wnk4 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	wnk4
Entrez Gene ID	101066766
Full Name	WNK lysine deficient protein kinase 4
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011617375.1	XP_011615677.1	serine/threonine-protein kinase WNK4 isoform X2
XM_029850413.1	XP_029706273.1	serine/threonine-protein kinase WNK4 isoform X3
XM_011617375.2	XP_011615677.2	serine/threonine-protein kinase WNK4 isoform X2
XM_029850410.1	XP_029706270.1	serine/threonine-protein kinase WNK4 isoform X3
XM_011616884.2	XP_011615186.2	serine/threonine-protein kinase WNK4 isoform X1
XM_011616884.1	XP_011615186.1	serine/threonine-protein kinase WNK4 isoform X1

Pathways from BioSystems
Gene Ontology

The following wnk4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnk4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo22775	XM_011616884.1 Latest version!	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo22776	XM_011617375.1 Latest version!	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo43121	XM_011616884.2 Latest version!	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo43122	XM_011617375.2 Latest version!	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo43123	XM_029850410.1 Latest version!	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo43123	XM_029850413.1 Latest version!	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wnk4 ( XM_011616884.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011615186.1 Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo22775
Clone ID Related Accession (Same CDS sequence)	XM_011616884.1
Accession Version	XM_011616884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	serine/threonine-protein kinase WNK4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070896.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACACG CACAGCTTGA GATAGTAGGA CGCGGCTTCC CGGGACACCC TATGGACATG 
ACCGACGACT GCTTATCCCC CGTCCCATCC GTGCTGAGGG CGCACGGTTT GGGCGCACGG 
AGGAACTCGT ACGGTCTGCA TAAAATCAGC ATGGACATCG ATTCTCCTCT GTACAGCGGC 
ACACTTTCCA AAGCCTTGTC TTGGTCTGCT GAGAACATTC TGACACTGTC CGAGTCCAGA 
GTAGAGGAGG AAAAAAACGA CTACTCCCCG CCGTCTCCGT CGCCGGTGTC CAGCCCGGCC 
ACCGGCTCCA TCACGCCCCC CTGCAGCCAG GAGCCAGACG CCGCCGCGCA GGGATCCAGC 
GCACAGAGTT CGGAGTCTGA AAACGAAGTT CAGAATAAGC AGCAAAAGAT CCTCTCTCGC 
GTCAAGTTCG ATGCTCAGTG GGAGCAGGAC GAGAAAGAAG ACGTCGAGAC CAAAGCGGTG 
GCGACTTCGC CCGATGGAAG ATACCTCAAA TTCAACATTG AGATCGGGAG GGGCTCGTTC 
AAGACGGTCT ATAAGGGACT GGACACTGAA ACGACGGTGG AAGTGGCGTG GTGTGAGTTA 
CAGACTCGGA AGCTGACCAA GGCGGAGCGG CAGCGTTTCA GCGAGGAGGT GGAAATGCTG 
AAGTGCCTGC AGCACCCCAA CATCGTCCGC TTCCACGACT CCTGGAAGTC GACGGTGAAG 
GGCCTCAAAT GCATCATCCT GGTGACGGAA CTGATGACGT CGGGCACGCT CAAAACGTAC 
TTGAAGAGAT TCAAGGAGAT GAAGCTAAAG CTGCTCCAGC GTTGGAGTCG ACAAATCCTC 
AAAGGGCTTC ACTTCCTGCA CACGCGAACG CCTCCCATCA TCCACAGAGA CCTGAAGTGT 
GACAACATCT TCATCACGGG CCCCACTGGC TCTGTCAAGA TTGGAGATCT GGGACTTGCC 
ACTCTTAAAA GTGCCTCTTT TGCTAAAAGT GTCATTGGAA CGCCAGAGTT CATGGCCCCG 
GAGATGTACG AGGAGAAGTA CGACGAAGCG GTGGACGTCT ACGCCTTCGG AATGTGCATC 
CTGGAGATGG CCACCTCTGA GTATCCGTAC TCCGAGTGCC AAAACGCCGC CCAGATCTAC 
CGCAAAGTCA CCAGTGGAAT TAAACCAGAC AGTTTCTACA AAGTCAAAGT CCCGGAGCTC 
AAAGAGATCA TCGGGGGCTG CATTCGCATG AACAACGATG AAAGGTACAC CATTCAGGAC 
CTCTTGGATC ACCCCTTCTT CCAGGAGAAC AACGGCGTGC ACGTGGAGCT GGCGGAGGAG 
GACGACATGG TGAAGCCAGG CCTGAAGCTG TGGCTGCGCA TGGACGACAC AAAGAAGCTC 
CACGGGAAGT ACAAGGACAA CAACGCCATC GAGTTCCTCT TTGAGCTCTA CAAAGACGTG 
CCCGAGGAGG TGGCTCAGGA GATGGTGGTG CTCGGGTTCG TGTGCGAAGC CGACTTCAAA 
ATGGTGGCCA AGGCCATACG GGACAGGGTG ACAGCCATCA AGCGGCAAAG GGAGAAGATG 
AGGAAGCAGG CGGAGGAGCA TAAGAAGAAG CAGCAGCAGG AAGCCATAGA AGAAGAGCCG 
GAACCTCCGC CTGTTTTGCC CAAAGTTTTC GACGCCGTCC CGGTGGAGTC TCCACCCCAA 
GCCCTAACCC CACTGGCTCC CATCCTGTCC CCGGTCACCA GCTCCGTGGA CTCTGGCGTC 
AGCTCCAGCT ACCCCGCAGA GCCGGAGGAG CCTGAGGCCG ACCAGCACTT CCACATGCGC 
CACAACAGCC TGTCATCTGC TAACTCTGAC TGCGAGACAG ACGGCTATCT GAGCTCCTCT 
GGTCTTCAGG ATCCACTGGA GGCCGCCAGC TTTAACTCGC AGGCCACACC TCCTGCCACA 
TACAACGAAG GCGCACCAAC TGGAAACGGT TTCCCCATCG CGGCTCTGCG CTTCCCCTCA 
AGCATCGCTG TATCCAACAG CATAGAGAAC GGAAACTCGA GATCTCCGAG TGGCCTCAAC 
TCACCTGTGG ACAGTTACGC CTCTGACGTG ACCTCGGGTT TGAGTGATGG CTACGAGGGC 
CTATCGGAGA AGAGCGAGAA AACGGCCGCC AGGAAAACTG CTGGGAAGAT GTTCAGAAGG 
AGGGCGCGAT CAAGGCTGCG CATCACAGGG CTCTCTGATA AAGTGGACCG GGTGGTGGAG 
TGCCAGCTGC AGACGCACAA CGACAAGATG GTGACGTTCA AGTTCGACCT GGACGGAGAC 
AATCCCGAAG ACATCGCCAC TGTCATGGTG CACAATGAGT TCATCCTGCC GTCGGAGAAA 
GAGGGCTTCA TCCACCGTAT GGGGGACATC ATCAAGCGTG CAGAGTCTCT GATGGCCAAA 
GGGCAGCCAG GCCACGCCAG CGTGCACCGC CTCACACAGC CCGCTTTCCA CAGTGGGGCT 
AATTCACTGT CTTCGTCACA GCCTAATCTG CACAGCCACA ACCGCCCTCG CACCAGCTCC 
TCCTCATCTC TACCTGACTT TAGCGTGGCT AACCCAAGAG CTGGGCTGGG TGGCCCCAGT 
GGCGACATCT TCGGTGCAGA CATTACTTCA CCTGAGCGAC CTTTAATACG CTCACAGTCT 
TTCCACAACA GCCCAGGTTC TCCTCTCCAT CATCACCACC ACTACCATCC TGCACCTGCC 
CTGCCGCACC ACCAGCATTA CCACTTGCCT TACAGTAACT TCCCATTCCC GTACCCCGGC 
TCTCCAGGCT CCCCCAAGCC GGCCCACCAC CCACCGCTCA CACGTGTAGC CAGTAACCCC 
ACCTTCCCTT CTGTTCACTC CCCTGCCCCC GGTTCGCCCA ATTCCCTCAA CGAGGCCCCG 
CATCCAGGTA GCAACGACAG TATCACCCTG TCCCCACCTG TGACTCAGCA TCCCAACATG 
TGGCCCCCCC ACACGCAGCC CCTTTTTTCT TTAGCAAACG TGATCTCCAT GGCCATGAGC 
ATGGCCCAGT CTTTCATCCC TCCCACTGGC CTGCCCACCC AGGGGATGGT CACCTATCCA 
GGCTTTCACC CACAGATGTC TGGTCCCATT CCCCCACAGA CTGGTTACCC CTCCGTCTAC 
CCACCTCATT ACCAGAGCCC ACCAGGAGAG TTCTCTTACC CCTCAGTAGG CATCCCAGCA 
CAGAACAATG TCTACCACAG GCCTGAGCCT CAGATGGAAA CTTTGCCCCA GTCCAGTAAC 
CTCAACTGGC AGCATCCTGT CTCACCCCCC TCAATGGCTT CCACCCCTCC CCTGGTTCCC 
CAGGAGCAGC AGGCGGGATT TTCCAATTCC CCCAGACTCA CCCAGGAGGA CCATCTCCAT 
GGATATGCGC CCCCACATAC AGCGCAGGTC CAAACCCAGA CTGAACTCAG ATCAGATGTT 
TCCAGCCCAA ACTCTTTATC AGATCTTTCT CCAGAGAGCA CAAAAAGCAC CTTGTCACCC 
TTTGCCAGTC AGCTCCTCCC CAGTGGAGCT GAGATGACCA CCAGTTCCCC CAAACCAAAA 
ACACCTTGTA AGGAATCTGG AGTCCAGCCT GCACCAGTCA CTGTTGGGAG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCT GGCGCCTCCT GCCCCTGTCG TGTCCGCAGC GGCTGCAGAG 
AGCAGTGGCA CCCCATCAGA GAGCAGCACA GAGGAGCAGG TCGAGTCCGA GACCAGCCTG 
GCCACCTCCC TCACCATGTC CCCTCCTCAT CCACACCCCC ACAGAGGGCC TGCCGGACAC 
CTCCAGGAGG TCGACCCACA GTCTGGATGG GCTAGCAGCC CAGAGCAGGA ACAGGCCCGA 
GTGGAAGAGG AGGAAGATGA GGAGGAGGAG GTGGAAGAGG AAGAGGAGGA GGAAGTGGGG 
GACCGACAGA GGCTGAGGAA GAGAAATCGC AGGAGGGCCT ACAGCCTGAG TTTGCTGGGG 
ACGTCGGCAG ACAGTGGGCT CTCTGTGACG GCCGCTGAGC TGGAGGGTCG ACTGTGGGAT 
GGAGCGGCCG GCAGCCCCCA GTACAGCAAC GCCCTTCACC ATTTATGGAT GATGACCTAC 
AGTCGCAGCA CTCCGTACCT GAGCAGCGAT GAATCCGACA GCGATGACGA GGAAATACTG 
GAGGAGCTTC AGGAGCTCAG AGAGAAACAT CTGACGGAGG TCCAGACCCT GCAGGCTGCC 
CAGAAGAGAG AGATCGAGGA GTTGTACGAG AAGATGGGGA AGGTCCCTCC GGCGGGTATC 
GTCTCTCCCG CCGCCGTGCT GTCCAGGCGG CAGCGTCGCC TGTCAAAGGG TGGCGGCTTC 
CCCTCCTCTC GCAGGAACAG CCTGCAGCGC TTGGACATCC TTCCGCTCCA GGGTATCATC 
AGGAGAAACT CTTTAGGTGG CAGCAGCAGC GGCTCCCAGG ATAAACCAAG CAAAGGCGTC 
ACCTTTTCCA GCGATATCAG AATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615186.1
CDS	204..4670
Translation	MSHAQLEIVGRGFPGHPMDMTDDCLSPVPSVLRAHGLGARRNSYGLHKISMDIDSPLYSGTLSKALSWSAENILTLSESRVEEEKNDYSPPSPSPVSSPATGSITPPCSQEPDAAAQGSSAQSSESENEVQNKQQKILSRVKFDAQWEQDEKEDVETKAVATSPDGRYLKFNIEIGRGSFKTVYKGLDTETTVEVAWCELQTRKLTKAERQRFSEEVEMLKCLQHPNIVRFHDSWKSTVKGLKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIGGCIRMNNDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKPGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKMVAKAIRDRVTAIKRQREKMRKQAEEHKKKQQQEAIEEEPEPPPVLPKVFDAVPVESPPQALTPLAPILSPVTSSVDSGVSSSYPAEPEEPEADQHFHMRHNSLSSANSDCETDGYLSSSGLQDPLEAASFNSQATPPATYNEGAPTGNGFPIAALRFPSSIAVSNSIENGNSRSPSGLNSPVDSYASDVTSGLSDGYEGLSEKSEKTAARKTAGKMFRRRARSRLRITGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIATVMVHNEFILPSEKEGFIHRMGDIIKRAESLMAKGQPGHASVHRLTQPAFHSGANSLSSSQPNLHSHNRPRTSSSSSLPDFSVANPRAGLGGPSGDIFGADITSPERPLIRSQSFHNSPGSPLHHHHHYHPAPALPHHQHYHLPYSNFPFPYPGSPGSPKPAHHPPLTRVASNPTFPSVHSPAPGSPNSLNEAPHPGSNDSITLSPPVTQHPNMWPPHTQPLFSLANVISMAMSMAQSFIPPTGLPTQGMVTYPGFHPQMSGPIPPQTGYPSVYPPHYQSPPGEFSYPSVGIPAQNNVYHRPEPQMETLPQSSNLNWQHPVSPPSMASTPPLVPQEQQAGFSNSPRLTQEDHLHGYAPPHTAQVQTQTELRSDVSSPNSLSDLSPESTKSTLSPFASQLLPSGAEMTTSSPKPKTPCKESGVQPAPVTVGRFQVTPSMDILAPPAPVVSAAAAESSGTPSESSTEEQVESETSLATSLTMSPPHPHPHRGPAGHLQEVDPQSGWASSPEQEQARVEEEEDEEEEVEEEEEEEVGDRQRLRKRNRRRAYSLSLLGTSADSGLSVTAAELEGRLWDGAAGSPQYSNALHHLWMMTYSRSTPYLSSDESDSDDEEILEELQELREKHLTEVQTLQAAQKREIEELYEKMGKVPPAGIVSPAAVLSRRQRRLSKGGGFPSSRRNSLQRLDILPLQGIIRRNSLGGSSSGSQDKPSKGVTFSSDIRM

Target ORF information:

RefSeq Version	XM_011616884.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011616884.1

ORF Insert Sequence:

ATGTCACACG CACAGCTTGA GATAGTAGGA CGCGGCTTCC CGGGACACCC TATGGACATG 
ACCGACGACT GCTTATCCCC CGTCCCATCC GTGCTGAGGG CGCACGGTTT GGGCGCACGG 
AGGAACTCGT ACGGTCTGCA TAAAATCAGC ATGGACATCG ATTCTCCTCT GTACAGCGGC 
ACACTTTCCA AAGCCTTGTC TTGGTCTGCT GAGAACATTC TGACACTGTC CGAGTCCAGA 
GTAGAGGAGG AAAAAAACGA CTACTCCCCG CCGTCTCCGT CGCCGGTGTC CAGCCCGGCC 
ACCGGCTCCA TCACGCCCCC CTGCAGCCAG GAGCCAGACG CCGCCGCGCA GGGATCCAGC 
GCACAGAGTT CGGAGTCTGA AAACGAAGTT CAGAATAAGC AGCAAAAGAT CCTCTCTCGC 
GTCAAGTTCG ATGCTCAGTG GGAGCAGGAC GAGAAAGAAG ACGTCGAGAC CAAAGCGGTG 
GCGACTTCGC CCGATGGAAG ATACCTCAAA TTCAACATTG AGATCGGGAG GGGCTCGTTC 
AAGACGGTCT ATAAGGGACT GGACACTGAA ACGACGGTGG AAGTGGCGTG GTGTGAGTTA 
CAGACTCGGA AGCTGACCAA GGCGGAGCGG CAGCGTTTCA GCGAGGAGGT GGAAATGCTG 
AAGTGCCTGC AGCACCCCAA CATCGTCCGC TTCCACGACT CCTGGAAGTC GACGGTGAAG 
GGCCTCAAAT GCATCATCCT GGTGACGGAA CTGATGACGT CGGGCACGCT CAAAACGTAC 
TTGAAGAGAT TCAAGGAGAT GAAGCTAAAG CTGCTCCAGC GTTGGAGTCG ACAAATCCTC 
AAAGGGCTTC ACTTCCTGCA CACGCGAACG CCTCCCATCA TCCACAGAGA CCTGAAGTGT 
GACAACATCT TCATCACGGG CCCCACTGGC TCTGTCAAGA TTGGAGATCT GGGACTTGCC 
ACTCTTAAAA GTGCCTCTTT TGCTAAAAGT GTCATTGGAA CGCCAGAGTT CATGGCCCCG 
GAGATGTACG AGGAGAAGTA CGACGAAGCG GTGGACGTCT ACGCCTTCGG AATGTGCATC 
CTGGAGATGG CCACCTCTGA GTATCCGTAC TCCGAGTGCC AAAACGCCGC CCAGATCTAC 
CGCAAAGTCA CCAGTGGAAT TAAACCAGAC AGTTTCTACA AAGTCAAAGT CCCGGAGCTC 
AAAGAGATCA TCGGGGGCTG CATTCGCATG AACAACGATG AAAGGTACAC CATTCAGGAC 
CTCTTGGATC ACCCCTTCTT CCAGGAGAAC AACGGCGTGC ACGTGGAGCT GGCGGAGGAG 
GACGACATGG TGAAGCCAGG CCTGAAGCTG TGGCTGCGCA TGGACGACAC AAAGAAGCTC 
CACGGGAAGT ACAAGGACAA CAACGCCATC GAGTTCCTCT TTGAGCTCTA CAAAGACGTG 
CCCGAGGAGG TGGCTCAGGA GATGGTGGTG CTCGGGTTCG TGTGCGAAGC CGACTTCAAA 
ATGGTGGCCA AGGCCATACG GGACAGGGTG ACAGCCATCA AGCGGCAAAG GGAGAAGATG 
AGGAAGCAGG CGGAGGAGCA TAAGAAGAAG CAGCAGCAGG AAGCCATAGA AGAAGAGCCG 
GAACCTCCGC CTGTTTTGCC CAAAGTTTTC GACGCCGTCC CGGTGGAGTC TCCACCCCAA 
GCCCTAACCC CACTGGCTCC CATCCTGTCC CCGGTCACCA GCTCCGTGGA CTCTGGCGTC 
AGCTCCAGCT ACCCCGCAGA GCCGGAGGAG CCTGAGGCCG ACCAGCACTT CCACATGCGC 
CACAACAGCC TGTCATCTGC TAACTCTGAC TGCGAGACAG ACGGCTATCT GAGCTCCTCT 
GGTCTTCAGG ATCCACTGGA GGCCGCCAGC TTTAACTCGC AGGCCACACC TCCTGCCACA 
TACAACGAAG GCGCACCAAC TGGAAACGGT TTCCCCATCG CGGCTCTGCG CTTCCCCTCA 
AGCATCGCTG TATCCAACAG CATAGAGAAC GGAAACTCGA GATCTCCGAG TGGCCTCAAC 
TCACCTGTGG ACAGTTACGC CTCTGACGTG ACCTCGGGTT TGAGTGATGG CTACGAGGGC 
CTATCGGAGA AGAGCGAGAA AACGGCCGCC AGGAAAACTG CTGGGAAGAT GTTCAGAAGG 
AGGGCGCGAT CAAGGCTGCG CATCACAGGG CTCTCTGATA AAGTGGACCG GGTGGTGGAG 
TGCCAGCTGC AGACGCACAA CGACAAGATG GTGACGTTCA AGTTCGACCT GGACGGAGAC 
AATCCCGAAG ACATCGCCAC TGTCATGGTG CACAATGAGT TCATCCTGCC GTCGGAGAAA 
GAGGGCTTCA TCCACCGTAT GGGGGACATC ATCAAGCGTG CAGAGTCTCT GATGGCCAAA 
GGGCAGCCAG GCCACGCCAG CGTGCACCGC CTCACACAGC CCGCTTTCCA CAGTGGGGCT 
AATTCACTGT CTTCGTCACA GCCTAATCTG CACAGCCACA ACCGCCCTCG CACCAGCTCC 
TCCTCATCTC TACCTGACTT TAGCGTGGCT AACCCAAGAG CTGGGCTGGG TGGCCCCAGT 
GGCGACATCT TCGGTGCAGA CATTACTTCA CCTGAGCGAC CTTTAATACG CTCACAGTCT 
TTCCACAACA GCCCAGGTTC TCCTCTCCAT CATCACCACC ACTACCATCC TGCACCTGCC 
CTGCCGCACC ACCAGCATTA CCACTTGCCT TACAGTAACT TCCCATTCCC GTACCCCGGC 
TCTCCAGGCT CCCCCAAGCC GGCCCACCAC CCACCGCTCA CACGTGTAGC CAGTAACCCC 
ACCTTCCCTT CTGTTCACTC CCCTGCCCCC GGTTCGCCCA ATTCCCTCAA CGAGGCCCCG 
CATCCAGGTA GCAACGACAG TATCACCCTG TCCCCACCTG TGACTCAGCA TCCCAACATG 
TGGCCCCCCC ACACGCAGCC CCTTTTTTCT TTAGCAAACG TGATCTCCAT GGCCATGAGC 
ATGGCCCAGT CTTTCATCCC TCCCACTGGC CTGCCCACCC AGGGGATGGT CACCTATCCA 
GGCTTTCACC CACAGATGTC TGGTCCCATT CCCCCACAGA CTGGTTACCC CTCCGTCTAC 
CCACCTCATT ACCAGAGCCC ACCAGGAGAG TTCTCTTACC CCTCAGTAGG CATCCCAGCA 
CAGAACAATG TCTACCACAG GCCTGAGCCT CAGATGGAAA CTTTGCCCCA GTCCAGTAAC 
CTCAACTGGC AGCATCCTGT CTCACCCCCC TCAATGGCTT CCACCCCTCC CCTGGTTCCC 
CAGGAGCAGC AGGCGGGATT TTCCAATTCC CCCAGACTCA CCCAGGAGGA CCATCTCCAT 
GGATATGCGC CCCCACATAC AGCGCAGGTC CAAACCCAGA CTGAACTCAG ATCAGATGTT 
TCCAGCCCAA ACTCTTTATC AGATCTTTCT CCAGAGAGCA CAAAAAGCAC CTTGTCACCC 
TTTGCCAGTC AGCTCCTCCC CAGTGGAGCT GAGATGACCA CCAGTTCCCC CAAACCAAAA 
ACACCTTGTA AGGAATCTGG AGTCCAGCCT GCACCAGTCA CTGTTGGGAG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCT GGCGCCTCCT GCCCCTGTCG TGTCCGCAGC GGCTGCAGAG 
AGCAGTGGCA CCCCATCAGA GAGCAGCACA GAGGAGCAGG TCGAGTCCGA GACCAGCCTG 
GCCACCTCCC TCACCATGTC CCCTCCTCAT CCACACCCCC ACAGAGGGCC TGCCGGACAC 
CTCCAGGAGG TCGACCCACA GTCTGGATGG GCTAGCAGCC CAGAGCAGGA ACAGGCCCGA 
GTGGAAGAGG AGGAAGATGA GGAGGAGGAG GTGGAAGAGG AAGAGGAGGA GGAAGTGGGG 
GACCGACAGA GGCTGAGGAA GAGAAATCGC AGGAGGGCCT ACAGCCTGAG TTTGCTGGGG 
ACGTCGGCAG ACAGTGGGCT CTCTGTGACG GCCGCTGAGC TGGAGGGTCG ACTGTGGGAT 
GGAGCGGCCG GCAGCCCCCA GTACAGCAAC GCCCTTCACC ATTTATGGAT GATGACCTAC 
AGTCGCAGCA CTCCGTACCT GAGCAGCGAT GAATCCGACA GCGATGACGA GGAAATACTG 
GAGGAGCTTC AGGAGCTCAG AGAGAAACAT CTGACGGAGG TCCAGACCCT GCAGGCTGCC 
CAGAAGAGAG AGATCGAGGA GTTGTACGAG AAGATGGGGA AGGTCCCTCC GGCGGGTATC 
GTCTCTCCCG CCGCCGTGCT GTCCAGGCGG CAGCGTCGCC TGTCAAAGGG TGGCGGCTTC 
CCCTCCTCTC GCAGGAACAG CCTGCAGCGC TTGGACATCC TTCCGCTCCA GGGTATCATC 
AGGAGAAACT CTTTAGGTGG CAGCAGCAGC GGCTCCCAGG ATAAACCAAG CAAAGGCGTC 
ACCTTTTCCA GCGATATCAG AATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk4 ( XM_011617375.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011615677.1 Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo22776
Clone ID Related Accession (Same CDS sequence)	XM_011617375.1
Accession Version	XM_011617375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	serine/threonine-protein kinase WNK4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070896.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACACG CACAGCTTGA GATAGTAGGA CGCGGCTTCC CGGGACACCC TATGGACATG 
ACCGACGACT GCTTATCCCC CGTCCCATCC GTGCTGAGGG CGCACGGTTT GGGCGCACGG 
AGGAACTCGT ACGGTCTGCA TAAAATCAGC ATGGACATCG ATTCTCCTCT GTACAGCGGC 
ACACTTTCCA AAGCCTTGTC TTGGTCTGCT GAGAACATTC TGACACTGTC CGAGTCCAGA 
GTAGAGGAGG AAAAAAACGA CTACTCCCCG CCGTCTCCGT CGCCGGTGTC CAGCCCGGCC 
ACCGGCTCCA TCACGCCCCC CTGCAGCCAG GAGCCAGACG CCGCCGCGCA GGGATCCAGC 
GCACAGAGTT CGGAGTCTGA AAACGAAGTT CAGAATAAGC AGCAAAAGAT CCTCTCTCGC 
GTCAAGTTCG ATGCTCAGTG GGAGCAGGAC GAGAAAGAAG ACGTCGAGAC CAAAGCGGTG 
GCGACTTCGC CCGATGGAAG ATACCTCAAA TTCAACATTG AGATCGGGAG GGGCTCGTTC 
AAGACGGTCT ATAAGGGACT GGACACTGAA ACGACGGTGG AAGTGGCGTG GTGTGAGTTA 
CAGACTCGGA AGCTGACCAA GGCGGAGCGG CAGCGTTTCA GCGAGGAGGT GGAAATGCTG 
AAGTGCCTGC AGCACCCCAA CATCGTCCGC TTCCACGACT CCTGGAAGTC GACGGTGAAG 
GGCCTCAAAT GCATCATCCT GGTGACGGAA CTGATGACGT CGGGCACGCT CAAAACGTAC 
TTGAAGAGAT TCAAGGAGAT GAAGCTAAAG CTGCTCCAGC GTTGGAGTCG ACAAATCCTC 
AAAGGGCTTC ACTTCCTGCA CACGCGAACG CCTCCCATCA TCCACAGAGA CCTGAAGTGT 
GACAACATCT TCATCACGGG CCCCACTGGC TCTGTCAAGA TTGGAGATCT GGGACTTGCC 
ACTCTTAAAA GTGCCTCTTT TGCTAAAAGT GTCATTGGAA CGCCAGAGTT CATGGCCCCG 
GAGATGTACG AGGAGAAGTA CGACGAAGCG GTGGACGTCT ACGCCTTCGG AATGTGCATC 
CTGGAGATGG CCACCTCTGA GTATCCGTAC TCCGAGTGCC AAAACGCCGC CCAGATCTAC 
CGCAAAGTCA CCAGTGGAAT TAAACCAGAC AGTTTCTACA AAGTCAAAGT CCCGGAGCTC 
AAAGAGATCA TCGGGGGCTG CATTCGCATG AACAACGATG AAAGGTACAC CATTCAGGAC 
CTCTTGGATC ACCCCTTCTT CCAGGAGAAC AACGGCGTGC ACGTGGAGCT GGCGGAGGAG 
GACGACATGG TGAAGCCAGG CCTGAAGCTG TGGCTGCGCA TGGACGACAC AAAGAAGCTC 
CACGGGAAGT ACAAGGACAA CAACGCCATC GAGTTCCTCT TTGAGCTCTA CAAAGACGTG 
CCCGAGGAGG TGGCTCAGGA GATGGTGGTG CTCGGGTTCG TGTGCGAAGC CGACTTCAAA 
ATGGTGGCCA AGGCCATACG GGACAGGGTG ACAGCCATCA AGCGGCAAAG GGAGAAGATG 
AGGAAGCAGG CGGAGGAGCA TAAGAAGAAG CAGCAGCAGG AAGCCATAGA AGAAGAGCCG 
GAACCTCCGC CTGTTTTGCC CAAAGTTTTC GACGCCGTCC CGGTGGAGTC TCCACCCCAA 
GCCCTAACCC CACTGGCTCC CATCCTGTCC CCGGTCACCA GCTCCGTGGA CTCTGGCGTC 
AGCTCCAGCT ACCCCGCAGA GCCGGAGGAG CCTGAGGCCG ACCAGCACTT CCACATGCGC 
CACAACAGCC TGTCATCTGC TAACTCTGAC TGCGAGACAG ACGGCTATCT GAGCTCCTCT 
GGTCTTCAGG ATCCACTGGA GGCCGCCAGC TTTAACTCGC AGGCCACACC TCCTGCCACA 
TACAACGAAG GCGCACCAAC TGGAAACGGT TTCCCCATCG CGGCTCTGCG CTTCCCCTCA 
AGCATCGCTG TATCCAACAG CATAGAGAAC GGAAACTCGA GATCTCCGAG TGGCCTCAAC 
TCACCTGTGG ACAGTTACGC CTCTGACGTG ACCTCGGGTT TGAGTGATGG CTACGAGGGC 
CTATCGGAGA AGAGCGAGAA AACGGCCGCC AGGAAAACTG CTGGGAAGAT GTTCAGAAGG 
AGGGCGCGAT CAAGGCTGCG CATCACAGGG CTCTCTGATA AAGTGGACCG GGTGGTGGAG 
TGCCAGCTGC AGACGCACAA CGACAAGATG GTGACGTTCA AGTTCGACCT GGACGGAGAC 
AATCCCGAAG ACATCGCCAC TGTCATGGTG CACAATGAGT TCATCCTGCC GTCGGAGAAA 
GAGGGCTTCA TCCACCGTAT GGGGGACATC ATCAAGCGTG CAGAGTCTCT GATGGCCAAA 
GGGCAGCCAG GCCACGCCAG CGTGCACCGC CTCACACAGC CCGCTTTCCA CAGTGGGGCT 
AATTCACTGT CTTCGTCACA GCCTAATCTG CACAGCCACA ACCGCCCTCG CACCAGCTCC 
TCCTCATCTC TACCTGGTTC TCCTCTCCAT CATCACCACC ACTACCATCC TGCACCTGCC 
CTGCCGCACC ACCAGCATTA CCACTTGCCT TACAGTAACT TCCCATTCCC GTACCCCGGC 
TCTCCAGGCT CCCCCAAGCC GGCCCACCAC CCACCGCTCA CACGTGTAGC CAGTAACCCC 
ACCTTCCCTT CTGTTCACTC CCCTGCCCCC GGTTCGCCCA ATTCCCTCAA CGAGGCCCCG 
CATCCAGGTA GCAACGACAG TATCACCCTG TCCCCACCTG TGACTCAGCA TCCCAACATG 
TGGCCCCCCC ACACGCAGCC CCTTTTTTCT TTAGCAAACG TGATCTCCAT GGCCATGAGC 
ATGGCCCAGT CTTTCATCCC TCCCACTGGC CTGCCCACCC AGGGGATGGT CACCTATCCA 
GGCTTTCACC CACAGATGTC TGGTCCCATT CCCCCACAGA CTGGTTACCC CTCCGTCTAC 
CCACCTCATT ACCAGAGCCC ACCAGGAGAG TTCTCTTACC CCTCAGTAGG CATCCCAGCA 
CAGAACAATG TCTACCACAG GCCTGAGCCT CAGATGGAAA CTTTGCCCCA GTCCAGTAAC 
CTCAACTGGC AGCATCCTGT CTCACCCCCC TCAATGGCTT CCACCCCTCC CCTGGTTCCC 
CAGGAGCAGC AGGCGGGATT TTCCAATTCC CCCAGACTCA CCCAGGAGGA CCATCTCCAT 
GGATATGCGC CCCCACATAC AGCGCAGGTC CAAACCCAGA CTGAACTCAG ATCAGATGTT 
TCCAGCCCAA ACTCTTTATC AGATCTTTCT CCAGAGAGCA CAAAAAGCAC CTTGTCACCC 
TTTGCCAGTC AGCTCCTCCC CAGTGGAGCT GAGATGACCA CCAGTTCCCC CAAACCAAAA 
ACACCTTGTA AGGAATCTGG AGTCCAGCCT GCACCAGTCA CTGTTGGGAG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCT GGCGCCTCCT GCCCCTGTCG TGTCCGCAGC GGCTGCAGAG 
AGCAGTGGCA CCCCATCAGA GAGCAGCACA GAGGAGCAGG TCGAGTCCGA GACCAGCCTG 
GCCACCTCCC TCACCATGTC CCCTCCTCAT CCACACCCCC ACAGAGGGCC TGCCGGACAC 
CTCCAGGAGG TCGACCCACA GTCTGGATGG GCTAGCAGCC CAGAGCAGGA ACAGGCCCGA 
GTGGAAGAGG AGGAAGATGA GGAGGAGGAG GTGGAAGAGG AAGAGGAGGA GGAAGTGGGG 
GACCGACAGA GGCTGAGGAA GAGAAATCGC AGGAGGGCCT ACAGCCTGAG TTTGCTGGGG 
ACGTCGGCAG ACAGTGGGCT CTCTGTGACG GCCGCTGAGC TGGAGGGTCG ACTGTGGGAT 
GGAGCGGCCG GCAGCCCCCA GTACAGCAAC GCCCTTCACC ATTTATGGAT GATGACCTAC 
AGTCGCAGCA CTCCGTACCT GAGCAGCGAT GAATCCGACA GCGATGACGA GGAAATACTG 
GAGGAGCTTC AGGAGCTCAG AGAGAAACAT CTGACGGAGG TCCAGACCCT GCAGGCTGCC 
CAGAAGAGAG AGATCGAGGA GTTGTACGAG AAGATGGGGA AGGTCCCTCC GGCGGGTATC 
GTCTCTCCCG CCGCCGTGCT GTCCAGGCGG CAGCGTCGCC TGTCAAAGGG TGGCGGCTTC 
CCCTCCTCTC GCAGGAACAG CCTGCAGCGC TTGGACATCC TTCCGCTCCA GGGTATCATC 
AGGAGAAACT CTTTAGGTGG CAGCAGCAGC GGCTCCCAGG ATAAACCAAG CAAAGGCGTC 
ACCTTTTCCA GCGATATCAG AATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615677.1
CDS	204..4550
Translation	MSHAQLEIVGRGFPGHPMDMTDDCLSPVPSVLRAHGLGARRNSYGLHKISMDIDSPLYSGTLSKALSWSAENILTLSESRVEEEKNDYSPPSPSPVSSPATGSITPPCSQEPDAAAQGSSAQSSESENEVQNKQQKILSRVKFDAQWEQDEKEDVETKAVATSPDGRYLKFNIEIGRGSFKTVYKGLDTETTVEVAWCELQTRKLTKAERQRFSEEVEMLKCLQHPNIVRFHDSWKSTVKGLKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIGGCIRMNNDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKPGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKMVAKAIRDRVTAIKRQREKMRKQAEEHKKKQQQEAIEEEPEPPPVLPKVFDAVPVESPPQALTPLAPILSPVTSSVDSGVSSSYPAEPEEPEADQHFHMRHNSLSSANSDCETDGYLSSSGLQDPLEAASFNSQATPPATYNEGAPTGNGFPIAALRFPSSIAVSNSIENGNSRSPSGLNSPVDSYASDVTSGLSDGYEGLSEKSEKTAARKTAGKMFRRRARSRLRITGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIATVMVHNEFILPSEKEGFIHRMGDIIKRAESLMAKGQPGHASVHRLTQPAFHSGANSLSSSQPNLHSHNRPRTSSSSSLPGSPLHHHHHYHPAPALPHHQHYHLPYSNFPFPYPGSPGSPKPAHHPPLTRVASNPTFPSVHSPAPGSPNSLNEAPHPGSNDSITLSPPVTQHPNMWPPHTQPLFSLANVISMAMSMAQSFIPPTGLPTQGMVTYPGFHPQMSGPIPPQTGYPSVYPPHYQSPPGEFSYPSVGIPAQNNVYHRPEPQMETLPQSSNLNWQHPVSPPSMASTPPLVPQEQQAGFSNSPRLTQEDHLHGYAPPHTAQVQTQTELRSDVSSPNSLSDLSPESTKSTLSPFASQLLPSGAEMTTSSPKPKTPCKESGVQPAPVTVGRFQVTPSMDILAPPAPVVSAAAAESSGTPSESSTEEQVESETSLATSLTMSPPHPHPHRGPAGHLQEVDPQSGWASSPEQEQARVEEEEDEEEEVEEEEEEEVGDRQRLRKRNRRRAYSLSLLGTSADSGLSVTAAELEGRLWDGAAGSPQYSNALHHLWMMTYSRSTPYLSSDESDSDDEEILEELQELREKHLTEVQTLQAAQKREIEELYEKMGKVPPAGIVSPAAVLSRRQRRLSKGGGFPSSRRNSLQRLDILPLQGIIRRNSLGGSSSGSQDKPSKGVTFSSDIRM

Target ORF information:

RefSeq Version	XM_011617375.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617375.1

ORF Insert Sequence:

ATGTCACACG CACAGCTTGA GATAGTAGGA CGCGGCTTCC CGGGACACCC TATGGACATG 
ACCGACGACT GCTTATCCCC CGTCCCATCC GTGCTGAGGG CGCACGGTTT GGGCGCACGG 
AGGAACTCGT ACGGTCTGCA TAAAATCAGC ATGGACATCG ATTCTCCTCT GTACAGCGGC 
ACACTTTCCA AAGCCTTGTC TTGGTCTGCT GAGAACATTC TGACACTGTC CGAGTCCAGA 
GTAGAGGAGG AAAAAAACGA CTACTCCCCG CCGTCTCCGT CGCCGGTGTC CAGCCCGGCC 
ACCGGCTCCA TCACGCCCCC CTGCAGCCAG GAGCCAGACG CCGCCGCGCA GGGATCCAGC 
GCACAGAGTT CGGAGTCTGA AAACGAAGTT CAGAATAAGC AGCAAAAGAT CCTCTCTCGC 
GTCAAGTTCG ATGCTCAGTG GGAGCAGGAC GAGAAAGAAG ACGTCGAGAC CAAAGCGGTG 
GCGACTTCGC CCGATGGAAG ATACCTCAAA TTCAACATTG AGATCGGGAG GGGCTCGTTC 
AAGACGGTCT ATAAGGGACT GGACACTGAA ACGACGGTGG AAGTGGCGTG GTGTGAGTTA 
CAGACTCGGA AGCTGACCAA GGCGGAGCGG CAGCGTTTCA GCGAGGAGGT GGAAATGCTG 
AAGTGCCTGC AGCACCCCAA CATCGTCCGC TTCCACGACT CCTGGAAGTC GACGGTGAAG 
GGCCTCAAAT GCATCATCCT GGTGACGGAA CTGATGACGT CGGGCACGCT CAAAACGTAC 
TTGAAGAGAT TCAAGGAGAT GAAGCTAAAG CTGCTCCAGC GTTGGAGTCG ACAAATCCTC 
AAAGGGCTTC ACTTCCTGCA CACGCGAACG CCTCCCATCA TCCACAGAGA CCTGAAGTGT 
GACAACATCT TCATCACGGG CCCCACTGGC TCTGTCAAGA TTGGAGATCT GGGACTTGCC 
ACTCTTAAAA GTGCCTCTTT TGCTAAAAGT GTCATTGGAA CGCCAGAGTT CATGGCCCCG 
GAGATGTACG AGGAGAAGTA CGACGAAGCG GTGGACGTCT ACGCCTTCGG AATGTGCATC 
CTGGAGATGG CCACCTCTGA GTATCCGTAC TCCGAGTGCC AAAACGCCGC CCAGATCTAC 
CGCAAAGTCA CCAGTGGAAT TAAACCAGAC AGTTTCTACA AAGTCAAAGT CCCGGAGCTC 
AAAGAGATCA TCGGGGGCTG CATTCGCATG AACAACGATG AAAGGTACAC CATTCAGGAC 
CTCTTGGATC ACCCCTTCTT CCAGGAGAAC AACGGCGTGC ACGTGGAGCT GGCGGAGGAG 
GACGACATGG TGAAGCCAGG CCTGAAGCTG TGGCTGCGCA TGGACGACAC AAAGAAGCTC 
CACGGGAAGT ACAAGGACAA CAACGCCATC GAGTTCCTCT TTGAGCTCTA CAAAGACGTG 
CCCGAGGAGG TGGCTCAGGA GATGGTGGTG CTCGGGTTCG TGTGCGAAGC CGACTTCAAA 
ATGGTGGCCA AGGCCATACG GGACAGGGTG ACAGCCATCA AGCGGCAAAG GGAGAAGATG 
AGGAAGCAGG CGGAGGAGCA TAAGAAGAAG CAGCAGCAGG AAGCCATAGA AGAAGAGCCG 
GAACCTCCGC CTGTTTTGCC CAAAGTTTTC GACGCCGTCC CGGTGGAGTC TCCACCCCAA 
GCCCTAACCC CACTGGCTCC CATCCTGTCC CCGGTCACCA GCTCCGTGGA CTCTGGCGTC 
AGCTCCAGCT ACCCCGCAGA GCCGGAGGAG CCTGAGGCCG ACCAGCACTT CCACATGCGC 
CACAACAGCC TGTCATCTGC TAACTCTGAC TGCGAGACAG ACGGCTATCT GAGCTCCTCT 
GGTCTTCAGG ATCCACTGGA GGCCGCCAGC TTTAACTCGC AGGCCACACC TCCTGCCACA 
TACAACGAAG GCGCACCAAC TGGAAACGGT TTCCCCATCG CGGCTCTGCG CTTCCCCTCA 
AGCATCGCTG TATCCAACAG CATAGAGAAC GGAAACTCGA GATCTCCGAG TGGCCTCAAC 
TCACCTGTGG ACAGTTACGC CTCTGACGTG ACCTCGGGTT TGAGTGATGG CTACGAGGGC 
CTATCGGAGA AGAGCGAGAA AACGGCCGCC AGGAAAACTG CTGGGAAGAT GTTCAGAAGG 
AGGGCGCGAT CAAGGCTGCG CATCACAGGG CTCTCTGATA AAGTGGACCG GGTGGTGGAG 
TGCCAGCTGC AGACGCACAA CGACAAGATG GTGACGTTCA AGTTCGACCT GGACGGAGAC 
AATCCCGAAG ACATCGCCAC TGTCATGGTG CACAATGAGT TCATCCTGCC GTCGGAGAAA 
GAGGGCTTCA TCCACCGTAT GGGGGACATC ATCAAGCGTG CAGAGTCTCT GATGGCCAAA 
GGGCAGCCAG GCCACGCCAG CGTGCACCGC CTCACACAGC CCGCTTTCCA CAGTGGGGCT 
AATTCACTGT CTTCGTCACA GCCTAATCTG CACAGCCACA ACCGCCCTCG CACCAGCTCC 
TCCTCATCTC TACCTGGTTC TCCTCTCCAT CATCACCACC ACTACCATCC TGCACCTGCC 
CTGCCGCACC ACCAGCATTA CCACTTGCCT TACAGTAACT TCCCATTCCC GTACCCCGGC 
TCTCCAGGCT CCCCCAAGCC GGCCCACCAC CCACCGCTCA CACGTGTAGC CAGTAACCCC 
ACCTTCCCTT CTGTTCACTC CCCTGCCCCC GGTTCGCCCA ATTCCCTCAA CGAGGCCCCG 
CATCCAGGTA GCAACGACAG TATCACCCTG TCCCCACCTG TGACTCAGCA TCCCAACATG 
TGGCCCCCCC ACACGCAGCC CCTTTTTTCT TTAGCAAACG TGATCTCCAT GGCCATGAGC 
ATGGCCCAGT CTTTCATCCC TCCCACTGGC CTGCCCACCC AGGGGATGGT CACCTATCCA 
GGCTTTCACC CACAGATGTC TGGTCCCATT CCCCCACAGA CTGGTTACCC CTCCGTCTAC 
CCACCTCATT ACCAGAGCCC ACCAGGAGAG TTCTCTTACC CCTCAGTAGG CATCCCAGCA 
CAGAACAATG TCTACCACAG GCCTGAGCCT CAGATGGAAA CTTTGCCCCA GTCCAGTAAC 
CTCAACTGGC AGCATCCTGT CTCACCCCCC TCAATGGCTT CCACCCCTCC CCTGGTTCCC 
CAGGAGCAGC AGGCGGGATT TTCCAATTCC CCCAGACTCA CCCAGGAGGA CCATCTCCAT 
GGATATGCGC CCCCACATAC AGCGCAGGTC CAAACCCAGA CTGAACTCAG ATCAGATGTT 
TCCAGCCCAA ACTCTTTATC AGATCTTTCT CCAGAGAGCA CAAAAAGCAC CTTGTCACCC 
TTTGCCAGTC AGCTCCTCCC CAGTGGAGCT GAGATGACCA CCAGTTCCCC CAAACCAAAA 
ACACCTTGTA AGGAATCTGG AGTCCAGCCT GCACCAGTCA CTGTTGGGAG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCT GGCGCCTCCT GCCCCTGTCG TGTCCGCAGC GGCTGCAGAG 
AGCAGTGGCA CCCCATCAGA GAGCAGCACA GAGGAGCAGG TCGAGTCCGA GACCAGCCTG 
GCCACCTCCC TCACCATGTC CCCTCCTCAT CCACACCCCC ACAGAGGGCC TGCCGGACAC 
CTCCAGGAGG TCGACCCACA GTCTGGATGG GCTAGCAGCC CAGAGCAGGA ACAGGCCCGA 
GTGGAAGAGG AGGAAGATGA GGAGGAGGAG GTGGAAGAGG AAGAGGAGGA GGAAGTGGGG 
GACCGACAGA GGCTGAGGAA GAGAAATCGC AGGAGGGCCT ACAGCCTGAG TTTGCTGGGG 
ACGTCGGCAG ACAGTGGGCT CTCTGTGACG GCCGCTGAGC TGGAGGGTCG ACTGTGGGAT 
GGAGCGGCCG GCAGCCCCCA GTACAGCAAC GCCCTTCACC ATTTATGGAT GATGACCTAC 
AGTCGCAGCA CTCCGTACCT GAGCAGCGAT GAATCCGACA GCGATGACGA GGAAATACTG 
GAGGAGCTTC AGGAGCTCAG AGAGAAACAT CTGACGGAGG TCCAGACCCT GCAGGCTGCC 
CAGAAGAGAG AGATCGAGGA GTTGTACGAG AAGATGGGGA AGGTCCCTCC GGCGGGTATC 
GTCTCTCCCG CCGCCGTGCT GTCCAGGCGG CAGCGTCGCC TGTCAAAGGG TGGCGGCTTC 
CCCTCCTCTC GCAGGAACAG CCTGCAGCGC TTGGACATCC TTCCGCTCCA GGGTATCATC 
AGGAGAAACT CTTTAGGTGG CAGCAGCAGC GGCTCCCAGG ATAAACCAAG CAAAGGCGTC 
ACCTTTTCCA GCGATATCAG AATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk4 ( XM_011616884.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011615186.2 Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X1, mRNA.

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CloneID	OTo43121
Clone ID Related Accession (Same CDS sequence)	XM_011616884.2
Accession Version	XM_011616884.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	serine/threonine-protein kinase WNK4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011616884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACACG CACAGCTTGA GATAGTAGGA CGCGGCTTCC CGGGACACCC TATGGACATG 
ACCGACGACT GCTTATCCCC CGTCCCATCC GTGCTGAGGG CGCACGGTTT GGGCGCACGG 
AGGAACTCGT ACGGTCTGCA TAAAATCAGC ATGGACATCG ATTCTCCTCT GTACAGCGGC 
ACACTTTCCA AAGCCTTGTC TTGGTCTGCT GAGAACATTC TGACACTGTC CGAGTCCAGA 
GTAGAGGAGG AAAAAAACGA CTACTCTCCG CCGTCTCCGT CGCCGGTGTC CAGCCCGGCC 
ACCGGCTCCA TCACGCCCCC CTGCAGCCAG GAGCCAGACG CCGCCGCGCA GGGATCCAGC 
GCACAGAGTT CGGAGTCTGA AAACGAAGTT CAGAATAAGC AGCAAAAGAT CCTCTCTCGC 
GTCAAGTTCG ATGCTCAGTG GGAGCAGGAC GAGAAAGAAG ACGTCGAGAC CAAAGCGGTG 
GCGACTTCGC CCGATGGAAG ATACCTCAAA TTCAACATTG AGATCGGGAG GGGCTCGTTC 
AAGACGGTCT ATAAGGGACT GGACACTGAA ACGACGGTGG AAGTGGCGTG GTGTGAGTTA 
CAGACTCGGA AGCTGACCAA GGCGGAGCGG CAGCGTTTCA GCGAGGAGGT GGAAATGCTG 
AAGTGCCTGC AGCACCCCAA CATCGTCCGC TTCCACGACT CCTGGAAGTC GACGGTGAAG 
GGCCTCAAAT GCATCATCCT GGTGACGGAA CTGATGACGT CGGGCACGCT CAAAACGTAC 
TTGAAGAGAT TCAAGGAGAT GAAGCTAAAG CTGCTCCAGC GTTGGAGTCG ACAAATCCTC 
AAAGGGCTTC ACTTCCTGCA CACGCGAACG CCTCCCATCA TCCACAGAGA CCTGAAGTGT 
GACAACATCT TCATCACGGG CCCCACTGGC TCTGTCAAGA TTGGAGATCT GGGACTTGCC 
ACTCTTAAAA GTGCCTCTTT TGCTAAAAGT GTCATTGGAA CGCCAGAGTT CATGGCCCCG 
GAGATGTACG AGGAGAAGTA CGACGAAGCG GTGGACGTCT ACGCCTTCGG AATGTGCATC 
CTGGAGATGG CCACCTCTGA GTATCCGTAC TCCGAGTGCC AAAACGCCGC CCAGATCTAC 
CGCAAAGTCA CCAGTGGAAT TAAACCAGAC AGTTTCTACA AAGTCAAAGT CCCGGAGCTC 
AAAGAGATCA TCGGGGGCTG CATTCGCATG AACAACGATG AAAGGTACAC CATTCAGGAC 
CTCTTGGATC ACCCCTTCTT CCAGGAGAAC AACGGCGTGC ACGTGGAGCT GGCGGAGGAG 
GACGACATGG TGAAGCCAGG CCTGAAGCTG TGGCTGCGCA TGGACGACAC AAAGAAGCTC 
CACGGGAAGT ACAAGGACAA CAACGCCATC GAGTTCCTCT TTGAGCTCTA CAAAGACGTG 
CCCGAGGAGG TGGCTCAGGA GATGGTGGTG CTCGGGTTCG TGTGCGAAGC CGACTTCAAA 
ATGGTGGCCA AGGCCATACG GGACAGGGTG ACAGCCATCA AGCGGCAAAG GGAGAAGATG 
AGGAAGCAGG CGGAGGAGCA TAAGAAGAAG CAGCAGCAGG AAGCCATAGA AGAAGAGCCG 
GAACCTCCGC CTGTTTTGCC CAAAGTTTTC GACGCCGTCC CGGTGGAGTC TCCACCCCAA 
GCCCTAACCC CGCTGGCTCC CATCCTGTCC CCGGTCACCA GCTCCGTGGA CTCTGGCGTC 
AGCTCCAGCT ACCCCGCAGA GCCGGAGGAG CCTGAGGCCG ACCAGCACTT CCACATGCGC 
CACAACAGCC TGTCATCTGC TAACTCTGAC TGCGAGACAG ACGGCTATCT GAGCTCCTCT 
GGTCTTCAGG ATCCACTGGA GGCCGCCAGC TTTAACTCGC AGGCCACACC TCCTGCCACA 
TACAACGAAG GCGCACCAAC TGGAAACGGT TTCCCCATCG CGGCTCTGCG CTTCCCCTCA 
AGCATCGCTG TATCCAACAG CATAGAGAAC GGAAACTCGA GATCTCCGAG TGGCCTCAAC 
TCACCTGTGG ACAGTTACGC CTCTGACGTG ACCTCGGGTT TGAGTGATGG CTACGAGGGC 
CTATCGGAGA AGAGCGAGAA AACGGCCGCC AGGAAAACTG CTGGGAAGAT GTTCAGAAGG 
AGGGCGCGAT CAAGGCTGCG CATCACAGGG CTCTCTGATA AAGTGGACCG GGTGGTGGAG 
TGCCAGCTGC AGACGCACAA CGACAAGATG GTGACGTTCA AGTTCGACCT GGACGGAGAC 
AATCCCGAAG ACATCGCCAC TGTCATGGTG CACAATGAGT TCATCCTGCC GTCGGAGAAA 
GAGGGCTTCA TCCACCGTAT GGGGGACATC ATCAAGCGTG CAGAGTCTCT GATGGCCAAA 
GGGCAGCCAG GCCACGCCAG CGTGCACCGC CTCACACAGC CCGCTTTCCA CAGTGGGGCT 
AATTCACTGT CTTCGTCACA GCCTAATCTG CACAGCCACA ACCGCCCTCG CACCAGCTCC 
TCCTCATCTC TACCTGACTT TAGCGTGGCT AACCCAAGAG CTGGGCTGGG TGGCCCCAGT 
GGCGACATCT TCGGTGCAGA CATTACTTCA CCTGAGCGAC CTTTAATACG CTCACAGTCT 
TTCCACAACA GCCCAGGTTC TCCTCTCCAT CATCACCACC ACTACCATCC TGCACCTGCC 
CTGCCGCACC ACCAGCATTA CCACTTGCCT TACAGTAACT TCCCATTCCC GTACCCCGGC 
TCTCCAGGCT CCCCCAAGCC GGCCCACCAC CCACCGCTCA CACGTGTAGC CAGTAACCCC 
ACCTTCCCTT CCGTTCACTC CCCTGCCCCC GGTTCGCCCA ATTCCCTCAA TGAGGCCCCG 
CATCCAGGTA GCAACGACAG TATCACCCTG TCCCCACCTG TGACTCAGCA TCCCAACATG 
TGGCCCCCCC ACACGCAGCC CCTTTTTTCT TTAGCAAACG TGATCTCCAT GGCCATGAGC 
ATGGCCCAGT CTTTCATCCC TCCCACTGGC CTGCCCACCC AGGGGATGGT CACCTATCCA 
GGCTTTCACC CACAGATGTC TGGTCCCATT CCCCCACAGA CTGGTTACCC CTCCGTCTAC 
CCACCTCATT ACCAGAGCCC ACCAGGAGAG TTCTCTTACC CCTCAGTAGG CATCCCAGCA 
CAGAACAATG TCTACCACAG GCCTGAGCCT CAGATGGAAA CTTTGCCCCA GTCCAGTAAC 
CTCAACTGGC AGCATCCTGT CTCACCCCCC TCAATGGCTT CCACCCCTCC CCTGGTTCCC 
CAGGAGCAGC AGGCGGGATT TTCCAATTCC CCCAGACTCA CCCAGGAGGA CCATCTCCAT 
GGATATGCGC CCCCACATAC AGCGCAGGTC CAAACCCAGA CTGAACTCAG ATCAGATGTT 
TCCAGCCCAA ACTCTTTATC AGATCTTTCT CCAGAGAGCA CAAAAAGCAC CTTGTCACCC 
TTTGCCAGTC AGCTCCTCCC CAGTGGAGCT GAGATGACCA CCAGTTCCCC CAAACCAAAA 
ACACCTTGTA AGGAATCTGG AGTCCAGCCT GCACCAGTCA CTGTTGGGAG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCT GGCGCCTCCT GCCCCTGTCG TGTCCGCAGC GGCTGCAGAG 
AGCAGTGGCA CCCCATCAGA GAGCAGCACA GAGGAGCAGG TCGAGTCCGA GACCAGCCTG 
GCCACCTCCC TCACCATGTC CCCTCCTCAT CCACACCCCC ACAGAGGGCC TGCCGGACAC 
CTCCAGGAGG TCGACCCACA GTCTGGATGG GCTAGCAGCC CAGAGCAGGA ACAGGCCCGA 
GTGGAAGAGG AGGAAGATGA GGAGGAGGAG GTGGAAGAGG AAGAGGAGGA GGAGGAGGAA 
GTGGGGGACC GACAGAGGCT GAGGAAGAGA AATCGCAGGA GGGCCTACAG CCTGAGTTTG 
CTGGGGACGT CGGCAGACAG TGGGCTCTCT GTGACGGCCG CTGAGCTGGA GGGTCGACTG 
TGGGATGGAG CGGCCGGCAG CCCCCAGTAC AGCAACGCCC TTCACCATTT ATGGATGATG 
ACCTACAGTC GCAGCACTCC GTACCTGAGC AGCGATGAAT CCGACAGCGA TGGCGAGGAA 
ATACTGGAGG AGCTTCAGGA GCTCAGAGAG AAACATCTGA CGGAGGTCCA GACCCTGCAG 
GCTGCCCAGA AGAGAGAGAT CGAGGAGTTG TACGAGAAGA TGGGGAAGGT CCCTCCGGCG 
GGTATCGTCT CTCCCGCCGC CGTGCTGTCC AGACGGCAGC GTCGCCTGTC AAAGGGTGGC 
GGCTTCCCCT CCTCTCGCAG GAACAGCCTG CAGCGCTTGG ACATCCTTCC GCTCCAGGGT 
ATCATCAGGA GAAACTCTTT AGGTGGCAGC AGCAGCGGCT CCCAGGATAA ACCAAGCAAA 
GGCGTCACCT TTTCCAGCGA TATCAGAATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615186.2
CDS	221..4693
Translation	MSHAQLEIVGRGFPGHPMDMTDDCLSPVPSVLRAHGLGARRNSYGLHKISMDIDSPLYSGTLSKALSWSAENILTLSESRVEEEKNDYSPPSPSPVSSPATGSITPPCSQEPDAAAQGSSAQSSESENEVQNKQQKILSRVKFDAQWEQDEKEDVETKAVATSPDGRYLKFNIEIGRGSFKTVYKGLDTETTVEVAWCELQTRKLTKAERQRFSEEVEMLKCLQHPNIVRFHDSWKSTVKGLKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIGGCIRMNNDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKPGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKMVAKAIRDRVTAIKRQREKMRKQAEEHKKKQQQEAIEEEPEPPPVLPKVFDAVPVESPPQALTPLAPILSPVTSSVDSGVSSSYPAEPEEPEADQHFHMRHNSLSSANSDCETDGYLSSSGLQDPLEAASFNSQATPPATYNEGAPTGNGFPIAALRFPSSIAVSNSIENGNSRSPSGLNSPVDSYASDVTSGLSDGYEGLSEKSEKTAARKTAGKMFRRRARSRLRITGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIATVMVHNEFILPSEKEGFIHRMGDIIKRAESLMAKGQPGHASVHRLTQPAFHSGANSLSSSQPNLHSHNRPRTSSSSSLPDFSVANPRAGLGGPSGDIFGADITSPERPLIRSQSFHNSPGSPLHHHHHYHPAPALPHHQHYHLPYSNFPFPYPGSPGSPKPAHHPPLTRVASNPTFPSVHSPAPGSPNSLNEAPHPGSNDSITLSPPVTQHPNMWPPHTQPLFSLANVISMAMSMAQSFIPPTGLPTQGMVTYPGFHPQMSGPIPPQTGYPSVYPPHYQSPPGEFSYPSVGIPAQNNVYHRPEPQMETLPQSSNLNWQHPVSPPSMASTPPLVPQEQQAGFSNSPRLTQEDHLHGYAPPHTAQVQTQTELRSDVSSPNSLSDLSPESTKSTLSPFASQLLPSGAEMTTSSPKPKTPCKESGVQPAPVTVGRFQVTPSMDILAPPAPVVSAAAAESSGTPSESSTEEQVESETSLATSLTMSPPHPHPHRGPAGHLQEVDPQSGWASSPEQEQARVEEEEDEEEEVEEEEEEEEEVGDRQRLRKRNRRRAYSLSLLGTSADSGLSVTAAELEGRLWDGAAGSPQYSNALHHLWMMTYSRSTPYLSSDESDSDGEEILEELQELREKHLTEVQTLQAAQKREIEELYEKMGKVPPAGIVSPAAVLSRRQRRLSKGGGFPSSRRNSLQRLDILPLQGIIRRNSLGGSSSGSQDKPSKGVTFSSDIRM

Target ORF information:

RefSeq Version	XM_011616884.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011616884.2

ORF Insert Sequence:

ATGTCACACG CACAGCTTGA GATAGTAGGA CGCGGCTTCC CGGGACACCC TATGGACATG 
ACCGACGACT GCTTATCCCC CGTCCCATCC GTGCTGAGGG CGCACGGTTT GGGCGCACGG 
AGGAACTCGT ACGGTCTGCA TAAAATCAGC ATGGACATCG ATTCTCCTCT GTACAGCGGC 
ACACTTTCCA AAGCCTTGTC TTGGTCTGCT GAGAACATTC TGACACTGTC CGAGTCCAGA 
GTAGAGGAGG AAAAAAACGA CTACTCTCCG CCGTCTCCGT CGCCGGTGTC CAGCCCGGCC 
ACCGGCTCCA TCACGCCCCC CTGCAGCCAG GAGCCAGACG CCGCCGCGCA GGGATCCAGC 
GCACAGAGTT CGGAGTCTGA AAACGAAGTT CAGAATAAGC AGCAAAAGAT CCTCTCTCGC 
GTCAAGTTCG ATGCTCAGTG GGAGCAGGAC GAGAAAGAAG ACGTCGAGAC CAAAGCGGTG 
GCGACTTCGC CCGATGGAAG ATACCTCAAA TTCAACATTG AGATCGGGAG GGGCTCGTTC 
AAGACGGTCT ATAAGGGACT GGACACTGAA ACGACGGTGG AAGTGGCGTG GTGTGAGTTA 
CAGACTCGGA AGCTGACCAA GGCGGAGCGG CAGCGTTTCA GCGAGGAGGT GGAAATGCTG 
AAGTGCCTGC AGCACCCCAA CATCGTCCGC TTCCACGACT CCTGGAAGTC GACGGTGAAG 
GGCCTCAAAT GCATCATCCT GGTGACGGAA CTGATGACGT CGGGCACGCT CAAAACGTAC 
TTGAAGAGAT TCAAGGAGAT GAAGCTAAAG CTGCTCCAGC GTTGGAGTCG ACAAATCCTC 
AAAGGGCTTC ACTTCCTGCA CACGCGAACG CCTCCCATCA TCCACAGAGA CCTGAAGTGT 
GACAACATCT TCATCACGGG CCCCACTGGC TCTGTCAAGA TTGGAGATCT GGGACTTGCC 
ACTCTTAAAA GTGCCTCTTT TGCTAAAAGT GTCATTGGAA CGCCAGAGTT CATGGCCCCG 
GAGATGTACG AGGAGAAGTA CGACGAAGCG GTGGACGTCT ACGCCTTCGG AATGTGCATC 
CTGGAGATGG CCACCTCTGA GTATCCGTAC TCCGAGTGCC AAAACGCCGC CCAGATCTAC 
CGCAAAGTCA CCAGTGGAAT TAAACCAGAC AGTTTCTACA AAGTCAAAGT CCCGGAGCTC 
AAAGAGATCA TCGGGGGCTG CATTCGCATG AACAACGATG AAAGGTACAC CATTCAGGAC 
CTCTTGGATC ACCCCTTCTT CCAGGAGAAC AACGGCGTGC ACGTGGAGCT GGCGGAGGAG 
GACGACATGG TGAAGCCAGG CCTGAAGCTG TGGCTGCGCA TGGACGACAC AAAGAAGCTC 
CACGGGAAGT ACAAGGACAA CAACGCCATC GAGTTCCTCT TTGAGCTCTA CAAAGACGTG 
CCCGAGGAGG TGGCTCAGGA GATGGTGGTG CTCGGGTTCG TGTGCGAAGC CGACTTCAAA 
ATGGTGGCCA AGGCCATACG GGACAGGGTG ACAGCCATCA AGCGGCAAAG GGAGAAGATG 
AGGAAGCAGG CGGAGGAGCA TAAGAAGAAG CAGCAGCAGG AAGCCATAGA AGAAGAGCCG 
GAACCTCCGC CTGTTTTGCC CAAAGTTTTC GACGCCGTCC CGGTGGAGTC TCCACCCCAA 
GCCCTAACCC CGCTGGCTCC CATCCTGTCC CCGGTCACCA GCTCCGTGGA CTCTGGCGTC 
AGCTCCAGCT ACCCCGCAGA GCCGGAGGAG CCTGAGGCCG ACCAGCACTT CCACATGCGC 
CACAACAGCC TGTCATCTGC TAACTCTGAC TGCGAGACAG ACGGCTATCT GAGCTCCTCT 
GGTCTTCAGG ATCCACTGGA GGCCGCCAGC TTTAACTCGC AGGCCACACC TCCTGCCACA 
TACAACGAAG GCGCACCAAC TGGAAACGGT TTCCCCATCG CGGCTCTGCG CTTCCCCTCA 
AGCATCGCTG TATCCAACAG CATAGAGAAC GGAAACTCGA GATCTCCGAG TGGCCTCAAC 
TCACCTGTGG ACAGTTACGC CTCTGACGTG ACCTCGGGTT TGAGTGATGG CTACGAGGGC 
CTATCGGAGA AGAGCGAGAA AACGGCCGCC AGGAAAACTG CTGGGAAGAT GTTCAGAAGG 
AGGGCGCGAT CAAGGCTGCG CATCACAGGG CTCTCTGATA AAGTGGACCG GGTGGTGGAG 
TGCCAGCTGC AGACGCACAA CGACAAGATG GTGACGTTCA AGTTCGACCT GGACGGAGAC 
AATCCCGAAG ACATCGCCAC TGTCATGGTG CACAATGAGT TCATCCTGCC GTCGGAGAAA 
GAGGGCTTCA TCCACCGTAT GGGGGACATC ATCAAGCGTG CAGAGTCTCT GATGGCCAAA 
GGGCAGCCAG GCCACGCCAG CGTGCACCGC CTCACACAGC CCGCTTTCCA CAGTGGGGCT 
AATTCACTGT CTTCGTCACA GCCTAATCTG CACAGCCACA ACCGCCCTCG CACCAGCTCC 
TCCTCATCTC TACCTGACTT TAGCGTGGCT AACCCAAGAG CTGGGCTGGG TGGCCCCAGT 
GGCGACATCT TCGGTGCAGA CATTACTTCA CCTGAGCGAC CTTTAATACG CTCACAGTCT 
TTCCACAACA GCCCAGGTTC TCCTCTCCAT CATCACCACC ACTACCATCC TGCACCTGCC 
CTGCCGCACC ACCAGCATTA CCACTTGCCT TACAGTAACT TCCCATTCCC GTACCCCGGC 
TCTCCAGGCT CCCCCAAGCC GGCCCACCAC CCACCGCTCA CACGTGTAGC CAGTAACCCC 
ACCTTCCCTT CCGTTCACTC CCCTGCCCCC GGTTCGCCCA ATTCCCTCAA TGAGGCCCCG 
CATCCAGGTA GCAACGACAG TATCACCCTG TCCCCACCTG TGACTCAGCA TCCCAACATG 
TGGCCCCCCC ACACGCAGCC CCTTTTTTCT TTAGCAAACG TGATCTCCAT GGCCATGAGC 
ATGGCCCAGT CTTTCATCCC TCCCACTGGC CTGCCCACCC AGGGGATGGT CACCTATCCA 
GGCTTTCACC CACAGATGTC TGGTCCCATT CCCCCACAGA CTGGTTACCC CTCCGTCTAC 
CCACCTCATT ACCAGAGCCC ACCAGGAGAG TTCTCTTACC CCTCAGTAGG CATCCCAGCA 
CAGAACAATG TCTACCACAG GCCTGAGCCT CAGATGGAAA CTTTGCCCCA GTCCAGTAAC 
CTCAACTGGC AGCATCCTGT CTCACCCCCC TCAATGGCTT CCACCCCTCC CCTGGTTCCC 
CAGGAGCAGC AGGCGGGATT TTCCAATTCC CCCAGACTCA CCCAGGAGGA CCATCTCCAT 
GGATATGCGC CCCCACATAC AGCGCAGGTC CAAACCCAGA CTGAACTCAG ATCAGATGTT 
TCCAGCCCAA ACTCTTTATC AGATCTTTCT CCAGAGAGCA CAAAAAGCAC CTTGTCACCC 
TTTGCCAGTC AGCTCCTCCC CAGTGGAGCT GAGATGACCA CCAGTTCCCC CAAACCAAAA 
ACACCTTGTA AGGAATCTGG AGTCCAGCCT GCACCAGTCA CTGTTGGGAG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCT GGCGCCTCCT GCCCCTGTCG TGTCCGCAGC GGCTGCAGAG 
AGCAGTGGCA CCCCATCAGA GAGCAGCACA GAGGAGCAGG TCGAGTCCGA GACCAGCCTG 
GCCACCTCCC TCACCATGTC CCCTCCTCAT CCACACCCCC ACAGAGGGCC TGCCGGACAC 
CTCCAGGAGG TCGACCCACA GTCTGGATGG GCTAGCAGCC CAGAGCAGGA ACAGGCCCGA 
GTGGAAGAGG AGGAAGATGA GGAGGAGGAG GTGGAAGAGG AAGAGGAGGA GGAGGAGGAA 
GTGGGGGACC GACAGAGGCT GAGGAAGAGA AATCGCAGGA GGGCCTACAG CCTGAGTTTG 
CTGGGGACGT CGGCAGACAG TGGGCTCTCT GTGACGGCCG CTGAGCTGGA GGGTCGACTG 
TGGGATGGAG CGGCCGGCAG CCCCCAGTAC AGCAACGCCC TTCACCATTT ATGGATGATG 
ACCTACAGTC GCAGCACTCC GTACCTGAGC AGCGATGAAT CCGACAGCGA TGGCGAGGAA 
ATACTGGAGG AGCTTCAGGA GCTCAGAGAG AAACATCTGA CGGAGGTCCA GACCCTGCAG 
GCTGCCCAGA AGAGAGAGAT CGAGGAGTTG TACGAGAAGA TGGGGAAGGT CCCTCCGGCG 
GGTATCGTCT CTCCCGCCGC CGTGCTGTCC AGACGGCAGC GTCGCCTGTC AAAGGGTGGC 
GGCTTCCCCT CCTCTCGCAG GAACAGCCTG CAGCGCTTGG ACATCCTTCC GCTCCAGGGT 
ATCATCAGGA GAAACTCTTT AGGTGGCAGC AGCAGCGGCT CCCAGGATAA ACCAAGCAAA 
GGCGTCACCT TTTCCAGCGA TATCAGAATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk4 ( XM_011617375.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011615677.2 Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X2, mRNA.

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CloneID	OTo43122
Clone ID Related Accession (Same CDS sequence)	XM_011617375.2
Accession Version	XM_011617375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	serine/threonine-protein kinase WNK4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011617375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACACG CACAGCTTGA GATAGTAGGA CGCGGCTTCC CGGGACACCC TATGGACATG 
ACCGACGACT GCTTATCCCC CGTCCCATCC GTGCTGAGGG CGCACGGTTT GGGCGCACGG 
AGGAACTCGT ACGGTCTGCA TAAAATCAGC ATGGACATCG ATTCTCCTCT GTACAGCGGC 
ACACTTTCCA AAGCCTTGTC TTGGTCTGCT GAGAACATTC TGACACTGTC CGAGTCCAGA 
GTAGAGGAGG AAAAAAACGA CTACTCTCCG CCGTCTCCGT CGCCGGTGTC CAGCCCGGCC 
ACCGGCTCCA TCACGCCCCC CTGCAGCCAG GAGCCAGACG CCGCCGCGCA GGGATCCAGC 
GCACAGAGTT CGGAGTCTGA AAACGAAGTT CAGAATAAGC AGCAAAAGAT CCTCTCTCGC 
GTCAAGTTCG ATGCTCAGTG GGAGCAGGAC GAGAAAGAAG ACGTCGAGAC CAAAGCGGTG 
GCGACTTCGC CCGATGGAAG ATACCTCAAA TTCAACATTG AGATCGGGAG GGGCTCGTTC 
AAGACGGTCT ATAAGGGACT GGACACTGAA ACGACGGTGG AAGTGGCGTG GTGTGAGTTA 
CAGACTCGGA AGCTGACCAA GGCGGAGCGG CAGCGTTTCA GCGAGGAGGT GGAAATGCTG 
AAGTGCCTGC AGCACCCCAA CATCGTCCGC TTCCACGACT CCTGGAAGTC GACGGTGAAG 
GGCCTCAAAT GCATCATCCT GGTGACGGAA CTGATGACGT CGGGCACGCT CAAAACGTAC 
TTGAAGAGAT TCAAGGAGAT GAAGCTAAAG CTGCTCCAGC GTTGGAGTCG ACAAATCCTC 
AAAGGGCTTC ACTTCCTGCA CACGCGAACG CCTCCCATCA TCCACAGAGA CCTGAAGTGT 
GACAACATCT TCATCACGGG CCCCACTGGC TCTGTCAAGA TTGGAGATCT GGGACTTGCC 
ACTCTTAAAA GTGCCTCTTT TGCTAAAAGT GTCATTGGAA CGCCAGAGTT CATGGCCCCG 
GAGATGTACG AGGAGAAGTA CGACGAAGCG GTGGACGTCT ACGCCTTCGG AATGTGCATC 
CTGGAGATGG CCACCTCTGA GTATCCGTAC TCCGAGTGCC AAAACGCCGC CCAGATCTAC 
CGCAAAGTCA CCAGTGGAAT TAAACCAGAC AGTTTCTACA AAGTCAAAGT CCCGGAGCTC 
AAAGAGATCA TCGGGGGCTG CATTCGCATG AACAACGATG AAAGGTACAC CATTCAGGAC 
CTCTTGGATC ACCCCTTCTT CCAGGAGAAC AACGGCGTGC ACGTGGAGCT GGCGGAGGAG 
GACGACATGG TGAAGCCAGG CCTGAAGCTG TGGCTGCGCA TGGACGACAC AAAGAAGCTC 
CACGGGAAGT ACAAGGACAA CAACGCCATC GAGTTCCTCT TTGAGCTCTA CAAAGACGTG 
CCCGAGGAGG TGGCTCAGGA GATGGTGGTG CTCGGGTTCG TGTGCGAAGC CGACTTCAAA 
ATGGTGGCCA AGGCCATACG GGACAGGGTG ACAGCCATCA AGCGGCAAAG GGAGAAGATG 
AGGAAGCAGG CGGAGGAGCA TAAGAAGAAG CAGCAGCAGG AAGCCATAGA AGAAGAGCCG 
GAACCTCCGC CTGTTTTGCC CAAAGTTTTC GACGCCGTCC CGGTGGAGTC TCCACCCCAA 
GCCCTAACCC CGCTGGCTCC CATCCTGTCC CCGGTCACCA GCTCCGTGGA CTCTGGCGTC 
AGCTCCAGCT ACCCCGCAGA GCCGGAGGAG CCTGAGGCCG ACCAGCACTT CCACATGCGC 
CACAACAGCC TGTCATCTGC TAACTCTGAC TGCGAGACAG ACGGCTATCT GAGCTCCTCT 
GGTCTTCAGG ATCCACTGGA GGCCGCCAGC TTTAACTCGC AGGCCACACC TCCTGCCACA 
TACAACGAAG GCGCACCAAC TGGAAACGGT TTCCCCATCG CGGCTCTGCG CTTCCCCTCA 
AGCATCGCTG TATCCAACAG CATAGAGAAC GGAAACTCGA GATCTCCGAG TGGCCTCAAC 
TCACCTGTGG ACAGTTACGC CTCTGACGTG ACCTCGGGTT TGAGTGATGG CTACGAGGGC 
CTATCGGAGA AGAGCGAGAA AACGGCCGCC AGGAAAACTG CTGGGAAGAT GTTCAGAAGG 
AGGGCGCGAT CAAGGCTGCG CATCACAGGG CTCTCTGATA AAGTGGACCG GGTGGTGGAG 
TGCCAGCTGC AGACGCACAA CGACAAGATG GTGACGTTCA AGTTCGACCT GGACGGAGAC 
AATCCCGAAG ACATCGCCAC TGTCATGGTG CACAATGAGT TCATCCTGCC GTCGGAGAAA 
GAGGGCTTCA TCCACCGTAT GGGGGACATC ATCAAGCGTG CAGAGTCTCT GATGGCCAAA 
GGGCAGCCAG GCCACGCCAG CGTGCACCGC CTCACACAGC CCGCTTTCCA CAGTGGGGCT 
AATTCACTGT CTTCGTCACA GCCTAATCTG CACAGCCACA ACCGCCCTCG CACCAGCTCC 
TCCTCATCTC TACCTGGTTC TCCTCTCCAT CATCACCACC ACTACCATCC TGCACCTGCC 
CTGCCGCACC ACCAGCATTA CCACTTGCCT TACAGTAACT TCCCATTCCC GTACCCCGGC 
TCTCCAGGCT CCCCCAAGCC GGCCCACCAC CCACCGCTCA CACGTGTAGC CAGTAACCCC 
ACCTTCCCTT CCGTTCACTC CCCTGCCCCC GGTTCGCCCA ATTCCCTCAA TGAGGCCCCG 
CATCCAGGTA GCAACGACAG TATCACCCTG TCCCCACCTG TGACTCAGCA TCCCAACATG 
TGGCCCCCCC ACACGCAGCC CCTTTTTTCT TTAGCAAACG TGATCTCCAT GGCCATGAGC 
ATGGCCCAGT CTTTCATCCC TCCCACTGGC CTGCCCACCC AGGGGATGGT CACCTATCCA 
GGCTTTCACC CACAGATGTC TGGTCCCATT CCCCCACAGA CTGGTTACCC CTCCGTCTAC 
CCACCTCATT ACCAGAGCCC ACCAGGAGAG TTCTCTTACC CCTCAGTAGG CATCCCAGCA 
CAGAACAATG TCTACCACAG GCCTGAGCCT CAGATGGAAA CTTTGCCCCA GTCCAGTAAC 
CTCAACTGGC AGCATCCTGT CTCACCCCCC TCAATGGCTT CCACCCCTCC CCTGGTTCCC 
CAGGAGCAGC AGGCGGGATT TTCCAATTCC CCCAGACTCA CCCAGGAGGA CCATCTCCAT 
GGATATGCGC CCCCACATAC AGCGCAGGTC CAAACCCAGA CTGAACTCAG ATCAGATGTT 
TCCAGCCCAA ACTCTTTATC AGATCTTTCT CCAGAGAGCA CAAAAAGCAC CTTGTCACCC 
TTTGCCAGTC AGCTCCTCCC CAGTGGAGCT GAGATGACCA CCAGTTCCCC CAAACCAAAA 
ACACCTTGTA AGGAATCTGG AGTCCAGCCT GCACCAGTCA CTGTTGGGAG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCT GGCGCCTCCT GCCCCTGTCG TGTCCGCAGC GGCTGCAGAG 
AGCAGTGGCA CCCCATCAGA GAGCAGCACA GAGGAGCAGG TCGAGTCCGA GACCAGCCTG 
GCCACCTCCC TCACCATGTC CCCTCCTCAT CCACACCCCC ACAGAGGGCC TGCCGGACAC 
CTCCAGGAGG TCGACCCACA GTCTGGATGG GCTAGCAGCC CAGAGCAGGA ACAGGCCCGA 
GTGGAAGAGG AGGAAGATGA GGAGGAGGAG GTGGAAGAGG AAGAGGAGGA GGAGGAGGAA 
GTGGGGGACC GACAGAGGCT GAGGAAGAGA AATCGCAGGA GGGCCTACAG CCTGAGTTTG 
CTGGGGACGT CGGCAGACAG TGGGCTCTCT GTGACGGCCG CTGAGCTGGA GGGTCGACTG 
TGGGATGGAG CGGCCGGCAG CCCCCAGTAC AGCAACGCCC TTCACCATTT ATGGATGATG 
ACCTACAGTC GCAGCACTCC GTACCTGAGC AGCGATGAAT CCGACAGCGA TGGCGAGGAA 
ATACTGGAGG AGCTTCAGGA GCTCAGAGAG AAACATCTGA CGGAGGTCCA GACCCTGCAG 
GCTGCCCAGA AGAGAGAGAT CGAGGAGTTG TACGAGAAGA TGGGGAAGGT CCCTCCGGCG 
GGTATCGTCT CTCCCGCCGC CGTGCTGTCC AGACGGCAGC GTCGCCTGTC AAAGGGTGGC 
GGCTTCCCCT CCTCTCGCAG GAACAGCCTG CAGCGCTTGG ACATCCTTCC GCTCCAGGGT 
ATCATCAGGA GAAACTCTTT AGGTGGCAGC AGCAGCGGCT CCCAGGATAA ACCAAGCAAA 
GGCGTCACCT TTTCCAGCGA TATCAGAATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615677.2
CDS	222..4574
Translation	MSHAQLEIVGRGFPGHPMDMTDDCLSPVPSVLRAHGLGARRNSYGLHKISMDIDSPLYSGTLSKALSWSAENILTLSESRVEEEKNDYSPPSPSPVSSPATGSITPPCSQEPDAAAQGSSAQSSESENEVQNKQQKILSRVKFDAQWEQDEKEDVETKAVATSPDGRYLKFNIEIGRGSFKTVYKGLDTETTVEVAWCELQTRKLTKAERQRFSEEVEMLKCLQHPNIVRFHDSWKSTVKGLKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIGGCIRMNNDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKPGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKMVAKAIRDRVTAIKRQREKMRKQAEEHKKKQQQEAIEEEPEPPPVLPKVFDAVPVESPPQALTPLAPILSPVTSSVDSGVSSSYPAEPEEPEADQHFHMRHNSLSSANSDCETDGYLSSSGLQDPLEAASFNSQATPPATYNEGAPTGNGFPIAALRFPSSIAVSNSIENGNSRSPSGLNSPVDSYASDVTSGLSDGYEGLSEKSEKTAARKTAGKMFRRRARSRLRITGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIATVMVHNEFILPSEKEGFIHRMGDIIKRAESLMAKGQPGHASVHRLTQPAFHSGANSLSSSQPNLHSHNRPRTSSSSSLPGSPLHHHHHYHPAPALPHHQHYHLPYSNFPFPYPGSPGSPKPAHHPPLTRVASNPTFPSVHSPAPGSPNSLNEAPHPGSNDSITLSPPVTQHPNMWPPHTQPLFSLANVISMAMSMAQSFIPPTGLPTQGMVTYPGFHPQMSGPIPPQTGYPSVYPPHYQSPPGEFSYPSVGIPAQNNVYHRPEPQMETLPQSSNLNWQHPVSPPSMASTPPLVPQEQQAGFSNSPRLTQEDHLHGYAPPHTAQVQTQTELRSDVSSPNSLSDLSPESTKSTLSPFASQLLPSGAEMTTSSPKPKTPCKESGVQPAPVTVGRFQVTPSMDILAPPAPVVSAAAAESSGTPSESSTEEQVESETSLATSLTMSPPHPHPHRGPAGHLQEVDPQSGWASSPEQEQARVEEEEDEEEEVEEEEEEEEEVGDRQRLRKRNRRRAYSLSLLGTSADSGLSVTAAELEGRLWDGAAGSPQYSNALHHLWMMTYSRSTPYLSSDESDSDGEEILEELQELREKHLTEVQTLQAAQKREIEELYEKMGKVPPAGIVSPAAVLSRRQRRLSKGGGFPSSRRNSLQRLDILPLQGIIRRNSLGGSSSGSQDKPSKGVTFSSDIRM

Target ORF information:

RefSeq Version	XM_011617375.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617375.2

ORF Insert Sequence:

ATGTCACACG CACAGCTTGA GATAGTAGGA CGCGGCTTCC CGGGACACCC TATGGACATG 
ACCGACGACT GCTTATCCCC CGTCCCATCC GTGCTGAGGG CGCACGGTTT GGGCGCACGG 
AGGAACTCGT ACGGTCTGCA TAAAATCAGC ATGGACATCG ATTCTCCTCT GTACAGCGGC 
ACACTTTCCA AAGCCTTGTC TTGGTCTGCT GAGAACATTC TGACACTGTC CGAGTCCAGA 
GTAGAGGAGG AAAAAAACGA CTACTCTCCG CCGTCTCCGT CGCCGGTGTC CAGCCCGGCC 
ACCGGCTCCA TCACGCCCCC CTGCAGCCAG GAGCCAGACG CCGCCGCGCA GGGATCCAGC 
GCACAGAGTT CGGAGTCTGA AAACGAAGTT CAGAATAAGC AGCAAAAGAT CCTCTCTCGC 
GTCAAGTTCG ATGCTCAGTG GGAGCAGGAC GAGAAAGAAG ACGTCGAGAC CAAAGCGGTG 
GCGACTTCGC CCGATGGAAG ATACCTCAAA TTCAACATTG AGATCGGGAG GGGCTCGTTC 
AAGACGGTCT ATAAGGGACT GGACACTGAA ACGACGGTGG AAGTGGCGTG GTGTGAGTTA 
CAGACTCGGA AGCTGACCAA GGCGGAGCGG CAGCGTTTCA GCGAGGAGGT GGAAATGCTG 
AAGTGCCTGC AGCACCCCAA CATCGTCCGC TTCCACGACT CCTGGAAGTC GACGGTGAAG 
GGCCTCAAAT GCATCATCCT GGTGACGGAA CTGATGACGT CGGGCACGCT CAAAACGTAC 
TTGAAGAGAT TCAAGGAGAT GAAGCTAAAG CTGCTCCAGC GTTGGAGTCG ACAAATCCTC 
AAAGGGCTTC ACTTCCTGCA CACGCGAACG CCTCCCATCA TCCACAGAGA CCTGAAGTGT 
GACAACATCT TCATCACGGG CCCCACTGGC TCTGTCAAGA TTGGAGATCT GGGACTTGCC 
ACTCTTAAAA GTGCCTCTTT TGCTAAAAGT GTCATTGGAA CGCCAGAGTT CATGGCCCCG 
GAGATGTACG AGGAGAAGTA CGACGAAGCG GTGGACGTCT ACGCCTTCGG AATGTGCATC 
CTGGAGATGG CCACCTCTGA GTATCCGTAC TCCGAGTGCC AAAACGCCGC CCAGATCTAC 
CGCAAAGTCA CCAGTGGAAT TAAACCAGAC AGTTTCTACA AAGTCAAAGT CCCGGAGCTC 
AAAGAGATCA TCGGGGGCTG CATTCGCATG AACAACGATG AAAGGTACAC CATTCAGGAC 
CTCTTGGATC ACCCCTTCTT CCAGGAGAAC AACGGCGTGC ACGTGGAGCT GGCGGAGGAG 
GACGACATGG TGAAGCCAGG CCTGAAGCTG TGGCTGCGCA TGGACGACAC AAAGAAGCTC 
CACGGGAAGT ACAAGGACAA CAACGCCATC GAGTTCCTCT TTGAGCTCTA CAAAGACGTG 
CCCGAGGAGG TGGCTCAGGA GATGGTGGTG CTCGGGTTCG TGTGCGAAGC CGACTTCAAA 
ATGGTGGCCA AGGCCATACG GGACAGGGTG ACAGCCATCA AGCGGCAAAG GGAGAAGATG 
AGGAAGCAGG CGGAGGAGCA TAAGAAGAAG CAGCAGCAGG AAGCCATAGA AGAAGAGCCG 
GAACCTCCGC CTGTTTTGCC CAAAGTTTTC GACGCCGTCC CGGTGGAGTC TCCACCCCAA 
GCCCTAACCC CGCTGGCTCC CATCCTGTCC CCGGTCACCA GCTCCGTGGA CTCTGGCGTC 
AGCTCCAGCT ACCCCGCAGA GCCGGAGGAG CCTGAGGCCG ACCAGCACTT CCACATGCGC 
CACAACAGCC TGTCATCTGC TAACTCTGAC TGCGAGACAG ACGGCTATCT GAGCTCCTCT 
GGTCTTCAGG ATCCACTGGA GGCCGCCAGC TTTAACTCGC AGGCCACACC TCCTGCCACA 
TACAACGAAG GCGCACCAAC TGGAAACGGT TTCCCCATCG CGGCTCTGCG CTTCCCCTCA 
AGCATCGCTG TATCCAACAG CATAGAGAAC GGAAACTCGA GATCTCCGAG TGGCCTCAAC 
TCACCTGTGG ACAGTTACGC CTCTGACGTG ACCTCGGGTT TGAGTGATGG CTACGAGGGC 
CTATCGGAGA AGAGCGAGAA AACGGCCGCC AGGAAAACTG CTGGGAAGAT GTTCAGAAGG 
AGGGCGCGAT CAAGGCTGCG CATCACAGGG CTCTCTGATA AAGTGGACCG GGTGGTGGAG 
TGCCAGCTGC AGACGCACAA CGACAAGATG GTGACGTTCA AGTTCGACCT GGACGGAGAC 
AATCCCGAAG ACATCGCCAC TGTCATGGTG CACAATGAGT TCATCCTGCC GTCGGAGAAA 
GAGGGCTTCA TCCACCGTAT GGGGGACATC ATCAAGCGTG CAGAGTCTCT GATGGCCAAA 
GGGCAGCCAG GCCACGCCAG CGTGCACCGC CTCACACAGC CCGCTTTCCA CAGTGGGGCT 
AATTCACTGT CTTCGTCACA GCCTAATCTG CACAGCCACA ACCGCCCTCG CACCAGCTCC 
TCCTCATCTC TACCTGGTTC TCCTCTCCAT CATCACCACC ACTACCATCC TGCACCTGCC 
CTGCCGCACC ACCAGCATTA CCACTTGCCT TACAGTAACT TCCCATTCCC GTACCCCGGC 
TCTCCAGGCT CCCCCAAGCC GGCCCACCAC CCACCGCTCA CACGTGTAGC CAGTAACCCC 
ACCTTCCCTT CCGTTCACTC CCCTGCCCCC GGTTCGCCCA ATTCCCTCAA TGAGGCCCCG 
CATCCAGGTA GCAACGACAG TATCACCCTG TCCCCACCTG TGACTCAGCA TCCCAACATG 
TGGCCCCCCC ACACGCAGCC CCTTTTTTCT TTAGCAAACG TGATCTCCAT GGCCATGAGC 
ATGGCCCAGT CTTTCATCCC TCCCACTGGC CTGCCCACCC AGGGGATGGT CACCTATCCA 
GGCTTTCACC CACAGATGTC TGGTCCCATT CCCCCACAGA CTGGTTACCC CTCCGTCTAC 
CCACCTCATT ACCAGAGCCC ACCAGGAGAG TTCTCTTACC CCTCAGTAGG CATCCCAGCA 
CAGAACAATG TCTACCACAG GCCTGAGCCT CAGATGGAAA CTTTGCCCCA GTCCAGTAAC 
CTCAACTGGC AGCATCCTGT CTCACCCCCC TCAATGGCTT CCACCCCTCC CCTGGTTCCC 
CAGGAGCAGC AGGCGGGATT TTCCAATTCC CCCAGACTCA CCCAGGAGGA CCATCTCCAT 
GGATATGCGC CCCCACATAC AGCGCAGGTC CAAACCCAGA CTGAACTCAG ATCAGATGTT 
TCCAGCCCAA ACTCTTTATC AGATCTTTCT CCAGAGAGCA CAAAAAGCAC CTTGTCACCC 
TTTGCCAGTC AGCTCCTCCC CAGTGGAGCT GAGATGACCA CCAGTTCCCC CAAACCAAAA 
ACACCTTGTA AGGAATCTGG AGTCCAGCCT GCACCAGTCA CTGTTGGGAG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCT GGCGCCTCCT GCCCCTGTCG TGTCCGCAGC GGCTGCAGAG 
AGCAGTGGCA CCCCATCAGA GAGCAGCACA GAGGAGCAGG TCGAGTCCGA GACCAGCCTG 
GCCACCTCCC TCACCATGTC CCCTCCTCAT CCACACCCCC ACAGAGGGCC TGCCGGACAC 
CTCCAGGAGG TCGACCCACA GTCTGGATGG GCTAGCAGCC CAGAGCAGGA ACAGGCCCGA 
GTGGAAGAGG AGGAAGATGA GGAGGAGGAG GTGGAAGAGG AAGAGGAGGA GGAGGAGGAA 
GTGGGGGACC GACAGAGGCT GAGGAAGAGA AATCGCAGGA GGGCCTACAG CCTGAGTTTG 
CTGGGGACGT CGGCAGACAG TGGGCTCTCT GTGACGGCCG CTGAGCTGGA GGGTCGACTG 
TGGGATGGAG CGGCCGGCAG CCCCCAGTAC AGCAACGCCC TTCACCATTT ATGGATGATG 
ACCTACAGTC GCAGCACTCC GTACCTGAGC AGCGATGAAT CCGACAGCGA TGGCGAGGAA 
ATACTGGAGG AGCTTCAGGA GCTCAGAGAG AAACATCTGA CGGAGGTCCA GACCCTGCAG 
GCTGCCCAGA AGAGAGAGAT CGAGGAGTTG TACGAGAAGA TGGGGAAGGT CCCTCCGGCG 
GGTATCGTCT CTCCCGCCGC CGTGCTGTCC AGACGGCAGC GTCGCCTGTC AAAGGGTGGC 
GGCTTCCCCT CCTCTCGCAG GAACAGCCTG CAGCGCTTGG ACATCCTTCC GCTCCAGGGT 
ATCATCAGGA GAAACTCTTT AGGTGGCAGC AGCAGCGGCT CCCAGGATAA ACCAAGCAAA 
GGCGTCACCT TTTCCAGCGA TATCAGAATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk4 ( XM_029850413.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029706273.1 Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo43123
Clone ID Related Accession (Same CDS sequence)	XM_029850413.1 , XM_029850410.1
Accession Version	XM_029850413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	serine/threonine-protein kinase WNK4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGA CTCGGAAGCT GACCAAGGCG GAGCGGCAGC GTTTCAGCGA GGAGGTGGAA 
ATGCTGAAGT GCCTGCAGCA CCCCAACATC GTCCGCTTCC ACGACTCCTG GAAGTCGACG 
GTGAAGGGCC TCAAATGCAT CATCCTGGTG ACGGAACTGA TGACGTCGGG CACGCTCAAA 
ACGTACTTGA AGAGATTCAA GGAGATGAAG CTAAAGCTGC TCCAGCGTTG GAGTCGACAA 
ATCCTCAAAG GGCTTCACTT CCTGCACACG CGAACGCCTC CCATCATCCA CAGAGACCTG 
AAGTGTGACA ACATCTTCAT CACGGGCCCC ACTGGCTCTG TCAAGATTGG AGATCTGGGA 
CTTGCCACTC TTAAAAGTGC CTCTTTTGCT AAAAGTGTCA TTGGAACGCC AGAGTTCATG 
GCCCCGGAGA TGTACGAGGA GAAGTACGAC GAAGCGGTGG ACGTCTACGC CTTCGGAATG 
TGCATCCTGG AGATGGCCAC CTCTGAGTAT CCGTACTCCG AGTGCCAAAA CGCCGCCCAG 
ATCTACCGCA AAGTCACCAG TGGAATTAAA CCAGACAGTT TCTACAAAGT CAAAGTCCCG 
GAGCTCAAAG AGATCATCGG GGGCTGCATT CGCATGAACA ACGATGAAAG GTACACCATT 
CAGGACCTCT TGGATCACCC CTTCTTCCAG GAGAACAACG GCGTGCACGT GGAGCTGGCG 
GAGGAGGACG ACATGGTGAA GCCAGGCCTG AAGCTGTGGC TGCGCATGGA CGACACAAAG 
AAGCTCCACG GGAAGTACAA GGACAACAAC GCCATCGAGT TCCTCTTTGA GCTCTACAAA 
GACGTGCCCG AGGAGGTGGC TCAGGAGATG GTGGTGCTCG GGTTCGTGTG CGAAGCCGAC 
TTCAAAATGG TGGCCAAGGC CATACGGGAC AGGGTGACAG CCATCAAGCG GCAAAGGGAG 
AAGATGAGGA AGCAGGCGGA GGAGCATAAG AAGAAGCAGC AGCAGGAAGC CATAGAAGAA 
GAGCCGGAAC CTCCGCCTGT TTTGCCCAAA GTTTTCGACG CCGTCCCGGT GGAGTCTCCA 
CCCCAAGCCC TAACCCCGCT GGCTCCCATC CTGTCCCCGG TCACCAGCTC CGTGGACTCT 
GGCGTCAGCT CCAGCTACCC CGCAGAGCCG GAGGAGCCTG AGGCCGACCA GCACTTCCAC 
ATGCGCCACA ACAGCCTGTC ATCTGCTAAC TCTGACTGCG AGACAGACGG CTATCTGAGC 
TCCTCTGGTC TTCAGGATCC ACTGGAGGCC GCCAGCTTTA ACTCGCAGGC CACACCTCCT 
GCCACATACA ACGAAGGCGC ACCAACTGGA AACGGTTTCC CCATCGCGGC TCTGCGCTTC 
CCCTCAAGCA TCGCTGTATC CAACAGCATA GAGAACGGAA ACTCGAGATC TCCGAGTGGC 
CTCAACTCAC CTGTGGACAG TTACGCCTCT GACGTGACCT CGGGTTTGAG TGATGGCTAC 
GAGGGCCTAT CGGAGAAGAG CGAGAAAACG GCCGCCAGGA AAACTGCTGG GAAGATGTTC 
AGAAGGAGGG CGCGATCAAG GCTGCGCATC ACAGGGCTCT CTGATAAAGT GGACCGGGTG 
GTGGAGTGCC AGCTGCAGAC GCACAACGAC AAGATGGTGA CGTTCAAGTT CGACCTGGAC 
GGAGACAATC CCGAAGACAT CGCCACTGTC ATGGTGCACA ATGAGTTCAT CCTGCCGTCG 
GAGAAAGAGG GCTTCATCCA CCGTATGGGG GACATCATCA AGCGTGCAGA GTCTCTGATG 
GCCAAAGGGC AGCCAGGCCA CGCCAGCGTG CACCGCCTCA CACAGCCCGC TTTCCACAGT 
GGGGCTAATT CACTGTCTTC GTCACAGCCT AATCTGCACA GCCACAACCG CCCTCGCACC 
AGCTCCTCCT CATCTCTACC TGACTTTAGC GTGGCTAACC CAAGAGCTGG GCTGGGTGGC 
CCCAGTGGCG ACATCTTCGG TGCAGACATT ACTTCACCTG AGCGACCTTT AATACGCTCA 
CAGTCTTTCC ACAACAGCCC AGGTTCTCCT CTCCATCATC ACCACCACTA CCATCCTGCA 
CCTGCCCTGC CGCACCACCA GCATTACCAC TTGCCTTACA GTAACTTCCC ATTCCCGTAC 
CCCGGCTCTC CAGGCTCCCC CAAGCCGGCC CACCACCCAC CGCTCACACG TGTAGCCAGT 
AACCCCACCT TCCCTTCCGT TCACTCCCCT GCCCCCGGTT CGCCCAATTC CCTCAATGAG 
GCCCCGCATC CAGGTAGCAA CGACAGTATC ACCCTGTCCC CACCTGTGAC TCAGCATCCC 
AACATGTGGC CCCCCCACAC GCAGCCCCTT TTTTCTTTAG CAAACGTGAT CTCCATGGCC 
ATGAGCATGG CCCAGTCTTT CATCCCTCCC ACTGGCCTGC CCACCCAGGG GATGGTCACC 
TATCCAGGCT TTCACCCACA GATGTCTGGT CCCATTCCCC CACAGACTGG TTACCCCTCC 
GTCTACCCAC CTCATTACCA GAGCCCACCA GGAGAGTTCT CTTACCCCTC AGTAGGCATC 
CCAGCACAGA ACAATGTCTA CCACAGGCCT GAGCCTCAGA TGGAAACTTT GCCCCAGTCC 
AGTAACCTCA ACTGGCAGCA TCCTGTCTCA CCCCCCTCAA TGGCTTCCAC CCCTCCCCTG 
GTTCCCCAGG AGCAGCAGGC GGGATTTTCC AATTCCCCCA GACTCACCCA GGAGGACCAT 
CTCCATGGAT ATGCGCCCCC ACATACAGCG CAGGTCCAAA CCCAGACTGA ACTCAGATCA 
GATGTTTCCA GCCCAAACTC TTTATCAGAT CTTTCTCCAG AGAGCACAAA AAGCACCTTG 
TCACCCTTTG CCAGTCAGCT CCTCCCCAGT GGAGCTGAGA TGACCACCAG TTCCCCCAAA 
CCAAAAACAC CTTGTAAGGA ATCTGGAGTC CAGCCTGCAC CAGTCACTGT TGGGAGATTC 
CAGGTGACGC CCAGCATGGA CATCCTGGCG CCTCCTGCCC CTGTCGTGTC CGCAGCGGCT 
GCAGAGAGCA GTGGCACCCC ATCAGAGAGC AGCACAGAGG AGCAGGTCGA GTCCGAGACC 
AGCCTGGCCA CCTCCCTCAC CATGTCCCCT CCTCATCCAC ACCCCCACAG AGGGCCTGCC 
GGACACCTCC AGGAGGTCGA CCCACAGTCT GGATGGGCTA GCAGCCCAGA GCAGGAACAG 
GCCCGAGTGG AAGAGGAGGA AGATGAGGAG GAGGAGGTGG AAGAGGAAGA GGAGGAGGAG 
GAGGAAGTGG GGGACCGACA GAGGCTGAGG AAGAGAAATC GCAGGAGGGC CTACAGCCTG 
AGTTTGCTGG GGACGTCGGC AGACAGTGGG CTCTCTGTGA CGGCCGCTGA GCTGGAGGGT 
CGACTGTGGG ATGGAGCGGC CGGCAGCCCC CAGTACAGCA ACGCCCTTCA CCATTTATGG 
ATGATGACCT ACAGTCGCAG CACTCCGTAC CTGAGCAGCG ATGAATCCGA CAGCGATGGC 
GAGGAAATAC TGGAGGAGCT TCAGGAGCTC AGAGAGAAAC ATCTGACGGA GGTCCAGACC 
CTGCAGGCTG CCCAGAAGAG AGAGATCGAG GAGTTGTACG AGAAGATGGG GAAGGTCCCT 
CCGGCGGGTA TCGTCTCTCC CGCCGCCGTG CTGTCCAGAC GGCAGCGTCG CCTGTCAAAG 
GGTGGCGGCT TCCCCTCCTC TCGCAGGAAC AGCCTGCAGC GCTTGGACAT CCTTCCGCTC 
CAGGGTATCA TCAGGAGAAA CTCTTTAGGT GGCAGCAGCA GCGGCTCCCA GGATAAACCA 
AGCAAAGGCG TCACCTTTTC CAGCGATATC AGAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029706273.1
CDS	266..4144
Translation	MIATRKLTKAERQRFSEEVEMLKCLQHPNIVRFHDSWKSTVKGLKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIGGCIRMNNDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKPGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKMVAKAIRDRVTAIKRQREKMRKQAEEHKKKQQQEAIEEEPEPPPVLPKVFDAVPVESPPQALTPLAPILSPVTSSVDSGVSSSYPAEPEEPEADQHFHMRHNSLSSANSDCETDGYLSSSGLQDPLEAASFNSQATPPATYNEGAPTGNGFPIAALRFPSSIAVSNSIENGNSRSPSGLNSPVDSYASDVTSGLSDGYEGLSEKSEKTAARKTAGKMFRRRARSRLRITGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIATVMVHNEFILPSEKEGFIHRMGDIIKRAESLMAKGQPGHASVHRLTQPAFHSGANSLSSSQPNLHSHNRPRTSSSSSLPDFSVANPRAGLGGPSGDIFGADITSPERPLIRSQSFHNSPGSPLHHHHHYHPAPALPHHQHYHLPYSNFPFPYPGSPGSPKPAHHPPLTRVASNPTFPSVHSPAPGSPNSLNEAPHPGSNDSITLSPPVTQHPNMWPPHTQPLFSLANVISMAMSMAQSFIPPTGLPTQGMVTYPGFHPQMSGPIPPQTGYPSVYPPHYQSPPGEFSYPSVGIPAQNNVYHRPEPQMETLPQSSNLNWQHPVSPPSMASTPPLVPQEQQAGFSNSPRLTQEDHLHGYAPPHTAQVQTQTELRSDVSSPNSLSDLSPESTKSTLSPFASQLLPSGAEMTTSSPKPKTPCKESGVQPAPVTVGRFQVTPSMDILAPPAPVVSAAAAESSGTPSESSTEEQVESETSLATSLTMSPPHPHPHRGPAGHLQEVDPQSGWASSPEQEQARVEEEEDEEEEVEEEEEEEEEVGDRQRLRKRNRRRAYSLSLLGTSADSGLSVTAAELEGRLWDGAAGSPQYSNALHHLWMMTYSRSTPYLSSDESDSDGEEILEELQELREKHLTEVQTLQAAQKREIEELYEKMGKVPPAGIVSPAAVLSRRQRRLSKGGGFPSSRRNSLQRLDILPLQGIIRRNSLGGSSSGSQDKPSKGVTFSSDIRM

Target ORF information:

RefSeq Version	XM_029850413.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029850413.1

ORF Insert Sequence:

ATGATTGCGA CTCGGAAGCT GACCAAGGCG GAGCGGCAGC GTTTCAGCGA GGAGGTGGAA 
ATGCTGAAGT GCCTGCAGCA CCCCAACATC GTCCGCTTCC ACGACTCCTG GAAGTCGACG 
GTGAAGGGCC TCAAATGCAT CATCCTGGTG ACGGAACTGA TGACGTCGGG CACGCTCAAA 
ACGTACTTGA AGAGATTCAA GGAGATGAAG CTAAAGCTGC TCCAGCGTTG GAGTCGACAA 
ATCCTCAAAG GGCTTCACTT CCTGCACACG CGAACGCCTC CCATCATCCA CAGAGACCTG 
AAGTGTGACA ACATCTTCAT CACGGGCCCC ACTGGCTCTG TCAAGATTGG AGATCTGGGA 
CTTGCCACTC TTAAAAGTGC CTCTTTTGCT AAAAGTGTCA TTGGAACGCC AGAGTTCATG 
GCCCCGGAGA TGTACGAGGA GAAGTACGAC GAAGCGGTGG ACGTCTACGC CTTCGGAATG 
TGCATCCTGG AGATGGCCAC CTCTGAGTAT CCGTACTCCG AGTGCCAAAA CGCCGCCCAG 
ATCTACCGCA AAGTCACCAG TGGAATTAAA CCAGACAGTT TCTACAAAGT CAAAGTCCCG 
GAGCTCAAAG AGATCATCGG GGGCTGCATT CGCATGAACA ACGATGAAAG GTACACCATT 
CAGGACCTCT TGGATCACCC CTTCTTCCAG GAGAACAACG GCGTGCACGT GGAGCTGGCG 
GAGGAGGACG ACATGGTGAA GCCAGGCCTG AAGCTGTGGC TGCGCATGGA CGACACAAAG 
AAGCTCCACG GGAAGTACAA GGACAACAAC GCCATCGAGT TCCTCTTTGA GCTCTACAAA 
GACGTGCCCG AGGAGGTGGC TCAGGAGATG GTGGTGCTCG GGTTCGTGTG CGAAGCCGAC 
TTCAAAATGG TGGCCAAGGC CATACGGGAC AGGGTGACAG CCATCAAGCG GCAAAGGGAG 
AAGATGAGGA AGCAGGCGGA GGAGCATAAG AAGAAGCAGC AGCAGGAAGC CATAGAAGAA 
GAGCCGGAAC CTCCGCCTGT TTTGCCCAAA GTTTTCGACG CCGTCCCGGT GGAGTCTCCA 
CCCCAAGCCC TAACCCCGCT GGCTCCCATC CTGTCCCCGG TCACCAGCTC CGTGGACTCT 
GGCGTCAGCT CCAGCTACCC CGCAGAGCCG GAGGAGCCTG AGGCCGACCA GCACTTCCAC 
ATGCGCCACA ACAGCCTGTC ATCTGCTAAC TCTGACTGCG AGACAGACGG CTATCTGAGC 
TCCTCTGGTC TTCAGGATCC ACTGGAGGCC GCCAGCTTTA ACTCGCAGGC CACACCTCCT 
GCCACATACA ACGAAGGCGC ACCAACTGGA AACGGTTTCC CCATCGCGGC TCTGCGCTTC 
CCCTCAAGCA TCGCTGTATC CAACAGCATA GAGAACGGAA ACTCGAGATC TCCGAGTGGC 
CTCAACTCAC CTGTGGACAG TTACGCCTCT GACGTGACCT CGGGTTTGAG TGATGGCTAC 
GAGGGCCTAT CGGAGAAGAG CGAGAAAACG GCCGCCAGGA AAACTGCTGG GAAGATGTTC 
AGAAGGAGGG CGCGATCAAG GCTGCGCATC ACAGGGCTCT CTGATAAAGT GGACCGGGTG 
GTGGAGTGCC AGCTGCAGAC GCACAACGAC AAGATGGTGA CGTTCAAGTT CGACCTGGAC 
GGAGACAATC CCGAAGACAT CGCCACTGTC ATGGTGCACA ATGAGTTCAT CCTGCCGTCG 
GAGAAAGAGG GCTTCATCCA CCGTATGGGG GACATCATCA AGCGTGCAGA GTCTCTGATG 
GCCAAAGGGC AGCCAGGCCA CGCCAGCGTG CACCGCCTCA CACAGCCCGC TTTCCACAGT 
GGGGCTAATT CACTGTCTTC GTCACAGCCT AATCTGCACA GCCACAACCG CCCTCGCACC 
AGCTCCTCCT CATCTCTACC TGACTTTAGC GTGGCTAACC CAAGAGCTGG GCTGGGTGGC 
CCCAGTGGCG ACATCTTCGG TGCAGACATT ACTTCACCTG AGCGACCTTT AATACGCTCA 
CAGTCTTTCC ACAACAGCCC AGGTTCTCCT CTCCATCATC ACCACCACTA CCATCCTGCA 
CCTGCCCTGC CGCACCACCA GCATTACCAC TTGCCTTACA GTAACTTCCC ATTCCCGTAC 
CCCGGCTCTC CAGGCTCCCC CAAGCCGGCC CACCACCCAC CGCTCACACG TGTAGCCAGT 
AACCCCACCT TCCCTTCCGT TCACTCCCCT GCCCCCGGTT CGCCCAATTC CCTCAATGAG 
GCCCCGCATC CAGGTAGCAA CGACAGTATC ACCCTGTCCC CACCTGTGAC TCAGCATCCC 
AACATGTGGC CCCCCCACAC GCAGCCCCTT TTTTCTTTAG CAAACGTGAT CTCCATGGCC 
ATGAGCATGG CCCAGTCTTT CATCCCTCCC ACTGGCCTGC CCACCCAGGG GATGGTCACC 
TATCCAGGCT TTCACCCACA GATGTCTGGT CCCATTCCCC CACAGACTGG TTACCCCTCC 
GTCTACCCAC CTCATTACCA GAGCCCACCA GGAGAGTTCT CTTACCCCTC AGTAGGCATC 
CCAGCACAGA ACAATGTCTA CCACAGGCCT GAGCCTCAGA TGGAAACTTT GCCCCAGTCC 
AGTAACCTCA ACTGGCAGCA TCCTGTCTCA CCCCCCTCAA TGGCTTCCAC CCCTCCCCTG 
GTTCCCCAGG AGCAGCAGGC GGGATTTTCC AATTCCCCCA GACTCACCCA GGAGGACCAT 
CTCCATGGAT ATGCGCCCCC ACATACAGCG CAGGTCCAAA CCCAGACTGA ACTCAGATCA 
GATGTTTCCA GCCCAAACTC TTTATCAGAT CTTTCTCCAG AGAGCACAAA AAGCACCTTG 
TCACCCTTTG CCAGTCAGCT CCTCCCCAGT GGAGCTGAGA TGACCACCAG TTCCCCCAAA 
CCAAAAACAC CTTGTAAGGA ATCTGGAGTC CAGCCTGCAC CAGTCACTGT TGGGAGATTC 
CAGGTGACGC CCAGCATGGA CATCCTGGCG CCTCCTGCCC CTGTCGTGTC CGCAGCGGCT 
GCAGAGAGCA GTGGCACCCC ATCAGAGAGC AGCACAGAGG AGCAGGTCGA GTCCGAGACC 
AGCCTGGCCA CCTCCCTCAC CATGTCCCCT CCTCATCCAC ACCCCCACAG AGGGCCTGCC 
GGACACCTCC AGGAGGTCGA CCCACAGTCT GGATGGGCTA GCAGCCCAGA GCAGGAACAG 
GCCCGAGTGG AAGAGGAGGA AGATGAGGAG GAGGAGGTGG AAGAGGAAGA GGAGGAGGAG 
GAGGAAGTGG GGGACCGACA GAGGCTGAGG AAGAGAAATC GCAGGAGGGC CTACAGCCTG 
AGTTTGCTGG GGACGTCGGC AGACAGTGGG CTCTCTGTGA CGGCCGCTGA GCTGGAGGGT 
CGACTGTGGG ATGGAGCGGC CGGCAGCCCC CAGTACAGCA ACGCCCTTCA CCATTTATGG 
ATGATGACCT ACAGTCGCAG CACTCCGTAC CTGAGCAGCG ATGAATCCGA CAGCGATGGC 
GAGGAAATAC TGGAGGAGCT TCAGGAGCTC AGAGAGAAAC ATCTGACGGA GGTCCAGACC 
CTGCAGGCTG CCCAGAAGAG AGAGATCGAG GAGTTGTACG AGAAGATGGG GAAGGTCCCT 
CCGGCGGGTA TCGTCTCTCC CGCCGCCGTG CTGTCCAGAC GGCAGCGTCG CCTGTCAAAG 
GGTGGCGGCT TCCCCTCCTC TCGCAGGAAC AGCCTGCAGC GCTTGGACAT CCTTCCGCTC 
CAGGGTATCA TCAGGAGAAA CTCTTTAGGT GGCAGCAGCA GCGGCTCCCA GGATAAACCA 
AGCAAAGGCG TCACCTTTTC CAGCGATATC AGAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk4 ( XM_029850410.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029706270.1 Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo43123
Clone ID Related Accession (Same CDS sequence)	XM_029850413.1 , XM_029850410.1
Accession Version	XM_029850410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	serine/threonine-protein kinase WNK4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGA CTCGGAAGCT GACCAAGGCG GAGCGGCAGC GTTTCAGCGA GGAGGTGGAA 
ATGCTGAAGT GCCTGCAGCA CCCCAACATC GTCCGCTTCC ACGACTCCTG GAAGTCGACG 
GTGAAGGGCC TCAAATGCAT CATCCTGGTG ACGGAACTGA TGACGTCGGG CACGCTCAAA 
ACGTACTTGA AGAGATTCAA GGAGATGAAG CTAAAGCTGC TCCAGCGTTG GAGTCGACAA 
ATCCTCAAAG GGCTTCACTT CCTGCACACG CGAACGCCTC CCATCATCCA CAGAGACCTG 
AAGTGTGACA ACATCTTCAT CACGGGCCCC ACTGGCTCTG TCAAGATTGG AGATCTGGGA 
CTTGCCACTC TTAAAAGTGC CTCTTTTGCT AAAAGTGTCA TTGGAACGCC AGAGTTCATG 
GCCCCGGAGA TGTACGAGGA GAAGTACGAC GAAGCGGTGG ACGTCTACGC CTTCGGAATG 
TGCATCCTGG AGATGGCCAC CTCTGAGTAT CCGTACTCCG AGTGCCAAAA CGCCGCCCAG 
ATCTACCGCA AAGTCACCAG TGGAATTAAA CCAGACAGTT TCTACAAAGT CAAAGTCCCG 
GAGCTCAAAG AGATCATCGG GGGCTGCATT CGCATGAACA ACGATGAAAG GTACACCATT 
CAGGACCTCT TGGATCACCC CTTCTTCCAG GAGAACAACG GCGTGCACGT GGAGCTGGCG 
GAGGAGGACG ACATGGTGAA GCCAGGCCTG AAGCTGTGGC TGCGCATGGA CGACACAAAG 
AAGCTCCACG GGAAGTACAA GGACAACAAC GCCATCGAGT TCCTCTTTGA GCTCTACAAA 
GACGTGCCCG AGGAGGTGGC TCAGGAGATG GTGGTGCTCG GGTTCGTGTG CGAAGCCGAC 
TTCAAAATGG TGGCCAAGGC CATACGGGAC AGGGTGACAG CCATCAAGCG GCAAAGGGAG 
AAGATGAGGA AGCAGGCGGA GGAGCATAAG AAGAAGCAGC AGCAGGAAGC CATAGAAGAA 
GAGCCGGAAC CTCCGCCTGT TTTGCCCAAA GTTTTCGACG CCGTCCCGGT GGAGTCTCCA 
CCCCAAGCCC TAACCCCGCT GGCTCCCATC CTGTCCCCGG TCACCAGCTC CGTGGACTCT 
GGCGTCAGCT CCAGCTACCC CGCAGAGCCG GAGGAGCCTG AGGCCGACCA GCACTTCCAC 
ATGCGCCACA ACAGCCTGTC ATCTGCTAAC TCTGACTGCG AGACAGACGG CTATCTGAGC 
TCCTCTGGTC TTCAGGATCC ACTGGAGGCC GCCAGCTTTA ACTCGCAGGC CACACCTCCT 
GCCACATACA ACGAAGGCGC ACCAACTGGA AACGGTTTCC CCATCGCGGC TCTGCGCTTC 
CCCTCAAGCA TCGCTGTATC CAACAGCATA GAGAACGGAA ACTCGAGATC TCCGAGTGGC 
CTCAACTCAC CTGTGGACAG TTACGCCTCT GACGTGACCT CGGGTTTGAG TGATGGCTAC 
GAGGGCCTAT CGGAGAAGAG CGAGAAAACG GCCGCCAGGA AAACTGCTGG GAAGATGTTC 
AGAAGGAGGG CGCGATCAAG GCTGCGCATC ACAGGGCTCT CTGATAAAGT GGACCGGGTG 
GTGGAGTGCC AGCTGCAGAC GCACAACGAC AAGATGGTGA CGTTCAAGTT CGACCTGGAC 
GGAGACAATC CCGAAGACAT CGCCACTGTC ATGGTGCACA ATGAGTTCAT CCTGCCGTCG 
GAGAAAGAGG GCTTCATCCA CCGTATGGGG GACATCATCA AGCGTGCAGA GTCTCTGATG 
GCCAAAGGGC AGCCAGGCCA CGCCAGCGTG CACCGCCTCA CACAGCCCGC TTTCCACAGT 
GGGGCTAATT CACTGTCTTC GTCACAGCCT AATCTGCACA GCCACAACCG CCCTCGCACC 
AGCTCCTCCT CATCTCTACC TGACTTTAGC GTGGCTAACC CAAGAGCTGG GCTGGGTGGC 
CCCAGTGGCG ACATCTTCGG TGCAGACATT ACTTCACCTG AGCGACCTTT AATACGCTCA 
CAGTCTTTCC ACAACAGCCC AGGTTCTCCT CTCCATCATC ACCACCACTA CCATCCTGCA 
CCTGCCCTGC CGCACCACCA GCATTACCAC TTGCCTTACA GTAACTTCCC ATTCCCGTAC 
CCCGGCTCTC CAGGCTCCCC CAAGCCGGCC CACCACCCAC CGCTCACACG TGTAGCCAGT 
AACCCCACCT TCCCTTCCGT TCACTCCCCT GCCCCCGGTT CGCCCAATTC CCTCAATGAG 
GCCCCGCATC CAGGTAGCAA CGACAGTATC ACCCTGTCCC CACCTGTGAC TCAGCATCCC 
AACATGTGGC CCCCCCACAC GCAGCCCCTT TTTTCTTTAG CAAACGTGAT CTCCATGGCC 
ATGAGCATGG CCCAGTCTTT CATCCCTCCC ACTGGCCTGC CCACCCAGGG GATGGTCACC 
TATCCAGGCT TTCACCCACA GATGTCTGGT CCCATTCCCC CACAGACTGG TTACCCCTCC 
GTCTACCCAC CTCATTACCA GAGCCCACCA GGAGAGTTCT CTTACCCCTC AGTAGGCATC 
CCAGCACAGA ACAATGTCTA CCACAGGCCT GAGCCTCAGA TGGAAACTTT GCCCCAGTCC 
AGTAACCTCA ACTGGCAGCA TCCTGTCTCA CCCCCCTCAA TGGCTTCCAC CCCTCCCCTG 
GTTCCCCAGG AGCAGCAGGC GGGATTTTCC AATTCCCCCA GACTCACCCA GGAGGACCAT 
CTCCATGGAT ATGCGCCCCC ACATACAGCG CAGGTCCAAA CCCAGACTGA ACTCAGATCA 
GATGTTTCCA GCCCAAACTC TTTATCAGAT CTTTCTCCAG AGAGCACAAA AAGCACCTTG 
TCACCCTTTG CCAGTCAGCT CCTCCCCAGT GGAGCTGAGA TGACCACCAG TTCCCCCAAA 
CCAAAAACAC CTTGTAAGGA ATCTGGAGTC CAGCCTGCAC CAGTCACTGT TGGGAGATTC 
CAGGTGACGC CCAGCATGGA CATCCTGGCG CCTCCTGCCC CTGTCGTGTC CGCAGCGGCT 
GCAGAGAGCA GTGGCACCCC ATCAGAGAGC AGCACAGAGG AGCAGGTCGA GTCCGAGACC 
AGCCTGGCCA CCTCCCTCAC CATGTCCCCT CCTCATCCAC ACCCCCACAG AGGGCCTGCC 
GGACACCTCC AGGAGGTCGA CCCACAGTCT GGATGGGCTA GCAGCCCAGA GCAGGAACAG 
GCCCGAGTGG AAGAGGAGGA AGATGAGGAG GAGGAGGTGG AAGAGGAAGA GGAGGAGGAG 
GAGGAAGTGG GGGACCGACA GAGGCTGAGG AAGAGAAATC GCAGGAGGGC CTACAGCCTG 
AGTTTGCTGG GGACGTCGGC AGACAGTGGG CTCTCTGTGA CGGCCGCTGA GCTGGAGGGT 
CGACTGTGGG ATGGAGCGGC CGGCAGCCCC CAGTACAGCA ACGCCCTTCA CCATTTATGG 
ATGATGACCT ACAGTCGCAG CACTCCGTAC CTGAGCAGCG ATGAATCCGA CAGCGATGGC 
GAGGAAATAC TGGAGGAGCT TCAGGAGCTC AGAGAGAAAC ATCTGACGGA GGTCCAGACC 
CTGCAGGCTG CCCAGAAGAG AGAGATCGAG GAGTTGTACG AGAAGATGGG GAAGGTCCCT 
CCGGCGGGTA TCGTCTCTCC CGCCGCCGTG CTGTCCAGAC GGCAGCGTCG CCTGTCAAAG 
GGTGGCGGCT TCCCCTCCTC TCGCAGGAAC AGCCTGCAGC GCTTGGACAT CCTTCCGCTC 
CAGGGTATCA TCAGGAGAAA CTCTTTAGGT GGCAGCAGCA GCGGCTCCCA GGATAAACCA 
AGCAAAGGCG TCACCTTTTC CAGCGATATC AGAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029706270.1
CDS	276..4154
Translation	MIATRKLTKAERQRFSEEVEMLKCLQHPNIVRFHDSWKSTVKGLKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIGGCIRMNNDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKPGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKMVAKAIRDRVTAIKRQREKMRKQAEEHKKKQQQEAIEEEPEPPPVLPKVFDAVPVESPPQALTPLAPILSPVTSSVDSGVSSSYPAEPEEPEADQHFHMRHNSLSSANSDCETDGYLSSSGLQDPLEAASFNSQATPPATYNEGAPTGNGFPIAALRFPSSIAVSNSIENGNSRSPSGLNSPVDSYASDVTSGLSDGYEGLSEKSEKTAARKTAGKMFRRRARSRLRITGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIATVMVHNEFILPSEKEGFIHRMGDIIKRAESLMAKGQPGHASVHRLTQPAFHSGANSLSSSQPNLHSHNRPRTSSSSSLPDFSVANPRAGLGGPSGDIFGADITSPERPLIRSQSFHNSPGSPLHHHHHYHPAPALPHHQHYHLPYSNFPFPYPGSPGSPKPAHHPPLTRVASNPTFPSVHSPAPGSPNSLNEAPHPGSNDSITLSPPVTQHPNMWPPHTQPLFSLANVISMAMSMAQSFIPPTGLPTQGMVTYPGFHPQMSGPIPPQTGYPSVYPPHYQSPPGEFSYPSVGIPAQNNVYHRPEPQMETLPQSSNLNWQHPVSPPSMASTPPLVPQEQQAGFSNSPRLTQEDHLHGYAPPHTAQVQTQTELRSDVSSPNSLSDLSPESTKSTLSPFASQLLPSGAEMTTSSPKPKTPCKESGVQPAPVTVGRFQVTPSMDILAPPAPVVSAAAAESSGTPSESSTEEQVESETSLATSLTMSPPHPHPHRGPAGHLQEVDPQSGWASSPEQEQARVEEEEDEEEEVEEEEEEEEEVGDRQRLRKRNRRRAYSLSLLGTSADSGLSVTAAELEGRLWDGAAGSPQYSNALHHLWMMTYSRSTPYLSSDESDSDGEEILEELQELREKHLTEVQTLQAAQKREIEELYEKMGKVPPAGIVSPAAVLSRRQRRLSKGGGFPSSRRNSLQRLDILPLQGIIRRNSLGGSSSGSQDKPSKGVTFSSDIRM

Target ORF information:

RefSeq Version	XM_029850410.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes WNK lysine deficient protein kinase 4 (wnk4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029850410.1

ORF Insert Sequence:

ATGATTGCGA CTCGGAAGCT GACCAAGGCG GAGCGGCAGC GTTTCAGCGA GGAGGTGGAA 
ATGCTGAAGT GCCTGCAGCA CCCCAACATC GTCCGCTTCC ACGACTCCTG GAAGTCGACG 
GTGAAGGGCC TCAAATGCAT CATCCTGGTG ACGGAACTGA TGACGTCGGG CACGCTCAAA 
ACGTACTTGA AGAGATTCAA GGAGATGAAG CTAAAGCTGC TCCAGCGTTG GAGTCGACAA 
ATCCTCAAAG GGCTTCACTT CCTGCACACG CGAACGCCTC CCATCATCCA CAGAGACCTG 
AAGTGTGACA ACATCTTCAT CACGGGCCCC ACTGGCTCTG TCAAGATTGG AGATCTGGGA 
CTTGCCACTC TTAAAAGTGC CTCTTTTGCT AAAAGTGTCA TTGGAACGCC AGAGTTCATG 
GCCCCGGAGA TGTACGAGGA GAAGTACGAC GAAGCGGTGG ACGTCTACGC CTTCGGAATG 
TGCATCCTGG AGATGGCCAC CTCTGAGTAT CCGTACTCCG AGTGCCAAAA CGCCGCCCAG 
ATCTACCGCA AAGTCACCAG TGGAATTAAA CCAGACAGTT TCTACAAAGT CAAAGTCCCG 
GAGCTCAAAG AGATCATCGG GGGCTGCATT CGCATGAACA ACGATGAAAG GTACACCATT 
CAGGACCTCT TGGATCACCC CTTCTTCCAG GAGAACAACG GCGTGCACGT GGAGCTGGCG 
GAGGAGGACG ACATGGTGAA GCCAGGCCTG AAGCTGTGGC TGCGCATGGA CGACACAAAG 
AAGCTCCACG GGAAGTACAA GGACAACAAC GCCATCGAGT TCCTCTTTGA GCTCTACAAA 
GACGTGCCCG AGGAGGTGGC TCAGGAGATG GTGGTGCTCG GGTTCGTGTG CGAAGCCGAC 
TTCAAAATGG TGGCCAAGGC CATACGGGAC AGGGTGACAG CCATCAAGCG GCAAAGGGAG 
AAGATGAGGA AGCAGGCGGA GGAGCATAAG AAGAAGCAGC AGCAGGAAGC CATAGAAGAA 
GAGCCGGAAC CTCCGCCTGT TTTGCCCAAA GTTTTCGACG CCGTCCCGGT GGAGTCTCCA 
CCCCAAGCCC TAACCCCGCT GGCTCCCATC CTGTCCCCGG TCACCAGCTC CGTGGACTCT 
GGCGTCAGCT CCAGCTACCC CGCAGAGCCG GAGGAGCCTG AGGCCGACCA GCACTTCCAC 
ATGCGCCACA ACAGCCTGTC ATCTGCTAAC TCTGACTGCG AGACAGACGG CTATCTGAGC 
TCCTCTGGTC TTCAGGATCC ACTGGAGGCC GCCAGCTTTA ACTCGCAGGC CACACCTCCT 
GCCACATACA ACGAAGGCGC ACCAACTGGA AACGGTTTCC CCATCGCGGC TCTGCGCTTC 
CCCTCAAGCA TCGCTGTATC CAACAGCATA GAGAACGGAA ACTCGAGATC TCCGAGTGGC 
CTCAACTCAC CTGTGGACAG TTACGCCTCT GACGTGACCT CGGGTTTGAG TGATGGCTAC 
GAGGGCCTAT CGGAGAAGAG CGAGAAAACG GCCGCCAGGA AAACTGCTGG GAAGATGTTC 
AGAAGGAGGG CGCGATCAAG GCTGCGCATC ACAGGGCTCT CTGATAAAGT GGACCGGGTG 
GTGGAGTGCC AGCTGCAGAC GCACAACGAC AAGATGGTGA CGTTCAAGTT CGACCTGGAC 
GGAGACAATC CCGAAGACAT CGCCACTGTC ATGGTGCACA ATGAGTTCAT CCTGCCGTCG 
GAGAAAGAGG GCTTCATCCA CCGTATGGGG GACATCATCA AGCGTGCAGA GTCTCTGATG 
GCCAAAGGGC AGCCAGGCCA CGCCAGCGTG CACCGCCTCA CACAGCCCGC TTTCCACAGT 
GGGGCTAATT CACTGTCTTC GTCACAGCCT AATCTGCACA GCCACAACCG CCCTCGCACC 
AGCTCCTCCT CATCTCTACC TGACTTTAGC GTGGCTAACC CAAGAGCTGG GCTGGGTGGC 
CCCAGTGGCG ACATCTTCGG TGCAGACATT ACTTCACCTG AGCGACCTTT AATACGCTCA 
CAGTCTTTCC ACAACAGCCC AGGTTCTCCT CTCCATCATC ACCACCACTA CCATCCTGCA 
CCTGCCCTGC CGCACCACCA GCATTACCAC TTGCCTTACA GTAACTTCCC ATTCCCGTAC 
CCCGGCTCTC CAGGCTCCCC CAAGCCGGCC CACCACCCAC CGCTCACACG TGTAGCCAGT 
AACCCCACCT TCCCTTCCGT TCACTCCCCT GCCCCCGGTT CGCCCAATTC CCTCAATGAG 
GCCCCGCATC CAGGTAGCAA CGACAGTATC ACCCTGTCCC CACCTGTGAC TCAGCATCCC 
AACATGTGGC CCCCCCACAC GCAGCCCCTT TTTTCTTTAG CAAACGTGAT CTCCATGGCC 
ATGAGCATGG CCCAGTCTTT CATCCCTCCC ACTGGCCTGC CCACCCAGGG GATGGTCACC 
TATCCAGGCT TTCACCCACA GATGTCTGGT CCCATTCCCC CACAGACTGG TTACCCCTCC 
GTCTACCCAC CTCATTACCA GAGCCCACCA GGAGAGTTCT CTTACCCCTC AGTAGGCATC 
CCAGCACAGA ACAATGTCTA CCACAGGCCT GAGCCTCAGA TGGAAACTTT GCCCCAGTCC 
AGTAACCTCA ACTGGCAGCA TCCTGTCTCA CCCCCCTCAA TGGCTTCCAC CCCTCCCCTG 
GTTCCCCAGG AGCAGCAGGC GGGATTTTCC AATTCCCCCA GACTCACCCA GGAGGACCAT 
CTCCATGGAT ATGCGCCCCC ACATACAGCG CAGGTCCAAA CCCAGACTGA ACTCAGATCA 
GATGTTTCCA GCCCAAACTC TTTATCAGAT CTTTCTCCAG AGAGCACAAA AAGCACCTTG 
TCACCCTTTG CCAGTCAGCT CCTCCCCAGT GGAGCTGAGA TGACCACCAG TTCCCCCAAA 
CCAAAAACAC CTTGTAAGGA ATCTGGAGTC CAGCCTGCAC CAGTCACTGT TGGGAGATTC 
CAGGTGACGC CCAGCATGGA CATCCTGGCG CCTCCTGCCC CTGTCGTGTC CGCAGCGGCT 
GCAGAGAGCA GTGGCACCCC ATCAGAGAGC AGCACAGAGG AGCAGGTCGA GTCCGAGACC 
AGCCTGGCCA CCTCCCTCAC CATGTCCCCT CCTCATCCAC ACCCCCACAG AGGGCCTGCC 
GGACACCTCC AGGAGGTCGA CCCACAGTCT GGATGGGCTA GCAGCCCAGA GCAGGAACAG 
GCCCGAGTGG AAGAGGAGGA AGATGAGGAG GAGGAGGTGG AAGAGGAAGA GGAGGAGGAG 
GAGGAAGTGG GGGACCGACA GAGGCTGAGG AAGAGAAATC GCAGGAGGGC CTACAGCCTG 
AGTTTGCTGG GGACGTCGGC AGACAGTGGG CTCTCTGTGA CGGCCGCTGA GCTGGAGGGT 
CGACTGTGGG ATGGAGCGGC CGGCAGCCCC CAGTACAGCA ACGCCCTTCA CCATTTATGG 
ATGATGACCT ACAGTCGCAG CACTCCGTAC CTGAGCAGCG ATGAATCCGA CAGCGATGGC 
GAGGAAATAC TGGAGGAGCT TCAGGAGCTC AGAGAGAAAC ATCTGACGGA GGTCCAGACC 
CTGCAGGCTG CCCAGAAGAG AGAGATCGAG GAGTTGTACG AGAAGATGGG GAAGGTCCCT 
CCGGCGGGTA TCGTCTCTCC CGCCGCCGTG CTGTCCAGAC GGCAGCGTCG CCTGTCAAAG 
GGTGGCGGCT TCCCCTCCTC TCGCAGGAAC AGCCTGCAGC GCTTGGACAT CCTTCCGCTC 
CAGGGTATCA TCAGGAGAAA CTCTTTAGGT GGCAGCAGCA GCGGCTCCCA GGATAAACCA 
AGCAAAGGCG TCACCTTTTC CAGCGATATC AGAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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wnk4 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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