Dpse\GA12514 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA12514
Entrez Gene ID	4816114
Full Name	GA12514 gene product from transcript GA12514-RA
Synonyms	GA12514,dpse_GLEANR_7410
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001355731.3	XP_001355767.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA12514 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA12514 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe10420	XM_001355731.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA12514), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA12514 ( XM_001355731.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001355767.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA12514), mRNA.

Price & Availability↑

CloneID	ODe10420
Clone ID Related Accession (Same CDS sequence)	XM_001355731.3
Accession Version	XM_001355731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589962). On Dec 22, 2015 this sequence version replaced XM_001355731.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGTCC TGCATGTCCT GGATGTCGTG GATGTCGCAC AAGCGGCTTC TCTCCACCAG 
CCACAGCCAC AGTCTCAGTC CCAGTCCGAG ACTCCATCCA AGTCCCCAAC AGGAAGCGGG 
CATGGCCAAT TGATTTTGAC GGCAACGAGG ACGAAGACTA GGAGGACTAC GAGGACTAGG 
ACTAGGAGCA AACTAAGACA AACTAAGATA ATTCAATCGC GTTTTTTACA TATTTATGGT 
TACTTTTTCT TCGGTTCCTT GATGAGTTAC ATTAAAACTA TTTGCGCATC AATGGTGCAG 
CAAATGGCAG CGGAAGCGGA TAAAGAAACG GAAGTGGAAG TGGCAGTGGC ATTGCCAGCA 
GCCAAAGCAA AAAGGAAGCC AGTAACGGAA ACGGAAACAG CAACGGGAAT GGGTGTGGGA 
ATGGGAACGG GAACGGATGC CCGCATCGTG GCAACGGGAA TTTGCATTAT AAGACTGATA 
ACATTAAAAA GATTTCTAGA GATTTCCACA ACCGACAAAC ACAATCAAAA GCATCAGCAG 
CAGCAGCATC AGCACCAGCC ATGGCAATCA GCAGCGTCAG CAATGTCACC AACGTCAGTA 
GAAGCGTCAG CTGTTGAAGC AGGAGGAGGA GGAGAAGCAG ATCCAGTCCA AAATAGAAGA 
CACTCACATC CACATACACC CTCGCACTCC CATATACGAC ATCCACAGGC GTGTTGGCGG 
CACCAGCTGA CACCAGGCAT ACTGCTTATG CTCCTTTTGC TCAGCAGCCT CTGGCCGGAC 
TGCTGCGACT GCCTGCACGG CGGCGGCAAT ACGGTGAAGA CCAAATACGG CCTGCTGCGT 
GGGATTGTGG TGCGCTCCAC GCCGCTGGTG GAGGCATTTC TCGGCATACC CTATGCCTCG 
GCGCCTGTGG GCAGTCTCAG ATTTATGCCT CCCATAACGC CATCCACTTG GAAAACGGTT 
CGCAATGCGG ATCGATTCTC GCCCGTCTGT CCACAGAACG TGCCCATTCC ACCCAACGGG 
CCGGAGGCGC TGCTGGAGGT GCCGCGCGCC CGTCTGGCCC AATTGCGGCG TCTGTTGCCG 
CTGCTCAAGA ACCAATCGGA AGACTGCTTA TACCTTAATA TCTATGTGCC CTATGAGACG 
CGCCGCTCGA AACGCAATGT GGACGCTGAT GTTGAGAGTG CAGAAACAAC AACAACAAAA 
CTCTCTACTG TGGTGTTCAT ACATGGCGAA TCGTTCGACT GGAACTCTGG CAATCCGTAC 
GATGGCTCCG AGCTGGCGGC CCACGGCAAT GTCATCGTTG TGACAATCAA TTTCCGCCTG 
GGGATCTTTG GTTTCCTGAA GACCGGCGGC AAGGAGAGCG CCCAAGGTAA CTTTGGTCTG 
ATGGATCTGG TGGCAGGCCT GCACTGGCTC AAGGAGAATC TTCCGGCATT TGGGGGTGAT 
CCGCAAAGCA TTACGCTGCT CGGCTATGGC ACGGGTGCCG TGTTGGCCAA CATATTGGTT 
GTCTCTCCAG TGTCGAGTGA TCTAATACAG CGCACAGTTC TGGTCAGTGG TTCCGCCTTG 
TCGCCCTGGG CCATACAAAA GAATCCACTC TTCGTGAAGC GGCGCGTGGC AGAGCAGACG 
GGTTGCCATG GCGATATGCT GTACGATGAT CTGGCGCCCT GTTTGCGAAC CAAAAGCGTG 
GCCGAACTGC TGGCCGTCAA GGTGGATCAT CCACGATTCC TGGTCGGCTT CGCTCCATTT 
GTGGATGGAA CTGTTATATC GGCCGTTGCC AATCCCTTGG GCAGCACCAC GTTGCCCCTG 
GGGTCAGCTA TTGTTAGTAC TTCAGGAATT GAATATGCAA ACTTTCCGAA ACGAGACCTC 
ATATTCTGCC TTAGTTCTGT GGAATCCTAC CTGGATTTGA GTGCCCAAGA CCTAGAGTTT 
GGCTTTAATG AGACGCGTCG TGATCGCATT CTACGCACTT TTGTGCGCAA TAATTTTCAT 
TATCATTTGA ATGAAATATT TGCCGTCTTG AAGAATGAGT ACACCGACTG GGAGAAAGCC 
ATACGAAATC CTTTGAGCTC CCGCGATGCC ACCCTTCAGT TCCTCAGTGA TGGCCATACG 
GCTTCGCCTC TGATTAAGCT CGGCTACATG CACAGCCTCC GCGGGGGTCG CACTTATTTC 
CTGCACTTTA AGCACAAAAC TGTCGAAGAG GAATACCCAC AGAGAACCGG CTCTGTTCGT 
GGCGAAGATG TGCCTTTTTG GTTAGGTCTG CCCATATCCC CGCTCTTTCC ACACAACTAC 
ACAACTCAGG AGCGTCAAAT CGGTCGCCTA ATGCTGCGAT ATCTGTCCAA CTTTGCCAAG 
ACAGGCAATC CGAACCAATC CACTGAGAAA ACAGCCTTGG GTAATGCAGA AGATGCTACC 
TTGCATCAGC AGAAGAAACG CTCGGCAGCT GTGGTCCCTA ATCCACATCT CAGCGATGCG 
ATGACTTTGG CGGTGCTCTA CAATCAGCGA CGCAGCAATG CCATGCATGA GAAACGCTCC 
TATATACGAC GCCGTTTGCG GAGTAATAAT GATGGTGCCT TCACCCAGCT GGGGCTGGCC 
AGTGAGCGGG ATGTGGGCAG CTACGAGGGT GATGATCTTC CCTTTTGGGA CGCCTACGAT 
GTGGTCAACC AGCTGTATGT GGAGTTGGGC AACAAAGCAA ACATTCAGAG CCATTATCGC 
GGCCATAAGC TGTCCATGTG GTTGAACCTC ATCCCTCAAC TACATCGCCA TTTCAATATC 
AATGACCAGT CCATGAGACA TCATCAATTC CAGGATGATA TGAACAATAG GGATCTGTAT 
GAAGGCGTTG TGCGTCCACA GTTGCAGACG AAACCAGCAG AAGATGATAA CATCATCATT 
ATACAGAAGA GTAGAACGAC CACGACAACG CCGCCACCGC CAAAGACACC TAGTGCAAAT 
GTAACGCAAG CCTTGAATCC CATGATCAGC GGAACAGCTA CCACCACAGA ATGTGGCATC 
GATGGTGCCA TGTCCGTCTC CGAATTGACC ACAACTCAAG CGCCGAAAGA CAATCGAACT 
GGAGTACATA CTCGGGTGGA GACCCAAAAG GACCTGGCCA CCGCTTCGAC GGGCATCATT 
GGCAATCTGG AGCTACTGCG GCGGCTGAGC GGCAAACAGT TCCAAAGCTA CACCACAGCA 
TTGATTGCTA CAGTGGCCGT GGGTTGCTTT TTGCTCATCC TCAATGTCCT CATCTTTGCG 
GGGATCTATC ATCAGCGCGA GAAGCGTGCG CGGGATGCGA AAACCAAGGA GGAGCTGCAG 
GAAAGCGATA CCAGCAAAAG TTCCAGCATG CTCAAGCTGA ATGCTTTAAT TGGGGGTGGT 
GGAGGAGGAC CTGGTGGTTG TGGATCTGTT GATCACGCGG ATGCGTACTC TCTCAGCGGC 
ACTGTGGTGG ACAGCAAGGG CGGTGTGGGT GTTGTCTTTG GGGAATACAG CTGCTACGAT 
GAGAAGACCA AGCAGCTGAA GGAGGAGAAG CTCATTGTGG AGCTGCCACC ATCGTCGTCG 
GTGTCGGTTT CTGGGCAAAC GTCTCTCATG ATGGACAGCT GGGGCGCCTG CTCCACCAGC 
ACACTCGATC TGCTGAAGAC CAAACCAGGC ATCGGAATGG CGGATAGCCA CCTGGAGATG 
GTCTCCTACA ATGCCCTTCC CACGTTAATG GAGTCCACGT CGGCGCTGAG CAGCAAGCGC 
GGATCCTTTG TGGAAAACAG CCTGCAGTTC AACAGTCCGC AGCAGTTCGA CTATGCAGTC 
CAGTCATCAG ATCAGATGTC TTTCAAGGCT ATCGAAGAGG CCGTCAAGGC GGCGGCCATC 
CAAGACTCTG AGATCATGCG AGATGATGAC ATACCCGAAC CGCCGCCCCC ACCGCGATCT 
TTTCTTGCTG CCCAACAGCA ACAACAACAA CAACAACAGC AGCAGCAACA ACAGGGTATA 
CTGCGGCAGA CGGGGACTGC GGGCAGTGCG ACGACGGGTC CAGGGAGCAG CGGGAGCAAG 
AAACGTGTAC ATATTCAGGA AATCTCTGTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001355767.2
CDS	1072..5124
Translation	MDVLHVLDVVDVAQAASLHQPQPQSQSQSETPSKSPTGSGHGQLILTATRTKTRRTTRTRTRSKLRQTKIIQSRFLHIYGYFFFGSLMSYIKTICASMVQQMAAEADKETEVEVAVALPAAKAKRKPVTETETATGMGVGMGTGTDARIVATGICIIRLITLKRFLEISTTDKHNQKHQQQQHQHQPWQSAASAMSPTSVEASAVEAGGGGEADPVQNRRHSHPHTPSHSHIRHPQACWRHQLTPGILLMLLLLSSLWPDCCDCLHGGGNTVKTKYGLLRGIVVRSTPLVEAFLGIPYASAPVGSLRFMPPITPSTWKTVRNADRFSPVCPQNVPIPPNGPEALLEVPRARLAQLRRLLPLLKNQSEDCLYLNIYVPYETRRSKRNVDADVESAETTTTKLSTVVFIHGESFDWNSGNPYDGSELAAHGNVIVVTINFRLGIFGFLKTGGKESAQGNFGLMDLVAGLHWLKENLPAFGGDPQSITLLGYGTGAVLANILVVSPVSSDLIQRTVLVSGSALSPWAIQKNPLFVKRRVAEQTGCHGDMLYDDLAPCLRTKSVAELLAVKVDHPRFLVGFAPFVDGTVISAVANPLGSTTLPLGSAIVSTSGIEYANFPKRDLIFCLSSVESYLDLSAQDLEFGFNETRRDRILRTFVRNNFHYHLNEIFAVLKNEYTDWEKAIRNPLSSRDATLQFLSDGHTASPLIKLGYMHSLRGGRTYFLHFKHKTVEEEYPQRTGSVRGEDVPFWLGLPISPLFPHNYTTQERQIGRLMLRYLSNFAKTGNPNQSTEKTALGNAEDATLHQQKKRSAAVVPNPHLSDAMTLAVLYNQRRSNAMHEKRSYIRRRLRSNNDGAFTQLGLASERDVGSYEGDDLPFWDAYDVVNQLYVELGNKANIQSHYRGHKLSMWLNLIPQLHRHFNINDQSMRHHQFQDDMNNRDLYEGVVRPQLQTKPAEDDNIIIIQKSRTTTTTPPPPKTPSANVTQALNPMISGTATTTECGIDGAMSVSELTTTQAPKDNRTGVHTRVETQKDLATASTGIIGNLELLRRLSGKQFQSYTTALIATVAVGCFLLILNVLIFAGIYHQREKRARDAKTKEELQESDTSKSSSMLKLNALIGGGGGGPGGCGSVDHADAYSLSGTVVDSKGGVGVVFGEYSCYDEKTKQLKEEKLIVELPPSSSVSVSGQTSLMMDSWGACSTSTLDLLKTKPGIGMADSHLEMVSYNALPTLMESTSALSSKRGSFVENSLQFNSPQQFDYAVQSSDQMSFKAIEEAVKAAAIQDSEIMRDDDIPEPPPPPRSFLAAQQQQQQQQQQQQQQGILRQTGTAGSATTGPGSSGSKKRVHIQEISV

Target ORF information:

RefSeq Version	XM_001355731.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA12514), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001355731.3

ORF Insert Sequence:

ATGGATGTCC TGCATGTCCT GGATGTCGTG GATGTCGCAC AAGCGGCTTC TCTCCACCAG 
CCACAGCCAC AGTCTCAGTC CCAGTCCGAG ACTCCATCCA AGTCCCCAAC AGGAAGCGGG 
CATGGCCAAT TGATTTTGAC GGCAACGAGG ACGAAGACTA GGAGGACTAC GAGGACTAGG 
ACTAGGAGCA AACTAAGACA AACTAAGATA ATTCAATCGC GTTTTTTACA TATTTATGGT 
TACTTTTTCT TCGGTTCCTT GATGAGTTAC ATTAAAACTA TTTGCGCATC AATGGTGCAG 
CAAATGGCAG CGGAAGCGGA TAAAGAAACG GAAGTGGAAG TGGCAGTGGC ATTGCCAGCA 
GCCAAAGCAA AAAGGAAGCC AGTAACGGAA ACGGAAACAG CAACGGGAAT GGGTGTGGGA 
ATGGGAACGG GAACGGATGC CCGCATCGTG GCAACGGGAA TTTGCATTAT AAGACTGATA 
ACATTAAAAA GATTTCTAGA GATTTCCACA ACCGACAAAC ACAATCAAAA GCATCAGCAG 
CAGCAGCATC AGCACCAGCC ATGGCAATCA GCAGCGTCAG CAATGTCACC AACGTCAGTA 
GAAGCGTCAG CTGTTGAAGC AGGAGGAGGA GGAGAAGCAG ATCCAGTCCA AAATAGAAGA 
CACTCACATC CACATACACC CTCGCACTCC CATATACGAC ATCCACAGGC GTGTTGGCGG 
CACCAGCTGA CACCAGGCAT ACTGCTTATG CTCCTTTTGC TCAGCAGCCT CTGGCCGGAC 
TGCTGCGACT GCCTGCACGG CGGCGGCAAT ACGGTGAAGA CCAAATACGG CCTGCTGCGT 
GGGATTGTGG TGCGCTCCAC GCCGCTGGTG GAGGCATTTC TCGGCATACC CTATGCCTCG 
GCGCCTGTGG GCAGTCTCAG ATTTATGCCT CCCATAACGC CATCCACTTG GAAAACGGTT 
CGCAATGCGG ATCGATTCTC GCCCGTCTGT CCACAGAACG TGCCCATTCC ACCCAACGGG 
CCGGAGGCGC TGCTGGAGGT GCCGCGCGCC CGTCTGGCCC AATTGCGGCG TCTGTTGCCG 
CTGCTCAAGA ACCAATCGGA AGACTGCTTA TACCTTAATA TCTATGTGCC CTATGAGACG 
CGCCGCTCGA AACGCAATGT GGACGCTGAT GTTGAGAGTG CAGAAACAAC AACAACAAAA 
CTCTCTACTG TGGTGTTCAT ACATGGCGAA TCGTTCGACT GGAACTCTGG CAATCCGTAC 
GATGGCTCCG AGCTGGCGGC CCACGGCAAT GTCATCGTTG TGACAATCAA TTTCCGCCTG 
GGGATCTTTG GTTTCCTGAA GACCGGCGGC AAGGAGAGCG CCCAAGGTAA CTTTGGTCTG 
ATGGATCTGG TGGCAGGCCT GCACTGGCTC AAGGAGAATC TTCCGGCATT TGGGGGTGAT 
CCGCAAAGCA TTACGCTGCT CGGCTATGGC ACGGGTGCCG TGTTGGCCAA CATATTGGTT 
GTCTCTCCAG TGTCGAGTGA TCTAATACAG CGCACAGTTC TGGTCAGTGG TTCCGCCTTG 
TCGCCCTGGG CCATACAAAA GAATCCACTC TTCGTGAAGC GGCGCGTGGC AGAGCAGACG 
GGTTGCCATG GCGATATGCT GTACGATGAT CTGGCGCCCT GTTTGCGAAC CAAAAGCGTG 
GCCGAACTGC TGGCCGTCAA GGTGGATCAT CCACGATTCC TGGTCGGCTT CGCTCCATTT 
GTGGATGGAA CTGTTATATC GGCCGTTGCC AATCCCTTGG GCAGCACCAC GTTGCCCCTG 
GGGTCAGCTA TTGTTAGTAC TTCAGGAATT GAATATGCAA ACTTTCCGAA ACGAGACCTC 
ATATTCTGCC TTAGTTCTGT GGAATCCTAC CTGGATTTGA GTGCCCAAGA CCTAGAGTTT 
GGCTTTAATG AGACGCGTCG TGATCGCATT CTACGCACTT TTGTGCGCAA TAATTTTCAT 
TATCATTTGA ATGAAATATT TGCCGTCTTG AAGAATGAGT ACACCGACTG GGAGAAAGCC 
ATACGAAATC CTTTGAGCTC CCGCGATGCC ACCCTTCAGT TCCTCAGTGA TGGCCATACG 
GCTTCGCCTC TGATTAAGCT CGGCTACATG CACAGCCTCC GCGGGGGTCG CACTTATTTC 
CTGCACTTTA AGCACAAAAC TGTCGAAGAG GAATACCCAC AGAGAACCGG CTCTGTTCGT 
GGCGAAGATG TGCCTTTTTG GTTAGGTCTG CCCATATCCC CGCTCTTTCC ACACAACTAC 
ACAACTCAGG AGCGTCAAAT CGGTCGCCTA ATGCTGCGAT ATCTGTCCAA CTTTGCCAAG 
ACAGGCAATC CGAACCAATC CACTGAGAAA ACAGCCTTGG GTAATGCAGA AGATGCTACC 
TTGCATCAGC AGAAGAAACG CTCGGCAGCT GTGGTCCCTA ATCCACATCT CAGCGATGCG 
ATGACTTTGG CGGTGCTCTA CAATCAGCGA CGCAGCAATG CCATGCATGA GAAACGCTCC 
TATATACGAC GCCGTTTGCG GAGTAATAAT GATGGTGCCT TCACCCAGCT GGGGCTGGCC 
AGTGAGCGGG ATGTGGGCAG CTACGAGGGT GATGATCTTC CCTTTTGGGA CGCCTACGAT 
GTGGTCAACC AGCTGTATGT GGAGTTGGGC AACAAAGCAA ACATTCAGAG CCATTATCGC 
GGCCATAAGC TGTCCATGTG GTTGAACCTC ATCCCTCAAC TACATCGCCA TTTCAATATC 
AATGACCAGT CCATGAGACA TCATCAATTC CAGGATGATA TGAACAATAG GGATCTGTAT 
GAAGGCGTTG TGCGTCCACA GTTGCAGACG AAACCAGCAG AAGATGATAA CATCATCATT 
ATACAGAAGA GTAGAACGAC CACGACAACG CCGCCACCGC CAAAGACACC TAGTGCAAAT 
GTAACGCAAG CCTTGAATCC CATGATCAGC GGAACAGCTA CCACCACAGA ATGTGGCATC 
GATGGTGCCA TGTCCGTCTC CGAATTGACC ACAACTCAAG CGCCGAAAGA CAATCGAACT 
GGAGTACATA CTCGGGTGGA GACCCAAAAG GACCTGGCCA CCGCTTCGAC GGGCATCATT 
GGCAATCTGG AGCTACTGCG GCGGCTGAGC GGCAAACAGT TCCAAAGCTA CACCACAGCA 
TTGATTGCTA CAGTGGCCGT GGGTTGCTTT TTGCTCATCC TCAATGTCCT CATCTTTGCG 
GGGATCTATC ATCAGCGCGA GAAGCGTGCG CGGGATGCGA AAACCAAGGA GGAGCTGCAG 
GAAAGCGATA CCAGCAAAAG TTCCAGCATG CTCAAGCTGA ATGCTTTAAT TGGGGGTGGT 
GGAGGAGGAC CTGGTGGTTG TGGATCTGTT GATCACGCGG ATGCGTACTC TCTCAGCGGC 
ACTGTGGTGG ACAGCAAGGG CGGTGTGGGT GTTGTCTTTG GGGAATACAG CTGCTACGAT 
GAGAAGACCA AGCAGCTGAA GGAGGAGAAG CTCATTGTGG AGCTGCCACC ATCGTCGTCG 
GTGTCGGTTT CTGGGCAAAC GTCTCTCATG ATGGACAGCT GGGGCGCCTG CTCCACCAGC 
ACACTCGATC TGCTGAAGAC CAAACCAGGC ATCGGAATGG CGGATAGCCA CCTGGAGATG 
GTCTCCTACA ATGCCCTTCC CACGTTAATG GAGTCCACGT CGGCGCTGAG CAGCAAGCGC 
GGATCCTTTG TGGAAAACAG CCTGCAGTTC AACAGTCCGC AGCAGTTCGA CTATGCAGTC 
CAGTCATCAG ATCAGATGTC TTTCAAGGCT ATCGAAGAGG CCGTCAAGGC GGCGGCCATC 
CAAGACTCTG AGATCATGCG AGATGATGAC ATACCCGAAC CGCCGCCCCC ACCGCGATCT 
TTTCTTGCTG CCCAACAGCA ACAACAACAA CAACAACAGC AGCAGCAACA ACAGGGTATA 
CTGCGGCAGA CGGGGACTGC GGGCAGTGCG ACGACGGGTC CAGGGAGCAG CGGGAGCAAG 
AAACGTGTAC ATATTCAGGA AATCTCTGTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA12514 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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