Dpse\GA13781 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA13781
Entrez Gene ID	4800269
Full Name	GA13781 gene product from transcript GA13781-RC
Synonyms	GA13781,dpse_GLEANR_4807
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015183061.1	XP_015038547.1	uncharacterized protein, isoform B
XM_001357584.4	XP_001357621.3	uncharacterized protein, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA13781 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA13781 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe21125	XM_015183061.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA13781), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODe18962	XM_001357584.4 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA13781), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA13781 ( XM_015183061.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015038547.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA13781), mRNA.

Price & Availability↑

CloneID	ODe21125
Clone ID Related Accession (Same CDS sequence)	XM_015183061.1
Accession Version	XM_015183061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCAAAC TAGAGTCCTA CATCACCCCG ATTCTGCTAA GCTACGTGGC CAAGTATGTG 
AAGAATTTCC GTGACGAAGA TGCCCAGGTG TCACTGTGGG AGGGTGAGGT GACGTTTCAG 
AACCTCGACC TGCGCCTGGA GGTGCTGGAG GAGGAGCTCA ATCTACCCGT GGAGCTCGTT 
TCGGGGCACA TCCACGAGCT GAGCATCCAG GTGCCATGGA CAAAGCTCAT GTCGGAGCCG 
GTGAAGATTA TCATAAACAC AATTGAGTTT GTGGCCAAGC TGCCGGACAA TGAGAGCAGG 
CAGCGTCGTG CCTCGTACCA AAAGGAGCAG CGCCGGAAGG CGAAAAAAGC CGCAGTGGAG 
GCTGAACAGC AGGGTGCCGC CCCCGCCAGT AGCTCAGTCG TGGTGAAAAA GATAATCAAC 
AACATCACCT TGCAGTGCCA CAACATCATT CTGAAGTACG TCGAAGACGA CATTGTCGTG 
TCCATGAATG TCCAGTGCAT GAACTTTAGC CCCGCCGGCG AGAACTGGAA GCCCACAATG 
GTGGACGTGC ACCCTGTGTC GGTTTCCATG CGCAAACTGC TGCAGATATC CGATCTTACC 
ATCTGCCTCG ATAAGCGGAA CACGGCCGGA AGGATTGAGG TGTGCCAGGA GCCGGTCCTC 
TATCGCTGCA CCCTGGAATG TCGCGTCCTG CGCAAGTACA ATGCCACCAC AGTCAGCACC 
TCGAGCACGA CCAGAATCGG GGTCTTCACC AAAGCGTTGG ACATTAACGT GTCCTCGCTG 
CAATTCCCGA TGGTCATGAG GTTGGTCAAA ATGCTGCTGG AACTCAAGCC TGCGGAGATG 
GAGGAGGAAG CTCTCAACCC AGACGATCCA GAGGAAACAG CGGAGGGCAA CGAAGTGCCG 
GTGGGGGGTA CACAGCCCGA GGGTCAGAGT TATCTGTGGT GGGCCTGGAA CCTTTTGCCC 
AGCTTCGAGT CGCAGGTCCC CGACCCCGAC CCAGAGGAGA CCATTGGCCA TGCCTTCGAT 
ATAGGCATAT ATGCTGAGGA GCTGAACTTT CAGCTGAAGA ATTCGGAGTT CTTCACCGAT 
CAGGCCATGG GAGGGATAAA GCGGATCCGC TATACACCCA TCCTGCGCAT CAGTTTGGGC 
GGCATCTACT ACGAGAGATC GCAGTTGAAG GAGAACGACT GGACGAACGT CAAGGCGGGG 
CTCTCGAGCC TTTGCATGGA GCCCTTGGGG GCATACAGGA GCGAGGATCC AGTCGATCGG 
GTGTTGGTCA ATACCCAGGA GTCTTTAAAG GGGCGCACAT TCATCGACAA GAGCCTGTTC 
GACGAGCACT TTATGTTCTC GGATCGCACC TGGTGCCGCC ACAACTACGA GGACTACGTG 
GCCAGGAACA CAGATGAGTA TATGCTCTTC CGCAGCCCCG TGCTGTCGTT CGATATTGTT 
GAGTACCGGG CCCCAAAGCC GAACAGCCAT CCCCTGGCCG AGAGCCAGCT GAAGGACCTG 
GGACTGCGCG TTCAGTATCG TCTACTATCC GCTGGGATTA CTTTCCACTT CTCACAGTCA 
TTTCTCCAGA GTCGGTCAGG GAGGAGCCCC AGCACGAGGA GCACTTTAAC ACAGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015038547.1
CDS	150..1769
Translation	MFKLESYITPILLSYVAKYVKNFRDEDAQVSLWEGEVTFQNLDLRLEVLEEELNLPVELVSGHIHELSIQVPWTKLMSEPVKIIINTIEFVAKLPDNESRQRRASYQKEQRRKAKKAAVEAEQQGAAPASSSVVVKKIINNITLQCHNIILKYVEDDIVVSMNVQCMNFSPAGENWKPTMVDVHPVSVSMRKLLQISDLTICLDKRNTAGRIEVCQEPVLYRCTLECRVLRKYNATTVSTSSTTRIGVFTKALDINVSSLQFPMVMRLVKMLLELKPAEMEEEALNPDDPEETAEGNEVPVGGTQPEGQSYLWWAWNLLPSFESQVPDPDPEETIGHAFDIGIYAEELNFQLKNSEFFTDQAMGGIKRIRYTPILRISLGGIYYERSQLKENDWTNVKAGLSSLCMEPLGAYRSEDPVDRVLVNTQESLKGRTFIDKSLFDEHFMFSDRTWCRHNYEDYVARNTDEYMLFRSPVLSFDIVEYRAPKPNSHPLAESQLKDLGLRVQYRLLSAGITFHFSQSFLQSRSGRSPSTRSTLTQT

Target ORF information:

RefSeq Version	XM_015183061.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA13781), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015183061.1

ORF Insert Sequence:

ATGTTCAAAC TAGAGTCCTA CATCACCCCG ATTCTGCTAA GCTACGTGGC CAAGTATGTG 
AAGAATTTCC GTGACGAAGA TGCCCAGGTG TCACTGTGGG AGGGTGAGGT GACGTTTCAG 
AACCTCGACC TGCGCCTGGA GGTGCTGGAG GAGGAGCTCA ATCTACCCGT GGAGCTCGTT 
TCGGGGCACA TCCACGAGCT GAGCATCCAG GTGCCATGGA CAAAGCTCAT GTCGGAGCCG 
GTGAAGATTA TCATAAACAC AATTGAGTTT GTGGCCAAGC TGCCGGACAA TGAGAGCAGG 
CAGCGTCGTG CCTCGTACCA AAAGGAGCAG CGCCGGAAGG CGAAAAAAGC CGCAGTGGAG 
GCTGAACAGC AGGGTGCCGC CCCCGCCAGT AGCTCAGTCG TGGTGAAAAA GATAATCAAC 
AACATCACCT TGCAGTGCCA CAACATCATT CTGAAGTACG TCGAAGACGA CATTGTCGTG 
TCCATGAATG TCCAGTGCAT GAACTTTAGC CCCGCCGGCG AGAACTGGAA GCCCACAATG 
GTGGACGTGC ACCCTGTGTC GGTTTCCATG CGCAAACTGC TGCAGATATC CGATCTTACC 
ATCTGCCTCG ATAAGCGGAA CACGGCCGGA AGGATTGAGG TGTGCCAGGA GCCGGTCCTC 
TATCGCTGCA CCCTGGAATG TCGCGTCCTG CGCAAGTACA ATGCCACCAC AGTCAGCACC 
TCGAGCACGA CCAGAATCGG GGTCTTCACC AAAGCGTTGG ACATTAACGT GTCCTCGCTG 
CAATTCCCGA TGGTCATGAG GTTGGTCAAA ATGCTGCTGG AACTCAAGCC TGCGGAGATG 
GAGGAGGAAG CTCTCAACCC AGACGATCCA GAGGAAACAG CGGAGGGCAA CGAAGTGCCG 
GTGGGGGGTA CACAGCCCGA GGGTCAGAGT TATCTGTGGT GGGCCTGGAA CCTTTTGCCC 
AGCTTCGAGT CGCAGGTCCC CGACCCCGAC CCAGAGGAGA CCATTGGCCA TGCCTTCGAT 
ATAGGCATAT ATGCTGAGGA GCTGAACTTT CAGCTGAAGA ATTCGGAGTT CTTCACCGAT 
CAGGCCATGG GAGGGATAAA GCGGATCCGC TATACACCCA TCCTGCGCAT CAGTTTGGGC 
GGCATCTACT ACGAGAGATC GCAGTTGAAG GAGAACGACT GGACGAACGT CAAGGCGGGG 
CTCTCGAGCC TTTGCATGGA GCCCTTGGGG GCATACAGGA GCGAGGATCC AGTCGATCGG 
GTGTTGGTCA ATACCCAGGA GTCTTTAAAG GGGCGCACAT TCATCGACAA GAGCCTGTTC 
GACGAGCACT TTATGTTCTC GGATCGCACC TGGTGCCGCC ACAACTACGA GGACTACGTG 
GCCAGGAACA CAGATGAGTA TATGCTCTTC CGCAGCCCCG TGCTGTCGTT CGATATTGTT 
GAGTACCGGG CCCCAAAGCC GAACAGCCAT CCCCTGGCCG AGAGCCAGCT GAAGGACCTG 
GGACTGCGCG TTCAGTATCG TCTACTATCC GCTGGGATTA CTTTCCACTT CTCACAGTCA 
TTTCTCCAGA GTCGGTCAGG GAGGAGCCCC AGCACGAGGA GCACTTTAAC ACAGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA13781 ( XM_001357584.4 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001357621.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA13781), mRNA.

Price & Availability↑

CloneID	ODe18962
Clone ID Related Accession (Same CDS sequence)	XM_001357584.4
Accession Version	XM_001357584.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001357584.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCAAAC TAGAGTCCTA CATCACCCCG ATTCTGCTAA GCTACGTGGC CAAGTATGTG 
AAGAATTTCC GTGACGAAGA TGCCCAGGTG TCACTGTGGG AGGGTGAGGT GACGTTTCAG 
AACCTCGACC TGCGCCTGGA GGTGCTGGAG GAGGAGCTCA ATCTACCCGT GGAGCTCGTT 
TCGGGGCACA TCCACGAGCT GAGCATCCAG GTGCCATGGA CAAAGCTCAT GTCGGAGCCG 
GTGAAGATTA TCATAAACAC AATTGAGTTT GTGGCCAAGC TGCCGGACAA TGAGAGCAGG 
CAGCGTCGTG CCTCGTACCA AAAGGAGCAG CGCCGGAAGG CGAAAAAAGC CGCAGTGGAG 
GCTGAACAGC AGGGTGCCGC CCCCGCCAGT AGCTCAGTCG TGGTGAAAAA GATAATCAAC 
AACATCACCT TGCAGTGCCA CAACATCATT CTGAAGTACG TCGAAGACGA CATTGTCGTG 
TCCATGAATG TCCAGTGCAT GAACTTTAGC CCCGCCGGCG AGAACTGGAA GCCCACAATG 
GTGGACGTGC ACCCTGTGTC GGTTTCCATG CGCAAACTGC TGCAGATATC CGATCTTACC 
ATCTGCCTCG ATAAGCGGAA CACGGCCGGA AGGATTGAGG TGTGCCAGGA GCCGGTCCTC 
TATCGCTGCA CCCTGGAATG TCGCGTCCTG CGCAAGTACA ATGCCACCAC AGTCAGCACC 
TCGAGCACGA CCAGAATCGG GGTCTTCACC AAAGCGTTGG ACATTAACGT GTCCTCGCTG 
CAATTCCCGA TGGTCATGAG GTTGGTCAAA ATGCTGCTGG AACTCAAGCC TGCGGAGATG 
GAGGAGGAAG CTCTCAACCC AGACGATCCA GAGGAAACAG CGGAGGGCAA CGAAGTGCCG 
GTGGGGGGTA CACAGCCCGA GGGTCAGAGT TATCTGTGGT GGGCCTGGAA CCTTTTGCCC 
AGCTTCGAGT CGCAGGTCCC CGACCCCGAC CCAGAGGAGA CCATTGGCCA TGCCTTCGAT 
ATAGGCATAT ATGCTGAGGA GCTGAACTTT CAGCTGAAGA ATTCGGAGTT CTTCACCGAT 
CAGGCCATGG GAGGGATAAA GCGGATCCGC TATACACCCA TCCTGCGCAT CAGTTTGGGC 
GGCATCTACT ACGAGAGATC GCAGTTGAAG GAGAACGACT GGACGAACGT CAAGGCGGGG 
CTCTCGAGCC TTTGCATGGA GCCCTTGGGG GCATACAGGA GCGAGGATCC AGTCGATCGG 
GTGTTGGTCA ATACCCAGGA GTCTTTAAAG GGGCGCACAT TCATCGACAA GAGCCTGTTC 
GACGAGCACT TTATGTTCTC GGATCGCACC TGGTGCCGCC ACAACTACGA GGACTACGTG 
GCCAGGAACA CAGATGAGTA TATGCTCTTC CGCAGCCCCG TGCTGTCGTT CGATATTGTT 
GAGTACCGGG CCCCAAAGCC GAACAGCCAT CCCCTGGCCG AGAGCCAGCT GAAGGACCTG 
GGACTGCGCG TTCAGTATCG TCTACTATCC GCTGGGATTA CTTTCCACTT CTCACAGTCA 
TTTCTCCAGG TTAAGAATGT AATTAGCGAT TTATTGCGTC CTTACGATTA CTCGGGATAT 
CATTCAGAGT CGGTCAGGGA GGAGCCCCAG CACGAGGAGC ACTTTAACAC AGACATGACC 
CTGGCGGACT TGGAGTACCT CATTGGATTC GCTCCCACCT GCAACTACAA GATCGAGCTG 
CGGAACATGA CCATTCAGCT GTATCCGCGC CAGCAGCCAA AAGAGTCCTC GGCAGCCAAT 
CAGCATCAGC TGTCGACCAC GGTCAAGCAA TCCCTGCTGC CATATCTCCA GCTAAGACTA 
CCCCTAGTCG AGGGCACGCT GTGCAAGCCA GTGAACGCAC ACCGTCTGGT CCAGTTGATC 
ACCCAGCTGC AGGACAAGCC GCGAGAGCTC CTCAATTCGT GCTACAATTG CTACAGATTC 
AGTGTGAAGA ATATCACGCT GATGATCCTA AATACCACGC CCGAGAACGG CCGCGCAAAG 
CTGCTGAACA TTCCCCGGAT GCAGTTGAAC GTTAATCGTC TCCTGCTCCC CCATCTCTGG 
CGACCCAACG ATGCTGCGCT GGAAACGGCG GAGATTCAGT CGGAGATCCT GACTATAGAG 
TTCTCGAAGC GCGAGCTGAC CCTCGCCAAG CGCATCATTC CGCTGATAGC GAGCTTCAAC 
GGCACAGAGC TCTGTGCTGT GTCCCATTTT GTGGCGCAAT CCACTGCCAG TTCGGATGTG 
ATCAAGCTGC AAACGCTGGC CACAAAACTG CGACTGAATT TCCACAAGTA TCACACGCAT 
CTATCGGCAA ATGCGTCGCT GCGAAGCCTC AACACAGATG CCTACCACAC GAAGATGAAC 
ATGCGCAACG TGGTGCTGTC CACCACCAAG AACCTGAACA ACAAGTGGCT AGAGGTGCAG 
CTGCAGCTGC CACTGCCACT GCAGGAGACA GCCTTGCAGC AACAGGAGCA GATGCCCGGG 
ACGGTTTTGT GTCTGTGGGT GGAGCATTGT CGCTTTACCA TAGATATATA TCTCCTGCAG 
TTCCTCAAGT TCCCAGAGCA CACAACGAAA AGGAAATCAA CGGAAAATGA GATCGAAATG 
GATGTAGATG CTGCCGCAAG CCAGTCCGAG CATCATTTTC CTCAATCGGT GTCAAACTAT 
TCCACGATAT CTGCCACAGC GTTGCCCCTT CAACCGCGTC GAATCAGCCG CAACAATAGC 
CGAAAGATCT CCGTCCCTGA GGAGACAGTG CATCTCAGCT CCGAGCGGGA TGAGAGGCAC 
ACACAGATGG AACCAGTAAC CGGTTTATTG ATGGACAGCA AGCCGCAGAA GACTGCTCTA 
GATCTTGGTC AGCTGGCGAA GCGTCTCGGC TCCATGGTAG TGCTCGCAGA GATTGCCCAG 
ATCCGGGTAG ACGTGTGCGA GCTCATGGTG CGCAGGAGGC CCGCAGAGTC GGATGTGGAA 
TACACGTCCA TACGGCTGCC CCGCATGAAA ATCAAGTCCA GCAACTGCGA TTTCGTTTCC 
CGGGGGAACA TCAGGAGTGT CATTTTGGTG GATTCCGAAG CAGATCTGGT CAACTGGGTC 
ACGGATCTGA ACGACTTGAA TGTCTGCTAC TGCAGGGGAG ACATATCAGA AGAGGTTTTG 
GCACCAGTCA GGACTACCAT CACTCTGGCC ATGTCCTATA AGCCAATCGA GGAGAAGCTT 
CCGGGATCCC TTCATTCCGA GAAGACGGCC GCGGTGGATC AGAAGCAGGA CCAGACCTCC 
GATAAACCTG TAGAACAGCG AGGCTTTGGT ACTTACTCAA TAAATGTGCA CGTGGACATG 
TCGCCCATTA ATGTTTTTGC TCGAAGGGTG AAACTTCTAC ACGAGCATTT TCAAATCGTT 
TCCGAGATTC ACAGATCTCT CAGTCCTGAA GAGGATGGCA ATGTCAAATG GAGCTCGGGT 
CCCCGACTGG AGGTTTATAC GGGCAGCTCC CAAACCTATC CCGTGATAAA GGAGTTTCTG 
GAATTGGATC CCAACTTTGA GCCCCCTCAA GCGAAAAATG TTCCCAATTT AGATGTCTCG 
CTGATATTCT TCTGCCAGTG GACCATACCA CGGATCAGCT TCGAAATGGA GAGCCCCACC 
GATCGCAAAC AGAACAAGGT TGTCGTGACT TTCGAAGATC TACTACTGAA CGTGGACAAG 
CATTCGGACT TTACGAAGGT CACCACCAAA GTGGAGAACC TGGGACTCAA CTACTACGAG 
GCCAAGCCGC ACGGGGAGTG GCTCCAGAAG GATGGCTTCT ACGTCAAAAT GCTGGGCGAC 
AGCGGCAATT TGCCGCTGAT TAGTGTGGTG GTTACCACCG TCAGCCTGCA GGATCTGTAC 
TCGAAGATTG GAGCCAGAAA TCCCCGCAAT AGGCAGCACA CAATTTCTGA GCTGCTGATC 
GAACTGCAGC CCATTGAAAT GGTCCTCGAC CTGGATCAAA TCACAGACTT TGTGATTCCC 
CTGTGCGGAG TGATAGAAAT AATGAGGGGC AATCATCAGG CACCAGCTAT TGAGAGCCGC 
TTAACCACCG TTCAGGACTT GCCCATGGTG CATCTGAACA GCAAAGGAAT CTATATCTAC 
CTGCCATTGG CCACGGGCAG AAAGTGCTGC AGTGTCTTGT TGCTAAGGAT TGAGACTGTG 
CAGCTAACGC CTAGCCTGGA GAATCCTTTA GTGCGGCAAC TGGTTCGCCA GGACATCTAC 
CAGAAGGCCG CCGAGCTGAA CATGCTGAGC ACCCCCGGAG CCCTGGTCGA GGATCGTCAG 
TACGAGCTGA GCGTCAGAAA TGTGTCCCTG TCTACTGGGA ACTGGCTGCA GACCCAAAAG 
TATCACATGG AAAAGAGTCT GGCCAGTGAG CACAACAATC CGGCCTTCGA GTGGAACAAC 
CAGACACGTC CCACTGAGCT GGACCACATG GATATATTCA GGGACTTCGA CTTCATCATG 
GTCTATGCCC CGGCCATTAT CTACGATCGG TTTTTCGTCT GCGGCCAGAG TGTGGAGTAC 
AACTGTGTGA CGGACTTTGT GGCCACTCTG ACCACACACC AAATCAATCT GATATCCTGC 
ATCCTAGTGC GTTTTCAGCG TTTGTACAGT GTCCTCGATC AGATGTATGC CAAAGAGTCG 
CCTTCGCTGA CGGAGCTGTC ATCACAGTCA ACGCACAGCA TCACGTGCAC CCCCGTGGAC 
ATTTCGGCCA AGAGCAGTCG GCTAAGACAC TCTGGCCATT CGGTCCGGTC CAGCGCCAAG 
CGAAGTCGTT CCAGTTTCGT GGATGCCAGG AGCAGCAGCG AAGTCTTTGT GGGCTCCTGC 
CAAAGTGATT CCGGAATACA CGTGGGAGGG GGTGGTGTAA AGGGAACTCA GAAATCGCAG 
GAGGAGCTGC AACCGAGTGG CGTACGCTAC CCGCAAAGTG TCTCCTTTGT GGCTGGGATA 
TTTGTGCTAA AGATCTACGA AGTCCTGGAG CTGGAGGAAC TGGAGCAGCC AGTGATGAAG 
CCGCTCATTC TGCTGACAGT CTCGCAGCCC AGCTATATGT GCACCCAAAG TCTGAAGGCA 
ACCATCACGC AGGCATCCAT TTTCAATCTA AACGTAACTG TGGCCACTCC TGGTACGGAC 
CCTTCGGTGG ACCCCTTCAA TGAGTCTGTG TTTGACACCC TGCCAGGTAC TCTCAGCAGT 
TCGGGGATTC CCCCACCGCT GCTCACTGTG CGGTCCCAGT GCGATCGCAT CCATCAAACC 
GAAGTGGATG TGGAGGTGCG CAAGCCCATA GTGCTGCGCC TGTGCGAGAC GAGCATCCAG 
CAACTGGTCA ACAATGTCAT CCGCATCTAC AACGTGCTCC TCGAGACTCC CTGCTTCGCG 
GTGCGCAGCG AGCGGCCTGT GGTCCAGGGC ACACAGTTGC GGCAGATGAA GAACCACTGC 
TACAATGCGG ACCGATTGAA TCTGTCCTTT GATCAGGTCA CCATCACGCT CTGCGACGAG 
GAACGGTCCT ACAAATGCAG CTCCGTTTGG CTGGACTTTA GCACCAGCAT CAAGTTCAAT 
CCGCGTCCCC AGAAGGCCGT CATTAGATCG ACGATTGGCT CCTTCTACAT CCAGGCTGGC 
GAGAAGATCT TCCTGCATCC CCTGTTGATG CGTTTGTCCG TGGATCTGGT CAGCGAGCCA 
TGGTGTGACG ATCTCCTGAT CAACGCTACT CTCAAGCTAC ACTTTCTGCA CGTCGATGCG 
AGTGTTTGGA GTATTCTGCA ACTGCAAAAG GCCCATGAAG GTCTCGGCCG GATCCTTGAG 
TATGCCCATC AGGAGTGGCA GGGCTTTCTG GAACATCGTC CGGCAATGGG CACTCCAACA 
GAGATTGCAC CCGGAGCGCT GATCAAGTGC AATCCATCGG TCGACTCGAT CGGGCGCTGC 
AAACGCTCGC TGAAAACTAA AGCAGAATTC TATCAGGACG ACCTCAGGGC GGGCGCCTTC 
CAATTTGTCT ACTTGAACTC CGAGTCCGTG CTGCCTATGC CATACCAGGT GCAGATCATC 
AAGAAGAACT ACGGGATTAT CTGCTGGCGC TATCCACAGC CACGCCAGAT GACCAGCATC 
CACATCTATC CGATTCCCAT GCCAGTCTCA AACCCCATAC ACATCAAGTG CAGCATGGAG 
TACTTCAGTG AAACGCACGA GACTTTTCTG CACTACTGCG ACTTCCAGCT GTCGGAGACG 
GCCACCAAGC AGCTATCCCC ACAAGATCGT CAGATCTGTG CGAACATCTG GAGAGTGGTC 
ATTCTGCAGT CGCTGATTTC TGTCGATGGC ACCTGCTTTG AAGGAGATGA GGATGAAGAT 
TTCCATTCTA TCGACAGCTG TCGCATTGAG CAGGCCTTTA AGACCGATGC AGACAACGAT 
TTCATATTGC ATCCAAAGGT TCTTGTGGGC TGCATGCGCA TCGATACCAT CTTCAGGGCA 
GAGACAGTGC CCAGGCTGCA GCTGCTGCTC TGTTGCCAGG ACATGGAAGT AAACCTTCTG 
AATCAGCCGG ATGGCAAGGG GGAACTGCCT TCGCTTTTGA AAGAATACTC CCCCAAGCGG 
TCAATAGACT CCCCACAGAC CTTTCTCACA GTGCACGTGG GAGATCTGCA GGTACACTCG 
GCTGTCTATG CCAGGAATGA CTTCAGTCTG GAGACGGACT TCCGCGCGCG CGTGAAGTGC 
TTGGACTATG GCTTCCTCAA CATGCTGGAC ATCGTGGAGC CTATGAAATT CTCCAGCTAC 
GCGATGTTCA CCCACTCTCC GCGCTCGCTG AAAGCGAACT TTCTGCTGGA CAAGCTCCGC 
TTCAACTGCG GTCCCTACAT GATCCACACT CTGCTCAGCT CCAAGCATCA CTGGCTGGAA 
GTGCTGCGCC AGAGCGAGAT CCGTCACACT TTAATGCCCA AGTGCGTGGT GGTCAACCGC 
ATGCAGGCGG GCCTAAGCTT CGGGCAGACG GGCACGAACG AGCAAATATC CCTGGCACCC 
CATGAGCTGC AGCTCTACCA CTTCCGCAGC TGTGCGCAAG GGCAAGAGCT GACGTTCTTC 
ATTCGGAATC CAGAGACCAA GCAGATGGAG GCCAGCAGCT CGGTGCCCAT AGCCCTGAAA 
TTCGAGGAGG AGCAGAGAGT GCGTCATCTG CGGGTGGGAA AGTGTTGCAT CACAATCAAG 
GTGGGAAAAC TGTCCGCCAC GCAGATCTAT ATACTGGTGA AGGGCCAGAT TGAGCTGATC 
AGCATGGTGC CCTTTGAATT GATCACCGAA TTCCGCCAAG AGGGAAAACG CTACGACGAG 
AATACGAGAC CACACGAGCA CCTGCTTTTG GCCAGGGGAA GGGCATCCTT CTATCATACG 
GTGGTGCGTA ATGGAGACAT TAACTTGCGT CTTAAGTTAG CGGCTGGTCT CGGCAAGGGA 
CGCACTGGAG ATATTCCTTT GAAAACTAAC AATAATCTGC CTTGGCTGGT CAAGGTGCCC 
ATGCAAAACC CCGTCCAGCA GTTTGTCAGC GTGTGGGTGC GCATCCTGCG CGAGGATGTG 
GATTTGGACT CAGCCGAACC GGACTTTCAT CCGCAAAAGA TATTGATTTG CATTTGGCCC 
ATCTTCGAGA TATGCAACAT GCTGATCGAC GATCTTCAGG CCACAGAAAG TACGAGTGGG 
CAGAGCTCCA AGATCACAGC CCATGGCGGA AGGATGTCTC TGCAGACGGC TACCACGCAC 
AGCACAGAGC ATCGCGTTGG CTTTCAGTTT CCGGCTCCCT TGTGCAGCAC CTCCCAGGAG 
GATTATACAC TCATGCTCAA GACCATGGAC TGGCACAAGT TCTTTTACTT CGATCCCGCT 
CAGTGGACGA TTGAGAACTC GCTGCACAAA CTGGGGAAGA CGGTGAAGCC CAAGTGGCCG 
CTGAGCGACG ATGAGGAATC GCGCGTGCTT CGCCGTGTGT CACTGACCAA CGCATTCGAT 
GTGGAATACC GCTTAAAGGC CACGCGGGAG TTTTCCTGCA CTTTGGGATT GGAGGTTTCT 
GCCTGGGGAA TGCTCATCAA TGCCACCGGT CTGGACCTGA GCATCTGCCA GACCAGGGAC 
CGCGAGCGCC ATCAACTGAA CGCCAACTGT CTGGAAATGG TGCCCAAATT GTGCGGTCTG 
TTCACAATTG ATGTGCCTTT TGGCTGCAAT TGGATTGCCT CGACGCCCAT TTACCTGGAG 
GAACTGACCA AGACAGTGGA GAACAGCCCC AAGCGGAGCA TGATGCTCCG CTGTAATTCC 
TTTGTGGACA TCGTGGTGGT GCGAAACGAG GAGGTGCTGC GAATGATGCT GGAGTATAGG 
CTGGAGGATG GGCGCCGTGT ATTTAAGCTG CGCTCCAAAT TCGTAATCAC CAATTTCACA 
GATGTCTCTC TGAATGCCCT GCCCTTGACC ATGGATCACA AGGAGACCTC CACAAGGGAG 
GAAGTGAATG CGTTCAGCAT GGCCAACAAG AGTCGCAGTC TCGTATCTGT GGATGGGGCA 
AAGAATTGCG TTGGAGTGAC GATGGACGTT TTCTACGATC TTAATGCCCG TGCGGCTAAT 
CATACCTGTG ATACGGCTTT CGTCTACTTT CTGTGCTTCA GTGTGGCTGG TAGTCGCGAC 
ATATCTATAC CCATTCCCCT GGCCATACCC TTCACGAGAC GCTGCTTCAG CCTGCAGTCT 
GGACGGGAAT CGGTGCCATT CATGATCACT CTCATTGAGA AGGACAATGT CCATTATCTG 
AATATATTCC GCGATACGGC CCCGGCACTG ATCATCAGCA ACAGCACGAA TGTGAAGTTT 
ATTGTGGCCC AGACGAGTGC CTCCGGCAAC AGCAATGTGA CTTGCACAAA CTCCGAGTTC 
GTAGGGAGGC ATTTTGAATG GAATCAGTTG GTCCCGCCGA ACAGTAAATG TTACTACACC 
CCGCCGCAGA TGTACGCCAA CTTTCCCGAT GTGGAGTTTA CCATGTGTAA TCTGTCGTTA 
GCTGTATATA ATGAAAAGTC CTGTGGCAAG AAGAAACTCG GCTGGTCAAA GCCCATAAGG 
ACCGACAAGT CGTGGAAGAA GTTCTTGCAT GTGCCGAATC ATGGCGAAGT GAAGGTGGTC 
GTCTGTGATA AGCATCGCGT CATACGCGTC AACATCTACT ACATAACCCA GCAACTGGAG 
TTTTCTGTGA AGGATCTACG CTCACGGTTG ACAAAGCCAG AGAATCGTTT GCTGCCCTCA 
GAGCAGGATT CGGAAAGACA GTTGGACGAG ATCGAGACTG TTACTGCTAC GGCGTCAGCT 
ACGGCTACTG ATGCCAGCGA TCCATTGCCG GACATTGTGG GCTGTGCCCA TTTTAGCGAG 
GAATGCGAGT CCCCGCCACA GATCAAGATA CGAATATTCG TGAAGCGCTT TGTGTTCAGC 
CTCCAGACGG ACAGTCGGGA GAAGGACTAC TTAAAGACGG AGGTGTGTAA TGTCTATGCA 
GACGACTCCA TGCTGGCCTA CGACGACGAC GAAGATCAGC GAGTGCTGCG CTTGCAGTGG 
CCGAATCTGC AGGTGGATAA TCAGTTGTAC TCCAGCGGGA AATACGATTT CCCTGTTCTG 
CTCTGTGCTG AGCAATTGTA CGAGCGTCAC AATGGCCTGC CGCCGGTATA CGACCTGGAT 
GATATGTACA AGCAGCAGGC CCAGAGTGGC CCAGTCTCCC TGCTTACGTT TTACTTGTAC 
CAGGACGAAT GGCAGCTGCA GTCTGTGCGG TGTCATGTAC AGCCGTTTCG TGTCTACATT 
GAGGATGCCT ATCTGAATCA GTTGCTGGAG ACACTGGTGG AATGTGAGCC ATCCCATTGT 
GTGCACACGC CCCGCGAGGA GCAAGACAGG AGCTTGCTGC CCGATGGGCA AATCCTCTTG 
CCCGACCACG TCGTAGCGCA GTCATTGTAT ATAGCCGAGC CCTTTCGCCT GAACAGCTTC 
ACCGTGGAGC CCCTCAGCCT GCTCCTTTCT GTCCACACAT CCTCCAGACT TTACATAGCC 
CTGGACCACT CTCCACTCTC CTTCTCCCGC TACGAGCGCC AGCAGATCCT TACAGTTCCC 
CTACGCTTCG GACAGTCCTT GGGGCTGCAC TATCTCAGTG GAGCTATTTT CGGAGCCGGC 
TGGGTGGTGG GTTCCCTCGA GATTCTGGGA AGCCCCAGCG GGCTAGCTCG CTCCTTCAGC 
ACCGGTCTCC GAGACTTCAT CTCGATGCCT GTGGAGGGGC TGTTTCGAGG GCCCTGGGGC 
TTTATGGTGG GCGTGACACA GGGTTCTGCC TCTCTGCTGC GTAACGTAAC GGCGGGCACG 
GTCAATTCGG TTACTAAGCT GGCCGGTTCA GTGGCACGGA ATCTGGACAG GCTCACTCTG 
GACGCAGAAC ACATCGAACT GACGGAAGCC CGACGACGAG CCCGACCCCA GGGCTTTGCC 
GATGGCCTCA CGCAAGGCCT GACTGGCCTG GGCATCAGTC TGCTGGGTGC CGTGGGAGGA 
ATTGCACATC ACACCCTCGA AGCCCGGAGC TCTGTGGGTG TAATTGCGGG TCTTACCAAG 
GGCATTGTGG GAGCCTTCAC GAAACCCATC AGTGGAGCTG CCGAAATGCT GGCCCTCACT 
GGGCAAGGCG TTCTCCACAC GGTGGGCTTC AACGCCATGC CGCAACAGGT GGAGCCCAGC 
GTTTCTCGGA ACATAGCTCT GCACCCCGGC TCGTATCGAA TCTGGAGCAA TCTTCCAGAA 
GAGCTGCGCC GCGCTCAGAT ATTGTTCTTC TGCGAGATCA CGCTGCTCGC CAGGGATCAG 
CTGCGCCCGG CTCTGCTCTT CCTGACCACT TCGGTGCTCG CAATCATGGA GTCGGACAAC 
GATAATCTGA CCTTTGCCTC TGCAGTGAGC AAGGTGGCTG TTGCAGAGGA TCGCGACGAT 
CCCACAAAGT TTTACTTGAG CCTCAAGCCA GAGCAGATGG AGGACTTTGA AGGGCAAATA 
AACTACACCA ATGAGCGGAT CATGAAGTTC TTGAATACCT CTCGACTGCA CACGATAGCC 
AACGACTCGC TGAGCGATAT TTTCCAGCTA AATGAACACG AGATGGACTA CCACTTGAAA 
CGCCAAACGC AGTGTATATT CTACATCCAA CCCAACTACG GCAGGCATCT GATCCACTAC 
TTGAAGGTAG TCGGTCGGAT GCAGGCCTCA CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001357621.3
CDS	363..11558
Translation	MFKLESYITPILLSYVAKYVKNFRDEDAQVSLWEGEVTFQNLDLRLEVLEEELNLPVELVSGHIHELSIQVPWTKLMSEPVKIIINTIEFVAKLPDNESRQRRASYQKEQRRKAKKAAVEAEQQGAAPASSSVVVKKIINNITLQCHNIILKYVEDDIVVSMNVQCMNFSPAGENWKPTMVDVHPVSVSMRKLLQISDLTICLDKRNTAGRIEVCQEPVLYRCTLECRVLRKYNATTVSTSSTTRIGVFTKALDINVSSLQFPMVMRLVKMLLELKPAEMEEEALNPDDPEETAEGNEVPVGGTQPEGQSYLWWAWNLLPSFESQVPDPDPEETIGHAFDIGIYAEELNFQLKNSEFFTDQAMGGIKRIRYTPILRISLGGIYYERSQLKENDWTNVKAGLSSLCMEPLGAYRSEDPVDRVLVNTQESLKGRTFIDKSLFDEHFMFSDRTWCRHNYEDYVARNTDEYMLFRSPVLSFDIVEYRAPKPNSHPLAESQLKDLGLRVQYRLLSAGITFHFSQSFLQVKNVISDLLRPYDYSGYHSESVREEPQHEEHFNTDMTLADLEYLIGFAPTCNYKIELRNMTIQLYPRQQPKESSAANQHQLSTTVKQSLLPYLQLRLPLVEGTLCKPVNAHRLVQLITQLQDKPRELLNSCYNCYRFSVKNITLMILNTTPENGRAKLLNIPRMQLNVNRLLLPHLWRPNDAALETAEIQSEILTIEFSKRELTLAKRIIPLIASFNGTELCAVSHFVAQSTASSDVIKLQTLATKLRLNFHKYHTHLSANASLRSLNTDAYHTKMNMRNVVLSTTKNLNNKWLEVQLQLPLPLQETALQQQEQMPGTVLCLWVEHCRFTIDIYLLQFLKFPEHTTKRKSTENEIEMDVDAAASQSEHHFPQSVSNYSTISATALPLQPRRISRNNSRKISVPEETVHLSSERDERHTQMEPVTGLLMDSKPQKTALDLGQLAKRLGSMVVLAEIAQIRVDVCELMVRRRPAESDVEYTSIRLPRMKIKSSNCDFVSRGNIRSVILVDSEADLVNWVTDLNDLNVCYCRGDISEEVLAPVRTTITLAMSYKPIEEKLPGSLHSEKTAAVDQKQDQTSDKPVEQRGFGTYSINVHVDMSPINVFARRVKLLHEHFQIVSEIHRSLSPEEDGNVKWSSGPRLEVYTGSSQTYPVIKEFLELDPNFEPPQAKNVPNLDVSLIFFCQWTIPRISFEMESPTDRKQNKVVVTFEDLLLNVDKHSDFTKVTTKVENLGLNYYEAKPHGEWLQKDGFYVKMLGDSGNLPLISVVVTTVSLQDLYSKIGARNPRNRQHTISELLIELQPIEMVLDLDQITDFVIPLCGVIEIMRGNHQAPAIESRLTTVQDLPMVHLNSKGIYIYLPLATGRKCCSVLLLRIETVQLTPSLENPLVRQLVRQDIYQKAAELNMLSTPGALVEDRQYELSVRNVSLSTGNWLQTQKYHMEKSLASEHNNPAFEWNNQTRPTELDHMDIFRDFDFIMVYAPAIIYDRFFVCGQSVEYNCVTDFVATLTTHQINLISCILVRFQRLYSVLDQMYAKESPSLTELSSQSTHSITCTPVDISAKSSRLRHSGHSVRSSAKRSRSSFVDARSSSEVFVGSCQSDSGIHVGGGGVKGTQKSQEELQPSGVRYPQSVSFVAGIFVLKIYEVLELEELEQPVMKPLILLTVSQPSYMCTQSLKATITQASIFNLNVTVATPGTDPSVDPFNESVFDTLPGTLSSSGIPPPLLTVRSQCDRIHQTEVDVEVRKPIVLRLCETSIQQLVNNVIRIYNVLLETPCFAVRSERPVVQGTQLRQMKNHCYNADRLNLSFDQVTITLCDEERSYKCSSVWLDFSTSIKFNPRPQKAVIRSTIGSFYIQAGEKIFLHPLLMRLSVDLVSEPWCDDLLINATLKLHFLHVDASVWSILQLQKAHEGLGRILEYAHQEWQGFLEHRPAMGTPTEIAPGALIKCNPSVDSIGRCKRSLKTKAEFYQDDLRAGAFQFVYLNSESVLPMPYQVQIIKKNYGIICWRYPQPRQMTSIHIYPIPMPVSNPIHIKCSMEYFSETHETFLHYCDFQLSETATKQLSPQDRQICANIWRVVILQSLISVDGTCFEGDEDEDFHSIDSCRIEQAFKTDADNDFILHPKVLVGCMRIDTIFRAETVPRLQLLLCCQDMEVNLLNQPDGKGELPSLLKEYSPKRSIDSPQTFLTVHVGDLQVHSAVYARNDFSLETDFRARVKCLDYGFLNMLDIVEPMKFSSYAMFTHSPRSLKANFLLDKLRFNCGPYMIHTLLSSKHHWLEVLRQSEIRHTLMPKCVVVNRMQAGLSFGQTGTNEQISLAPHELQLYHFRSCAQGQELTFFIRNPETKQMEASSSVPIALKFEEEQRVRHLRVGKCCITIKVGKLSATQIYILVKGQIELISMVPFELITEFRQEGKRYDENTRPHEHLLLARGRASFYHTVVRNGDINLRLKLAAGLGKGRTGDIPLKTNNNLPWLVKVPMQNPVQQFVSVWVRILREDVDLDSAEPDFHPQKILICIWPIFEICNMLIDDLQATESTSGQSSKITAHGGRMSLQTATTHSTEHRVGFQFPAPLCSTSQEDYTLMLKTMDWHKFFYFDPAQWTIENSLHKLGKTVKPKWPLSDDEESRVLRRVSLTNAFDVEYRLKATREFSCTLGLEVSAWGMLINATGLDLSICQTRDRERHQLNANCLEMVPKLCGLFTIDVPFGCNWIASTPIYLEELTKTVENSPKRSMMLRCNSFVDIVVVRNEEVLRMMLEYRLEDGRRVFKLRSKFVITNFTDVSLNALPLTMDHKETSTREEVNAFSMANKSRSLVSVDGAKNCVGVTMDVFYDLNARAANHTCDTAFVYFLCFSVAGSRDISIPIPLAIPFTRRCFSLQSGRESVPFMITLIEKDNVHYLNIFRDTAPALIISNSTNVKFIVAQTSASGNSNVTCTNSEFVGRHFEWNQLVPPNSKCYYTPPQMYANFPDVEFTMCNLSLAVYNEKSCGKKKLGWSKPIRTDKSWKKFLHVPNHGEVKVVVCDKHRVIRVNIYYITQQLEFSVKDLRSRLTKPENRLLPSEQDSERQLDEIETVTATASATATDASDPLPDIVGCAHFSEECESPPQIKIRIFVKRFVFSLQTDSREKDYLKTEVCNVYADDSMLAYDDDEDQRVLRLQWPNLQVDNQLYSSGKYDFPVLLCAEQLYERHNGLPPVYDLDDMYKQQAQSGPVSLLTFYLYQDEWQLQSVRCHVQPFRVYIEDAYLNQLLETLVECEPSHCVHTPREEQDRSLLPDGQILLPDHVVAQSLYIAEPFRLNSFTVEPLSLLLSVHTSSRLYIALDHSPLSFSRYERQQILTVPLRFGQSLGLHYLSGAIFGAGWVVGSLEILGSPSGLARSFSTGLRDFISMPVEGLFRGPWGFMVGVTQGSASLLRNVTAGTVNSVTKLAGSVARNLDRLTLDAEHIELTEARRRARPQGFADGLTQGLTGLGISLLGAVGGIAHHTLEARSSVGVIAGLTKGIVGAFTKPISGAAEMLALTGQGVLHTVGFNAMPQQVEPSVSRNIALHPGSYRIWSNLPEELRRAQILFFCEITLLARDQLRPALLFLTTSVLAIMESDNDNLTFASAVSKVAVAEDRDDPTKFYLSLKPEQMEDFEGQINYTNERIMKFLNTSRLHTIANDSLSDIFQLNEHEMDYHLKRQTQCIFYIQPNYGRHLIHYLKVVGRMQASQ

Target ORF information:

RefSeq Version	XM_001357584.4
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA13781), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001357584.4

ORF Insert Sequence:

ATGTTCAAAC TAGAGTCCTA CATCACCCCG ATTCTGCTAA GCTACGTGGC CAAGTATGTG 
AAGAATTTCC GTGACGAAGA TGCCCAGGTG TCACTGTGGG AGGGTGAGGT GACGTTTCAG 
AACCTCGACC TGCGCCTGGA GGTGCTGGAG GAGGAGCTCA ATCTACCCGT GGAGCTCGTT 
TCGGGGCACA TCCACGAGCT GAGCATCCAG GTGCCATGGA CAAAGCTCAT GTCGGAGCCG 
GTGAAGATTA TCATAAACAC AATTGAGTTT GTGGCCAAGC TGCCGGACAA TGAGAGCAGG 
CAGCGTCGTG CCTCGTACCA AAAGGAGCAG CGCCGGAAGG CGAAAAAAGC CGCAGTGGAG 
GCTGAACAGC AGGGTGCCGC CCCCGCCAGT AGCTCAGTCG TGGTGAAAAA GATAATCAAC 
AACATCACCT TGCAGTGCCA CAACATCATT CTGAAGTACG TCGAAGACGA CATTGTCGTG 
TCCATGAATG TCCAGTGCAT GAACTTTAGC CCCGCCGGCG AGAACTGGAA GCCCACAATG 
GTGGACGTGC ACCCTGTGTC GGTTTCCATG CGCAAACTGC TGCAGATATC CGATCTTACC 
ATCTGCCTCG ATAAGCGGAA CACGGCCGGA AGGATTGAGG TGTGCCAGGA GCCGGTCCTC 
TATCGCTGCA CCCTGGAATG TCGCGTCCTG CGCAAGTACA ATGCCACCAC AGTCAGCACC 
TCGAGCACGA CCAGAATCGG GGTCTTCACC AAAGCGTTGG ACATTAACGT GTCCTCGCTG 
CAATTCCCGA TGGTCATGAG GTTGGTCAAA ATGCTGCTGG AACTCAAGCC TGCGGAGATG 
GAGGAGGAAG CTCTCAACCC AGACGATCCA GAGGAAACAG CGGAGGGCAA CGAAGTGCCG 
GTGGGGGGTA CACAGCCCGA GGGTCAGAGT TATCTGTGGT GGGCCTGGAA CCTTTTGCCC 
AGCTTCGAGT CGCAGGTCCC CGACCCCGAC CCAGAGGAGA CCATTGGCCA TGCCTTCGAT 
ATAGGCATAT ATGCTGAGGA GCTGAACTTT CAGCTGAAGA ATTCGGAGTT CTTCACCGAT 
CAGGCCATGG GAGGGATAAA GCGGATCCGC TATACACCCA TCCTGCGCAT CAGTTTGGGC 
GGCATCTACT ACGAGAGATC GCAGTTGAAG GAGAACGACT GGACGAACGT CAAGGCGGGG 
CTCTCGAGCC TTTGCATGGA GCCCTTGGGG GCATACAGGA GCGAGGATCC AGTCGATCGG 
GTGTTGGTCA ATACCCAGGA GTCTTTAAAG GGGCGCACAT TCATCGACAA GAGCCTGTTC 
GACGAGCACT TTATGTTCTC GGATCGCACC TGGTGCCGCC ACAACTACGA GGACTACGTG 
GCCAGGAACA CAGATGAGTA TATGCTCTTC CGCAGCCCCG TGCTGTCGTT CGATATTGTT 
GAGTACCGGG CCCCAAAGCC GAACAGCCAT CCCCTGGCCG AGAGCCAGCT GAAGGACCTG 
GGACTGCGCG TTCAGTATCG TCTACTATCC GCTGGGATTA CTTTCCACTT CTCACAGTCA 
TTTCTCCAGG TTAAGAATGT AATTAGCGAT TTATTGCGTC CTTACGATTA CTCGGGATAT 
CATTCAGAGT CGGTCAGGGA GGAGCCCCAG CACGAGGAGC ACTTTAACAC AGACATGACC 
CTGGCGGACT TGGAGTACCT CATTGGATTC GCTCCCACCT GCAACTACAA GATCGAGCTG 
CGGAACATGA CCATTCAGCT GTATCCGCGC CAGCAGCCAA AAGAGTCCTC GGCAGCCAAT 
CAGCATCAGC TGTCGACCAC GGTCAAGCAA TCCCTGCTGC CATATCTCCA GCTAAGACTA 
CCCCTAGTCG AGGGCACGCT GTGCAAGCCA GTGAACGCAC ACCGTCTGGT CCAGTTGATC 
ACCCAGCTGC AGGACAAGCC GCGAGAGCTC CTCAATTCGT GCTACAATTG CTACAGATTC 
AGTGTGAAGA ATATCACGCT GATGATCCTA AATACCACGC CCGAGAACGG CCGCGCAAAG 
CTGCTGAACA TTCCCCGGAT GCAGTTGAAC GTTAATCGTC TCCTGCTCCC CCATCTCTGG 
CGACCCAACG ATGCTGCGCT GGAAACGGCG GAGATTCAGT CGGAGATCCT GACTATAGAG 
TTCTCGAAGC GCGAGCTGAC CCTCGCCAAG CGCATCATTC CGCTGATAGC GAGCTTCAAC 
GGCACAGAGC TCTGTGCTGT GTCCCATTTT GTGGCGCAAT CCACTGCCAG TTCGGATGTG 
ATCAAGCTGC AAACGCTGGC CACAAAACTG CGACTGAATT TCCACAAGTA TCACACGCAT 
CTATCGGCAA ATGCGTCGCT GCGAAGCCTC AACACAGATG CCTACCACAC GAAGATGAAC 
ATGCGCAACG TGGTGCTGTC CACCACCAAG AACCTGAACA ACAAGTGGCT AGAGGTGCAG 
CTGCAGCTGC CACTGCCACT GCAGGAGACA GCCTTGCAGC AACAGGAGCA GATGCCCGGG 
ACGGTTTTGT GTCTGTGGGT GGAGCATTGT CGCTTTACCA TAGATATATA TCTCCTGCAG 
TTCCTCAAGT TCCCAGAGCA CACAACGAAA AGGAAATCAA CGGAAAATGA GATCGAAATG 
GATGTAGATG CTGCCGCAAG CCAGTCCGAG CATCATTTTC CTCAATCGGT GTCAAACTAT 
TCCACGATAT CTGCCACAGC GTTGCCCCTT CAACCGCGTC GAATCAGCCG CAACAATAGC 
CGAAAGATCT CCGTCCCTGA GGAGACAGTG CATCTCAGCT CCGAGCGGGA TGAGAGGCAC 
ACACAGATGG AACCAGTAAC CGGTTTATTG ATGGACAGCA AGCCGCAGAA GACTGCTCTA 
GATCTTGGTC AGCTGGCGAA GCGTCTCGGC TCCATGGTAG TGCTCGCAGA GATTGCCCAG 
ATCCGGGTAG ACGTGTGCGA GCTCATGGTG CGCAGGAGGC CCGCAGAGTC GGATGTGGAA 
TACACGTCCA TACGGCTGCC CCGCATGAAA ATCAAGTCCA GCAACTGCGA TTTCGTTTCC 
CGGGGGAACA TCAGGAGTGT CATTTTGGTG GATTCCGAAG CAGATCTGGT CAACTGGGTC 
ACGGATCTGA ACGACTTGAA TGTCTGCTAC TGCAGGGGAG ACATATCAGA AGAGGTTTTG 
GCACCAGTCA GGACTACCAT CACTCTGGCC ATGTCCTATA AGCCAATCGA GGAGAAGCTT 
CCGGGATCCC TTCATTCCGA GAAGACGGCC GCGGTGGATC AGAAGCAGGA CCAGACCTCC 
GATAAACCTG TAGAACAGCG AGGCTTTGGT ACTTACTCAA TAAATGTGCA CGTGGACATG 
TCGCCCATTA ATGTTTTTGC TCGAAGGGTG AAACTTCTAC ACGAGCATTT TCAAATCGTT 
TCCGAGATTC ACAGATCTCT CAGTCCTGAA GAGGATGGCA ATGTCAAATG GAGCTCGGGT 
CCCCGACTGG AGGTTTATAC GGGCAGCTCC CAAACCTATC CCGTGATAAA GGAGTTTCTG 
GAATTGGATC CCAACTTTGA GCCCCCTCAA GCGAAAAATG TTCCCAATTT AGATGTCTCG 
CTGATATTCT TCTGCCAGTG GACCATACCA CGGATCAGCT TCGAAATGGA GAGCCCCACC 
GATCGCAAAC AGAACAAGGT TGTCGTGACT TTCGAAGATC TACTACTGAA CGTGGACAAG 
CATTCGGACT TTACGAAGGT CACCACCAAA GTGGAGAACC TGGGACTCAA CTACTACGAG 
GCCAAGCCGC ACGGGGAGTG GCTCCAGAAG GATGGCTTCT ACGTCAAAAT GCTGGGCGAC 
AGCGGCAATT TGCCGCTGAT TAGTGTGGTG GTTACCACCG TCAGCCTGCA GGATCTGTAC 
TCGAAGATTG GAGCCAGAAA TCCCCGCAAT AGGCAGCACA CAATTTCTGA GCTGCTGATC 
GAACTGCAGC CCATTGAAAT GGTCCTCGAC CTGGATCAAA TCACAGACTT TGTGATTCCC 
CTGTGCGGAG TGATAGAAAT AATGAGGGGC AATCATCAGG CACCAGCTAT TGAGAGCCGC 
TTAACCACCG TTCAGGACTT GCCCATGGTG CATCTGAACA GCAAAGGAAT CTATATCTAC 
CTGCCATTGG CCACGGGCAG AAAGTGCTGC AGTGTCTTGT TGCTAAGGAT TGAGACTGTG 
CAGCTAACGC CTAGCCTGGA GAATCCTTTA GTGCGGCAAC TGGTTCGCCA GGACATCTAC 
CAGAAGGCCG CCGAGCTGAA CATGCTGAGC ACCCCCGGAG CCCTGGTCGA GGATCGTCAG 
TACGAGCTGA GCGTCAGAAA TGTGTCCCTG TCTACTGGGA ACTGGCTGCA GACCCAAAAG 
TATCACATGG AAAAGAGTCT GGCCAGTGAG CACAACAATC CGGCCTTCGA GTGGAACAAC 
CAGACACGTC CCACTGAGCT GGACCACATG GATATATTCA GGGACTTCGA CTTCATCATG 
GTCTATGCCC CGGCCATTAT CTACGATCGG TTTTTCGTCT GCGGCCAGAG TGTGGAGTAC 
AACTGTGTGA CGGACTTTGT GGCCACTCTG ACCACACACC AAATCAATCT GATATCCTGC 
ATCCTAGTGC GTTTTCAGCG TTTGTACAGT GTCCTCGATC AGATGTATGC CAAAGAGTCG 
CCTTCGCTGA CGGAGCTGTC ATCACAGTCA ACGCACAGCA TCACGTGCAC CCCCGTGGAC 
ATTTCGGCCA AGAGCAGTCG GCTAAGACAC TCTGGCCATT CGGTCCGGTC CAGCGCCAAG 
CGAAGTCGTT CCAGTTTCGT GGATGCCAGG AGCAGCAGCG AAGTCTTTGT GGGCTCCTGC 
CAAAGTGATT CCGGAATACA CGTGGGAGGG GGTGGTGTAA AGGGAACTCA GAAATCGCAG 
GAGGAGCTGC AACCGAGTGG CGTACGCTAC CCGCAAAGTG TCTCCTTTGT GGCTGGGATA 
TTTGTGCTAA AGATCTACGA AGTCCTGGAG CTGGAGGAAC TGGAGCAGCC AGTGATGAAG 
CCGCTCATTC TGCTGACAGT CTCGCAGCCC AGCTATATGT GCACCCAAAG TCTGAAGGCA 
ACCATCACGC AGGCATCCAT TTTCAATCTA AACGTAACTG TGGCCACTCC TGGTACGGAC 
CCTTCGGTGG ACCCCTTCAA TGAGTCTGTG TTTGACACCC TGCCAGGTAC TCTCAGCAGT 
TCGGGGATTC CCCCACCGCT GCTCACTGTG CGGTCCCAGT GCGATCGCAT CCATCAAACC 
GAAGTGGATG TGGAGGTGCG CAAGCCCATA GTGCTGCGCC TGTGCGAGAC GAGCATCCAG 
CAACTGGTCA ACAATGTCAT CCGCATCTAC AACGTGCTCC TCGAGACTCC CTGCTTCGCG 
GTGCGCAGCG AGCGGCCTGT GGTCCAGGGC ACACAGTTGC GGCAGATGAA GAACCACTGC 
TACAATGCGG ACCGATTGAA TCTGTCCTTT GATCAGGTCA CCATCACGCT CTGCGACGAG 
GAACGGTCCT ACAAATGCAG CTCCGTTTGG CTGGACTTTA GCACCAGCAT CAAGTTCAAT 
CCGCGTCCCC AGAAGGCCGT CATTAGATCG ACGATTGGCT CCTTCTACAT CCAGGCTGGC 
GAGAAGATCT TCCTGCATCC CCTGTTGATG CGTTTGTCCG TGGATCTGGT CAGCGAGCCA 
TGGTGTGACG ATCTCCTGAT CAACGCTACT CTCAAGCTAC ACTTTCTGCA CGTCGATGCG 
AGTGTTTGGA GTATTCTGCA ACTGCAAAAG GCCCATGAAG GTCTCGGCCG GATCCTTGAG 
TATGCCCATC AGGAGTGGCA GGGCTTTCTG GAACATCGTC CGGCAATGGG CACTCCAACA 
GAGATTGCAC CCGGAGCGCT GATCAAGTGC AATCCATCGG TCGACTCGAT CGGGCGCTGC 
AAACGCTCGC TGAAAACTAA AGCAGAATTC TATCAGGACG ACCTCAGGGC GGGCGCCTTC 
CAATTTGTCT ACTTGAACTC CGAGTCCGTG CTGCCTATGC CATACCAGGT GCAGATCATC 
AAGAAGAACT ACGGGATTAT CTGCTGGCGC TATCCACAGC CACGCCAGAT GACCAGCATC 
CACATCTATC CGATTCCCAT GCCAGTCTCA AACCCCATAC ACATCAAGTG CAGCATGGAG 
TACTTCAGTG AAACGCACGA GACTTTTCTG CACTACTGCG ACTTCCAGCT GTCGGAGACG 
GCCACCAAGC AGCTATCCCC ACAAGATCGT CAGATCTGTG CGAACATCTG GAGAGTGGTC 
ATTCTGCAGT CGCTGATTTC TGTCGATGGC ACCTGCTTTG AAGGAGATGA GGATGAAGAT 
TTCCATTCTA TCGACAGCTG TCGCATTGAG CAGGCCTTTA AGACCGATGC AGACAACGAT 
TTCATATTGC ATCCAAAGGT TCTTGTGGGC TGCATGCGCA TCGATACCAT CTTCAGGGCA 
GAGACAGTGC CCAGGCTGCA GCTGCTGCTC TGTTGCCAGG ACATGGAAGT AAACCTTCTG 
AATCAGCCGG ATGGCAAGGG GGAACTGCCT TCGCTTTTGA AAGAATACTC CCCCAAGCGG 
TCAATAGACT CCCCACAGAC CTTTCTCACA GTGCACGTGG GAGATCTGCA GGTACACTCG 
GCTGTCTATG CCAGGAATGA CTTCAGTCTG GAGACGGACT TCCGCGCGCG CGTGAAGTGC 
TTGGACTATG GCTTCCTCAA CATGCTGGAC ATCGTGGAGC CTATGAAATT CTCCAGCTAC 
GCGATGTTCA CCCACTCTCC GCGCTCGCTG AAAGCGAACT TTCTGCTGGA CAAGCTCCGC 
TTCAACTGCG GTCCCTACAT GATCCACACT CTGCTCAGCT CCAAGCATCA CTGGCTGGAA 
GTGCTGCGCC AGAGCGAGAT CCGTCACACT TTAATGCCCA AGTGCGTGGT GGTCAACCGC 
ATGCAGGCGG GCCTAAGCTT CGGGCAGACG GGCACGAACG AGCAAATATC CCTGGCACCC 
CATGAGCTGC AGCTCTACCA CTTCCGCAGC TGTGCGCAAG GGCAAGAGCT GACGTTCTTC 
ATTCGGAATC CAGAGACCAA GCAGATGGAG GCCAGCAGCT CGGTGCCCAT AGCCCTGAAA 
TTCGAGGAGG AGCAGAGAGT GCGTCATCTG CGGGTGGGAA AGTGTTGCAT CACAATCAAG 
GTGGGAAAAC TGTCCGCCAC GCAGATCTAT ATACTGGTGA AGGGCCAGAT TGAGCTGATC 
AGCATGGTGC CCTTTGAATT GATCACCGAA TTCCGCCAAG AGGGAAAACG CTACGACGAG 
AATACGAGAC CACACGAGCA CCTGCTTTTG GCCAGGGGAA GGGCATCCTT CTATCATACG 
GTGGTGCGTA ATGGAGACAT TAACTTGCGT CTTAAGTTAG CGGCTGGTCT CGGCAAGGGA 
CGCACTGGAG ATATTCCTTT GAAAACTAAC AATAATCTGC CTTGGCTGGT CAAGGTGCCC 
ATGCAAAACC CCGTCCAGCA GTTTGTCAGC GTGTGGGTGC GCATCCTGCG CGAGGATGTG 
GATTTGGACT CAGCCGAACC GGACTTTCAT CCGCAAAAGA TATTGATTTG CATTTGGCCC 
ATCTTCGAGA TATGCAACAT GCTGATCGAC GATCTTCAGG CCACAGAAAG TACGAGTGGG 
CAGAGCTCCA AGATCACAGC CCATGGCGGA AGGATGTCTC TGCAGACGGC TACCACGCAC 
AGCACAGAGC ATCGCGTTGG CTTTCAGTTT CCGGCTCCCT TGTGCAGCAC CTCCCAGGAG 
GATTATACAC TCATGCTCAA GACCATGGAC TGGCACAAGT TCTTTTACTT CGATCCCGCT 
CAGTGGACGA TTGAGAACTC GCTGCACAAA CTGGGGAAGA CGGTGAAGCC CAAGTGGCCG 
CTGAGCGACG ATGAGGAATC GCGCGTGCTT CGCCGTGTGT CACTGACCAA CGCATTCGAT 
GTGGAATACC GCTTAAAGGC CACGCGGGAG TTTTCCTGCA CTTTGGGATT GGAGGTTTCT 
GCCTGGGGAA TGCTCATCAA TGCCACCGGT CTGGACCTGA GCATCTGCCA GACCAGGGAC 
CGCGAGCGCC ATCAACTGAA CGCCAACTGT CTGGAAATGG TGCCCAAATT GTGCGGTCTG 
TTCACAATTG ATGTGCCTTT TGGCTGCAAT TGGATTGCCT CGACGCCCAT TTACCTGGAG 
GAACTGACCA AGACAGTGGA GAACAGCCCC AAGCGGAGCA TGATGCTCCG CTGTAATTCC 
TTTGTGGACA TCGTGGTGGT GCGAAACGAG GAGGTGCTGC GAATGATGCT GGAGTATAGG 
CTGGAGGATG GGCGCCGTGT ATTTAAGCTG CGCTCCAAAT TCGTAATCAC CAATTTCACA 
GATGTCTCTC TGAATGCCCT GCCCTTGACC ATGGATCACA AGGAGACCTC CACAAGGGAG 
GAAGTGAATG CGTTCAGCAT GGCCAACAAG AGTCGCAGTC TCGTATCTGT GGATGGGGCA 
AAGAATTGCG TTGGAGTGAC GATGGACGTT TTCTACGATC TTAATGCCCG TGCGGCTAAT 
CATACCTGTG ATACGGCTTT CGTCTACTTT CTGTGCTTCA GTGTGGCTGG TAGTCGCGAC 
ATATCTATAC CCATTCCCCT GGCCATACCC TTCACGAGAC GCTGCTTCAG CCTGCAGTCT 
GGACGGGAAT CGGTGCCATT CATGATCACT CTCATTGAGA AGGACAATGT CCATTATCTG 
AATATATTCC GCGATACGGC CCCGGCACTG ATCATCAGCA ACAGCACGAA TGTGAAGTTT 
ATTGTGGCCC AGACGAGTGC CTCCGGCAAC AGCAATGTGA CTTGCACAAA CTCCGAGTTC 
GTAGGGAGGC ATTTTGAATG GAATCAGTTG GTCCCGCCGA ACAGTAAATG TTACTACACC 
CCGCCGCAGA TGTACGCCAA CTTTCCCGAT GTGGAGTTTA CCATGTGTAA TCTGTCGTTA 
GCTGTATATA ATGAAAAGTC CTGTGGCAAG AAGAAACTCG GCTGGTCAAA GCCCATAAGG 
ACCGACAAGT CGTGGAAGAA GTTCTTGCAT GTGCCGAATC ATGGCGAAGT GAAGGTGGTC 
GTCTGTGATA AGCATCGCGT CATACGCGTC AACATCTACT ACATAACCCA GCAACTGGAG 
TTTTCTGTGA AGGATCTACG CTCACGGTTG ACAAAGCCAG AGAATCGTTT GCTGCCCTCA 
GAGCAGGATT CGGAAAGACA GTTGGACGAG ATCGAGACTG TTACTGCTAC GGCGTCAGCT 
ACGGCTACTG ATGCCAGCGA TCCATTGCCG GACATTGTGG GCTGTGCCCA TTTTAGCGAG 
GAATGCGAGT CCCCGCCACA GATCAAGATA CGAATATTCG TGAAGCGCTT TGTGTTCAGC 
CTCCAGACGG ACAGTCGGGA GAAGGACTAC TTAAAGACGG AGGTGTGTAA TGTCTATGCA 
GACGACTCCA TGCTGGCCTA CGACGACGAC GAAGATCAGC GAGTGCTGCG CTTGCAGTGG 
CCGAATCTGC AGGTGGATAA TCAGTTGTAC TCCAGCGGGA AATACGATTT CCCTGTTCTG 
CTCTGTGCTG AGCAATTGTA CGAGCGTCAC AATGGCCTGC CGCCGGTATA CGACCTGGAT 
GATATGTACA AGCAGCAGGC CCAGAGTGGC CCAGTCTCCC TGCTTACGTT TTACTTGTAC 
CAGGACGAAT GGCAGCTGCA GTCTGTGCGG TGTCATGTAC AGCCGTTTCG TGTCTACATT 
GAGGATGCCT ATCTGAATCA GTTGCTGGAG ACACTGGTGG AATGTGAGCC ATCCCATTGT 
GTGCACACGC CCCGCGAGGA GCAAGACAGG AGCTTGCTGC CCGATGGGCA AATCCTCTTG 
CCCGACCACG TCGTAGCGCA GTCATTGTAT ATAGCCGAGC CCTTTCGCCT GAACAGCTTC 
ACCGTGGAGC CCCTCAGCCT GCTCCTTTCT GTCCACACAT CCTCCAGACT TTACATAGCC 
CTGGACCACT CTCCACTCTC CTTCTCCCGC TACGAGCGCC AGCAGATCCT TACAGTTCCC 
CTACGCTTCG GACAGTCCTT GGGGCTGCAC TATCTCAGTG GAGCTATTTT CGGAGCCGGC 
TGGGTGGTGG GTTCCCTCGA GATTCTGGGA AGCCCCAGCG GGCTAGCTCG CTCCTTCAGC 
ACCGGTCTCC GAGACTTCAT CTCGATGCCT GTGGAGGGGC TGTTTCGAGG GCCCTGGGGC 
TTTATGGTGG GCGTGACACA GGGTTCTGCC TCTCTGCTGC GTAACGTAAC GGCGGGCACG 
GTCAATTCGG TTACTAAGCT GGCCGGTTCA GTGGCACGGA ATCTGGACAG GCTCACTCTG 
GACGCAGAAC ACATCGAACT GACGGAAGCC CGACGACGAG CCCGACCCCA GGGCTTTGCC 
GATGGCCTCA CGCAAGGCCT GACTGGCCTG GGCATCAGTC TGCTGGGTGC CGTGGGAGGA 
ATTGCACATC ACACCCTCGA AGCCCGGAGC TCTGTGGGTG TAATTGCGGG TCTTACCAAG 
GGCATTGTGG GAGCCTTCAC GAAACCCATC AGTGGAGCTG CCGAAATGCT GGCCCTCACT 
GGGCAAGGCG TTCTCCACAC GGTGGGCTTC AACGCCATGC CGCAACAGGT GGAGCCCAGC 
GTTTCTCGGA ACATAGCTCT GCACCCCGGC TCGTATCGAA TCTGGAGCAA TCTTCCAGAA 
GAGCTGCGCC GCGCTCAGAT ATTGTTCTTC TGCGAGATCA CGCTGCTCGC CAGGGATCAG 
CTGCGCCCGG CTCTGCTCTT CCTGACCACT TCGGTGCTCG CAATCATGGA GTCGGACAAC 
GATAATCTGA CCTTTGCCTC TGCAGTGAGC AAGGTGGCTG TTGCAGAGGA TCGCGACGAT 
CCCACAAAGT TTTACTTGAG CCTCAAGCCA GAGCAGATGG AGGACTTTGA AGGGCAAATA 
AACTACACCA ATGAGCGGAT CATGAAGTTC TTGAATACCT CTCGACTGCA CACGATAGCC 
AACGACTCGC TGAGCGATAT TTTCCAGCTA AATGAACACG AGATGGACTA CCACTTGAAA 
CGCCAAACGC AGTGTATATT CTACATCCAA CCCAACTACG GCAGGCATCT GATCCACTAC 
TTGAAGGTAG TCGGTCGGAT GCAGGCCTCA CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA13781 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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