LOC8078313 cDNA ORF clone, Sorghum bicolor(sorghum)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8078313
Entrez Gene ID	8078313
Full Name	phosphoenolpyruvate carboxylase 1
Synonyms	CP21,PEPC
Gene Type	protein-coding
Organism	Sorghum bicolor(sorghum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002451810.1	XP_002451855.1	hypothetical protein
XM_002451810.2	XP_002451855.1	phosphoenolpyruvate carboxylase 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Sorghum bicolor genome and the diversification of grasses.
Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang H, Wang X, Wicker T, Bharti AK, Chapman J, Feltus FA, Gowik U, Grigoriev IV, Lyons E, Maher CA, Martis M, Narechania A, Otillar RP, Penning BW, Salamov AA, Wang Y, Zhang L, Carpita NC, Freeling M, Gingle AR, Hash CT, Keller B, Klein P, Kresovich S, McCann MC, Ming R, Peterson DG, Mehboob-ur-Rahman, Ware D, Westhoff P, Mayer KF, Messing J, Rokhsar DS
Nature457(7229)551-6(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC8078313 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8078313 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSc16361	XM_002451810.2 Latest version!	Sorghum bicolor phosphoenolpyruvate carboxylase 1 (LOC8078313), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OSc16361	XM_002451810.1	Sorghum bicolor hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8078313 ( XM_002451810.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_002451855.1 Sorghum bicolor hypothetical protein, mRNA.

Price & Availability↑

CloneID	OSc16361
Clone ID Related Accession (Same CDS sequence)	XM_002451810.1 , XM_002451810.2
Accession Version	XM_002451810.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1247328000000
Organism	Sorghum bicolor(Sorghum)
Product	hypothetical protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012873).

ATGCCGGAGC GGCACCAGTC GATCGACGCG CAGCTGCGGC TGCTGGCCCC CGGGAAGGTC 
TCCGAGGACG ACAAGCTCGT CGAGTACGAC GCCCTCCTCG TCGACCGCTT CCTTGACATC 
CTCCAGGACC TGCACGGCCC CCACCTGCGC GAATTCGTCC AGGAGTGCTA CGAGCTCTCG 
GCGGAGTACG AGAACGACCG GGATGAGGCT CGGCTCGGCG AGCTCGGGAG CAAGCTCACC 
AGCCTGCCGC CGGGGGACTC CATCGTCGTC GCCAGTTCCT TCTCGCACAT GCTCAACCTC 
GCCAACCTCG CCGAGGAAGT GCAGGTCGCG CAGCGCCGCC GGATCAAGCT CAAGCGTGGG 
GACTTCGCCG ACGAGGCCTC CGCGCCGACG GAGTCGGACA TCGAGGAGAC GCTCAAGCGC 
CTCGTCTCGC AGCTCGGCAA GTCGCGGGAG GAGGTCTTCG ACGCGCTCAA GAACCAGACC 
GTCGACCTCG TCTTCACCGC CCACCCCACG CAGTCCGTCA GGAGGTCTCT TCTCCAGAAG 
CACGGCAGGA TCCGTAACTG CCTGAGGCAG CTCTATGCCA AGGACATCAC TGCTGACGAC 
AAACAGGAGC TTGACGAGGC ACTTCAGAGG GAGATTCAAG CTGCTTTCAG AACTGATGAA 
ATCCGCAGAA CCCCTCCCAC TCCTCAAGAT GAAATGCGTG CTGGAATGAG TTACTTCCAT 
GAAACTATAT GGAAGGGTGT ACCAAAATTC TTGCGCCGTA TTGACACTGC TCTGAAAAAT 
ATTGGGATCA ATGAACGTCT CCCTTACAAT GCTCCTCTCA TTCAGTTCTC TTCCTGGATG 
GGTGGTGATC GTGATGGAAA TCCTAGAGTA ACACCAGAGG TTACACGGGA TGTATGCTTG 
TTGGCAAGAA TGATGGCTGC TAACCTGTAC TTCTCTCAGA TCGAAGATCT AATGTTTGAG 
CTCTCTATGT GGCGCTGCAG TGATGAACTT CGGATCCGTG CAGATGAACT ACATCGCTCT 
TCAAAAAGAG CTGCAAAGCA CTATATAGAA TTCTGGAAGC AAGTTCCTCC AAATGAACCA 
TATCGTGTCA TACTTGGTGA TGTCAGGGAT AAATTGTACT ACACACGTGA ACGTTCTCGT 
CACCTATTGT CATCTGGAAT TTCTGAGATT CCAGAGGAGG CAACTTTTAC TAATGTTGAA 
CAGTTTCTGG AACCTCTTGA GCTCTGCTAT AGATCATTAT GTGCTTGTGG TGACAAACCT 
ATAGCTGACG GAAGTCTTCT TGATTTCTTG CGTCAAGTAT CAACTTTTGG GCTTGCCCTT 
GTGAAGCTTG ACATCAGGCA GGAATCTGAT CGACACACCG ATGTTCTTGA TTCAATAACG 
ACACATCTTG GAATTGGCTC CTATGCTGAA TGGTCCGAGG AGAAACGCCA GGATTGGCTG 
TTGTCTGAAC TGAGGGGCAA ACGTCCATTG TTTGGTTCTG ATCTTCCTCA GACTGAAGAG 
ACTGCTGATG TTCTAGGCAC GTTTCATGTC CTTGCAGAGC TTCCAGCAGA TTGCTTTGGC 
GCGTATATCA TCTCAATGGC AACTGCCCCA TCTGATGTTC TCGCGGTCGA GCTTTTGCAG 
CGGGAGTGCC ATGTAAAGCA GCCACTGAGA GTTGTTCCAC TCTTTGAGAA ACTAGCAGAT 
CTTGAAGCAG CTCCAGCGGC TGTAGCACGA CTCTTTTCAA TTGACTGGTA CATGAATAGG 
ATTAATGGCA AGCAGGAGGT CATGATTGGA TATTCAGACT CTGGCAAAGA TGCCGGCCGT 
CTCTCTGCAG CATGGCAAAT GTATAAAGCA CAAGAAGAGC TCATCAAGGT GGCAAAGCAT 
TATGGAGTGA AGTTGACAAT GTTTCATGGA AGAGGTGGAA CAGTTGGCAG AGGAGGTGGT 
CCAACTCATC TGGCCATATT ATCTCAGCCA CCAGACACGA TACATGGGTC GCTTCGTGTA 
ACAGTTCAAG GCGAAGTTAT TGAGCACTCC TTTGGAGAGG AGCTCTTGTG CTTTAGAACT 
TTGCAACGCT ACACTGCAGC TACTCTTGAA CATGGCATGC ATCCTCCAAT TTCCCCCAAG 
CCAGAATGGC GTGCTCTGAT GGATGAAATG GCTGTTGTAG CAACTAAAGA ATATCGATCA 
ATTGTCTTCC AAGAACCACG CTTCGTTGAA TACTTCAGAT CGGCTACGCC TGAGACTGAA 
TATGGTAGGA TGAATATTGG CAGCCGTCCG TCAAAGAGGA AGCCTAGCGG GGGAATAGAA 
TCGCTCCGTG CAATTCCATG GATCTTTGCT TGGACACAAA CAAGGTTCCA TCTGCCTGTT 
TGGCTTGGAT TTGGTGCAGC GATCAAGCAC ATCATGCAGA AGGACATCAG GAACATCCAT 
GTACTGAAAG AAATGTACAA TGAGTGGCCA TTCTTCAGGG TCACCCTTGA TTTGCTTGAG 
ATGGTTTTCG CCAAGGGAGA CCCGGGAATT GCAGCTGTAT ATGACAAATT GCTAGTGGCT 
GAGGATTTGC AATCCTTCGG TGAGCAACTA AGGAAGAACT ATGAGGAGAC AAAAGAGCTC 
CTCCTTCAGG TTGCTGGTCA CAAGGACGTC CTTGAAGGCG ATCCTTACCT GAAGCAGCGT 
CTGCGGCTGC GTGAGTCCTA CATCACCACT CTGAACGTGT GCCAGGCCTA CACCCTGAAG 
CGGATACGTG ACCCGAGCTT CCAGGTGAGC CCGCAGCCGC CCCTGTCCAA GGAGTTCACC 
GACGAGAGCC AGCCGGTGGA GCTGGTGCAG CTGAACCAGC AGAGCGAGTA CGCGCCAGGC 
CTGGAGGACA CACTGATCCT GACCATGAAG GGCATCGCTG CCGGCATGCA GAATACCGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002451855.1
CDS	206..3088
Translation	MPERHQSIDAQLRLLAPGKVSEDDKLVEYDALLVDRFLDILQDLHGPHLREFVQECYELSAEYENDRDEARLGELGSKLTSLPPGDSIVVASSFSHMLNLANLAEEVQVAQRRRIKLKRGDFADEASAPTESDIEETLKRLVSQLGKSREEVFDALKNQTVDLVFTAHPTQSVRRSLLQKHGRIRNCLRQLYAKDITADDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSYFHETIWKGVPKFLRRIDTALKNIGINERLPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLLARMMAANLYFSQIEDLMFELSMWRCSDELRIRADELHRSSKRAAKHYIEFWKQVPPNEPYRVILGDVRDKLYYTRERSRHLLSSGISEIPEEATFTNVEQFLEPLELCYRSLCACGDKPIADGSLLDFLRQVSTFGLALVKLDIRQESDRHTDVLDSITTHLGIGSYAEWSEEKRQDWLLSELRGKRPLFGSDLPQTEETADVLGTFHVLAELPADCFGAYIISMATAPSDVLAVELLQRECHVKQPLRVVPLFEKLADLEAAPAAVARLFSIDWYMNRINGKQEVMIGYSDSGKDAGRLSAAWQMYKAQEELIKVAKHYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGSLRVTVQGEVIEHSFGEELLCFRTLQRYTAATLEHGMHPPISPKPEWRALMDEMAVVATKEYRSIVFQEPRFVEYFRSATPETEYGRMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGAAIKHIMQKDIRNIHVLKEMYNEWPFFRVTLDLLEMVFAKGDPGIAAVYDKLLVAEDLQSFGEQLRKNYEETKELLLQVAGHKDVLEGDPYLKQRLRLRESYITTLNVCQAYTLKRIRDPSFQVSPQPPLSKEFTDESQPVELVQLNQQSEYAPGLEDTLILTMKGIAAGMQNTG

Target ORF information:

RefSeq Version	XM_002451810.1
Organism	Sorghum bicolor(Sorghum)
Definition	Sorghum bicolor hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002451810.1

ORF Insert Sequence:

ATGCCGGAGC GGCACCAGTC GATCGACGCG CAGCTGCGGC TGCTGGCCCC CGGGAAGGTC 
TCCGAGGACG ACAAGCTCGT CGAGTACGAC GCCCTCCTCG TCGACCGCTT CCTTGACATC 
CTCCAGGACC TGCACGGCCC CCACCTGCGC GAATTCGTCC AGGAGTGCTA CGAGCTCTCG 
GCGGAGTACG AGAACGACCG GGATGAGGCT CGGCTCGGCG AGCTCGGGAG CAAGCTCACC 
AGCCTGCCGC CGGGGGACTC CATCGTCGTC GCCAGTTCCT TCTCGCACAT GCTCAACCTC 
GCCAACCTCG CCGAGGAAGT GCAGGTCGCG CAGCGCCGCC GGATCAAGCT CAAGCGTGGG 
GACTTCGCCG ACGAGGCCTC CGCGCCGACG GAGTCGGACA TCGAGGAGAC GCTCAAGCGC 
CTCGTCTCGC AGCTCGGCAA GTCGCGGGAG GAGGTCTTCG ACGCGCTCAA GAACCAGACC 
GTCGACCTCG TCTTCACCGC CCACCCCACG CAGTCCGTCA GGAGGTCTCT TCTCCAGAAG 
CACGGCAGGA TCCGTAACTG CCTGAGGCAG CTCTATGCCA AGGACATCAC TGCTGACGAC 
AAACAGGAGC TTGACGAGGC ACTTCAGAGG GAGATTCAAG CTGCTTTCAG AACTGATGAA 
ATCCGCAGAA CCCCTCCCAC TCCTCAAGAT GAAATGCGTG CTGGAATGAG TTACTTCCAT 
GAAACTATAT GGAAGGGTGT ACCAAAATTC TTGCGCCGTA TTGACACTGC TCTGAAAAAT 
ATTGGGATCA ATGAACGTCT CCCTTACAAT GCTCCTCTCA TTCAGTTCTC TTCCTGGATG 
GGTGGTGATC GTGATGGAAA TCCTAGAGTA ACACCAGAGG TTACACGGGA TGTATGCTTG 
TTGGCAAGAA TGATGGCTGC TAACCTGTAC TTCTCTCAGA TCGAAGATCT AATGTTTGAG 
CTCTCTATGT GGCGCTGCAG TGATGAACTT CGGATCCGTG CAGATGAACT ACATCGCTCT 
TCAAAAAGAG CTGCAAAGCA CTATATAGAA TTCTGGAAGC AAGTTCCTCC AAATGAACCA 
TATCGTGTCA TACTTGGTGA TGTCAGGGAT AAATTGTACT ACACACGTGA ACGTTCTCGT 
CACCTATTGT CATCTGGAAT TTCTGAGATT CCAGAGGAGG CAACTTTTAC TAATGTTGAA 
CAGTTTCTGG AACCTCTTGA GCTCTGCTAT AGATCATTAT GTGCTTGTGG TGACAAACCT 
ATAGCTGACG GAAGTCTTCT TGATTTCTTG CGTCAAGTAT CAACTTTTGG GCTTGCCCTT 
GTGAAGCTTG ACATCAGGCA GGAATCTGAT CGACACACCG ATGTTCTTGA TTCAATAACG 
ACACATCTTG GAATTGGCTC CTATGCTGAA TGGTCCGAGG AGAAACGCCA GGATTGGCTG 
TTGTCTGAAC TGAGGGGCAA ACGTCCATTG TTTGGTTCTG ATCTTCCTCA GACTGAAGAG 
ACTGCTGATG TTCTAGGCAC GTTTCATGTC CTTGCAGAGC TTCCAGCAGA TTGCTTTGGC 
GCGTATATCA TCTCAATGGC AACTGCCCCA TCTGATGTTC TCGCGGTCGA GCTTTTGCAG 
CGGGAGTGCC ATGTAAAGCA GCCACTGAGA GTTGTTCCAC TCTTTGAGAA ACTAGCAGAT 
CTTGAAGCAG CTCCAGCGGC TGTAGCACGA CTCTTTTCAA TTGACTGGTA CATGAATAGG 
ATTAATGGCA AGCAGGAGGT CATGATTGGA TATTCAGACT CTGGCAAAGA TGCCGGCCGT 
CTCTCTGCAG CATGGCAAAT GTATAAAGCA CAAGAAGAGC TCATCAAGGT GGCAAAGCAT 
TATGGAGTGA AGTTGACAAT GTTTCATGGA AGAGGTGGAA CAGTTGGCAG AGGAGGTGGT 
CCAACTCATC TGGCCATATT ATCTCAGCCA CCAGACACGA TACATGGGTC GCTTCGTGTA 
ACAGTTCAAG GCGAAGTTAT TGAGCACTCC TTTGGAGAGG AGCTCTTGTG CTTTAGAACT 
TTGCAACGCT ACACTGCAGC TACTCTTGAA CATGGCATGC ATCCTCCAAT TTCCCCCAAG 
CCAGAATGGC GTGCTCTGAT GGATGAAATG GCTGTTGTAG CAACTAAAGA ATATCGATCA 
ATTGTCTTCC AAGAACCACG CTTCGTTGAA TACTTCAGAT CGGCTACGCC TGAGACTGAA 
TATGGTAGGA TGAATATTGG CAGCCGTCCG TCAAAGAGGA AGCCTAGCGG GGGAATAGAA 
TCGCTCCGTG CAATTCCATG GATCTTTGCT TGGACACAAA CAAGGTTCCA TCTGCCTGTT 
TGGCTTGGAT TTGGTGCAGC GATCAAGCAC ATCATGCAGA AGGACATCAG GAACATCCAT 
GTACTGAAAG AAATGTACAA TGAGTGGCCA TTCTTCAGGG TCACCCTTGA TTTGCTTGAG 
ATGGTTTTCG CCAAGGGAGA CCCGGGAATT GCAGCTGTAT ATGACAAATT GCTAGTGGCT 
GAGGATTTGC AATCCTTCGG TGAGCAACTA AGGAAGAACT ATGAGGAGAC AAAAGAGCTC 
CTCCTTCAGG TTGCTGGTCA CAAGGACGTC CTTGAAGGCG ATCCTTACCT GAAGCAGCGT 
CTGCGGCTGC GTGAGTCCTA CATCACCACT CTGAACGTGT GCCAGGCCTA CACCCTGAAG 
CGGATACGTG ACCCGAGCTT CCAGGTGAGC CCGCAGCCGC CCCTGTCCAA GGAGTTCACC 
GACGAGAGCC AGCCGGTGGA GCTGGTGCAG CTGAACCAGC AGAGCGAGTA CGCGCCAGGC 
CTGGAGGACA CACTGATCCT GACCATGAAG GGCATCGCTG CCGGCATGCA GAATACCGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8078313 ( XM_002451810.2 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_002451855.1 Sorghum bicolor phosphoenolpyruvate carboxylase 1 (LOC8078313), mRNA.

Price & Availability↑

CloneID	OSc16361
Clone ID Related Accession (Same CDS sequence)	XM_002451810.1 , XM_002451810.2
Accession Version	XM_002451810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	phosphoenolpyruvate carboxylase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012873.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 13, 2017 this sequence version replaced XM_002451810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGAGC GGCACCAGTC GATCGACGCG CAGCTGCGGC TGCTGGCCCC CGGGAAGGTC 
TCCGAGGACG ACAAGCTCGT CGAGTACGAC GCCCTCCTCG TCGACCGCTT CCTTGACATC 
CTCCAGGACC TGCACGGCCC CCACCTGCGC GAATTCGTCC AGGAGTGCTA CGAGCTCTCG 
GCGGAGTACG AGAACGACCG GGATGAGGCT CGGCTCGGCG AGCTCGGGAG CAAGCTCACC 
AGCCTGCCGC CGGGGGACTC CATCGTCGTC GCCAGTTCCT TCTCGCACAT GCTCAACCTC 
GCCAACCTCG CCGAGGAAGT GCAGGTCGCG CAGCGCCGCC GGATCAAGCT CAAGCGTGGG 
GACTTCGCCG ACGAGGCCTC CGCGCCGACG GAGTCGGACA TCGAGGAGAC GCTCAAGCGC 
CTCGTCTCGC AGCTCGGCAA GTCGCGGGAG GAGGTCTTCG ACGCGCTCAA GAACCAGACC 
GTCGACCTCG TCTTCACCGC CCACCCCACG CAGTCCGTCA GGAGGTCTCT TCTCCAGAAG 
CACGGCAGGA TCCGTAACTG CCTGAGGCAG CTCTATGCCA AGGACATCAC TGCTGACGAC 
AAACAGGAGC TTGACGAGGC ACTTCAGAGG GAGATTCAAG CTGCTTTCAG AACTGATGAA 
ATCCGCAGAA CCCCTCCCAC TCCTCAAGAT GAAATGCGTG CTGGAATGAG TTACTTCCAT 
GAAACTATAT GGAAGGGTGT ACCAAAATTC TTGCGCCGTA TTGACACTGC TCTGAAAAAT 
ATTGGGATCA ATGAACGTCT CCCTTACAAT GCTCCTCTCA TTCAGTTCTC TTCCTGGATG 
GGTGGTGATC GTGATGGAAA TCCTAGAGTA ACACCAGAGG TTACACGGGA TGTATGCTTG 
TTGGCAAGAA TGATGGCTGC TAACCTGTAC TTCTCTCAGA TCGAAGATCT AATGTTTGAG 
CTCTCTATGT GGCGCTGCAG TGATGAACTT CGGATCCGTG CAGATGAACT ACATCGCTCT 
TCAAAAAGAG CTGCAAAGCA CTATATAGAA TTCTGGAAGC AAGTTCCTCC AAATGAACCA 
TATCGTGTCA TACTTGGTGA TGTCAGGGAT AAATTGTACT ACACACGTGA ACGTTCTCGT 
CACCTATTGT CATCTGGAAT TTCTGAGATT CCAGAGGAGG CAACTTTTAC TAATGTTGAA 
CAGTTTCTGG AACCTCTTGA GCTCTGCTAT AGATCATTAT GTGCTTGTGG TGACAAACCT 
ATAGCTGACG GAAGTCTTCT TGATTTCTTG CGTCAAGTAT CAACTTTTGG GCTTGCCCTT 
GTGAAGCTTG ACATCAGGCA GGAATCTGAT CGACACACCG ATGTTCTTGA TTCAATAACG 
ACACATCTTG GAATTGGCTC CTATGCTGAA TGGTCCGAGG AGAAACGCCA GGATTGGCTG 
TTGTCTGAAC TGAGGGGCAA ACGTCCATTG TTTGGTTCTG ATCTTCCTCA GACTGAAGAG 
ACTGCTGATG TTCTAGGCAC GTTTCATGTC CTTGCAGAGC TTCCAGCAGA TTGCTTTGGC 
GCGTATATCA TCTCAATGGC AACTGCCCCA TCTGATGTTC TCGCGGTCGA GCTTTTGCAG 
CGGGAGTGCC ATGTAAAGCA GCCACTGAGA GTTGTTCCAC TCTTTGAGAA ACTAGCAGAT 
CTTGAAGCAG CTCCAGCGGC TGTAGCACGA CTCTTTTCAA TTGACTGGTA CATGAATAGG 
ATTAATGGCA AGCAGGAGGT CATGATTGGA TATTCAGACT CTGGCAAAGA TGCCGGCCGT 
CTCTCTGCAG CATGGCAAAT GTATAAAGCA CAAGAAGAGC TCATCAAGGT GGCAAAGCAT 
TATGGAGTGA AGTTGACAAT GTTTCATGGA AGAGGTGGAA CAGTTGGCAG AGGAGGTGGT 
CCAACTCATC TGGCCATATT ATCTCAGCCA CCAGACACGA TACATGGGTC GCTTCGTGTA 
ACAGTTCAAG GCGAAGTTAT TGAGCACTCC TTTGGAGAGG AGCTCTTGTG CTTTAGAACT 
TTGCAACGCT ACACTGCAGC TACTCTTGAA CATGGCATGC ATCCTCCAAT TTCCCCCAAG 
CCAGAATGGC GTGCTCTGAT GGATGAAATG GCTGTTGTAG CAACTAAAGA ATATCGATCA 
ATTGTCTTCC AAGAACCACG CTTCGTTGAA TACTTCAGAT CGGCTACGCC TGAGACTGAA 
TATGGTAGGA TGAATATTGG CAGCCGTCCG TCAAAGAGGA AGCCTAGCGG GGGAATAGAA 
TCGCTCCGTG CAATTCCATG GATCTTTGCT TGGACACAAA CAAGGTTCCA TCTGCCTGTT 
TGGCTTGGAT TTGGTGCAGC GATCAAGCAC ATCATGCAGA AGGACATCAG GAACATCCAT 
GTACTGAAAG AAATGTACAA TGAGTGGCCA TTCTTCAGGG TCACCCTTGA TTTGCTTGAG 
ATGGTTTTCG CCAAGGGAGA CCCGGGAATT GCAGCTGTAT ATGACAAATT GCTAGTGGCT 
GAGGATTTGC AATCCTTCGG TGAGCAACTA AGGAAGAACT ATGAGGAGAC AAAAGAGCTC 
CTCCTTCAGG TTGCTGGTCA CAAGGACGTC CTTGAAGGCG ATCCTTACCT GAAGCAGCGT 
CTGCGGCTGC GTGAGTCCTA CATCACCACT CTGAACGTGT GCCAGGCCTA CACCCTGAAG 
CGGATACGTG ACCCGAGCTT CCAGGTGAGC CCGCAGCCGC CCCTGTCCAA GGAGTTCACC 
GACGAGAGCC AGCCGGTGGA GCTGGTGCAG CTGAACCAGC AGAGCGAGTA CGCGCCAGGC 
CTGGAGGACA CACTGATCCT GACCATGAAG GGCATCGCTG CCGGCATGCA GAATACCGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002451855.1
CDS	205..3087
Translation	MPERHQSIDAQLRLLAPGKVSEDDKLVEYDALLVDRFLDILQDLHGPHLREFVQECYELSAEYENDRDEARLGELGSKLTSLPPGDSIVVASSFSHMLNLANLAEEVQVAQRRRIKLKRGDFADEASAPTESDIEETLKRLVSQLGKSREEVFDALKNQTVDLVFTAHPTQSVRRSLLQKHGRIRNCLRQLYAKDITADDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSYFHETIWKGVPKFLRRIDTALKNIGINERLPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLLARMMAANLYFSQIEDLMFELSMWRCSDELRIRADELHRSSKRAAKHYIEFWKQVPPNEPYRVILGDVRDKLYYTRERSRHLLSSGISEIPEEATFTNVEQFLEPLELCYRSLCACGDKPIADGSLLDFLRQVSTFGLALVKLDIRQESDRHTDVLDSITTHLGIGSYAEWSEEKRQDWLLSELRGKRPLFGSDLPQTEETADVLGTFHVLAELPADCFGAYIISMATAPSDVLAVELLQRECHVKQPLRVVPLFEKLADLEAAPAAVARLFSIDWYMNRINGKQEVMIGYSDSGKDAGRLSAAWQMYKAQEELIKVAKHYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGSLRVTVQGEVIEHSFGEELLCFRTLQRYTAATLEHGMHPPISPKPEWRALMDEMAVVATKEYRSIVFQEPRFVEYFRSATPETEYGRMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGAAIKHIMQKDIRNIHVLKEMYNEWPFFRVTLDLLEMVFAKGDPGIAAVYDKLLVAEDLQSFGEQLRKNYEETKELLLQVAGHKDVLEGDPYLKQRLRLRESYITTLNVCQAYTLKRIRDPSFQVSPQPPLSKEFTDESQPVELVQLNQQSEYAPGLEDTLILTMKGIAAGMQNTG

Target ORF information:

RefSeq Version	XM_002451810.2
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor phosphoenolpyruvate carboxylase 1 (LOC8078313), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002451810.2

ORF Insert Sequence:

ATGCCGGAGC GGCACCAGTC GATCGACGCG CAGCTGCGGC TGCTGGCCCC CGGGAAGGTC 
TCCGAGGACG ACAAGCTCGT CGAGTACGAC GCCCTCCTCG TCGACCGCTT CCTTGACATC 
CTCCAGGACC TGCACGGCCC CCACCTGCGC GAATTCGTCC AGGAGTGCTA CGAGCTCTCG 
GCGGAGTACG AGAACGACCG GGATGAGGCT CGGCTCGGCG AGCTCGGGAG CAAGCTCACC 
AGCCTGCCGC CGGGGGACTC CATCGTCGTC GCCAGTTCCT TCTCGCACAT GCTCAACCTC 
GCCAACCTCG CCGAGGAAGT GCAGGTCGCG CAGCGCCGCC GGATCAAGCT CAAGCGTGGG 
GACTTCGCCG ACGAGGCCTC CGCGCCGACG GAGTCGGACA TCGAGGAGAC GCTCAAGCGC 
CTCGTCTCGC AGCTCGGCAA GTCGCGGGAG GAGGTCTTCG ACGCGCTCAA GAACCAGACC 
GTCGACCTCG TCTTCACCGC CCACCCCACG CAGTCCGTCA GGAGGTCTCT TCTCCAGAAG 
CACGGCAGGA TCCGTAACTG CCTGAGGCAG CTCTATGCCA AGGACATCAC TGCTGACGAC 
AAACAGGAGC TTGACGAGGC ACTTCAGAGG GAGATTCAAG CTGCTTTCAG AACTGATGAA 
ATCCGCAGAA CCCCTCCCAC TCCTCAAGAT GAAATGCGTG CTGGAATGAG TTACTTCCAT 
GAAACTATAT GGAAGGGTGT ACCAAAATTC TTGCGCCGTA TTGACACTGC TCTGAAAAAT 
ATTGGGATCA ATGAACGTCT CCCTTACAAT GCTCCTCTCA TTCAGTTCTC TTCCTGGATG 
GGTGGTGATC GTGATGGAAA TCCTAGAGTA ACACCAGAGG TTACACGGGA TGTATGCTTG 
TTGGCAAGAA TGATGGCTGC TAACCTGTAC TTCTCTCAGA TCGAAGATCT AATGTTTGAG 
CTCTCTATGT GGCGCTGCAG TGATGAACTT CGGATCCGTG CAGATGAACT ACATCGCTCT 
TCAAAAAGAG CTGCAAAGCA CTATATAGAA TTCTGGAAGC AAGTTCCTCC AAATGAACCA 
TATCGTGTCA TACTTGGTGA TGTCAGGGAT AAATTGTACT ACACACGTGA ACGTTCTCGT 
CACCTATTGT CATCTGGAAT TTCTGAGATT CCAGAGGAGG CAACTTTTAC TAATGTTGAA 
CAGTTTCTGG AACCTCTTGA GCTCTGCTAT AGATCATTAT GTGCTTGTGG TGACAAACCT 
ATAGCTGACG GAAGTCTTCT TGATTTCTTG CGTCAAGTAT CAACTTTTGG GCTTGCCCTT 
GTGAAGCTTG ACATCAGGCA GGAATCTGAT CGACACACCG ATGTTCTTGA TTCAATAACG 
ACACATCTTG GAATTGGCTC CTATGCTGAA TGGTCCGAGG AGAAACGCCA GGATTGGCTG 
TTGTCTGAAC TGAGGGGCAA ACGTCCATTG TTTGGTTCTG ATCTTCCTCA GACTGAAGAG 
ACTGCTGATG TTCTAGGCAC GTTTCATGTC CTTGCAGAGC TTCCAGCAGA TTGCTTTGGC 
GCGTATATCA TCTCAATGGC AACTGCCCCA TCTGATGTTC TCGCGGTCGA GCTTTTGCAG 
CGGGAGTGCC ATGTAAAGCA GCCACTGAGA GTTGTTCCAC TCTTTGAGAA ACTAGCAGAT 
CTTGAAGCAG CTCCAGCGGC TGTAGCACGA CTCTTTTCAA TTGACTGGTA CATGAATAGG 
ATTAATGGCA AGCAGGAGGT CATGATTGGA TATTCAGACT CTGGCAAAGA TGCCGGCCGT 
CTCTCTGCAG CATGGCAAAT GTATAAAGCA CAAGAAGAGC TCATCAAGGT GGCAAAGCAT 
TATGGAGTGA AGTTGACAAT GTTTCATGGA AGAGGTGGAA CAGTTGGCAG AGGAGGTGGT 
CCAACTCATC TGGCCATATT ATCTCAGCCA CCAGACACGA TACATGGGTC GCTTCGTGTA 
ACAGTTCAAG GCGAAGTTAT TGAGCACTCC TTTGGAGAGG AGCTCTTGTG CTTTAGAACT 
TTGCAACGCT ACACTGCAGC TACTCTTGAA CATGGCATGC ATCCTCCAAT TTCCCCCAAG 
CCAGAATGGC GTGCTCTGAT GGATGAAATG GCTGTTGTAG CAACTAAAGA ATATCGATCA 
ATTGTCTTCC AAGAACCACG CTTCGTTGAA TACTTCAGAT CGGCTACGCC TGAGACTGAA 
TATGGTAGGA TGAATATTGG CAGCCGTCCG TCAAAGAGGA AGCCTAGCGG GGGAATAGAA 
TCGCTCCGTG CAATTCCATG GATCTTTGCT TGGACACAAA CAAGGTTCCA TCTGCCTGTT 
TGGCTTGGAT TTGGTGCAGC GATCAAGCAC ATCATGCAGA AGGACATCAG GAACATCCAT 
GTACTGAAAG AAATGTACAA TGAGTGGCCA TTCTTCAGGG TCACCCTTGA TTTGCTTGAG 
ATGGTTTTCG CCAAGGGAGA CCCGGGAATT GCAGCTGTAT ATGACAAATT GCTAGTGGCT 
GAGGATTTGC AATCCTTCGG TGAGCAACTA AGGAAGAACT ATGAGGAGAC AAAAGAGCTC 
CTCCTTCAGG TTGCTGGTCA CAAGGACGTC CTTGAAGGCG ATCCTTACCT GAAGCAGCGT 
CTGCGGCTGC GTGAGTCCTA CATCACCACT CTGAACGTGT GCCAGGCCTA CACCCTGAAG 
CGGATACGTG ACCCGAGCTT CCAGGTGAGC CCGCAGCCGC CCCTGTCCAA GGAGTTCACC 
GACGAGAGCC AGCCGGTGGA GCTGGTGCAG CTGAACCAGC AGAGCGAGTA CGCGCCAGGC 
CTGGAGGACA CACTGATCCT GACCATGAAG GGCATCGCTG CCGGCATGCA GAATACCGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Sorghum bicolor genome and the diversification of grasses.
Nature457(7229)551-6(2009 Jan)
Paterson AH,Bowers JE,Bruggmann R,Dubchak I,Grimwood J,Gundlach H,Haberer G,Hellsten U,Mitros T,Poliakov A,Schmutz J,Spannagl M,Tang H,Wang X,Wicker T,Bharti AK,Chapman J,Feltus FA,Gowik U,Grigoriev IV,Lyons E,Maher CA,Martis M,Narechania A,Otillar RP,Penning BW,Salamov AA,Wang Y,Zhang L,Carpita NC,Freeling M,Gingle AR,Hash CT,Keller B,Klein P,Kresovich S,McCann MC,Ming R,Peterson DG,Mehboob-ur-Rahman,Ware D,Westhoff P,Mayer KF,Messing J,Rokhsar DS

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LOC8078313 cDNA ORF clone, Sorghum bicolor(sorghum)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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