pde3b cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	pde3b
Entrez Gene ID	101071965
Full Name	phosphodiesterase 3B
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003969958.2	XP_003970007.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B
XM_029845817.1	XP_029701677.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B

Pathways from BioSystems
Gene Ontology

The following pde3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo26527	XM_003969958.2 Latest version!	Takifugu rubripes phosphodiesterase 3B, cGMP-inhibited (pde3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo47233	XM_029845817.1 Latest version!	Takifugu rubripes phosphodiesterase 3B (pde3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pde3b ( XM_003969958.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003970007.1 Takifugu rubripes phosphodiesterase 3B, cGMP-inhibited (pde3b), mRNA.

Price & Availability↑

CloneID	OTo26527
Clone ID Related Accession (Same CDS sequence)	XM_003969958.2
Accession Version	XM_003969958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071066.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003969958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCTGT GGGACTGGGA CCTGAAACAC TGGTACAAGC CGCAGTATCA GGGTGCGTCG 
ACTGGCAACG GCGTGGACCT TTCGGTCATC AACGAGGCCA GGAACCTGGT GTCGGACCTG 
CTGATGGACT CGTCTCTGCC GCCACAGAGC GTCTCCACCC TGCGCAGCAT CTCCAGCCTG 
ATGGGGACCT TCTCGGGCTC GTGCCGCCCC AGGGTCAACC CCTTCACGCC GTTCCCCGGG 
TTCTACCCCT GCGACGAGGT GGAAGACTCC GCCGAGAGGA GCGAGAGGAA GAGCATCAAG 
GGTCTGAGCA GCAGGAACAG CCTCCCCACC CCTCAGCCCA GACGCAGCTC CACCTCCTCC 
TCGTGCTCCA CGCTCCCGTC TCTGGACTCG GCCTCGGCAC GTTGGGAGCG TAACACTGGC 
AAGAGATCCC ACCCTGACCT CAATTTAAGC AGCCCAGGTC TGTCAAATGG ACCCAATGCA 
AATCTGCTGA CCATCCCTAA GCAGAGGTCC TCGTCTGTTA CTCTGACCTA CCACGCGGGG 
CCCCGGAGAT CAGGCGCTTC TCCAAGCCTG AGCCCAGTGG CTTCGCCGGC CCACAGCCCT 
GTAGCCATGG GCATGGGCTC CACGTCGTCC CTCATCCGCC CCCCCGTGGA GTTCCCCGAC 
ACGGCCGACT TCCTGACAAA ACCCAGCGTC AGTCTCAACT TACACAAACC TCTGGCCTCG 
ACGCTGAGTT CGCCTGATTT CCAGCAGGCC TTCCGCGGCG CCGCCTTCCC CGTCTGCACC 
AGCTGCGGCC GTCAGATAGC CAAAGGAGGC GATACGAGCC GCCTGCCGTT TTCCAACGAG 
GCTCAGCGCA GACGCTCAGC TGGCGAGGGG CTGGACTTCC AAGGAGAGCC TCTGATCAGG 
GAGGAGAGCG TGGAGATGGA GGCGTCTGCA GACGGGAGGC AGGATGCAGC AGGAAAGCTG 
GTCCAAACAG AGGAGGAGGA GGAGGAGGAG GCGGCGGAGG AGAGCTCAAG CACGGAGCAG 
CCGACTCAGG AAGCAGAGGT CGAGCAGGAG TTCATCCTCG ATCCCGTGCT GGAGGAGCAC 
GAAACCTTCA TGGAGAAGAT GAACACTTGG AACTTCCAGA TCTTTGAGCT GGTGGACAAA 
ACGGGAGGGA AAACCGGCAG AATCCTCAGC TACGTGACAT ACACCCTTTT CCAGGACGCG 
TGTCTGTTTG AAATCTTCAA AATCCCTGTT CGGGAATTCA TGACGTACTT CAGTGCCCTG 
GAAAGTGGCT ACAGGGACAT TCCCTATCAC AACCGGGTCC ACGCCGCTGA CGTGCTCCAC 
GCCGTCTGGT ACCTGACCAC TCGGCCAATC CCCGGCTTCC AGCAGATCCA CAGCGAGCAT 
GTGACAGGAA GTGACACAGA CTCCGACAGC GGCATCTCGC CGGGCAGGAT ATCCTACGCC 
TCCTCCAAAA GCTGCTCCAT TTCAGACGAC AGCTACGGCT GCCTGGCGTG GAACATCCCC 
GCGCTGGAGC TCATGGCGCT CTACGTGGCA GCGGCCATGC ATGACTACGA CCACCCGGGC 
CGCACCAACG CCTTCCTGGT GGCCACGAAC GCGCCTCAGG CTGTGCTCTA CAATGACAGG 
TCCGTGCTGG AGAACCACCA CGCCGCCGCC GCCTGGAGCC TCTACCTTTC CCAGCCAGAA 
TTTAACTTTC TGGTCAACCT GGATCACGTG GAGTTCAAGC GCTTCCGCTT CCTGGTCATT 
GAAGCCATCC TGGCCACGGA CCTGAAGAAG CACTTTGACT TCCTGGCTGA GTTCAACTCT 
AAGGTGAACG ATGTCAACAG TCCCGGGATT GACTGGACCA ATGAGAACGA CAGACTTCTG 
GTGTGCCAGG TGTGCATCAA GCTAGCGGAT ATCAATGGAC CCGCCAAGAT CCGCGACCTG 
CACCTCAAGT GGACAGAAGG CATCGTCAAT GAGTTTTACG AGCAGGGAGA CGAGGAGGCT 
AAACTGGGAT TACCTGTCAG CCCCTTCATG GACCGCTCCT CCCCCCAGCT GGCAAAGCTG 
CAGGAGTCCT TCATCACCCA CATCGTGGGA CCGCTCTGTA ACTCCTACGA CGCCGCAGGC 
CTACTTCCTG GTTACTGGAT CAACCAGGAA GGATCAGACG AGGACGATGA AGAAGGGCAG 
GTGGAGACCG ACACGGATGA AGAGGAGGAC GACGAGATGG AGGACGAGCT GGAACCAAAA 
CGGAGGAAGA GCCGCCGGAG GTTATTCTGC AGCATCATGC AGCACCTGGC GGAGAACCAC 
AAGGTGTGGA AGAAGACCAT CGAGGAGGAG GAGAAGAGCA AAGAGCTCGG CAAACCGCAG 
CAGCAGCCCG ACAGCCTGCC GTCCAGCCTG CCCACCTCCC CGTCAGACGA CATCCAGGTC 
ATCCAGGAGG AGGAGGAGGG CGAGGAGCGT CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003970007.1
CDS	652..3087
Translation	MILWDWDLKHWYKPQYQGASTGNGVDLSVINEARNLVSDLLMDSSLPPQSVSTLRSISSLMGTFSGSCRPRVNPFTPFPGFYPCDEVEDSAERSERKSIKGLSSRNSLPTPQPRRSSTSSSCSTLPSLDSASARWERNTGKRSHPDLNLSSPGLSNGPNANLLTIPKQRSSSVTLTYHAGPRRSGASPSLSPVASPAHSPVAMGMGSTSSLIRPPVEFPDTADFLTKPSVSLNLHKPLASTLSSPDFQQAFRGAAFPVCTSCGRQIAKGGDTSRLPFSNEAQRRRSAGEGLDFQGEPLIREESVEMEASADGRQDAAGKLVQTEEEEEEEAAEESSSTEQPTQEAEVEQEFILDPVLEEHETFMEKMNTWNFQIFELVDKTGGKTGRILSYVTYTLFQDACLFEIFKIPVREFMTYFSALESGYRDIPYHNRVHAADVLHAVWYLTTRPIPGFQQIHSEHVTGSDTDSDSGISPGRISYASSKSCSISDDSYGCLAWNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAAAAWSLYLSQPEFNFLVNLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNSKVNDVNSPGIDWTNENDRLLVCQVCIKLADINGPAKIRDLHLKWTEGIVNEFYEQGDEEAKLGLPVSPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGYWINQEGSDEDDEEGQVETDTDEEEDDEMEDELEPKRRKSRRRLFCSIMQHLAENHKVWKKTIEEEEKSKELGKPQQQPDSLPSSLPTSPSDDIQVIQEEEEGEERP

Target ORF information:

RefSeq Version	XM_003969958.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphodiesterase 3B, cGMP-inhibited (pde3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003969958.2

ORF Insert Sequence:

ATGATCCTGT GGGACTGGGA CCTGAAACAC TGGTACAAGC CGCAGTATCA GGGTGCGTCG 
ACTGGCAACG GCGTGGACCT TTCGGTCATC AACGAGGCCA GGAACCTGGT GTCGGACCTG 
CTGATGGACT CGTCTCTGCC GCCACAGAGC GTCTCCACCC TGCGCAGCAT CTCCAGCCTG 
ATGGGGACCT TCTCGGGCTC GTGCCGCCCC AGGGTCAACC CCTTCACGCC GTTCCCCGGG 
TTCTACCCCT GCGACGAGGT GGAAGACTCC GCCGAGAGGA GCGAGAGGAA GAGCATCAAG 
GGTCTGAGCA GCAGGAACAG CCTCCCCACC CCTCAGCCCA GACGCAGCTC CACCTCCTCC 
TCGTGCTCCA CGCTCCCGTC TCTGGACTCG GCCTCGGCAC GTTGGGAGCG TAACACTGGC 
AAGAGATCCC ACCCTGACCT CAATTTAAGC AGCCCAGGTC TGTCAAATGG ACCCAATGCA 
AATCTGCTGA CCATCCCTAA GCAGAGGTCC TCGTCTGTTA CTCTGACCTA CCACGCGGGG 
CCCCGGAGAT CAGGCGCTTC TCCAAGCCTG AGCCCAGTGG CTTCGCCGGC CCACAGCCCT 
GTAGCCATGG GCATGGGCTC CACGTCGTCC CTCATCCGCC CCCCCGTGGA GTTCCCCGAC 
ACGGCCGACT TCCTGACAAA ACCCAGCGTC AGTCTCAACT TACACAAACC TCTGGCCTCG 
ACGCTGAGTT CGCCTGATTT CCAGCAGGCC TTCCGCGGCG CCGCCTTCCC CGTCTGCACC 
AGCTGCGGCC GTCAGATAGC CAAAGGAGGC GATACGAGCC GCCTGCCGTT TTCCAACGAG 
GCTCAGCGCA GACGCTCAGC TGGCGAGGGG CTGGACTTCC AAGGAGAGCC TCTGATCAGG 
GAGGAGAGCG TGGAGATGGA GGCGTCTGCA GACGGGAGGC AGGATGCAGC AGGAAAGCTG 
GTCCAAACAG AGGAGGAGGA GGAGGAGGAG GCGGCGGAGG AGAGCTCAAG CACGGAGCAG 
CCGACTCAGG AAGCAGAGGT CGAGCAGGAG TTCATCCTCG ATCCCGTGCT GGAGGAGCAC 
GAAACCTTCA TGGAGAAGAT GAACACTTGG AACTTCCAGA TCTTTGAGCT GGTGGACAAA 
ACGGGAGGGA AAACCGGCAG AATCCTCAGC TACGTGACAT ACACCCTTTT CCAGGACGCG 
TGTCTGTTTG AAATCTTCAA AATCCCTGTT CGGGAATTCA TGACGTACTT CAGTGCCCTG 
GAAAGTGGCT ACAGGGACAT TCCCTATCAC AACCGGGTCC ACGCCGCTGA CGTGCTCCAC 
GCCGTCTGGT ACCTGACCAC TCGGCCAATC CCCGGCTTCC AGCAGATCCA CAGCGAGCAT 
GTGACAGGAA GTGACACAGA CTCCGACAGC GGCATCTCGC CGGGCAGGAT ATCCTACGCC 
TCCTCCAAAA GCTGCTCCAT TTCAGACGAC AGCTACGGCT GCCTGGCGTG GAACATCCCC 
GCGCTGGAGC TCATGGCGCT CTACGTGGCA GCGGCCATGC ATGACTACGA CCACCCGGGC 
CGCACCAACG CCTTCCTGGT GGCCACGAAC GCGCCTCAGG CTGTGCTCTA CAATGACAGG 
TCCGTGCTGG AGAACCACCA CGCCGCCGCC GCCTGGAGCC TCTACCTTTC CCAGCCAGAA 
TTTAACTTTC TGGTCAACCT GGATCACGTG GAGTTCAAGC GCTTCCGCTT CCTGGTCATT 
GAAGCCATCC TGGCCACGGA CCTGAAGAAG CACTTTGACT TCCTGGCTGA GTTCAACTCT 
AAGGTGAACG ATGTCAACAG TCCCGGGATT GACTGGACCA ATGAGAACGA CAGACTTCTG 
GTGTGCCAGG TGTGCATCAA GCTAGCGGAT ATCAATGGAC CCGCCAAGAT CCGCGACCTG 
CACCTCAAGT GGACAGAAGG CATCGTCAAT GAGTTTTACG AGCAGGGAGA CGAGGAGGCT 
AAACTGGGAT TACCTGTCAG CCCCTTCATG GACCGCTCCT CCCCCCAGCT GGCAAAGCTG 
CAGGAGTCCT TCATCACCCA CATCGTGGGA CCGCTCTGTA ACTCCTACGA CGCCGCAGGC 
CTACTTCCTG GTTACTGGAT CAACCAGGAA GGATCAGACG AGGACGATGA AGAAGGGCAG 
GTGGAGACCG ACACGGATGA AGAGGAGGAC GACGAGATGG AGGACGAGCT GGAACCAAAA 
CGGAGGAAGA GCCGCCGGAG GTTATTCTGC AGCATCATGC AGCACCTGGC GGAGAACCAC 
AAGGTGTGGA AGAAGACCAT CGAGGAGGAG GAGAAGAGCA AAGAGCTCGG CAAACCGCAG 
CAGCAGCCCG ACAGCCTGCC GTCCAGCCTG CCCACCTCCC CGTCAGACGA CATCCAGGTC 
ATCCAGGAGG AGGAGGAGGG CGAGGAGCGT CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde3b ( XM_029845817.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029701677.1 Takifugu rubripes phosphodiesterase 3B (pde3b), mRNA.

Price & Availability↑

CloneID	OTo47233
Clone ID Related Accession (Same CDS sequence)	XM_029845817.1
Accession Version	XM_029845817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042297.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC GGGAGGAAGG AGAGGGTTTG CACGGCAAAA TCAGGCACCA CGACGGAGCT 
CGGAGTGTAA ACAGCTCGGA AGGCTTCAGG AACGGCTATG TGAAGAGCTG CGTCACCCCT 
CTGAAACAGG ACCGTCAGAG GGGGTTTTTG GTGAGCGTGT GCAGCTTGTG CAACCAGGAC 
ATCGTGAGCT CCAAACTGCT GAAGGTCTCC GCGCTCTACG CCGCTGCGCT GGCAGTCTTG 
GCGCTTTCTT TCCTCGTTTC CAGGAGCTTG CAAAGTGACC CGCTGTGTGA TCTGCGGACT 
TTCCTGTCGG GCTTCTCCCA GTCCATCAGT CCTTTATTCA GCATCGGGTG CGCGTTCTTC 
TTCCTGACCT TCTACCTGCG GAGGAAGAAG AAGGAGAGCA GCAAATGCTG GCTGGCGTCG 
CTGCCAGCTT CGTGCTACCT CGGGGATTTT TTGGTGTTGC GGGTCCTGCA ATGGGGAGAG 
GAGCAGCACC AGATGCAAAT GGTGGGCAGG TTGTTGTTCG TGCTGGGCTG CGTGGGTCTG 
CTCACGCTCA TCCCCACTCT CAAACTCAAA CACAGTGTCC TCGTCCTGGT TTTTGCCAGC 
GTGGTTTGGC TGGTGTCGCT CACCAGTTTG AGCGCCCTGC CCGCGCTGCT CAGACCGCTG 
GCCGCCTGCC TCTCCGGGGT CTCCGGCTCC CTGCTGGCGC TCTGGTTCGA CCGCTGCTGC 
CCGCCGAGGG ACCCGCCGAG TGGGACCTCC TCCGCCGAGG AGAAGGTGCC CGTGATCCGG 
CTGAGGAGAC GGTCCAGCTG CGTGTCCCTG GGTGAAACCT CCACCAGCTA TTATGGCAGC 
TGTAAAATGC CCCGCAGACC TTCGTTACCC TGCATATCCA GGGAACAGAT GATCCTGTGG 
GACTGGGACC TGAAACACTG GTACAAGCCG CAGTATCAGG GTGCGTCGAC TGGCAACGGC 
GTGGACCTTT CGGTCATCAA CGAGGCCAGG AACCTGGTGT CGGACCTGCT GATGGACTCG 
TCTCTGCCGC CACAGAGCGT CTCCACCCTG CGCAGCATCT CCAGCCTGAT GGGGACCTTC 
TCGGGCTCGT GCCGCCCCAG GGTCAACCCC TTCACGCCGT TCCCCGGGTT CTACCCCTGC 
GACGAGGTGG AAGACTCCGC CGAGAGGAGC GAGAGGAAGA GCATCAAGGG TCTGAGCAGC 
AGGAACAGCC TCCCCACCCC TCAGCCCAGA CGCAGCTCCA CCTCCTCCTC GTGCTCCACG 
CTCCCGTCTC TGGACTCGGC CTCGGCACGT TGGGAGCGTA ACACTGGCAA GAGATCCCAC 
CCTGACCTCA ATTTAAGCAG CCCAGGTCTG TCAAATGGAC CCAATGCAAA TCTGCTGACC 
ATCCCTAAGC AGAGGTCCTC GTCTGTTACT CTGACCTACC ACGCGGGGCC CCGGAGATCA 
GGCGCTTCTC CAAGCCTGAG CCCAGTGGCT TCGCCGGCCC ACAGCCCTGT AGCCATGGGC 
ATGGGCTCCA CGTCGTCCCT CATCCGCCCC CCCGTGGAGT TCCCCGACAC GGCCGACTTC 
CTGACAAAAC CCAGCGTCAG TCTCAACTTA CACAAACCTC TGGCCTCGAC GCTGAGTTCG 
CCTGATTTCC AGCAGGCCTT CCGCGGCGCC GCCTTCCCCG TCTGCACCAG CTGCGGCCGT 
CAGATAGCCA AAGGAGGCGA TACGAGCCGC CTGCCGTTTT CCAACGAGGC TCAGCGCAGA 
CGCTCAGCTG GCGAGGGGCT GGACTTCCAA GGAGAGCCTC TGATCAGGGA GGAGAGCGTG 
GAGATGGAGG CGTCTGCAGA CGGGAGGCAG GATGCAGCAG GAAAGCTGGT CCAAACAGAG 
GAGGAGGAGG AGGAGGAGGA GGCGGCGGAG GAGAGCTCAA GCACGGAGCA GCCGACTCAG 
GAAGCAGAGG TCGAGCAGGA GTTCATCCTC GATCCCGTGC TGGAGGAGCA CGAAACCTTC 
ATGGAGAAGA TGAACACTTG GAACTTCCAG ATCTTTGAGC TGGTGGACAA AACGGGAGGG 
AAAACCGGCA GAATCCTCAG CTACGTGACA TACACCCTTT TCCAGGACGC GTGTCTGTTT 
GAAATCTTCA AAATCCCTGT TCGGGAATTC ATGACGTACT TCAGTGCCCT GGAAAGTGGC 
TACAGGGACA TTCCCTATCA CAACCGGGTC CACGCCGCTG ACGTGCTCCA CGCCGTCTGG 
TACCTGACCA CTCGGCCAAT CCCCGGCTTC CAGCAGATCC ACAGCGAGCA TGTGACAGGA 
AGTGACACAG ACTCCGACAG CGGCATCTCG CCGGGCAGGA TATCCTACGC CTCCTCCAAA 
AGCTGCTCCA TTTCAGACGA CAGCTACGGC TGCCTGGCGT GGAACATCCC CGCGCTGGAG 
CTCATGGCGC TCTACGTGGC AGCGGCCATG CACGACTACG ACCACCCGGG CCGCACCAAC 
GCCTTCCTGG TGGCCACGAA CGCGCCTCAG GCTGTGCTCT ACAATGACAG GTCCGTGCTG 
GAGAACCACC ACGCCGCCGC CGCCTGGAGC CTCTACCTTT CCCAGCCAGA ATTTAACTTT 
CTGGTCAACC TGGATCACGT GGAGTTCAAG CGCTTCCGCT TCCTGGTCAT TGAAGCCATC 
CTGGCCACGG ACCTGAAGAA GCACTTTGAC TTCCTGGCTG AGTTCAACTC TAAGGTGAAC 
GATGTCAACA GTCCCGGGAT TGACTGGACC AATGAGAACG ACAGACTTCT GGTGTGCCAG 
GTGTGCATCA AGCTAGCGGA TATCAATGGA CCCGCCAAGA TCCGCGACCT GCACCTCAAG 
TGGACAGAAG GCATCGTCAA TGAGTTTTAC GAGCAGGGAG ACGAGGAGGC TAAACTGGGA 
TTACCTGTCA GCCCCTTCAT GGACCGCTCC TCCCCCCAGC TGGCAAAGCT GCAGGAGTCC 
TTCATCACCC ACATCGTGGG ACCGCTCTGT AACTCCTACG ACGCCGCAGG CCTACTTCCT 
GGTTACTGGA TCAACCAGGA AGGATCAGAC GAGGACGATG AAGAAGGGCA GGTGGAGACC 
GACACGGATG AAGAGGAGGA CGACGAGATG GAGGACGAGC TGGAACCAAA ACGGAGGAAG 
AGCCGCCGGA GGTTATTCTG CAGCATCATG CAGCACCTGG CGGAGAACCA CAAGGTGTGG 
AAGAAGACCA TCGAGGAGGA GGAGAAGAGC AAAGAGCTCG GCAAACCGCA GCAGCAGCCC 
GACAGCCTGC CGTCCAGCCT GCCCACCTCC CCGTCAGACG ACATCCAGGT CATCCAGGAG 
GAGGAGGAGG GCGAGGAGCG TCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029701677.1
CDS	166..3492
Translation	MAAREEGEGLHGKIRHHDGARSVNSSEGFRNGYVKSCVTPLKQDRQRGFLVSVCSLCNQDIVSSKLLKVSALYAAALAVLALSFLVSRSLQSDPLCDLRTFLSGFSQSISPLFSIGCAFFFLTFYLRRKKKESSKCWLASLPASCYLGDFLVLRVLQWGEEQHQMQMVGRLLFVLGCVGLLTLIPTLKLKHSVLVLVFASVVWLVSLTSLSALPALLRPLAACLSGVSGSLLALWFDRCCPPRDPPSGTSSAEEKVPVIRLRRRSSCVSLGETSTSYYGSCKMPRRPSLPCISREQMILWDWDLKHWYKPQYQGASTGNGVDLSVINEARNLVSDLLMDSSLPPQSVSTLRSISSLMGTFSGSCRPRVNPFTPFPGFYPCDEVEDSAERSERKSIKGLSSRNSLPTPQPRRSSTSSSCSTLPSLDSASARWERNTGKRSHPDLNLSSPGLSNGPNANLLTIPKQRSSSVTLTYHAGPRRSGASPSLSPVASPAHSPVAMGMGSTSSLIRPPVEFPDTADFLTKPSVSLNLHKPLASTLSSPDFQQAFRGAAFPVCTSCGRQIAKGGDTSRLPFSNEAQRRRSAGEGLDFQGEPLIREESVEMEASADGRQDAAGKLVQTEEEEEEEEAAEESSSTEQPTQEAEVEQEFILDPVLEEHETFMEKMNTWNFQIFELVDKTGGKTGRILSYVTYTLFQDACLFEIFKIPVREFMTYFSALESGYRDIPYHNRVHAADVLHAVWYLTTRPIPGFQQIHSEHVTGSDTDSDSGISPGRISYASSKSCSISDDSYGCLAWNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAAAAWSLYLSQPEFNFLVNLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNSKVNDVNSPGIDWTNENDRLLVCQVCIKLADINGPAKIRDLHLKWTEGIVNEFYEQGDEEAKLGLPVSPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGYWINQEGSDEDDEEGQVETDTDEEEDDEMEDELEPKRRKSRRRLFCSIMQHLAENHKVWKKTIEEEEKSKELGKPQQQPDSLPSSLPTSPSDDIQVIQEEEEGEERP

Target ORF information:

RefSeq Version	XM_029845817.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphodiesterase 3B (pde3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029845817.1

ORF Insert Sequence:

ATGGCTGCAC GGGAGGAAGG AGAGGGTTTG CACGGCAAAA TCAGGCACCA CGACGGAGCT 
CGGAGTGTAA ACAGCTCGGA AGGCTTCAGG AACGGCTATG TGAAGAGCTG CGTCACCCCT 
CTGAAACAGG ACCGTCAGAG GGGGTTTTTG GTGAGCGTGT GCAGCTTGTG CAACCAGGAC 
ATCGTGAGCT CCAAACTGCT GAAGGTCTCC GCGCTCTACG CCGCTGCGCT GGCAGTCTTG 
GCGCTTTCTT TCCTCGTTTC CAGGAGCTTG CAAAGTGACC CGCTGTGTGA TCTGCGGACT 
TTCCTGTCGG GCTTCTCCCA GTCCATCAGT CCTTTATTCA GCATCGGGTG CGCGTTCTTC 
TTCCTGACCT TCTACCTGCG GAGGAAGAAG AAGGAGAGCA GCAAATGCTG GCTGGCGTCG 
CTGCCAGCTT CGTGCTACCT CGGGGATTTT TTGGTGTTGC GGGTCCTGCA ATGGGGAGAG 
GAGCAGCACC AGATGCAAAT GGTGGGCAGG TTGTTGTTCG TGCTGGGCTG CGTGGGTCTG 
CTCACGCTCA TCCCCACTCT CAAACTCAAA CACAGTGTCC TCGTCCTGGT TTTTGCCAGC 
GTGGTTTGGC TGGTGTCGCT CACCAGTTTG AGCGCCCTGC CCGCGCTGCT CAGACCGCTG 
GCCGCCTGCC TCTCCGGGGT CTCCGGCTCC CTGCTGGCGC TCTGGTTCGA CCGCTGCTGC 
CCGCCGAGGG ACCCGCCGAG TGGGACCTCC TCCGCCGAGG AGAAGGTGCC CGTGATCCGG 
CTGAGGAGAC GGTCCAGCTG CGTGTCCCTG GGTGAAACCT CCACCAGCTA TTATGGCAGC 
TGTAAAATGC CCCGCAGACC TTCGTTACCC TGCATATCCA GGGAACAGAT GATCCTGTGG 
GACTGGGACC TGAAACACTG GTACAAGCCG CAGTATCAGG GTGCGTCGAC TGGCAACGGC 
GTGGACCTTT CGGTCATCAA CGAGGCCAGG AACCTGGTGT CGGACCTGCT GATGGACTCG 
TCTCTGCCGC CACAGAGCGT CTCCACCCTG CGCAGCATCT CCAGCCTGAT GGGGACCTTC 
TCGGGCTCGT GCCGCCCCAG GGTCAACCCC TTCACGCCGT TCCCCGGGTT CTACCCCTGC 
GACGAGGTGG AAGACTCCGC CGAGAGGAGC GAGAGGAAGA GCATCAAGGG TCTGAGCAGC 
AGGAACAGCC TCCCCACCCC TCAGCCCAGA CGCAGCTCCA CCTCCTCCTC GTGCTCCACG 
CTCCCGTCTC TGGACTCGGC CTCGGCACGT TGGGAGCGTA ACACTGGCAA GAGATCCCAC 
CCTGACCTCA ATTTAAGCAG CCCAGGTCTG TCAAATGGAC CCAATGCAAA TCTGCTGACC 
ATCCCTAAGC AGAGGTCCTC GTCTGTTACT CTGACCTACC ACGCGGGGCC CCGGAGATCA 
GGCGCTTCTC CAAGCCTGAG CCCAGTGGCT TCGCCGGCCC ACAGCCCTGT AGCCATGGGC 
ATGGGCTCCA CGTCGTCCCT CATCCGCCCC CCCGTGGAGT TCCCCGACAC GGCCGACTTC 
CTGACAAAAC CCAGCGTCAG TCTCAACTTA CACAAACCTC TGGCCTCGAC GCTGAGTTCG 
CCTGATTTCC AGCAGGCCTT CCGCGGCGCC GCCTTCCCCG TCTGCACCAG CTGCGGCCGT 
CAGATAGCCA AAGGAGGCGA TACGAGCCGC CTGCCGTTTT CCAACGAGGC TCAGCGCAGA 
CGCTCAGCTG GCGAGGGGCT GGACTTCCAA GGAGAGCCTC TGATCAGGGA GGAGAGCGTG 
GAGATGGAGG CGTCTGCAGA CGGGAGGCAG GATGCAGCAG GAAAGCTGGT CCAAACAGAG 
GAGGAGGAGG AGGAGGAGGA GGCGGCGGAG GAGAGCTCAA GCACGGAGCA GCCGACTCAG 
GAAGCAGAGG TCGAGCAGGA GTTCATCCTC GATCCCGTGC TGGAGGAGCA CGAAACCTTC 
ATGGAGAAGA TGAACACTTG GAACTTCCAG ATCTTTGAGC TGGTGGACAA AACGGGAGGG 
AAAACCGGCA GAATCCTCAG CTACGTGACA TACACCCTTT TCCAGGACGC GTGTCTGTTT 
GAAATCTTCA AAATCCCTGT TCGGGAATTC ATGACGTACT TCAGTGCCCT GGAAAGTGGC 
TACAGGGACA TTCCCTATCA CAACCGGGTC CACGCCGCTG ACGTGCTCCA CGCCGTCTGG 
TACCTGACCA CTCGGCCAAT CCCCGGCTTC CAGCAGATCC ACAGCGAGCA TGTGACAGGA 
AGTGACACAG ACTCCGACAG CGGCATCTCG CCGGGCAGGA TATCCTACGC CTCCTCCAAA 
AGCTGCTCCA TTTCAGACGA CAGCTACGGC TGCCTGGCGT GGAACATCCC CGCGCTGGAG 
CTCATGGCGC TCTACGTGGC AGCGGCCATG CACGACTACG ACCACCCGGG CCGCACCAAC 
GCCTTCCTGG TGGCCACGAA CGCGCCTCAG GCTGTGCTCT ACAATGACAG GTCCGTGCTG 
GAGAACCACC ACGCCGCCGC CGCCTGGAGC CTCTACCTTT CCCAGCCAGA ATTTAACTTT 
CTGGTCAACC TGGATCACGT GGAGTTCAAG CGCTTCCGCT TCCTGGTCAT TGAAGCCATC 
CTGGCCACGG ACCTGAAGAA GCACTTTGAC TTCCTGGCTG AGTTCAACTC TAAGGTGAAC 
GATGTCAACA GTCCCGGGAT TGACTGGACC AATGAGAACG ACAGACTTCT GGTGTGCCAG 
GTGTGCATCA AGCTAGCGGA TATCAATGGA CCCGCCAAGA TCCGCGACCT GCACCTCAAG 
TGGACAGAAG GCATCGTCAA TGAGTTTTAC GAGCAGGGAG ACGAGGAGGC TAAACTGGGA 
TTACCTGTCA GCCCCTTCAT GGACCGCTCC TCCCCCCAGC TGGCAAAGCT GCAGGAGTCC 
TTCATCACCC ACATCGTGGG ACCGCTCTGT AACTCCTACG ACGCCGCAGG CCTACTTCCT 
GGTTACTGGA TCAACCAGGA AGGATCAGAC GAGGACGATG AAGAAGGGCA GGTGGAGACC 
GACACGGATG AAGAGGAGGA CGACGAGATG GAGGACGAGC TGGAACCAAA ACGGAGGAAG 
AGCCGCCGGA GGTTATTCTG CAGCATCATG CAGCACCTGG CGGAGAACCA CAAGGTGTGG 
AAGAAGACCA TCGAGGAGGA GGAGAAGAGC AAAGAGCTCG GCAAACCGCA GCAGCAGCCC 
GACAGCCTGC CGTCCAGCCT GCCCACCTCC CCGTCAGACG ACATCCAGGT CATCCAGGAG 
GAGGAGGAGG GCGAGGAGCG TCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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pde3b cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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