LINJ_11_0250 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_11_0250
Entrez Gene ID	5067203
Full Name	putative protein kinase
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001463791.2	XP_001463828.2	putative protein kinase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_11_0250 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_11_0250 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc01105	XM_001463791.2 Latest version!	Leishmania infantum JPCM5 putative protein kinase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_11_0250 ( XM_001463791.2 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001463828.2 Leishmania infantum JPCM5 putative protein kinase partial mRNA.

Price & Availability↑

CloneID	OLc01105
Clone ID Related Accession (Same CDS sequence)	XM_001463791.2
Accession Version	XM_001463791.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009395). On Jul 14, 2011 this sequence version replaced XM_001463791.1. COMPLETENESS: incomplete on both ends.

ATGAGCACCA GCGAGGCAGA AGGCAGCATC AGCGGGAGCG GCACCCTGAG AGGCCGTGCC 
TGTGCGAGTG GTGGGGCAGG CAAGTTCGAC AAGGCAATGA AGATGTACCA AAAGTCTATG 
GCGATTAGGC GGCGCCTGAA GAGCATGGCA AGTAGCACCA CGCGCTCACT TGGCGACAGA 
GATGATGACG GTTTCGGCGG CGGCGCACTC AATGAAGAGA GCGCCGCCGC TCAGACCTCA 
TTGAGCCCGC TGCGCGGGCC GATGCTCCGT TCATCGACCC AAATCACGCG CGTGGCGCCC 
GCGTCATCTA TCGCGTCCTT GGCCTCTCCG CAGCATTCAC GGGTGCTGCC GGAAATGGCC 
TTCGGCGTCG CGACCGCGTC TGAGCGGACA GCGATGGCTT CGCAACCTGC CATCGCACTT 
GATGCCGGGT CGTCCGTTGA GACGCGCTCG TCAATGGCAG CGTTGATGTT GTACTCGCAC 
GTGCCGCTGC CTGCTCTCAA CTCATTCAAG CGCCTCTCCG ACCCGCCTGA CGTCGAGCGG 
GAAACGGCGC CATCTTCCTC ATCTCCATTC TCTCTGGCCA TCCCATCGCG AGGCCGCACG 
AAAGGCGGCA GTGGCAATCG CCACAAAAAG GTGGACGCCA GCGGCGGCGA GAACGACGCA 
GTGTGGCGGA AGAGAAGCCC CACCTGCAGC GCCCACCTAG ACTTCCCCTA TCGCTTCTCT 
GCCGAGCGAC AACGCACTGC GTCGCGGCAC AACAGCGGCG GAACGAGCGA GGGCTTTGGC 
GTCGGCATCC ACAAGTATGC TGGCCAGCCC TCAACCTCGA CAGTAGACTC GCCGCTGCTG 
TCCCCGATGC CGTCCTTTCA CCAGATGATG GGCCGCTCCT ACGATGTGCC CGCACTGAAC 
GCGATTTTCC GTGGCTTCAG CAACGGTGTC GATCGGTGGC AGCAGGACAG CAGATTGCTG 
CGGTCGCCGC CTTCACCAAC TCATGCCACC ATGCCAGCCG CGCCGCCCAC GTCGGTGCCA 
TCAGTGTCGA CAAGCCTACA GCTTTCATCC TCACTCGTCT CAAAGATGTC TGTGCCGCCC 
CCTGCAGCTG CGGTGACAAG GCAGATACCC ACGGTATCGG TGTCATCATC GGCAGAGACT 
ACAGCAGCAG CAGATGTGCA TGCAATGCCG GCAGGCAACG ACGCCTTTTC GTACCTGGGG 
CCACGCCTTT CGGCGCAGGC GCCGTCAAGG AAAGATCGAA GCAACTCGTA CCCGGCCGAT 
AAGGCGCGCC CAGAAGCGAG CGGACGCAGC ACGGTCGAAG GCCGTGATGG TGTGCACGCG 
AGCGGATCTC TCCCGCTGGT CAAGACGATG GCGTCGGCTC CGAACCTCAA GCCAAACGAA 
GCGAGCGTTC TACCGCTGGG GCCGCCGCCG CTGGACGAGA GCTCGGCAGC GACGACAACA 
ACGGCGCAGT TTTACTCCAC CGCCTTGTTG GCGAAGACGT TCGACGGTGC TCAGTACCTC 
AATGACTACA TTCTACTGAA CGAAATCGGC AGTGGATCCA CAGGGCGCGT CGTTCTGGCG 
TTCAGCACGA GCATGAACAA GAGTGTTGCC ATCAAGATCA TTCTGAAGCC GAAAGAGAAG 
TACCGGTTGC AGCACCGCGT GTCGGCTTCC CCGTCCACGT CCGTGCTCGG GCAGCACAGC 
AGCGGGGGAA CTTTTAGTGG GGGAAGAGCA GCGACTGCGC TGGCTACAAC GACACCCGAA 
GCCATGCAGA ACTTCAAGTC ATCGTCCTCT TCGGCTCGGA GGCGGAGCAA GCACCGCCAG 
ACACCGTCGA CCCCGTTCAC GACGGCGGCG GACAAGACGC GTAACCTGCA GCGCGAAGTC 
GAGGTTATGA AGGACCTCAA TCACCCCAAT ATTGTTCGTC TTTACGAGGT CATCAACGAC 
CCCAAGGCGA ACTCCCTCTT CCTGGTCCTA CAGTACGTGG ATAACGGCGC TGTCGCGCAG 
CTCGACTCGA CTGGACATAT TCGAGCTCCG TTGCACCCGT GGACCGTTCT GCCGATCGCA 
GCGCAAATCA GTGACGGTCT CGTGTACCTT CATGAGCAGC ATATTGTGCA CCGTGATATC 
AAGCCCGAAA ACATCCTCGT TAACCGCGAC GGGCACGCCT TTCTCGCCGA CTTCGGTGTC 
GCAGAGCTCA TGAGCGCGAA GGCAGGCCAA CCAACTGCCG CCACGCTCAC CTATCAAGGC 
ACACCACTGT TCATGGCTCC TGAAATCTAC GCCGGCGTCG ACGACGAAGA CAGAGACGAG 
GATGCGGAGC AAGCCCCAGG TCGGTGCACA CGGCGGAGAA GCAGCAATGA TGCTGACGAG 
AGCAGCAACA TGACGGCGAG CCCCTTGTGC GAGGAGCGGC GCGACTCACG CGTGATCGAC 
CCGTTTGCCC TCGACGTCTG GGCGCTAGGG GTCACCTTCT ACACACTGCT CATCGGCCAC 
GTGCCCTTCA CATCCATGCT CCAGATCTCG CAGACGGTTG AGAAGGGTGT GAACATACCA 
GCCTCGCTCC CAGAGCAGTG GCGCACCGTG CTGCGGCGCA TGATGGAGCC GCGTCAAGAG 
CTGCGGATCT CCTCAGCTGA GCTGTGCCAC ATGCTGCACG CGATGCTAGC GGAGCAAGAG 
GCCGCAGAGG TGAGGGCTGG CGGACGCAGC CGGAAGGCAT CGCTCAGCAC ACGCTCACGA 
AGCGCAACCC TCGGCAATCC TCGCAAAGCC TCTCACCAGT GTTGCGCCGA GGGTCGAATG 
GCGCGCAGTG CAAGTCTTGC TGCTGACGCG GAAGAGGAGA GTATGAACCT TGGCAGCAGC 
AGCAGCAGCG ATGAGAGCGG CACAAGCGGG TACAGCAAGG GCATCCTCGG CCTTAGCATC 
ACATCCGTCG GCCTCAACAT CCTGCGACCG ACACGAATCC GAAAAAAACA GGAGCTGAGT 
TGCAGGGAGA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001463828.2
CDS	1..2955
Translation	MSTSEAEGSISGSGTLRGRACASGGAGKFDKAMKMYQKSMAIRRRLKSMASSTTRSLGDRDDDGFGGGALNEESAAAQTSLSPLRGPMLRSSTQITRVAPASSIASLASPQHSRVLPEMAFGVATASERTAMASQPAIALDAGSSVETRSSMAALMLYSHVPLPALNSFKRLSDPPDVERETAPSSSSPFSLAIPSRGRTKGGSGNRHKKVDASGGENDAVWRKRSPTCSAHLDFPYRFSAERQRTASRHNSGGTSEGFGVGIHKYAGQPSTSTVDSPLLSPMPSFHQMMGRSYDVPALNAIFRGFSNGVDRWQQDSRLLRSPPSPTHATMPAAPPTSVPSVSTSLQLSSSLVSKMSVPPPAAAVTRQIPTVSVSSSAETTAAADVHAMPAGNDAFSYLGPRLSAQAPSRKDRSNSYPADKARPEASGRSTVEGRDGVHASGSLPLVKTMASAPNLKPNEASVLPLGPPPLDESSAATTTTAQFYSTALLAKTFDGAQYLNDYILLNEIGSGSTGRVVLAFSTSMNKSVAIKIILKPKEKYRLQHRVSASPSTSVLGQHSSGGTFSGGRAATALATTTPEAMQNFKSSSSSARRRSKHRQTPSTPFTTAADKTRNLQREVEVMKDLNHPNIVRLYEVINDPKANSLFLVLQYVDNGAVAQLDSTGHIRAPLHPWTVLPIAAQISDGLVYLHEQHIVHRDIKPENILVNRDGHAFLADFGVAELMSAKAGQPTAATLTYQGTPLFMAPEIYAGVDDEDRDEDAEQAPGRCTRRRSSNDADESSNMTASPLCEERRDSRVIDPFALDVWALGVTFYTLLIGHVPFTSMLQISQTVEKGVNIPASLPEQWRTVLRRMMEPRQELRISSAELCHMLHAMLAEQEAAEVRAGGRSRKASLSTRSRSATLGNPRKASHQCCAEGRMARSASLAADAEEESMNLGSSSSSDESGTSGYSKGILGLSITSVGLNILRPTRIRKKQELSCRES

Target ORF information:

RefSeq Version	XM_001463791.2
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative protein kinase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001463791.2

ORF Insert Sequence:

ATGAGCACCA GCGAGGCAGA AGGCAGCATC AGCGGGAGCG GCACCCTGAG AGGCCGTGCC 
TGTGCGAGTG GTGGGGCAGG CAAGTTCGAC AAGGCAATGA AGATGTACCA AAAGTCTATG 
GCGATTAGGC GGCGCCTGAA GAGCATGGCA AGTAGCACCA CGCGCTCACT TGGCGACAGA 
GATGATGACG GTTTCGGCGG CGGCGCACTC AATGAAGAGA GCGCCGCCGC TCAGACCTCA 
TTGAGCCCGC TGCGCGGGCC GATGCTCCGT TCATCGACCC AAATCACGCG CGTGGCGCCC 
GCGTCATCTA TCGCGTCCTT GGCCTCTCCG CAGCATTCAC GGGTGCTGCC GGAAATGGCC 
TTCGGCGTCG CGACCGCGTC TGAGCGGACA GCGATGGCTT CGCAACCTGC CATCGCACTT 
GATGCCGGGT CGTCCGTTGA GACGCGCTCG TCAATGGCAG CGTTGATGTT GTACTCGCAC 
GTGCCGCTGC CTGCTCTCAA CTCATTCAAG CGCCTCTCCG ACCCGCCTGA CGTCGAGCGG 
GAAACGGCGC CATCTTCCTC ATCTCCATTC TCTCTGGCCA TCCCATCGCG AGGCCGCACG 
AAAGGCGGCA GTGGCAATCG CCACAAAAAG GTGGACGCCA GCGGCGGCGA GAACGACGCA 
GTGTGGCGGA AGAGAAGCCC CACCTGCAGC GCCCACCTAG ACTTCCCCTA TCGCTTCTCT 
GCCGAGCGAC AACGCACTGC GTCGCGGCAC AACAGCGGCG GAACGAGCGA GGGCTTTGGC 
GTCGGCATCC ACAAGTATGC TGGCCAGCCC TCAACCTCGA CAGTAGACTC GCCGCTGCTG 
TCCCCGATGC CGTCCTTTCA CCAGATGATG GGCCGCTCCT ACGATGTGCC CGCACTGAAC 
GCGATTTTCC GTGGCTTCAG CAACGGTGTC GATCGGTGGC AGCAGGACAG CAGATTGCTG 
CGGTCGCCGC CTTCACCAAC TCATGCCACC ATGCCAGCCG CGCCGCCCAC GTCGGTGCCA 
TCAGTGTCGA CAAGCCTACA GCTTTCATCC TCACTCGTCT CAAAGATGTC TGTGCCGCCC 
CCTGCAGCTG CGGTGACAAG GCAGATACCC ACGGTATCGG TGTCATCATC GGCAGAGACT 
ACAGCAGCAG CAGATGTGCA TGCAATGCCG GCAGGCAACG ACGCCTTTTC GTACCTGGGG 
CCACGCCTTT CGGCGCAGGC GCCGTCAAGG AAAGATCGAA GCAACTCGTA CCCGGCCGAT 
AAGGCGCGCC CAGAAGCGAG CGGACGCAGC ACGGTCGAAG GCCGTGATGG TGTGCACGCG 
AGCGGATCTC TCCCGCTGGT CAAGACGATG GCGTCGGCTC CGAACCTCAA GCCAAACGAA 
GCGAGCGTTC TACCGCTGGG GCCGCCGCCG CTGGACGAGA GCTCGGCAGC GACGACAACA 
ACGGCGCAGT TTTACTCCAC CGCCTTGTTG GCGAAGACGT TCGACGGTGC TCAGTACCTC 
AATGACTACA TTCTACTGAA CGAAATCGGC AGTGGATCCA CAGGGCGCGT CGTTCTGGCG 
TTCAGCACGA GCATGAACAA GAGTGTTGCC ATCAAGATCA TTCTGAAGCC GAAAGAGAAG 
TACCGGTTGC AGCACCGCGT GTCGGCTTCC CCGTCCACGT CCGTGCTCGG GCAGCACAGC 
AGCGGGGGAA CTTTTAGTGG GGGAAGAGCA GCGACTGCGC TGGCTACAAC GACACCCGAA 
GCCATGCAGA ACTTCAAGTC ATCGTCCTCT TCGGCTCGGA GGCGGAGCAA GCACCGCCAG 
ACACCGTCGA CCCCGTTCAC GACGGCGGCG GACAAGACGC GTAACCTGCA GCGCGAAGTC 
GAGGTTATGA AGGACCTCAA TCACCCCAAT ATTGTTCGTC TTTACGAGGT CATCAACGAC 
CCCAAGGCGA ACTCCCTCTT CCTGGTCCTA CAGTACGTGG ATAACGGCGC TGTCGCGCAG 
CTCGACTCGA CTGGACATAT TCGAGCTCCG TTGCACCCGT GGACCGTTCT GCCGATCGCA 
GCGCAAATCA GTGACGGTCT CGTGTACCTT CATGAGCAGC ATATTGTGCA CCGTGATATC 
AAGCCCGAAA ACATCCTCGT TAACCGCGAC GGGCACGCCT TTCTCGCCGA CTTCGGTGTC 
GCAGAGCTCA TGAGCGCGAA GGCAGGCCAA CCAACTGCCG CCACGCTCAC CTATCAAGGC 
ACACCACTGT TCATGGCTCC TGAAATCTAC GCCGGCGTCG ACGACGAAGA CAGAGACGAG 
GATGCGGAGC AAGCCCCAGG TCGGTGCACA CGGCGGAGAA GCAGCAATGA TGCTGACGAG 
AGCAGCAACA TGACGGCGAG CCCCTTGTGC GAGGAGCGGC GCGACTCACG CGTGATCGAC 
CCGTTTGCCC TCGACGTCTG GGCGCTAGGG GTCACCTTCT ACACACTGCT CATCGGCCAC 
GTGCCCTTCA CATCCATGCT CCAGATCTCG CAGACGGTTG AGAAGGGTGT GAACATACCA 
GCCTCGCTCC CAGAGCAGTG GCGCACCGTG CTGCGGCGCA TGATGGAGCC GCGTCAAGAG 
CTGCGGATCT CCTCAGCTGA GCTGTGCCAC ATGCTGCACG CGATGCTAGC GGAGCAAGAG 
GCCGCAGAGG TGAGGGCTGG CGGACGCAGC CGGAAGGCAT CGCTCAGCAC ACGCTCACGA 
AGCGCAACCC TCGGCAATCC TCGCAAAGCC TCTCACCAGT GTTGCGCCGA GGGTCGAATG 
GCGCGCAGTG CAAGTCTTGC TGCTGACGCG GAAGAGGAGA GTATGAACCT TGGCAGCAGC 
AGCAGCAGCG ATGAGAGCGG CACAAGCGGG TACAGCAAGG GCATCCTCGG CCTTAGCATC 
ACATCCGTCG GCCTCAACAT CCTGCGACCG ACACGAATCC GAAAAAAACA GGAGCTGAGT 
TGCAGGGAGA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_11_0250 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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