Dpse\GA20127 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA20127
Entrez Gene ID	4817482
Full Name	GA20127 gene product from transcript GA20127-RB
Synonyms	GA20127,dpse_GLEANR_2117
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001356442.3	XP_001356478.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA20127 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA20127 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe17404	XM_001356442.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20127), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA20127 ( XM_001356442.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001356478.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20127), mRNA.

Price & Availability↑

CloneID	ODe17404
Clone ID Related Accession (Same CDS sequence)	XM_001356442.3
Accession Version	XM_001356442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589964). On Dec 22, 2015 this sequence version replaced XM_001356442.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCTGG ACGATGACAA CTCAGATCTC TTGGTCTGTG CATCCGAAAT GATGAAAGAC 
CGTTTGTATT TTGTGGCGTT CAAGAAGAAC ATCAAGCCCA AGAATACCGT CAACACGCAC 
TACTTCAGCA TCGATGAGGA GTTCATCTAC GAGAACTTTT ACAGCGATTT TGGTCCCCTC 
AATATCTGCA TGCTGTATCG ATACTGCATG AAGCTCAACA CGAAGCTGAG TGCCAAGTGC 
CATGCCAACA GAAAGATTGT CCACTACACC TCCATGAATC CGGCCAAGCG GCTCAATGCG 
GCATATCTGA TCGGTTCGTA TGCCATTATC TACCTGAATA AGACGCCTCT GGAGGCCTAT 
AGGCCTCTGG TGTCTGGCGA AATACCCGCC TATACGCGCT TCTGCGATGC CAGCTATGGT 
CCCAGCAGTT TCAAGATCTC CCTGCTGGAT TGCCTGAATG CCGTGCACAA GGGCCTGCAG 
GCGGGCTTCT TCAATTTCGA TGACTTTGAT GCCGAGGAGT ACGAGTACTT TGAGCGCGTG 
GAGAACGGCG ACTTCAATTG GATTGTCCCC CAGAAGTTTA TTGCCTTTTG TGGGCCACAT 
CAGAAGAGCA AGACCCTGCC CAATGGGTAT CCGTGCCATG CACCGGAGCG ATACTTTTCC 
TACTTTCGGG AGAACCATGT GACCACCGTT ATTCGACTGA ATGCGAAGGT CTACCATGCC 
TCCTCGTTTG AGAATGCCGG CTTCGATCAC AAGGATCTGT TCTTCATCGA TGGAAGCACA 
CCAAGCGATG CCATCATGAA GAAGTTCCTC TCCATCTGTG AGACCACAAA GGGTGCCATA 
GCCGTGCATT GCAAGGCGGG ACTAGGTCGC ACGGGCAGCC TCATTGGAGC GTATATGATG 
AAGCATTATG GTTTTACCGC ACTTGAGGCC ATTGCCTGGC TAAGGCTGTG CCGCCCGGGT 
TCCGTGATTG GCCATCAGCA GCAGTGGATG GAGGACAAAC AAAGCTGGTT GTGGTCCGAG 
GGCGAACGTA TGCGCCGTCG CACTGGTGTT CCCATGCTAC GGCACACGTA TGGCATCTAC 
AGCCTGGAGC TGAAGACCAA ACTGACGGAA GCCTCCGCCG CCTCGAATAC CCCCGTGGAT 
CTGCTGATGA ATCGAGTCAA GGGGATTTCC CAACGTGTGG ACACAATGCA TCTGAATGAT 
CAGGACACAG CCCTGGATTC GGGTGCCGAC CAGACGGACG AAGAGATCTC CGAGCAGCGG 
CGCCTAGAGC GGAACGAACG AGCGCTGTAC CAGCGCTGCA CCACGGAAGA TCCCAATTGC 
AATGTCAGCG ACAACGACAC GGATACGGCC AGCGCACCGG CCGAAGCCCC ACCGCTGGCC 
GCCACCTACA CAATGTCAAC GCGACGCCGC AAGTCCCCGT CGGCGGCCAA CAAACCCACA 
GTGATTGCCA ACTCCCTGAG GCGCCTGGTC AATCCGGTGG TCACCCATCG TAGTGTGCAC 
AGCGCCACCC CACTGATGAC AACTCCGGGT GTTGTTTATG CCACGCCCGA CGTCACCACC 
TGCACTGAGA AGAAGCTGCG GAAGCCCAGT GGCAATGTAT TCGATGCGGC CCGCCATACC 
ATCGCATCGA CTGTGCGGAT GACGCAGACG GCGCTGGAGA AGAAACAGGC CCAGACGCAG 
GGCGACAAAC TGAATCAGAT CAAGGCGCTG CGACGGCACC ATTCGCGCTC CGTAAATGTT 
AACAGCAACA ACGAGCTGGA CTCGAATCTG AGGCATACGC GAGCCCGTTC TCAGCCCTTC 
CGGAACAACA ACAATGGGGT GACGGCGACA ACCCACGCGA CGATAACCGC GAGATGTGCA 
ACGCAGACCA CGCCCTCCCT GCTGCAAGGA ACAGTGCCGG GAGTGTCGGC GGCAGCGGTG 
GCGGTTGCTT TGGCCAACAA TCTGAACAAT AACACCAGCA ATAGTACCCT CTCTCACAGC 
AGTACTCTAA ACCCATCCCT GAATAACCAG AATTATAATC AGAACAAAAA CAACAATGAT 
GGCACCACTA ACAATCTCCT GCCCGGCAGT CGCACCCGCA GCCTGGCGAT CTACAGCAAG 
AGAATGGAAT TGAAACACCT GCGCAAGGCG GAACAGCAGG AGCAGCCGGT ATCCACTACA 
ACGAACAGCG TCAAGCCGCC ACCGTATGCC ACCATCAACG AGAGCAAGAT CCCTGTGGTG 
ATGATGAGCA GTGCTGCCGC TGGCAGCTCG GCCACCATTC CCCCCACGCG CGGCAGTGCC 
GCCCGCACCT CGCACCACCA CATGACTCTT CGCGGTCGCT CGCGTATCCG CTATCAGCGT 
CCAGCGGCCG GAGAGCCCGC GCCTGCGGCC GCTCAGGCAC AGCCCTGCAC GGTGGCGACG 
GCGCGCTACT ATCGCGATGT GTCCGCCATA CCCACCGCCA TGTCGTCGTT GATTGCTTTG 
GGAACTAGTT CTTCCAACTC TGCTTCCACT TGTACTACTT CTACTGCTGG CTCTGGTTGC 
TATAATATAG CCACACGATC CTCCTCCGCT ACGCTCGATC TGAACTCGAA TCCATTGCCA 
GCGGCCACAC AAGGCACTGG GAGTGGGACT GGGGCTGGGG CCAAGTCCAA GCTATCCAAC 
AACACGAACA ACAACCTCAA CAACAACTGC AATTTGAGTA ATGCCCATTC TTCTGTTTCT 
CCTCCACCTA ACCACAACCA CAACCACCAC CTCCCATCAT CCTCTCCAGA CACAGGCCGT 
CTTAGTGGGC GCGGCAGTGC CGAACTGGAG GCAATCATCG AGCACGTGGG AGCGAGGCGG 
AACAAGCGAT CTCTGAGCTC CACGCGCCTG GACAAGGACA AGTGCGACGA ATACAACACG 
AAACTACTGC GTAAGACGAG CATCGTAGCA GCGGCCGCCG TAGCAGCAGC AGCCTCCGCA 
CCCACTCCTG CCAACCACAA TCCGAACAAC AACAACCATA GTGGAGGCAG CAGCAAATAC 
AGCAGCAACA GTGGCACACC CACAACACCC AGCACTGGAG GCAGCTTCAA GTTGTCGCGT 
CGGGTGTTTG GTGAATCGGG TTCCTCGGCA TCGACATCGA CCTCCAACTC GGGCATACCC 
ACGCCAACGG CACCGACACC GTCATCCACG CAGCAGTGGC AGCAGCACCA TGTGGCACCA 
CGTGTTGGTA GCCGCCTGTC CAGCGATCGG GAGCGAGAGC GCCAACAGCA GGAGCAGCAA 
CAGTCGCTGA AGCTGCGCAA GAAGATCAGC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001356478.3
CDS	662..3937
Translation	MMLDDDNSDLLVCASEMMKDRLYFVAFKKNIKPKNTVNTHYFSIDEEFIYENFYSDFGPLNICMLYRYCMKLNTKLSAKCHANRKIVHYTSMNPAKRLNAAYLIGSYAIIYLNKTPLEAYRPLVSGEIPAYTRFCDASYGPSSFKISLLDCLNAVHKGLQAGFFNFDDFDAEEYEYFERVENGDFNWIVPQKFIAFCGPHQKSKTLPNGYPCHAPERYFSYFRENHVTTVIRLNAKVYHASSFENAGFDHKDLFFIDGSTPSDAIMKKFLSICETTKGAIAVHCKAGLGRTGSLIGAYMMKHYGFTALEAIAWLRLCRPGSVIGHQQQWMEDKQSWLWSEGERMRRRTGVPMLRHTYGIYSLELKTKLTEASAASNTPVDLLMNRVKGISQRVDTMHLNDQDTALDSGADQTDEEISEQRRLERNERALYQRCTTEDPNCNVSDNDTDTASAPAEAPPLAATYTMSTRRRKSPSAANKPTVIANSLRRLVNPVVTHRSVHSATPLMTTPGVVYATPDVTTCTEKKLRKPSGNVFDAARHTIASTVRMTQTALEKKQAQTQGDKLNQIKALRRHHSRSVNVNSNNELDSNLRHTRARSQPFRNNNNGVTATTHATITARCATQTTPSLLQGTVPGVSAAAVAVALANNLNNNTSNSTLSHSSTLNPSLNNQNYNQNKNNNDGTTNNLLPGSRTRSLAIYSKRMELKHLRKAEQQEQPVSTTTNSVKPPPYATINESKIPVVMMSSAAAGSSATIPPTRGSAARTSHHHMTLRGRSRIRYQRPAAGEPAPAAAQAQPCTVATARYYRDVSAIPTAMSSLIALGTSSSNSASTCTTSTAGSGCYNIATRSSSATLDLNSNPLPAATQGTGSGTGAGAKSKLSNNTNNNLNNNCNLSNAHSSVSPPPNHNHNHHLPSSSPDTGRLSGRGSAELEAIIEHVGARRNKRSLSSTRLDKDKCDEYNTKLLRKTSIVAAAAVAAAASAPTPANHNPNNNNHSGGSSKYSSNSGTPTTPSTGGSFKLSRRVFGESGSSASTSTSNSGIPTPTAPTPSSTQQWQQHHVAPRVGSRLSSDRERERQQQEQQQSLKLRKKISY

Target ORF information:

RefSeq Version	XM_001356442.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20127), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001356442.3

ORF Insert Sequence:

ATGATGCTGG ACGATGACAA CTCAGATCTC TTGGTCTGTG CATCCGAAAT GATGAAAGAC 
CGTTTGTATT TTGTGGCGTT CAAGAAGAAC ATCAAGCCCA AGAATACCGT CAACACGCAC 
TACTTCAGCA TCGATGAGGA GTTCATCTAC GAGAACTTTT ACAGCGATTT TGGTCCCCTC 
AATATCTGCA TGCTGTATCG ATACTGCATG AAGCTCAACA CGAAGCTGAG TGCCAAGTGC 
CATGCCAACA GAAAGATTGT CCACTACACC TCCATGAATC CGGCCAAGCG GCTCAATGCG 
GCATATCTGA TCGGTTCGTA TGCCATTATC TACCTGAATA AGACGCCTCT GGAGGCCTAT 
AGGCCTCTGG TGTCTGGCGA AATACCCGCC TATACGCGCT TCTGCGATGC CAGCTATGGT 
CCCAGCAGTT TCAAGATCTC CCTGCTGGAT TGCCTGAATG CCGTGCACAA GGGCCTGCAG 
GCGGGCTTCT TCAATTTCGA TGACTTTGAT GCCGAGGAGT ACGAGTACTT TGAGCGCGTG 
GAGAACGGCG ACTTCAATTG GATTGTCCCC CAGAAGTTTA TTGCCTTTTG TGGGCCACAT 
CAGAAGAGCA AGACCCTGCC CAATGGGTAT CCGTGCCATG CACCGGAGCG ATACTTTTCC 
TACTTTCGGG AGAACCATGT GACCACCGTT ATTCGACTGA ATGCGAAGGT CTACCATGCC 
TCCTCGTTTG AGAATGCCGG CTTCGATCAC AAGGATCTGT TCTTCATCGA TGGAAGCACA 
CCAAGCGATG CCATCATGAA GAAGTTCCTC TCCATCTGTG AGACCACAAA GGGTGCCATA 
GCCGTGCATT GCAAGGCGGG ACTAGGTCGC ACGGGCAGCC TCATTGGAGC GTATATGATG 
AAGCATTATG GTTTTACCGC ACTTGAGGCC ATTGCCTGGC TAAGGCTGTG CCGCCCGGGT 
TCCGTGATTG GCCATCAGCA GCAGTGGATG GAGGACAAAC AAAGCTGGTT GTGGTCCGAG 
GGCGAACGTA TGCGCCGTCG CACTGGTGTT CCCATGCTAC GGCACACGTA TGGCATCTAC 
AGCCTGGAGC TGAAGACCAA ACTGACGGAA GCCTCCGCCG CCTCGAATAC CCCCGTGGAT 
CTGCTGATGA ATCGAGTCAA GGGGATTTCC CAACGTGTGG ACACAATGCA TCTGAATGAT 
CAGGACACAG CCCTGGATTC GGGTGCCGAC CAGACGGACG AAGAGATCTC CGAGCAGCGG 
CGCCTAGAGC GGAACGAACG AGCGCTGTAC CAGCGCTGCA CCACGGAAGA TCCCAATTGC 
AATGTCAGCG ACAACGACAC GGATACGGCC AGCGCACCGG CCGAAGCCCC ACCGCTGGCC 
GCCACCTACA CAATGTCAAC GCGACGCCGC AAGTCCCCGT CGGCGGCCAA CAAACCCACA 
GTGATTGCCA ACTCCCTGAG GCGCCTGGTC AATCCGGTGG TCACCCATCG TAGTGTGCAC 
AGCGCCACCC CACTGATGAC AACTCCGGGT GTTGTTTATG CCACGCCCGA CGTCACCACC 
TGCACTGAGA AGAAGCTGCG GAAGCCCAGT GGCAATGTAT TCGATGCGGC CCGCCATACC 
ATCGCATCGA CTGTGCGGAT GACGCAGACG GCGCTGGAGA AGAAACAGGC CCAGACGCAG 
GGCGACAAAC TGAATCAGAT CAAGGCGCTG CGACGGCACC ATTCGCGCTC CGTAAATGTT 
AACAGCAACA ACGAGCTGGA CTCGAATCTG AGGCATACGC GAGCCCGTTC TCAGCCCTTC 
CGGAACAACA ACAATGGGGT GACGGCGACA ACCCACGCGA CGATAACCGC GAGATGTGCA 
ACGCAGACCA CGCCCTCCCT GCTGCAAGGA ACAGTGCCGG GAGTGTCGGC GGCAGCGGTG 
GCGGTTGCTT TGGCCAACAA TCTGAACAAT AACACCAGCA ATAGTACCCT CTCTCACAGC 
AGTACTCTAA ACCCATCCCT GAATAACCAG AATTATAATC AGAACAAAAA CAACAATGAT 
GGCACCACTA ACAATCTCCT GCCCGGCAGT CGCACCCGCA GCCTGGCGAT CTACAGCAAG 
AGAATGGAAT TGAAACACCT GCGCAAGGCG GAACAGCAGG AGCAGCCGGT ATCCACTACA 
ACGAACAGCG TCAAGCCGCC ACCGTATGCC ACCATCAACG AGAGCAAGAT CCCTGTGGTG 
ATGATGAGCA GTGCTGCCGC TGGCAGCTCG GCCACCATTC CCCCCACGCG CGGCAGTGCC 
GCCCGCACCT CGCACCACCA CATGACTCTT CGCGGTCGCT CGCGTATCCG CTATCAGCGT 
CCAGCGGCCG GAGAGCCCGC GCCTGCGGCC GCTCAGGCAC AGCCCTGCAC GGTGGCGACG 
GCGCGCTACT ATCGCGATGT GTCCGCCATA CCCACCGCCA TGTCGTCGTT GATTGCTTTG 
GGAACTAGTT CTTCCAACTC TGCTTCCACT TGTACTACTT CTACTGCTGG CTCTGGTTGC 
TATAATATAG CCACACGATC CTCCTCCGCT ACGCTCGATC TGAACTCGAA TCCATTGCCA 
GCGGCCACAC AAGGCACTGG GAGTGGGACT GGGGCTGGGG CCAAGTCCAA GCTATCCAAC 
AACACGAACA ACAACCTCAA CAACAACTGC AATTTGAGTA ATGCCCATTC TTCTGTTTCT 
CCTCCACCTA ACCACAACCA CAACCACCAC CTCCCATCAT CCTCTCCAGA CACAGGCCGT 
CTTAGTGGGC GCGGCAGTGC CGAACTGGAG GCAATCATCG AGCACGTGGG AGCGAGGCGG 
AACAAGCGAT CTCTGAGCTC CACGCGCCTG GACAAGGACA AGTGCGACGA ATACAACACG 
AAACTACTGC GTAAGACGAG CATCGTAGCA GCGGCCGCCG TAGCAGCAGC AGCCTCCGCA 
CCCACTCCTG CCAACCACAA TCCGAACAAC AACAACCATA GTGGAGGCAG CAGCAAATAC 
AGCAGCAACA GTGGCACACC CACAACACCC AGCACTGGAG GCAGCTTCAA GTTGTCGCGT 
CGGGTGTTTG GTGAATCGGG TTCCTCGGCA TCGACATCGA CCTCCAACTC GGGCATACCC 
ACGCCAACGG CACCGACACC GTCATCCACG CAGCAGTGGC AGCAGCACCA TGTGGCACCA 
CGTGTTGGTA GCCGCCTGTC CAGCGATCGG GAGCGAGAGC GCCAACAGCA GGAGCAGCAA 
CAGTCGCTGA AGCTGCGCAA GAAGATCAGC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA20127 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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