Dpse\GA10930 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA10930
Entrez Gene ID	4800726
Full Name	GA10930 gene product from transcript GA10930-RA
Synonyms	GA10930,dpse_GLEANR_4435
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001357909.4	XP_001357946.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA10930 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA10930 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe00414	XM_001357909.4 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA10930), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA10930 ( XM_001357909.4 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001357946.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA10930), mRNA.

Price & Availability↑

CloneID	ODe00414
Clone ID Related Accession (Same CDS sequence)	XM_001357909.4
Accession Version	XM_001357909.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001357909.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTTTCA CGAGGAAAAC ACTGAAATTG CTAAATTACC GCCTGAAAAG CATTGCCGTC 
GCCCGCCTAA GTGCCCATCG ATGCCTCCAA AGCAGCGCCA GCGGAGAAGT GCCCTCCGTG 
GAGGTGAAGT TCAGCAATGG ACGTAACATG AGCATCAGCT CGGGGAAGCT GGCGCGCTTT 
GCCAATGGCA CTGCCGTCTG CCAGATGGGA GACACCGCCG TGATGGTGAC AGCGGTGGCC 
AAGCCGAAGC CCACTCCCGG CCAGAACTTT ATGCCGCTGG TGGTGGACTA TCGCCTGAAG 
AACGCCGCCT CGGGGCGAAT TCCAATGAAC TTTATGCGCC GCGAGCTGGG TCCCTCCGAG 
AAGGAGATCC TGTCGGCCCG CCTGATCGAT CGCTCGCTGC GTCCGCTGTT CAACAAAGAC 
TACCGCACGG AGACGCAGCT GGTGTGCAAC ATGCTGGCCA TGGACGCGGT GCACTCGCCC 
GACATTCTAG CCATCAATGC CGCCTCCATG GCCCTGTCCC TGAGCGATAT ACCCTGGAAT 
GGTCCGATTG GAGCTGTGCG GGTTGGCCTT TCCGACGGCG AGGTGCTGAT CAATCCCACC 
CGCCGGGAGC TGCAGTCCTC GCAGCTGGAC CTCGTGGTGT CGGCCACCAA ACAGAACCTC 
GTCGTGATGC TCGAAGGCAA GGGCAATGTT GTGTTGATGC AGGACCTCCT GAAGGCCATA 
AAGCAGGGCA CGCGGGAGGC ACAGTTCATC ATCCAAGAGA TCGAGCGCCT GCAGAAGGCC 
TACGGCAGGA AGAAACGCGA GGTGGAGTCC CCGAACCGAG TGGATCCCGA GCTGCAGGAG 
GCGGTGCGCA GCATGTGCGA GATGCGGCTG CGAGAGATCT TCCAGGATGC GAATCACGAC 
AAGATCTCGC GCGACAACGC AGTCAACGAG GTGCGCTCGA ATGTGATCGA CAAGGTGTGG 
TCCTCCTATC CGGACACCGA GCCAGGGGTG ATCGGCGAGG AGTTCAATCA GACGTGCCGC 
TCGATCTTCC GGGAGCTAAT CTTCGAGCGG AATTACCGCT GCGATGGCAG GGACTATGAC 
ACGCTGCGGA ACATCTCCTG CCAGGTGGAC ATGTACAAGC CGCTGCACGG CTCGGCCTTG 
TTCCAGCGCG GACAGACGCA GGTCTTCTGC ACCGTGAGCC TGGACTCCCC GGAGAGTGCC 
ATGAAGCTGG ACTCGCTGGC CGCCCTCGAC AGTGGCGGCC TCAAGGCCAA GAACTTCATG 
CTCCACTACG AGTTCCCGCC GTACGCCACG GGGGAGGTGG GCAGGATTGG CCCGCTGGGT 
CGGAGGGAGC TGGGACACGG CGCCCTGGCG GAGAGATCTC TGTTGCCCAC ATTGCCACAC 
GACTACCCCT TCACGGTTCG CCTCACCTCC GAGGTGCTCG AGTCGAACGG TTCCAGCAGC 
ATGGCCAGCG TCTGCGGCGG ATCCCTGGCC CTGATGGATG CCGGGGTGCC GGTGAGTGCC 
CCTGCCGCGG GGGTGGCCAT TGGTTTGGTC ACCAAGTACG AGAACGACGA CACCAAGCAC 
CTGCAGGACT ATCGCATTCT CACCGACATC CTGGGCATCG AGGACTACAT GGGGGACATG 
GACATGAAGG TGGCGGGCAC ACGCAAGGGA TTCACAGCCA TCCAGGCGGA CCTCAAGGTG 
CCTGGCATTC CATTGAAAGT GGTGATGGAA TCCCTGCAAA AAGCCACCGA TGCCAAGTCC 
AAAATACTGG ACATCATGGC CGAGGCCATT CGAGAGCCAC GGAAATACCC AAAGGACAGC 
TGGCCCGTGA GCGAAACCAT CAGTGTGGAG CCGCACCAGC GCTCCCAGTT GATTGGCCCC 
AGTGGCCTGC ACATGAAACG CATCTACCTG GAGACGGGCG CCACCCTCAC AGCCGCCGAT 
GAGAGCCACT TCGCGGTGTT TGCCCCCTCC CAGGCGGCCA TGGACGAGGC CAAGGAGCTC 
ATCGAGGGAT ACATGGTCAA GGAGAGGATA CCCGACCTCG AGTTCGGGGG AATATACACT 
GCCAAAGTAA CCGAGCTGCG GGACACCGGC GTCATGGTCA TCCTGTATCC CAGCATGCCG 
CCGGCCCTGC TGCACAATTC GCAGTTGGAC CAGCGAAAGA TAGGGCATCC CTCGGCCCTG 
GGCCTGGAGG TGGGCCAGGA CATACAGGTC AAGTACTTTG GCCGCGATCC CGTCTCCGGC 
TTCATGCGGC TCTCCCGAAA AGTGCTCCAA GGCCCGGCCC TGGGCATTCC GCGATCCCTA 
AACAAGGCGG CCGGCGAGAA TGGCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001357946.1
CDS	158..2467
Translation	MIFTRKTLKLLNYRLKSIAVARLSAHRCLQSSASGEVPSVEVKFSNGRNMSISSGKLARFANGTAVCQMGDTAVMVTAVAKPKPTPGQNFMPLVVDYRLKNAASGRIPMNFMRRELGPSEKEILSARLIDRSLRPLFNKDYRTETQLVCNMLAMDAVHSPDILAINAASMALSLSDIPWNGPIGAVRVGLSDGEVLINPTRRELQSSQLDLVVSATKQNLVVMLEGKGNVVLMQDLLKAIKQGTREAQFIIQEIERLQKAYGRKKREVESPNRVDPELQEAVRSMCEMRLREIFQDANHDKISRDNAVNEVRSNVIDKVWSSYPDTEPGVIGEEFNQTCRSIFRELIFERNYRCDGRDYDTLRNISCQVDMYKPLHGSALFQRGQTQVFCTVSLDSPESAMKLDSLAALDSGGLKAKNFMLHYEFPPYATGEVGRIGPLGRRELGHGALAERSLLPTLPHDYPFTVRLTSEVLESNGSSSMASVCGGSLALMDAGVPVSAPAAGVAIGLVTKYENDDTKHLQDYRILTDILGIEDYMGDMDMKVAGTRKGFTAIQADLKVPGIPLKVVMESLQKATDAKSKILDIMAEAIREPRKYPKDSWPVSETISVEPHQRSQLIGPSGLHMKRIYLETGATLTAADESHFAVFAPSQAAMDEAKELIEGYMVKERIPDLEFGGIYTAKVTELRDTGVMVILYPSMPPALLHNSQLDQRKIGHPSALGLEVGQDIQVKYFGRDPVSGFMRLSRKVLQGPALGIPRSLNKAAGENGT

Target ORF information:

RefSeq Version	XM_001357909.4
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA10930), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001357909.4

ORF Insert Sequence:

ATGATTTTCA CGAGGAAAAC ACTGAAATTG CTAAATTACC GCCTGAAAAG CATTGCCGTC 
GCCCGCCTAA GTGCCCATCG ATGCCTCCAA AGCAGCGCCA GCGGAGAAGT GCCCTCCGTG 
GAGGTGAAGT TCAGCAATGG ACGTAACATG AGCATCAGCT CGGGGAAGCT GGCGCGCTTT 
GCCAATGGCA CTGCCGTCTG CCAGATGGGA GACACCGCCG TGATGGTGAC AGCGGTGGCC 
AAGCCGAAGC CCACTCCCGG CCAGAACTTT ATGCCGCTGG TGGTGGACTA TCGCCTGAAG 
AACGCCGCCT CGGGGCGAAT TCCAATGAAC TTTATGCGCC GCGAGCTGGG TCCCTCCGAG 
AAGGAGATCC TGTCGGCCCG CCTGATCGAT CGCTCGCTGC GTCCGCTGTT CAACAAAGAC 
TACCGCACGG AGACGCAGCT GGTGTGCAAC ATGCTGGCCA TGGACGCGGT GCACTCGCCC 
GACATTCTAG CCATCAATGC CGCCTCCATG GCCCTGTCCC TGAGCGATAT ACCCTGGAAT 
GGTCCGATTG GAGCTGTGCG GGTTGGCCTT TCCGACGGCG AGGTGCTGAT CAATCCCACC 
CGCCGGGAGC TGCAGTCCTC GCAGCTGGAC CTCGTGGTGT CGGCCACCAA ACAGAACCTC 
GTCGTGATGC TCGAAGGCAA GGGCAATGTT GTGTTGATGC AGGACCTCCT GAAGGCCATA 
AAGCAGGGCA CGCGGGAGGC ACAGTTCATC ATCCAAGAGA TCGAGCGCCT GCAGAAGGCC 
TACGGCAGGA AGAAACGCGA GGTGGAGTCC CCGAACCGAG TGGATCCCGA GCTGCAGGAG 
GCGGTGCGCA GCATGTGCGA GATGCGGCTG CGAGAGATCT TCCAGGATGC GAATCACGAC 
AAGATCTCGC GCGACAACGC AGTCAACGAG GTGCGCTCGA ATGTGATCGA CAAGGTGTGG 
TCCTCCTATC CGGACACCGA GCCAGGGGTG ATCGGCGAGG AGTTCAATCA GACGTGCCGC 
TCGATCTTCC GGGAGCTAAT CTTCGAGCGG AATTACCGCT GCGATGGCAG GGACTATGAC 
ACGCTGCGGA ACATCTCCTG CCAGGTGGAC ATGTACAAGC CGCTGCACGG CTCGGCCTTG 
TTCCAGCGCG GACAGACGCA GGTCTTCTGC ACCGTGAGCC TGGACTCCCC GGAGAGTGCC 
ATGAAGCTGG ACTCGCTGGC CGCCCTCGAC AGTGGCGGCC TCAAGGCCAA GAACTTCATG 
CTCCACTACG AGTTCCCGCC GTACGCCACG GGGGAGGTGG GCAGGATTGG CCCGCTGGGT 
CGGAGGGAGC TGGGACACGG CGCCCTGGCG GAGAGATCTC TGTTGCCCAC ATTGCCACAC 
GACTACCCCT TCACGGTTCG CCTCACCTCC GAGGTGCTCG AGTCGAACGG TTCCAGCAGC 
ATGGCCAGCG TCTGCGGCGG ATCCCTGGCC CTGATGGATG CCGGGGTGCC GGTGAGTGCC 
CCTGCCGCGG GGGTGGCCAT TGGTTTGGTC ACCAAGTACG AGAACGACGA CACCAAGCAC 
CTGCAGGACT ATCGCATTCT CACCGACATC CTGGGCATCG AGGACTACAT GGGGGACATG 
GACATGAAGG TGGCGGGCAC ACGCAAGGGA TTCACAGCCA TCCAGGCGGA CCTCAAGGTG 
CCTGGCATTC CATTGAAAGT GGTGATGGAA TCCCTGCAAA AAGCCACCGA TGCCAAGTCC 
AAAATACTGG ACATCATGGC CGAGGCCATT CGAGAGCCAC GGAAATACCC AAAGGACAGC 
TGGCCCGTGA GCGAAACCAT CAGTGTGGAG CCGCACCAGC GCTCCCAGTT GATTGGCCCC 
AGTGGCCTGC ACATGAAACG CATCTACCTG GAGACGGGCG CCACCCTCAC AGCCGCCGAT 
GAGAGCCACT TCGCGGTGTT TGCCCCCTCC CAGGCGGCCA TGGACGAGGC CAAGGAGCTC 
ATCGAGGGAT ACATGGTCAA GGAGAGGATA CCCGACCTCG AGTTCGGGGG AATATACACT 
GCCAAAGTAA CCGAGCTGCG GGACACCGGC GTCATGGTCA TCCTGTATCC CAGCATGCCG 
CCGGCCCTGC TGCACAATTC GCAGTTGGAC CAGCGAAAGA TAGGGCATCC CTCGGCCCTG 
GGCCTGGAGG TGGGCCAGGA CATACAGGTC AAGTACTTTG GCCGCGATCC CGTCTCCGGC 
TTCATGCGGC TCTCCCGAAA AGTGCTCCAA GGCCCGGCCC TGGGCATTCC GCGATCCCTA 
AACAAGGCGG CCGGCGAGAA TGGCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA10930 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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