ORF » Species Summary » Schizosaccharomyces pombe » top1 cDNA ORF clone

top1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following top1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc101577 NM_001022128.2
Latest version!		 Schizosaccharomyces pombe DNA topoisomerase I (top1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    top1 ( NM_001022128.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596209.1 Schizosaccharomyces pombe DNA topoisomerase I (top1), mRNA.
    Price & Availability↑
    CloneID OSc101577
    Clone ID Related Accession (Same CDS sequence) NM_001022128.2
    Accession Version NM_001022128.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1522598400000
    Organism Schizosaccharomyces pombe(fission yeast)
    Product DNA topoisomerase I
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022128.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCTTCGT CTGATTCAGA TTCCGTGTCT TTATCCATTA GAAGGAGGCA AAGACGCGGT 
    TCATCTAAAC GGATTAGTAT GAAGGAGTCG GACGAAGAGA GTGATAGTTC TGAAAATCAT 
    CCTCTTAGTG AATCGTTGAA TAAGAAATCC AAATCCGAGA GTGATGAGGA TGATATACCG 
    ATTAGAAAGA GACGTGCTTC TTCAAAGAAA AATATGTCCA ATTCAAGCTC TAAAAAAAGA 
    GCTAAAGTAA TGGGAAATGG TGGTTTAAAA AACGGAAAAA AAACGGCGGT TGTAAAAGAG 
    GAAGAGGATT TCAATGAAAT TGCAAAGCCC TCACCGAAGC ATAAACGTGT TTCTAAAGCG 
    AATGGAAGTA AAAATGGCGC GAAGTCAGCT GTTAAAAAAG AGGAAAGTGA CACAGATGAT 
    TCAGTTCCAT TGAGAGCTGT TTCGACTGTA TCTTTAACTC CTTACAAATC AGAACTGCCT 
    TCGGGAGCTT CGACCACGCA AAATCGCTCA CCAAACGATG AGGAAGATGA AGATGAGGAT 
    TACAAGTGGT GGACTTCAGA AAATATCGAT GATACTCAAA AATGGACTAC ATTGGAGCAT 
    AATGGTGTAA TTTTTGCTCC ACCCTATGAA CCTTTACCAA AGAACGTCAA GCTAATTTAC 
    GATGGAAACC CCGTAAATCT TCCTCCCGAA GCAGAAGAAG TTGCTGGTTT TTATGCTGCA 
    ATGCTTGAAA CCGATCATGC CAAAAATCCT GTATTCCAAG ATAATTTTTT CCGTGACTTC 
    TTAAAGGTCT GTGATGAATG TAACTTTAAT CACAACATTA AAGAGTTTTC TAAATGTGAT 
    TTTACCCAAA TGTTTCACCA TTTTGAGCAA AAGAGGGAAG AGAAGAAGAG TATGCCGAAG 
    GAACAGAAGA AGGCAATAAA GCAGAAAAAA GATGAGGAAG AGGAAAAATA TAAATGGTGC 
    ATACTTGATG GGAGAAAGGA GAAGGTTGGT AACTTTCGTA TTGAACCTCC AGGGTTATTT 
    CGTGGTCGAG GTAGTCATCC TAAAACTGGT TCTTTAAAGC GTCGAGTATA TCCTGAACAA 
    ATTACCATTA ATATTGGTGA AGGTGTACCC GTTCCAGAAC CACTCCCTGG GCATCAATGG 
    GCGGAGGTAA AGCATGATAA TACAGTGACC TGGTTGGCAA CCTGGCATGA AAATATAAAT 
    AATAATGTCA AATATGTCTT TTTAGCTGCA GGAAGTTCTC TAAAAGGACA GAGTGACTTA 
    AAAAAATACG AAAAGTCAAG AAAGCTTAAG GATTATATTG ATGATATCCG TAAAGGCTAC 
    CGGAAAGATT TGAAAAGTGA GTTAACGGTT GAGCGTCAAA GAGGAACTGC CATGTATTTA 
    ATTGATGTTT TTGCTTTAAG AGCAGGAAAT GAAAAGGGTG AAGACGAGGC GGATACTGTT 
    GGTTGTTGTT CACTGCGATA TGAACATGTT ACACTGAAGC CACCACGAAC AGTCGTTTTC 
    GATTTTCTTG GTAAAGATTC TATTCGTTAC TACAACGAGG TTGAAGTTGA TCCCCAAGTT 
    TTTAAAAATC TAAAGATCTT TAAACGTCCT CCCAAAAAAG AGGGTGATTT AATTTTCGAC 
    CGTCTCAGTA CAAACAGTCT TAACAAATAT CTGACTAGCC TTATGGATGG ACTTTCAGCT 
    AAAGTATTTC GTACCTACAA TGCTTCATAC ACCATGGCCG AGGAACTTAA GAAAATGCCT 
    AAGAACCTCA CCCTTGCAGA CAAAATACTA TTTTATAATA GGGCAAATAG GACTGTTGCA 
    ATTTTATGTA ATCATCAACG TTCCGTAACC AAAAATCACG ATGTTCAAAT GGAACGGTTT 
    GCCGAAAGGA TTAAGGCATT ACAATACCAG CGGATGAGAC TGCGAAAAAT GATGCTGAAT 
    TTAGAGCCCA AGCTTGCTAA AAGCAAGCCC GAATTGCTGG CTAAAGAAGA AGGCATTACC 
    GATTCATGGA TCGTAAAACA TCACGAGACA CTTTACGAAC TAGAAAAAGA GAAAATAAAA 
    AAGAAATTCG ATCGTGAGAA CGAAAAATTA GCTGCTGAGG ATCCCAAATC AGTGCTTCCG 
    GAATCTGAAT TGGAAGTTCG ATTGAAAGCG GCTGATGAGT TGAAGAAAGC GCTGGACGCT 
    GAACTTAAAA GCAAAAAAGT CGATCCAGGT CGTTCTTCGA TGGAACAACT TGAGAAAAGA 
    TTAAACAAAC TCAATGAACG AATTAATGTT ATGCGTACTC AGATGATCGA TAAAGACGAG 
    AATAAAACTA CTGCTTTGGG TACAAGTAAG ATTAACTACA TAGACCCGAG GCTTACTTAT 
    TCGTTCAGCA AGCGAGAAGA CGTTCCTATT GAGAAGCTGT TTAGTAAGAC GATTCGTGAC 
    AAGTTCAATT GGGCTGCTGA TACACCTCCG GATTGGAAGT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596209.1
    CDS14..2458
    Translation

    Target ORF information:

    RefSeq Version NM_001022128.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe DNA topoisomerase I (top1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001022128.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCTTCGT CTGATTCAGA TTCCGTGTCT TTATCCATTA GAAGGAGGCA AAGACGCGGT 
    TCATCTAAAC GGATTAGTAT GAAGGAGTCG GACGAAGAGA GTGATAGTTC TGAAAATCAT 
    CCTCTTAGTG AATCGTTGAA TAAGAAATCC AAATCCGAGA GTGATGAGGA TGATATACCG 
    ATTAGAAAGA GACGTGCTTC TTCAAAGAAA AATATGTCCA ATTCAAGCTC TAAAAAAAGA 
    GCTAAAGTAA TGGGAAATGG TGGTTTAAAA AACGGAAAAA AAACGGCGGT TGTAAAAGAG 
    GAAGAGGATT TCAATGAAAT TGCAAAGCCC TCACCGAAGC ATAAACGTGT TTCTAAAGCG 
    AATGGAAGTA AAAATGGCGC GAAGTCAGCT GTTAAAAAAG AGGAAAGTGA CACAGATGAT 
    TCAGTTCCAT TGAGAGCTGT TTCGACTGTA TCTTTAACTC CTTACAAATC AGAACTGCCT 
    TCGGGAGCTT CGACCACGCA AAATCGCTCA CCAAACGATG AGGAAGATGA AGATGAGGAT 
    TACAAGTGGT GGACTTCAGA AAATATCGAT GATACTCAAA AATGGACTAC ATTGGAGCAT 
    AATGGTGTAA TTTTTGCTCC ACCCTATGAA CCTTTACCAA AGAACGTCAA GCTAATTTAC 
    GATGGAAACC CCGTAAATCT TCCTCCCGAA GCAGAAGAAG TTGCTGGTTT TTATGCTGCA 
    ATGCTTGAAA CCGATCATGC CAAAAATCCT GTATTCCAAG ATAATTTTTT CCGTGACTTC 
    TTAAAGGTCT GTGATGAATG TAACTTTAAT CACAACATTA AAGAGTTTTC TAAATGTGAT 
    TTTACCCAAA TGTTTCACCA TTTTGAGCAA AAGAGGGAAG AGAAGAAGAG TATGCCGAAG 
    GAACAGAAGA AGGCAATAAA GCAGAAAAAA GATGAGGAAG AGGAAAAATA TAAATGGTGC 
    ATACTTGATG GGAGAAAGGA GAAGGTTGGT AACTTTCGTA TTGAACCTCC AGGGTTATTT 
    CGTGGTCGAG GTAGTCATCC TAAAACTGGT TCTTTAAAGC GTCGAGTATA TCCTGAACAA 
    ATTACCATTA ATATTGGTGA AGGTGTACCC GTTCCAGAAC CACTCCCTGG GCATCAATGG 
    GCGGAGGTAA AGCATGATAA TACAGTGACC TGGTTGGCAA CCTGGCATGA AAATATAAAT 
    AATAATGTCA AATATGTCTT TTTAGCTGCA GGAAGTTCTC TAAAAGGACA GAGTGACTTA 
    AAAAAATACG AAAAGTCAAG AAAGCTTAAG GATTATATTG ATGATATCCG TAAAGGCTAC 
    CGGAAAGATT TGAAAAGTGA GTTAACGGTT GAGCGTCAAA GAGGAACTGC CATGTATTTA 
    ATTGATGTTT TTGCTTTAAG AGCAGGAAAT GAAAAGGGTG AAGACGAGGC GGATACTGTT 
    GGTTGTTGTT CACTGCGATA TGAACATGTT ACACTGAAGC CACCACGAAC AGTCGTTTTC 
    GATTTTCTTG GTAAAGATTC TATTCGTTAC TACAACGAGG TTGAAGTTGA TCCCCAAGTT 
    TTTAAAAATC TAAAGATCTT TAAACGTCCT CCCAAAAAAG AGGGTGATTT AATTTTCGAC 
    CGTCTCAGTA CAAACAGTCT TAACAAATAT CTGACTAGCC TTATGGATGG ACTTTCAGCT 
    AAAGTATTTC GTACCTACAA TGCTTCATAC ACCATGGCCG AGGAACTTAA GAAAATGCCT 
    AAGAACCTCA CCCTTGCAGA CAAAATACTA TTTTATAATA GGGCAAATAG GACTGTTGCA 
    ATTTTATGTA ATCATCAACG TTCCGTAACC AAAAATCACG ATGTTCAAAT GGAACGGTTT 
    GCCGAAAGGA TTAAGGCATT ACAATACCAG CGGATGAGAC TGCGAAAAAT GATGCTGAAT 
    TTAGAGCCCA AGCTTGCTAA AAGCAAGCCC GAATTGCTGG CTAAAGAAGA AGGCATTACC 
    GATTCATGGA TCGTAAAACA TCACGAGACA CTTTACGAAC TAGAAAAAGA GAAAATAAAA 
    AAGAAATTCG ATCGTGAGAA CGAAAAATTA GCTGCTGAGG ATCCCAAATC AGTGCTTCCG 
    GAATCTGAAT TGGAAGTTCG ATTGAAAGCG GCTGATGAGT TGAAGAAAGC GCTGGACGCT 
    GAACTTAAAA GCAAAAAAGT CGATCCAGGT CGTTCTTCGA TGGAACAACT TGAGAAAAGA 
    TTAAACAAAC TCAATGAACG AATTAATGTT ATGCGTACTC AGATGATCGA TAAAGACGAG 
    AATAAAACTA CTGCTTTGGG TACAAGTAAG ATTAACTACA TAGACCCGAG GCTTACTTAT 
    TCGTTCAGCA AGCGAGAAGA CGTTCCTATT GAGAAGCTGT TTAGTAAGAC GATTCGTGAC 
    AAGTTCAATT GGGCTGCTGA TACACCTCCG GATTGGAAGT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Schäfer M,Müller-Auer S,Gabel C,Fuchs M,Düsterhöft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dréano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,Sánchez M,del Rey F,Benito J,Domínguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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