Dpse\GA20743 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA20743
Entrez Gene ID	4801730
Full Name	GA20743 gene product from transcript GA20743-RA
Synonyms	GA20743,dpse_GLEANR_3836
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001358736.3	XP_001358773.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA20743 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA20743 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe01317	XM_001358736.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20743), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA20743 ( XM_001358736.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001358773.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20743), mRNA.

Price & Availability↑

CloneID	ODe01317
Clone ID Related Accession (Same CDS sequence)	XM_001358736.3
Accession Version	XM_001358736.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001358736.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCACC AGAACTATGG CGGCATCCAA TGGATACAGC AACACCCCGC CGCATCCGAC 
TCCGGGGTGG ACATACTGGT GGGGGACGGC GAAGACGGTG TGACGCCGGA CGGGTATCAT 
CTCGTGACCG ATGAAGCGAC GGGCGAGGAT CTTGACCATA TGGCGGCCGA GAATCTCATA 
TTTATGGCCC GCGACGGCCA GCTGAGTGAG ATCATAGCTG GCGGAAACGC TGTGATAGTC 
ACTGCCGATG GCCACGGCGT GACGTACGCC GATTCCTTTG ACGATGACGC CCAGGTGGTC 
ACCGAGGAGG TCATCACGGA CGACTGGGTG CAGCATCAAG GAGCCGAGCG TGTGGAAATA 
GCCGCGGAGC AAATAGCTGG AAATGGAAGC TCGCAAGAGC TGCTGGACAT GGAGCAGGAC 
GAGTACACGG CCCTGCGACC ATATCCCTGC GACTTTTGCA GCCGCCGCTT TAAGAAGAAG 
TCCAGCCTGA ATACCCACAT GCTGGCCCAC CAAAACGATC GTCCTCATCT TTGCAAGCTG 
TGCGGGTCAC GCTTCTCTCG CCGCGCCGAG CTCATCAGTC ATTTCAAGGC GCACGCGGAA 
GCTCAGGATG CAGCGGATGC CGAGGCAGCT GCGGCCACAT CCATTAAATT CGAGCACCGC 
CCAATGGACG ATCATTACTA TGAGCACGAG TGGCCTTTAT ACCAGCAGGA TCAGGACGCC 
GAGGACCAGC AGCAGATGGT GTCCAGCAGC GAAACTCAAC TGGTGCACCA GGATAGCTAC 
AACGTTGCAG CAGTTACCGT CGATGCTCCA GTGCCAACGC GAGGACGGAT CAGTCGCCTG 
AAGCCAAAGG AGGAAGCTTC ACAGTACATT GTGATTTCCG ACAAGACGGA AGCACCCCAG 
ATGCAGGACT ATATGCAAAG CGAGCCGATG CAGGCGGTGG TCACCACCTC GACAGCGATC 
GAGCCCCCAC CTAAAGCTGA CCTTCCCCAG CCGAACTATC CAGTGCTGGA CGACAGCAAG 
CCGTTCGTGT GTCAGCAGTG CGGCTTGGCC TTTGCCAGGG AGAAGGCGCT CTTCTCTCAC 
ACGAAGAACC ATCGCGTGGA CTCGCCCTTT GAGTGCAACC TGTGTCAGGA GATGTTCTGG 
GATAACAGCA GCCTTCTGGA GCATCAGAAG ACGCATCAGT TCGAGGAGAG CAACTCTGAG 
TACGATCCGG CATCGGGGGA CGACAGTGTC AGCGAGTCGG AACCGGATCA GCTCTATGGC 
GACTTTTACT GCAGCGAATG CGGCATTTGC TTTCACAGGC AGGACCTGCT GCGACGCCAT 
GCAAAGTGCC ATTTCCAGCA GCCAGACGCA AGTAACTTTG ACCAAAAGCC GATTGTGGGA 
GAAGTGGGAG TCACCGATGA AGTGATAGCT GGAGATGCTG AAAATGCCAA AGATGATGCT 
GCCCACCGGT GTCACACGTG CGGCAAGTGT TTTCCCAATT CAGTTGCGTT GAGCGCCCAT 
GCCGAAATGC ACGCCCGTTT TCCGCTCTTC AAGTGCGTTT TGTGCGGTGT CAGCTTCTAT 
GAAGAGCAGT CCATCAAGCG GCACTTGCAC AGTCGTCATC CGAACGAGCT GAAGGCCAAC 
TCGTGTGTGC TGTGTGGAAA GGAGTGTCGC GATCGGAAGG CTCTCATCAA GCATGCCTGG 
GACCACTCGA GGGAGAAGTG CCACTCGTGC TCCAAGTGCG GCAAGAACTT CCATAACAAG 
GCGCGTCTGA AGCGGCATAT GGCCTCGCAT CGGGACAAGT CGGTGGTGTG TGAGGTCTGT 
CAGGAGGAAT TCCCCGACGG ACGGACGCTA TCCAATCACC GTCACTCCCA CAGCACCACC 
TCGCCGGGCA AACTCTTCCC GTGCCTGGAG TGTGGCAAAA CCTTCGGTTC GCGCAGCTCT 
CAGCAGATTC ACGTACGCAT CCACACGGGC GAGCGTCCGT ACGGCTGTCG GTACTGCTGG 
AAGGCCTTTG CCGACGGCGG CACTTTGCGC AAACACGAGC GCATCCACAC GGGCGAGAAG 
CCCTACGCCT GCTCCGTATG TCCACGTGCC TTCAACCAGC GAGTGGTGCT CCGCGAGCAC 
ATTCGCTCCC ATCACTCCGG TATGGATGTA ACGCTTGGCA CTTACCACTG CGAAGTGTGC 
TCCGAGGACT TGTCCTCGTC CAGTGAGCTC ATCCAGCATC TTATCAAGCA CAGCGACACG 
AACACCGCCA AACAGCGTCA GCCCGTCACG GGGCCTCGGA AGTACAAACG ACGGCGCAAG 
CTGCAGCCGC ATGAGATTGC CCACATGCGG GCCGAGAACA AGGAAGATGA GCTTTTGCAA 
GAGGCCGAAG AGTATAACAG TGACTTAGAC ATCTGCGACT TTGATGTGGA GAACGTGGAC 
CGAATCTTCG AAATGGCCGA ATCCCCCAAA AAGGAGAAGC GCAAAAAGGG AGCAGCCAAG 
GATCCGAAGG CGGTGGCCAA TGGAACAACC AAGGCTGTCA CAGATAAGTC GAAAATGCGC 
TACGATTCCA CCAGCAGTCA CTCGGATCGC ATGTGGGAGG AGAAGTTCCT GCAAGACAAC 
CAGGTGTCGT TGTTTCAAAT TGATTCGTTA GCGGCTATGA ATCAACAGCA GTCACCGCTG 
CCTACCAGCA CCGCCAGTTC CTCCAGCGTC CAAAACACGA GACGCAGTTC CAAGCAGTCA 
CAGGCCCAAG GGCAGGCCAA GACGGCGCCA CCAGTTGAGG TAAAAGCAAA CACCAGTGCA 
TCATCCTCCT CCACGACTGC GAGCTCCAGC AGCCGCAGCC GAAAGAAAGC GGGAACCAGT 
AAGGCGGCCA GTAGCAAATC GGCTGCTGCG GGAAGCTCGC GTCCGCGAAT GATCCACACC 
GAGAAGGCCA AGGTTCCGAA GTCTTCAGAA AGCTCGGGCA CTGCATCCGC ATCTACCAAG 
AAGACGCGTG GCAAGAGTTA CATTACCCGG ACAGAGACAA GCAATCTGGA TTTGCTTTCC 
AGCAGCGGCA GCGGCAAGTC GCGATCCACT GCGTCCAACA TTGTCGACGA CTATGAGATC 
ATTTCCCCAG CCACGCATCC ACCCAACCAG ATCAGCTCCA GTCCCCTCCA CATTAAGCAG 
GAGCAAGAAC AGCAGCGCGT TCCGATGATG TACGACGACA ATCTGCTCAT CGATGCCGCA 
CTGCTCCGTG AAAAGACGTA CGAGAGGTTC AACCCCGCAA TAGTCAACGA TTTGGAGGAG 
ATTCTGCGCT CTCCACTCAA AAGTGAGCAC AATTCCCGAC TGCGTTTCAC CAGCGAGTCA 
TCGACAACGC CACAGTTTCT GCCCGGGGAG GAGCAGAACC TCATCAAGAT CGAGCCGACA 
TCCCCCGACT TACGGGAAAT GGTGGAGAGT CAACAGCGCG TCACGGCTAC CACTGGCAAC 
ACGCCCTCTT CCTCGTCGTC GTCGGCCCGT ACCTCGCGTC ACCTGCGCCA GATAACTGCC 
AACAGCCCGC GAGTGGGCAG CAAACGTTCC AGCAGCAGCA GGCCGGACTC TGCATCCGGC 
ACGGCAGCCA AGAAGCCCAC CACATCGTCC TCCTCTTCGC CAGTCGCAGC CGGGAGCAAG 
ACCAGAAGCT CCGAACACTC GTACCTCAGC TACGGCGTGG GCGTGGACTC CTACAATGTG 
AATGTACCTG CCAGCGCCAA CACTGCCGAT GTAAGCTCTG CATTCTTTTC CATTTCCGAG 
GTGGTTTCCG CCGCCGACGC AGCCGATGCT GCAGCCAAGG CAGCCGCAGC CAGCAAGTCG 
GACCGCAAGA AGCGCAGCTT CGAATGCGAG ATGTGCTCCG CCATCTTCTA CGACCGCGCG 
CAGCTGCTGG ACCACGTCCA CATCCACATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001358773.1
CDS	114..3986
Translation	MNHQNYGGIQWIQQHPAASDSGVDILVGDGEDGVTPDGYHLVTDEATGEDLDHMAAENLIFMARDGQLSEIIAGGNAVIVTADGHGVTYADSFDDDAQVVTEEVITDDWVQHQGAERVEIAAEQIAGNGSSQELLDMEQDEYTALRPYPCDFCSRRFKKKSSLNTHMLAHQNDRPHLCKLCGSRFSRRAELISHFKAHAEAQDAADAEAAAATSIKFEHRPMDDHYYEHEWPLYQQDQDAEDQQQMVSSSETQLVHQDSYNVAAVTVDAPVPTRGRISRLKPKEEASQYIVISDKTEAPQMQDYMQSEPMQAVVTTSTAIEPPPKADLPQPNYPVLDDSKPFVCQQCGLAFAREKALFSHTKNHRVDSPFECNLCQEMFWDNSSLLEHQKTHQFEESNSEYDPASGDDSVSESEPDQLYGDFYCSECGICFHRQDLLRRHAKCHFQQPDASNFDQKPIVGEVGVTDEVIAGDAENAKDDAAHRCHTCGKCFPNSVALSAHAEMHARFPLFKCVLCGVSFYEEQSIKRHLHSRHPNELKANSCVLCGKECRDRKALIKHAWDHSREKCHSCSKCGKNFHNKARLKRHMASHRDKSVVCEVCQEEFPDGRTLSNHRHSHSTTSPGKLFPCLECGKTFGSRSSQQIHVRIHTGERPYGCRYCWKAFADGGTLRKHERIHTGEKPYACSVCPRAFNQRVVLREHIRSHHSGMDVTLGTYHCEVCSEDLSSSSELIQHLIKHSDTNTAKQRQPVTGPRKYKRRRKLQPHEIAHMRAENKEDELLQEAEEYNSDLDICDFDVENVDRIFEMAESPKKEKRKKGAAKDPKAVANGTTKAVTDKSKMRYDSTSSHSDRMWEEKFLQDNQVSLFQIDSLAAMNQQQSPLPTSTASSSSVQNTRRSSKQSQAQGQAKTAPPVEVKANTSASSSSTTASSSSRSRKKAGTSKAASSKSAAAGSSRPRMIHTEKAKVPKSSESSGTASASTKKTRGKSYITRTETSNLDLLSSSGSGKSRSTASNIVDDYEIISPATHPPNQISSSPLHIKQEQEQQRVPMMYDDNLLIDAALLREKTYERFNPAIVNDLEEILRSPLKSEHNSRLRFTSESSTTPQFLPGEEQNLIKIEPTSPDLREMVESQQRVTATTGNTPSSSSSSARTSRHLRQITANSPRVGSKRSSSSRPDSASGTAAKKPTTSSSSSPVAAGSKTRSSEHSYLSYGVGVDSYNVNVPASANTADVSSAFFSISEVVSAADAADAAAKAAAASKSDRKKRSFECEMCSAIFYDRAQLLDHVHIHI

Target ORF information:

RefSeq Version	XM_001358736.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20743), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001358736.3

ORF Insert Sequence:

ATGAACCACC AGAACTATGG CGGCATCCAA TGGATACAGC AACACCCCGC CGCATCCGAC 
TCCGGGGTGG ACATACTGGT GGGGGACGGC GAAGACGGTG TGACGCCGGA CGGGTATCAT 
CTCGTGACCG ATGAAGCGAC GGGCGAGGAT CTTGACCATA TGGCGGCCGA GAATCTCATA 
TTTATGGCCC GCGACGGCCA GCTGAGTGAG ATCATAGCTG GCGGAAACGC TGTGATAGTC 
ACTGCCGATG GCCACGGCGT GACGTACGCC GATTCCTTTG ACGATGACGC CCAGGTGGTC 
ACCGAGGAGG TCATCACGGA CGACTGGGTG CAGCATCAAG GAGCCGAGCG TGTGGAAATA 
GCCGCGGAGC AAATAGCTGG AAATGGAAGC TCGCAAGAGC TGCTGGACAT GGAGCAGGAC 
GAGTACACGG CCCTGCGACC ATATCCCTGC GACTTTTGCA GCCGCCGCTT TAAGAAGAAG 
TCCAGCCTGA ATACCCACAT GCTGGCCCAC CAAAACGATC GTCCTCATCT TTGCAAGCTG 
TGCGGGTCAC GCTTCTCTCG CCGCGCCGAG CTCATCAGTC ATTTCAAGGC GCACGCGGAA 
GCTCAGGATG CAGCGGATGC CGAGGCAGCT GCGGCCACAT CCATTAAATT CGAGCACCGC 
CCAATGGACG ATCATTACTA TGAGCACGAG TGGCCTTTAT ACCAGCAGGA TCAGGACGCC 
GAGGACCAGC AGCAGATGGT GTCCAGCAGC GAAACTCAAC TGGTGCACCA GGATAGCTAC 
AACGTTGCAG CAGTTACCGT CGATGCTCCA GTGCCAACGC GAGGACGGAT CAGTCGCCTG 
AAGCCAAAGG AGGAAGCTTC ACAGTACATT GTGATTTCCG ACAAGACGGA AGCACCCCAG 
ATGCAGGACT ATATGCAAAG CGAGCCGATG CAGGCGGTGG TCACCACCTC GACAGCGATC 
GAGCCCCCAC CTAAAGCTGA CCTTCCCCAG CCGAACTATC CAGTGCTGGA CGACAGCAAG 
CCGTTCGTGT GTCAGCAGTG CGGCTTGGCC TTTGCCAGGG AGAAGGCGCT CTTCTCTCAC 
ACGAAGAACC ATCGCGTGGA CTCGCCCTTT GAGTGCAACC TGTGTCAGGA GATGTTCTGG 
GATAACAGCA GCCTTCTGGA GCATCAGAAG ACGCATCAGT TCGAGGAGAG CAACTCTGAG 
TACGATCCGG CATCGGGGGA CGACAGTGTC AGCGAGTCGG AACCGGATCA GCTCTATGGC 
GACTTTTACT GCAGCGAATG CGGCATTTGC TTTCACAGGC AGGACCTGCT GCGACGCCAT 
GCAAAGTGCC ATTTCCAGCA GCCAGACGCA AGTAACTTTG ACCAAAAGCC GATTGTGGGA 
GAAGTGGGAG TCACCGATGA AGTGATAGCT GGAGATGCTG AAAATGCCAA AGATGATGCT 
GCCCACCGGT GTCACACGTG CGGCAAGTGT TTTCCCAATT CAGTTGCGTT GAGCGCCCAT 
GCCGAAATGC ACGCCCGTTT TCCGCTCTTC AAGTGCGTTT TGTGCGGTGT CAGCTTCTAT 
GAAGAGCAGT CCATCAAGCG GCACTTGCAC AGTCGTCATC CGAACGAGCT GAAGGCCAAC 
TCGTGTGTGC TGTGTGGAAA GGAGTGTCGC GATCGGAAGG CTCTCATCAA GCATGCCTGG 
GACCACTCGA GGGAGAAGTG CCACTCGTGC TCCAAGTGCG GCAAGAACTT CCATAACAAG 
GCGCGTCTGA AGCGGCATAT GGCCTCGCAT CGGGACAAGT CGGTGGTGTG TGAGGTCTGT 
CAGGAGGAAT TCCCCGACGG ACGGACGCTA TCCAATCACC GTCACTCCCA CAGCACCACC 
TCGCCGGGCA AACTCTTCCC GTGCCTGGAG TGTGGCAAAA CCTTCGGTTC GCGCAGCTCT 
CAGCAGATTC ACGTACGCAT CCACACGGGC GAGCGTCCGT ACGGCTGTCG GTACTGCTGG 
AAGGCCTTTG CCGACGGCGG CACTTTGCGC AAACACGAGC GCATCCACAC GGGCGAGAAG 
CCCTACGCCT GCTCCGTATG TCCACGTGCC TTCAACCAGC GAGTGGTGCT CCGCGAGCAC 
ATTCGCTCCC ATCACTCCGG TATGGATGTA ACGCTTGGCA CTTACCACTG CGAAGTGTGC 
TCCGAGGACT TGTCCTCGTC CAGTGAGCTC ATCCAGCATC TTATCAAGCA CAGCGACACG 
AACACCGCCA AACAGCGTCA GCCCGTCACG GGGCCTCGGA AGTACAAACG ACGGCGCAAG 
CTGCAGCCGC ATGAGATTGC CCACATGCGG GCCGAGAACA AGGAAGATGA GCTTTTGCAA 
GAGGCCGAAG AGTATAACAG TGACTTAGAC ATCTGCGACT TTGATGTGGA GAACGTGGAC 
CGAATCTTCG AAATGGCCGA ATCCCCCAAA AAGGAGAAGC GCAAAAAGGG AGCAGCCAAG 
GATCCGAAGG CGGTGGCCAA TGGAACAACC AAGGCTGTCA CAGATAAGTC GAAAATGCGC 
TACGATTCCA CCAGCAGTCA CTCGGATCGC ATGTGGGAGG AGAAGTTCCT GCAAGACAAC 
CAGGTGTCGT TGTTTCAAAT TGATTCGTTA GCGGCTATGA ATCAACAGCA GTCACCGCTG 
CCTACCAGCA CCGCCAGTTC CTCCAGCGTC CAAAACACGA GACGCAGTTC CAAGCAGTCA 
CAGGCCCAAG GGCAGGCCAA GACGGCGCCA CCAGTTGAGG TAAAAGCAAA CACCAGTGCA 
TCATCCTCCT CCACGACTGC GAGCTCCAGC AGCCGCAGCC GAAAGAAAGC GGGAACCAGT 
AAGGCGGCCA GTAGCAAATC GGCTGCTGCG GGAAGCTCGC GTCCGCGAAT GATCCACACC 
GAGAAGGCCA AGGTTCCGAA GTCTTCAGAA AGCTCGGGCA CTGCATCCGC ATCTACCAAG 
AAGACGCGTG GCAAGAGTTA CATTACCCGG ACAGAGACAA GCAATCTGGA TTTGCTTTCC 
AGCAGCGGCA GCGGCAAGTC GCGATCCACT GCGTCCAACA TTGTCGACGA CTATGAGATC 
ATTTCCCCAG CCACGCATCC ACCCAACCAG ATCAGCTCCA GTCCCCTCCA CATTAAGCAG 
GAGCAAGAAC AGCAGCGCGT TCCGATGATG TACGACGACA ATCTGCTCAT CGATGCCGCA 
CTGCTCCGTG AAAAGACGTA CGAGAGGTTC AACCCCGCAA TAGTCAACGA TTTGGAGGAG 
ATTCTGCGCT CTCCACTCAA AAGTGAGCAC AATTCCCGAC TGCGTTTCAC CAGCGAGTCA 
TCGACAACGC CACAGTTTCT GCCCGGGGAG GAGCAGAACC TCATCAAGAT CGAGCCGACA 
TCCCCCGACT TACGGGAAAT GGTGGAGAGT CAACAGCGCG TCACGGCTAC CACTGGCAAC 
ACGCCCTCTT CCTCGTCGTC GTCGGCCCGT ACCTCGCGTC ACCTGCGCCA GATAACTGCC 
AACAGCCCGC GAGTGGGCAG CAAACGTTCC AGCAGCAGCA GGCCGGACTC TGCATCCGGC 
ACGGCAGCCA AGAAGCCCAC CACATCGTCC TCCTCTTCGC CAGTCGCAGC CGGGAGCAAG 
ACCAGAAGCT CCGAACACTC GTACCTCAGC TACGGCGTGG GCGTGGACTC CTACAATGTG 
AATGTACCTG CCAGCGCCAA CACTGCCGAT GTAAGCTCTG CATTCTTTTC CATTTCCGAG 
GTGGTTTCCG CCGCCGACGC AGCCGATGCT GCAGCCAAGG CAGCCGCAGC CAGCAAGTCG 
GACCGCAAGA AGCGCAGCTT CGAATGCGAG ATGTGCTCCG CCATCTTCTA CGACCGCGCG 
CAGCTGCTGG ACCACGTCCA CATCCACATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA20743 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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