AAEL_AAEL004327 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL004327
Entrez Gene ID	5564593
Full Name	valine--tRNA ligase
Synonyms	AAEL004327
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001648875.2	XP_001648925.2	valine--tRNA ligase
XM_001648875.1	XP_001648925.1	AAEL004327-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL004327 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL004327 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAe00906	XM_001648875.1 Latest version!	Aedes aegypti AAEL004327-RA mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAe125085	XM_001648875.2 Latest version!	Aedes aegypti valine--tRNA ligase (LOC5564593), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL004327 ( XM_001648875.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001648925.1 Aedes aegypti AAEL004327-RA mRNA.

Price & Availability↑

CloneID	OAe00906
Clone ID Related Accession (Same CDS sequence)	XM_001648875.1
Accession Version	XM_001648875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1425398400000
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL004327-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001809968).

ATGTCCGATC CGGTGGAAAA TGGTACCGCT GCGGCCGAGT CCGGAACAGC TCCGGTCAAG 
ACGGAAAAGC AGCTCAAAAA GGAAGCGGAA AAGGCTGCCA AGTTGGCCAA GCTACAGGAG 
AAAATGAACA AAAAACAGCA GCAGGCGGAA CAGGCCGCCG TCAAGCCCAA AGCTGAGAAA 
AAAGCCAAGG AGACTGTGAA GGAGGCAATT GTATACAAAG GCAATACTCA GGAGGGCGAC 
AAGAAAGACC TGTCCGGTCC GTTCCCGGAA GCCTACAGTC CGCAATACGT GGAAGCTGCC 
TGGTACAGCT GGTGGGAGAA GGAAGGATTC TTCAAGCCTG AATATGCGCG CAAGGAGAAC 
AATCCCAACG GGCAGTTTGT GATGGTGATT CCGCCGCCGA ATGTGACCGG TTCGTTGCAT 
TTAGGTCACG CACTGACGAA CGCCATCGAG GACTGCATCA CGCGTTGGAA CCGGATGAAG 
GGCCGCACCA CGTTGTGGGT CCCGGGATGT GATCATGCTG GAATTGCCAC CCAGGTCGTG 
GTGGAGAAGA AATTGTGGCG CGAGCAGAAG CAAACCAGAC ACGATTTGGG CCGGGAGAAG 
TTCATCGAAA AGATTTGGCA GTGGCGCAAT GAGAAGGGTG ATCGTATCTA TCATCAGCTG 
AAGAGGCTGG GATCGTCGTA CGACTGGTCC AGAGCTTGTT TTACGATGGA TCCCAAGCTG 
TGCAAGGCCG TGACGGAAGC TTTTGTTCGA TTGCATGAGC GCGGAATGAT CTACAGGAGC 
AACCGTTTGG GTAATTGGTC ATGTGCGTTG CGATCGGCCA TTTCCGACAT TGAAGTAGAT 
AAGGTGGAAA TTCCTGGCAG GACCCTGCTG GCCATTCCGG GCTATACCGA AAAGGTTGAG 
TTTGGTGTGT TGGTGTCGTT TGCCTACAAA GTGGAAGGTA GCGACGAAGA GATCGTTGTG 
GCGACCACTC GTGTAGAGAC AATGCTTGGA GATACGGCCG TTGCCGTCCA TCCAAAGGAC 
GAACGCTACA AGCATCTACA CGGAAAGTTT GTAGTTCATC CGTTCTGCGA TCGTAAACTA 
CCGATTGTTT GCGATGATTT CGTTGAAATG GGCTTCGGAA CGGGCGCCGT CAAGATTACT 
CCAGCTCACG ATCCGAACGA TTATGAAGTT GGAAAACGTC ACAATTTGCC GTTCATTACC 
ATTTTCACGG ATGATGGTTT CATCACCGGG GAGTACGGAG AATTCACCGG AATGAAACGC 
TTCGATGCTA GGAAGGCTAT TCTGGTTGCT CTCCAGGCTA AGGGACTCTA CAAGGAAACC 
GTTGACAATC CGATGGTTGT GCCGATCTGT TCCCGCTCGA AGGATGTTGT GGAACCGCTG 
ATTAAGCCCC AGTGGTACGT CAAGTGTGAC GAGATGGCCA AGAACGCGAC GGAAGCGGTT 
CGCTCTGGAG AGCTGAAGAG CACGCCGGAA GTGCACAAGA AGAATTGGTA CCACTGGATG 
GATGAGATCC GCGATGGGTG TGTTTCTCGC CAACTTTGGG GGGGTCATAG AATCCCCGCC 
TATCAGGCTT CCTTCAAGGA CCCCAGTCAG AAGCCGGCGG GCCTTGACGC GGAATCATTA 
TGGGCCGTTG GGCGCACCGA GGTAGAAGCC TTGGAGAAGG CCGCGAAGGA GTTGAACGTT 
TCTAAAGAGG CTCGGGCTCT GAAGCAAGAC GAAGACGTCC GGGATACTTG GTTCAGCTCT 
GGTCTTTTCC CATTCTCTGT TGTCGGCTGG CCCGACAATA CCGTCGATGT GAAACTCTAC 
TACCCAACCT CCCTACTCGA GACGGGACAC GATATTTTGT TCTTCTGGGT GGCTGGCATG 
GTGTTCTTTG GCCAGACTTT GCTCGGCAAG CTTCCCTTCA AAGAAGTGTT CCTCCATCCG 
ATGGTCCGCG ATGCGCACGG CCGCAAAATG TCCAAATCGT TGGGTAATGT CATTGACCCG 
ATGGATGTGA TTACGGGAAT TTCCCTGGAC GGACTTCACC TGCAGCTGCT GGATTCGAAC 
CTGGACCCAA AGGAGATCGA GAAGGCGAAG GCCGGCCAGA AGCAGGACTA TCCTAACGGT 
ATTCCGGAAT GTGGAACCGA TGCTATGCGG TTCGCTCTGT GCGCCTACAA CACCCAGGGC 
CGGGACATTA ATTTGGACAT CATGCGCGTG CAGGGATATC GATTCTTCTG CAATAAGTTG 
TGGAACGCCA CTAAGTTCGC GCTGATGTAC TTCACCGGGG AAGAGCGCTA TGATGTGCTG 
CAAGAATTGA CTGGTGCCGA GAGCAACATT GACCGCTGGA TACTGTCCCG TCTGGCCAAC 
TGCATCGACG TTTCCAACAA GGGTTTCGAG AAGTACGAAT TTGCGCAAAC CACTAGCGCG 
TGCTACGAGT TCTGGCTGTA CGATCTGTGT GACGTCTATC TGGAATGTTT GAAACCCGTT 
TTCCAGTCCG GTTCGGAAGA AGCCAAGGCC TCTGCTCGGA AGACACTCTA TACCTGTTTG 
AATCTTGGCC TGAAACTGCT GTCGCCGTTT ATGCCGTTCA TTACCGAGGA ACTGTACCAG 
CGGCTGCCAC GTGCTGATGT CGAGCAGGTG GCCAGTATTT GCGTGGCTCC CTATCCGGAA 
GTGGAAAGCT CTAACTGGCA TAATGAACAA CTTGAGAAGG ACTTTGAATT TGTGCAACGC 
GTTGCAAAAG TTATCCGATC GGCCCGCAGC GATTACAACT TGCCGAACAA GACAAAAACC 
GAGGCGTTTG TTCTGTGCAG CGATGATGCC ACGAAGGATA CCCTGGAACG CTTTGCTGGG 
GATTTGACTA CCATGTCCTA CTCGCAGATT AGCCTCATCA GCGATGCTCC TCCAGCGGGA 
TGCGCTATTT TGACTGTGTC CGGAGCTTGT GTTGTTCATC TGCTTCTTAA GGGACTTATC 
GAGGTGGACA AGGAAGTGGA GAAACTTAAC AAGAAGAAAG ACAACTTGAG CTCAACCGTT 
GGCAAATTGG AACAGGCGAT GGGAGCGGCC GATTATGAAA CGAAGGTCCC CGAGGAAGTT 
CGGAACAACA ACCGGGAAAA GTTGGATCAG TCGAAAATCG AAATTGAACG TATCATTGCA 
GCAATAGAAA CCCTGAAGAT GATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001648925.1
CDS	141..3287
Translation	MSDPVENGTAAAESGTAPVKTEKQLKKEAEKAAKLAKLQEKMNKKQQQAEQAAVKPKAEKKAKETVKEAIVYKGNTQEGDKKDLSGPFPEAYSPQYVEAAWYSWWEKEGFFKPEYARKENNPNGQFVMVIPPPNVTGSLHLGHALTNAIEDCITRWNRMKGRTTLWVPGCDHAGIATQVVVEKKLWREQKQTRHDLGREKFIEKIWQWRNEKGDRIYHQLKRLGSSYDWSRACFTMDPKLCKAVTEAFVRLHERGMIYRSNRLGNWSCALRSAISDIEVDKVEIPGRTLLAIPGYTEKVEFGVLVSFAYKVEGSDEEIVVATTRVETMLGDTAVAVHPKDERYKHLHGKFVVHPFCDRKLPIVCDDFVEMGFGTGAVKITPAHDPNDYEVGKRHNLPFITIFTDDGFITGEYGEFTGMKRFDARKAILVALQAKGLYKETVDNPMVVPICSRSKDVVEPLIKPQWYVKCDEMAKNATEAVRSGELKSTPEVHKKNWYHWMDEIRDGCVSRQLWGGHRIPAYQASFKDPSQKPAGLDAESLWAVGRTEVEALEKAAKELNVSKEARALKQDEDVRDTWFSSGLFPFSVVGWPDNTVDVKLYYPTSLLETGHDILFFWVAGMVFFGQTLLGKLPFKEVFLHPMVRDAHGRKMSKSLGNVIDPMDVITGISLDGLHLQLLDSNLDPKEIEKAKAGQKQDYPNGIPECGTDAMRFALCAYNTQGRDINLDIMRVQGYRFFCNKLWNATKFALMYFTGEERYDVLQELTGAESNIDRWILSRLANCIDVSNKGFEKYEFAQTTSACYEFWLYDLCDVYLECLKPVFQSGSEEAKASARKTLYTCLNLGLKLLSPFMPFITEELYQRLPRADVEQVASICVAPYPEVESSNWHNEQLEKDFEFVQRVAKVIRSARSDYNLPNKTKTEAFVLCSDDATKDTLERFAGDLTTMSYSQISLISDAPPAGCAILTVSGACVVHLLLKGLIEVDKEVEKLNKKKDNLSSTVGKLEQAMGAADYETKVPEEVRNNNREKLDQSKIEIERIIAAIETLKMM

Target ORF information:

RefSeq Version	XM_001648875.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL004327-RA mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001648875.1

ORF Insert Sequence:

ATGTCCGATC CGGTGGAAAA TGGTACCGCT GCGGCCGAGT CCGGAACAGC TCCGGTCAAG 
ACGGAAAAGC AGCTCAAAAA GGAAGCGGAA AAGGCTGCCA AGTTGGCCAA GCTACAGGAG 
AAAATGAACA AAAAACAGCA GCAGGCGGAA CAGGCCGCCG TCAAGCCCAA AGCTGAGAAA 
AAAGCCAAGG AGACTGTGAA GGAGGCAATT GTATACAAAG GCAATACTCA GGAGGGCGAC 
AAGAAAGACC TGTCCGGTCC GTTCCCGGAA GCCTACAGTC CGCAATACGT GGAAGCTGCC 
TGGTACAGCT GGTGGGAGAA GGAAGGATTC TTCAAGCCTG AATATGCGCG CAAGGAGAAC 
AATCCCAACG GGCAGTTTGT GATGGTGATT CCGCCGCCGA ATGTGACCGG TTCGTTGCAT 
TTAGGTCACG CACTGACGAA CGCCATCGAG GACTGCATCA CGCGTTGGAA CCGGATGAAG 
GGCCGCACCA CGTTGTGGGT CCCGGGATGT GATCATGCTG GAATTGCCAC CCAGGTCGTG 
GTGGAGAAGA AATTGTGGCG CGAGCAGAAG CAAACCAGAC ACGATTTGGG CCGGGAGAAG 
TTCATCGAAA AGATTTGGCA GTGGCGCAAT GAGAAGGGTG ATCGTATCTA TCATCAGCTG 
AAGAGGCTGG GATCGTCGTA CGACTGGTCC AGAGCTTGTT TTACGATGGA TCCCAAGCTG 
TGCAAGGCCG TGACGGAAGC TTTTGTTCGA TTGCATGAGC GCGGAATGAT CTACAGGAGC 
AACCGTTTGG GTAATTGGTC ATGTGCGTTG CGATCGGCCA TTTCCGACAT TGAAGTAGAT 
AAGGTGGAAA TTCCTGGCAG GACCCTGCTG GCCATTCCGG GCTATACCGA AAAGGTTGAG 
TTTGGTGTGT TGGTGTCGTT TGCCTACAAA GTGGAAGGTA GCGACGAAGA GATCGTTGTG 
GCGACCACTC GTGTAGAGAC AATGCTTGGA GATACGGCCG TTGCCGTCCA TCCAAAGGAC 
GAACGCTACA AGCATCTACA CGGAAAGTTT GTAGTTCATC CGTTCTGCGA TCGTAAACTA 
CCGATTGTTT GCGATGATTT CGTTGAAATG GGCTTCGGAA CGGGCGCCGT CAAGATTACT 
CCAGCTCACG ATCCGAACGA TTATGAAGTT GGAAAACGTC ACAATTTGCC GTTCATTACC 
ATTTTCACGG ATGATGGTTT CATCACCGGG GAGTACGGAG AATTCACCGG AATGAAACGC 
TTCGATGCTA GGAAGGCTAT TCTGGTTGCT CTCCAGGCTA AGGGACTCTA CAAGGAAACC 
GTTGACAATC CGATGGTTGT GCCGATCTGT TCCCGCTCGA AGGATGTTGT GGAACCGCTG 
ATTAAGCCCC AGTGGTACGT CAAGTGTGAC GAGATGGCCA AGAACGCGAC GGAAGCGGTT 
CGCTCTGGAG AGCTGAAGAG CACGCCGGAA GTGCACAAGA AGAATTGGTA CCACTGGATG 
GATGAGATCC GCGATGGGTG TGTTTCTCGC CAACTTTGGG GGGGTCATAG AATCCCCGCC 
TATCAGGCTT CCTTCAAGGA CCCCAGTCAG AAGCCGGCGG GCCTTGACGC GGAATCATTA 
TGGGCCGTTG GGCGCACCGA GGTAGAAGCC TTGGAGAAGG CCGCGAAGGA GTTGAACGTT 
TCTAAAGAGG CTCGGGCTCT GAAGCAAGAC GAAGACGTCC GGGATACTTG GTTCAGCTCT 
GGTCTTTTCC CATTCTCTGT TGTCGGCTGG CCCGACAATA CCGTCGATGT GAAACTCTAC 
TACCCAACCT CCCTACTCGA GACGGGACAC GATATTTTGT TCTTCTGGGT GGCTGGCATG 
GTGTTCTTTG GCCAGACTTT GCTCGGCAAG CTTCCCTTCA AAGAAGTGTT CCTCCATCCG 
ATGGTCCGCG ATGCGCACGG CCGCAAAATG TCCAAATCGT TGGGTAATGT CATTGACCCG 
ATGGATGTGA TTACGGGAAT TTCCCTGGAC GGACTTCACC TGCAGCTGCT GGATTCGAAC 
CTGGACCCAA AGGAGATCGA GAAGGCGAAG GCCGGCCAGA AGCAGGACTA TCCTAACGGT 
ATTCCGGAAT GTGGAACCGA TGCTATGCGG TTCGCTCTGT GCGCCTACAA CACCCAGGGC 
CGGGACATTA ATTTGGACAT CATGCGCGTG CAGGGATATC GATTCTTCTG CAATAAGTTG 
TGGAACGCCA CTAAGTTCGC GCTGATGTAC TTCACCGGGG AAGAGCGCTA TGATGTGCTG 
CAAGAATTGA CTGGTGCCGA GAGCAACATT GACCGCTGGA TACTGTCCCG TCTGGCCAAC 
TGCATCGACG TTTCCAACAA GGGTTTCGAG AAGTACGAAT TTGCGCAAAC CACTAGCGCG 
TGCTACGAGT TCTGGCTGTA CGATCTGTGT GACGTCTATC TGGAATGTTT GAAACCCGTT 
TTCCAGTCCG GTTCGGAAGA AGCCAAGGCC TCTGCTCGGA AGACACTCTA TACCTGTTTG 
AATCTTGGCC TGAAACTGCT GTCGCCGTTT ATGCCGTTCA TTACCGAGGA ACTGTACCAG 
CGGCTGCCAC GTGCTGATGT CGAGCAGGTG GCCAGTATTT GCGTGGCTCC CTATCCGGAA 
GTGGAAAGCT CTAACTGGCA TAATGAACAA CTTGAGAAGG ACTTTGAATT TGTGCAACGC 
GTTGCAAAAG TTATCCGATC GGCCCGCAGC GATTACAACT TGCCGAACAA GACAAAAACC 
GAGGCGTTTG TTCTGTGCAG CGATGATGCC ACGAAGGATA CCCTGGAACG CTTTGCTGGG 
GATTTGACTA CCATGTCCTA CTCGCAGATT AGCCTCATCA GCGATGCTCC TCCAGCGGGA 
TGCGCTATTT TGACTGTGTC CGGAGCTTGT GTTGTTCATC TGCTTCTTAA GGGACTTATC 
GAGGTGGACA AGGAAGTGGA GAAACTTAAC AAGAAGAAAG ACAACTTGAG CTCAACCGTT 
GGCAAATTGG AACAGGCGAT GGGAGCGGCC GATTATGAAA CGAAGGTCCC CGAGGAAGTT 
CGGAACAACA ACCGGGAAAA GTTGGATCAG TCGAAAATCG AAATTGAACG TATCATTGCA 
GCAATAGAAA CCCTGAAGAT GATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL004327 ( XM_001648875.2 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001648925.2 Aedes aegypti valine--tRNA ligase (LOC5564593), mRNA.

Price & Availability↑

CloneID	OAe125085
Clone ID Related Accession (Same CDS sequence)	XM_001648875.2
Accession Version	XM_001648875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1500393600000
Organism	Aedes aegypti(yellow fever mosquito)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001648875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC CGGTGGAAAA TGGTACCGCT GCGGCCGAGT CCGGAACAGC TCCGGTCAAG 
ACGGAAAAGC AGCTCAAAAA GGAAGCGGAA AAGGCTGCCA AGTTGGCCAA GCTACAGGAG 
AAAATGAACA AAAAACAGCA GCAGGCGGAA CAGGCCGCCG TCAAGCCCAA AGCTGAGAAA 
AAAGCCAAGG AGACTGTGAA GGAGGCAATT GTATACAAAG GCAATACTCA GGAGGGCGAC 
AAGAAAGACC TGTCCGGTCC GTTCCCGGAA GCCTACAGTC CGCAGTACGT GGAAGCTGCC 
TGGTACAGCT GGTGGGAGAA GGAAGGATTC TTCAAGCCTG AATATGCGCG CAAGGAGAAC 
AATCCCAACG GGCAGTTTGT GATGGTGATT CCGCCGCCGA ATGTGACCGG TTCGTTGCAT 
TTGGGTCACG CACTGACGAA CGCCATCGAG GACTGCATCA CGCGTTGGAA CCGGATGAAG 
GGCCGCACCA CGTTGTGGGT TCCGGGATGT GATCATGCTG GAATTGCCAC CCAGGTCGTG 
GTGGAGAAGA AATTGTGGCG CGAGCAGAAG CAAACCCGAC ACGATTTGGG CCGGGAGAAG 
TTCATCGAAA AGATTTGGCA GTGGCGCAAT GAGAAGGGTG ATCGTATCTA TCATCAGCTG 
AAGAGGCTGG GATCGTCGTA CGACTGGTCC AGAGCTTGTT TTACGATGGA TCCCAAGCTG 
TGCAAGGCCG TGACGGAAGC TTTTGTTCGA TTGCATGAGC GCGGAATGAT CTACAGGAGC 
AACCGTTTGG GTAATTGGTC ATGTGCGTTG CGATCGGCCA TTTCCGACAT TGAAGTAGAT 
AAGGTGGAAA TTCCTGGCAG GACCCTGCTG GCCATTCCAG GCTATACCGA AAAGGTTGAG 
TTTGGTGTGT TGGTGTCGTT TGCCTACAAA GTGGAAGGTA GCGACGAAGA GATCGTTGTG 
GCGACCACTC GTGTAGAGAC AATGCTTGGA GATACGGCCG TTGCCGTGCA TCCAAAGGAC 
GAACGCTACA AGCATCTACA CGGAAAGTTT GTAGTTCATC CGTTCTGCGA TCGTAAACTA 
CCGATTGTTT GCGATGATTT CGTTGAAATG GGCTTCGGAA CGGGCGCCGT CAAGATTACT 
CCAGCTCACG ATCCGAACGA TTATGAAGTT GGAAAACGTC ACAATTTGCC GTTCATTACC 
ATTTTCACGG ATGATGGTTT CATCACCGGG GAGTACGGAG AATTCACCGG AATGAAACGC 
TTCGATGCTA GGAAGGCTAT TCTGGTTGCT CTCCAGGCTA AGGGACTCTA CAAGGAAACC 
GTTGATAATC CGATGGTTGT GCCGATCTGT TCCCGCTCGA AGGATGTTGT GGAACCGCTG 
ATTAAGCCCC AGTGGTACGT CAAGTGTGAC GAGATGGCCA AGAACGCTAC GGAAGCTGTT 
CGCTCTGGAG AGCTGAAGAT CACTCCGGAA GTGCACAAGA AGATTTGGTA CCACTGGATG 
GATGAGATCC GCGATTGGTG TGTTTCTCGC CAACTTTGGT GGGGTCATAG AATCCCCGCC 
TATCAGGCTT CCTTCAAGGA CCCCAGTCAG AAGCCGGCTG GCCTTGACGC GGAATCATTA 
TGGTTCGTTG GTCGCACCGA GGAAGAAGCC TTGGAGAAGG CCGCTAAGGA GTTGAACGTT 
TCTAAAGAGG CTCTTGCTCT GAAGCAAGAC GAAGACGTCC TGGATACTTG GTTCAGCTCT 
GGTCTTTTCC CATTCTCTGT TTTCGGCTGG CCCGACAATA CCGACGATCT GAAACTCTAC 
TACCCAACCT CCCTACTCGA GACGGGACAC GATATTTTGT TCTTCTGGGT GGCTCGCATG 
GTGTTCTTTG GCCAGACTTT GCTCGGCAAG CTTCCCTTCA AAGAAGTGTT CCTCCATCCG 
ATGGTCCGCG ATGCGCACGG CCGCAAAATG TCCAAATCGT TGGGTAATGT CATTGACCCG 
ATGGATGTGA TTACGGGAAT TTCCCTGGAC GGACTTCACC TGCAGCTGCT GGATTCGAAC 
CTGGACCCAA AGGAAATCGA GAAGGCGAAG GCCGGTCAGA AGCAGGACTA TCCTAACGGT 
ATTCCGGAAT GTGGAACCGA TGCTATGCGG TTCGCTCTGT GCGCCTACAA CACCCAGGGC 
CGGGACATTA ATTTGGACAT CATGCGCGTG CAGGGATATC GATTCTTCTG CAATAAGTTG 
TGGAACGCCA CTAAGTTCGC GCTGATGTAC TTCACCGGGG AAGAGCGCTA TGATGTGCTG 
CAAGAATTGA CTGGTGCCGA GAGCAACATT GACCGCTGGA TACTGTCCCG TCTGGCCAAC 
TGCATCGACG TTTCCAACAA GGGCTTCGAG AAGTACGAAT TTGCGCAAAC CACTAGCGCG 
TGCTACGAGT TCTGGCTGTA CGATCTGTGT GACGTCTATC TGGAATGTTT GAAACCCGTT 
TTCCAGTCCG GTTCGGAAGA AGCCAAGGCC TCTGCTCGGA AGACACTCTA TACCTGTTTG 
AATCTTGGCC TGAAACTGCT GTCGCCGTTT ATGCCGTTCA TTACCGAGGA ACTGTACCAG 
CGGCTGCCAC GTGCTGATGT CGAGCAGGTG GCCAGTATTT GCGTGGCTCC CTATCCGGAA 
GTGGAAAGCT CTAACTGGCA TAATGAACAA CTTGAGAAGG ACTTTGAATT TGTGCAACGC 
GTTGCAAAAG TTATCCGATC GGCCCGCAGC GATTACAACT TGCCGAACAA GACAAAAACC 
GAGGCGTTTG TTCTGTGCAG CGATGATGCC ACGAAGGACA CCCTGGAACG CTTTGCTGGG 
GATTTGACTA CCATGTCCTA CTCGCAGATT AGCCTCATCA GCGATGCTCC TCCAGCGGGA 
TGCGCTATTT TGACTGTGTC CGGTGCTTGT GTTGTTCATC TGCTACTTAA GGGACTTATC 
GAGGTGGACA AGGAAGTGGA GAAACTTAAC AAGAAGAAAG ACAACTTGAG CTCAACCGTT 
GGCAAGCTGG AACAGGCGAT GGGAGCGGCC GATTATGAAA CGAAGGTCCC CGAGGAAGTT 
CGGAACAACA ACCGGGAAAA GTTGGATCAG TCGAAAATCG AAATTGAACG TATCATTGCA 
GCAATAGAAA CCCTGAAGAT GATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001648925.2
CDS	241..3387
Translation	MSDPVENGTAAAESGTAPVKTEKQLKKEAEKAAKLAKLQEKMNKKQQQAEQAAVKPKAEKKAKETVKEAIVYKGNTQEGDKKDLSGPFPEAYSPQYVEAAWYSWWEKEGFFKPEYARKENNPNGQFVMVIPPPNVTGSLHLGHALTNAIEDCITRWNRMKGRTTLWVPGCDHAGIATQVVVEKKLWREQKQTRHDLGREKFIEKIWQWRNEKGDRIYHQLKRLGSSYDWSRACFTMDPKLCKAVTEAFVRLHERGMIYRSNRLGNWSCALRSAISDIEVDKVEIPGRTLLAIPGYTEKVEFGVLVSFAYKVEGSDEEIVVATTRVETMLGDTAVAVHPKDERYKHLHGKFVVHPFCDRKLPIVCDDFVEMGFGTGAVKITPAHDPNDYEVGKRHNLPFITIFTDDGFITGEYGEFTGMKRFDARKAILVALQAKGLYKETVDNPMVVPICSRSKDVVEPLIKPQWYVKCDEMAKNATEAVRSGELKITPEVHKKIWYHWMDEIRDWCVSRQLWWGHRIPAYQASFKDPSQKPAGLDAESLWFVGRTEEEALEKAAKELNVSKEALALKQDEDVLDTWFSSGLFPFSVFGWPDNTDDLKLYYPTSLLETGHDILFFWVARMVFFGQTLLGKLPFKEVFLHPMVRDAHGRKMSKSLGNVIDPMDVITGISLDGLHLQLLDSNLDPKEIEKAKAGQKQDYPNGIPECGTDAMRFALCAYNTQGRDINLDIMRVQGYRFFCNKLWNATKFALMYFTGEERYDVLQELTGAESNIDRWILSRLANCIDVSNKGFEKYEFAQTTSACYEFWLYDLCDVYLECLKPVFQSGSEEAKASARKTLYTCLNLGLKLLSPFMPFITEELYQRLPRADVEQVASICVAPYPEVESSNWHNEQLEKDFEFVQRVAKVIRSARSDYNLPNKTKTEAFVLCSDDATKDTLERFAGDLTTMSYSQISLISDAPPAGCAILTVSGACVVHLLLKGLIEVDKEVEKLNKKKDNLSSTVGKLEQAMGAADYETKVPEEVRNNNREKLDQSKIEIERIIAAIETLKMM

Target ORF information:

RefSeq Version	XM_001648875.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti valine--tRNA ligase (LOC5564593), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001648875.2

ORF Insert Sequence:

ATGTCCGATC CGGTGGAAAA TGGTACCGCT GCGGCCGAGT CCGGAACAGC TCCGGTCAAG 
ACGGAAAAGC AGCTCAAAAA GGAAGCGGAA AAGGCTGCCA AGTTGGCCAA GCTACAGGAG 
AAAATGAACA AAAAACAGCA GCAGGCGGAA CAGGCCGCCG TCAAGCCCAA AGCTGAGAAA 
AAAGCCAAGG AGACTGTGAA GGAGGCAATT GTATACAAAG GCAATACTCA GGAGGGCGAC 
AAGAAAGACC TGTCCGGTCC GTTCCCGGAA GCCTACAGTC CGCAGTACGT GGAAGCTGCC 
TGGTACAGCT GGTGGGAGAA GGAAGGATTC TTCAAGCCTG AATATGCGCG CAAGGAGAAC 
AATCCCAACG GGCAGTTTGT GATGGTGATT CCGCCGCCGA ATGTGACCGG TTCGTTGCAT 
TTGGGTCACG CACTGACGAA CGCCATCGAG GACTGCATCA CGCGTTGGAA CCGGATGAAG 
GGCCGCACCA CGTTGTGGGT TCCGGGATGT GATCATGCTG GAATTGCCAC CCAGGTCGTG 
GTGGAGAAGA AATTGTGGCG CGAGCAGAAG CAAACCCGAC ACGATTTGGG CCGGGAGAAG 
TTCATCGAAA AGATTTGGCA GTGGCGCAAT GAGAAGGGTG ATCGTATCTA TCATCAGCTG 
AAGAGGCTGG GATCGTCGTA CGACTGGTCC AGAGCTTGTT TTACGATGGA TCCCAAGCTG 
TGCAAGGCCG TGACGGAAGC TTTTGTTCGA TTGCATGAGC GCGGAATGAT CTACAGGAGC 
AACCGTTTGG GTAATTGGTC ATGTGCGTTG CGATCGGCCA TTTCCGACAT TGAAGTAGAT 
AAGGTGGAAA TTCCTGGCAG GACCCTGCTG GCCATTCCAG GCTATACCGA AAAGGTTGAG 
TTTGGTGTGT TGGTGTCGTT TGCCTACAAA GTGGAAGGTA GCGACGAAGA GATCGTTGTG 
GCGACCACTC GTGTAGAGAC AATGCTTGGA GATACGGCCG TTGCCGTGCA TCCAAAGGAC 
GAACGCTACA AGCATCTACA CGGAAAGTTT GTAGTTCATC CGTTCTGCGA TCGTAAACTA 
CCGATTGTTT GCGATGATTT CGTTGAAATG GGCTTCGGAA CGGGCGCCGT CAAGATTACT 
CCAGCTCACG ATCCGAACGA TTATGAAGTT GGAAAACGTC ACAATTTGCC GTTCATTACC 
ATTTTCACGG ATGATGGTTT CATCACCGGG GAGTACGGAG AATTCACCGG AATGAAACGC 
TTCGATGCTA GGAAGGCTAT TCTGGTTGCT CTCCAGGCTA AGGGACTCTA CAAGGAAACC 
GTTGATAATC CGATGGTTGT GCCGATCTGT TCCCGCTCGA AGGATGTTGT GGAACCGCTG 
ATTAAGCCCC AGTGGTACGT CAAGTGTGAC GAGATGGCCA AGAACGCTAC GGAAGCTGTT 
CGCTCTGGAG AGCTGAAGAT CACTCCGGAA GTGCACAAGA AGATTTGGTA CCACTGGATG 
GATGAGATCC GCGATTGGTG TGTTTCTCGC CAACTTTGGT GGGGTCATAG AATCCCCGCC 
TATCAGGCTT CCTTCAAGGA CCCCAGTCAG AAGCCGGCTG GCCTTGACGC GGAATCATTA 
TGGTTCGTTG GTCGCACCGA GGAAGAAGCC TTGGAGAAGG CCGCTAAGGA GTTGAACGTT 
TCTAAAGAGG CTCTTGCTCT GAAGCAAGAC GAAGACGTCC TGGATACTTG GTTCAGCTCT 
GGTCTTTTCC CATTCTCTGT TTTCGGCTGG CCCGACAATA CCGACGATCT GAAACTCTAC 
TACCCAACCT CCCTACTCGA GACGGGACAC GATATTTTGT TCTTCTGGGT GGCTCGCATG 
GTGTTCTTTG GCCAGACTTT GCTCGGCAAG CTTCCCTTCA AAGAAGTGTT CCTCCATCCG 
ATGGTCCGCG ATGCGCACGG CCGCAAAATG TCCAAATCGT TGGGTAATGT CATTGACCCG 
ATGGATGTGA TTACGGGAAT TTCCCTGGAC GGACTTCACC TGCAGCTGCT GGATTCGAAC 
CTGGACCCAA AGGAAATCGA GAAGGCGAAG GCCGGTCAGA AGCAGGACTA TCCTAACGGT 
ATTCCGGAAT GTGGAACCGA TGCTATGCGG TTCGCTCTGT GCGCCTACAA CACCCAGGGC 
CGGGACATTA ATTTGGACAT CATGCGCGTG CAGGGATATC GATTCTTCTG CAATAAGTTG 
TGGAACGCCA CTAAGTTCGC GCTGATGTAC TTCACCGGGG AAGAGCGCTA TGATGTGCTG 
CAAGAATTGA CTGGTGCCGA GAGCAACATT GACCGCTGGA TACTGTCCCG TCTGGCCAAC 
TGCATCGACG TTTCCAACAA GGGCTTCGAG AAGTACGAAT TTGCGCAAAC CACTAGCGCG 
TGCTACGAGT TCTGGCTGTA CGATCTGTGT GACGTCTATC TGGAATGTTT GAAACCCGTT 
TTCCAGTCCG GTTCGGAAGA AGCCAAGGCC TCTGCTCGGA AGACACTCTA TACCTGTTTG 
AATCTTGGCC TGAAACTGCT GTCGCCGTTT ATGCCGTTCA TTACCGAGGA ACTGTACCAG 
CGGCTGCCAC GTGCTGATGT CGAGCAGGTG GCCAGTATTT GCGTGGCTCC CTATCCGGAA 
GTGGAAAGCT CTAACTGGCA TAATGAACAA CTTGAGAAGG ACTTTGAATT TGTGCAACGC 
GTTGCAAAAG TTATCCGATC GGCCCGCAGC GATTACAACT TGCCGAACAA GACAAAAACC 
GAGGCGTTTG TTCTGTGCAG CGATGATGCC ACGAAGGACA CCCTGGAACG CTTTGCTGGG 
GATTTGACTA CCATGTCCTA CTCGCAGATT AGCCTCATCA GCGATGCTCC TCCAGCGGGA 
TGCGCTATTT TGACTGTGTC CGGTGCTTGT GTTGTTCATC TGCTACTTAA GGGACTTATC 
GAGGTGGACA AGGAAGTGGA GAAACTTAAC AAGAAGAAAG ACAACTTGAG CTCAACCGTT 
GGCAAGCTGG AACAGGCGAT GGGAGCGGCC GATTATGAAA CGAAGGTCCC CGAGGAAGTT 
CGGAACAACA ACCGGGAAAA GTTGGATCAG TCGAAAATCG AAATTGAACG TATCATTGCA 
GCAATAGAAA CCCTGAAGAT GATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL004327 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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