCAGAA TTGATATACA CCGACAAGCT CATCGAGAAC 
ATTTTTGAGA CCGATCAGAA AAACGAGGAG GAGATTGAGG AGCTGAAAAG GAGAATCAAA 
GAGCTGGAAA GCAAGCTTCA AAGTGCAAGT GAAGAACTGC GGAAAGGCCA AACGAATCTG 
AGGGAGATGA TCGCGAGAGA GAAGCGCGCC GAGAAGAATT TGAGAGAAAA GGAAGCGGAA 
ATTGTGGCGC TGAACGAGCG ACTTGCCAGA GAACATGAGG ATTTGAGAGA ATTCACCGAC 
AACATAGCGA GCGCTCAGGA GATTGAACAA CTGAAGGAAA TGCTGGAGGA AAAGGACCGT 
CACATACAGG ATCTGACCGA GACTTTGAGT CAGTTTCACG ACGATCAACA AAAGTTTATG 
AACGACACTT CCCTACATTC TGCTGAACAA GTTTCTCAGC TTAGCGCAGA TTTGAATCGA 
AGCGAGGCTT CGAATCGTGT GCTCAAAACG CAAATTGAAG CACTGAAGCG TCAGATATTG 
AGCATCCAAC AACGTGAGAA GCAATCACGT GATTTGGTAA AAACTCTAAA GAACCAGCTC 
ATAAAACGAC CGGTTATTTC GATGAAACCG GATCGAACTC CAACTCCAAG AGAAGAGCAA 
CTTGCCCGGA AACTGCAACA ACTGGAGACC GAGCTGTTGG ACACGAAGGA TGAACTGCGC 
AAGCAGATCA ACATCAATGA GAATCGTCGC GCGAAGAATG CGGCCGAGTT GGATTTGTGG 
AACAAACAGA AGCGTTGGCA GCAGATGGCC GAAAAGCTGA AGGTACAGCT CAAGGAGCGT 
GAAGTTGAGT TGGATAAGTT GAAGGTTCAT TTCAATTCAG CCAAAAATAC AATAGTACGA 
CTAGAACGGG AGAAGGCCAT TTTGGAGGGA AGAAGCATTC GTGGCAGCAG TGCGGGATCT 
GTTACGTTGG CCGCTTCACG GTTTGGTGCT GGGGATAGCA AGTACACTCC GGCAGATTCT 
CCGGATTCGT GCACCACAGA AGGTACGGCG TCGGACGAGG CTAGCACTGA AGTGAACACC 
TTTGCGCAAA ATAGCAAGGA GATTATCGAG GCACTGAAGA ACAGAATTGA GTCGCAACAG 
CGAAGGATTG TGGCAATGGA GTTGGAGCGA AAGGGCTCCA ACGCGATGAC CCACGAGCTG 
GAACGGATGC AGGAAAAGCA ATCCAATCTA GAGGCACAAA ACATTCGACT GGAAGCGAAA 
GCCCTTCAGC TTCAGCTGGA CAATGACATG CTTCGTCAGA GTGACGAGTC TGAAAGACTG 
CGCAGCCAGA TTAAGCATCT TGAAGAGTAC ATAATAGTGT TGAAGGAGGA ACTTAGCAAG 
GTAACAGCGG GTTGTCCGGA AACGATAAAC TTTGATAACT TGTGTCAGCG ATGCTCCCGA 
TGTAGTACCT CCGGATCGGC CACCAATAGC GAGCTGGCCG ATTACAACTC CAAATTGGAG 
CAAACTGTCA TTGCGCTACG CAGGGTTGTG GAAAAACTAA AAGTGGAGAA CAAGCAACTC 
AAGGAGAACA AACCATGTCC GATGACAACG GCAAACGATC GCAAATCTGC CCAGGGGATG 
GTGAGCAAAG AAAGTTACGA ACGTCTGCAG AAGGAACATG ACAAGCTTCA GCAAAACTAC 
ACGGAAGCCT TGAATCGTGT GGCTGCCCTA CAGGTGGAAG TTGAACTGCT ATCCAGTGTG 
ACCTGCCCAC GGTGCCATCC GCGGGAGGGT GCTTCCCAGG AGTCGGAACC GGACTTGTTG 
GAAAAGAAAA CGCAACTGCT GGAGAAGGCG AAAATTCTGT TGAGCCGTGC AGCCGCTAAG 
GAACGTTACT TGAAGGAACA AATTGCGCTG CTCAGAAGGA AATGTTCAGA TTTGCAAAAC 
GTACCTGTTA TCGATGAGAT CAGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL005809 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

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Target ORF information:

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