LOC5566304 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5566304
Entrez Gene ID	5566304
Full Name	UPF0518 protein AAEL005291
Synonyms	AAEL005291,AAEL010515,U518_AEDAE
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001650633.2	XP_001650683.2	UPF0518 protein AAEL005291 isoform X2
XM_021853491.1	XP_021709183.1	UPF0518 protein AAEL005291 isoform X1
XM_021853494.1	XP_021709186.1	UPF0518 protein AAEL005291 isoform X3
XM_001650633.1	XP_001650683.1	AAEL005291-PA
XM_021853492.1	XP_021709184.1	UPF0518 protein AAEL005291 isoform X1
XM_021853493.1	XP_021709185.1	UPF0518 protein AAEL005291 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5566304 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5566304 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe02418	XM_001650633.1 Latest version!	Aedes aegypti AAEL005291-RA (U518_AEDAE), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe128627	XM_021853491.1 Latest version!	Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe128627	XM_021853493.1 Latest version!	Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe128627	XM_021853492.1 Latest version!	Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe128628	XM_001650633.2 Latest version!	Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe128629	XM_021853494.1 Latest version!	Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5566304 ( XM_001650633.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001650683.1 Aedes aegypti AAEL005291-RA (U518_AEDAE), partial mRNA.

Price & Availability↑

CloneID	OAe02418
Clone ID Related Accession (Same CDS sequence)	XM_001650633.1
Accession Version	XM_001650633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL005291-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810351). COMPLETENESS: incomplete on both ends.

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTTACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAATGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCAGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCTG TCCCAGAAGA 
CTCGATCGTG GGACGTACAT TGCAACATAC TCATCGATCT GTCCGGTGTG GTTCACCCGG 
GCTGGGGAAG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATTGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAA CTGCAGGAGC TGACGCTGTT TATGAACTCG 
CTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCA GTGTTGGGTC CGGCCGTTCT CCAGACAAAC 
GTCGAGTCGC AGATTTCGGC GATGGCCTAT CTGGACCTGA TCGTACGCTC GGTAACCGAA 
CCGGGCCTGA TTCAGATCGT GGTCAAGTTT CTACTGGACG AGGAGAAGTT CGACGGTCAG 
CGGATATTGG ACGTCCTGGT GGAGCGGCTC AACAGCAACG ACACAAGGCT TTGCATGGTA 
TCCTTATCGC TGTTCGACAC ACTTCTGAGT CTCAACTGCG AGGACATCAT GCTGGAACTG 
GCCCTGAAGT ACCTGCTCAA CTGTCAACAC GTGCCTATCT CGCATCGGTT CAAGGTGAAC 
CGGGTAGATC CATACAGCAA CGCCGTCGAG TACTTTCTAA ACACATCTCC GGACATCATG 
AAAAAAGTGA ACAACGTGTT GAACATCAAC AACAACAATA ACAACACCAG TCAAGCGTCG 
TCGATGCTGG GGAGTGGCCC CTCATCAATG CCCCAGGGCG GACGTAATGT TAGCAAAACG 
ATCGGTGCCA ACTGGAACCA TTACGGGAAC AATACCGGAG AAACGCTTAT GGCTAACTAC 
CAAGCGTACC TGTTGGAGGC CCGTAACCGG ATAGTTCAAT GCAAACATGC CTGTGATCAG 
TGGAACAATG TGTATCGTTA CCAAAAATTG AGCAAACTGG TAAACAATAG TAGTAGTTTA 
AGTCATAGCG GTAATGGAAC GGCTGGGAGC GAAGACGTCC GAACTTACAA AGTACAAATG 
ATCAAAAATT TCCTGGCGGA ATTTACCACC GCTCCGGACA GTGCGGTCGA CATGGCGGGA 
GATGGTGACC GATCGAGTCT GTGCCAGTCA CCGGCGGGGA TGTTCGGTAC CGGGCATCAT 
GCGTCCCACC TGATGGCGAC AACGTCGAAG CAGTTGGACA GTTTGCAATC GCTGGGCGAT 
AGCAGCGGGT ACGAAAGTTT GAACATTACC AATTTGGGGA CGGGCAGTGA AGATGGTCGG 
CGACACGAAG CGTGGAAGGT TTCCAGCGTG AAGGAAGAAA CCATTGTGGA TTTGGATCTG 
TCGGAGGATC TGTTCGCGCA GGGGACCGTA AGTTTAGGTC CCTTCCTAAC GGCCATCTGG 
GGCAAGTTGC AAACGTTTAC GAGCAATTGC TTGTACGTCA ACCTTCACCT GACCGGACTG 
ATAAGCCATC TGGCGTGGTT TCCTCTGCCG TTGCTGCACT CGATTCTGCT GCGACCCGAC 
ATACCGACCA CATCCGATAC GCCGTCGTTT CATCAGGTCC TGAAAATTCT CAAACAACAA 
ATCGACGCCG AGCTGCCCGA CTGCGACGAG TCGCTAGAAA TCGTGGACGT TGCCCGCTCC 
TTTCTCGTCG ACCGAGAATT CCGACTCATC AATATGCGCA AGAATGCCAT CGAATCGAAC 
CCACACAGTG GCAAACTGTT GCTCCCGAAT GGTGCAACCG GAGCATCCGG ACCCGTATCG 
ATGACCTCCA ACCTCTCGCA GACGACACCG ATGCAGCTCA CTCCGAGCTC CTCGTACGAT 
CCGTTCAAAC GGAACGATAC CAAACGGAAA AGCATTTCCA ATTCGTTTTC CAGTATTTTC 
CGACGACCAG GATCCTCCGC TTCATCTGGT CTAGCACAAG TATTTCAATT TTTTACCGGA 
GGAGGGTCCA GCAGCAACAG CTCGTCGATC AGCCATCAAT CTTCCTCGAC GCCATCGCCA 
GCCGGATCCC AGCAGTATCT TTCGTCCAAC AGCTCCGGGG TAAGTAGCTT CATGGGAAGC 
AGTCGGCGGG AATCGCGTGA AGCGGAAACC CAATTCGTGG ATCATCCGTC AATTGGGGGA 
CCACCGAGCA GTATGGGTCC CACTAGTCTA ACGTCGACCG GGAGTCCCCA CAACAGCCTC 
GAGTACTCGC TGGTCAATAT CAACGGTTCC ATTGTCGGAG GGATTGGAAG CGAAAGGCAG 
CGGGATCTGG CCGTCAGTGC CGTTGTTTTG GACGAGTGGT TGAAGGAACT TGCGGCCATC 
ACTCAGGAGC AGTGCATTAT AATGATATCG GACCAGGTGT CATCCCAAAA GCCGGGGAAA 
CTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001650683.1
CDS	1..3129
Translation	MSWLRSSPLRQSFSKAGGGSGSGASSRGGNSTNGTVRAFDASECDPKACYDSFCIHWQQAFEIIQRSENNRGQSHDDVLGVVTHLDHMVTLLLVELHHCNKVGLPGAPAPPAPCLEHLLSENLLDKLYEWGVKTGRYANAVRLEQLKLYEQLVSHSRHQLLVHEPFLRPLLKILASSQNEIYPPDVEKRLVILLNQLCVVLMQNVHLLDLFFFSTAQQNGSGGHANFIIFSLLIPYVHQEGSLGHQARDALLLCMACPRRLDRGTYIATYSSICPVWFTRAGEGLYSRLPNQIDIKTIDWYRITTDDVTELQELTLFMNSLEFCNAVVQVAHSMIRQQLLDFLYQGFLVPVLGPAVLQTNVESQISAMAYLDLIVRSVTEPGLIQIVVKFLLDEEKFDGQRILDVLVERLNSNDTRLCMVSLSLFDTLLSLNCEDIMLELALKYLLNCQHVPISHRFKVNRVDPYSNAVEYFLNTSPDIMKKVNNVLNINNNNNNTSQASSMLGSGPSSMPQGGRNVSKTIGANWNHYGNNTGETLMANYQAYLLEARNRIVQCKHACDQWNNVYRYQKLSKLVNNSSSLSHSGNGTAGSEDVRTYKVQMIKNFLAEFTTAPDSAVDMAGDGDRSSLCQSPAGMFGTGHHASHLMATTSKQLDSLQSLGDSSGYESLNITNLGTGSEDGRRHEAWKVSSVKEETIVDLDLSEDLFAQGTVSLGPFLTAIWGKLQTFTSNCLYVNLHLTGLISHLAWFPLPLLHSILLRPDIPTTSDTPSFHQVLKILKQQIDAELPDCDESLEIVDVARSFLVDREFRLINMRKNAIESNPHSGKLLLPNGATGASGPVSMTSNLSQTTPMQLTPSSSYDPFKRNDTKRKSISNSFSSIFRRPGSSASSGLAQVFQFFTGGGSSSNSSSISHQSSSTPSPAGSQQYLSSNSSGVSSFMGSSRRESREAETQFVDHPSIGGPPSSMGPTSLTSTGSPHNSLEYSLVNINGSIVGGIGSERQRDLAVSAVVLDEWLKELAAITQEQCIIMISDQVSSQKPGKLS

Target ORF information:

RefSeq Version	XM_001650633.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL005291-RA (U518_AEDAE), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001650633.1

ORF Insert Sequence:

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTTACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAATGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCAGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCTG TCCCAGAAGA 
CTCGATCGTG GGACGTACAT TGCAACATAC TCATCGATCT GTCCGGTGTG GTTCACCCGG 
GCTGGGGAAG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATTGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAA CTGCAGGAGC TGACGCTGTT TATGAACTCG 
CTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCA GTGTTGGGTC CGGCCGTTCT CCAGACAAAC 
GTCGAGTCGC AGATTTCGGC GATGGCCTAT CTGGACCTGA TCGTACGCTC GGTAACCGAA 
CCGGGCCTGA TTCAGATCGT GGTCAAGTTT CTACTGGACG AGGAGAAGTT CGACGGTCAG 
CGGATATTGG ACGTCCTGGT GGAGCGGCTC AACAGCAACG ACACAAGGCT TTGCATGGTA 
TCCTTATCGC TGTTCGACAC ACTTCTGAGT CTCAACTGCG AGGACATCAT GCTGGAACTG 
GCCCTGAAGT ACCTGCTCAA CTGTCAACAC GTGCCTATCT CGCATCGGTT CAAGGTGAAC 
CGGGTAGATC CATACAGCAA CGCCGTCGAG TACTTTCTAA ACACATCTCC GGACATCATG 
AAAAAAGTGA ACAACGTGTT GAACATCAAC AACAACAATA ACAACACCAG TCAAGCGTCG 
TCGATGCTGG GGAGTGGCCC CTCATCAATG CCCCAGGGCG GACGTAATGT TAGCAAAACG 
ATCGGTGCCA ACTGGAACCA TTACGGGAAC AATACCGGAG AAACGCTTAT GGCTAACTAC 
CAAGCGTACC TGTTGGAGGC CCGTAACCGG ATAGTTCAAT GCAAACATGC CTGTGATCAG 
TGGAACAATG TGTATCGTTA CCAAAAATTG AGCAAACTGG TAAACAATAG TAGTAGTTTA 
AGTCATAGCG GTAATGGAAC GGCTGGGAGC GAAGACGTCC GAACTTACAA AGTACAAATG 
ATCAAAAATT TCCTGGCGGA ATTTACCACC GCTCCGGACA GTGCGGTCGA CATGGCGGGA 
GATGGTGACC GATCGAGTCT GTGCCAGTCA CCGGCGGGGA TGTTCGGTAC CGGGCATCAT 
GCGTCCCACC TGATGGCGAC AACGTCGAAG CAGTTGGACA GTTTGCAATC GCTGGGCGAT 
AGCAGCGGGT ACGAAAGTTT GAACATTACC AATTTGGGGA CGGGCAGTGA AGATGGTCGG 
CGACACGAAG CGTGGAAGGT TTCCAGCGTG AAGGAAGAAA CCATTGTGGA TTTGGATCTG 
TCGGAGGATC TGTTCGCGCA GGGGACCGTA AGTTTAGGTC CCTTCCTAAC GGCCATCTGG 
GGCAAGTTGC AAACGTTTAC GAGCAATTGC TTGTACGTCA ACCTTCACCT GACCGGACTG 
ATAAGCCATC TGGCGTGGTT TCCTCTGCCG TTGCTGCACT CGATTCTGCT GCGACCCGAC 
ATACCGACCA CATCCGATAC GCCGTCGTTT CATCAGGTCC TGAAAATTCT CAAACAACAA 
ATCGACGCCG AGCTGCCCGA CTGCGACGAG TCGCTAGAAA TCGTGGACGT TGCCCGCTCC 
TTTCTCGTCG ACCGAGAATT CCGACTCATC AATATGCGCA AGAATGCCAT CGAATCGAAC 
CCACACAGTG GCAAACTGTT GCTCCCGAAT GGTGCAACCG GAGCATCCGG ACCCGTATCG 
ATGACCTCCA ACCTCTCGCA GACGACACCG ATGCAGCTCA CTCCGAGCTC CTCGTACGAT 
CCGTTCAAAC GGAACGATAC CAAACGGAAA AGCATTTCCA ATTCGTTTTC CAGTATTTTC 
CGACGACCAG GATCCTCCGC TTCATCTGGT CTAGCACAAG TATTTCAATT TTTTACCGGA 
GGAGGGTCCA GCAGCAACAG CTCGTCGATC AGCCATCAAT CTTCCTCGAC GCCATCGCCA 
GCCGGATCCC AGCAGTATCT TTCGTCCAAC AGCTCCGGGG TAAGTAGCTT CATGGGAAGC 
AGTCGGCGGG AATCGCGTGA AGCGGAAACC CAATTCGTGG ATCATCCGTC AATTGGGGGA 
CCACCGAGCA GTATGGGTCC CACTAGTCTA ACGTCGACCG GGAGTCCCCA CAACAGCCTC 
GAGTACTCGC TGGTCAATAT CAACGGTTCC ATTGTCGGAG GGATTGGAAG CGAAAGGCAG 
CGGGATCTGG CCGTCAGTGC CGTTGTTTTG GACGAGTGGT TGAAGGAACT TGCGGCCATC 
ACTCAGGAGC AGTGCATTAT AATGATATCG GACCAGGTGT CATCCCAAAA GCCGGGGAAA 
CTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5566304 ( XM_021853491.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709183.1 Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAe128627
Clone ID Related Accession (Same CDS sequence)	XM_021853491.1 , XM_021853492.1 , XM_021853493.1
Accession Version	XM_021853491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	UPF0518 protein AAEL005291 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTCACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAACGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCGGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCCT GTCCCAGAAG 
AACTCCAACG TGGGAACGTA CATTGCAACA TACTCATCGA TCTGCCCGGT GTTGGTCACC 
GGGCTGGGTG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATCGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAG CTGCAGGAGC TGACACTGTT TATGAACTCG 
TTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCG GTGTTGGGTC CGGCCGTTCT CCAGACATTT 
AAAGGTCGAG GTCATTTGCA GACAAACGTC GAGTCGCAGA TTTCGGCGAT GGCCTATCTG 
GACCTGATCG TACGCTCGGT AACCGAACCG GGCCTGATTC AGATCGTGGT CAAGTTTCTA 
CTGGACGAGG AGAAGTTCGA CGGCCAGCGG ATATTGGACG TCCTGGTGGA GCGGCTCAAC 
AGCAACGACA CAAGGCTTTG CATGGTATCC TTATCACTAT TCGACACACT TCTGAGTCTC 
AACTGCGAGG ACATCATGCT GGAACTGGCC CTGAAGTACC TGCTCAACTG TCAACATGTG 
CCTATCTCGC ATCGGTTCAA GGTGAACCGG GTAGATCCAT ACAGCAACGC AGTCGAGTAC 
TTTCTGAACA CATCTCCGGA CATCATGAAA AAAGTGAACA ACGTGTTGAA CATCAACAAC 
AACAATAATA ACACCAGCCA AGCGTCGATG CTAGGGAGTG GCCCCTCATC TATGCCCCAG 
GGCGGACGAA ATGTTAGCAA AACGATCGGT GCCAACTGGA ACCATTACGG GATCAATACC 
GGAGAAACGC TTATGGCTAA CTACCAAGCG TACCTGTTGG AGGCCCGCAA CCGGATAGTT 
CAATGCAAAC ATGCCTGTGA TCAGTGGAAC AATGTGTATC GTTACCAAAA ATTGAGCAAA 
CTGGTAAACA ATAGTAGTAG TTTAAGTCAT AGCGGTAATG GAACGGCTGG GAGCGAAGAC 
GTCCGAATTT ACAAAGTACA AATGATCAAA AATTTCCTGG CGGAATTTAC CACCGCTCCG 
GACAGTGCGG TCGACATGGC GGGAGATGGT GACCGATCGA GTCAGTGCCA GTCTCCGGCG 
GGGATGTTCG GTACCGGGCA TCATGCGTCC CATCTGATGG TGACAACGTC GAAGCAGCTG 
GACAGTTTGC AATCGCTGGG CGATAGCAGC GGGTACGAAA GTTTGAACAT TACCAATTTG 
GGGACGGGCA GTGAAGATGG TCGGCGACAC GAAGCGTGGA AAGTTTCCAG CGTGAAGGAA 
GAAACCATTG TGGATTTGGA TCTGTCGGAA GATCTGTTCG CCCAGGGGAC CGTAAGTTTA 
GGTCCCTTCC TAACGGCCAT ATGGGGCAAA TTGCAAACGT TTACGAGCAA TTGCTTGTAC 
GTTAACCTTC ACCTGACCGG GCTGATAAGC CATCTGGCGT GGTTTCCTCT GCCGTTGCTG 
CACTCGATTC TGCTGCGACC CGACATACCG ACCACATCCG ATACGCCGTC GTTTCATCAG 
GTCCTGAAAA TTCTCAAACA ACAAATCGAC GCCGAGCTGC CCGACTGCGA CGAGTCGCTA 
GAAATCGTGG ACGTTGCCCG CTCCTTTCTC GTCGACCGAG AATTCCGACT CATCAATATG 
CGCAAGAATG CCATCGAATC GAACCCACAC AGTGGCAAAC TGTTGCTCCC GAATGGTGCA 
ACCGGAGCAT CCGGACCCGT ATCGATGACC TCCAACCTCT CGCAGACGAC ACCGATGCAG 
CTCACTCCGA GCTCCTCGTA CGATCCGTTC AAACGGAACG ACACCAAACG GAAAAGCATT 
TCCAATTCGT TTTCCAGTAT TTTCCGACGA CCAGGATCCT CCGCTTCATC TGGTCTAGCA 
CAAGTATTTC AATTTTTTAC CGGAGGCGGC TCCAGCAGCA ACAGCTCGTC GATCAGCCAT 
CAATCTTCCT CGACGCCATC GCCAGCCGGA TCCCAGCAGT ATCTTTCGTC CAACAGCTCC 
GGGGTAAGTA GCTTCATGGG AAGCAGTCGG CGGGAATCGC GTGAGGCGGA AACCCAATTC 
GTGGATCATC CGTCAATTGG GGGACCACCG AGCAGTATGG GTCCCACTAG TCTAACGTCG 
ACCGGGAGTC CCCACAACAG CCTCGAGTAC TCGCTGGTCA ATATCAACGG TGCCATCGTC 
GGAGGGATTG GAAGCGAAAG GCAGCGGGAT CTGGCCGTCA GTGCCGTTGT GTTGGACGAG 
TGGTTGAAGG AACTTGCGGC CATCACTCAG GAGCAGTGCA TTATAATGAT ATCGGACCAG 
GTGTCATCCC AAAAGCCGGG GAAACCCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709183.1
CDS	871..4023
Translation	MSWLRSSPLRQSFSKAGGGSGSGASSRGGNSTNGTVRAFDASECDPKACYDSFCIHWQQAFEIIQRSENNRGQSHDDVLGVVTHLDHMVTLLLVELHHCNKVGLPGAPAPPAPCLEHLLSENLLDKLYEWGVKTGRYANAVRLEQLKLYEQLVSHSRHQLLVHEPFLRPLLKILASSQNEIYPPDVEKRLVILLNQLCVVLMQNVHLLDLFFFSTAQQNGSGGHANFIIFSLLIPYVHREGSLGHQARDALLLCMALSQKNSNVGTYIATYSSICPVLVTGLGGLYSRLPNQIDIKTIDWYRITTDDVTELQELTLFMNSLEFCNAVVQVAHSMIRQQLLDFLYQGFLVPVLGPAVLQTFKGRGHLQTNVESQISAMAYLDLIVRSVTEPGLIQIVVKFLLDEEKFDGQRILDVLVERLNSNDTRLCMVSLSLFDTLLSLNCEDIMLELALKYLLNCQHVPISHRFKVNRVDPYSNAVEYFLNTSPDIMKKVNNVLNINNNNNNTSQASMLGSGPSSMPQGGRNVSKTIGANWNHYGINTGETLMANYQAYLLEARNRIVQCKHACDQWNNVYRYQKLSKLVNNSSSLSHSGNGTAGSEDVRIYKVQMIKNFLAEFTTAPDSAVDMAGDGDRSSQCQSPAGMFGTGHHASHLMVTTSKQLDSLQSLGDSSGYESLNITNLGTGSEDGRRHEAWKVSSVKEETIVDLDLSEDLFAQGTVSLGPFLTAIWGKLQTFTSNCLYVNLHLTGLISHLAWFPLPLLHSILLRPDIPTTSDTPSFHQVLKILKQQIDAELPDCDESLEIVDVARSFLVDREFRLINMRKNAIESNPHSGKLLLPNGATGASGPVSMTSNLSQTTPMQLTPSSSYDPFKRNDTKRKSISNSFSSIFRRPGSSASSGLAQVFQFFTGGGSSSNSSSISHQSSSTPSPAGSQQYLSSNSSGVSSFMGSSRRESREAETQFVDHPSIGGPPSSMGPTSLTSTGSPHNSLEYSLVNINGAIVGGIGSERQRDLAVSAVVLDEWLKELAAITQEQCIIMISDQVSSQKPGKPS

Target ORF information:

RefSeq Version	XM_021853491.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021853491.1

ORF Insert Sequence:

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTCACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAACGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCGGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCCT GTCCCAGAAG 
AACTCCAACG TGGGAACGTA CATTGCAACA TACTCATCGA TCTGCCCGGT GTTGGTCACC 
GGGCTGGGTG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATCGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAG CTGCAGGAGC TGACACTGTT TATGAACTCG 
TTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCG GTGTTGGGTC CGGCCGTTCT CCAGACATTT 
AAAGGTCGAG GTCATTTGCA GACAAACGTC GAGTCGCAGA TTTCGGCGAT GGCCTATCTG 
GACCTGATCG TACGCTCGGT AACCGAACCG GGCCTGATTC AGATCGTGGT CAAGTTTCTA 
CTGGACGAGG AGAAGTTCGA CGGCCAGCGG ATATTGGACG TCCTGGTGGA GCGGCTCAAC 
AGCAACGACA CAAGGCTTTG CATGGTATCC TTATCACTAT TCGACACACT TCTGAGTCTC 
AACTGCGAGG ACATCATGCT GGAACTGGCC CTGAAGTACC TGCTCAACTG TCAACATGTG 
CCTATCTCGC ATCGGTTCAA GGTGAACCGG GTAGATCCAT ACAGCAACGC AGTCGAGTAC 
TTTCTGAACA CATCTCCGGA CATCATGAAA AAAGTGAACA ACGTGTTGAA CATCAACAAC 
AACAATAATA ACACCAGCCA AGCGTCGATG CTAGGGAGTG GCCCCTCATC TATGCCCCAG 
GGCGGACGAA ATGTTAGCAA AACGATCGGT GCCAACTGGA ACCATTACGG GATCAATACC 
GGAGAAACGC TTATGGCTAA CTACCAAGCG TACCTGTTGG AGGCCCGCAA CCGGATAGTT 
CAATGCAAAC ATGCCTGTGA TCAGTGGAAC AATGTGTATC GTTACCAAAA ATTGAGCAAA 
CTGGTAAACA ATAGTAGTAG TTTAAGTCAT AGCGGTAATG GAACGGCTGG GAGCGAAGAC 
GTCCGAATTT ACAAAGTACA AATGATCAAA AATTTCCTGG CGGAATTTAC CACCGCTCCG 
GACAGTGCGG TCGACATGGC GGGAGATGGT GACCGATCGA GTCAGTGCCA GTCTCCGGCG 
GGGATGTTCG GTACCGGGCA TCATGCGTCC CATCTGATGG TGACAACGTC GAAGCAGCTG 
GACAGTTTGC AATCGCTGGG CGATAGCAGC GGGTACGAAA GTTTGAACAT TACCAATTTG 
GGGACGGGCA GTGAAGATGG TCGGCGACAC GAAGCGTGGA AAGTTTCCAG CGTGAAGGAA 
GAAACCATTG TGGATTTGGA TCTGTCGGAA GATCTGTTCG CCCAGGGGAC CGTAAGTTTA 
GGTCCCTTCC TAACGGCCAT ATGGGGCAAA TTGCAAACGT TTACGAGCAA TTGCTTGTAC 
GTTAACCTTC ACCTGACCGG GCTGATAAGC CATCTGGCGT GGTTTCCTCT GCCGTTGCTG 
CACTCGATTC TGCTGCGACC CGACATACCG ACCACATCCG ATACGCCGTC GTTTCATCAG 
GTCCTGAAAA TTCTCAAACA ACAAATCGAC GCCGAGCTGC CCGACTGCGA CGAGTCGCTA 
GAAATCGTGG ACGTTGCCCG CTCCTTTCTC GTCGACCGAG AATTCCGACT CATCAATATG 
CGCAAGAATG CCATCGAATC GAACCCACAC AGTGGCAAAC TGTTGCTCCC GAATGGTGCA 
ACCGGAGCAT CCGGACCCGT ATCGATGACC TCCAACCTCT CGCAGACGAC ACCGATGCAG 
CTCACTCCGA GCTCCTCGTA CGATCCGTTC AAACGGAACG ACACCAAACG GAAAAGCATT 
TCCAATTCGT TTTCCAGTAT TTTCCGACGA CCAGGATCCT CCGCTTCATC TGGTCTAGCA 
CAAGTATTTC AATTTTTTAC CGGAGGCGGC TCCAGCAGCA ACAGCTCGTC GATCAGCCAT 
CAATCTTCCT CGACGCCATC GCCAGCCGGA TCCCAGCAGT ATCTTTCGTC CAACAGCTCC 
GGGGTAAGTA GCTTCATGGG AAGCAGTCGG CGGGAATCGC GTGAGGCGGA AACCCAATTC 
GTGGATCATC CGTCAATTGG GGGACCACCG AGCAGTATGG GTCCCACTAG TCTAACGTCG 
ACCGGGAGTC CCCACAACAG CCTCGAGTAC TCGCTGGTCA ATATCAACGG TGCCATCGTC 
GGAGGGATTG GAAGCGAAAG GCAGCGGGAT CTGGCCGTCA GTGCCGTTGT GTTGGACGAG 
TGGTTGAAGG AACTTGCGGC CATCACTCAG GAGCAGTGCA TTATAATGAT ATCGGACCAG 
GTGTCATCCC AAAAGCCGGG GAAACCCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5566304 ( XM_021853492.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709184.1 Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAe128627
Clone ID Related Accession (Same CDS sequence)	XM_021853491.1 , XM_021853492.1 , XM_021853493.1
Accession Version	XM_021853492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	UPF0518 protein AAEL005291 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTCACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAACGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCGGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCCT GTCCCAGAAG 
AACTCCAACG TGGGAACGTA CATTGCAACA TACTCATCGA TCTGCCCGGT GTTGGTCACC 
GGGCTGGGTG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATCGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAG CTGCAGGAGC TGACACTGTT TATGAACTCG 
TTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCG GTGTTGGGTC CGGCCGTTCT CCAGACATTT 
AAAGGTCGAG GTCATTTGCA GACAAACGTC GAGTCGCAGA TTTCGGCGAT GGCCTATCTG 
GACCTGATCG TACGCTCGGT AACCGAACCG GGCCTGATTC AGATCGTGGT CAAGTTTCTA 
CTGGACGAGG AGAAGTTCGA CGGCCAGCGG ATATTGGACG TCCTGGTGGA GCGGCTCAAC 
AGCAACGACA CAAGGCTTTG CATGGTATCC TTATCACTAT TCGACACACT TCTGAGTCTC 
AACTGCGAGG ACATCATGCT GGAACTGGCC CTGAAGTACC TGCTCAACTG TCAACATGTG 
CCTATCTCGC ATCGGTTCAA GGTGAACCGG GTAGATCCAT ACAGCAACGC AGTCGAGTAC 
TTTCTGAACA CATCTCCGGA CATCATGAAA AAAGTGAACA ACGTGTTGAA CATCAACAAC 
AACAATAATA ACACCAGCCA AGCGTCGATG CTAGGGAGTG GCCCCTCATC TATGCCCCAG 
GGCGGACGAA ATGTTAGCAA AACGATCGGT GCCAACTGGA ACCATTACGG GATCAATACC 
GGAGAAACGC TTATGGCTAA CTACCAAGCG TACCTGTTGG AGGCCCGCAA CCGGATAGTT 
CAATGCAAAC ATGCCTGTGA TCAGTGGAAC AATGTGTATC GTTACCAAAA ATTGAGCAAA 
CTGGTAAACA ATAGTAGTAG TTTAAGTCAT AGCGGTAATG GAACGGCTGG GAGCGAAGAC 
GTCCGAATTT ACAAAGTACA AATGATCAAA AATTTCCTGG CGGAATTTAC CACCGCTCCG 
GACAGTGCGG TCGACATGGC GGGAGATGGT GACCGATCGA GTCAGTGCCA GTCTCCGGCG 
GGGATGTTCG GTACCGGGCA TCATGCGTCC CATCTGATGG TGACAACGTC GAAGCAGCTG 
GACAGTTTGC AATCGCTGGG CGATAGCAGC GGGTACGAAA GTTTGAACAT TACCAATTTG 
GGGACGGGCA GTGAAGATGG TCGGCGACAC GAAGCGTGGA AAGTTTCCAG CGTGAAGGAA 
GAAACCATTG TGGATTTGGA TCTGTCGGAA GATCTGTTCG CCCAGGGGAC CGTAAGTTTA 
GGTCCCTTCC TAACGGCCAT ATGGGGCAAA TTGCAAACGT TTACGAGCAA TTGCTTGTAC 
GTTAACCTTC ACCTGACCGG GCTGATAAGC CATCTGGCGT GGTTTCCTCT GCCGTTGCTG 
CACTCGATTC TGCTGCGACC CGACATACCG ACCACATCCG ATACGCCGTC GTTTCATCAG 
GTCCTGAAAA TTCTCAAACA ACAAATCGAC GCCGAGCTGC CCGACTGCGA CGAGTCGCTA 
GAAATCGTGG ACGTTGCCCG CTCCTTTCTC GTCGACCGAG AATTCCGACT CATCAATATG 
CGCAAGAATG CCATCGAATC GAACCCACAC AGTGGCAAAC TGTTGCTCCC GAATGGTGCA 
ACCGGAGCAT CCGGACCCGT ATCGATGACC TCCAACCTCT CGCAGACGAC ACCGATGCAG 
CTCACTCCGA GCTCCTCGTA CGATCCGTTC AAACGGAACG ACACCAAACG GAAAAGCATT 
TCCAATTCGT TTTCCAGTAT TTTCCGACGA CCAGGATCCT CCGCTTCATC TGGTCTAGCA 
CAAGTATTTC AATTTTTTAC CGGAGGCGGC TCCAGCAGCA ACAGCTCGTC GATCAGCCAT 
CAATCTTCCT CGACGCCATC GCCAGCCGGA TCCCAGCAGT ATCTTTCGTC CAACAGCTCC 
GGGGTAAGTA GCTTCATGGG AAGCAGTCGG CGGGAATCGC GTGAGGCGGA AACCCAATTC 
GTGGATCATC CGTCAATTGG GGGACCACCG AGCAGTATGG GTCCCACTAG TCTAACGTCG 
ACCGGGAGTC CCCACAACAG CCTCGAGTAC TCGCTGGTCA ATATCAACGG TGCCATCGTC 
GGAGGGATTG GAAGCGAAAG GCAGCGGGAT CTGGCCGTCA GTGCCGTTGT GTTGGACGAG 
TGGTTGAAGG AACTTGCGGC CATCACTCAG GAGCAGTGCA TTATAATGAT ATCGGACCAG 
GTGTCATCCC AAAAGCCGGG GAAACCCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709184.1
CDS	886..4038
Translation	MSWLRSSPLRQSFSKAGGGSGSGASSRGGNSTNGTVRAFDASECDPKACYDSFCIHWQQAFEIIQRSENNRGQSHDDVLGVVTHLDHMVTLLLVELHHCNKVGLPGAPAPPAPCLEHLLSENLLDKLYEWGVKTGRYANAVRLEQLKLYEQLVSHSRHQLLVHEPFLRPLLKILASSQNEIYPPDVEKRLVILLNQLCVVLMQNVHLLDLFFFSTAQQNGSGGHANFIIFSLLIPYVHREGSLGHQARDALLLCMALSQKNSNVGTYIATYSSICPVLVTGLGGLYSRLPNQIDIKTIDWYRITTDDVTELQELTLFMNSLEFCNAVVQVAHSMIRQQLLDFLYQGFLVPVLGPAVLQTFKGRGHLQTNVESQISAMAYLDLIVRSVTEPGLIQIVVKFLLDEEKFDGQRILDVLVERLNSNDTRLCMVSLSLFDTLLSLNCEDIMLELALKYLLNCQHVPISHRFKVNRVDPYSNAVEYFLNTSPDIMKKVNNVLNINNNNNNTSQASMLGSGPSSMPQGGRNVSKTIGANWNHYGINTGETLMANYQAYLLEARNRIVQCKHACDQWNNVYRYQKLSKLVNNSSSLSHSGNGTAGSEDVRIYKVQMIKNFLAEFTTAPDSAVDMAGDGDRSSQCQSPAGMFGTGHHASHLMVTTSKQLDSLQSLGDSSGYESLNITNLGTGSEDGRRHEAWKVSSVKEETIVDLDLSEDLFAQGTVSLGPFLTAIWGKLQTFTSNCLYVNLHLTGLISHLAWFPLPLLHSILLRPDIPTTSDTPSFHQVLKILKQQIDAELPDCDESLEIVDVARSFLVDREFRLINMRKNAIESNPHSGKLLLPNGATGASGPVSMTSNLSQTTPMQLTPSSSYDPFKRNDTKRKSISNSFSSIFRRPGSSASSGLAQVFQFFTGGGSSSNSSSISHQSSSTPSPAGSQQYLSSNSSGVSSFMGSSRRESREAETQFVDHPSIGGPPSSMGPTSLTSTGSPHNSLEYSLVNINGAIVGGIGSERQRDLAVSAVVLDEWLKELAAITQEQCIIMISDQVSSQKPGKPS

Target ORF information:

RefSeq Version	XM_021853492.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021853492.1

ORF Insert Sequence:

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTCACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAACGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCGGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCCT GTCCCAGAAG 
AACTCCAACG TGGGAACGTA CATTGCAACA TACTCATCGA TCTGCCCGGT GTTGGTCACC 
GGGCTGGGTG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATCGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAG CTGCAGGAGC TGACACTGTT TATGAACTCG 
TTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCG GTGTTGGGTC CGGCCGTTCT CCAGACATTT 
AAAGGTCGAG GTCATTTGCA GACAAACGTC GAGTCGCAGA TTTCGGCGAT GGCCTATCTG 
GACCTGATCG TACGCTCGGT AACCGAACCG GGCCTGATTC AGATCGTGGT CAAGTTTCTA 
CTGGACGAGG AGAAGTTCGA CGGCCAGCGG ATATTGGACG TCCTGGTGGA GCGGCTCAAC 
AGCAACGACA CAAGGCTTTG CATGGTATCC TTATCACTAT TCGACACACT TCTGAGTCTC 
AACTGCGAGG ACATCATGCT GGAACTGGCC CTGAAGTACC TGCTCAACTG TCAACATGTG 
CCTATCTCGC ATCGGTTCAA GGTGAACCGG GTAGATCCAT ACAGCAACGC AGTCGAGTAC 
TTTCTGAACA CATCTCCGGA CATCATGAAA AAAGTGAACA ACGTGTTGAA CATCAACAAC 
AACAATAATA ACACCAGCCA AGCGTCGATG CTAGGGAGTG GCCCCTCATC TATGCCCCAG 
GGCGGACGAA ATGTTAGCAA AACGATCGGT GCCAACTGGA ACCATTACGG GATCAATACC 
GGAGAAACGC TTATGGCTAA CTACCAAGCG TACCTGTTGG AGGCCCGCAA CCGGATAGTT 
CAATGCAAAC ATGCCTGTGA TCAGTGGAAC AATGTGTATC GTTACCAAAA ATTGAGCAAA 
CTGGTAAACA ATAGTAGTAG TTTAAGTCAT AGCGGTAATG GAACGGCTGG GAGCGAAGAC 
GTCCGAATTT ACAAAGTACA AATGATCAAA AATTTCCTGG CGGAATTTAC CACCGCTCCG 
GACAGTGCGG TCGACATGGC GGGAGATGGT GACCGATCGA GTCAGTGCCA GTCTCCGGCG 
GGGATGTTCG GTACCGGGCA TCATGCGTCC CATCTGATGG TGACAACGTC GAAGCAGCTG 
GACAGTTTGC AATCGCTGGG CGATAGCAGC GGGTACGAAA GTTTGAACAT TACCAATTTG 
GGGACGGGCA GTGAAGATGG TCGGCGACAC GAAGCGTGGA AAGTTTCCAG CGTGAAGGAA 
GAAACCATTG TGGATTTGGA TCTGTCGGAA GATCTGTTCG CCCAGGGGAC CGTAAGTTTA 
GGTCCCTTCC TAACGGCCAT ATGGGGCAAA TTGCAAACGT TTACGAGCAA TTGCTTGTAC 
GTTAACCTTC ACCTGACCGG GCTGATAAGC CATCTGGCGT GGTTTCCTCT GCCGTTGCTG 
CACTCGATTC TGCTGCGACC CGACATACCG ACCACATCCG ATACGCCGTC GTTTCATCAG 
GTCCTGAAAA TTCTCAAACA ACAAATCGAC GCCGAGCTGC CCGACTGCGA CGAGTCGCTA 
GAAATCGTGG ACGTTGCCCG CTCCTTTCTC GTCGACCGAG AATTCCGACT CATCAATATG 
CGCAAGAATG CCATCGAATC GAACCCACAC AGTGGCAAAC TGTTGCTCCC GAATGGTGCA 
ACCGGAGCAT CCGGACCCGT ATCGATGACC TCCAACCTCT CGCAGACGAC ACCGATGCAG 
CTCACTCCGA GCTCCTCGTA CGATCCGTTC AAACGGAACG ACACCAAACG GAAAAGCATT 
TCCAATTCGT TTTCCAGTAT TTTCCGACGA CCAGGATCCT CCGCTTCATC TGGTCTAGCA 
CAAGTATTTC AATTTTTTAC CGGAGGCGGC TCCAGCAGCA ACAGCTCGTC GATCAGCCAT 
CAATCTTCCT CGACGCCATC GCCAGCCGGA TCCCAGCAGT ATCTTTCGTC CAACAGCTCC 
GGGGTAAGTA GCTTCATGGG AAGCAGTCGG CGGGAATCGC GTGAGGCGGA AACCCAATTC 
GTGGATCATC CGTCAATTGG GGGACCACCG AGCAGTATGG GTCCCACTAG TCTAACGTCG 
ACCGGGAGTC CCCACAACAG CCTCGAGTAC TCGCTGGTCA ATATCAACGG TGCCATCGTC 
GGAGGGATTG GAAGCGAAAG GCAGCGGGAT CTGGCCGTCA GTGCCGTTGT GTTGGACGAG 
TGGTTGAAGG AACTTGCGGC CATCACTCAG GAGCAGTGCA TTATAATGAT ATCGGACCAG 
GTGTCATCCC AAAAGCCGGG GAAACCCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5566304 ( XM_021853493.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709185.1 Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAe128627
Clone ID Related Accession (Same CDS sequence)	XM_021853491.1 , XM_021853492.1 , XM_021853493.1
Accession Version	XM_021853493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	UPF0518 protein AAEL005291 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTCACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAACGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCGGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCCT GTCCCAGAAG 
AACTCCAACG TGGGAACGTA CATTGCAACA TACTCATCGA TCTGCCCGGT GTTGGTCACC 
GGGCTGGGTG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATCGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAG CTGCAGGAGC TGACACTGTT TATGAACTCG 
TTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCG GTGTTGGGTC CGGCCGTTCT CCAGACATTT 
AAAGGTCGAG GTCATTTGCA GACAAACGTC GAGTCGCAGA TTTCGGCGAT GGCCTATCTG 
GACCTGATCG TACGCTCGGT AACCGAACCG GGCCTGATTC AGATCGTGGT CAAGTTTCTA 
CTGGACGAGG AGAAGTTCGA CGGCCAGCGG ATATTGGACG TCCTGGTGGA GCGGCTCAAC 
AGCAACGACA CAAGGCTTTG CATGGTATCC TTATCACTAT TCGACACACT TCTGAGTCTC 
AACTGCGAGG ACATCATGCT GGAACTGGCC CTGAAGTACC TGCTCAACTG TCAACATGTG 
CCTATCTCGC ATCGGTTCAA GGTGAACCGG GTAGATCCAT ACAGCAACGC AGTCGAGTAC 
TTTCTGAACA CATCTCCGGA CATCATGAAA AAAGTGAACA ACGTGTTGAA CATCAACAAC 
AACAATAATA ACACCAGCCA AGCGTCGATG CTAGGGAGTG GCCCCTCATC TATGCCCCAG 
GGCGGACGAA ATGTTAGCAA AACGATCGGT GCCAACTGGA ACCATTACGG GATCAATACC 
GGAGAAACGC TTATGGCTAA CTACCAAGCG TACCTGTTGG AGGCCCGCAA CCGGATAGTT 
CAATGCAAAC ATGCCTGTGA TCAGTGGAAC AATGTGTATC GTTACCAAAA ATTGAGCAAA 
CTGGTAAACA ATAGTAGTAG TTTAAGTCAT AGCGGTAATG GAACGGCTGG GAGCGAAGAC 
GTCCGAATTT ACAAAGTACA AATGATCAAA AATTTCCTGG CGGAATTTAC CACCGCTCCG 
GACAGTGCGG TCGACATGGC GGGAGATGGT GACCGATCGA GTCAGTGCCA GTCTCCGGCG 
GGGATGTTCG GTACCGGGCA TCATGCGTCC CATCTGATGG TGACAACGTC GAAGCAGCTG 
GACAGTTTGC AATCGCTGGG CGATAGCAGC GGGTACGAAA GTTTGAACAT TACCAATTTG 
GGGACGGGCA GTGAAGATGG TCGGCGACAC GAAGCGTGGA AAGTTTCCAG CGTGAAGGAA 
GAAACCATTG TGGATTTGGA TCTGTCGGAA GATCTGTTCG CCCAGGGGAC CGTAAGTTTA 
GGTCCCTTCC TAACGGCCAT ATGGGGCAAA TTGCAAACGT TTACGAGCAA TTGCTTGTAC 
GTTAACCTTC ACCTGACCGG GCTGATAAGC CATCTGGCGT GGTTTCCTCT GCCGTTGCTG 
CACTCGATTC TGCTGCGACC CGACATACCG ACCACATCCG ATACGCCGTC GTTTCATCAG 
GTCCTGAAAA TTCTCAAACA ACAAATCGAC GCCGAGCTGC CCGACTGCGA CGAGTCGCTA 
GAAATCGTGG ACGTTGCCCG CTCCTTTCTC GTCGACCGAG AATTCCGACT CATCAATATG 
CGCAAGAATG CCATCGAATC GAACCCACAC AGTGGCAAAC TGTTGCTCCC GAATGGTGCA 
ACCGGAGCAT CCGGACCCGT ATCGATGACC TCCAACCTCT CGCAGACGAC ACCGATGCAG 
CTCACTCCGA GCTCCTCGTA CGATCCGTTC AAACGGAACG ACACCAAACG GAAAAGCATT 
TCCAATTCGT TTTCCAGTAT TTTCCGACGA CCAGGATCCT CCGCTTCATC TGGTCTAGCA 
CAAGTATTTC AATTTTTTAC CGGAGGCGGC TCCAGCAGCA ACAGCTCGTC GATCAGCCAT 
CAATCTTCCT CGACGCCATC GCCAGCCGGA TCCCAGCAGT ATCTTTCGTC CAACAGCTCC 
GGGGTAAGTA GCTTCATGGG AAGCAGTCGG CGGGAATCGC GTGAGGCGGA AACCCAATTC 
GTGGATCATC CGTCAATTGG GGGACCACCG AGCAGTATGG GTCCCACTAG TCTAACGTCG 
ACCGGGAGTC CCCACAACAG CCTCGAGTAC TCGCTGGTCA ATATCAACGG TGCCATCGTC 
GGAGGGATTG GAAGCGAAAG GCAGCGGGAT CTGGCCGTCA GTGCCGTTGT GTTGGACGAG 
TGGTTGAAGG AACTTGCGGC CATCACTCAG GAGCAGTGCA TTATAATGAT ATCGGACCAG 
GTGTCATCCC AAAAGCCGGG GAAACCCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709185.1
CDS	513..3665
Translation	MSWLRSSPLRQSFSKAGGGSGSGASSRGGNSTNGTVRAFDASECDPKACYDSFCIHWQQAFEIIQRSENNRGQSHDDVLGVVTHLDHMVTLLLVELHHCNKVGLPGAPAPPAPCLEHLLSENLLDKLYEWGVKTGRYANAVRLEQLKLYEQLVSHSRHQLLVHEPFLRPLLKILASSQNEIYPPDVEKRLVILLNQLCVVLMQNVHLLDLFFFSTAQQNGSGGHANFIIFSLLIPYVHREGSLGHQARDALLLCMALSQKNSNVGTYIATYSSICPVLVTGLGGLYSRLPNQIDIKTIDWYRITTDDVTELQELTLFMNSLEFCNAVVQVAHSMIRQQLLDFLYQGFLVPVLGPAVLQTFKGRGHLQTNVESQISAMAYLDLIVRSVTEPGLIQIVVKFLLDEEKFDGQRILDVLVERLNSNDTRLCMVSLSLFDTLLSLNCEDIMLELALKYLLNCQHVPISHRFKVNRVDPYSNAVEYFLNTSPDIMKKVNNVLNINNNNNNTSQASMLGSGPSSMPQGGRNVSKTIGANWNHYGINTGETLMANYQAYLLEARNRIVQCKHACDQWNNVYRYQKLSKLVNNSSSLSHSGNGTAGSEDVRIYKVQMIKNFLAEFTTAPDSAVDMAGDGDRSSQCQSPAGMFGTGHHASHLMVTTSKQLDSLQSLGDSSGYESLNITNLGTGSEDGRRHEAWKVSSVKEETIVDLDLSEDLFAQGTVSLGPFLTAIWGKLQTFTSNCLYVNLHLTGLISHLAWFPLPLLHSILLRPDIPTTSDTPSFHQVLKILKQQIDAELPDCDESLEIVDVARSFLVDREFRLINMRKNAIESNPHSGKLLLPNGATGASGPVSMTSNLSQTTPMQLTPSSSYDPFKRNDTKRKSISNSFSSIFRRPGSSASSGLAQVFQFFTGGGSSSNSSSISHQSSSTPSPAGSQQYLSSNSSGVSSFMGSSRRESREAETQFVDHPSIGGPPSSMGPTSLTSTGSPHNSLEYSLVNINGAIVGGIGSERQRDLAVSAVVLDEWLKELAAITQEQCIIMISDQVSSQKPGKPS

Target ORF information:

RefSeq Version	XM_021853493.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021853493.1

ORF Insert Sequence:

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTCACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAACGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCGGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCCT GTCCCAGAAG 
AACTCCAACG TGGGAACGTA CATTGCAACA TACTCATCGA TCTGCCCGGT GTTGGTCACC 
GGGCTGGGTG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATCGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAG CTGCAGGAGC TGACACTGTT TATGAACTCG 
TTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCG GTGTTGGGTC CGGCCGTTCT CCAGACATTT 
AAAGGTCGAG GTCATTTGCA GACAAACGTC GAGTCGCAGA TTTCGGCGAT GGCCTATCTG 
GACCTGATCG TACGCTCGGT AACCGAACCG GGCCTGATTC AGATCGTGGT CAAGTTTCTA 
CTGGACGAGG AGAAGTTCGA CGGCCAGCGG ATATTGGACG TCCTGGTGGA GCGGCTCAAC 
AGCAACGACA CAAGGCTTTG CATGGTATCC TTATCACTAT TCGACACACT TCTGAGTCTC 
AACTGCGAGG ACATCATGCT GGAACTGGCC CTGAAGTACC TGCTCAACTG TCAACATGTG 
CCTATCTCGC ATCGGTTCAA GGTGAACCGG GTAGATCCAT ACAGCAACGC AGTCGAGTAC 
TTTCTGAACA CATCTCCGGA CATCATGAAA AAAGTGAACA ACGTGTTGAA CATCAACAAC 
AACAATAATA ACACCAGCCA AGCGTCGATG CTAGGGAGTG GCCCCTCATC TATGCCCCAG 
GGCGGACGAA ATGTTAGCAA AACGATCGGT GCCAACTGGA ACCATTACGG GATCAATACC 
GGAGAAACGC TTATGGCTAA CTACCAAGCG TACCTGTTGG AGGCCCGCAA CCGGATAGTT 
CAATGCAAAC ATGCCTGTGA TCAGTGGAAC AATGTGTATC GTTACCAAAA ATTGAGCAAA 
CTGGTAAACA ATAGTAGTAG TTTAAGTCAT AGCGGTAATG GAACGGCTGG GAGCGAAGAC 
GTCCGAATTT ACAAAGTACA AATGATCAAA AATTTCCTGG CGGAATTTAC CACCGCTCCG 
GACAGTGCGG TCGACATGGC GGGAGATGGT GACCGATCGA GTCAGTGCCA GTCTCCGGCG 
GGGATGTTCG GTACCGGGCA TCATGCGTCC CATCTGATGG TGACAACGTC GAAGCAGCTG 
GACAGTTTGC AATCGCTGGG CGATAGCAGC GGGTACGAAA GTTTGAACAT TACCAATTTG 
GGGACGGGCA GTGAAGATGG TCGGCGACAC GAAGCGTGGA AAGTTTCCAG CGTGAAGGAA 
GAAACCATTG TGGATTTGGA TCTGTCGGAA GATCTGTTCG CCCAGGGGAC CGTAAGTTTA 
GGTCCCTTCC TAACGGCCAT ATGGGGCAAA TTGCAAACGT TTACGAGCAA TTGCTTGTAC 
GTTAACCTTC ACCTGACCGG GCTGATAAGC CATCTGGCGT GGTTTCCTCT GCCGTTGCTG 
CACTCGATTC TGCTGCGACC CGACATACCG ACCACATCCG ATACGCCGTC GTTTCATCAG 
GTCCTGAAAA TTCTCAAACA ACAAATCGAC GCCGAGCTGC CCGACTGCGA CGAGTCGCTA 
GAAATCGTGG ACGTTGCCCG CTCCTTTCTC GTCGACCGAG AATTCCGACT CATCAATATG 
CGCAAGAATG CCATCGAATC GAACCCACAC AGTGGCAAAC TGTTGCTCCC GAATGGTGCA 
ACCGGAGCAT CCGGACCCGT ATCGATGACC TCCAACCTCT CGCAGACGAC ACCGATGCAG 
CTCACTCCGA GCTCCTCGTA CGATCCGTTC AAACGGAACG ACACCAAACG GAAAAGCATT 
TCCAATTCGT TTTCCAGTAT TTTCCGACGA CCAGGATCCT CCGCTTCATC TGGTCTAGCA 
CAAGTATTTC AATTTTTTAC CGGAGGCGGC TCCAGCAGCA ACAGCTCGTC GATCAGCCAT 
CAATCTTCCT CGACGCCATC GCCAGCCGGA TCCCAGCAGT ATCTTTCGTC CAACAGCTCC 
GGGGTAAGTA GCTTCATGGG AAGCAGTCGG CGGGAATCGC GTGAGGCGGA AACCCAATTC 
GTGGATCATC CGTCAATTGG GGGACCACCG AGCAGTATGG GTCCCACTAG TCTAACGTCG 
ACCGGGAGTC CCCACAACAG CCTCGAGTAC TCGCTGGTCA ATATCAACGG TGCCATCGTC 
GGAGGGATTG GAAGCGAAAG GCAGCGGGAT CTGGCCGTCA GTGCCGTTGT GTTGGACGAG 
TGGTTGAAGG AACTTGCGGC CATCACTCAG GAGCAGTGCA TTATAATGAT ATCGGACCAG 
GTGTCATCCC AAAAGCCGGG GAAACCCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5566304 ( XM_001650633.2 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001650683.2 Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X4, mRNA.

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CloneID	OAe128628
Clone ID Related Accession (Same CDS sequence)	XM_001650633.2
Accession Version	XM_001650633.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	UPF0518 protein AAEL005291 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001650633.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTCACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAACGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCGGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCCT GTCCCAGAAG 
AACTCCAACG TGGGAACGTA CATTGCAACA TACTCATCGA TCTGCCCGGT GTTGGTCACC 
GGGCTGGGTG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATCGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAG CTGCAGGAGC TGACACTGTT TATGAACTCG 
TTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCG GTGTTGGGTC CGGCCGTTCT CCAGACAAAC 
GTCGAGTCGC AGATTTCGGC GATGGCCTAT CTGGACCTGA TCGTACGCTC GGTAACCGAA 
CCGGGCCTGA TTCAGATCGT GGTCAAGTTT CTACTGGACG AGGAGAAGTT CGACGGCCAG 
CGGATATTGG ACGTCCTGGT GGAGCGGCTC AACAGCAACG ACACAAGGCT TTGCATGGTA 
TCCTTATCAC TATTCGACAC ACTTCTGAGT CTCAACTGCG AGGACATCAT GCTGGAACTG 
GCCCTGAAGT ACCTGCTCAA CTGTCAACAT GTGCCTATCT CGCATCGGTT CAAGGTGAAC 
CGGGTAGATC CATACAGCAA CGCAGTCGAG TACTTTCTGA ACACATCTCC GGACATCATG 
AAAAAAGTGA ACAACGTGTT GAACATCAAC AACAACAATA ATAACACCAG CCAAGCGTCG 
ATGCTAGGGA GTGGCCCCTC ATCTATGCCC CAGGGCGGAC GAAATGTTAG CAAAACGATC 
GGTGCCAACT GGAACCATTA CGGGATCAAT ACCGGAGAAA CGCTTATGGC TAACTACCAA 
GCGTACCTGT TGGAGGCCCG CAACCGGATA GTTCAATGCA AACATGCCTG TGATCAGTGG 
AACAATGTGT ATCGTTACCA AAAATTGAGC AAACTGGTAA ACAATAGTAG TAGTTTAAGT 
CATAGCGGTA ATGGAACGGC TGGGAGCGAA GACGTCCGAA TTTACAAAGT ACAAATGATC 
AAAAATTTCC TGGCGGAATT TACCACCGCT CCGGACAGTG CGGTCGACAT GGCGGGAGAT 
GGTGACCGAT CGAGTCAGTG CCAGTCTCCG GCGGGGATGT TCGGTACCGG GCATCATGCG 
TCCCATCTGA TGGTGACAAC GTCGAAGCAG CTGGACAGTT TGCAATCGCT GGGCGATAGC 
AGCGGGTACG AAAGTTTGAA CATTACCAAT TTGGGGACGG GCAGTGAAGA TGGTCGGCGA 
CACGAAGCGT GGAAAGTTTC CAGCGTGAAG GAAGAAACCA TTGTGGATTT GGATCTGTCG 
GAAGATCTGT TCGCCCAGGG GACCGTAAGT TTAGGTCCCT TCCTAACGGC CATATGGGGC 
AAATTGCAAA CGTTTACGAG CAATTGCTTG TACGTTAACC TTCACCTGAC CGGGCTGATA 
AGCCATCTGG CGTGGTTTCC TCTGCCGTTG CTGCACTCGA TTCTGCTGCG ACCCGACATA 
CCGACCACAT CCGATACGCC GTCGTTTCAT CAGGTCCTGA AAATTCTCAA ACAACAAATC 
GACGCCGAGC TGCCCGACTG CGACGAGTCG CTAGAAATCG TGGACGTTGC CCGCTCCTTT 
CTCGTCGACC GAGAATTCCG ACTCATCAAT ATGCGCAAGA ATGCCATCGA ATCGAACCCA 
CACAGTGGCA AACTGTTGCT CCCGAATGGT GCAACCGGAG CATCCGGACC CGTATCGATG 
ACCTCCAACC TCTCGCAGAC GACACCGATG CAGCTCACTC CGAGCTCCTC GTACGATCCG 
TTCAAACGGA ACGACACCAA ACGGAAAAGC ATTTCCAATT CGTTTTCCAG TATTTTCCGA 
CGACCAGGAT CCTCCGCTTC ATCTGGTCTA GCACAAGTAT TTCAATTTTT TACCGGAGGC 
GGCTCCAGCA GCAACAGCTC GTCGATCAGC CATCAATCTT CCTCGACGCC ATCGCCAGCC 
GGATCCCAGC AGTATCTTTC GTCCAACAGC TCCGGGGTAA GTAGCTTCAT GGGAAGCAGT 
CGGCGGGAAT CGCGTGAGGC GGAAACCCAA TTCGTGGATC ATCCGTCAAT TGGGGGACCA 
CCGAGCAGTA TGGGTCCCAC TAGTCTAACG TCGACCGGGA GTCCCCACAA CAGCCTCGAG 
TACTCGCTGG TCAATATCAA CGGTGCCATC GTCGGAGGGA TTGGAAGCGA AAGGCAGCGG 
GATCTGGCCG TCAGTGCCGT TGTGTTGGAC GAGTGGTTGA AGGAACTTGC GGCCATCACT 
CAGGAGCAGT GCATTATAAT GATATCGGAC CAGGTGTCAT CCCAAAAGCC GGGGAAACCC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001650683.2
CDS	786..3911
Translation	MSWLRSSPLRQSFSKAGGGSGSGASSRGGNSTNGTVRAFDASECDPKACYDSFCIHWQQAFEIIQRSENNRGQSHDDVLGVVTHLDHMVTLLLVELHHCNKVGLPGAPAPPAPCLEHLLSENLLDKLYEWGVKTGRYANAVRLEQLKLYEQLVSHSRHQLLVHEPFLRPLLKILASSQNEIYPPDVEKRLVILLNQLCVVLMQNVHLLDLFFFSTAQQNGSGGHANFIIFSLLIPYVHREGSLGHQARDALLLCMALSQKNSNVGTYIATYSSICPVLVTGLGGLYSRLPNQIDIKTIDWYRITTDDVTELQELTLFMNSLEFCNAVVQVAHSMIRQQLLDFLYQGFLVPVLGPAVLQTNVESQISAMAYLDLIVRSVTEPGLIQIVVKFLLDEEKFDGQRILDVLVERLNSNDTRLCMVSLSLFDTLLSLNCEDIMLELALKYLLNCQHVPISHRFKVNRVDPYSNAVEYFLNTSPDIMKKVNNVLNINNNNNNTSQASMLGSGPSSMPQGGRNVSKTIGANWNHYGINTGETLMANYQAYLLEARNRIVQCKHACDQWNNVYRYQKLSKLVNNSSSLSHSGNGTAGSEDVRIYKVQMIKNFLAEFTTAPDSAVDMAGDGDRSSQCQSPAGMFGTGHHASHLMVTTSKQLDSLQSLGDSSGYESLNITNLGTGSEDGRRHEAWKVSSVKEETIVDLDLSEDLFAQGTVSLGPFLTAIWGKLQTFTSNCLYVNLHLTGLISHLAWFPLPLLHSILLRPDIPTTSDTPSFHQVLKILKQQIDAELPDCDESLEIVDVARSFLVDREFRLINMRKNAIESNPHSGKLLLPNGATGASGPVSMTSNLSQTTPMQLTPSSSYDPFKRNDTKRKSISNSFSSIFRRPGSSASSGLAQVFQFFTGGGSSSNSSSISHQSSSTPSPAGSQQYLSSNSSGVSSFMGSSRRESREAETQFVDHPSIGGPPSSMGPTSLTSTGSPHNSLEYSLVNINGAIVGGIGSERQRDLAVSAVVLDEWLKELAAITQEQCIIMISDQVSSQKPGKPS

Target ORF information:

RefSeq Version	XM_001650633.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001650633.2

ORF Insert Sequence:

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTCACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAACGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCGGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCCT GTCCCAGAAG 
AACTCCAACG TGGGAACGTA CATTGCAACA TACTCATCGA TCTGCCCGGT GTTGGTCACC 
GGGCTGGGTG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATCGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAG CTGCAGGAGC TGACACTGTT TATGAACTCG 
TTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCG GTGTTGGGTC CGGCCGTTCT CCAGACAAAC 
GTCGAGTCGC AGATTTCGGC GATGGCCTAT CTGGACCTGA TCGTACGCTC GGTAACCGAA 
CCGGGCCTGA TTCAGATCGT GGTCAAGTTT CTACTGGACG AGGAGAAGTT CGACGGCCAG 
CGGATATTGG ACGTCCTGGT GGAGCGGCTC AACAGCAACG ACACAAGGCT TTGCATGGTA 
TCCTTATCAC TATTCGACAC ACTTCTGAGT CTCAACTGCG AGGACATCAT GCTGGAACTG 
GCCCTGAAGT ACCTGCTCAA CTGTCAACAT GTGCCTATCT CGCATCGGTT CAAGGTGAAC 
CGGGTAGATC CATACAGCAA CGCAGTCGAG TACTTTCTGA ACACATCTCC GGACATCATG 
AAAAAAGTGA ACAACGTGTT GAACATCAAC AACAACAATA ATAACACCAG CCAAGCGTCG 
ATGCTAGGGA GTGGCCCCTC ATCTATGCCC CAGGGCGGAC GAAATGTTAG CAAAACGATC 
GGTGCCAACT GGAACCATTA CGGGATCAAT ACCGGAGAAA CGCTTATGGC TAACTACCAA 
GCGTACCTGT TGGAGGCCCG CAACCGGATA GTTCAATGCA AACATGCCTG TGATCAGTGG 
AACAATGTGT ATCGTTACCA AAAATTGAGC AAACTGGTAA ACAATAGTAG TAGTTTAAGT 
CATAGCGGTA ATGGAACGGC TGGGAGCGAA GACGTCCGAA TTTACAAAGT ACAAATGATC 
AAAAATTTCC TGGCGGAATT TACCACCGCT CCGGACAGTG CGGTCGACAT GGCGGGAGAT 
GGTGACCGAT CGAGTCAGTG CCAGTCTCCG GCGGGGATGT TCGGTACCGG GCATCATGCG 
TCCCATCTGA TGGTGACAAC GTCGAAGCAG CTGGACAGTT TGCAATCGCT GGGCGATAGC 
AGCGGGTACG AAAGTTTGAA CATTACCAAT TTGGGGACGG GCAGTGAAGA TGGTCGGCGA 
CACGAAGCGT GGAAAGTTTC CAGCGTGAAG GAAGAAACCA TTGTGGATTT GGATCTGTCG 
GAAGATCTGT TCGCCCAGGG GACCGTAAGT TTAGGTCCCT TCCTAACGGC CATATGGGGC 
AAATTGCAAA CGTTTACGAG CAATTGCTTG TACGTTAACC TTCACCTGAC CGGGCTGATA 
AGCCATCTGG CGTGGTTTCC TCTGCCGTTG CTGCACTCGA TTCTGCTGCG ACCCGACATA 
CCGACCACAT CCGATACGCC GTCGTTTCAT CAGGTCCTGA AAATTCTCAA ACAACAAATC 
GACGCCGAGC TGCCCGACTG CGACGAGTCG CTAGAAATCG TGGACGTTGC CCGCTCCTTT 
CTCGTCGACC GAGAATTCCG ACTCATCAAT ATGCGCAAGA ATGCCATCGA ATCGAACCCA 
CACAGTGGCA AACTGTTGCT CCCGAATGGT GCAACCGGAG CATCCGGACC CGTATCGATG 
ACCTCCAACC TCTCGCAGAC GACACCGATG CAGCTCACTC CGAGCTCCTC GTACGATCCG 
TTCAAACGGA ACGACACCAA ACGGAAAAGC ATTTCCAATT CGTTTTCCAG TATTTTCCGA 
CGACCAGGAT CCTCCGCTTC ATCTGGTCTA GCACAAGTAT TTCAATTTTT TACCGGAGGC 
GGCTCCAGCA GCAACAGCTC GTCGATCAGC CATCAATCTT CCTCGACGCC ATCGCCAGCC 
GGATCCCAGC AGTATCTTTC GTCCAACAGC TCCGGGGTAA GTAGCTTCAT GGGAAGCAGT 
CGGCGGGAAT CGCGTGAGGC GGAAACCCAA TTCGTGGATC ATCCGTCAAT TGGGGGACCA 
CCGAGCAGTA TGGGTCCCAC TAGTCTAACG TCGACCGGGA GTCCCCACAA CAGCCTCGAG 
TACTCGCTGG TCAATATCAA CGGTGCCATC GTCGGAGGGA TTGGAAGCGA AAGGCAGCGG 
GATCTGGCCG TCAGTGCCGT TGTGTTGGAC GAGTGGTTGA AGGAACTTGC GGCCATCACT 
CAGGAGCAGT GCATTATAAT GATATCGGAC CAGGTGTCAT CCCAAAAGCC GGGGAAACCC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5566304 ( XM_021853494.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021709186.1 Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X5, mRNA.

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CloneID	OAe128629
Clone ID Related Accession (Same CDS sequence)	XM_021853494.1
Accession Version	XM_021853494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	UPF0518 protein AAEL005291 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTCACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAACGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCGGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCCT GTCCCAGAAG 
AACTCCAACG TGGGAACGTA CATTGCAACA TACTCATCGA TCTGCCCGGT GTTGGTCACC 
GGGCTGGGTG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATCGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAG CTGCAGGAGC TGACACTGTT TATGAACTCG 
TTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCG GTGTTGGGTC CGGCCGTTCT CCAGACATTT 
AAAGGTCGAG GTCATTTGCA GACAAACGTC GAGTCGCAGA TTTCGGCGAT GGCCTATCTG 
GACCTGATCG TACGCTCGGT AACCGAACCG GGCCTGATTC AGATCGTGGT CAAGTTTCTA 
CTGGACGAGG AGAAGTTCGA CGGCCAGCGG ATATTGGACG TCCTGGTGGA GCGGCTCAAC 
AGCAACGACA CAAGGCTTTG CATGGTATCC TTATCACTAT TCGACACACT TCTGAGTCTC 
AACTGCGAGG ACATCATGCT GGAACTGGCC CTGAAGTACC TGCTCAACTG TCAACATGTG 
CCTATCTCGC ATCGGTTCAA GGTGAACCGG GTAGATCCAT ACAGCAACGC AGTCGAGTAC 
TTTCTGAACA CATCTCCGGA CATCATGAAA AAAGTGAACA ACGTGTTGAA CATCAACAAC 
AACAATAATA ACACCAGCCA AGCGTCGATG CTAGGGAGTG GCCCCTCATC TATGCCCCAG 
GGCGGACGAA ATGTTAGCAA AACGATCGGT GCCAACTGGA ACCATTACGG GATCAATACC 
GGAGAAACGC TTATGGCTAA CTACCAAGCG TACCTGTTGG AGGCCCGCAA CCGGATAGTT 
CAATGCAAAC ATGCCTGTGA TCAGTGGAAC AATGTGTATC GTTACCAAAA ATTGAGCAAA 
CTGGTAAACA ATAGTAGTAG TTTAAGTCAT AGCGGTAATG GAACGGCTGG GAGCGAAGAC 
GTCCGAATTT ACAAAGTACA AATGATCAAA AATTTCCTGG CGGAATTTAC CACCGCTCCG 
GACAGTGCGG TCGACATGGC GGGAGATGGT GACCGATCGA GTCAGTGCCA GTCTCCGGCG 
GGGATGTTCG GTACCGGGCA TCATGCGTCC CATCTGATGG TGACAACGTC GAAGCAGCTG 
GACAGTTTGC AATCGCTGGG CGATAGCAGC GGGTACGAAA GTTTGAACAT TACCAATTTG 
GGGACGGGCA GTGAAGATGG TCGGCGACAC GAAGCGTGGA AAGTTTCCAG CGTGAAGGAA 
GAAACCATTG TGGATTTGGA TCTGTCGGAA GATCTGTTCG CCCAGGGGAC CGTAAGTTTA 
GGTCCCTTCC TAACGGCCAT ATGGGGCAAA TTGCAAACGT TTACGAGCAA TTGCTTGTAC 
GTTAACCTTC ACCTGACCGG GCTGATAAGC CATCTGGCGT GGTTTCCTCT GCCGTTGCTG 
CACTCGATTC TGCTGCGACC CGACATACCG ACCACATCCG ATACGCCGTC GTTTCATCAG 
GTCCTGAAAA TTCTCAAACA ACAAATCGAC GCCGAGCTGC CCGACTGCGA CGAGTCGCTA 
GAAATCGTGG ACGTTGCCCG CTCCTTTCTC GTCGACCGAG AATTCCGACT CATCAATATG 
CGCAAGAATG CCATCGAATC GAACCCACAC AGTGGCAAAC TGTTGCTCCC GAATGGTGCA 
ACCGGAGCAT CCGGACCCGT ATCGATGACC TCCAACCTCT CGCAGACGAC ACCGATGCAG 
CTCACTCCGA GCTCCTCGTA CGATCCGTTC AAACGGAACG ACACCAAACG GAAAAGCATT 
TCCAATTCGT TTTCCAGTAT TTTCCGACGA CCAGGATCCT CCGGAGGCGG CTCCAGCAGC 
AACAGCTCGT CGATCAGCCA TCAATCTTCC TCGACGCCAT CGCCAGCCGG ATCCCAGCAG 
TATCTTTCGT CCAACAGCTC CGGGGTAAGT AGCTTCATGG GAAGCAGTCG GCGGGAATCG 
CGTGAGGCGG AAACCCAATT CGTGGATCAT CCGTCAATTG GGGGACCACC GAGCAGTATG 
GGTCCCACTA GTCTAACGTC GACCGGGAGT CCCCACAACA GCCTCGAGTA CTCGCTGGTC 
AATATCAACG GTGCCATCGT CGGAGGGATT GGAAGCGAAA GGCAGCGGGA TCTGGCCGTC 
AGTGCCGTTG TGTTGGACGA GTGGTTGAAG GAACTTGCGG CCATCACTCA GGAGCAGTGC 
ATTATAATGA TATCGGACCA GGTGTCATCC CAAAAGCCGG GGAAACCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021709186.1
CDS	871..3984
Translation	MSWLRSSPLRQSFSKAGGGSGSGASSRGGNSTNGTVRAFDASECDPKACYDSFCIHWQQAFEIIQRSENNRGQSHDDVLGVVTHLDHMVTLLLVELHHCNKVGLPGAPAPPAPCLEHLLSENLLDKLYEWGVKTGRYANAVRLEQLKLYEQLVSHSRHQLLVHEPFLRPLLKILASSQNEIYPPDVEKRLVILLNQLCVVLMQNVHLLDLFFFSTAQQNGSGGHANFIIFSLLIPYVHREGSLGHQARDALLLCMALSQKNSNVGTYIATYSSICPVLVTGLGGLYSRLPNQIDIKTIDWYRITTDDVTELQELTLFMNSLEFCNAVVQVAHSMIRQQLLDFLYQGFLVPVLGPAVLQTFKGRGHLQTNVESQISAMAYLDLIVRSVTEPGLIQIVVKFLLDEEKFDGQRILDVLVERLNSNDTRLCMVSLSLFDTLLSLNCEDIMLELALKYLLNCQHVPISHRFKVNRVDPYSNAVEYFLNTSPDIMKKVNNVLNINNNNNNTSQASMLGSGPSSMPQGGRNVSKTIGANWNHYGINTGETLMANYQAYLLEARNRIVQCKHACDQWNNVYRYQKLSKLVNNSSSLSHSGNGTAGSEDVRIYKVQMIKNFLAEFTTAPDSAVDMAGDGDRSSQCQSPAGMFGTGHHASHLMVTTSKQLDSLQSLGDSSGYESLNITNLGTGSEDGRRHEAWKVSSVKEETIVDLDLSEDLFAQGTVSLGPFLTAIWGKLQTFTSNCLYVNLHLTGLISHLAWFPLPLLHSILLRPDIPTTSDTPSFHQVLKILKQQIDAELPDCDESLEIVDVARSFLVDREFRLINMRKNAIESNPHSGKLLLPNGATGASGPVSMTSNLSQTTPMQLTPSSSYDPFKRNDTKRKSISNSFSSIFRRPGSSGGGSSSNSSSISHQSSSTPSPAGSQQYLSSNSSGVSSFMGSSRRESREAETQFVDHPSIGGPPSSMGPTSLTSTGSPHNSLEYSLVNINGAIVGGIGSERQRDLAVSAVVLDEWLKELAAITQEQCIIMISDQVSSQKPGKPS

Target ORF information:

RefSeq Version	XM_021853494.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti UPF0518 protein AAEL005291 (LOC5566304), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021853494.1

ORF Insert Sequence:

ATGAGCTGGC TTCGGTCCAG CCCGCTGCGG CAAAGTTTCA GCAAAGCCGG TGGAGGCAGC 
GGAAGTGGCG CCAGCAGTCG CGGAGGCAAC TCCACTAACG GAACCGTCCG GGCGTTTGAT 
GCGTCTGAGT GCGATCCGAA GGCGTGCTAC GATAGTTTCT GTATCCACTG GCAGCAGGCG 
TTCGAGATCA TCCAGCGGTC GGAGAACAAT CGTGGCCAAT CGCATGATGA CGTCCTCGGA 
GTGGTCACCC ATCTGGATCA CATGGTCACA CTGTTGCTGG TGGAGTTACA TCACTGCAAT 
AAGGTAGGCC TGCCAGGAGC TCCCGCACCT CCCGCACCCT GCCTGGAACA CTTGCTAAGC 
GAGAACCTAC TCGACAAGCT CTACGAATGG GGCGTCAAAA CAGGAAGGTA TGCCAATGCC 
GTCCGGTTGG AGCAGCTCAA GCTGTACGAA CAGCTAGTCA GTCACAGTCG ACATCAACTG 
CTGGTGCATG AACCGTTCCT GCGGCCTCTG CTCAAAATAT TGGCATCCAG TCAGAATGAA 
ATCTATCCAC CGGATGTGGA GAAACGACTA GTGATACTGC TCAACCAGCT CTGCGTGGTG 
CTGATGCAGA ACGTGCACCT GTTGGATCTG TTCTTCTTCT CGACAGCCCA GCAGAACGGA 
AGTGGAGGGC ATGCAAACTT TATAATCTTC TCCCTGCTAA TACCGTACGT TCACCGGGAG 
GGCTCGTTGG GTCACCAGGC TCGCGATGCG CTACTGCTGT GCATGGCCCT GTCCCAGAAG 
AACTCCAACG TGGGAACGTA CATTGCAACA TACTCATCGA TCTGCCCGGT GTTGGTCACC 
GGGCTGGGTG GATTGTACTC GCGCTTGCCA AACCAAATCG ACATCAAAAC CATCGATTGG 
TACCGGATAA CGACGGATGA CGTGACCGAG CTGCAGGAGC TGACACTGTT TATGAACTCG 
TTGGAGTTCT GCAACGCTGT GGTCCAGGTG GCACACAGCA TGATTCGGCA GCAGTTGCTG 
GACTTTTTGT ACCAAGGGTT TTTGGTACCG GTGTTGGGTC CGGCCGTTCT CCAGACATTT 
AAAGGTCGAG GTCATTTGCA GACAAACGTC GAGTCGCAGA TTTCGGCGAT GGCCTATCTG 
GACCTGATCG TACGCTCGGT AACCGAACCG GGCCTGATTC AGATCGTGGT CAAGTTTCTA 
CTGGACGAGG AGAAGTTCGA CGGCCAGCGG ATATTGGACG TCCTGGTGGA GCGGCTCAAC 
AGCAACGACA CAAGGCTTTG CATGGTATCC TTATCACTAT TCGACACACT TCTGAGTCTC 
AACTGCGAGG ACATCATGCT GGAACTGGCC CTGAAGTACC TGCTCAACTG TCAACATGTG 
CCTATCTCGC ATCGGTTCAA GGTGAACCGG GTAGATCCAT ACAGCAACGC AGTCGAGTAC 
TTTCTGAACA CATCTCCGGA CATCATGAAA AAAGTGAACA ACGTGTTGAA CATCAACAAC 
AACAATAATA ACACCAGCCA AGCGTCGATG CTAGGGAGTG GCCCCTCATC TATGCCCCAG 
GGCGGACGAA ATGTTAGCAA AACGATCGGT GCCAACTGGA ACCATTACGG GATCAATACC 
GGAGAAACGC TTATGGCTAA CTACCAAGCG TACCTGTTGG AGGCCCGCAA CCGGATAGTT 
CAATGCAAAC ATGCCTGTGA TCAGTGGAAC AATGTGTATC GTTACCAAAA ATTGAGCAAA 
CTGGTAAACA ATAGTAGTAG TTTAAGTCAT AGCGGTAATG GAACGGCTGG GAGCGAAGAC 
GTCCGAATTT ACAAAGTACA AATGATCAAA AATTTCCTGG CGGAATTTAC CACCGCTCCG 
GACAGTGCGG TCGACATGGC GGGAGATGGT GACCGATCGA GTCAGTGCCA GTCTCCGGCG 
GGGATGTTCG GTACCGGGCA TCATGCGTCC CATCTGATGG TGACAACGTC GAAGCAGCTG 
GACAGTTTGC AATCGCTGGG CGATAGCAGC GGGTACGAAA GTTTGAACAT TACCAATTTG 
GGGACGGGCA GTGAAGATGG TCGGCGACAC GAAGCGTGGA AAGTTTCCAG CGTGAAGGAA 
GAAACCATTG TGGATTTGGA TCTGTCGGAA GATCTGTTCG CCCAGGGGAC CGTAAGTTTA 
GGTCCCTTCC TAACGGCCAT ATGGGGCAAA TTGCAAACGT TTACGAGCAA TTGCTTGTAC 
GTTAACCTTC ACCTGACCGG GCTGATAAGC CATCTGGCGT GGTTTCCTCT GCCGTTGCTG 
CACTCGATTC TGCTGCGACC CGACATACCG ACCACATCCG ATACGCCGTC GTTTCATCAG 
GTCCTGAAAA TTCTCAAACA ACAAATCGAC GCCGAGCTGC CCGACTGCGA CGAGTCGCTA 
GAAATCGTGG ACGTTGCCCG CTCCTTTCTC GTCGACCGAG AATTCCGACT CATCAATATG 
CGCAAGAATG CCATCGAATC GAACCCACAC AGTGGCAAAC TGTTGCTCCC GAATGGTGCA 
ACCGGAGCAT CCGGACCCGT ATCGATGACC TCCAACCTCT CGCAGACGAC ACCGATGCAG 
CTCACTCCGA GCTCCTCGTA CGATCCGTTC AAACGGAACG ACACCAAACG GAAAAGCATT 
TCCAATTCGT TTTCCAGTAT TTTCCGACGA CCAGGATCCT CCGGAGGCGG CTCCAGCAGC 
AACAGCTCGT CGATCAGCCA TCAATCTTCC TCGACGCCAT CGCCAGCCGG ATCCCAGCAG 
TATCTTTCGT CCAACAGCTC CGGGGTAAGT AGCTTCATGG GAAGCAGTCG GCGGGAATCG 
CGTGAGGCGG AAACCCAATT CGTGGATCAT CCGTCAATTG GGGGACCACC GAGCAGTATG 
GGTCCCACTA GTCTAACGTC GACCGGGAGT CCCCACAACA GCCTCGAGTA CTCGCTGGTC 
AATATCAACG GTGCCATCGT CGGAGGGATT GGAAGCGAAA GGCAGCGGGA TCTGGCCGTC 
AGTGCCGTTG TGTTGGACGA GTGGTTGAAG GAACTTGCGG CCATCACTCA GGAGCAGTGC 
ATTATAATGA TATCGGACCA GGTGTCATCC CAAAAGCCGG GGAAACCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5566304 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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